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<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETK8</accession>
    <name>A0A1U7ETK8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Archaeal histone</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">hstA</name>
        <name evidence="8" type="ordered locus">NP_0316A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the archaeal histone HMF family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321887.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETK8"/>
    <dbReference type="STRING" id="348780.NP_0316A"/>
    <dbReference type="EnsemblBacteria" id="CAI48249">
        <property type="protein sequence ID" value="CAI48249"/>
        <property type="gene ID" value="NP_0316A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702833"/>
    <dbReference type="KEGG" id="nph:NP_0316A"/>
    <dbReference type="eggNOG" id="arCOG02144">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1801751_0_0_2"/>
    <dbReference type="OrthoDB" id="7514at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22909">
        <property type="entry name" value="HFD_archaea_histone-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050947">
        <property type="entry name" value="Archaeal_histone_HMF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050004">
        <property type="entry name" value="HmfB-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF043032">
        <property type="entry name" value="archaea_histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47828">
        <property type="entry name" value="ARCHAEAL HISTONE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47828:SF1">
        <property type="entry name" value="ARCHAEAL HISTONE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="7">
        <location>
            <begin position="4"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="7">
        <location>
            <begin position="74"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI48249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="145" mass="15907" checksum="14EA23EC27F960F7" modified="2017-05-10" version="1">MSVELPFAPVDTIIRRNAGQLRVSAEAAEELARRIQDHGAALAETAAERATADGRKTLMAEDFGSTPGDKDELELPVAPVDRIARLDIDDRYRVSMDARIALAGELEAYADTVATAAAILARHADRRTVKADDIETYYELKPHFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETT0</accession>
    <name>A0A1U7ETT0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ferredoxin (4Fe-4S)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ferC</name>
        <name evidence="6" type="ordered locus">NP_0494A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321974.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETT0"/>
    <dbReference type="STRING" id="348780.NP_0494A"/>
    <dbReference type="EnsemblBacteria" id="CAI48338">
        <property type="protein sequence ID" value="CAI48338"/>
        <property type="gene ID" value="NP_0494A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702707"/>
    <dbReference type="KEGG" id="nph:NP_0494A"/>
    <dbReference type="eggNOG" id="arCOG00349">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2565678_0_0_2"/>
    <dbReference type="OrthoDB" id="5583at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051269">
        <property type="entry name" value="Fe-S_cluster_ET"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36923">
        <property type="entry name" value="FERREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36923:SF3">
        <property type="entry name" value="FERREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13459">
        <property type="entry name" value="Fer4_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI48338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="80" mass="9040" checksum="774E998643C0DD65" modified="2017-05-10" version="1">MKVRFDRETCIGMFQCVAEWDAFEEDEDEGKADLDGAEEVAPGIFEREVPEDAEFDAKFAARSCPVDAIEIYDEDGEQLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETT7</accession>
    <name>A0A1U7ETT7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphate ABC transporter permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0510A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321982.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETT7"/>
    <dbReference type="STRING" id="348780.NP_0510A"/>
    <dbReference type="EnsemblBacteria" id="CAI48346">
        <property type="protein sequence ID" value="CAI48346"/>
        <property type="gene ID" value="NP_0510A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700878"/>
    <dbReference type="KEGG" id="nph:NP_0510A"/>
    <dbReference type="eggNOG" id="arCOG08970">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110113_0_0_2"/>
    <dbReference type="OrthoDB" id="331535at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="219" mass="21644" checksum="4B6AB7D3E51D5376" modified="2017-05-10" version="1">MDLIAAGVVVAGVVLLFAGAALSVYATALLGVLIGGGIGYVAAPQVLGAVGAEGIVGLVAVIAVGGAFGALSAYLALSFATAIPGFVVGAYIGLYVITPLFTEGGLVRYLVLLLGGVGGALVAFTATKIALVFITAFIGATLASRAVTVEDVTAAREAFSLDPILFDPLGTTALVGIQVPLFGVLFVLGVLSQVGLFKLGWVGRLAGVLPGVGRVVGDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETU9</accession>
    <name>A0A1U7ETU9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Probable phosphoribosyltransferase (Homolog to anthranilate phosphoribosyltransferase)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">trpD2</name>
        <name evidence="8" type="ordered locus">NP_0532A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321993.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETU9"/>
    <dbReference type="STRING" id="348780.NP_0532A"/>
    <dbReference type="EnsemblBacteria" id="CAI48357">
        <property type="protein sequence ID" value="CAI48357"/>
        <property type="gene ID" value="NP_0532A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703412"/>
    <dbReference type="KEGG" id="nph:NP_0532A"/>
    <dbReference type="eggNOG" id="arCOG02012">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034315_0_0_2"/>
    <dbReference type="OrthoDB" id="233227at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004048">
        <property type="term" value="F:anthranilate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1030.10:FF:000010">
        <property type="entry name" value="Anthranilate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1030.10">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.970.10">
        <property type="entry name" value="Transferase, Pyrimidine Nucleoside Phosphorylase, Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005940">
        <property type="entry name" value="Anthranilate_Pribosyl_Tfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000312">
        <property type="entry name" value="Glycosyl_Trfase_fam3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017459">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036320">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035902">
        <property type="entry name" value="Nuc_phospho_transferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43285">
        <property type="entry name" value="ANTHRANILATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43285:SF2">
        <property type="entry name" value="ANTHRANILATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02885">
        <property type="entry name" value="Glycos_trans_3N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00591">
        <property type="entry name" value="Glycos_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52418">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47648">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="2 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Glycosyl transferase family 3 N-terminal" evidence="7">
        <location>
            <begin position="28"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 3" evidence="6">
        <location>
            <begin position="107"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI48357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="358" mass="38836" checksum="443F68E8CA28EFF6" modified="2017-05-10" version="1">MLTEKQEFGDWPLKRLMTEVVGTGIKSAEDMSREQATDAFRRIVAGEPDETTLGAFWLANRWKRNTPEELAAFLDVMLESVERAEPKADPVDCGANYDGKAETAILGVAAGAVAAGAGTPVVVHGGDRIPSQKSPAYKDVLDELGVTTELDPEESAAMVDETGFGFYFQPAFNPAIDDLYDRRDEMGVRTFVNTIETLANPAGASVHLGSFYHLTFAEKVIDTIRAAERSRFDRAILFQGMEGYDDIRPGYTKVAEWADDGALDDVMNDYEIETAELGMDFEYDDLAVDDIAADSAAITEAVLTGERTDQFRDAVLLNGAFRIYAGGDADSFEAGLDAAERALDDGSAAAVLTDLQSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETY5</accession>
    <name>A0A1U7ETY5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DUF151 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0634A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322044.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETY5"/>
    <dbReference type="STRING" id="348780.NP_0634A"/>
    <dbReference type="EnsemblBacteria" id="CAI48408">
        <property type="protein sequence ID" value="CAI48408"/>
        <property type="gene ID" value="NP_0634A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700913"/>
    <dbReference type="KEGG" id="nph:NP_0634A"/>
    <dbReference type="eggNOG" id="arCOG01759">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096111_3_0_2"/>
    <dbReference type="OrthoDB" id="30741at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.690.10">
        <property type="entry name" value="Bifunctional nuclease domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036104">
        <property type="entry name" value="BFN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003729">
        <property type="entry name" value="Bi_nuclease_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15160:SF1">
        <property type="entry name" value="VON HIPPEL-LINDAU DISEASE TUMOR SUPPRESSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15160">
        <property type="entry name" value="VON HIPPEL-LINDAU PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02577">
        <property type="entry name" value="BFN_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103256">
        <property type="entry name" value="Hypothetical protein TM0160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51658">
        <property type="entry name" value="BFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BFN" evidence="2">
        <location>
            <begin position="2"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="147" mass="16131" checksum="B83330007C7D2012" modified="2017-05-10" version="1">MAHKATVHGLGVSVDEDDDSEEGPPVVVLHARDEALPIFISEDQAKSISHALDDEPFQRPLTHDLFIEMLTEFGGAIDRVRIDDLADSTFLAKIDGERYSDGERTTVTFDARPSDAIAVALRVDCPILIGDEVLDEAGQPQDTFEVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU06</accession>
    <name>A0A1U7EU06_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable beta-carotene 15,15'-dioxygenase</fullName>
            <ecNumber evidence="1">1.13.11.63</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">blh</name>
        <name evidence="2" type="ordered locus">NP_0650A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the cleavage of beta-carotene at its central double bond (15,15') to yield two molecules of all-trans-retinal.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>all-trans-beta-carotene + O2 = 2 all-trans-retinal</text>
            <dbReference type="Rhea" id="RHEA:32887"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17579"/>
            <dbReference type="ChEBI" id="CHEBI:17898"/>
            <dbReference type="EC" id="1.13.11.63"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Brp/Blh beta-carotene diooxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.63" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322052.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU06"/>
    <dbReference type="STRING" id="348780.NP_0650A"/>
    <dbReference type="EnsemblBacteria" id="CAI48416">
        <property type="protein sequence ID" value="CAI48416"/>
        <property type="gene ID" value="NP_0650A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702558"/>
    <dbReference type="KEGG" id="nph:NP_0650A"/>
    <dbReference type="eggNOG" id="arCOG02947">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068196_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003834">
        <property type="term" value="F:beta-carotene 15,15'-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010436">
        <property type="term" value="F:carotenoid dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016121">
        <property type="term" value="P:carotene catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02093">
        <property type="entry name" value="Beta_carotene_diox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022270">
        <property type="entry name" value="Blh_diox"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03753">
        <property type="entry name" value="blh_monoox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15461">
        <property type="entry name" value="BCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="1" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="233"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="285"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="318"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="350" mass="36723" checksum="367F2E22E44098B7" modified="2017-05-10" version="1">MSNASLRPSGTASATLFRLAFLPGWAVIAATTGAFLVGASLPLTYQLIPLAASVVLLGLPHGAVDHLALPRTRNERVTVRWLAAIGVLYAVVGGLYAAVWFLAPVGAVAAFIFMTWVHWGQGEIYPLVALADADHLDGRLERGLTAAIRGALPMLVPFVAFPDQYELVVTTLVGLFDADAAATAAAAFTPTARLAVAVTVGGLVAVTLGIGAVAASETGWGPWLLDAGETGLLILFFAAVPPIFAIGLYFCFWHSLRHIVRLLAVDNRAAPALDGRRYGAALARFARDAAPLSAASLVLLGLLYLAVPGSVDSPLSLVGTYLVLIAVLTLPHVVVVAWMDHEQRLWRPGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU34</accession>
    <name>A0A1U7EU34_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">IS1341-type transposase ISNph21</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0706A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322080.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU34"/>
    <dbReference type="STRING" id="348780.NP_0706A"/>
    <dbReference type="EnsemblBacteria" id="CAI48444">
        <property type="protein sequence ID" value="CAI48444"/>
        <property type="gene ID" value="NP_0706A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700936"/>
    <dbReference type="KEGG" id="nph:NP_0706A"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_3_3_2"/>
    <dbReference type="OrthoDB" id="210698at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF26">
        <property type="entry name" value="TRANSPOSASE, PROBABLY IS605-TNPB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas12f1-like TNB" evidence="2">
        <location>
            <begin position="296"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="402" mass="46015" checksum="F9729EAC877675B0" modified="2017-05-10" version="1">MEYRRTAVIKLDTPEGADTHLRETVEQFKYCANTASEWCWHGDDGYHVTSKAKAERALYDQLREDTELTANLVQKGIRQAVESVKSGVERLKNDPDTSRPTFTADTAVYDKRSATFHRDHASLSTPDGRIECDYILPDDADTPPTKYVTDEDYEFRRATLHRRDSDWYLHASMLKEDDDTEPTTGHSTVLGVDLGVNQLAVASTGRFWSADEFNHWKREYEKRRGDLQQCGTRAAHDAIAGVERKEDGRFEIFLHRVANEIVAEAVEHDCSHIVFEDLTDIRENVPEASWHHLWAFRRLYEYVEYKAREQGVKVVQVDPRNTSKRCSTCGFTHDDNRHGEDFECQDCGYQNHADYNASKNIGLQYLRRRQNADDGGAPVDVRLNRGTLNVSGEYDLPASSEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU39</accession>
    <name>A0A1U7EU39_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC-type transport system periplasmic substrate-binding protein (Probable substrate dipeptide/oligopeptide)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">dppA1</name>
        <name evidence="6" type="synonym">abc01s</name>
        <name evidence="6" type="ordered locus">NP_0758A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial solute-binding protein 5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322106.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU39"/>
    <dbReference type="STRING" id="348780.NP_0758A"/>
    <dbReference type="EnsemblBacteria" id="CAI48470">
        <property type="protein sequence ID" value="CAI48470"/>
        <property type="gene ID" value="NP_0758A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702366"/>
    <dbReference type="KEGG" id="nph:NP_0758A"/>
    <dbReference type="eggNOG" id="arCOG01534">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017028_7_4_2"/>
    <dbReference type="OrthoDB" id="194307at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904680">
        <property type="term" value="F:peptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.76.10">
        <property type="entry name" value="Dipeptide-binding Protein, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.105.10">
        <property type="entry name" value="Dipeptide-binding Protein, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290:SF9">
        <property type="entry name" value="OLIGOPEPTIDE-BINDING PROTEIN APPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PERIPLASMIC BINDING COMPONENT OF ABC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Solute-binding protein family 5" evidence="5">
        <location>
            <begin position="165"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="27"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="31"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI48470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="546" mass="60134" checksum="A66BBA158B589F7D" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU51</accession>
    <name>A0A1U7EU51_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HAD superfamily hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0770A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HAD-like hydrolase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322112.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU51"/>
    <dbReference type="STRING" id="348780.NP_0770A"/>
    <dbReference type="EnsemblBacteria" id="CAI48476">
        <property type="protein sequence ID" value="CAI48476"/>
        <property type="gene ID" value="NP_0770A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700956"/>
    <dbReference type="KEGG" id="nph:NP_0770A"/>
    <dbReference type="eggNOG" id="arCOG04692">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082635_0_0_2"/>
    <dbReference type="OrthoDB" id="8384at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008967">
        <property type="term" value="F:phosphoglycolate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01427">
        <property type="entry name" value="HAD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050155">
        <property type="entry name" value="HAD-like_hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006438">
        <property type="entry name" value="HAD-SF_TIGR01548"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01548">
        <property type="entry name" value="HAD-SF-IA-hyp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43434">
        <property type="entry name" value="PHOSPHOGLYCOLATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43434:SF1">
        <property type="entry name" value="PHOSPHOGLYCOLATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="287" mass="31540" checksum="37F43C87312FB0DA" modified="2017-05-10" version="1">MNVDSVILDIDGVLVDVEDSYRRAIVESIAHVYGDTIDKADIQLFKDAGGFNNDWELTYAAALYVLARREKPALSIETYTGLIAASGGGLQAAETAIADELPPAAREKVLAEWDRKRLRDVFQQLYLGSELYRELESGEPDLDTEGFIHDEPVLLAPDTREALEEWSLGVLTGRPAAEAEIALDRVGLDIPETHRFTMDDWEEGKPHPHGLVELTKRFDAESAVFAGDTLDDIRTARNAAEDDPDREFYGVGVLTGGLSGDEGRRKFRNAGADIIIETINDLPGLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU68</accession>
    <name>A0A1U7EU68_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0146 protein NP_0772A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0772A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the UPF0146 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322113.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU68"/>
    <dbReference type="STRING" id="348780.NP_0772A"/>
    <dbReference type="EnsemblBacteria" id="CAI48477">
        <property type="protein sequence ID" value="CAI48477"/>
        <property type="gene ID" value="NP_0772A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700957"/>
    <dbReference type="KEGG" id="nph:NP_0772A"/>
    <dbReference type="eggNOG" id="arCOG04385">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148458_1_0_2"/>
    <dbReference type="OrthoDB" id="59816at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00341">
        <property type="entry name" value="UPF0146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005353">
        <property type="entry name" value="UPF0146"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03686">
        <property type="entry name" value="UPF0146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="128" mass="13527" checksum="792C6651D08C815C" modified="2017-05-10" version="1">MHTDSEGALVDRLSSYDALVEVGVGNRPTVAAALDERGCRVTATDVDTCAVPGTVRFVRDDVTDPDVSVYSGAGAVYALNCPPELQRPLVSLASRVDADCLFTTLGGDPTLVDATPETLPTQTLYHYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU88</accession>
    <name>A0A1U7EU88_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Pyridoxal phosphate-dependent aminotransferase</fullName>
            <ecNumber evidence="8">2.6.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">aspC4</name>
        <name evidence="8" type="ordered locus">NP_0824A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="8"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322139.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU88"/>
    <dbReference type="STRING" id="348780.NP_0824A"/>
    <dbReference type="EnsemblBacteria" id="CAI48503">
        <property type="protein sequence ID" value="CAI48503"/>
        <property type="gene ID" value="NP_0824A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702536"/>
    <dbReference type="KEGG" id="nph:NP_0824A"/>
    <dbReference type="eggNOG" id="arCOG01134">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_4_3_2"/>
    <dbReference type="OrthoDB" id="372018at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050596">
        <property type="entry name" value="AspAT/PAT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383:SF3">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="7">
        <location>
            <begin position="22"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI48503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="368" mass="40274" checksum="21885BE10F4F1990" modified="2017-05-10" version="1">MGDYDRPVFFHLMEYAANADRDVVDMVSGNPDWEPPEALREGLAAYVSADVDTFQYPPSEGLAELRAEIAARRGVDRERVVVTNGAGEANYLAMAEALESQPGDEILLVDPVYPYYHKRTELLGGTPTFVAADADGRVDPAAVRAAASDETAAIVVNSPNNPTGAVYGRETKQELVDIAERHDALLVSDEVYDHFVYEPDAFESALAFDSANVAVTNAFSKSMAITGFRVGYGVYPERLVDDIRTRHMLVNVAGSRPAQHAVLHALRETPAEYYERNRSMLEERIDAFCAALDAAGAEYTRPQGAFYVMARFDGYPGTLAHTERLIDEAGVAGMPGDAFGESRAEWLRFAVVTPRAEEAAERLANYFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUC9</accession>
    <name>A0A1U7EUC9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Probable S-adenosylmethionine-dependent methyltransferase (Homolog to 24-sterol C-methyltransferase)</fullName>
            <ecNumber evidence="3">2.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0956A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322205.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUC9"/>
    <dbReference type="STRING" id="348780.NP_0956A"/>
    <dbReference type="EnsemblBacteria" id="CAI48569">
        <property type="protein sequence ID" value="CAI48569"/>
        <property type="gene ID" value="NP_0956A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701010"/>
    <dbReference type="KEGG" id="nph:NP_0956A"/>
    <dbReference type="eggNOG" id="arCOG01781">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085884_0_0_2"/>
    <dbReference type="OrthoDB" id="4832at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003838">
        <property type="term" value="F:sterol 24-C-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050447">
        <property type="entry name" value="Erg6_SMT_methyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44068:SF1">
        <property type="entry name" value="HYPOTHETICAL LOC100005854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44068">
        <property type="entry name" value="ZGC:194242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="2">
        <location>
            <begin position="43"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="227" mass="25815" checksum="46F4853AEFFA501D" modified="2017-05-10" version="1">MSVRAEFDAWAADGRDRGMEQRHWHTAKHALARMPVEADETVLDLGCGSGYAARALRDAKDAGRAYGLDGSPEMVRNAREYTDDPQVSYVVGDFGSLPFAADSIDHAFSMEAFYYAADPVGALNELRRVLRPGGTFYCAVDYFEESVHTHEWQEGIDIDMTLWDYEQYREAFREAGFYVAEQDTIPDRETEIPPEDEFPTDDWERREDMVERFREFGTLLTVGVAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUK5</accession>
    <name>A0A1U7EUK5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Probable sulfite/nitrite reductase (Ferredoxin)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">nirA2</name>
        <name evidence="10" type="ordered locus">NP_1146A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322300.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUK5"/>
    <dbReference type="STRING" id="348780.NP_1146A"/>
    <dbReference type="EnsemblBacteria" id="CAI48664">
        <property type="protein sequence ID" value="CAI48664"/>
        <property type="gene ID" value="NP_1146A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702976"/>
    <dbReference type="KEGG" id="nph:NP_1146A"/>
    <dbReference type="eggNOG" id="arCOG02055">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015667_2_3_2"/>
    <dbReference type="OrthoDB" id="15347at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.480.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.413.10">
        <property type="entry name" value="Sulfite Reductase Hemoprotein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051329">
        <property type="entry name" value="NIR_SIR_4Fe-4S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005117">
        <property type="entry name" value="NiRdtase/SiRdtase_haem-b_fer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036136">
        <property type="entry name" value="Nit/Sulf_reduc_fer-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006067">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045854">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006066">
        <property type="entry name" value="NO2/SO3_Rdtase_FeS/sirohaem_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32439">
        <property type="entry name" value="FERREDOXIN--NITRITE REDUCTASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32439:SF0">
        <property type="entry name" value="FERREDOXIN--NITRITE REDUCTASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01077">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03460">
        <property type="entry name" value="NIR_SIR_ferr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00397">
        <property type="entry name" value="SIROHAEM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56014">
        <property type="entry name" value="Nitrite and sulphite reductase 4Fe-4S domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55124">
        <property type="entry name" value="Nitrite/Sulfite reductase N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitrite/Sulfite reductase ferredoxin-like" evidence="9">
        <location>
            <begin position="52"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Nitrite/Sulfite reductase ferredoxin-like" evidence="9">
        <location>
            <begin position="95"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Nitrite/sulphite reductase 4Fe-4S" evidence="8">
        <location>
            <begin position="137"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Nitrite/Sulfite reductase ferredoxin-like" evidence="9">
        <location>
            <begin position="326"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Nitrite/sulphite reductase 4Fe-4S" evidence="8">
        <location>
            <begin position="402"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI48664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="584" mass="65802" checksum="51070F260100501D" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUL5</accession>
    <name>A0A1U7EUL5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Probable ferredoxin (4Fe-4S)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1112A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322283.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUL5"/>
    <dbReference type="STRING" id="348780.NP_1112A"/>
    <dbReference type="EnsemblBacteria" id="CAI48647">
        <property type="protein sequence ID" value="CAI48647"/>
        <property type="gene ID" value="NP_1112A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701056"/>
    <dbReference type="KEGG" id="nph:NP_1112A"/>
    <dbReference type="eggNOG" id="arCOG00349">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2419925_0_0_2"/>
    <dbReference type="OrthoDB" id="241187at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13459">
        <property type="entry name" value="Fer4_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="74" mass="7845" checksum="80531EEB2730F073" modified="2017-05-10" version="1">MAYEITVDMDACEGIFACLVRDDRFAEAEDGLVTLPGGERTEGILSATFDDDQREAAEQAAAACPTDAIEVSDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUS2</accession>
    <name>A0A1U7EUS2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Tfx-type DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">tfx</name>
        <name evidence="2" type="ordered locus">NP_1296A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322374.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUS2"/>
    <dbReference type="STRING" id="348780.NP_1296A"/>
    <dbReference type="EnsemblBacteria" id="CAI48739">
        <property type="protein sequence ID" value="CAI48739"/>
        <property type="gene ID" value="NP_1296A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701106"/>
    <dbReference type="KEGG" id="nph:NP_1296A"/>
    <dbReference type="eggNOG" id="arCOG04554">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125807_0_0_2"/>
    <dbReference type="OrthoDB" id="17771at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1190.10">
        <property type="entry name" value="DNA-binding protein Tfx superfamily, archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029291">
        <property type="entry name" value="Tfx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004645">
        <property type="entry name" value="Tfx_DNA-bd_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036657">
        <property type="entry name" value="Tfx_DNA-bd_sf_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003054">
        <property type="entry name" value="PRK03975.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00721">
        <property type="entry name" value="tfx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14601">
        <property type="entry name" value="TFX_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89915">
        <property type="entry name" value="DNA-binding protein Tfx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA binding protein Tfx C-terminal" evidence="1">
        <location>
            <begin position="67"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="151" mass="16194" checksum="01AFAF5C932B81C2" modified="2017-05-10" version="1">MTLPDPDELLDRVGFEEATNVLTRRQAEVLALRERDIPQADIAGLLGTSRANVSSVEASARENIAKAEETVAFVEALSAPVQLTIESGTDLYDVPNRVYSACDEADVKVSRTAPELMKLISDAAGDAIRGREVRHELTVTVAGDGSVSVRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUS4</accession>
    <name>A0A1U7EUS4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glutaredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">grx1</name>
        <name evidence="2" type="ordered locus">NP_1250A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322352.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUS4"/>
    <dbReference type="STRING" id="348780.NP_1250A"/>
    <dbReference type="EnsemblBacteria" id="CAI48716">
        <property type="protein sequence ID" value="CAI48716"/>
        <property type="gene ID" value="NP_1250A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702762"/>
    <dbReference type="KEGG" id="nph:NP_1250A"/>
    <dbReference type="eggNOG" id="arCOG02608">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026126_8_2_2"/>
    <dbReference type="OrthoDB" id="197796at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011767">
        <property type="entry name" value="GLR_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45288">
        <property type="entry name" value="THIOREDOXIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45288:SF1">
        <property type="entry name" value="THIOREDOXIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00195">
        <property type="entry name" value="GLUTAREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="1">
        <location>
            <begin position="2"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="82" mass="9123" checksum="75282DF392EF122F" modified="2017-05-10" version="1">MANITLYELAGCPYCKKVIDKLDELGLDYDSIEVPRSHSDRTEVEDVSGQTGVPVIIDEEHGIEGMPESDDIVEYLEETYGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUV7</accession>
    <name>A0A1U7EUV7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">START domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1380A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322416.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUV7"/>
    <dbReference type="SMR" id="A0A1U7EUV7"/>
    <dbReference type="STRING" id="348780.NP_1380A"/>
    <dbReference type="EnsemblBacteria" id="CAI48781">
        <property type="protein sequence ID" value="CAI48781"/>
        <property type="gene ID" value="NP_1380A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701133"/>
    <dbReference type="KEGG" id="nph:NP_1380A"/>
    <dbReference type="eggNOG" id="arCOG02899">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1507348_0_0_2"/>
    <dbReference type="OrthoDB" id="167073at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07812">
        <property type="entry name" value="SRPBCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019587">
        <property type="entry name" value="Polyketide_cyclase/dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10604">
        <property type="entry name" value="Polyketide_cyc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="173" mass="19782" checksum="AA95748EE5AAB9A8" modified="2017-05-10" version="1">MDRLEVSTNVYLPPQEVYEFLLCFREYAGYTEHLREVRQHGDGSPGTQYELDFSWWRLDYTARSEVTAVEPPGDADPPYRIDWRAVEDIAAAGSWLVEPVDPSGPETDVTFAVEYAPQSADTSRLELPRFVPFDAVVERAKPYIENEAERLVKRVVADLEGQKRDVALEIETQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUY1</accession>
    <name>A0A1U7EUY1_NATPD</name>
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        <submittedName>
            <fullName evidence="8">GtrA family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">NP_1434A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GtrA family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUY1"/>
    <dbReference type="STRING" id="348780.NP_1434A"/>
    <dbReference type="EnsemblBacteria" id="CAI48808">
        <property type="protein sequence ID" value="CAI48808"/>
        <property type="gene ID" value="NP_1434A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701149"/>
    <dbReference type="KEGG" id="nph:NP_1434A"/>
    <dbReference type="eggNOG" id="arCOG02228">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083873_6_1_2"/>
    <dbReference type="OrthoDB" id="44002at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051401">
        <property type="entry name" value="GtrA_CellWall_Glycosyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007267">
        <property type="entry name" value="GtrA_DPMS_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38459">
        <property type="entry name" value="PROPHAGE BACTOPRENOL-LINKED GLUCOSE TRANSLOCASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38459:SF1">
        <property type="entry name" value="PROPHAGE BACTOPRENOL-LINKED GLUCOSE TRANSLOCASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04138">
        <property type="entry name" value="GtrA_DPMS_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="GtrA/DPMS transmembrane" evidence="7">
        <location>
            <begin position="18"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI48808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="151" mass="16284" checksum="62F48B5D7FE9F6A5" modified="2017-05-10" version="1">MTRRQIRETLLSPVRFGQFAGVGAVGAVCDNIVLLTLATAGVTPELAKFVGIEVAIVVMFLLNERWTFSEAGAPGPVAFLRRLGTSNVVRVGGIAVQLVVFSVVYRWLHVEVGLFGVDLWLLVASGAGIACGMVVNYVTESLLTWRVHNSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV17</accession>
    <name>A0A1U7EV17_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Abi/CAAX domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_1458A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322455.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV17"/>
    <dbReference type="STRING" id="348780.NP_1458A"/>
    <dbReference type="EnsemblBacteria" id="CAI48820">
        <property type="protein sequence ID" value="CAI48820"/>
        <property type="gene ID" value="NP_1458A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701155"/>
    <dbReference type="KEGG" id="nph:NP_1458A"/>
    <dbReference type="eggNOG" id="arCOG02766">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073769_0_0_2"/>
    <dbReference type="OrthoDB" id="275779at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004637">
        <property type="term" value="F:phosphoribosylamine-glycine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080120">
        <property type="term" value="P:CAAX-box protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052710">
        <property type="entry name" value="CAAX_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020559">
        <property type="entry name" value="PRibGlycinamide_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1/LyrA-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36435:SF1">
        <property type="entry name" value="CAAX AMINO TERMINAL PROTEASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36435">
        <property type="entry name" value="SLR1288 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00184">
        <property type="entry name" value="GARS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="CAAX prenyl protease 2/Lysostaphin resistance protein A-like" evidence="2">
        <location>
            <begin position="140"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="263" mass="27269" checksum="CD81A3BC2FCA9256" modified="2017-05-10" version="1">MSLHSRVPGPIVAVGVAIGLAAVAYGGGNIVAAGVAFGLASAGIELTLRQSFILSVVVLQLVFFTGISLVYLNYRSLSLSDIGVRVPGLEGWIVLGAGFIGMILLWFVASVASVIISSRFGIEQEQQAIFEMAQQDPFIFLLLGTLSLLIVGPTEELLFRGVIQTRLRETFGVASGLTLATALFALIHIGGFGISASGLLGVSVLFVVGLVLAVAYEYTGNLVVVAVMHGLFNATQATLGYIGVRFGDPEAMVLLAGAVVGAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV32</accession>
    <name>A0A1U7EV32_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HTH domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1562A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322506.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV32"/>
    <dbReference type="STRING" id="348780.NP_1562A"/>
    <dbReference type="EnsemblBacteria" id="CAI48872">
        <property type="protein sequence ID" value="CAI48872"/>
        <property type="gene ID" value="NP_1562A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701184"/>
    <dbReference type="KEGG" id="nph:NP_1562A"/>
    <dbReference type="eggNOG" id="arCOG03067">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124803_2_1_2"/>
    <dbReference type="OrthoDB" id="10985at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12840">
        <property type="entry name" value="HTH_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="125" mass="13629" checksum="B5376D189D972220" modified="2017-05-10" version="1">MSAELHRNDPTRTTQRDTATPGDCEAVLAALEDPDCRALLEATTDEALTAQELTERCDIPRSTTYRKVEQLTDAGLLEERVRISTNGKHASEYRRTFDDVTISLCESEGVTVGISRATASPATGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV52</accession>
    <name>A0A1U7EV52_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Homolog to cytochrome c-type biogenesis protein CcdA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1608A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322529.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV52"/>
    <dbReference type="STRING" id="348780.NP_1608A"/>
    <dbReference type="EnsemblBacteria" id="CAI48895">
        <property type="protein sequence ID" value="CAI48895"/>
        <property type="gene ID" value="NP_1608A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701199"/>
    <dbReference type="KEGG" id="nph:NP_1608A"/>
    <dbReference type="eggNOG" id="arCOG04522">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1048085_0_0_2"/>
    <dbReference type="OrthoDB" id="205803at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051790">
        <property type="entry name" value="Cytochrome_c-biogenesis_DsbD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31272:SF9">
        <property type="entry name" value="BLL1027 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31272">
        <property type="entry name" value="CYTOCHROME C-TYPE BIOGENESIS PROTEIN HI_1454-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="200"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="218" mass="22012" checksum="F5CEA42E2E750C0C" modified="2017-05-10" version="1">MAVELYGTIAFAFGAGIVTFFAPCSYALLPGYVGYYVAATEDQSPPMAGVFARGIAAAGGVLATFAILSVAAVVASDHIEQALPAAEWFVGFLLVGFGLAIVTGRTGSLHVSLPQRRATVFGFGLFGAVYALAALACVLPMFLAVTVQSLTFSAVGTAVVLAAYAGGFGVLMVVATVAIAVGHDAVTTRLADRTNALTRLAGAILVVAGVGQLYIAAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV57</accession>
    <name>A0A1U7EV57_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF2249 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1548A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322499.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV57"/>
    <dbReference type="STRING" id="348780.NP_1548A"/>
    <dbReference type="EnsemblBacteria" id="CAI48865">
        <property type="protein sequence ID" value="CAI48865"/>
        <property type="gene ID" value="NP_1548A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701177"/>
    <dbReference type="KEGG" id="nph:NP_1548A"/>
    <dbReference type="eggNOG" id="arCOG03929">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_174508_0_0_2"/>
    <dbReference type="OrthoDB" id="103554at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018720">
        <property type="entry name" value="DUF2249"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036868">
        <property type="entry name" value="TusA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10006">
        <property type="entry name" value="DUF2249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64307">
        <property type="entry name" value="SirA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2249" evidence="1">
        <location>
            <begin position="27"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="93" mass="10591" checksum="F408333481F4C379" modified="2017-05-10" version="1">MSESESFPVETWLDQLGGPVDVPMSFLDARELPPPEPLSQTLERLPELDDETVFVQLNDREPQFLYPKLDERGYTYETMSVDDGVVTAIWLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV84</accession>
    <name>A0A1U7EV84_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 10 11">Adenylosuccinate lyase</fullName>
            <shortName evidence="11">ASL</shortName>
            <ecNumber evidence="5 10 11">4.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 11">Adenylosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">purB</name>
        <name evidence="14" type="ordered locus">NP_1658A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + fumarate</text>
            <dbReference type="Rhea" id="RHEA:23920"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N(6)-(1,2-dicarboxyethyl)-AMP = fumarate + AMP</text>
            <dbReference type="Rhea" id="RHEA:16853"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer. Residues from neighboring subunits contribute catalytic and substrate-binding residues to each active site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.2" evidence="5 10 11"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322553.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV84"/>
    <dbReference type="STRING" id="348780.NP_1658A"/>
    <dbReference type="EnsemblBacteria" id="CAI48920">
        <property type="protein sequence ID" value="CAI48920"/>
        <property type="gene ID" value="NP_1658A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703067"/>
    <dbReference type="KEGG" id="nph:NP_1658A"/>
    <dbReference type="eggNOG" id="arCOG01747">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025566_2_0_2"/>
    <dbReference type="OrthoDB" id="7033at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00132"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00336"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070626">
        <property type="term" value="F:(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004018">
        <property type="term" value="F:N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.40.30">
        <property type="entry name" value="Fumarase/aspartase (C-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004769">
        <property type="entry name" value="Pur_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047136">
        <property type="entry name" value="PurB_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013539">
        <property type="entry name" value="PurB_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006764">
        <property type="entry name" value="PRK09285.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00928">
        <property type="entry name" value="purB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43411">
        <property type="entry name" value="ADENYLOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43411:SF1">
        <property type="entry name" value="ADENYLOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08328">
        <property type="entry name" value="ASL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 11" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarate lyase N-terminal" evidence="12">
        <location>
            <begin position="20"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Adenylosuccinate lyase PurB C-terminal" evidence="13">
        <location>
            <begin position="336"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="NCBIfam" id="TIGR00928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAI48920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="462" mass="51073" checksum="41E3280E7371F9B2" modified="2017-05-10" version="1">MTERGPLYAVSPLDGRYARYTEPLTDYASEAALMCARVEVEVEYLIALADLDATPLSLTETTRENLRDCYREFDAEDADVIKQLETSGYGDYEATNHDVKAIEYFIRMYLPDEDEQGPWVHFGLTSEDVNNLAHRLLVKPAVEDVLVPEIRSIRDALADSAREHRDVPMLARTHGQPATPTTYGKELAVYAARLGQALGRLEAAADGITGKLAGASGTYAAHEAAYPDVDWQAVSRSFVESLGLEWRALSTQVNPCDDLAAVFDALRGVNNVLRDLDLDMWLYVSDRYLGQKSVAGETGSSTMPHKVNPIDFENSEGNLTKANSDLTFLADYVTTSRLQRDLSDSTVKRNIGSAFAYCLIAYEKTQRGLSKVTPNEAVMQESLDDNPEVIGEAVQTILRREGDTEAYERIKALTRGQRATLADFRELFDDLDVDESVKAELRALSPAGYTGIGAELVDELDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVA0</accession>
    <name>A0A1U7EVA0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Transcription initiation factor IIB</fullName>
            <shortName evidence="10">TFIIB</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">tfbA1</name>
        <name evidence="10" type="synonym">tfb</name>
        <name evidence="14" type="ordered locus">NP_1684A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre-initiation complex (DNA-TBP-TFIIB).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the TFIIB family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322566.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVA0"/>
    <dbReference type="STRING" id="348780.NP_1684A"/>
    <dbReference type="EnsemblBacteria" id="CAI48933">
        <property type="protein sequence ID" value="CAI48933"/>
        <property type="gene ID" value="NP_1684A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703233"/>
    <dbReference type="KEGG" id="nph:NP_1684A"/>
    <dbReference type="eggNOG" id="arCOG01981">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043736_0_0_2"/>
    <dbReference type="OrthoDB" id="7429at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20549">
        <property type="entry name" value="CYCLIN_TFIIB_archaea_like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20550">
        <property type="entry name" value="CYCLIN_TFIIB_archaea_like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000023">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.170:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00383">
        <property type="entry name" value="TF2B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023484">
        <property type="entry name" value="TFIIB_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023486">
        <property type="entry name" value="TFIIB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001658">
        <property type="entry name" value="PRK00423.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF13">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="Zn_Ribbon_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00782">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51134">
        <property type="entry name" value="ZF_TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIB-type" evidence="13">
        <location>
            <begin position="23"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="repeat" description="1" evidence="10">
        <location>
            <begin position="142"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="repeat" description="2" evidence="10">
        <location>
            <begin position="236"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00053882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAI48933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="322" mass="35964" checksum="0AAB43C246CE3234" modified="2017-05-10" version="1">MPGPTRQRERATEGEAEAETEAEQETGCPECDSDSLVRSGDGGGELVCEDCGLVIEEENIDRGPEWRAFNHSERQSKSRVGAPTTQTMHDKGLTTQIDWKDKDAYGRSISSEKRSQMHRLRKWQERIRTKDAGERNLQFALSEIDRMSSALGVPRSVREVASVIYRRALDDDLIRGRSIEGVATAALYAACRQEGIPRSLDEVADVARVEQKEIGRTYRYIAQELSLGLEPVDPVQYVPRFCSELGLSEEVEQKTREIIEVTAEKGMLSGKSPTGYAAAAIYAASLLCNEKKTQREVAEVAQVTEVTIRNRYQEQIEALGIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVI8</accession>
    <name>A0A1U7EVI8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UspA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_1892A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322669.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVI8"/>
    <dbReference type="STRING" id="348780.NP_1892A"/>
    <dbReference type="EnsemblBacteria" id="CAI49037">
        <property type="protein sequence ID" value="CAI49037"/>
        <property type="gene ID" value="NP_1892A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701294"/>
    <dbReference type="KEGG" id="nph:NP_1892A"/>
    <dbReference type="eggNOG" id="arCOG00449">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_2_1_2"/>
    <dbReference type="OrthoDB" id="14880at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF24">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="2"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="146"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="273" mass="27881" checksum="B388321A52E48290" modified="2017-05-10" version="1">MHVLVPLDGNAPAEAALEYALAAFPDAAVTAVCVVGVVDSAAAADQLPEDRRAEYDRAVADAEAVLDDAAARADGRQLTTEVIYGPPSEAIPDYAADVNADRIVMGEHGQAGASDILLGSVAENVVRNAPVPVTVVRGADAAGVEQQILVGVDGSPLSTAALREAVTAHDPDRVRVLTVVESDGDGPDDNAAAERLLETTIGAVDTEAADIETVVRDGDPADALVDAAATADHVVVGSHGRTGIDRLTLGSVAEAVVRNAPVTVTVVHERQVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVS6</accession>
    <name>A0A1U7EVS6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">IS1341-type transposase ISNph25</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2130A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322784.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVS6"/>
    <dbReference type="STRING" id="348780.NP_2130A"/>
    <dbReference type="EnsemblBacteria" id="CAI49156">
        <property type="protein sequence ID" value="CAI49156"/>
        <property type="gene ID" value="NP_2130A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701359"/>
    <dbReference type="KEGG" id="nph:NP_2130A"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_3_3_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF26">
        <property type="entry name" value="TRANSPOSASE, PROBABLY IS605-TNPB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas12f1-like TNB" evidence="2">
        <location>
            <begin position="305"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="430" mass="48469" checksum="65641933135268DE" modified="2017-05-10" version="1">MAWEVTRTVRVRLDVPDDRKSDLHATNSKFQYCANRTADWAWRYPDESCVTSKSEAEAAIYDDLREETDYLHANLVQKAIKRATDDIGNCVDRLADGENTSKPHYDTFSIVYDKRAATYYRDKVSLATVNGRVECEYDLSDDPDGTPHGEYLLNDDYSFSTSTVHYDSETDEFYLHAAMERKLDVSSPEKAEHSRVLGVDCNVDDHIAVTSTGAFVGNADYLNHQRREFEKRRASLQQTGTRSAHLTFQRIGDTFGRWSEDYLHQCSKEIVAEAKRHGCTHIAFENLEQIRARISDDKKFQQWAFRALQEQTEHKAEMAGIVVKTVEPSYTSQQCSKCGCTLEENRDGQQFQCLDCSYTANADYNAAKNIARKLALKLQRGQKSPAGGAFCQYALKSGTMTVNATEVASDTYVSAERESTDKPTASAVGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW34</accession>
    <name>A0A1U7EW34_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2402A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322918.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW34"/>
    <dbReference type="STRING" id="348780.NP_2402A"/>
    <dbReference type="EnsemblBacteria" id="CAI49292">
        <property type="protein sequence ID" value="CAI49292"/>
        <property type="gene ID" value="NP_2402A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701436"/>
    <dbReference type="KEGG" id="nph:NP_2402A"/>
    <dbReference type="eggNOG" id="arCOG03888">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071225_0_0_2"/>
    <dbReference type="OrthoDB" id="342245at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="250" mass="27108" checksum="11DB9A08C0CF52CE" modified="2017-05-10" version="1">MKRSKFVALLVAGLLVGTLFVGGAAAATSSSEDLPEDAEVGTDFEANFEVEDLFDEFNTWTLAAETELTDVTWTVWQYDPAGSEIDRSETDGQSAAQDVDIDDNVDRIEVRVTGTVPEIENISYDPPHQFSAANFTQERSGGTDRHINDHGVHHYTEESNEARQALDSAREAVNASGSGQDTFESAVSSYENGNFENAISLAESAESDATRSQLIRTGLLAGGAIIVLALLGFGGYRVYQSRKQDTSRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWA0</accession>
    <name>A0A1U7EWA0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">UPF0361 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">NP_2564A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SOS response-associated peptidase family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322998.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWA0"/>
    <dbReference type="STRING" id="348780.NP_2564A"/>
    <dbReference type="EnsemblBacteria" id="CAI49373">
        <property type="protein sequence ID" value="CAI49373"/>
        <property type="gene ID" value="NP_2564A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701488"/>
    <dbReference type="KEGG" id="nph:NP_2564A"/>
    <dbReference type="eggNOG" id="arCOG02784">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035990_6_2_2"/>
    <dbReference type="OrthoDB" id="109020at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106300">
        <property type="term" value="P:protein-DNA covalent cross-linking repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1680.10">
        <property type="entry name" value="SOS response associated peptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003738">
        <property type="entry name" value="SRAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036590">
        <property type="entry name" value="SRAP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13604:SF0">
        <property type="entry name" value="ABASIC SITE PROCESSING PROTEIN HMCES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13604">
        <property type="entry name" value="DC12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02586">
        <property type="entry name" value="SRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143081">
        <property type="entry name" value="BB1717-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0190">Covalent protein-DNA linkage</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="197"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI49373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="233" mass="25779" checksum="67C27F96057B289F" modified="2017-05-10" version="1">MCGRYTLFTPPDELESRFDATVGFEFEPRYNAAPGQQLPVVVDTDPGRIQRLEWGLVPRWADDDSGGHINARSETVAEKPAFAEAYQRRRCLVLADGFYEWADRGDGKRPYRVAFDDDRPFAMAGVWERWTPETQQVGLDAFGDGATDGGDPEPLETFTILTTEPNGVVEPLHHRMAVILNADDEGAWLNGDSVSLSPASGDNMRITPVSSAVNDPSNDRPGLIKPADTDRQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWA3</accession>
    <name>A0A1U7EWA3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Probable 2-oxoacid dehydrogenase E1 component alpha subunit</fullName>
            <ecNumber evidence="3">1.2.4.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">oxdhA2</name>
        <name evidence="3" type="ordered locus">NP_2542A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.4.-" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322987.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWA3"/>
    <dbReference type="STRING" id="348780.NP_2542A"/>
    <dbReference type="EnsemblBacteria" id="CAI49362">
        <property type="protein sequence ID" value="CAI49362"/>
        <property type="gene ID" value="NP_2542A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703004"/>
    <dbReference type="KEGG" id="nph:NP_2542A"/>
    <dbReference type="eggNOG" id="arCOG01054">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029393_1_0_2"/>
    <dbReference type="OrthoDB" id="25266at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016624">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009083">
        <property type="term" value="P:branched-chain amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02000">
        <property type="entry name" value="TPP_E1_PDC_ADC_BCADC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050771">
        <property type="entry name" value="Alpha-ketoacid_DH_E1_comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43380">
        <property type="entry name" value="2-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43380:SF1">
        <property type="entry name" value="2-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dehydrogenase E1 component" evidence="2">
        <location>
            <begin position="46"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="379" mass="41995" checksum="D0E8DB3BFC9AE21F" modified="2017-05-10" version="1">MDTADAIDAFAEGENLHRRLDADGQPLVDGYEPPLSDDRLRELHRDMRLARHFDERMVSLQRQGRIGTYAPLAGQEAAQFGSMYALDDDDWVSYQYREHGAVIDRGGLADYIRYWMGYESGNATLVEHNIAPLNISIAAHVPHAVGMAWAAKLRGDDRAVVCHFGDGATSEGDFHEGANIAGVFDVPAVFVCNNNQWAISVPQAAQTASDTFAAKAEAYGFPGVRVDGMDPLATYEVMDEALDRAKSDDERPTDAGTASRPSLVEAVQYRFGAHTTADDPSAYRTDEAVERWRRWDPLPRFEGFLREFGLLDDEAVSAVESDVEATVADAVDQAMAVEPDPDDLFADAYAELPDDIKSQRAYLQRLREEYGDKALREEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWB6</accession>
    <name>A0A1U7EWB6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Chaperonin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2586A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323009.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWB6"/>
    <dbReference type="STRING" id="348780.NP_2586A"/>
    <dbReference type="EnsemblBacteria" id="CAI49384">
        <property type="protein sequence ID" value="CAI49384"/>
        <property type="gene ID" value="NP_2586A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701498"/>
    <dbReference type="KEGG" id="nph:NP_2586A"/>
    <dbReference type="eggNOG" id="arCOG01506">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_992496_0_0_2"/>
    <dbReference type="OrthoDB" id="320758at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3480.10">
        <property type="entry name" value="TorD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020945">
        <property type="entry name" value="DMSO/NO3_reduct_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036411">
        <property type="entry name" value="TorD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050289">
        <property type="entry name" value="TorD/DmsD_chaperones"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34227:SF11">
        <property type="entry name" value="CHAPERONE PROTEIN TORD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34227">
        <property type="entry name" value="CHAPERONE PROTEIN YCDY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02613">
        <property type="entry name" value="Nitrate_red_del"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89155">
        <property type="entry name" value="TorD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="267"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="312" mass="34499" checksum="A16EAFFE05AE4CCC" modified="2017-05-10" version="1">MSNNILRSRSETTDERRTGPDAGGSSVDRTLLEAGSAVDAAHRSRLYGLVALALDRPGDDHREAMADGAFIEELRESASIYDDKRIEQAIDRVADAYTDLDEHRREWASLFGVEEGVTVSPYQLTYLPGPLVTTVRELADMKGFYQAFGLEIAPEERDRGDNIVFQVEFLSHLLFREATLRQANDTEGVSVVVGAQRSFLEDHLGRWYWRFAEEVAKQDTGGFYVAVAELLAALVEEDIDRLGADPEWVPDDPEVTEWNEGVFGDSGRNCGGCGAADDEGEAPTDAGLMDTYGPPDEDRLPDIDEYGPLNDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWF1</accession>
    <name>A0A1U7EWF1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2644A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323038.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWF1"/>
    <dbReference type="EnsemblBacteria" id="CAI49413">
        <property type="protein sequence ID" value="CAI49413"/>
        <property type="gene ID" value="NP_2644A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701509"/>
    <dbReference type="KEGG" id="nph:NP_2644A"/>
    <dbReference type="eggNOG" id="arCOG07497">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170795_0_1_2"/>
    <dbReference type="OrthoDB" id="242103at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058341">
        <property type="entry name" value="DUF8028"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26071">
        <property type="entry name" value="DUF8028"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="73" mass="7554" checksum="D761C80FDA8E00CB" modified="2017-05-10" version="1">MSPFITDDRLTVAKPPSLPGPLKAGAFWTAVLMPFLALGVLASGLGSPLEYLAFTALLVANVLALVAGHDYGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWL5</accession>
    <name>A0A1U7EWL5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Homolog to endonuclease VapC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">NP_2786A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323109.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWL5"/>
    <dbReference type="STRING" id="348780.NP_2786A"/>
    <dbReference type="EnsemblBacteria" id="CAI49484">
        <property type="protein sequence ID" value="CAI49484"/>
        <property type="gene ID" value="NP_2786A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701558"/>
    <dbReference type="KEGG" id="nph:NP_2786A"/>
    <dbReference type="eggNOG" id="arCOG04502">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136715_3_0_2"/>
    <dbReference type="OrthoDB" id="41298at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000042">
        <property type="entry name" value="Ribonuclease VapC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039018">
        <property type="entry name" value="VapC20-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42188">
        <property type="entry name" value="23S RRNA-SPECIFIC ENDONUCLEASE VAPC20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42188:SF1">
        <property type="entry name" value="23S RRNA-SPECIFIC ENDONUCLEASE VAPC20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="domain" description="PIN" evidence="7">
        <location>
            <begin position="4"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="144" mass="15898" checksum="49EBDEC201E5742F" modified="2017-05-10" version="1">MPRVLIDTTVLFAATYRQDSAHDKALPILSGIDTAALPEVVILDYVLAETLNGLTTHAGHDAAVDFLDRIEENARFHIESLTADAFATAKALFRQYEQFSLVDACIVAYMQNEGFGYLYAFDDDFDAAADISRLTTATNPYQPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWP4</accession>
    <name>A0A1U7EWP4_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">Iron-sulfur cluster carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">apbC1</name>
        <name evidence="10" type="ordered locus">NP_2824A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 8">Binds and transfers iron-sulfur (Fe-S) clusters to target apoproteins. Can hydrolyze ATP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the Mrp/NBP35 ATP-binding proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323128.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWP4"/>
    <dbReference type="STRING" id="348780.NP_2824A"/>
    <dbReference type="EnsemblBacteria" id="CAI49503">
        <property type="protein sequence ID" value="CAI49503"/>
        <property type="gene ID" value="NP_2824A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702936"/>
    <dbReference type="KEGG" id="nph:NP_2824A"/>
    <dbReference type="eggNOG" id="arCOG00585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG01846">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024839_0_0_2"/>
    <dbReference type="OrthoDB" id="8297at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02037">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001119">
        <property type="entry name" value="Iron-sulfur cluster carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000808">
        <property type="entry name" value="Mrp-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044304">
        <property type="entry name" value="NUBPL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42961">
        <property type="entry name" value="IRON-SULFUR PROTEIN NUBPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42961:SF2">
        <property type="entry name" value="IRON-SULFUR PROTEIN NUBPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01215">
        <property type="entry name" value="MRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0408">Iron</keyword>
    <keyword evidence="5 8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIP18 family-like" evidence="9">
        <location>
            <begin position="3"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="99"/>
            <end position="106"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00074706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI49503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="348" mass="37082" checksum="CF9712BF72053BAF" modified="2017-05-10" version="1">MNEETVLDRLAAVEDPDLGDDIVSLGLVNDVNIDAETIHVDLALGAPYSPTETELAGTVRDALSELDREIDLTASVDTGLSADEQILPDVENIIAVASGKGGVGKSTVAVNLAAGLSQLGARVGLFDADVYGPNVPRMVEADDQPKATEQETIIPPEKYGMKLMSMDFLVGEDDPVIWRGPMVHKVLTQLWEDVEWGALDYMVVDLPPGTGDTQLTLLQSVPVSGAVIVTTPQKVALDDAEKGLQMFGEHDTPVLGIVENMSGFVCPDCGSEHDIFGSGGGESFADDVEMPFLGRIPLDPAVREGGDAGRPVVLDEDDETGEALRSFTERTANMQGIVRRRQVSAADR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWQ7</accession>
    <name>A0A1U7EWQ7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2898A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323165.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWQ7"/>
    <dbReference type="STRING" id="348780.NP_2898A"/>
    <dbReference type="EnsemblBacteria" id="CAI49540">
        <property type="protein sequence ID" value="CAI49540"/>
        <property type="gene ID" value="NP_2898A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701588"/>
    <dbReference type="KEGG" id="nph:NP_2898A"/>
    <dbReference type="eggNOG" id="arCOG09325">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_205632_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="66" mass="7054" checksum="5F44EE77630944E2" modified="2017-05-10" version="1">MFPKVRSALRSAWPILGSLYLLYLALQQPPVRYVGIGGLVVVTPLLVGWAAGTLFGVGPWANDDDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWT3</accession>
    <name>A0A1U7EWT3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2950A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323190.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWT3"/>
    <dbReference type="STRING" id="348780.NP_2950A"/>
    <dbReference type="EnsemblBacteria" id="CAI49566">
        <property type="protein sequence ID" value="CAI49566"/>
        <property type="gene ID" value="NP_2950A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701609"/>
    <dbReference type="KEGG" id="nph:NP_2950A"/>
    <dbReference type="eggNOG" id="arCOG06270">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034656_1_0_2"/>
    <dbReference type="OrthoDB" id="145655at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.210.10">
        <property type="entry name" value="Cytochrome c oxidase-like, subunit I domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036927">
        <property type="entry name" value="Cyt_c_oxase-like_su1_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81442">
        <property type="entry name" value="Cytochrome c oxidase subunit I-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="257"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="287"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="321"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="372"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="401"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="448" mass="46928" checksum="44413AC958B8468C" modified="2017-05-10" version="1">MAAIPDAIDADRGPPMAVPLQHFVVALVFLLAGTVAGAAGQFGLLAGMEHVAFVHLLFVGWVCITILGAMTQFVPVWSGLDLHSRRLANVQLLFVTVGLAGLVAALFSGAVAWLAATGTVLAVGIWLFVYNIGRTLAAARPWDVTERHFAFALGCFVAVTMLALPLAVGFSRPVFTSLPVTHESVRMAHASVAVFGAVLATVLGALYQLATMSTQTELHGIDTTVRRAETVAYPVGVTALVVGRLFETVLLARLGGVLVAGCIIAVGFVVARRLYETQVDQTPMLSRYAVVAVAMVIWGLLALPAWAVAPLAAGTLYGAEGTFYLLAFGVVGFVVFGTLYHIVPFLIWVDRYSGQLGLADVPMIDDLYSDRLAVVDFTLLSGGVVALVIADSLAVPSSVRFFGSLLVLAGGAVFVANITLVVRRHNPGGVRGLVSDTGSAAGSPEQSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX08</accession>
    <name>A0A1U7EX08_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NosL family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">nosL2</name>
        <name evidence="2" type="ordered locus">NP_3142A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323284.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX08"/>
    <dbReference type="STRING" id="348780.NP_3142A"/>
    <dbReference type="EnsemblBacteria" id="CAI49662">
        <property type="protein sequence ID" value="CAI49662"/>
        <property type="gene ID" value="NP_3142A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701666"/>
    <dbReference type="KEGG" id="nph:NP_3142A"/>
    <dbReference type="eggNOG" id="arCOG04012">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1346437_0_0_2"/>
    <dbReference type="OrthoDB" id="241788at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008719">
        <property type="entry name" value="N2O_reductase_NosL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41247">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REPRESSOR YCNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41247:SF1">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REPRESSOR YCNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05573">
        <property type="entry name" value="NosL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160387">
        <property type="entry name" value="NosL/MerB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="226" mass="25498" checksum="8F5F808F15FD2141" modified="2017-05-10" version="1">MSKQTQDTWTRRRMLAVAGAGAVVGLAGCADESSEEPEEEPEEAPEEDPEEDPEEEPEALAHDEPAEFPEGEECAVCNMVTPEYPEWNAQVVHENGDRAYFCSSGCLSAYVADPEHFDGPGSDIENAWVTCYESGELIDGKEAYYVRVTDSDHVDDPMMRNPTPFGMREDAEAFADEFDEYDDSDIITYGDFDQDLAEFYRGNFFADEDMDDHDHDDMDHDHDDME</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX36</accession>
    <name>A0A1U7EX36_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Ion channel pore / TrkA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">pchA1</name>
        <name evidence="10" type="ordered locus">NP_3178A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323302.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX36"/>
    <dbReference type="STRING" id="348780.NP_3178A"/>
    <dbReference type="EnsemblBacteria" id="CAI49680">
        <property type="protein sequence ID" value="CAI49680"/>
        <property type="gene ID" value="NP_3178A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701678"/>
    <dbReference type="KEGG" id="nph:NP_3178A"/>
    <dbReference type="eggNOG" id="arCOG01958">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057267_1_0_2"/>
    <dbReference type="OrthoDB" id="56871at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005267">
        <property type="term" value="F:potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003280">
        <property type="entry name" value="2pore_dom_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050721">
        <property type="entry name" value="Trk_Ktr_HKT_K-transport"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43833">
        <property type="entry name" value="POTASSIUM CHANNEL PROTEIN 2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43833:SF9">
        <property type="entry name" value="POTASSIUM CHANNEL PROTEIN YUGO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01333">
        <property type="entry name" value="2POREKCHANEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="88"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="202"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="9">
        <location>
            <begin position="247"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI49680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="394" mass="41957" checksum="173F297A30507A4B" modified="2017-05-10" version="1">MTDTGERDGSRAVRSVRWLGSRLPFLLTAIVAVLSVVTGIVNIGATGVSGPFAAYIPEAMARTAGFTGALTGFLMLLSAYGLRRCYRIAWYSTLVLLPITALQGLAQSSVVSYPLVGLSAISLPVVLLNYSYFDRAANLTTTQLASLAAVVFVQVYGTIGAYALQDEFEGINTILDAFYFTLVTASTVGYGDITATTQFGRLFSMSVLLTGVSSFGVAVGTLLGPLIEARLATALGNMSDKQLDLLDDHFIVIGYGDLTEPILESLGTASAVVVVPDPEDAKRLRDRDYEVITADPSDEDPLQRVHIERAKGLIAATNDDAQDAMTVLTARELNPELRIVAAATDRENVRKLRRAGADTVLSPSVLGGRLLVESAFGNDDPEQVLNELLGVGKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX44</accession>
    <name>A0A1U7EX44_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sulfurtransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">tssA</name>
        <name evidence="5" type="ordered locus">NP_3186A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323306.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX44"/>
    <dbReference type="STRING" id="348780.NP_3186A"/>
    <dbReference type="EnsemblBacteria" id="CAI49684">
        <property type="protein sequence ID" value="CAI49684"/>
        <property type="gene ID" value="NP_3186A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702649"/>
    <dbReference type="KEGG" id="nph:NP_3186A"/>
    <dbReference type="eggNOG" id="arCOG02019">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031618_1_3_2"/>
    <dbReference type="OrthoDB" id="10492at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01448">
        <property type="entry name" value="TST_Repeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01449">
        <property type="entry name" value="TST_Repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051126">
        <property type="entry name" value="Thiosulfate_sulfurtransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001307">
        <property type="entry name" value="Thiosulphate_STrfase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43855">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43855:SF1">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00380">
        <property type="entry name" value="RHODANESE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00683">
        <property type="entry name" value="RHODANESE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rhodanese" evidence="4">
        <location>
            <begin position="25"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="4">
        <location>
            <begin position="171"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="200"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="295" mass="33218" checksum="F8DECE36D59CBC90" modified="2017-05-10" version="1">MTEHDSPTLVSADWVEEHLEDFQSDDPEYRLLEVNNPTVTADSEYTPYEEGHIPGAIFFDWDDDLSDETTRDILDKDAFEELNAEAGITEESTIVLYGGGRVPNWFALFAYWQYRYFGHDDVRIIDGGKPYWVENDYPLTADEQAFAAQEYEASGPYESIRAYKSDVEQAIDSGIPLVDVRSPEEFTGEVIAPEGLQETAQRGGHIPGAKSVPTTSVLDDDGTFKGPDELRELYEDVGITEDQSVITYCRVGERSSIEWYVLAELLGFDDVQNYDGSWTEWGNTIRAPIETGDAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX76</accession>
    <name>A0A1U7EX76_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 20">Inosine-5'-monophosphate dehydrogenase</fullName>
            <shortName evidence="13">IMP dehydrogenase</shortName>
            <shortName evidence="13">IMPD</shortName>
            <shortName evidence="13">IMPDH</shortName>
            <ecNumber evidence="13 20">1.1.1.205</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">guaB1</name>
        <name evidence="13" type="synonym">guaB</name>
        <name evidence="23" type="ordered locus">NP_3384A</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13 20">
            <text>IMP + NAD(+) + H2O = XMP + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="1.1.1.205"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="13">Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.</text>
    </comment>
    <comment type="pathway">
        <text evidence="13 20">Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 13">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13 19">Belongs to the IMPDH/GMPR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.205" evidence="13 20"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="RefSeq" id="WP_011323403.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A1U7EX76"/>
    <dbReference type="STRING" id="348780.NP_3384A"/>
    <dbReference type="EnsemblBacteria" id="CAI49783">
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    <dbReference type="GeneID" id="3702784"/>
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        <property type="reaction ID" value="UER00295"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="term" value="F:nucleotide binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Aldolase_TIM"/>
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        <property type="entry name" value="IMP_DH"/>
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        <property type="entry name" value="INOSINE-5'-MONOPHOSPHATE DEHYDROGENASE"/>
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    </dbReference>
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        <property type="entry name" value="INOSINE-5-MONOPHOSPHATE DEHYDROGENASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF00478">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51412">
        <property type="entry name" value="Inosine monophosphate dehydrogenase (IMPDH)"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="IMP_DH_GMP_RED"/>
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    </dbReference>
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    <keyword evidence="11 18" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6 13 20" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="4 13 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13 16" id="KW-0520">NAD</keyword>
    <keyword evidence="9 13 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 13 17" id="KW-0630">Potassium</keyword>
    <keyword evidence="7 13 20" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="22">
        <location>
            <begin position="101"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="22">
        <location>
            <begin position="163"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="468"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="active site" description="Thioimidate intermediate" evidence="13 14">
        <location>
            <position position="309"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13 14">
        <location>
            <position position="405"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="254"/>
            <end position="256"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 16">
        <location>
            <begin position="302"/>
            <end position="304"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13 17">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13 17">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
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        </ligand>
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    <feature type="binding site" evidence="13 15">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
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    <feature type="binding site" description="in other chain" evidence="13 17">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
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    <feature type="binding site" evidence="13 15">
        <location>
            <begin position="342"/>
            <end position="344"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
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        <location>
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            <end position="366"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
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        <location>
            <begin position="389"/>
            <end position="393"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
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        <location>
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        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
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        <location>
            <position position="473"/>
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        <ligand>
            <name>K(+)</name>
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        </location>
        <ligand>
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        <ligand>
            <name>K(+)</name>
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    </evidence>
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        <source>
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    </evidence>
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    <sequence length="493" mass="52618" checksum="D271EC3A59920317" modified="2017-05-10" version="1">MASDEPFSEKLRVPEALTFDDVLLRPKESRVEPDDADMATRVSTNVSLNIPVISAAMDTVTEAELAIEMARQGGLGVLHRNMSVEETAQHVEEVKRADELIIRDVVTASPDQTVREVDAMMERKGVSGAPVVDDDDTVLGIISGTDIRPYLEVGEHDSVQEAMTDEVITATEEVSPREALELMYEHKIERVPIVDDENRLTGLVTMAGVLARREYDNAARDDDGALVVGVAVGPFELERARAADAAGADIVFIDCAHAHNLDVIDSAREIREEVDADVVVGNIGTREAAEAVVDFADGVKVGIGPGSICTTRVVSGSGMPQITAVAEVADVASQHDTPVIADGGIRYSGDAIKAIAAGADAVMLGSYFAGTEEAPGRVITMNGKRYKQYRGMGSVGAMNDGGGDRYLKDDEEDEEFVPEGVEAATPYKGPLSSELHQLVGGMQSGMGYVGAETIPEFKERSEFVRVSSAGQTESHAHDVVITDEAPNYSPDSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX93</accession>
    <name>A0A1U7EX93_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3364A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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        <property type="gene ID" value="NP_3364A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3364A"/>
    <dbReference type="eggNOG" id="arCOG04575">
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        <property type="entry name" value="DUF5789"/>
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    <dbReference type="Pfam" id="PF19102">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="109" mass="12321" checksum="0C53AA1D779821F7" modified="2017-05-10" version="1">MGVRPPQANDDEEPEIIAFGIAALDEYLEQGNVTFPATDEELRRALGDPDIPYDAKGHTISFGSALERAGRERFENRQQFMNALHPVFEAERERTNNSFVGRVRSLLPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXA1</accession>
    <name>A0A1U7EXA1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">AhpD family alkylhydroperoxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3402A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323412.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXA1"/>
    <dbReference type="STRING" id="348780.NP_3402A"/>
    <dbReference type="EnsemblBacteria" id="CAI49792">
        <property type="protein sequence ID" value="CAI49792"/>
        <property type="gene ID" value="NP_3402A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701765"/>
    <dbReference type="KEGG" id="nph:NP_3402A"/>
    <dbReference type="eggNOG" id="arCOG06151">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082760_4_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.810">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004675">
        <property type="entry name" value="AhpD_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003779">
        <property type="entry name" value="CMD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010195">
        <property type="entry name" value="Uncharacterised_peroxidase-rel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00778">
        <property type="entry name" value="ahpD_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01926">
        <property type="entry name" value="peroxid_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35446:SF2">
        <property type="entry name" value="CARBOXYMUCONOLACTONE DECARBOXYLASE-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35446">
        <property type="entry name" value="SI:CH211-175M2.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02627">
        <property type="entry name" value="CMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carboxymuconolactone decarboxylase-like" evidence="1">
        <location>
            <begin position="46"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="188" mass="21188" checksum="2CB7266B34CF579C" modified="2017-05-10" version="1">MTDDAMGNFPVPELSDLPEDIKERIESETERAGFTPNVFSAFAYKPSHFRAFFEYHDAIVDESPLEREEIEMLIVTVSGVNDCLYCVVAHGALLRVYADAPKLAEQLATNHRSADLSEPHRAMLDFAVTLTETPGKVDERDLERLREAGFSTEEIWDIGSVVALFNLSNRMATLADIRPNEEFYTLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXC3</accession>
    <name>A0A1U7EXC3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable PrkA-type serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">prkA2</name>
        <name evidence="2" type="ordered locus">NP_3446A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323434.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXC3"/>
    <dbReference type="STRING" id="348780.NP_3446A"/>
    <dbReference type="EnsemblBacteria" id="CAI49814">
        <property type="protein sequence ID" value="CAI49814"/>
        <property type="gene ID" value="NP_3446A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703053"/>
    <dbReference type="KEGG" id="nph:NP_3446A"/>
    <dbReference type="eggNOG" id="arCOG02892">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_361184_0_0_2"/>
    <dbReference type="OrthoDB" id="296642at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013153">
        <property type="entry name" value="Prk_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010650">
        <property type="entry name" value="PrkA_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30267">
        <property type="entry name" value="PROTEIN KINASE PRKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30267:SF2">
        <property type="entry name" value="PROTEIN PRKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08298">
        <property type="entry name" value="AAA_PrkA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06798">
        <property type="entry name" value="PrkA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00763">
        <property type="entry name" value="AAA_PrkA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PrkA AAA" evidence="1">
        <location>
            <begin position="22"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="758" mass="86054" checksum="222235B7C6C53095" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXD7</accession>
    <name>A0A1U7EXD7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF1918 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3526A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323474.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXD7"/>
    <dbReference type="STRING" id="348780.NP_3526A"/>
    <dbReference type="EnsemblBacteria" id="CAI49854">
        <property type="protein sequence ID" value="CAI49854"/>
        <property type="gene ID" value="NP_3526A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701795"/>
    <dbReference type="KEGG" id="nph:NP_3526A"/>
    <dbReference type="eggNOG" id="arCOG04639">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2766212_0_0_2"/>
    <dbReference type="OrthoDB" id="241713at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015035">
        <property type="entry name" value="DUF1918"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08940">
        <property type="entry name" value="DUF1918"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1918" evidence="1">
        <location>
            <begin position="3"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="62" mass="6847" checksum="1617D30A17DA0035" modified="2017-05-10" version="1">MAFEKGDRVVFHDKHSDYDGETGEVAQVSETMFGDKTYVVDFEEGQEGGIAEDALEAAEDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXE4</accession>
    <name>A0A1U7EXE4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">GATase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3486A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323454.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXE4"/>
    <dbReference type="STRING" id="348780.NP_3486A"/>
    <dbReference type="EnsemblBacteria" id="CAI49834">
        <property type="protein sequence ID" value="CAI49834"/>
        <property type="gene ID" value="NP_3486A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701786"/>
    <dbReference type="KEGG" id="nph:NP_3486A"/>
    <dbReference type="eggNOG" id="arCOG01312">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065735_1_0_2"/>
    <dbReference type="OrthoDB" id="239338at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025646">
        <property type="entry name" value="DUF4350"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14258">
        <property type="entry name" value="DUF4350"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4350" evidence="2">
        <location>
            <begin position="115"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="243"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="328"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="340" mass="34823" checksum="552693C39B99DB21" modified="2017-05-10" version="1">MSSDSRSLVGVFVLVFAVTVGAVVVGSIGLDMVGEQPEVEEDHWNLDAVAPEGAETGGEIAMDSFEAENTVVVHVPPSVPAGGGIAMPIEDDTDLDEASVGSIGGAERDISPLASTLAENGHEVVFYQDEFEDGPLDETLAEADGFISTGVGMLSGEETDAVEEFADSGGRVVLTANPGSAGPATDVGSSFGLYADFGYVYDMAENDHNYLSVFVEPADGSPLTDGVDRAVFRGAAPVGASDGSGTFETSDSAQISTTRETGSYTVAATSGDVALVGDSSFMSPENAKRADNNVLVGNVADYLVTGGPVTMDLDDDDEPEPGPAPPGDPDDDVDVEPIQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXE9</accession>
    <name>A0A1U7EXE9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Signal peptide peptidase SppA</fullName>
            <ecNumber evidence="7">3.4.21.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">sppA2</name>
        <name evidence="7" type="ordered locus">NP_3488A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S49 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323455.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXE9"/>
    <dbReference type="STRING" id="348780.NP_3488A"/>
    <dbReference type="EnsemblBacteria" id="CAI49835">
        <property type="protein sequence ID" value="CAI49835"/>
        <property type="gene ID" value="NP_3488A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703215"/>
    <dbReference type="KEGG" id="nph:NP_3488A"/>
    <dbReference type="eggNOG" id="arCOG01311">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076515_0_0_2"/>
    <dbReference type="OrthoDB" id="27099at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07023">
        <property type="entry name" value="S49_Sppa_N_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002142">
        <property type="entry name" value="Peptidase_S49"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047272">
        <property type="entry name" value="S49_SppA_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42987">
        <property type="entry name" value="PEPTIDASE S49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42987:SF4">
        <property type="entry name" value="PROTEASE SOHB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01343">
        <property type="entry name" value="Peptidase_S49"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S49" evidence="6">
        <location>
            <begin position="102"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S49" evidence="6">
        <location>
            <begin position="166"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="141"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="290"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="148"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="160"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="315" mass="32877" checksum="870DB15DAE97A850" modified="2017-05-10" version="1">MPIGPLNKLSGRQQVALVVLLAIAAGVLAAPQVYSFVADDGDTVVVIEMQGPIETNMAQDVEDQLRDARQDDAVEAVVLEVESGGGLPAQSERIYAAVDRTSEEMPVIATVDTMGASGAYLSMAPADEIYVAPSAQAVGSVGVAGTAPSPSGPNAGTTGPDKRGPSTEQQREQQQILANLFVESVIEQRGDEIELDREEIAHANSYLGTEAVNNGYADDFGFVDDAIEDAADEAGLRTYSVETHRADGVGVGMPLIEDDGDIVVTENDETLNDAFILAVAPEVWEETVGTELEPVSYTGPEPSEVGADADGGVDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXG3</accession>
    <name>A0A1U7EXG3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Oxidoreductase (Homolog to thioredoxin-disulfide reductase)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3544A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323483.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXG3"/>
    <dbReference type="STRING" id="348780.NP_3544A"/>
    <dbReference type="EnsemblBacteria" id="CAI49863">
        <property type="protein sequence ID" value="CAI49863"/>
        <property type="gene ID" value="NP_3544A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701801"/>
    <dbReference type="KEGG" id="nph:NP_3544A"/>
    <dbReference type="eggNOG" id="arCOG01296">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037483_0_0_2"/>
    <dbReference type="OrthoDB" id="213162at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050982">
        <property type="entry name" value="Auxin_biosynth/cation_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43539">
        <property type="entry name" value="FLAVIN-BINDING MONOOXYGENASE-LIKE PROTEIN (AFU_ORTHOLOGUE AFUA_4G09220)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43539:SF89">
        <property type="entry name" value="NAD(P)-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13738">
        <property type="entry name" value="Pyr_redox_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="287"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="421" mass="44630" checksum="5C130AA1B4935ED5" modified="2017-05-10" version="1">MTLPDSVDVAVVGAGPAGVGIGTALSFLDVETVILERDAVGASFRNWPAEMRFITPSFPSNGFGHPDLNAVVPATSPGAILDTQQPSGDEYAEYLQRAVDHYELPVETGIEVTTVTPLSDAAPESGGPAVSEPRGVAVDGGDTEGFRLETSGGSVHAQCVVWAGGQFGTPRTDVFSGAALCVHNAAVDSWADHAAANSTDEFLVVGGFESGIDAAVNLVELGCSVTVLDRGHPWATRHPDPSQTLSPYTLQRLDEVRDSEKLTLVGGAEVQEVHRTDDGQFAVHARPVGSDPVSGAPPTANAGWEDHSDGTFVVPTRPVLATGFDSDFGPVEELFPRERGSVRLTERDESPTTPGLFLTGPEVEHNGQKFCFIYKFRARLPVVAEEIGERLGVATEPLDQYREAGMFLDDLDCCEPVDCNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXH8</accession>
    <name>A0A1U7EXH8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3598A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323510.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXH8"/>
    <dbReference type="STRING" id="348780.NP_3598A"/>
    <dbReference type="EnsemblBacteria" id="CAI49890">
        <property type="protein sequence ID" value="CAI49890"/>
        <property type="gene ID" value="NP_3598A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701819"/>
    <dbReference type="KEGG" id="nph:NP_3598A"/>
    <dbReference type="eggNOG" id="arCOG03916">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_179006_0_0_2"/>
    <dbReference type="OrthoDB" id="297458at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055943">
        <property type="entry name" value="DUF7521"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24365">
        <property type="entry name" value="DUF7521"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="100" mass="10920" checksum="CD24473483345CD0" modified="2017-05-10" version="1">MIDVEVWMFVASNLLVFAVGGTLTVLSYRAQRRFEQTSLRYTTLGFALITVSTVVEAFYAPGMINTGPLTDSQLLVLYTVESGLIGLGLASVAYSVLQYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXI0</accession>
    <name>A0A1U7EXI0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC-type transport system permease protein (Probable substrate dipeptide/oligopeptide)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">dppC2</name>
        <name evidence="10" type="synonym">abc02p2</name>
        <name evidence="10" type="ordered locus">NP_3582A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family. OppBC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323502.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXI0"/>
    <dbReference type="STRING" id="348780.NP_3582A"/>
    <dbReference type="EnsemblBacteria" id="CAI49882">
        <property type="protein sequence ID" value="CAI49882"/>
        <property type="gene ID" value="NP_3582A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702369"/>
    <dbReference type="KEGG" id="nph:NP_3582A"/>
    <dbReference type="eggNOG" id="arCOG00748">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028518_1_2_2"/>
    <dbReference type="OrthoDB" id="312811at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053385">
        <property type="entry name" value="ABC_transport_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050366">
        <property type="entry name" value="BP-dependent_transpt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025966">
        <property type="entry name" value="OppC_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045474">
        <property type="entry name" value="Opp2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43386:SF1">
        <property type="entry name" value="D,D-DIPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN DDPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43386">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN APPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12911">
        <property type="entry name" value="OppC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="94"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="157"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="261"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="92"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI49882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="300" mass="30832" checksum="2820640CD73CCE90" modified="2017-05-10" version="1">MSSATTGDHNGTVGAGAPLSRRLRLALRDTQTAVGATIVGILAIVAIVGPFVVPHDPVAQDLTRPLQGPSLAHPFGTDPLGRDMLARIVYGARLSLAIAFAVTLVRLVVGILVGVVAGYVGGWVDESLMRLVDLLFAFPGIVLALVIAGIAGPSLRNVMLALAVVGWGTYARVVRSSVLSAREREYVAASRLAGTGHGRVVADHVLPNVLGPVIVLATLDTGKVVLATAGLSFLGLGAQPPTPEWGTMIATGRDYLQTASWLVNIPGLAIAATVFGFTLLGDGLRDALDPTGATEQGCDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXT3</accession>
    <name>A0A1U7EXT3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Probable phosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3880A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323648.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXT3"/>
    <dbReference type="STRING" id="348780.NP_3880A"/>
    <dbReference type="EnsemblBacteria" id="CAI50031">
        <property type="protein sequence ID" value="CAI50031"/>
        <property type="gene ID" value="NP_3880A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701900"/>
    <dbReference type="KEGG" id="nph:NP_3880A"/>
    <dbReference type="eggNOG" id="arCOG00397">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026621_4_0_2"/>
    <dbReference type="OrthoDB" id="11638at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00840">
        <property type="entry name" value="MPP_Mre11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050535">
        <property type="entry name" value="DNA_Repair-Maintenance_Comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041796">
        <property type="entry name" value="Mre11_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30337">
        <property type="entry name" value="COMPONENT OF ATP-DEPENDENT DSDNA EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30337:SF7">
        <property type="entry name" value="PHOSPHOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="2">
        <location>
            <begin position="4"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="441" mass="50593" checksum="B50277FF5F55A6DF" modified="2017-05-10" version="1">MTVRFLHTADLHLGSQLKTQHRQATGTIETLDSAIYTAVERLFDTAIEEDVDFVVIAGDLYDEDSRSVKANTFLKEQFDRLADQNIPAYVSYGNHDPVGSATTYVDLPDNVYEFDHEDPQEFYYPDEDTPEARIWGQSYRDRHESRSMYHRFTPADERVPNIGVLHTGLNPDGRRYVPVARSDLESKDEIHYWALGHIHDPRIYENEQPIAYSGVPQGRQITEPGFGGAYLVELDAEGDYEIEFVPTSPVIWQTVEVDIGDDDISSIPDIERRIEQTLDEFSAPTELFDGTSVAVRDAEWGIDGYVCRWKLTGNGPVHETLSSDDEAIHELTRRLRDGLTSRRPFVWTEAVRDETGPPIPSIDELRGNDRVIDEYIAFIDELDEVDARERYREEVGMVWESVEDHEEGRPDELSLTDEQLDNLIERAQERVLEELARRRAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXU4</accession>
    <name>A0A1U7EXU4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Small CPxCG-related zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3852A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXU4"/>
    <dbReference type="STRING" id="348780.NP_3852A"/>
    <dbReference type="EnsemblBacteria" id="CAI50017">
        <property type="protein sequence ID" value="CAI50017"/>
        <property type="gene ID" value="NP_3852A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3852A"/>
    <dbReference type="eggNOG" id="arCOG04494">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_206264_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057159">
        <property type="entry name" value="DUF7837"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25207">
        <property type="entry name" value="DUF7837"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7837" evidence="1">
        <location>
            <begin position="7"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="50" mass="5235" checksum="59B85106EA426236" modified="2017-05-10" version="1">MSTTGPSTLGGCPFCGADVTSTNVLIEYEVDGQPAVFAECPQCREVVKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXU8</accession>
    <name>A0A1U7EXU8_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="8">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">rad25b</name>
        <name evidence="12" type="ordered locus">NP_3846A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RAD25/XPB subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="8"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323631.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXU8"/>
    <dbReference type="STRING" id="348780.NP_3846A"/>
    <dbReference type="EnsemblBacteria" id="CAI50014">
        <property type="protein sequence ID" value="CAI50014"/>
        <property type="gene ID" value="NP_3846A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701884"/>
    <dbReference type="KEGG" id="nph:NP_3846A"/>
    <dbReference type="eggNOG" id="arCOG00874">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008213_6_0_2"/>
    <dbReference type="OrthoDB" id="11644at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17926">
        <property type="entry name" value="DEXHc_RE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050615">
        <property type="entry name" value="ATP-dep_DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032438">
        <property type="entry name" value="ERCC3_RAD25_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040699">
        <property type="entry name" value="XPB_DRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11274:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION AND DNA REPAIR FACTOR IIH HELICASE SUBUNIT XPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11274">
        <property type="entry name" value="RAD25/XP-B DNA REPAIR HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16203">
        <property type="entry name" value="ERCC3_RAD25_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18458">
        <property type="entry name" value="XPB_DRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="93"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="331"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI50014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="469" mass="53222" checksum="F0BFEAE0CC373FA7" modified="2017-05-10" version="1">MRIAFEDGTLLLENAPDSIPYAEWDDRVDEYRARAQHYREIKEWTSKTDGQATLHESAATVDSVEDVARAYSEFALTPSVAIEPRDYQQKALSAWRANDRRGTVVLPTGSGKTFLAVQAIADAGVSTLVVVPTIDLMNQWHATLTNAFGDQLPDGIGVLGGGSHNVTNITVTTYDSAYRYINEYGDQFGLLVVDEVHHLPAPTYQQIPEMTIAPYRLGLTATYERADGQHEELEDLLGTVVYREEVDELAGDYLSEYETIHLQVELTPDEREEYDEEYQIYRDYVDSHEFDLWKERGYQEFLKRTSYDPQGRRALIAKQRAEEIARTATKKLDTLDNLLKRHYDDRAIIFTANNDFAYDISQEFVVPCITHQTETDERTAILERFRTGEYSMLVTSQVLDEGIDVPAANVGIILSGSASKRQYAQRLGRILRPTDGRQPARLYEIIAEDTVETYVSERRRQGVTSDADS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXV8</accession>
    <name>A0A1U7EXV8_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="9">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">NP_3864A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. UvrD subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="9"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323640.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXV8"/>
    <dbReference type="STRING" id="348780.NP_3864A"/>
    <dbReference type="EnsemblBacteria" id="CAI50023">
        <property type="protein sequence ID" value="CAI50023"/>
        <property type="gene ID" value="NP_3864A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701892"/>
    <dbReference type="KEGG" id="nph:NP_3864A"/>
    <dbReference type="eggNOG" id="arCOG00797">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG00798">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006494_1_1_2"/>
    <dbReference type="OrthoDB" id="203178at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000725">
        <property type="term" value="P:recombinational repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04491">
        <property type="entry name" value="SoSSB_OBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.91.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013986">
        <property type="entry name" value="DExx_box_DNA_helicase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070:SF2">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE SRS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00580">
        <property type="entry name" value="UvrD-helicase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 11 14" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 11 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="13">
        <location>
            <begin position="254"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="179"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="275"/>
            <end position="282"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAI50023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="1028" mass="119824" checksum="8706647CED43BE20" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXX5</accession>
    <name>A0A1U7EXX5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ArsR family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3936A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323676.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXX5"/>
    <dbReference type="STRING" id="348780.NP_3936A"/>
    <dbReference type="EnsemblBacteria" id="CAI50059">
        <property type="protein sequence ID" value="CAI50059"/>
        <property type="gene ID" value="NP_3936A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701916"/>
    <dbReference type="KEGG" id="nph:NP_3936A"/>
    <dbReference type="eggNOG" id="arCOG02611">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109676_1_0_2"/>
    <dbReference type="OrthoDB" id="28610at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056504">
        <property type="entry name" value="HTH_HVO_0163_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36216:SF1">
        <property type="entry name" value="HTH ARSR-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36216">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, TRMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24266">
        <property type="entry name" value="HTH_HVO_0163_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="69"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="169" mass="19374" checksum="6068ACA55C7ADB03" modified="2017-05-10" version="1">MSDTRARVKSHVESNPGVHFNAISRELDIATGQTQYHIRKLLRENRLTSMELCGRTHYFPLGYSEHEQAGFALLRRETTRELVVLLLDEGPMAPDDLATEADIARSTVEWHLSNLVDCELAEKQYEGRSVRVHVTEPELLRELLVEIDPTLPDRLVDRFSRLVDSLLEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXY3</accession>
    <name>A0A1U7EXY3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">NosL family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nosL3</name>
        <name evidence="1" type="ordered locus">NP_3934A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323675.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXY3"/>
    <dbReference type="STRING" id="348780.NP_3934A"/>
    <dbReference type="EnsemblBacteria" id="CAI50058">
        <property type="protein sequence ID" value="CAI50058"/>
        <property type="gene ID" value="NP_3934A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702983"/>
    <dbReference type="KEGG" id="nph:NP_3934A"/>
    <dbReference type="eggNOG" id="arCOG04012">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096026_0_0_2"/>
    <dbReference type="OrthoDB" id="162738at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008719">
        <property type="entry name" value="N2O_reductase_NosL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41247">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REPRESSOR YCNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41247:SF1">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REPRESSOR YCNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05573">
        <property type="entry name" value="NosL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160387">
        <property type="entry name" value="NosL/MerB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="199" mass="20901" checksum="6B5C5B233EC9A7EC" modified="2017-05-10" version="1">MSPDQSGSLSRRTVLVSAVAGGIGAVAGCLDGDRDGPDPVSLDDGQACDTCDMLIEMHPGPVGQAFYGDDAPRSLPDDRDDGAAWFCSSTCAYQFILEEEERGHEPAISYGTDYSTVDYTLRDDNGVTVISAHLEADAFADLHDLTFVADSDAEGAMGGSLIGFSDPDDAEAFQSEHGGELLAHDEVTREVLTGLGMGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXZ5</accession>
    <name>A0A1U7EXZ5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glyoxalase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3984A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323699.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXZ5"/>
    <dbReference type="STRING" id="348780.NP_3984A"/>
    <dbReference type="EnsemblBacteria" id="CAI50083">
        <property type="protein sequence ID" value="CAI50083"/>
        <property type="gene ID" value="NP_3984A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701928"/>
    <dbReference type="KEGG" id="nph:NP_3984A"/>
    <dbReference type="eggNOG" id="arCOG02709">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046006_2_2_2"/>
    <dbReference type="OrthoDB" id="6111at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004493">
        <property type="term" value="F:methylmalonyl-CoA epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046491">
        <property type="term" value="P:L-methylmalonyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051785">
        <property type="entry name" value="MMCE/EMCE_epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43048:SF5">
        <property type="entry name" value="BLR5325 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43048">
        <property type="entry name" value="METHYLMALONYL-COA EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="2">
        <location>
            <begin position="17"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="151" mass="15773" checksum="B0FB03B976665095" modified="2017-05-10" version="1">MTDADSTADATAAERPTAHHVGLTVSDLERAIAFYTDLFDCTVESRFTVDGEAFETVVGVDGAAGSFAHLDAGGTRLELVAYEPAGEPTPSAELVQPGAAHVAFEVADVDAFVNDLPDGVAPLSDPQTTASGTRLVFLRDPDGNRIELLEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXZ7</accession>
    <name>A0A1U7EXZ7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MoaD family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3946A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323681.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXZ7"/>
    <dbReference type="STRING" id="348780.NP_3946A"/>
    <dbReference type="EnsemblBacteria" id="CAI50064">
        <property type="protein sequence ID" value="CAI50064"/>
        <property type="gene ID" value="NP_3946A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702928"/>
    <dbReference type="KEGG" id="nph:NP_3946A"/>
    <dbReference type="eggNOG" id="arCOG00536">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114601_1_2_2"/>
    <dbReference type="OrthoDB" id="134663at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd17040">
        <property type="entry name" value="Ubl_MoaD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054834">
        <property type="entry name" value="SAMP1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003749">
        <property type="entry name" value="ThiS/MoaD-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041918">
        <property type="entry name" value="SAMP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02597">
        <property type="entry name" value="ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="MoaD/ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="97" mass="10128" checksum="492C915D9C6E6EBD" modified="2017-05-10" version="1">MQLTVEFHGTLRDAADGSSVTRQFGDDQTLGAVLEALADDYESLGPLVFGSDGRIRSNVAVAVNGDPVTGEPRRDRQLSDGDRLMLAPGLAGGGGNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY77</accession>
    <name>A0A1U7EY77_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4144A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50163.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323779.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY77"/>
    <dbReference type="STRING" id="348780.NP_4144A"/>
    <dbReference type="EnsemblBacteria" id="CAI50163">
        <property type="protein sequence ID" value="CAI50163"/>
        <property type="gene ID" value="NP_4144A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701984"/>
    <dbReference type="KEGG" id="nph:NP_4144A"/>
    <dbReference type="eggNOG" id="arCOG04550">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_156330_0_0_2"/>
    <dbReference type="OrthoDB" id="284200at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043903">
        <property type="entry name" value="DUF5785"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19098">
        <property type="entry name" value="DUF5785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50163.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="100" mass="11266" checksum="0EA490DDA394AEC8" modified="2017-05-10" version="1">MDWPHDPDGEEGSEGKRKYGQAILAKKLDEEGDFPLSASDFVDEHGDEPVRLNHSEVVSVADIFEHVDQSEFDDIVSFHKAVGQAMRNADMWEVDVEKHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY86</accession>
    <name>A0A1U7EY86_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Redoxin domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_4154A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323784.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY86"/>
    <dbReference type="STRING" id="348780.NP_4154A"/>
    <dbReference type="EnsemblBacteria" id="CAI50168">
        <property type="protein sequence ID" value="CAI50168"/>
        <property type="gene ID" value="NP_4154A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701987"/>
    <dbReference type="KEGG" id="nph:NP_4154A"/>
    <dbReference type="eggNOG" id="arCOG00314">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076204_1_0_2"/>
    <dbReference type="OrthoDB" id="334137at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016209">
        <property type="term" value="F:antioxidant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02969">
        <property type="entry name" value="PRX_like1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047262">
        <property type="entry name" value="PRX-like1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43640">
        <property type="entry name" value="OS07G0260300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43640:SF1">
        <property type="entry name" value="THIOREDOXIN-DEPENDENT PEROXIREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="10"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="189" mass="20576" checksum="5CFA7555CC7A4183" modified="2017-05-10" version="1">MALESASDELDVGDPAPAFELPGVDGDQHSLDDFGGYDALLVVFMCNHCPYVQAKFETMNRLADDYDDLAVVGINPNDADEYPEDSFETMVEYADRGDIDITAYLRDEPQSTAEAYGAVCTPDPFLFHNDGGAFRLVYHGRFDDALAPDDEPSEAPGFEMRAAIEAVLNGAEPEPAPGPSRGCSIKWLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY87</accession>
    <name>A0A1U7EY87_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">FAD-dependent oxidoreductase (Homolog to geranylgeranyl reductase)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4168A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323791.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY87"/>
    <dbReference type="STRING" id="348780.NP_4168A"/>
    <dbReference type="EnsemblBacteria" id="CAI50175">
        <property type="protein sequence ID" value="CAI50175"/>
        <property type="gene ID" value="NP_4168A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701992"/>
    <dbReference type="KEGG" id="nph:NP_4168A"/>
    <dbReference type="eggNOG" id="arCOG00570">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024648_2_0_2"/>
    <dbReference type="OrthoDB" id="192187at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011777">
        <property type="entry name" value="Geranylgeranyl_Rdtase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050407">
        <property type="entry name" value="Geranylgeranyl_reductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02032">
        <property type="entry name" value="GG-red-SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685:SF18">
        <property type="entry name" value="DIGERANYLGERANYLGLYCEROPHOSPHOLIPID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685">
        <property type="entry name" value="GERANYLGERANYL DIPHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="1">
        <location>
            <begin position="5"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="456" mass="49839" checksum="90CDF5485FBE7BC9" modified="2017-05-10" version="1">MTTFEYDVAIVGAGTAGCYAGATIADAGYDVVIVDRKDAEEAGHIACGDALKGADTFPEAIPKSEIESSFTNTDVDHGQFEIPQEDVSLDIPIPGELAVIDRWEYGRQLIDAAEDAGTEFHYDTVVQDVLQDDDGRVYGFSAIKKGDPRTYEAEIVLDGAGALSILQDKADFEDATFDTNVQYSQFSSAYREVIEVDEPVEWDDALVFKPTKRSAGYLWYFPRTPTEINVGLGFQMNEEPMKLVDDLREDISRRPELKGATVKDKLGAALPTRRPYDSATAPGYMAIGDAAAHVNPISGGGIAGAAYAGQYAGEAAIGAIEDGDVSEANLWEYNERVMDHYGGRYAALDVYNIFSTACDLDELLGLLAAMPAEKLSDALYSGSAEVGLPLKLRVLVKSFGHWKTLKELFDTKQLADRLLEHYETYPSSPEGLDAWQETRDEIMDDIYAQTGAEPKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYA4</accession>
    <name>A0A1U7EYA4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">O-acetyltransferase (Homolog to galactoside O-acetyltransferase)</fullName>
            <ecNumber evidence="3">2.3.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">yvoF</name>
        <name evidence="3" type="synonym">maa3</name>
        <name evidence="3" type="ordered locus">NP_4198A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323806.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYA4"/>
    <dbReference type="STRING" id="348780.NP_4198A"/>
    <dbReference type="EnsemblBacteria" id="CAI50190">
        <property type="protein sequence ID" value="CAI50190"/>
        <property type="gene ID" value="NP_4198A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702896"/>
    <dbReference type="KEGG" id="nph:NP_4198A"/>
    <dbReference type="eggNOG" id="arCOG01850">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051638_16_1_2"/>
    <dbReference type="OrthoDB" id="30669at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04647">
        <property type="entry name" value="LbH_MAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050179">
        <property type="entry name" value="Trans_hexapeptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43300">
        <property type="entry name" value="ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43300:SF11">
        <property type="entry name" value="ACETYLTRANSFERASE RV3034C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="178" mass="19466" checksum="1ACE2EC4EFB829B4" modified="2017-05-10" version="1">MTDAADSPADDDTAGERRHDRLRRHATPGPRNSLWSWPEAKHPLRVALNYVVIVLARHSPSLRLKNWLLRRIGVTVGTGVSWGLESTPDVFWPELVTVDDDAVIGYDTTLLCHEFLQDEYRTGQVRIGRRAMVGAGTIVLPGVEIGDGAQVAANSLVTEDVPPGETWAGVPAEPVNRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYB5</accession>
    <name>A0A1U7EYB5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Bifunctional protein GlmU</fullName>
            <ecNumber evidence="5">2.3.1.157</ecNumber>
            <ecNumber evidence="6">2.7.7.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">graD6</name>
        <name evidence="17" type="ordered locus">NP_4276A</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N-acetyl-alpha-D-glucosamine 1-phosphate + UTP + H(+) = UDP-N-acetyl-alpha-D-glucosamine + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13509"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="EC" id="2.7.7.23"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>alpha-D-glucosamine 1-phosphate + acetyl-CoA = N-acetyl-alpha-D-glucosamine 1-phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13725"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="ChEBI" id="CHEBI:58516"/>
            <dbReference type="EC" id="2.3.1.157"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route II): step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.157" evidence="5"/>
    <dbReference type="EC" id="2.7.7.23" evidence="6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323845.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYB5"/>
    <dbReference type="STRING" id="348780.NP_4276A"/>
    <dbReference type="EnsemblBacteria" id="CAI50229">
        <property type="protein sequence ID" value="CAI50229"/>
        <property type="gene ID" value="NP_4276A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702775"/>
    <dbReference type="KEGG" id="nph:NP_4276A"/>
    <dbReference type="eggNOG" id="arCOG00666">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_0_1_2"/>
    <dbReference type="OrthoDB" id="15372at2157"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00532"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019134">
        <property type="term" value="F:glucosamine-1-phosphate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003977">
        <property type="term" value="F:UDP-N-acetylglucosamine diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05636">
        <property type="entry name" value="LbH_G1P_TT_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04181">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023915">
        <property type="entry name" value="Bifunctiontional_GlmU_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050065">
        <property type="entry name" value="GlmU-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056729">
        <property type="entry name" value="GMPPB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03992">
        <property type="entry name" value="Arch_glmU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43584:SF8">
        <property type="entry name" value="N-ACETYLMURAMATE ALPHA-1-PHOSPHATE URIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43584">
        <property type="entry name" value="NUCLEOTIDYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25087">
        <property type="entry name" value="GMPPB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="9 17" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 17" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="15">
        <location>
            <begin position="3"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="Mannose-1-phosphate guanyltransferase C-terminal" evidence="16">
        <location>
            <begin position="260"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="379"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="382"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF25087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAI50229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="398" mass="41398" checksum="308058EA0EE39A03" modified="2017-05-10" version="1">MQTVILAAGKGTRMRPLTEAVPKPMLPVADRPLCAHAVDAAVEAGASELVFVVGYGADTVREYFGDQYRGVEVSYAVQTEQRGTADALSAAVELLSGEFAVLNGDSLYPAAGLRRLFESGPAIATATVEDPTAYGVVSTAAGDRVTEIVEKPDDPPTRLANAGAYVFPASARSWLDVDASERGEFELTDVVAHTIEETAVTAVQLSRWLDVGHPWELLAANEQELDTLDRRIAGTVADDATLEGSVVVEQGATVESGVVIEGPALVRKGASVGPNAYVRGATVVGEGCHIGHGVEVKNTVMLPEAAVPHLSYVGDSLIGHGANLGAGTQVANLRHDGATVKQTVAETRRSTGRRKYGAVVGPGAKTGVNTSIAPGVVLGADARTDPGESVCRDRRPDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYH1</accession>
    <name>A0A1U7EYH1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable oxidoreductase (Short-chain dehydrogenase family)</fullName>
            <ecNumber evidence="2">1.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4370A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323892.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYH1"/>
    <dbReference type="STRING" id="348780.NP_4370A"/>
    <dbReference type="EnsemblBacteria" id="CAI50276">
        <property type="protein sequence ID" value="CAI50276"/>
        <property type="gene ID" value="NP_4370A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702042"/>
    <dbReference type="KEGG" id="nph:NP_4370A"/>
    <dbReference type="eggNOG" id="arCOG01259">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_2_2"/>
    <dbReference type="OrthoDB" id="194879at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032787">
        <property type="term" value="P:monocarboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050259">
        <property type="entry name" value="SDR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005559">
        <property type="entry name" value="PRK07231.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009466">
        <property type="entry name" value="PRK12826.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42879">
        <property type="entry name" value="3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42879:SF2">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] REDUCTASE FABG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="263" mass="27707" checksum="DBD6B3FFACC1918A" modified="2017-05-10" version="1">MPTDQFSVDGQNVIVTGASQGIGRGIAERFAADGANVAICSRAQERIDPVAESIREDGGTALAVECNVREPEDVEAFVEATAEEFGGIDVLVNNAGGEFVAAFEDISENGWKSIIDLNLHGTFHCTQAAGEYMRDDGGGCIINMSSVNGQHAAPNESHYSASKAAIIRLTETLAVEWASDGIRVNCIAPGLVQTPGVAETLGIQEDQMPPRETVDRRIGHTEDIADVAQFLASDAAAFMTGETVTAKGVPRPGNSFEVDLGLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYH3</accession>
    <name>A0A1U7EYH3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable secreted glycoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4392A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323903.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYH3"/>
    <dbReference type="STRING" id="348780.NP_4392A"/>
    <dbReference type="EnsemblBacteria" id="CAI50287">
        <property type="protein sequence ID" value="CAI50287"/>
        <property type="gene ID" value="NP_4392A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702047"/>
    <dbReference type="KEGG" id="nph:NP_4392A"/>
    <dbReference type="HOGENOM" id="CLU_764234_0_0_2"/>
    <dbReference type="OrthoDB" id="386032at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="362" mass="40487" checksum="A341919BA7AA1D1F" modified="2017-05-10" version="1">MKRRYYLGTIAAAALAGCSELQPEEAPEETPEPEPSPEETPESEPAPDETPAPDDPEPTPEDEPPSDAEQFAESRIQSGDEQLREALSAYADAGNSESFLDVRASTTGFRWVPVYRHVQAANEQFARAANRGTAAQRTRVDTLRRVGALLTEMARTQERVATAFESFGDIVVAHQGDTVSPVAWRRLDERMATIDTRFDRLTDAGDPSDADASDHVSADELANKTDQLAAERDAFHRLLDARETYTQAHDDWIRGERLYRRRSWTSAEQRFDQAASAFEDASERLAADPVDDRRFDTRYQTFVRMAEALAEAATEYGASAAAYRDRDTETGDGRRRDGRRILRNEDVIDGLPSVRRLEAFEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYK3</accession>
    <name>A0A1U7EYK3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">HTH-10 family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_4436A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323924.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYK3"/>
    <dbReference type="STRING" id="348780.NP_4436A"/>
    <dbReference type="EnsemblBacteria" id="CAI50309">
        <property type="protein sequence ID" value="CAI50309"/>
        <property type="gene ID" value="NP_4436A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702062"/>
    <dbReference type="KEGG" id="nph:NP_4436A"/>
    <dbReference type="eggNOG" id="arCOG02274">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092678_0_0_2"/>
    <dbReference type="OrthoDB" id="194393at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007050">
        <property type="entry name" value="HTH_bacterioopsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056493">
        <property type="entry name" value="HVO_0513_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236:SF1">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04967">
        <property type="entry name" value="HTH_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24278">
        <property type="entry name" value="HVO_0513_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="Sigma3 and sigma4 domains of RNA polymerase sigma factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HVO-0513-like N-terminal" evidence="4">
        <location>
            <begin position="16"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="HTH bat-type" evidence="3">
        <location>
            <begin position="166"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI50309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="222" mass="24029" checksum="77FEA43DDF7872A6" modified="2017-05-10" version="1">MRYATVTITPESGAFQPADGAVADDPAVVRESIDQLESLDDGTCVMLSRVRGDLSRAADILDGQSDVLGYDIATDGDSRVGQQHGYIYVHFRPTETVAELLAAVEETEAVLKTPLEYRDNGGVRVTLIGDDETIQRTVEAVPNTVRISLEGTGDYRPSTGRLLSVLTPRQREILEAAVEAGYYEVPRQATHEDIAETVDVSAGTVGEHLRKVEGKLLSSLVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYM9</accession>
    <name>A0A1U7EYM9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">O-acetyltransferase (Homolog to galactoside O-acetyltransferase)</fullName>
            <ecNumber evidence="4">2.3.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">maa1</name>
        <name evidence="4" type="ordered locus">NP_4490A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323951.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYM9"/>
    <dbReference type="STRING" id="348780.NP_4490A"/>
    <dbReference type="EnsemblBacteria" id="CAI50336">
        <property type="protein sequence ID" value="CAI50336"/>
        <property type="gene ID" value="NP_4490A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702895"/>
    <dbReference type="KEGG" id="nph:NP_4490A"/>
    <dbReference type="eggNOG" id="arCOG01848">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065082_0_0_2"/>
    <dbReference type="OrthoDB" id="209943at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04647">
        <property type="entry name" value="LbH_MAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051159">
        <property type="entry name" value="Hexapeptide_acetyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23416:SF23">
        <property type="entry name" value="ACETYLTRANSFERASE C18B11.09C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23416">
        <property type="entry name" value="SIALIC ACID SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="260"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="265"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="290"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="301" mass="34156" checksum="F8767A82788F348E" modified="2017-05-10" version="1">MTKRHVSLPATAEEGLEAFLETVDRRLSSDEDTCQVVEDVLVDLHGDREAYERWQSGDDVSPAERVRLQGYDPCNATLESEYYAEKDEAAFEESKHLQWLWRQFDATPMADNVEFALRFRRMLADHLFEECGDGCRFFKGITFTYGHNISIGDNVVVHDDVHLDDRGELTIGDRVSISDGVHVYSHDHDIVDQTEVDNYHTIIEDDVRLTFDAMVRAGVQVGENAVVGARAVVQSDVPAHHVVVGQPAKSVRVKPGWESVAEPVDADHHDGRSDRKLPYELPDGLEQFDEFQRDLQPPDRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYR9</accession>
    <name>A0A1U7EYR9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Small CPxCG-related zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4582A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323997.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYR9"/>
    <dbReference type="EnsemblBacteria" id="CAI50382">
        <property type="protein sequence ID" value="CAI50382"/>
        <property type="gene ID" value="NP_4582A"/>
    </dbReference>
    <dbReference type="GeneID" id="54763327"/>
    <dbReference type="KEGG" id="nph:NP_4582A"/>
    <dbReference type="eggNOG" id="arCOG04657">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3003058_0_0_2"/>
    <dbReference type="OrthoDB" id="280204at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057160">
        <property type="entry name" value="DUF7838"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25208">
        <property type="entry name" value="DUF7838"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7838" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="53" mass="5888" checksum="BEE6F29940D59AC5" modified="2017-05-10" version="1">MSLSLERTCPECGTEEFYKAASMQIHLGTKKKWHCTECEYGFVAINGIDTASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYT9</accession>
    <name>A0A1U7EYT9_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Bifunctional protein GlmU</fullName>
            <ecNumber evidence="5">2.3.1.157</ecNumber>
            <ecNumber evidence="6">2.7.7.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">graD2</name>
        <name evidence="16" type="ordered locus">NP_4656A</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N-acetyl-alpha-D-glucosamine 1-phosphate + UTP + H(+) = UDP-N-acetyl-alpha-D-glucosamine + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13509"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="EC" id="2.7.7.23"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>alpha-D-glucosamine 1-phosphate + acetyl-CoA = N-acetyl-alpha-D-glucosamine 1-phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13725"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="ChEBI" id="CHEBI:58516"/>
            <dbReference type="EC" id="2.3.1.157"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route II): step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.157" evidence="5"/>
    <dbReference type="EC" id="2.7.7.23" evidence="6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324033.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYT9"/>
    <dbReference type="STRING" id="348780.NP_4656A"/>
    <dbReference type="EnsemblBacteria" id="CAI50419">
        <property type="protein sequence ID" value="CAI50419"/>
        <property type="gene ID" value="NP_4656A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702771"/>
    <dbReference type="KEGG" id="nph:NP_4656A"/>
    <dbReference type="eggNOG" id="arCOG00666">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_0_1_2"/>
    <dbReference type="OrthoDB" id="15372at2157"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00532"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019134">
        <property type="term" value="F:glucosamine-1-phosphate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003977">
        <property type="term" value="F:UDP-N-acetylglucosamine diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05636">
        <property type="entry name" value="LbH_G1P_TT_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04181">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023915">
        <property type="entry name" value="Bifunctiontional_GlmU_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050065">
        <property type="entry name" value="GlmU-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056729">
        <property type="entry name" value="GMPPB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03992">
        <property type="entry name" value="Arch_glmU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43584:SF8">
        <property type="entry name" value="N-ACETYLMURAMATE ALPHA-1-PHOSPHATE URIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43584">
        <property type="entry name" value="NUCLEOTIDYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25087">
        <property type="entry name" value="GMPPB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="9 16" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="14">
        <location>
            <begin position="3"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Mannose-1-phosphate guanyltransferase C-terminal" evidence="15">
        <location>
            <begin position="262"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF25087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAI50419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="397" mass="42081" checksum="678DB5B868B40A3C" modified="2017-05-10" version="1">MDAVVIAAGRGTRMRPLTETRPKPLLPVGEASLLERTLSQCVGLVDRFVIVVGYRDDMIRGRIGDNYEGVPVEYVKQTERLGTAHAVEQAADIVSDSFLVLNGDVVCDKPLLRQLADADGHAMATTTVSDPTSYGVVETEAGAVSALYEKPDDPPTDRINVGIYAFEPSVFEAIAATAESPRGEYELTDAIERLIDAGERVTTVDYDGAWIDVGRPWELLEATTATLDGLETRLAGTVEEGAHLHGPVVVQEGARVRSGAYIEGPVVIHKGADVGPNAYVRGATVIGPDVRVGNGVEIKNSVLMAHTAVGHLSYVGDSVLGADVNVGAGTMVANLRHDDESVRVTVKGDRVDTGRRKLGVIVGDRAKTGINTSLNAGVVLGVDARTAPGETVTRDRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYW7</accession>
    <name>A0A1U7EYW7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Bifunctional protein GlmU</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">graD4</name>
        <name evidence="4" type="ordered locus">NP_4680A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324045.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYW7"/>
    <dbReference type="STRING" id="348780.NP_4680A"/>
    <dbReference type="EnsemblBacteria" id="CAI50431">
        <property type="protein sequence ID" value="CAI50431"/>
        <property type="gene ID" value="NP_4680A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702773"/>
    <dbReference type="KEGG" id="nph:NP_4680A"/>
    <dbReference type="eggNOG" id="arCOG00666">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_4_0_2"/>
    <dbReference type="OrthoDB" id="15372at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04181">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42883">
        <property type="entry name" value="GLUCOSE-1-PHOSPHATE THYMIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42883:SF2">
        <property type="entry name" value="THYMIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="3">
        <location>
            <begin position="3"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="156"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="163"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="357" mass="37643" checksum="896ED558CB7841E2" modified="2017-05-10" version="1">MDAIVLAGGYATRLWPVTRHRPKMLLPLGERTVLDRLLAELEAESRIDDVFVSTNAQFETLIRNHLAETDFEKPQVSVESTTGEDEKLGVIAALAQLVEREALDDDTLVVGGDNVLGLSLARFCDQFEAHGGPTLAAYDVGTPAAASSYGVLETAAPAGQTDGGSDTAAATPTDSDAEVGGDTSLEVTTFAEKPDDPSSSLVSVACYGFPAELLSKFDAYLAAGNNPDEPGWFVKWLVEETPETVQAVPFDDAWFDVGTPESYLNAVAWALDGDSYVHPEAAIGDDVTLGSNVHVMADAEITDSSLERTVVFPEATIADSELERTLVDTGAELESVALSESVIGAYTRLGGAGGRPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYW9</accession>
    <name>A0A1U7EYW9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">FMN-binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4722A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324065.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYW9"/>
    <dbReference type="STRING" id="348780.NP_4722A"/>
    <dbReference type="EnsemblBacteria" id="CAI50452">
        <property type="protein sequence ID" value="CAI50452"/>
        <property type="gene ID" value="NP_4722A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702158"/>
    <dbReference type="KEGG" id="nph:NP_4722A"/>
    <dbReference type="eggNOG" id="arCOG00513">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02452">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_741030_0_0_2"/>
    <dbReference type="OrthoDB" id="953at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055927">
        <property type="entry name" value="DUF7504"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024747">
        <property type="entry name" value="Pyridox_Oxase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24336">
        <property type="entry name" value="DUF7504"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12900">
        <property type="entry name" value="Pyridox_ox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="388" mass="42408" checksum="CCCB2132765E4B2C" modified="2017-05-10" version="1">MTSTGHESEAAGPTLYLESSELDPTESFCTDVFENAADGEYRVVQVTATQSFESVRDALNTQLEQINDPSEAAVIITTPQAEKEATRGQVGEDTPLYGFRVSPDDLTGISIAFSQLIEKWEAEDAPVKICLRDIESLLPYHDAELVYRFLNTVLATLQGAGADVHAHFRSGATDDQSLQLFRSLFDTVVDHEASPLQSVAETEPDAEQAPSTASGEATLSGDDEDAADKETAPEPDSVSSGEMSNEEIDTFLSGEGYGTLAFGGDKPYAIPMSYGYDDSERVLYMHMGLFEGSEKASRIRDQTPASLVVTRYERPDKWRSVVVEGTVSKLSRDEVREREVVTAFAKSELASVDIFVPDLSEVDFEWYMLDPETLSGRRSVGSMAGAER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYY7</accession>
    <name>A0A1U7EYY7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">SirR/DtxR family transcription regulator SirR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">sirR2</name>
        <name evidence="8" type="ordered locus">NP_4726A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DtxR/MntR family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324067.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYY7"/>
    <dbReference type="STRING" id="348780.NP_4726A"/>
    <dbReference type="EnsemblBacteria" id="CAI50454">
        <property type="protein sequence ID" value="CAI50454"/>
        <property type="gene ID" value="NP_4726A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702160"/>
    <dbReference type="KEGG" id="nph:NP_4726A"/>
    <dbReference type="eggNOG" id="arCOG02099">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069532_0_2_2"/>
    <dbReference type="OrthoDB" id="24735at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.10">
        <property type="entry name" value="Iron dependent repressor, metal binding and dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050536">
        <property type="entry name" value="DtxR_MntR_Metal-Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007167">
        <property type="entry name" value="Fe-transptr_FeoA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001367">
        <property type="entry name" value="Fe_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036421">
        <property type="entry name" value="Fe_dep_repressor_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022687">
        <property type="entry name" value="HTH_DTXR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022689">
        <property type="entry name" value="Iron_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008988">
        <property type="entry name" value="Transcriptional_repressor_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33238">
        <property type="entry name" value="IRON (METAL) DEPENDENT REPRESSOR, DTXR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33238:SF7">
        <property type="entry name" value="IRON-DEPENDENT TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02742">
        <property type="entry name" value="Fe_dep_repr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01325">
        <property type="entry name" value="Fe_dep_repress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04023">
        <property type="entry name" value="FeoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00899">
        <property type="entry name" value="FeoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00529">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50037">
        <property type="entry name" value="C-terminal domain of transcriptional repressors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47979">
        <property type="entry name" value="Iron-dependent repressor protein, dimerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50944">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH dtxR-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI50454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="223" mass="24090" checksum="D3C34596081D4520" modified="2017-05-10" version="1">MDASPVVEEYVKQLHRLEAEHGRRIRTTELAEAVDRTKASTTSMVQKLDERGLVEHEEYGGTALTPTGEAVADRLVRKHRLLETFLVEQLDLPEAVAHDEADRLEHHVSDGVTDRLAELLGAPGGPAWDSRAVYPAAASAKRLTSGEPGESLVVEAVPHRQPKARSYLLERGVKPGAVIEVDEVGPAGLVTVTDAAGDRSVPVPGRLARYVGVRPPPGVDRND</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ08</accession>
    <name>A0A1U7EZ08_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NADPH:quinone reductase</fullName>
            <ecNumber evidence="3">1.6.5.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">qor1</name>
        <name evidence="3" type="ordered locus">NP_4816A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.6.5.5" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324111.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ08"/>
    <dbReference type="STRING" id="348780.NP_4816A"/>
    <dbReference type="EnsemblBacteria" id="CAI50499">
        <property type="protein sequence ID" value="CAI50499"/>
        <property type="gene ID" value="NP_4816A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703090"/>
    <dbReference type="KEGG" id="nph:NP_4816A"/>
    <dbReference type="eggNOG" id="arCOG01458">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_3_4_2"/>
    <dbReference type="OrthoDB" id="8709at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030554">
        <property type="term" value="F:adenyl nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043168">
        <property type="term" value="F:anion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003960">
        <property type="term" value="F:quinone reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08266">
        <property type="entry name" value="Zn_ADH_like1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051603">
        <property type="entry name" value="Zinc-ADH_QOR/CCCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44154">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44154:SF1">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="2">
        <location>
            <begin position="10"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="351" mass="37947" checksum="A21847DF19A81C5B" modified="2017-05-10" version="1">MKAIFYEEHGSTDVLQYDDFDDPEIGPEEVLVDIKAGGLNHLDVWTRKGMPSPEELPHIPGSDGAGVVSEVGERVTQFEAGDRVVVEPGLYCGECEFCRKGEESLCVDYKMLGEHVRGVHSELAAVPETNLIELPEHVDFVTAASAPLVFQTSWRMLMTRAEIQQSDSVLVLGASGGVGHACVQIAANEGCEVFATASSEEKLAHAEECGADHLINYEEEDFDEAIKELTDGRGVDVVVDHIGEATWDNSLSAAARGGTIVTCGATSGISPETNIPTVFWKQLDILGSTMGTPGEVDEVMTKVFDGTFEPHVREVLPMSEIARGHEMLESREGFGSVVVVPDSDYDPGEYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ20</accession>
    <name>A0A1U7EZ20_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Carbamoyl phosphate synthase small chain</fullName>
            <ecNumber evidence="10">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Carbamoyl phosphate synthetase glutamine chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10 12" type="primary">carA</name>
        <name evidence="12" type="ordered locus">NP_4830A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Small subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The small subunit (glutamine amidotransferase) binds and cleaves glutamine to supply the large subunit with the substrate ammonia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate + L-glutamate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the CarA family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.5" evidence="10"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324118.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
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            <begin position="2"/>
            <end position="122"/>
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    <sequence length="352" mass="37840" checksum="D2CF957B9234B4FE" modified="2017-05-10" version="1">MTDAYVALEDGRVVEARARAPGRTRGELVFTTAYTGYEESLTDPSYEEQILTFSYPLIGNYGVREERFESDRVHPRGVVARELTEDVAEWLEDEGVPAVDALDTRDLVTGIREQGAMKCGIAAGPDATPEDAEAELAECKGMSEHTDIGGQVSVDEQLTFNADGDGPTVALVDCGAKGSIIDSLVDRGATVEMLPYDATADDVKAVDPDVLFISNGPGDPKNFEAAADLVDEYAGNLPLAGICLGQQVIANALGGETEKMEFGHRGVNQPVMDLRTEKVVMTTQNHGYTVSEPGELDVTQVNVNDETPEGLESDELDIITRQYHPEANPGPHDTLGFFDDVLGMVEEPAATQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ77</accession>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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            <authorList>
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
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            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
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            <begin position="32"/>
            <end position="81"/>
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            <begin position="40"/>
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        </location>
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    </evidence>
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    <sequence length="410" mass="43811" checksum="E2A002DDF4C41CA6" modified="2017-05-10" version="1">MSQQRQTDGNDGRFTGRRAFIVGAAATGVAAFAGCTDNGNGDGNGTGNGTGNGTGNGDGNGGADSVAGASPLGQPVEPAGVSWDDLGDLEGEITVYSGRTPDQIDLVFEQLENEYSGLTINRDYDDNDAQVNQLMQEGEATPADVFYSQDPGALQAVADNGLTQVLPEDVIDTVPGSYRHPDGHWTGVSGRVRSVLYNSDRLDETPFDSWDELPDDIFSYAEDPRFEGIIATRPNSGTFRGFIQSMVELEGEETTRQWVSDFMDQDPQLFTSGSSHAEAINRGGDDDPIIGLGNSYYGARIVNENPDAPLRVAFTQDDPGALFSVAGVAATANVDDPELVAEFVRHLVAVEGQEFMMEDNGEFPVIDGVDYVGPLPGPDELNSPTFDLSEFDMELQGARELLEDEGMLPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ92</accession>
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        </submittedName>
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            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
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    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="entry name" value="Ribonucleotide Reductase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
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        <property type="entry name" value="RNR-like"/>
    </dbReference>
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        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="306" mass="34402" checksum="06239E2C2B4DFE2C" modified="2017-05-10" version="1">MDLDSTRQLPLDRESRGMRYYRNAVERHWDPHDIELDTDRENLKAFLDSTDTPETYLDGLRSGVARFGAGEQAVTEDLAPLATALGSIDDQMFVTTQIYEEAKHTDFFDRYWREVIHPVEDALGLDPSSPTDETWFNDAYEELFDRNEEAMERLLAAPTAEQFAKAYCHYHLVIEGILAQTGYYGMQQSYSADSYPELPHLPGLCDGFTKIRQDEGRHVGFGMAKLKALVGDGKVDPQLLDDIVGELLPLVDDITTDVGDESFDDVGIPPEELEAFAAEKHTERMEQIKDAAKSVPDLDSLTSLDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ97</accession>
    <name>A0A1U7EZ97_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">YqjG-type gamma-glutamylcysteinyl-hydroquinone reductase</fullName>
            <ecNumber evidence="6">1.8.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">NP_5048A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.8.-.-" evidence="6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324225.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ97"/>
    <dbReference type="STRING" id="348780.NP_5048A"/>
    <dbReference type="EnsemblBacteria" id="CAI50615">
        <property type="protein sequence ID" value="CAI50615"/>
        <property type="gene ID" value="NP_5048A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702238"/>
    <dbReference type="KEGG" id="nph:NP_5048A"/>
    <dbReference type="eggNOG" id="arCOG04712">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037263_1_0_2"/>
    <dbReference type="OrthoDB" id="172488at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03190">
        <property type="entry name" value="GST_C_Omega_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047047">
        <property type="entry name" value="GST_Omega-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016639">
        <property type="entry name" value="GST_Omega/GSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32419:SF6">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE OMEGA-LIKE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32419">
        <property type="entry name" value="GLUTATHIONYL-HYDROQUINONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13410">
        <property type="entry name" value="GST_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13409">
        <property type="entry name" value="GST_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015753">
        <property type="entry name" value="GST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01206">
        <property type="entry name" value="Xi.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01148">
        <property type="entry name" value="Xi_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="5">
        <location>
            <begin position="50"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="292"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="118"/>
            <end position="121"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="136"/>
            <end position="137"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Cys" evidence="3">
        <location>
            <position position="240"/>
        </location>
    </feature>
    <feature type="site" description="Lowers pKa of active site Cys" evidence="3">
        <location>
            <position position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR015753-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR015753-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR015753-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI50615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="319" mass="36691" checksum="1D832105D7026F1F" modified="2017-05-10" version="1">MHQFLDGEWVTEYEATNEDGEFERQTTTFRDRVDPEEATAGRYHLYVSYACPWAHRTLIARRLLGLEDVISVDVVDPYRAADGWQFSPEKDGCTPDTVNGFDYLREAYVAADPNYTGRVTVPVLWDTVEDTIVNNESREILRNLTTVFADLGNGVDLSPEGLRDDIDDALDRIYEPINNGVYRTGFADSQAAYDDAVAELFDALDYWNSVLEDQRYLVGDRLTEADIAMYTTLVRFDEVYHTHFMCNRRLIREYDTLWPYLRDLYTTLGFGETTRMDHIKEHYYTTHPDVSPKRIIPAGPAPDFEAPHDRDELAGEPPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ99</accession>
    <name>A0A1U7EZ99_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">VanZ family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5014A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324208.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ99"/>
    <dbReference type="STRING" id="348780.NP_5014A"/>
    <dbReference type="EnsemblBacteria" id="CAI50598">
        <property type="protein sequence ID" value="CAI50598"/>
        <property type="gene ID" value="NP_5014A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702228"/>
    <dbReference type="KEGG" id="nph:NP_5014A"/>
    <dbReference type="eggNOG" id="arCOG03232">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096028_4_1_2"/>
    <dbReference type="OrthoDB" id="214957at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037970">
        <property type="entry name" value="vanZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="122" mass="12614" checksum="7DF7C18C9553996E" modified="2017-05-10" version="1">MTRLRPRWAAAAGGWAAVLLVASVIDPGVADGGTTIPGGTAVFHVGGYAVLAVALSAAFRADDPRRLLVAVTVATLYGAAIELLQGQLPYRTMSALDVGYNAVGAAFGATLWWWRLPGGCCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZA8</accession>
    <name>A0A1U7EZA8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable demethylmenaquinone methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.163</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">menG</name>
        <name evidence="2" type="ordered locus">NP_5034A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.163" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324218.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZA8"/>
    <dbReference type="STRING" id="348780.NP_5034A"/>
    <dbReference type="EnsemblBacteria" id="CAI50608">
        <property type="protein sequence ID" value="CAI50608"/>
        <property type="gene ID" value="NP_5034A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702236"/>
    <dbReference type="KEGG" id="nph:NP_5034A"/>
    <dbReference type="eggNOG" id="arCOG04347">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051421_0_0_2"/>
    <dbReference type="OrthoDB" id="6027at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051741">
        <property type="term" value="F:2-methyl-6-phytyl-1,4-benzoquinone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043770">
        <property type="term" value="F:demethylmenaquinone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044649">
        <property type="entry name" value="MPBQ/MSBQ_MT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031164">
        <property type="entry name" value="SAM_MPBQ_MSBQ_MT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44516:SF11">
        <property type="entry name" value="2-METHYL-6-PHYTYL-1,4-HYDROQUINONE METHYLTRANSFERASE 2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44516">
        <property type="entry name" value="2-METHYL-6-PHYTYL-1,4-HYDROQUINONE METHYLTRANSFERASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51734">
        <property type="entry name" value="SAM_MPBQ_MSBQ_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MPBQ/MBSQ family SAM-binding methyltransferase profile" evidence="1">
        <location>
            <begin position="2"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="208" mass="23458" checksum="0655DAD59AC25EC8" modified="2017-05-10" version="1">MGVLEDKARARLFYKYLSKAYDTINPFIWNEEMRDEALTWFDIDDGDRVLDVGCGTGFATEGLLEHTDDVWGLDQSAHQLAKAYAKFGKRGTVNFHRGDAERLPFDDNSFDAYWSSGSIEYWPNPVDALAEARRVTKPGGTVLVVGPDYPNSTLFQKLADAIMLFYDEDEADRMFAAAGFETFEHRIQQRAPGTPRAITTIAEVPDEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZC9</accession>
    <name>A0A1U7EZC9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uracil-DNA glycosylase</fullName>
            <ecNumber evidence="9">3.2.2.27</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">udg2</name>
        <name evidence="9" type="ordered locus">NP_5088A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.2.2.27" evidence="9"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50635.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939886.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZC9"/>
    <dbReference type="STRING" id="348780.NP_5088A"/>
    <dbReference type="EnsemblBacteria" id="CAI50635">
        <property type="protein sequence ID" value="CAI50635"/>
        <property type="gene ID" value="NP_5088A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702250"/>
    <dbReference type="KEGG" id="nph:NP_5088A"/>
    <dbReference type="eggNOG" id="arCOG00905">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1159025_0_0_2"/>
    <dbReference type="OrthoDB" id="210606at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="entry name" value="Uracil-DNA glycosylase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051536">
        <property type="entry name" value="UDG_Type-4/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33693:SF1">
        <property type="entry name" value="TYPE-4 URACIL-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33693">
        <property type="entry name" value="TYPE-5 URACIL-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00986">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00987">
        <property type="entry name" value="UreE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uracil-DNA glycosylase-like" evidence="8">
        <location>
            <begin position="32"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI50635.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="215" mass="23565" checksum="EE0BA31F63B30175" modified="2017-05-10" version="1">MPTFPDRAARNALADDCQRCPELADCRTEISWGVGSSDATLVIVGEAPGAGNPDADRWRGGNYTGMSYTAQHSGRRIRELFESLEYAPDELYFTNAVKCFPSDGEGSNREPTSKERDNCRPYLLEELTAIEPACVVPTGRHATETLLAAADRTLDGFLDAVLTPIRTDGLPPLLPVLHPSYQAIWRSRLGYEADEYRHAIGRALSELGAGPRGQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZD4</accession>
    <name>A0A1U7EZD4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Phosphohexomutase (Phosphoglucomutase / phosphomannomutase)</fullName>
            <ecNumber evidence="11">5.4.2.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">pmm4</name>
        <name evidence="11" type="ordered locus">NP_5158A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.-" evidence="11"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324280.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZD4"/>
    <dbReference type="STRING" id="348780.NP_5158A"/>
    <dbReference type="EnsemblBacteria" id="CAI50670">
        <property type="protein sequence ID" value="CAI50670"/>
        <property type="gene ID" value="NP_5158A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703038"/>
    <dbReference type="KEGG" id="nph:NP_5158A"/>
    <dbReference type="eggNOG" id="arCOG00767">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016950_7_1_2"/>
    <dbReference type="OrthoDB" id="10363at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008966">
        <property type="term" value="F:phosphoglucosamine mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004615">
        <property type="term" value="F:phosphomannomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03087">
        <property type="entry name" value="PGM_like1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024086">
        <property type="entry name" value="GlmM_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050060">
        <property type="entry name" value="Phosphoglucosamine_mutase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03990">
        <property type="entry name" value="Arch_GlmM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42946:SF1">
        <property type="entry name" value="PHOSPHOGLUCOMUTASE (ALPHA-D-GLUCOSE-1,6-BISPHOSPHATE-DEPENDENT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42946">
        <property type="entry name" value="PHOSPHOHEXOSE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="Phosphoglucomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="8">
        <location>
            <begin position="3"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="9">
        <location>
            <begin position="150"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="10">
        <location>
            <begin position="259"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase C-terminal" evidence="7">
        <location>
            <begin position="392"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI50670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="458" mass="48281" checksum="34FCE7738768FE09" modified="2017-05-10" version="1">MDVFGSSGVRGVVGEKLTPPFVGRVAAAAATVLDADTMAVARDTRTSGQLFADAAASALAAVGCSVARLGVVPTPGAQAYADRTGRPVVMITASHNPPAYNGVKLVGGDGTELPRATLETIEDRLLAEDFDLVGYDAVGSSTRIPDAPETYVEELLDRLATDGLRERLAAAEPTVAIDPGNGAGCVTSPRFFRELGCDVVTVNGQPDGHFPGRDPEPVADNLGSLRRLVRSTDADLGIAHDGDADRAMFVDETGSHIEGDAALAALADAELEPGDAVVSAVNVSQRLVDVVADADAELELTQIGSTYLISRIRKLRREGTSVPVAGEGNGGIMFPDYRIARDGAYTAARFLALVADRPASDVVADYSGYAYLRANIEYDHPDEREAMLVAAEKVAEDADAELTTTDGYRLDYGDAWVLVRPSGTEPLVRLYAEAGDRARAQTLLETFRDPMEDAQAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZN1</accession>
    <name>A0A1U7EZN1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Pilin PilA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pilA2</name>
        <name evidence="4" type="ordered locus">NP_5358A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324378.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZN1"/>
    <dbReference type="STRING" id="348780.NP_5358A"/>
    <dbReference type="EnsemblBacteria" id="CAI50770">
        <property type="protein sequence ID" value="CAI50770"/>
        <property type="gene ID" value="NP_5358A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702349"/>
    <dbReference type="KEGG" id="nph:NP_5358A"/>
    <dbReference type="eggNOG" id="arCOG02416">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116126_0_0_2"/>
    <dbReference type="OrthoDB" id="118020at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013373">
        <property type="entry name" value="Flagellin/pilin_N_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012859">
        <property type="entry name" value="Pilin_N_archaeal"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02537">
        <property type="entry name" value="arch_flag_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38138:SF1">
        <property type="entry name" value="ARCHAEAL TYPE IV PILIN N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38138">
        <property type="entry name" value="VNG6441H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07790">
        <property type="entry name" value="Pilin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="Archaeal Type IV pilin N-terminal" evidence="3">
        <location>
            <begin position="15"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="178" mass="19053" checksum="1FE76E72DD7307D3" modified="2017-05-10" version="1">MNLSDLLNTETDDRRAVSPVIGVILMVAITVILAAVIGTFVLGLGDQVQETAPNAQISGEQITLDEGDLDDEEAVRITHESGDAINPNEIEITVDGQTAASVEPDSDVDEADTSDDGPTIWTGGDRISAGDRVQIVGTYDVDGDQFDEELDDEDEIRVTWQAPGGDRTATLFTFEVDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILV9</accession>
    <name>Q3ILV9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">UspA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_6262A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324508.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILV9"/>
    <dbReference type="EnsemblBacteria" id="CAI50911">
        <property type="protein sequence ID" value="CAI50911"/>
        <property type="gene ID" value="NP_6262A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694659"/>
    <dbReference type="KEGG" id="nph:NP_6262A"/>
    <dbReference type="HOGENOM" id="CLU_101252_0_0_2"/>
    <dbReference type="OrthoDB" id="157328at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="244" mass="26361" checksum="0015B62627EA09A0" modified="2005-11-08" version="1">MPLSTDSTILVPVDVSEPATPSQGVVDLLESVNVVLLGYFPVPDQTVPAQLKETRTEQAESRLVEAASRFSGGNGTLTTKLVFTHDRQDPIDRIAAEKQCDAVMIPGDGNGINRILVPLRGDRNLDRILGVVVTLLQNSDSSVTLSHTVGEEGDRGRGESVLEKAATQLLDEGIEPDRIERRIVECDDVPADIVGASDQFDLLVLGETEPSLRERIFGARLRTISQEVASPTLVVRNPNGTSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILY9</accession>
    <name>Q3ILY9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lrp/AsnC family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_6200A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324478.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILY9"/>
    <dbReference type="EnsemblBacteria" id="CAI50880">
        <property type="protein sequence ID" value="CAI50880"/>
        <property type="gene ID" value="NP_6200A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694639"/>
    <dbReference type="KEGG" id="nph:NP_6200A"/>
    <dbReference type="HOGENOM" id="CLU_091233_5_4_2"/>
    <dbReference type="OrthoDB" id="183514at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043200">
        <property type="term" value="P:response to amino acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154">
        <property type="entry name" value="LEUCINE-RESPONSIVE REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154:SF34">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR AZLB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator AsnC/Lrp ligand binding" evidence="1">
        <location>
            <begin position="81"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="165" mass="18802" checksum="2BB0452A8A1FBE17" modified="2005-11-08" version="1">MTGELDERDFRILCAIAEHETFSSEDLHEETGIPKSTVHYRIQNMKDEGVIKNELFEFDREKIGIEITLISEVWAEFGEGYHDTVGEKLSEIEGVNQVYFTMGDTDFVAIARLTSRDMVEQLVEDYESIDEIQRTSSKFAISTIKEDISISALGDYSVETLLDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM23</accession>
    <name>Q3IM23_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC-type transport system periplasmic substrate-binding protein (Probable substrate phosphate/phosphonate)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">phnD2</name>
        <name evidence="3" type="synonym">abc18s</name>
        <name evidence="3" type="ordered locus">NP_6126A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50843.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939910.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM23"/>
    <dbReference type="EnsemblBacteria" id="CAI50843">
        <property type="protein sequence ID" value="CAI50843"/>
        <property type="gene ID" value="NP_6126A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694678"/>
    <dbReference type="KEGG" id="nph:NP_6126A"/>
    <dbReference type="HOGENOM" id="CLU_051472_1_0_2"/>
    <dbReference type="OrthoDB" id="252027at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005770">
        <property type="entry name" value="PhnD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01098">
        <property type="entry name" value="3A0109s03R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35841">
        <property type="entry name" value="PHOSPHONATES-BINDING PERIPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35841:SF1">
        <property type="entry name" value="PHOSPHONATES-BINDING PERIPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12974">
        <property type="entry name" value="Phosphonate-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="72"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="76"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50843.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="368" mass="41055" checksum="186EBE346129E95F" modified="2012-10-03" version="2">MDDQCTRTRREMLAVGGAAGIGALAGCLGGENEEVDVNYDEFPDFDPLDPEYPQPAGRLIEEGFARASIEELEENFEPRDEPRYGRAPRDPETADEIIDPDPLVFAWTPGEEQQVYQEGLDPLIENISAETGRDVEVQVIDSYAAMTEAFRSERLHIARVAAGNVPFAVNMTGAVPFSMPYGDGTFGNRLWIITRIDNDDINTVDDLVGRDVVHTEQTSGSGHLEPSLFLEEQEGIVPGDDYDISFSGGHFRSVRGIYLGDYEVGPVAGPIPEVMDNDDEINAEELKVVWAGPFARPLGPMCYQYNLPEEVREGIKRAHFDYDYGGTDLYEIVNRFDTFAPIDYATHYDPVLQVHEEQGIEYIEDNLD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-03-24" modified="2026-01-28" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMD8</accession>
    <name>LYSY_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative [LysW]-L-2-aminoadipate/[LysW]-L-glutamate phosphate reductase</fullName>
            <ecNumber evidence="1">1.2.1.103</ecNumber>
            <ecNumber evidence="1">1.2.1.106</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lysY</name>
        <name type="ordered locus">NP_5260A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in both the arginine and lysine biosynthetic pathways.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[amino-group carrier protein]-C-terminal-N-(1-carboxy-5-oxopentan-1-yl)-L-glutamine + phosphate + NADP(+) = [amino-group carrier protein]-C-terminal-N-(1-carboxy-5-phosphooxy-5-oxopentan-1-yl)-L-glutamine + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9712"/>
            <dbReference type="Rhea" id="RHEA-COMP:9714"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78499"/>
            <dbReference type="ChEBI" id="CHEBI:78501"/>
            <dbReference type="EC" id="1.2.1.103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[amino-group carrier protein]-C-terminal-gamma-(L-glutamyl-5-semialdehyde)-L-glutamate + phosphate + NADP(+) = [amino-group carrier protein]-C-terminal-gamma-(5-phospho-L-glutamyl)-L-glutamate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52668"/>
            <dbReference type="Rhea" id="RHEA-COMP:13313"/>
            <dbReference type="Rhea" id="RHEA-COMP:13327"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:136717"/>
            <dbReference type="ChEBI" id="CHEBI:136761"/>
            <dbReference type="EC" id="1.2.1.106"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (Thermus route): step 3/5.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAGSA dehydrogenase family. Type 1 subfamily. LysY sub-subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.103" evidence="1"/>
    <dbReference type="EC" id="1.2.1.106" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324330.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMD8"/>
    <dbReference type="SMR" id="Q3IMD8"/>
    <dbReference type="STRING" id="348780.NP_5260A"/>
    <dbReference type="EnsemblBacteria" id="CAI50721">
        <property type="protein sequence ID" value="CAI50721"/>
        <property type="gene ID" value="NP_5260A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702517"/>
    <dbReference type="KEGG" id="nph:NP_5260A"/>
    <dbReference type="eggNOG" id="arCOG00495">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006384_0_1_2"/>
    <dbReference type="OrthoDB" id="372053at2157"/>
    <dbReference type="UniPathway" id="UPA00033">
        <property type="reaction ID" value="UER00037"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00068"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043870">
        <property type="term" value="F:N-acetyl-gamma-aminoadipyl-phosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003942">
        <property type="term" value="F:N-acetyl-gamma-glutamyl-phosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070401">
        <property type="term" value="F:NADP+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23939">
        <property type="entry name" value="AGPR_1_C_LysY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd24151">
        <property type="entry name" value="AGPR_1_N_LysY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00150">
        <property type="entry name" value="ArgC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02083">
        <property type="entry name" value="LysY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050085">
        <property type="entry name" value="AGPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058924">
        <property type="entry name" value="AGPR_dimerisation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000706">
        <property type="entry name" value="AGPR_type-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037535">
        <property type="entry name" value="LysY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01850">
        <property type="entry name" value="argC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32338:SF11">
        <property type="entry name" value="[LYSW]-L-2-AMINOADIPATE_[LYSW]-L-GLUTAMATE PHOSPHATE REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32338">
        <property type="entry name" value="N-ACETYL-GAMMA-GLUTAMYL-PHOSPHATE REDUCTASE, CHLOROPLASTIC-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22698">
        <property type="entry name" value="Semialdhyde_dhC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0457">Lysine biosynthesis</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000096731" description="Putative [LysW]-L-2-aminoadipate/[LysW]-L-glutamate phosphate reductase">
        <location>
            <begin position="1"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="12"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02083"/>
        </source>
    </evidence>
    <sequence length="346" mass="36559" checksum="8B80450FAF67B3E0" modified="2005-11-08" version="1">MSGYTASVVGASGFTGGEVLRLLSGHPEMAVTQATSRSYENKTVGSIHPNLRGMDLRFSSPEELESVDVLFACTPHGVSMEHIDAFQDAADTVVDLSADFRLDIESQYDEWYDGHNRPELLDKAEYALPELNRENLPGADIVASGGCNATAAIMGLKPLFDGDILGGGEQIVVDVKVGSSEGGAGGGEASSHPERSGVVRPYAPTGHRHEAEIEQFLGTGVSFTAHAVDMTRGASATCHVYPEEPVSKGDLWSAFRESYEDEPFVRLVAGGSGVYRYPEPKAVAGSNYAEVGFELDPTNKRVVVFSAIDNMMKGSAGQAVHAANIALGIEETAGLEFAGLHPVGAP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-17" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMR2</accession>
    <name>PURQ_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribosylformylglycinamidine synthase subunit PurQ</fullName>
            <shortName evidence="1">FGAM synthase</shortName>
            <ecNumber evidence="1">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Formylglycinamide ribonucleotide amidotransferase subunit I</fullName>
            <shortName evidence="1">FGAR amidotransferase I</shortName>
            <shortName evidence="1">FGAR-AT I</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glutaminase PurQ</fullName>
            <ecNumber evidence="1">3.5.1.2</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphoribosylformylglycinamidine synthase subunit I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purQ</name>
        <name type="ordered locus">NP_5010A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + L-glutamine + ATP + H2O = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="1"/>
    <dbReference type="EC" id="3.5.1.2" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324206.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMR2"/>
    <dbReference type="SMR" id="Q3IMR2"/>
    <dbReference type="STRING" id="348780.NP_5010A"/>
    <dbReference type="EnsemblBacteria" id="CAI50596">
        <property type="protein sequence ID" value="CAI50596"/>
        <property type="gene ID" value="NP_5010A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703077"/>
    <dbReference type="KEGG" id="nph:NP_5010A"/>
    <dbReference type="eggNOG" id="arCOG00102">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001031_3_1_2"/>
    <dbReference type="OrthoDB" id="6486at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01740">
        <property type="entry name" value="GATase1_FGAR_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00421">
        <property type="entry name" value="PurQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010075">
        <property type="entry name" value="PRibForGlyAmidine_synth_PurQ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01737">
        <property type="entry name" value="FGAM_synth_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002957">
        <property type="entry name" value="PRK03619.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47552">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47552:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13507">
        <property type="entry name" value="GATase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001586">
        <property type="entry name" value="FGAM_synth_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01211">
        <property type="entry name" value="GATase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000252746" description="Phosphoribosylformylglycinamidine synthase subunit PurQ">
        <location>
            <begin position="1"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-1" evidence="1">
        <location>
            <begin position="2"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="202"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="224" mass="24031" checksum="3E4F0D50DFECBA86" modified="2005-11-08" version="1">MTVAIIRFGGSNCDRDAERALAHLDIDAEIVWHEDGLPEETTGVMIPGGFSYGDYLRAGAMAARAPIMDDVREQAEAGVPVLGVCNGAQIGSEGDLTPGAFTTNRSARFQCEPVYLRVENAETPWTEAYEDGEVIEVPIAHGEGRFEIADDELETLVDEDRVIFRYCDADGNVTDAANPNGSKDNVAGVLGEHESVAVLMPHPERASLPDIGPTDGQGVLEGFR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-03" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMW9</accession>
    <name>RL18_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL18</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L18</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl18</name>
        <name type="ordered locus">NP_4892A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This is one of the proteins that bind and probably mediate the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Contacts the 5S and 23S rRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324149.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMW9"/>
    <dbReference type="SMR" id="Q3IMW9"/>
    <dbReference type="STRING" id="348780.NP_4892A"/>
    <dbReference type="EnsemblBacteria" id="CAI50537">
        <property type="protein sequence ID" value="CAI50537"/>
        <property type="gene ID" value="NP_4892A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703127"/>
    <dbReference type="KEGG" id="nph:NP_4892A"/>
    <dbReference type="eggNOG" id="arCOG04088">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056222_2_0_2"/>
    <dbReference type="OrthoDB" id="8644at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00432">
        <property type="entry name" value="Ribosomal_L18_L5e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.100:FF:000008">
        <property type="entry name" value="50S ribosomal protein L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01337_A">
        <property type="entry name" value="Ribosomal_uL18_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057267">
        <property type="entry name" value="Rbsml_uL18_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005485">
        <property type="entry name" value="Rbsml_uL18_euk_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057268">
        <property type="entry name" value="Ribosomal_L18"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006342">
        <property type="entry name" value="PRK08569.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23410:SF12">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23410">
        <property type="entry name" value="RIBOSOMAL PROTEIN L5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17144">
        <property type="entry name" value="Ribosomal_L5e"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000251400" description="Large ribosomal subunit protein uL18">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="184" mass="20201" checksum="C8A3E62F54869851" modified="2005-11-08" version="1">MATGPRYTVPMRRRREARTNYHQRLRLLKSGKPRLVARKSNNQTKAQLVVTGPQGDETVASATSADLEAFGWEAPTGNLPAAYLTGLLAGKRAIEAGLDEAVLDIGLNTATPGNKVFAVQEGVIDAGLEVPHNEDVFADWQRTRGAHIAEYAEQLEDGLYSGDFDATELPDHFDEVRERVEDEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q3INI6</accession>
    <name>RL1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl1</name>
        <name type="ordered locus">NP_4452A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds directly to 23S rRNA. Probably involved in E site tRNA release.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Protein L1 is also a translational repressor protein, it controls the translation of its operon by binding to its mRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323932.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3INI6"/>
    <dbReference type="SMR" id="Q3INI6"/>
    <dbReference type="STRING" id="348780.NP_4452A"/>
    <dbReference type="EnsemblBacteria" id="CAI50317">
        <property type="protein sequence ID" value="CAI50317"/>
        <property type="gene ID" value="NP_4452A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703118"/>
    <dbReference type="KEGG" id="nph:NP_4452A"/>
    <dbReference type="eggNOG" id="arCOG04289">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062853_4_0_2"/>
    <dbReference type="OrthoDB" id="10382at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00403">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.790:FF:000005">
        <property type="entry name" value="50S ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01318_A">
        <property type="entry name" value="Ribosomal_uL1_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002143">
        <property type="entry name" value="Ribosomal_uL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_uL1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_uL1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023669">
        <property type="entry name" value="Ribosomal_uL1_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023673">
        <property type="entry name" value="Ribosomal_uL1_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003244">
        <property type="entry name" value="PRK04203.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36427">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36427:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL1M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002155">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="Ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01199">
        <property type="entry name" value="RIBOSOMAL_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0678">Repressor</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0810">Translation regulation</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000230654" description="Large ribosomal subunit protein uL1">
        <location>
            <begin position="1"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="212" mass="23250" checksum="F85CB44F5ACA0265" modified="2005-11-08" version="1">MADSIEDAVSRAMSESPERNFRETVDLAINLRDLDLADPNNRVDESIVLPSGTGQDTHIVVFAEGETALRAEDVADDVLDSDDLEDLGDDDNAAKDLADETDFFIAEADLMQDIGRYLGTVLGPRGKMPTPLQPDDDVVETVNRMKNTVQVRSGERRTFHTRVGAEDMDADAIADNVDVILRRLFTDLEKGPQNIDTVYVKTTMGPAVEVPA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-04-08" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IP08</accession>
    <name>TFE_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription factor E</fullName>
            <shortName evidence="1">TFE</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">TFIIE subunit alpha homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Transcription initiation factor TFIIE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tfe</name>
        <name type="ordered locus">NP_4106A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transcription factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Facilitates transcription initiation by enhancing TATA-box recognition by TATA-box-binding protein (Tbp), and transcription factor B (Tfb) and RNA polymerase recruitment. Not absolutely required for transcription in vitro, but particularly important in cases where Tbp or Tfb function is not optimal. It dynamically alters the nucleic acid-binding properties of RNA polymerases by stabilizing the initiation complex and destabilizing elongation complexes. Seems to translocate with the RNA polymerase following initiation and acts by binding to the non template strand of the transcription bubble in elongation complexes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer. Interaction with RNA polymerase subunits RpoF and RpoE is necessary for Tfe stimulatory transcription activity. Able to interact with Tbp and RNA polymerase in the absence of DNA promoter. Interacts both with the preinitiation and elongation complexes.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">The winged helix domain is involved in binding to DNA in the preinitiation complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TFE family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323760.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IP08"/>
    <dbReference type="SMR" id="Q3IP08"/>
    <dbReference type="STRING" id="348780.NP_4106A"/>
    <dbReference type="EnsemblBacteria" id="CAI50144">
        <property type="protein sequence ID" value="CAI50144"/>
        <property type="gene ID" value="NP_4106A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701974"/>
    <dbReference type="KEGG" id="nph:NP_4106A"/>
    <dbReference type="eggNOG" id="arCOG04270">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100097_0_0_2"/>
    <dbReference type="OrthoDB" id="5935at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000264">
        <property type="entry name" value="Transcription factor E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01909">
        <property type="entry name" value="TFE_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016481">
        <property type="entry name" value="TF_E_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039997">
        <property type="entry name" value="TFE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017919">
        <property type="entry name" value="TFIIE/TFIIEa_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002853">
        <property type="entry name" value="TFIIE_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024550">
        <property type="entry name" value="TFIIEa/SarR/Rpc3_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00373">
        <property type="entry name" value="transcription factor E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13097:SF7">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13097">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIE, ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02002">
        <property type="entry name" value="TFIIE_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006373">
        <property type="entry name" value="TF_E_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00531">
        <property type="entry name" value="TFIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51344">
        <property type="entry name" value="HTH_TFE_IIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000326614" description="Transcription factor E">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="HTH TFE/IIEalpha-type" evidence="1">
        <location>
            <begin position="8"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01909"/>
        </source>
    </evidence>
    <sequence length="175" mass="20503" checksum="EEEA75912C04572B" modified="2005-11-08" version="1">MAFEDLLNDPVIQKYLHELVGPTGMPVAAAPPDGEVTDEELAEELGLELNDVRRALFILYENDLASYRRLRDEDSGWLTYLWTFQYENIPEQLEEEMHRLLEALENRRQYESENEFYLCGECQLRFEFDEAMEFGFECPECGGRLESMENDRLVNAMEQHIEALRDELHVETAEA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-10-03" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IPL1</accession>
    <name>GFPS_NATPD</name>
    <protein>
        <recommendedName>
            <fullName>Geranylfarnesyl diphosphate synthase</fullName>
            <shortName>GFPS</shortName>
            <ecNumber>2.5.1.81</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Farnesylgeranyl diphosphate synthase</fullName>
            <shortName>FGPP synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">idsA3</name>
        <name type="ordered locus">NP_3696A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1994" name="FEBS Lett." volume="341" first="291" last="294">
            <title>A novel prenyltransferase, farnesylgeranyl diphosphate synthase, from the haloalkaliphilic archaeon, Natronobacterium pharaonis.</title>
            <authorList>
                <person name="Tachibana A."/>
            </authorList>
            <dbReference type="PubMed" id="8137956"/>
            <dbReference type="DOI" id="10.1016/0014-5793(94)80475-3"/>
        </citation>
        <scope>FUNCTION AS A GERANYLFARNESYL DIPHOSPHATE SYNTHASE</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Probably involved in biosynthesis of the precursor for C25 (sesterterpanyl chain) moiety of C20-C25 diether (2-O-sesterterpanyl-3-O-phytanyl-sn-glycer) membrane lipid. Catalyzes the condensation of isopentenyl pyrophosphate with the allylic pyrophosphates to yield geranylfarnesyl diphosphate (GFPP). Geranylgeranyl diphosphate (GGPP) is the preferred substrate, but dimethylallyl diphosphate (DMAPP) and farnesyl diphosphate (FPP) can also be used as allylic substrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>isopentenyl diphosphate + (2E,6E,10E)-geranylgeranyl diphosphate = (2E,6E,10E,14E)-geranylfarnesyl diphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:25694"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57907"/>
            <dbReference type="ChEBI" id="CHEBI:58756"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="2.5.1.81"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="4">Binds 2 Mg(2+) ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.81"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323557.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IPL1"/>
    <dbReference type="SMR" id="Q3IPL1"/>
    <dbReference type="STRING" id="348780.NP_3696A"/>
    <dbReference type="EnsemblBacteria" id="CAI49939">
        <property type="protein sequence ID" value="CAI49939"/>
        <property type="gene ID" value="NP_3696A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702862"/>
    <dbReference type="KEGG" id="nph:NP_3696A"/>
    <dbReference type="eggNOG" id="arCOG01726">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014015_2_1_2"/>
    <dbReference type="OrthoDB" id="26738at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044687">
        <property type="term" value="F:geranylfarnesyl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000002">
        <property type="entry name" value="Octaprenyl diphosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040789">
        <property type="entry name" value="gerfarn_diphsyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001:SF85">
        <property type="entry name" value="SHORT CHAIN ISOPRENYL DIPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01017">
        <property type="entry name" value="Polyprenyl_Transferase_Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000419207" description="Geranylfarnesyl diphosphate synthase">
        <location>
            <begin position="1"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>isopentenyl diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>isopentenyl diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>isopentenyl diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>an all-trans-polyprenyl diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58914"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>isopentenyl diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>an all-trans-polyprenyl diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58914"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>an all-trans-polyprenyl diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58914"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>an all-trans-polyprenyl diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58914"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P14324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="8137956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="341" mass="37411" checksum="F29F336AB8F96852" modified="2005-11-08" version="1">MTSADHVESAIAERREIVNEAVSEQLPVQKPERLYSASRYLLDAGGKRLRPTILLLAAESLADVEPLSADYRQFPSLPGDEVDVLSAAVSIEVIQSFTLIHDDIMDDDDLRRGVPAVHREYDLETAILAGDTLYSKAFEYMLDTGAPAERSVEALDELATTCTEICEGQALDVDFENRSDVTTEEYLEMVEFKTAVLYAAAASIPAILLGSDDETVEALHGYGLDIGRAFQIQDDLLDLTAPSDELGKQRGSDLVENKRTVITLHARDQGIDVEGLVSDDPSDAEIEAAVQTLEDAGSIDFAREMALDLVTSGKERLDVLPENEARQLLEDIADFLVERSY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-16" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IPX9</accession>
    <name>CRYD_NATPD</name>
    <protein>
        <recommendedName>
            <fullName>Cryptochrome DASH</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">cry</name>
        <name type="synonym">phr2</name>
        <name type="ordered locus">NP_3456A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May have a photoreceptor function. Binds DNA; probably functions as a transcriptional repressor.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="1">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
        </cofactor>
        <text evidence="1">Binds 1 5,10-methenyltetrahydrofolate (MTHF) per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNA photolyase class-1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323439.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IPX9"/>
    <dbReference type="SMR" id="Q3IPX9"/>
    <dbReference type="STRING" id="348780.NP_3456A"/>
    <dbReference type="EnsemblBacteria" id="CAI49819">
        <property type="protein sequence ID" value="CAI49819"/>
        <property type="gene ID" value="NP_3456A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703032"/>
    <dbReference type="KEGG" id="nph:NP_3456A"/>
    <dbReference type="eggNOG" id="arCOG02840">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010348_6_2_2"/>
    <dbReference type="OrthoDB" id="11721at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003904">
        <property type="term" value="F:deoxyribodipyrimidine photo-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000719">
        <property type="term" value="P:photoreactive repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.579.10">
        <property type="entry name" value="DNA Cyclobutane Dipyrimidine Photolyase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014133">
        <property type="entry name" value="Cry_DASH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036134">
        <property type="entry name" value="Crypto/Photolyase_FAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036155">
        <property type="entry name" value="Crypto/Photolyase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005101">
        <property type="entry name" value="Cryptochr/Photolyase_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002081">
        <property type="entry name" value="Cryptochrome/DNA_photolyase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006050">
        <property type="entry name" value="DNA_photolyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02765">
        <property type="entry name" value="crypto_DASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11455">
        <property type="entry name" value="CRYPTOCHROME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11455:SF22">
        <property type="entry name" value="CRYPTOCHROME DASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00875">
        <property type="entry name" value="DNA_photolyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03441">
        <property type="entry name" value="FAD_binding_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00147">
        <property type="entry name" value="DNAPHOTLYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48173">
        <property type="entry name" value="Cryptochrome/photolyase FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52425">
        <property type="entry name" value="Cryptochrome/photolyase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51645">
        <property type="entry name" value="PHR_CRY_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0157">Chromophore</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0678">Repressor</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000235315" description="Cryptochrome DASH">
        <location>
            <begin position="1"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="Photolyase/cryptochrome alpha/beta">
        <location>
            <begin position="2"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="161"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="161"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="474" mass="53666" checksum="9CEB7D792AED04F5" modified="2005-11-08" version="1">MDTAVVWFRDDLRVTDNPTLADAVAAAETVIPVYTFDPDRYTESEYGPPKTGGHRAVFRRQAVADLRASLRDRGGDLLVRSGRPATVVPELAQRAGADAVYAQTKPATEERRRAADVASALDDAGIALRQRWTHTLYHPDDLPTPPTDIDDTFTPWRKETEAAATVRDPRSAPETVPTPDGLTPGPVPTVESLGVSEPPTDDRAVLEFEGGESAGKRRVESYIWEGDHLREYKTTRNGLLGADYSSKFSPWLAAGCLSPRWLHREVERYEDERVANEDTYWLVFELAWRDFFQFQFEKYGATFFKPGGIRERNIDWDRDRALFERWADGETGVPFVDANMRELNRTGYMSNRGRQNVASFLAGVLGIDWRWGAAYFEARLIDYDVASNWGNWAYQAGVGNDSRDRYFDVRSQAERYDSDAEYITTWLPELAALPATIAHQPWQLSEAEQREYGVELGVDYPEPVVDIDARYQSL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IQF5</accession>
    <name>METK_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">S-adenosylmethionine synthase</fullName>
            <shortName evidence="1">AdoMet synthase</shortName>
            <ecNumber evidence="1">2.5.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Methionine adenosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mat</name>
        <name type="ordered locus">NP_3100A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of S-adenosylmethionine from methionine and ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-methionine + ATP + H2O = S-adenosyl-L-methionine + phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.5.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AdoMet synthase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.6" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323263.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IQF5"/>
    <dbReference type="SMR" id="Q3IQF5"/>
    <dbReference type="STRING" id="348780.NP_3100A"/>
    <dbReference type="EnsemblBacteria" id="CAI49641">
        <property type="protein sequence ID" value="CAI49641"/>
        <property type="gene ID" value="NP_3100A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702898"/>
    <dbReference type="KEGG" id="nph:NP_3100A"/>
    <dbReference type="eggNOG" id="arCOG01678">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057642_0_0_2"/>
    <dbReference type="OrthoDB" id="204488at2157"/>
    <dbReference type="UniPathway" id="UPA00315">
        <property type="reaction ID" value="UER00080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004478">
        <property type="term" value="F:methionine adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.280">
        <property type="entry name" value="S-adenosylmethionine synthetase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00136">
        <property type="entry name" value="S_AdoMet_synth2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027790">
        <property type="entry name" value="AdoMet_synthase_2_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042544">
        <property type="entry name" value="AdoMet_synthase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002795">
        <property type="entry name" value="S-AdoMet_synthetase_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003364">
        <property type="entry name" value="PRK04439.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003366">
        <property type="entry name" value="PRK04439.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36697">
        <property type="entry name" value="S-ADENOSYLMETHIONINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36697:SF1">
        <property type="entry name" value="S-ADENOSYLMETHIONINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01941">
        <property type="entry name" value="AdoMet_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000259468" description="S-adenosylmethionine synthase">
        <location>
            <begin position="1"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="272"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="287"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="137"/>
            <end position="142"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="401" mass="43294" checksum="039FE5B76FEC6CB8" modified="2005-11-08" version="1">MSDRNIRVEPVVGRAVEEQDVEIVERKGLGHPDSLCDGIAEHVSQALARAYIDRVGKVLHYNTDETQLVAGTAAPAFGGGEVVDPIYLLITGRATKEYEGTKIPAETIALRAAREYINETLPFLEFGTDVVVDVKLGEGSGDLQEVFGEDGKQVPMSNDTSFGVGHAPLTETERIVLEAERALNGDYSDDNPAVGQDIKVMGKREGDDIDVTVAVAMVDRYVDDLDGYEAAVAGVREFVADLATDYTDRNVSVHVNTADDYDEGAIYLTTTGTSAEQGDDGSVGRGNRSNGLITPNRSMSMEATSGKNPVNHIGKIYNLLSTEIARTVVDEVDGIREIRIRLLSQIGQPIDKPHVADANLVTEDGIEIADIEDEVEAIIDAELENVTSITERVIDGELTTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IRF0</accession>
    <name>Q3IRF0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tubulin-like protein CetZ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cetZ1</name>
        <name evidence="6" type="synonym">cetZ</name>
        <name evidence="9" type="synonym">ftsZ4</name>
        <name evidence="9" type="ordered locus">NP_2404A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in cell shape control.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the CetZ family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322919.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IRF0"/>
    <dbReference type="STRING" id="348780.NP_2404A"/>
    <dbReference type="EnsemblBacteria" id="CAI49293">
        <property type="protein sequence ID" value="CAI49293"/>
        <property type="gene ID" value="NP_2404A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702730"/>
    <dbReference type="KEGG" id="nph:NP_2404A"/>
    <dbReference type="eggNOG" id="arCOG02202">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058152_0_0_2"/>
    <dbReference type="OrthoDB" id="329751at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02202">
        <property type="entry name" value="CetZ_tubulin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01946">
        <property type="entry name" value="CetZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032907">
        <property type="entry name" value="CetZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048737">
        <property type="entry name" value="CetZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045061">
        <property type="entry name" value="FtsZ/CetZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314">
        <property type="entry name" value="CELL DIVISION PROTEIN FTSZ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314:SF10">
        <property type="entry name" value="TUBULIN-LIKE PROTEIN CETZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21011">
        <property type="entry name" value="CetZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0133">Cell shape</keyword>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="8">
        <location>
            <begin position="2"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="365"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="365"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="379"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="10"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="110"/>
            <end position="112"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI49293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="392" mass="41919" checksum="24292E84B094F526" modified="2005-11-08" version="1">MKLAMIGFGQAGGKILDRFLEYDSTRGTGIVGHAVAVNSAKADLMGLDYVPNENRVLIGQSVVKGHGAGTEPELGERCAKEDMEEIQSAIDRMVSSEIDAFLVMAGLGGGTGSGGAPVLAEHLQRLYVEPVYGLGILPARDEGGIYNRNAARSFQRFAEAVDNLLTFDNDAFKTGGESLGEGYAEINAEIITRFGALFSAGEVEALGDNVAESVVDASEIINTLGEHGISSVGYASEPVQTKQQSSGLLDRLRGGNDKNGFSSGGDATNRITSLVRKATLGKLTLQCEVDSTERVLLLVSGPPEALNRKGIDKARKWLESQTDCMEVRAGDYPLPNEDKVAAIVVLSGVTDVPRVKEMQRMAVEAEEKVEEIRSQSKDDLDDLIEYGDDETQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IRL6</accession>
    <name>PYRH_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Uridylate kinase</fullName>
            <shortName evidence="1">UK</shortName>
            <ecNumber evidence="1">2.7.4.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Uridine monophosphate kinase</fullName>
            <shortName evidence="1">UMP kinase</shortName>
            <shortName evidence="1">UMPK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrH</name>
        <name type="ordered locus">NP_2272A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible phosphorylation of UMP to UDP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>UMP + ATP = UDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:24400"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.22"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Inhibited by UTP.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; UDP from UMP (UMPK route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UMP kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.22" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322854.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IRL6"/>
    <dbReference type="SMR" id="Q3IRL6"/>
    <dbReference type="STRING" id="348780.NP_2272A"/>
    <dbReference type="EnsemblBacteria" id="CAI49227">
        <property type="protein sequence ID" value="CAI49227"/>
        <property type="gene ID" value="NP_2272A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703087"/>
    <dbReference type="KEGG" id="nph:NP_2272A"/>
    <dbReference type="eggNOG" id="arCOG00858">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079546_0_0_2"/>
    <dbReference type="OrthoDB" id="372251at2157"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00275"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033862">
        <property type="term" value="F:UMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006225">
        <property type="term" value="P:UDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04253">
        <property type="entry name" value="AAK_UMPK-PyrH-Pf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01220_A">
        <property type="entry name" value="PyrH_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011817">
        <property type="entry name" value="Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011818">
        <property type="entry name" value="Uridylate_kinase_arch/spir"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02076">
        <property type="entry name" value="pyrH_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42833">
        <property type="entry name" value="URIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42833:SF4">
        <property type="entry name" value="URIDYLATE KINASE PUMPKIN, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005650">
        <property type="entry name" value="Uridylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000053964" description="Uridylate kinase">
        <location>
            <begin position="1"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="9"/>
            <end position="10"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="114"/>
            <end position="120"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01220"/>
        </source>
    </evidence>
    <sequence length="227" mass="23433" checksum="0892B3002F6A0287" modified="2005-11-08" version="1">MRIIVSIGGSVLAPDLEAGRVEAHADAIDSLVADGHEVAAVVGGGDVARQYIDSARDLGATEYDLDALGIDVTRLNARLLVTALNSSAIPEPAESHEDARASMRRGEVAVMGGTVPGHTTDAVAAMLAEMVEADLLIYATSVPGVFSADPNEDPSAERFDRLEASKLVDVISSIETTAGSNAPVDLLAAKVIERSGLRAIVLDGTAPERIADAVDGDPVGTEVLPNE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-01-10" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IRQ4</accession>
    <name>LEUC_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-isopropylmalate dehydratase large subunit</fullName>
            <ecNumber evidence="1">4.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Alpha-IPM isomerase</fullName>
            <shortName evidence="1">IPMI</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Isopropylmalate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">leuC</name>
        <name type="ordered locus">NP_2194A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate</text>
            <dbReference type="Rhea" id="RHEA:32287"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="EC" id="4.2.1.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of LeuC and LeuD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.33" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322816.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IRQ4"/>
    <dbReference type="SMR" id="Q3IRQ4"/>
    <dbReference type="STRING" id="348780.NP_2194A"/>
    <dbReference type="EnsemblBacteria" id="CAI49188">
        <property type="protein sequence ID" value="CAI49188"/>
        <property type="gene ID" value="NP_2194A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702880"/>
    <dbReference type="KEGG" id="nph:NP_2194A"/>
    <dbReference type="eggNOG" id="arCOG01698">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006714_3_4_2"/>
    <dbReference type="OrthoDB" id="6900at2157"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01583">
        <property type="entry name" value="IPMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01026">
        <property type="entry name" value="LeuC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004430">
        <property type="entry name" value="3-IsopropMal_deHydase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050067">
        <property type="entry name" value="IPM_dehydratase_rel_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033941">
        <property type="entry name" value="IPMI_cat"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00170">
        <property type="entry name" value="leuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004016">
        <property type="entry name" value="PRK05478.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009116">
        <property type="entry name" value="PRK12466.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43822:SF9">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43822">
        <property type="entry name" value="HOMOACONITASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0432">Leucine biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000076853" description="3-isopropylmalate dehydratase large subunit">
        <location>
            <begin position="1"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="293"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="348"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="408"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="411"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="474" mass="51242" checksum="C14F9F6F3AAD564D" modified="2005-11-08" version="1">MSEGTLYDKVWENHKVTTLPNGQDQLFVGLHLIHEVTSPQAFGMLRERDIEVARPDLTHATVDHIVPTADQSRPLDNEAAEEMMAELEENVREAGITLDDPTTGNQGIVHVIGPEQGLTQPGKTIVCGDSHTSTHGAFGALAFGIGTSQIRDVLATQTIAMEKKDVRKIEVTGELGEGVTAKDIILTIIRRLGTDGGVGYVYEYAGEAIENLGMEGRMSICNMSIEGGARAGYVNPDETTYEWLRETDAFADDPERFEELKPYWESVRSDPDAEYDDVVTIDGSEIEPVVTWGTTPGQGIGITEEIPAPEDLPADKQDTARRAQDHMGVEPGETMAGYNIDVAFLGSCTNARLPDLRSAAEIIKGREVHDSVRAMVVPGSQRVKAAAEDEGLDSVFKEAGFDWREAGCSMCLGMNDDQLVGDEACASSSNRNFVGRQGSKDGRTVLMSPEMVAAAAVTGEVTDVRELPKAEVEA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IRR4</accession>
    <name>CHEB_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein-glutamate methylesterase/protein-glutamine glutaminase</fullName>
            <ecNumber evidence="1">3.1.1.61</ecNumber>
            <ecNumber evidence="1">3.5.1.44</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cheB</name>
        <name type="ordered locus">NP_2174A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in chemotaxis. Part of a chemotaxis signal transduction system that modulates chemotaxis in response to various stimuli. Catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins or MCP) by CheR. Also mediates the irreversible deamidation of specific glutamine residues to glutamic acid.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-L-glutamate 5-O-methyl ester + H2O = L-glutamyl-[protein] + methanol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23236"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:10311"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:82795"/>
            <dbReference type="EC" id="3.1.1.61"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutaminyl-[protein] + H2O = L-glutamyl-[protein] + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16441"/>
            <dbReference type="Rhea" id="RHEA-COMP:10207"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="EC" id="3.5.1.44"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">Contains a C-terminal catalytic domain, and an N-terminal region which modulates catalytic activity.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Phosphorylated by CheA. Phosphorylation of the N-terminal regulatory domain activates the methylesterase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CheB family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.61" evidence="1"/>
    <dbReference type="EC" id="3.5.1.44" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322806.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IRR4"/>
    <dbReference type="SMR" id="Q3IRR4"/>
    <dbReference type="STRING" id="348780.NP_2174A"/>
    <dbReference type="EnsemblBacteria" id="CAI49178">
        <property type="protein sequence ID" value="CAI49178"/>
        <property type="gene ID" value="NP_2174A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702598"/>
    <dbReference type="KEGG" id="nph:NP_2174A"/>
    <dbReference type="eggNOG" id="arCOG02382">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_51_0_2"/>
    <dbReference type="OrthoDB" id="2857at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000156">
        <property type="term" value="F:phosphorelay response regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008984">
        <property type="term" value="F:protein-glutamate methylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050568">
        <property type="term" value="F:protein-glutamine glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16432">
        <property type="entry name" value="CheB_Rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17541">
        <property type="entry name" value="REC_CheB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.180">
        <property type="entry name" value="Methylesterase CheB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00099">
        <property type="entry name" value="CheB_chemtxs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008248">
        <property type="entry name" value="CheB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035909">
        <property type="entry name" value="CheB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000673">
        <property type="entry name" value="Sig_transdc_resp-reg_Me-estase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001965">
        <property type="entry name" value="PRK00742.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42872">
        <property type="entry name" value="PROTEIN-GLUTAMATE METHYLESTERASE/PROTEIN-GLUTAMINE GLUTAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42872:SF6">
        <property type="entry name" value="PROTEIN-GLUTAMATE METHYLESTERASE_PROTEIN-GLUTAMINE GLUTAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01339">
        <property type="entry name" value="CheB_methylest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000876">
        <property type="entry name" value="RR_chemtxs_CheB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52738">
        <property type="entry name" value="Methylesterase CheB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50122">
        <property type="entry name" value="CHEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0145">Chemotaxis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000225498" description="Protein-glutamate methylesterase/protein-glutamine glutaminase">
        <location>
            <begin position="1"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="1">
        <location>
            <begin position="7"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="CheB-type methylesterase" evidence="1">
        <location>
            <begin position="161"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="200"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="297"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="355" mass="36777" checksum="4BF33920E0061EFA" modified="2005-11-08" version="1">MSNNGIAAVVVDDSQFMRTVISDMLDDAGITVVATASNGESGVEAVRTHEPDVVTMDLKMPGMDGIEATGRIMDECPTPVLVLSAHADDGAELTFEAMDEGAVDFFKKPSGEVSVGIKQQQDELVEKVRSVAGADVSATEAAAERTTSTATSTTTSRSASEYVDKPTLVIGSSTGGPTVVEQLLSELPLDADLRILVVQHMPDGFTGRFAKRLDGASEYSVSEATGGERIGGGEALVAQGGYHLEVSGYGGGRLRVTLTEDEPVNNVRPAVDVTLQTAAEQVDGPLTAAILTGMGADGADGVESVSAAGGSVVAQDEETSAVFGMPQRAIETGVVDDVRPRNELADGVLDTIMRE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ISN0</accession>
    <name>PURA_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Adenylosuccinate synthetase</fullName>
            <shortName evidence="1">AMPSase</shortName>
            <shortName evidence="1">AdSS</shortName>
            <ecNumber evidence="1">6.3.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">IMP--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purA</name>
        <name type="ordered locus">NP_1530A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>IMP + L-aspartate + GTP = N(6)-(1,2-dicarboxyethyl)-AMP + GDP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15753"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="6.3.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the adenylosuccinate synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.4" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322490.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ISN0"/>
    <dbReference type="SMR" id="Q3ISN0"/>
    <dbReference type="STRING" id="348780.NP_1530A"/>
    <dbReference type="EnsemblBacteria" id="CAI48856">
        <property type="protein sequence ID" value="CAI48856"/>
        <property type="gene ID" value="NP_1530A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703066"/>
    <dbReference type="KEGG" id="nph:NP_1530A"/>
    <dbReference type="eggNOG" id="arCOG04387">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029848_0_2_2"/>
    <dbReference type="OrthoDB" id="372247at2157"/>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00335"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004019">
        <property type="term" value="F:adenylosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046040">
        <property type="term" value="P:IMP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03108">
        <property type="entry name" value="AdSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.300.10:FF:000001">
        <property type="entry name" value="Adenylosuccinate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.170.10:FF:000001">
        <property type="entry name" value="Adenylosuccinate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.440.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.300.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.170.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00011">
        <property type="entry name" value="Adenylosucc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018220">
        <property type="entry name" value="Adenylosuccin_syn_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033128">
        <property type="entry name" value="Adenylosuccin_syn_Lys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042109">
        <property type="entry name" value="Adenylosuccinate_synth_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042110">
        <property type="entry name" value="Adenylosuccinate_synth_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042111">
        <property type="entry name" value="Adenylosuccinate_synth_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001114">
        <property type="entry name" value="Adenylosuccinate_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002223">
        <property type="entry name" value="PRK01117.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010357">
        <property type="entry name" value="PRK13785.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00184">
        <property type="entry name" value="purA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846">
        <property type="entry name" value="ADENYLOSUCCINATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846:SF0">
        <property type="entry name" value="ADENYLOSUCCINATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00709">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00788">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01266">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00513">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000224342" description="Adenylosuccinate synthetase">
        <location>
            <begin position="1"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="40"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="11"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="12"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="37"/>
            <end position="40"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="39"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="324"/>
            <end position="330"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="356"/>
            <end position="358"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="441"/>
            <end position="443"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00011"/>
        </source>
    </evidence>
    <sequence length="456" mass="48910" checksum="6E25E0DDD886B32B" modified="2005-11-08" version="1">MTVTIVGSQLGDEGKGGIVDVYGDAVDVVVRYQGGDNAGHTVVHEGDEYKLSLVPSGAVRGKVGVLGNGCVVNPETLFEELDALRERGLTPDVRVAERAHAILPYHRVLDGIEEDVKSDDDLAAGTTGRGIGPTYEDKAGRRGVRIGDLLDAETLRARLEYVVPQKRALVEDVYNLEIGDDIDADAFDVEALFEQYREFGRRLDEEGMTVNCGEFLNEHLDNGDEIMFEGAQGTSIDIDHGIYPYVTSSNPTAGAAAVGTGVGPTVVGRGEVVGIVKAYLSRVGTGPLPTELGSVDGQTPNNGGRPDESDLATYIRDEGGEYGTVTGRPRRVGWLDMPMLRHAARANGFTGLAVNHLDVLAGLEEVKVGHAYTLDGEQLLTMPATTEQWADCEAEFRSFDGWPDVDWGAVADEGYEALPENARTYLDYIADELDAPIYAVGVGPGREETVVVESPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ISV1</accession>
    <name>Q3ISV1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">PhoU domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">phoU5</name>
        <name evidence="3" type="ordered locus">NP_1386A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48784.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939460.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ISV1"/>
    <dbReference type="STRING" id="348780.NP_1386A"/>
    <dbReference type="EnsemblBacteria" id="CAI48784">
        <property type="protein sequence ID" value="CAI48784"/>
        <property type="gene ID" value="NP_1386A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703030"/>
    <dbReference type="KEGG" id="nph:NP_1386A"/>
    <dbReference type="eggNOG" id="arCOG00318">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069302_1_0_2"/>
    <dbReference type="OrthoDB" id="298081at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:intracellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045936">
        <property type="term" value="P:negative regulation of phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="entry name" value="Phosphate transport system protein phou homolog 2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028366">
        <property type="entry name" value="PhoU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026022">
        <property type="entry name" value="PhoU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930:SF6">
        <property type="entry name" value="PHOSPHATE REGULATORY PROTEIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930">
        <property type="entry name" value="PHOSPHATE-SPECIFIC TRANSPORT SYSTEM ACCESSORY PROTEIN PHOU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04014">
        <property type="entry name" value="MazE_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01895">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109755">
        <property type="entry name" value="PhoU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="2">
        <location>
            <begin position="7"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="PhoU" evidence="1">
        <location>
            <begin position="142"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48784.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="344" mass="38208" checksum="C3DA80F399E00EC8" modified="2012-10-31" version="2">METRKVQRLGPSTLAMTLPAEWASEQNVEKGDEVSLRMGSKGTLTVMPESVQQEESEAVIHAQSLDADAVERAIVAQYVLGRRIIHVEVEEGGTLDSAQINAVYNAETQLMGLGVIEETPERIAIRCSVDPEDFTLDNLLERLESTGSTMRNEGVKALAHGNPDLAQRALNRERQANKIFVLLLRLIFTAYQNPNLARAVGLSSGFPLIGYRSIAKNLELTADNAEDIAEIVLETEGHTLNVDQKTMRRIREFTDDVNEITELGVQAAVTRDYDLAIETRDAFADIRNREVEILDDLDEMPNEDLLRVREVLVSLGQTAQYAVRNAEIATNLALNEESDHVTIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-08-21" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ITE8</accession>
    <name>CDPAS_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CDP-archaeol synthase</fullName>
            <ecNumber evidence="1">2.7.7.67</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">carS</name>
        <name type="ordered locus">NP_0990A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol (CDP-archaeol) from 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate (DGGGP) and CTP. This reaction is the third ether-bond-formation step in the biosynthesis of archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phosphate + CTP + H(+) = CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:25690"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:58837"/>
            <dbReference type="ChEBI" id="CHEBI:58838"/>
            <dbReference type="EC" id="2.7.7.67"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDP-archaeol synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.67" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322221.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ITE8"/>
    <dbReference type="SMR" id="Q3ITE8"/>
    <dbReference type="STRING" id="348780.NP_0990A"/>
    <dbReference type="EnsemblBacteria" id="CAI48586">
        <property type="protein sequence ID" value="CAI48586"/>
        <property type="gene ID" value="NP_0990A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702595"/>
    <dbReference type="KEGG" id="nph:NP_0990A"/>
    <dbReference type="eggNOG" id="arCOG04106">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105710_0_0_2"/>
    <dbReference type="OrthoDB" id="45383at2157"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043338">
        <property type="term" value="F:CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01117">
        <property type="entry name" value="CDP_archaeol_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032690">
        <property type="entry name" value="CarS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002726">
        <property type="entry name" value="CarS_archaea"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003114">
        <property type="entry name" value="PRK04032.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39650">
        <property type="entry name" value="CDP-ARCHAEOL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39650:SF1">
        <property type="entry name" value="CDP-ARCHAEOL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01864">
        <property type="entry name" value="CarS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000298283" description="CDP-archaeol synthase">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01117"/>
        </source>
    </evidence>
    <sequence length="189" mass="19794" checksum="C1937BC86CF5DFC9" modified="2005-11-08" version="1">MVAETVAIAVWAMLPAYVPNNVAVLAGGGRPIDGGRTLDKLTDEDGVLYDENRILGDGKTWRGTAFGTAAGVVLALLLNQLQPFVAGTVGVPQFPIAAAVALAFGAMLGDILASFLKRRTGRQRGAAFPGVDQLDFVIVSLALTAIVATGWFLATFTLPVLVAIFVLTPVLHVSTNGLAYAFGLKDEPW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ITS0</accession>
    <name>UVRB_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UvrABC system protein B</fullName>
            <shortName evidence="1">Protein UvrB</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Excinuclease ABC subunit B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">uvrB</name>
        <name type="ordered locus">NP_0744A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA(2)B(2) complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">The beta-hairpin motif is involved in DNA binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UvrB family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322099.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ITS0"/>
    <dbReference type="SMR" id="Q3ITS0"/>
    <dbReference type="STRING" id="348780.NP_0744A"/>
    <dbReference type="EnsemblBacteria" id="CAI48463">
        <property type="protein sequence ID" value="CAI48463"/>
        <property type="gene ID" value="NP_0744A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703458"/>
    <dbReference type="KEGG" id="nph:NP_0744A"/>
    <dbReference type="eggNOG" id="arCOG04748">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009621_2_1_2"/>
    <dbReference type="OrthoDB" id="8371at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009380">
        <property type="term" value="C:excinuclease repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009381">
        <property type="term" value="F:excinuclease ABC activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120545">
        <property type="term" value="F:nucleic acid conformation isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd17916">
        <property type="entry name" value="DEXHc_UvrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18790">
        <property type="entry name" value="SF2_C_UvrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.10">
        <property type="entry name" value="UVR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00204">
        <property type="entry name" value="UvrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001943">
        <property type="entry name" value="UVR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036876">
        <property type="entry name" value="UVR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004807">
        <property type="entry name" value="UvrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041471">
        <property type="entry name" value="UvrB_inter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024759">
        <property type="entry name" value="UvrB_YAD/RRR_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003673">
        <property type="entry name" value="PRK05298.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00631">
        <property type="entry name" value="uvrb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24029">
        <property type="entry name" value="UVRABC SYSTEM PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24029:SF0">
        <property type="entry name" value="UVRABC SYSTEM PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12344">
        <property type="entry name" value="UvrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17757">
        <property type="entry name" value="UvrB_inter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46600">
        <property type="entry name" value="C-terminal UvrC-binding domain of UvrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0228">DNA excision</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0267">Excision nuclease</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0742">SOS response</keyword>
    <feature type="chain" id="PRO_0000227389" description="UvrABC system protein B">
        <location>
            <begin position="1"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="1">
        <location>
            <begin position="39"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="1">
        <location>
            <begin position="442"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="domain" description="UVR" evidence="1">
        <location>
            <begin position="630"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="587"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Beta-hairpin">
        <location>
            <begin position="105"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="587"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="604"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="52"/>
            <end position="59"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="683" mass="77602" checksum="DD7999D014477A14" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IUQ6</accession>
    <name>SYR_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Arginine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Arginyl-tRNA synthetase</fullName>
            <shortName evidence="1">ArgRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argS</name>
        <name type="ordered locus">NP_0066A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Arg) + L-arginine + ATP = L-arginyl-tRNA(Arg) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20301"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.19"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.19" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321763.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IUQ6"/>
    <dbReference type="SMR" id="Q3IUQ6"/>
    <dbReference type="STRING" id="348780.NP_0066A"/>
    <dbReference type="EnsemblBacteria" id="CAI48124">
        <property type="protein sequence ID" value="CAI48124"/>
        <property type="gene ID" value="NP_0066A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702524"/>
    <dbReference type="KEGG" id="nph:NP_0066A"/>
    <dbReference type="eggNOG" id="arCOG00487">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006406_6_1_2"/>
    <dbReference type="OrthoDB" id="372102at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004814">
        <property type="term" value="F:arginine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006420">
        <property type="term" value="P:arginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000006">
        <property type="entry name" value="Arginyl-tRNA synthetase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.70">
        <property type="entry name" value="Arginyl tRNA synthetase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00123">
        <property type="entry name" value="Arg_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001278">
        <property type="entry name" value="Arg-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005148">
        <property type="entry name" value="Arg-tRNA-synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036695">
        <property type="entry name" value="Arg-tRNA-synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035684">
        <property type="entry name" value="ArgRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008909">
        <property type="entry name" value="DALR_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00456">
        <property type="entry name" value="argS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956:SF5">
        <property type="entry name" value="ARGININE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956">
        <property type="entry name" value="ARGINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03485">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05746">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00750">
        <property type="entry name" value="tRNA-synt_1d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01038">
        <property type="entry name" value="TRNASYNTHARG"/>
    </dbReference>
    <dbReference type="SMART" id="SM01016">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00836">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55190">
        <property type="entry name" value="Arginyl-tRNA synthetase (ArgRS), N-terminal 'additional' domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000242133" description="Arginine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="123"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00123"/>
        </source>
    </evidence>
    <sequence length="580" mass="64494" checksum="669BFEFF05D103AD" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETA1</accession>
    <name>A0A1U7ETA1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Halocyanin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">hcp4</name>
        <name evidence="11" type="ordered locus">NP_0050A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="7">Binds 1 copper ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321755.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETA1"/>
    <dbReference type="STRING" id="348780.NP_0050A"/>
    <dbReference type="EnsemblBacteria" id="CAI48116">
        <property type="protein sequence ID" value="CAI48116"/>
        <property type="gene ID" value="NP_0050A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702803"/>
    <dbReference type="KEGG" id="nph:NP_0050A"/>
    <dbReference type="eggNOG" id="arCOG02921">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1154371_0_0_2"/>
    <dbReference type="OrthoDB" id="11088at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04220">
        <property type="entry name" value="Halocyanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002386">
        <property type="entry name" value="Amicyanin/Pseudoazurin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000923">
        <property type="entry name" value="BlueCu_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028871">
        <property type="entry name" value="BlueCu_1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017533">
        <property type="entry name" value="Halocyanin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03102">
        <property type="entry name" value="halo_cynanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00127">
        <property type="entry name" value="Copper-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00155">
        <property type="entry name" value="AMICYANIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00196">
        <property type="entry name" value="COPPER_BLUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0186">Copper</keyword>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0574">Periplasm</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Blue (type 1) copper" evidence="10">
        <location>
            <begin position="66"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="171"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602386-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI48116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="223" mass="23341" checksum="8F81C7AFA17DE477" modified="2017-05-10" version="1">MAPNDGTGLGRRDLFRATAGAAAAAAATGTASTAYAQETFDYDGWFEDVPNYDGTVDMTGEDEVVVDVGGGAAGLTFEPAAIHVDPGTTVVYEWTGEGGGHNVVEQETGDRYESERTDEAGTIYEVTYESDGISKYVCVPHIANGMKGAVAVGDGEGVPDITEGETLIGGPEEEDDDEPEDLPPAFEMDSEVFAMFGVAGLLALLSPLGVLYLMRRQNGGEPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETB4</accession>
    <name>A0A1U7ETB4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UspA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0056A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321758.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETB4"/>
    <dbReference type="STRING" id="348780.NP_0056A"/>
    <dbReference type="EnsemblBacteria" id="CAI48119">
        <property type="protein sequence ID" value="CAI48119"/>
        <property type="gene ID" value="NP_0056A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700747"/>
    <dbReference type="KEGG" id="nph:NP_0056A"/>
    <dbReference type="eggNOG" id="arCOG02053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_11_1_2"/>
    <dbReference type="OrthoDB" id="105697at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF6">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN UP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006276">
        <property type="entry name" value="UspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="144" mass="15447" checksum="98E523BE4AA32D61" modified="2017-05-10" version="1">MYDDILVPTDGGEQMEEVIDQAVAIAADRGATLHGISVIDKGVFLTLDEHLKDAVEEELTTEAQEAVDELSAAADDADLESEAVVRRGRPGEEIRAYADEVGADLIVMGTRGADSHERRMLGSVSQDVVTESTLPVLVIPLEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETC9</accession>
    <name>A0A1U7ETC9_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable transcription termination protein NusA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">nusA</name>
        <name evidence="8" type="ordered locus">NP_0118A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Participates in transcription termination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NusA family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321789.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETC9"/>
    <dbReference type="STRING" id="348780.NP_0118A"/>
    <dbReference type="EnsemblBacteria" id="CAI48150">
        <property type="protein sequence ID" value="CAI48150"/>
        <property type="gene ID" value="NP_0118A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703000"/>
    <dbReference type="KEGG" id="nph:NP_0118A"/>
    <dbReference type="eggNOG" id="arCOG01760">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131906_0_0_2"/>
    <dbReference type="OrthoDB" id="4116at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22530">
        <property type="entry name" value="KH-II_NusA_arch_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22531">
        <property type="entry name" value="KH-II_NusA_arch_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00945_A">
        <property type="entry name" value="NusA_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058582">
        <property type="entry name" value="KH_NusA_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010212">
        <property type="entry name" value="NusA_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030842">
        <property type="entry name" value="TF_NusA_bacterial"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01952">
        <property type="entry name" value="nusA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22648">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR NUSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22648:SF0">
        <property type="entry name" value="TRANSCRIPTION TERMINATION_ANTITERMINATION PROTEIN NUSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26594">
        <property type="entry name" value="KH_NusA_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="Prokaryotic type KH domain (KH-domain type II)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 6" id="KW-0806">Transcription termination</keyword>
    <feature type="domain" description="NusA-like second KH" evidence="7">
        <location>
            <begin position="77"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF26594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI48150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="141" mass="15031" checksum="1294AF09BA58ACCE" modified="2017-05-10" version="1">MAVTLTDEARRYIALFDEETDVAATDCVVDEEYDRIAFVVPPGTMGQAIGPGGDHVATVESKVGRDVTVIEGAETAADFVANALAPAAVYNVTISENDTTVAYAEVDENDRGVAIGADGRNIDLARRLADRHFDIDDVELT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETF6</accession>
    <name>A0A1U7ETF6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Signal recognition particle receptor FtsY</fullName>
            <shortName evidence="8">SRP receptor</shortName>
            <ecNumber evidence="8">3.6.5.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 11" type="primary">ftsY</name>
        <name evidence="11" type="ordered locus">NP_0182A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Acts as a receptor for the complex formed by the signal recognition particle (SRP) and the ribosome-nascent chain (RNC).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.4"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Peripheral membrane protein</topology>
            <orientation evidence="8">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the GTP-binding SRP family. FtsY subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.4" evidence="8"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321821.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETF6"/>
    <dbReference type="STRING" id="348780.NP_0182A"/>
    <dbReference type="EnsemblBacteria" id="CAI48182">
        <property type="protein sequence ID" value="CAI48182"/>
        <property type="gene ID" value="NP_0182A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702726"/>
    <dbReference type="KEGG" id="nph:NP_0182A"/>
    <dbReference type="eggNOG" id="arCOG01227">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009301_3_1_2"/>
    <dbReference type="OrthoDB" id="372188at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005047">
        <property type="term" value="F:signal recognition particle binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000566">
        <property type="entry name" value="Signal recognition particle receptor subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="entry name" value="Signal recognition particle SRP54, nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00920">
        <property type="entry name" value="FtsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004390">
        <property type="entry name" value="SR_rcpt_FtsY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00064">
        <property type="entry name" value="ftsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43134">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43134:SF1">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="Domain of the SRP/SRP receptor G-proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRP54-type proteins GTP-binding" evidence="10">
        <location>
            <begin position="340"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="17"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="51"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="179"/>
            <end position="186"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="261"/>
            <end position="265"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="319"/>
            <end position="322"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI48182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="368" mass="39441" checksum="65D4EE889999C6BC" modified="2017-05-10" version="1">MFEGLKDKLSSFKGDVEEQADEPDSEADAAEPETADAEAAAEPEASSTDTDTGEPDSVTEPEPDSDPETDAEDDPGLAKKAALAATGRTIITEEELEEPLEQLELELLQGDVEMSVAQEITDRIREELVGETRKQVESGEQVVERAIAEALRDVISVGQFDFESRIADAEKPVTIVFTGVNGVGKTTSIAKLARWFEERGHSSVLANGDTYRAGANEQIEEHADALDKKIITHEQGGDPAAVIYDAVEYAEANDVDVVLGDTAGRLHTADDLMAQLEKIDRVVDPDLTLFVDEAVAGQDATNRAKEFNDAAEIDGAILTKADADSQGGAAISIAHVTGKPILFLGTGQGYDDIERFDPDEIVSRLLEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETJ0</accession>
    <name>A0A1U7ETJ0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Homolog to diacyglycerol O-acyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">NP_0266A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>an acyl-CoA + a 1,2-diacyl-sn-glycerol = a triacyl-sn-glycerol + CoA</text>
            <dbReference type="Rhea" id="RHEA:10868"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:64615"/>
            <dbReference type="EC" id="2.3.1.20"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Glycerolipid metabolism; triacylglycerol biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321863.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETJ0"/>
    <dbReference type="STRING" id="348780.NP_0266A"/>
    <dbReference type="DNASU" id="3700807"/>
    <dbReference type="EnsemblBacteria" id="CAI48224">
        <property type="protein sequence ID" value="CAI48224"/>
        <property type="gene ID" value="NP_0266A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700807"/>
    <dbReference type="KEGG" id="nph:NP_0266A"/>
    <dbReference type="eggNOG" id="arCOG10773">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024186_3_1_2"/>
    <dbReference type="OrthoDB" id="236193at2157"/>
    <dbReference type="UniPathway" id="UPA00282"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004144">
        <property type="term" value="F:diacylglycerol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014292">
        <property type="entry name" value="Acyl_transf_WS/DGAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045034">
        <property type="entry name" value="O-acyltransferase_WSD1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009721">
        <property type="entry name" value="O-acyltransferase_WSD1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004255">
        <property type="entry name" value="O-acyltransferase_WSD1_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02946">
        <property type="entry name" value="acyl_WS_DGAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31650">
        <property type="entry name" value="O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31650:SF1">
        <property type="entry name" value="WAX ESTER SYNTHASE_DIACYLGLYCEROL ACYLTRANSFERASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06974">
        <property type="entry name" value="WS_DGAT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03007">
        <property type="entry name" value="WS_DGAT_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-acyltransferase WSD1-like N-terminal" evidence="9">
        <location>
            <begin position="7"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="O-acyltransferase WSD1 C-terminal" evidence="10">
        <location>
            <begin position="327"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="164"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF06974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI48224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="473" mass="51186" checksum="7C67AFD9F5F3277F" modified="2017-05-10" version="1">MSERESLSGADNAWRRMGTPTNLMTITGVMMFDERMTYEDLCNRLEERLLRFERFQQRIGGRKRRFRQPYWETVDGFDVEPHVYHISLPEPQDKATFEAFVGKLMSRPLDESRPLWEAYLVDGAGPGEGNAVAFRLNHSIGDGFALLYVLLGLVDNPGDIELPSGMVSAPDDIGDDADSGADGGNGSDESDVASGDGQPADTDSKSDGERGSSGLPSPVSAAGTAATGVATGYDLLFGDDDPDTSLRGELGQTKRAAWTDEIPLEQVRTVCEAQPHRATINDVLLAALAGALRRLLEDRGEPVDGMELHGTVPVNLKPMAERDGSLGNNFGLVFLPLPVGTEPLQERISIIHEWMDGKRAGIQAFLMYALLTIGGHVPEYVQKKVMTLFEDRATGVVTNVPGPTDAIQFAGREITDMIFWVPQANEQGIGISIFSYDGNVRVGVAADENLLGAPGELAAAFEAEMEALLEDAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETL1</accession>
    <name>A0A1U7ETL1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DUF309 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0312A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321885.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETL1"/>
    <dbReference type="STRING" id="348780.NP_0312A"/>
    <dbReference type="EnsemblBacteria" id="CAI48247">
        <property type="protein sequence ID" value="CAI48247"/>
        <property type="gene ID" value="NP_0312A"/>
    </dbReference>
    <dbReference type="GeneID" id="28378307"/>
    <dbReference type="KEGG" id="nph:NP_0312A"/>
    <dbReference type="eggNOG" id="arCOG09205">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116128_0_0_2"/>
    <dbReference type="OrthoDB" id="270022at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3450.10">
        <property type="entry name" value="TTHA0068-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005500">
        <property type="entry name" value="DUF309"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023203">
        <property type="entry name" value="TTHA0068_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03745">
        <property type="entry name" value="DUF309"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140663">
        <property type="entry name" value="TTHA0068-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="194" mass="20738" checksum="13F0BEE4319425CD" modified="2017-05-10" version="1">MEALRAGLALYNAGHFLAAHEPWEDRWLDAPPESRSDCLQGLIQATAAIHKARTGNWSGAVGLAESAVDYLADCDRVAVGSLVDWLARLAADPELAERERPPQLRLDGNVVAVSSLRVPALASAAAALAAVHGDDFVDTALTYAAADLDSEATSPLLAPLRAYVAEGTPIAATRLERRVEKRTRRDDDVEGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETN9</accession>
    <name>A0A1U7ETN9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">HTH domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0386A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321922.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETN9"/>
    <dbReference type="STRING" id="348780.NP_0386A"/>
    <dbReference type="EnsemblBacteria" id="CAI48284">
        <property type="protein sequence ID" value="CAI48284"/>
        <property type="gene ID" value="NP_0386A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700838"/>
    <dbReference type="KEGG" id="nph:NP_0386A"/>
    <dbReference type="eggNOG" id="arCOG04479">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_162441_0_0_2"/>
    <dbReference type="OrthoDB" id="30924at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038767">
        <property type="entry name" value="PF0610-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049159">
        <property type="entry name" value="PF0610-like_wHTH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057022">
        <property type="entry name" value="PF0610-like_Zn_ribbon_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40663">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40663:SF2">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21476">
        <property type="entry name" value="PF0610-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23470">
        <property type="entry name" value="Zn_ribbon_PF0610"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PF0610-like winged HTH N-terminal" evidence="1">
        <location>
            <begin position="7"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="PF0610-like rubredoxin-like zinc beta-ribbon C-terminal" evidence="2">
        <location>
            <begin position="59"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="96" mass="10688" checksum="27C2D933B5918BE2" modified="2017-05-10" version="1">MSTDDRTTREKIRAHLRKEEAEAGALAAEFGVTVEEALSHVEHIARSLESADEELLVAPPACRSCGFEDFDDLINRPSRCPECKSENLSEPVFTVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETQ4</accession>
    <name>A0A1U7ETQ4_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycerophosphoryl diester phosphodiesterase membrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0410A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321934.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETQ4"/>
    <dbReference type="STRING" id="348780.NP_0410A"/>
    <dbReference type="EnsemblBacteria" id="CAI48296">
        <property type="protein sequence ID" value="CAI48296"/>
        <property type="gene ID" value="NP_0410A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700847"/>
    <dbReference type="KEGG" id="nph:NP_0410A"/>
    <dbReference type="eggNOG" id="arCOG04706">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051458_0_0_2"/>
    <dbReference type="OrthoDB" id="137652at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055966">
        <property type="entry name" value="DUF7544"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24400">
        <property type="entry name" value="DUF7544"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="259"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="318" mass="33586" checksum="FED71883086357DC" modified="2017-05-10" version="1">MALHALEAIDDALTATLRYRPQGLREWVWVGIVATAVASPGIGLSVGSNPDIPEESSDQLNELLAVATGEAAIAAVAALAAVWALFVIAGAFLEFPFIRWLRDGEAAIAEETRQRWRQALGLAVFRLVLQAVPVATIGWLVVSAVGDGAQPIAYVLAVSDYWLWLLATGTLVGVVGALTTAFVVPTMALSDRGVLGAWRRFWPTLTGSLTEFLAYAVAATVFAYIGGLMVVVAAIVTFVVVAIPGLLLAMVAPMLSVTVLAVGGGALMTVAVIAVYAIVQMYLRYYALFVLGYVDGDLDYAAERRQAVRNGESSADEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETR2</accession>
    <name>A0A1U7ETR2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF5611 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0430A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321942.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETR2"/>
    <dbReference type="STRING" id="348780.NP_0430A"/>
    <dbReference type="EnsemblBacteria" id="CAI48306">
        <property type="protein sequence ID" value="CAI48306"/>
        <property type="gene ID" value="NP_0430A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700853"/>
    <dbReference type="KEGG" id="nph:NP_0430A"/>
    <dbReference type="eggNOG" id="arCOG04690">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139544_0_0_2"/>
    <dbReference type="OrthoDB" id="192476at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040713">
        <property type="entry name" value="DUF5611"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18446">
        <property type="entry name" value="DUF5611"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5611" evidence="2">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="130" mass="14734" checksum="2A14412E7E6E3ACC" modified="2017-05-10" version="1">MEEYKMRRGEYLEERVPDMEATIEEYFGPITGTEEYKGNDLHVVADPDNPVFNRVVAGTMSYSSKKDKLAVDFDERELEELMETGDVDAAADANNAKNDFLLECTGRDAKARRESMKRAVEDDAETPDNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETS8</accession>
    <name>A0A1U7ETS8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Major facilitator superfamily transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tp14</name>
        <name evidence="7" type="ordered locus">NP_0500A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48341.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETS8"/>
    <dbReference type="STRING" id="348780.NP_0500A"/>
    <dbReference type="EnsemblBacteria" id="CAI48341">
        <property type="protein sequence ID" value="CAI48341"/>
        <property type="gene ID" value="NP_0500A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_0500A"/>
    <dbReference type="eggNOG" id="arCOG00130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_62_1_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061513">
        <property type="term" value="F:glucose 6-phosphate:phosphate antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051337">
        <property type="entry name" value="OPA_Antiporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43826">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE EXCHANGER SLC37A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43826:SF3">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE EXCHANGER SLC37A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="224"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="262"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="301"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="325"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="360"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="403"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="8"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48341.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="430" mass="44394" checksum="D7B9BE10653D7326" modified="2017-05-10" version="1">MDPQRRRWIAFLALAGTYALVSVYRLSTAVLADELTRAFSVTGTQLGNLHASFFYVYAAMQLPTGVLADRLGARRTVTAGALVMSLGGLAFAGAESYAVAFVGRALIGLGGSVLFIAILRFCANWFRPSEFARMSGITIAVSGFGGILATTPLAVAVAAAGWRRTLAGLSVLGIGLAVAAYIVARDSPVDAGLEPMSGVDTPGASSLRDVLANARTVLGEVETWLCGAVLFAGTGINITVVGLWGIPYLVQTYGFSVTTASTYTLLGSAGLLVGPPVVGTVSDRLSRRRDTEAGTGGRTRLIVVGMALYAAAFALLAVTGAPPTAVVAVVFFASGGLAGAYALTYAVVKERHDASASGVSTGTINMMAFSGAAVLPTAMGYALDAYWTGETIGGARVYTEFGYQVAFGLTALVAFGAFLSAVWLHYRVSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETV0</accession>
    <name>A0A1U7ETV0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable secreted glycoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0528A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321991.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETV0"/>
    <dbReference type="STRING" id="348780.NP_0528A"/>
    <dbReference type="EnsemblBacteria" id="CAI48355">
        <property type="protein sequence ID" value="CAI48355"/>
        <property type="gene ID" value="NP_0528A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700887"/>
    <dbReference type="KEGG" id="nph:NP_0528A"/>
    <dbReference type="eggNOG" id="arCOG02417">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_887445_0_0_2"/>
    <dbReference type="OrthoDB" id="385920at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="313" mass="32632" checksum="FFCDA5B4529FCCD5" modified="2017-05-10" version="1">MRRRTVLALAGAAGTAGLAGCLDDVLGSENGDEPEPEDDPDDGDSSGEGNQPEDDEEQTGDESEDDRDRSPEETVVAFYEAIADDDVERANELRHSDGIGPFEEEDLDGEPTVEETELVESDDETATVEALVVPDGAEGSVVSLVTLEREDGRWRIVAIGSPQEGDKTELTPNAAFETERENGSRQIIHTAGDHIKAANLHIRGDGIEETGSWTDLGGRASGTVGDEPAVTAGDSVTVGISDDYSISVVWEDGDTAVTLMEMAGASDSATESGDDGAESGNGGESDSDEANGDGETAESDTDSDEESGASDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETW9</accession>
    <name>A0A1U7ETW9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SIMPL domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0580A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322017.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETW9"/>
    <dbReference type="STRING" id="348780.NP_0580A"/>
    <dbReference type="EnsemblBacteria" id="CAI48381">
        <property type="protein sequence ID" value="CAI48381"/>
        <property type="gene ID" value="NP_0580A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700895"/>
    <dbReference type="KEGG" id="nph:NP_0580A"/>
    <dbReference type="eggNOG" id="arCOG04715">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080344_2_0_2"/>
    <dbReference type="OrthoDB" id="12132at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.170">
        <property type="entry name" value="Protein of unknown function (DUF541), domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2970">
        <property type="entry name" value="Protein of unknown function (DUF541), domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052022">
        <property type="entry name" value="26kDa_periplasmic_antigen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007497">
        <property type="entry name" value="SIMPL/DUF541"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34387">
        <property type="entry name" value="SLR1258 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34387:SF2">
        <property type="entry name" value="SLR1258 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04402">
        <property type="entry name" value="SIMPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="248" mass="26081" checksum="2AF04EEBAD49094D" modified="2017-05-10" version="1">MQRDTLSILIVTASLLAMALVGAAVVSPIGADDPASDADRTITVDASGGADTEPDRGVVSTAAVAEGDDPAAVRDELNERADALRTNLEAAGLTDDDYETTEYRINEPRRPPREEGTDEPDYRGTHSFTVDLDDPDRIGTVIDAAADADAEVGDIEFTLSETVRDELREDAIDNAMGDARVQADSIADNGALQVTGVAHVDATQRSFSTVSYEVAATPAPEDDAATDVDTGDVSVTYDVEVTYNATAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU12</accession>
    <name>A0A1U7EU12_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">PaaI family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_0664A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioesterase PaaI family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322059.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU12"/>
    <dbReference type="STRING" id="348780.NP_0664A"/>
    <dbReference type="EnsemblBacteria" id="CAI48423">
        <property type="protein sequence ID" value="CAI48423"/>
        <property type="gene ID" value="NP_0664A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700921"/>
    <dbReference type="KEGG" id="nph:NP_0664A"/>
    <dbReference type="eggNOG" id="arCOG00777">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089876_7_1_2"/>
    <dbReference type="OrthoDB" id="202321at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03443">
        <property type="entry name" value="PaaI_thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039298">
        <property type="entry name" value="ACOT13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003736">
        <property type="entry name" value="PAAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00369">
        <property type="entry name" value="unchar_dom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21660:SF1">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21660">
        <property type="entry name" value="THIOESTERASE SUPERFAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioesterase" evidence="3">
        <location>
            <begin position="63"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="152" mass="16115" checksum="585691865C0AA3CA" modified="2017-05-10" version="1">MADAEPSDIPDEAAALLQEYLEAEHEFLSWLGFTVDALETGRMVATIPFDEKLTNPTDPPTIQGGVASTLVDVVGGIVLRPYLTDPINDDLSTINLNVNYLRPAAGDLTATAEVVRAGGSVGVSTIEVVSETPDGEEKPVVVGQGAYRLFRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU13</accession>
    <name>A0A1U7EU13_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Probable periplasmic serine protease</fullName>
            <ecNumber evidence="5">3.4.21.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">degP</name>
        <name evidence="5" type="ordered locus">NP_0660A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.4.21.-" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322057.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU13"/>
    <dbReference type="SMR" id="A0A1U7EU13"/>
    <dbReference type="STRING" id="348780.NP_0660A"/>
    <dbReference type="EnsemblBacteria" id="CAI48421">
        <property type="protein sequence ID" value="CAI48421"/>
        <property type="gene ID" value="NP_0660A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702661"/>
    <dbReference type="KEGG" id="nph:NP_0660A"/>
    <dbReference type="eggNOG" id="arCOG02833">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020120_2_2_2"/>
    <dbReference type="OrthoDB" id="350578at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051201">
        <property type="entry name" value="Chloro_Bact_Ser_Proteases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001940">
        <property type="entry name" value="Peptidase_S1C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43343">
        <property type="entry name" value="PEPTIDASE S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43343:SF3">
        <property type="entry name" value="PROTEASE DO-LIKE 8, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13180">
        <property type="entry name" value="PDZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13365">
        <property type="entry name" value="Trypsin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00834">
        <property type="entry name" value="PROTEASES2C"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 5" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="4">
        <location>
            <begin position="238"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI48421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="350" mass="35971" checksum="24C4DFD15CBE2DEF" modified="2017-05-10" version="1">MPELTRRRLLAAAAGGIAGLAGCAAGSRESPPPATPADPNPESQSRYTDVYEATIESVAFVGDEQGGGGSGFVYDGFVVTNQHVIADLEDVNVRFRRGEWRDAGVIATDVYADLAVLSTDIPDYAMSLPLVETVPPVGTEVLALGSPFGLESSVSAGIISGKNRSPNHPVTDFSIPNTVQTDAGLEPGNSGGPVVTMDGDVTGIAVAGAGANVGFAISPLLARRVLTELIQTGNYEHPYLGIQLIEVTPTVAEANDLDEARGVLVVETAPDGPPDQTLRGADGETVVGDQLVPTGGDVIVGLAGSQVDSNADLGTILALELSPGDRTTATVIRDGERQTIDVPIGSRPEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU45</accession>
    <name>A0A1U7EU45_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cobyrinate a,c-diamide synthase</fullName>
            <ecNumber evidence="8">6.3.5.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Cobyrinic acid a,c-diamide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 11" type="primary">cbiA</name>
        <name evidence="11" type="synonym">cobB</name>
        <name evidence="11" type="ordered locus">NP_0738A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the ATP-dependent amidation of the two carboxylate groups at positions a and c of cobyrinate, using either L-glutamine or ammonia as the nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>cob(II)yrinate + 2 L-glutamine + 2 ATP + 2 H2O = cob(II)yrinate a,c diamide + 2 L-glutamate + 2 ADP + 2 phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:26289"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58537"/>
            <dbReference type="ChEBI" id="CHEBI:58894"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 10/10.</text>
    </comment>
    <comment type="domain">
        <text evidence="8">Comprises of two domains. The C-terminal domain contains the binding site for glutamine and catalyzes the hydrolysis of this substrate to glutamate and ammonia. The N-terminal domain is anticipated to bind ATP and cobyrinate and catalyzes the ultimate synthesis of the diamide product. The ammonia produced via the glutaminase domain is probably translocated to the adjacent domain via a molecular tunnel, where it reacts with an activated intermediate.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">The a and c carboxylates of cobyrinate are activated for nucleophilic attack via formation of a phosphorylated intermediate by ATP. CbiA catalyzes first the amidation of the c-carboxylate, and then that of the a-carboxylate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the CobB/CbiA family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.11" evidence="8"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322096.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU45"/>
    <dbReference type="STRING" id="348780.NP_0738A"/>
    <dbReference type="EnsemblBacteria" id="CAI48460">
        <property type="protein sequence ID" value="CAI48460"/>
        <property type="gene ID" value="NP_0738A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702566"/>
    <dbReference type="KEGG" id="nph:NP_0738A"/>
    <dbReference type="eggNOG" id="arCOG00106">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022752_2_0_2"/>
    <dbReference type="OrthoDB" id="8896at2157"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00231"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042242">
        <property type="term" value="F:cobyrinic acid a,c-diamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03130">
        <property type="entry name" value="GATase1_CobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00027">
        <property type="entry name" value="CobB_CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004484">
        <property type="entry name" value="CbiA/CobB_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011698">
        <property type="entry name" value="GATase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00379">
        <property type="entry name" value="cobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002204">
        <property type="entry name" value="PRK01077.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010471">
        <property type="entry name" value="PRK13896.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43873">
        <property type="entry name" value="COBYRINATE A,C-DIAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43873:SF1">
        <property type="entry name" value="COBYRINATE A,C-DIAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07685">
        <property type="entry name" value="GATase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51274">
        <property type="entry name" value="GATASE_COBBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3 8 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobQ/CobB/MinD/ParA nucleotide binding" evidence="9">
        <location>
            <begin position="4"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="CobB/CobQ-like glutamine amidotransferase" evidence="10">
        <location>
            <begin position="233"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="313"/>
        </location>
    </feature>
    <feature type="site" description="Increases nucleophilicity of active site Cys" evidence="8">
        <location>
            <position position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI48460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="431" mass="45722" checksum="036976605C9B5546" modified="2017-05-10" version="1">MRGVVLAGTSSGVGKTVATLAVLQGLKEAGYEPQPAKAGPDFIDPSHHTAVCSRPSRSLDPWLSGEEGCRRNYWRGTGDVCVVEGMMGLYDGTNSTAAVAETLSLPVVLVVDAKAGMESVAATALGFSAYADRAGRDIEVAGVLAQRAHGGRHEDGIREALPDELAYLGRVPPLSGLEIPDRHLGLQMGTEAPIADETLADAAAHIDIERLVDIAAEPPRPEPSPERPATDVTVAVADDSAFCFRYPATIERLRERAAVTTFAPVAGDPVPDCDGVYLPGGYPELHAEALADGDTLDELGRRAADGLPVFGECGGLMALSETLTDTDDRTYAMAGVLPADVRMCERYQALDHVELRATDESAIASTDEHLRGHEFHYSTADVAADGTFAFELERGDGIADGREGLVEHRTLGTYAHFHADSGVFDRFVESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU72</accession>
    <name>A0A1U7EU72_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nicotinamide mononucleotide deamidase</fullName>
            <ecNumber evidence="2">3.5.1.42</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0822A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.1.42" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322138.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU72"/>
    <dbReference type="STRING" id="348780.NP_0822A"/>
    <dbReference type="EnsemblBacteria" id="CAI48502">
        <property type="protein sequence ID" value="CAI48502"/>
        <property type="gene ID" value="NP_0822A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702608"/>
    <dbReference type="KEGG" id="nph:NP_0822A"/>
    <dbReference type="eggNOG" id="arCOG04589">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030805_1_1_2"/>
    <dbReference type="OrthoDB" id="305448at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019159">
        <property type="term" value="F:nicotinamide-nucleotide amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.20">
        <property type="entry name" value="CinA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036653">
        <property type="entry name" value="CinA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008136">
        <property type="entry name" value="CinA_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00199">
        <property type="entry name" value="PncC_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02464">
        <property type="entry name" value="CinA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142433">
        <property type="entry name" value="CinA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CinA C-terminal" evidence="1">
        <location>
            <begin position="3"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="166" mass="17585" checksum="56CB64D564E032BF" modified="2017-05-10" version="1">MNIERAVGEMLADRGEYVATAESCTGGLIGSLLTDIPGASDYFDRSIVTYSYDAKLEDLGVPREILDAEGAVSEPVAEQMARGVRDHAGVTWGVATTGIAGPEGGSEGKPVGTVYIGVARAADWGTGDSWWSVERYVFDGSRTEIKRQIAVQALEDLKTAAASEPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUB0</accession>
    <name>A0A1U7EUB0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0914A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322184.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUB0"/>
    <dbReference type="EnsemblBacteria" id="CAI48548">
        <property type="protein sequence ID" value="CAI48548"/>
        <property type="gene ID" value="NP_0914A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700998"/>
    <dbReference type="KEGG" id="nph:NP_0914A"/>
    <dbReference type="eggNOG" id="arCOG10322">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1269905_0_0_2"/>
    <dbReference type="OrthoDB" id="350085at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="222" mass="25020" checksum="0BACA158826C8257" modified="2017-05-10" version="1">MAEEFERPPQGEFEREIRSFPEFFDRLRAEGALDIWDAVTSETEIEGLVYHHRGLKVPAHEGRFVWEPADETGRDVDAFSVDFGTVGPRSVWAVFDASREWDMYLVLFEEGAVVAWMSDAEFEAEESHRFPSKAAAVKSGQFSFGVLFRFGPDWVEREEWGLESTAPALLQQGDGQLLTPETESEFYRQTHAIPDEFRSEVETGAPPFCGLLEADVSADGDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUH5</accession>
    <name>A0A1U7EUH5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC-type transport system permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">abc25p</name>
        <name evidence="2" type="ordered locus">NP_1080A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322267.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUH5"/>
    <dbReference type="STRING" id="348780.NP_1080A"/>
    <dbReference type="EnsemblBacteria" id="CAI48631">
        <property type="protein sequence ID" value="CAI48631"/>
        <property type="gene ID" value="NP_1080A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702444"/>
    <dbReference type="KEGG" id="nph:NP_1080A"/>
    <dbReference type="eggNOG" id="arCOG02438">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071765_0_1_2"/>
    <dbReference type="OrthoDB" id="86287at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43471">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43471:SF1">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE PROTEIN NOSY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12679">
        <property type="entry name" value="ABC2_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="263"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="289" mass="30944" checksum="0E1F1F2465AB5E90" modified="2017-05-10" version="1">MSWMAVAEKDFRDAIRSRLLVVLTVLFAVFAAGAAYVVSEIDPPQQAALTGEVTTGLLIAGLVSATAVFIPIVAIAASYRSLAGERDSGSLKLLLSLPNSRRDIVAGKFVGRSAVVGVALLVGFAVGLVVTAVLADAFSVLEYLVFVAVSLLFALVFVAITVGVSAFTASTSRAAYGAFGLFVVFQFLWGVLVQGLAYALNGFSFPEGGEQFPEWIQNLANFLFIVDPTTAYQQGTLWVVRLLDDSQEAQETMAELPFYAQDWFGFVIMVVWIAVPLAVGYLRFDAADL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUH7</accession>
    <name>A0A1U7EUH7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC-type transport system ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">abc25a</name>
        <name evidence="6" type="ordered locus">NP_1082A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322268.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUH7"/>
    <dbReference type="STRING" id="348780.NP_1082A"/>
    <dbReference type="EnsemblBacteria" id="CAI48632">
        <property type="protein sequence ID" value="CAI48632"/>
        <property type="gene ID" value="NP_1082A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702443"/>
    <dbReference type="KEGG" id="nph:NP_1082A"/>
    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OrthoDB" id="87732at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03230">
        <property type="entry name" value="ABC_DR_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43335">
        <property type="entry name" value="ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43335:SF4">
        <property type="entry name" value="ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="4"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI48632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="302" mass="31669" checksum="B252F9A0E54561A7" modified="2017-05-10" version="1">MAAIELDGVEKAYGDVQALSGVDLEVEEGEIFGFLGPNGAGKSTTINILLDFVRPDAGSVRVLGYDAGEESVAVRERTGVLPEGFSVYDRLSAREHVAFAVDSKDADDDPEALLERVGLEDDAGRKAGGFSTGMRQRLALAMALAGDPDLLVLDEPSSGLDPNGARELREIVEDEAENGATVFFSSHILGQVEAVCDRVGIIRNGEVVATDSISGLRDAVGDGSTLVVEVDTVPDGTEDRLSSLDGVGNIAIDGTTLRVNCEDGVKTAVVSELEAAGATVEDFSTEEASLEELFAAYTEGQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUL4</accession>
    <name>A0A1U7EUL4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Probable oxidoreductase (Short-chain dehydrogenase family)</fullName>
            <ecNumber evidence="5">1.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_1160A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322307.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUL4"/>
    <dbReference type="STRING" id="348780.NP_1160A"/>
    <dbReference type="EnsemblBacteria" id="CAI48671">
        <property type="protein sequence ID" value="CAI48671"/>
        <property type="gene ID" value="NP_1160A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701071"/>
    <dbReference type="KEGG" id="nph:NP_1160A"/>
    <dbReference type="eggNOG" id="arCOG01259">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_9_2"/>
    <dbReference type="OrthoDB" id="10157at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05374">
        <property type="entry name" value="17beta-HSD-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051911">
        <property type="entry name" value="SDR_oxidoreductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43976">
        <property type="entry name" value="SHORT CHAIN DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43976:SF16">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE_REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketoreductase" evidence="4">
        <location>
            <begin position="4"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI48671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="275" mass="29951" checksum="511CDD04F25853FE" modified="2017-05-10" version="1">MSTRTVLITGCSSGIGRAAAYAFLENEWRVYATARNPADIQTLGEAGCDIGTINVRSTEDVERVVDRVIDEAGRIDALVNNAGYGQHGPIEDINDELFEKQFDVNVFGPHRLVRAVLPHMRERRDGTIINVSSVAGRLAAPGMGVYSASKHAIEGYSDSLRRELEPFDIDVSVVQPGPVETSFRDRVDDELGRLDRTDAYEDLYAFQEDASLFGGDSPVASHPGEVADVILEAASSTDPQPRYVVGTAAQLLVYASYLPDPIADSVFDAIRRFVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUM8</accession>
    <name>A0A1U7EUM8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NfeD domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_1188A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322321.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUM8"/>
    <dbReference type="STRING" id="348780.NP_1188A"/>
    <dbReference type="EnsemblBacteria" id="CAI48685">
        <property type="protein sequence ID" value="CAI48685"/>
        <property type="gene ID" value="NP_1188A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701079"/>
    <dbReference type="KEGG" id="nph:NP_1188A"/>
    <dbReference type="eggNOG" id="arCOG01912">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105301_0_0_2"/>
    <dbReference type="OrthoDB" id="157604at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052165">
        <property type="entry name" value="Membrane_assoc_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33507">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YBBJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33507:SF3">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YBBJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="215" mass="22093" checksum="1B3B1CB4FB204579" modified="2017-05-10" version="1">MAELLGYSVPFLLVLVGAVLMVMEGFAPGAHFIVVGVALLTAGLVGLLVGGVLPGAALPLVLAAVVLVAGGAALYGYRQFDFYGGKGSGQTSDSSSLQGQTGRVTQRVTEDDGEIKLDSGGFNPYYQARALDGEIGEGEKVMVIDPGGGNVVTVEAVSAFEDDIDRELAADRKRKTSNDEPTPGNESAAASEPESDGGSNEQDHSDDTESESEPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUT0</accession>
    <name>A0A1U7EUT0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">NUDIX family hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">NP_1302A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48742.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939452.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUT0"/>
    <dbReference type="STRING" id="348780.NP_1302A"/>
    <dbReference type="EnsemblBacteria" id="CAI48742">
        <property type="protein sequence ID" value="CAI48742"/>
        <property type="gene ID" value="NP_1302A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701109"/>
    <dbReference type="KEGG" id="nph:NP_1302A"/>
    <dbReference type="eggNOG" id="arCOG01072">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040940_5_2_2"/>
    <dbReference type="OrthoDB" id="51434at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010945">
        <property type="term" value="F:coenzyme A diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03426">
        <property type="entry name" value="NUDIX_CoAse_Nudt7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045121">
        <property type="entry name" value="CoAse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12992:SF11">
        <property type="entry name" value="MITOCHONDRIAL COENZYME A DIPHOSPHATASE NUDT8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12992">
        <property type="entry name" value="NUDIX HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="7">
        <location>
            <begin position="18"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI48742.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="199" mass="22171" checksum="A43CEAC2EDADC558" modified="2017-05-10" version="1">MDWERVAAHEPTAAVDGDREAAVLVPVIDRPDGYHLLFTKRAEHLGEHPGQMSFPGGGREPVDSSLEATATREAHEEIGLKPSEADVRGRLDDIPTVSNYAVRPFIAAIPDREYVPDEEEVAEIAVLSLSELTASENYESERREHANYGSVRIHYFRVDGYTVWGATGRMLVQFLELATDWTPPETVDRVVDPDADYPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUT2</accession>
    <name>A0A1U7EUT2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA(Ile2) 2-agmatinylcytidine synthetase TiaS</fullName>
            <shortName evidence="6">tRNA(Ile2)-agm2C synthetase</shortName>
            <ecNumber evidence="6">6.3.4.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">tRNA(Ile2) agmatidine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 11" type="primary">tiaS</name>
        <name evidence="11" type="ordered locus">NP_1318A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">ATP-dependent agmatine transferase that catalyzes the formation of 2-agmatinylcytidine (agm2C) at the wobble position (C34) of tRNA(Ile2), converting the codon specificity from AUG to AUA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>cytidine(34) in tRNA(Ile2) + agmatine + ATP + H2O = 2-agmatinylcytidine(34) in tRNA(Ile2) + AMP + 2 phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:43608"/>
            <dbReference type="Rhea" id="RHEA-COMP:10625"/>
            <dbReference type="Rhea" id="RHEA-COMP:10626"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58145"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:83545"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TiaS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.22" evidence="6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322385.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUT2"/>
    <dbReference type="STRING" id="348780.NP_1318A"/>
    <dbReference type="EnsemblBacteria" id="CAI48750">
        <property type="protein sequence ID" value="CAI48750"/>
        <property type="gene ID" value="NP_1318A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701113"/>
    <dbReference type="KEGG" id="nph:NP_1318A"/>
    <dbReference type="eggNOG" id="arCOG01115">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_675459_0_0_2"/>
    <dbReference type="OrthoDB" id="39189at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002101">
        <property type="term" value="P:tRNA wobble cytosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04482">
        <property type="entry name" value="RPA2_OBF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.1010">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.600.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01892">
        <property type="entry name" value="tRNA_Ile2_agm2C_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053870">
        <property type="entry name" value="TiaS-like_TCKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013696">
        <property type="entry name" value="TiaS_FLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024913">
        <property type="entry name" value="tRNA_Ile2__agm2C_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055394">
        <property type="entry name" value="Zn_ribbon_TiaS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40705">
        <property type="entry name" value="TRNA(ILE2) 2-AGMATINYLCYTIDINE SYNTHETASE TIAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40705:SF1">
        <property type="entry name" value="TRNA(ILE2) 2-AGMATINYLCYTIDINE SYNTHETASE TIAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08489">
        <property type="entry name" value="TiaS_FLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22641">
        <property type="entry name" value="TiaS_TCKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23783">
        <property type="entry name" value="Zn_ribbon_TiaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TiaS-like TCKD" evidence="9">
        <location>
            <begin position="3"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="TiaS FLD" evidence="8">
        <location>
            <begin position="140"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="TiaS C-terminal zinc ribbon" evidence="10">
        <location>
            <begin position="354"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="90"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF22641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI48750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="425" mass="45820" checksum="C1185A46B5165B42" modified="2017-05-10" version="1">MTVIGIDDTDSRERGMCTTYVADTIARALADAGHEVRRVLLVRCNPAVEHKTRGNAALAVHSDAPVEAASETARDIVESAAETADDRTNPGVVVAEGPPEAVPASVQSFSRTAIRGHHERHDAHAVIDSVGYQAHGWKNGRGVIGALAAVGAWKTLDDWTYEHIVYREPSRWGSDRDVSVESVFDAADSYYPEVWDTVDRGTGEAVCVPKTPGPVLYGIRGDDPEACRAVAANIDSEPADSERTFVTNQGTDGHLQDGAVGAARDGYAYRLSGTVAEAPETRRGGHVFFELVGDGDGTATLQCAAFEPTKRFRDRVRKLRVGDRITACGECTDGTLKLEKFAVRGLVRTERVTPVCPDCERTMESAGAGQGYRCRDCGTAAEGKAEQPLSRDLEIGWYEVPPRARRHIAKPLVRGGFDAPVHPES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUV3</accession>
    <name>A0A1U7EUV3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PQQ repeat protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1366A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322409.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUV3"/>
    <dbReference type="STRING" id="348780.NP_1366A"/>
    <dbReference type="EnsemblBacteria" id="CAI48774">
        <property type="protein sequence ID" value="CAI48774"/>
        <property type="gene ID" value="NP_1366A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701128"/>
    <dbReference type="KEGG" id="nph:NP_1366A"/>
    <dbReference type="eggNOG" id="arCOG02556">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027480_4_2_2"/>
    <dbReference type="OrthoDB" id="136681at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002372">
        <property type="entry name" value="PQQ_rpt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34512">
        <property type="entry name" value="CELL SURFACE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34512:SF30">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN ASSEMBLY FACTOR BAMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13360">
        <property type="entry name" value="PQQ_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyrrolo-quinoline quinone repeat" evidence="1">
        <location>
            <begin position="79"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrrolo-quinoline quinone repeat" evidence="1">
        <location>
            <begin position="185"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="389" mass="40428" checksum="7CC27E7D1DD99A3D" modified="2017-05-10" version="1">MNKRPASGTGHSVLRRSTRRQLLGAAAGATAALCGCLDDNGTDRTEPLDVSIGDVATYRYGPQRRGVADTTVPETAPDERWRLNTDWPVRAAPAVVGGVVYHCGNGLAAVDAVDGERLWRVDTDGPSETPPAVADGVVFAGTHRGRLHAINSDGTEHWTASGNGRIDAAPVVVDGRVYTASRATDGEVYAFEAGGREWTHEAVYRVNSPPAVADGLVFVGGWEGAVYGIDADSGERQWRTATDGPVLSSPAVADGRVFAGGNDGVVRALDVADGDERWQVETAATPTAPAVADGSLYVGDADGRLRRLDAATGEEGWQVTVASGIGVPPTVVEGTVVVGTTDGGVVAVDAADGTERWRVDTGERIRSQPVLAAGRVFVGSGRRLLALGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUV6</accession>
    <name>A0A1U7EUV6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SCP2 domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1376A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322414.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUV6"/>
    <dbReference type="STRING" id="348780.NP_1376A"/>
    <dbReference type="EnsemblBacteria" id="CAI48779">
        <property type="protein sequence ID" value="CAI48779"/>
        <property type="gene ID" value="NP_1376A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701131"/>
    <dbReference type="KEGG" id="nph:NP_1376A"/>
    <dbReference type="eggNOG" id="arCOG01842">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128031_0_0_2"/>
    <dbReference type="OrthoDB" id="51304at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1050.10">
        <property type="entry name" value="SCP2 sterol-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003033">
        <property type="entry name" value="SCP2_sterol-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036527">
        <property type="entry name" value="SCP2_sterol-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02036">
        <property type="entry name" value="SCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55718">
        <property type="entry name" value="SCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP2" evidence="1">
        <location>
            <begin position="57"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="143" mass="16144" checksum="3888864A0B26A22B" modified="2017-05-10" version="1">MSIAFPSEGDEWIEMYGELLDDNDDYTEAGSGWGVGFNGDFVFIIEPDDAYDGDPLYFFLGLEDGSCTDAYQVADPDDEEYGFIFRGPYSNWKRLFQGELGPVDGMMSGEFDIEGDMQKILQYSQAAVEMTETGRDIDTDFEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUX5</accession>
    <name>A0A1U7EUX5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DHH/RecJ family phosphoesterase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1428A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322440.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUX5"/>
    <dbReference type="STRING" id="348780.NP_1428A"/>
    <dbReference type="EnsemblBacteria" id="CAI48805">
        <property type="protein sequence ID" value="CAI48805"/>
        <property type="gene ID" value="NP_1428A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701147"/>
    <dbReference type="KEGG" id="nph:NP_1428A"/>
    <dbReference type="eggNOG" id="arCOG00423">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055955_0_0_2"/>
    <dbReference type="OrthoDB" id="18016at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052968">
        <property type="entry name" value="Nucleotide_metab_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42146">
        <property type="entry name" value="3',5'-CYCLIC-NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42146:SF1">
        <property type="entry name" value="OLIGORIBONUCLEASE NRNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="DHH phosphoesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="359" mass="39600" checksum="464365485E8D30BD" modified="2017-05-10" version="1">MDDWLIDDETIPLHRRSILPGEGFFVPDSVDETETEQEARETLAGADRVVVADPDADGLGCVALLRVAFDDARLLPAGPHELADALAYIDEYGEPGADVFICDLCPDSPSDIERLQSVAEDAAAVRWFDHHQWDDDLTERVREHAELVVGDSDTVCTTDVTLEAVAYDFPEQYAELAAVTRDHDLWLQEDPRSDDLADYAHWVEPETYVETVIEHGPDLPEEAVELLEERRVEKEALIEKAVERATFETVGEWTVGVTYGRCSQNEVAEAMREAGADASVIVKPAGSASIRGTEAFQRCHEVAQQVNGGGHPKAAGCKPDIYDDMLDYAHHWTTRGAVTKQVILDAFRSLADEPAEADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUZ5</accession>
    <name>A0A1U7EUZ5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type transport system permease protein (Probable substrate branched-chain amino acids)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">livM3</name>
        <name evidence="7" type="synonym">abc07p2</name>
        <name evidence="7" type="ordered locus">NP_1468A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322460.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUZ5"/>
    <dbReference type="STRING" id="348780.NP_1468A"/>
    <dbReference type="EnsemblBacteria" id="CAI48825">
        <property type="protein sequence ID" value="CAI48825"/>
        <property type="gene ID" value="NP_1468A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702385"/>
    <dbReference type="KEGG" id="nph:NP_1468A"/>
    <dbReference type="eggNOG" id="arCOG01274">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031365_2_0_2"/>
    <dbReference type="OrthoDB" id="30958at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06581">
        <property type="entry name" value="TM_PBP1_LivM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043428">
        <property type="entry name" value="LivM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30482:SF10">
        <property type="entry name" value="HIGH-AFFINITY BRANCHED-CHAIN AMINO ACID TRANSPORT PROTEIN BRAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30482">
        <property type="entry name" value="HIGH-AFFINITY BRANCHED-CHAIN AMINO ACID TRANSPORT SYSTEM PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="188"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="277"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="314"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="347" mass="37164" checksum="6FC37225D7AA4D0F" modified="2017-05-10" version="1">MSSIVDRVNETLGPRYADLSVPQFALVVVSLALLVVVAPALTAVGLMSGTLLRTLALANIFAIFAMAWDIQSGYTGYISFGHAVLSGAAAYSAALLITHVVPDINPWLLFPVAVLMALVVGLILAATTIRLSGPYFSLITLVAGLLFYRSTRTFSQWTQGEQGLLIGRLPVLPPFDGIFSAFDAEWRFIITVVPMLLIAAALLVISRSNVGTVLVAIRENEAAVESAGIDTNKFKVWSFVLSSIPMGIGGFLLAFFDGSVNPTDIVLLDRSVEFIAMAVIGGMGSILGPLAGAFLFVGLRDAILPAFLGGTERWLALWVIILVVIVLARDGLFRKLWHRLDDEGDAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV19</accession>
    <name>A0A1U7EV19_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Secreted glycoprotein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1480A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322466.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV19"/>
    <dbReference type="STRING" id="348780.NP_1480A"/>
    <dbReference type="EnsemblBacteria" id="CAI48831">
        <property type="protein sequence ID" value="CAI48831"/>
        <property type="gene ID" value="NP_1480A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701159"/>
    <dbReference type="KEGG" id="nph:NP_1480A"/>
    <dbReference type="HOGENOM" id="CLU_1976597_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="13362" checksum="D251D6FD1B8D77A4" modified="2017-05-10" version="1">MRRRQVLAAVPVALAGCVAAPEPGPPTGSPPNVFLDVDRHTEGFELRFAYGSPVTDDNTDRLYVTDDAARTETPWVGGDGAPASFPLEPGAELSVDADPSTEHRLVWVALDGDRSVSLAQFGGEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVA6</accession>
    <name>A0A1U7EVA6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">S8 family serine protease</fullName>
            <ecNumber evidence="9">3.4.21.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">NP_1682A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="9"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322565.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVA6"/>
    <dbReference type="STRING" id="348780.NP_1682A"/>
    <dbReference type="EnsemblBacteria" id="CAI48932">
        <property type="protein sequence ID" value="CAI48932"/>
        <property type="gene ID" value="NP_1682A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701223"/>
    <dbReference type="KEGG" id="nph:NP_1682A"/>
    <dbReference type="eggNOG" id="arCOG00702">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02510">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_490604_0_0_2"/>
    <dbReference type="OrthoDB" id="27270at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00146">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022409">
        <property type="entry name" value="PKD/Chitinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000601">
        <property type="entry name" value="PKD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035986">
        <property type="entry name" value="PKD_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF11">
        <property type="entry name" value="CEREVISIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18911">
        <property type="entry name" value="PKD_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00089">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49299">
        <property type="entry name" value="PKD domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5 6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="PKD/Chitinase" evidence="8">
        <location>
            <begin position="363"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="PKD/Chitinase" evidence="8">
        <location>
            <begin position="448"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="31"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI48932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="555" mass="56957" checksum="86BDD3BF58037449" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVC8</accession>
    <name>A0A1U7EVC8_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 15 16">Pyruvate kinase</fullName>
            <ecNumber evidence="5 15 16">2.7.1.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">pykA</name>
        <name evidence="20" type="ordered locus">NP_1746A</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>pyruvate + ATP = phosphoenolpyruvate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18157"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.40"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 16">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the pyruvate kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the PEP-utilizing enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.40" evidence="5 15 16"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322597.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVC8"/>
    <dbReference type="STRING" id="348780.NP_1746A"/>
    <dbReference type="EnsemblBacteria" id="CAI48964">
        <property type="protein sequence ID" value="CAI48964"/>
        <property type="gene ID" value="NP_1746A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703080"/>
    <dbReference type="KEGG" id="nph:NP_1746A"/>
    <dbReference type="eggNOG" id="arCOG01112">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG04120">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015439_0_2_2"/>
    <dbReference type="OrthoDB" id="56298at2157"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00188"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030955">
        <property type="term" value="F:potassium ion binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004743">
        <property type="term" value="F:pyruvate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphohistidine domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pyruvate kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008279">
        <property type="entry name" value="PEP-util_enz_mobile_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036637">
        <property type="entry name" value="Phosphohistidine_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001697">
        <property type="entry name" value="Pyr_Knase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015793">
        <property type="entry name" value="Pyrv_Knase_brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015806">
        <property type="entry name" value="Pyrv_Knase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004491">
        <property type="entry name" value="PRK05826.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004978">
        <property type="entry name" value="PRK06354.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01064">
        <property type="entry name" value="pyruv_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11817">
        <property type="entry name" value="PYRUVATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00391">
        <property type="entry name" value="PEP-utilizers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00224">
        <property type="entry name" value="PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01050">
        <property type="entry name" value="PYRUVTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52009">
        <property type="entry name" value="Phosphohistidine domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="PK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13 16" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="9 16 20" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0630">Potassium</keyword>
    <keyword evidence="14 20" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16 20" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate kinase barrel" evidence="17">
        <location>
            <begin position="2"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate kinase C-terminal" evidence="19">
        <location>
            <begin position="353"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilising enzyme mobile" evidence="18">
        <location>
            <begin position="520"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="NCBIfam" id="TIGR01064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAI48964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="593" mass="61703" checksum="CD20AE20A8C2BEE2" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVF2</accession>
    <name>A0A1U7EVF2_NATPD</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1836A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322641.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVF2"/>
    <dbReference type="EnsemblBacteria" id="CAI49009">
        <property type="protein sequence ID" value="CAI49009"/>
        <property type="gene ID" value="NP_1836A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701270"/>
    <dbReference type="KEGG" id="nph:NP_1836A"/>
    <dbReference type="eggNOG" id="arCOG04710">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133053_0_0_2"/>
    <dbReference type="OrthoDB" id="330871at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="170" mass="17571" checksum="F73A150C1417302D" modified="2017-05-10" version="1">MLRTVRDAGPTVLVPLAWLVVAAAHRGLVSDHALLVAHVVMAAFIAGFAVTGWSEMDDGALRAWRAVLVVGLGVTLSGIAGFLLPSEPLRAVSIVGWMLLPAAGLLYTGYLFTAARTVYFGAGWLSVAGAVVYTISLCGDGPLVLVGIALVGAGQTVGIVDAAMRDDREQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVG8</accession>
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        <submittedName>
            <fullName evidence="4">Receiver box response regulator</fullName>
        </submittedName>
    </protein>
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        <name evidence="4" type="ordered locus">NP_1846A</name>
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    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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            <authorList>
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                <person name="Pfeiffer F."/>
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                <person name="Rodewald K."/>
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            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49014.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="NP_1846A"/>
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    <dbReference type="KEGG" id="nph:NP_1846A"/>
    <dbReference type="eggNOG" id="arCOG06363">
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43102:SF2">
        <property type="entry name" value="GAF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43102">
        <property type="entry name" value="SLR1143 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GAF" evidence="3">
        <location>
            <begin position="150"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49014.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="301" mass="32868" checksum="E80642C0777925D2" modified="2017-05-10" version="1">MAQTVLCVDTPDRVSTVTDAVAAGDDLNAVSATSVSDATEVLIDDPVVCVVTAYDLSDGTGLDIVGAIREHAPQTPCVLFTDASPGEIDTAAFEETIVEYLNRDLPDADERLSFVVDDVISHSAQVGFLAPKNEGERLETLAAYDVDALPIEDSFDRLTDLIADRFDVAVSFIGLIEREEENFLACTGADWDSLTREDTICTHSMLQEDVMVVEDIDEDKRFSENEQLHDLGIVSYAGANMTAPDGSVIGQVCVLDHEPRSYDAGERETLQQYAETAMEILELRRRLHELDSESAATEVDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVL4</accession>
    <name>A0A1U7EVL4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ISH4-type transposase ISNph2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_1938A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322692.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVL4"/>
    <dbReference type="STRING" id="348780.NP_1938A"/>
    <dbReference type="EnsemblBacteria" id="CAI49060">
        <property type="protein sequence ID" value="CAI49060"/>
        <property type="gene ID" value="NP_1938A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701312"/>
    <dbReference type="KEGG" id="nph:NP_1938A"/>
    <dbReference type="eggNOG" id="arCOG02132">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044348_1_2_2"/>
    <dbReference type="OrthoDB" id="86086at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024445">
        <property type="entry name" value="Tnp_ISXO2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051354">
        <property type="entry name" value="Transposase_27_IS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024442">
        <property type="entry name" value="Transposase_Zn_ribbon"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033547">
        <property type="entry name" value="transpos_IS1595"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33293">
        <property type="entry name" value="INSERTION ELEMENT IS1 1 PROTEIN INSB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33293:SF1">
        <property type="entry name" value="INSERTION ELEMENT IS1 1 PROTEIN INSB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12762">
        <property type="entry name" value="DDE_Tnp_IS1595"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12760">
        <property type="entry name" value="Zn_ribbon_IS1595"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01126">
        <property type="entry name" value="DDE_Tnp_IS1595"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ISXO2-like transposase" evidence="2">
        <location>
            <begin position="120"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="294" mass="33964" checksum="9900A690A71C531F" modified="2017-05-10" version="1">MFPFELLSSEASAANLLEQVRWREGLCCPRCRSESVIKHGSYREYQRYLCKDCDRTFNDKTGTIFAHAKIGLDKLLFAFYSLLRFNTSIRQLDAEIDVSYRSLRRRVEQFARTLDAPAINLVGPVEIDEFYVSAGKKGRERDRESRSRALSKRGRGTYEEDKPPVFTLVDRGTGQRYVVPAKSADEATVRLLLENHEKESLTVYTDGFRAYDPLDDDEDFHRESVIHGDGEYVDGDAHVNTCESHASLARRWLSPHRGVSKDKLTAYLRPFQLRRRILRKPGREALKQIIREVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVS3</accession>
    <name>A0A1U7EVS3_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Archaeal Type IV pilin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_2110A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVS3"/>
    <dbReference type="STRING" id="348780.NP_2110A"/>
    <dbReference type="EnsemblBacteria" id="CAI49146">
        <property type="protein sequence ID" value="CAI49146"/>
        <property type="gene ID" value="NP_2110A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_2110A"/>
    <dbReference type="eggNOG" id="arCOG07560">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_333073_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012859">
        <property type="entry name" value="Pilin_N_archaeal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07790">
        <property type="entry name" value="Pilin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="Archaeal Type IV pilin N-terminal" evidence="3">
        <location>
            <begin position="16"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="767"/>
            <end position="794"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="858" mass="93121" checksum="5CFBA26C995B3D3B" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVT7</accession>
    <name>A0A1U7EVT7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Flagella cluster protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2152A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322795.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVT7"/>
    <dbReference type="STRING" id="348780.NP_2152A"/>
    <dbReference type="EnsemblBacteria" id="CAI49167">
        <property type="protein sequence ID" value="CAI49167"/>
        <property type="gene ID" value="NP_2152A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701366"/>
    <dbReference type="KEGG" id="nph:NP_2152A"/>
    <dbReference type="eggNOG" id="arCOG02894">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2629569_0_0_2"/>
    <dbReference type="OrthoDB" id="191000at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055809">
        <property type="entry name" value="DUF7385"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24110">
        <property type="entry name" value="DUF7385"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="77" mass="8928" checksum="A1CBCA17C7A27E65" modified="2017-05-10" version="1">MTDNGFDIHANRHRLKQLKDSGDTKLFENRDDVECPACGEPFSRLFSTKQRATSFPKNDGARFCLVRDGEFVHLFRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVU1</accession>
    <name>A0A1U7EVU1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2164A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322801.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVU1"/>
    <dbReference type="STRING" id="348780.NP_2164A"/>
    <dbReference type="EnsemblBacteria" id="CAI49173">
        <property type="protein sequence ID" value="CAI49173"/>
        <property type="gene ID" value="NP_2164A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701368"/>
    <dbReference type="KEGG" id="nph:NP_2164A"/>
    <dbReference type="HOGENOM" id="CLU_2679047_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="74" mass="7658" checksum="9CCA5A0B6CE05CD8" modified="2017-05-10" version="1">MIRLFAAAGFVVGFALVLAAPIALLHAMADSRAVTDLPTLTQGDVYLIAGVMGLGLLLAGFSAIMWLASDIRPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVV2</accession>
    <name>A0A1U7EVV2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 15">tRNA-splicing ligase RtcB</fullName>
            <ecNumber evidence="15">6.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">rtcB1</name>
        <name evidence="15" type="synonym">rtcB</name>
        <name evidence="17" type="ordered locus">NP_2178A</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential for tRNA splicing and maturation. Acts by directly joining spliced tRNA halves to mature-sized tRNAs. Joins RNA with 2',3'-cyclic-phosphate or 3'-phosphate ends to RNA with 5'-hydroxy ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP + H2O = a ribonucleotidyl-ribonucleotide-RNA + GMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:68080"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="Rhea" id="RHEA-COMP:17355"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:173118"/>
            <dbReference type="EC" id="6.5.1.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 3'-end 3'-phospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP = a ribonucleotidyl-ribonucleotide-RNA + GMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:68076"/>
            <dbReference type="Rhea" id="RHEA-COMP:10463"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="Rhea" id="RHEA-COMP:17355"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:83062"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:173118"/>
            <dbReference type="EC" id="6.5.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14 15">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="14 15">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 15">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 15">Belongs to the RtcB family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.-" evidence="15"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322808.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVV2"/>
    <dbReference type="STRING" id="348780.NP_2178A"/>
    <dbReference type="EnsemblBacteria" id="CAI49180">
        <property type="protein sequence ID" value="CAI49180"/>
        <property type="gene ID" value="NP_2178A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703193"/>
    <dbReference type="KEGG" id="nph:NP_2178A"/>
    <dbReference type="eggNOG" id="arCOG04246">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022279_0_1_2"/>
    <dbReference type="OrthoDB" id="9887at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003972">
        <property type="term" value="F:RNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0170057">
        <property type="term" value="F:RNA ligase (GTP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1860.10:FF:000001">
        <property type="entry name" value="tRNA-splicing ligase RtcB homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1860.10">
        <property type="entry name" value="tRNA-splicing ligase RtcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001233">
        <property type="entry name" value="RtcB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036025">
        <property type="entry name" value="RtcB-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11118">
        <property type="entry name" value="RNA-SPLICING LIGASE RTCB HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11118:SF1">
        <property type="entry name" value="RNA-SPLICING LIGASE RTCB HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01139">
        <property type="entry name" value="RtcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103365">
        <property type="entry name" value="Hypothetical protein PH1602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 15 17" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 14 15" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="173"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="173"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="active site" description="GMP-histidine intermediate" evidence="12">
        <location>
            <position position="408"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="207"/>
            <end position="211"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="333"/>
            <end position="334"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="382"/>
            <end position="385"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="389"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="408"/>
            <end position="411"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="484"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU371113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAI49180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="485" mass="52229" checksum="1E63C6D9C246FBEF" modified="2017-05-10" version="1">MTTFEAGNITLHKVREHVWEIPPEDGMRVPARVFASERLLEEIADDKSLQQLKNTTHLPGIEKYAICMPDGHQGYGFPVGGVAAIDAENGCISPGAVGYDINCGVRMVKTNLTYDDVSGMEEELVEALFDAVPTGLGGGGIVQGDRDTVDAVLERGVDWAREEGYAVKSDLEHCEDEGVRPDADPSAVSQKAKDRGRNQLGSLGSGNHFLEVQRVTDVFDADIADAYGLEADQIVILIHCGSRGLGHQVCNDYLRDIEQAHSGLLSKLPDKELAAAPAGSQLAEDYYGAMCAAINFAWVNRQLITHQTRTVFADVFGEPWEDLGMELLYDVAHNIAKKEVHDVDGEDKELYVHRKGATRAFPAGRPEVPSAYRDVGQPVIIPGSMGAGSYVLCGGEQSLDISFGSTAHGAGRLMSRTQAKDEFWGGDVQDDLRGQEVYVKAESGATIAEEAPGVYKDVDEVVSVSDALGIGDKVVRVAPVCNIKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVZ7</accession>
    <name>A0A1U7EVZ7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NADH dehydrogenase-like complex subunit I</fullName>
            <ecNumber evidence="7">1.6.5.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">nuoI</name>
        <name evidence="7" type="ordered locus">NP_2300A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.6.5.-" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322868.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVZ7"/>
    <dbReference type="STRING" id="348780.NP_2300A"/>
    <dbReference type="EnsemblBacteria" id="CAI49241">
        <property type="protein sequence ID" value="CAI49241"/>
        <property type="gene ID" value="NP_2300A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702993"/>
    <dbReference type="KEGG" id="nph:NP_2300A"/>
    <dbReference type="eggNOG" id="arCOG01543">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067218_4_3_2"/>
    <dbReference type="OrthoDB" id="23478at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3270:FF:000010">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010226">
        <property type="entry name" value="NADH_quinone_OxRdtase_chainI"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01971">
        <property type="entry name" value="NuoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10849:SF35">
        <property type="entry name" value="FORMATE HYDROGENLYASE SUBUNIT 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10849">
        <property type="entry name" value="NADH DEHYDROGENASE UBIQUINONE IRON-SULFUR PROTEIN 8, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="38"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="73"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
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    </dbReference>
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        </location>
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            <end position="52"/>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="202" mass="23606" checksum="2462B06A7D15A883" modified="2017-05-10" version="1">MKGFTDEERDRIERELREAGRELFASFGLERTRISDITDEVGIGTSTFYQFYDSKAELYLDVIYEEVTQIGDDVADEIADAESLEEEIRRTLTLLFRKLETNDILYQVLVEDDGQRLRNQLDADVQKRHHENKYRTLGPLVERWTENERFRADDPEVALDMLGAVPQFVAHRRQFDALDRNDAYEPARELLVETLTDGLVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW72</accession>
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        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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                <person name="Pfeiffer F."/>
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            <dbReference type="PubMed" id="16169924"/>
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            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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    </dbReference>
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    <dbReference type="GeneID" id="3701451"/>
    <dbReference type="KEGG" id="nph:NP_2462A"/>
    <dbReference type="eggNOG" id="arCOG02174">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="BLR2997 PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="MEMBRANE PROTEIN MJ1562-RELATED"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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        </location>
    </feature>
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        </location>
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        <location>
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        <location>
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        <location>
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    </feature>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="835" mass="89589" checksum="FEA470C3D4299AB3" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW83</accession>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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                <person name="Rodewald K."/>
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            </authorList>
            <dbReference type="PubMed" id="16169924"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        <location>
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            <end position="121"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI49334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="121" mass="12872" checksum="63837A7499BBBE24" modified="2017-05-10" version="1">MFEQFSSGYYLGRLYVEPYGGDHAVMHRNHHEQVNEQLYDGKEAPLVMKIGSAHVPVHSADDVPGRTVGLPESALSAAGVENPPTLQEVLLAKADRAKQLLGLAPDSPARPDGAADGPAGF</sequence>
</entry>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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        </source>
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    <dbReference type="EnsemblBacteria" id="CAI49463">
        <property type="protein sequence ID" value="CAI49463"/>
        <property type="gene ID" value="NP_2744A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701542"/>
    <dbReference type="KEGG" id="nph:NP_2744A"/>
    <dbReference type="eggNOG" id="arCOG04124">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155659_4_2_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005651">
        <property type="entry name" value="Trm112-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038101">
        <property type="entry name" value="Trm112_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33505:SF4">
        <property type="entry name" value="PROTEIN PREY, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33505">
        <property type="entry name" value="ZGC:162634"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03966">
        <property type="entry name" value="Trm112p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158997">
        <property type="entry name" value="Trm112p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="64" mass="7204" checksum="67CC448172DB8C74" modified="2017-05-10" version="1">MKESLLDILCCPLDKGDLELEVIREDDDEILEGRLLCEECGEEYPIEDGIPNLLPPDMRDEAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWL7</accession>
    <name>A0A1U7EWL7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Probable oxidoreductase (Aldo-keto reductase family protein)</fullName>
            <ecNumber evidence="3">1.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2796A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323114.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWL7"/>
    <dbReference type="STRING" id="348780.NP_2796A"/>
    <dbReference type="EnsemblBacteria" id="CAI49489">
        <property type="protein sequence ID" value="CAI49489"/>
        <property type="gene ID" value="NP_2796A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702439"/>
    <dbReference type="KEGG" id="nph:NP_2796A"/>
    <dbReference type="eggNOG" id="arCOG01617">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_0_2"/>
    <dbReference type="OrthoDB" id="7236at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19079">
        <property type="entry name" value="AKR_EcYajO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000004">
        <property type="entry name" value="Oxidoreductase, aldo/keto reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050523">
        <property type="entry name" value="AKR_Detox_Biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43364:SF4">
        <property type="entry name" value="NAD(P)-LINKED OXIDOREDUCTASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43364">
        <property type="entry name" value="NADH-SPECIFIC METHYLGLYOXAL REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="2">
        <location>
            <begin position="15"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="324" mass="36752" checksum="1580FAA22CF35348" modified="2017-05-10" version="1">MEYTTLGATGIEVSRICLGCMSFGDDDWREWVRGEEFGHELVERALDLGINFFDTANMYSRGESERILGDALDGHREESVVATKVYFPMRDGDPNSGGLSRKTVEQELSASLDRLGMDTVDLYQIHRWDYDTPIAQTLRAMTDAVQRGQVRHIGASSMWTHQLAEALHTSDREGLERFATMQNHYNLAYREEEREMLPFCEKERLGVMPWSPMARGYLTRPHEEYMSTKRAETDDYAQEHPYADNGGREINERVQELAADKGVSMAQIALSWVLHKEWVDAPIVGASKLSHLEDAVEALEIDLSDSDLAYLEAPYEPVPVSGHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWN9</accession>
    <name>A0A1U7EWN9_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Small ribosomal subunit protein uS9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 8" type="primary">rps9</name>
        <name evidence="8" type="ordered locus">NP_2840A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the universal ribosomal protein uS9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323136.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWN9"/>
    <dbReference type="STRING" id="348780.NP_2840A"/>
    <dbReference type="EnsemblBacteria" id="CAI49511">
        <property type="protein sequence ID" value="CAI49511"/>
        <property type="gene ID" value="NP_2840A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703189"/>
    <dbReference type="KEGG" id="nph:NP_2840A"/>
    <dbReference type="eggNOG" id="arCOG04243">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046483_4_0_2"/>
    <dbReference type="OrthoDB" id="52677at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000051">
        <property type="entry name" value="30S ribosomal protein S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00532_A">
        <property type="entry name" value="Ribosomal_uS9_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_uS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019958">
        <property type="entry name" value="Ribosomal_uS9_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020574">
        <property type="entry name" value="Ribosomal_uS9_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001749">
        <property type="entry name" value="PRK00474.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03627">
        <property type="entry name" value="uS9_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569:SF16">
        <property type="entry name" value="RIBOSOMAL PROTEIN S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569">
        <property type="entry name" value="RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00360">
        <property type="entry name" value="RIBOSOMAL_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="132" mass="14446" checksum="1B7E1AB0747D4022" modified="2017-05-10" version="1">MVTNTSGKKKTAVARATVSDGSGRVRINAEPVELVEPEQARLKMLEPFRIAADDLRDEVDIEVTVSGGGFAGQADAVRTAIARGLVEHTNDAELRDAFMSFDRSLLVNDVRQSEPKKWGGPGARARYQKSYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWR9</accession>
    <name>A0A1U7EWR9_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA polymerase IV</fullName>
            <shortName evidence="2">Pol IV</shortName>
            <ecNumber evidence="2">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">polY</name>
        <name evidence="2" type="synonym">dbh</name>
        <name evidence="5" type="ordered locus">NP_2918A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the DNA polymerase type-Y family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323174.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWR9"/>
    <dbReference type="STRING" id="348780.NP_2918A"/>
    <dbReference type="EnsemblBacteria" id="CAI49550">
        <property type="protein sequence ID" value="CAI49550"/>
        <property type="gene ID" value="NP_2918A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701598"/>
    <dbReference type="KEGG" id="nph:NP_2918A"/>
    <dbReference type="eggNOG" id="arCOG04582">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012348_1_2_2"/>
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    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="CDD" id="cd03586">
        <property type="entry name" value="PolY_Pol_IV_kappa"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
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        <property type="entry name" value="DNA polymerase, Y-family, little finger domain"/>
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        <property type="entry name" value="DNA/RNA_pol_sf"/>
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        <property type="entry name" value="DNA_pol_Y-fam_little_finger"/>
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        <property type="entry name" value="DNA_polymerase-Y"/>
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        <property type="entry name" value="DNApol_IV"/>
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        <property type="entry name" value="PolY_HhH_motif"/>
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        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001126">
        <property type="entry name" value="UmuC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002677">
        <property type="entry name" value="PRK02406.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11076:SF33">
        <property type="entry name" value="DNA POLYMERASE KAPPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11076">
        <property type="entry name" value="DNA REPAIR POLYMERASE UMUC / TRANSFERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IMS"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="IMS_C"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="IMS_HHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100879">
        <property type="entry name" value="Lesion bypass DNA polymerase (Y-family), little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50173">
        <property type="entry name" value="UMUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="2" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 5" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0515">Mutator protein</keyword>
    <keyword evidence="2 5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="UmuC" evidence="4">
        <location>
            <begin position="14"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="248"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="371"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="248"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="394"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Substrate discrimination" evidence="2">
        <location>
            <position position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="404" mass="43765" checksum="29E5F6D13C97D114" modified="2017-05-10" version="1">MPQLPGVDPRRQVVCHVDVDCFYAACERLREPKLRDEPVVVGMGYEPGADHGAVATASYEAREHGVESAMAISEALKRLPQRTDSESGEAGTGYYRPVDMAYYESVSEDVQAVLDDTAETVRNVSIDEAYLDLGDLAWDDAEAFGRQLKADIEAAAGVVASVGIAPTMATAKLASDAEKPDGLVIVDPDSVATFLKPIPVAELHGVGPVTAAELRERGYETAGDVAAADETALVDAFGERGRDIYRQARGEDDRVVEPKGRPKSLSSESAFPKPTDDGDRKRRKLTALAADVTERATAKDALYRTVGIKVVEPPFDVHTRERSLSGPVDDPKLVEEIAVDLLAEFDDPAVRKLGVRVSNLSFDDREQATLGQWADGTADQEPVRLSHVRRSGETDTQLELSQFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWU5</accession>
    <name>A0A1U7EWU5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">P-type transport ATPase (Probable substrate copper/metal cation)</fullName>
            <ecNumber evidence="12">3.6.3.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">tpa04</name>
        <name evidence="12" type="ordered locus">NP_2972A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.-" evidence="12"/>
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        <property type="protein sequence ID" value="CAI49577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323201.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWU5"/>
    <dbReference type="STRING" id="348780.NP_2972A"/>
    <dbReference type="EnsemblBacteria" id="CAI49577">
        <property type="protein sequence ID" value="CAI49577"/>
        <property type="gene ID" value="NP_2972A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703351"/>
    <dbReference type="KEGG" id="nph:NP_2972A"/>
    <dbReference type="eggNOG" id="arCOG01576">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001771_0_3_2"/>
    <dbReference type="OrthoDB" id="8588at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043682">
        <property type="term" value="F:P-type divalent copper transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055070">
        <property type="term" value="P:copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520">
        <property type="entry name" value="ATP7, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520:SF8">
        <property type="entry name" value="P-TYPE CU(+) TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="164"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="240"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="264"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="412"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="439"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="771"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="11">
        <location>
            <begin position="81"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI49577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="818" mass="86092" checksum="0EFF8F5B8DA91647" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX16</accession>
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            <taxon>Halobacteriales</taxon>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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        <text evidence="1">Belongs to the SCO1/2 family.</text>
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    </dbReference>
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    </dbReference>
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    <dbReference type="EnsemblBacteria" id="CAI49659">
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        <property type="gene ID" value="NP_3136A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701664"/>
    <dbReference type="KEGG" id="nph:NP_3136A"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="27579at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR003782">
        <property type="entry name" value="SCO1/SenC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12151">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12151:SF25">
        <property type="entry name" value="LINALOOL DEHYDRATASE_ISOMERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02630">
        <property type="entry name" value="SCO1-SenC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0186">Copper</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="61"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
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        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
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        <location>
            <begin position="102"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010996"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR603782-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR603782-2"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI49659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="237" mass="25946" checksum="933AD9302AF5667F" modified="2017-05-10" version="1">MTQPDGRSSSALSRRQYLAAFGAAGAVSAAGCLGDVFGGGDAAVVLDEPSDELDSDHLPYPTHGESFPDFELPDPIAGETISTDSETLADQTLIVTGFYAFCPAECLLLIGSMAETQRVVVNDGYADDVTFLPITFDPERDTPEELDDYADEMNVDRDAGNWRFLRPEDEAEARAVVDEKLAINYEQDDGAGDTYEFIHNTLTYLVNPDRYVERVYRDDDLPVETLADEAMTVADAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX39</accession>
    <name>A0A1U7EX39_NATPD</name>
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        <submittedName>
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        </submittedName>
    </protein>
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    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <lineage>
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            <taxon>Methanobacteriati</taxon>
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            <taxon>Halobacteriales</taxon>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    <dbReference type="STRING" id="348780.NP_3150A"/>
    <dbReference type="EnsemblBacteria" id="CAI49666">
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        <property type="gene ID" value="NP_3150A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701670"/>
    <dbReference type="KEGG" id="nph:NP_3150A"/>
    <dbReference type="eggNOG" id="arCOG04573">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_709039_0_0_2"/>
    <dbReference type="OrthoDB" id="242713at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="141"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="406"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="254"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="340"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="144"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="417"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="429"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="453"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="468"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="478" mass="50085" checksum="C56949A47D514D03" modified="2017-05-10" version="1">MSNADPTEVVTASSDGVTVEKSFEPDDFPVPAIAFAIESERDESVSVRITDTVPEDVAPEDIGFHPDYGAEFWDVEEGQIVFERDFDAGEEFTTVYGLRGGDAEVARKFMSEPEIESVDPPLSGPERAAAQAAGDIEMGIGPIADDATASGAGRTTAGADDRQPSAGQADDGEQAAIAPGDEDSLVAQLATEINAGAVDRQTVLYLRDALGVNLASASVEARIEQLQSSVADLDAYTSALEAFLDENGDAQQLIQDVRDDYEETTARIDAVESVADEAVEATDDIDARLDDVEETFEEQLDDVETRLDDVVTEVEDDLEAIDEEVEVAVESELEALESRVDSIADDAAAATDGIEELREELSEVETGLQEETETRLDELEAEIESLEAELSDVAELRDRLATALSGLGGNVPAVGDGEPDTEAGEAENDTSPAVAADAADDVAGSGSGDGTDDAAQASDTDDGAAAGDDTEADTTDAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX54</accession>
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            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
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    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
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            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="STRING" id="348780.NP_3214A"/>
    <dbReference type="EnsemblBacteria" id="CAI49698">
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        <property type="gene ID" value="NP_3214A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701685"/>
    <dbReference type="KEGG" id="nph:NP_3214A"/>
    <dbReference type="eggNOG" id="arCOG04777">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165229_0_0_2"/>
    <dbReference type="OrthoDB" id="280959at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055537">
        <property type="entry name" value="DUF7113"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23425">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="113" mass="12313" checksum="1C0374BFD5DB4724" modified="2017-05-10" version="1">MLQVRGSAHGTALTGTLYEPDDEPPTFSGAPDDNAPYVWVCDAFYEVESGGRTQTLGDRTLQVAFESPMPRGFDDREAALDAAADHVRTQFARLGVDRSTVDIEVIEAEPQPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX68</accession>
    <name>A0A1U7EX68_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">JAB domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">NP_3222A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323324.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
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    <dbReference type="STRING" id="348780.NP_3222A"/>
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        <property type="protein sequence ID" value="CAI49702"/>
        <property type="gene ID" value="NP_3222A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701689"/>
    <dbReference type="KEGG" id="nph:NP_3222A"/>
    <dbReference type="eggNOG" id="arCOG01139">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116578_0_0_2"/>
    <dbReference type="OrthoDB" id="4612at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08072">
        <property type="entry name" value="MPN_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028090">
        <property type="entry name" value="JAB_dom_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14464">
        <property type="entry name" value="Prok-JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102712">
        <property type="entry name" value="JAB1/MPN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MPN" evidence="6">
        <location>
            <begin position="19"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="168" mass="18575" checksum="F5A4CBFECE988F71" modified="2017-05-10" version="1">MGLLGSLWKPTLFRSKEVIGIADDALQFALQASEDSHPNEYMGQLRGQDARSLGLDRDGTVITDVLLAPGTKTNPTSAEFKPTYMPNDLNSVGSVHSHPNGVLRPSDADLATFTKGQVHIIVGAPYRRSDWRAFDREGEPIDLDVLSVDVPDDQFFDFTQADIDREMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX79</accession>
    <name>A0A1U7EX79_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">NP_3356A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323389.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX79"/>
    <dbReference type="STRING" id="348780.NP_3356A"/>
    <dbReference type="EnsemblBacteria" id="CAI49769">
        <property type="protein sequence ID" value="CAI49769"/>
        <property type="gene ID" value="NP_3356A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701747"/>
    <dbReference type="KEGG" id="nph:NP_3356A"/>
    <dbReference type="eggNOG" id="arCOG02329">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_550560_0_0_2"/>
    <dbReference type="OrthoDB" id="8127at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004673">
        <property type="term" value="F:protein histidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050736">
        <property type="entry name" value="Sensor_HK_Regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43711:SF1">
        <property type="entry name" value="HISTIDINE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43711">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="8">
        <location>
            <begin position="137"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="185"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI49769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="495" mass="52829" checksum="F56D51BDF146D5EA" modified="2017-05-10" version="1">MNSIAEEPPVGPILAVAGLVVIGIAAWRTVVAETIGPMLIAALLPVLLTGGLLLVLGYRIHALSERTDRILLWTGVGAIAVTAAVGAILLRVHLLDVRLDPLLILLAVGSVGAAGAAGAAFIDTKRRDTLARYRQRVTELETRLADTSGVGLVSLTPAGEVDDWSAGAADILGYDAEGIRGEHLSTLYPDDNDDIRHPQLKPGQDTDDDTHEPSARHHLQQALRTDHVVLDGRLRRADDEWVPVTGTLTAAEDGETLVGYTLVVSARDHRGYTAPSHEREATPDDRSGHWGRQFERLAPIVDETLREPHATASEAVTNERTRNDSGRLDDAATALEEMADPLAEIHALAAAHRDADALERVALDSAVESAWEAADPMWARLETEELTAVNAVPERLERLLTELFENAVEHGGDVTVRVGMLPDGIYVADDGPGLEAPETVFEVGYTTDDGDGTGLGLPTVAAIAESHAWDVSAAESADGGARFEVRGMQTFGRAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXH1</accession>
    <name>A0A1U7EXH1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Probable DEAD/DEAH box helicase</fullName>
            <ecNumber evidence="6">3.6.4.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">NP_3562A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.4.-" evidence="6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323492.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXH1"/>
    <dbReference type="STRING" id="348780.NP_3562A"/>
    <dbReference type="EnsemblBacteria" id="CAI49872">
        <property type="protein sequence ID" value="CAI49872"/>
        <property type="gene ID" value="NP_3562A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701807"/>
    <dbReference type="KEGG" id="nph:NP_3562A"/>
    <dbReference type="eggNOG" id="arCOG00555">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000809_3_2_2"/>
    <dbReference type="OrthoDB" id="36796at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18797">
        <property type="entry name" value="SF2_C_Hrq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018973">
        <property type="entry name" value="MZB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47957">
        <property type="entry name" value="ATP-DEPENDENT HELICASE HRQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47957:SF3">
        <property type="entry name" value="ATP-DEPENDENT HELICASE HRQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09369">
        <property type="entry name" value="MZB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="4">
        <location>
            <begin position="68"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="5">
        <location>
            <begin position="302"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI49872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="817" mass="90079" checksum="2538BE67C2A53D0B" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXK8</accession>
    <name>A0A1U7EXK8_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Molybdate/tungstate import ATP-binding protein WtpC</fullName>
            <ecNumber evidence="15">7.3.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">abc24a</name>
        <name evidence="20" type="ordered locus">NP_3648A</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Part of the ABC transporter complex WtpABC involved in molybdate/tungstate import. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>tungstate(in) + ATP + H2O = tungstate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:35027"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46502"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.3.2.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">The complex is composed of two ATP-binding proteins (WtpC), two transmembrane proteins (WtpB) and a solute-binding protein (WtpA).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the ABC transporter superfamily. Sulfate/tungstate importer (TC 3.A.1.6) family.</text>
    </comment>
    <dbReference type="EC" id="7.3.2.6" evidence="15"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323533.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXK8"/>
    <dbReference type="STRING" id="348780.NP_3648A"/>
    <dbReference type="EnsemblBacteria" id="CAI49915">
        <property type="protein sequence ID" value="CAI49915"/>
        <property type="gene ID" value="NP_3648A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702440"/>
    <dbReference type="KEGG" id="nph:NP_3648A"/>
    <dbReference type="eggNOG" id="arCOG00177">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015408">
        <property type="term" value="F:ABC-type ferric iron transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901238">
        <property type="term" value="F:ABC-type tungstate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03259">
        <property type="entry name" value="ABC_Carb_Solutes_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000425">
        <property type="entry name" value="Probable ABC transporter, ATP-binding subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050093">
        <property type="entry name" value="ABC_SmlMolc_Importer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015853">
        <property type="entry name" value="ABC_transpr_FbpC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013611">
        <property type="entry name" value="Transp-assoc_OB_typ2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42781">
        <property type="entry name" value="SPERMIDINE/PUTRESCINE IMPORT ATP-BINDING PROTEIN POTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42781:SF1">
        <property type="entry name" value="THIAMINE IMPORT ATP-BINDING PROTEIN THIQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08402">
        <property type="entry name" value="TOBE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="MOP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0410">Iron transport</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="19">
        <location>
            <begin position="24"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00039025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00041133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00057369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAI49915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="384" mass="41622" checksum="15DA3C2C3FE4DDC1" modified="2017-05-10" version="1">MSSELKRTDQSAGPAAAAAGQTVLELDDVQKSYDGMDVIRDLSLDVRDGELLTLLGPSGCGKTTTLRLIAGLERPDNGTVRLGSTPVSGSEFVPPEQRDVGVVFQEFALFPHLSARENIAFGIQEWSEAEQQERVDELLELVGLVDHGDKSPEELSGGQQQRVALARSLAPEPKLLLLDEPFSNLDVDLRVEMREEVRDILKEAGVTAISVTHDQEEAMSISDRVAVMNNGRIEQVGTPEGVFQQPKSRFVAGFLGHASFVSGYVEDGCVETGVGCVPLEQVNGLTDVYDGSEIDLMVRPDDVCARPASPEEANGEVVHRRYLGPTVLYRVEIESGDVVGCMHNHADEVSLDDPVFVSIDADHELAWFPRGERKMADGGPDSTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXP3</accession>
    <name>A0A1U7EXP3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3796A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323606.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXP3"/>
    <dbReference type="EnsemblBacteria" id="CAI49989">
        <property type="protein sequence ID" value="CAI49989"/>
        <property type="gene ID" value="NP_3796A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701867"/>
    <dbReference type="KEGG" id="nph:NP_3796A"/>
    <dbReference type="eggNOG" id="arCOG03920">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032880_0_0_2"/>
    <dbReference type="OrthoDB" id="169315at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045466">
        <property type="entry name" value="DUF6498"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20108">
        <property type="entry name" value="DUF6498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="210"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="281"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="310"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="414" mass="45129" checksum="0BACE5FF3D601489" modified="2017-05-10" version="1">MRGRPRPGRRSSRAALVVSNLVPLVAAVLLEWSLTVLLGIYVLELYAVLFWTAVKLPFAERTVVLDEEPTETRFRLFDRGWSTDLEEKRGGIRLFGWLPPVYPRNIPAFVVFTVFVAPLAALVAFIVFALSQPTITTEVVSLLLLGGLAVFLSRGVSFVTEYLWQGGYRDHSTRSLISGPFRYLAGFGVLMIVLSAAVTEASNPDGVSTAAVLVVVVAGKITAELYFYWRDHTEGGRPLFERFLDGDERRPSLPEPPDADPTAILRPRRLAVAGGGLRRGVAYLFVGGDGSLGVLFVGACLLAALVSPGAVLVVAAGVGIPVVALRAFVYWLRYGPLEYRLYADVVIEHDRLLAAPQRRLPRGAVGDVATHEPKLPQLLANESVELRPAGWSDAGPVTARHLPDGERLREWADG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXQ6</accession>
    <name>A0A1U7EXQ6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable S-adenosylmethionine-dependent methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3790A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323603.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXQ6"/>
    <dbReference type="STRING" id="348780.NP_3790A"/>
    <dbReference type="EnsemblBacteria" id="CAI49986">
        <property type="protein sequence ID" value="CAI49986"/>
        <property type="gene ID" value="NP_3790A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701865"/>
    <dbReference type="KEGG" id="nph:NP_3790A"/>
    <dbReference type="eggNOG" id="arCOG01773">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090236_1_0_2"/>
    <dbReference type="OrthoDB" id="1018at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF24">
        <property type="entry name" value="2-METHOXY-6-POLYPRENYL-1,4-BENZOQUINOL METHYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="1">
        <location>
            <begin position="43"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="191" mass="20294" checksum="BE9CD5232A690B7D" modified="2017-05-10" version="1">MRWDVQAFDLFAPLYDIWMPSADETALRKGLACADREGDRIIDVGGGSGRAARTVGATVVDPAAGMLARARAKGLETVQASATDLPHPDDSVDAVIVVDALHHFPDPEAGLAEISRVLAPGGVLVVREFDRSTRRGRLLEAGEHLFGFESTFYTAAELEAAIEAAGLEARPVEYGFETTVCGVAPAGRDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXR1</accession>
    <name>A0A1U7EXR1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ferredoxin (2Fe-2S)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ferA5</name>
        <name evidence="2" type="ordered locus">NP_3792A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49987.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323604.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXR1"/>
    <dbReference type="STRING" id="348780.NP_3792A"/>
    <dbReference type="EnsemblBacteria" id="CAI49987">
        <property type="protein sequence ID" value="CAI49987"/>
        <property type="gene ID" value="NP_3792A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702705"/>
    <dbReference type="KEGG" id="nph:NP_3792A"/>
    <dbReference type="eggNOG" id="arCOG02987">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082632_10_3_2"/>
    <dbReference type="OrthoDB" id="180223at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="1">
        <location>
            <begin position="5"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49987.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="113" mass="12379" checksum="20F6227BFBAFE70A" modified="2017-05-10" version="1">MGDDIELTVITAADESTITVERGSVLRDVLLERGFSVYGTVSSRVNCGGRGLCATCTVEVDPPPEPAHWHDAAAVRFGYPRLSCCIEVKEPMTVRLLDKHVWGQVLPRRPDGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXU3</accession>
    <name>A0A1U7EXU3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3902A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323659.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXU3"/>
    <dbReference type="EnsemblBacteria" id="CAI50042">
        <property type="protein sequence ID" value="CAI50042"/>
        <property type="gene ID" value="NP_3902A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701911"/>
    <dbReference type="KEGG" id="nph:NP_3902A"/>
    <dbReference type="eggNOG" id="ENOG502N5A0">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2340261_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="97" mass="11108" checksum="E4108F4F6DBC4FAD" modified="2017-05-10" version="1">MASVPFVMRRMLKTCRSEERLPSDEEFKANKPAYKAALKAARDLAGEEPLDDIGEWAVEWTKETGELPTPERFRKQTRQFLTQRGIEIPEDSVLATP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXY6</accession>
    <name>A0A1U7EXY6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">Phosphoribosylformylglycinamidine cyclo-ligase</fullName>
            <ecNumber evidence="2 8">6.3.3.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">AIR synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">AIRS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Phosphoribosyl-aminoimidazole synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 11" type="primary">purM</name>
        <name evidence="11" type="ordered locus">NP_3940A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ATP = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the AIR synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.1" evidence="2 8"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323678.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXY6"/>
    <dbReference type="STRING" id="348780.NP_3940A"/>
    <dbReference type="EnsemblBacteria" id="CAI50061">
        <property type="protein sequence ID" value="CAI50061"/>
        <property type="gene ID" value="NP_3940A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703074"/>
    <dbReference type="KEGG" id="nph:NP_3940A"/>
    <dbReference type="eggNOG" id="arCOG00639">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047116_0_0_2"/>
    <dbReference type="OrthoDB" id="6605at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00129"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004637">
        <property type="term" value="F:phosphoribosylamine-glycine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004641">
        <property type="term" value="F:phosphoribosylformylglycinamidine cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046084">
        <property type="term" value="P:adenine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02196">
        <property type="entry name" value="PurM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.10:FF:000020">
        <property type="entry name" value="Phosphoribosylformylglycinamidine cyclo-ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00741">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004733">
        <property type="entry name" value="PurM_cligase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00878">
        <property type="entry name" value="purM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520:SF12">
        <property type="entry name" value="TRIFUNCTIONAL PURINE BIOSYNTHETIC PROTEIN ADENOSINE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520">
        <property type="entry name" value="TRIFUNCTIONAL PURINE BIOSYNTHETIC PROTEIN ADENOSINE-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PurM-like N-terminal" evidence="9">
        <location>
            <begin position="39"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="10">
        <location>
            <begin position="166"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI50061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="327" mass="34668" checksum="ED5E1C24329AB4B0" modified="2017-05-10" version="1">MTEDDSEAEGLTYAETGVDIEASEAATAALLGAVEDFEGDYAGLVDIGDRYLALATDGVGTKLLVAEALDDYSTIGIDCIAMNANDLIATGVTPVAFVDYLAVETPDDETAEQVGEGLSEGAERAEIALLGGETAVMPDVIRGLDLAGACVGLATEDELFDGEAAAGDYLVGLPSSGIHSNGLTLAREAVTQRHDYTDPFPYDDDRTIGEVLLEPTRIYREVLGPLHAAETHAAAHVTGGGWTNLSRMGEYRYVIEDPFEPQPVFEFVQEEGSVDDEEMHRTFNMGTGFVAALPADDAEAVVEELSDARIIGRVEDGDAVEIRGLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXZ9</accession>
    <name>A0A1U7EXZ9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">ATP-dependent DNA helicase</fullName>
            <ecNumber evidence="12">3.6.4.12</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">lhr</name>
        <name evidence="12" type="ordered locus">NP_4002A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="9">Belongs to the Lhr helicase family. Lhr-Core subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="12"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50092.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939648.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXZ9"/>
    <dbReference type="STRING" id="348780.NP_4002A"/>
    <dbReference type="EnsemblBacteria" id="CAI50092">
        <property type="protein sequence ID" value="CAI50092"/>
        <property type="gene ID" value="NP_4002A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702884"/>
    <dbReference type="KEGG" id="nph:NP_4002A"/>
    <dbReference type="eggNOG" id="arCOG00557">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002025_0_0_2"/>
    <dbReference type="OrthoDB" id="33870at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17922">
        <property type="entry name" value="DEXHc_LHR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052511">
        <property type="entry name" value="ATP-dep_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017170">
        <property type="entry name" value="Lhr-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013701">
        <property type="entry name" value="Lhr-like_DEAD/DEAH_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045628">
        <property type="entry name" value="Lhr_WH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47962:SF5">
        <property type="entry name" value="ATP-DEPENDENT HELICASE LHR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47962">
        <property type="entry name" value="ATP-DEPENDENT HELICASE LHR-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08494">
        <property type="entry name" value="DEAD_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19306">
        <property type="entry name" value="WHD_Lhr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037307">
        <property type="entry name" value="Lhr-like_helic_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="41"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="259"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00093467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI50092.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="948" mass="103693" checksum="8E848EC72EE34377" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY35</accession>
    <name>A0A1U7EY35_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4088A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323751.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY35"/>
    <dbReference type="STRING" id="348780.NP_4088A"/>
    <dbReference type="EnsemblBacteria" id="CAI50135">
        <property type="protein sequence ID" value="CAI50135"/>
        <property type="gene ID" value="NP_4088A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701967"/>
    <dbReference type="KEGG" id="nph:NP_4088A"/>
    <dbReference type="eggNOG" id="arCOG06949">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_648305_0_0_2"/>
    <dbReference type="OrthoDB" id="205807at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="427" mass="46071" checksum="8A8CD933BC7838B4" modified="2017-05-10" version="1">MSPRRRTVLAAVGSTAAALSAGVGYWQRHRLRRRDETDAIEAALGIDPPAVETSVAVTDAHLHASYSRARDHIEETASLLDSAADASGTQHSRRARAQLDDAHEDLDASRPSELTDDEVPLEILTTYRLAIAGSAWARSVVHDGSAGPPSEALHDASEALRADLDGIEPQYRGSSLSAAVVQCGEADEMCAVAASRASRAQRFLDDSEYSHAVTWEVVETARQTVYDADWLFDEQDGRDRTGALADAFERLTDRVDTDTENVTSEYEDGVRSHAAARLTDRRLSGTAPETHFDAGRPALAVREQAQTAMIAATLDSLAGFPSTRALDGSEHVLTDDSEPLLAAKRDATEALTAAADEAGVDPLGRYLLAEAIDTADRGDRQLTRLLDNVNSYDHEAWVSRRDTAYLRFRAAAAEARAVPDVVELVNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY46</accession>
    <name>A0A1U7EY46_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GalE family epimerase/dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4112A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323763.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY46"/>
    <dbReference type="STRING" id="348780.NP_4112A"/>
    <dbReference type="EnsemblBacteria" id="CAI50147">
        <property type="protein sequence ID" value="CAI50147"/>
        <property type="gene ID" value="NP_4112A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702735"/>
    <dbReference type="KEGG" id="nph:NP_4112A"/>
    <dbReference type="eggNOG" id="arCOG01369">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_7_2"/>
    <dbReference type="OrthoDB" id="4907at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050177">
        <property type="entry name" value="Lipid_A_modif_metabolic_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43245">
        <property type="entry name" value="BIFUNCTIONAL POLYMYXIN RESISTANCE PROTEIN ARNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43245:SF23">
        <property type="entry name" value="NAD(P)-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="1">
        <location>
            <begin position="8"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="313" mass="33984" checksum="435E76E5983CF3C2" modified="2017-05-10" version="1">MQLDGKRVCITGGGGFVGSHLAERLVDANDVVVVDRFSNGRREWVPDAADIVAGDLRDQTVRAAAITDDTDLVFHLAADKAVDSDDPDEQFRLNTDLTAGVLERMRSVGCDAIAFTSSSTVYGEAPRPTPEDYAPLEPISMYGAAKLAEESLLSVHAHSHGMDAWVFRFANIVGPRLQPGAVIPDFIEKLREDPTTLEILGDGRQRKSYMHIDDCVDAMCHIVETADGDGLNTYNLGTRTTTAVRRIADIVADELGADPTYEYTGGDRGWVGDVPRMRLAIEKLSALGWDPSQSSDEAVRQAASELSAVSFER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY55</accession>
    <name>A0A1U7EY55_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DUF2797 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4116A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50149.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939855.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY55"/>
    <dbReference type="STRING" id="348780.NP_4116A"/>
    <dbReference type="EnsemblBacteria" id="CAI50149">
        <property type="protein sequence ID" value="CAI50149"/>
        <property type="gene ID" value="NP_4116A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701978"/>
    <dbReference type="KEGG" id="nph:NP_4116A"/>
    <dbReference type="eggNOG" id="arCOG03129">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073248_0_0_2"/>
    <dbReference type="OrthoDB" id="45375at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021246">
        <property type="entry name" value="DUF2797"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10977">
        <property type="entry name" value="DUF2797"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50149.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="255" mass="27541" checksum="955C8F1692707B89" modified="2017-05-10" version="1">MQVVGYETGVGDDEPPALQVAVDGAVSREPLAPGRELEYSLGQRRCAGVVDGDDHVACDASAAPYCQAHDSTWVCARCTGTCLKAEMDCHQEHAIYLAAFAPSTFKVGVTRRSDPRVRLREQGADRGAIIRRVSNGRIAREIEADIAASEPLSDVVRTAVKVAGLGKPIDETAWKRLLSRFEVSARHSFDYGLGLDATPVAETLAAGTVRGTKGRILVLDRGDDTFAVDMRALVGYEVRSEPPSRRLQSSLGRFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYA7</accession>
    <name>A0A1U7EYA7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Aliphatic amidase</fullName>
            <ecNumber evidence="4">3.5.1.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">amiE</name>
        <name evidence="4" type="ordered locus">NP_4212A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.1.4" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323813.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYA7"/>
    <dbReference type="STRING" id="348780.NP_4212A"/>
    <dbReference type="EnsemblBacteria" id="CAI50197">
        <property type="protein sequence ID" value="CAI50197"/>
        <property type="gene ID" value="NP_4212A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702496"/>
    <dbReference type="KEGG" id="nph:NP_4212A"/>
    <dbReference type="eggNOG" id="arCOG00062">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071797_0_0_2"/>
    <dbReference type="OrthoDB" id="39312at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004040">
        <property type="term" value="F:amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07565">
        <property type="entry name" value="aliphatic_amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050345">
        <property type="entry name" value="Aliph_Amidase/BUP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009802">
        <property type="entry name" value="PRK13286.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43674:SF14">
        <property type="entry name" value="ALIPHATIC AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43674">
        <property type="entry name" value="NITRILASE C965.09-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="3">
        <location>
            <begin position="13"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="312"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="350" mass="38523" checksum="CCD78EE4242786B0" modified="2017-05-10" version="1">MRHGDISSSGDTVGVAVHQTAAPSPETKDEVLEQCENIASKIEGIKTGAPGVDLVIFPEYSTQGITYDEELMMELATEVPGPETEIFSRACDEHDVWGVFSITGEKHEDHPERNPYNTLILIDDEGEIRQTYRKIIPWCPIEGWTAGDQTYVTEGPKGLNISLIICDDGNYPEIWRDCAYKGAELIVRCQGYMYPAAEQQELVARHAAWANNAYVAVSNLAGSDEVYSFFGNSNIVGFDGRVLGSCGSDENVWQYAELSVSAIRDARANWQSQNHLYKLLHRGYTSTEDAGLGDGEAENPFDFYDDWVENPEKAAEQIESITRSTPGVEEAPVEGVPTETAEPPEAAGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYD2</accession>
    <name>A0A1U7EYD2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Probable oxidoreductase (Short-chain dehydrogenase family)</fullName>
            <ecNumber evidence="1">1.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4278A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323846.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYD2"/>
    <dbReference type="STRING" id="348780.NP_4278A"/>
    <dbReference type="EnsemblBacteria" id="CAI50230">
        <property type="protein sequence ID" value="CAI50230"/>
        <property type="gene ID" value="NP_4278A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702017"/>
    <dbReference type="KEGG" id="nph:NP_4278A"/>
    <dbReference type="eggNOG" id="arCOG01259">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_2_2"/>
    <dbReference type="OrthoDB" id="312184at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05338">
        <property type="entry name" value="DHRS1_HSDL2-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051935">
        <property type="entry name" value="HSDL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006133">
        <property type="entry name" value="PRK08278.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42808">
        <property type="entry name" value="HYDROXYSTEROID DEHYDROGENASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42808:SF3">
        <property type="entry name" value="HYDROXYSTEROID DEHYDROGENASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="288" mass="31312" checksum="C49203C1F10A0FEE" modified="2017-05-10" version="1">MSVDAPDLSGQTAFITGTTRGIGKAIALALAEQGCNIVSTGKTSEADDYGEDKDLEGTIEQTARECEEKGVEALPIQLNVRDEDRVEAAVEEAIDHFGEVNIVINNASAIQLANVEDLPANRFDLLTDVNVRGTYLVSRAFMDHLKQTDEDAWILTNAPPVTVDRAPGEAPYAWSKMGMSFLTLSLATELSGHDIGCNSFWPVTAIDTRATRYFGLGTEDDWRSPDIVSDTVLEILSRDPASYTGNAVYDEELLAAAGVEDFSEYNLTEGNPAPMSAQMFDPDYERSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYF3</accession>
    <name>A0A1U7EYF3_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sec-independent protein translocase protein TatA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">tatA2</name>
        <name evidence="9" type="synonym">tatA</name>
        <name evidence="11" type="ordered locus">NP_4350A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes. TatA could form the protein-conducting channel of the Tat system.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Forms a complex with TatC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the TatA/E family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323882.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYF3"/>
    <dbReference type="STRING" id="348780.NP_4350A"/>
    <dbReference type="EnsemblBacteria" id="CAI50266">
        <property type="protein sequence ID" value="CAI50266"/>
        <property type="gene ID" value="NP_4350A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703226"/>
    <dbReference type="KEGG" id="nph:NP_4350A"/>
    <dbReference type="eggNOG" id="arCOG02694">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086034_3_3_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033281">
        <property type="term" value="C:TAT protein transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043953">
        <property type="term" value="P:protein transport by the Tat complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.3310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00236">
        <property type="entry name" value="TatA_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003369">
        <property type="entry name" value="TatA/B/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006312">
        <property type="entry name" value="TatA/E"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01411">
        <property type="entry name" value="tatAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42982">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42982:SF1">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02416">
        <property type="entry name" value="TatA_B_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="41"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="86"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI50266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="111" mass="11723" checksum="64B865DD4F8BD9E7" modified="2017-05-10" version="1">MTTLLFIGGLGPMEIALIAGVLVLLFGASKLPELARSMGTATGEFQKGREEVEQELQEMRDGESTTGDETVDDDEADIKSASDAASEPDVETEPETESETEPETDPDGSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYI7</accession>
    <name>A0A1U7EYI7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Fumarylacetoacetase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">hpcE2</name>
        <name evidence="2" type="ordered locus">NP_4386A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323900.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYI7"/>
    <dbReference type="STRING" id="348780.NP_4386A"/>
    <dbReference type="EnsemblBacteria" id="CAI50284">
        <property type="protein sequence ID" value="CAI50284"/>
        <property type="gene ID" value="NP_4386A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702798"/>
    <dbReference type="KEGG" id="nph:NP_4386A"/>
    <dbReference type="eggNOG" id="arCOG00235">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028458_3_3_2"/>
    <dbReference type="OrthoDB" id="38993at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="entry name" value="Fumarylacetoacetase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43211:SF1">
        <property type="entry name" value="BLL6422 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43211">
        <property type="entry name" value="FUMARYLACETOACETATE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="FAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarylacetoacetase-like C-terminal" evidence="1">
        <location>
            <begin position="115"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="319" mass="34469" checksum="B4299761BBB77C17" modified="2017-05-10" version="1">MKLATFEVETPVGPVRRLGVPHDDELIDIHSAYAAALAADGEADPVSIADAHAPPDIIEFLRRGDRSREAAQEAVAYADEAAIERGPTGGRLRFDADEVSLLAPVPRPNSLRDFMVFEEHVRNALGEEPPDAWYEMPVYYKSTADSVVAPGETVEWPAYSSLMDYELELAAVIGKEGRDIPAEAADEYIAGYTIFNDFSARDIQGEEMQARLGPAKGKDFANAFGPYLVTADDFDPETAEMRATVNGEVWSEGTPGEMYHTFADIVAHVSQSETLYPGDVLGSGTVGEGCGLELGQFLESGDTVSLFVDGIGTLENTVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYI9</accession>
    <name>A0A1U7EYI9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">CytC-type peroxidase</fullName>
            <ecNumber evidence="10">1.11.1.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">cpx</name>
        <name evidence="10" type="ordered locus">NP_4384A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell envelope</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.11.1.5" evidence="10"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323899.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYI9"/>
    <dbReference type="STRING" id="348780.NP_4384A"/>
    <dbReference type="EnsemblBacteria" id="CAI50283">
        <property type="protein sequence ID" value="CAI50283"/>
        <property type="gene ID" value="NP_4384A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702629"/>
    <dbReference type="KEGG" id="nph:NP_4384A"/>
    <dbReference type="eggNOG" id="arCOG06268">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034652_3_1_2"/>
    <dbReference type="OrthoDB" id="202054at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004130">
        <property type="term" value="F:cytochrome-c peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="entry name" value="Cytochrome c-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051395">
        <property type="entry name" value="Cytochrome_c_Peroxidase/MauG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004852">
        <property type="entry name" value="Di-haem_cyt_c_peroxidsae"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30600">
        <property type="entry name" value="CYTOCHROME C PEROXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30600:SF7">
        <property type="entry name" value="CYTOCHROME C PEROXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03150">
        <property type="entry name" value="CCP_MauG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="Cytochrome c"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0349">Heme</keyword>
    <keyword evidence="5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="427"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="9">
        <location>
            <begin position="75"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="9">
        <location>
            <begin position="236"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="396"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI50283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="453" mass="50016" checksum="FF4FF1E59F72ADB0" modified="2017-05-10" version="1">MLNRRRQLFVALGVALMLVLGAGAVAAADDVESDTGDVSQVQQMDNETTDLPVEPPTEVSHPGYEYIRQEGDTEEKIELGEQLFLDPRISETGTISCNTCHNVMEGGDDSRPTAMGVHGQTGPVASPTVWNSGFHHTQFWDGRADTLAEQAEGPLVADVEMGMPDHEAALDRIRSVPGYVESYEEVYGDEAESTDPDDLITLERTVDAIAAYERTLVTPNSPYDQYVEGDEDALTEQQLDGMETFQELGCQSCHSGPMFSGQWEEPESGEGVYQPHPTFRENEQCQEYVEEYGLMDNPGRMGVTDDAADEFLYKVPTLRNVEHTAPYMHTGQVETLEDAIRVMAACSLDEEVSDEEVEDIHAFLTSLTGEYPDQEMPRLPNPSGESMIPMDADTEFEDMEEEPEEDMENDIEENGADDEEVEDTPGFTIGAAVLALALVLATALIGYNRRTEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYK6</accession>
    <name>A0A1U7EYK6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4428A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323920.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYK6"/>
    <dbReference type="EnsemblBacteria" id="CAI50305">
        <property type="protein sequence ID" value="CAI50305"/>
        <property type="gene ID" value="NP_4428A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702060"/>
    <dbReference type="KEGG" id="nph:NP_4428A"/>
    <dbReference type="HOGENOM" id="CLU_1933316_0_0_2"/>
    <dbReference type="OrthoDB" id="223969at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="130" mass="13834" checksum="B245FF480AA170F4" modified="2017-05-10" version="1">MADDDGVRALLLDHSDQQAVRNVFEAYTGTGSATLTDYVTAMRATGGDLAVVASDGAADIYACWNGSGGRYEHLTVWPPWSIGGYDHADRAGLESVLDATDDLRVIPHSETPFAEPGTLGSLSHRTELQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYL0</accession>
    <name>A0A1U7EYL0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF7978 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_4442A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323927.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYL0"/>
    <dbReference type="STRING" id="348780.NP_4442A"/>
    <dbReference type="EnsemblBacteria" id="CAI50312">
        <property type="protein sequence ID" value="CAI50312"/>
        <property type="gene ID" value="NP_4442A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702064"/>
    <dbReference type="KEGG" id="nph:NP_4442A"/>
    <dbReference type="eggNOG" id="arCOG06413">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100471_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058284">
        <property type="entry name" value="DUF7978"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25933">
        <property type="entry name" value="DUF7978"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="40"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="216"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7978" evidence="3">
        <location>
            <begin position="26"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="250" mass="25211" checksum="B0BE29B76337CA06" modified="2017-05-10" version="1">MPPEPLQSNGSCPEQTADSETPSGGQPDTNRHRPKALGRIVYVCAQAGSAAFFVGIALAGAVVRLREPERQLAEIDAPKSAFSTLPDTTEALLWTLFQAHGVDITVRESSTAPETSAAIADAAIVDPSWLVVAVPLVLLAAGGAATAALAPQSLWGAAGTGAAITVAYAPLAALAVWLATYSPSTVTMGIEGDPGPGTAVFGDGWAVGFSIGPDPLAAVFVAGIAYPVVFGAVGGAAAHLKKQSRSEPSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYP2</accession>
    <name>A0A1U7EYP2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable S-adenosylmethionine-dependent methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4574A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50378.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939684.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYP2"/>
    <dbReference type="STRING" id="348780.NP_4574A"/>
    <dbReference type="EnsemblBacteria" id="CAI50378">
        <property type="protein sequence ID" value="CAI50378"/>
        <property type="gene ID" value="NP_4574A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702102"/>
    <dbReference type="KEGG" id="nph:NP_4574A"/>
    <dbReference type="eggNOG" id="arCOG03529">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1154328_0_0_2"/>
    <dbReference type="OrthoDB" id="11691at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="1">
        <location>
            <begin position="48"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50378.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="243" mass="26374" checksum="1F6ED4D04B2DB7D6" modified="2017-05-10" version="1">MKNAIRTNFDESEQAYERYENATGRFAALADRLAGQLASDGDGLGRTLDAGAGNGHSTRVFAEESKRLVALDISRGMLRANPTADRVQADFDALPFRAERFDTVAFTASLFLTPSPETAAGEAARVLRADGTVGAVAPLGWTTPAGDDVFGPLERDSRSPTGAEAVVDALRRHFSVETGTWTFETTAEALRQFHAIPAMAARLYPRLDADARVERAQELLEAVEGPLEQRWQWIVGEPKPASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYR4</accession>
    <name>A0A1U7EYR4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">AI-2E family transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">NP_4576A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323994.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYR4"/>
    <dbReference type="STRING" id="348780.NP_4576A"/>
    <dbReference type="DNASU" id="3702103"/>
    <dbReference type="EnsemblBacteria" id="CAI50379">
        <property type="protein sequence ID" value="CAI50379"/>
        <property type="gene ID" value="NP_4576A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702103"/>
    <dbReference type="KEGG" id="nph:NP_4576A"/>
    <dbReference type="eggNOG" id="arCOG02642">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048845_0_0_2"/>
    <dbReference type="OrthoDB" id="282734at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002549">
        <property type="entry name" value="AI-2E-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 245"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01594">
        <property type="entry name" value="AI-2E_transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="59"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="241"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="272"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="310"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI50379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="369" mass="40091" checksum="03A33434C31594D6" modified="2017-05-10" version="1">MDEKRLAVAAFGVLVAAVVAYVAARFIAALTVAVFLYYSTRRFYALLGRLRLPKRIRAVLALSVLGVPLMLLISYTVILLAVEARQFVEEYPVFESAPDDVAWFEGLEDLPEPTINGIIQAYQAGDLDIFIDFAVEHAAFLSSLITGLLLNLLIVVVVTYYLLIDGTKARDWLLRFDEDAIVREYLEAVDDELEAVLFGNLLNVIVISLIAVAVFMGYNAAVPAPVEVPYPALAGALTGVASLIPVVGMKIVYLPLAVAAAVPVVLDGPTELLVYIVGFLALAVIVVDTIPDLVLRPYFSGNRTHVGLLMLAYIFGPVVFGFYGLFFAPILLVLGLTFADTALPRLLGVDPDDGRSIAPSQATLDRFRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYS7</accession>
    <name>A0A1U7EYS7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UspA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_4592A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324002.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYS7"/>
    <dbReference type="STRING" id="348780.NP_4592A"/>
    <dbReference type="EnsemblBacteria" id="CAI50387">
        <property type="protein sequence ID" value="CAI50387"/>
        <property type="gene ID" value="NP_4592A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702108"/>
    <dbReference type="KEGG" id="nph:NP_4592A"/>
    <dbReference type="eggNOG" id="arCOG00449">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_2_0_2"/>
    <dbReference type="OrthoDB" id="107030at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF6">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN UP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="2"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="150"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="283" mass="31019" checksum="7F267FDA3146D02C" modified="2017-05-10" version="1">MKAVCATDLSAASEAAIESETCLRCLSRIGVEKLHLVTVVPSNVHTGIPGLNFEERRQSALSRYQSVLESAGFEVEAHAVRGTPYRRINGVAEAVDADLIIVGSRGESPLENRAIGSTARNLARTAVAPLLVNRVEEATDDPEVVREHLFQHTLYATDFSSNAEQAFETFSYLHPAVQDATLVHVQTPKDPDPEDDDPEERLAALADRLREWDIDTQTDVRYGDPADEILAAEADHEPTMVLLGSRGHGRLRRLLLGSVSEEIVAHADGNVLLVPPERPRQPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYV7</accession>
    <name>A0A1U7EYV7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Phosphohexomutase (Phosphoglucomutase / phosphomannomutase)</fullName>
            <ecNumber evidence="12">5.4.2.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">pmm1</name>
        <name evidence="12" type="ordered locus">NP_4660A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.-" evidence="12"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324035.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYV7"/>
    <dbReference type="STRING" id="348780.NP_4660A"/>
    <dbReference type="EnsemblBacteria" id="CAI50421">
        <property type="protein sequence ID" value="CAI50421"/>
        <property type="gene ID" value="NP_4660A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703035"/>
    <dbReference type="KEGG" id="nph:NP_4660A"/>
    <dbReference type="eggNOG" id="arCOG00767">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016950_7_1_2"/>
    <dbReference type="OrthoDB" id="10363at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008966">
        <property type="term" value="F:phosphoglucosamine mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03087">
        <property type="entry name" value="PGM_like1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024086">
        <property type="entry name" value="GlmM_arc-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03990">
        <property type="entry name" value="Arch_GlmM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43771">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43771:SF1">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="Phosphoglucomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="9">
        <location>
            <begin position="2"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="10">
        <location>
            <begin position="149"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="11">
        <location>
            <begin position="248"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase C-terminal" evidence="8">
        <location>
            <begin position="375"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI50421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="439" mass="45778" checksum="1491A7D0214B89CD" modified="2017-05-10" version="1">MFGTSGIRGPFGETVTAELALDIGRALTTAGYNTIVVGRDPRTTGPLLVDALSAGIREGGGNVINVGVASTPTVARSVGWRAADAGVSVTASHNPPEDNGIKLWSPSGMAFDEAQRSEIETLLASDRFESAAWDAVGSEERWEAATEQHITAIADTVPQVEDVHAVVDVGNGAGDLTIDALRALGCTVDTLNARPDGHFPSRPSEPTAEACTALQAHVEATAADIGIAHDGDGDRMMAVDETGRFVGGDELLALFGRAVATEGDRIAAPLNTSQLVDAALAEVGAELVRTRVGDVYVAERAREADVVFGGEPSGAWIWPTTTLAPDGPLAAARAVELVATNGPLSEQVASLEQYPLRRESIPVDDKRAVMDGVTEAATDRYDTVQQLDGVRIERDDGWMLIRASGTQPLVRLTAEGTTDADAASLLATARDIVASARPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYW8</accession>
    <name>A0A1U7EYW8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Halocyanin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">hcp7</name>
        <name evidence="10" type="ordered locus">NP_4692A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324051.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYW8"/>
    <dbReference type="STRING" id="348780.NP_4692A"/>
    <dbReference type="EnsemblBacteria" id="CAI50437">
        <property type="protein sequence ID" value="CAI50437"/>
        <property type="gene ID" value="NP_4692A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702145"/>
    <dbReference type="KEGG" id="nph:NP_4692A"/>
    <dbReference type="eggNOG" id="arCOG02920">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_716924_0_0_2"/>
    <dbReference type="OrthoDB" id="11088at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04220">
        <property type="entry name" value="Halocyanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000923">
        <property type="entry name" value="BlueCu_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025512">
        <property type="entry name" value="DUF4399"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34192">
        <property type="entry name" value="PLASTOCYANIN MAJOR ISOFORM, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34192:SF10">
        <property type="entry name" value="PLASTOCYANIN MAJOR ISOFORM, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00127">
        <property type="entry name" value="Copper-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14347">
        <property type="entry name" value="DUF4399"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0186">Copper</keyword>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DUF4399" evidence="9">
        <location>
            <begin position="92"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4399" evidence="9">
        <location>
            <begin position="213"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Blue (type 1) copper" evidence="8">
        <location>
            <begin position="303"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="23"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="28"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI50437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="385" mass="40047" checksum="6F03482E36FEA77D" modified="2017-05-10" version="1">MAPPRRRQILSIGGLGLAAAIAGCGDTAPDDDTPESVDDDETDATPEPDDDDEPAAGDEAADEDQPTNVAADAAVSFEAPLDGETAANGVAVSMTATNIEIQPAGEATDNAGYFSISIGEGATDPGEPVPHGDAHVHYEDGDTRDVLELPVGTHELTLQAADGEGRALPLTDTVAVSVESGSFTIADPAADEPATSPVSFAFEPSGAVTTAAAETPLSEAGQTAGYFVVGFGTGATPTGEQIPRTDAVVHLDDGATTADIERSPGEYELTVQMATPDGRALPATDTMSLTVVEAPETVIVGPGGTQNFEPEALTVSSGTTVRFVWEGDFHNIEVRSQPDDSQWEGVPETRNEGFEYEHTFEVPGVYEYRCEPHRLSMQGTITVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYY3</accession>
    <name>A0A1U7EYY3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">YdjM family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_4792A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324099.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYY3"/>
    <dbReference type="STRING" id="348780.NP_4792A"/>
    <dbReference type="EnsemblBacteria" id="CAI50487">
        <property type="protein sequence ID" value="CAI50487"/>
        <property type="gene ID" value="NP_4792A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702177"/>
    <dbReference type="KEGG" id="nph:NP_4792A"/>
    <dbReference type="eggNOG" id="arCOG04605">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081182_0_0_2"/>
    <dbReference type="OrthoDB" id="252570at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007404">
        <property type="entry name" value="YdjM-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04307">
        <property type="entry name" value="YdjM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="54"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="220" mass="24136" checksum="BFD9F88EBA219566" modified="2017-05-10" version="1">MPSTVVHAAVAFLLAVGLLGRFYDRRALLVVLLVILFPELDTALGWVMDGAHRTVLHTMMLSVTAAAVLYWETVRDGSWLRDRFGDYGIRVAWVALVAHTVAHIALDWSHLSGINFFWPLRDTFYALDGELYLSASDGVVQTFVDIEFGDETGAAVDAGRTGTTADTHVGNPAQPSSALEDGPVDRRFPIAVGGWQLYLVCLGAFALVARRLQTPRYDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ04</accession>
    <name>A0A1U7EZ04_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7978 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_4784A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324095.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ04"/>
    <dbReference type="EnsemblBacteria" id="CAI50483">
        <property type="protein sequence ID" value="CAI50483"/>
        <property type="gene ID" value="NP_4784A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702174"/>
    <dbReference type="KEGG" id="nph:NP_4784A"/>
    <dbReference type="eggNOG" id="arCOG06413">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100471_0_0_2"/>
    <dbReference type="OrthoDB" id="270777at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058284">
        <property type="entry name" value="DUF7978"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25933">
        <property type="entry name" value="DUF7978"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7978" evidence="2">
        <location>
            <begin position="6"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="185" mass="18794" checksum="5E10FA222FDD205B" modified="2017-05-10" version="1">MTPATDADSKPFIEAAVTGAVAWVLGYVFTYLIVSGDIEESPLNQLIEAFEGEQATYEIVGWVFYNAHLVDVSYSGLGPLAPPQNFVGGDGFTPFLYVIPPALLVISGLAIARYTGASDVNDGAISGLLVVPGYLVLAALGTVLFSISVGGVSGAPDLAPAVLIAGVVYPAVFGALGGVVASVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ16</accession>
    <name>A0A1U7EZ16_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Small ribosomal subunit protein eS8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">rps8e</name>
        <name evidence="8" type="ordered locus">NP_4798A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2 6">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the eukaryotic ribosomal protein eS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324102.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ16"/>
    <dbReference type="STRING" id="348780.NP_4798A"/>
    <dbReference type="EnsemblBacteria" id="CAI50490">
        <property type="protein sequence ID" value="CAI50490"/>
        <property type="gene ID" value="NP_4798A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703188"/>
    <dbReference type="KEGG" id="nph:NP_4798A"/>
    <dbReference type="eggNOG" id="arCOG04154">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080597_2_1_2"/>
    <dbReference type="OrthoDB" id="372305at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11382">
        <property type="entry name" value="Ribosomal_S8e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00029">
        <property type="entry name" value="Ribosomal_eS8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001047">
        <property type="entry name" value="Ribosomal_eS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018283">
        <property type="entry name" value="Ribosomal_eS8_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020919">
        <property type="entry name" value="Ribosomal_protein_eS8_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022309">
        <property type="entry name" value="Ribosomal_Se8/biogenesis_NSA2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00307">
        <property type="entry name" value="eS8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10394">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01201">
        <property type="entry name" value="Ribosomal_S8e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01193">
        <property type="entry name" value="RIBOSOMAL_S8E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 6 8" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
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        </source>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI50490.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
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</entry>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
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                <person name="Rodewald K."/>
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            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
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        <property type="entry name" value="DUF87"/>
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            <end position="442"/>
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            <end position="33"/>
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    </feature>
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        <location>
            <begin position="10"/>
            <end position="26"/>
        </location>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF01935"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI50604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="591" mass="65439" checksum="B4A5F9A40D74DBDF" modified="2017-05-10" version="1"> </sequence>
</entry>
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    <accession>A0A1U7EZD2</accession>
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        <submittedName>
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        <name evidence="7" type="ordered locus">NP_5130A</name>
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    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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            <dbReference type="PubMed" id="16169924"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
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        </location>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50112"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50113"/>
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        <source>
            <dbReference type="PROSITE" id="PS51898"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI50656.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
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    </evidence>
    <sequence length="839" mass="90121" checksum="4897D9F71744D6A5" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZH6</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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        <name evidence="2" type="ordered locus">NP_5212A</name>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
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                <person name="Pfeiffer F."/>
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                <person name="Rodewald K."/>
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            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
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    </reference>
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    <proteinExistence type="predicted"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="252" mass="25690" checksum="0BDD393B1D08026E" modified="2017-05-10" version="1">MSTVLDVTVCLLLVGVAVVTLATAVPGAGEEPSAPDGGDAAAAELGTVTASVSSDGATRHDTLAGHLARAAVANTTVGGEPVAATGYPDAAAEAVRNRSGDRTHVTARWRPLGDASTSGTVTVGRPPPRTADVSVTTFTVESGLRPATAASDPATSLANAYVAWLFPPERTRGLLVDSRTAPAAKEQYTETAATLDTAVDAPLAEANAETVNERLSTALAERIKTVSEGHGDVDSLETPSEEVAVVVRRWEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZI2</accession>
    <name>A0A1U7EZI2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phosphodiesterase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_5246A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324324.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZI2"/>
    <dbReference type="EnsemblBacteria" id="CAI50714">
        <property type="protein sequence ID" value="CAI50714"/>
        <property type="gene ID" value="NP_5246A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702310"/>
    <dbReference type="KEGG" id="nph:NP_5246A"/>
    <dbReference type="eggNOG" id="arCOG01738">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135999_0_0_2"/>
    <dbReference type="OrthoDB" id="200286at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1140.10">
        <property type="entry name" value="Cyclic phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009097">
        <property type="entry name" value="Cyclic_Pdiesterase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13563">
        <property type="entry name" value="2_5_RNA_ligase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55144">
        <property type="entry name" value="LigT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="162" mass="17518" checksum="A28E6C343679085F" modified="2017-05-10" version="1">MYSCNVPVPADISRLARGLAAETLTATPRDRHTLVAKRLGDGDAAVLARRLRKSLDGLDPFTVHISGVEVFDRPPTGPAPVAYLAVESPALSRLHRRLCSAFDPIAGLEDDEYVPHVTVARGGDADRLAGRAVPDDAGWTVESLSLWSATYEESVERFSLPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZL3</accession>
    <name>A0A1U7EZL3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_5286A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50734.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939758.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZL3"/>
    <dbReference type="STRING" id="348780.NP_5286A"/>
    <dbReference type="EnsemblBacteria" id="CAI50734">
        <property type="protein sequence ID" value="CAI50734"/>
        <property type="gene ID" value="NP_5286A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702320"/>
    <dbReference type="KEGG" id="nph:NP_5286A"/>
    <dbReference type="HOGENOM" id="CLU_1933317_0_0_2"/>
    <dbReference type="OrthoDB" id="238522at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50734.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="130" mass="13512" checksum="5A708A92454484F6" modified="2017-05-10" version="1">MSSRLVSRRRAVAVAATVAVAGCLTEAGRRCRGNTVRLSMQPTTADDPLGLSSLGPEATGVIETARDGTHVEHCVVWEPSGDETGPSPGLAAVVGRLVDDSDRGPPAAGEELAVDVEYEQQAYRLVVAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZM3</accession>
    <name>A0A1U7EZM3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Peptidase M20 family protein (Homolog to carboxypeptidase)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_5372A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324385.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZM3"/>
    <dbReference type="STRING" id="348780.NP_5372A"/>
    <dbReference type="EnsemblBacteria" id="CAI50777">
        <property type="protein sequence ID" value="CAI50777"/>
        <property type="gene ID" value="NP_5372A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702646"/>
    <dbReference type="KEGG" id="nph:NP_5372A"/>
    <dbReference type="eggNOG" id="arCOG01107">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021802_7_0_2"/>
    <dbReference type="OrthoDB" id="24854at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03885">
        <property type="entry name" value="M20_CPDG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017150">
        <property type="entry name" value="Pept_M20_glutamate_carboxypep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050072">
        <property type="entry name" value="Peptidase_M20A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808">
        <property type="entry name" value="ACETYLORNITHINE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808:SF9">
        <property type="entry name" value="BLL0789 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037238">
        <property type="entry name" value="Carboxypeptidase_G2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="3">
        <location>
            <begin position="194"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="401" mass="43062" checksum="E2DEE83D2D6DD10F" modified="2017-05-10" version="1">MTPTGEPPALEDRLQDWSESHRDEIADYLLELVEIETPSDEPEAFDQFFDRYGGDLQAVGLETQRIQGDETGGRLEAWSAGGDEADEIQLVVGHADTVWPLGTVKENPPEIREDVLEGPGSLDMKGGLTQLVFALRALDAVDADPELPVYVLVSSDEEVGSPESKPHIIDLAKRANRIFVLEPASGPEGKIKTARKAVGRFTVTIEGQAAHAGLEPEAGASATEELSSVIRRLHALTDLDSGVTVNVGEVEAGLRSNVVAPEARAEVDVRAPTDEAAETVEKAIRSLDAETPGTELTIEGGFGRPPMERTPGNRLLWERVRTVGDRLGLALEDARVGGASDGNDASRHAPTIDGFGAVGDGAHQEFEHVRLDPLVDRVALLAACLLDEPLPDEYEQELEDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZM6</accession>
    <name>A0A1U7EZM6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DZR domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_5328A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324364.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZM6"/>
    <dbReference type="STRING" id="348780.NP_5328A"/>
    <dbReference type="EnsemblBacteria" id="CAI50755">
        <property type="protein sequence ID" value="CAI50755"/>
        <property type="gene ID" value="NP_5328A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702334"/>
    <dbReference type="KEGG" id="nph:NP_5328A"/>
    <dbReference type="eggNOG" id="arCOG01916">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098526_0_0_2"/>
    <dbReference type="OrthoDB" id="11143at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056278">
        <property type="entry name" value="CdrL_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025874">
        <property type="entry name" value="DZR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24252">
        <property type="entry name" value="CdrL_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12773">
        <property type="entry name" value="DZR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CdrL-like middle region" evidence="3">
        <location>
            <begin position="53"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="DZANK-type" evidence="2">
        <location>
            <begin position="129"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="185" mass="19916" checksum="0CCE80AFF3E3EFF7" modified="2017-05-10" version="1">MSKITFRADEDLIRQLETFDASKSEVMREALREYLDDAGGDTGGRPPTDNGGSIDDSLDDLIAERVDAILDERLGGHLGASYTPREPQDVNVNISLSGDSAEMSHGTDESRKTPAEDTAGASDTDAESCKQCGEDLSPSDVYCPNCGEKASHRVFCECGDELRSDWGFCPSCGRRTPAADVLKDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZN2</accession>
    <name>A0A1U7EZN2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CopG domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5326A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324363.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZN2"/>
    <dbReference type="STRING" id="348780.NP_5326A"/>
    <dbReference type="EnsemblBacteria" id="CAI50754">
        <property type="protein sequence ID" value="CAI50754"/>
        <property type="gene ID" value="NP_5326A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702333"/>
    <dbReference type="KEGG" id="nph:NP_5326A"/>
    <dbReference type="eggNOG" id="arCOG01009">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_197235_1_0_2"/>
    <dbReference type="OrthoDB" id="56938at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22231">
        <property type="entry name" value="RHH_NikR_HicB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="entry name" value="Met repressor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002145">
        <property type="entry name" value="CopG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36215">
        <property type="entry name" value="BLL4998 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36215:SF1">
        <property type="entry name" value="BLL4998 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01402">
        <property type="entry name" value="RHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="Ribbon-helix-helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribbon-helix-helix protein CopG" evidence="1">
        <location>
            <begin position="2"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="57" mass="6800" checksum="7190FB00ADDAE1F1" modified="2017-05-10" version="1">MERVTLRIPKQQIEEVERMVDTGQFPNRSEAIRSAVREMINEQETESQEQNRPWAKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZN8</accession>
    <name>A0A1U7EZN8_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1611 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_5374A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324386.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZN8"/>
    <dbReference type="STRING" id="348780.NP_5374A"/>
    <dbReference type="EnsemblBacteria" id="CAI50778">
        <property type="protein sequence ID" value="CAI50778"/>
        <property type="gene ID" value="NP_5374A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702356"/>
    <dbReference type="KEGG" id="nph:NP_5374A"/>
    <dbReference type="eggNOG" id="arCOG14727">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062231_0_0_2"/>
    <dbReference type="OrthoDB" id="350542at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="335" mass="35533" checksum="DE31C2F2D556137D" modified="2017-05-10" version="1">MKANIHTAVTRIADLEGRAYEIEPRPRNRWGRGDYVLATVTRRPDVDVRVENPHGRRVETMEGETIVGALGTRRATRGVVGSWRDVDDSGAMHVMTGGGVLGRVTSASPFAPSPIDIEYEGHVVIDGDIARMDDYGLSAASPALDTPVVLVLGTSMSSGKTMSGRVVTRVLVEEGYDVAACKLTGAGTYDDIQSLGVAGADPACDFVDGGLPTTIVPEDRFRRAAGGVLERMQDADVVVAEVGASPLEPYNGAAAVDLVEDQVAFTLLCATDPYAVVGIEEAFGWSPDLVTGITTNTTAGIELVEQLADCPALNLLKEETKPHMRDLLVEALPTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILV8</accession>
    <name>Q3ILV8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">FNT family transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">tp47</name>
        <name evidence="6" type="ordered locus">NP_6264A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6 7">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324509.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILV8"/>
    <dbReference type="EnsemblBacteria" id="CAI50912">
        <property type="protein sequence ID" value="CAI50912"/>
        <property type="gene ID" value="NP_6264A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694609"/>
    <dbReference type="KEGG" id="nph:NP_6264A"/>
    <dbReference type="HOGENOM" id="CLU_061519_0_0_2"/>
    <dbReference type="OrthoDB" id="195948at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015499">
        <property type="term" value="F:formate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000292">
        <property type="entry name" value="For/NO2_transpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30520">
        <property type="entry name" value="FORMATE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30520:SF2">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YFDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01226">
        <property type="entry name" value="Form_Nir_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="189"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="231"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI50912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="295" mass="31947" checksum="C2FD3584E3BC2256" modified="2005-11-08" version="1">MGNGNETIFRNRFSTDEIYERVVVEADEEMNRSMPELFFSALAGGFAISITFLLYVSGTHAADGAAILGAILYPLGFIYIIIGNYQLYTENTLPPVALVLERIASLPAMLRMYGIVLWGNLAGGTIGALVLAHGGVFDAATTQTAIEIATSGVETNWWPLFFKAMFAGLIVAGVVWMDFSVSNAMARVTLIYMAFLAIPVGGLFHIVVASTEVMFLVFLGEIGLWVGAVQFALPVLLGNTLGGILLVTIVNYFQTSDKVHGFEGQLPLKEWVLTVNTGSIEADALLAELERRIDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM27</accession>
    <name>Q3IM27_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_6116A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM27"/>
    <dbReference type="EnsemblBacteria" id="CAI50838">
        <property type="protein sequence ID" value="CAI50838"/>
        <property type="gene ID" value="NP_6116A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_6116A"/>
    <dbReference type="HOGENOM" id="CLU_2712950_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="72" mass="8119" checksum="C322E278D8EDFC4F" modified="2005-11-08" version="1">MRERPPEPSNGRERLQHRPVRGVSTDEGCSHPFDVVRFPVVFGNKHASVQSRDPVLRVLDRSLIQYAPSVGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM39</accession>
    <name>Q3IM39_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Arsenate reductase (Glutaredoxin)</fullName>
            <ecNumber evidence="3">1.20.4.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">arsC</name>
        <name evidence="3" type="ordered locus">NP_6092A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.20.4.1" evidence="3"/>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324428.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM39"/>
    <dbReference type="EnsemblBacteria" id="CAI50826">
        <property type="protein sequence ID" value="CAI50826"/>
        <property type="gene ID" value="NP_6092A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694681"/>
    <dbReference type="KEGG" id="nph:NP_6092A"/>
    <dbReference type="HOGENOM" id="CLU_071415_3_3_2"/>
    <dbReference type="OrthoDB" id="295776at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008794">
        <property type="term" value="F:arsenate reductase (glutaredoxin) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046685">
        <property type="term" value="P:response to arsenic-containing substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023485">
        <property type="entry name" value="Ptyr_pPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036196">
        <property type="entry name" value="Ptyr_pPase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43428">
        <property type="entry name" value="ARSENATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43428:SF1">
        <property type="entry name" value="ARSENATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01451">
        <property type="entry name" value="LMWPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00226">
        <property type="entry name" value="LMWPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52788">
        <property type="entry name" value="Phosphotyrosine protein phosphatases I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0059">Arsenical resistance</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphotyrosine protein phosphatase I" evidence="2">
        <location>
            <begin position="5"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="137" mass="15022" checksum="F607E966DADB7719" modified="2005-11-08" version="1">MTETTRIGFVCVQSAGRSQMATAFAEQERNKRGLEAEVDIVTGGTAPAESVHNIVIRVMEEVDIDIAERSPQKITPAELEQCDVVATMGCSTLELETTVDVRDWALADPDDATVEEARAIRDAVRSNVQALFDELQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM54</accession>
    <name>Q3IM54_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ArsR family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_6056A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5 6">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324413.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM54"/>
    <dbReference type="EnsemblBacteria" id="CAI50808">
        <property type="protein sequence ID" value="CAI50808"/>
        <property type="gene ID" value="NP_6056A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694695"/>
    <dbReference type="KEGG" id="nph:NP_6056A"/>
    <dbReference type="HOGENOM" id="CLU_097806_3_0_2"/>
    <dbReference type="OrthoDB" id="46231at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051011">
        <property type="entry name" value="Metal_resp_trans_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033788">
        <property type="entry name" value="HTH_metalloreg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43132">
        <property type="entry name" value="ARSENICAL RESISTANCE OPERON REPRESSOR ARSR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43132:SF2">
        <property type="entry name" value="ARSENICAL RESISTANCE OPERON REPRESSOR ARSR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="4">
        <location>
            <begin position="38"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI50808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="133" mass="14573" checksum="E6F5963FE6B45B64" modified="2005-11-08" version="1">MAQTTERLQRYLSDELGECCSEDVEQRLNELETLGDALATEQIASELDVLSALANETRYTIIRVLVAAEEELCVCELNAVVDVTESGLSHALSQLVDADLVDGRKDGRWKKYRATNRAVALVTLLEGSVSTDA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMX7</accession>
    <name>RL24_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL24</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">50S ribosomal protein L24</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl24</name>
        <name type="ordered locus">NP_4876A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of two assembly initiator proteins, it binds directly to the 5'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Located at the polypeptide exit tunnel on the outside of the subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL24 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324141.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMX7"/>
    <dbReference type="SMR" id="Q3IMX7"/>
    <dbReference type="STRING" id="348780.NP_4876A"/>
    <dbReference type="EnsemblBacteria" id="CAI50529">
        <property type="protein sequence ID" value="CAI50529"/>
        <property type="gene ID" value="NP_4876A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703134"/>
    <dbReference type="KEGG" id="nph:NP_4876A"/>
    <dbReference type="eggNOG" id="arCOG04094">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093240_2_1_2"/>
    <dbReference type="OrthoDB" id="10899at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06089">
        <property type="entry name" value="KOW_RPL26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01326_A">
        <property type="entry name" value="Ribosomal_uL24_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005756">
        <property type="entry name" value="Ribosomal_uL24_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041988">
        <property type="entry name" value="Ribosomal_uL24_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01080">
        <property type="entry name" value="rplX_A_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11143">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L26 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16906">
        <property type="entry name" value="Ribosomal_L26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000241698" description="Large ribosomal subunit protein uL24">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="10"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="120" mass="13671" checksum="7DFC14271758E4E3" modified="2005-11-08" version="1">MTRQPHKQRNRQERAALHEKQKQVRAPLSPELREEYGQRNVRVNAGDTVEVQRGDFAGESGEVVTVDLRAAEIHVEDVTQETADGEEVPRPLEASNVQVTELDLEDEVREARLESEEDNE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q3INZ0</accession>
    <name>GCH3_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GTP cyclohydrolase III</fullName>
            <ecNumber evidence="1">3.5.4.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gch3</name>
        <name type="ordered locus">NP_4142A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of 2-amino-5-formylamino-6-ribofuranosylamino-4(3H)-pyrimidinone ribonucleotide monophosphate and inorganic phosphate from GTP. Also has an independent pyrophosphate phosphohydrolase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP + 3 H2O = 2-amino-5-formylamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + 2 phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:22468"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57258"/>
            <dbReference type="EC" id="3.5.4.29"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal-type GTP cyclohydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.29" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323778.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3INZ0"/>
    <dbReference type="SMR" id="Q3INZ0"/>
    <dbReference type="STRING" id="348780.NP_4142A"/>
    <dbReference type="EnsemblBacteria" id="CAI50162">
        <property type="protein sequence ID" value="CAI50162"/>
        <property type="gene ID" value="NP_4142A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702742"/>
    <dbReference type="KEGG" id="nph:NP_4142A"/>
    <dbReference type="eggNOG" id="arCOG04202">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080076_0_0_2"/>
    <dbReference type="OrthoDB" id="25211at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043740">
        <property type="term" value="F:GTP cyclohydrolase IIa activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00608">
        <property type="entry name" value="GTP_cyclohydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007839">
        <property type="entry name" value="GTP_CycHdrlase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002587">
        <property type="entry name" value="PRK02240.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42202">
        <property type="entry name" value="GTP CYCLOHYDROLASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42202:SF1">
        <property type="entry name" value="GTP CYCLOHYDROLASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05165">
        <property type="entry name" value="GCH_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009265">
        <property type="entry name" value="GTP_cyclohydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000056696" description="GTP cyclohydrolase III">
        <location>
            <begin position="1"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00608"/>
        </source>
    </evidence>
    <sequence length="253" mass="27758" checksum="43FCD05ADB54476C" modified="2005-11-08" version="1">MTNTQVTLVQIDNYGPWTVTPEPRREVDLQTLQSRLYADLSQLIGNRMGYVFFTRFDNMVAVTNGLDADAHALIQESVGNRYPVTVSLSIGVDSSPAAALGTATDQLQDAGSAQDKGRTEILRGDPIQPSERTDTDVQIAHFDVNDATGKYTDQLNEFDSFINIEQGYAELMRYMRHENDSLSFFVGGDNIIAVCDGIDEAAYLDAIEHVNETVGVELKVGVGLDRTAQAAGMAAKHALETCREENTDVEFAR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IPJ7</accession>
    <name>RNP3_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease P protein component 3</fullName>
            <shortName evidence="1">RNase P component 3</shortName>
            <ecNumber evidence="1">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Rpp30</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rnp3</name>
        <name type="ordered locus">NP_3726A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Consists of a catalytic RNA component and at least 4-5 protein subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic/archaeal RNase P protein component 3 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323571.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IPJ7"/>
    <dbReference type="SMR" id="Q3IPJ7"/>
    <dbReference type="STRING" id="348780.NP_3726A"/>
    <dbReference type="EnsemblBacteria" id="CAI49954">
        <property type="protein sequence ID" value="CAI49954"/>
        <property type="gene ID" value="NP_3726A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703114"/>
    <dbReference type="KEGG" id="nph:NP_3726A"/>
    <dbReference type="eggNOG" id="arCOG00307">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074509_1_0_2"/>
    <dbReference type="OrthoDB" id="85765at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00756">
        <property type="entry name" value="RNase_P_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023539">
        <property type="entry name" value="RNase_P_comp-3_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002738">
        <property type="entry name" value="RNase_P_p30"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01876">
        <property type="entry name" value="RNase_P_p30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="PHP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_1000046630" description="Ribonuclease P protein component 3">
        <location>
            <begin position="1"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00756"/>
        </source>
    </evidence>
    <sequence length="236" mass="25695" checksum="CA7EFF4594397539" modified="2005-11-08" version="1">MYEGVHAHPDGNATVARFAETAASRGYDGIVVRNHGDAQTDYDADAVAAEYGVEVVSGIEIRTDDTAQASGLVGNYRSKRDIVCVHGGELNRFAVEEPKVDVLAHPMRDGDVNHVLAKAAAENGVHFEFNFGRVLRADGGKRVQAIQGLRKLRELVDQYDAPYVVSADPESHLELRSSRDLAAVGETVGFDREAITDGLAAWADIVERNRRRRSAAVVEPGVRIERADGETDDSRE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-11-25" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IQZ9</accession>
    <name>KATG_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Catalase-peroxidase</fullName>
            <shortName evidence="1">CP</shortName>
            <ecNumber evidence="1">1.11.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Peroxidase/catalase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">katG</name>
        <name type="ordered locus">NP_2708A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O2 + AH2 = A + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:30275"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="EC" id="1.11.1.21"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 H2O2 = O2 + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:20309"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="EC" id="1.11.1.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="1">Binds 1 heme b (iron(II)-protoporphyrin IX) group per dimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer or homotetramer.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Formation of the three residue Trp-Tyr-Met cross-link is important for the catalase, but not the peroxidase activity of the enzyme.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peroxidase family. Peroxidase/catalase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.21" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323070.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IQZ9"/>
    <dbReference type="SMR" id="Q3IQZ9"/>
    <dbReference type="STRING" id="348780.NP_2708A"/>
    <dbReference type="PeroxiBase" id="2515">
        <property type="entry name" value="NpCP01"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI49445">
        <property type="protein sequence ID" value="CAI49445"/>
        <property type="gene ID" value="NP_2708A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703016"/>
    <dbReference type="KEGG" id="nph:NP_2708A"/>
    <dbReference type="eggNOG" id="arCOG04487">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025424_2_0_2"/>
    <dbReference type="OrthoDB" id="358790at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004096">
        <property type="term" value="F:catalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070301">
        <property type="term" value="P:cellular response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08200">
        <property type="entry name" value="catalase_peroxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000004">
        <property type="entry name" value="Catalase-peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.10:FF:000002">
        <property type="entry name" value="Catalase-peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01961">
        <property type="entry name" value="Catal_peroxid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000763">
        <property type="entry name" value="Catalase_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00198">
        <property type="entry name" value="cat_per_HPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011635">
        <property type="entry name" value="PRK15061.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30555:SF0">
        <property type="entry name" value="CATALASE-PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30555">
        <property type="entry name" value="HYDROPEROXIDASE I, BIFUNCTIONAL CATALASE-PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00460">
        <property type="entry name" value="BPEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0349">Heme</keyword>
    <keyword id="KW-0376">Hydrogen peroxide</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0575">Peroxidase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000354970" description="Catalase-peroxidase">
        <location>
            <begin position="1"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="1">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="74"/>
        </location>
    </feature>
    <feature type="cross-link" description="Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-226)" evidence="1">
        <location>
            <begin position="77"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="cross-link" description="Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-77)" evidence="1">
        <location>
            <begin position="200"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01961"/>
        </source>
    </evidence>
    <sequence length="713" mass="79455" checksum="90FA3F659414A865" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IRE7</accession>
    <name>SYT_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Threonine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Threonyl-tRNA synthetase</fullName>
            <shortName evidence="1">ThrRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thrS</name>
        <name type="ordered locus">NP_2410A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Thr) + L-threonine + ATP = L-threonyl-tRNA(Thr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24624"/>
            <dbReference type="Rhea" id="RHEA-COMP:9670"/>
            <dbReference type="Rhea" id="RHEA-COMP:9704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78534"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.3" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322922.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IRE7"/>
    <dbReference type="SMR" id="Q3IRE7"/>
    <dbReference type="STRING" id="348780.NP_2410A"/>
    <dbReference type="EnsemblBacteria" id="CAI49296">
        <property type="protein sequence ID" value="CAI49296"/>
        <property type="gene ID" value="NP_2410A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703256"/>
    <dbReference type="KEGG" id="nph:NP_2410A"/>
    <dbReference type="eggNOG" id="arCOG00401">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008554_0_1_2"/>
    <dbReference type="OrthoDB" id="372136at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004829">
        <property type="term" value="F:threonine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006435">
        <property type="term" value="P:threonyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01667">
        <property type="entry name" value="TGS_ThrRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00860">
        <property type="entry name" value="ThrRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00771">
        <property type="entry name" value="ThrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000002">
        <property type="entry name" value="Threonine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000001">
        <property type="entry name" value="Threonine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.980.10:FF:000005">
        <property type="entry name" value="Threonyl-tRNA synthetase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.54.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00184">
        <property type="entry name" value="Thr_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002320">
        <property type="entry name" value="Thr-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047246">
        <property type="entry name" value="ThrRS_anticodon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033728">
        <property type="entry name" value="ThrRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00418">
        <property type="entry name" value="thrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451:SF44">
        <property type="entry name" value="THREONINE--TRNA LIGASE, CHLOROPLASTIC_MITOCHONDRIAL 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451">
        <property type="entry name" value="THREONINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01047">
        <property type="entry name" value="TRNASYNTHTHR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_1000020448" description="Threonine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="2">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Catalytic" evidence="1">
        <location>
            <begin position="242"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="385"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="510"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01228"/>
        </source>
    </evidence>
    <sequence length="642" mass="73507" checksum="4A24FB50D1419BE0" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IRX3</accession>
    <name>FOLD_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Bifunctional protein FolD</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="1">Methylenetetrahydrofolate dehydrogenase</fullName>
                <ecNumber evidence="1">1.5.1.5</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="1">Methenyltetrahydrofolate cyclohydrolase</fullName>
                <ecNumber evidence="1">3.5.4.9</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="1" type="primary">folD</name>
        <name type="ordered locus">NP_2054A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADP(+) = (6R)-5,10-methenyltetrahydrofolate + NADPH</text>
            <dbReference type="Rhea" id="RHEA:22812"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-5,10-methenyltetrahydrofolate + H2O = (6R)-10-formyltetrahydrofolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="3.5.4.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.5" evidence="1"/>
    <dbReference type="EC" id="3.5.4.9" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322747.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IRX3"/>
    <dbReference type="SMR" id="Q3IRX3"/>
    <dbReference type="STRING" id="348780.NP_2054A"/>
    <dbReference type="EnsemblBacteria" id="CAI49118">
        <property type="protein sequence ID" value="CAI49118"/>
        <property type="gene ID" value="NP_2054A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702724"/>
    <dbReference type="KEGG" id="nph:NP_2054A"/>
    <dbReference type="eggNOG" id="arCOG04538">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034045_1_2_2"/>
    <dbReference type="OrthoDB" id="9455at2157"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004477">
        <property type="term" value="F:methenyltetrahydrofolate cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004488">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01080">
        <property type="entry name" value="NAD_bind_m-THF_DH_Cyclohyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000189">
        <property type="entry name" value="Bifunctional protein FolD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000005">
        <property type="entry name" value="C-1-tetrahydrofolate synthase, cytoplasmic, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01576">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000672">
        <property type="entry name" value="THF_DH/CycHdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020630">
        <property type="entry name" value="THF_DH/CycHdrlase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020631">
        <property type="entry name" value="THF_DH/CycHdrlase_NAD-bd_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010764">
        <property type="entry name" value="PRK14167.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099:SF5">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00763">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02882">
        <property type="entry name" value="THF_DHG_CYH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00085">
        <property type="entry name" value="THFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0486">Methionine biosynthesis</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000268587" description="Bifunctional protein FolD">
        <location>
            <begin position="1"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="164"/>
            <end position="166"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01576"/>
        </source>
    </evidence>
    <sequence length="297" mass="31496" checksum="9B44496987B22824" modified="2005-11-08" version="1">MTEVIDGNAVASDIRDDLTDAIATLADAGARPGLATVLMGDDPASETYVNMKQRDCEEVGIESYHVDVDGDAPPETLYDEIAALNQNDDVHGYIVQAPVPDHVDYREVIRRVDPQKDVDGFHPENVGRLVAGDARFRPCTPHGVQKLLEAADIDTEGKDVTIVGRSDIVGKPLANLLIQKADDGNATVTVCHSRTENLAAKTRRADIVVAAAGAPELVDGSMIGEGSVVIDVGVNRVDADNEKGYELVGDVEYESATQNASAITPVPGGVGPMTRAMLLYNTVKAASLQEDIDVALP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-01-23" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IS07</accession>
    <name>PSTB1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphate import ATP-binding protein PstB 1</fullName>
            <ecNumber evidence="1">7.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ABC phosphate transporter 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphate-transporting ATPase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pstB1</name>
        <name type="synonym">abc14a</name>
        <name type="ordered locus">NP_1984A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the ABC transporter complex PstSACB involved in phosphate import. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>phosphate(out) + ATP + H2O = ADP + 2 phosphate(in) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24440"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.3.2.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The complex is composed of two ATP-binding proteins (PstB), two transmembrane proteins (PstC and PstA) and a solute-binding protein (PstS).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. Phosphate importer (TC 3.A.1.7) family.</text>
    </comment>
    <dbReference type="EC" id="7.3.2.1" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322713.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IS07"/>
    <dbReference type="SMR" id="Q3IS07"/>
    <dbReference type="STRING" id="348780.NP_1984A"/>
    <dbReference type="EnsemblBacteria" id="CAI49083">
        <property type="protein sequence ID" value="CAI49083"/>
        <property type="gene ID" value="NP_1984A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702411"/>
    <dbReference type="KEGG" id="nph:NP_1984A"/>
    <dbReference type="eggNOG" id="arCOG00231">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="OrthoDB" id="31298at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015415">
        <property type="term" value="F:ATPase-coupled phosphate ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03260">
        <property type="entry name" value="ABC_PstB_phosphate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005670">
        <property type="entry name" value="PstB-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00972">
        <property type="entry name" value="3a0107s01c2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43423">
        <property type="entry name" value="ABC TRANSPORTER I FAMILY MEMBER 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43423:SF1">
        <property type="entry name" value="ABC TRANSPORTER I FAMILY MEMBER 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51238">
        <property type="entry name" value="PSTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0592">Phosphate transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000272591" description="Phosphate import ATP-binding protein PstB 1">
        <location>
            <begin position="1"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="1">
        <location>
            <begin position="38"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="70"/>
            <end position="77"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="283" mass="31004" checksum="D6188FEDFD920365" modified="2005-11-08" version="1">MSSDDTTDPTADDESFTDSPVAGLEQSTTTRGSGRTVISARNINVWYNETQALSDITMDIPEQEVTALIGPSGCGKSTFLRCINRMNDRIDACRIEGELELHGKNVYDEDVDPVALRRKVGMVFQKPNPFPKSIYDNVAYGLKIQGYDGDIDARVEEALRGAALWDEVNDQLDESGLELSGGQQQRLCIARAIAPDPEVVLMDEPTSALDPVAASKVEDLIDELVEDYTVVIVTHNMQQAARISDKTAVFLTGGELVEFDDTASVFENPSSERVENYITGKFG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IT10</accession>
    <name>HIS4_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase</fullName>
            <ecNumber evidence="1">5.3.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisA</name>
        <name type="ordered locus">NP_1268A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide = 5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:15469"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="5.3.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 4/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HisA/HisF family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.16" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322360.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IT10"/>
    <dbReference type="SMR" id="Q3IT10"/>
    <dbReference type="STRING" id="348780.NP_1268A"/>
    <dbReference type="EnsemblBacteria" id="CAI48725">
        <property type="protein sequence ID" value="CAI48725"/>
        <property type="gene ID" value="NP_1268A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702814"/>
    <dbReference type="KEGG" id="nph:NP_1268A"/>
    <dbReference type="eggNOG" id="arCOG00618">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048577_1_1_2"/>
    <dbReference type="OrthoDB" id="52866at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00009"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003949">
        <property type="term" value="F:1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04732">
        <property type="entry name" value="HisA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000009">
        <property type="entry name" value="1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01014">
        <property type="entry name" value="HisA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023016">
        <property type="entry name" value="HisA/PriA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006063">
        <property type="entry name" value="HisA_bact_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044524">
        <property type="entry name" value="Isoase_HisA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00007">
        <property type="entry name" value="1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010112">
        <property type="entry name" value="PRK13585.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090">
        <property type="entry name" value="1-(5-PHOSPHORIBOSYL)-5-[(5-PHOSPHORIBOSYLAMINO)METHYLIDENEAMINO] IMIDAZOLE-4-CARBOXAMIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090:SF7">
        <property type="entry name" value="1-(5-PHOSPHORIBOSYL)-5-[(5-PHOSPHORIBOSYLAMINO)METHYLIDENEAMINO] IMIDAZOLE-4-CARBOXAMIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000229100" description="1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase">
        <location>
            <begin position="1"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01014"/>
        </source>
    </evidence>
    <sequence length="239" mass="24424" checksum="E26DC8B142067865" modified="2005-11-08" version="1">MFPSFEVIPAVDMQDGQVVQLVGGERGTETEYGDPVAAAQRWVDAGAETLHLVDLDGAFEGERANADAVEAVLEATDVSVQLGGGIRTVDDADSLLSMGVDRVILGTAAVENPDIVGEINDRHPGSVVVSLDAKDGEVVVSGWTESTGLDPAEAAARYEAEGAGGVLFTDVDVEGQLSGVRADEIARVVDAVDIPVIASGGVSTLSDIEALKDAGAAATVVGTALYEGEFTLEEAAAVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IUF9</accession>
    <name>Q3IUF9_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Type II methyltransferase</fullName>
            <ecNumber evidence="8">2.1.1.113</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">N-4 cytosine-specific methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">zim</name>
        <name evidence="10" type="ordered locus">NP_0260A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = an N(4)-methyl-2'-deoxycytidine in DNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16857"/>
            <dbReference type="Rhea" id="RHEA-COMP:11369"/>
            <dbReference type="Rhea" id="RHEA-COMP:13674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:85452"/>
            <dbReference type="ChEBI" id="CHEBI:137933"/>
            <dbReference type="EC" id="2.1.1.113"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.113" evidence="8"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321860.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IUF9"/>
    <dbReference type="STRING" id="348780.NP_0260A"/>
    <dbReference type="REBASE" id="11438">
        <property type="entry name" value="M.NphDORF260P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI48221">
        <property type="protein sequence ID" value="CAI48221"/>
        <property type="gene ID" value="NP_0260A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700806"/>
    <dbReference type="KEGG" id="nph:NP_0260A"/>
    <dbReference type="eggNOG" id="arCOG00115">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024927_2_2_2"/>
    <dbReference type="OrthoDB" id="38200at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015667">
        <property type="term" value="F:site-specific DNA-methyltransferase (cytosine-N4-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002941">
        <property type="entry name" value="DNA_methylase_N4/N6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017985">
        <property type="entry name" value="MeTrfase_CN4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001091">
        <property type="entry name" value="RM_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01555">
        <property type="entry name" value="N6_N4_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00508">
        <property type="entry name" value="S21N4MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00093">
        <property type="entry name" value="N4_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 8 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0680">Restriction system</keyword>
    <keyword evidence="4 8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA methylase N-4/N-6" evidence="9">
        <location>
            <begin position="23"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI48221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="346" mass="39244" checksum="A022C1738A767297" modified="2005-11-08" version="1">MKTTHRIVAGDARGLEELDDDAVELVVTSPPYPMVELWDDLFADLDGTVASLLSDGDGDAAFEAMHEQLDAVWRELSRVLVDGGIACVNVGDATRTLGDGFRVYQNHARIIEAFESLGFDPLPEVLWRKPANSTAKFMGSGMVPPNAYVTLEHEYILVFRNGGRRAFEPNADRRYEAAYFWEERNQWFSDVWTDVRGETQTLDNEDLRDRSAAYPFEIPYRLINMYSTYGDTVLDPFWGTGTTTLAAMVAGRNSVGYELDDGLVEAFDDRAADVPALSRRVTDERLDEHRAFIDRRRSTGESFEYRADNYDFPVRTKQERGIQLYTAEAVTETDSGYRVRHEPKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IUQ8</accession>
    <name>Q3IUQ8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Metalloprotease JAMM1, desampylating</fullName>
            <ecNumber evidence="7">3.4.24.88</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">jamm1</name>
        <name evidence="7" type="ordered locus">NP_0062A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.4.24.88" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321761.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IUQ8"/>
    <dbReference type="STRING" id="348780.NP_0062A"/>
    <dbReference type="MEROPS" id="M67.012"/>
    <dbReference type="EnsemblBacteria" id="CAI48122">
        <property type="protein sequence ID" value="CAI48122"/>
        <property type="gene ID" value="NP_0062A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700749"/>
    <dbReference type="KEGG" id="nph:NP_0062A"/>
    <dbReference type="eggNOG" id="arCOG01138">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116765_1_0_2"/>
    <dbReference type="OrthoDB" id="10589at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08070">
        <property type="entry name" value="MPN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000085">
        <property type="entry name" value="Mov34/MPN/PAD-1 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028090">
        <property type="entry name" value="JAB_dom_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053551">
        <property type="entry name" value="Metalloprotease_DSAMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051929">
        <property type="entry name" value="VirAsm_ModProt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041370">
        <property type="entry name" value="desamp_Halo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34858">
        <property type="entry name" value="CYSO-CYSTEINE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34858:SF1">
        <property type="entry name" value="CYSO-CYSTEINE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14464">
        <property type="entry name" value="Prok-JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102712">
        <property type="entry name" value="JAB1/MPN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MPN" evidence="6">
        <location>
            <begin position="2"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="137" mass="14888" checksum="098FBDE76915FB5A" modified="2005-11-08" version="1">MTLRLPAAVRDAIVAHARAGTPEEVVGALAGHHGEDCSTVERAYRARNAAERPRTRYEIAPTQELSLLDSIDTAGLDVVGFYHSHPDGPGEPSRTDAELAAWPGYSYLIVSLAGEEPVVGCWRWTGETFRSEDTQVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E1H8A7</accession>
    <name>A0A1E1H8A7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribbon-helix-helix protein CopG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4632A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324021.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E1H8A7"/>
    <dbReference type="STRING" id="348780.NP_4632A"/>
    <dbReference type="EnsemblBacteria" id="CAI50407">
        <property type="protein sequence ID" value="CAI50407"/>
        <property type="gene ID" value="NP_4632A"/>
    </dbReference>
    <dbReference type="GeneID" id="32155022"/>
    <dbReference type="KEGG" id="nph:NP_4632A"/>
    <dbReference type="OrthoDB" id="333878at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="entry name" value="Met repressor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002145">
        <property type="entry name" value="CopG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01402">
        <property type="entry name" value="RHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="Ribbon-helix-helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribbon-helix-helix protein CopG" evidence="1">
        <location>
            <begin position="3"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="44" mass="5362" checksum="976D36B97DB7808D" modified="2017-01-18" version="1">MTKRLTVDFEDDLYKEFSKKCIDVERPKSEVVRELVQDWVNDQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-04-12" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1Q2UAX5</accession>
    <name>A0A1Q2UAX5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Oxoglutarate--ferredoxin oxidoreductase beta subunit</fullName>
            <ecNumber evidence="14">1.2.7.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">korB2</name>
        <name evidence="13" type="synonym">korB1</name>
        <name evidence="13" type="ordered locus">NP_1234A</name>
        <name evidence="14" type="ordered locus">NP_3012A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
            <strain evidence="14">Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="1.2.7.3" evidence="14"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322344.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1Q2UAX5"/>
    <dbReference type="STRING" id="348780.NP_1234A"/>
    <dbReference type="EnsemblBacteria" id="CAI48708">
        <property type="protein sequence ID" value="CAI48708"/>
        <property type="gene ID" value="NP_1234A"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI49597">
        <property type="protein sequence ID" value="CAI49597"/>
        <property type="gene ID" value="NP_3012A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702874"/>
    <dbReference type="KEGG" id="nph:NP_1234A"/>
    <dbReference type="KEGG" id="nph:NP_3012A"/>
    <dbReference type="eggNOG" id="arCOG01599">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048564_0_0_2"/>
    <dbReference type="OrthoDB" id="30755at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045333">
        <property type="term" value="P:cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03375">
        <property type="entry name" value="TPP_OGFOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051457">
        <property type="entry name" value="2-oxoacid:Fd_oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011896">
        <property type="entry name" value="OFOB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032686">
        <property type="entry name" value="PFO_beta_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02177">
        <property type="entry name" value="PorB_KorB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48084:SF4">
        <property type="entry name" value="2-OXOGLUTARATE OXIDOREDUCTASE SUBUNIT KORB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48084">
        <property type="entry name" value="2-OXOGLUTARATE OXIDOREDUCTASE SUBUNIT KORB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12367">
        <property type="entry name" value="PFO_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="11">
        <location>
            <begin position="51"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate ferredoxin oxidoreductase beta subunit C-terminal" evidence="12">
        <location>
            <begin position="202"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="130"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="143"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF12367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAI48708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAI49597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="289" mass="31444" checksum="72E1F5F11B4C8AC6" modified="2017-04-12" version="1">MSSNVRFTDFKSDKQPTWCPGCGDFGTMNGMMKALAETGNDPDNTFVVAGIGCSGKIGTYMHSYALHGVHGRALPVGTGVKMANPELEVMVAGGDGDGYSIGAGHFIHAVRRNVDMTYVVMDNRIYGLTKGQASPTSREDFETSTSPDGTNQQPVNPLALALAAGGTFIAQSFSSDSQRHAELVQEAIEHDGFGFVNVYSPCVTFNDVDTYDYFRDAIVDVGDEDVDHDPSDYDEAKELILDREKEYQGVLYQDESSVPFEVREGVDEPMVDIPDGAPEDATDLVREFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ET89</accession>
    <name>A0A1U7ET89_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Hsp20-type molecular chaperone</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hsp20A</name>
        <name evidence="4" type="ordered locus">NP_0010A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321735.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ET89"/>
    <dbReference type="STRING" id="348780.NP_0010A"/>
    <dbReference type="EnsemblBacteria" id="CAI48096">
        <property type="protein sequence ID" value="CAI48096"/>
        <property type="gene ID" value="NP_0010A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702829"/>
    <dbReference type="KEGG" id="nph:NP_0010A"/>
    <dbReference type="eggNOG" id="arCOG01832">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046737_8_8_2"/>
    <dbReference type="OrthoDB" id="26084at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06464">
        <property type="entry name" value="ACD_sHsps-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SHSP" evidence="3">
        <location>
            <begin position="32"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="124" mass="13728" checksum="3068A78B46F9254A" modified="2017-05-10" version="1">MRRDDRDDPFDDIFDEIERMMNQMTGGIDAGDAGFGGDAHVSVYEDDDEIRVVADLPGVDKDDLTIQCDGRRVTIAATTDHSDYKERIELPARVDEHSASATFNNGVLEVEFERTGSSADIDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ET94</accession>
    <name>A0A1U7ET94_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF7382 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_0022A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321741.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ET94"/>
    <dbReference type="STRING" id="348780.NP_0022A"/>
    <dbReference type="EnsemblBacteria" id="CAI48102">
        <property type="protein sequence ID" value="CAI48102"/>
        <property type="gene ID" value="NP_0022A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700740"/>
    <dbReference type="KEGG" id="nph:NP_0022A"/>
    <dbReference type="eggNOG" id="arCOG02981">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1745500_0_0_2"/>
    <dbReference type="OrthoDB" id="300879at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055806">
        <property type="entry name" value="DUF7382"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24107">
        <property type="entry name" value="DUF7382"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7382" evidence="3">
        <location>
            <begin position="45"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="149" mass="15842" checksum="02591599E9CBCD6B" modified="2017-05-10" version="1">MRQLRRLWADNRGIEGLPVRLVIALVVGVASLSVMMNMLSGISGLAVTELDVRPDPEVVTPGDHTVDIEVVDPDGNSVADATVVARGGSARLDGVETATTDADGHTQLDLSPTLRPNQRDGTVEFDIKPPAGSEYADERENTALLVVDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETB1</accession>
    <name>A0A1U7ETB1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NH(3)-dependent NAD(+) synthetase</fullName>
            <ecNumber evidence="7">6.3.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">nadE2</name>
        <name evidence="9" type="ordered locus">NP_0054A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>deamido-NAD(+) + NH4(+) + ATP = AMP + diphosphate + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21188"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; NAD(+) biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NAD synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.5" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETB1"/>
    <dbReference type="STRING" id="348780.NP_0054A"/>
    <dbReference type="EnsemblBacteria" id="CAI48118">
        <property type="protein sequence ID" value="CAI48118"/>
        <property type="gene ID" value="NP_0054A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_0054A"/>
    <dbReference type="eggNOG" id="arCOG00069">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059327_1_1_2"/>
    <dbReference type="OrthoDB" id="39312at2157"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00333"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003952">
        <property type="term" value="F:NAD+ synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008795">
        <property type="term" value="F:NAD+ synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00553">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003694">
        <property type="entry name" value="NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00552">
        <property type="entry name" value="nadE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010587">
        <property type="entry name" value="PRK13980.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090:SF9">
        <property type="entry name" value="GLUTAMINE-DEPENDENT NAD(+) SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090">
        <property type="entry name" value="NH 3 /GLUTAMINE-DEPENDENT NAD + SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 6 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 6" id="KW-0520">NAD</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD/GMP synthase" evidence="8">
        <location>
            <begin position="20"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI48118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="270" mass="28678" checksum="EDFACAD577B90BBE" modified="2017-05-10" version="1">MTDALPPGVSFRADTEAMAAEAVDCIETTVDAADADGVVVGLSGGLDSTTTATLAVEALGSERVYGLVLPSSKLGSKSAQDAETVADVLGIDHETIHLQPLLACFGDMVPGPVDLHGDPVVRGNLVARLRMSMLYLTANAMERLVVGTTNRSEHLLGYFTKHGDGAADVLPLAHLYKTEVERLADALEVPPFIAEKPPTAGFYPGQSDRADFGAPYTTVDAVLKRLVADDRTTDAICSELGVDAAVVDRVKRHHEQTAHKRRRPPCPSVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETL3</accession>
    <name>A0A1U7ETL3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Kef-type transport system</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">kef2</name>
        <name evidence="11" type="synonym">tp40a</name>
        <name evidence="11" type="ordered locus">NP_0330A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321894.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETL3"/>
    <dbReference type="STRING" id="348780.NP_0330A"/>
    <dbReference type="EnsemblBacteria" id="CAI48256">
        <property type="protein sequence ID" value="CAI48256"/>
        <property type="gene ID" value="NP_0330A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703316"/>
    <dbReference type="KEGG" id="nph:NP_0330A"/>
    <dbReference type="eggNOG" id="arCOG01955">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005126_9_0_2"/>
    <dbReference type="OrthoDB" id="43518at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42751:SF6">
        <property type="entry name" value="CONSERVED INTEGRAL MEMBRANE TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42751">
        <property type="entry name" value="SODIUM/HYDROGEN EXCHANGER FAMILY/TRKA DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="59"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="97"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="226"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="255"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="285"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="316"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="349"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="374"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="9">
        <location>
            <begin position="16"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="10">
        <location>
            <begin position="432"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="574"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="574"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI48256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="593" mass="64640" checksum="4B6EEE09CC47D0AE" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETN1</accession>
    <name>A0A1U7ETN1_NATPD</name>
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        <submittedName>
            <fullName evidence="1">START domain protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ordered locus">NP_0376A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321917.1">
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETN1"/>
    <dbReference type="STRING" id="348780.NP_0376A"/>
    <dbReference type="EnsemblBacteria" id="CAI48279">
        <property type="protein sequence ID" value="CAI48279"/>
        <property type="gene ID" value="NP_0376A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700834"/>
    <dbReference type="KEGG" id="nph:NP_0376A"/>
    <dbReference type="eggNOG" id="arCOG01927">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1830640_0_0_2"/>
    <dbReference type="OrthoDB" id="25755at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07812">
        <property type="entry name" value="SRPBCC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019587">
        <property type="entry name" value="Polyketide_cyclase/dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10604">
        <property type="entry name" value="Polyketide_cyc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="146" mass="16606" checksum="73C9E905DDFAB137" modified="2017-05-10" version="1">MTVRVERVFDIPVPPEGVWSVIADPKTRAESISVVEEYETTGDRTAVWHVSLPIPRVDRTIRIETEDIERDEPEYVKFTGRSKAMYVQGEHELEPTDEGTRLTNRFVVDGRLPGVETYFKRKLDDELANLEAVVLESVNTEGTDDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETR8</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0474A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETR8"/>
    <dbReference type="EnsemblBacteria" id="CAI48328">
        <property type="protein sequence ID" value="CAI48328"/>
        <property type="gene ID" value="NP_0474A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700869"/>
    <dbReference type="KEGG" id="nph:NP_0474A"/>
    <dbReference type="eggNOG" id="arCOG06432">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098905_0_0_2"/>
    <dbReference type="OrthoDB" id="325775at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="67"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="173" mass="17869" checksum="4645875B7FE01784" modified="2017-05-10" version="1">MALPNAANTSPTTTRRRLLIGTGAAAATATAGCTTVVNWLADMALADVNLFNETDGELAGTITVTGPDGGTLLDESFELLPGDDDDNNGDGDENGGEDDGSNLAAYSDVWTDPGEYEATVTVTDGPLEGSEATETVTIDDTEEEMLAVPLGAEEETGDIVFRVGTDLTDFADE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU09</accession>
    <name>A0A1U7EU09_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABCE1 family ribosome recycling factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">NP_0678A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322066.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU09"/>
    <dbReference type="STRING" id="348780.NP_0678A"/>
    <dbReference type="EnsemblBacteria" id="CAI48430">
        <property type="protein sequence ID" value="CAI48430"/>
        <property type="gene ID" value="NP_0678A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700927"/>
    <dbReference type="KEGG" id="nph:NP_0678A"/>
    <dbReference type="eggNOG" id="arCOG00187">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017344_4_1_2"/>
    <dbReference type="OrthoDB" id="30658at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03236">
        <property type="entry name" value="ABC_RNaseL_inhibitor_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001546">
        <property type="entry name" value="RNase L inhibitor homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013283">
        <property type="entry name" value="RLI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034348">
        <property type="entry name" value="RLI_dom_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007209">
        <property type="entry name" value="RNaseL-inhib-like_metal-bd_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009945">
        <property type="entry name" value="PRK13409.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19248">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04068">
        <property type="entry name" value="Fer4_RLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01868">
        <property type="entry name" value="ABCEFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="55"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="81"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="357"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="579"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="592"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI48430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="612" mass="68227" checksum="8AC62DD76AA30B10" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUA6</accession>
    <name>A0A1U7EUA6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 15">Archaeal Lon protease</fullName>
            <ecNumber evidence="15">3.4.21.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">ATP-dependent protease La homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">lon</name>
        <name evidence="19" type="ordered locus">NP_0910A</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Degrades polypeptides processively.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13 15">Homohexamer. Organized in a ring with a central cavity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Cell membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the peptidase S16 family. Archaeal LonB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="15"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322182.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUA6"/>
    <dbReference type="STRING" id="348780.NP_0910A"/>
    <dbReference type="EnsemblBacteria" id="CAI48546">
        <property type="protein sequence ID" value="CAI48546"/>
        <property type="gene ID" value="NP_0910A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702888"/>
    <dbReference type="KEGG" id="nph:NP_0910A"/>
    <dbReference type="eggNOG" id="arCOG02160">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_392630_0_0_2"/>
    <dbReference type="OrthoDB" id="64652at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004663">
        <property type="entry name" value="Lon_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046843">
        <property type="entry name" value="LonB_AAA-LID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000523">
        <property type="entry name" value="Mg_chelatse_chII-like_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008268">
        <property type="entry name" value="Peptidase_S16_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002078">
        <property type="entry name" value="Sigma_54_int"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00764">
        <property type="entry name" value="lon_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10046">
        <property type="entry name" value="ATP DEPENDENT LON PROTEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20436">
        <property type="entry name" value="LonB_AAA-LID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01078">
        <property type="entry name" value="Mg_chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00158">
        <property type="entry name" value="Sigma54_activat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00830">
        <property type="entry name" value="ENDOLAPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01046">
        <property type="entry name" value="LON_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50045">
        <property type="entry name" value="SIGMA54_INTERACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 15" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 14 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 14 15" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 14 15" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="214"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma-54 factor interaction" evidence="17">
        <location>
            <begin position="278"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="Lon proteolytic" evidence="18">
        <location>
            <begin position="514"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="31"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="67"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="active site" evidence="14">
        <location>
            <position position="599"/>
        </location>
    </feature>
    <feature type="active site" evidence="14">
        <location>
            <position position="642"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00026070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU369001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAI48546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="725" mass="78765" checksum="12C2668F2FFA8A50" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUC3</accession>
    <name>A0A1U7EUC3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Probable methylated-DNA--protein-cysteine methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.63</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ogt</name>
        <name evidence="4" type="ordered locus">NP_0918A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.63" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48550.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322186.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUC3"/>
    <dbReference type="STRING" id="348780.NP_0918A"/>
    <dbReference type="EnsemblBacteria" id="CAI48550">
        <property type="protein sequence ID" value="CAI48550"/>
        <property type="gene ID" value="NP_0918A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703002"/>
    <dbReference type="KEGG" id="nph:NP_0918A"/>
    <dbReference type="eggNOG" id="arCOG02724">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1700230_0_0_2"/>
    <dbReference type="OrthoDB" id="372118at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003908">
        <property type="term" value="F:methylated-DNA-[protein]-cysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014048">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036217">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNAb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01035">
        <property type="entry name" value="DNA_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46767">
        <property type="entry name" value="Methylated DNA-protein cysteine methyltransferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methylated-DNA-[protein]-cysteine S-methyltransferase DNA binding" evidence="3">
        <location>
            <begin position="77"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="128"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48550.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="155" mass="16844" checksum="79452C27769F8B62" modified="2017-05-10" version="1">MDVTPEAGIYARESPYLQRHVQVGVAGGRVLSVSFPETPDADAETDHQLLDRIEVYLDGEADDFADVEVAMTMPTDQRSVLETLRSVPYGETTTVEALTQMTAGLDPEENDDRRLVRTALAENPAPLVVGDHRVSDGPGAAPAAVEQRLRSLEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUH0</accession>
    <name>A0A1U7EUH0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NikR family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">NP_1048A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transcriptional regulatory CopG/NikR family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322250.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUH0"/>
    <dbReference type="EnsemblBacteria" id="CAI48615">
        <property type="protein sequence ID" value="CAI48615"/>
        <property type="gene ID" value="NP_1048A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701038"/>
    <dbReference type="KEGG" id="nph:NP_1048A"/>
    <dbReference type="eggNOG" id="arCOG01008">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113319_2_0_2"/>
    <dbReference type="OrthoDB" id="25654at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22231">
        <property type="entry name" value="RHH_NikR_HicB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1150">
        <property type="entry name" value="ACT-like. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="entry name" value="Met repressor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027271">
        <property type="entry name" value="Acetolactate_synth/TF_NikR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002145">
        <property type="entry name" value="CopG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050192">
        <property type="entry name" value="CopG/NikR_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014864">
        <property type="entry name" value="TF_NikR_Ni-bd_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34719">
        <property type="entry name" value="NICKEL-RESPONSIVE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34719:SF2">
        <property type="entry name" value="NICKEL-RESPONSIVE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08753">
        <property type="entry name" value="NikR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01402">
        <property type="entry name" value="RHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="Ribbon-helix-helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Ribbon-helix-helix protein CopG" evidence="5">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription factor NikR nickel binding C-terminal" evidence="6">
        <location>
            <begin position="55"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="126" mass="14359" checksum="C9598A4C9B7604A0" modified="2017-05-10" version="1">MRTSLAVPEELLSEFDATWQKEGLDSRSRAIREAMAEYVERHEALETADGPVAGVVVFDYEHEEVIENLHGVQHTYQDVIVSTSHAHRGEWCLETLFVDGDAAAIRELVYQLKDFDGVRRVRTMVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUI2</accession>
    <name>A0A1U7EUI2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Sirohydrochlorin cobaltochelatase</fullName>
            <ecNumber evidence="5">4.99.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cbiX1</name>
        <name evidence="5" type="ordered locus">NP_1108A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.99.1.3" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUI2"/>
    <dbReference type="STRING" id="348780.NP_1108A"/>
    <dbReference type="EnsemblBacteria" id="CAI48645">
        <property type="protein sequence ID" value="CAI48645"/>
        <property type="gene ID" value="NP_1108A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_1108A"/>
    <dbReference type="eggNOG" id="arCOG04750">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056929_0_0_2"/>
    <dbReference type="OrthoDB" id="297477at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016852">
        <property type="term" value="F:sirohydrochlorin cobaltochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03414">
        <property type="entry name" value="CbiX_SirB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03416">
        <property type="entry name" value="CbiX_SirB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02980">
        <property type="entry name" value="TRX_Fd_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1400">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002762">
        <property type="entry name" value="CbiX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050963">
        <property type="entry name" value="Sirohydro_Cobaltochel/CbiX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33542">
        <property type="entry name" value="SIROHYDROCHLORIN FERROCHELATASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33542:SF3">
        <property type="entry name" value="SIROHYDROCHLORIN FERROCHELATASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01903">
        <property type="entry name" value="CbiX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53800">
        <property type="entry name" value="Chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="4" id="KW-0170">Cobalt</keyword>
    <keyword evidence="3 5" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI48645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="396" mass="42744" checksum="E2A34391008A81CB" modified="2017-05-10" version="1">MLDAEAVVLAGHGSRREKSNEQVRTLAAGLEGRLGFPVTAGFIELAEPSIPEAIESVAPSVSDVTVIPLSLFAASHVKADVPLVVNEARTEHTDVNVHNGRHLGVHPAIVELLDDRAAAVEAELNVDREDDDVVVVLCARGSSDPDSNGDVHKLARLLYEGRAFADVEAAFIGVTEPLLEPALHTVAKRRPDAVVVLPYMLGDGVLTERIRETTATFDDEYRYAEAACGDPLGTDDRLLTVLADRFEEARTGDVSMSCDTCKYKVELDGFEGEAGGARAMLRAMTHQAAHADRSDIDEEPHVHDAPDKHVTVCTNRTCADAGAATVLERLRQAVRDRGIDARITRSSCLDRCGDGPNVAVYPDNVWYGGVAPEDVDRIASSLERDRIVSELVDQTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUL0</accession>
    <name>A0A1U7EUL0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1150A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322302.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUL0"/>
    <dbReference type="STRING" id="348780.NP_1150A"/>
    <dbReference type="EnsemblBacteria" id="CAI48666">
        <property type="protein sequence ID" value="CAI48666"/>
        <property type="gene ID" value="NP_1150A"/>
    </dbReference>
    <dbReference type="GeneID" id="77383457"/>
    <dbReference type="KEGG" id="nph:NP_1150A"/>
    <dbReference type="eggNOG" id="arCOG07919">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_172992_1_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="41" mass="4687" checksum="83F6735A9C4EE2E6" modified="2017-05-10" version="1">MTETREENGDDVDTSHLDNVEDGCGCTEIWEHMSDERDDAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUQ0</accession>
    <name>A0A1U7EUQ0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Methylmalonyl-CoA epimerase</fullName>
            <ecNumber evidence="4">5.1.99.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">mce</name>
        <name evidence="4" type="synonym">glo2</name>
        <name evidence="4" type="ordered locus">NP_1228A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methylmalonyl-CoA epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.99.1" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322341.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUQ0"/>
    <dbReference type="STRING" id="348780.NP_1228A"/>
    <dbReference type="EnsemblBacteria" id="CAI48705">
        <property type="protein sequence ID" value="CAI48705"/>
        <property type="gene ID" value="NP_1228A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702757"/>
    <dbReference type="KEGG" id="nph:NP_1228A"/>
    <dbReference type="eggNOG" id="arCOG02706">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046006_5_2_2"/>
    <dbReference type="OrthoDB" id="6161at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004493">
        <property type="term" value="F:methylmalonyl-CoA epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046491">
        <property type="term" value="P:L-methylmalonyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07249">
        <property type="entry name" value="MMCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017515">
        <property type="entry name" value="MeMalonyl-CoA_epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051785">
        <property type="entry name" value="MMCE/EMCE_epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03081">
        <property type="entry name" value="metmalonyl_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43048">
        <property type="entry name" value="METHYLMALONYL-COA EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43048:SF3">
        <property type="entry name" value="METHYLMALONYL-COA EPIMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13669">
        <property type="entry name" value="Glyoxalase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="3">
        <location>
            <begin position="2"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="127" mass="13983" checksum="AA7CFC8D33E78600" modified="2017-05-10" version="1">MRFDHAGVATDDAEGLAFLYEDLFGCSIAHEERFGELKVLFLELENGYFELLEPQDDGTIASYLDEHGPGLHHIALETTDINAALETATDLGVECIDEAPRQGAWGHQVAFLHPKSTGGVLIEYVEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUX2</accession>
    <name>A0A1U7EUX2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UspA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_1430A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322441.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUX2"/>
    <dbReference type="STRING" id="348780.NP_1430A"/>
    <dbReference type="EnsemblBacteria" id="CAI48806">
        <property type="protein sequence ID" value="CAI48806"/>
        <property type="gene ID" value="NP_1430A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701148"/>
    <dbReference type="KEGG" id="nph:NP_1430A"/>
    <dbReference type="eggNOG" id="arCOG02053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_11_0_2"/>
    <dbReference type="OrthoDB" id="105697at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF6">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN UP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="156" mass="16556" checksum="0015A523C14472C1" modified="2017-05-10" version="1">MFETVVVATDGSASVSRAVDCTLDLASKFDATVHALYVVDEGHLEELPTDLEGDIRDGLDEQGQQALNDIVAANERREDPVELETAVRVGHPEKQIVGYVRDVDADGIAMGTRGRSGEHSFLLGSVAERVVRTCPVPVLTVRQLEGAADTAEAAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUX7</accession>
    <name>A0A1U7EUX7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DUF83 domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1424A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322438.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUX7"/>
    <dbReference type="STRING" id="348780.NP_1424A"/>
    <dbReference type="EnsemblBacteria" id="CAI48803">
        <property type="protein sequence ID" value="CAI48803"/>
        <property type="gene ID" value="NP_1424A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701146"/>
    <dbReference type="KEGG" id="nph:NP_1424A"/>
    <dbReference type="eggNOG" id="arCOG00800">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088481_0_0_2"/>
    <dbReference type="OrthoDB" id="26676at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="219" mass="24707" checksum="20BDB25CE087FDE6" modified="2017-05-10" version="1">MEAFTDLATAAFCPRKLYYRRKHDDATPPDDVAATRNLAFRYSSLLNAESSLDAEPVVPEPSAYREALRRQRQRLDRWPDLVDPPSTRVFLSGKDAHGIAHKVLESPPAPAIVSTGKPPENGVWKPHSVRATAAAKALSWERQQPVETAYVEYPAFGDIRKVRMTTRRKATYRKALNTAESIDGPPPRLKNDNRCQTCEYADECGTRTRSLRSRLSRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV03</accession>
    <name>A0A1U7EV03_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ferritin domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1498A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48840.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939805.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV03"/>
    <dbReference type="STRING" id="348780.NP_1498A"/>
    <dbReference type="EnsemblBacteria" id="CAI48840">
        <property type="protein sequence ID" value="CAI48840"/>
        <property type="gene ID" value="NP_1498A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701162"/>
    <dbReference type="KEGG" id="nph:NP_1498A"/>
    <dbReference type="eggNOG" id="arCOG06430">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1485876_0_0_2"/>
    <dbReference type="OrthoDB" id="304955at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48840.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="161" mass="18481" checksum="E958734F4D05151D" modified="2017-05-10" version="1">MSLTYPVATDHQLARLLQIGMVLEEVVEARAHKHRETLDAEELDPETADLLDEAAEESETHRKRLESLVDELDAEPVAYDEIETLVAARYESDTDFDGVLYDQLCNEETAYKFYDDLITAIEGSDVEFSIDRERLLSVLREIRADEKEGAEQVTELMEERE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV18</accession>
    <name>A0A1U7EV18_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA double-strand break repair Rad50 ATPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 14" type="primary">rad50</name>
        <name evidence="14" type="ordered locus">NP_1516A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Part of the Rad50/Mre11 complex, which is involved in the early steps of DNA double-strand break (DSB) repair. Rad50 controls the balance between DNA end bridging and DNA resection via ATP-dependent structural rearrangements of the Rad50/Mre11 complex.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10">Binds 1 zinc ion per homodimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer. Forms a heterotetramer composed of two Mre11 subunits and two Rad50 subunits.</text>
    </comment>
    <comment type="domain">
        <text evidence="10">The two conserved Cys that bind zinc constitute the zinc-hook, which separates the large intramolecular coiled coil regions. The 2 Cys residues coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another Rad50 molecule, thereby forming a V-shaped homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the SMC family. RAD50 subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the Sph1/Sph2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322483.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV18"/>
    <dbReference type="STRING" id="348780.NP_1516A"/>
    <dbReference type="EnsemblBacteria" id="CAI48849">
        <property type="protein sequence ID" value="CAI48849"/>
        <property type="gene ID" value="NP_1516A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703094"/>
    <dbReference type="KEGG" id="nph:NP_1516A"/>
    <dbReference type="eggNOG" id="arCOG00368">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004785_0_1_2"/>
    <dbReference type="OrthoDB" id="25344at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
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    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00449">
        <property type="entry name" value="RAD50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053480">
        <property type="entry name" value="DSB_repair_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="Rad50/SbcC_AAA"/>
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    <dbReference type="InterPro" id="IPR022982">
        <property type="entry name" value="Rad50_ATPase_archaeal"/>
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    <dbReference type="InterPro" id="IPR013134">
        <property type="entry name" value="Zn_hook_RAD50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002572">
        <property type="entry name" value="PRK02224.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041035">
        <property type="entry name" value="Rad50_Halo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32114">
        <property type="entry name" value="ABC TRANSPORTER ABCH.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32114:SF2">
        <property type="entry name" value="ABC TRANSPORTER ABCH.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75712">
        <property type="entry name" value="Rad50 coiled-coil Zn hook"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51131">
        <property type="entry name" value="ZN_HOOK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zinc-hook" evidence="13">
        <location>
            <begin position="417"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="336"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="514"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="351"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="373"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="400"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="413"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="525"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="32"/>
            <end position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 11">
        <location>
            <position position="464"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 11">
        <location>
            <position position="467"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAI48849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="897" mass="101736" checksum="9957AB76C2CC76CF" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV86</accession>
    <name>A0A1U7EV86_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Pyridoxal phosphate-dependent aminotransferase</fullName>
            <ecNumber evidence="8">2.6.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">aspC2</name>
        <name evidence="8" type="ordered locus">NP_1666A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
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    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV86"/>
    <dbReference type="STRING" id="348780.NP_1666A"/>
    <dbReference type="EnsemblBacteria" id="CAI48924">
        <property type="protein sequence ID" value="CAI48924"/>
        <property type="gene ID" value="NP_1666A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702534"/>
    <dbReference type="KEGG" id="nph:NP_1666A"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="372018at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050596">
        <property type="entry name" value="AspAT/PAT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383:SF3">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="7">
        <location>
            <begin position="26"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI48924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="373" mass="39777" checksum="BA591C8235DE990F" modified="2017-05-10" version="1">MTDFSDRVERVSISGIREVFEAAGDDAINLGLGQPDFPTPDHVREAAIEAIEAGDVDAYTSNKGTRELREAIAAKYDRDNDFAVDPENIVATSGGSEALHLAIEAHVDAGEEVIFPDPGFVSYDALTHIAGGEPNPVSLRDDLTLSPADVEAAITDDTAMFVVNSPANPTGAVQSEADMREFARIAADHDVVCLSDEVYEPFVFDGVHRSPAAFDETGNVVVVNAASKAYSMTGWRLGWVTGTSERIDRMLRVHQYAQACASAPAQYAAEAALSGPQEPVEEMRSAFETRRDVLVDGLEDMGLSVPTPKGAFYAMPEVPDGWTDEVIDRGVVVVPGEAFGDAGAGHARISYAVDVETLKDALEVMGEATAALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVG4</accession>
    <name>A0A1U7EVG4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Probable secreted glycoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_1800A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVG4"/>
    <dbReference type="STRING" id="348780.NP_1800A"/>
    <dbReference type="EnsemblBacteria" id="CAI48991">
        <property type="protein sequence ID" value="CAI48991"/>
        <property type="gene ID" value="NP_1800A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_1800A"/>
    <dbReference type="eggNOG" id="arCOG02087">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG03256">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_266656_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.680">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1120">
        <property type="entry name" value="Carboxypeptidase-like, regulatory domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013784">
        <property type="entry name" value="Carb-bd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008969">
        <property type="entry name" value="CarboxyPept-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008965">
        <property type="entry name" value="CBM2/CBM3_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13620">
        <property type="entry name" value="CarboxypepD_reg"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49384">
        <property type="entry name" value="Carbohydrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49464">
        <property type="entry name" value="Carboxypeptidase regulatory domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49452">
        <property type="entry name" value="Starch-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1208"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="642"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1181"/>
            <end position="1201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="646"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="663"/>
            <end position="672"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="1241" mass="129272" checksum="E73D2CA9E3F1C11C" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVI6</accession>
    <name>A0A1U7EVI6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">AI-2E family transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">NP_1894A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322670.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVI6"/>
    <dbReference type="STRING" id="348780.NP_1894A"/>
    <dbReference type="EnsemblBacteria" id="CAI49038">
        <property type="protein sequence ID" value="CAI49038"/>
        <property type="gene ID" value="NP_1894A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701295"/>
    <dbReference type="KEGG" id="nph:NP_1894A"/>
    <dbReference type="eggNOG" id="arCOG02642">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041771_2_0_2"/>
    <dbReference type="OrthoDB" id="137390at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002549">
        <property type="entry name" value="AI-2E-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 245"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01594">
        <property type="entry name" value="AI-2E_transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="62"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="201"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="232"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="300"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="344"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="379"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="403"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="414" mass="43021" checksum="D7D52B34C6A20957" modified="2017-05-10" version="1">MDDGDDLSEWFSDRLTLTVVTAVSVVLGLLVVIPYLEYVLLGAVLAYVLLPVQRRLEAHVGRLVAAVSISVATVLAFLLPLLTVLAIALREAVAVATALQRGVVDVPGIELWVEETTGFEVDLVNLYSTYEGQIASAIQSIVTRAMRVVGGIPSLLIGLTVTVFVLFALLRDRGKLLAWLYRVLPVDDGVQQELFRELDRLMWASVVGNVAVAAIQAVLLGVALVLLDVPVVVFLTVTTFVLSLLPLVGAFGVWVPVSVYLAVADRPTAAALLVVYGSLVSLSDNYFRPALIGRSGALNPAIIVVGIFGGIVAFGAVGLFIGPVVLGGAKVTLDVFARERAAATGRTAPEWDGDDAVAGSPTDGDAANSGNDGEQGEGAADDADAGGEGDADGEAAASTATDAGDEADDNDDRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVP5</accession>
    <name>A0A1U7EVP5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7552 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2076A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322757.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVP5"/>
    <dbReference type="STRING" id="348780.NP_2076A"/>
    <dbReference type="EnsemblBacteria" id="CAI49129">
        <property type="protein sequence ID" value="CAI49129"/>
        <property type="gene ID" value="NP_2076A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701344"/>
    <dbReference type="KEGG" id="nph:NP_2076A"/>
    <dbReference type="eggNOG" id="arCOG06244">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2299419_0_0_2"/>
    <dbReference type="OrthoDB" id="342580at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055974">
        <property type="entry name" value="DUF7552"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24422">
        <property type="entry name" value="DUF7552"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7552" evidence="2">
        <location>
            <begin position="6"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="100" mass="11404" checksum="ADD17FC433288F23" modified="2017-05-10" version="1">MNERMLRALRRHIESLADPDGTYVVCSRTGERPVPADGLRFRDCLTAREAARLAAQYRTRLRRYDRRLPRYDLTVRQSPGDSPLATTDSDADRPAVTTKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVP6</accession>
    <name>A0A1U7EVP6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">rRNA/tRNA 2'-O-methyltransferase complex protein Nop5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">nop5</name>
        <name evidence="2" type="ordered locus">NP_2064A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322752.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVP6"/>
    <dbReference type="STRING" id="348780.NP_2064A"/>
    <dbReference type="EnsemblBacteria" id="CAI49123">
        <property type="protein sequence ID" value="CAI49123"/>
        <property type="gene ID" value="NP_2064A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702981"/>
    <dbReference type="KEGG" id="nph:NP_2064A"/>
    <dbReference type="eggNOG" id="arCOG01923">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015495_1_1_2"/>
    <dbReference type="OrthoDB" id="11877at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.90">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045056">
        <property type="entry name" value="Nop56/Nop58"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042239">
        <property type="entry name" value="Nop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002687">
        <property type="entry name" value="Nop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036070">
        <property type="entry name" value="Nop_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894">
        <property type="entry name" value="NUCLEOLAR PROTEIN 5 NUCLEOLAR PROTEIN NOP5 NOP58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01798">
        <property type="entry name" value="Nop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89124">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51358">
        <property type="entry name" value="NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nop" evidence="1">
        <location>
            <begin position="162"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="280" mass="29910" checksum="8D5CFBA75492831E" modified="2017-05-10" version="1">MTDERAVEDGWFADVPPGDVEAAAAAIRDGSAATVGEWPSLAVESGVAADEDDYYEQLHEAAVAATRAAVHEAETADDQQLIHAVRAMDDTERVANELAERVTEWAGDRYDADGTGVEYAASLADADDLDDPQLRSLAERIRDLAAEADDLRSHVERVTPEIAPNLARLAGPVLAARLIALAGGLKQLARKPAGTVQVLGAEEALFAHLRGRAPSPKHGVIYTHEAIQGTAPAHRGSAARALAGKLTIAARIDHYSGERNPELEADLRDRIERIQARDAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVQ2</accession>
    <name>A0A1U7EVQ2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Flavin-dependent pyridine nucleotide oxidoreductase (Homolog to coenzyme A disulfide reductase)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">NP_2052A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-III pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322746.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVQ2"/>
    <dbReference type="STRING" id="348780.NP_2052A"/>
    <dbReference type="EnsemblBacteria" id="CAI49117">
        <property type="protein sequence ID" value="CAI49117"/>
        <property type="gene ID" value="NP_2052A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701336"/>
    <dbReference type="KEGG" id="nph:NP_2052A"/>
    <dbReference type="eggNOG" id="arCOG01069">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003291_1_3_2"/>
    <dbReference type="OrthoDB" id="27922at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050260">
        <property type="entry name" value="FAD-bd_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43429:SF1">
        <property type="entry name" value="NAD(P)H SULFUR OXIDOREDUCTASE (COA-DEPENDENT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43429">
        <property type="entry name" value="PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="8">
        <location>
            <begin position="4"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="7">
        <location>
            <begin position="348"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI49117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="469" mass="48752" checksum="EBD725A5D921F266" modified="2017-05-10" version="1">MSTFVVIGGDAAGMSAASKAKRDTPDLDVVVFEKGAWVSYGACGLPYYIKGEIQTLEDLVSVTPEQFREERDIDLRTGHEVVDIDPDDRTVTARGDGEELVQPYDSLLIATGAAAVVPDIEGVDREGAYTLGSMTDGKELREFVARAREEGPAEQPDSGPACQQLENCNGPVGVVGGGYIGIEMAEALAANDFEVHLFQRSDRVLKAFAPETSAAVADHLESQSVSLYLGSEVQALDGDDGVEAIVTGDGERVPVEMALLGAGVRPRTDLAAAAGIELGPTGAIATDAYRETNIDDVYAAGDCAEAEHVVTGEPTYVPLALTANRHGRAIGQTVAGTPTKGGGVAGTAAVKAFEMEAARTGLSHDDAAAAGFEPVTETIEAKSRAGYYPGGGTVTVTLTADSETGRLLGGTLHSEYGEGAVHRSHGLVGAVTEGVTLSELSNYDFAYAPPFNTTWDPILTAAKVLDGTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVR0</accession>
    <name>A0A1U7EVR0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flagellin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">flg3</name>
        <name evidence="7" type="ordered locus">NP_2090A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Flagellin is the subunit protein which polymerizes to form the filaments of archaeal flagella.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Archaeal flagellum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the archaeal flagellin family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322764.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVR0"/>
    <dbReference type="STRING" id="348780.NP_2090A"/>
    <dbReference type="EnsemblBacteria" id="CAI49136">
        <property type="protein sequence ID" value="CAI49136"/>
        <property type="gene ID" value="NP_2090A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702720"/>
    <dbReference type="KEGG" id="nph:NP_2090A"/>
    <dbReference type="eggNOG" id="arCOG01829">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051124_1_0_2"/>
    <dbReference type="OrthoDB" id="102632at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097589">
        <property type="term" value="C:archaeal-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097588">
        <property type="term" value="P:archaeal or bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013373">
        <property type="entry name" value="Flagellin/pilin_N_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002774">
        <property type="entry name" value="Flagellin_arc-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02537">
        <property type="entry name" value="arch_flag_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35903">
        <property type="entry name" value="FLAGELLIN B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35903:SF1">
        <property type="entry name" value="FLAGELLIN B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01917">
        <property type="entry name" value="Flagellin_arch-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0974">Archaeal flagellum</keyword>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="7" id="KW-0282">Flagellum</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="178"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="210" mass="22130" checksum="02A2DA6215DAD2CF" modified="2017-05-10" version="1">MFEQFTESEERGQVGIGTLIVFIALVLVAAIAAGVLINTAGFLQTQAQATGEESTEQVSTNLVYLSTTGTVSNSEDGINQFETTVQLGPGSSAIDLNDTTISVFTDDGESEEFDSLAEDGIELEDRSDDEAVLSTASSDDDATATIVFDLTDGDGIDPEDGQFDEGTTVDIVITTAEGTQAENSFIIEDPLDPQLDGNTDPDQADEIRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVT1</accession>
    <name>A0A1U7EVT1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2136A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322787.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVT1"/>
    <dbReference type="STRING" id="348780.NP_2136A"/>
    <dbReference type="EnsemblBacteria" id="CAI49159">
        <property type="protein sequence ID" value="CAI49159"/>
        <property type="gene ID" value="NP_2136A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701361"/>
    <dbReference type="KEGG" id="nph:NP_2136A"/>
    <dbReference type="eggNOG" id="arCOG04581">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143266_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="152" mass="15987" checksum="3B4D049670C15114" modified="2017-05-10" version="1">MVVDTVMYTLHTAFGALWAGSVLFVVLAVLPLAMNAKIDPGAFASVVSKLQWITRISAVVLLATGGHLAGTRYTAERLTGTTPGYVVLAMLALWLLLAAVVEIGSARANRGLAEDKIREPARNARPFYLLGAVFAVGLLVTAGLLGEPSLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVU3</accession>
    <name>A0A1U7EVU3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fla cluster protein FlaH</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">flaH</name>
        <name evidence="4" type="ordered locus">NP_2156A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322797.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVU3"/>
    <dbReference type="STRING" id="348780.NP_2156A"/>
    <dbReference type="EnsemblBacteria" id="CAI49169">
        <property type="protein sequence ID" value="CAI49169"/>
        <property type="gene ID" value="NP_2156A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702714"/>
    <dbReference type="KEGG" id="nph:NP_2156A"/>
    <dbReference type="eggNOG" id="arCOG04148">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094838_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014774">
        <property type="entry name" value="KaiC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637:SF3">
        <property type="entry name" value="FLAGELLA-RELATED PROTEIN H-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637">
        <property type="entry name" value="UPF0273 PROTEIN TM_0370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06745">
        <property type="entry name" value="ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KaiC-like" evidence="3">
        <location>
            <begin position="19"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="253" mass="27672" checksum="145362F5F83666E0" modified="2017-05-10" version="1">MQKTRNLHSLGLDDHDRLNKELGGGIPKGSIVLAEGDYGAGKSALSQRMAYGFCEEGTTVTFLSTELTVSGFLDQMNSLSYDVVDHVLDERLLFLHADIDTGGVLSGGSDDEESRMDLLTRLMEAETMWTADVIIIDTFDAVLRNDPTFEALIRQNEERQAALEIISFFRDMISEGKVIVLTVDPSTVDGDAIGPFRAIADVFLELEMIEVGNDVRRQISVKRFAGMGEQVGDTIGFSVRSGTGIVIESRSIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVU7</accession>
    <name>A0A1U7EVU7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Triosephosphate isomerase</fullName>
            <shortName evidence="5">TIM</shortName>
            <shortName evidence="5">TPI</shortName>
            <ecNumber evidence="5">5.3.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Triose-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">tpiA1</name>
        <name evidence="5" type="synonym">tpiA</name>
        <name evidence="6" type="ordered locus">NP_2182A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:18585"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="5.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the triosephosphate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.1" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322810.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVU7"/>
    <dbReference type="STRING" id="348780.NP_2182A"/>
    <dbReference type="EnsemblBacteria" id="CAI49182">
        <property type="protein sequence ID" value="CAI49182"/>
        <property type="gene ID" value="NP_2182A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703357"/>
    <dbReference type="KEGG" id="nph:NP_2182A"/>
    <dbReference type="eggNOG" id="arCOG01087">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104921_0_0_2"/>
    <dbReference type="OrthoDB" id="9465at2157"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00189"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004807">
        <property type="term" value="F:triose-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00147_A">
        <property type="entry name" value="TIM_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035990">
        <property type="entry name" value="TIM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000652">
        <property type="entry name" value="Triosephosphate_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022891">
        <property type="entry name" value="Triosephosphate_isomerase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020861">
        <property type="entry name" value="Triosephosphate_isomerase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003302">
        <property type="entry name" value="PRK04302.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00419">
        <property type="entry name" value="tim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00121">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51351">
        <property type="entry name" value="Triosephosphate isomerase (TIM)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00171">
        <property type="entry name" value="TIM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51440">
        <property type="entry name" value="TIM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 5" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="3 5" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="4 5 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Electrophile" evidence="5">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="6"/>
            <end position="8"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="193"/>
            <end position="194"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI49182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="215" mass="21468" checksum="5B8FFE64B926C1E2" modified="2017-05-10" version="1">MFVVVNLKAYPCDPVAVAEAAADVADETGVRIAVAPQAADISRVAETGVETWAQHVDAVDHGSHTGSTLAEAAAEAGAEGTLLNHSEKRLRLADIDGGLRAAERVGLESIVCANNPRQIAAAAALEPDAVAVEPPALIGTGTPVSQADPEVVTDSVEAADAVGDVDVLCGAGISTGDDLEAAAELGAEGVLLASGVAKADAPKAALRDLVEPIAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVX1</accession>
    <name>A0A1U7EVX1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7123 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2266A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322851.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVX1"/>
    <dbReference type="STRING" id="348780.NP_2266A"/>
    <dbReference type="EnsemblBacteria" id="CAI49224">
        <property type="protein sequence ID" value="CAI49224"/>
        <property type="gene ID" value="NP_2266A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701396"/>
    <dbReference type="KEGG" id="nph:NP_2266A"/>
    <dbReference type="eggNOG" id="arCOG02866">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_184952_0_0_2"/>
    <dbReference type="OrthoDB" id="380315at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055547">
        <property type="entry name" value="DUF7123"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23438">
        <property type="entry name" value="DUF7123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7123" evidence="2">
        <location>
            <begin position="20"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="94" mass="10568" checksum="38A24B8124DBF888" modified="2017-05-10" version="1">MNTHAKSPSTTTPNRPSESPYRSHAAKRLAQYLRTELERADEALYVTGDRIADDVGLPPLEVERLLRQLSDRVPGLSIRTDPSGSRTVWRVSRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW87</accession>
    <name>A0A1U7EW87_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ubiquitin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2498A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322966.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW87"/>
    <dbReference type="STRING" id="348780.NP_2498A"/>
    <dbReference type="EnsemblBacteria" id="CAI49340">
        <property type="protein sequence ID" value="CAI49340"/>
        <property type="gene ID" value="NP_2498A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701466"/>
    <dbReference type="KEGG" id="nph:NP_2498A"/>
    <dbReference type="eggNOG" id="arCOG06308">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_160444_0_0_2"/>
    <dbReference type="OrthoDB" id="263424at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.80">
        <property type="entry name" value="Molybdopterin cofactor biosynthesis MoaD-related, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015272">
        <property type="entry name" value="MoadD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036473">
        <property type="entry name" value="Mopterin_CF_MoaD-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09189">
        <property type="entry name" value="MoaD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Molybdopterin cofactor biosynthesis MoaD-related C-terminal" evidence="1">
        <location>
            <begin position="3"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="84" mass="9192" checksum="9EDF21B98F76B2F7" modified="2017-05-10" version="1">MEETRTFRGISKRLAISYLEGLGGEHNGESRVEGDGWAATLDARTVSPAGSIRLSEVEIRFEGDRDELDPVIEAFERKAVRAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW90</accession>
    <name>A0A1U7EW90_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2504A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW90"/>
    <dbReference type="STRING" id="348780.NP_2504A"/>
    <dbReference type="EnsemblBacteria" id="CAI49343">
        <property type="protein sequence ID" value="CAI49343"/>
        <property type="gene ID" value="NP_2504A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_2504A"/>
    <dbReference type="HOGENOM" id="CLU_2243938_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="104" mass="10300" checksum="99C341523444F716" modified="2017-05-10" version="1">MRIVPSALGAVKLLIALLFVLIGTVSLAFAGGVVGTGIGPVDDAGDAVVAAAGSAGEAVDEALSAPPAADIEPAVADEVRETRAEHGQSDIRSSDLLQTAAQEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWD9</accession>
    <name>A0A1U7EWD9_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF8009 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2646A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323039.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWD9"/>
    <dbReference type="STRING" id="348780.NP_2646A"/>
    <dbReference type="EnsemblBacteria" id="CAI49414">
        <property type="protein sequence ID" value="CAI49414"/>
        <property type="gene ID" value="NP_2646A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701510"/>
    <dbReference type="KEGG" id="nph:NP_2646A"/>
    <dbReference type="eggNOG" id="arCOG04661">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120319_0_0_2"/>
    <dbReference type="OrthoDB" id="199191at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058322">
        <property type="entry name" value="DUF8009"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26033">
        <property type="entry name" value="DUF8009"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8009" evidence="2">
        <location>
            <begin position="3"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="141" mass="15763" checksum="0D3FE3FFCAC18555" modified="2017-05-10" version="1">MSSTDPTAIRVLAVTAEDLVAAVEANERRDADAVLRVTPPFSGRMRARLHLDGAEHGYESTQPLHIPPERFVSEVPPAPTPDETADTLRNDPDETYTQERHRERHEAVLSAWRQQLKEATLDAAHIETPSGEHEVRVTWLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWF0</accession>
    <name>A0A1U7EWF0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2640A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323036.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWF0"/>
    <dbReference type="STRING" id="348780.NP_2640A"/>
    <dbReference type="EnsemblBacteria" id="CAI49411">
        <property type="protein sequence ID" value="CAI49411"/>
        <property type="gene ID" value="NP_2640A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701508"/>
    <dbReference type="KEGG" id="nph:NP_2640A"/>
    <dbReference type="HOGENOM" id="CLU_2712947_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="72" mass="7416" checksum="DAC66659EB218A41" modified="2017-05-10" version="1">MPPDESGGATVRRYTTRALQLLGVGIAVAVPLLLVVAAVAAEEPGLGLLGAFLLAAAVVLYFSSRPRRPSVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWH2</accession>
    <name>A0A1U7EWH2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Actin-like ATPase involved in cell morphogenesis</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2684A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49433.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323058.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWH2"/>
    <dbReference type="STRING" id="348780.NP_2684A"/>
    <dbReference type="EnsemblBacteria" id="CAI49433">
        <property type="protein sequence ID" value="CAI49433"/>
        <property type="gene ID" value="NP_2684A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701524"/>
    <dbReference type="KEGG" id="nph:NP_2684A"/>
    <dbReference type="eggNOG" id="arCOG04656">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_757812_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057331">
        <property type="entry name" value="Salactin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25229">
        <property type="entry name" value="Salactin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49433.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="433" mass="46985" checksum="2DB713A454EAB1BA" modified="2017-05-10" version="1">MTPNEEPAGRGLSTDDTAEGERTRDGHETATGEQPQDHYGTTDGNVTSGRDRAERGRTRTADEVTEPAGRTSGEAQRRDDVGTAKAEEAADESVVPIGVKLGSTRTVITYPSQSGDGMEVVQSLTCLATYEDLLTGREQILYGEEAAREYPDEVEFMLRSGLPESDDSVESAATFFSELLAANDVPENSVVVYAIPTIDNEAGLSNLEAVVEKSPIGEALVRSYPESLCGALPAFEERLAAINDIFITINMGSTNLEACAYRRGEQLVPLSTGATTGNEADRRIANYVEEETQGRVNIDRTTAREYKEKHADFDSFEPFTDVIQQPGGGTHEFTIEESVMKACDEYLDEVVDEVANGFLPELANNYMKVYQLALDKPLVLTGGMACIPGVVDEFERRLSDELQRDVTAVSADRPDLAAAQGAYEIADHLAQSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWN3</accession>
    <name>A0A1U7EWN3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">CAP domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_2820A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWN3"/>
    <dbReference type="STRING" id="348780.NP_2820A"/>
    <dbReference type="EnsemblBacteria" id="CAI49501">
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        <location>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI49501.1"/>
        </source>
    </evidence>
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        <source>
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    <sequence length="206" mass="22915" checksum="51FD6EEDE7E32B15" modified="2017-05-10" version="1">MNAKTILNIISAVILLFVVLPVVIVGAFELCTTVGNDNNSIDKEEPSNGETKLSGVPNPSSSNHSSIDGERLEDEIHHKINDKRESLDRQRLEKSERVRRIARGHSKSMATGDFYAHVDTDGNDQTDRHEMYDGCTLVGENIIIGHKQDRGVNEIAESLVERWVDSEGHYENLKSQGHNVSGVGVYVTEDGTIYATQNFCREHPDT</sequence>
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        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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                <person name="Pfeiffer F."/>
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    </dbReference>
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        <property type="entry name" value="DUF7976"/>
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        <property type="entry name" value="DUF7976"/>
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        <source>
            <dbReference type="EMBL" id="CAI49504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="76" mass="8713" checksum="5B815D1EF6A7EB46" modified="2017-05-10" version="1">MDDQTHADNERVAMLRAVAEDVRDDSSESEQLAALLYRVSDLYDPKEETTPEDIYRNMRTILRVSEQGGLPERGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWP5</accession>
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        <name evidence="3" type="primary">abc23p1</name>
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        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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                <person name="Rodewald K."/>
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            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
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        <property type="component" value="Chromosome"/>
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        <location>
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        </location>
    </feature>
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        <location>
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        </location>
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    </feature>
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        </location>
    </feature>
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        <location>
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            <end position="512"/>
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    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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            <end position="592"/>
        </location>
    </feature>
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        <location>
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            <end position="626"/>
        </location>
    </feature>
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            <begin position="174"/>
            <end position="222"/>
        </location>
    </feature>
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        <location>
            <begin position="185"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="627" mass="65186" checksum="D87AFB6834991FBF" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWR1</accession>
    <name>A0A1U7EWR1_NATPD</name>
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        <submittedName>
            <fullName evidence="8">Transducer protein Htr28</fullName>
        </submittedName>
    </protein>
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        <name evidence="8" type="primary">htr28</name>
        <name evidence="8" type="ordered locus">NP_2892A</name>
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    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
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    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
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        <property type="protein sequence ID" value="CAI49537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="348780.NP_2892A"/>
    <dbReference type="EnsemblBacteria" id="CAI49537">
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        <property type="gene ID" value="NP_2892A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702844"/>
    <dbReference type="KEGG" id="nph:NP_2892A"/>
    <dbReference type="eggNOG" id="arCOG02318">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="116658at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Methyl-accepting chemotaxis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
    </dbReference>
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        <property type="entry name" value="HAMP_dom"/>
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    </dbReference>
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        <property type="entry name" value="PAS"/>
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        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="PAS_4"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR32089:SF112">
        <property type="entry name" value="LYSOZYME-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32089">
        <property type="entry name" value="METHYL-ACCEPTING CHEMOTAXIS PROTEIN MCPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08448">
        <property type="entry name" value="PAS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00260">
        <property type="entry name" value="CHEMTRNSDUCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58104">
        <property type="entry name" value="Methyl-accepting chemotaxis protein (MCP) signaling domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="PAS" evidence="6">
        <location>
            <begin position="66"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="7">
        <location>
            <begin position="202"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="5">
        <location>
            <begin position="265"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="13"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="504"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="36"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="546" mass="59193" checksum="8F03075D6A089473" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWV0</accession>
    <name>A0A1U7EWV0_NATPD</name>
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        <submittedName>
            <fullName evidence="6">Ba3-type terminal oxidase subunit II</fullName>
            <ecNumber evidence="6">1.9.3.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">cbaB</name>
        <name evidence="6" type="synonym">coxB2</name>
        <name evidence="6" type="ordered locus">NP_2964A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell envelope</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.9.3.-" evidence="6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWV0"/>
    <dbReference type="STRING" id="348780.NP_2964A"/>
    <dbReference type="EnsemblBacteria" id="CAI49573">
        <property type="protein sequence ID" value="CAI49573"/>
        <property type="gene ID" value="NP_2964A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_2964A"/>
    <dbReference type="eggNOG" id="arCOG01236">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120355_0_0_2"/>
    <dbReference type="OrthoDB" id="27522at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13913">
        <property type="entry name" value="ba3_CcO_II_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034214">
        <property type="entry name" value="Ba3_CcO_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002429">
        <property type="entry name" value="CcO_II-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001505">
        <property type="entry name" value="Copper_CuA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051403">
        <property type="entry name" value="NosZ/Cyto_c_oxidase_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42838">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42838:SF2">
        <property type="entry name" value="NITROUS-OXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00116">
        <property type="entry name" value="COX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00078">
        <property type="entry name" value="COX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50857">
        <property type="entry name" value="COX2_CUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0186">Copper</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="9"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome oxidase subunit II copper A binding" evidence="5">
        <location>
            <begin position="65"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI49573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="170" mass="18677" checksum="62D5CDA86DE465FC" modified="2017-05-10" version="1">MNIHTYEKFWLAAAMVLIVGFIATITYGSVGIGIAMVDDEQDTVNPDNLDEHPGFADPGVEQVGENEYEVHIIAVQYAYFPGDFEVPADSEVTFHVTSEDVIHSFSVVGTNANTMVIPGEVASLTVEMPDVDEPTEYGILCNEYCGPGHHDMEALMTVVPEDEFELEDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWX2</accession>
    <name>A0A1U7EWX2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Agmatinase</fullName>
            <ecNumber evidence="6">3.5.3.11</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">speB1</name>
        <name evidence="6" type="ordered locus">NP_3022A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="4">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the arginase family. Agmatinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.3.11" evidence="6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323225.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWX2"/>
    <dbReference type="STRING" id="348780.NP_3022A"/>
    <dbReference type="EnsemblBacteria" id="CAI49602">
        <property type="protein sequence ID" value="CAI49602"/>
        <property type="gene ID" value="NP_3022A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703212"/>
    <dbReference type="KEGG" id="nph:NP_3022A"/>
    <dbReference type="eggNOG" id="arCOG01700">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039478_0_2_2"/>
    <dbReference type="OrthoDB" id="7186at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008783">
        <property type="term" value="F:agmatinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033389">
        <property type="term" value="P:putrescine biosynthetic process from arginine, via agmatine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09990">
        <property type="entry name" value="Agmatinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.10">
        <property type="entry name" value="Ureohydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005925">
        <property type="entry name" value="Agmatinase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020855">
        <property type="entry name" value="Ureohydrolase_Mn_BS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01230">
        <property type="entry name" value="agmatinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358">
        <property type="entry name" value="ARGINASE/AGMATINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358:SF26">
        <property type="entry name" value="GUANIDINO ACID HYDROLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036979">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00116">
        <property type="entry name" value="ARGINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01053">
        <property type="entry name" value="ARGINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR036979-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI49602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="341" mass="35548" checksum="71CF601B6070C518" modified="2017-05-10" version="1">MTQPGDSRAAASRAEHVESGVELAYAGFDTFLKSGPTAVEAAGGADAAVLGVPYDGAVSNRPGARFGPRAVRRASGWLAYLSGYKGGLTNVRTEQQVSFSALDIVDCGDVPVFPMDRERTADSIAAHVATVTARGTMPVVIGGDHYCTYPAFQGFAAASDHSRIGLVQIDAHSDTVDGSAAFGEHFHGSTTHHIAQTAYTDFDHIAQVGLRGYESPDFFEFADENGLSVYTMRDVERRGVRPVIEDAVTAAAADTDAVYVTFDIDAVDPAAAPGTGTPEPGGLTASQALAVMDVLGAHDAVGAADLMEVAPTHDPTGNTARLAAYLLVTLLERQFAEADGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWX3</accession>
    <name>A0A1U7EWX3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">IS1341-type transposase ISNph8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_3036A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323232.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWX3"/>
    <dbReference type="SMR" id="A0A1U7EWX3"/>
    <dbReference type="STRING" id="348780.NP_3036A"/>
    <dbReference type="EnsemblBacteria" id="CAI49609">
        <property type="protein sequence ID" value="CAI49609"/>
        <property type="gene ID" value="NP_3036A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701636"/>
    <dbReference type="KEGG" id="nph:NP_3036A"/>
    <dbReference type="eggNOG" id="arCOG00681">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050175_0_0_2"/>
    <dbReference type="OrthoDB" id="168528at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas12f1-like TNB" evidence="3">
        <location>
            <begin position="301"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="402"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="402"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="423" mass="48913" checksum="F55B0B64FEE8C2D8" modified="2017-05-10" version="1">MQKSLTKTLVFQLDPDESGQQLLDDAFLEARRVYNETIRRTKNGDDWDEIRQDLESDADLVNNTAQLVVQKSLEAMENYYEYDDYNQPSHTKDGAYPLRSNYEEGYNLFLEDDSIRYRISAKPYNPVKGTLQGSLDVLKQLRTAIESETWRVGTAEAMKRNGNYELHINITHTEAEVRDKQDSRTVVGVDINEDCVALASLHEDDIVNSVVIDYPEIKEERHRYFTMRKRMQNSGKSSFDRAFEQQEERFVHDQLHKVSRQVVEWVPRFENPVIVFEDLKEMRDSIDYGTRMNRRLHSLPFRKLREFITYKAAFQGIPSEEINPEYTSQACSLTECEHTTRANRNKKRFKCVECGRQDHADRNAAINIAKKGLEKLNRNVPALNSLPQVRKVRRQASGCVNQPTVTHDTATGHQADGVVGVSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWZ0</accession>
    <name>A0A1U7EWZ0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Peptidase M24 family protein (Homolog to Xaa-Pro dipeptidase)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3076A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323251.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWZ0"/>
    <dbReference type="STRING" id="348780.NP_3076A"/>
    <dbReference type="EnsemblBacteria" id="CAI49629">
        <property type="protein sequence ID" value="CAI49629"/>
        <property type="gene ID" value="NP_3076A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703015"/>
    <dbReference type="KEGG" id="nph:NP_3076A"/>
    <dbReference type="eggNOG" id="arCOG01000">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017266_4_3_2"/>
    <dbReference type="OrthoDB" id="1346at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000587">
        <property type="entry name" value="Creatinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050659">
        <property type="entry name" value="Peptidase_M24B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46112">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46112:SF2">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01321">
        <property type="entry name" value="Creatinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Creatinase N-terminal" evidence="2">
        <location>
            <begin position="5"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M24" evidence="1">
        <location>
            <begin position="152"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="393" mass="42456" checksum="367F24AE34D7F738" modified="2017-05-10" version="1">MNPDLSKLDAYIEEADVDGYLIEADGETSDQRYLSGFTAPDPFVTLYTGETHLLVSALEYGRAQRTARADSVARHTEYDHRENIAEYGPTTGKHRTLAAFLDAHGVESVAVPEACPAGTADGLREQGVVVDVDYDGVVQSIRATKTDAEIDNIRAAQRANEAAMARAESLLEAAAAEDGTLLYDGEPLTSERVKREIEIELLRQDCALDETIVACGRDAADPHDRGSGPLEAGEPIIVDIFPRSKETGYHADMTRTFCVGEPDETVEEWYDLTHEAQQAALDAIEAGASGSEVHAAVCEVYEAAGEPTLRSDPETETGFIHTTGHGVGLDVHESPRVSEQDAELKPGHIITVEPGLYDPTVGGVRIEDLVVVTEDGYENLTDYEKQLVVGDDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX24</accession>
    <name>A0A1U7EX24_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3104A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323265.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX24"/>
    <dbReference type="STRING" id="348780.NP_3104A"/>
    <dbReference type="EnsemblBacteria" id="CAI49643">
        <property type="protein sequence ID" value="CAI49643"/>
        <property type="gene ID" value="NP_3104A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701653"/>
    <dbReference type="KEGG" id="nph:NP_3104A"/>
    <dbReference type="eggNOG" id="arCOG04578">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1656846_0_0_2"/>
    <dbReference type="OrthoDB" id="311801at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043811">
        <property type="entry name" value="DUF5793"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19106">
        <property type="entry name" value="DUF5793"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="150" mass="16327" checksum="0A4F90AE5D6E700B" modified="2017-05-10" version="1">MRRDYFTLSKQGTEADDPATPTLVVGYEGPTEELAERLHDDGTPLEEDGLDVAYRLQEPIDIDDPSGVLAVADRVTGEYVLEVNADAGLVSELVESARDADDPEDGHFRIVIEDEDGTQLDAHKASTLLVYDADGNLLREESLIPSGVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX87</accession>
    <name>A0A1U7EX87_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Acyl-CoA synthetase</fullName>
            <ecNumber evidence="6">6.2.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">acs9</name>
        <name evidence="6" type="ordered locus">NP_3386A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX87"/>
    <dbReference type="STRING" id="348780.NP_3386A"/>
    <dbReference type="EnsemblBacteria" id="CAI49784">
        <property type="protein sequence ID" value="CAI49784"/>
        <property type="gene ID" value="NP_3386A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3386A"/>
    <dbReference type="eggNOG" id="arCOG00856">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016878">
        <property type="term" value="F:acid-thiol ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000008">
        <property type="entry name" value="2,3-dihydroxybenzoate-AMP ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050237">
        <property type="entry name" value="ATP-dep_AMP-bd_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43767">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43767:SF1">
        <property type="entry name" value="NONRIBOSOMAL PEPTIDE SYNTHASE PES1 (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="4">
        <location>
            <begin position="8"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="5">
        <location>
            <begin position="447"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="311"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI49784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="546" mass="59565" checksum="33A06D2B48B823C6" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX95</accession>
    <name>A0A1U7EX95_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3396A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX95"/>
    <dbReference type="STRING" id="348780.NP_3396A"/>
    <dbReference type="EnsemblBacteria" id="CAI49789">
        <property type="protein sequence ID" value="CAI49789"/>
        <property type="gene ID" value="NP_3396A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3396A"/>
    <dbReference type="eggNOG" id="arCOG11368">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1582942_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="168" mass="17716" checksum="3109D5B3F5A7E8BF" modified="2017-05-10" version="1">MFLWSVVRRATELGNKYIDFCALRVIRWCMPSEEMQASERSLTMLASGSAVMHFVAFTAVGYVALESITYGAVAGVLSALGSFFFLPWFIGISSVQSDDGDQIPFAEAAARVPASTQLGTLGGGLEAGSVVMLALGFATDGPNLLYGIAGGLAAALVIHLGWSMLVNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXD1</accession>
    <name>A0A1U7EXD1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3492A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323457.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXD1"/>
    <dbReference type="STRING" id="348780.NP_3492A"/>
    <dbReference type="EnsemblBacteria" id="CAI49837">
        <property type="protein sequence ID" value="CAI49837"/>
        <property type="gene ID" value="NP_3492A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701788"/>
    <dbReference type="KEGG" id="nph:NP_3492A"/>
    <dbReference type="eggNOG" id="arCOG09141">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093042_0_0_2"/>
    <dbReference type="OrthoDB" id="351150at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="213" mass="21826" checksum="170FD1ABDF7B67B0" modified="2017-05-10" version="1">MVPVPLPRLALVVSLAVLGVSLAGFVSAAGLAGGLSTATAGEVTVDDGAVVFADAGGDRTPVADATAAGRIEITDTGGLLAVTTRPKESGALTDSQREAAARIAVSDATLAEQLETSDGVTVDVAPLRADEPVDRQLRRSDIDAGPPAGAVNSTFRSVDTTGSSIALERVGPRYDDRRALVVVEPVDAPDEYRAVVDLNAETVDRILQLEVVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXG2</accession>
    <name>A0A1U7EXG2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Prenyltransferase (Homolog to 1,4-dihydroxy-2-naphthoate octaprenyltransferase)</fullName>
            <ecNumber evidence="7">2.5.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">menA2</name>
        <name evidence="7" type="ordered locus">NP_3556A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.5.1.-" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXG2"/>
    <dbReference type="STRING" id="348780.NP_3556A"/>
    <dbReference type="EnsemblBacteria" id="CAI49869">
        <property type="protein sequence ID" value="CAI49869"/>
        <property type="gene ID" value="NP_3556A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3556A"/>
    <dbReference type="eggNOG" id="arCOG00480">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043611_0_1_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009234">
        <property type="term" value="P:menaquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042371">
        <property type="term" value="P:vitamin K biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13962">
        <property type="entry name" value="PT_UbiA_UBIAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026046">
        <property type="entry name" value="UBIAD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13929">
        <property type="entry name" value="1,4-DIHYDROXY-2-NAPHTHOATE OCTAPRENYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13929:SF0">
        <property type="entry name" value="UBIA PRENYLTRANSFERASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="239"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="263"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="295"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="319" mass="33557" checksum="167957792844FE3F" modified="2017-05-10" version="1">MIRCLHVLYVRHVMLMSTIHHTKRTLQPYFALVRAPFLVFTAIVILLPAAAAAEAGILNVTNTLLAFGSVLAAHVAVNILNLASDYRTGIDEATDETPYSGGNDVLVSGTLSYRRALAAGVIAILASALFVVPLVSQFGGIVLLFYGVGTVLVVGYTDVFARIGLGEFACGAGLTFLPTVAVGYIQAGTFPESVLYLSVPMFLVGFNLLLLNEFPDVEADRTNGRVNIPVVLGRQTAGYIYFGLVGVLVASLLYPIATGALPATVAVAILPIALLAPIFRTVVLEGNGDLTDDELGNHILWTQTTIVALAVGMAAAAVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXM4</accession>
    <name>A0A1U7EXM4_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo4</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit F</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1 2" type="primary">rpoF</name>
        <name evidence="1" type="synonym">rpo4</name>
        <name evidence="2" type="ordered locus">NP_3682A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. This subunit is less well bound than the others.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the RNA polymerase complex. Forms a stalk with Rpo7 that extends from the main structure.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic RPB4 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323550.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXM4"/>
    <dbReference type="STRING" id="348780.NP_3682A"/>
    <dbReference type="EnsemblBacteria" id="CAI49932">
        <property type="protein sequence ID" value="CAI49932"/>
        <property type="gene ID" value="NP_3682A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703157"/>
    <dbReference type="KEGG" id="nph:NP_3682A"/>
    <dbReference type="eggNOG" id="arCOG01016">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165892_0_0_2"/>
    <dbReference type="OrthoDB" id="25158at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HRDC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNApol_arch_Rpo4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
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        <property type="entry name" value="HRDC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005574">
        <property type="entry name" value="Rpb4/RPC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010924">
        <property type="entry name" value="Rpo4"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011550">
        <property type="entry name" value="PRK14981.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39646:SF1">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE SUBUNIT RPO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39646">
        <property type="entry name" value="RNA POLYMERASE RPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03874">
        <property type="entry name" value="RNA_pol_Rpb4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNA_pol_F_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="1 2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0804">Transcription</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="118" mass="13693" checksum="62BB7BC157C9EFFD" modified="2017-05-10" version="1">MTIFKEVLDEEYLTTPEAKELLADIEAERALDEDREMRYELARAIEHVNRFALLDVEEAQALVEDLRELEKVDEATAYKVANLLPEDRDELRTVFAQERYSLSGDELDDILDIVAKYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXN0</accession>
    <name>A0A1U7EXN0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF6199 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3668A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXN0"/>
    <dbReference type="STRING" id="348780.NP_3668A"/>
    <dbReference type="EnsemblBacteria" id="CAI49925">
        <property type="protein sequence ID" value="CAI49925"/>
        <property type="gene ID" value="NP_3668A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701837"/>
    <dbReference type="KEGG" id="nph:NP_3668A"/>
    <dbReference type="eggNOG" id="arCOG07770">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2476111_0_0_2"/>
    <dbReference type="OrthoDB" id="163843at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045679">
        <property type="entry name" value="DUF6199"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19701">
        <property type="entry name" value="DUF6199"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6199" evidence="1">
        <location>
            <begin position="6"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="87" mass="9348" checksum="FCFF8EA9010A3B4A" modified="2017-05-10" version="1">MFWKTLAVAYGVFAAVFPEKKLEYLTRMVLVGYENPEDLEPSDWYVSAVRTEGVLVALAGVGSIVLSLVAASSDTEDAAATGDETDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXP2</accession>
    <name>A0A1U7EXP2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cold shock protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cspA3</name>
        <name evidence="4" type="ordered locus">NP_3734A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323575.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXP2"/>
    <dbReference type="STRING" id="348780.NP_3734A"/>
    <dbReference type="EnsemblBacteria" id="CAI49958">
        <property type="protein sequence ID" value="CAI49958"/>
        <property type="gene ID" value="NP_3734A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702638"/>
    <dbReference type="KEGG" id="nph:NP_3734A"/>
    <dbReference type="eggNOG" id="arCOG02983">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117621_7_2_2"/>
    <dbReference type="OrthoDB" id="202422at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012156">
        <property type="entry name" value="Cold_shock_CspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050181">
        <property type="entry name" value="Cold_shock_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11544">
        <property type="entry name" value="COLD SHOCK DOMAIN CONTAINING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002599">
        <property type="entry name" value="Cold_shock_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00050">
        <property type="entry name" value="COLDSHOCK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CSD" evidence="3">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="64" mass="7006" checksum="872D75B6D1516C32" modified="2017-05-10" version="1">MATGTVDFFNDTGGYGFIETEDADEDVFFHMEDVGGEDLTEGTDLEFDIDEAPKGPRATNVVRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXQ0</accession>
    <name>A0A1U7EXQ0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3730A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323573.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXQ0"/>
    <dbReference type="EnsemblBacteria" id="CAI49956">
        <property type="protein sequence ID" value="CAI49956"/>
        <property type="gene ID" value="NP_3730A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701850"/>
    <dbReference type="KEGG" id="nph:NP_3730A"/>
    <dbReference type="eggNOG" id="arCOG14898">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1709181_0_0_2"/>
    <dbReference type="OrthoDB" id="387330at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="153" mass="15055" checksum="26C7BE1D6CEAD478" modified="2017-05-10" version="1">MTRVRDTAVGLVAVYGGLSLAVGVLAFLGARWAHTYFITAATGDAVAELGPAFVNTVLLQLGVTVLALTPVMSTLAGVLVGRGIFQPVRSIQVAAVGGFVGTLVFGLVVAALGSLGTAAGSLWTTSYLSVLVAASVLAALTGGIGAAVGGRLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXQ4</accession>
    <name>A0A1U7EXQ4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Major facilitator superfamily transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">tp11</name>
        <name evidence="3" type="ordered locus">NP_3798A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323607.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXQ4"/>
    <dbReference type="EnsemblBacteria" id="CAI49990">
        <property type="protein sequence ID" value="CAI49990"/>
        <property type="gene ID" value="NP_3798A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703287"/>
    <dbReference type="KEGG" id="nph:NP_3798A"/>
    <dbReference type="eggNOG" id="arCOG00147">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_59_7_2"/>
    <dbReference type="OrthoDB" id="359492at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF304">
        <property type="entry name" value="MFS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="239"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="266"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="303"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="328"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="369"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="396"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="2">
        <location>
            <begin position="22"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="429" mass="44754" checksum="606174A0FAD5A8BE" modified="2017-05-10" version="1">MGDSSDIDYAGRAREALGFSRWWQIVAAAAMMAAVSPYQYVWSSIEGPLAESLDIALPALGAVFSFYVVFQSLSQLPAGWWRDRRGPRALMFFAALLAGGGYIGLAYATSVWQLYVLYSLGAVGVGIVYTVAVNTAVKWFPDRTGLTTGIGTMAFAGGSALVVPYVRANATVAGYADVLRNVGLVILLVLLVGAVLLRDPPEDWLGLDDRADDNDDGLAASLRGRAYTTGEMLRTWQFWLLYAMFVATAGADLLLIANVVRMAEALGLAAVVATAAATLLPIAAGVSRLVLGAVSDRFDRVHVMAVSFVLAGAFRLALVPAGVADAGIAFVAFVLGAMFFSSPLYVYFPAMVADYYGAEHSSSNYAALYTAKVGGGVFAGTVAGALVAAFGWVPTLTLGGALAVAAGLAALLLRPPTDAGLERDDATAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXR4</accession>
    <name>A0A1U7EXR4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cupin 2 barrel domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3822A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323619.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXR4"/>
    <dbReference type="STRING" id="348780.NP_3822A"/>
    <dbReference type="EnsemblBacteria" id="CAI50002">
        <property type="protein sequence ID" value="CAI50002"/>
        <property type="gene ID" value="NP_3822A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701874"/>
    <dbReference type="KEGG" id="nph:NP_3822A"/>
    <dbReference type="eggNOG" id="arCOG02998">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126380_0_0_2"/>
    <dbReference type="OrthoDB" id="190812at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051610">
        <property type="entry name" value="GPI/OXD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35848">
        <property type="entry name" value="OXALATE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35848:SF9">
        <property type="entry name" value="SLL1358 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin type-2" evidence="2">
        <location>
            <begin position="38"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="167" mass="18024" checksum="04533928D9957FD3" modified="2017-05-10" version="1">MRKVAIDEVDIENSPLGVHSVRRPVSDALGTEHFAMNYFELQPGESFSGGLHTHHDQEEVFYVQSGEATFEVTDEPDSSTTEAVTAGAGEVIRFPPGEFQEGYNDAENDDPVVGFALGAPSSKHDWEAIESVMHCGDCGEETGHSMSLTDEGSFSFTCGECGNEIAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXW2</accession>
    <name>A0A1U7EXW2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Integrase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3892A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323654.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXW2"/>
    <dbReference type="STRING" id="348780.NP_3892A"/>
    <dbReference type="EnsemblBacteria" id="CAI50037">
        <property type="protein sequence ID" value="CAI50037"/>
        <property type="gene ID" value="NP_3892A"/>
    </dbReference>
    <dbReference type="GeneID" id="32155017"/>
    <dbReference type="KEGG" id="nph:NP_3892A"/>
    <dbReference type="eggNOG" id="arCOG06451">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_926275_0_0_2"/>
    <dbReference type="OrthoDB" id="142231at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="300" mass="33800" checksum="671CF2CB66A213A4" modified="2017-05-10" version="1">MNGDPLTREQTAAFRQAADQGDFVDRLLMVLLHTGLTATEFTHMHDNWLEPLPESVEAYQGDTPIIRIPTEADCRGIKLGTNLEIHQRTGRCGYCNDLGYEVWHPGQPDSDCRVRSIPIPDETTARTLYWWFARYDSTPISVNSLERRLSKLADRADIGRDVTSRDVRATYGTNLVRMGFSSEEIAKLMGLFHHISTREFFEAADEPVDWPEVVSDATILAAIAENEPVIKSDVGDLVGFSVTAGRKRANDLIDRGILKKVGERHEGHVHGTTYGIDPSVLKDEGYHEIAERYYKARLEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXW5</accession>
    <name>A0A1U7EXW5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC-type transport system periplasmic substrate-binding protein (Probable substrate copper)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">nosD1</name>
        <name evidence="3" type="synonym">abc11s</name>
        <name evidence="3" type="ordered locus">NP_3916A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323666.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXW5"/>
    <dbReference type="STRING" id="348780.NP_3916A"/>
    <dbReference type="EnsemblBacteria" id="CAI50049">
        <property type="protein sequence ID" value="CAI50049"/>
        <property type="gene ID" value="NP_3916A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702404"/>
    <dbReference type="KEGG" id="nph:NP_3916A"/>
    <dbReference type="eggNOG" id="arCOG02519">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_426783_0_0_2"/>
    <dbReference type="OrthoDB" id="29186at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008719">
        <property type="entry name" value="N2O_reductase_NosL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007742">
        <property type="entry name" value="NosD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051550">
        <property type="entry name" value="SCF-Subunits/Alg-Epimerases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22990">
        <property type="entry name" value="F-BOX ONLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22990:SF15">
        <property type="entry name" value="F-BOX ONLY PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05048">
        <property type="entry name" value="NosD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05573">
        <property type="entry name" value="NosL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160387">
        <property type="entry name" value="NosL/MerB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Periplasmic copper-binding protein NosD beta helix" evidence="2">
        <location>
            <begin position="374"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="626" mass="66124" checksum="BEB5CBC3E86FB3AD" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXW7</accession>
    <name>A0A1U7EXW7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Molybdate/tungstate import ATP-binding protein WtpC</fullName>
            <ecNumber evidence="13">7.3.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">abc13a</name>
        <name evidence="18" type="ordered locus">NP_3932A</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Part of the ABC transporter complex WtpABC involved in molybdate/tungstate import. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>tungstate(in) + ATP + H2O = tungstate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:35027"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46502"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.3.2.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">The complex is composed of two ATP-binding proteins (WtpC), two transmembrane proteins (WtpB) and a solute-binding protein (WtpA).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the ABC transporter superfamily. Sulfate/tungstate importer (TC 3.A.1.6) family.</text>
    </comment>
    <dbReference type="EC" id="7.3.2.6" evidence="13"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323674.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXW7"/>
    <dbReference type="STRING" id="348780.NP_3932A"/>
    <dbReference type="EnsemblBacteria" id="CAI50057">
        <property type="protein sequence ID" value="CAI50057"/>
        <property type="gene ID" value="NP_3932A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702408"/>
    <dbReference type="KEGG" id="nph:NP_3932A"/>
    <dbReference type="eggNOG" id="arCOG00177">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_2"/>
    <dbReference type="OrthoDB" id="18368at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015408">
        <property type="term" value="F:ABC-type ferric iron transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901238">
        <property type="term" value="F:ABC-type tungstate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03259">
        <property type="entry name" value="ABC_Carb_Solutes_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000425">
        <property type="entry name" value="Probable ABC transporter, ATP-binding subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050093">
        <property type="entry name" value="ABC_SmlMolc_Importer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015853">
        <property type="entry name" value="ABC_transpr_FbpC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013611">
        <property type="entry name" value="Transp-assoc_OB_typ2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42781">
        <property type="entry name" value="SPERMIDINE/PUTRESCINE IMPORT ATP-BINDING PROTEIN POTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42781:SF4">
        <property type="entry name" value="SPERMIDINE_PUTRESCINE IMPORT ATP-BINDING PROTEIN POTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08402">
        <property type="entry name" value="TOBE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="MOP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0410">Iron transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="17">
        <location>
            <begin position="3"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00041133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00057369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAI50057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="367" mass="40043" checksum="DB3E5AB6385C0B3D" modified="2017-05-10" version="1">MTLELDGVSYRYGDTDVLASVDLRLDDGESAAVLGPSGCGKTTLVQLVAGHLRPTAGRIRLDGDDVTADPPEERDVGVVFQESTLFPHLTVAENVGYGLTAADVPDDRRQRRVDELLELVGLDDRRDAAPSSLSGGEKRRIELARALAPRPDVLVLDEPLSALDRQLKGRLRRDVDRLRRETDVTMLVVTHDQPTATALSDRLAVLHEGQFTGVGTPTALREDPPSAFVASFVGRENVVEATVRSRQPPELSVCGRRLRLAELPDECGSEEEEEEVACCIPPDAIELTVDGDEPAEERPTALRLQGAVDHVAELGREYSVFVRLQNDETLRVEQRQSPPVGASVRLAVERSAIRLFADLPTSPCQGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXX8</accession>
    <name>A0A1U7EXX8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">DASS family transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">tp27</name>
        <name evidence="9" type="ordered locus">NP_3906A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXX8"/>
    <dbReference type="STRING" id="348780.NP_3906A"/>
    <dbReference type="EnsemblBacteria" id="CAI50044">
        <property type="protein sequence ID" value="CAI50044"/>
        <property type="gene ID" value="NP_3906A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3906A"/>
    <dbReference type="eggNOG" id="arCOG00237">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005170_6_1_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="entry name" value="Regulator of K+ conductance, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004680">
        <property type="entry name" value="Cit_transptr-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051679">
        <property type="entry name" value="DASS-Related_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43652">
        <property type="entry name" value="BASIC AMINO ACID ANTIPORTER YFCC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43652:SF2">
        <property type="entry name" value="BASIC AMINO ACID ANTIPORTER YFCC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03600">
        <property type="entry name" value="CitMHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="TrkA C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="430"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="474"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="505"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="532"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="558"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="598"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="8">
        <location>
            <begin position="223"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="8">
        <location>
            <begin position="318"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI50044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="620" mass="65905" checksum="8CD6AA3971751534" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY67</accession>
    <name>A0A1U7EY67_NATPD</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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        <name evidence="2" type="ordered locus">NP_4108A</name>
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    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323761.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY67"/>
    <dbReference type="STRING" id="348780.NP_4108A"/>
    <dbReference type="EnsemblBacteria" id="CAI50145">
        <property type="protein sequence ID" value="CAI50145"/>
        <property type="gene ID" value="NP_4108A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701975"/>
    <dbReference type="KEGG" id="nph:NP_4108A"/>
    <dbReference type="eggNOG" id="ENOG502N5EK">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1754756_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="148" mass="13970" checksum="9E387FE58E7816C7" modified="2017-05-10" version="1">MVSFPAGDTLAAYATGERPRAATGVVVAVAAVAAVSLAALGAMEAAFGDAPSGAVFMFVVGPLPVAAGAVCGWFRLGFPAAAVSGVSTGVAFYVVVAVGAAVDVGGFGGGDTPLAAFSMVLASTGLVWATGGFVAGVIANLATGGAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY76</accession>
    <name>A0A1U7EY76_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ABC-type transport system permease protein (Probable substrate branched-chain amino acids)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">livH1</name>
        <name evidence="11" type="synonym">abc05p1</name>
        <name evidence="11" type="ordered locus">NP_4132A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323773.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY76"/>
    <dbReference type="STRING" id="348780.NP_4132A"/>
    <dbReference type="EnsemblBacteria" id="CAI50157">
        <property type="protein sequence ID" value="CAI50157"/>
        <property type="gene ID" value="NP_4132A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702379"/>
    <dbReference type="KEGG" id="nph:NP_4132A"/>
    <dbReference type="eggNOG" id="arCOG01269">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039929_1_0_2"/>
    <dbReference type="OrthoDB" id="31233at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015188">
        <property type="term" value="F:L-isoleucine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015190">
        <property type="term" value="F:L-leucine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015192">
        <property type="term" value="F:L-phenylalanine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005304">
        <property type="term" value="F:L-valine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042941">
        <property type="term" value="P:D-alanine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015808">
        <property type="term" value="P:L-alanine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903806">
        <property type="term" value="P:L-isoleucine import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06582">
        <property type="entry name" value="TM_PBP1_LivH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052157">
        <property type="entry name" value="BCAA_transport_permease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11795">
        <property type="entry name" value="BRANCHED-CHAIN AMINO ACID TRANSPORT SYSTEM PERMEASE PROTEIN LIVH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11795:SF371">
        <property type="entry name" value="HIGH-AFFINITY BRANCHED-CHAIN AMINO ACID TRANSPORT SYSTEM PERMEASE PROTEIN LIVH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="4" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="61"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="216"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="275"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="303"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="346"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI50157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="373" mass="38830" checksum="67CA17D3CEE93F14" modified="2017-05-10" version="1">MDVANSTLAASIRERPGTALVGLFGVLLAVDLVAKLAGVQLGPIGGRLSVGRFTSGLTQGIVIGVIIGLAGIGLSMTYSILNFANFSHGDLITVGAFSGWGVAYLILGWGIVATDPEYGIGTLLLVRRPGVGFTELGASVLLSPWAILGGLVAAAVITALLALAIDRVVYKPMRDRSGISLLIVSIGVALVLRYGIQFIYGPGTRPVTSSERTLELGVASIDTHLATIVVVGVGLIIGTHYLLQQTKLGTAMRAMSDNKDLALVTGIPTERTITWTWLIGGGLAGASGFLYILIQTLQFDRGWLLLLLIFAAVILGGIGSIYGAIAGGLVIGLVFQLSPIWVGSDFNYVAAYAIMILVLLLRPEGLFKGVSTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY95</accession>
    <name>A0A1U7EY95_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4180A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323797.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY95"/>
    <dbReference type="STRING" id="348780.NP_4180A"/>
    <dbReference type="EnsemblBacteria" id="CAI50181">
        <property type="protein sequence ID" value="CAI50181"/>
        <property type="gene ID" value="NP_4180A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701995"/>
    <dbReference type="KEGG" id="nph:NP_4180A"/>
    <dbReference type="eggNOG" id="arCOG04608">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1773249_0_0_2"/>
    <dbReference type="OrthoDB" id="156190at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043814">
        <property type="entry name" value="DUF5796"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19109">
        <property type="entry name" value="DUF5796"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="145" mass="15583" checksum="E71BEC3946FCFD24" modified="2017-05-10" version="1">MTSRSDIAPETLTVSLESEGVEVEYLDGRSVFYHGVPEKKAGAVHCPPGKDVHVLVTAPDETQGVMVYVNDRNTHDDILESTGVGRVIIEGGETASLFPGVAAESTGYRVEVSADFDVADGRVFVFAEDQMGEFSYELVPESEVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYC9</accession>
    <name>A0A1U7EYC9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RNA-binding protein Lsm</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">lsm</name>
        <name evidence="2" type="ordered locus">NP_4308A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323861.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYC9"/>
    <dbReference type="STRING" id="348780.NP_4308A"/>
    <dbReference type="EnsemblBacteria" id="CAI50245">
        <property type="protein sequence ID" value="CAI50245"/>
        <property type="gene ID" value="NP_4308A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702027"/>
    <dbReference type="KEGG" id="nph:NP_4308A"/>
    <dbReference type="eggNOG" id="arCOG00998">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076902_11_1_2"/>
    <dbReference type="OrthoDB" id="371816at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sm" evidence="1">
        <location>
            <begin position="4"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="61" mass="6656" checksum="EF18DC33BD3CC7BC" modified="2017-05-10" version="1">MSNRPLDVLEETLGAEVHVTLKGGETYEGTLSGYDQHMNLVLEEGDNVTIIRGDNVVSIEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYG6</accession>
    <name>A0A1U7EYG6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4338A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYG6"/>
    <dbReference type="EnsemblBacteria" id="CAI50260">
        <property type="protein sequence ID" value="CAI50260"/>
        <property type="gene ID" value="NP_4338A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_4338A"/>
    <dbReference type="eggNOG" id="arCOG06190">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_152990_1_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055894">
        <property type="entry name" value="DUF7471"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24283">
        <property type="entry name" value="DUF7471"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="112" mass="11916" checksum="B1B9057C79F18C1B" modified="2017-05-10" version="1">MNGLKSHLGSWLGPELAPILLCAIALAILGTMLLFALGVAAYLRTRSDRYLLLAAATGALVARSLLGLGTVFDIVPMFVHHLVAHSLDFLVALTILVAAYLHRGSDNYSTNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYJ0</accession>
    <name>A0A1U7EYJ0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Non-canonical purine NTP pyrophosphatase</fullName>
            <ecNumber evidence="3">3.6.1.66</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ham1</name>
        <name evidence="3" type="ordered locus">NP_4426A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HAM1 NTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.66" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323919.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYJ0"/>
    <dbReference type="STRING" id="348780.NP_4426A"/>
    <dbReference type="EnsemblBacteria" id="CAI50304">
        <property type="protein sequence ID" value="CAI50304"/>
        <property type="gene ID" value="NP_4426A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702791"/>
    <dbReference type="KEGG" id="nph:NP_4426A"/>
    <dbReference type="eggNOG" id="arCOG04184">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082080_1_0_2"/>
    <dbReference type="OrthoDB" id="372108at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036220">
        <property type="term" value="F:ITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009143">
        <property type="term" value="P:nucleoside triphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00515">
        <property type="entry name" value="HAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002637">
        <property type="entry name" value="RdgB/HAM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067:SF9">
        <property type="entry name" value="INOSINE TRIPHOSPHATE PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067">
        <property type="entry name" value="INOSINE TRIPHOSPHATE PYROPHOSPHATASE/HAM1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01725">
        <property type="entry name" value="Ham1p_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="ITPase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="214" mass="23407" checksum="4C6A5A756258DE96" modified="2017-05-10" version="1">MLRYVTTNDGKVREAEAYLDDDVAAFEYDYQEIQADSLEAVAADGARKAYRHVGEPVIVDDAGLYLEGFDGFPGPYSAYVENTLGVECVGRLARREEATQAKFRCVIAYCDGDSFEASPEPVDTDDRRGTDIDADERATAATDETVAGEELPVKLFPAAVPGHIVEPRGDGGFGYDPIFEHDGTTFAEMTPEEKNAVSHRGRALSTFAEWYAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYM4</accession>
    <name>A0A1U7EYM4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4510A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323961.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYM4"/>
    <dbReference type="STRING" id="348780.NP_4510A"/>
    <dbReference type="EnsemblBacteria" id="CAI50346">
        <property type="protein sequence ID" value="CAI50346"/>
        <property type="gene ID" value="NP_4510A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702083"/>
    <dbReference type="KEGG" id="nph:NP_4510A"/>
    <dbReference type="eggNOG" id="arCOG04775">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1514566_0_0_2"/>
    <dbReference type="OrthoDB" id="156206at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043853">
        <property type="entry name" value="DUF5815"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19132">
        <property type="entry name" value="DUF5815"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="177" mass="19786" checksum="B6052823E569223D" modified="2017-05-10" version="1">MPTPRVPGDDGTDRLELPCGESIHPHDVDMGLRELSCDCGETHAVVVDVHPVSRFVPEETVDVLEATIETDDDFPTFGMAHVMGMVLEEFPDRVAVADCADDGVVGYALLWVTDFDARRLHEIVVELLVELMDHAVGHADDETAAEFQQQLSAFDVETFVEEYRREREFETEADRPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYM7</accession>
    <name>A0A1U7EYM7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">CRM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_4478A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323945.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYM7"/>
    <dbReference type="STRING" id="348780.NP_4478A"/>
    <dbReference type="EnsemblBacteria" id="CAI50330">
        <property type="protein sequence ID" value="CAI50330"/>
        <property type="gene ID" value="NP_4478A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702074"/>
    <dbReference type="KEGG" id="nph:NP_4478A"/>
    <dbReference type="eggNOG" id="arCOG01346">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095994_5_2_2"/>
    <dbReference type="OrthoDB" id="30785at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.60">
        <property type="entry name" value="YhbY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001890">
        <property type="entry name" value="RNA-binding_CRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051925">
        <property type="entry name" value="RNA-binding_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035920">
        <property type="entry name" value="YhbY-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40065">
        <property type="entry name" value="RNA-BINDING PROTEIN YHBY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40065:SF3">
        <property type="entry name" value="RNA-BINDING PROTEIN YHBY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01985">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01103">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75471">
        <property type="entry name" value="YhbY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51295">
        <property type="entry name" value="CRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="CRM" evidence="3">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="82" mass="8968" checksum="01DE6FAB7B4AD298" modified="2017-05-10" version="1">MNDRDLQARAHDIDATLRVGKAGIDAVVDELSDQLADRDLVKVKFLRAARGGTTTEELAADLADRVNADLVETRGHTAVFHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYS0</accession>
    <name>A0A1U7EYS0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">IS1341-type transposase ISNph19</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_4572A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323992.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYS0"/>
    <dbReference type="STRING" id="348780.NP_4572A"/>
    <dbReference type="EnsemblBacteria" id="CAI50377">
        <property type="protein sequence ID" value="CAI50377"/>
        <property type="gene ID" value="NP_4572A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702101"/>
    <dbReference type="KEGG" id="nph:NP_4572A"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_3_3_2"/>
    <dbReference type="OrthoDB" id="210698at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF26">
        <property type="entry name" value="TRANSPOSASE, PROBABLY IS605-TNPB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas12f1-like TNB" evidence="3">
        <location>
            <begin position="301"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="399"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="402"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="420" mass="47382" checksum="17202E4529226946" modified="2017-05-10" version="1">MAVVRNLEIKLDVDEDAHSVLDETFEQFRQAAQHAADYGWSDDPTQIIDSKSKLHDATYSDVREKTDLTANHVQAARSLAANALENCKDRILEDGKKASKPEFRGTVVFYDGRTITYNDDHCTLSTTEDRVTAEYIVPEDTRGTPFEEYWENDEWERGESTLHKRDGTYYLHVSMKKEREEDEASTSEYGAVLGVDLNVDGYLAVTSTGAFLGNADYLNHKRAEYERRRGKLQQTGTRSAHLTLKSIGDRFSNWSEDYLHRVALAVVLEARRHDCDAIAFENLKHIRGRISNASKFQQWAFNKLQSLVEYKAEEYGILVDDVKPQYTSQRCSHSKCGFTHEDNRDGDEFECLKCGKELHADYNAARNVGWRLVQHWLKSGAGRADCQVALKSGMVNANGEFSPTTGSSGQSGSPLTSPPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYS4</accession>
    <name>A0A1U7EYS4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4598A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324005.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYS4"/>
    <dbReference type="STRING" id="348780.NP_4598A"/>
    <dbReference type="EnsemblBacteria" id="CAI50390">
        <property type="protein sequence ID" value="CAI50390"/>
        <property type="gene ID" value="NP_4598A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702109"/>
    <dbReference type="KEGG" id="nph:NP_4598A"/>
    <dbReference type="HOGENOM" id="CLU_2949405_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058293">
        <property type="entry name" value="DUF7987"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25949">
        <property type="entry name" value="DUF7987"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="59" mass="6205" checksum="FE95D4A3D0B10C4D" modified="2017-05-10" version="1">MAPSKTLVTAVCVILAAPFSYAAYQQWSLVVAAAVLVGIGIVLPRLYAFIAVPHSSDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYV1</accession>
    <name>A0A1U7EYV1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HTH domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4670A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324040.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYV1"/>
    <dbReference type="STRING" id="348780.NP_4670A"/>
    <dbReference type="EnsemblBacteria" id="CAI50426">
        <property type="protein sequence ID" value="CAI50426"/>
        <property type="gene ID" value="NP_4670A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702137"/>
    <dbReference type="KEGG" id="nph:NP_4670A"/>
    <dbReference type="eggNOG" id="arCOG02756">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130787_0_0_2"/>
    <dbReference type="OrthoDB" id="325082at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25212">
        <property type="entry name" value="HVO_A0114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="149" mass="16953" checksum="7FD27BCD3E12B7F7" modified="2017-05-10" version="1">MSKNTPDNPHEPPEDAEYPSTLRITSKPFADHKESVLDRAERWEQGEEVPHVVNFQDASRLQRILTSRRLELVQSLMGSPAESMRALADRLGRDVRQVHDDLQILNEYRIVHFREEGGAKKPHVPYDTVKIEVELTKSLDDASESPASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ00</accession>
    <name>A0A1U7EZ00_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Carotenoid 3,4-desaturase</fullName>
            <ecNumber evidence="5">1.3.99.37</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">crtD</name>
        <name evidence="5" type="synonym">crtI1</name>
        <name evidence="5" type="ordered locus">NP_4764A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carotenoid biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="1.3.99.37" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324085.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ00"/>
    <dbReference type="STRING" id="348780.NP_4764A"/>
    <dbReference type="EnsemblBacteria" id="CAI50473">
        <property type="protein sequence ID" value="CAI50473"/>
        <property type="gene ID" value="NP_4764A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702633"/>
    <dbReference type="KEGG" id="nph:NP_4764A"/>
    <dbReference type="eggNOG" id="arCOG01521">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019722_2_1_2"/>
    <dbReference type="OrthoDB" id="40741at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016117">
        <property type="term" value="P:carotenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014105">
        <property type="entry name" value="Carotenoid/retinoid_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02734">
        <property type="entry name" value="crtI_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43734">
        <property type="entry name" value="PHYTOENE DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43734:SF1">
        <property type="entry name" value="PHYTOENE DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0125">Carotenoid biosynthesis</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="4">
        <location>
            <begin position="15"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI50473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="492" mass="55226" checksum="9C97CB7EDE77D3FC" modified="2017-05-10" version="1">MSSTRSVVVVGSGFGGLSAACYLADAGLDVTVLEKNEQLGGRASVLETEGFRFDMGPSWYLMPDVFERFFDHFDREPGDFYKLERLDPHYRIFWKDGDTAAVTDDREEMRKLFEEYESGAGEALDSYLATSRDHYEFGMETFVYEDRPRLRDWVALDVMRAAPVGLKLLGTMDDYVADYFDDPKLRQIMQYSLVFLGGAPNNTPAIYNMMSHVDFNLGVYYPENGLRSVVDACVELGEELGVEYVTDAEVTEIVNRKDGFTVESSAGDFDADRVVSNADYAHTEQELLPEHERQYDAEYWDSRTYSPSAFLLYLGVEGDVEPLEHHTLVLPTDWDGHFEQIFDTPAWPDDPAYYLCVPSKTDDSVAPEGHSNLFALVPVAPGLDDDAETRAWYRDLVLDDIAENTGVDLHDRIVFEETFTVSEFADRYNSTQGTALGLAHTLRQTALLRPPHRSDACPGLYFTGAFTTPGIGVPMCLISGEHTAEALLNDAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ34</accession>
    <name>A0A1U7EZ34_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">Iron-sulfur cluster carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">apbC2</name>
        <name evidence="11" type="ordered locus">NP_4940A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 8">Binds and transfers iron-sulfur (Fe-S) clusters to target apoproteins. Can hydrolyze ATP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the Mrp/NBP35 ATP-binding proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324173.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ34"/>
    <dbReference type="STRING" id="348780.NP_4940A"/>
    <dbReference type="EnsemblBacteria" id="CAI50561">
        <property type="protein sequence ID" value="CAI50561"/>
        <property type="gene ID" value="NP_4940A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702937"/>
    <dbReference type="KEGG" id="nph:NP_4940A"/>
    <dbReference type="eggNOG" id="arCOG00585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG01846">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024839_0_0_2"/>
    <dbReference type="OrthoDB" id="8297at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02037">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001119">
        <property type="entry name" value="Iron-sulfur cluster carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000808">
        <property type="entry name" value="Mrp-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044304">
        <property type="entry name" value="NUBPL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42961">
        <property type="entry name" value="IRON-SULFUR PROTEIN NUBPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42961:SF2">
        <property type="entry name" value="IRON-SULFUR PROTEIN NUBPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01215">
        <property type="entry name" value="MRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0408">Iron</keyword>
    <keyword evidence="5 8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIP18 family-like" evidence="10">
        <location>
            <begin position="5"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="350"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="350"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="363"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="101"/>
            <end position="108"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00074706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI50561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="372" mass="39859" checksum="EB2BB8BF43B7FCF7" modified="2017-05-10" version="1">MTELEDELEARLREIEDPIVGEDILSMQLINDVEIDDGTASISLAFNTPFAPAELELGDEIRAAVSDVGLEPDLYAEVGREHGFDEEVMPNVRNVVAVASGKGGVGKTTVAANLAAGLDELGARVGLLDADIHGPNAPRVLPVEEQPGVTPDEKIVPPTADGVKVMSMGFLLEEEDDPAILRGPMVNNVMTHFFENVEWGALDYLVVDLPPGTGDASLDLVQTLPVAGVVIVTTPQEMAVDDARKGLRLFEKHETPVLGIVENMSRYHCPSCGDEHDPFGRGGAEEMVESYDVELLGQLPIHEDFGADGSELPAVKLDASPVQDAAQSVMTDIADRLGEVNRLKVADMLELGREEDKSPVDESPQSQSGPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ58</accession>
    <name>A0A1U7EZ58_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4922A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324164.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ58"/>
    <dbReference type="EnsemblBacteria" id="CAI50552">
        <property type="protein sequence ID" value="CAI50552"/>
        <property type="gene ID" value="NP_4922A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702201"/>
    <dbReference type="KEGG" id="nph:NP_4922A"/>
    <dbReference type="eggNOG" id="arCOG06440">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155007_0_0_2"/>
    <dbReference type="OrthoDB" id="211056at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058375">
        <property type="entry name" value="DUF8062"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26258">
        <property type="entry name" value="DUF8062"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="115" mass="12982" checksum="A8D51CFE0E8828FD" modified="2017-05-10" version="1">MADSFDSDTRPPDDVDDTPTVSCSRCGDEWDLDYELDTLRVGNQAVERFALDHKRHTGHFPDDVTPWVAACEQCPDGDEFLRERPAQRWAEIHARHTGHAVSLKGSDDETATVDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ75</accession>
    <name>A0A1U7EZ75_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">TIGR01210 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">NP_5012A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324207.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ75"/>
    <dbReference type="STRING" id="348780.NP_5012A"/>
    <dbReference type="EnsemblBacteria" id="CAI50597">
        <property type="protein sequence ID" value="CAI50597"/>
        <property type="gene ID" value="NP_5012A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702227"/>
    <dbReference type="KEGG" id="nph:NP_5012A"/>
    <dbReference type="eggNOG" id="arCOG01360">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060488_0_0_2"/>
    <dbReference type="OrthoDB" id="105445at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002926">
        <property type="term" value="P:tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039661">
        <property type="entry name" value="ELP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005909">
        <property type="entry name" value="RaSEA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01210">
        <property type="entry name" value="archaeosine biosynthesis radical SAM protein RaSEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135:SF0">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004954">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Elp3/MiaA/NifB-like radical SAM core" evidence="7">
        <location>
            <begin position="55"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI50597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="358" mass="39877" checksum="B1D938BCD3BA62BD" modified="2017-05-10" version="1">MSQPTPDTYEDGRGMDAHNEVMREIRARNDDTYDPTEPTRVWLDEDNTPDGVYQSLTIILNTGGCRWARAGGCTMCGYVAESVEGGSVGHENLMTQIQHCLDHEADNADEKAGLIKIYTSGSFLDEREIPAETRAAIAETFADRDRMVVESLPDFVDREKVADFTDRGLETDVAIGLETATDRVRHDAVNKYFDFAEFEDACVEAQATGAGVKAYLLLKPPFLAEPDAVEDMKRSVRRCAAADGCHTVSMNPCNVQRHTMVEELYHDGGYRPPWLWSVCDVLEATADEDVIVVSDPVGHGSDRGPHNCGECDDLVQRAIKDFNLRQDPSVFEEVDCECTATWEYVMENETAYNQPLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ83</accession>
    <name>A0A1U7EZ83_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF1628 domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5032A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324217.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ83"/>
    <dbReference type="STRING" id="348780.NP_5032A"/>
    <dbReference type="EnsemblBacteria" id="CAI50607">
        <property type="protein sequence ID" value="CAI50607"/>
        <property type="gene ID" value="NP_5032A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702235"/>
    <dbReference type="KEGG" id="nph:NP_5032A"/>
    <dbReference type="eggNOG" id="arCOG02425">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1631701_0_0_2"/>
    <dbReference type="OrthoDB" id="201989at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013373">
        <property type="entry name" value="Flagellin/pilin_N_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012859">
        <property type="entry name" value="Pilin_N_archaeal"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02537">
        <property type="entry name" value="arch_flag_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07790">
        <property type="entry name" value="Pilin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Archaeal Type IV pilin N-terminal" evidence="1">
        <location>
            <begin position="4"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="154" mass="15753" checksum="A106C41427494A45" modified="2017-05-10" version="1">MSDRAVAPIVAVVCLVGVVVALAAAVGGAVPTDVAAPPPSATIDASAGADGELRLTHRGGEPLTPETLRLRVSVDGERLDKQPPVPFFSAHGFESGPDGPFNSAWNGDWRAGETATVTVAETNAPSVTVGSTVEIRLYAGDHRLARLETTASTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ87</accession>
    <name>A0A1U7EZ87_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Abi/CAAX domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_4990A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324197.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ87"/>
    <dbReference type="STRING" id="348780.NP_4990A"/>
    <dbReference type="EnsemblBacteria" id="CAI50586">
        <property type="protein sequence ID" value="CAI50586"/>
        <property type="gene ID" value="NP_4990A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702224"/>
    <dbReference type="KEGG" id="nph:NP_4990A"/>
    <dbReference type="eggNOG" id="arCOG02766">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073769_0_0_2"/>
    <dbReference type="OrthoDB" id="275779at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080120">
        <property type="term" value="P:CAAX-box protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1/LyrA-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39430">
        <property type="entry name" value="MEMBRANE-ASSOCIATED PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39430:SF1">
        <property type="entry name" value="PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="CAAX prenyl protease 2/Lysostaphin resistance protein A-like" evidence="2">
        <location>
            <begin position="136"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="248" mass="26264" checksum="DADA72E25ADF0D18" modified="2017-05-10" version="1">MDRRYGGRPKWRVVADMTAVGAGALIVGMLTGIVVFWLLGQPLGLDPEAPAGQLVFSLGTYSGLAAAGLLYLVRHELPLSYVRARLPSVRDIVATVVTLGLLGVLAIALPEVVDRLGLPLAEHGVADVIEVNPAVALAFVPLSILVVGPAEEFVYRGIIQTRLRERFDVVNAVAIASVVFAVVHVLAYLDPANPLGTLVTVVFLLLPLGAVLGAAYEYTENLVVVAVAHGVYNATVFMVSYADVVGMW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZA6</accession>
    <name>A0A1U7EZA6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Proteasome-activating nucleotidase</fullName>
            <shortName evidence="10">PAN</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Proteasomal ATPase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Proteasome regulatory ATPase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Proteasome regulatory particle</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">pan1</name>
        <name evidence="10" type="synonym">pan</name>
        <name evidence="13" type="ordered locus">NP_5038A</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">ATPase which is responsible for recognizing, binding, unfolding and translocation of substrate proteins into the archaeal 20S proteasome core particle. Is essential for opening the gate of the 20S proteasome via an interaction with its C-terminus, thereby allowing substrate entry and access to the site of proteolysis. Thus, the C-termini of the proteasomal ATPase function like a 'key in a lock' to induce gate opening and therefore regulate proteolysis. Unfolding activity requires energy from ATP hydrolysis, whereas ATP binding alone promotes ATPase-20S proteasome association which triggers gate opening, and supports translocation of unfolded substrates.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homohexamer. The hexameric complex has a two-ring architecture resembling a top hat that caps the 20S proteasome core at one or both ends. Upon ATP-binding, the C-terminus of PAN interacts with the alpha-rings of the proteasome core by binding to the intersubunit pockets.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">Consists of three main regions, an N-terminal coiled-coil domain that may assist in substrate recognition, an interdomain involved in PAN hexamerization, and a C-terminal ATPase domain of the AAA type.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10 11">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324220.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZA6"/>
    <dbReference type="STRING" id="348780.NP_5038A"/>
    <dbReference type="EnsemblBacteria" id="CAI50610">
        <property type="protein sequence ID" value="CAI50610"/>
        <property type="gene ID" value="NP_5038A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703007"/>
    <dbReference type="KEGG" id="nph:NP_5038A"/>
    <dbReference type="eggNOG" id="arCOG01306">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_2_2_2"/>
    <dbReference type="OrthoDB" id="77269at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022623">
        <property type="term" value="C:proteasome-activating nucleotidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043335">
        <property type="term" value="P:protein unfolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000033">
        <property type="entry name" value="26S protease regulatory subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000001">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00553">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023501">
        <property type="entry name" value="Nucleotidase_PAN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003069">
        <property type="entry name" value="PRK03992.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF047747">
        <property type="entry name" value="PrtsmActNtasePan2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10 13" id="KW-0647">Proteasome</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="12">
        <location>
            <begin position="184"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="39"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="195"/>
            <end position="200"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAI50610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="405" mass="44758" checksum="B467AE711989F08C" modified="2017-05-10" version="1">MSHSPSLPDRPTLNLDPEMSEAERLAALRNHFIEVSQVHEELADQLEDARERQSELHEEVSELQAENEALKTSSLYLATVEELTDDQVLLKQHGNNQEVITDVSPRLYNQLEAGDRVAINDSFAIQTVLDAETDARAQAMEISERPDVGYADIGGLDEQIREVREAVEDPLTNPGKFEEVGIEPPTGVLLHGPPGTGKTMLAKAVANQTDATFIKMAGSELVQKFIGEGARLVRDLFELAAEREPAIIFIDEIDAIAAKRTESKTSGDAEVQRTMMQLLSEMDGFDRRGDISIIAATNRFDMLDRAILRPGRFDRLIEVPEPDIEGRTQILQIHSRGMQLDSAIDLAAIAEETEGFSGAELESLTTEAGMFAIRDGRTEVRREDFDDALEKIAEDDAAGTPIAFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZE8</accession>
    <name>A0A1U7EZE8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UspA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_5138A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324270.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZE8"/>
    <dbReference type="STRING" id="348780.NP_5138A"/>
    <dbReference type="EnsemblBacteria" id="CAI50660">
        <property type="protein sequence ID" value="CAI50660"/>
        <property type="gene ID" value="NP_5138A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702268"/>
    <dbReference type="KEGG" id="nph:NP_5138A"/>
    <dbReference type="eggNOG" id="arCOG02053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_11_1_2"/>
    <dbReference type="OrthoDB" id="107030at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF6">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN UP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006276">
        <property type="entry name" value="UspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="154" mass="16732" checksum="918B831F5E3EDB5B" modified="2017-05-10" version="1">MYERILIPTDGSKVAKAAVDQAVDIAEKYDAEVHALYVADTDAISYSLGTEQVDRIRQGNFEGMTELREEAKAATGYVAEQAAEHGLESVEHHAGGQPHRLISSYAEDHGIDLVVMGSHGRSGVRRALLGSVTERTLRSTHVPVLVVDYQGTDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZP0</accession>
    <name>A0A1U7EZP0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_5360A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324379.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZP0"/>
    <dbReference type="STRING" id="348780.NP_5360A"/>
    <dbReference type="EnsemblBacteria" id="CAI50771">
        <property type="protein sequence ID" value="CAI50771"/>
        <property type="gene ID" value="NP_5360A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702350"/>
    <dbReference type="KEGG" id="nph:NP_5360A"/>
    <dbReference type="eggNOG" id="arCOG06427">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1067957_0_0_2"/>
    <dbReference type="OrthoDB" id="242590at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="273" mass="30977" checksum="9D60BE3B030753C5" modified="2017-05-10" version="1">MLPSPVDATTAKRKLVDTYDNPSYEDPWDAVEDYEHVQRYTAAHPQQGSQAVSTATDLPRGRIRSWVDSDGMPDCYRGLQTALTNGWILDSWSDETARPMAMLAAWTLASGSINEHWTPTWVTDGDDEADALRHHADRANVVIEQAREAEDDRPAEWRPAESASVLGRVLYTWLGHRGDKNHAVPFPAFLESAPDQIRLDFARVHTQQRGIIRDDRPDRFVQIMAERSEGFRRALKELLQSVVGNPDDIRGDSWPLRIYGDAIDTLRQYPELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZP6</accession>
    <name>A0A1U7EZP6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Flavin reductase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">NP_5370A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the flavoredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324384.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZP6"/>
    <dbReference type="STRING" id="348780.NP_5370A"/>
    <dbReference type="EnsemblBacteria" id="CAI50776">
        <property type="protein sequence ID" value="CAI50776"/>
        <property type="gene ID" value="NP_5370A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702355"/>
    <dbReference type="KEGG" id="nph:NP_5370A"/>
    <dbReference type="eggNOG" id="arCOG02017">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1363682_0_0_2"/>
    <dbReference type="OrthoDB" id="8522at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002563">
        <property type="entry name" value="Flavin_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33798:SF5">
        <property type="entry name" value="FLAVIN REDUCTASE LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33798">
        <property type="entry name" value="FLAVOPROTEIN OXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01613">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavin reductase like" evidence="5">
        <location>
            <begin position="31"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI50776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="207" mass="22642" checksum="FA0C629A89876873" modified="2017-05-10" version="1">MSHQTMGLTDDRDLVTLQPDQPFFETLYTVSPLVVIGTREADGSENLAPKHMAFPLGWGDHFGFVCKPAHGTYENVERTGEFTVSYPRPDEVLEASLAAAPRDSEGDKPTLEDIETATGESVNAPAVADAYAVLECELDRIVAGFGDAGLVAGKAVAKHVHTDAHRTHDEEPEVLLEQAPALAYLYPDRFAEIRETQAFPFPEGFER</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILS6</accession>
    <name>Q3ILS6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3332A</name>
        <name evidence="2" type="ordered locus">NP_7056A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>PL23</name>
    </geneLocation>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
            <strain evidence="2">Gabara</strain>
            <plasmid evidence="2">PL23</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CR936259">
        <property type="protein sequence ID" value="CAI50944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323378.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILS6"/>
    <dbReference type="STRING" id="348780.NP_3332A"/>
    <dbReference type="EnsemblBacteria" id="CAI49757">
        <property type="protein sequence ID" value="CAI49757"/>
        <property type="gene ID" value="NP_3332A"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI50944">
        <property type="protein sequence ID" value="CAI50944"/>
        <property type="gene ID" value="NP_7056A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703488"/>
    <dbReference type="KEGG" id="nph:NP_3332A"/>
    <dbReference type="KEGG" id="nph:NP_7056A"/>
    <dbReference type="HOGENOM" id="CLU_2257448_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="103" mass="11891" checksum="94251A877FE172B6" modified="2005-11-08" version="1">MRGHAGTKDVPEFPQIEDELGYNPARWLHTRSESGLDTGPGQTPELATAMSIVKGIDDPEELAYWFKVEKRCRDRDHVIRKLEQRRRAIKYGHDGFVKERDGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILS9</accession>
    <name>Q3ILS9_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3326A</name>
        <name evidence="4" type="ordered locus">NP_7050A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4 5">
        <name>PL23</name>
    </geneLocation>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
            <strain evidence="4">Gabara</strain>
            <plasmid evidence="4">PL23</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CR936259">
        <property type="protein sequence ID" value="CAI50941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323375.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILS9"/>
    <dbReference type="EnsemblBacteria" id="CAI49754">
        <property type="protein sequence ID" value="CAI49754"/>
        <property type="gene ID" value="NP_3326A"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI50941">
        <property type="protein sequence ID" value="CAI50941"/>
        <property type="gene ID" value="NP_7050A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703485"/>
    <dbReference type="KEGG" id="nph:NP_3326A"/>
    <dbReference type="KEGG" id="nph:NP_7050A"/>
    <dbReference type="HOGENOM" id="CLU_2893355_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="49"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="62" mass="7247" checksum="A60FE54C2CCD6BD6" modified="2005-11-08" version="1">MKYEPEKKEIPGMPGTKLRKTKRSYSEQERQEILDRLTEDAAQDAEDDDKDDDGFVSQEELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILX7</accession>
    <name>Q3ILX7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">IS1341-type transposase ISNph18</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">NP_6224A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7 8">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324490.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILX7"/>
    <dbReference type="EnsemblBacteria" id="CAI50892">
        <property type="protein sequence ID" value="CAI50892"/>
        <property type="gene ID" value="NP_6224A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694643"/>
    <dbReference type="KEGG" id="nph:NP_6224A"/>
    <dbReference type="HOGENOM" id="CLU_032903_16_6_2"/>
    <dbReference type="OrthoDB" id="284225at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="5">
        <location>
            <begin position="183"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="6">
        <location>
            <begin position="312"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI50892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="420" mass="47283" checksum="AE9339C447863925" modified="2005-11-08" version="1">MSNQGVTRTLKASISNHSQVCDDLDSHGFAASKLWNVGRWTISRVWDAIGHIPDADELCSYLKSHERYADLHSQSSQRVLQELGEAFVSWYEQDDPDANPPGYRKHGDDHPRSTVTWKNQGFKLDTQYNRVRLSKGTNMKESRYAADYILCEYTLQTDEQTLNAVESVQTVRAVWTDEQWELHFVCKMRVETPETPGEKTAGVDLGICNTAAISVGDETLLYPGNALKEDAHYFRQQEYETAGENGPSDHAEWARQKKSRRQAHFLHAVSKDIVEQCAGRNVGTIAVGHPKNIREDEDWGRHGNKRLHDWAFETLLSHLEYKAQERGIDVERVDEAGLKTSKTCCECGTEADSNRVERGLYVCANCELVANSDLNAAENMRATVTPNPTQDRSNGCLAQPSVRLFDKSTGRVAPQEQVVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILY7</accession>
    <name>Q3ILY7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Formamidase</fullName>
            <ecNumber evidence="2">3.5.1.49</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">fmdA</name>
        <name evidence="2" type="ordered locus">NP_6204A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.1.49" evidence="2"/>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324480.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILY7"/>
    <dbReference type="EnsemblBacteria" id="CAI50882">
        <property type="protein sequence ID" value="CAI50882"/>
        <property type="gene ID" value="NP_6204A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694684"/>
    <dbReference type="KEGG" id="nph:NP_6204A"/>
    <dbReference type="HOGENOM" id="CLU_032013_0_1_2"/>
    <dbReference type="OrthoDB" id="42832at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004328">
        <property type="term" value="F:formamidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.580">
        <property type="entry name" value="Acetamidase/Formamidase-like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004304">
        <property type="entry name" value="FmdA_AmdA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054833">
        <property type="entry name" value="FormamaseFmdA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045496">
        <property type="entry name" value="FormamaseFmdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31891">
        <property type="entry name" value="FORMAMIDASE C869.04-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31891:SF1">
        <property type="entry name" value="FORMAMIDASE C869.04-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03069">
        <property type="entry name" value="FmdA_AmdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141130">
        <property type="entry name" value="Acetamidase/Formamidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="424" mass="46228" checksum="F2463AB0914500D9" modified="2005-11-08" version="1">MPEVKFEVDVDKAPDEQPGANPFNRWHPDIPAVIEAEPGESMRLETLDWTGGQITDNDDPNEVRDVDLSQVHYLAGPVYVNGAEPGDMLKVEFLDMGPLNGRSEFGFTGTFSQKNGGGFLTDHFPHAAKSIWDIEGHTVSSRHIPGVEYEGKIHPGLAGCAPSKELLETWNEREQKLIEKHKEDPESIPNHPTGEPEPGVANPPTSEGALMGEMDPEQAEEAAQEAARTVPPREHGGNHDIKDLSIGSTVYFPVYVEGGKFGIGDFHASQGDGEISFCGAIEMAAYVDVKFDLVKGGMEKFGVEHPIFEPGHRGPHFEDYITFCGYSVTEDGEQRYIDSHTAYRRACLQAIDYLKQFGYTGQQALHILGTVPVEGRQSGVVDIPNACSTLALPKGAFDFDISPDGIEQNSADRGDLVVTDDPLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM57</accession>
    <name>Q3IM57_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">HTH domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_6048A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM57"/>
    <dbReference type="EnsemblBacteria" id="CAI50804">
        <property type="protein sequence ID" value="CAI50804"/>
        <property type="gene ID" value="NP_6048A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_6048A"/>
    <dbReference type="HOGENOM" id="CLU_105300_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05403">
        <property type="entry name" value="NT_KNTase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013196">
        <property type="entry name" value="HTH_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052548">
        <property type="entry name" value="Type_VII_TA_antitoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33933">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33933:SF1">
        <property type="entry name" value="PROTEIN ADENYLYLTRANSFERASE MNTA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08279">
        <property type="entry name" value="HTH_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helix-turn-helix type 11" evidence="2">
        <location>
            <begin position="31"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Polymerase nucleotidyl transferase" evidence="1">
        <location>
            <begin position="131"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="229" mass="25483" checksum="D999B003A9A57278" modified="2005-11-08" version="1">MSEMAKQDIRICIDAYPGPDTDVFRISAADNILRLLADAHETEFTIPELVDATGVTRSTVWRAVDLLNSIGAIQIRETPQRNYISINPDRLQKDDPILGIPQSEFHAPIRAFVDRVQATTADADDINDLLGIVVFGSVARGEADRQSDIDCFVVVDGDRTTARRQITDVVADLQSERFDGDRFVFEPYVESVESAGRAGSKLHEIFTEGITVYRSDRLDALRKEVVADE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM76</accession>
    <name>Q3IM76_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_6006A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324391.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM76"/>
    <dbReference type="EnsemblBacteria" id="CAI50783">
        <property type="protein sequence ID" value="CAI50783"/>
        <property type="gene ID" value="NP_6006A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694577"/>
    <dbReference type="KEGG" id="nph:NP_6006A"/>
    <dbReference type="HOGENOM" id="CLU_958523_0_0_2"/>
    <dbReference type="OrthoDB" id="377648at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="290" mass="31914" checksum="C7A040100F447996" modified="2005-11-08" version="1">MSVERVREELLAGGLHKESLERLLDHYESMSNRFSEGEFAEAGTHIGNFCENMVNILLDQMGEEVQKRPNVDYFVNKCLSNGIGNDEPDSVRLQIPRVIRAAYDIRNNRDSVHVNLKVPVNRADTQAGIAMCSWMLAEILRVYGNGTDNMGEIAGLIDEISEPADDKNPLEDLAQSPDEFDRQALADQLSGVVQITEGTVHPITGSDYEELSTTGQVIVLLLGHRAAVDISYVENAGASVPDLSEETDVSDERVRQIIRDFGFIQAGNASGEYMIPGHRVGEALKQLSLG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q3INF0</accession>
    <name>KHSE_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Homoserine kinase</fullName>
            <shortName evidence="1">HK</shortName>
            <shortName evidence="1">HSK</shortName>
            <ecNumber evidence="1">2.7.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thrB</name>
        <name type="ordered locus">NP_4524A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-homoserine + ATP = O-phospho-L-homoserine + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13985"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57590"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.39"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GHMP kinase family. Homoserine kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.39" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323968.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3INF0"/>
    <dbReference type="SMR" id="Q3INF0"/>
    <dbReference type="STRING" id="348780.NP_4524A"/>
    <dbReference type="EnsemblBacteria" id="CAI50353">
        <property type="protein sequence ID" value="CAI50353"/>
        <property type="gene ID" value="NP_4524A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703252"/>
    <dbReference type="KEGG" id="nph:NP_4524A"/>
    <dbReference type="eggNOG" id="arCOG01027">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041243_1_1_2"/>
    <dbReference type="OrthoDB" id="28273at2157"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00064"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004413">
        <property type="term" value="F:homoserine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00384">
        <property type="entry name" value="Homoser_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000870">
        <property type="entry name" value="Homoserine_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002288">
        <property type="entry name" value="PRK01212.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00191">
        <property type="entry name" value="thrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861:SF1">
        <property type="entry name" value="HOMOSERINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861">
        <property type="entry name" value="HOMOSERINE/4-DIPHOSPHOCYTIDYL-2-C-METHYL-D-ERYTHRITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000676">
        <property type="entry name" value="Homoser_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00958">
        <property type="entry name" value="HOMSERKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0791">Threonine biosynthesis</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000049153" description="Homoserine kinase">
        <location>
            <begin position="1"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="80"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00384"/>
        </source>
    </evidence>
    <sequence length="291" mass="29485" checksum="8F11B745057EE066" modified="2005-11-08" version="1">MITVRAPATSANLGSGFDVFGAALERPADILRIEKANRTTIRVTGVGSKYIPEDPEKNTVGAVAEALDAPAHIEIDKGVRPASGLGSSAASAAAAAVGLNELYDRGLSREELVPIAAEGEAVVSGAAHADNVAPSIMGGFTVAREDGVTQVDASIPLVACLPEIVVSTRDARGVVPEAAPMEAVVDVVGNAATLAVGMARDDPALVGRGMEDSIVTPERAELINGYETVRSAAENAGATGVTISGAGPTVIAACHRGDRTAIASAMLDAFSEAGVEARAYKTEIGRGAELF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-04-17" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q3INK4</accession>
    <name>Y4416_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-binding protein NP_4416A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">NP_4416A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PDCD5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323914.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3INK4"/>
    <dbReference type="SMR" id="Q3INK4"/>
    <dbReference type="STRING" id="348780.NP_4416A"/>
    <dbReference type="EnsemblBacteria" id="CAI50299">
        <property type="protein sequence ID" value="CAI50299"/>
        <property type="gene ID" value="NP_4416A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702055"/>
    <dbReference type="KEGG" id="nph:NP_4416A"/>
    <dbReference type="eggNOG" id="arCOG04179">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122978_3_0_2"/>
    <dbReference type="OrthoDB" id="7912at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.140">
        <property type="entry name" value="PDCD5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00026">
        <property type="entry name" value="dsDNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022889">
        <property type="entry name" value="DNA_bind_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002836">
        <property type="entry name" value="PDCD5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036883">
        <property type="entry name" value="PDCD5-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003268">
        <property type="entry name" value="PRK04239.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10840">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10840:SF0">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01984">
        <property type="entry name" value="dsDNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015730">
        <property type="entry name" value="TFAR19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46950">
        <property type="entry name" value="Double-stranded DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000284567" description="DNA-binding protein NP_4416A">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="12"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="29"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="115" mass="13129" checksum="C986F8FCEECC5FA6" modified="2005-11-08" version="1">MSGEPTDEDLEELRKKKMEQLKEQGGEGQSEAAEAQRQQAEAQKKAILRKTLTDGARKRLNTVQMSKPQFGEKVEQQIVALAQSGRIQGKIDEEKMKELLQEMKPDSQSFDIKRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-28" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q3INU3</accession>
    <name>BIOB_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Biotin synthase</fullName>
            <ecNumber evidence="1">2.8.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">bioB</name>
        <name type="ordered locus">NP_4236A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(4R,5S)-dethiobiotin + (sulfur carrier)-SH + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = (sulfur carrier)-H + biotin + 2 5'-deoxyadenosine + 2 L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:22060"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="ChEBI" id="CHEBI:149473"/>
            <dbReference type="EC" id="2.8.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="1">Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the radical SAM superfamily. Biotin synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.6" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323825.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3INU3"/>
    <dbReference type="SMR" id="Q3INU3"/>
    <dbReference type="STRING" id="348780.NP_4236A"/>
    <dbReference type="EnsemblBacteria" id="CAI50209">
        <property type="protein sequence ID" value="CAI50209"/>
        <property type="gene ID" value="NP_4236A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702551"/>
    <dbReference type="KEGG" id="nph:NP_4236A"/>
    <dbReference type="eggNOG" id="arCOG00658">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033172_2_1_2"/>
    <dbReference type="OrthoDB" id="9264at2157"/>
    <dbReference type="UniPathway" id="UPA00078">
        <property type="reaction ID" value="UER00162"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004076">
        <property type="term" value="F:biotin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01694">
        <property type="entry name" value="BioB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010722">
        <property type="entry name" value="BATS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002684">
        <property type="entry name" value="Biotin_synth/BioAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024177">
        <property type="entry name" value="Biotin_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00433">
        <property type="entry name" value="bioB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22976">
        <property type="entry name" value="BIOTIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22976:SF2">
        <property type="entry name" value="BIOTIN SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06968">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001619">
        <property type="entry name" value="Biotin_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01060">
        <property type="entry name" value="BATS_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01278">
        <property type="entry name" value="biotin_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00876">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0001">2Fe-2S</keyword>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0093">Biotin biosynthesis</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000381491" description="Biotin synthase">
        <location>
            <begin position="1"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="50"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="328"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="352"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="370" mass="40111" checksum="88B1B20CE3A318FE" modified="2005-11-08" version="1">MVYETGNETVDDAVQRALDGERLDRTDGLALLAQPVDELAAGADLLRRHFSDGTVDACSIVNAKAGNCAEDCGFCAQSAHFDTGIDTYGFLDPEDILDAAKRAEADGAQRFGIVVAEKGVSKEKRPDEWDDVIRAIRLVRDETDVEVDASLGVLTEEEAEILAAEGLNHYNHNIETSRRYFSEIVNTHSFDDRLKTLHRAKAAGMDLCAGVILGMGETPADRVDAAIELQEVGVSSLPVNILNPVEGTPIGDREAATISRTELIKTIAVYRFLHPEARVRLTGGREVNLDPDEQHLPFEAGADGLLTGDYLTTDGQTPADDIEIIERAGLEPNREANTFDPESVKARHRSPAAETASNANRTNATTETDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IQS3</accession>
    <name>Q3IQS3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aspartate-rich protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2860A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323146.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IQS3"/>
    <dbReference type="STRING" id="348780.NP_2860A"/>
    <dbReference type="EnsemblBacteria" id="CAI49521">
        <property type="protein sequence ID" value="CAI49521"/>
        <property type="gene ID" value="NP_2860A"/>
    </dbReference>
    <dbReference type="GeneID" id="59558370"/>
    <dbReference type="KEGG" id="nph:NP_2860A"/>
    <dbReference type="eggNOG" id="ENOG502N5TW">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_833182_0_0_2"/>
    <dbReference type="OrthoDB" id="351383at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="333" mass="37827" checksum="44453FBD00BB294B" modified="2005-11-08" version="1">MQPSRRTFVKSGIGVVGLGALAGCAEEDPDPGAEDEETTPDEEEPDEETPEPETASVTLLVEGVGGHDHDDDDDHDHDDDHDHDDHHDDDHDHDDHHDDDHDHDDHHDDDHDHDDDDHDHDDDDHDDHDDHHDDHHDDDHHDDDDHHDDHSEGLSAGEIDHACGHIEFDDFEPLEAGTSSDTAPTISSTHQPYDVTVEGEGGYIRFEVEDDDHHDDHDHHDDDHHDDHDHHDDHDHHDDDHHDDHDHHDDHDHDDDHHDDDHHDDHDHDDDDHHDDHDHGGDKLAFFTQGGSVTVHTGHTEHEEAGIEGCDGLDRYVIVEPDHGEAVIELVAE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-10-05" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IRF2</accession>
    <name>COFC_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">2-phospho-L-lactate guanylyltransferase</fullName>
            <shortName evidence="1">LP guanylyltransferase</shortName>
            <ecNumber evidence="1">2.7.7.68</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cofC</name>
        <name type="ordered locus">NP_2400A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Guanylyltransferase that catalyzes the activation of (2S)-2-phospholactate (2-PL) as (2S)-lactyl-2-diphospho-5'-guanosine, via the condensation of 2-PL with GTP. It is involved in the biosynthesis of coenzyme F420, a hydride carrier cofactor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2S)-2-phospholactate + GTP + H(+) = (2S)-lactyl-2-diphospho-5'-guanosine + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:63424"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:59435"/>
            <dbReference type="ChEBI" id="CHEBI:59906"/>
            <dbReference type="EC" id="2.7.7.68"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme F420 biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CofC family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.68" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322917.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IRF2"/>
    <dbReference type="SMR" id="Q3IRF2"/>
    <dbReference type="STRING" id="348780.NP_2400A"/>
    <dbReference type="EnsemblBacteria" id="CAI49291">
        <property type="protein sequence ID" value="CAI49291"/>
        <property type="gene ID" value="NP_2400A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701435"/>
    <dbReference type="KEGG" id="nph:NP_2400A"/>
    <dbReference type="eggNOG" id="arCOG04472">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076569_2_0_2"/>
    <dbReference type="OrthoDB" id="11179at2157"/>
    <dbReference type="UniPathway" id="UPA00071"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043814">
        <property type="term" value="F:phospholactate guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052645">
        <property type="term" value="P:F420-0 metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02114">
        <property type="entry name" value="CofC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002835">
        <property type="entry name" value="CofC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03552">
        <property type="entry name" value="F420_cofC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40392">
        <property type="entry name" value="2-PHOSPHO-L-LACTATE GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40392:SF1">
        <property type="entry name" value="2-PHOSPHO-L-LACTATE GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01983">
        <property type="entry name" value="CofC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000398762" description="2-phospho-L-lactate guanylyltransferase">
        <location>
            <begin position="1"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02114"/>
        </source>
    </evidence>
    <sequence length="201" mass="21084" checksum="0483E7ED041E56AF" modified="2005-11-08" version="1">MDVLVPFAAREPKTRLAGTLDADERAAFARAMLLDVCDAVEAAGGEPTVLATEPLAIEQPVVVDKRPLTPAIDAHLSPPAAVVMADVALVTPAALRRLFATTGDVVIAPGLGGGTNALVVRADGFTVDYHGTSVRDHRRAAEGAGIDARSVDSFRLAVDIDEPTDLAEVLLHGDGRSADWLKNAGFRLAETDGRTTVERRG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IRQ2</accession>
    <name>ILVC_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ketol-acid reductoisomerase (NADP(+))</fullName>
            <shortName evidence="1">KARI</shortName>
            <ecNumber evidence="1">1.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Acetohydroxy-acid isomeroreductase</fullName>
            <shortName evidence="1">AHIR</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Alpha-keto-beta-hydroxylacyl reductoisomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Ketol-acid reductoisomerase type 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Ketol-acid reductoisomerase type I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ilvC</name>
        <name type="ordered locus">NP_2198A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (2S)-2-acetolactate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22068"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="1.1.1.86"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2-ethyl-2-hydroxy-3-oxobutanoate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13493"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49256"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.86"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ketol-acid reductoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.86" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322818.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IRQ2"/>
    <dbReference type="SMR" id="Q3IRQ2"/>
    <dbReference type="STRING" id="348780.NP_2198A"/>
    <dbReference type="EnsemblBacteria" id="CAI49190">
        <property type="protein sequence ID" value="CAI49190"/>
        <property type="gene ID" value="NP_2198A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702867"/>
    <dbReference type="KEGG" id="nph:NP_2198A"/>
    <dbReference type="eggNOG" id="arCOG04465">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033821_0_1_2"/>
    <dbReference type="OrthoDB" id="6064at2157"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00056"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00060"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004455">
        <property type="term" value="F:ketol-acid reductoisomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000023">
        <property type="entry name" value="Ketol-acid reductoisomerase (NADP(+))"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IlvC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
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        <property type="entry name" value="KARI"/>
    </dbReference>
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        <property type="entry name" value="KARI_C"/>
    </dbReference>
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        <property type="entry name" value="KARI_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014359">
        <property type="entry name" value="KARI_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="PRK13403.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21371">
        <property type="entry name" value="KETOL-ACID REDUCTOISOMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21371:SF1">
        <property type="entry name" value="KETOL-ACID REDUCTOISOMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01450">
        <property type="entry name" value="KARI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07991">
        <property type="entry name" value="KARI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000116">
        <property type="entry name" value="IlvC_gammaproteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51851">
        <property type="entry name" value="KARI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51850">
        <property type="entry name" value="KARI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000226218" description="Ketol-acid reductoisomerase (NADP(+))">
        <location>
            <begin position="1"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="KARI N-terminal Rossmann" evidence="2">
        <location>
            <begin position="4"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="KARI C-terminal knotted" evidence="3">
        <location>
            <begin position="184"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="109"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="27"/>
            <end position="30"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="85"/>
            <end position="88"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01198"/>
        </source>
    </evidence>
    <sequence length="331" mass="35966" checksum="9AC1D028799918D9" modified="2005-11-08" version="1">MTDATIYYDDDAESTVLDDKTVAVLGYGSQGHAHAQNLDDSGVDVVVGLREDSSSRSAAEADGLDVATPRGAAEQADLVSVLVPDTVQPAVYEQIEDVLQPGDTLQFAHGFNIHYGQIEPSEDVNVTMVAPKSPGHLVRRNYENDEGTPGLLAVYQDPSGEAHDLGLAYAKAIGCTRAGVVETTFREETETDLFGEQAVLCGGVTSLVKTGYETLVDAGYSPEMAYFECLNELKLIVDLMYEGGNSEMWDSVSDTAEYGGLTRGDRIVDDHAREKMEEVLEEVQNGTFAREWISENQAGRPSYKQLRAAEKNHDIEAVGEDLRALFAWGDD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ISI7</accession>
    <name>RS27_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein eS27</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">30S ribosomal protein S27e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps27e</name>
        <name type="ordered locus">NP_1616A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS27 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322533.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ISI7"/>
    <dbReference type="SMR" id="Q3ISI7"/>
    <dbReference type="STRING" id="348780.NP_1616A"/>
    <dbReference type="EnsemblBacteria" id="CAI48899">
        <property type="protein sequence ID" value="CAI48899"/>
        <property type="gene ID" value="NP_1616A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703178"/>
    <dbReference type="KEGG" id="nph:NP_1616A"/>
    <dbReference type="eggNOG" id="arCOG04108">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_199465_0_0_2"/>
    <dbReference type="OrthoDB" id="5718at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.100">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00371">
        <property type="entry name" value="Ribosomal_eS27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000592">
        <property type="entry name" value="Ribosomal_eS27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023407">
        <property type="entry name" value="Ribosomal_eS27_Zn-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001629">
        <property type="entry name" value="PRK00415.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01667">
        <property type="entry name" value="Ribosomal_S27e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_1000007135" description="Small ribosomal subunit protein eS27">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="1">
        <location>
            <begin position="10"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="57" mass="5939" checksum="406A983E9298504D" modified="2005-11-08" version="1">MAGDFIRVHCDDCENEQVLFGKAANTVNCAVCGSTLATPTGGEADLHGDVVDVVESR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IT33</accession>
    <name>GATB_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B</fullName>
            <shortName evidence="1">Asp/Glu-ADT subunit B</shortName>
            <ecNumber evidence="1">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gatB</name>
        <name type="ordered locus">NP_1220A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-aspartyl-tRNA(Asn) + L-glutamine + ATP + H2O = L-asparaginyl-tRNA(Asn) + L-glutamate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14513"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="Rhea" id="RHEA-COMP:9677"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GatB/GatE family. GatB subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322337.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IT33"/>
    <dbReference type="SMR" id="Q3IT33"/>
    <dbReference type="STRING" id="348780.NP_1220A"/>
    <dbReference type="EnsemblBacteria" id="CAI48701">
        <property type="protein sequence ID" value="CAI48701"/>
        <property type="gene ID" value="NP_1220A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702360"/>
    <dbReference type="KEGG" id="nph:NP_1220A"/>
    <dbReference type="eggNOG" id="arCOG01718">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019240_0_0_2"/>
    <dbReference type="OrthoDB" id="52755at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050566">
        <property type="term" value="F:asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070681">
        <property type="term" value="P:glutaminyl-tRNAGln biosynthesis via transamidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.410:FF:000001">
        <property type="entry name" value="Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.380">
        <property type="entry name" value="GatB domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00121">
        <property type="entry name" value="GatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017959">
        <property type="entry name" value="Asn/Gln-tRNA_amidoTrfase_suB/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006075">
        <property type="entry name" value="Asn/Gln-tRNA_Trfase_suB/E_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018027">
        <property type="entry name" value="Asn/Gln_amidotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003789">
        <property type="entry name" value="Asn/Gln_tRNA_amidoTrase-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004413">
        <property type="entry name" value="GatB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042114">
        <property type="entry name" value="GatB_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023168">
        <property type="entry name" value="GatB_Yqey_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017958">
        <property type="entry name" value="Gln-tRNA_amidoTrfase_suB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00133">
        <property type="entry name" value="gatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004012">
        <property type="entry name" value="PRK05477.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004014">
        <property type="entry name" value="PRK05477.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659">
        <property type="entry name" value="GLUTAMYL-TRNA GLN AMIDOTRANSFERASE SUBUNIT B MITOCHONDRIAL AND PROKARYOTIC PET112-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659:SF0">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02934">
        <property type="entry name" value="GatB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02637">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00845">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89095">
        <property type="entry name" value="GatB/YqeY motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01234">
        <property type="entry name" value="GATB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000241304" description="Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B">
        <location>
            <begin position="1"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00121"/>
        </source>
    </evidence>
    <sequence length="496" mass="54734" checksum="4563380F6E58AE3B" modified="2005-11-08" version="1">MTAQTAEEHSLTAVIGLEVHVQLETDTKIFCGCSTDADDNEEPNTRTCPVCLGLPGALPVLNEGAVEAAVKVGKALSSDIPEETRFHRKNYYYPDLPKNFQITQYDAPICDGGELEFAVDDERRTVAIERAHLEEDPGSLSHAGGSIDTAEHTLVDYNRAGTPLLEIVTAPDFRSAAEVRAFLAKLTEVLEYLGVFDVTRDGSLRVDANLSIVDSDAVGENGSIDEATLEAANRTEVKNISSHKGAEKALAYEETRQKNAVQRGREVEQETRHWDESRGITVSMRSKEEEKDYRYFREADIPPLRVSDWKEKIDIPELPDARRERFREEYGLDAETASKLTSTKQVADFYERVADTFAPELAATWVADNLLGELNYRDMEITDIEGRLDEFAHLIELVDEDEITVKNAEEVVLRRMLDDGQPPGDIVEAEDLGKTDDDAVAAAVTAAIEENPDAVEDYHAGEDGALNFLVGQVMQETGGSADPGTVNELLRAELDG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ITB6</accession>
    <name>COFE_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Coenzyme F420:L-glutamate ligase</fullName>
            <ecNumber evidence="1">6.3.2.31</ecNumber>
            <ecNumber evidence="1">6.3.2.34</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Coenzyme F420-0:L-glutamate ligase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Coenzyme F420-1:gamma-L-glutamate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cofE</name>
        <name type="ordered locus">NP_1054A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the GTP-dependent successive addition of two or more gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form coenzyme F420-0-glutamyl-glutamate (F420-2) or polyglutamated F420 derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>oxidized coenzyme F420-0 + GTP + L-glutamate = oxidized coenzyme F420-1 + GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:30555"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:59907"/>
            <dbReference type="ChEBI" id="CHEBI:59920"/>
            <dbReference type="EC" id="6.3.2.31"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>oxidized coenzyme F420-1 + GTP + L-glutamate = oxidized coenzyme F420-2 + GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:30523"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57922"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:59920"/>
            <dbReference type="EC" id="6.3.2.34"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 2 divalent metal cations per subunit. The ions could be magnesium and/or manganese.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="1">Monovalent cation. The ion could be potassium.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme F420 biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CofE family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.31" evidence="1"/>
    <dbReference type="EC" id="6.3.2.34" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322253.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ITB6"/>
    <dbReference type="SMR" id="Q3ITB6"/>
    <dbReference type="STRING" id="348780.NP_1054A"/>
    <dbReference type="EnsemblBacteria" id="CAI48618">
        <property type="protein sequence ID" value="CAI48618"/>
        <property type="gene ID" value="NP_1054A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701040"/>
    <dbReference type="KEGG" id="nph:NP_1054A"/>
    <dbReference type="eggNOG" id="arCOG02714">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051152_1_1_2"/>
    <dbReference type="OrthoDB" id="11383at2157"/>
    <dbReference type="UniPathway" id="UPA00071"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052618">
        <property type="term" value="F:coenzyme F420-0:L-glutamate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052619">
        <property type="term" value="F:coenzyme F420-1:gamma-L-glutamate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052645">
        <property type="term" value="P:F420-0 metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.100">
        <property type="entry name" value="CofE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1660.10">
        <property type="entry name" value="CofE-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01258">
        <property type="entry name" value="F420_ligase_CofE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008225">
        <property type="entry name" value="F420-0_g-glutamyl_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002847">
        <property type="entry name" value="F420-0_gamma-glut_ligase-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023659">
        <property type="entry name" value="F420_ligase_CofE_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01916">
        <property type="entry name" value="F420_cofE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009809">
        <property type="entry name" value="PRK13293.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47917">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47917:SF1">
        <property type="entry name" value="COENZYME F420:L-GLUTAMATE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01996">
        <property type="entry name" value="F420_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144010">
        <property type="entry name" value="CofE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0630">Potassium</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000067259" description="Coenzyme F420:L-glutamate ligase">
        <location>
            <begin position="1"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="9"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="38"/>
            <end position="39"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="204"/>
            <end position="211"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01258"/>
        </source>
    </evidence>
    <sequence length="253" mass="27293" checksum="E7BD565323DCCE5B" modified="2005-11-08" version="1">MEALAVDGLPEIHEGDDLAALLEDRVDFADGDVLCVASTIVSKAEGRAFDRESFPPSDRAKAIADRLSTITGEQKDPRFAQAVLEESEELLTESPFLLSVTRFGHITVNAGIDRSNVPGADLLLLPEDPTASAERLSSALGVPVVVTDTSGRPFRYGQRGVAVGWAGLPAARDWRGETDRDGRELGVTVQAVVDELAATANLVAGEGDDGTPAVVVREWSFGDHDGSDLLFRREEDDIVREALRQWTFDGHQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ITM2</accession>
    <name>Q3ITM2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Mrp-type sodium/proton antiporter system subunit F</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">mrpF</name>
        <name evidence="8" type="synonym">tp49b</name>
        <name evidence="8" type="ordered locus">NP_0840A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322147.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ITM2"/>
    <dbReference type="STRING" id="348780.NP_0840A"/>
    <dbReference type="EnsemblBacteria" id="CAI48511">
        <property type="protein sequence ID" value="CAI48511"/>
        <property type="gene ID" value="NP_0840A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703329"/>
    <dbReference type="KEGG" id="nph:NP_0840A"/>
    <dbReference type="eggNOG" id="arCOG03120">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125825_2_0_2"/>
    <dbReference type="OrthoDB" id="84883at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007208">
        <property type="entry name" value="MrpF/PhaF-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009244">
        <property type="entry name" value="PRK12599.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34702">
        <property type="entry name" value="NA(+)/H(+) ANTIPORTER SUBUNIT F1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34702:SF1">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER SUBUNIT F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04066">
        <property type="entry name" value="MrpF_PhaF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI48511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="94" mass="10040" checksum="0148270440CACB6F" modified="2005-11-08" version="1">MTEYASAAMVLGAAVVVVLTVAVLYRVVAGPTTHDRVVAINVIGTSTVIVIAFVAAGLDRPEYLDIAIVYAVLNFVLSLAVGRFAYRDGEVKWR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ITQ4</accession>
    <name>Y776_NATPD</name>
    <protein>
        <recommendedName>
            <fullName>UPF0213 protein NP_0776A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">NP_0776A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPF0213 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322115.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ITQ4"/>
    <dbReference type="SMR" id="Q3ITQ4"/>
    <dbReference type="STRING" id="348780.NP_0776A"/>
    <dbReference type="EnsemblBacteria" id="CAI48479">
        <property type="protein sequence ID" value="CAI48479"/>
        <property type="gene ID" value="NP_0776A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700959"/>
    <dbReference type="KEGG" id="nph:NP_0776A"/>
    <dbReference type="eggNOG" id="arCOG04722">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135650_0_3_2"/>
    <dbReference type="OrthoDB" id="297764at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd10456">
        <property type="entry name" value="GIY-YIG_UPF0213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1440.10">
        <property type="entry name" value="GIY-YIG endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000305">
        <property type="entry name" value="GIY-YIG_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035901">
        <property type="entry name" value="GIY-YIG_endonuc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050190">
        <property type="entry name" value="UPF0213_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34477">
        <property type="entry name" value="UPF0213 PROTEIN YHBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34477:SF1">
        <property type="entry name" value="UPF0213 PROTEIN YHBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01541">
        <property type="entry name" value="GIY-YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82771">
        <property type="entry name" value="GIY-YIG endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50164">
        <property type="entry name" value="GIY_YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000063674" description="UPF0213 protein NP_0776A">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="GIY-YIG" evidence="1">
        <location>
            <begin position="3"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="58"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="58"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="85" mass="9605" checksum="C58DF0064F3E4677" modified="2005-11-08" version="1">MAADHYVYVLSCADETLYTGYTTNVERRVAEHDAGEGAKYTRGRTPVELVHTERFDSKSAAMQREHEIKSLSRAKKERLVADETG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-09-02" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ITS6</accession>
    <name>PURO_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">IMP cyclohydrolase</fullName>
            <ecNumber evidence="1">3.5.4.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">IMP synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Inosinicase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purO</name>
        <name type="ordered locus">NP_0732A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the cyclization of 5-formylamidoimidazole-4-carboxamide ribonucleotide to IMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:18445"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="EC" id="3.5.4.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal IMP cyclohydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.10" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322093.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ITS6"/>
    <dbReference type="SMR" id="Q3ITS6"/>
    <dbReference type="STRING" id="348780.NP_0732A"/>
    <dbReference type="EnsemblBacteria" id="CAI48457">
        <property type="protein sequence ID" value="CAI48457"/>
        <property type="gene ID" value="NP_0732A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703076"/>
    <dbReference type="KEGG" id="nph:NP_0732A"/>
    <dbReference type="eggNOG" id="arCOG04727">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1352116_0_0_2"/>
    <dbReference type="OrthoDB" id="92928at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00135"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003937">
        <property type="term" value="F:IMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.20">
        <property type="entry name" value="Inosine monophosphate cyclohydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00705">
        <property type="entry name" value="IMP_cyclohydrol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010191">
        <property type="entry name" value="IMP_cyclohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020600">
        <property type="entry name" value="IMP_cyclohydrolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036795">
        <property type="entry name" value="IMP_cyclohydrolase-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003167">
        <property type="entry name" value="PRK04151.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01922">
        <property type="entry name" value="purO_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07826">
        <property type="entry name" value="IMP_cyclohyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004866">
        <property type="entry name" value="IMP_cclhdr_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75569">
        <property type="entry name" value="Archaeal IMP cyclohydrolase PurO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000349169" description="IMP cyclohydrolase">
        <location>
            <begin position="1"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00705"/>
        </source>
    </evidence>
    <sequence length="191" mass="20776" checksum="4A64B740BE9897C2" modified="2005-11-08" version="1">MYVGRFIVVAPDRAAYRVSSRSFPNRRIVDRDGTLTVGPTEDAPETDNPYISYNCLRTVGDDYAVVGNGTQVDPIAEKLSLGYPPRDALAESLLALDYEKDDYDTPRIAGVVGDESYIGTVRRDALIVEAVEEPTLVATYEKSEPEPTSLGADDPSELAAELYDRDLEHPVCAAGVVADGDSFEVGYYNGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IUD8</accession>
    <name>EF1A_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Elongation factor 1-alpha</fullName>
            <shortName evidence="2">EF-1-alpha</shortName>
            <ecNumber evidence="2">3.6.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Elongation factor Tu</fullName>
            <shortName evidence="2">EF-Tu</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">tuf</name>
        <name type="ordered locus">NP_0304A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">GTP hydrolase that promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.3" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321881.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IUD8"/>
    <dbReference type="SMR" id="Q3IUD8"/>
    <dbReference type="STRING" id="348780.NP_0304A"/>
    <dbReference type="EnsemblBacteria" id="CAI48243">
        <property type="protein sequence ID" value="CAI48243"/>
        <property type="gene ID" value="NP_0304A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703230"/>
    <dbReference type="KEGG" id="nph:NP_0304A"/>
    <dbReference type="eggNOG" id="arCOG01561">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007265_0_0_2"/>
    <dbReference type="OrthoDB" id="371718at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:GTP binding"/>
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    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
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    <dbReference type="CDD" id="cd03693">
        <property type="entry name" value="EF1_alpha_II"/>
        <property type="match status" value="1"/>
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    <dbReference type="CDD" id="cd03705">
        <property type="entry name" value="EF1_alpha_III"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="2.40.30.10:FF:000003">
        <property type="entry name" value="Elongation factor 1-alpha"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="EF_Tu_A"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="EFTu-like_2"/>
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        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004539">
        <property type="entry name" value="Transl_elong_EF1A_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00483">
        <property type="entry name" value="EF-1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008969">
        <property type="entry name" value="PRK12317.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0251">Elongation factor</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000015710" description="Elongation factor 1-alpha">
        <location>
            <begin position="1"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="tr-type G">
        <location>
            <begin position="5"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="G1" evidence="1">
        <location>
            <begin position="14"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="G2" evidence="1">
        <location>
            <begin position="70"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="G3" evidence="1">
        <location>
            <begin position="91"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="G4" evidence="1">
        <location>
            <begin position="146"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="G5" evidence="1">
        <location>
            <begin position="185"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="14"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="91"/>
            <end position="95"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="146"/>
            <end position="149"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00118"/>
        </source>
    </evidence>
    <sequence length="422" mass="45986" checksum="BF0263B30F90DF15" modified="2005-11-08" version="1">MSEDKPHQNLAVIGHVDHGKSTMVGRLLFETGSVPEHVIEQYREEAEEKGKGGFEFAYVMDNLAEERERGVTIDIAHQEFDTDEYYFTIVDCPGHRDFVKNMITGASQADNAVLVVAADDGVQPQTQEHVFLARTLGIDELIIAVNKMDLVDYDENKYKAVVDEVNQLLEQVRFNTEDAKFIPTSAFEGDNVSEASENTSWYDGPSLLEALNDLPEPQPPTDAPLRLPIQDVYTISGIGTVPVGRVETGILNTGDNVSFQPSDVSGEVKTVEMHHEEVPKAEPGDNVGFNVRGVGKDDIRRGDVCGPADDPPSVAETFQAQVVVMQHPSVITAGYTPVFHAHTAQVACTIESIDKKIDPSSGEVAEENPDFIQSGDAAVVTVRPQKPLSIESSNEIPELGSFAIRDMGQTVAAGKVLSVNER</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-26" modified="2026-01-28" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IUP1</accession>
    <name>SRP54_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Signal recognition particle 54 kDa protein</fullName>
            <shortName evidence="1">SRP54</shortName>
            <ecNumber evidence="1">3.6.5.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">srp54</name>
        <name type="ordered locus">NP_0096A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.4"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. Archaeal SRP consists of a 7S RNA molecule of 300 nucleotides and two protein subunits: SRP54 and SRP19.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">The SRP-RNC complex is targeted to the cytoplasmic membrane.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP-binding SRP family. SRP54 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.4" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321778.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IUP1"/>
    <dbReference type="SMR" id="Q3IUP1"/>
    <dbReference type="STRING" id="348780.NP_0096A"/>
    <dbReference type="EnsemblBacteria" id="CAI48139">
        <property type="protein sequence ID" value="CAI48139"/>
        <property type="gene ID" value="NP_0096A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703217"/>
    <dbReference type="KEGG" id="nph:NP_0096A"/>
    <dbReference type="eggNOG" id="arCOG01228">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009301_6_0_2"/>
    <dbReference type="OrthoDB" id="52849at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048500">
        <property type="term" value="C:signal recognition particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17875">
        <property type="entry name" value="SRP54_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="entry name" value="Signal recognition particle SRP54, nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.30">
        <property type="entry name" value="Signal recognition particle, SRP54 subunit, M-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00306">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036891">
        <property type="entry name" value="Signal_recog_part_SRP54_M_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004125">
        <property type="entry name" value="Signal_recog_particle_SRP54_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022941">
        <property type="entry name" value="SRP54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 54K PROTEIN SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564:SF5">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE SUBUNIT SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02978">
        <property type="entry name" value="SRP_SPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="Domain of the SRP/SRP receptor G-proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47446">
        <property type="entry name" value="Signal peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0733">Signal recognition particle</keyword>
    <feature type="chain" id="PRO_0000322245" description="Signal recognition particle 54 kDa protein">
        <location>
            <begin position="1"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="444"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="446"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="104"/>
            <end position="111"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="184"/>
            <end position="188"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="242"/>
            <end position="245"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="466" mass="50640" checksum="67B8C2211BB90320" modified="2005-11-08" version="1">MVLDDLGSSLRGTLDKLQGKTTLSEDDVEEIVKEIQRSLLSADVDVDLVMDLSDSIRERALDEEPPAGTTARDHVLKIVYEEMVELVGESTALPLEEQTIVLAGLQGSGKTTTAAKMAWWFSKKGLRPAVIQTDTFRPGAYDQAKEMCERAEVDFYGNPDSDDPVEIAERGLEETADADVHIVDTAGRHALEEALIDELEAIESAVDPDRNLLVLDAAIGQGAKDQAERFHDAVGIDGVAITKLDGTAKGGGALAAVDQTDSSIAFLGTGEEVKDIERFEPSGFISRLLGMGDLKQLSERVERAMEETQAEEDWDPEDLMKGEFTLKDMRNQMNAMNKMGPLDQVMDMIPGLGGGIKDQLPDDAMDMTQERLRDFEVIMDSMSEAELENPRAIGQSQIERIARGSGTDEDTVRELLEQHKMMSRMMKQFQGMGDGDMQRMMKQMQQGGGGGGGGGGGLGGMGPFGD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-26" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IUQ3</accession>
    <name>RIFK_NATPD</name>
    <protein>
        <recommendedName>
            <fullName>Riboflavin kinase</fullName>
            <shortName>RFK</shortName>
            <ecNumber>2.7.1.161</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>CTP-dependent riboflavin kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>CTP:riboflavin 5'-phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Flavokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">ribK</name>
        <name type="ordered locus">NP_0072A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>riboflavin + CTP = CDP + FMN + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25021"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:57986"/>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="2.7.1.161"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal riboflavin kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.161"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321766.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IUQ3"/>
    <dbReference type="SMR" id="Q3IUQ3"/>
    <dbReference type="STRING" id="348780.NP_0072A"/>
    <dbReference type="EnsemblBacteria" id="CAI48127">
        <property type="protein sequence ID" value="CAI48127"/>
        <property type="gene ID" value="NP_0072A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700753"/>
    <dbReference type="KEGG" id="nph:NP_0072A"/>
    <dbReference type="eggNOG" id="arCOG01904">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088476_0_0_2"/>
    <dbReference type="OrthoDB" id="30955at2157"/>
    <dbReference type="UniPathway" id="UPA00276">
        <property type="reaction ID" value="UER00929"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008531">
        <property type="term" value="F:riboflavin kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009398">
        <property type="term" value="P:FMN biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.30">
        <property type="entry name" value="Riboflavin kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01285">
        <property type="entry name" value="Riboflavin_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039063">
        <property type="entry name" value="RibK_CTP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023470">
        <property type="entry name" value="Riboflavin_kinase_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023602">
        <property type="entry name" value="Riboflavin_kinase_CTP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023465">
        <property type="entry name" value="Riboflavin_kinase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40706">
        <property type="entry name" value="RIBOFLAVIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40706:SF1">
        <property type="entry name" value="RIBOFLAVIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01982">
        <property type="entry name" value="CTP-dep_RFKase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12840">
        <property type="entry name" value="HTH_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82114">
        <property type="entry name" value="Riboflavin kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0288">FMN</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000322096" description="Riboflavin kinase">
        <location>
            <begin position="1"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="H-T-H motif-like">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Riboflavin kinase">
        <location>
            <begin position="98"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="107"/>
            <end position="112"/>
        </location>
        <ligand>
            <name>CDP</name>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="212"/>
            <end position="215"/>
        </location>
        <ligand>
            <name>CDP</name>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="232" mass="25603" checksum="7B9F4A021E05D190" modified="2005-11-08" version="1">MSQTTERVGADELSTLKLVALDGALDGRVTVTCADLADRLDASNQTASRRLQRLEAAALVERDMTGEGQRIAVTEAGERALQREYADYRRLFEGDADVTLSGVVTSGMGEGRHYISLSGYMRQFRERLGYEPFPGTLNVDLDDGSVRTRARMDALDPVKIDGWEDDERTYGPAFCWPAVLETADGDRYGTAHVIAPERTHHGDDQLEIIAPEKLRETLSLDDGDTLSIHVSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ET91</accession>
    <name>A0A1U7ET91_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UCP015877 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0016A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321738.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ET91"/>
    <dbReference type="STRING" id="348780.NP_0016A"/>
    <dbReference type="EnsemblBacteria" id="CAI48099">
        <property type="protein sequence ID" value="CAI48099"/>
        <property type="gene ID" value="NP_0016A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700739"/>
    <dbReference type="KEGG" id="nph:NP_0016A"/>
    <dbReference type="eggNOG" id="arCOG02680">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110112_0_0_2"/>
    <dbReference type="OrthoDB" id="23364at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012041">
        <property type="entry name" value="Znf_CPxCG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42195">
        <property type="entry name" value="UCP015877 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42195:SF1">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19769">
        <property type="entry name" value="CPxCG_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015877">
        <property type="entry name" value="UCP015877"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="216" mass="23813" checksum="739D1E9CDD9D40CC" modified="2017-05-10" version="1">MSQQRVAVRCPSCSPAEETAHEVLNDGGQATVRCGACGHVHKTAIETESTVDRRVVVSQDDESTEARVDIPPGESLETGDEFLVETDEAILTARITSLELTSGDRVEEATAEDVRTLWTRAVGNVAVNLTLHPKEGVPEESRSVKIQVPGDEAFVVGDTHEYGGEEFTVERIMVREDATDYDREGYDFQGDTVLAKDVKRVYARDEDARTRAWSGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ET98</accession>
    <name>A0A1U7ET98_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transport protein (Probable substrate cationic amino acids)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cat2</name>
        <name evidence="8" type="synonym">tp01a</name>
        <name evidence="8" type="ordered locus">NP_0042A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321751.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ET98"/>
    <dbReference type="STRING" id="348780.NP_0042A"/>
    <dbReference type="EnsemblBacteria" id="CAI48112">
        <property type="protein sequence ID" value="CAI48112"/>
        <property type="gene ID" value="NP_0042A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703278"/>
    <dbReference type="KEGG" id="nph:NP_0042A"/>
    <dbReference type="eggNOG" id="arCOG00009">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_15_8_2"/>
    <dbReference type="OrthoDB" id="56838at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050367">
        <property type="entry name" value="APC_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770">
        <property type="entry name" value="AMINO ACID TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770:SF7">
        <property type="entry name" value="MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
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        </location>
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        <location>
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        <location>
            <begin position="491"/>
            <end position="623"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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        <source>
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        </source>
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        <source>
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        <source>
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        </source>
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        <source>
            <dbReference type="EMBL" id="CAI48112.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
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    <sequence length="764" mass="80006" checksum="5FCA6B43650EFA0F" modified="2017-05-10" version="1"> </sequence>
</entry>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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</entry>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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            </authorList>
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        </citation>
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        </source>
    </reference>
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    </dbReference>
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        </location>
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        <source>
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            <dbReference type="EMBL" id="CAI48131.1"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="95" mass="9948" checksum="B3829ED651B0555C" modified="2017-05-10" version="1">MASRVEFNATVSTMFGLFLFVVAVGNVVAGSTPFAIMFGALGFVFVSLSSYIRSNPDAFEDGDEEVPDRWIRIVRGGGGLVLLAAAAFVVALALG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
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            </authorList>
            <dbReference type="PubMed" id="16169924"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="EnsemblBacteria" id="CAI48240">
        <property type="protein sequence ID" value="CAI48240"/>
        <property type="gene ID" value="NP_0298A"/>
    </dbReference>
    <dbReference type="GeneID" id="41345075"/>
    <dbReference type="KEGG" id="nph:NP_0298A"/>
    <dbReference type="HOGENOM" id="CLU_2285126_0_0_2"/>
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    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="101" mass="11118" checksum="D61B20B232E29592" modified="2017-05-10" version="1">MSYEKPVECALCGEGLFEKGPQGNPQRIGETELVVTSDENVAKMEIPPKKRGEVTRAFALKIGLCPDCIDDYDLQGSGELADTYDDIVLHRAKILPKKEPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETL4</accession>
    <name>A0A1U7ETL4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0324A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48253.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939399.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETL4"/>
    <dbReference type="STRING" id="348780.NP_0324A"/>
    <dbReference type="EnsemblBacteria" id="CAI48253">
        <property type="protein sequence ID" value="CAI48253"/>
        <property type="gene ID" value="NP_0324A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700820"/>
    <dbReference type="KEGG" id="nph:NP_0324A"/>
    <dbReference type="eggNOG" id="arCOG09169">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_527492_0_0_2"/>
    <dbReference type="OrthoDB" id="239360at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="350"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="414"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="466"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="486"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="507"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48253.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="516" mass="53566" checksum="DE8EEF1BFC9574BC" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETN5</accession>
    <name>A0A1U7ETN5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0358A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321908.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETN5"/>
    <dbReference type="STRING" id="348780.NP_0358A"/>
    <dbReference type="EnsemblBacteria" id="CAI48270">
        <property type="protein sequence ID" value="CAI48270"/>
        <property type="gene ID" value="NP_0358A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700830"/>
    <dbReference type="KEGG" id="nph:NP_0358A"/>
    <dbReference type="eggNOG" id="arCOG03927">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114808_0_0_2"/>
    <dbReference type="OrthoDB" id="300230at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045396">
        <property type="entry name" value="DUF6517"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20127">
        <property type="entry name" value="DUF6517"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="188" mass="20101" checksum="0527BBC890D99A44" modified="2017-05-10" version="1">MSVPVPESALDGGWTHAKNRSRTVFEGFGVSVRAETVIYEDADLRERIAAAGGPDRVWRFFFSSRLAVSPSPPLLETVGRPHAVRESKQDFAAELRARGIEGVSAGDTRSVRVADAEAALTPFYGTVDADGATVDVVGALALWYEDGFLVAGGAYPSDGVERWVDVAPDAFETELLSLIRATAETAQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETP7</accession>
    <name>A0A1U7ETP7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Creatininase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cre1</name>
        <name evidence="5" type="ordered locus">NP_0414A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321936.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETP7"/>
    <dbReference type="STRING" id="348780.NP_0414A"/>
    <dbReference type="EnsemblBacteria" id="CAI48298">
        <property type="protein sequence ID" value="CAI48298"/>
        <property type="gene ID" value="NP_0414A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700849"/>
    <dbReference type="KEGG" id="nph:NP_0414A"/>
    <dbReference type="eggNOG" id="arCOG04536">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055029_2_1_2"/>
    <dbReference type="OrthoDB" id="46121at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10310">
        <property type="entry name" value="Creatininase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024087">
        <property type="entry name" value="Creatininase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003785">
        <property type="entry name" value="Creatininase/forma_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35005:SF1">
        <property type="entry name" value="2-AMINO-5-FORMYLAMINO-6-RIBOSYLAMINOPYRIMIDIN-4(3H)-ONE 5'-MONOPHOSPHATE DEFORMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35005">
        <property type="entry name" value="3-DEHYDRO-SCYLLO-INOSOSE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02633">
        <property type="entry name" value="Creatininase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102215">
        <property type="entry name" value="Creatininase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI48298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="242" mass="25747" checksum="D07D6D9C6B72FB04" modified="2017-05-10" version="1">MHLSEATWTDIRDATTDLAVLPVGSTEQHGPHAPLGVDWLTAEAIAEAGVDRYADTHGEKPLVGPTIPVGVAEEHRDFDGTLWVSPDTFRSYVGETVASLASHGFERVVIVNGHGGNVDALNEVCARLTRDGDAYAVQYTWFDAVDFASLGHGGPAETAVMRHLHPELVREDRTDTAAEGAADSWGEWVSGTNLAVDSSSFTESGAVGDPREGDADLGEELFEKAAAALVELLDAVERRSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETP8</accession>
    <name>A0A1U7ETP8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Abi/CAAX domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0418A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321938.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETP8"/>
    <dbReference type="STRING" id="348780.NP_0418A"/>
    <dbReference type="EnsemblBacteria" id="CAI48300">
        <property type="protein sequence ID" value="CAI48300"/>
        <property type="gene ID" value="NP_0418A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700850"/>
    <dbReference type="KEGG" id="nph:NP_0418A"/>
    <dbReference type="eggNOG" id="arCOG02769">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051806_4_0_2"/>
    <dbReference type="OrthoDB" id="331240at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080120">
        <property type="term" value="P:CAAX-box protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1/LyrA-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39430">
        <property type="entry name" value="MEMBRANE-ASSOCIATED PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39430:SF1">
        <property type="entry name" value="PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="275"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="CAAX prenyl protease 2/Lysostaphin resistance protein A-like" evidence="2">
        <location>
            <begin position="138"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="319" mass="32553" checksum="C63DF1409F22DA23" modified="2017-05-10" version="1">MASVWFVRGRLRTPWRFLATGLLVVFATVVASLPVAIVDVPLDPRAATGEALALVLGYQAVVGVAIAAAVVIAARHVDRRTMADLGLGRQRWLRELAIGGVLGAALMTGAYAAGVVAGVYRPTFAPAGPDGYSLAVWFGLCAVFVIVVGVYEELLFRGYVLTNLAEGLTAVLSPRRAVIAAILLSGAGFGVVHGGNPNMTPLGVATIAVAGLLLGLGYGYTGRIALPVGFHITWNAAHFVYGLPVSGLELGIRVVETERVGSALLHGGSVGPEGGVFGFIAAAVGCLAVVAYGRAVSGGLDETVAVPAIRSGADDNSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETP9</accession>
    <name>A0A1U7ETP9_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dihydroneopterin aldolase</fullName>
            <shortName evidence="1">DHNA</shortName>
            <ecNumber evidence="1">4.1.2.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">7,8-dihydroneopterin aldolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1 3" type="primary">mptD</name>
        <name evidence="3" type="ordered locus">NP_0408A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of 7,8-dihydroneopterin (H2Neo) to 6-hydroxymethyl-7,8-dihydropterin (6-HMD).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7,8-dihydroneopterin = 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde</text>
            <dbReference type="Rhea" id="RHEA:10540"/>
            <dbReference type="ChEBI" id="CHEBI:17001"/>
            <dbReference type="ChEBI" id="CHEBI:17071"/>
            <dbReference type="ChEBI" id="CHEBI:44841"/>
            <dbReference type="EC" id="4.1.2.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal dihydroneopterin aldolase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.25" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321933.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETP9"/>
    <dbReference type="STRING" id="348780.NP_0408A"/>
    <dbReference type="EnsemblBacteria" id="CAI48295">
        <property type="protein sequence ID" value="CAI48295"/>
        <property type="gene ID" value="NP_0408A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700846"/>
    <dbReference type="KEGG" id="nph:NP_0408A"/>
    <dbReference type="eggNOG" id="arCOG04705">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149105_1_0_2"/>
    <dbReference type="OrthoDB" id="132689at2157"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00154"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004150">
        <property type="term" value="F:dihydroneopterin aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1300.20">
        <property type="entry name" value="7,8-dihydroneopterin aldolase (MptD)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02130">
        <property type="entry name" value="DHNA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027508">
        <property type="entry name" value="DHN_aldolase_MptD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036839">
        <property type="entry name" value="MptD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007181">
        <property type="entry name" value="MtpD_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04038">
        <property type="entry name" value="DHNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143560">
        <property type="entry name" value="MK0786-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="1 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dihydroneopterin aldolase MtpD C-terminal" evidence="2">
        <location>
            <begin position="5"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="115" mass="12527" checksum="88B0F56B4C9696C5" modified="2017-05-10" version="1">MKPTTPQEACFEAGIKFGTLYHQFAGTPVAPESSDSLAAAMEDAIENQPFCTAVTVDILDEKLRKTLAEQSADYTELTGEYMEVELIIEYDGCTVAASMAMDGDYPRMQVDDVDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETQ9</accession>
    <name>A0A1U7ETQ9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Homolog to glycine cleavage system protein T</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">gcvT2</name>
        <name evidence="5" type="ordered locus">NP_0436A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321945.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETQ9"/>
    <dbReference type="STRING" id="348780.NP_0436A"/>
    <dbReference type="EnsemblBacteria" id="CAI48309">
        <property type="protein sequence ID" value="CAI48309"/>
        <property type="gene ID" value="NP_0436A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702747"/>
    <dbReference type="KEGG" id="nph:NP_0436A"/>
    <dbReference type="eggNOG" id="arCOG00757">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007884_6_3_2"/>
    <dbReference type="OrthoDB" id="299960at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="entry name" value="Probable tRNA modification gtpase trme, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028896">
        <property type="entry name" value="GcvT/YgfZ/DmdA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029043">
        <property type="entry name" value="GcvT/YgfZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013977">
        <property type="entry name" value="GcvT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006222">
        <property type="entry name" value="GCVT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017703">
        <property type="entry name" value="YgfZ/GCV_T_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03317">
        <property type="entry name" value="ygfZ_signature"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43757">
        <property type="entry name" value="AMINOMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43757:SF2">
        <property type="entry name" value="AMINOMETHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01571">
        <property type="entry name" value="GCV_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08669">
        <property type="entry name" value="GCV_T_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006487">
        <property type="entry name" value="GcvT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101790">
        <property type="entry name" value="Aminomethyltransferase beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103025">
        <property type="entry name" value="Folate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="GCVT N-terminal" evidence="3">
        <location>
            <begin position="7"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Aminomethyltransferase C-terminal" evidence="4">
        <location>
            <begin position="275"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR006487-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI48309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="360" mass="38762" checksum="F6314B274367A049" modified="2017-05-10" version="1">MTVVAELQESYGATFTERGGRQVVDHYGRPERVHRAVRNVVGTVEMGYGIIDVGGDDRLEFVDNAVTNRVPETDGAGSYALLLDPQGGIETELYIYNAGERLLCFVPPGRAEPVAEEWADKTFIQDVDINVATDDYGVFGVHGPKATEKVASVLTGPTTPEEPLEFVRGRVGDWGTTVIRTDGLTGEEGYEIVCAADDAANVFDALVNHGLNAAPFGYRTLEYLWLEAGTPLFETELEGTVPNVLGLRNALDFEKGCYVGQEVVSKIENQGRPSRELVGIKLDAEPTAGAAVFDGDSHVGEVTRGDYSPALDGAIALALVEYGLESDDLTVRIDSDDVAAERVQLPFVDGSARSARLPSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETS6</accession>
    <name>A0A1U7ETS6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0496A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETS6"/>
    <dbReference type="STRING" id="348780.NP_0496A"/>
    <dbReference type="EnsemblBacteria" id="CAI48339">
        <property type="protein sequence ID" value="CAI48339"/>
        <property type="gene ID" value="NP_0496A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_0496A"/>
    <dbReference type="HOGENOM" id="CLU_3362599_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="35" mass="3785" checksum="B617042CE0F6304C" modified="2017-05-10" version="1">MTASGHERGDRKRLREWASLTGDTLPAEEAGGEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETT1</accession>
    <name>A0A1U7ETT1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Signal peptidase I</fullName>
            <ecNumber evidence="7">3.4.21.89</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">sec11</name>
        <name evidence="7" type="ordered locus">NP_0484A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.21.89" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321969.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETT1"/>
    <dbReference type="STRING" id="348780.NP_0484A"/>
    <dbReference type="EnsemblBacteria" id="CAI48333">
        <property type="protein sequence ID" value="CAI48333"/>
        <property type="gene ID" value="NP_0484A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703199"/>
    <dbReference type="KEGG" id="nph:NP_0484A"/>
    <dbReference type="eggNOG" id="arCOG01739">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054902_0_1_2"/>
    <dbReference type="OrthoDB" id="4822at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009003">
        <property type="term" value="F:signal peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001733">
        <property type="entry name" value="Peptidase_S26B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX CATALYTIC SUBUNIT SEC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806:SF6">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX CATALYTIC SUBUNIT SEC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="LexA/Signal peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="13"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="266"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="276" mass="29647" checksum="137006F691963EE9" modified="2017-05-10" version="1">MSDRTDGGREGDPSETEPEPEGDPFEYPAAEDRDDGDGETGDSPEGLVEWVRWFRTTDDGAVVYLRDIVTSVAAVLLVGAVLFGISGIWPPMVAVESGSMEPNMERGDLIFIVDNERFTPDEAISNDGSTTGVVPADVAAEHDRTKFSDHGDVIIFLPNGEHPIQVIHRAMLWVEDGENWYDRADPDAIGGAENCDELEACPAPNGGFITKGDDNGDYDQVNQLSDPVKPEWITGTAEVRIPYLGHIRLTFAGQPAPPVTEPVGATNATSAGPQAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU24</accession>
    <name>A0A1U7EU24_NATPD</name>
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        <submittedName>
            <fullName evidence="4">Receiver box response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_0682A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU24"/>
    <dbReference type="STRING" id="348780.NP_0682A"/>
    <dbReference type="EnsemblBacteria" id="CAI48432">
        <property type="protein sequence ID" value="CAI48432"/>
        <property type="gene ID" value="NP_0682A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700928"/>
    <dbReference type="KEGG" id="nph:NP_0682A"/>
    <dbReference type="eggNOG" id="arCOG06315">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_8_2"/>
    <dbReference type="OrthoDB" id="8127at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050595">
        <property type="entry name" value="Bact_response_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010916">
        <property type="entry name" value="TonB_box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44591:SF3">
        <property type="entry name" value="RESPONSE REGULATORY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44591">
        <property type="entry name" value="STRESS RESPONSE REGULATOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00430">
        <property type="entry name" value="TONB_DEPENDENT_REC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="5"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="134" mass="14444" checksum="A2C03784C57C2AD4" modified="2017-05-10" version="1">MSGPAVLLVEDSGFMASHVSGTLEAAHGMDVRVAETAAEARQRLRDNDIDCLVVNTQLPDENGVEFVRSIQQSADLPSMPSLLFTGEFLEDVAAEAFDAGVVDVVSKEHHAGDSMVVFANRIKVAIDAFEQRLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU37</accession>
    <name>A0A1U7EU37_NATPD</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0742A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322098.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU37"/>
    <dbReference type="STRING" id="348780.NP_0742A"/>
    <dbReference type="EnsemblBacteria" id="CAI48462">
        <property type="protein sequence ID" value="CAI48462"/>
        <property type="gene ID" value="NP_0742A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700950"/>
    <dbReference type="KEGG" id="nph:NP_0742A"/>
    <dbReference type="eggNOG" id="arCOG14780">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1105235_0_0_2"/>
    <dbReference type="OrthoDB" id="242855at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="239" mass="24356" checksum="F29D13A854BDA52E" modified="2017-05-10" version="1">MSVRLYLRSPLALAWTLLAVVLVGAVVLGGWLLAAALTPAAAAGSELLGGLLSLGIGVVAVALAAVLWVPAGAAIAYIVGHRARGRSVSAGATNAALRRHRVDIYRWCKTRVAVVDRLAERLLDEADVSRTEVAAGCGPYVVPALLLDAPRLPAAVERANRVVPNPGRQRLLYRGTAATFAAAAVVAIPGVVVRGGVDEFVGGAVLAVAVVGAVVTAALDAAWRASTYAQAATEDGFVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU47</accession>
    <name>A0A1U7EU47_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0768A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU47"/>
    <dbReference type="STRING" id="348780.NP_0768A"/>
    <dbReference type="EnsemblBacteria" id="CAI48475">
        <property type="protein sequence ID" value="CAI48475"/>
        <property type="gene ID" value="NP_0768A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_0768A"/>
    <dbReference type="eggNOG" id="arCOG02977">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092285_1_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055951">
        <property type="entry name" value="DUF7529"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24373">
        <property type="entry name" value="DUF7529"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="161" mass="17710" checksum="C790B16ABB11D3BF" modified="2017-05-10" version="1">MLLPLGDPVSDMQQGPPPAVTDRWETLREDATATAAEYDADGWTVHRVETGEVTPLSGTPFGLDVLVPGDDFERASELVEAASFDTSHVYRAEERGVRFLIIVVEASDDEVAVVVPAYLSASDTEALAERAREEGVMYTHLRPLSDEARVTFSHEDPELFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU60</accession>
    <name>A0A1U7EU60_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Small CPxCG-related zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0778A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322116.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU60"/>
    <dbReference type="STRING" id="348780.NP_0778A"/>
    <dbReference type="EnsemblBacteria" id="CAI48480">
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        <property type="gene ID" value="NP_0778A"/>
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    <dbReference type="KEGG" id="nph:NP_0778A"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_210071_1_0_2"/>
    <dbReference type="OrthoDB" id="189700at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055985">
        <property type="entry name" value="DUF7563"/>
    </dbReference>
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        <property type="entry name" value="DUF7563"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="48" mass="5271" checksum="0F06E267D9520D57" modified="2017-05-10" version="1">MPRCDHCDSHVSDRFARVFADDHGRVRACPNCSANAGIAEVSLDRIAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU66</accession>
    <name>A0A1U7EU66_NATPD</name>
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        <submittedName>
            <fullName evidence="2">DUF457 family protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">NP_0820A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU66"/>
    <dbReference type="STRING" id="348780.NP_0820A"/>
    <dbReference type="EnsemblBacteria" id="CAI48501">
        <property type="protein sequence ID" value="CAI48501"/>
        <property type="gene ID" value="NP_0820A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700971"/>
    <dbReference type="KEGG" id="nph:NP_0820A"/>
    <dbReference type="eggNOG" id="arCOG04590">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1567080_0_0_2"/>
    <dbReference type="OrthoDB" id="199847at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007404">
        <property type="entry name" value="YdjM-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04307">
        <property type="entry name" value="YdjM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="162" mass="17253" checksum="050AF84CD23E9242" modified="2017-05-10" version="1">MNKEGHVLNAVVLGVGLGFVLEPAGSMETLRTVVAVTVPVVLGALFPDVDTAFGKHRKTLHNLPILGLFVAFPFVFGNLSYVWIGVLTHYVLDYLGSRRGIALFYPLSSTEYGSPFGVTTSSRFANAVTLIVTGVELAVAVAIVHVLPAYVDIAELTAQLPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUB3</accession>
    <name>A0A1U7EUB3_NATPD</name>
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        <submittedName>
            <fullName evidence="2">GNAT family acetyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0904A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322179.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUB3"/>
    <dbReference type="STRING" id="348780.NP_0904A"/>
    <dbReference type="EnsemblBacteria" id="CAI48543">
        <property type="protein sequence ID" value="CAI48543"/>
        <property type="gene ID" value="NP_0904A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700995"/>
    <dbReference type="KEGG" id="nph:NP_0904A"/>
    <dbReference type="eggNOG" id="arCOG04721">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132452_0_0_2"/>
    <dbReference type="OrthoDB" id="194677at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13508">
        <property type="entry name" value="Acetyltransf_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="118" mass="12328" checksum="C7918299FCCBDBB1" modified="2017-05-10" version="1">MTTVRSATPGEYPAVRSILNAAMLTVPSGLLARGSVLVACDDDRILGGLVLVGDSIEAVAVRPGRRGQGLGTALIERAAARRPRLRAGFDPSVRPFYDSLGFDITCDDGRCEGVLDAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUE5</accession>
    <name>A0A1U7EUE5_NATPD</name>
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        <submittedName>
            <fullName evidence="12">DNA repair helicase Rad3</fullName>
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        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">rad3a</name>
        <name evidence="12" type="ordered locus">NP_0960A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.4.12" evidence="12"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48571.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322207.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUE5"/>
    <dbReference type="STRING" id="348780.NP_0960A"/>
    <dbReference type="EnsemblBacteria" id="CAI48571">
        <property type="protein sequence ID" value="CAI48571"/>
        <property type="gene ID" value="NP_0960A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703092"/>
    <dbReference type="KEGG" id="nph:NP_0960A"/>
    <dbReference type="eggNOG" id="arCOG00770">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_313456_0_0_2"/>
    <dbReference type="OrthoDB" id="27512at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ATP-dep_Helicase_C"/>
    </dbReference>
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        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014013">
        <property type="entry name" value="Helic_SF1/SF2_ATP-bd_DinG/Rad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010614">
        <property type="entry name" value="RAD3-like_helicase_DEAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="DNA REPAIR DEAD HELICASE RAD3/XP-D SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472:SF34">
        <property type="entry name" value="REGULATOR OF TELOMERE ELONGATION HELICASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06733">
        <property type="entry name" value="DEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51193">
        <property type="entry name" value="HELICASE_ATP_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="17"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="396"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="155"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI48571.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="794" mass="86881" checksum="8F0DAFC267F65C17" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUF2</accession>
    <name>A0A1U7EUF2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">A-type ATP synthase subunit F</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">atpF</name>
        <name evidence="7" type="ordered locus">NP_1028A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Has multiple subunits with at least A(3), B(3), C, D, E, F, H, I and proteolipid K(x).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the V-ATPase F subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322240.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUF2"/>
    <dbReference type="STRING" id="348780.NP_1028A"/>
    <dbReference type="EnsemblBacteria" id="CAI48605">
        <property type="protein sequence ID" value="CAI48605"/>
        <property type="gene ID" value="NP_1028A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702543"/>
    <dbReference type="KEGG" id="nph:NP_1028A"/>
    <dbReference type="eggNOG" id="arCOG04102">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135754_2_2_2"/>
    <dbReference type="OrthoDB" id="24971at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10580">
        <property type="entry name" value="ATPase, V1 complex, subunit F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00312">
        <property type="entry name" value="ATP_synth_F_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008218">
        <property type="entry name" value="ATPase_V1-cplx_f_g_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022944">
        <property type="entry name" value="ATPase_V1-cplx_fsu_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036906">
        <property type="entry name" value="ATPase_V1_fsu_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002577">
        <property type="entry name" value="PRK02228.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01990">
        <property type="entry name" value="ATP-synt_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159468">
        <property type="entry name" value="AtpF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="106" mass="11491" checksum="B71ABA50E19D7B82" modified="2017-05-10" version="1">MSQEIAVVGSPDFTTGFRLAGVRRFENVSDADKDEQLDDAVTSVLEDDDVGIVVMHDDDVEVLSRTVRQRVETSVEPVVVTLGGGTGSGNLREQIKRAIGIDLMDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUF4</accession>
    <name>A0A1U7EUF4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">A-type ATP synthase subunit H</fullName>
            <ecNumber evidence="2">3.6.3.14</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">atpH</name>
        <name evidence="2" type="ordered locus">NP_1018A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.14" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322235.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUF4"/>
    <dbReference type="STRING" id="348780.NP_1018A"/>
    <dbReference type="EnsemblBacteria" id="CAI48600">
        <property type="protein sequence ID" value="CAI48600"/>
        <property type="gene ID" value="NP_1018A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702544"/>
    <dbReference type="KEGG" id="nph:NP_1018A"/>
    <dbReference type="eggNOG" id="arCOG03363">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149095_1_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028987">
        <property type="entry name" value="ATP_synth_B-like_membr_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014275">
        <property type="entry name" value="ATPase_A1A0-cplx_hsu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02926">
        <property type="entry name" value="AhaH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81573">
        <property type="entry name" value="F1F0 ATP synthase subunit B, membrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="112" mass="13019" checksum="6B5A435AD319625B" modified="2017-05-10" version="1">MARPEVLEQIKEAEGEADDIVAEAEQEREERIAEAREEADSIREEAQEEAQQLKQERLEEAREEIEAEREDILAEGESEREALESRAEENREEVIEYTVELFKEAVDVQSQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUG4</accession>
    <name>A0A1U7EUG4_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1044A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322248.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUG4"/>
    <dbReference type="STRING" id="348780.NP_1044A"/>
    <dbReference type="EnsemblBacteria" id="CAI48613">
        <property type="protein sequence ID" value="CAI48613"/>
        <property type="gene ID" value="NP_1044A"/>
    </dbReference>
    <dbReference type="GeneID" id="54763310"/>
    <dbReference type="KEGG" id="nph:NP_1044A"/>
    <dbReference type="eggNOG" id="arCOG06947">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3113156_0_0_2"/>
    <dbReference type="OrthoDB" id="189688at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="1">
        <location>
            <begin position="3"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="1">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="50" mass="5478" checksum="0D01FB1D68E32245" modified="2017-05-10" version="1">MSYACSACDSEFESAAGVTQHVALHHDTCAVCNESFGETDELREHVHQSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUP5</accession>
    <name>A0A1U7EUP5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">FAD-dependent oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1230A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322342.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUP5"/>
    <dbReference type="STRING" id="348780.NP_1230A"/>
    <dbReference type="EnsemblBacteria" id="CAI48706">
        <property type="protein sequence ID" value="CAI48706"/>
        <property type="gene ID" value="NP_1230A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701089"/>
    <dbReference type="KEGG" id="nph:NP_1230A"/>
    <dbReference type="eggNOG" id="arCOG02200">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1297469_0_0_2"/>
    <dbReference type="OrthoDB" id="35401at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00322">
        <property type="entry name" value="FNR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050415">
        <property type="entry name" value="MRET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47354">
        <property type="entry name" value="NADH OXIDOREDUCTASE HCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47354:SF5">
        <property type="entry name" value="PROTEIN RFBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="215" mass="21913" checksum="1ECD6A43736DB9FC" modified="2017-05-10" version="1">MDPIETTVAAVREVGPDAVAIDIETPSGFEAAPGQFVKLSAELDGDTTGRFYTVSSPDTEAVFEITVGYDAEEAGAFSEYLQAIEAGDTVTITGPFGNDYYEGEPRAVIIAGGPGVGPAVAIAERALADGHDAAVVYVDDEPLHEDRLASLSAGGANVLVLNGAEQLGDAVADAVTNGDGEQVFVYGFSSFLEDATAAIEAAGGDADAAKTENFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUS0</accession>
    <name>A0A1U7EUS0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Replication protein A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rpa3</name>
        <name evidence="2" type="ordered locus">NP_1282A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322367.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUS0"/>
    <dbReference type="STRING" id="348780.NP_1282A"/>
    <dbReference type="EnsemblBacteria" id="CAI48732">
        <property type="protein sequence ID" value="CAI48732"/>
        <property type="gene ID" value="NP_1282A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701099"/>
    <dbReference type="KEGG" id="nph:NP_1282A"/>
    <dbReference type="eggNOG" id="arCOG01510">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031172_0_0_2"/>
    <dbReference type="OrthoDB" id="335252at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010212">
        <property type="term" value="P:response to ionizing radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04491">
        <property type="entry name" value="SoSSB_OBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000301">
        <property type="entry name" value="Replication protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051231">
        <property type="entry name" value="SOSS-B"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005553">
        <property type="entry name" value="PRK07217.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13356">
        <property type="entry name" value="OB FOLD NUCLEIC ACID BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13356:SF8">
        <property type="entry name" value="REPLICATION PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="309" mass="34347" checksum="057149B834498CB2" modified="2017-05-10" version="1">MSDLREHATEIHEQFSDAIDESVDETESRLETLVSEYQVPVEEARRSVVSTYIDESDIDRDELSGGSSQSAEVTDIDAPEEWLDLTVKVVDLWEPGADSIAQVGLLGDETGTVKFTKWQKSDLPALEEDEVYRLENVVTDEYQGRFSVKLNSTTNIERLDTDIEVGDDTTETEGALVDIQSGSGLIKRCPKPDCTRVLQNGRCSEHGEVEGEFDLRIKGVLDDGTEVTEVIFDKEATEELTGITLEEAQQMAMDALDTEVVVEEMREVVLGQYYRVTGPTLGRYVLANDTERLDGPVDAESVLIKARSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUV2</accession>
    <name>A0A1U7EUV2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Thioredoxin-disulfide reductase</fullName>
            <ecNumber evidence="4">1.8.1.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">trxB2</name>
        <name evidence="4" type="ordered locus">NP_1388A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.8.1.9" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUV2"/>
    <dbReference type="STRING" id="348780.NP_1388A"/>
    <dbReference type="EnsemblBacteria" id="CAI48785">
        <property type="protein sequence ID" value="CAI48785"/>
        <property type="gene ID" value="NP_1388A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_1388A"/>
    <dbReference type="eggNOG" id="arCOG01296">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031864_5_0_2"/>
    <dbReference type="OrthoDB" id="27340at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050097">
        <property type="entry name" value="Ferredoxin-NADP_redctase_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48105">
        <property type="entry name" value="THIOREDOXIN REDUCTASE 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="3">
        <location>
            <begin position="5"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="342" mass="36731" checksum="63DA78AFA4E77E34" modified="2017-05-10" version="1">MTDHYEVTVVGGGPAGLTTALYTTRLGHDTAVINRGGGRAAMMLDTHNVIGITEDTSGNELLSRATEQIQEYGADYHRGTVTDIQSTDEGFAVTAGDTEVTTEHVVLATGFSDKRPDPPLPRTGKGLHYCLHCDAYMFVDEPVYVMGTSESAAHVAMIMLNFTPDVDLLTRGEDPEWSDETDTQLRNHPVEVVHEDITGLEKDDDGWLSAFEFEDGTVREYRGGFPMYGSEYNTDLAESLGVELNDDGTIGVDDHGRTNVDGVYAVGDVTPGHNQIPVAMGEGARCGIGNHYDLRAFPRSLDDIESEGPVTEADVPAVSEKLKEAATTHEGHPGAGEAAGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUV4</accession>
    <name>A0A1U7EUV4_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Hydroxymethylbilane synthase</fullName>
            <ecNumber evidence="5">2.5.1.61</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">hemC</name>
        <name evidence="9" type="ordered locus">NP_1326A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>dipyrromethane</name>
            <dbReference type="ChEBI" id="CHEBI:60342"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HMBS family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.61" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322389.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUV4"/>
    <dbReference type="STRING" id="348780.NP_1326A"/>
    <dbReference type="EnsemblBacteria" id="CAI48754">
        <property type="protein sequence ID" value="CAI48754"/>
        <property type="gene ID" value="NP_1326A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702807"/>
    <dbReference type="KEGG" id="nph:NP_1326A"/>
    <dbReference type="eggNOG" id="arCOG04299">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019704_1_0_2"/>
    <dbReference type="OrthoDB" id="8042at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004418">
        <property type="term" value="F:hydroxymethylbilane synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.40">
        <property type="entry name" value="Porphobilinogen deaminase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000860">
        <property type="entry name" value="HemC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022419">
        <property type="entry name" value="Porphobilin_deaminase_cofac_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022417">
        <property type="entry name" value="Porphobilin_deaminase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022418">
        <property type="entry name" value="Porphobilinogen_deaminase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036803">
        <property type="entry name" value="Porphobilinogen_deaminase_C_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00212">
        <property type="entry name" value="hemC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11557">
        <property type="entry name" value="PORPHOBILINOGEN DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11557:SF0">
        <property type="entry name" value="PORPHOBILINOGEN DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01379">
        <property type="entry name" value="Porphobil_deam"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03900">
        <property type="entry name" value="Porphobil_deamC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001438">
        <property type="entry name" value="4pyrrol_synth_OHMeBilane_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00151">
        <property type="entry name" value="PORPHBDMNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54782">
        <property type="entry name" value="Porphobilinogen deaminase (hydroxymethylbilane synthase), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00533">
        <property type="entry name" value="PORPHOBILINOGEN_DEAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Porphobilinogen deaminase N-terminal" evidence="7">
        <location>
            <begin position="5"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Porphobilinogen deaminase N-terminal" evidence="7">
        <location>
            <begin position="213"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="Porphobilinogen deaminase C-terminal" evidence="8">
        <location>
            <begin position="278"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="99"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="157"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="171"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="NCBIfam" id="TIGR00212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI48754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="361" mass="39282" checksum="9970363AFE5DECD3" modified="2017-05-10" version="1">MTETIRLATRGSDLALRQAQRVADALDSRRRTVELVEVETTGDAVRDELIHRLGKTGAFVRSLDEKVIDGDVDAAVHSMKDMPTEFPDELVVAGVPERASPGDVLVTPDGDTLDSLPDGATVGTSSLRRKAQLLAARPDLDVQPLRGNVDTRVQKLLAPSLQAEHERRLEAEDDEGEAEDEDGDDADRYDRPPEEWFDDLAEIERQALGREIETRFDGIVLAEAGLQRTGLLAQVETERLPETFVPAAGQGALAVTACDGDVGQTLQNELDHPRTRVETTVERTILGTLGGGCVAPVGIHAVVRGEYVQASVRVLSQDGTEEIEATRDLPIERHPEAAREFADELADRGAADLIQRARRDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUW4</accession>
    <name>A0A1U7EUW4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Homolog to S-adenosylmethionine-dependent methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1346A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322399.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUW4"/>
    <dbReference type="STRING" id="348780.NP_1346A"/>
    <dbReference type="EnsemblBacteria" id="CAI48764">
        <property type="protein sequence ID" value="CAI48764"/>
        <property type="gene ID" value="NP_1346A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701122"/>
    <dbReference type="KEGG" id="nph:NP_1346A"/>
    <dbReference type="eggNOG" id="arCOG04526">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_884561_0_0_2"/>
    <dbReference type="OrthoDB" id="338984at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="265" mass="29188" checksum="79955C64D355DF93" modified="2017-05-10" version="1">MSDHVSYLEAKRPLDDRSLDRDVLSAFVDALPASPTVLEAGCGTATMPERLFEWGLLDAGTWVAVDSHAAAVTAGRDRLAGRPDATRNGDTVSLGDLTVEFVVADVFEHIGGSRRRFDAVVGNAFFDIVDIERALSTFRPCTDLVYAPITYNGETTVAPPMEPDEQVFARYEEHMRSYRPGHPEAARRLRAALAEPLADAASPWRIEPPYTAAERTVLRHLIETIRTAVGETGLDADAWARQRRAQVADGRLRYEAANRDVLGRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUX1</accession>
    <name>A0A1U7EUX1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">tp35</name>
        <name evidence="10" type="ordered locus">NP_1412A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Potential transporter for phosphate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Membrane</location>
            <topology evidence="2 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322432.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUX1"/>
    <dbReference type="STRING" id="348780.NP_1412A"/>
    <dbReference type="EnsemblBacteria" id="CAI48797">
        <property type="protein sequence ID" value="CAI48797"/>
        <property type="gene ID" value="NP_1412A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703311"/>
    <dbReference type="KEGG" id="nph:NP_1412A"/>
    <dbReference type="eggNOG" id="arCOG02267">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_695609_0_0_2"/>
    <dbReference type="OrthoDB" id="204341at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001204">
        <property type="entry name" value="Phos_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101">
        <property type="entry name" value="PHOSPHATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101:SF80">
        <property type="entry name" value="PHOSPHATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01384">
        <property type="entry name" value="PHO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="136"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="200"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="270"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="313"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="364"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI48797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="388" mass="38357" checksum="9C06005BC6DBCF44" modified="2017-05-10" version="1">MDPLTVVTFVVAGVASLFMAWAIGAGSSGSTPFAPAVGANAISVMRAGFFVGILGLLGATLQGANVTEAVGRELVVGTTLPPIAAILALFIAAALVAVGVFAGYPIATAFTVTGAVVGAGLAVGGDPAWAKYYEILAVWTLTPFVGGGVAYATARLLRQEWLPDVAAIAALAALVGAILANVEFVLLGPADTAQSVSQTIATTGLGVVGVTLFIAAVAAALMGADVRRDAAAGQRHFLIALGALVAFSAGGSQVGLALGPLLPLLDASEVAIPLVALLFGGGLGLLVGSWTAAPRMIKAISQDYSSLGPRRSIAALIPSFIIAQVAILLGVPISFNEIIVSAVVGAGAAAESGGVSRGKMLYTVLAWIGSLVGAGVLSFGLTAVATSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV34</accession>
    <name>A0A1U7EV34_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Molybdopterin-guanine dinucleotide biosynthesis adapter protein MobB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">mobB</name>
        <name evidence="3" type="ordered locus">NP_1536A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322493.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV34"/>
    <dbReference type="STRING" id="348780.NP_1536A"/>
    <dbReference type="EnsemblBacteria" id="CAI48859">
        <property type="protein sequence ID" value="CAI48859"/>
        <property type="gene ID" value="NP_1536A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702931"/>
    <dbReference type="KEGG" id="nph:NP_1536A"/>
    <dbReference type="eggNOG" id="arCOG00532">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068199_3_0_2"/>
    <dbReference type="OrthoDB" id="9014at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052539">
        <property type="entry name" value="MGD_biosynthesis_adapter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004435">
        <property type="entry name" value="MobB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00176">
        <property type="entry name" value="mobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40072:SF1">
        <property type="entry name" value="MOLYBDOPTERIN-GUANINE DINUCLEOTIDE BIOSYNTHESIS ADAPTER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40072">
        <property type="entry name" value="MOLYBDOPTERIN-GUANINE DINUCLEOTIDE BIOSYNTHESIS ADAPTER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03205">
        <property type="entry name" value="MobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Molybdopterin-guanine dinucleotide biosynthesis protein B (MobB)" evidence="2">
        <location>
            <begin position="19"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="168" mass="17237" checksum="7986AA13E6AC1A8F" modified="2017-05-10" version="1">MADADPLVQFADAATPPVVVTVAGPSDSGKTTLVVDLLAAIADDYRVGTVKSIHHDIEPDTPGKDTHKHRTAGAETAVGITPSYTFEVTPGGKADGEAQALAATLSRLAGRGFDLVLVEGFSEAPLPTIRVGDPAARDHAGTVIGTDDDSVAELVDRLEARIKTAGDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV46</accession>
    <name>A0A1U7EV46_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF429 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1574A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322512.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV46"/>
    <dbReference type="STRING" id="348780.NP_1574A"/>
    <dbReference type="EnsemblBacteria" id="CAI48878">
        <property type="protein sequence ID" value="CAI48878"/>
        <property type="gene ID" value="NP_1574A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701190"/>
    <dbReference type="KEGG" id="nph:NP_1574A"/>
    <dbReference type="eggNOG" id="arCOG04711">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080977_1_0_2"/>
    <dbReference type="OrthoDB" id="132880at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007362">
        <property type="entry name" value="DUF429"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04250">
        <property type="entry name" value="DUF429"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="244" mass="26230" checksum="1601AF5855C83E0F" modified="2017-05-10" version="1">MSDHYVGAVVRDGEWLAAAYTESGYDHAAVFEGIGELWTRYEESAVRIAVDAPVGLAADAAPRPNERAARQLLGDRADAIVGAPVREATRKQRYRAAARTHERKTGERLDEAAFERSSTAAAVDAFLDAIDEARPAIVEAHPELCYRAFAGEPMENPPDVAAGYAERMRTLASFERDGPPTVQSVAEATEGHRLPIPAVIDAVALGLTVRPGPGTLRSLPADPPTDDRGLPMRYVYRSATPLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV91</accession>
    <name>A0A1U7EV91_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">IS1341-type transposase ISNph22</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">NP_1696A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322572.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV91"/>
    <dbReference type="STRING" id="348780.NP_1696A"/>
    <dbReference type="EnsemblBacteria" id="CAI48939">
        <property type="protein sequence ID" value="CAI48939"/>
        <property type="gene ID" value="NP_1696A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701227"/>
    <dbReference type="KEGG" id="nph:NP_1696A"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_3_3_2"/>
    <dbReference type="OrthoDB" id="210698at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF26">
        <property type="entry name" value="TRANSPOSASE, PROBABLY IS605-TNPB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="7">
        <location>
            <begin position="178"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="8">
        <location>
            <begin position="298"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="394"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="401"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI48939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="417" mass="47023" checksum="DAACC51E87608C30" modified="2017-05-10" version="1">MEVRRTVPVSLDVDSDDAALLEDTVDTFLWSAQYVVDHAFEGEYVTTSKTTLDDETYDDVREKTDEFNGGLVQAARNKAAEACKSVVARWKNDKKASKPTFTSPHVVYDHRTATFHDDYVSLATTDGRIEADYVLPDEDSDTPHSEYLFSDEYETTGAELHSHDGDWVLHVHCKKEVESDTSEQPTTENGTVLGVDLGVNNLAVASTGTFWTGDEFDHWRREYEKRRGSLQQCGTRWAHENIQSVGRKEEGRFKQMLHRISNELVSEARENECSVIAFEDLTDIRERTGASWGHKWAFNRLYDYVEYKAEEYGISVEQVDPENTSRRCSHCGFTHPDNRDDEDFECLKCGYENHADYNAAKNIGLRYLRRNQTGGDGGAPLGVRLNSGTLNVNGGYSPAEESARTGVHAESHRFSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV97</accession>
    <name>A0A1U7EV97_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PadR family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1678A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322563.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV97"/>
    <dbReference type="STRING" id="348780.NP_1678A"/>
    <dbReference type="EnsemblBacteria" id="CAI48930">
        <property type="protein sequence ID" value="CAI48930"/>
        <property type="gene ID" value="NP_1678A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701221"/>
    <dbReference type="KEGG" id="nph:NP_1678A"/>
    <dbReference type="eggNOG" id="arCOG00004">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_179019_0_0_2"/>
    <dbReference type="OrthoDB" id="224705at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="89" mass="10250" checksum="3831D6E70B6FB73C" modified="2017-05-10" version="1">MEWLTSGLRRDICVLLYAESRRAQQLKSALEARYDRRIPPERFYGAVEALESKGFVETHVEGLEDVYSLTDAGRDGVATQFRWMADHVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVC1</accession>
    <name>A0A1U7EVC1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Heme A synthase</fullName>
            <ecNumber evidence="13">1.3.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ctaA</name>
        <name evidence="13" type="ordered locus">NP_1770A</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="11">Porphyrin-containing compound metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.3.-.-" evidence="13"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322609.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVC1"/>
    <dbReference type="STRING" id="348780.NP_1770A"/>
    <dbReference type="EnsemblBacteria" id="CAI48976">
        <property type="protein sequence ID" value="CAI48976"/>
        <property type="gene ID" value="NP_1770A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701251"/>
    <dbReference type="KEGG" id="nph:NP_1770A"/>
    <dbReference type="eggNOG" id="arCOG03103">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_961751_0_0_2"/>
    <dbReference type="OrthoDB" id="336837at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006784">
        <property type="term" value="P:heme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003780">
        <property type="entry name" value="COX15/CtaA_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050450">
        <property type="entry name" value="COX15/CtaA_HemeA_synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35457">
        <property type="entry name" value="HEME A SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35457:SF1">
        <property type="entry name" value="HEME A SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02628">
        <property type="entry name" value="COX15-CtaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="116"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="158"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="214"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="243"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAI48976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="279" mass="29500" checksum="67E754DFAF889E79" modified="2017-05-10" version="1">MDFRRLLLATTVTTALTALIGVLTATAGAGLTCEARWPLCDGAVFGLFPANFMSFIEWFHRLIAMITGFLIIGSAYAAWRGGAQRRVKLATLVALLLTPLQIVFGAFTVLVQEFVFGYSILVLTLHFGLASVILGLLVAATVWAYGDVTTVTMSKVKQAGVGALLGFPVMVVLTPRLLFTFGEVVQFVYYGLGFAMFSALLVAALWGRELGLRAVPAAAGGASVLIIAQLIVARWAFGDEGQLAILGISFVAFVLTLGVVKQVHDRSQALEGAAAPAGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVD7</accession>
    <name>A0A1U7EVD7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC-type transport system permease protein (Probable substrate phosphate/phosphonate)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">phnE1</name>
        <name evidence="9" type="synonym">abc17p</name>
        <name evidence="9" type="ordered locus">NP_1752A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322600.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVD7"/>
    <dbReference type="STRING" id="348780.NP_1752A"/>
    <dbReference type="EnsemblBacteria" id="CAI48967">
        <property type="protein sequence ID" value="CAI48967"/>
        <property type="gene ID" value="NP_1752A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702421"/>
    <dbReference type="KEGG" id="nph:NP_1752A"/>
    <dbReference type="eggNOG" id="arCOG00173">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064254_0_1_2"/>
    <dbReference type="OrthoDB" id="252910at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015416">
        <property type="term" value="F:ABC-type phosphonate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005769">
        <property type="entry name" value="PhnE/PtxC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01097">
        <property type="entry name" value="PhnE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30043:SF1">
        <property type="entry name" value="ABC TRANSPORT SYSTEM PERMEASE PROTEIN P69"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30043">
        <property type="entry name" value="PHOSPHONATES TRANSPORT SYSTEM PERMEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="90"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="137"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="250"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="86"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI48967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="283" mass="29809" checksum="8F65E80A9731CE2D" modified="2017-05-10" version="1">MTDPDSVDEELARIQRQWQRRMVVRAVAVAAVVAAVVAAGRWLGFDLGYLYAHRQNLQDILLEEMLAPRALWAQLQGDAPTLLPAAVETLAIAGVGTAIGLPLAFALGVLAAGNVTPRYVSTPARLFLGTLRAVPSMVYALLFVILAGLGAVAGTLAIAVGTVGDLGRLFADELEEVDETPAEAVSSAGAGVAATTAAARLPQVTTAYIAWTLFYLELNARKSSVLGIVGAGGIGYPLIMAFRARNYTRVMAAIVVILCLVVGVETLANRLRGWLDAERNASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVH4</accession>
    <name>A0A1U7EVH4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">CobW domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">NP_1822A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SIMIBI class G3E GTPase family. ZNG1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322634.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVH4"/>
    <dbReference type="STRING" id="348780.NP_1822A"/>
    <dbReference type="EnsemblBacteria" id="CAI49002">
        <property type="protein sequence ID" value="CAI49002"/>
        <property type="gene ID" value="NP_1822A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701264"/>
    <dbReference type="KEGG" id="nph:NP_1822A"/>
    <dbReference type="eggNOG" id="arCOG01233">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017452_2_0_2"/>
    <dbReference type="OrthoDB" id="359387at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03112">
        <property type="entry name" value="CobW-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1220.10">
        <property type="entry name" value="CobW-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036627">
        <property type="entry name" value="CobW-likC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011629">
        <property type="entry name" value="CobW-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051927">
        <property type="entry name" value="Zn_Chap_cDPG_Synth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43603">
        <property type="entry name" value="COBW DOMAIN-CONTAINING PROTEIN DDB_G0274527"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43603:SF1">
        <property type="entry name" value="ZINC-REGULATED GTPASE METALLOPROTEIN ACTIVATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07683">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00833">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90002">
        <property type="entry name" value="Hypothetical protein YjiA, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobW C-terminal" evidence="7">
        <location>
            <begin position="267"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="236"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="415" mass="45074" checksum="142B181262671A84" modified="2017-05-10" version="1">MGSGIPVTVLSGGLGAGKTTTLNRLLHESGDRSIAVLVNDMGDINVDAELLASGTDLAADGRVAELSNGCICCELQTDLETEVARLASEQTFDYLVVESSGISEPEPVARLFTTASRAAAAYDLDTMVTVVDASQFVEAFGDSPRLERTAAPGDEERPLSDLLVEQLEFADVVLLNKRDLVDESALDRAETLITALAPRVEIIRTEHGCVDPDDILGTGRFDPVETGSGAGWRQAIDHGDESHAHGGHTHDHDHDHDHIHPESEYGITSFTYRRRRPFDPDELLEQLRTLPSSVVRAKGPFWAAGRDETAFLLSLAGPTVQIEVLGPWLAGLPDIEREMYRNSRPDVSWHDEHGDRRTELVFIGRDIDADALTSQLDACLVADTAQDGTDRQRVLPADDDTLVVDDTHTSGRAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVK8</accession>
    <name>A0A1U7EVK8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ATP-grasp fold protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_1948A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322695.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVK8"/>
    <dbReference type="STRING" id="348780.NP_1948A"/>
    <dbReference type="EnsemblBacteria" id="CAI49065">
        <property type="protein sequence ID" value="CAI49065"/>
        <property type="gene ID" value="NP_1948A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701315"/>
    <dbReference type="KEGG" id="nph:NP_1948A"/>
    <dbReference type="eggNOG" id="arCOG00673">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG01114">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011659_0_0_2"/>
    <dbReference type="OrthoDB" id="3345at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.10">
        <property type="entry name" value="Phosphohistidine domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008279">
        <property type="entry name" value="PEP-util_enz_mobile_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051549">
        <property type="entry name" value="PEP_Utilizing_Enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036637">
        <property type="entry name" value="Phosphohistidine_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002192">
        <property type="entry name" value="PPDK_AMP/ATP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004508">
        <property type="entry name" value="PRK05849.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43615">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43615:SF1">
        <property type="entry name" value="PPDK_N DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00391">
        <property type="entry name" value="PEP-utilizers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01326">
        <property type="entry name" value="PPDK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52009">
        <property type="entry name" value="Phosphohistidine domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate phosphate dikinase AMP/ATP-binding" evidence="2">
        <location>
            <begin position="278"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilising enzyme mobile" evidence="1">
        <location>
            <begin position="961"/>
            <end position="1030"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="1038" mass="115774" checksum="3C18A2BC0FED3300" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVP2</accession>
    <name>A0A1U7EVP2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HD family hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2030A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322735.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVP2"/>
    <dbReference type="STRING" id="348780.NP_2030A"/>
    <dbReference type="EnsemblBacteria" id="CAI49106">
        <property type="protein sequence ID" value="CAI49106"/>
        <property type="gene ID" value="NP_2030A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701332"/>
    <dbReference type="KEGG" id="nph:NP_2030A"/>
    <dbReference type="eggNOG" id="arCOG04430">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026821_3_1_2"/>
    <dbReference type="OrthoDB" id="8895at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008832">
        <property type="term" value="F:dGTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006203">
        <property type="term" value="P:dGTP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3210.10:FF:000037">
        <property type="entry name" value="Metal-dependent phosphohydrolase, HD superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050135">
        <property type="entry name" value="dGTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045509">
        <property type="entry name" value="HD_assoc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373:SF4">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE SAMHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19276">
        <property type="entry name" value="HD_assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD/PDEase" evidence="1">
        <location>
            <begin position="46"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="407" mass="44676" checksum="9DD275D8CF914D95" modified="2017-05-10" version="1">MVTIKDSVHDHITVEGVAADLLDTPAVQRLRRITQLGTVEYVYPSANHTRFEHSLGVYHLATRALDHLGIEGTQAERLRAAAILHDIGHTPYSHNIEALVARRTGKLHDEVEDLLETGDVARTLALHDIDPSAVADLIAGDGEFGPLVSGELDVDRMDYLVRDAHHTGVPYGTIDAGRLIRELRFVDGELVLAEGNVQTAESLLVARALMNPVVYNHHVARISKAMLRRATEQLLTAGVTDAETLRRMDDAALRSALRDCAATAPLATRLDRRTLYKRAVWAELRDVPDDIVDADHETCREHERAIAERAGVDPAAVIIDVPSRPSMRESTSRVVVGGEVRTLGSQSTLVAALRRTQREQWRLGVYAPEAETDAVGSAAEDILGLDLDALVVDVRQGVNTRLDEFQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVU8</accession>
    <name>A0A1U7EVU8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">DUF87 domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">NP_2180A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 11">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HerA family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322809.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVU8"/>
    <dbReference type="STRING" id="348780.NP_2180A"/>
    <dbReference type="EnsemblBacteria" id="CAI49181">
        <property type="protein sequence ID" value="CAI49181"/>
        <property type="gene ID" value="NP_2180A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701371"/>
    <dbReference type="KEGG" id="nph:NP_2180A"/>
    <dbReference type="eggNOG" id="arCOG06224">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016294_1_0_2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
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        <property type="entry name" value="DD_cGKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008571">
        <property type="entry name" value="HerA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002789">
        <property type="entry name" value="HerA_central"/>
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    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
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    <dbReference type="InterPro" id="IPR058885">
        <property type="entry name" value="WHD_halobact"/>
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    <dbReference type="PANTHER" id="PTHR42957">
        <property type="entry name" value="HELICASE MJ1565-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957:SF1">
        <property type="entry name" value="HELICASE MJ1565-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01935">
        <property type="entry name" value="DUF87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26491">
        <property type="entry name" value="WH_Halo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase HerA central" evidence="7">
        <location>
            <begin position="21"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Winged helix" evidence="8">
        <location>
            <begin position="506"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="341"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="625"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="284"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="362"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="402"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="431"/>
            <end position="440"/>
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    </feature>
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        <location>
            <begin position="467"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="490"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="515"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00048954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF26491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI49181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="647" mass="70401" checksum="9E97440D0504A079" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVW2</accession>
    <name>A0A1U7EVW2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Acetolactate synthase</fullName>
            <ecNumber evidence="13">2.2.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">ilvB1</name>
        <name evidence="18" type="ordered locus">NP_2202A</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>2 pyruvate + H(+) = (2S)-2-acetolactate + CO2</text>
            <dbReference type="Rhea" id="RHEA:25249"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="2.2.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="13">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
        <text evidence="13">Binds 1 thiamine pyrophosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 13">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the TPP enzyme family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
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        <property type="protein sequence ID" value="CAI49192"/>
        <property type="gene ID" value="NP_2202A"/>
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    <dbReference type="GeneID" id="3702865"/>
    <dbReference type="KEGG" id="nph:NP_2202A"/>
    <dbReference type="eggNOG" id="arCOG01998">
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    <dbReference type="HOGENOM" id="CLU_013748_1_3_2"/>
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    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00055"/>
    </dbReference>
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        <property type="reaction ID" value="UER00059"/>
    </dbReference>
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    <dbReference type="GO" id="GO:0005948">
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0030976">
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    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
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        <property type="project" value="UniProtKB-UniPathway"/>
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    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
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    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Acetolactate_synth_lsu"/>
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        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
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        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000399">
        <property type="entry name" value="TPP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00118">
        <property type="entry name" value="acolac_lg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968:SF13">
        <property type="entry name" value="ACETOLACTATE SYNTHASE CATALYTIC SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="THIAMINE PYROPHOSPHATE ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00187">
        <property type="entry name" value="TPP_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11 13" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 13" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="6 13 18" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="17">
        <location>
            <begin position="24"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="15">
        <location>
            <begin position="212"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="16">
        <location>
            <begin position="405"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="175"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="179"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAI49192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="581" mass="62095" checksum="79C0BB6B258AB22A" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVW3</accession>
    <name>A0A1U7EVW3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Acetyl-CoA C-acyltransferase</fullName>
            <ecNumber evidence="5">2.3.1.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">acaB8</name>
        <name evidence="5" type="ordered locus">NP_2214A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.16" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322825.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVW3"/>
    <dbReference type="STRING" id="348780.NP_2214A"/>
    <dbReference type="EnsemblBacteria" id="CAI49198">
        <property type="protein sequence ID" value="CAI49198"/>
        <property type="gene ID" value="NP_2214A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702466"/>
    <dbReference type="KEGG" id="nph:NP_2214A"/>
    <dbReference type="eggNOG" id="arCOG01278">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035425_4_0_2"/>
    <dbReference type="OrthoDB" id="167534at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003988">
        <property type="term" value="F:acetyl-CoA C-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00829">
        <property type="entry name" value="SCP-x_thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055140">
        <property type="entry name" value="Thiolase_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870">
        <property type="entry name" value="ACETYL-COA C-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870:SF6">
        <property type="entry name" value="ACETYL-COA C-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22691">
        <property type="entry name" value="Thiolase_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="1" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="3">
        <location>
            <begin position="5"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="4">
        <location>
            <begin position="243"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="311"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="312"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="388" mass="40195" checksum="2DE0F115AB131C40" modified="2017-05-10" version="1">MSRAAVVGAGMTKFGVHESPMQELFADAAFEALDDAGIEAGEIEALYFGNAMGGQTENETHLGPKMATHIGMAGTPVQRFEDACATSANAFKNAVQAVEAGVHDAVLVGGVERCTPETGKDTPEMTRIFGSASHRQYEQPSGLTFPGVFALLTKRHMHEHGTTEEQLAHVAVKNHGNGALNPNAHFGKETTVEEALDGPIVADPFRLMDCCPFSDGASAVVVVSDDLADSYDAPVDVTGVGHATDVVPIGDKREIGVTQAARDAAAEAYEQAGIDADAVDFAEVHDCFTGAEILASEALGLIEDGEGGVAAEEGRTARDGDIPINPSGGLKAKGHPIGATGTAQIVELTKHLRGEAGERQLDAADCGVAHNLGGDSATTVVSVMEARQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW02</accession>
    <name>A0A1U7EW02_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADH dehydrogenase-like complex subunit J2</fullName>
            <ecNumber evidence="2">1.6.5.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">nuoJ2</name>
        <name evidence="2" type="ordered locus">NP_2306A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.6.5.-" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322871.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW02"/>
    <dbReference type="STRING" id="348780.NP_2306A"/>
    <dbReference type="EnsemblBacteria" id="CAI49244">
        <property type="protein sequence ID" value="CAI49244"/>
        <property type="gene ID" value="NP_2306A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702995"/>
    <dbReference type="KEGG" id="nph:NP_2306A"/>
    <dbReference type="eggNOG" id="arCOG07914">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143951_0_0_2"/>
    <dbReference type="OrthoDB" id="214784at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1200">
        <property type="entry name" value="NADH-ubiquinone/plastoquinone oxidoreductase chain 6, subunit NuoJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042106">
        <property type="entry name" value="Nuo/plastoQ_OxRdtase_6_NuoJ"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="93" mass="9535" checksum="4CBC4C7D56DE7093" modified="2017-05-10" version="1">MTTRPRLYRGSKLAGVLAVGLFAFLAAVFLTAGFGTSGAFPEGSVTESIGYAMFDLPGGDIDSEGFLVSFILIALVLDAALDGAIMLAKGGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW49</accession>
    <name>A0A1U7EW49_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2444A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49313.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322939.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW49"/>
    <dbReference type="STRING" id="348780.NP_2444A"/>
    <dbReference type="EnsemblBacteria" id="CAI49313">
        <property type="protein sequence ID" value="CAI49313"/>
        <property type="gene ID" value="NP_2444A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701446"/>
    <dbReference type="KEGG" id="nph:NP_2444A"/>
    <dbReference type="eggNOG" id="arCOG04547">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104052_0_0_2"/>
    <dbReference type="OrthoDB" id="308076at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055968">
        <property type="entry name" value="DUF7546"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24412">
        <property type="entry name" value="DUF7546"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49313.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="221" mass="22921" checksum="00141791222CEE07" modified="2017-05-10" version="1">MTATDKDPNWTLIYTAIVANALVLAALGYALLAERQPTAYWAVPLVWITVGVWALLRVSPPPAARRTKALAGAVAGGYFLVLGFVGGLFGPASGPTTGLLIQATELPPGWNPAVLYAGEYLQFAIIPYTAFGYGVLAYLIYVTAVEAKHAVAGGLLGLFSCVSCTLPVIAALVGGVVGGGGAVAQAAIEFQSYAISTAVFVVTVGLLSYRPGLGQFRRWLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW55</accession>
    <name>A0A1U7EW55_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UspA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2380A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322907.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW55"/>
    <dbReference type="STRING" id="348780.NP_2380A"/>
    <dbReference type="EnsemblBacteria" id="CAI49281">
        <property type="protein sequence ID" value="CAI49281"/>
        <property type="gene ID" value="NP_2380A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701431"/>
    <dbReference type="KEGG" id="nph:NP_2380A"/>
    <dbReference type="eggNOG" id="arCOG00451">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101252_0_0_2"/>
    <dbReference type="OrthoDB" id="157328at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="1">
        <location>
            <begin position="135"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="241" mass="25416" checksum="499117A6CD889549" modified="2017-05-10" version="1">MTSTPPTIVVPVDVRGRESVPETLAAFLSPLPVLVVGVVEIPDQTAPKQARDQFEDDARQTLETVAETFAAAGAAVETRLTFTHDSQRTIEQVATDIDRVAILFPAPATAAESVLVAIRGGINVPNIAATVAALLRSTDASATLYHAATPDADTAEAEQALRALGSALVEAGLDEARFRTVIEADDAPLSALVAAANEHDLLVVGEDEPTIRDRIFGDTSERVAEQTAVPVVIVRRPPVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW64</accession>
    <name>A0A1U7EW64_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Probable secreted glycoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2468A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322951.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW64"/>
    <dbReference type="EnsemblBacteria" id="CAI49325">
        <property type="protein sequence ID" value="CAI49325"/>
        <property type="gene ID" value="NP_2468A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701454"/>
    <dbReference type="KEGG" id="nph:NP_2468A"/>
    <dbReference type="eggNOG" id="arCOG06273">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_308112_0_0_2"/>
    <dbReference type="OrthoDB" id="232948at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045517">
        <property type="entry name" value="halo_surf_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="921" mass="98819" checksum="C6262E056CE7F830" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWA7</accession>
    <name>A0A1U7EWA7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">GNAT family acetyltransferase</fullName>
            <ecNumber evidence="4">2.3.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_2494A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322964.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWA7"/>
    <dbReference type="STRING" id="348780.NP_2494A"/>
    <dbReference type="EnsemblBacteria" id="CAI49338">
        <property type="protein sequence ID" value="CAI49338"/>
        <property type="gene ID" value="NP_2494A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701464"/>
    <dbReference type="KEGG" id="nph:NP_2494A"/>
    <dbReference type="eggNOG" id="arCOG00845">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_907952_0_0_2"/>
    <dbReference type="OrthoDB" id="134118at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050832">
        <property type="entry name" value="Bact_Acetyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43877:SF2">
        <property type="entry name" value="AMINOALKYLPHOSPHONATE N-ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43877">
        <property type="entry name" value="AMINOALKYLPHOSPHONATE N-ACETYLTRANSFERASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="4"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="299" mass="32540" checksum="CDF032D1ABF2203E" modified="2017-05-10" version="1">MADIAVRPAQADDYEAVAAFTADTWQRLDGDYIPDVFDEWVASDGPTQRTLVATVEDRPVGLCQGVLLSEDEAWAQGMRVDPEYRGRDIARRLVTGVFEWAADAGATVCRNMVFSWNEAGLGLSRSVGFEPLASFRWLEPTPDADADCGLAVDSTPDAAWRGYRRSDACRRLYGLGLDTDEPWALAELTRERLAAVERPLVVADDDHIRGVSYRVRTTDEETDDGESVSRAEYGVGAWNDAEALSALSAAVARDAAAAGADRTRLLVPETPRHISDAAAVGVAAADAPDFVLERELTRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWB1</accession>
    <name>A0A1U7EWB1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UspA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2560A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322996.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWB1"/>
    <dbReference type="STRING" id="348780.NP_2560A"/>
    <dbReference type="EnsemblBacteria" id="CAI49371">
        <property type="protein sequence ID" value="CAI49371"/>
        <property type="gene ID" value="NP_2560A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701487"/>
    <dbReference type="KEGG" id="nph:NP_2560A"/>
    <dbReference type="eggNOG" id="arCOG02053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_11_1_2"/>
    <dbReference type="OrthoDB" id="105697at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF6">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN UP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="143" mass="15260" checksum="F95CEDE2F221EEB8" modified="2017-05-10" version="1">MYDSILLPTDGSENASEALSHAIEAANSYDADLHIVSVVDERVILAAAPEDKEEVREDLAAEANDAVEELTARATDAGLDVTAATPSGVPYREILSYADDEAVDLLVLGTHGRTGREKRLNLGSTTERVIKDGDRPVLVVDIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWG9</accession>
    <name>A0A1U7EWG9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2706A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323069.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWG9"/>
    <dbReference type="STRING" id="348780.NP_2706A"/>
    <dbReference type="EnsemblBacteria" id="CAI49444">
        <property type="protein sequence ID" value="CAI49444"/>
        <property type="gene ID" value="NP_2706A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701532"/>
    <dbReference type="KEGG" id="nph:NP_2706A"/>
    <dbReference type="eggNOG" id="arCOG07597">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2420135_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="91" mass="9689" checksum="290E28BA5810CDDD" modified="2017-05-10" version="1">MSIPYASPIAPIADSPDDPRPMTDGGDTKSYRPPSDAPLVPIADEPATPTTQPRADGGDRSKRFARPPETDHASGYDTGQETILPLVADMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWH6</accession>
    <name>A0A1U7EWH6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DUF389 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2674A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323053.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWH6"/>
    <dbReference type="STRING" id="348780.NP_2674A"/>
    <dbReference type="EnsemblBacteria" id="CAI49428">
        <property type="protein sequence ID" value="CAI49428"/>
        <property type="gene ID" value="NP_2674A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701521"/>
    <dbReference type="KEGG" id="nph:NP_2674A"/>
    <dbReference type="eggNOG" id="arCOG02264">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050976_1_1_2"/>
    <dbReference type="OrthoDB" id="3266at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005240">
        <property type="entry name" value="DUF389"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00341">
        <property type="entry name" value="TIGR00341 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20992">
        <property type="entry name" value="AT15442P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20992:SF9">
        <property type="entry name" value="AT15442P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04087">
        <property type="entry name" value="DUF389"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="139"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="241"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="270"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="315"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="418" mass="44442" checksum="16D58CCFE5BB8B4C" modified="2017-05-10" version="1">MRLVQTLVPEGKHDVVVEALEEADVDYAMADETSRSDYSDIVFVPADADDVETILEALRDIGVERAGYTMVSEVETIASERFERQQDAGDEQGGDERISRDELRTKARNLSRSTPNYLAFTIISAVVATAGLLEDSASIVVGSMVIAPLIGPAMASCVGTVINDDDLFWEGIRSQAIGVVLAVGASTAFAFVYRLGVRPELDLLLLGQVAERAHPGILALAVALGAGAAGALSLTSGADEALVGVMIAVALMPPAASVGLGVAYTDWVLAVGASILVAVNLLSINAAGIVTFWTKRYRPSHWYDEARARRATFERFAAFVVAILILTSFLAVASQEARANAAFESTVEDIADEVVDGDVRSVTVHYDPDFVSPTPASVVVHADAERNGVAESLRQTILDRTGHDIDITVVYETTESAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWK9</accession>
    <name>A0A1U7EWK9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">OsmC domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2768A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323100.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWK9"/>
    <dbReference type="STRING" id="348780.NP_2768A"/>
    <dbReference type="EnsemblBacteria" id="CAI49475">
        <property type="protein sequence ID" value="CAI49475"/>
        <property type="gene ID" value="NP_2768A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701551"/>
    <dbReference type="KEGG" id="nph:NP_2768A"/>
    <dbReference type="eggNOG" id="arCOG03687">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1912282_0_0_2"/>
    <dbReference type="OrthoDB" id="237916at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003718">
        <property type="entry name" value="OsmC/Ohr_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036102">
        <property type="entry name" value="OsmC/Ohrsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34352">
        <property type="entry name" value="PROTEIN YHFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34352:SF1">
        <property type="entry name" value="PROTEIN YHFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02566">
        <property type="entry name" value="OsmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82784">
        <property type="entry name" value="OsmC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="132" mass="14379" checksum="A09E866F95DCCC0F" modified="2017-05-10" version="1">MSDIETTTINEEKYHAFSRAGEFELSIDASGNDGPSPNEVLVADYASCFTFACRAGAKRRLEVDLGKVETEAEADLDDSDDIEAIRFHLFIEAALTDEQLESVIELGEEICHVHAVLDSAVHADVEATANAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWQ2</accession>
    <name>A0A1U7EWQ2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2870A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323151.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWQ2"/>
    <dbReference type="STRING" id="348780.NP_2870A"/>
    <dbReference type="EnsemblBacteria" id="CAI49526">
        <property type="protein sequence ID" value="CAI49526"/>
        <property type="gene ID" value="NP_2870A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701577"/>
    <dbReference type="KEGG" id="nph:NP_2870A"/>
    <dbReference type="eggNOG" id="arCOG00504">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030571_2_4_2"/>
    <dbReference type="OrthoDB" id="197151at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07721">
        <property type="entry name" value="yflN-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050855">
        <property type="entry name" value="NDM-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42951:SF4">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE MBLAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42951">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="21"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="232" mass="25986" checksum="776AA65ED335F55D" modified="2017-05-10" version="1">MARAVTDGVYRLDIAWPEPVGANAYLVDDGEVTLVDAGVPFPRRSLSGELRSLGYSLDDIDRVLVTHYDIDHVGGLARIDIDVPVYIGALDRRLVRRAWSPPWRHHKGVFHRVVRRWYSLSDYDLRPVEDDDTIGAFRTFHTPGHNPGHTVYIHDETETAMLGDLVWATDGGFVPPPWLDSYDTARLAESIARVAQESFAHACVGHGPVVSPDAAERLRSLVVRRFGADRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWQ6</accession>
    <name>A0A1U7EWQ6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2888A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323160.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWQ6"/>
    <dbReference type="STRING" id="348780.NP_2888A"/>
    <dbReference type="EnsemblBacteria" id="CAI49535">
        <property type="protein sequence ID" value="CAI49535"/>
        <property type="gene ID" value="NP_2888A"/>
    </dbReference>
    <dbReference type="GeneID" id="77383463"/>
    <dbReference type="KEGG" id="nph:NP_2888A"/>
    <dbReference type="eggNOG" id="arCOG06262">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1691590_0_0_2"/>
    <dbReference type="OrthoDB" id="205286at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045396">
        <property type="entry name" value="DUF6517"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20127">
        <property type="entry name" value="DUF6517"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="155" mass="17217" checksum="90516F818544F772" modified="2017-05-10" version="1">MGTEELTELLEADYDDISNVEHVEDDELTILEQETTVARYEADAMFEGQELPVYIHISESVETSNDHLVTIGVYPQQVRQQEDPNIEALMTGVIEEADETADDAEDNRDDADDADDEPAEDGSLPEDEDTVDELPEDENLSDDLPDGVDVDISLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWU2</accession>
    <name>A0A1U7EWU2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PHP domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2980A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323205.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWU2"/>
    <dbReference type="STRING" id="348780.NP_2980A"/>
    <dbReference type="EnsemblBacteria" id="CAI49581">
        <property type="protein sequence ID" value="CAI49581"/>
        <property type="gene ID" value="NP_2980A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701614"/>
    <dbReference type="KEGG" id="nph:NP_2980A"/>
    <dbReference type="eggNOG" id="arCOG00302">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072983_3_0_2"/>
    <dbReference type="OrthoDB" id="50465at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07432">
        <property type="entry name" value="PHP_HisPPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000101">
        <property type="entry name" value="PHP domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004013">
        <property type="entry name" value="PHP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052018">
        <property type="entry name" value="PHP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003141">
        <property type="entry name" value="Pol/His_phosphatase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038032">
        <property type="entry name" value="CehA_McbA_metalo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42924">
        <property type="entry name" value="EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42924:SF3">
        <property type="entry name" value="POLYMERASE_HISTIDINOL PHOSPHATASE N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02811">
        <property type="entry name" value="PHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13263">
        <property type="entry name" value="PHP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00481">
        <property type="entry name" value="POLIIIAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="PHP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polymerase/histidinol phosphatase N-terminal" evidence="1">
        <location>
            <begin position="4"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="225" mass="23816" checksum="739838F6AE9F2BE8" modified="2017-05-10" version="1">MLSVELHAHSSLSYDGRDPVELLLEQAEAVGLDALAVTDHDEIDASLAAASKAADYGLVGIPGMEVSSAAGHILALGVEELVPAGLSYDETLDRIRDQGGIAVVPHPFQTSRHGVAPHITDAQLAAADAIEVYNSRLFTGRANRQAERFAERYDVPMTAGSDAHIAEMVGQAVTEVDAYHRSAEGILDAIAEGRTSVVGKRTPWAISIQQFGGGVTRRIAGLLPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX01</accession>
    <name>A0A1U7EX01_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3094A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX01"/>
    <dbReference type="STRING" id="348780.NP_3094A"/>
    <dbReference type="EnsemblBacteria" id="CAI49638">
        <property type="protein sequence ID" value="CAI49638"/>
        <property type="gene ID" value="NP_3094A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3094A"/>
    <dbReference type="HOGENOM" id="CLU_3210846_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="44" mass="5236" checksum="AAD154C51C2C176E" modified="2017-05-10" version="1">MQGKQSGSKMEQKDEEDTKELKGQKRKTKTASYETPETDERYFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX17</accession>
    <name>A0A1U7EX17_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UspA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3144A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323285.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX17"/>
    <dbReference type="STRING" id="348780.NP_3144A"/>
    <dbReference type="EnsemblBacteria" id="CAI49663">
        <property type="protein sequence ID" value="CAI49663"/>
        <property type="gene ID" value="NP_3144A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701667"/>
    <dbReference type="KEGG" id="nph:NP_3144A"/>
    <dbReference type="eggNOG" id="arCOG02053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_11_0_2"/>
    <dbReference type="OrthoDB" id="105697at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF6">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN UP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="143" mass="15087" checksum="A3A83F1941A063C2" modified="2017-05-10" version="1">MYDRILVPTDGSEGAERAIEHALSLAEVHGATVHGLYAVEPVYSTETGTAEAVAALEDAGERAVASLAEAAEARNVAAVTDVRRGTPHQMILDYAEEEGIDVIVMGTEGRTGLSRYLVGSVAEKVVRLSDVPVLTVRAEPAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX20</accession>
    <name>A0A1U7EX20_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Metal-dependent hydrolase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3152A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323289.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX20"/>
    <dbReference type="STRING" id="348780.NP_3152A"/>
    <dbReference type="EnsemblBacteria" id="CAI49667">
        <property type="protein sequence ID" value="CAI49667"/>
        <property type="gene ID" value="NP_3152A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701671"/>
    <dbReference type="KEGG" id="nph:NP_3152A"/>
    <dbReference type="eggNOG" id="arCOG00515">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020884_3_0_2"/>
    <dbReference type="OrthoDB" id="336381at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050114">
        <property type="entry name" value="UPF0173_UPF0282_UlaG_hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43546:SF9">
        <property type="entry name" value="L-ASCORBATE-6-PHOSPHATE LACTONASE ULAG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43546">
        <property type="entry name" value="UPF0173 METAL-DEPENDENT HYDROLASE MJ1163-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="2">
        <location>
            <begin position="41"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="275" mass="29767" checksum="B24C480D879E1B1F" modified="2017-05-10" version="1">MTIHSDWGDWLPTAIAAAEPDGLAVWYLGCNGFALKAADGTTVFIDPYLGTGDPPRTVRMIPVPFEPTDVVEADAVLATHDHTDHTHGPSQAPILTGTDATFYGPPTSVATAEAWTDEHGVDGNDIAEITEGETLEVGSLRIHVEPAHDPDADHPVGYVVEHESGTFFHGGDARPSEDFEAIGGSYDIDLGAVAFGSAGMIPDKETREPKYTQWYSNENMAAEAAAQLQLDRLLPTHWDMWKGLTADPTALRDHTRSFDHPRTLEILEIGDRTSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX66</accession>
    <name>A0A1U7EX66_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Probable S-adenosylmethionine-dependent methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3360A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323391.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX66"/>
    <dbReference type="STRING" id="348780.NP_3360A"/>
    <dbReference type="EnsemblBacteria" id="CAI49771">
        <property type="protein sequence ID" value="CAI49771"/>
        <property type="gene ID" value="NP_3360A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701748"/>
    <dbReference type="KEGG" id="nph:NP_3360A"/>
    <dbReference type="eggNOG" id="arCOG04580">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_559761_0_0_2"/>
    <dbReference type="OrthoDB" id="117536at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058959">
        <property type="entry name" value="DUF8157_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058470">
        <property type="entry name" value="DUF8157_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26486">
        <property type="entry name" value="DUF8157"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26487">
        <property type="entry name" value="DUF8157_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13489">
        <property type="entry name" value="Methyltransf_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DUF8157" evidence="1">
        <location>
            <begin position="5"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="DUF8157" evidence="2">
        <location>
            <begin position="382"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="475" mass="52876" checksum="5F2B2D1AAB108EC0" modified="2017-05-10" version="1">MTDTEAVLDTARYLREVRPIDPEEIYEYVDGQPHPAVVRQTLREHAFELGLKEREDGAFVPVEDGTVHPDFTGVERFPEQYARQLESLLVDRYGAGWPDSDAGDRLREHIDQLKVDYFADADVTYDEETALAYALYHLPDYYAAIQYVLDDLGSAGLLGRRLRVLDVGAGTGGPMLGIHEYLPEETLVDYDAVEPSAAADVFEQMASETRRGFEPTLYRETAESFSPDGDYDLIVFANVLSELSQPAAVFERYLDHLADDGTVVAVSPAEERTATRLRDIEREVLDRRPDATVYAPTIRLWPDESPSDRGWTFTRQADIEPPAFQTRLDAAAADDAAADGIAAGGDGTYTKTTVQYAYLLLRTDGRRAIEYTPDPDTVAKMADMDAHVTDRIDLAALKLSPDLSSDGNPLYKISDGSEAVDHYAVLTRESSLNRELPAAPYGSLLRFENVLVLWNDDEDAYNLVVDDETVVDRLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX70</accession>
    <name>A0A1U7EX70_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CRISPR-associated endonuclease Cas1</fullName>
            <ecNumber evidence="9">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">cas1</name>
        <name evidence="10" type="ordered locus">NP_3274A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as a dsDNA endonuclease. Involved in the integration of spacer DNA into the CRISPR cassette.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer, forms a heterotetramer with a Cas2 homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the CRISPR-associated endonuclease Cas1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="9"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323349.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX70"/>
    <dbReference type="STRING" id="348780.NP_3274A"/>
    <dbReference type="EnsemblBacteria" id="CAI49728">
        <property type="protein sequence ID" value="CAI49728"/>
        <property type="gene ID" value="NP_3274A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701712"/>
    <dbReference type="KEGG" id="nph:NP_3274A"/>
    <dbReference type="eggNOG" id="arCOG01452">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052779_1_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004520">
        <property type="term" value="F:DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09634">
        <property type="entry name" value="Cas1_I-II-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.920">
        <property type="entry name" value="CRISPR-associated endonuclease Cas1, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.20">
        <property type="entry name" value="CRISPR-associated endonuclease Cas1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01470">
        <property type="entry name" value="Cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050646">
        <property type="entry name" value="Cas1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002729">
        <property type="entry name" value="CRISPR-assoc_Cas1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042206">
        <property type="entry name" value="CRISPR-assoc_Cas1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023843">
        <property type="entry name" value="CRISPR-assoc_Cas1_cyanobact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042211">
        <property type="entry name" value="CRISPR-assoc_Cas1_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00287">
        <property type="entry name" value="cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04093">
        <property type="entry name" value="cas1_CYANO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34353">
        <property type="entry name" value="CRISPR-ASSOCIATED ENDONUCLEASE CAS1 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34353:SF2">
        <property type="entry name" value="CRISPR-ASSOCIATED ENDONUCLEASE CAS1 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01867">
        <property type="entry name" value="Cas_Cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="7 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 9 10" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4 9 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 9" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI49728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="331" mass="38009" checksum="B9D01947486F2C2B" modified="2017-05-10" version="1">MKAAEGMFDESVVYVTKQGTQVRTDGGQIIVWDVDGDDGELAAYPKEKLDTINVFGGVNFSTPFVAEANEHGIVLNYFTQNGKYRGSFVPEKNTIAEVRRAQYALTPAKELAIAKEMIRGKIRNQRTLLSRKGVTGTDVLKDLGVRVDNIKTKDDLRGAEGEAAERYFDRLDETLVDGWTFEKRTKRPPEDHINSLLSLTYVFMKNEVMSALRQYNLDPFLGVLHADRHGRPSLALDLQEEFRPIFCDALVTRLVNRRTLTHDDFNKDNRLSDDAFKTYLSKFDEFMQEEFTHPHFEYSVTRRKAVRQQAILLRKAITGEMDQYHPLTFQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXD6</accession>
    <name>A0A1U7EXD6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">GalE family epimerase/dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3504A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323463.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXD6"/>
    <dbReference type="STRING" id="348780.NP_3504A"/>
    <dbReference type="EnsemblBacteria" id="CAI49843">
        <property type="protein sequence ID" value="CAI49843"/>
        <property type="gene ID" value="NP_3504A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702736"/>
    <dbReference type="KEGG" id="nph:NP_3504A"/>
    <dbReference type="eggNOG" id="arCOG01369">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_7_2"/>
    <dbReference type="OrthoDB" id="4907at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43000">
        <property type="entry name" value="DTDP-D-GLUCOSE 4,6-DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="2">
        <location>
            <begin position="6"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="305" mass="32968" checksum="71388E55232105EF" modified="2017-05-10" version="1">MDGQRVLVTGGAGFIGSTLANRLSVTNDVVVVDDCYLGTPENLESAVTFNERSVLDDDLPTDVDVVFHCAALSSYPMHESDPTRGARVNVEGFVNVVEQARADGCDTVVYASSSSVYGSRSEPTTEAAPVAANSGYEASKLARERYAEYFSHHYGLSLAGMRLFSVYQGYGAAEGHKGEYANLVAQFVDDIATGERPAIYGDGTQTRDFIHVEDVVAALCSAAEAALDGVYNVGTGDRYSLNDLVDKINETVGADIRPKYVENPIPEDVYVTDTCADCSKLRSETNWEPTVGFDEGVKRVCEQYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXM9</accession>
    <name>A0A1U7EXM9_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Methionine synthase</fullName>
            <ecNumber evidence="8">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Homocysteine methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">metE1</name>
        <name evidence="8" type="synonym">metE</name>
        <name evidence="10" type="ordered locus">NP_3670A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the transfer of a methyl group to L-homocysteine resulting in methionine formation. The physiological methyl donor is unknown.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the archaeal MetE family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="8"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323544.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXM9"/>
    <dbReference type="STRING" id="348780.NP_3670A"/>
    <dbReference type="EnsemblBacteria" id="CAI49926">
        <property type="protein sequence ID" value="CAI49926"/>
        <property type="gene ID" value="NP_3670A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702917"/>
    <dbReference type="KEGG" id="nph:NP_3670A"/>
    <dbReference type="eggNOG" id="arCOG01876">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040013_3_2_2"/>
    <dbReference type="OrthoDB" id="17656at2157"/>
    <dbReference type="UniPathway" id="UPA00051"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003871">
        <property type="term" value="F:5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03311">
        <property type="entry name" value="CIMS_C_terminal_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00288">
        <property type="entry name" value="MetE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002629">
        <property type="entry name" value="Met_Synth_C/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022921">
        <property type="entry name" value="MetE_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003317">
        <property type="entry name" value="PRK04326.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30519">
        <property type="entry name" value="5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMOCYSTEINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01717">
        <property type="entry name" value="Meth_synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="UROD/MetE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="2 8 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Cobalamin-independent methionine synthase MetE C-terminal/archaeal" evidence="9">
        <location>
            <begin position="25"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI49926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="356" mass="40476" checksum="7B790C74F16A700A" modified="2017-05-10" version="1">MSNRSLPETRAQFRPKDHPTEHFLLTTVVGSYPKPKWLNRAKELAEDDDRDFDDDDLEEALDDAARLITEEHTRAGLDAVVDGEMRRNEMVEYFADRIDGYEFNGPVKVWGHNYFDKPSVADEVAYDEPWLVDEFEFTTGVTERPVKVPITGPYTLASWSFNEHYEGEAELAYELADLVNEEINRLVEAGCRYIQIDEPALATTPDDHAIVGECLEHIADGIPEDVRLGLHVCYGDYSRIYPEILDMPVHEYDLELANGDYEQLDVFKEPTFEKDLALGVVDVHTTDVETVEEIKENIIKGFEIVPPERLTVSPDCGVKLLPREVAYQKMENMVEAARQVEKELDAGEITVGYAGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXQ1</accession>
    <name>A0A1U7EXQ1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NADPH:quinone reductase</fullName>
            <ecNumber evidence="4">1.6.5.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">qor2</name>
        <name evidence="4" type="ordered locus">NP_3766A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.6.5.5" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323591.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXQ1"/>
    <dbReference type="STRING" id="348780.NP_3766A"/>
    <dbReference type="EnsemblBacteria" id="CAI49974">
        <property type="protein sequence ID" value="CAI49974"/>
        <property type="gene ID" value="NP_3766A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703091"/>
    <dbReference type="KEGG" id="nph:NP_3766A"/>
    <dbReference type="eggNOG" id="arCOG01458">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_3_1_2"/>
    <dbReference type="OrthoDB" id="146629at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003960">
        <property type="term" value="F:quinone reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08241">
        <property type="entry name" value="QOR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48106">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE PIG3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="3">
        <location>
            <begin position="10"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="323" mass="33940" checksum="27D8B645255F6F28" modified="2017-05-10" version="1">MKAATVTEYGEADVLEVTDRERPRPGEGEVLIEVEAAGINFADIMQRRGHYQGGPSPEYVPGMEVAGTIAEVGDGVNREAGEAVVSLVNGGGYAEYATADARGLLDIPGDLSFEEAAGFPVQWLTAHNCLHEWGGLEADETVLVHAAAGGVGSAAVQLADEAGAAVIGTASTEEKLSMASDLGMDHGIQYTEEDFVDRVNEITDGDGVDLVLDGIGSDTSDRSLQALRSFGRMVSYGAAGGEPGRPNTADLLFSNQRVIGYHLGRAIEQKPMKVMGAVPELTQLLGEGTLEVQVGHTFDLEEAASAHQFIEDRKSSGKVVLVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXR0</accession>
    <name>A0A1U7EXR0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glutaredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">grx3</name>
        <name evidence="2" type="ordered locus">NP_3758A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323587.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXR0"/>
    <dbReference type="STRING" id="348780.NP_3758A"/>
    <dbReference type="EnsemblBacteria" id="CAI49970">
        <property type="protein sequence ID" value="CAI49970"/>
        <property type="gene ID" value="NP_3758A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702764"/>
    <dbReference type="KEGG" id="nph:NP_3758A"/>
    <dbReference type="eggNOG" id="arCOG02608">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026126_8_2_2"/>
    <dbReference type="OrthoDB" id="73564at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011767">
        <property type="entry name" value="GLR_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45288">
        <property type="entry name" value="THIOREDOXIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45288:SF1">
        <property type="entry name" value="THIOREDOXIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI49970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="91" mass="10322" checksum="02D0D9E58110019C" modified="2017-05-10" version="1">MTLTLYRLEGCPYCEFVVDTLEDLPLDFESVWVEGLHSKRNEVHEITGQRQVPALVDDAHGVSMSQSARIIEYLETTYGDATSPDEVELDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY01</accession>
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        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
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            </authorList>
            <dbReference type="PubMed" id="16169924"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
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        </location>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01951"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI50084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="137" mass="14902" checksum="BBCECD0E5E27D376" modified="2017-05-10" version="1">MGYELRDHTADIAVAATGATLDAVFAAVADGLSAAHCETWPDDRGDRVSVAAAAESREALLFEYLAELIYQRDVRGVLPVDHELAVEEDAGDWQLEGSARGVPLDAITAREIKAVTYSEMELTETEDGWRAYVVFDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY13</accession>
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        <submittedName>
            <fullName evidence="3">Small CPxCG-related zinc finger protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ordered locus">NP_4000A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
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            <taxon>Methanobacteriati</taxon>
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            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    <dbReference type="EnsemblBacteria" id="CAI50091">
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        <property type="gene ID" value="NP_4000A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701935"/>
    <dbReference type="KEGG" id="nph:NP_4000A"/>
    <dbReference type="eggNOG" id="arCOG07555">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2461861_0_0_2"/>
    <dbReference type="OrthoDB" id="209680at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058419">
        <property type="entry name" value="DUF8106"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26408">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="61"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="DUF8106" evidence="2">
        <location>
            <begin position="6"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="88" mass="9480" checksum="DBA4D039443E68B7" modified="2017-05-10" version="1">MMSPDKKSTLFCPDCDHYGPMATDWSRSRRDGREELECPDCGTVVVSQPQFGTADDGRTRVALAACAGLATLLAGLVQLAGFFSVHSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY34</accession>
    <name>A0A1U7EY34_NATPD</name>
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        <submittedName>
            <fullName evidence="3">DUF2339 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_4038A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY34"/>
    <dbReference type="STRING" id="348780.NP_4038A"/>
    <dbReference type="EnsemblBacteria" id="CAI50110">
        <property type="protein sequence ID" value="CAI50110"/>
        <property type="gene ID" value="NP_4038A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701950"/>
    <dbReference type="KEGG" id="nph:NP_4038A"/>
    <dbReference type="eggNOG" id="arCOG06890">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_443873_0_0_2"/>
    <dbReference type="OrthoDB" id="222435at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019286">
        <property type="entry name" value="DUF2339_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38434">
        <property type="entry name" value="BLL2549 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38434:SF1">
        <property type="entry name" value="BLL2549 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10101">
        <property type="entry name" value="DUF2339"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="204"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="246"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
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            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="301"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="334"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="365"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="448"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="479"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="504"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="530"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="558"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="586" mass="60352" checksum="FE29373B4FDBF368" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYA6</accession>
    <name>A0A1U7EYA6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Major facilitator superfamily transport protein (Probable substrate nitrate/nitrite)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">nasB</name>
        <name evidence="9" type="synonym">narK:tp42</name>
        <name evidence="9" type="ordered locus">NP_4228A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323821.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYA6"/>
    <dbReference type="STRING" id="348780.NP_4228A"/>
    <dbReference type="EnsemblBacteria" id="CAI50205">
        <property type="protein sequence ID" value="CAI50205"/>
        <property type="gene ID" value="NP_4228A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703320"/>
    <dbReference type="KEGG" id="nph:NP_4228A"/>
    <dbReference type="eggNOG" id="arCOG00148">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024204_2_0_2"/>
    <dbReference type="OrthoDB" id="341844at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015112">
        <property type="term" value="F:nitrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042128">
        <property type="term" value="P:nitrate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044772">
        <property type="entry name" value="NO3_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23515">
        <property type="entry name" value="HIGH-AFFINITY NITRATE TRANSPORTER 2.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0534">Nitrate assimilation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="230"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="266"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="312"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="337"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="372"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="402"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="1"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI50205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="452" mass="48124" checksum="720A61E3F14ECCC2" modified="2017-05-10" version="1">MAITDLLYGKYRNLLMATAMFNLGFVIWFSFAPYTGEIATEFGLSVADLGIVASAAIVAVPLGRIVIGPLTDRFGAHVTGSVTLITVGIFAVISAFATTYAVFTGSRIIASLSGITFVIGIQHVAEWFEEENLGTAEGIFAGIGNAGAGLGAYFTLPRIFGEGYIDPIFGPEFLATSANWRAAFFYTGLIAIVLGIAYYVLCDAAKSEAKRNATKAGVSWDQWKFIVTRYGAVVLSVAYIMTFGLELAMNGWLGTYYREAFGQGDIVIAATFAATFSIAAGLLRPLGGYISDLVKRKEMNLLPWFHGEYRNQWTFVTLVYVMLTMFGMTAAGLTGNIYLAVVAAFFVGLGCAFSEGAIFAQVPSMFPDNSGSVAGIVGGIGTVGGVVYPIAFSHAMLPSLHWGYTVVGASMIPIIALSAWVFQPHISEVANTSGWVVPDDLMSKTKGSPSDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYF9</accession>
    <name>A0A1U7EYF9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">CBS domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cbs6</name>
        <name evidence="4" type="ordered locus">NP_4330A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323872.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYF9"/>
    <dbReference type="STRING" id="348780.NP_4330A"/>
    <dbReference type="EnsemblBacteria" id="CAI50256">
        <property type="protein sequence ID" value="CAI50256"/>
        <property type="gene ID" value="NP_4330A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702584"/>
    <dbReference type="KEGG" id="nph:NP_4330A"/>
    <dbReference type="eggNOG" id="arCOG00606">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040681_12_2_2"/>
    <dbReference type="OrthoDB" id="8919at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09836">
        <property type="entry name" value="CBS_pair_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="12"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="74"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="144" mass="15328" checksum="9EE00975ECBAED45" modified="2017-05-10" version="1">MAEHDTNVESLMTSPVETIAPDASLADAATRLTEQSIGSLVVGEGQLRGIITESDIVTAVSEELDPETPVTELMSDPVVTIRRTETLQAAAERMGHNGVKKLPVVEGGSAIGIITTTDLALHLPNYQVNMAHQAEPDIVDGEWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYH5</accession>
    <name>A0A1U7EYH5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 19">Glutamine synthetase</fullName>
            <ecNumber evidence="3 19">6.3.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">glnA2</name>
        <name evidence="22" type="ordered locus">NP_4376A</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 19">
            <text>L-glutamate + NH4(+) + ATP = L-glutamine + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16169"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="14">Binds 2 Mg(2+) ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16 17">Belongs to the glutamine synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.2" evidence="3 19"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323895.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYH5"/>
    <dbReference type="STRING" id="348780.NP_4376A"/>
    <dbReference type="EnsemblBacteria" id="CAI50279">
        <property type="protein sequence ID" value="CAI50279"/>
        <property type="gene ID" value="NP_4376A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702755"/>
    <dbReference type="KEGG" id="nph:NP_4376A"/>
    <dbReference type="eggNOG" id="arCOG01909">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017290_1_3_2"/>
    <dbReference type="OrthoDB" id="36124at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamine synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.70:FF:000005">
        <property type="entry name" value="Glutamine synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000003">
        <property type="entry name" value="Glutamine synthetase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008147">
        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027303">
        <property type="entry name" value="Gln_synth_gly_rich_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004809">
        <property type="entry name" value="Gln_synth_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027302">
        <property type="entry name" value="Gln_synth_N_conserv_site"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00653">
        <property type="entry name" value="GlnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43785">
        <property type="entry name" value="GAMMA-GLUTAMYLPUTRESCINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43785:SF12">
        <property type="entry name" value="TYPE-1 GLUTAMINE SYNTHETASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03951">
        <property type="entry name" value="Gln-synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54368">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="GS_CATALYTIC"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 18" id="KW-0963">Cytoplasm</keyword>
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    <keyword evidence="8 13 19" id="KW-0547">Nucleotide-binding</keyword>
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        <location>
            <begin position="20"/>
            <end position="106"/>
        </location>
    </feature>
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        <location>
            <begin position="114"/>
            <end position="451"/>
        </location>
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        <location>
            <position position="137"/>
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        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
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        </ligand>
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        <location>
            <begin position="245"/>
            <end position="246"/>
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        <ligand>
            <name>L-glutamate</name>
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        </ligand>
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        <location>
            <position position="250"/>
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            <label>1</label>
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            <end position="254"/>
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        </ligand>
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            <name>L-glutamate</name>
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        </ligand>
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        </ligand>
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        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012937"/>
        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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        </source>
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        </source>
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        </source>
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        </source>
    </evidence>
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        </source>
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        </source>
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        </source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
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        <source>
            <dbReference type="RuleBase" id="RU000385"/>
        </source>
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        <source>
            <dbReference type="RuleBase" id="RU004356"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51986"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51987"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI50279.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="451" mass="50349" checksum="3D4272967971E2D0" modified="2017-05-10" version="1">MTDESLTPDEKRVLDEIEEKNVDFLRLQFIDILGTIKNVAVPADQAEKAFTEGIYFDGSSIEGFVRIQESDMRLKPDPSTFAILPWRKREDGASARMICDIYNTSTGEPFEGDPRYVLKQALERAEEMGYTVNAGPEPEFFLFEEDDEGRATTEFSDVGGYFDLAPKDLASDVRYDIIAGLESMGFDVEASHHEVAESQHEIDFEYDDALTTADNVATFRAVVRAIAAQHDLHATFMPKPIPKINGSGMHTHLSLFTEDGENAFHDGDDEFDLSDTARSFLAGVLEHAPAITAVANPTVNSYKRLVPGYEAPVYVAWSDTNRSALVRKPAARVPAASRIELRSPDPSCNPYLAFAAMIHAGLDGIENDLDCPDPVRENIYEFDEAKRDEYGIETLPSNLGEAVDALEADEVVLDALGEHVPEKFMEAKREEFGEYLVEVSEWELDRYLETF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYI6</accession>
    <name>A0A1U7EYI6_NATPD</name>
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        <submittedName>
            <fullName evidence="2">Thioredoxin domain protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">NP_4382A</name>
    </gene>
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        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
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        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
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            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
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    <dbReference type="KEGG" id="nph:NP_4382A"/>
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    <dbReference type="OrthoDB" id="146841at2157"/>
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        <property type="component" value="Chromosome"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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            <begin position="20"/>
            <end position="151"/>
        </location>
    </feature>
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        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI50282.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="156" mass="17451" checksum="605B823A53B3EECE" modified="2017-05-10" version="1">MNPRKVITIAVLVAVLGFGYYSMNAAPVLSDHDYTYQGGLQWHEDPDEALSIAQDEDKPVLVYYWTTWCTYCQTYDENHYQHDEIRDTLDAYTLLAINLDDPGPGASMAADHGASYPPQHVMMTPDGEEISQLGGFVERDQFLQEIESALGAVSNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYJ1</accession>
    <name>A0A1U7EYJ1_NATPD</name>
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        <submittedName>
            <fullName evidence="2">Sulfate permease family protein</fullName>
        </submittedName>
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        <name evidence="2" type="ordered locus">NP_4420A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
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        <lineage>
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            <taxon>Halobacteriales</taxon>
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            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
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                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAI50301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="NP_4420A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_4420A"/>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032158_1_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015098">
        <property type="term" value="F:molybdate ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031563">
        <property type="entry name" value="MOT1/MOT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31970:SF9">
        <property type="entry name" value="MOLYBDATE TRANSPORTER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16983">
        <property type="entry name" value="MFS_MOT1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="257"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="296"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="337"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="366" mass="36144" checksum="37CFDD0F46C12458" modified="2017-05-10" version="1">MYRLLALFVPPEAAFRTGRGFSFDAGEVTGAIGDSVTVLPLVVALGLLTPASLPHLLAGFGVFQVIWGLYYGVPLSVEPMKALAGLAIAGTLSHGGLVAAGLVAGGVLLVAGRTGSLSWIQQFVGEPVVRGVQFAVACLLVVAAVDLVAGSVGIAAAGAGLALVVALVSRNAVALVVLAVGAVWAATAGGIPAPAAPSLELFPAGGPTLSVGAIEGLLAQLAMTVGNAAVATSLLLSDLYDAEISPDRLADSMGAMNLAAVPLGAVPMCHGSGGLAGKHAFGARTGTANLVAGGFYAGLALVAGLLAAFPMALLGVLLVVVAASLSRVAFTKTDRRLFVAGVGGLAVVTNVAVAFVAATLWWQLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYM1</accession>
    <name>A0A1U7EYM1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Probable oxidoreductase (Aldo-keto reductase family protein)</fullName>
            <ecNumber evidence="3">1.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_4488A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323950.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYM1"/>
    <dbReference type="STRING" id="348780.NP_4488A"/>
    <dbReference type="EnsemblBacteria" id="CAI50335">
        <property type="protein sequence ID" value="CAI50335"/>
        <property type="gene ID" value="NP_4488A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702078"/>
    <dbReference type="KEGG" id="nph:NP_4488A"/>
    <dbReference type="eggNOG" id="arCOG01617">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_3_2"/>
    <dbReference type="OrthoDB" id="28487at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19086">
        <property type="entry name" value="AKR_AKR11C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053135">
        <property type="entry name" value="AKR2_Oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43312">
        <property type="entry name" value="D-THREO-ALDOSE 1-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43312:SF1">
        <property type="entry name" value="NADP-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="2">
        <location>
            <begin position="15"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="323"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="352" mass="39414" checksum="249722407AC82C79" modified="2017-05-10" version="1">MNYRTLGESGLEVSEIGFGAWVVGSDWWGDRTEQQAIDMVRYAVDQGITYFDTGDVYGHGDSEQLLGKALEPVRDEVTVATKIGYDFYNNPQAGHGELPKEMHRDYLEEAFEKSLDRLGMDSVEMLQLHNANADEIDADVLEFLDELREEGRVDAIGMALGPSIGWLAEGDLAIEEEFDAVQLVWNMLEQEVGNHFLDTLERTGSATSLIPRVPHSSGLLNEQVTPETELDEGDHRAYRPDEWYETGWEKIESLRFLERDGEREMSQAALQWLLFHDSVATVTPTFRTRDDIDEWAAASDVPPLSDEEYERVQSLYADNFGIEGDDGMDALRSSVGGEDIESAGLDKESAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYP8</accession>
    <name>A0A1U7EYP8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">GNAT family acetyltransferase</fullName>
            <ecNumber evidence="4">2.3.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_4540A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323976.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYP8"/>
    <dbReference type="STRING" id="348780.NP_4540A"/>
    <dbReference type="EnsemblBacteria" id="CAI50361">
        <property type="protein sequence ID" value="CAI50361"/>
        <property type="gene ID" value="NP_4540A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702093"/>
    <dbReference type="KEGG" id="nph:NP_4540A"/>
    <dbReference type="eggNOG" id="arCOG00837">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_23_1_2"/>
    <dbReference type="OrthoDB" id="87545at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050832">
        <property type="entry name" value="Bact_Acetyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43877:SF1">
        <property type="entry name" value="ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43877">
        <property type="entry name" value="AMINOALKYLPHOSPHONATE N-ACETYLTRANSFERASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="135" mass="14353" checksum="BF3B976864F795ED" modified="2017-05-10" version="1">MIRPLTEADRPAIETLQEHLTYADPSLVEAAVDGPFVGRLAVDEGMPAGYAIAFPGTTATLSELVVGPSFRRRGCASALVDAVDTAIAADGIAAVTPVENEAARSLYLDLGFEPDERLPEFYADGTDGLRFLRVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYS5</accession>
    <name>A0A1U7EYS5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Probable secreted glycoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">NP_4620A</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Cell wall</location>
            <location evidence="2">S-layer</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the halobacterial S-layer protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324016.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYS5"/>
    <dbReference type="EnsemblBacteria" id="CAI50401">
        <property type="protein sequence ID" value="CAI50401"/>
        <property type="gene ID" value="NP_4620A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702120"/>
    <dbReference type="KEGG" id="nph:NP_4620A"/>
    <dbReference type="eggNOG" id="arCOG06273">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006819_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030115">
        <property type="term" value="C:S-layer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057149">
        <property type="entry name" value="DUF7827"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026371">
        <property type="entry name" value="PGF_CTERM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026452">
        <property type="entry name" value="Surf_glycop_sig_pep"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04207">
        <property type="entry name" value="halo_sig_pep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045517">
        <property type="entry name" value="halo_surf_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04126">
        <property type="entry name" value="PGF_CTERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25162">
        <property type="entry name" value="DUF7827"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18204">
        <property type="entry name" value="PGF-CTERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0134">Cell wall</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0701">S-layer</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="8 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1017"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7827" evidence="16">
        <location>
            <begin position="465"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="PGF-CTERM archaeal protein-sorting signal" evidence="15">
        <location>
            <begin position="1018"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="964"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="971"/>
            <end position="1015"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF18204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF25162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAI50401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="1044" mass="114099" checksum="899E625704F0E9E2" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYW0</accession>
    <name>A0A1U7EYW0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GalE family epimerase/dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4662A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324036.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYW0"/>
    <dbReference type="STRING" id="348780.NP_4662A"/>
    <dbReference type="EnsemblBacteria" id="CAI50422">
        <property type="protein sequence ID" value="CAI50422"/>
        <property type="gene ID" value="NP_4662A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702734"/>
    <dbReference type="KEGG" id="nph:NP_4662A"/>
    <dbReference type="eggNOG" id="arCOG01369">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_7_2"/>
    <dbReference type="OrthoDB" id="4907at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43000">
        <property type="entry name" value="DTDP-D-GLUCOSE 4,6-DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01713">
        <property type="entry name" value="NUCEPIMERASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="1">
        <location>
            <begin position="4"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="328" mass="36003" checksum="9AE8D13D8E6D7517" modified="2017-05-10" version="1">MQVLVTGGAGFIGGHLAEQFAADGHDVVVLDNFEPYYDLGIKEHNVEAARDAAKANGATYKLIDGSITDDDQVDTLVSEADVIYHQAAQAGVRKSVEQPAKVNAYNVDGTVTLLEAARRHDVERVVLASSSSVYGKPEYLPYDEAHPTTPVSPYGVSKLAAEQYARVYSEVYGLPTVGLRYFTVYGPRMRPNMAMTNFVSRCLHGESPVIYGDGTQTRDFTYVADIKRVNAQLLNDDSADGEILNIGSTDTIDIQTLAEVVRDEIDPSLDIEYTDPREGDAEHTHADISKANALLGYEPTVDIREGVSAFIDWYRENKAWYDPLVRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ24</accession>
    <name>A0A1U7EZ24_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">isopentenyl-diphosphate Delta-isomerase</fullName>
            <ecNumber evidence="3">5.3.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">idiA</name>
        <name evidence="7" type="ordered locus">NP_4826A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from isopentenyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IPP isomerase type 1 family.</text>
    </comment>
    <dbReference type="EC" id="5.3.3.2" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50504.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324116.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ24"/>
    <dbReference type="STRING" id="348780.NP_4826A"/>
    <dbReference type="EnsemblBacteria" id="CAI50504">
        <property type="protein sequence ID" value="CAI50504"/>
        <property type="gene ID" value="NP_4826A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702857"/>
    <dbReference type="KEGG" id="nph:NP_4826A"/>
    <dbReference type="eggNOG" id="arCOG01081">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060552_2_1_2"/>
    <dbReference type="OrthoDB" id="4234at2157"/>
    <dbReference type="UniPathway" id="UPA00059">
        <property type="reaction ID" value="UER00104"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004452">
        <property type="term" value="F:isopentenyl-diphosphate delta-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050992">
        <property type="term" value="P:dimethylallyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009240">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02885">
        <property type="entry name" value="NUDIX_IPP_Isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011876">
        <property type="entry name" value="IsopentenylPP_isomerase_typ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10885">
        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10885:SF0">
        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018427">
        <property type="entry name" value="Isopntndiph_ism"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="6">
        <location>
            <begin position="42"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI50504.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="188" mass="21537" checksum="CD24B293CC9E4383" modified="2017-05-10" version="1">MDTTDAETADHPNAGQDVIAVDSDDNAAGTVNRLDAHTGDGIRHRAFTVLVFDGDGNVLLAQRAPTKRLWDTHWDGTVASHPVEGQTQLEATRERLEEELGVTPDQYSDLRVTDKFEYKRYYLDAGLEWEVCSVLQCTLDDVSLDPDEEEVAGVLWAPYERLYENQRWYRQLRLCPWFEIAMRRDFEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ45</accession>
    <name>A0A1U7EZ45_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4942A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324174.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ45"/>
    <dbReference type="STRING" id="348780.NP_4942A"/>
    <dbReference type="EnsemblBacteria" id="CAI50562">
        <property type="protein sequence ID" value="CAI50562"/>
        <property type="gene ID" value="NP_4942A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702207"/>
    <dbReference type="KEGG" id="nph:NP_4942A"/>
    <dbReference type="eggNOG" id="arCOG10320">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2712948_0_0_2"/>
    <dbReference type="OrthoDB" id="197586at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="72" mass="8074" checksum="48DC5620590988B7" modified="2017-05-10" version="1">MTWEFIYPGTWVIFAVIGLPVYTVVLAWFLGKPRTIKTSLMGLSYLIGIIVSMWVGLYILTQLLGIVLPPAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ51</accession>
    <name>A0A1U7EZ51_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4972A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324188.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ51"/>
    <dbReference type="STRING" id="348780.NP_4972A"/>
    <dbReference type="EnsemblBacteria" id="CAI50577">
        <property type="protein sequence ID" value="CAI50577"/>
        <property type="gene ID" value="NP_4972A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702217"/>
    <dbReference type="KEGG" id="nph:NP_4972A"/>
    <dbReference type="eggNOG" id="arCOG06264">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123157_0_0_2"/>
    <dbReference type="OrthoDB" id="193446at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="132" mass="14638" checksum="3FFE2CBC68AF87FF" modified="2017-05-10" version="1">MRMELRVCKHCYTGEHGNEQKTAITQDMVACAEQIREYKDLISLESVYITKVEEGGSDRGEAIPVVVAGIEGDTVTLRDTQLVIEDDDESVLVYPEPADILDVLTRNLDQINQRTRQDVSVALSETGAELVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ81</accession>
    <name>A0A1U7EZ81_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC-type transport system permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">abc03p</name>
        <name evidence="10" type="ordered locus">NP_5004A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324203.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ81"/>
    <dbReference type="STRING" id="348780.NP_5004A"/>
    <dbReference type="EnsemblBacteria" id="CAI50593">
        <property type="protein sequence ID" value="CAI50593"/>
        <property type="gene ID" value="NP_5004A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702371"/>
    <dbReference type="KEGG" id="nph:NP_5004A"/>
    <dbReference type="eggNOG" id="arCOG00163">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021838_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43357:SF3">
        <property type="entry name" value="FE(3+)-TRANSPORT SYSTEM PERMEASE PROTEIN FBPB 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43357">
        <property type="entry name" value="INNER MEMBRANE ABC TRANSPORTER PERMEASE PROTEIN YDCV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="23"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="69"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="105"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="294"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="338"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="373"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="454"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="504"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="70"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="335"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI50593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="535" mass="57905" checksum="AFCB5DF9CD7D862C" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZC6</accession>
    <name>A0A1U7EZC6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ParA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">parA4</name>
        <name evidence="5" type="ordered locus">NP_5092A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324247.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZC6"/>
    <dbReference type="STRING" id="348780.NP_5092A"/>
    <dbReference type="EnsemblBacteria" id="CAI50637">
        <property type="protein sequence ID" value="CAI50637"/>
        <property type="gene ID" value="NP_5092A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702251"/>
    <dbReference type="KEGG" id="nph:NP_5092A"/>
    <dbReference type="eggNOG" id="arCOG07866">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1122604_0_0_2"/>
    <dbReference type="OrthoDB" id="313523at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051782">
        <property type="term" value="P:negative regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025669">
        <property type="entry name" value="AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050625">
        <property type="entry name" value="ParA/MinD_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384:SF6">
        <property type="entry name" value="SEPTUM SITE-DETERMINING PROTEIN MIND HOMOLOG, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384">
        <property type="entry name" value="SEPTUM SITE-DETERMINING PROTEIN MIND HOMOLOG, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13614">
        <property type="entry name" value="AAA_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="4">
        <location>
            <begin position="7"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI50637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="235" mass="24263" checksum="2C879F7A6FBE8706" modified="2017-05-10" version="1">MNGTTAAFVGATGGAGTTRLTLECGHLLASAGHDTAVFDAAYATQGLADHVSGRIDTDVTALCLGDERLEAALYDQQLQGAGRLAVCPARAPFERFARAKRPEAAEAFEDQVDAAARQFDYVLVDTPPVAGNQAVAAVNAAEAVAVVCDADRAADAVPRMRDRLADVGAEPDATVVTHATAHEEGDVTVPPFEADKPGSDEDAPGYPALVDVVNTVTEASVPEPEAEGVLDRLVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZC7</accession>
    <name>A0A1U7EZC7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Acetate--CoA ligase</fullName>
            <ecNumber evidence="2 6">6.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">acs3</name>
        <name evidence="10" type="ordered locus">NP_5128A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.1" evidence="2 6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324265.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZC7"/>
    <dbReference type="STRING" id="348780.NP_5128A"/>
    <dbReference type="EnsemblBacteria" id="CAI50655">
        <property type="protein sequence ID" value="CAI50655"/>
        <property type="gene ID" value="NP_5128A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702486"/>
    <dbReference type="KEGG" id="nph:NP_5128A"/>
    <dbReference type="eggNOG" id="arCOG04201">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_3_6_2"/>
    <dbReference type="OrthoDB" id="371752at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043955">
        <property type="term" value="F:3-hydroxypropionyl-CoA synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019427">
        <property type="term" value="P:acetyl-CoA biosynthetic process from acetate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043427">
        <property type="term" value="P:carbon fixation by 3-hydroxypropionate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000001">
        <property type="entry name" value="Acetyl-coenzyme A synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011904">
        <property type="entry name" value="Ac_CoA_lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02188">
        <property type="entry name" value="Ac_CoA_lig_AcsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001208">
        <property type="entry name" value="PRK00174.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095:SF14">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="9">
        <location>
            <begin position="35"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="7">
        <location>
            <begin position="95"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="8">
        <location>
            <begin position="543"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="NCBIfam" id="TIGR02188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI50655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="662" mass="73368" checksum="15E3816AE3E65BB2" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZE9</accession>
    <name>A0A1U7EZE9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Probable S-adenosylmethionine-dependent methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_5170A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324286.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZE9"/>
    <dbReference type="STRING" id="348780.NP_5170A"/>
    <dbReference type="EnsemblBacteria" id="CAI50676">
        <property type="protein sequence ID" value="CAI50676"/>
        <property type="gene ID" value="NP_5170A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702280"/>
    <dbReference type="KEGG" id="nph:NP_5170A"/>
    <dbReference type="eggNOG" id="arCOG02703">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080641_0_0_2"/>
    <dbReference type="OrthoDB" id="182741at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43861:SF1">
        <property type="entry name" value="TRANS-ACONITATE 2-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43861">
        <property type="entry name" value="TRANS-ACONITATE 2-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="3">
        <location>
            <begin position="42"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="220" mass="23715" checksum="EED3D4A151CECE83" modified="2017-05-10" version="1">MASVEAFYGRWADLYDHIATLPGVARWRQRAAERVADRGATIVEMGCGTGANLPFLREQVGPEGRVVGIDITRPLLDRARTRASEYDNVAVLHGDAVVPPVASADGVLATFVCGLFENPERVVDQWCDRLNDGGRVALLDATPSEQPLGVPLNPLFSMFVAAGSPTTDARDVLRAPCSGHGTELERRVAASRRALAARARDRRYETFGLGFVGLASGRID</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZF7</accession>
    <name>A0A1U7EZF7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidyl-tRNA hydrolase</fullName>
            <ecNumber evidence="2">3.1.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pth</name>
        <name evidence="7" type="ordered locus">NP_5224A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>an N-acyl-L-alpha-aminoacyl-tRNA + H2O = an N-acyl-L-amino acid + a tRNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:13883"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59874"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:138191"/>
            <dbReference type="EC" id="3.1.1.29"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PTH2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.29" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50703.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939755.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZF7"/>
    <dbReference type="STRING" id="348780.NP_5224A"/>
    <dbReference type="EnsemblBacteria" id="CAI50703">
        <property type="protein sequence ID" value="CAI50703"/>
        <property type="gene ID" value="NP_5224A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702302"/>
    <dbReference type="KEGG" id="nph:NP_5224A"/>
    <dbReference type="eggNOG" id="arCOG04228">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073661_2_2_2"/>
    <dbReference type="OrthoDB" id="6075at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:peptidyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02430">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1490.10:FF:000001">
        <property type="entry name" value="Peptidyl-tRNA hydrolase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1490.10">
        <property type="entry name" value="Bit1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023476">
        <property type="entry name" value="Pep_tRNA_hydro_II_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002833">
        <property type="entry name" value="PTH2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00283">
        <property type="entry name" value="arch_pth2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003314">
        <property type="entry name" value="PRK04322.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649:SF11">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01981">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102462">
        <property type="entry name" value="Peptidyl-tRNA hydrolase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI50703.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="112" mass="11934" checksum="2AEC275861D7D6B3" modified="2017-05-10" version="1">MKQAIVARTDIDMGTGKLAAQVAHASLSAYEDAGDEARRAWKSGGQKKIVLKGSGESQLQQLADEARRERLPHALVRDAGHTQLEPGTVTALAVGPGEERLVDKVTGDLSLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZG8</accession>
    <name>A0A1U7EZG8_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HTH domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_5216A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324309.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZG8"/>
    <dbReference type="STRING" id="348780.NP_5216A"/>
    <dbReference type="EnsemblBacteria" id="CAI50699">
        <property type="protein sequence ID" value="CAI50699"/>
        <property type="gene ID" value="NP_5216A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702299"/>
    <dbReference type="KEGG" id="nph:NP_5216A"/>
    <dbReference type="eggNOG" id="arCOG04674">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123164_0_0_2"/>
    <dbReference type="OrthoDB" id="306692at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043809">
        <property type="entry name" value="DUF5791"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19104">
        <property type="entry name" value="DUF5791"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="141" mass="15046" checksum="964F2A322C5E7E8D" modified="2017-05-10" version="1">MFHELTDDPGGTSPAAIEAAYVSALEAVLDEHDIGTAAAETGIERSALSALLDGELPELTLEDAAELLALREDAPDAGTIVALSRDALLMGMTTAVLDVEAVESEIGGELEAREIQSKVEGRYPMTLSEFALLFHHIERRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZJ5</accession>
    <name>A0A1U7EZJ5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">FtsZ family protein, noncanonical</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ftsZ5</name>
        <name evidence="6" type="ordered locus">NP_5294A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZJ5"/>
    <dbReference type="STRING" id="348780.NP_5294A"/>
    <dbReference type="EnsemblBacteria" id="CAI50738">
        <property type="protein sequence ID" value="CAI50738"/>
        <property type="gene ID" value="NP_5294A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_5294A"/>
    <dbReference type="eggNOG" id="arCOG02201">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_870493_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045061">
        <property type="entry name" value="FtsZ/CetZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024757">
        <property type="entry name" value="FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314">
        <property type="entry name" value="CELL DIVISION PROTEIN FTSZ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314:SF3">
        <property type="entry name" value="MITOCHONDRIAL DIVISION PROTEIN FSZA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12327">
        <property type="entry name" value="FtsZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00423">
        <property type="entry name" value="CELLDVISFTSZ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0717">Septation</keyword>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="5">
        <location>
            <begin position="162"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI50738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="319" mass="32386" checksum="7FBE1466609039FB" modified="2017-05-10" version="1">MIDAAVAARQAGNTPHCWWYRKRSYGGRSNRRQVTTDEELLAAADALREAVLVAGNGSTGTAVADWVAAGDAPAATDVETPDVSVVAVDAADADASADIESPGALLSVAVVAVPQRPSAGERRLLTALESTVDTVIVTTGDAPELTTAVEAFVSMVRDAGFVNVDLADARTVFEPVAQAALGIGESPGGTPGEAVEEAIAALPPGVETDTASGVLVDLRGGPSMSVGDVNDAVTAVREQVGTEAHVIWGGKVDEALSETVVVRLIAAGVDNVRAAPGDDCPRCDRGLFAYTLSGLPGLSCDTCGFGGVSTGRKKNSESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZK6</accession>
    <name>A0A1U7EZK6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5314A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324357.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZK6"/>
    <dbReference type="STRING" id="348780.NP_5314A"/>
    <dbReference type="EnsemblBacteria" id="CAI50748">
        <property type="protein sequence ID" value="CAI50748"/>
        <property type="gene ID" value="NP_5314A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702329"/>
    <dbReference type="KEGG" id="nph:NP_5314A"/>
    <dbReference type="eggNOG" id="arCOG15170">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2949406_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="59" mass="5885" checksum="8395FC50D2C15DB1" modified="2017-05-10" version="1">MVDKLGLAGMLGFGLFIGAFFGYVLSWGDIVMAGAGAVGLAVIALIYTFTLGQSGAPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILS1</accession>
    <name>Q3ILS1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3244A</name>
        <name evidence="2" type="ordered locus">NP_7068A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>PL23</name>
    </geneLocation>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
            <strain evidence="2">Gabara</strain>
            <plasmid evidence="2">PL23</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CR936259">
        <property type="protein sequence ID" value="CAI50950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323335.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILS1"/>
    <dbReference type="STRING" id="348780.NP_3244A"/>
    <dbReference type="EnsemblBacteria" id="CAI49713">
        <property type="protein sequence ID" value="CAI49713"/>
        <property type="gene ID" value="NP_3244A"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI50950">
        <property type="protein sequence ID" value="CAI50950"/>
        <property type="gene ID" value="NP_7068A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703493"/>
    <dbReference type="KEGG" id="nph:NP_3244A"/>
    <dbReference type="KEGG" id="nph:NP_7068A"/>
    <dbReference type="eggNOG" id="arCOG06155">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2662458_0_0_2"/>
    <dbReference type="OrthoDB" id="189961at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="79" mass="8746" checksum="F552DEEB3366E9D7" modified="2005-11-08" version="1">MTTGYVVNLDNGHNGFCVESLLGVTHCGETFDRDAVELETFTIDGRDGDHPHSQLCYECSKVDSVALRDSVRRQEVVAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILT7</accession>
    <name>Q3ILT7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3310A</name>
        <name evidence="2" type="ordered locus">NP_7034A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>PL23</name>
    </geneLocation>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
            <strain evidence="2">Gabara</strain>
            <plasmid evidence="2">PL23</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CR936259">
        <property type="protein sequence ID" value="CAI50933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323367.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILT7"/>
    <dbReference type="STRING" id="348780.NP_3310A"/>
    <dbReference type="EnsemblBacteria" id="CAI49746">
        <property type="protein sequence ID" value="CAI49746"/>
        <property type="gene ID" value="NP_3310A"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI50933">
        <property type="protein sequence ID" value="CAI50933"/>
        <property type="gene ID" value="NP_7034A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703477"/>
    <dbReference type="KEGG" id="nph:NP_3310A"/>
    <dbReference type="KEGG" id="nph:NP_7034A"/>
    <dbReference type="HOGENOM" id="CLU_1212629_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL23"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="228" mass="24382" checksum="22854C4F1CFD60E6" modified="2005-11-08" version="1">MTDRVEELESRVEQLEQIIESQSAGPSRRSVLASLAGVAGAGALGAGVGSQRAAAQEAVGQVGTEADPVDLFANSIDAESASIGEVAGLMYSTSDQPIPDNTSTQVEWNTVEIEDSDIVDADTGNDRFVIQEDGTYYVESSILWGTGLSWGEGDRAQLSIQGDVGLFINRTPAHVGWVGEEKHMKISRVYEISEGDSIEIFVEQRTGGEVELRHFSGMTNAFQVVKLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILT8</accession>
    <name>Q3ILT8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3308A</name>
        <name evidence="3" type="ordered locus">NP_7032A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>PL23</name>
    </geneLocation>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
            <strain evidence="3">Gabara</strain>
            <plasmid evidence="3">PL23</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CR936259">
        <property type="protein sequence ID" value="CAI50932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILT8"/>
    <dbReference type="EnsemblBacteria" id="CAI49745">
        <property type="protein sequence ID" value="CAI49745"/>
        <property type="gene ID" value="NP_3308A"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI50932">
        <property type="protein sequence ID" value="CAI50932"/>
        <property type="gene ID" value="NP_7032A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3308A"/>
    <dbReference type="KEGG" id="nph:NP_7032A"/>
    <dbReference type="HOGENOM" id="CLU_3338562_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="37" mass="4365" checksum="485DDE1A8AF98A45" modified="2005-11-08" version="1">MTDEELQRLRDEAVQEQRGRNAERPLAATRTEIKTHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILW0</accession>
    <name>Q3ILW0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transport protein (Probable substrate cationic amino acids)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cat4</name>
        <name evidence="9" type="synonym">tp03</name>
        <name evidence="9" type="ordered locus">NP_6260A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9 10">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324507.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILW0"/>
    <dbReference type="SMR" id="Q3ILW0"/>
    <dbReference type="EnsemblBacteria" id="CAI50910">
        <property type="protein sequence ID" value="CAI50910"/>
        <property type="gene ID" value="NP_6260A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694603"/>
    <dbReference type="KEGG" id="nph:NP_6260A"/>
    <dbReference type="HOGENOM" id="CLU_007946_15_8_2"/>
    <dbReference type="OrthoDB" id="56838at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050367">
        <property type="entry name" value="APC_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770">
        <property type="entry name" value="AMINO ACID TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770:SF11">
        <property type="entry name" value="INNER MEMBRANE TRANSPORT PROTEIN YBAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="84"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="188"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="222"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="264"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="354"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="385"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="413"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="UspA" evidence="8">
        <location>
            <begin position="456"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="domain" description="UspA" evidence="8">
        <location>
            <begin position="608"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="724"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="728"/>
            <end position="748"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI50910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="748" mass="78497" checksum="D44F41F1D64B90F9" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM33</accession>
    <name>Q3IM33_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC-type transport system periplasmic substrate-binding protein (Probable substrate phosphate)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">pstS2</name>
        <name evidence="3" type="synonym">abc15s</name>
        <name evidence="3" type="ordered locus">NP_6104A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324434.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM33"/>
    <dbReference type="EnsemblBacteria" id="CAI50832">
        <property type="protein sequence ID" value="CAI50832"/>
        <property type="gene ID" value="NP_6104A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694674"/>
    <dbReference type="KEGG" id="nph:NP_6104A"/>
    <dbReference type="HOGENOM" id="CLU_026228_1_0_2"/>
    <dbReference type="OrthoDB" id="53390at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="CDD" id="cd13654">
        <property type="entry name" value="PBP2_phosphate_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024370">
        <property type="entry name" value="PBP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050811">
        <property type="entry name" value="Phosphate_ABC_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30570">
        <property type="entry name" value="PERIPLASMIC PHOSPHATE BINDING COMPONENT OF PHOSPHATE ABC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30570:SF1">
        <property type="entry name" value="PHOSPHATE-BINDING PROTEIN PSTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12849">
        <property type="entry name" value="PBP_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="PBP" evidence="2">
        <location>
            <begin position="38"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="337" mass="36697" checksum="A723B004E70FCFBD" modified="2005-11-08" version="1">MAHTPSDRVSRRSVLATTGAAAAFGLAGCLGGGGGDGLSGTIDASGSNTVAPITSWAGENFSNEFPDVLVDVDPQGTGAGFQEFCRQNSDVQSASRLITDEEVDLCNENDVNYDHLEVGLDGLAVVKNSENDWVDNITLDELQQVWEFQSDVETWSDIRSEWPDREIALHARDSASGTFDYFTRAINGEIGNIRDDYSATSQTNEIMGAVADNIDGFGWGGVGYLRAIEDDQPIEAVPVESDSVDGFFLPQRENVESGDYSPLARPLFAYFNLASLEERTDLIGSFARFYTNDAQDYARDEGFFAAPPEVTDENHDKIDSWLDQVGASPDDLTVQRE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-11-03" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMN8</accession>
    <name>DABA_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable inorganic carbon transporter subunit DabA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dabA</name>
        <name type="ordered locus">NP_5058A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of an energy-coupled inorganic carbon pump.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a complex with DabB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the inorganic carbon transporter (TC 9.A.2) DabA family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50620.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324230.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMN8"/>
    <dbReference type="STRING" id="348780.NP_5058A"/>
    <dbReference type="EnsemblBacteria" id="CAI50620">
        <property type="protein sequence ID" value="CAI50620"/>
        <property type="gene ID" value="NP_5058A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702242"/>
    <dbReference type="KEGG" id="nph:NP_5058A"/>
    <dbReference type="eggNOG" id="arCOG04520">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009885_0_0_2"/>
    <dbReference type="OrthoDB" id="185928at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01871">
        <property type="entry name" value="DabA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018752">
        <property type="entry name" value="DabA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38344:SF1">
        <property type="entry name" value="INORGANIC CARBON TRANSPORTER SUBUNIT DABA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38344">
        <property type="entry name" value="UPF0753 PROTEIN AQ_863"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10070">
        <property type="entry name" value="DabA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000387332" description="Probable inorganic carbon transporter subunit DabA">
        <location>
            <begin position="1"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="574"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="479"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="494"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="799" mass="87771" checksum="73A7C57EE2190194" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-07-24" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q3INP4</accession>
    <name>PANB_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-methyl-2-oxobutanoate hydroxymethyltransferase</fullName>
            <ecNumber evidence="1">2.1.2.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Ketopantoate hydroxymethyltransferase</fullName>
            <shortName evidence="1">KPHMT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">panB</name>
        <name type="ordered locus">NP_4334A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is transferred onto alpha-ketoisovalerate to form ketopantoate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = 2-dehydropantoate + (6S)-5,6,7,8-tetrahydrofolate</text>
            <dbReference type="Rhea" id="RHEA:11824"/>
            <dbReference type="ChEBI" id="CHEBI:11561"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodecamer; pentamer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PanB family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.11" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323874.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3INP4"/>
    <dbReference type="SMR" id="Q3INP4"/>
    <dbReference type="STRING" id="348780.NP_4334A"/>
    <dbReference type="EnsemblBacteria" id="CAI50258">
        <property type="protein sequence ID" value="CAI50258"/>
        <property type="gene ID" value="NP_4334A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703006"/>
    <dbReference type="KEGG" id="nph:NP_4334A"/>
    <dbReference type="eggNOG" id="arCOG00584">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036645_1_0_2"/>
    <dbReference type="OrthoDB" id="8414at2157"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003864">
        <property type="term" value="F:3-methyl-2-oxobutanoate hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06557">
        <property type="entry name" value="KPHMT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.60:FF:000003">
        <property type="entry name" value="3-methyl-2-oxobutanoate hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00156">
        <property type="entry name" value="PanB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003700">
        <property type="entry name" value="Pantoate_hydroxy_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00222">
        <property type="entry name" value="panB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001452">
        <property type="entry name" value="PRK00311.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20881">
        <property type="entry name" value="3-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20881:SF0">
        <property type="entry name" value="3-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02548">
        <property type="entry name" value="Pantoate_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000388">
        <property type="entry name" value="Pantoate_hydroxy_MeTrfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000297423" description="3-methyl-2-oxobutanoate hydroxymethyltransferase">
        <location>
            <begin position="1"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="41"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>3-methyl-2-oxobutanoate</name>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>3-methyl-2-oxobutanoate</name>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>3-methyl-2-oxobutanoate</name>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00156"/>
        </source>
    </evidence>
    <sequence length="268" mass="28200" checksum="3C0E034F4CEC1693" modified="2005-11-08" version="1">MRAKDIRAKAGDEPITMLTAYDAPTAEIAEAAGVDVLLVGDSVGNTRLGYDSTLPVTVDEMASHTAAVARATDDALVVADMPFLSFGADEADAVDNCGRMLKEADADAVKLECGPHTVSLTKTLSDLGIPVQAHLGLTPQRENETGLYRQGTDEESANRILELAREHEAAGAFSLVLEHVPANLAASVTDALSIPTIGIGAGPDCDGQVLVVDEVVGLAEGTAPFSKRFGDVRGEMREAIEAYTDAVDSGSFPGDEHSHYEDDIEDIY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-03-03" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IP06</accession>
    <name>TRM56_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA (cytidine(56)-2'-O)-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.206</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA ribose 2'-O-methyltransferase aTrm56</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">NP_4110A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically catalyzes the AdoMet-dependent 2'-O-ribose methylation of cytidine at position 56 in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>cytidine(56) in tRNA + S-adenosyl-L-methionine = 2'-O-methylcytidine(56) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42968"/>
            <dbReference type="Rhea" id="RHEA-COMP:10308"/>
            <dbReference type="Rhea" id="RHEA-COMP:10309"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.206"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aTrm56 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.206" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323762.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IP06"/>
    <dbReference type="SMR" id="Q3IP06"/>
    <dbReference type="STRING" id="348780.NP_4110A"/>
    <dbReference type="EnsemblBacteria" id="CAI50146">
        <property type="protein sequence ID" value="CAI50146"/>
        <property type="gene ID" value="NP_4110A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701976"/>
    <dbReference type="KEGG" id="nph:NP_4110A"/>
    <dbReference type="eggNOG" id="arCOG01857">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123709_0_0_2"/>
    <dbReference type="OrthoDB" id="14397at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106059">
        <property type="term" value="F:tRNA (cytidine(56)-2'-O)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002128">
        <property type="term" value="P:tRNA nucleoside ribose methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18083">
        <property type="entry name" value="aTrm56-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00077">
        <property type="entry name" value="tRNA_methyltr_aTrm56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002845">
        <property type="entry name" value="tRNA_mtfrase_aTrm56"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003048">
        <property type="entry name" value="PRK03958.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42197">
        <property type="entry name" value="TRNA (CYTIDINE(56)-2'-O)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42197:SF1">
        <property type="entry name" value="TRNA (CYTIDINE(56)-2'-O)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01994">
        <property type="entry name" value="Trm56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016123">
        <property type="entry name" value="UCP016123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000365317" description="tRNA (cytidine(56)-2'-O)-methyltransferase">
        <location>
            <begin position="1"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="112"/>
            <end position="116"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00077"/>
        </source>
    </evidence>
    <sequence length="180" mass="19909" checksum="DEF85EA0CD7E7C58" modified="2005-11-08" version="1">MHGENEVVVLRLGHRPGRDNRMTTHVGLTARALGADRVSIAGAASDSKATIEDITDRFGGPFEVELTTEPRALVRDWAGTVVHLTMYGQRIQDVEADIREAHASGPVLIVVGAEKVPFDVYEQADYNVGVTNQPHSEVAGLAVFLDRLFEGRQLDREWENADRRVVPKETGKRVEPVDEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-04-08" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IPI5</accession>
    <name>ACYP_NATPD</name>
    <protein>
        <recommendedName>
            <fullName>Acylphosphatase</fullName>
            <ecNumber>3.6.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Acylphosphate phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">acyP</name>
        <name type="ordered locus">NP_3750A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>an acyl phosphate + H2O = a carboxylate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59918"/>
            <dbReference type="EC" id="3.6.1.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acylphosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323583.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IPI5"/>
    <dbReference type="SMR" id="Q3IPI5"/>
    <dbReference type="STRING" id="348780.NP_3750A"/>
    <dbReference type="EnsemblBacteria" id="CAI49966">
        <property type="protein sequence ID" value="CAI49966"/>
        <property type="gene ID" value="NP_3750A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702491"/>
    <dbReference type="KEGG" id="nph:NP_3750A"/>
    <dbReference type="eggNOG" id="arCOG01674">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141932_3_2_2"/>
    <dbReference type="OrthoDB" id="6643at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003998">
        <property type="term" value="F:acylphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020456">
        <property type="entry name" value="Acylphosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001792">
        <property type="entry name" value="Acylphosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036046">
        <property type="entry name" value="Acylphosphatase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017968">
        <property type="entry name" value="Acylphosphatase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011016">
        <property type="entry name" value="PRK14444.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47268">
        <property type="entry name" value="ACYLPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47268:SF4">
        <property type="entry name" value="ACYLPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00708">
        <property type="entry name" value="Acylphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00112">
        <property type="entry name" value="ACYLPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54975">
        <property type="entry name" value="Acylphosphatase/BLUF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00151">
        <property type="entry name" value="ACYLPHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51160">
        <property type="entry name" value="ACYLPHOSPHATASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000326863" description="Acylphosphatase">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Acylphosphatase-like" evidence="1">
        <location>
            <begin position="6"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="21"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="92" mass="10430" checksum="971056BAACED48C0" modified="2005-11-08" version="1">MSDRIRAHVYVSGRVQGVYYRANTRDTARERDIDGWVRNLDDGRVEAVFEGPREAVESMVGWCHTGSPKARVESVDAAYDDPEGVDGFEIRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IRM5</accession>
    <name>RNZ_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease Z</fullName>
            <shortName evidence="1">RNase Z</shortName>
            <ecNumber evidence="1">3.1.26.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA 3 endonuclease</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNase Z</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rnz</name>
        <name type="ordered locus">NP_2254A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.</text>
            <dbReference type="EC" id="3.1.26.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) ions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNase Z family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.11" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322845.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IRM5"/>
    <dbReference type="SMR" id="Q3IRM5"/>
    <dbReference type="STRING" id="348780.NP_2254A"/>
    <dbReference type="EnsemblBacteria" id="CAI49218">
        <property type="protein sequence ID" value="CAI49218"/>
        <property type="gene ID" value="NP_2254A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701393"/>
    <dbReference type="KEGG" id="nph:NP_2254A"/>
    <dbReference type="eggNOG" id="arCOG00501">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031317_2_1_2"/>
    <dbReference type="OrthoDB" id="85118at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042781">
        <property type="term" value="F:3'-tRNA processing endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07717">
        <property type="entry name" value="RNaseZ_ZiPD-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000002">
        <property type="entry name" value="Ribonuclease Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01818">
        <property type="entry name" value="RNase_Z_BN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013471">
        <property type="entry name" value="RNase_Z/BN"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000801">
        <property type="entry name" value="PRK00055.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02651">
        <property type="entry name" value="RNase_Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46018">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46018:SF2">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23023">
        <property type="entry name" value="Anti-Pycsar_Apyc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_1000070309" description="Ribonuclease Z">
        <location>
            <begin position="1"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01818"/>
        </source>
    </evidence>
    <sequence length="308" mass="33420" checksum="149C50942AAC5BB8" modified="2005-11-08" version="1">MSLRVTFLGTSGAVPTTRRNTSAVFLRREGDRMLFDCGEGTQRQMMRFGTGFAVSKAFITHLHGDHILGLPGLLQTMDFNDREEPLSVYVPSGKQAELRDLIDTAAGAPSFPVHVNGVSDGQVVVDADDYEIRAFETDHDARSLGYALIEASRKGRFDRERAEELGVPVGPKFQQLHAGEAVELEDGTVVEPEQVVGDPRPGRRVVYTGDTRPTDRTVTVAENADLLIHDATFATDNADRAARTAHSTAAEAADIASRADARRLALVHISSRYAGDPSPIEREADDAFGGEAFVPEDGDTVDIPFPDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IS11</accession>
    <name>Q3IS11_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PhoU domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">phoU1</name>
        <name evidence="2" type="synonym">abc14r1</name>
        <name evidence="2" type="ordered locus">NP_1976A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322709.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IS11"/>
    <dbReference type="STRING" id="348780.NP_1976A"/>
    <dbReference type="EnsemblBacteria" id="CAI49079">
        <property type="protein sequence ID" value="CAI49079"/>
        <property type="gene ID" value="NP_1976A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702414"/>
    <dbReference type="KEGG" id="nph:NP_1976A"/>
    <dbReference type="eggNOG" id="arCOG00318">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069302_0_0_2"/>
    <dbReference type="OrthoDB" id="40991at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:intracellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045936">
        <property type="term" value="P:negative regulation of phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="entry name" value="Phosphate transport system protein phou homolog 2, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028366">
        <property type="entry name" value="PhoU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026022">
        <property type="entry name" value="PhoU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930:SF6">
        <property type="entry name" value="PHOSPHATE REGULATORY PROTEIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930">
        <property type="entry name" value="PHOSPHATE-SPECIFIC TRANSPORT SYSTEM ACCESSORY PROTEIN PHOU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04014">
        <property type="entry name" value="MazE_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01895">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00966">
        <property type="entry name" value="SpoVT_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109755">
        <property type="entry name" value="PhoU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="1">
        <location>
            <begin position="8"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="331" mass="36081" checksum="3B49DAF0BD5118EE" modified="2005-11-08" version="1">METRKVQVTGGSTYTVSLPKGWATENDISGGSVVEFHPEEESLLLTPRHGDDKIEGTLDITGLEGDELMRAVVTMYVSGFDIITLETAQVSAAQRRTIRDATQGLVGLEVIGETSGRVRLQDLLDSSELSMHNAVTRMRLVATTMLADAVTALLDDDDELAADVVQRDDDVDRLWSMVSRVFRSVLRDPSAAATIGLDRETCFDYHSSARQLERIGDHAAKIATHAETLSTPPTDAADAIRDLHDEATEVVDMGMDALLEDDSDRATRLAHDARTRIANVDELTRRVDDEIRELDPQQAQLLGLVVDSLSRSADYGGNIAETALQNAAPKP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IS14</accession>
    <name>GUAAB_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GMP synthase [glutamine-hydrolyzing] subunit B</fullName>
            <ecNumber evidence="1">6.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">GMP synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">guaAB</name>
        <name type="ordered locus">NP_1970A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the synthesis of GMP from XMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>XMP + L-glutamine + ATP + H2O = GMP + L-glutamate + AMP + diphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B).</text>
    </comment>
    <dbReference type="EC" id="6.3.5.2" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322706.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IS14"/>
    <dbReference type="SMR" id="Q3IS14"/>
    <dbReference type="STRING" id="348780.NP_1970A"/>
    <dbReference type="EnsemblBacteria" id="CAI49076">
        <property type="protein sequence ID" value="CAI49076"/>
        <property type="gene ID" value="NP_1970A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702783"/>
    <dbReference type="KEGG" id="nph:NP_1970A"/>
    <dbReference type="eggNOG" id="arCOG00085">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014340_0_0_2"/>
    <dbReference type="OrthoDB" id="33844at2157"/>
    <dbReference type="UniPathway" id="UPA00189">
        <property type="reaction ID" value="UER00296"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003921">
        <property type="term" value="F:GMP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01997">
        <property type="entry name" value="GMP_synthase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000208">
        <property type="entry name" value="GMP synthase [glutamine-hydrolyzing] subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00345">
        <property type="entry name" value="GMP_synthase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001674">
        <property type="entry name" value="GMP_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026598">
        <property type="entry name" value="GMP_synthase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025777">
        <property type="entry name" value="GMPS_ATP_PPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00884">
        <property type="entry name" value="guaA_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11922:SF2">
        <property type="entry name" value="GMP SYNTHASE [GLUTAMINE-HYDROLYZING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11922">
        <property type="entry name" value="GMP SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00958">
        <property type="entry name" value="GMP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54810">
        <property type="entry name" value="GMP synthetase C-terminal dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51553">
        <property type="entry name" value="GMPS_ATP_PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0332">GMP biosynthesis</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000048381" description="GMP synthase [glutamine-hydrolyzing] subunit B">
        <location>
            <begin position="1"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="GMPS ATP-PPase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="29"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00345"/>
        </source>
    </evidence>
    <sequence length="305" mass="33885" checksum="1A44728E2E63514E" modified="2005-11-08" version="1">MVEPTAFIDEKIAEIADDVGDANAVIALSGGVDSSTAAALAYEAIGDQLTPVYVDTGLMRKGETDQIRDTFDYMDSLRIIDARDRFLDALSGITDPEEKRHAIGEQFIREFETVARDVDADYLVQGTIYPDRIESEGTIKSHHNVGGLPEVVDFEGIVEPMRDLYKDEVREVARELDLESIIAERMPFPGPGLAIRVLGEVTDEKLAVAREANHVVEEELEEYEPWQALAAVLGKATGVKGDNRVHGWVVSVRSVESRDGMTARAQELDWETLQRIQSRITGENENVARVVYDVTHKPPATIEYE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IS15</accession>
    <name>PYRG_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CTP synthase</fullName>
            <ecNumber evidence="1">6.3.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cytidine 5'-triphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Cytidine triphosphate synthetase</fullName>
            <shortName evidence="1">CTP synthetase</shortName>
            <shortName evidence="1">CTPS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">UTP--ammonia ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrG</name>
        <name type="ordered locus">NP_1968A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>UTP + L-glutamine + ATP + H2O = CTP + L-glutamate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:26426"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>UTP + NH4(+) + ATP = CTP + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:16597"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Allosterically activated by GTP, when glutamine is the substrate; GTP has no effect on the reaction when ammonia is the substrate. The allosteric effector GTP functions by stabilizing the protein conformation that binds the tetrahedral intermediate(s) formed during glutamine hydrolysis. Inhibited by the product CTP, via allosteric rather than competitive inhibition.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">CTPSs have evolved a hybrid strategy for distinguishing between UTP and CTP. The overlapping regions of the product feedback inhibitory and substrate sites recognize a common feature in both compounds, the triphosphate moiety. To differentiate isosteric substrate and product pyrimidine rings, an additional pocket far from the expected kinase/ligase catalytic site, specifically recognizes the cytosine and ribose portions of the product inhibitor.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CTP synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.2" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322705.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IS15"/>
    <dbReference type="SMR" id="Q3IS15"/>
    <dbReference type="STRING" id="348780.NP_1968A"/>
    <dbReference type="MEROPS" id="C26.964"/>
    <dbReference type="EnsemblBacteria" id="CAI49075">
        <property type="protein sequence ID" value="CAI49075"/>
        <property type="gene ID" value="NP_1968A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703086"/>
    <dbReference type="KEGG" id="nph:NP_1968A"/>
    <dbReference type="eggNOG" id="arCOG00063">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011675_5_0_2"/>
    <dbReference type="OrthoDB" id="52769at2157"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00277"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097268">
        <property type="term" value="C:cytoophidium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003883">
        <property type="term" value="F:CTP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019856">
        <property type="term" value="P:pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03113">
        <property type="entry name" value="CTPS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01746">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000009">
        <property type="entry name" value="CTP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000002">
        <property type="entry name" value="CTP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01227">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004468">
        <property type="entry name" value="CTP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017456">
        <property type="entry name" value="CTP_synthase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033828">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003792">
        <property type="entry name" value="PRK05380.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00337">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550">
        <property type="entry name" value="CTP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550:SF0">
        <property type="entry name" value="CTP SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06418">
        <property type="entry name" value="CTP_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000266278" description="CTP synthase">
        <location>
            <begin position="1"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-1" evidence="1">
        <location>
            <begin position="303"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Amidoligase domain" evidence="1">
        <location>
            <begin position="1"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; for glutamine hydrolysis" evidence="1">
        <location>
            <position position="390"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="514"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="516"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="26"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="158"/>
            <end position="160"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="197"/>
            <end position="202"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="197"/>
            <end position="202"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="391"/>
            <end position="394"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="414"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="471"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01227"/>
        </source>
    </evidence>
    <sequence length="548" mass="61118" checksum="295513F4C07BB3C9" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-01-23" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ITN6</accession>
    <name>SECE_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein translocase subunit SecE</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Protein transport protein Sec61 gamma subunit homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">secE</name>
        <name type="ordered locus">NP_0812A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Essential subunit of the Sec protein translocation channel SecYEG. Clamps together the 2 halves of SecY. May contact the channel plug during translocation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the Sec protein translocase complex. Heterotrimer consisting of SecY (alpha), SecG (beta) and SecE (gamma) subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. May interact with SecDF, and other proteins may be involved.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SecE/SEC61-gamma family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322133.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ITN6"/>
    <dbReference type="SMR" id="Q3ITN6"/>
    <dbReference type="STRING" id="348780.NP_0812A"/>
    <dbReference type="EnsemblBacteria" id="CAI48497">
        <property type="protein sequence ID" value="CAI48497"/>
        <property type="gene ID" value="NP_0812A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703201"/>
    <dbReference type="KEGG" id="nph:NP_0812A"/>
    <dbReference type="eggNOG" id="arCOG02204">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_191921_2_0_2"/>
    <dbReference type="OrthoDB" id="52835at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.820">
        <property type="entry name" value="Preprotein translocase SecE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00422">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023391">
        <property type="entry name" value="Prot_translocase_SecE_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008158">
        <property type="entry name" value="Translocase_Sec61-g"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001901">
        <property type="entry name" value="Translocase_SecE/Sec61-g"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006910">
        <property type="entry name" value="PRK09400.1-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00327">
        <property type="entry name" value="secE_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103456">
        <property type="entry name" value="Preprotein translocase SecE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0653">Protein transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0811">Translocation</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000273139" description="Protein translocase subunit SecE">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00422"/>
        </source>
    </evidence>
    <sequence length="57" mass="6119" checksum="C4FAEDA1CC6262C4" modified="2005-11-08" version="1">MKTPTDLTSYIRVLKLASTPTWDEFSKVATIAGLGILLVGFIGFVIFSIMTFVPGGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-05-14" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>W8ZLT3</accession>
    <name>W8ZLT3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NP_6161D</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CDN71463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W8ZLT3"/>
    <dbReference type="EnsemblBacteria" id="CDN71463">
        <property type="protein sequence ID" value="CDN71463"/>
        <property type="gene ID" value="NP_6161D"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_6161D"/>
    <dbReference type="HOGENOM" id="CLU_217075_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CDN71463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="32" mass="3496" checksum="3B3DC2485DFC4C77" modified="2014-05-14" version="1">MITPIITTTLKYPAAVYEARKLQPPHDDASPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETA2</accession>
    <name>A0A1U7ETA2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Zinc metalloprotease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">NP_0030A</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="14 16">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14 16">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the peptidase M50B family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321745.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETA2"/>
    <dbReference type="STRING" id="348780.NP_0030A"/>
    <dbReference type="EnsemblBacteria" id="CAI48106">
        <property type="protein sequence ID" value="CAI48106"/>
        <property type="gene ID" value="NP_0030A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700742"/>
    <dbReference type="KEGG" id="nph:NP_0030A"/>
    <dbReference type="eggNOG" id="arCOG00607">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037123_1_1_2"/>
    <dbReference type="OrthoDB" id="12044at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04801">
        <property type="entry name" value="CBS_pair_peptidase_M50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06164">
        <property type="entry name" value="S2P-M50_SpoIVFB_CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008915">
        <property type="entry name" value="Peptidase_M50"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016483">
        <property type="entry name" value="UCP006404_Pept_M50_CBS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39188">
        <property type="entry name" value="MEMBRANE-ASSOCIATED ZINC METALLOPROTEASE M50B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39188:SF3">
        <property type="entry name" value="STAGE IV SPORULATION PROTEIN FB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02163">
        <property type="entry name" value="Peptidase_M50"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006404">
        <property type="entry name" value="UCP006404_Pept_M50_CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 17" id="KW-0129">CBS domain</keyword>
    <keyword evidence="3 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 14 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 14 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 14 19" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4 14" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9 14 16" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="120"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="215"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="18">
        <location>
            <begin position="265"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="18">
        <location>
            <begin position="329"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="active site" evidence="15">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR006404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR006404-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR006404-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAI48106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="396" mass="42820" checksum="C46A1FE844E6BA6A" modified="2017-05-10" version="1">MFKSFRVGSIADIPIKLDITLLLILPVLAFLIGSGMGEAVEAFEQIGAAFESPELLVAGWTPWLLGFVAAVGLFVCVLLHELGHAAVARHYDYGIASITLWLLGGVARPEKQPEQWNHEFWIAIGGPVVSVALGIGCYLVYGFAGAEPVQSVLGVLTADSLRFLFGYLALLNIVLAAFNMLPAFPLDGGRVMRALLGRTRPFTEATKTAVAVGKFFAILFGIFGLLMFNPFLVAIAFFVYIAAAAEGRQTVLEAAFRGVRVADVMTPANEVRTVETTATLDAILDRMFEERHTGYPVVEGGKLVGIVTLADIRNVHPEKRSETRVADVMSEDLEAVSPDTEAMDAMRQLAQHSVGRLVVTDEFGNLAGLLTRSDLVTAMNVVQEKRLSRGEEAAPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETA8</accession>
    <name>A0A1U7ETA8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">SAMP-activating enzyme E1</fullName>
            <ecNumber evidence="5">2.7.7.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ubaA</name>
        <name evidence="5" type="synonym">moeB</name>
        <name evidence="5" type="ordered locus">NP_0052A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.-" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321756.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETA8"/>
    <dbReference type="STRING" id="348780.NP_0052A"/>
    <dbReference type="EnsemblBacteria" id="CAI48117">
        <property type="protein sequence ID" value="CAI48117"/>
        <property type="gene ID" value="NP_0052A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702934"/>
    <dbReference type="KEGG" id="nph:NP_0052A"/>
    <dbReference type="eggNOG" id="arCOG01676">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013325_10_0_2"/>
    <dbReference type="OrthoDB" id="7915at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008641">
        <property type="term" value="F:ubiquitin-like modifier activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00757">
        <property type="entry name" value="ThiF_MoeB_HesA_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000033">
        <property type="entry name" value="Adenylyltransferase and sulfurtransferase MOCS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004281">
        <property type="entry name" value="PRK05690.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF047750">
        <property type="entry name" value="SAMPActivE1UbaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF102">
        <property type="entry name" value="ADENYLYLTRANSFERASE AND SULFURTRANSFERASE MOCS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="4">
        <location>
            <begin position="14"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI48117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="270" mass="28523" checksum="B5737BFB90D1561D" modified="2017-05-10" version="1">MTDLSLSADQLDRYSRHIIMDDVGPEGQKRLLDANVLCIGAGGLGSPIIQYLAAAGVGTLGIADDDVVERSNLQRQVIHGDDDVGQPKVESAAAFVEALNPDVTVEPHETRVTADNIDGLLAEYDVVVDGSDNFATRYLVNDACTLAGVPFAHGAILRFEGQITTFEATPEGPCYRCLFPEAPEPGTVPDCATAGVLGVLPGTVGCIQATEAVKLVLGHGETLDGRMLFYDAADMSFEEIPVEKRPDCPVCGDGGVESVHDVDYESTCAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETI2</accession>
    <name>A0A1U7ETI2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">rio2</name>
        <name evidence="14" type="synonym">pkn1</name>
        <name evidence="14" type="ordered locus">NP_0248A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321854.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETI2"/>
    <dbReference type="STRING" id="348780.NP_0248A"/>
    <dbReference type="EnsemblBacteria" id="CAI48215">
        <property type="protein sequence ID" value="CAI48215"/>
        <property type="gene ID" value="NP_0248A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700801"/>
    <dbReference type="KEGG" id="nph:NP_0248A"/>
    <dbReference type="eggNOG" id="arCOG01181">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018693_1_0_2"/>
    <dbReference type="OrthoDB" id="50101at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05144">
        <property type="entry name" value="RIO2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000647">
        <property type="entry name" value="Serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000052">
        <property type="entry name" value="Serine/threonine-protein kinase RIO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030484">
        <property type="entry name" value="Rio2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015285">
        <property type="entry name" value="RIO2_wHTH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018934">
        <property type="entry name" value="RIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000687">
        <property type="entry name" value="RIO_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45852">
        <property type="entry name" value="SER/THR-PROTEIN KINASE RIO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45852:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE RIO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01163">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09202">
        <property type="entry name" value="Rio2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00090">
        <property type="entry name" value="RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RIO kinase" evidence="13">
        <location>
            <begin position="65"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAI48215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="302" mass="34753" checksum="CF5CDDBBF7495389" modified="2017-05-10" version="1">MVQNVATQMAELEEPDFHLLSGIEHGMRFSRWVAEGKLPKFSRLDEEELTYRLDRCMDRELIERKTIQYTGYRLTFEGYDALALRTFSERESIDGVGSPLGVGKESDVYEARSFRPLALKFHREGYTNFREVQKEREYTADNEHRSWFYTARKAAEREYDALETLYPEVSVPRPVDQNRHAIVMEKVDGVELSRAKLDADQVVGVAELILEEMATAYNAGYVHADMSEYNVFVAADGVTIFDWPQSVPTEHENSDELLVRDVENLLGHFERKYPSETPAVDERALADALAADEFDSLRAFEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETJ7</accession>
    <name>A0A1U7ETJ7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ACT domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0300A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321879.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETJ7"/>
    <dbReference type="STRING" id="348780.NP_0300A"/>
    <dbReference type="EnsemblBacteria" id="CAI48241">
        <property type="protein sequence ID" value="CAI48241"/>
        <property type="gene ID" value="NP_0300A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700816"/>
    <dbReference type="KEGG" id="nph:NP_0300A"/>
    <dbReference type="eggNOG" id="arCOG04396">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094842_0_0_2"/>
    <dbReference type="OrthoDB" id="60296at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd04886">
        <property type="entry name" value="ACT_ThrD-II-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044561">
        <property type="entry name" value="ACT_ThrD-II-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="174" mass="18457" checksum="A5E2E66709BDDFE0" modified="2017-05-10" version="1">MSEATVRAYTVRLELVDEPGELLGALEPIADNGGNLLSIFHERGNVTPRGHIPVEVDLEATEAQFETIVDALREAGVNVIQAGAERYSEELTVVLVGHLVETDLSDTLQQIQECGGASVADVSLSAADGTDEESSARLRLVASEGHTSDALETIQDVADKKDLRVVEPLTGVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETK6</accession>
    <name>A0A1U7ETK6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HdaI-type histone deacetylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">hda1</name>
        <name evidence="2" type="ordered locus">NP_0318A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321888.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETK6"/>
    <dbReference type="STRING" id="348780.NP_0318A"/>
    <dbReference type="EnsemblBacteria" id="CAI48250">
        <property type="protein sequence ID" value="CAI48250"/>
        <property type="gene ID" value="NP_0318A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702547"/>
    <dbReference type="KEGG" id="nph:NP_0318A"/>
    <dbReference type="eggNOG" id="arCOG00324">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007727_8_2_2"/>
    <dbReference type="OrthoDB" id="147549at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004407">
        <property type="term" value="F:histone deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09992">
        <property type="entry name" value="HDAC_classII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF10">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="1">
        <location>
            <begin position="18"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="336" mass="36505" checksum="3F25A35CCC82C635" modified="2017-05-10" version="1">MRFGYREACLEHDTGSRHPETADRLRAIRRGLKRRHGVEYRDGRLAERDAIEAVHDADYVADIEAFMADGGGTWDADTVAVEETWEAALASAGLASWAVDEAIGGADGRQTPFAIGRPPGHHAEIDEAMGFCFFNNAAVAVQQALDAEDSGVDSAVIFDWDVHHGNGTQDIFYDSDDVFYASIHERGLFPGTGDVLETGGPDARNTILNVPLPGGSGDAEYRAVMEQLLRPALDRFEPDLFVVSAGFDAHKHDPISRMRVTTEGYGYLTATVRDIAADHDAPLAFVLEGGYGLDPLADSVGIVHEIFDGREPMEPGGDVRSKSKRVINDVRDAHDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETN7</accession>
    <name>A0A1U7ETN7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0394A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321926.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETN7"/>
    <dbReference type="STRING" id="348780.NP_0394A"/>
    <dbReference type="EnsemblBacteria" id="CAI48288">
        <property type="protein sequence ID" value="CAI48288"/>
        <property type="gene ID" value="NP_0394A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700840"/>
    <dbReference type="KEGG" id="nph:NP_0394A"/>
    <dbReference type="eggNOG" id="arCOG04695">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119850_0_0_2"/>
    <dbReference type="OrthoDB" id="191500at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043832">
        <property type="entry name" value="DUF5809"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19125">
        <property type="entry name" value="DUF5809"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="145" mass="16564" checksum="4EAFF77676B555A8" modified="2017-05-10" version="1">MDEQQTRGFLQPETWADAEARYEALGPTAQTMVREVARAMSFDREEYDERVTPAVVETARQTLFAEQLTVRVADRETFNVWREESTYDVRRAGSDNVDRVAWHVSPAAEAATAATFQNEPDAAVGTVRRMAFNRLYRDRLTGDEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETR5</accession>
    <name>A0A1U7ETR5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0426A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321940.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETR5"/>
    <dbReference type="STRING" id="348780.NP_0426A"/>
    <dbReference type="EnsemblBacteria" id="CAI48304">
        <property type="protein sequence ID" value="CAI48304"/>
        <property type="gene ID" value="NP_0426A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700852"/>
    <dbReference type="KEGG" id="nph:NP_0426A"/>
    <dbReference type="eggNOG" id="arCOG04742">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2802087_0_0_2"/>
    <dbReference type="OrthoDB" id="190239at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89447">
        <property type="entry name" value="AbrB/MazE/MraZ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="65" mass="7371" checksum="B8273C5D54930AC1" modified="2017-05-10" version="1">MVRKKKLSPSGAKDEDGEYHNVHVNIHEDELAVAGMEIGDEVFVRVRDGKIIIQKADPEDVEHEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETS3</accession>
    <name>A0A1U7ETS3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0466A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321960.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETS3"/>
    <dbReference type="STRING" id="348780.NP_0466A"/>
    <dbReference type="EnsemblBacteria" id="CAI48324">
        <property type="protein sequence ID" value="CAI48324"/>
        <property type="gene ID" value="NP_0466A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700866"/>
    <dbReference type="KEGG" id="nph:NP_0466A"/>
    <dbReference type="HOGENOM" id="CLU_1718266_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="152" mass="16279" checksum="9E910DC7D9D0AB10" modified="2017-05-10" version="1">MPSSISLRDRTAAARATLVGVVYLLAFQVAFLVMPFTTEALWLWPLGLAEAFGRGLEGVVAFFTGGEWLLAFVIPIAVFVLGFAAAYSYLVDRNPVPAVIAFGFLAVFYLTEVAFAGQFEYAILYLAAMPVAALLAVAGHLLSPQRRTAVAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETS4</accession>
    <name>A0A1U7ETS4_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Leucine--tRNA ligase</fullName>
            <ecNumber evidence="2 10">6.1.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">leuS</name>
        <name evidence="17" type="ordered locus">NP_0452A</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Leu) + L-leucine + ATP = L-leucyl-tRNA(Leu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11688"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="2 10"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321953.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETS4"/>
    <dbReference type="STRING" id="348780.NP_0452A"/>
    <dbReference type="EnsemblBacteria" id="CAI48317">
        <property type="protein sequence ID" value="CAI48317"/>
        <property type="gene ID" value="NP_0452A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702882"/>
    <dbReference type="KEGG" id="nph:NP_0452A"/>
    <dbReference type="eggNOG" id="arCOG00809">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004427_0_0_2"/>
    <dbReference type="OrthoDB" id="23906at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004823">
        <property type="term" value="F:leucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006429">
        <property type="term" value="P:leucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07958">
        <property type="entry name" value="Anticodon_Ia_Leu_BEm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00812">
        <property type="entry name" value="LeuRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000002">
        <property type="entry name" value="Leucine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000003">
        <property type="entry name" value="Leucine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000056">
        <property type="entry name" value="Leucine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2320.20">
        <property type="entry name" value="Class I aminoacyl-tRNA synthetases (RS)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.720">
        <property type="entry name" value="leucyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00049_B">
        <property type="entry name" value="Leu_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002302">
        <property type="entry name" value="Leu-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025709">
        <property type="entry name" value="Leu_tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00396">
        <property type="entry name" value="leuS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43740:SF2">
        <property type="entry name" value="LEUCINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43740">
        <property type="entry name" value="LEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13603">
        <property type="entry name" value="tRNA-synt_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00985">
        <property type="entry name" value="TRNASYNTHLEU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 11 17" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methionyl/Leucyl tRNA synthetase" evidence="15">
        <location>
            <begin position="40"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Leucyl-tRNA synthetase editing" evidence="16">
        <location>
            <begin position="223"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="13">
        <location>
            <begin position="408"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="14">
        <location>
            <begin position="644"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="285"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="859"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="285"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="NCBIfam" id="TIGR00396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF09334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF13603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAI48317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="880" mass="99261" checksum="BE6D74F9CD0E26B8" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETT8</accession>
    <name>A0A1U7ETT8_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF8159 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0520A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321987.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETT8"/>
    <dbReference type="STRING" id="348780.NP_0520A"/>
    <dbReference type="EnsemblBacteria" id="CAI48351">
        <property type="protein sequence ID" value="CAI48351"/>
        <property type="gene ID" value="NP_0520A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700883"/>
    <dbReference type="KEGG" id="nph:NP_0520A"/>
    <dbReference type="eggNOG" id="arCOG06310">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1850687_0_0_2"/>
    <dbReference type="OrthoDB" id="290983at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058473">
        <property type="entry name" value="DUF8159"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26490">
        <property type="entry name" value="DUF8159"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8159" evidence="2">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="130" mass="14296" checksum="700C68E5C2047199" modified="2017-05-10" version="1">MAASDDIGARLERQLMSHGVYVERYVREGETLHVEYETAAAGLTKGDIGNVCAELREAVNAGWDPSDCRFWVFDTNGGFCGDWRVRAGWFRALDRGDISETDFSTLVLSTREPAAEPPAERPAFGGGKTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETU6</accession>
    <name>A0A1U7ETU6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA mismatch repair protein MutS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">mutS1b</name>
        <name evidence="11" type="ordered locus">NP_0538A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">This protein is involved in the repair of mismatches in DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321996.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETU6"/>
    <dbReference type="STRING" id="348780.NP_0538A"/>
    <dbReference type="EnsemblBacteria" id="CAI48360">
        <property type="protein sequence ID" value="CAI48360"/>
        <property type="gene ID" value="NP_0538A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702951"/>
    <dbReference type="KEGG" id="nph:NP_0538A"/>
    <dbReference type="eggNOG" id="arCOG02896">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002472_4_0_2"/>
    <dbReference type="OrthoDB" id="146065at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03284">
        <property type="entry name" value="ABC_MutS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000870">
        <property type="entry name" value="MutS protein homolog 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1420.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.10">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.110">
        <property type="entry name" value="MutS, connector domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005748">
        <property type="entry name" value="DNA_mismatch_repair_MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007695">
        <property type="entry name" value="DNA_mismatch_repair_MutS-lik_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017261">
        <property type="entry name" value="DNA_mismatch_repair_MutS/MSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007861">
        <property type="entry name" value="DNA_mismatch_repair_MutS_clamp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016151">
        <property type="entry name" value="DNA_mismatch_repair_MutS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007860">
        <property type="entry name" value="DNA_mmatch_repair_MutS_con_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01070">
        <property type="entry name" value="mutS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003810">
        <property type="entry name" value="PRK05399.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF34">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01624">
        <property type="entry name" value="MutS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05188">
        <property type="entry name" value="MutS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05190">
        <property type="entry name" value="MutS_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037677">
        <property type="entry name" value="DNA_mis_repair_Msh6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55271">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53150">
        <property type="entry name" value="DNA repair protein MutS, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="10">
        <location>
            <begin position="683"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="820"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="831"/>
            <end position="841"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="NCBIfam" id="TIGR01070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI48360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="877" mass="95856" checksum="764211E3C7782353" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETV5</accession>
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        </submittedName>
    </protein>
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        <name evidence="4" type="primary">oxdhA1</name>
        <name evidence="4" type="ordered locus">NP_0560A</name>
    </gene>
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        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.4.4" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322007.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETV5"/>
    <dbReference type="STRING" id="348780.NP_0560A"/>
    <dbReference type="EnsemblBacteria" id="CAI48371">
        <property type="protein sequence ID" value="CAI48371"/>
        <property type="gene ID" value="NP_0560A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703003"/>
    <dbReference type="KEGG" id="nph:NP_0560A"/>
    <dbReference type="eggNOG" id="arCOG01054">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029393_1_0_2"/>
    <dbReference type="OrthoDB" id="25266at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003863">
        <property type="term" value="F:branched-chain 2-oxo acid dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009083">
        <property type="term" value="P:branched-chain amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02000">
        <property type="entry name" value="TPP_E1_PDC_ADC_BCADC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050771">
        <property type="entry name" value="Alpha-ketoacid_DH_E1_comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017596">
        <property type="entry name" value="PdhA/BkdA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03181">
        <property type="entry name" value="PDH_E1_alph_x"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43380">
        <property type="entry name" value="2-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43380:SF1">
        <property type="entry name" value="2-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dehydrogenase E1 component" evidence="3">
        <location>
            <begin position="39"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="327"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="359"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="374" mass="41583" checksum="DA7925C9D48164DD" modified="2017-05-10" version="1">MSTLQRDPRDRVQVLDEDGQVVAEDGVPDIDDETLLEMYRTMRLARHFDERAVSLQRQGRMGTFPPMSGQEAAQVGSALALDADDWVFPSYREHAAAYVRGIDLDQTLQYWMGDERGSQTAAANVFPVAVPIATQVLHATGVAWAADLKGDDIVSLCYFGDGATSEGDFHEGLNFAGVFDLPAVFFCNNNQWAISVPREQQSASATLAQKAHAYGFEGVLVDGMDPLAVYKVTREAVEKAHAADPSRDDAPGRACRPTLIEAIQYRFGAHTTADDPSVYRDDDEVERWKQKDPIPRLELFLRDRGLLDDERVAAIEQSVTDEVADAVEAAESTERPAPDSMFDNVYADRPPELDAQQRQLDRLRNRYGDEELLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETV8</accession>
    <name>A0A1U7ETV8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DEAD/DEAH box helicase</fullName>
            <ecNumber evidence="7">3.6.4.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">NP_0564A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.4.-" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48373.3"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETV8"/>
    <dbReference type="STRING" id="348780.NP_0564A"/>
    <dbReference type="EnsemblBacteria" id="CAI48373">
        <property type="protein sequence ID" value="CAI48373"/>
        <property type="gene ID" value="NP_0564A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702914"/>
    <dbReference type="KEGG" id="nph:NP_0564A"/>
    <dbReference type="eggNOG" id="arCOG00553">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006553_3_1_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.10">
        <property type="entry name" value="Sec63 N-terminal domain-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004179">
        <property type="entry name" value="Sec63-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057842">
        <property type="entry name" value="WH_MER3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF6">
        <property type="entry name" value="DNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14520">
        <property type="entry name" value="HHH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02889">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23445">
        <property type="entry name" value="WHD_SNRNP200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00973">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="38"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="238"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48373.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="794" mass="86549" checksum="B1B997016F8EC350" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETV9</accession>
    <name>A0A1U7ETV9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TIGR00725 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0552A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322003.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETV9"/>
    <dbReference type="STRING" id="348780.NP_0552A"/>
    <dbReference type="EnsemblBacteria" id="CAI48367">
        <property type="protein sequence ID" value="CAI48367"/>
        <property type="gene ID" value="NP_0552A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700890"/>
    <dbReference type="KEGG" id="nph:NP_0552A"/>
    <dbReference type="eggNOG" id="arCOG02431">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107614_1_1_2"/>
    <dbReference type="OrthoDB" id="9570at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005268">
        <property type="entry name" value="CHP00725"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041164">
        <property type="entry name" value="LDcluster4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052341">
        <property type="entry name" value="LOG_family_nucleotidases"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00725">
        <property type="entry name" value="TIGR00725 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43393">
        <property type="entry name" value="CYTOKININ RIBOSIDE 5'-MONOPHOSPHATE PHOSPHORIBOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43393:SF3">
        <property type="entry name" value="LYSINE DECARBOXYLASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18306">
        <property type="entry name" value="LDcluster4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102405">
        <property type="entry name" value="MCP/YpsA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="153" mass="15512" checksum="93CD11562AFB83E4" modified="2017-05-10" version="1">MRVSVIGGSSVGDESAAIAESVGQTLAEHGHTVVCGGLGGVMEAVCRGAHNAEPPAETIGILPGRDRTAANDWVTTPIATGLGNARNVVVVRNGDAVIAVDGAAGTLSELGHALDLGRPVAGLRTHDIELDGFQPVETPTEAVEYVERAVGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU14</accession>
    <name>A0A1U7EU14_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Phenylalanine--tRNA ligase alpha subunit</fullName>
            <ecNumber evidence="11">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Phenylalanyl-tRNA synthetase alpha subunit</fullName>
            <shortName evidence="11">PheRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 13" type="primary">pheS</name>
        <name evidence="13" type="ordered locus">NP_0696A</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 2 magnesium ions per tetramer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="11"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322075.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU14"/>
    <dbReference type="STRING" id="348780.NP_0696A"/>
    <dbReference type="EnsemblBacteria" id="CAI48439">
        <property type="protein sequence ID" value="CAI48439"/>
        <property type="gene ID" value="NP_0696A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703025"/>
    <dbReference type="KEGG" id="nph:NP_0696A"/>
    <dbReference type="eggNOG" id="arCOG00410">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025086_2_2_2"/>
    <dbReference type="OrthoDB" id="372178at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00496">
        <property type="entry name" value="PheRS_alpha_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00282">
        <property type="entry name" value="Phe_tRNA_synth_alpha2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004529">
        <property type="entry name" value="Phe-tRNA-synth_IIc_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022917">
        <property type="entry name" value="Phe_tRNA_ligase_alpha_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00468">
        <property type="entry name" value="pheS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003210">
        <property type="entry name" value="PRK04172.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538:SF40">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01047">
        <property type="entry name" value="MarR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 11 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="8 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="12">
        <location>
            <begin position="247"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>L-phenylalanine</name>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="384"/>
            <end position="386"/>
        </location>
        <ligand>
            <name>L-phenylalanine</name>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="424"/>
        </location>
        <ligand>
            <name>L-phenylalanine</name>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="426"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>ligand shared with heterodimeric partner</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="450"/>
        </location>
        <ligand>
            <name>L-phenylalanine</name>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAI48439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="504" mass="55639" checksum="C26ABE67A764A575" modified="2017-05-10" version="1">MRLPETQLALLEAASATETKTVAQLAEALGEDPAAVTRAAFELETEGLVAVEEHVAEEVAVTEEGDRYRDDGLPEVRLYRAAVDAGADEEPVELGRLLGSAALDGPEVDIALSNFARKGYGHIDSGKVDAAPDPTHDPNADPEAEALDAIANDNAGYLDAEVLDRLESRGLIERTETTVRSVTLTDDGVTALMEGIETAETVGAVTPELLASGEWEDVEFAEYNVEADAEPVNGGKEHILRQTANRVKDVLVGMGFQEMEGPHADADFWINDCLFMPQDHPARTHWDRFALDVPGMDELPADLVERVEDAHRNGVGSEGDGYHSPWDEDFARAVALRGHTTSLSMRYLSGHAVGDLEPPQRYFSVEKVYRNDTLDPTHLLEFYQIEGWVMAEDLSVRDLMGTFTEFYEQFGITDLQFKPHYNPYTEPSFELFGEHPETGELIEIGNSGIFREEVLSPLGVDCDVMAWGLALERLLMLMYGFEDIRDVHGTLCDLELLRDVEVVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU16</accession>
    <name>A0A1U7EU16_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Beta-lactamase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_0670A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322062.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU16"/>
    <dbReference type="STRING" id="348780.NP_0670A"/>
    <dbReference type="EnsemblBacteria" id="CAI48426">
        <property type="protein sequence ID" value="CAI48426"/>
        <property type="gene ID" value="NP_0670A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702677"/>
    <dbReference type="KEGG" id="nph:NP_0670A"/>
    <dbReference type="eggNOG" id="arCOG00541">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009673_5_1_2"/>
    <dbReference type="OrthoDB" id="40950at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16295">
        <property type="entry name" value="TTHA0252-CPSF-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050698">
        <property type="entry name" value="MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203:SF52">
        <property type="entry name" value="MRNA 3-END PROCESSING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10996">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01027">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="2">
        <location>
            <begin position="13"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-Casp" evidence="3">
        <location>
            <begin position="223"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="412" mass="44510" checksum="430D6F8AC90C26AA" modified="2017-05-10" version="1">MELQFLGGAGEIGRSAVLVNGSLLLDYGIQTATPPQYPVGDVEPDAVVVSHGHLDHAGAVPSLLSGDRRPPIHWTPPTRELALTLARDTLKLHGGSYRCPFTETAVKRVTQVSESHGYRESFEAAGHEVTLFDAGHIPGSAHVLVDDGETRLLYTGDFHTDDQRLVAGTSARPDADVVVCESTYADVDHDPRADVEDRFAQSVKTTLWEGGTVVVPAFAIGRTQEMLLVCEAHDIPCYVDGMGKQVTEMLRGYPGFVRDDEAFRRAKSHARFVTGRDGQRKRIAEENAAIVTTSGMLSGGPAMTYIPEIRAHPTNMVALTGYQVEGTPGRRLVETGRAEIDGRVMPVSAQIEQYDFSAHADREGLVSFLDPYRGGDTEALVVHGDRCADFAATLSERGLPASAPEVGETVHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU58</accession>
    <name>A0A1U7EU58_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0752A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322103.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU58"/>
    <dbReference type="STRING" id="348780.NP_0752A"/>
    <dbReference type="EnsemblBacteria" id="CAI48467">
        <property type="protein sequence ID" value="CAI48467"/>
        <property type="gene ID" value="NP_0752A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700952"/>
    <dbReference type="KEGG" id="nph:NP_0752A"/>
    <dbReference type="eggNOG" id="arCOG06312">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1998685_0_0_2"/>
    <dbReference type="OrthoDB" id="199125at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055954">
        <property type="entry name" value="DUF7532"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24376">
        <property type="entry name" value="DUF7532"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="124" mass="13734" checksum="038A9DAF0A06F390" modified="2017-05-10" version="1">MHFDQRTQAALRDAGLDTDEIADISTAVTESTAEAADDIEAFFEGLDTVYSDMDMAHSSADSPEHGLEYVDLYTHADELRGYIKFDGWGAYVEDGRVLSESVVELTLGPTVHDRVRFAADRDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUA2</accession>
    <name>A0A1U7EUA2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ubiquitin-like modifier protein SAMP2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">samp2</name>
        <name evidence="1" type="ordered locus">NP_0902A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322178.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUA2"/>
    <dbReference type="STRING" id="348780.NP_0902A"/>
    <dbReference type="EnsemblBacteria" id="CAI48542">
        <property type="protein sequence ID" value="CAI48542"/>
        <property type="gene ID" value="NP_0902A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700994"/>
    <dbReference type="KEGG" id="nph:NP_0902A"/>
    <dbReference type="eggNOG" id="arCOG00535">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114601_9_3_2"/>
    <dbReference type="OrthoDB" id="104640at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053752">
        <property type="entry name" value="SAM_domain_containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053833">
        <property type="entry name" value="SAMP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053834">
        <property type="entry name" value="SAMP2_halobacteria"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041919">
        <property type="entry name" value="SAMP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21965">
        <property type="entry name" value="SAMP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="MoaD/ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="66" mass="7044" checksum="E6DAABC9CA6F1293" modified="2017-05-10" version="1">MRVTCDIVGEDTRELDVDEAATYGDLLEAVGLSSHEASVLVDGSPVPEDRTVDSDSVRILRLIKGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUH2</accession>
    <name>A0A1U7EUH2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PQQ repeat protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1072A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322263.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUH2"/>
    <dbReference type="STRING" id="348780.NP_1072A"/>
    <dbReference type="EnsemblBacteria" id="CAI48627">
        <property type="protein sequence ID" value="CAI48627"/>
        <property type="gene ID" value="NP_1072A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701047"/>
    <dbReference type="KEGG" id="nph:NP_1072A"/>
    <dbReference type="eggNOG" id="arCOG02556">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027480_4_2_2"/>
    <dbReference type="OrthoDB" id="145878at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002372">
        <property type="entry name" value="PQQ_rpt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34512">
        <property type="entry name" value="CELL SURFACE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34512:SF30">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN ASSEMBLY FACTOR BAMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13360">
        <property type="entry name" value="PQQ_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyrrolo-quinoline quinone repeat" evidence="1">
        <location>
            <begin position="86"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrrolo-quinoline quinone repeat" evidence="1">
        <location>
            <begin position="324"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="444" mass="48766" checksum="A57B9029BFD6131D" modified="2017-05-10" version="1">MTDSPASPSSTRRRLLSAAGVAVTAGLAGCTGLGYAYRRDQSAFDPALVPYNETYPDDDGVTMFRQGLRRLGYYPDATVPDTVSVAWERPINEVGHTAAKASPRPTPDGETVLIPADTGELHALTPAGEERWSVQTDATNLGFHGTPLVVDGTAYIGGYDGAMYAFDVDSGQRRWKTSRWRLRGAIAIGSSPAYWDGVIYVVAEYNHPWQQPSGTMWALDAETGRPLWHDSRLWGMPHPSTAIDPATERLITGSNDGVCYCWEFPSMAFEWSFQTDGEIKGTIPTYDGGAFVGSWDGHLYRLDLEDGTEEWRFETGRVVMSNPGIGPDEGVVYFGSDDNRVYALDADSGEELWSTNVGGNVIGSLSVTQETVLVGSYDGHLYALRRDTGERRWRHPCRGHVTSEAVPHDGRIFVAERGVFENYWDDDEPTTFVEPGHAYALVGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUJ9</accession>
    <name>A0A1U7EUJ9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cobalt-factor-II C20-methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.130</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cbiL</name>
        <name evidence="5" type="synonym">cobI</name>
        <name evidence="5" type="ordered locus">NP_1122A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.130" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EnsemblBacteria" id="CAI48652">
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        <property type="gene ID" value="NP_1122A"/>
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        <property type="taxonomic scope" value="Archaea"/>
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        <property type="term" value="P:cobalamin biosynthetic process"/>
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        <property type="term" value="P:methylation"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="4pyrrol_Mease"/>
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        <property type="entry name" value="4pyrrole_Mease_sub1"/>
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        <location>
            <begin position="2"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR036427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI48652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="232" mass="24879" checksum="536ABD2A2D39FE7D" modified="2017-05-10" version="1">MTLYGVGLGPGNAGLITVRGREVLESADVVYTPGRLSRNVATEYVDESQLGDLDFPMTRDEEKLRRAWKAAAEAVAPVATDGTAAFVTLGDPNVYSTFGHLRRTLDAFHPAVDVEVVPGVSTVTAFATALDVEITAGSELALREADGGAAPVGPDRLVLFKVTDVPTTHEKLREAGYEVTYGRRLFMDGHESVITDDPAALAERDYYTVAYAERPEALEEPVTAAFEGVSDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUK8</accession>
    <name>A0A1U7EUK8_NATPD</name>
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        <submittedName>
            <fullName evidence="3">HTH domain protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ordered locus">NP_1142A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
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            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUK8"/>
    <dbReference type="STRING" id="348780.NP_1142A"/>
    <dbReference type="EnsemblBacteria" id="CAI48662">
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        <property type="gene ID" value="NP_1142A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701064"/>
    <dbReference type="KEGG" id="nph:NP_1142A"/>
    <dbReference type="eggNOG" id="arCOG01449">
        <property type="taxonomic scope" value="Archaea"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="entry name" value="DUF6293_C"/>
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    </dbReference>
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        <property type="entry name" value="WHD_DUF6293"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="12"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="DUF6293" evidence="2">
        <location>
            <begin position="146"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="250" mass="28411" checksum="B90FCAC68370DF35" modified="2017-05-10" version="1">MRSETELRPVQEVHVAPVGYEYDRIKRPALEYGADTLYLLRERERTELSYHDRLVTELTDRGMTVRSREVDLDDMYDVLGEVTTIVHGHDGDIVRVNVSSGPKLSTVGAALACMATDASGYHVHPETRAHPLDEQPRTEGMQFAEQLPSYPLETPTEDQVRVLDYIDRRDSETYTPKKSDLIEFAEDEQLDFLTRSEPANDKAKFALLNNRILEPLSANGYVHIEPVGRTKQVSLTDTGENALRAFRHKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUL3</accession>
    <name>A0A1U7EUL3_NATPD</name>
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        <submittedName>
            <fullName evidence="3">Isochorismatase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">entB2</name>
        <name evidence="3" type="ordered locus">NP_1170A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUL3"/>
    <dbReference type="STRING" id="348780.NP_1170A"/>
    <dbReference type="EnsemblBacteria" id="CAI48676">
        <property type="protein sequence ID" value="CAI48676"/>
        <property type="gene ID" value="NP_1170A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702676"/>
    <dbReference type="KEGG" id="nph:NP_1170A"/>
    <dbReference type="eggNOG" id="arCOG01943">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068979_8_4_2"/>
    <dbReference type="OrthoDB" id="9194at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="cysteine_hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="entry name" value="Isochorismatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050272">
        <property type="entry name" value="Isochorismatase-like_hydrls"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43540:SF6">
        <property type="entry name" value="ISOCHORISMATASE-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43540">
        <property type="entry name" value="PEROXYUREIDOACRYLATE/UREIDOACRYLATE AMIDOHYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="Isochorismatase-like hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase-like" evidence="2">
        <location>
            <begin position="8"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="191" mass="21117" checksum="669186D99A2F452B" modified="2017-05-10" version="1">MELNPETTAVVVVDMQNGFCHPDGSLYAPGSEAAITPCADLVADAREAGASVVFTRDVHPPEQFEDTHYYDEFDRWGEHVLEGSWDAELVAELSPRDDELVVVKHTYDAFHQTQLDGWLESHGINDLLICGTLANVCVLHTAGSAGLRDYRPILVEDAVGAIEDDHREYALDHAEWLFGDVYERGALSFDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUN3</accession>
    <name>A0A1U7EUN3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Chromosome segregation and condensation protein ScpA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">scpA</name>
        <name evidence="2" type="ordered locus">NP_1214A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322334.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUN3"/>
    <dbReference type="STRING" id="348780.NP_1214A"/>
    <dbReference type="EnsemblBacteria" id="CAI48698">
        <property type="protein sequence ID" value="CAI48698"/>
        <property type="gene ID" value="NP_1214A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701086"/>
    <dbReference type="KEGG" id="nph:NP_1214A"/>
    <dbReference type="eggNOG" id="arCOG02610">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070251_0_0_2"/>
    <dbReference type="OrthoDB" id="53244at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.580">
        <property type="entry name" value="Structural maintenance of chromosome 1. Chain E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003768">
        <property type="entry name" value="ScpA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023093">
        <property type="entry name" value="ScpA-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33969">
        <property type="entry name" value="SEGREGATION AND CONDENSATION PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33969:SF2">
        <property type="entry name" value="SEGREGATION AND CONDENSATION PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02616">
        <property type="entry name" value="SMC_ScpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="315" mass="35459" checksum="0109DFADAD327D8D" modified="2017-05-10" version="1">MTDDRRAATDGGAADEIPLDITGHGDTREATQLGDGDERDEPLSEFDIEEPGGDDDTVEPVELLVQLAEEGEIDPWDIDVVTVTDKFLDRLDEADLRTGGRALFYASVLLRMKSDELWNDDEPDDEPEAAPWEAPPGEEPPAGGDPFAELEREIDRRIERKDARGTPETLDELVRDLRERERETWWKESRSYDTSESPSGYSRGTQTLDYHDSDEFRMDEEPTAADVTERTHDEHMEDIINDVYVSLQQHYDRGREEVLFAEVRTAGGSTVDSYLGALFLAHRGQVRLRQDDLFGDLWIQDPTSAAAEGQAAADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUU6</accession>
    <name>A0A1U7EUU6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Riboflavin synthase</fullName>
            <ecNumber evidence="3 8">2.5.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ribE</name>
        <name evidence="11" type="synonym">ribC</name>
        <name evidence="11" type="ordered locus">NP_1356A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the dismutation of two molecules of 6,7-dimethyl-8-ribityllumazine, resulting in the formation of riboflavin and 5-amino-6-(D-ribitylamino)uracil.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.9" evidence="3 8"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322404.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUU6"/>
    <dbReference type="STRING" id="348780.NP_1356A"/>
    <dbReference type="EnsemblBacteria" id="CAI48769">
        <property type="protein sequence ID" value="CAI48769"/>
        <property type="gene ID" value="NP_1356A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703105"/>
    <dbReference type="KEGG" id="nph:NP_1356A"/>
    <dbReference type="eggNOG" id="arCOG04713">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034388_1_2_2"/>
    <dbReference type="OrthoDB" id="10084at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004746">
        <property type="term" value="F:riboflavin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00402">
        <property type="entry name" value="Riboflavin_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.20:FF:000003">
        <property type="entry name" value="Riboflavin synthase, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.20:FF:000004">
        <property type="entry name" value="Riboflavin synthase, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001783">
        <property type="entry name" value="Lumazine-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026017">
        <property type="entry name" value="Lumazine-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006767">
        <property type="entry name" value="PRK09289.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00187">
        <property type="entry name" value="ribE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21098:SF0">
        <property type="entry name" value="RIBOFLAVIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21098">
        <property type="entry name" value="RIBOFLAVIN SYNTHASE ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00677">
        <property type="entry name" value="Lum_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000498">
        <property type="entry name" value="Riboflavin_syn_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51177">
        <property type="entry name" value="LUMAZINE_BIND"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="6 11" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="Lumazine-binding" evidence="9">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="Lumazine-binding" evidence="10">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="repeat" description="Lumazine-binding" evidence="9">
        <location>
            <begin position="96"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Lumazine-binding" evidence="10">
        <location>
            <begin position="96"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="NCBIfam" id="TIGR00187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI48769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="197" mass="21163" checksum="FA057E0F9959BC20" modified="2017-05-10" version="1">MFTGIVEATGDVAAVEDTDEGRRIRVTAPFCDELEHGQSVAISGACLTVEAWDDESFELFLSEETLEKTYLDEVAVGDPVNLERALPADGRFDGHFVQGHVDGVAEVTAIEGTDGDDWTFGFSLPSSLAPYVVDKGSIAVDGISLTVAELRADDFSVAVIPATYELTTLSSKSVGDPVHIEVDVVAKYVERLTEGYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUY2</accession>
    <name>A0A1U7EUY2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">D-lactate dehydrogenase (cytochrome)</fullName>
            <ecNumber evidence="7">1.1.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">NP_1452A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAD-binding oxidoreductase/transferase type 4 family.</text>
    </comment>
    <dbReference type="EC" id="1.1.2.4" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322452.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUY2"/>
    <dbReference type="STRING" id="348780.NP_1452A"/>
    <dbReference type="EnsemblBacteria" id="CAI48817">
        <property type="protein sequence ID" value="CAI48817"/>
        <property type="gene ID" value="NP_1452A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702667"/>
    <dbReference type="KEGG" id="nph:NP_1452A"/>
    <dbReference type="eggNOG" id="arCOG00337">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010756_0_0_2"/>
    <dbReference type="OrthoDB" id="2837at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004458">
        <property type="term" value="F:D-lactate dehydrogenase (cytochrome) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008720">
        <property type="term" value="F:D-lactate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903457">
        <property type="term" value="P:lactate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="entry name" value="Vanillyl-alcohol Oxidase, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-bd_oxidored_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748:SF111">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="10">
        <location>
            <begin position="63"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="9">
        <location>
            <begin position="659"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="384"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="390"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI48817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="1011" mass="108714" checksum="2AB45A5B61A11A04" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUZ7</accession>
    <name>A0A1U7EUZ7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC-type transport system ATP-binding protein (Probable substrate branched-chain amino acids)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">livG3</name>
        <name evidence="5" type="synonym">abc07a2</name>
        <name evidence="5" type="ordered locus">NP_1470A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322461.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUZ7"/>
    <dbReference type="STRING" id="348780.NP_1470A"/>
    <dbReference type="EnsemblBacteria" id="CAI48826">
        <property type="protein sequence ID" value="CAI48826"/>
        <property type="gene ID" value="NP_1470A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702383"/>
    <dbReference type="KEGG" id="nph:NP_1470A"/>
    <dbReference type="eggNOG" id="arCOG00926">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OrthoDB" id="44250at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03219">
        <property type="entry name" value="ABC_Mj1267_LivG_branched"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051120">
        <property type="entry name" value="ABC_AA/LPS_Transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45772:SF2">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45772">
        <property type="entry name" value="CONSERVED COMPONENT OF ABC TRANSPORTER FOR NATURAL AMINO ACIDS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI48826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="243" mass="26414" checksum="79730DAC45E2F864" modified="2017-05-10" version="1">MTLLSIQNLTKRFGGLVAVNDVSFDIEAGSIVGLIGPNGSGKTTIFNCIMSIYDVTEGSIQFQGTDITDYKTHEVVNSGLARVSQESNPIARMSVAANIKLFTLPNSVTALSGGADDEEIYEIAARVGLEDALDKQPDSLPHADVRRLEIAKAIATEPELLLLDEPFAGLNQQEIREMSDQIRQLRDDGVTIVIIDHNMRGLMQLVERIMVINNGDWLADDTPEAIAENEAVQQAYLAGTETV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUZ8</accession>
    <name>A0A1U7EUZ8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">DUF21/CBS domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">cbs9</name>
        <name evidence="11" type="ordered locus">NP_1414A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUZ8"/>
    <dbReference type="STRING" id="348780.NP_1414A"/>
    <dbReference type="EnsemblBacteria" id="CAI48798">
        <property type="protein sequence ID" value="CAI48798"/>
        <property type="gene ID" value="NP_1414A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701142"/>
    <dbReference type="KEGG" id="nph:NP_1414A"/>
    <dbReference type="eggNOG" id="arCOG00626">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015237_4_1_2"/>
    <dbReference type="OrthoDB" id="9671at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04590">
        <property type="entry name" value="CBS_pair_CorC_HlyC_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000002">
        <property type="entry name" value="Magnesium/cobalt efflux protein CorC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002550">
        <property type="entry name" value="CNNM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044751">
        <property type="entry name" value="Ion_transp-like_CBS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005170">
        <property type="entry name" value="Transptr-assoc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22777">
        <property type="entry name" value="HEMOLYSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22777:SF17">
        <property type="entry name" value="UPF0053 PROTEIN SLL0260"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01595">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03471">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01091">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51846">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="CNNM transmembrane" evidence="10">
        <location>
            <begin position="26"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="9">
        <location>
            <begin position="234"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="9">
        <location>
            <begin position="300"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI48798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="457" mass="50588" checksum="368639E90BB2D38F" modified="2017-05-10" version="1">MGTLASNAALVVLQAAPDAGELPFSDTTVALLGSAVILLLLALSGFFSSSEIAMFSLPAHRVDALVADNIPHAETLKELKDDPHRLLVTILVGNNIVNIAMSSIATGLLSYYVSQSMAVLIATFGITALVLLFGESAPKSYAVENTESWALRISRPLKLSEYLLLPLIVTFDYLTRQVNRITGGRAEIESTYVTREEIRDIIETGERAGVLEEDEREMLQRIFRFTNTIAKEVMTPRLDMEAVPKDATIEEAIQTCVQSGHARVPVYEGSLDNVLGVVHISDLVRDDTYGEASDIELEDVIEETLHVPESKNIDELLAEMRENRLHMVIVIDEFGTTEGLVTMEDITEEIVGEILQADEEEPIEFVSDNEILVKGEVNIDEVNEKLDVAIPEGEEFETIAGFIFNRAGRLVEEGERIEYEGLEIRVERVENTRIMKARITKTDNREGDGEHEAAGDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV40</accession>
    <name>A0A1U7EV40_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DedA family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1586A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322518.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV40"/>
    <dbReference type="STRING" id="348780.NP_1586A"/>
    <dbReference type="EnsemblBacteria" id="CAI48884">
        <property type="protein sequence ID" value="CAI48884"/>
        <property type="gene ID" value="NP_1586A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701193"/>
    <dbReference type="KEGG" id="nph:NP_1586A"/>
    <dbReference type="eggNOG" id="arCOG03117">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1478870_0_0_2"/>
    <dbReference type="OrthoDB" id="204088at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="167" mass="16613" checksum="E6343AEE436BE042" modified="2017-05-10" version="1">MYETVVDGGISLVAQYGLVVLLIAFVLEGALVGKLIPTRALFVAAALVVGSDAVGLAAVAAVAVVGATLGQAVLFVAVRRTDCSVESLPGPTDAAGGAVVGWFDRWGLPAVAVSNALPVARGSLTVPVAMTDESVLRFCGSSVLGSSVYAVGLVAVAAGLDVLFSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV42</accession>
    <name>A0A1U7EV42_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Peroxiredoxin domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1584A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322517.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV42"/>
    <dbReference type="SMR" id="A0A1U7EV42"/>
    <dbReference type="STRING" id="348780.NP_1584A"/>
    <dbReference type="EnsemblBacteria" id="CAI48883">
        <property type="protein sequence ID" value="CAI48883"/>
        <property type="gene ID" value="NP_1584A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701192"/>
    <dbReference type="KEGG" id="nph:NP_1584A"/>
    <dbReference type="eggNOG" id="arCOG00316">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_14_2_2"/>
    <dbReference type="OrthoDB" id="165617at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016209">
        <property type="term" value="F:antioxidant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="1">
        <location>
            <begin position="2"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="177" mass="19334" checksum="ACDF320AC36E8CA3" modified="2017-05-10" version="1">MIREGETAPDFALPGVERGEPDVYELFRPIENGDAVLLWFVPSPFVPTATAELAAINRAGWHDCEGLQVWVVSMESIYAAAAYADDHGFPMAFLGDGASAADYYGVKQDEWEGHYNVPERAAFFIDDDWTVEAAWRRADAFERPSPSPVEVVAASVAEHVPAATADVSVEYDECFGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV65</accession>
    <name>A0A1U7EV65_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ArNOG05395 family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1628A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322539.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV65"/>
    <dbReference type="STRING" id="348780.NP_1628A"/>
    <dbReference type="EnsemblBacteria" id="CAI48905">
        <property type="protein sequence ID" value="CAI48905"/>
        <property type="gene ID" value="NP_1628A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701206"/>
    <dbReference type="KEGG" id="nph:NP_1628A"/>
    <dbReference type="eggNOG" id="arCOG04399">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090237_0_0_2"/>
    <dbReference type="OrthoDB" id="56502at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00092">
        <property type="entry name" value="HTH_CRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057161">
        <property type="entry name" value="DUF7839"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012318">
        <property type="entry name" value="HTH_CRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012015">
        <property type="entry name" value="UCP_HTH_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43704">
        <property type="entry name" value="BSR5907 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43704:SF2">
        <property type="entry name" value="HTH CRP-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25211">
        <property type="entry name" value="DUF7839"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004955">
        <property type="entry name" value="HTH_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00419">
        <property type="entry name" value="HTH_CRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH crp-type" evidence="1">
        <location>
            <begin position="19"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="257" mass="26431" checksum="3A39D68C9BD57F84" modified="2017-05-10" version="1">MVDVLDNKRSATKFRVLVEIADRQPAVNQGEIADAVGVTSQAVSEYIRELVDEGFVEKEARSRYRVTKEGVDWLFQQAADVSRFAEHVTEDVLGSMQEDAAFAADDIAAGETVSLSISDGLLYATPGDSGPATGVATTDAETGDVVGVTGFAGIIDLDPGSVTVFQVPPIRSGDPSGSPELADTAADADVVAAAGVEGVAALRDVDADPDVTFAAGEVAADAAGRGLDVVVVATTDLAGRVTDALRDAGIAYEVDDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV75</accession>
    <name>A0A1U7EV75_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">WD40/YVTN domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1648A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322549.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV75"/>
    <dbReference type="STRING" id="348780.NP_1648A"/>
    <dbReference type="EnsemblBacteria" id="CAI48915">
        <property type="protein sequence ID" value="CAI48915"/>
        <property type="gene ID" value="NP_1648A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701210"/>
    <dbReference type="KEGG" id="nph:NP_1648A"/>
    <dbReference type="eggNOG" id="arCOG02560">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_686276_0_0_2"/>
    <dbReference type="OrthoDB" id="167723at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011048">
        <property type="entry name" value="Haem_d1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051200">
        <property type="entry name" value="Host-pathogen_enzymatic-act"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47197:SF3">
        <property type="entry name" value="DIHYDRO-HEME D1 DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47197">
        <property type="entry name" value="PROTEIN NIRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51004">
        <property type="entry name" value="C-terminal (heme d1) domain of cytochrome cd1-nitrite reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="428" mass="46041" checksum="6D0B22F93929DAD9" modified="2017-05-10" version="1">MERTVRRRTVLTVLASSGSVLVAGCSDDDGTETPETGDSGDGTDDGGNKAARADGDGDTDEGAENGDEPGSEGLLYAFAPDRAVAIAPEAGEVVEDLTDSIDPDLAGADWGDARLTHDRSRLFIVEADRNQLGAIDLKHREFLGWVDIGAGPTHAYNPVEGEIWAHADGEGRLYVVDTETLEVTDVIQSGLEEGGHGKLLTHEALQPIGFATNTDDSYGHVIDLESRERIDSISTGETGGTHYLMYAPESEVIYFERSGGDDMPYFDADTYEEVGRLDINGGLSLSPDTEMLGVWDDDTVYFVDATDRDSEILGSVTLEGRGPDDLDYFEEDDTLYAIVTNTTADEATVINVDEYAVESHIEVGEIDQDGHHLHRSGELGGDYYITPSGADGTIPVVDVENRELLHEVQVSDGVDTVCYVGDSSGAWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV99</accession>
    <name>A0A1U7EV99_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Serine--tRNA ligase</fullName>
            <ecNumber evidence="12">6.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Seryl-tRNA synthetase</fullName>
            <shortName evidence="12">SerRS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Seryl-tRNA(Ser/Sec) synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12 17" type="primary">serS</name>
        <name evidence="17" type="ordered locus">NP_1692A</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>tRNA(Sec) + L-serine + ATP = L-seryl-tRNA(Sec) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42580"/>
            <dbReference type="Rhea" id="RHEA-COMP:9742"/>
            <dbReference type="Rhea" id="RHEA-COMP:10128"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>tRNA(Ser) + L-serine + ATP = L-seryl-tRNA(Ser) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12292"/>
            <dbReference type="Rhea" id="RHEA-COMP:9669"/>
            <dbReference type="Rhea" id="RHEA-COMP:9703"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer. The tRNA molecule binds across the dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="12">Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.11" evidence="12"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322570.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV99"/>
    <dbReference type="STRING" id="348780.NP_1692A"/>
    <dbReference type="EnsemblBacteria" id="CAI48937">
        <property type="protein sequence ID" value="CAI48937"/>
        <property type="gene ID" value="NP_1692A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703207"/>
    <dbReference type="KEGG" id="nph:NP_1692A"/>
    <dbReference type="eggNOG" id="arCOG00403">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023797_0_1_2"/>
    <dbReference type="OrthoDB" id="35932at2157"/>
    <dbReference type="UniPathway" id="UPA00906">
        <property type="reaction ID" value="UER00895"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016260">
        <property type="term" value="P:selenocysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006434">
        <property type="term" value="P:seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00770">
        <property type="entry name" value="SerRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.40">
        <property type="entry name" value="Serine-tRNA synthetase, tRNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00176">
        <property type="entry name" value="Ser_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015866">
        <property type="entry name" value="Ser-tRNA-synth_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042103">
        <property type="entry name" value="SerRS_1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033729">
        <property type="entry name" value="SerRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00414">
        <property type="entry name" value="serS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43697:SF1">
        <property type="entry name" value="SERINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43697">
        <property type="entry name" value="SERYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02403">
        <property type="entry name" value="Seryl_tRNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001529">
        <property type="entry name" value="Ser-tRNA-synth_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00981">
        <property type="entry name" value="TRNASYNTHSER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="tRNA-binding arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 12 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12 17" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="16">
        <location>
            <begin position="138"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="44"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="242"/>
            <end position="244"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 14">
        <location>
            <begin position="273"/>
            <end position="275"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="289"/>
            <end position="292"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 13">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 14">
        <location>
            <begin position="369"/>
            <end position="372"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="403"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="405"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAI48937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="456" mass="51610" checksum="3E75C042D6EBAEEE" modified="2017-05-10" version="1">MLSRQFVRENPEEVRRALDTKGVDVDLDRILDIDEEWRELKAEGDDLRRQRNEVSSEIGKLKRDGEEEAAAEAIERSQELKEEIEAVEDRADELESELEAALLRLPNVPHPDAPVGDDESDNVEIDRWGFDDRRSLPDEVVPHYDLGEELDILDFERGAKVSGGGFYFAKGEGARLEHALVQFMLDVHRDEHGYTDVFPPLPVNSASMRGTGQFPKFVEDAYRIGGDNDDPYDDDDLWLLPTAEVPVTNMYRDEILLDDDLPLKQQAYSPNFRREAGEHGTETRGIVRVHQFNKVEMVNFVRPENSYDRLEGLLGEATDVLERLGLPYRVLEMCTGDMGFTQAKKYDVEVWAPGDDMEDGPDVGGRWLEVSSVSNFEDFQARRAHIQYRPEQHESAEYIHTLNASGLAVPRVVVAILEYYQNDDGTVDVPEPLQPYMGGTDVIDGQDPVGESALGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVE5</accession>
    <name>A0A1U7EVE5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ABC-type transport system periplasmic substrate-binding protein (Probable substrate phosphate/phosphonate)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">phnD1</name>
        <name evidence="1" type="synonym">abc17s</name>
        <name evidence="1" type="ordered locus">NP_1748A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322598.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVE5"/>
    <dbReference type="STRING" id="348780.NP_1748A"/>
    <dbReference type="EnsemblBacteria" id="CAI48965">
        <property type="protein sequence ID" value="CAI48965"/>
        <property type="gene ID" value="NP_1748A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702422"/>
    <dbReference type="KEGG" id="nph:NP_1748A"/>
    <dbReference type="eggNOG" id="arCOG01805">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051472_6_0_2"/>
    <dbReference type="OrthoDB" id="146127at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01071">
        <property type="entry name" value="PBP2_PhnD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35841">
        <property type="entry name" value="PHOSPHONATES-BINDING PERIPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35841:SF1">
        <property type="entry name" value="PHOSPHONATES-BINDING PERIPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12974">
        <property type="entry name" value="Phosphonate-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="307" mass="32915" checksum="6C1919D7358725C8" modified="2017-05-10" version="1">MRRRTYLTAAGGLFAAGLAGCTDEEPTGNGNGDTGEEPMTVGEWADGHIEFALPPFQDAEELTAEYEPVFEWLEDGFDGVDTVTATPTTSYSAVVESVVNGHTEMANLSPIIYALAAEDGVNPLAVNWSHGSEAYHTYIATRAETDIETLADIQGHTIAMVDPLSTSGGLFPRYMLDEAGLDAGDVDTEPGDFDIDWAFGHGAALDALQAGHVDAAAYGDFEHPDDDDIVRVAESDPIPFDPVVAKPETPDAVQEALIDRLLETPEDVLEAHRIDRFGEVEPGTYDPVRNVAEAMGVDIADLEEDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVI1</accession>
    <name>A0A1U7EVI1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Probable F420-dependent oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_1902A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322674.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVI1"/>
    <dbReference type="STRING" id="348780.NP_1902A"/>
    <dbReference type="EnsemblBacteria" id="CAI49042">
        <property type="protein sequence ID" value="CAI49042"/>
        <property type="gene ID" value="NP_1902A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701299"/>
    <dbReference type="KEGG" id="nph:NP_1902A"/>
    <dbReference type="eggNOG" id="arCOG02410">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027853_5_0_2"/>
    <dbReference type="OrthoDB" id="194060at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01097">
        <property type="entry name" value="Tetrahydromethanopterin_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="entry name" value="Luciferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050564">
        <property type="entry name" value="F420-G6PD/mer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023909">
        <property type="entry name" value="F420_NP1902A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04024">
        <property type="entry name" value="F420_NP1902A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43244">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43244:SF1">
        <property type="entry name" value="5,10-METHYLENETETRAHYDROMETHANOPTERIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="Bacterial luciferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Luciferase-like" evidence="2">
        <location>
            <begin position="10"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="332" mass="36463" checksum="525810AD96A9C924" modified="2017-05-10" version="1">MPRDVFLPVAAQPSVDALVEQAQLGEQLGYDTAWLPETWGRDAVTTLTCIAQGTDDIDIGTSIMPVYSRSPALIGQSAATLQEVSDGRFRLGLGPSGPVVIENWHGVDFGNPLRRTRETVDVVKQVLAGEEVTYDGEYFDLEGFRLRCDPPEPTPPVDAAGMGPKAVELAGRFADGWHALLFTRDGIRDRLEDFDRGSEMGDRDRDDQRVTLSLTCCVLDDAEEAKSLVKQHTAFYIGGMGTFYRDSLARQGHEETAHEIYDHWQDGDQAAAMDALDDDLLEEIAVWGTPDTAPDHFERFADIDGVESASVSFPRGADLDQIESTMRALAPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVJ9</accession>
    <name>A0A1U7EVJ9_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histidine kinase</fullName>
            <ecNumber evidence="3">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">NP_1912A</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVJ9"/>
    <dbReference type="STRING" id="348780.NP_1912A"/>
    <dbReference type="EnsemblBacteria" id="CAI49047">
        <property type="protein sequence ID" value="CAI49047"/>
        <property type="gene ID" value="NP_1912A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_1912A"/>
    <dbReference type="eggNOG" id="arCOG02329">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02364">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02369">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06796">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010703_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000156">
        <property type="term" value="F:phosphorelay response regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007234">
        <property type="term" value="P:osmosensory signaling via phosphorelay pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050351">
        <property type="entry name" value="BphY/WalK/GraS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013656">
        <property type="entry name" value="PAS_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42878:SF7">
        <property type="entry name" value="SENSOR HISTIDINE KINASE GLRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42878">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13185">
        <property type="entry name" value="GAF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08448">
        <property type="entry name" value="PAS_4"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="PAS" evidence="14">
        <location>
            <begin position="300"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="14">
        <location>
            <begin position="565"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="14">
        <location>
            <begin position="679"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="13">
        <location>
            <begin position="808"/>
            <end position="1017"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAI49047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="1020" mass="111579" checksum="2261BDDE428009E9" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVL2</accession>
    <name>A0A1U7EVL2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Probable S-adenosylmethionine-dependent methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_1930A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322688.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVL2"/>
    <dbReference type="STRING" id="348780.NP_1930A"/>
    <dbReference type="EnsemblBacteria" id="CAI49056">
        <property type="protein sequence ID" value="CAI49056"/>
        <property type="gene ID" value="NP_1930A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701309"/>
    <dbReference type="KEGG" id="nph:NP_1930A"/>
    <dbReference type="eggNOG" id="arCOG04340">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106195_0_0_2"/>
    <dbReference type="OrthoDB" id="173767at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43464">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43464:SF19">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS O-METHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13489">
        <property type="entry name" value="Methyltransf_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="206" mass="23060" checksum="234470FD03EAEB03" modified="2017-05-10" version="1">MGKSEKIRYDAVLQRIPETTETILDIGCARHSEEKRESANLHEYLITNTECHVQGIDVLEEEIEKMRNEGYDVQVGDAEIFESETEFDVIVAGEVIEHLKNIGKFIRTAESNLAVGGKLIITTPNPDGFAYFRKALFNQPNNPTHTCWVDPKNLEQLISITETDLQLNEWTYLPPVGGVSMVLWKTGFSRAASPGYVAELSLDSTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVP1</accession>
    <name>A0A1U7EVP1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC-type transport system ATP-binding protein (Probable substrate heme)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ccmA</name>
        <name evidence="6" type="synonym">abc29a</name>
        <name evidence="6" type="ordered locus">NP_2038A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322739.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVP1"/>
    <dbReference type="STRING" id="348780.NP_2038A"/>
    <dbReference type="EnsemblBacteria" id="CAI49110">
        <property type="protein sequence ID" value="CAI49110"/>
        <property type="gene ID" value="NP_2038A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702451"/>
    <dbReference type="KEGG" id="nph:NP_2038A"/>
    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OrthoDB" id="40048at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43335">
        <property type="entry name" value="ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43335:SF4">
        <property type="entry name" value="ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="4"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI49110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="239" mass="25538" checksum="CC78313989D80DCE" modified="2017-05-10" version="1">MSFVSVAGLSKRFGSHLAVDDVSFELTAGETAVLFGPNGAGKTTLIRMLASLARPSDGTIRIDGQPLVGDDAARGDIGVLTHETLLYEELTARENLRLHARLHGVDEAICEELLETVGLAARGSERVSGFSHGMAKRVSLARALVHDPALLLLDEPYTGLDQTSLKRMTTVLSELADRTVLASTHDLERGVELADRILFMREGRLVGDAAADEFETVEGAVDRYETLCGGEPAVGGALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVU5</accession>
    <name>A0A1U7EVU5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">3-isopropylmalate dehydratase</fullName>
            <ecNumber evidence="7">4.2.1.33</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">leuD</name>
        <name evidence="14" type="ordered locus">NP_2192A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate</text>
            <dbReference type="Rhea" id="RHEA:32287"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="EC" id="4.2.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterodimer of LeuC and LeuD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the LeuD family. LeuD type 1 subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the LeuD family. LeuD type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.33" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322815.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVU5"/>
    <dbReference type="STRING" id="348780.NP_2192A"/>
    <dbReference type="EnsemblBacteria" id="CAI49187">
        <property type="protein sequence ID" value="CAI49187"/>
        <property type="gene ID" value="NP_2192A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702881"/>
    <dbReference type="KEGG" id="nph:NP_2192A"/>
    <dbReference type="eggNOG" id="arCOG02230">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081378_0_0_2"/>
    <dbReference type="OrthoDB" id="6505at2157"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009316">
        <property type="term" value="C:3-isopropylmalate dehydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01577">
        <property type="entry name" value="IPMI_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004431">
        <property type="entry name" value="3-IsopropMal_deHydase_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033940">
        <property type="entry name" value="IPMI_Swivel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050075">
        <property type="entry name" value="LeuD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00171">
        <property type="entry name" value="leuD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002458">
        <property type="entry name" value="PRK01641.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43345:SF5">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43345">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT 2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="10 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="13">
        <location>
            <begin position="11"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="205"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAI49187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="214" mass="23457" checksum="14481452CEB2372B" modified="2017-05-10" version="1">MADETEIPSVKHVDGSGIPVRGNDIDTDQIIPARFMKVVTFDGLGEFAFFDQRFDEEDNEKDHPFNEDQYQDANVMVVNANFGCGSSREHAPQALVRWGIDAIIGESFAEIFAGNCLALGVPTVTADTETIEALQNWVEDNPDGDIEVDVEAETVTYGDTTVDVTVDDAQRKALVEGIWDTTALMKSNTEAIEATAESLPYVEDPEPRKGHADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVY5</accession>
    <name>A0A1U7EVY5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Molybdopterin synthase catalytic subunit</fullName>
            <ecNumber evidence="1">2.8.1.12</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">moaE</name>
        <name evidence="1" type="ordered locus">NP_2274A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.8.1.12" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322855.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVY5"/>
    <dbReference type="STRING" id="348780.NP_2274A"/>
    <dbReference type="EnsemblBacteria" id="CAI49228">
        <property type="protein sequence ID" value="CAI49228"/>
        <property type="gene ID" value="NP_2274A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702929"/>
    <dbReference type="KEGG" id="nph:NP_2274A"/>
    <dbReference type="eggNOG" id="arCOG00533">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088141_0_0_2"/>
    <dbReference type="OrthoDB" id="45235at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030366">
        <property type="term" value="F:molybdopterin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00756">
        <property type="entry name" value="MoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.40">
        <property type="entry name" value="Molybdopterin biosynthesis MoaE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036563">
        <property type="entry name" value="MoaE_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003448">
        <property type="entry name" value="Mopterin_biosynth_MoaE"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011061">
        <property type="entry name" value="PRK14493.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23404">
        <property type="entry name" value="MOLYBDOPTERIN SYNTHASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02391">
        <property type="entry name" value="MoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54690">
        <property type="entry name" value="Molybdopterin synthase subunit MoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="262" mass="28514" checksum="E1007376E33E2C2C" modified="2017-05-10" version="1">MYTTVVVGSDADAAAERLVSTLDGRVGVVREAADPADGSTAVGATTYTLGDGWRADGGDLSVDGALDRLAPDHDYALVVGVDGVRGPTVVAGDYDDDPENLLAAAERAADLDAEQIQDGLQETEPYETLESLVAAVKRSPDADYAGAIATFTGRVRAKEDADDERTRFLEFEKYDAVADERMDTIATELEQRDGVFEVLLHHRTGRVPDGEDIVFVVVLAGHRDEAFRTVEDGINRLKDEVPLFKKEVTEAETFWVHERDDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVZ3</accession>
    <name>A0A1U7EVZ3_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">N5-carboxyaminoimidazole ribonucleotide synthase</fullName>
            <shortName evidence="5 7">N5-CAIR synthase</shortName>
            <ecNumber evidence="5 7">6.3.4.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5 7">5-(carboxyamino)imidazole ribonucleotide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 7 9" type="primary">purK</name>
        <name evidence="9" type="ordered locus">NP_2286A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).</text>
    </comment>
    <comment type="function">
        <text evidence="7">Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO(3)- to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 7">
            <text>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + hydrogencarbonate + ATP = 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:19317"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58730"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5 7">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 7">Belongs to the PurK/PurT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.18" evidence="5 7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322861.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVZ3"/>
    <dbReference type="STRING" id="348780.NP_2286A"/>
    <dbReference type="EnsemblBacteria" id="CAI49234">
        <property type="protein sequence ID" value="CAI49234"/>
        <property type="gene ID" value="NP_2286A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703072"/>
    <dbReference type="KEGG" id="nph:NP_2286A"/>
    <dbReference type="eggNOG" id="arCOG01597">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011534_0_2_2"/>
    <dbReference type="OrthoDB" id="9299at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00942"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034028">
        <property type="term" value="F:5-(carboxyamino)imidazole ribonucleotide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004638">
        <property type="term" value="F:phosphoribosylaminoimidazole carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01928">
        <property type="entry name" value="PurK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003135">
        <property type="entry name" value="ATP-grasp_carboxylate-amine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005875">
        <property type="entry name" value="PurK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040686">
        <property type="entry name" value="PurK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054350">
        <property type="entry name" value="PurT/PurK_preATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004679">
        <property type="entry name" value="PRK06019.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004680">
        <property type="entry name" value="PRK06019.1-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01161">
        <property type="entry name" value="purK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11609:SF5">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11609">
        <property type="entry name" value="PURINE BIOSYNTHESIS PROTEIN 6/7, PUR6/7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02222">
        <property type="entry name" value="ATP-grasp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17769">
        <property type="entry name" value="PurK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22660">
        <property type="entry name" value="RS_preATP-grasp-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 5 7" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="8">
        <location>
            <begin position="112"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="182"/>
            <end position="185"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="266"/>
            <end position="267"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI49234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="384" mass="41057" checksum="A6AB0DD3E26A5603" modified="2017-05-10" version="1">MRHQTPAATVGVVGGGQLGRMMGEAAGPLGVELVVSDPTPDCPAAPVVRDQIVADFDDYDAIHELAERADVLTFEIELADPDEMAAVSEATDTPVHPTPETLRTIQDKLVQNRALDDAGIPIPAYRQVDSVADLRAAGEALGWPLMLKAREGGYDGRGNRPVEGPEEAPQAFEAVGGAALAEELIDFERELAVMGVRGADETCAYPVTETVHEAEILRELVAPARVDDAVRERAREVAFDVLEMLDGRGVFGIELFETADGGILVNEIAPRPHNSGHWTIEGAGCSQFEQHVRAVLGWPLGSAEARSPAVTRNILGDVDQPEAASLAGTGEVLSTPSASLHWYGKREVRPLRKMGHVTATDADANRGDLLQTARELDDALTFET</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWB0</accession>
    <name>A0A1U7EWB0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ArsR family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2558A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWB0"/>
    <dbReference type="STRING" id="348780.NP_2558A"/>
    <dbReference type="EnsemblBacteria" id="CAI49370">
        <property type="protein sequence ID" value="CAI49370"/>
        <property type="gene ID" value="NP_2558A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_2558A"/>
    <dbReference type="eggNOG" id="arCOG03065">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124803_4_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12840">
        <property type="entry name" value="HTH_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="16"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="123" mass="14184" checksum="DD92BC09EE592F22" modified="2017-05-10" version="1">MPIVHTSVEGEQTIEDILDTIGDQHARDVLAAISREPRSAKELADECDLSLPTIYRRLEVLESNDLVVERTAVSGDGNHYNIYECNFDSTVIQLEDDEYKVRIYRRENLPDRFTQLWDDLGKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWH8</accession>
    <name>A0A1U7EWH8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UspA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2652A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323042.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWH8"/>
    <dbReference type="STRING" id="348780.NP_2652A"/>
    <dbReference type="EnsemblBacteria" id="CAI49417">
        <property type="protein sequence ID" value="CAI49417"/>
        <property type="gene ID" value="NP_2652A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701512"/>
    <dbReference type="KEGG" id="nph:NP_2652A"/>
    <dbReference type="eggNOG" id="arCOG03051">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_20_0_2"/>
    <dbReference type="OrthoDB" id="157454at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="1">
        <location>
            <begin position="53"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="124" mass="12573" checksum="F2665E67821798CF" modified="2017-05-10" version="1">MGTQYLVATDSVHTTAAACDYLDPRLAADDTVTVATVADGSRDGGDATNVATVRLAGHEVTTTELDDEDVETAVLGAATERSADVLIIGPHAGEPEAGPAVGRTARNIIEGATVPVVVVPLSAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWI2</accession>
    <name>A0A1U7EWI2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">M50 family metalloprotease</fullName>
            <ecNumber evidence="2">3.4.24.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2704A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.4.24.-" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323068.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWI2"/>
    <dbReference type="STRING" id="348780.NP_2704A"/>
    <dbReference type="EnsemblBacteria" id="CAI49443">
        <property type="protein sequence ID" value="CAI49443"/>
        <property type="gene ID" value="NP_2704A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701531"/>
    <dbReference type="KEGG" id="nph:NP_2704A"/>
    <dbReference type="eggNOG" id="arCOG00614">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099718_0_0_2"/>
    <dbReference type="OrthoDB" id="86131at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052348">
        <property type="entry name" value="Metallopeptidase_M50B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35864">
        <property type="entry name" value="ZINC METALLOPROTEASE MJ0611-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35864:SF1">
        <property type="entry name" value="ZINC METALLOPROTEASE YWHC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="191"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="215" mass="22494" checksum="C2F07644415FECD2" modified="2017-05-10" version="1">MTVDVAEIRFSEVELRDLAIAWVALGVAFSIFIFVATGRDVFPDITGAILSPQFATVFVLSLVTVGVGFLLHELAHKVVAIRFGQAAAFRADYPMLALCVGAAFAGFLFAAPGAVHHRGRITPRENGLISVAGPVTNIVLVGVFLPLVFVGGFVGAVGQLGVIINAFLAAFNMIPFGPLDGKSVLAWSKPVFGAVFTASVLLAVGSFLFVGFPSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWK2</accession>
    <name>A0A1U7EWK2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">START domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2750A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323091.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWK2"/>
    <dbReference type="SMR" id="A0A1U7EWK2"/>
    <dbReference type="STRING" id="348780.NP_2750A"/>
    <dbReference type="EnsemblBacteria" id="CAI49466">
        <property type="protein sequence ID" value="CAI49466"/>
        <property type="gene ID" value="NP_2750A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701545"/>
    <dbReference type="KEGG" id="nph:NP_2750A"/>
    <dbReference type="eggNOG" id="arCOG04604">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112936_1_1_2"/>
    <dbReference type="OrthoDB" id="10357at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07820">
        <property type="entry name" value="SRPBCC_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019587">
        <property type="entry name" value="Polyketide_cyclase/dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10604">
        <property type="entry name" value="Polyketide_cyc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="160" mass="18031" checksum="BE9DF5AE865CE7E3" modified="2017-05-10" version="1">MATYHRETHVRASFEDVWEFHATTDGLEALTPSFLGLTVESVTGPDGEPDPEVLAAGSRIEMSMRPLGVAPQQRWTSVITHRERDGDTGSFRDEMVDGPFTEWEHTHRFRADGDGTVVSDTVRYEFPGGPAGRLVSPLGVVGFEPMFRYRHRRTRQLLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWP9</accession>
    <name>A0A1U7EWP9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">YbaK domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ybaK</name>
        <name evidence="2" type="ordered locus">NP_2868A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323150.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWP9"/>
    <dbReference type="STRING" id="348780.NP_2868A"/>
    <dbReference type="EnsemblBacteria" id="CAI49525">
        <property type="protein sequence ID" value="CAI49525"/>
        <property type="gene ID" value="NP_2868A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701576"/>
    <dbReference type="KEGG" id="nph:NP_2868A"/>
    <dbReference type="eggNOG" id="arCOG04332">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094875_0_0_2"/>
    <dbReference type="OrthoDB" id="27691at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04333">
        <property type="entry name" value="ProX_deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.960.10">
        <property type="entry name" value="YbaK/aminoacyl-tRNA synthetase-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036754">
        <property type="entry name" value="YbaK/aa-tRNA-synt-asso_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007214">
        <property type="entry name" value="YbaK/aa-tRNA-synth-assoc-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30411">
        <property type="entry name" value="CYTOPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30411:SF1">
        <property type="entry name" value="CYTOPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04073">
        <property type="entry name" value="tRNA_edit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55826">
        <property type="entry name" value="YbaK/ProRS associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YbaK/aminoacyl-tRNA synthetase-associated" evidence="1">
        <location>
            <begin position="27"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="158" mass="16659" checksum="6C5E7A4476527813" modified="2017-05-10" version="1">MHPRTAAFREHAEDQYGLEIDVHEFPEGTKTAADAADAVGCDLAQIASGIVFDVEGDLVVVVTSGANRVSEPKLAAECNVDTASVSMADADDIKETLGWAIGGVPPFCHETDVPVYMDETLTQHDDVWAAAGTPEAVFPIPPAKLRELADAAVVDVAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWQ0</accession>
    <name>A0A1U7EWQ0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2896A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323164.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWQ0"/>
    <dbReference type="EnsemblBacteria" id="CAI49539">
        <property type="protein sequence ID" value="CAI49539"/>
        <property type="gene ID" value="NP_2896A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701587"/>
    <dbReference type="KEGG" id="nph:NP_2896A"/>
    <dbReference type="eggNOG" id="arCOG02893">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2475902_0_0_2"/>
    <dbReference type="OrthoDB" id="191000at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055809">
        <property type="entry name" value="DUF7385"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24110">
        <property type="entry name" value="DUF7385"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="76" mass="8412" checksum="958747FE02583CEF" modified="2017-05-10" version="1">MDIDTDELLASLTPREENPAIKSYQNTVSVACPACEEPFDDLVVCKQNPTSLNLSKQLDLCVGVEDDQAFIFTHKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWR4</accession>
    <name>A0A1U7EWR4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Receiver box response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">NP_2906A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323169.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWR4"/>
    <dbReference type="STRING" id="348780.NP_2906A"/>
    <dbReference type="EnsemblBacteria" id="CAI49544">
        <property type="protein sequence ID" value="CAI49544"/>
        <property type="gene ID" value="NP_2906A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701592"/>
    <dbReference type="KEGG" id="nph:NP_2906A"/>
    <dbReference type="eggNOG" id="arCOG02601">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114810_0_0_2"/>
    <dbReference type="OrthoDB" id="86314at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032993">
        <property type="term" value="C:protein-DNA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000156">
        <property type="term" value="F:phosphorelay response regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17574">
        <property type="entry name" value="REC_OmpR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013971">
        <property type="entry name" value="HalX_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="REGULATOR OF RPOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111:SF1">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR ORR33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08663">
        <property type="entry name" value="HalX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="7">
        <location>
            <begin position="9"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="6">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="202" mass="22861" checksum="D1427F4B8BCC5CB2" modified="2017-05-10" version="1">MADGDDKPTVLVVEDERALIELYVRWLENDYEVLTATGGEEALEQFRDDIDVALLDRLMPGMSGDEVLEHLREEVPDCKVAMVTAVEPDFDVISMGFDDYLTKPVEREELVETVEGLLSRTAFDEIEQELYALSSKQAALRSSKPKEELDESEEFSELQERIDELRGDLDAAMPDMEDDDFVAMVRDIEDGEDDAERGGDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWR7</accession>
    <name>A0A1U7EWR7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2884A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323158.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWR7"/>
    <dbReference type="STRING" id="348780.NP_2884A"/>
    <dbReference type="EnsemblBacteria" id="CAI49533">
        <property type="protein sequence ID" value="CAI49533"/>
        <property type="gene ID" value="NP_2884A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701583"/>
    <dbReference type="KEGG" id="nph:NP_2884A"/>
    <dbReference type="eggNOG" id="arCOG04663">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098519_0_0_2"/>
    <dbReference type="OrthoDB" id="342532at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="218" mass="23868" checksum="E3BB34C54AAFB727" modified="2017-05-10" version="1">MTADGGGGARAAEGDDILAALFRNSRLNAIIAWLLVAVLAGALLESVLDRDLLWILFVSATAVVVLLPPAAYFDWRTMLPWELLVVALLPVLVRGLFGGELGTFGYYFSVAALALLIIVELHMFTELRLTHWFAVALVVMTTLASVAAWAVVRWILDRHFGTEFLSTPGMSQDAANAALMGEFLWVTLAGLLAGILFDSYFRHRGRRLGRHLRGVMGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWU9</accession>
    <name>A0A1U7EWU9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PadR family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3002A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323216.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWU9"/>
    <dbReference type="STRING" id="348780.NP_3002A"/>
    <dbReference type="EnsemblBacteria" id="CAI49592">
        <property type="protein sequence ID" value="CAI49592"/>
        <property type="gene ID" value="NP_3002A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701623"/>
    <dbReference type="KEGG" id="nph:NP_3002A"/>
    <dbReference type="eggNOG" id="arCOG00005">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151709_0_0_2"/>
    <dbReference type="OrthoDB" id="190025at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator PadR N-terminal" evidence="1">
        <location>
            <begin position="11"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="92" mass="11189" checksum="8DA68E3246E0DD35" modified="2017-05-10" version="1">MYDLTGFQRDLLYVIAGREEPHGLAIKEELEDYYEKEIHHGRLYPNLDTLVEKGLVEKGQRDRRTNFYTLTRRGRREIEARRDWEDEYVDIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWX7</accession>
    <name>A0A1U7EWX7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7350 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3028A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323228.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWX7"/>
    <dbReference type="STRING" id="348780.NP_3028A"/>
    <dbReference type="EnsemblBacteria" id="CAI49605">
        <property type="protein sequence ID" value="CAI49605"/>
        <property type="gene ID" value="NP_3028A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701632"/>
    <dbReference type="KEGG" id="nph:NP_3028A"/>
    <dbReference type="eggNOG" id="arCOG04511">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043006_0_0_2"/>
    <dbReference type="OrthoDB" id="156174at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2480">
        <property type="entry name" value="Periplasmic metal-binding protein Tp34-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055774">
        <property type="entry name" value="DUF7350"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038482">
        <property type="entry name" value="Tp34-type_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24041">
        <property type="entry name" value="DUF7350"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7350" evidence="2">
        <location>
            <begin position="291"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="234"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="406" mass="45085" checksum="0541DD9C0EAA93DE" modified="2017-05-10" version="1">MTRYTRRRLLGSSALAALGAFAGCTAEQPDDPEEDSPEEPDDGEPEDQPDAVPNIPQVDDPPNAVYKPTHREAMRHLPAVEAGDYRLSPMVTYPHSFWLITGEESEEVIPEQARGVHLMVTLWDAETGRVLPTDAGAEMRVLRDGNIVDQRAPWPMISQTMGFHFGDNVPLPADDTYTVEVDINPIQARRTGDFDGRFEEPATMSFEFEYDDDFREQVIGGVEYLDQDEWGQRGALAPPMEHGHDDHSDHDSHNDHNDHGSHNGHSDHNDHNGHNSHDGHQMPFSALPEADEYPGVDLGVHESGDARFVVRHLEASRFADDGYLLVSPRTPYNRVPLADMALSTEGPVETTLEQTLDSELGLHYGAAIDRPDSPFELVVESPPQVSRHAGYETAFLDMPSMTVGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX05</accession>
    <name>A0A1U7EX05_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Thioredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trxA5</name>
        <name evidence="1" type="ordered locus">NP_3114A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323270.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX05"/>
    <dbReference type="STRING" id="348780.NP_3114A"/>
    <dbReference type="EnsemblBacteria" id="CAI49648">
        <property type="protein sequence ID" value="CAI49648"/>
        <property type="gene ID" value="NP_3114A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703426"/>
    <dbReference type="KEGG" id="nph:NP_3114A"/>
    <dbReference type="eggNOG" id="arCOG04633">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135995_0_0_2"/>
    <dbReference type="OrthoDB" id="195058at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="124" mass="13723" checksum="4B412BFBFEB4CABA" modified="2017-05-10" version="1">MELETMRPNPAWDADAYAETVATFESLADDIVVKVWGGDWCKDCRSQLPDFGAALEAAGVDDIEHYPVEKADDGSKHGPKVDEYDIEYIPTVVVEDADSGTELARFIEEEPEPIAVYLAEQLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXE7</accession>
    <name>A0A1U7EXE7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3546A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323484.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXE7"/>
    <dbReference type="STRING" id="348780.NP_3546A"/>
    <dbReference type="EnsemblBacteria" id="CAI49864">
        <property type="protein sequence ID" value="CAI49864"/>
        <property type="gene ID" value="NP_3546A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701802"/>
    <dbReference type="KEGG" id="nph:NP_3546A"/>
    <dbReference type="eggNOG" id="arCOG11369">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2191100_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="108" mass="11183" checksum="CBFE4BAF640B7927" modified="2017-05-10" version="1">MDEKQSTTGTVVYDLKRRQHAVTTGIAGLLVGGSAAAAAGGGSIQQLTLAMFVAVFVGSGLYWLLRRGTAAVTGGSSRYHVRLPAATGDRHTEGDSMSDRSSAPMEGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXF7</accession>
    <name>A0A1U7EXF7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable oxidoreductase (Short-chain dehydrogenase family)</fullName>
            <ecNumber evidence="2">1.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3566A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323494.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXF7"/>
    <dbReference type="STRING" id="348780.NP_3566A"/>
    <dbReference type="EnsemblBacteria" id="CAI49874">
        <property type="protein sequence ID" value="CAI49874"/>
        <property type="gene ID" value="NP_3566A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701809"/>
    <dbReference type="KEGG" id="nph:NP_3566A"/>
    <dbReference type="eggNOG" id="arCOG01264">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_44_2_2"/>
    <dbReference type="OrthoDB" id="10454at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05327">
        <property type="entry name" value="retinol-DH_like_SDR_c_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004513">
        <property type="entry name" value="PRK05854.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004846">
        <property type="entry name" value="PRK06197.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157:SF31">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="313" mass="33364" checksum="21DE43036D93C96F" modified="2017-05-10" version="1">MASTWTADRMPDCTGTTVLITGANSGLGFEATKALAANGATVIMACRSTDRGEQAAAEIQDATAVGEAALDVRQCDLASLASVEAVAEDIAENYDALDVLCNNAGVMALPRQETDDGFEKQLGVNHLGHFALTGRLLDLLVESDGESRVVTHSSGAHEFGELDFDDLQREQSYGRWDAYGQSKLANLLFAYELQRRLTAADITDTISVACHPGYAATNLQYRGPKEDGSTLRMAMMKVANAVVGQSAQRGALPLLYASVAAGVGGGEYYGPGGLLNMRGAPERQSSNEASYDKRDARRLWEESESLTGVTYSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXH0</accession>
    <name>A0A1U7EXH0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3576A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXH0"/>
    <dbReference type="STRING" id="348780.NP_3576A"/>
    <dbReference type="EnsemblBacteria" id="CAI49879">
        <property type="protein sequence ID" value="CAI49879"/>
        <property type="gene ID" value="NP_3576A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3576A"/>
    <dbReference type="HOGENOM" id="CLU_2230454_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="105" mass="11673" checksum="5FABA2C27D72484A" modified="2017-05-10" version="1">MWMEIKPKHDSHSPLRPEIIPPPDSYEIAGLETDPGSFTTEYSEKQLRFNIMMVPKRTKRQADLRQRISEAPVGTSGYDIPPKDSGQPITAVNAWGVSTHGGGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXM1</accession>
    <name>A0A1U7EXM1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">XerC/D-like integrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">NP_3640A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323530.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXM1"/>
    <dbReference type="EnsemblBacteria" id="CAI49911">
        <property type="protein sequence ID" value="CAI49911"/>
        <property type="gene ID" value="NP_3640A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701832"/>
    <dbReference type="KEGG" id="nph:NP_3640A"/>
    <dbReference type="eggNOG" id="arCOG01241">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027562_15_0_2"/>
    <dbReference type="OrthoDB" id="142231at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00397">
        <property type="entry name" value="DNA_BRE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004107">
        <property type="entry name" value="Integrase_SAM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050090">
        <property type="entry name" value="Tyrosine_recombinase_XerCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30349">
        <property type="entry name" value="PHAGE INTEGRASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30349:SF92">
        <property type="entry name" value="SITE-SPECIFIC RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02899">
        <property type="entry name" value="Phage_int_SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core-binding (CB)" evidence="6">
        <location>
            <begin position="10"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="5">
        <location>
            <begin position="101"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="347" mass="39599" checksum="A00F43D4C0C3F2D8" modified="2017-05-10" version="1">MSSDNRTDESAAVDPVAYFLQDMTFHGRTERTRDAYERVLRDFEQFIETRRGTTLDAADRRACMAWVHSLRDEHAPSTVASYASYVHRFYAYMTQVGELESNPMALVTEEMDESIDTDPARRDVSVPEMRAFVTSLSHPLERALVGTLLKTGMRVGECCNLDVRDLHLDDDEVQAAFDVPHRGRLEGRPDSVYVSSEPARGEVYNGEERNASNKRKRDTVVPVDSELKRLLKAWLAVRPDTTSAAEPLFCSTSEWGDRATPSMVRSMVAEQARERGWYRQGGDSAENVTPHYFRHFFTTHLRDRTGDRGVVKYLRGDVASDVIDTYTHNWGDRVRTVYEANIYQLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXX9</accession>
    <name>A0A1U7EXX9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">IS1341-type transposase ISNph6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_3908A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323662.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXX9"/>
    <dbReference type="STRING" id="348780.NP_3908A"/>
    <dbReference type="EnsemblBacteria" id="CAI50045">
        <property type="protein sequence ID" value="CAI50045"/>
        <property type="gene ID" value="NP_3908A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701913"/>
    <dbReference type="KEGG" id="nph:NP_3908A"/>
    <dbReference type="eggNOG" id="arCOG00681">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050175_0_0_2"/>
    <dbReference type="OrthoDB" id="168528at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas12f1-like TNB" evidence="3">
        <location>
            <begin position="315"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="412"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="434" mass="50184" checksum="5AD6367F664D3446" modified="2017-05-10" version="1">MGEEATKTIQTRLHIASGERSWLHDARLASREIFNDTIRLTQRGYTRTEIQKEVDRDDFLRNNKCAVVGKALQTWDSYQSLKEWWENQDDPDGGEPTPPSTDKSGAYPLVMAHTEGYRLTVDDDTNRIQFRISPKPYKKVKGHLRGEPDVMEELRDALTSDELDVGQAELLYRDGVYYLHVTVTREFDVPEPDTADTLVGVDINERNVALTALDRETMRTKGTLVLDYGRVKQERQRYHTITKRCQEHGKTSIHHRLGDEEERFTEWVLHRLSRAVVEFAEQFSNPVIVFEDMSGIRDEIKYGTYMNRRLHKLPFHKFETFVSYKATWREIPTDTVDAYYNSQTCSCCGERGYRQGRRFRCTNDECDVVQDHADRNASVTIAWREKAKLDGNTTNYRTHKTQPQVRLVRLSGSGRVSRPTSSRSLAEQGVLAHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXZ0</accession>
    <name>A0A1U7EXZ0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ABC-type transport system permease protein (Probable substrate sulfate/thiosulfate/molybdate)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">abc13p</name>
        <name evidence="11" type="ordered locus">NP_3930A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323673.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXZ0"/>
    <dbReference type="STRING" id="348780.NP_3930A"/>
    <dbReference type="EnsemblBacteria" id="CAI50056">
        <property type="protein sequence ID" value="CAI50056"/>
        <property type="gene ID" value="NP_3930A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702409"/>
    <dbReference type="KEGG" id="nph:NP_3930A"/>
    <dbReference type="eggNOG" id="arCOG00164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_14_1_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
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    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
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        <location>
            <begin position="57"/>
            <end position="84"/>
        </location>
    </feature>
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        <location>
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            <end position="118"/>
        </location>
    </feature>
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            <begin position="138"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
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        </location>
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            <end position="257"/>
        </location>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009306"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI50056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="269" mass="27714" checksum="E7283B7C3581F574" modified="2017-05-10" version="1">MNDDDRSPGMLSGGLVVPGLLGGLLVVYFLLPFVAFLSRAGSVDVVAHLSSPAARRAVRNSLLTAPVATAIATGLGVPLAYLLARGSFRGKRLLEAAVVLPLVAPPVVGGVMLLTAVGQFTPLGRVAASLGVPLTDSLLGVVLAQLFVAAPFVILTARAGFAAIDERLEQASRSLGYGPIATFWRVSLPLASGAIVVGVTLTFVRAVGEFGATMMVANNPRTMPTQIWVDFVAGNIDGIVPLTLALLAVTLAVVFIVQRLGRTPAVVDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXZ4</accession>
    <name>A0A1U7EXZ4_NATPD</name>
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        <submittedName>
            <fullName evidence="2">Probable secreted glycoprotein</fullName>
        </submittedName>
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        <name evidence="2" type="ordered locus">NP_4012A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
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        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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        <property type="gene ID" value="NP_4012A"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="150" mass="15577" checksum="8C19B4256FD5EF20" modified="2017-05-10" version="1">MTDDRGVSVTVGYVLNLAIAAILVSALLMAGGSLIESQTEQVTHDELTVAGQQLAGDLASADRLARAGETETLTLQTDLPQRTAAGSYSITVAQPDGESAGHIELESTHPEVTVTVPLRSETPVENTTLSGGQVAIVYDDGEDVLEVQSS</sequence>
</entry>
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    <accession>A0A1U7EY07</accession>
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        <submittedName>
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    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <lineage>
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            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50094.1"/>
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    <dbReference type="SMR" id="A0A1U7EY07"/>
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    <dbReference type="EnsemblBacteria" id="CAI50094">
        <property type="protein sequence ID" value="CAI50094"/>
        <property type="gene ID" value="NP_4006A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701936"/>
    <dbReference type="KEGG" id="nph:NP_4006A"/>
    <dbReference type="eggNOG" id="arCOG02650">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037958_0_1_2"/>
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    <dbReference type="Proteomes" id="UP000002698">
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
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        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007516">
        <property type="entry name" value="Co_F420_Hydgase/DH_bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045220">
        <property type="entry name" value="FRHB/FDHB/HCAR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007525">
        <property type="entry name" value="FrhB_FdhB_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31332">
        <property type="entry name" value="7-HYDROXYMETHYL CHLOROPHYLL A REDUCTASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31332:SF0">
        <property type="entry name" value="7-HYDROXYMETHYL CHLOROPHYLL A REDUCTASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Fer4_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04432">
        <property type="entry name" value="FrhB_FdhB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04422">
        <property type="entry name" value="FrhB_FdhB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="36"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="67"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="379"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="401" mass="42419" checksum="D88CA8120BC09693" modified="2017-05-10" version="1">MSKRATPGVPSVGNDPRDSITESREPEEKTWFRDLDEAVIEADRCVQCGSCMAACPSDSIGIEPETGRPTLVRMCTGCSRCWDFCPRSGLRYERLNELEGESPDAAYAASADDDHEGQDGGAVTALLAGLLAAGEIDGAVVVSDGDDPLAGEATLATSPEELFESAGSTYSQTMQLGQVDDLVAESDLVDPDIALVGTPCVIQGAAALDRLQYDDELASVSLTVSLVCTRSFAADRLRSLLVGNGVDMDDVDSLEVVGGALTARDADGHRLLTKDVEAFDAAALRGCAECADAVGKAADISAGNVGSPDGKTTLLARTDDGTAAIEAAMEALDIDELDSTDALDAFADWNRRQAEATIPRERDSEASLTVTYTEHREAYDGTGREPEPLNPARVHQYEEWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY16</accession>
    <name>A0A1U7EY16_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable secreted glycoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4010A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323712.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY16"/>
    <dbReference type="STRING" id="348780.NP_4010A"/>
    <dbReference type="EnsemblBacteria" id="CAI50096">
        <property type="protein sequence ID" value="CAI50096"/>
        <property type="gene ID" value="NP_4010A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701938"/>
    <dbReference type="KEGG" id="nph:NP_4010A"/>
    <dbReference type="eggNOG" id="arCOG02911">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084673_1_0_2"/>
    <dbReference type="OrthoDB" id="118051at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055713">
        <property type="entry name" value="DUF7289"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23960">
        <property type="entry name" value="DUF7289"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="246" mass="27015" checksum="6EAB33885EADE0D6" modified="2017-05-10" version="1">MTDRAVSDVVGYVLVFSLIVATIGIVTTVGFATLDDRQSAEQLNNAERAFDVFANNADDIYRGGAPSRATEMRLAGGTLRYGEPTELVVRDADATAEDQNLTVRYTPLVYTDGDAEIAYEGGAIIRGDGHGGAMVREPPFRIHSERTLLPLLGTTRAPGPTAVSRDGTVQVETVRTSRVPTTPTDLQDADEIEIEVDSQRQSAWNRYLERQADTDSEWEYVADGVLRIETESLEAPQFRMRLRFAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY37</accession>
    <name>A0A1U7EY37_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4036A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323725.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY37"/>
    <dbReference type="STRING" id="348780.NP_4036A"/>
    <dbReference type="EnsemblBacteria" id="CAI50109">
        <property type="protein sequence ID" value="CAI50109"/>
        <property type="gene ID" value="NP_4036A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701949"/>
    <dbReference type="KEGG" id="nph:NP_4036A"/>
    <dbReference type="eggNOG" id="arCOG04635">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2230317_0_0_2"/>
    <dbReference type="OrthoDB" id="284158at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043867">
        <property type="entry name" value="DUF5827"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19145">
        <property type="entry name" value="DUF5827"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="87" mass="9994" checksum="00ADFE84494FCEDC" modified="2017-05-10" version="1">MPRPRSSFDDLRSFEFRDPEEVLDADKLYTVYEIARLLQGLDPDADLDPGTEGILLDWAIPWMLDHSDAFVFAEPEADEEPGYYGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY42</accession>
    <name>A0A1U7EY42_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metallophosphoesterase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4068A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323741.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY42"/>
    <dbReference type="STRING" id="348780.NP_4068A"/>
    <dbReference type="EnsemblBacteria" id="CAI50125">
        <property type="protein sequence ID" value="CAI50125"/>
        <property type="gene ID" value="NP_4068A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701958"/>
    <dbReference type="KEGG" id="nph:NP_4068A"/>
    <dbReference type="eggNOG" id="arCOG01154">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092313_3_1_2"/>
    <dbReference type="OrthoDB" id="113823at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07390">
        <property type="entry name" value="MPP_AQ1575"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="177" mass="20653" checksum="8C0D61DE10F6EC1F" modified="2017-05-10" version="1">MSRYLIADLHLDHAEVLEYTDRPFASVAEMNRRLVDGWNATVADSDEVVFVGDFAIPSEPTTIRRWVRRLAGDITFVVGDHDAGLHRSHGLDVRQEYRFSAGGYRFRCIHDPDDVDDRGTDWLIHGHHHDMRPDEYPFVTPDSRRINVSVELLDYEPLAVEELVDYLDRRQRLRKRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY49</accession>
    <name>A0A1U7EY49_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Endonuclease 3</fullName>
            <ecNumber evidence="10">4.2.99.18</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">nthB</name>
        <name evidence="10" type="ordered locus">NP_4064A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="4.2.99.18" evidence="10"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323739.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY49"/>
    <dbReference type="STRING" id="348780.NP_4064A"/>
    <dbReference type="EnsemblBacteria" id="CAI50123">
        <property type="protein sequence ID" value="CAI50123"/>
        <property type="gene ID" value="NP_4064A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701956"/>
    <dbReference type="KEGG" id="nph:NP_4064A"/>
    <dbReference type="eggNOG" id="arCOG00459">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012862_3_4_2"/>
    <dbReference type="OrthoDB" id="19248at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019104">
        <property type="term" value="F:DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003651">
        <property type="entry name" value="Endonuclease3_FeS-loop_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10359">
        <property type="entry name" value="A/G-SPECIFIC ADENINE GLYCOSYLASE/ENDONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10359:SF18">
        <property type="entry name" value="ENDONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10576">
        <property type="entry name" value="EndIII_4Fe-2S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001435">
        <property type="entry name" value="Nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="9">
        <location>
            <begin position="61"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI50123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="268" mass="28150" checksum="BB21055D4D2D63A8" modified="2017-05-10" version="1">MAEEPSVNISGSDAGGGAAADFEAATAATRAEAVVDELGDRYWQKAYGGRDGFECLVRTVLSQNTSDTASQPAHDALLERYGGGDLAAALADADQPTLAETISGAGLYNQKSTRLIALAEFVVETYGGADGFDGFVTEAPPDEVRETLLELNGVGPKTADCVLLFSGGRDGVFPVDTHVHRIARRMGLAPADADHEAVRSALEADVPGEKCGFGHTAMIQFGREYCTARKPACLDDPEACPLADRCDQVGVYPERGEVVDPADAPEIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY53</accession>
    <name>A0A1U7EY53_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF8101 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4084A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323749.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY53"/>
    <dbReference type="STRING" id="348780.NP_4084A"/>
    <dbReference type="EnsemblBacteria" id="CAI50133">
        <property type="protein sequence ID" value="CAI50133"/>
        <property type="gene ID" value="NP_4084A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701965"/>
    <dbReference type="KEGG" id="nph:NP_4084A"/>
    <dbReference type="eggNOG" id="arCOG06438">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_157004_0_0_2"/>
    <dbReference type="OrthoDB" id="239532at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058414">
        <property type="entry name" value="DUF8101"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26403">
        <property type="entry name" value="DUF8101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8101" evidence="1">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="87" mass="9477" checksum="87334D9C5E1F4655" modified="2017-05-10" version="1">MADDMPADVRASLSQLLSNARQSVRAGDTDTAASLLDTASTVAEHKLPDGDRRDRLRFGCSAARETLSDRELAAAYIEAMERRIPEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY61</accession>
    <name>A0A1U7EY61_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4118A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY61"/>
    <dbReference type="EnsemblBacteria" id="CAI50150">
        <property type="protein sequence ID" value="CAI50150"/>
        <property type="gene ID" value="NP_4118A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_4118A"/>
    <dbReference type="HOGENOM" id="CLU_3323066_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="38" mass="4192" checksum="8DB0DF2B4C562DD9" modified="2017-05-10" version="1">MVTDEATRTQYENNQPLGFAPTVTACWPGLLEPAEARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY99</accession>
    <name>A0A1U7EY99_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable secreted glycoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4216A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323815.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY99"/>
    <dbReference type="STRING" id="348780.NP_4216A"/>
    <dbReference type="EnsemblBacteria" id="CAI50199">
        <property type="protein sequence ID" value="CAI50199"/>
        <property type="gene ID" value="NP_4216A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702006"/>
    <dbReference type="KEGG" id="nph:NP_4216A"/>
    <dbReference type="eggNOG" id="arCOG06816">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_777592_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="356" mass="38066" checksum="2115CCD18E50AD24" modified="2017-05-10" version="1">MPSRRELLRLGGATLAAASAAGIAGCSEEAFEGPADEITGDDLALPGDRAVPAEDPDEEDPDEDTADETETEPTPDEDESELSDEEIATAFETAIEALSTNDEALADHDAAAAPEELDVAAVNERADTAETALEDIEPHVAGDDADDVEALQAVATYQRYVAGYETARTELLVRLDIIEAYVAIGEPENAAAELPSALDYHDEVEPELTAAERAAEHLQPVTRSETLGDGGHLDGERQTFERLGTTLEAADEFLDGAVSMREGLRAFDEERYEDALTAFSAASPSLAASERTFDQLETDEETPQRLRTNVIELRCTAGAFAEAATHFEAAASLATAGERWEAEDRFEDGEAALDQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYA1</accession>
    <name>A0A1U7EYA1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Family 3 CoA transferase</fullName>
            <ecNumber evidence="2">2.8.3.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4210A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.8.3.-" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50196.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939860.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYA1"/>
    <dbReference type="STRING" id="348780.NP_4210A"/>
    <dbReference type="EnsemblBacteria" id="CAI50196">
        <property type="protein sequence ID" value="CAI50196"/>
        <property type="gene ID" value="NP_4210A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702005"/>
    <dbReference type="KEGG" id="nph:NP_4210A"/>
    <dbReference type="eggNOG" id="arCOG02304">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033975_5_1_2"/>
    <dbReference type="OrthoDB" id="28444at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10540">
        <property type="entry name" value="Crotonobetainyl-coa:carnitine coa-transferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.10">
        <property type="entry name" value="formyl-coa transferase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050509">
        <property type="entry name" value="CoA-transferase_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003673">
        <property type="entry name" value="CoA-Trfase_fam_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044855">
        <property type="entry name" value="CoA-Trfase_III_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023606">
        <property type="entry name" value="CoA-Trfase_III_dom_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48228:SF5">
        <property type="entry name" value="ALPHA-METHYLACYL-COA RACEMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48228">
        <property type="entry name" value="SUCCINYL-COA--D-CITRAMALATE COA-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02515">
        <property type="entry name" value="CoA_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89796">
        <property type="entry name" value="CoA-transferase family III (CaiB/BaiF)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="331"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50196.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="391" mass="41683" checksum="D696AAF536C9BF78" modified="2017-05-10" version="1">MRLDGVRVLDLTRLLPGPYATQLLADAGADVVKVEDTDAGDYARHMPPLTDDGVGAVFDAVNRGKRSVAIDLKTDAGREAFYALVDDADVVIESFRPGVAERLGVDYGTLSERQPDLVYCSLTGYGQSGPYADRAGHDLNYVGIAGLLDMTRRGTDEAPRVPGYQVADMAGGLLAAFGIVSALLSRELGNTDGEYLDVALTDAVVSFSQALAPEALAGEDPRPGETPLAGAYPWYDVYETADGRYVTLGALEPQFWEAFCDAVDRPDLVEAHMTDDDAERAALRETLADIFGSKTREEWIAALDDVDAAVEAVYTPDEALEHPQIEARGYIDRPADGQPRVGFPLRGSAVPTGGAAEGDVPGHGEHTDTLLAAAGLSDEKRARLNDDGVVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYC1</accession>
    <name>A0A1U7EYC1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Propionyl-CoA carboxylase biotin carboxylase component</fullName>
            <ecNumber evidence="14">6.4.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">pccA1</name>
        <name evidence="14" type="ordered locus">NP_4252A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="6.4.1.3" evidence="14"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323833.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYC1"/>
    <dbReference type="STRING" id="348780.NP_4252A"/>
    <dbReference type="EnsemblBacteria" id="CAI50217">
        <property type="protein sequence ID" value="CAI50217"/>
        <property type="gene ID" value="NP_4252A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702467"/>
    <dbReference type="KEGG" id="nph:NP_4252A"/>
    <dbReference type="eggNOG" id="arCOG01591">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000395_3_1_2"/>
    <dbReference type="OrthoDB" id="31083at2157"/>
    <dbReference type="BRENDA" id="6.3.4.14">
        <property type="organism ID" value="3575"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004658">
        <property type="term" value="F:propionyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000003">
        <property type="entry name" value="Acetyl-CoA carboxylase biotin carboxyl carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000003">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000010">
        <property type="entry name" value="Propionyl-CoA carboxylase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050856">
        <property type="entry name" value="Biotin_carboxylase_complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006367">
        <property type="entry name" value="PRK08591.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18866">
        <property type="entry name" value="CARBOXYLASE:PYRUVATE/ACETYL-COA/PROPIONYL-COA CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18866:SF33">
        <property type="entry name" value="METHYLCROTONOYL-COA CARBOXYLASE SUBUNIT ALPHA, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0092">Biotin</keyword>
    <keyword evidence="4 14" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="13">
        <location>
            <begin position="1"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="12">
        <location>
            <begin position="121"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="11">
        <location>
            <begin position="507"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="490"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="497"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAI50217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="582" mass="62097" checksum="D20E742FC7444C5B" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYC3</accession>
    <name>A0A1U7EYC3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Acyl-CoA synthetase</fullName>
            <ecNumber evidence="7">6.2.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">acs2</name>
        <name evidence="7" type="ordered locus">NP_4242A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.-" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323828.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYC3"/>
    <dbReference type="STRING" id="348780.NP_4242A"/>
    <dbReference type="EnsemblBacteria" id="CAI50212">
        <property type="protein sequence ID" value="CAI50212"/>
        <property type="gene ID" value="NP_4242A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702485"/>
    <dbReference type="KEGG" id="nph:NP_4242A"/>
    <dbReference type="eggNOG" id="arCOG04201">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_10_2"/>
    <dbReference type="OrthoDB" id="193284at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016405">
        <property type="term" value="F:CoA-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015645">
        <property type="term" value="F:fatty acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004321">
        <property type="term" value="F:fatty-acyl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000005">
        <property type="entry name" value="Acyl-coenzyme A synthetase ACSM5, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051087">
        <property type="entry name" value="Mitochondrial_ACSM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43605:SF10">
        <property type="entry name" value="ACYL-COA SYNTHETASE MEDIUM CHAIN FAMILY MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43605">
        <property type="entry name" value="ACYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="5">
        <location>
            <begin position="46"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="6">
        <location>
            <begin position="464"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI50212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="560" mass="62413" checksum="0A93D8446DE38CA1" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYC5</accession>
    <name>A0A1U7EYC5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">IS1341-type transposase ISNph27</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">NP_4280A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323847.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYC5"/>
    <dbReference type="STRING" id="348780.NP_4280A"/>
    <dbReference type="DNASU" id="3702018"/>
    <dbReference type="EnsemblBacteria" id="CAI50231">
        <property type="protein sequence ID" value="CAI50231"/>
        <property type="gene ID" value="NP_4280A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702018"/>
    <dbReference type="KEGG" id="nph:NP_4280A"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040997_0_0_2"/>
    <dbReference type="OrthoDB" id="295419at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF26">
        <property type="entry name" value="TRANSPOSASE, PROBABLY IS605-TNPB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="7">
        <location>
            <begin position="194"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="8">
        <location>
            <begin position="328"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="409"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="409"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI50231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="450" mass="51799" checksum="9B277D931955201B" modified="2017-05-10" version="1">MKRANTFAMRPLTADDEQVLRDLLDASAALWNEINYQRLMRYNDKDGFEDEDVWDADTGALEGKYKAVLGASTAQTVRRANTEAWRSFFRLKKQFHDESNTSVTEHPEPPGFRGNKDDGRVLKGVVRKDAYSVEWGDRSRLEMVVGEQLRDRHNSPKSRLRLEIVGDPNWSDYEDQGRLELWYDETDCTFRASQPVTVSDDARATPLANEKAALDIGANNLVACTTTTGQQYLYEGRELFQRFRETTREIARLQSKLPEGRYSSKRIRRLYRKRTRRRDHAQETLCRDLLEQLYGEGVDTVYIGGLTDVLETHWSAETNAKTHNFWAFKQFTERLAWTADEYGISVEVRSEAWTSQECPQCGSTDRTTRQQDTLTCPCGFEGHADVTASETFLERHTEKAVRPMARPVRFEWDDHEWSESPRSPARESPKEQRTDPSTVPRDGNVVSGDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYD8</accession>
    <name>A0A1U7EYD8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Homolog to small CPxCG-related zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4284A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323849.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYD8"/>
    <dbReference type="EnsemblBacteria" id="CAI50233">
        <property type="protein sequence ID" value="CAI50233"/>
        <property type="gene ID" value="NP_4284A"/>
    </dbReference>
    <dbReference type="GeneID" id="54763325"/>
    <dbReference type="KEGG" id="nph:NP_4284A"/>
    <dbReference type="eggNOG" id="arCOG06252">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_200151_0_0_2"/>
    <dbReference type="OrthoDB" id="169264at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055998">
        <property type="entry name" value="DUF7576"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24461">
        <property type="entry name" value="DUF7576"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="58" mass="6018" checksum="1D1F7F4C901BE3BA" modified="2017-05-10" version="1">MVDPTSDLCENVAEADAPRCTACSEPIVADPGHRVVTEVVDSRVETAHFCGGCLSAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYF0</accession>
    <name>A0A1U7EYF0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Peptidase M42 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">NP_4306A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="8">Binds 2 divalent metal cations per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the peptidase M42 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323860.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYF0"/>
    <dbReference type="STRING" id="348780.NP_4306A"/>
    <dbReference type="EnsemblBacteria" id="CAI50244">
        <property type="protein sequence ID" value="CAI50244"/>
        <property type="gene ID" value="NP_4306A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702596"/>
    <dbReference type="KEGG" id="nph:NP_4306A"/>
    <dbReference type="eggNOG" id="arCOG01518">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047249_1_0_2"/>
    <dbReference type="OrthoDB" id="30642at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.40">
        <property type="entry name" value="Peptidase M42, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008007">
        <property type="entry name" value="Peptidase_M42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051464">
        <property type="entry name" value="Peptidase_M42_aminopept"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023367">
        <property type="entry name" value="Peptidase_M42_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32481">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32481:SF0">
        <property type="entry name" value="AMINOPEPTIDASE YPDE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05343">
        <property type="entry name" value="Peptidase_M42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001123">
        <property type="entry name" value="PepA_GA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101821">
        <property type="entry name" value="Aminopeptidase/glucanase lid domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="234"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="209"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001123-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001123-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI50244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="354" mass="37529" checksum="677F4611673AF46B" modified="2017-05-10" version="1">MDETRRAFLDELLSESTPSGFETAGQRRWLDYVSEFADETRVDDYGNAVAVYEGDPDAPSIAVAGHSDEIGLMVRDITDDGFLKLTRIGGSDRTVTRGKHVTVHADDGPVSGVIGQVAIHLRDTGSEEYDDINEQYVDIGVEDGDRAEELVDIGDPITLNTEIADLEDSRVAARGMDNRVGVWTAAEAFRRAVEQEPDATIYAVSTVQEEVGLKGAQMVGFDVAPDAAIAADVTHATDQPESPGDKGSGIELGSGPAVARGSTNHPKLVEAARTAADDADIDIQLAAAGSRTGTDADAFYISRGGIPSLNLGLPNRYMHTPVEVIDLDDLDAAADLLAAVAARADEFAPFSVDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYL9</accession>
    <name>A0A1U7EYL9_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Endonuclease III</fullName>
            <ecNumber evidence="13">4.2.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">DNA-(apurinic or apyrimidinic site) lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">nthA</name>
        <name evidence="13" type="synonym">nth</name>
        <name evidence="15" type="ordered locus">NP_4496A</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Hydrolyzes mismatched double-stranded DNA and polynucleotides, releasing free thymine.</text>
            <dbReference type="EC" id="3.2.2.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="13">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 13">Belongs to the Nth/MutY family.</text>
    </comment>
    <dbReference type="EC" id="4.2.99.18" evidence="13"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323954.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYL9"/>
    <dbReference type="STRING" id="348780.NP_4496A"/>
    <dbReference type="EnsemblBacteria" id="CAI50339">
        <property type="protein sequence ID" value="CAI50339"/>
        <property type="gene ID" value="NP_4496A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702080"/>
    <dbReference type="KEGG" id="nph:NP_4496A"/>
    <dbReference type="eggNOG" id="arCOG00459">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012862_3_4_2"/>
    <dbReference type="OrthoDB" id="84708at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0141016">
        <property type="term" value="F:G/T mismatch-specific thymine-DNA glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1670.10:FF:000001">
        <property type="entry name" value="Endonuclease III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.30:FF:000001">
        <property type="entry name" value="Endonuclease III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00942">
        <property type="entry name" value="Nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003651">
        <property type="entry name" value="Endonuclease3_FeS-loop_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000445">
        <property type="entry name" value="HhH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005759">
        <property type="entry name" value="Nth"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01083">
        <property type="entry name" value="nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10359">
        <property type="entry name" value="A/G-SPECIFIC ADENINE GLYCOSYLASE/ENDONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10359:SF18">
        <property type="entry name" value="ENDONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10576">
        <property type="entry name" value="EndIII_4Fe-2S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00633">
        <property type="entry name" value="HHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001435">
        <property type="entry name" value="Nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00525">
        <property type="entry name" value="FES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 13" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 13" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9 13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="15" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="11 13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 13" id="KW-0408">Iron</keyword>
    <keyword evidence="7 13" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="10 13 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0540">Nuclease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="14">
        <location>
            <begin position="44"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAI50339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="229" mass="25601" checksum="9E57AD6C375C613A" modified="2017-05-10" version="1">MGTPLEPRESQVAEVLDRLYEEYPEPEISLRFSNRLELLVAVVLSAQCTDERVNTVTETLFEKYETPEEYASADKEELASDIDSITYYNNKAGYLTSACADIVEKHNGEVPDTMSELTDLAGVGRKTANVVLQHGHEVVEGIVVDTHVQRISRRLGMTTEKRPDAIEDDLIDIVPQDDWKEFTHLLISHGRETCTARNPDCGDCILEDICPSSKLDNDIDLADGNGWSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYM8</accession>
    <name>A0A1U7EYM8_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribonuclease P protein component 4</fullName>
            <shortName evidence="8">RNase P component 4</shortName>
            <ecNumber evidence="8">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Rpp21</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 9" type="primary">rnp4</name>
        <name evidence="9" type="ordered locus">NP_4480A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Consists of a catalytic RNA component and at least 4-5 protein subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the eukaryotic/archaeal RNase P protein component 4 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="8"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323946.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYM8"/>
    <dbReference type="STRING" id="348780.NP_4480A"/>
    <dbReference type="EnsemblBacteria" id="CAI50331">
        <property type="protein sequence ID" value="CAI50331"/>
        <property type="gene ID" value="NP_4480A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703115"/>
    <dbReference type="KEGG" id="nph:NP_4480A"/>
    <dbReference type="eggNOG" id="arCOG04345">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079140_3_0_2"/>
    <dbReference type="OrthoDB" id="10058at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.420">
        <property type="entry name" value="Immunoglobulin FC, subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00757">
        <property type="entry name" value="RNase_P_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016432">
        <property type="entry name" value="RNP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007175">
        <property type="entry name" value="Rpr2/Snm1/Rpp21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14742:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14742">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04032">
        <property type="entry name" value="Rpr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004878">
        <property type="entry name" value="RNase_P_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6 8 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="7 8" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI50331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="92" mass="10828" checksum="C8D82ABF000F814C" modified="2017-05-10" version="1">MSVAEERLERLHELARDAASDGDDDLARRYVRRARRLAERNRLPLPRRFKRFTCDRCDAYLIPGRNARVRTRNGHLVITCDCGEHARYPYDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYN4</accession>
    <name>A0A1U7EYN4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="14">SSSF family transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">tp08</name>
        <name evidence="14" type="ordered locus">NP_4534A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323973.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYN4"/>
    <dbReference type="STRING" id="348780.NP_4534A"/>
    <dbReference type="EnsemblBacteria" id="CAI50358">
        <property type="protein sequence ID" value="CAI50358"/>
        <property type="gene ID" value="NP_4534A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703285"/>
    <dbReference type="KEGG" id="nph:NP_4534A"/>
    <dbReference type="eggNOG" id="arCOG01316">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
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            <end position="214"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="274"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="321"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="371"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="410"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="435"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="473"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAI50358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="510" mass="54492" checksum="453A78F08E2884A7" modified="2017-05-10" version="1">MTATLQVTIVVGYLLVALAIGLLAYRVTERAAEDFYLASRTFGTAVLLFTVFATLLSAFTFFGGPDNAYAFGPEWILVMGLMDGLIFAILWYLIGYKQWLLGQRHGYVTLGEMFGDRFGSTGVRALVAVVSLFWLFPYVMLQQIGAGGALAALTDGVVPFWAGASLITGFMILYVVLAGMRGIAWTDTLQGAFMLCMVWAALVWVLVAVDGGVSTINAGLTESSPEFFALGSDFYTPGTMLTFAISIAFGVAMFPQVNQRFFTAASETVLKRSFALWPLLVLLLFVPAFLLGTWAQGLGLQADVGAGESILPILLDTYTPAWFAALVIAGAIAAMMSSSDSMLLSGSSYFTRDVYRPLVDDGITERQEDRLGRIGVAVFAIAALAASVWAEAEAFGAVAVGSVLVEIGDLAFGGFAQLTVPVLVALYWRGTTKQGLVAGILLPQAVYLAFNFLPATEVGSAMLFSETYLGWGISIYCLLLGLVATVVVSAATTKADSERPALFFESLRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYN8</accession>
    <name>A0A1U7EYN8_NATPD</name>
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        <submittedName>
            <fullName evidence="4">CBS domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cbs1</name>
        <name evidence="4" type="ordered locus">NP_4504A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="STRING" id="348780.NP_4504A"/>
    <dbReference type="EnsemblBacteria" id="CAI50343">
        <property type="protein sequence ID" value="CAI50343"/>
        <property type="gene ID" value="NP_4504A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702579"/>
    <dbReference type="KEGG" id="nph:NP_4504A"/>
    <dbReference type="eggNOG" id="arCOG00601">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035203_0_0_2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd17780">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CBS_domain-containing"/>
    </dbReference>
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        <property type="entry name" value="RHF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014651">
        <property type="entry name" value="UCP036983_2CBS_MJ1404"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48108">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48108:SF35">
        <property type="entry name" value="ZINC METALLOPROTEASE MJ0392"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
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        <property type="match status" value="4"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Ribosome binding protein Y (YfiA homologue)"/>
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    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="7"/>
            <end position="62"/>
        </location>
    </feature>
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        <location>
            <begin position="66"/>
            <end position="122"/>
        </location>
    </feature>
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        <location>
            <begin position="129"/>
            <end position="185"/>
        </location>
    </feature>
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        <location>
            <begin position="207"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="381" mass="42723" checksum="0DDA6CFFDFE429F8" modified="2017-05-10" version="1">MNIADIATKDYAQVDADERLGKVRSVFEQDNPKGIVVTNDGDYEGIITERQLLQSHVEDDAKANVLTRSNVPQVNREDGVREVARMLVEGDTKVAPVFEHGDLWGVITDNAILNAVLDNLDVLTVDQIHTEEVVTLREDDTLGKAINHLRENAISRLPVVNDNGKLTGIITTHDIADFAVRKMDRQRTGDRRGDTERMLDLPVYDAMNSPVQTISAEASVRDAVERMFENDFGGLVVTPDDNDEFVAGILTKTDVLRALTYTEEESMDVQITNIGLLDGISREAVRESITEVADKYKDMQVHHAHVRFTEHKERMRGTPLIYCQIRLRTNRGQIAGSGEGYGSKTAFRVAVDKLERNVLELKGIRADEEYKGQLLRKLNEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYZ9</accession>
    <name>A0A1U7EYZ9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">YdjM family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4794A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324100.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYZ9"/>
    <dbReference type="STRING" id="348780.NP_4794A"/>
    <dbReference type="EnsemblBacteria" id="CAI50488">
        <property type="protein sequence ID" value="CAI50488"/>
        <property type="gene ID" value="NP_4794A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702178"/>
    <dbReference type="KEGG" id="nph:NP_4794A"/>
    <dbReference type="eggNOG" id="arCOG04605">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081182_0_0_2"/>
    <dbReference type="OrthoDB" id="252570at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="214" mass="22399" checksum="88869A6D5E41A80D" modified="2017-05-10" version="1">MPPTLVNIALGVLVGVALLGAAFDRRSLTVVAAAAAAPDADAALELVAIGTTNAVFHSLFIPLAAAVLLSYDTRLRETSWLRSWYGWYGVRVAWVATAAYAVAGIAPELFSPAGAAVLYPLSDRYFAVVGRLVISTQEGLVQTYVEFGSGAFGLASPGTTDTHHVRSWVAPGDDDRRLYLVESGWQAVLVGGAVVAAVAKSRVERIDRAAGGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ05</accession>
    <name>A0A1U7EZ05_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phytoene synthase</fullName>
            <ecNumber evidence="4">2.5.1.32</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">crtB</name>
        <name evidence="4" type="ordered locus">NP_4770A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carotenoid biosynthesis; phytoene biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.32" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324088.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ05"/>
    <dbReference type="STRING" id="348780.NP_4770A"/>
    <dbReference type="EnsemblBacteria" id="CAI50476">
        <property type="protein sequence ID" value="CAI50476"/>
        <property type="gene ID" value="NP_4770A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702632"/>
    <dbReference type="KEGG" id="nph:NP_4770A"/>
    <dbReference type="eggNOG" id="arCOG02936">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037269_1_2_2"/>
    <dbReference type="OrthoDB" id="305023at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004311">
        <property type="term" value="F:geranylgeranyl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051996">
        <property type="term" value="F:squalene synthase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016117">
        <property type="term" value="P:carotenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00683">
        <property type="entry name" value="Trans_IPPS_HH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000020">
        <property type="entry name" value="Phytoene synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002060">
        <property type="entry name" value="Squ/phyt_synthse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019845">
        <property type="entry name" value="Squalene/phytoene_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044843">
        <property type="entry name" value="Trans_IPPS_bact-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033904">
        <property type="entry name" value="Trans_IPPS_HH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31480">
        <property type="entry name" value="BIFUNCTIONAL LYCOPENE CYCLASE/PHYTOENE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00494">
        <property type="entry name" value="SQS_PSY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01212">
        <property type="entry name" value="Phytoene_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01018">
        <property type="entry name" value="Squalene/Phytoene_Synthase_Lik"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01044">
        <property type="entry name" value="SQUALEN_PHYTOEN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01045">
        <property type="entry name" value="SQUALEN_PHYTOEN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0125">Carotenoid biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="318" mass="36320" checksum="3686C92F20570D22" modified="2017-05-10" version="1">MVDSTQLAESKSIHRETGKTFYYATRLLPERVRHPTYVLYAFFRLADEVVDDPGALSPDEQRQELLDIREAALGRTEPDDPVLEAFSEVRRRHDIPDEEVEVFIDAMLTDIEKSRYETYEELEAYMQGSASAVGVMMTMLMGTEDTDAAVPRARALGEAFQLTNFLRDIGEDIEERNRIYLPQETLDAYDVDPADIEAHRFTDELGDAIEHELERAERRYREGVAGIKYLPVDCQLPVLTAAVLYADHHRLIRDHDLDTVTQTPSLGTLRKLALVARTRWHWAWTKDPEAVFSRVSPISMGEETAIPSERPRDPLPMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ29</accession>
    <name>A0A1U7EZ29_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Transcription initiation factor IIB</fullName>
            <shortName evidence="10">TFIIB</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">tfbA6</name>
        <name evidence="10" type="synonym">tfb</name>
        <name evidence="13" type="ordered locus">NP_4822A</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre-initiation complex (DNA-TBP-TFIIB).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the TFIIB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50502.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324114.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ29"/>
    <dbReference type="STRING" id="348780.NP_4822A"/>
    <dbReference type="EnsemblBacteria" id="CAI50502">
        <property type="protein sequence ID" value="CAI50502"/>
        <property type="gene ID" value="NP_4822A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703238"/>
    <dbReference type="KEGG" id="nph:NP_4822A"/>
    <dbReference type="eggNOG" id="arCOG01981">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043736_0_1_2"/>
    <dbReference type="OrthoDB" id="7429at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20549">
        <property type="entry name" value="CYCLIN_TFIIB_archaea_like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20550">
        <property type="entry name" value="CYCLIN_TFIIB_archaea_like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000023">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.170:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00383">
        <property type="entry name" value="TF2B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023484">
        <property type="entry name" value="TFIIB_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023486">
        <property type="entry name" value="TFIIB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001658">
        <property type="entry name" value="PRK00423.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF13">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00782">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="1" evidence="10">
        <location>
            <begin position="149"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="12">
        <location>
            <begin position="149"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="repeat" description="2" evidence="10">
        <location>
            <begin position="243"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="12">
        <location>
            <begin position="243"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="14"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00053882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAI50502.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="329" mass="36643" checksum="A8DE5100B0788AF2" modified="2017-05-10" version="1">MVATVTSGSTNQRTSREASTEAEKAEEAAATAETVEPDAVDPDDVVKTADGELIHEPTGLVLEDEHIDRGPEWRAFTHSERQSKSRVGAPTTQTMHDKGLTTQIDWKDKDAYGQSISSDKRSQMNRLRKWQERIRTKDAGERNLQFALSEIDRMASALGVPRSVREVASVTYRRALDEDLIRGRSIEGAATATLYAACRQESIPRSLDEVAEVARVEQKEIGRTYRYLSQELGLEIKPADPKEYVPRFCSELALSEAVEQKTREIIDVTAEEGMLSGKSPTGYAAAAIYAASLLCNEKKTQREVAEVAQVTEVTIRNRYQEQIEALELH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ68</accession>
    <name>A0A1U7EZ68_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Probable anaerobic dehydrogenase membrane anchor subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">NP_4944A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NrfD family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324175.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ68"/>
    <dbReference type="STRING" id="348780.NP_4944A"/>
    <dbReference type="EnsemblBacteria" id="CAI50563">
        <property type="protein sequence ID" value="CAI50563"/>
        <property type="gene ID" value="NP_4944A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702699"/>
    <dbReference type="KEGG" id="nph:NP_4944A"/>
    <dbReference type="eggNOG" id="arCOG02025">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021295_1_0_2"/>
    <dbReference type="OrthoDB" id="200631at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN NRFD"/>
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    </dbReference>
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        <property type="entry name" value="NrfD"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="22"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="64"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="247"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="286"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="326"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="358"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="402"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008929"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI50563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="446" mass="49389" checksum="8911B1F750AB590B" modified="2017-05-10" version="1">MSSKTPSTDDIVRPMQTTGKKFYIVFALAAVVGLAWIGLWLYQVDRGLAVTNLSDWGSGGGVTWGLYIGAFIWWVGIAHGGIILSAAVRLFGMKKYQPVARLAELLTLGALSMAGFYIVVHMGRPDRMVTSIVDSYPVTVHQSPLVWDVTVITLYMVMTATYLALTLRYDMTRLRDDLPNKLEPLYKVMTIGYTKKEDQIIERMVWWLALGIIVMAPLLLHGGVIPWLFALVPSMPGWDGAVQGPQFLSIALTSALAGVIILSTVLRRGYDWYHILTDKVVQGLTLWLGLFSLLFLWLQLQQIITGVYAAPVDEAYVTMAKITSPVYWFAIGLVIIALAYIFAQAVKPEKYFTATRSIIASVMVLVGTLVEKVFFVVEGLQYPTFGMYYSVPGDYFPSPIELASVAGTIALCTLFFMAVLKLFPVIELHAIEHLRGDGHGHSEADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZF3</accession>
    <name>A0A1U7EZF3_NATPD</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">NP_5214A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
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            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAI50698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZF3"/>
    <dbReference type="STRING" id="348780.NP_5214A"/>
    <dbReference type="EnsemblBacteria" id="CAI50698">
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        <property type="gene ID" value="NP_5214A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702298"/>
    <dbReference type="KEGG" id="nph:NP_5214A"/>
    <dbReference type="eggNOG" id="arCOG02945">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010776_0_0_2"/>
    <dbReference type="OrthoDB" id="124691at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055710">
        <property type="entry name" value="DUF7286"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23957">
        <property type="entry name" value="DUF7286"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="59"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="385"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="942"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="954"/>
            <end position="963"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="963" mass="101112" checksum="155CFFBED104015D" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZG7</accession>
    <name>A0A1U7EZG7_NATPD</name>
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        </submittedName>
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        <name evidence="1" type="primary">argW</name>
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    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
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            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZG7"/>
    <dbReference type="STRING" id="348780.NP_5256A"/>
    <dbReference type="EnsemblBacteria" id="CAI50719">
        <property type="protein sequence ID" value="CAI50719"/>
        <property type="gene ID" value="NP_5256A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702312"/>
    <dbReference type="KEGG" id="nph:NP_5256A"/>
    <dbReference type="eggNOG" id="arCOG01588">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_195720_0_0_2"/>
    <dbReference type="OrthoDB" id="159847at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="LysW"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01206">
        <property type="entry name" value="lysW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40393:SF1">
        <property type="entry name" value="LYSINE BIOSYNTHESIS PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40393">
        <property type="entry name" value="LYSINE BIOSYNTHESIS PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21344">
        <property type="entry name" value="Zn_ribbon_LysW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="54" mass="5888" checksum="1F4218381808D28C" modified="2017-05-10" version="1">MAECPECGAELDLHDDLETGEIVDCSTCGAELEVINHDPVELDTAPELEEDWGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZI0</accession>
    <name>A0A1U7EZI0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5210A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50696.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZI0"/>
    <dbReference type="STRING" id="348780.NP_5210A"/>
    <dbReference type="EnsemblBacteria" id="CAI50696">
        <property type="protein sequence ID" value="CAI50696"/>
        <property type="gene ID" value="NP_5210A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702296"/>
    <dbReference type="KEGG" id="nph:NP_5210A"/>
    <dbReference type="eggNOG" id="arCOG05166">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135432_0_0_2"/>
    <dbReference type="OrthoDB" id="308110at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055709">
        <property type="entry name" value="DUF7285"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23956">
        <property type="entry name" value="DUF7285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50696.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="132" mass="13671" checksum="5E2C4F72F1BF0B27" modified="2017-05-10" version="1">MWSSRDSGQTEPLAALVAVFALGVGLTLYAGALDSVWPLLSGTHETAALAVDATASEAADFGVVAPPITEAAAAASPAGYRLNATLRADGRMWHAGQPRPADADCDRRTVSVRTAPGRVSRGLLEVCVWRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZJ2</accession>
    <name>A0A1U7EZJ2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HAD superfamily hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5310A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324355.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZJ2"/>
    <dbReference type="STRING" id="348780.NP_5310A"/>
    <dbReference type="EnsemblBacteria" id="CAI50746">
        <property type="protein sequence ID" value="CAI50746"/>
        <property type="gene ID" value="NP_5310A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702327"/>
    <dbReference type="KEGG" id="nph:NP_5310A"/>
    <dbReference type="eggNOG" id="arCOG02291">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100099_0_0_2"/>
    <dbReference type="OrthoDB" id="238326at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051540">
        <property type="entry name" value="S-2-haloacid_dehalogenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43316:SF3">
        <property type="entry name" value="HALOACID DEHALOGENASE, TYPE II (AFU_ORTHOLOGUE AFUA_2G07750)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43316">
        <property type="entry name" value="HYDROLASE, HALOACID DELAHOGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="206" mass="21063" checksum="66F91B403C04E101" modified="2017-05-10" version="1">MAVAFDLFGTLVDVDTPADPAEAVAAELEARGVAVPSAFGAAYREQHTDAPEGAEVPLPAHVAAVLAAHDVEVTNNAARRAVVAAFDPDVRVRPGVSAALAAASERGPVGLCSNCSVPELVGRTLVRADLRGTFDAVVTSVGCGWRKPHPNSFGELADALGVAVEDIVFVGDDPDTDGGIEAVGGRFVDVSDTPLSALPEQLDADE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZN7</accession>
    <name>A0A1U7EZN7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Small CPxCG-related zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3853C</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CCI69584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZN7"/>
    <dbReference type="STRING" id="348780.NP_3853C"/>
    <dbReference type="EnsemblBacteria" id="CCI69584">
        <property type="protein sequence ID" value="CCI69584"/>
        <property type="gene ID" value="NP_3853C"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3853C"/>
    <dbReference type="HOGENOM" id="CLU_202925_2_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.20.30">
        <property type="entry name" value="reverse gyrase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026870">
        <property type="entry name" value="Zinc_ribbon_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13240">
        <property type="entry name" value="Zn_Ribbon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zinc-ribbon" evidence="1">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCI69584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="50" mass="5652" checksum="05887480CE001967" modified="2017-05-10" version="1">MSSLQRLKRVFTLTDRTVSVVECRQCGTSLTDETETCPECGSQEIACYEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILS0</accession>
    <name>Q3ILS0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3246A</name>
        <name evidence="3" type="ordered locus">NP_7070A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>PL23</name>
    </geneLocation>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
            <strain evidence="3">Gabara</strain>
            <plasmid evidence="4">PL23</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49714.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CR936259">
        <property type="protein sequence ID" value="CAI50951.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323336.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILS0"/>
    <dbReference type="STRING" id="348780.NP_3246A"/>
    <dbReference type="EnsemblBacteria" id="CAI49714">
        <property type="protein sequence ID" value="CAI49714"/>
        <property type="gene ID" value="NP_3246A"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI50951">
        <property type="protein sequence ID" value="CAI50951"/>
        <property type="gene ID" value="NP_7070A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703494"/>
    <dbReference type="KEGG" id="nph:NP_3246A"/>
    <dbReference type="KEGG" id="nph:NP_7070A"/>
    <dbReference type="HOGENOM" id="CLU_2875181_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49714.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50951.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="63" mass="6382" checksum="D05474959E248C1C" modified="2005-11-08" version="1">MDDAVPIVALAVAGASGVTWAASQLWFGALGEMVAGLGIVFGLIVTVALIAQERAARPTEVDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILT9</accession>
    <name>Q3ILT9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3306A</name>
        <name evidence="3" type="ordered locus">NP_7030A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>PL23</name>
    </geneLocation>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
            <strain evidence="3">Gabara</strain>
            <plasmid evidence="3">PL23</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CR936259">
        <property type="protein sequence ID" value="CAI50931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323365.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILT9"/>
    <dbReference type="STRING" id="348780.NP_3306A"/>
    <dbReference type="EnsemblBacteria" id="CAI49744">
        <property type="protein sequence ID" value="CAI49744"/>
        <property type="gene ID" value="NP_3306A"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI50931">
        <property type="protein sequence ID" value="CAI50931"/>
        <property type="gene ID" value="NP_7030A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703475"/>
    <dbReference type="KEGG" id="nph:NP_3306A"/>
    <dbReference type="KEGG" id="nph:NP_7030A"/>
    <dbReference type="HOGENOM" id="CLU_2505049_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="85" mass="9480" checksum="886D5D921833783E" modified="2005-11-08" version="1">MSAWLRFGYGLMATVGLAVGGLIYQQVFVAELLPIAPTEGPFATPVIWLDRLVPVILVGLLLFVWAWVIAGSVQEERTLDRRRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILU4</accession>
    <name>Q3ILU4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3296A</name>
        <name evidence="3" type="ordered locus">NP_7020A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>PL23</name>
    </geneLocation>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
            <strain evidence="3">Gabara</strain>
            <plasmid evidence="3">PL23</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CR936259">
        <property type="protein sequence ID" value="CAI50926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323360.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILU4"/>
    <dbReference type="STRING" id="348780.NP_3296A"/>
    <dbReference type="EnsemblBacteria" id="CAI49739">
        <property type="protein sequence ID" value="CAI49739"/>
        <property type="gene ID" value="NP_3296A"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI50926">
        <property type="protein sequence ID" value="CAI50926"/>
        <property type="gene ID" value="NP_7020A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703470"/>
    <dbReference type="KEGG" id="nph:NP_3296A"/>
    <dbReference type="KEGG" id="nph:NP_7020A"/>
    <dbReference type="HOGENOM" id="CLU_2217131_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="106" mass="11551" checksum="400E8B285C6CFD31" modified="2005-11-08" version="1">MYQRYYFALAVGATLLTAAWLFRPDERTTITSFGAFVSWSLTALVGGEVERVTSDGSVVAAPVPAEVRWLTFLFALLSLLALILYTVGVYPPNTDDEPLSDSQTNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM10</accession>
    <name>Q3IM10_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">XerC/D-like integrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">NP_6156A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7 8">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324457.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM10"/>
    <dbReference type="EnsemblBacteria" id="CAI50858">
        <property type="protein sequence ID" value="CAI50858"/>
        <property type="gene ID" value="NP_6156A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694630"/>
    <dbReference type="KEGG" id="nph:NP_6156A"/>
    <dbReference type="HOGENOM" id="CLU_062981_0_0_2"/>
    <dbReference type="OrthoDB" id="194919at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025269">
        <property type="entry name" value="SAM-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050090">
        <property type="entry name" value="Tyrosine_recombinase_XerCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30349:SF41">
        <property type="entry name" value="INTEGRASE_RECOMBINASE PROTEIN MJ0367-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30349">
        <property type="entry name" value="PHAGE INTEGRASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13102">
        <property type="entry name" value="Phage_int_SAM_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core-binding (CB)" evidence="6">
        <location>
            <begin position="9"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI50858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="412" mass="47037" checksum="8DB3A9EE64012A55" modified="2005-11-08" version="1">MTPRERADQSLESSLERYLQDKGKGRGGDGGNYRRNVERELERFVEWAAGEHGRDDWTGITPDGVGREPTFDDIDERTFREYARHLAGDRGLKQNTVKTYYRYISAWCGWCVNEGYLEAHYAQRASAMAPLPDDDGRKPGDQQAWTSDQRHTLTRHVDEQAREAIETYTTLPEETAPIDKQRARYAALKAARDRALVFVLAYTAVRVGELLRDPDDPRRRGVRWEDIDLKDGRMDVYRKKQQWDAASLPDPTISPLQSYRKLLAPPTERWPVFPTFDQRTLAELVKETLAERGNRPDDIEAQRDGYARDLLLALDADMRPPSMTTDGGRSVLQRLSDDAEIDIDHPKHDYLAPHGGRRGMGEVLVRAFGYTVAARYLDNSEEMVRKRYSHIEAGELGDVAAEALDEIDGSRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM26</accession>
    <name>Q3IM26_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC-type transport system permease protein (Probable substrate phosphate/phosphonate)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">phnE3</name>
        <name evidence="9" type="synonym">abc18p2</name>
        <name evidence="9" type="ordered locus">NP_6120A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9 10">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324441.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM26"/>
    <dbReference type="EnsemblBacteria" id="CAI50840">
        <property type="protein sequence ID" value="CAI50840"/>
        <property type="gene ID" value="NP_6120A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694677"/>
    <dbReference type="KEGG" id="nph:NP_6120A"/>
    <dbReference type="HOGENOM" id="CLU_064254_1_1_2"/>
    <dbReference type="OrthoDB" id="338493at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015416">
        <property type="term" value="F:ABC-type phosphonate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005769">
        <property type="entry name" value="PhnE/PtxC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01097">
        <property type="entry name" value="PhnE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30043:SF1">
        <property type="entry name" value="ABC TRANSPORT SYSTEM PERMEASE PROTEIN P69"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30043">
        <property type="entry name" value="PHOSPHONATES TRANSPORT SYSTEM PERMEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="16"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="238"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="72"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI50840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="263" mass="28616" checksum="10387F751220F546" modified="2005-11-08" version="1">MSEPPHWEYRTRRQRLARFVGLLLTLVVFAWAWQTLEVRYDFVRSAPAEVEDLLVRMYPPDAAYSYEIVGPMIETVNIAVLGTGLSVVLSVPIAYLAAENTSPNRLTYLLGKFVVTATRSVSVIIWALLFVVIFGPGPLAGVVAIAVRSIGFIGKLLAEAIEEIQPRQPEAIRATGGNAVDVMVYGIVPQITPAVVGITTHRWDINVRSSTVLGFVGAGGIGTELFAVLDFFRWDAALTILIAILVVVLVSEGISAVIRGKIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM42</accession>
    <name>Q3IM42_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_6086A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324425.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM42"/>
    <dbReference type="EnsemblBacteria" id="CAI50823">
        <property type="protein sequence ID" value="CAI50823"/>
        <property type="gene ID" value="NP_6086A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694611"/>
    <dbReference type="KEGG" id="nph:NP_6086A"/>
    <dbReference type="HOGENOM" id="CLU_1010541_0_0_2"/>
    <dbReference type="OrthoDB" id="270566at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="275" mass="29350" checksum="10B045EF6A82CE36" modified="2005-11-08" version="1">MRRRALLAATGTACGVLAGCLDIDSRFSGSPADARESLDLAVPEIQLRTSYLYPTHPDAAAVRAGGSDQFIFLDVATESTVSYQDISLQLEGEAETPVERVGRETMDSVFHHGWMADSALPARPYEGGDGLVGFSVPRDIAPVESATLVAETKEKRGEWSAPAETVAQLHTPPEFEASLSLPEEIVWESTFEAELSVTNDGGRAGVFVAVLGVESAMHPNRITTTVPAGETETWTTQLEYPSPFEGGSDEPRASVTYELDSGEEVVTATTTVVAD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-05-20" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q3INN3</accession>
    <name>SUCC_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Succinate--CoA ligase [ADP-forming] subunit beta</fullName>
            <ecNumber evidence="1">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Succinyl-CoA synthetase subunit beta</fullName>
            <shortName evidence="1">SCS-beta</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">sucC</name>
        <name type="ordered locus">NP_4356A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>succinate + ATP + CoA = succinyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="1">
            <dbReference type="Rhea" id="RHEA:17663"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP + succinate + CoA = succinyl-CoA + GDP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:22120"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="1">
            <dbReference type="Rhea" id="RHEA:22122"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the succinate/malate CoA ligase beta subunit family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323885.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3INN3"/>
    <dbReference type="SMR" id="Q3INN3"/>
    <dbReference type="STRING" id="348780.NP_4356A"/>
    <dbReference type="EnsemblBacteria" id="CAI50269">
        <property type="protein sequence ID" value="CAI50269"/>
        <property type="gene ID" value="NP_4356A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703219"/>
    <dbReference type="KEGG" id="nph:NP_4356A"/>
    <dbReference type="eggNOG" id="arCOG01337">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037430_0_2_2"/>
    <dbReference type="OrthoDB" id="146449at2157"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042709">
        <property type="term" value="C:succinate-CoA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004776">
        <property type="term" value="F:succinate-CoA ligase (GDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006104">
        <property type="term" value="P:succinyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000002">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.261:FF:000007">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00558">
        <property type="entry name" value="Succ_CoA_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013650">
        <property type="entry name" value="ATP-grasp_succ-CoA_synth-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017866">
        <property type="entry name" value="Succ-CoA_synthase_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005809">
        <property type="entry name" value="Succ_CoA_ligase-like_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001913">
        <property type="entry name" value="PRK00696.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01016">
        <property type="entry name" value="sucCoAbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815:SF10">
        <property type="entry name" value="SUCCINATE--COA LIGASE [GDP-FORMING] SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815">
        <property type="entry name" value="SUCCINYL-COA SYNTHETASE BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08442">
        <property type="entry name" value="ATP-grasp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001554">
        <property type="entry name" value="SucCS_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01217">
        <property type="entry name" value="SUCCINYL_COA_LIG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="chain" id="PRO_1000082136" description="Succinate--CoA ligase [ADP-forming] subunit beta">
        <location>
            <begin position="1"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="1">
        <location>
            <begin position="9"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="52"/>
            <end position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit alpha</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="313"/>
            <end position="315"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit alpha</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00558"/>
        </source>
    </evidence>
    <sequence length="380" mass="40710" checksum="A2471A4AE855DB0A" modified="2005-11-08" version="1">MKLHEYQAKGVFADAGIPVPESTLASTVDEAVEAADDIGYPVAIKAQVQVGGRGKAGGIKLVENADEAREAADEILGMDLKGLHVDRVLVEAAVDFTDELYVGVTMDRAAGKPVAMVSTRGGVNIEEVAEEDPDAIVREHIDPAFGMHPFQARKAAFEAGVDRAVANDVASILTQLYELWADKDATEIEVNPLMVTDDDEVIAADAVMNIDEDALFRHPDLEEMEEEAAGDELEAKANEYGFDYVRLSGNTGIIGNGAGLVMTTLDLVDHYGGEPANFLDIGGGAKAERVANALDMVFSDDNVDSVVFNIFGGITRGDEVAKGINEALEQFDEIPKPVVVRLAGTNAEEGMEILNTDLVTVEKTLEDAVQRAVKYAEEEQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-30" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IPL2</accession>
    <name>SYE_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamate--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glutamyl-tRNA synthetase</fullName>
            <shortName evidence="1">GluRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gltX</name>
        <name type="ordered locus">NP_3694A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Glu) + L-glutamate + ATP = L-glutamyl-tRNA(Glu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.17" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323556.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IPL2"/>
    <dbReference type="SMR" id="Q3IPL2"/>
    <dbReference type="STRING" id="348780.NP_3694A"/>
    <dbReference type="EnsemblBacteria" id="CAI49938">
        <property type="protein sequence ID" value="CAI49938"/>
        <property type="gene ID" value="NP_3694A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702761"/>
    <dbReference type="KEGG" id="nph:NP_3694A"/>
    <dbReference type="eggNOG" id="arCOG04302">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001882_1_3_2"/>
    <dbReference type="OrthoDB" id="10470at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004818">
        <property type="term" value="F:glutamate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043604">
        <property type="term" value="P:amide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="entry name" value="Ribosomal Protein L25, Chain P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02076">
        <property type="entry name" value="Glu_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050132">
        <property type="entry name" value="Gln/Glu-tRNA_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004526">
        <property type="entry name" value="Glu-tRNA-synth_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020059">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_codon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020056">
        <property type="entry name" value="Rbsml_bL25/Gln-tRNA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011035">
        <property type="entry name" value="Ribosomal_bL25/Gln-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049437">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00463">
        <property type="entry name" value="gltX_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003169">
        <property type="entry name" value="PRK04156.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097:SF5">
        <property type="entry name" value="GLUTAMATE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097">
        <property type="entry name" value="GLUTAMINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03950">
        <property type="entry name" value="tRNA-synt_1c_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20974">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50715">
        <property type="entry name" value="Ribosomal protein L25-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000237425" description="Glutamate--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="431"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="1">
        <location>
            <begin position="114"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="571" mass="64418" checksum="80D88F8AB47E9D12" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IPM8</accession>
    <name>RS28_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein eS28</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">30S ribosomal protein S28e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps28e</name>
        <name type="ordered locus">NP_3662A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS28 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323540.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IPM8"/>
    <dbReference type="SMR" id="Q3IPM8"/>
    <dbReference type="STRING" id="348780.NP_3662A"/>
    <dbReference type="EnsemblBacteria" id="CAI49922">
        <property type="protein sequence ID" value="CAI49922"/>
        <property type="gene ID" value="NP_3662A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703179"/>
    <dbReference type="KEGG" id="nph:NP_3662A"/>
    <dbReference type="eggNOG" id="arCOG04314">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_178987_2_1_2"/>
    <dbReference type="OrthoDB" id="7620at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04457">
        <property type="entry name" value="S1_S28E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000145">
        <property type="entry name" value="30S ribosomal protein S28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00292">
        <property type="entry name" value="Ribosomal_eS28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000289">
        <property type="entry name" value="Ribosomal_eS28"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003080">
        <property type="entry name" value="PRK04007.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10769">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10769:SF3">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01200">
        <property type="entry name" value="Ribosomal_S28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_1000004124" description="Small ribosomal subunit protein eS28">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="75" mass="7989" checksum="68A7C8F168EAF5BF" modified="2005-11-08" version="1">MSAEEQEEGGATPAEVIEVVGETGMHGEAMQVKCRIQEGENQGRIIARNVLGPVRVGDVIQLRETAREADSIGGQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-05-29" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IR95</accession>
    <name>MPTA_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GTP cyclohydrolase MptA</fullName>
            <ecNumber evidence="1">3.5.4.39</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">GTP cyclohydrolase IV</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mptA</name>
        <name type="ordered locus">NP_2514A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Converts GTP to 7,8-dihydro-D-neopterin 2',3'-cyclic phosphate, the first intermediate in the biosynthesis of coenzyme methanopterin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP + H2O = 7,8-dihydroneopterin 2',3'-cyclic phosphate + formate + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25860"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58854"/>
            <dbReference type="EC" id="3.5.4.39"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="1">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; 5,6,7,8-tetrahydromethanopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP cyclohydrolase IV family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.39" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322974.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IR95"/>
    <dbReference type="SMR" id="Q3IR95"/>
    <dbReference type="STRING" id="348780.NP_2514A"/>
    <dbReference type="EnsemblBacteria" id="CAI49348">
        <property type="protein sequence ID" value="CAI49348"/>
        <property type="gene ID" value="NP_2514A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701470"/>
    <dbReference type="KEGG" id="nph:NP_2514A"/>
    <dbReference type="eggNOG" id="arCOG04301">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062816_1_0_2"/>
    <dbReference type="OrthoDB" id="53087at2157"/>
    <dbReference type="UniPathway" id="UPA00065"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003934">
        <property type="term" value="F:GTP cyclohydrolase I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044682">
        <property type="term" value="F:GTP cyclohydrolase IV activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001118">
        <property type="term" value="P:tetrahydromethanopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.270.10">
        <property type="entry name" value="Urate Oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01527_A">
        <property type="entry name" value="GTP_cyclohydrol_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003801">
        <property type="entry name" value="GTP_cyclohydrolase_FolE2/MptA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022840">
        <property type="entry name" value="GTP_cyclohydrolase_MptA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00294">
        <property type="entry name" value="GTP cyclohydrolase MptA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36445">
        <property type="entry name" value="GTP CYCLOHYDROLASE MPTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36445:SF1">
        <property type="entry name" value="GTP CYCLOHYDROLASE MPTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02649">
        <property type="entry name" value="GCHY-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000289542" description="GTP cyclohydrolase MptA">
        <location>
            <begin position="1"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="site" description="May be catalytically important" evidence="1">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01527"/>
        </source>
    </evidence>
    <sequence length="309" mass="34436" checksum="EC15795869273DDB" modified="2005-11-08" version="1">MSQQLPDVQASSPDVSVGLSRVGVTGVEKLVKLHRPNDRPIVLMAEFDVFVDLPSWRKGADMSRNMEVVDEMLEEAVSEPALSVEDVCGDVAERLIEKHDYTTQAEVRMEAEYMIREETPATDRPTQATADIIASATADEDGNTREEIGCHVVGMTVCPCSQGMSESRARDVLEGLDVDRDTIDAFLEEVPQPGHSQRGHATLTVETSGDPDVDLRTLIDIARDSMSARIYNLAKRPDEDHMTFESHSDAKFVEDCVRSMANGLLEEYPDLPDDTIVRMEQSNDESIHQHNAHAERVAEFGQLRREVRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IR97</accession>
    <name>Q3IR97_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KaiC domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_2510A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49346.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IR97"/>
    <dbReference type="STRING" id="348780.NP_2510A"/>
    <dbReference type="EnsemblBacteria" id="CAI49346">
        <property type="protein sequence ID" value="CAI49346"/>
        <property type="gene ID" value="NP_2510A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_2510A"/>
    <dbReference type="eggNOG" id="arCOG01173">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023669_1_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01124">
        <property type="entry name" value="KaiC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022420">
        <property type="entry name" value="Circ_KaiC_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014774">
        <property type="entry name" value="KaiC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010624">
        <property type="entry name" value="KaiC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03880">
        <property type="entry name" value="KaiC_arch_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637">
        <property type="entry name" value="UPF0273 PROTEIN TM_0370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637:SF1">
        <property type="entry name" value="UPF0273 PROTEIN TM_0370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06745">
        <property type="entry name" value="ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51146">
        <property type="entry name" value="KAIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KaiC" evidence="4">
        <location>
            <begin position="65"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49346.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="293" mass="32376" checksum="4E4A8ECEC02DF680" modified="2012-10-31" version="2">MSDDDWFEQDTEDSPDDGPSTDDFESAFDGEIGGDGPSTDDFESAFDAGSGGSEEFDEEFDSDIPRIDLGINGLDNMIQGGVPERSLMVVMGSAGTGKTTFGLQFLNEGIENGERGVYITLEETKDAVRRAATEKGWPFDEYMAENKLAVVDLDPIEMANSLTSIRGDLPRLIDAFGAERLVLDSVSLLEMMYDEQSARRTEVFDFTKALKSAGISTMVTSEVSDTDSYTSKYGIIEYLTDAVFVLQYVRSDFKETRLAVEIQKIRNANHSRETKPYEITSDGISVYQQASIF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ISR6</accession>
    <name>GATA_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamyl-tRNA(Gln) amidotransferase subunit A</fullName>
            <shortName evidence="1">Glu-ADT subunit A</shortName>
            <ecNumber evidence="1">6.3.5.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gatA</name>
        <name type="ordered locus">NP_1456A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the amidase family. GatA subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.7" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322454.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ISR6"/>
    <dbReference type="SMR" id="Q3ISR6"/>
    <dbReference type="STRING" id="348780.NP_1456A"/>
    <dbReference type="EnsemblBacteria" id="CAI48819">
        <property type="protein sequence ID" value="CAI48819"/>
        <property type="gene ID" value="NP_1456A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702359"/>
    <dbReference type="KEGG" id="nph:NP_1456A"/>
    <dbReference type="eggNOG" id="arCOG01717">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009600_0_3_2"/>
    <dbReference type="OrthoDB" id="7931at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030956">
        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00120">
        <property type="entry name" value="GatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004412">
        <property type="entry name" value="GatA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00132">
        <property type="entry name" value="gatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895:SF7">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT A, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000241184" description="Glutamyl-tRNA(Gln) amidotransferase subunit A">
        <location>
            <begin position="1"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="75"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="183"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="28"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="1">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="423" mass="43770" checksum="8B3872C20E1CF0CF" modified="2005-11-08" version="1">MSHNAFITEETIEPTDDGPLDGVTVAVKDNISTDGVRTTCGSEMLADYVPPYDATVVERLRDAGATIVGKTNMDEFGMGTTTETSAFGPTENPAAEGRVPGGSSGGSAAAVAAGDADLALGSDTGGSVRCPAAFCGVVGIKPTYGLVSRYGLVAYANSLEQIGPLAPTVEEAAELLDVIAGVDERDGTTREPPAGQPTYADAADGDVDGLTVGIPTELVDGADEGVRETFDDAVETLESAGATTQEVSLPSVETAVQAYYVIAMSEASSNLARFDGVRYGASGDEDGNWNESFAAAREAGFGEEVKRRILLGTYALSAGYHDKYYKQAQDARAWVKQDFDEAFESVDVLASPTMPVPPFERGESLEDPLKMYLADANTVPVNLANLPAISVPAGETADGPVGLQLVGPAFGEATLIRAGSALN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-12-06" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ITT7</accession>
    <name>SYA_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Alanine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Alanyl-tRNA synthetase</fullName>
            <shortName evidence="1">AlaRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">alaS</name>
        <name type="ordered locus">NP_0710A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Ala) + L-alanine + ATP = L-alanyl-tRNA(Ala) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:12540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:9923"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78497"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.7" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322082.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ITT7"/>
    <dbReference type="SMR" id="Q3ITT7"/>
    <dbReference type="STRING" id="348780.NP_0710A"/>
    <dbReference type="EnsemblBacteria" id="CAI48446">
        <property type="protein sequence ID" value="CAI48446"/>
        <property type="gene ID" value="NP_0710A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702499"/>
    <dbReference type="KEGG" id="nph:NP_0710A"/>
    <dbReference type="eggNOG" id="arCOG01255">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004485_4_0_2"/>
    <dbReference type="OrthoDB" id="7506at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006419">
        <property type="term" value="P:alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.310.40:FF:000001">
        <property type="entry name" value="Alanine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000056">
        <property type="entry name" value="Alanine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.980.10:FF:000004">
        <property type="entry name" value="Alanine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.54.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.550">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00036_A">
        <property type="entry name" value="Ala_tRNA_synth_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002318">
        <property type="entry name" value="Ala-tRNA-lgiase_IIc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018162">
        <property type="entry name" value="Ala-tRNA-ligase_IIc_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018164">
        <property type="entry name" value="Ala-tRNA-synth_IIc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022429">
        <property type="entry name" value="Ala-tRNA_lgiase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050058">
        <property type="entry name" value="Ala-tRNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03683">
        <property type="entry name" value="A-tRNA_syn_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00344">
        <property type="entry name" value="alaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777:SF9">
        <property type="entry name" value="ALANINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777">
        <property type="entry name" value="ALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01411">
        <property type="entry name" value="tRNA-synt_2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00980">
        <property type="entry name" value="TRNASYNTHALA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101353">
        <property type="entry name" value="Putative anticodon-binding domain of alanyl-tRNA synthetase (AlaRS)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000075269" description="Alanine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="893"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="894"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="623"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="627"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="726"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="730"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="932" mass="103353" checksum="73D472E450675543" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IU82</accession>
    <name>RL10E_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL16</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L10e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl10e</name>
        <name type="ordered locus">NP_0416A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL16 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321937.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IU82"/>
    <dbReference type="SMR" id="Q3IU82"/>
    <dbReference type="STRING" id="348780.NP_0416A"/>
    <dbReference type="EnsemblBacteria" id="CAI48299">
        <property type="protein sequence ID" value="CAI48299"/>
        <property type="gene ID" value="NP_0416A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703120"/>
    <dbReference type="KEGG" id="nph:NP_0416A"/>
    <dbReference type="eggNOG" id="arCOG04113">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084051_0_2_2"/>
    <dbReference type="OrthoDB" id="30538at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.10">
        <property type="entry name" value="Ribosomal protein L10e/L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00448">
        <property type="entry name" value="Ribosomal_uL16_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047873">
        <property type="entry name" value="Ribosomal_uL16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022981">
        <property type="entry name" value="Ribosomal_uL16_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018255">
        <property type="entry name" value="Ribosomal_uL16_CS_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001197">
        <property type="entry name" value="Ribosomal_uL16_euk_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036920">
        <property type="entry name" value="Ribosomal_uL16_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003239">
        <property type="entry name" value="PRK04199.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003241">
        <property type="entry name" value="PRK04199.1-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11726">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00252">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005590">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54686">
        <property type="entry name" value="Ribosomal protein L16p/L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01257">
        <property type="entry name" value="RIBOSOMAL_L10E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_1000026193" description="Large ribosomal subunit protein uL16">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="177" mass="19995" checksum="A7A725C2E1E01DC1" modified="2005-11-08" version="1">MSDKPASMYRDIDKPAYTRREYITGIPGSKVAQYKMGNFEADPEDYEVQISLIVDEEVQIRHGSLEASRLSANRRMLKELGEDGDYKMILRKFPHQVIRENKQATGAGADRVSDGMRQAFGKIVGTAARINKGERVFTIWCHPEDADAAKDAFRRAYNKISPPCTIKVERGEELLIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IUE6</accession>
    <name>Q3IUE6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">6-phosphogluconate dehydrogenase, NAD(+)-dependent, decarboxylating</fullName>
            <shortName evidence="4">6PGDH</shortName>
            <ecNumber evidence="4">1.1.1.343</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">gndA</name>
        <name evidence="6" type="ordered locus">NP_0286A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NAD to NADH.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>6-phospho-D-gluconate + NAD(+) = D-ribulose 5-phosphate + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:33023"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58759"/>
            <dbReference type="EC" id="1.1.1.343"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Carbohydrate degradation; pentose phosphate pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the 6-phosphogluconate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.343" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321873.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IUE6"/>
    <dbReference type="STRING" id="348780.NP_0286A"/>
    <dbReference type="EnsemblBacteria" id="CAI48234">
        <property type="protein sequence ID" value="CAI48234"/>
        <property type="gene ID" value="NP_0286A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702767"/>
    <dbReference type="KEGG" id="nph:NP_0286A"/>
    <dbReference type="eggNOG" id="arCOG00248">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024540_0_0_2"/>
    <dbReference type="OrthoDB" id="23890at2157"/>
    <dbReference type="UniPathway" id="UPA00115"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004616">
        <property type="term" value="F:phosphogluconate dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019521">
        <property type="term" value="P:D-gluconate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009051">
        <property type="term" value="P:pentose-phosphate shunt, oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02047">
        <property type="entry name" value="6PGDH_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004849">
        <property type="entry name" value="6DGDH_YqeC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032883">
        <property type="entry name" value="6PGDH_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006114">
        <property type="entry name" value="6PGDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006183">
        <property type="entry name" value="Pgluconate_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00872">
        <property type="entry name" value="gnd_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007161">
        <property type="entry name" value="PRK09599.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11811">
        <property type="entry name" value="6-PHOSPHOGLUCONATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00393">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00076">
        <property type="entry name" value="6PGDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01350">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0311">Gluconate utilization</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphogluconate dehydrogenase C-terminal" evidence="5">
        <location>
            <begin position="165"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="7"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="67"/>
            <end position="69"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="118"/>
            <end position="120"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="172"/>
            <end position="173"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI48234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="299" mass="32287" checksum="7A9CFC4CD948952F" modified="2005-11-08" version="1">MQIGVIGLGRMGQIVVERTLDGGHDVVAFDLDDAAVSQAADAGATPADSIEDLVSRLPDEKRIWLMVPAGDAVDATLDDLDPHLDGDDVVVDGGNSHFERSVERAAATAAAYLDCGTSGGPAGAELGFSLMVGGPEWAYEEFTPVFDAVATGPDGHDRMGPSGSGHYVKMVHNGVEYALMQAYGEGFELLYEGRYDLDMEAVARTWNNGAVIRSWLLELCEEAFREEGNDLGDVADHIAGGSTGTWTVEEALEQEVSVPLIYEALGERFDSRTDEDGKFSRRLANRLRYGFGRHEVKRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETB3</accession>
    <name>A0A1U7ETB3_NATPD</name>
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        <submittedName>
            <fullName evidence="2">ABC-type transport system periplasmic substrate-binding protein (Probable substrate iron/cobalamin)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0064A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321762.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETB3"/>
    <dbReference type="STRING" id="348780.NP_0064A"/>
    <dbReference type="EnsemblBacteria" id="CAI48123">
        <property type="protein sequence ID" value="CAI48123"/>
        <property type="gene ID" value="NP_0064A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700750"/>
    <dbReference type="KEGG" id="nph:NP_0064A"/>
    <dbReference type="eggNOG" id="arCOG04233">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038034_9_1_2"/>
    <dbReference type="OrthoDB" id="9784at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1980">
        <property type="entry name" value="Nitrogenase molybdenum iron protein domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051030">
        <property type="entry name" value="Vitamin_B12-ABC_binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42860">
        <property type="entry name" value="VITAMIN B12-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42860:SF1">
        <property type="entry name" value="VITAMIN B12-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53807">
        <property type="entry name" value="Helical backbone' metal receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="1">
        <location>
            <begin position="4"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="304" mass="32586" checksum="74B4165E03EC03D1" modified="2017-05-10" version="1">MPPTVVSLLPSATEIVAALGVDPAATSHECDYPPSVADTPTVVESRIDADADSETIDEQVHEAETDGGVYAIDRETLAAVDPDIVISQGICEVCAVDTVQVEAAIADLGLDCRLVTTDPHSVDDILGDIERIGAALGKDRRANRLAAALESRMDRVADRTAGRSRRPTVAVLDWLSPPMVAGHWVPELVERAGGEYGLADPGDASMPREWATIREYDPDVLVAAPCGFDLEQTADNRTDLTERPGWNDLRAVRNGRVYAMDGHHLTNRPGIRVLETLETLAALCAPTERDSPAPWMARPLAADG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETC4</accession>
    <name>A0A1U7ETC4_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">NP_0090A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48136.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939374.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETC4"/>
    <dbReference type="STRING" id="348780.NP_0090A"/>
    <dbReference type="EnsemblBacteria" id="CAI48136">
        <property type="protein sequence ID" value="CAI48136"/>
        <property type="gene ID" value="NP_0090A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700759"/>
    <dbReference type="KEGG" id="nph:NP_0090A"/>
    <dbReference type="eggNOG" id="arCOG02329">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02364">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02369">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06796">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010703_0_0_2"/>
    <dbReference type="OrthoDB" id="106630at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000156">
        <property type="term" value="F:phosphorelay response regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007234">
        <property type="term" value="P:osmosensory signaling via phosphorelay pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00075">
        <property type="entry name" value="HATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050351">
        <property type="entry name" value="BphY/WalK/GraS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013656">
        <property type="entry name" value="PAS_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42878:SF13">
        <property type="entry name" value="HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42878">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13185">
        <property type="entry name" value="GAF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08448">
        <property type="entry name" value="PAS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13188">
        <property type="entry name" value="PAS_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="8">
        <location>
            <begin position="286"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="8">
        <location>
            <begin position="537"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="9">
        <location>
            <begin position="611"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="792"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="646"/>
            <end position="673"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI48136.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="1013" mass="111244" checksum="48CC66EF20EE3801" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETC7</accession>
    <name>A0A1U7ETC7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0128A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321794.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETC7"/>
    <dbReference type="EnsemblBacteria" id="CAI48155">
        <property type="protein sequence ID" value="CAI48155"/>
        <property type="gene ID" value="NP_0128A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700764"/>
    <dbReference type="KEGG" id="nph:NP_0128A"/>
    <dbReference type="eggNOG" id="arCOG04717">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1109537_0_0_2"/>
    <dbReference type="OrthoDB" id="201232at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043940">
        <property type="entry name" value="DUF5781"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19093">
        <property type="entry name" value="DUF5781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="251" mass="27780" checksum="2F2FE17C94FEECE7" modified="2017-05-10" version="1">MDIRVRGDGPAAPFLSARDMLDTEYGLERPVVVDIRDDPDERTMVGHYDDRHELVVSRRAATSAMARELALHEFAHMARYEQAHPSHLQSTEEALFLALAGRSVEQRKLSHCYQIANHMKDIYADDITLDIGPTDKLVSFLESSLARALADSPPSPPEDGRRLRPGADPEITVVNAAFALALLERHDAIDADHRLYDLASAAAADAPSVSLSAFKRRFRSLEDDPDESAYRKALVDATREYVLSDTGQAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETG3</accession>
    <name>A0A1U7ETG3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Receiver/sensor box histidine kinase</fullName>
            <ecNumber evidence="6">2.7.13.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">NP_0188A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48185.3"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939382.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETG3"/>
    <dbReference type="STRING" id="348780.NP_0188A"/>
    <dbReference type="EnsemblBacteria" id="CAI48185">
        <property type="protein sequence ID" value="CAI48185"/>
        <property type="gene ID" value="NP_0188A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700782"/>
    <dbReference type="KEGG" id="nph:NP_0188A"/>
    <dbReference type="eggNOG" id="arCOG02367">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02388">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_58_2"/>
    <dbReference type="OrthoDB" id="230688at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004673">
        <property type="term" value="F:protein histidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00075">
        <property type="entry name" value="HATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00156">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052155">
        <property type="entry name" value="Biofilm_reg_signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44757:SF2">
        <property type="entry name" value="BIOFILM ARCHITECTURE MAINTENANCE PROTEIN MBAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44757">
        <property type="entry name" value="DIGUANYLATE CYCLASE DGCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="9"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="4">
        <location>
            <begin position="137"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="4">
        <location>
            <begin position="254"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="5">
        <location>
            <begin position="325"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="2">
        <location>
            <begin position="383"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI48185.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="601" mass="66347" checksum="545CAD606F6B8D15" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETH2</accession>
    <name>A0A1U7ETH2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Phytoene dehydrogenase (Phytoene desaturase)</fullName>
            <ecNumber evidence="5">1.14.99.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">crtI2</name>
        <name evidence="5" type="ordered locus">NP_0204A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carotenoid biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="1.14.99.-" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321832.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETH2"/>
    <dbReference type="STRING" id="348780.NP_0204A"/>
    <dbReference type="EnsemblBacteria" id="CAI48193">
        <property type="protein sequence ID" value="CAI48193"/>
        <property type="gene ID" value="NP_0204A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702634"/>
    <dbReference type="KEGG" id="nph:NP_0204A"/>
    <dbReference type="eggNOG" id="arCOG01521">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019722_2_1_2"/>
    <dbReference type="OrthoDB" id="40741at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016117">
        <property type="term" value="P:carotenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014105">
        <property type="entry name" value="Carotenoid/retinoid_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02734">
        <property type="entry name" value="crtI_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43734">
        <property type="entry name" value="PHYTOENE DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43734:SF1">
        <property type="entry name" value="PHYTOENE DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0125">Carotenoid biosynthesis</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="4">
        <location>
            <begin position="27"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI48193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="510" mass="55276" checksum="58F40CFFE8A65772" modified="2017-05-10" version="1">MPATDTTQHARCPLDGQSATVVGGGFGGLAAACHLAADGAEVRLLEQHDRLGGVAGRIETDGFRIDTGPSWYLMPGAFERFFGHFDREPSAFYDLERLDPHYRVFWKDGDRVDVPDDPEAVAELFERYEPGAGNAFRAYLDSAEHAYEVGVDEFVYEDRSRVRDCLDPRLLRAVRGLTLLSSMDEYVADYFENPKLRQLVQYTLVFLGGAPHNTPAIYTLMSHVDFNLGVYHPTGGIASVVDGVAELARDLGVDIETGTPVSGLDPADNAITVSTDAGTHRADTVVANAAPAHVDRDLLPDGVAEYGDDYWSDRTYAPSAFMLYLGVEGDVEPLYHHTLVLPTDWDDHFRQIFETPAWPDDPAYYINVPSSTDPTMAPDGHEAVVVLVPIAPGLDDGPAVRETFREQVLNDIATHTGVDLRDRIVLERTACVSEFGQRFGAPQGTALGLAHTLSQTGPLRPSIRSSVSDSLFYAGSYTSPGIGVPMCLTSGEHAAAAVRADATGGLFSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETH6</accession>
    <name>A0A1U7ETH6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0234A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321847.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETH6"/>
    <dbReference type="STRING" id="348780.NP_0234A"/>
    <dbReference type="EnsemblBacteria" id="CAI48208">
        <property type="protein sequence ID" value="CAI48208"/>
        <property type="gene ID" value="NP_0234A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700796"/>
    <dbReference type="KEGG" id="nph:NP_0234A"/>
    <dbReference type="eggNOG" id="arCOG01572">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1248314_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008910">
        <property type="entry name" value="MSC_TM_helix"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05552">
        <property type="entry name" value="MS_channel_1st_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="191"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="221" mass="21846" checksum="76AE233A54AF1BC3" modified="2017-05-10" version="1">MLGQLLDIAEQRFAEAIVTIIDLLPRLILGILILAVAFGVAGTIAGTVRSGLSQTGLFDAVSQTPIAGNASANNIAAGTGILISGYVKLIGAAIAVAALEIGGISGLAVSVGWYVTFALGAAAVVAIGSVLATVVGGRAAAWEPVRGTAAAPILGGLTQAFLYLVAGVIALDLLDLRTGIVFAVVEPLARGLGLALAVGLGAAIVIAYSDDIAAYLDNIGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETI5</accession>
    <name>A0A1U7ETI5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Endonuclease NucS</fullName>
            <ecNumber evidence="6">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 10" type="primary">nucS</name>
        <name evidence="10" type="ordered locus">NP_0238A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Cleaves both 3' and 5' ssDNA extremities of branched DNA structures.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NucS endonuclease family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321849.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETI5"/>
    <dbReference type="STRING" id="348780.NP_0238A"/>
    <dbReference type="EnsemblBacteria" id="CAI48210">
        <property type="protein sequence ID" value="CAI48210"/>
        <property type="gene ID" value="NP_0238A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700798"/>
    <dbReference type="KEGG" id="nph:NP_0238A"/>
    <dbReference type="eggNOG" id="arCOG01304">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069350_1_0_2"/>
    <dbReference type="OrthoDB" id="15177at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22341">
        <property type="entry name" value="NucS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.180.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00722">
        <property type="entry name" value="NucS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002793">
        <property type="entry name" value="Endonuclease_NucS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048301">
        <property type="entry name" value="NucS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048302">
        <property type="entry name" value="NucS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049173">
        <property type="entry name" value="NucS_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003270">
        <property type="entry name" value="PRK04247.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38814">
        <property type="entry name" value="ENDONUCLEASE NUCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38814:SF1">
        <property type="entry name" value="ENDONUCLEASE NUCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01939">
        <property type="entry name" value="NucS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21003">
        <property type="entry name" value="NucS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 6 10" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4 6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease NucS N-terminal PH-like" evidence="9">
        <location>
            <begin position="33"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Endonuclease NucS C-terminal" evidence="8">
        <location>
            <begin position="137"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI48210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="246" mass="26651" checksum="EDD7BF7DA95CF647" modified="2017-05-10" version="1">MTDDPSEDATRTLAEPAVGPASSLVADGLASDALVTLFGRCTVDYEGRAASELGPGDRHVMLKPDGAALVHTDEGQKPVNWQPPGCTHECRVDDGALVIESRRETPDERLEIRFSDVAHVAVFRPTDEETLELEGTEADLKDRILSEPSLVESGFRPLATERETPAGAVDVYGEDSEGRTTVLELKRRRVGPDAVGQLDRYVAALERDLHADAEVRGILVAPSVTERARSLLAEQGLEFVSLTPRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETK1</accession>
    <name>A0A1U7ETK1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0278A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321869.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETK1"/>
    <dbReference type="STRING" id="348780.NP_0278A"/>
    <dbReference type="EnsemblBacteria" id="CAI48230">
        <property type="protein sequence ID" value="CAI48230"/>
        <property type="gene ID" value="NP_0278A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700810"/>
    <dbReference type="KEGG" id="nph:NP_0278A"/>
    <dbReference type="eggNOG" id="arCOG00381">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1217616_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="227" mass="24798" checksum="73985E6EFBBE06DD" modified="2017-05-10" version="1">MRRRALLTSCVTVAAVSISGCFDDGESPSGGTDPADNNTEHNGRNESTETDEENQSGDYEDGGESATSDSGPTCDVPTHEFIDGEDPPVMDFVEWEPVSEIGETGAQMTRTITREPNIRVLNEGGVVISDGEPYELRINSDSIALKAPDGDRRTYGVGDKLYYRHATWRVDDVSDGSVSIEYVDSPTDLQVDWTEGDFVNVDGGTFVVGVEPHERLFLCRYSKEEGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETL0</accession>
    <name>A0A1U7ETL0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CCA-adding enzyme</fullName>
            <ecNumber evidence="10">2.7.7.72</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">CCA tRNA nucleotidyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">tRNA CCA-pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">tRNA adenylyl-/cytidylyl- transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">tRNA nucleotidyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">tRNA-NT</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10 14" type="primary">cca</name>
        <name evidence="14" type="ordered locus">NP_0320A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate. tRNA 3'-terminal CCA addition is required both for tRNA processing and repair. Also involved in tRNA surveillance by mediating tandem CCA addition to generate a CCACCA at the 3' terminus of unstable tRNAs. While stable tRNAs receive only 3'-terminal CCA, unstable tRNAs are marked with CCACCA and rapidly degraded.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14433"/>
            <dbReference type="Rhea" id="RHEA-COMP:10465"/>
            <dbReference type="Rhea" id="RHEA-COMP:10468"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:74896"/>
            <dbReference type="ChEBI" id="CHEBI:83071"/>
            <dbReference type="EC" id="2.7.7.72"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a tRNA with a 3' CCA end + 2 CTP + ATP = a tRNA with a 3' CCACCA end + 3 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:76235"/>
            <dbReference type="Rhea" id="RHEA-COMP:10468"/>
            <dbReference type="Rhea" id="RHEA-COMP:18655"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:83071"/>
            <dbReference type="ChEBI" id="CHEBI:195187"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="10">A single active site specifically recognizes both ATP and CTP and is responsible for their addition.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Archaeal CCA-adding enzyme subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.72" evidence="10"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="RefSeq" id="WP_011321889.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A1U7ETL0"/>
    <dbReference type="STRING" id="348780.NP_0320A"/>
    <dbReference type="EnsemblBacteria" id="CAI48251">
        <property type="protein sequence ID" value="CAI48251"/>
        <property type="gene ID" value="NP_0320A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702586"/>
    <dbReference type="KEGG" id="nph:NP_0320A"/>
    <dbReference type="eggNOG" id="arCOG04249">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044679_0_0_2"/>
    <dbReference type="OrthoDB" id="7378at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:CCA tRNA nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160016">
        <property type="term" value="F:CCACCA tRNA nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
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    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042245">
        <property type="term" value="P:RNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001680">
        <property type="term" value="P:tRNA 3'-terminal CCA addition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="entry name" value="NT_2-5OAS_ClassI-CCAase"/>
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        <property type="entry name" value="Archaeal tRNA CCA-adding enzyme catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.30">
        <property type="entry name" value="CCA tRNA nucleotidyltransferase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.590">
        <property type="entry name" value="Poly(A) polymerase predicted RNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CCA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048833">
        <property type="entry name" value="CAA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008229">
        <property type="entry name" value="CCA-adding_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042090">
        <property type="entry name" value="CCA_tRNA_nucleotrans_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006116">
        <property type="entry name" value="NT_2-5OAS_ClassI-CCAase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011068">
        <property type="entry name" value="NuclTrfase_I-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015329">
        <property type="entry name" value="tRNA_NucTransf2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03671">
        <property type="entry name" value="cca_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39643">
        <property type="entry name" value="CCA-ADDING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39643:SF1">
        <property type="entry name" value="CCA-ADDING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21133">
        <property type="entry name" value="CAA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09249">
        <property type="entry name" value="tRNA_NucTransf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005335">
        <property type="entry name" value="CCA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55003">
        <property type="entry name" value="PAP/Archaeal CCA-adding enzyme, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 10 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0692">RNA repair</keyword>
    <keyword evidence="9 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 10 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 10" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Polymerase nucleotidyl transferase" evidence="11">
        <location>
            <begin position="45"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA nucleotidyltransferase substrate binding" evidence="12">
        <location>
            <begin position="152"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="CCA-adding enzyme C-terminal" evidence="13">
        <location>
            <begin position="281"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
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        </ligand>
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        <location>
            <position position="116"/>
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        <ligand>
            <name>Mg(2+)</name>
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        </ligand>
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        </location>
        <ligand>
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        </ligand>
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            <position position="139"/>
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        <ligand>
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        </ligand>
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        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>ATP</name>
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            <position position="158"/>
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        <ligand>
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        <location>
            <position position="167"/>
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        <ligand>
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            <position position="167"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01264"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF09249"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF21133"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI48251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="448" mass="48695" checksum="9AEDF392746DBA65" modified="2017-05-10" version="1">MSLESVLSSVRDRVVPEASEQEALQAAVAKLTARAEAAITDLPVTADTRLVGSTARGTWLAGDRDIDLFVRFPPDLDRERLETYGLEVGHAVLPDGHEEFAEHPYVKGSYRGFDVDCVPCYAVADATDIRSAVDRTPFHTEYLDGRIERLADDVRLAKAFLTGIGVYGSDLRTRGFSGFLTELLVVEYGGFEALVEAAADWQPPVRLDPKSHGQRSFDDPLVVVDPTDPERNVAAVCSAANVARLIHYARDLQTDPRASLFEPADPEPLSPAAVRKRVSERGTTPVAVRFDAPDIVDDQLYPQLDKSLNGIEGALDRAGFDPLRSARFADESAVLFVECAVAERPAIEHHAGPPVGVRDHAEGFYDAYADSDAVSGPFIDEDGRYAVERARDARTPAALLREQLFDVSLGTHVADALADDYEILSGEEVASLAPSFGTELAAYFDPKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETL8</accession>
    <name>A0A1U7ETL8_NATPD</name>
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        <submittedName>
            <fullName evidence="1">Probable SepF protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">sepF</name>
        <name evidence="1" type="ordered locus">NP_0342A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
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            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321900.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETL8"/>
    <dbReference type="STRING" id="348780.NP_0342A"/>
    <dbReference type="EnsemblBacteria" id="CAI48262">
        <property type="protein sequence ID" value="CAI48262"/>
        <property type="gene ID" value="NP_0342A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700824"/>
    <dbReference type="KEGG" id="nph:NP_0342A"/>
    <dbReference type="eggNOG" id="arCOG02263">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131897_0_0_2"/>
    <dbReference type="OrthoDB" id="56189at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.150">
        <property type="entry name" value="SepF-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007561">
        <property type="entry name" value="Cell_div_SepF/SepF-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038594">
        <property type="entry name" value="SepF-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012426">
        <property type="entry name" value="SepF_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04472">
        <property type="entry name" value="SepF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019313">
        <property type="entry name" value="UCP019313"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="118" mass="12788" checksum="F3D56E4DAE092F4F" modified="2017-05-10" version="1">MGLMSKLLGGNSHSAEDYVELDVDDFDASEAGAASVQVRFTDISEKNDVIDIKDAVYDGDIVIADIIRHTTSDRTMEHILDELKQVANEVGGDIVQKEDDQLIITPAGVGISRSKIGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETN8</accession>
    <name>A0A1U7ETN8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0396A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321927.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETN8"/>
    <dbReference type="STRING" id="348780.NP_0396A"/>
    <dbReference type="EnsemblBacteria" id="CAI48289">
        <property type="protein sequence ID" value="CAI48289"/>
        <property type="gene ID" value="NP_0396A"/>
    </dbReference>
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            <begin position="112"/>
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    </feature>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI48289.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
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    </evidence>
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</entry>
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    <accession>A0A1U7ETW0</accession>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
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            <dbReference type="PubMed" id="16169924"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <dbReference type="EnsemblBacteria" id="CAI48365">
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    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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            <dbReference type="EMBL" id="CAI48365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="129" mass="14162" checksum="FA4A39628A95E312" modified="2017-05-10" version="1">MNVRDAVEADAERLAALTDAPTDVMRNLIHDRTVRVADDDETVEGFVSFDVQAGTVHVTQLEGDCELCERLLEEPARFAACEEMDVELLVPETDTDAADAAVEAGFEECGRGPRFDGVGTTRYEWQPDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETY7</accession>
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        <submittedName>
            <fullName evidence="9">Receiver/sensor box histidine kinase</fullName>
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        <name evidence="9" type="ordered locus">NP_0626A</name>
    </gene>
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        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <lineage>
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            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
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    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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            <authorList>
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                <person name="Pfeiffer F."/>
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            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
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        <property type="gene ID" value="NP_0626A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700909"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="HisK_dim/P_dom"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
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        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08448">
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        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
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        <property type="entry name" value="GAF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
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    </dbReference>
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        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
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        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Response regulatory" evidence="6">
        <location>
            <begin position="11"/>
            <end position="128"/>
        </location>
    </feature>
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        <location>
            <begin position="144"/>
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        </location>
    </feature>
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        <location>
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    </feature>
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        </location>
    </feature>
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    </feature>
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    </feature>
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        <location>
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            <end position="687"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="5">
        <location>
            <begin position="851"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="4">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI48404.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="1047" mass="115723" checksum="4CC3C1DA28E5DE0E" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETY8</accession>
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        <recommendedName>
            <fullName evidence="2">DUF8081 domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="3" type="ordered locus">NP_0636A</name>
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        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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            <authorList>
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
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            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
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    </dbReference>
    <dbReference type="GeneID" id="3700914"/>
    <dbReference type="KEGG" id="nph:NP_0636A"/>
    <dbReference type="eggNOG" id="arCOG07588">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_178209_0_0_2"/>
    <dbReference type="OrthoDB" id="291179at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058394">
        <property type="entry name" value="DUF8081"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26297">
        <property type="entry name" value="DUF8081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8081" evidence="2">
        <location>
            <begin position="5"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48409.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="98" mass="10739" checksum="A3A737C8C0A3725E" modified="2017-05-10" version="1">MEDGYVVVIKPSARRVSRAVGEWVAESGRSREFDSKALARRWAKAAAPQGKTLWVQDAHPLDPSPADGYLLARRSRLRGNEPERPGEQAPLSTAAEDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU32</accession>
    <name>A0A1U7EU32_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ArNOG08307 family NADH-binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">nolA2</name>
        <name evidence="2" type="ordered locus">NP_0704A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322079.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU32"/>
    <dbReference type="STRING" id="348780.NP_0704A"/>
    <dbReference type="EnsemblBacteria" id="CAI48443">
        <property type="protein sequence ID" value="CAI48443"/>
        <property type="gene ID" value="NP_0704A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702980"/>
    <dbReference type="KEGG" id="nph:NP_0704A"/>
    <dbReference type="eggNOG" id="arCOG03015">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_6_5_2"/>
    <dbReference type="OrthoDB" id="358920at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051207">
        <property type="entry name" value="ComplexI_NDUFA9_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126:SF11">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 39 KDA SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="1">
        <location>
            <begin position="7"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="296" mass="31841" checksum="BD6659587770AEC2" modified="2017-05-10" version="1">MRVLVAGATGFVGSRLVASLVDHGHDVVALTRDAERYDKPEGVDVVEGDLLDPPLELPSADAAYYLVHSMGAGGDFAARDRRAATSFAEAAEQAGIGRVIYLGGLGDEGDDLSEHLRSRREVEQVLGERAFELTTLRAAVIVGSGSASFELIRQLADRLPVMVTPRWVRTDCQPIYIDDVVAYLVGVLEAPATAGETFEIGGPKVLTYEDMLRTVAAQQGHRLRIVPVPVLSPWLSAHWLRLFTDVPAGVARPLVAGLRNAVTVSERRIDEHVEVELTPFDEAVQRALADGESGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU38</accession>
    <name>A0A1U7EU38_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type transport system permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">abc27p</name>
        <name evidence="7" type="ordered locus">NP_0746A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322100.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU38"/>
    <dbReference type="STRING" id="348780.NP_0746A"/>
    <dbReference type="EnsemblBacteria" id="CAI48464">
        <property type="protein sequence ID" value="CAI48464"/>
        <property type="gene ID" value="NP_0746A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702448"/>
    <dbReference type="KEGG" id="nph:NP_0746A"/>
    <dbReference type="eggNOG" id="arCOG01467">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039483_3_0_2"/>
    <dbReference type="OrthoDB" id="147058at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052522">
        <property type="entry name" value="ABC-2_transport_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047817">
        <property type="entry name" value="ABC2_TM_bact-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011648">
        <property type="entry name" value="PRK15066.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43332:SF2">
        <property type="entry name" value="INNER MEMBRANE TRANSPORT PERMEASE YADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43332">
        <property type="entry name" value="INNER MEMBRANE TRANSPORT PERMEASE YADH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006648">
        <property type="entry name" value="DrrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00164">
        <property type="entry name" value="ABC2TRNSPORT"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="121"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="155"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-2" evidence="6">
        <location>
            <begin position="37"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="271" mass="29449" checksum="E33D08B771B5C444" modified="2017-05-10" version="1">MSGDAATDADAGRAGPGGVFGVGFRSLLRREILRFVRRPRNTFAPPAITNVLYFAVFGVILGQRIDDIAGFDYIVFLLPGLVVLGAISNAFENASFSIFHGRWNEYIHETLTAPLSYSSMVLAYVFAAALRGIIVGLIIVAVGLVFTTVPVNEPLYLAAFMVVVPLLFASLGVIGGLVAEDFDDLTVMNQFILRPLVFFGAVFYSLEILPPLYRTLSLLNPMVYMVNGVRYGFLGYQEVDPLASLAVLSGLTAAVIVLDIYLFKRGYGLID</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU87</accession>
    <name>A0A1U7EU87_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0866A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322160.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU87"/>
    <dbReference type="EnsemblBacteria" id="CAI48524">
        <property type="protein sequence ID" value="CAI48524"/>
        <property type="gene ID" value="NP_0866A"/>
    </dbReference>
    <dbReference type="GeneID" id="41345076"/>
    <dbReference type="KEGG" id="nph:NP_0866A"/>
    <dbReference type="eggNOG" id="arCOG11364">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2857191_0_0_2"/>
    <dbReference type="OrthoDB" id="226125at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="64" mass="7132" checksum="2CB2990EAC46B524" modified="2017-05-10" version="1">MSTNPSMSEPRPTTEITARGRSDPWAAADPQPSLPATDIAPRQFEPTARSRLRNLMDKENAYCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU97</accession>
    <name>A0A1U7EU97_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Major facilitator superfamily transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">tp20</name>
        <name evidence="3" type="ordered locus">NP_0888A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322171.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU97"/>
    <dbReference type="STRING" id="348780.NP_0888A"/>
    <dbReference type="EnsemblBacteria" id="CAI48535">
        <property type="protein sequence ID" value="CAI48535"/>
        <property type="gene ID" value="NP_0888A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703296"/>
    <dbReference type="KEGG" id="nph:NP_0888A"/>
    <dbReference type="eggNOG" id="arCOG00130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056682_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015108">
        <property type="term" value="F:chloride transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23521">
        <property type="entry name" value="TRANSPORTER MFS SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23521:SF2">
        <property type="entry name" value="TRANSPORTER MFS SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="243"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="297"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="331"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="359"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="2">
        <location>
            <begin position="206"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="396" mass="40127" checksum="5AB23AC30A581AF9" modified="2017-05-10" version="1">MSRSERVRLAAVVFAVLFTQLLVYPGVDRLVDVLGGEPTVDAGTAFLGVQLLAFVLFSAVWGALSDRAGRRTPFIVLGAAGGAAAYLVLVGIVVAGIGDFRTALVVRFVEGAFTIGAFSLSITMLMDLDGGHGRNMGAAGIAIGAGAALGAPVGGQLYELGTLAPIVASAVLLVGVAAVVATIPDRAPDGGRSLRDAVDCVRRRPTLFVPFAFGFADRFAAGFFGLIGTFYFRDAFDLSPSEIGLLLACFFAPFALLQYPLGTLSDRIGRVVPIVVGSAGFGLAILGVGLAPTAALAAVGMVAVGVLGALVAPATMALVTDIAAPDERGAAMGGFNIFGSLGFLGGIIVGGALATRFDYFAAFLVAGGLEIVIAVLALPVLVRTVSDPTATFGSPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUI9</accession>
    <name>A0A1U7EUI9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HTH domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1102A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322278.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUI9"/>
    <dbReference type="STRING" id="348780.NP_1102A"/>
    <dbReference type="EnsemblBacteria" id="CAI48642">
        <property type="protein sequence ID" value="CAI48642"/>
        <property type="gene ID" value="NP_1102A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701052"/>
    <dbReference type="KEGG" id="nph:NP_1102A"/>
    <dbReference type="eggNOG" id="arCOG02756">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2313867_0_0_2"/>
    <dbReference type="OrthoDB" id="325082at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25212">
        <property type="entry name" value="HVO_A0114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="99" mass="11299" checksum="5599595AE7BFB4DF" modified="2017-05-10" version="1">MSDDIVVRLAANGDEETLQFDGESVDRLCRPTSIELLRTVADEEPASIRETARLVDRDVRQVHDNLWKLGELGLVEFDRSGRGHRPVVDWDRIDVEIDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUK2</accession>
    <name>A0A1U7EUK2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cobalt-factor-III C17-methyltransferase</fullName>
            <ecNumber evidence="8">2.1.1.272</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cbiH1</name>
        <name evidence="8" type="synonym">cobJ1</name>
        <name evidence="8" type="ordered locus">NP_1116A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.272" evidence="8"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUK2"/>
    <dbReference type="STRING" id="348780.NP_1116A"/>
    <dbReference type="EnsemblBacteria" id="CAI48649">
        <property type="protein sequence ID" value="CAI48649"/>
        <property type="gene ID" value="NP_1116A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_1116A"/>
    <dbReference type="eggNOG" id="arCOG00647">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047948_2_0_2"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11646">
        <property type="entry name" value="Precorrin_3B_C17_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006363">
        <property type="entry name" value="Cbl_synth_CobJ/CibH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051810">
        <property type="entry name" value="Precorrin_MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47036">
        <property type="entry name" value="COBALT-FACTOR III C(17)-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47036:SF1">
        <property type="entry name" value="COBALT-FACTOR III C(17)-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="3 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="7">
        <location>
            <begin position="11"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI48649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="283" mass="30732" checksum="486E2BFCE56C9042" modified="2017-05-10" version="1">MTASDGESMGTLYIVGIGPGLPHAMTQRARDVIQTADCVIASNLYQEFLRRDGTLPPEAETDGGTATRPNGTEQEIVRSSMGRQVELAREAFERVRNGEDVAHVSGGDPNVYGKSDLVFLMADEEDADDIPIEVVPGVTAALGGAANLGAPLSNDFCTVSLSDKWRGWDEIEEKLRAAAIAGFVIVLYNCWRDYERAIEVIREERADDVPVAIFNDAGRGEAGRNVDDETHTITTLGAATDHDEKVGGMGTSILVGTHESHEWENDYETYLVTPRGGREVEDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUK4</accession>
    <name>A0A1U7EUK4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATP-dependent cobaltochelatase subunit CobN</fullName>
            <ecNumber evidence="2">6.6.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cobN</name>
        <name evidence="2" type="ordered locus">NP_1092A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.6.1.2" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322273.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="STRING" id="348780.NP_1092A"/>
    <dbReference type="EnsemblBacteria" id="CAI48637">
        <property type="protein sequence ID" value="CAI48637"/>
        <property type="gene ID" value="NP_1092A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702619"/>
    <dbReference type="KEGG" id="nph:NP_1092A"/>
    <dbReference type="eggNOG" id="arCOG03022">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002017_1_2_2"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="P:cobalamin biosynthetic process"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cobalto_CobN"/>
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        <property type="entry name" value="CobN/Mg_chltase"/>
    </dbReference>
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        <property type="entry name" value="cobalto_cobN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44119:SF7">
        <property type="entry name" value="MAGNESIUM CHELATASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44119">
        <property type="entry name" value="MAGNESIUM-CHELATASE SUBUNIT CHLH, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02514">
        <property type="entry name" value="CobN-Mg_chel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobN/magnesium chelatase" evidence="1">
        <location>
            <begin position="109"/>
            <end position="1266"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="1299" mass="141298" checksum="8B1EA8BF731501F1" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUM3</accession>
    <name>A0A1U7EUM3_NATPD</name>
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        <recommendedName>
            <fullName evidence="3">DUF7312 domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="4" type="ordered locus">NP_1190A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
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            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322322.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUM3"/>
    <dbReference type="STRING" id="348780.NP_1190A"/>
    <dbReference type="EnsemblBacteria" id="CAI48686">
        <property type="protein sequence ID" value="CAI48686"/>
        <property type="gene ID" value="NP_1190A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701080"/>
    <dbReference type="KEGG" id="nph:NP_1190A"/>
    <dbReference type="eggNOG" id="arCOG09213">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2893115_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055736">
        <property type="entry name" value="DUF7312"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23994">
        <property type="entry name" value="DUF7312"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7312" evidence="3">
        <location>
            <begin position="9"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="70" mass="7935" checksum="304DF80650E3E56F" modified="2017-05-10" version="1">MSDSEEPPDEEWAYTLEDIEEREAEAEADAERERRQTEPIAAGDPSLENVFFVLLGVGFTLFIISRLFVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUN6</accession>
    <name>A0A1U7EUN6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Mechanosensitive channel protein MscS</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">mscS1</name>
        <name evidence="11" type="ordered locus">NP_1184A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
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        <property type="protein sequence ID" value="CAI48683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUN6"/>
    <dbReference type="STRING" id="348780.NP_1184A"/>
    <dbReference type="EnsemblBacteria" id="CAI48683">
        <property type="protein sequence ID" value="CAI48683"/>
        <property type="gene ID" value="NP_1184A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702938"/>
    <dbReference type="KEGG" id="nph:NP_1184A"/>
    <dbReference type="eggNOG" id="arCOG01568">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037945_0_3_2"/>
    <dbReference type="OrthoDB" id="31543at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049278">
        <property type="entry name" value="MS_channel_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045275">
        <property type="entry name" value="MscS_archaea/bacteria_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_beta-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011066">
        <property type="entry name" value="MscS_channel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011014">
        <property type="entry name" value="MscS_channel_TM-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30221">
        <property type="entry name" value="SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30221:SF20">
        <property type="entry name" value="SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21082">
        <property type="entry name" value="MS_channel_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82689">
        <property type="entry name" value="Mechanosensitive channel protein MscS (YggB), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82861">
        <property type="entry name" value="Mechanosensitive channel protein MscS (YggB), transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="122"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="185"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS" evidence="9">
        <location>
            <begin position="207"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS C-terminal" evidence="10">
        <location>
            <begin position="279"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="365"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="390"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI48683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="402" mass="43403" checksum="98168FC830B0CA0F" modified="2017-05-10" version="1">MQLFEDARLALEGLATTEARLAVSAIIAIVAIIVATAVLPRIIRRIVDAVDHQLETADEESFATAVDEAVQLPFPTRLVVRTLQTGITVVVALVLLIVWGYLDIARLVAAVLADALPILGRLLLSIGLLAAGIVGTRVLETELESMTADSEFVNQHQQGVLYRVLQITVFTAVGLAALSLWDFNLGGLLVGAGFLGIVVGMAARQTLGSMIAGFVLMFSRPFEIGDWVVIGDEEGIVTDITIINTRLRSFDGEEIVMPNDAVSDGTVINRTRRGRLRLRTEVGIDYEADIERAEQLAAEAMASVDEVAPGPKPQVVPTAFGDSAVVLELRYWIRDPSARKKWQTKQAVVRTVKQRFDDAGVDIPYPHRTLDSRPGFAVSGATNASAPSPEARDSDGTPSREE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUQ4</accession>
    <name>A0A1U7EUQ4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC-type transport system permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">abc26p</name>
        <name evidence="2" type="ordered locus">NP_1200A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322327.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUQ4"/>
    <dbReference type="STRING" id="348780.NP_1200A"/>
    <dbReference type="EnsemblBacteria" id="CAI48691">
        <property type="protein sequence ID" value="CAI48691"/>
        <property type="gene ID" value="NP_1200A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702446"/>
    <dbReference type="KEGG" id="nph:NP_1200A"/>
    <dbReference type="eggNOG" id="arCOG02440">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071765_0_1_2"/>
    <dbReference type="OrthoDB" id="86287at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43471">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43471:SF1">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE PROTEIN NOSY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12679">
        <property type="entry name" value="ABC2_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="241"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="266" mass="27990" checksum="64E99303562173F4" modified="2017-05-10" version="1">MKRSLRTLAERDFRDPFRSRSAWPTLVLFAAVFGLFVYALGPSPGPSRPEFGQLLVAIVYFLVPLAALQFGYDRLSGPRQTGEVRLLLAPPHSRRDLLLGTALGRSAFTALVIVVGYLAAAAVFVVTVGLPAAMLTLGGLAAALGLGVAMTAIAVGASAATATTARSGMVAIGAYFLFLLWDMVLFGLRFALNGFSLPEGPQPAWAELLAELSPMEAYMNLNASLFSGEVAPDVVTASPPVAVALLVAWTVAPLLVGYARLRAVDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUR3</accession>
    <name>A0A1U7EUR3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable oxidoreductase (Short-chain dehydrogenase family)</fullName>
            <ecNumber evidence="2">1.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1294A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322373.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUR3"/>
    <dbReference type="STRING" id="348780.NP_1294A"/>
    <dbReference type="EnsemblBacteria" id="CAI48738">
        <property type="protein sequence ID" value="CAI48738"/>
        <property type="gene ID" value="NP_1294A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701105"/>
    <dbReference type="KEGG" id="nph:NP_1294A"/>
    <dbReference type="eggNOG" id="arCOG01259">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_2_2"/>
    <dbReference type="OrthoDB" id="35501at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050259">
        <property type="entry name" value="SDR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42879">
        <property type="entry name" value="3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42879:SF6">
        <property type="entry name" value="NADPH-DEPENDENT REDUCTASE BACG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="262" mass="27592" checksum="419BE92E456B875A" modified="2017-05-10" version="1">MDLDIDGNAALVTAGTAGLGLASAEALARNGANVAVCGRDEDRLSRAEAKLEAIDGGDVLAVQADITDREALQGFVGKTIETFDGLDHVVTSAGGPPSGSFLDTDDEDWYDAYDLLVMSVVRTVRSALPTLAEDGGGTVVNITSRSVQEVMDDLVLSNSVRRSVIGLMKSLSHELGPEVRVNAVLPGAHETSRIEELVEDAVERGDYDDYEAGLDDWSEGVPLERVGDPRELGETVAWLSSERASYVSGATLPVDGGSTRSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUU5</accession>
    <name>A0A1U7EUU5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NMD3 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_1358A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322405.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUU5"/>
    <dbReference type="STRING" id="348780.NP_1358A"/>
    <dbReference type="EnsemblBacteria" id="CAI48770">
        <property type="protein sequence ID" value="CAI48770"/>
        <property type="gene ID" value="NP_1358A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701125"/>
    <dbReference type="KEGG" id="nph:NP_1358A"/>
    <dbReference type="eggNOG" id="arCOG04149">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065087_0_0_2"/>
    <dbReference type="OrthoDB" id="15051at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039768">
        <property type="entry name" value="Nmd3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007064">
        <property type="entry name" value="Nmd3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12746:SF2">
        <property type="entry name" value="60S RIBOSOMAL EXPORT PROTEIN NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12746">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04981">
        <property type="entry name" value="NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nmd3 N-terminal" evidence="2">
        <location>
            <begin position="11"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="374" mass="40769" checksum="92E11C43DBD3F806" modified="2017-05-10" version="1">MSSGSRSGEFCPRCGDEISRPSDIDLPSGPSDPDAVLCDGCYFEDFDLVDAPERIEVRVCSQCGALHRGNRWVDVGARDYTDIAVEETADRLAVHVDAREVDWGVEPEQVDQNTIRMHCLFSGVIRGTAVSEEVTVPVYVARETCDRCGRIAGDYYASIIQVRGTDRTPTTDENDRAVEIAESYIAEREATGDRNAFITETSRTDDGVDMKISTNQMGQGIAKRIVAELGGTVSEAPTLVTEDGDGNEVYRVTFTARLPPYTPGDVIDLDDGDGPVLVTSAHGNLKGVRLATGDRYEADFEEGIDPDARTLGTREDAESTTLVAVEDAHAVQVLDPETFESKTVPRPSYLDPDADEVPVLKHRQGLHVLPDDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUZ0</accession>
    <name>A0A1U7EUZ0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">GNAT family acetyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_1474A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322463.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUZ0"/>
    <dbReference type="STRING" id="348780.NP_1474A"/>
    <dbReference type="EnsemblBacteria" id="CAI48828">
        <property type="protein sequence ID" value="CAI48828"/>
        <property type="gene ID" value="NP_1474A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701157"/>
    <dbReference type="KEGG" id="nph:NP_1474A"/>
    <dbReference type="eggNOG" id="arCOG00840">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1387553_0_0_2"/>
    <dbReference type="OrthoDB" id="104811at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="193" mass="21688" checksum="146ED2D51F22155E" modified="2017-05-10" version="1">MYVRDARNRDEAWLLDHIEAMGLDETSFRSRDYVIAVDEESNARAGFGRIRVHSDDGTEVCELTSIGVLDGWRRQGVGAHVIERLVDSAETEAFERVYSLTSAHDYLAQFGFEPVAEAELPDVLAERLEAKQDTLDPDAVPMRLDIDAFGMPERLRERFKTAAEDAEPAEPPEEAEDFGIDPDEATYKYDTGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUZ3</accession>
    <name>A0A1U7EUZ3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Molybdopterin molybdenumtransferase</fullName>
            <ecNumber evidence="4">2.10.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">moeA1</name>
        <name evidence="4" type="ordered locus">NP_1440A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </dbReference>
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        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
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            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI48811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="402" mass="42715" checksum="AF93F101598C46CF" modified="2017-05-10" version="1">MADVRESGFKERTRVAAAREQLLDIPPIDRTERISLSAADGRALAAPVAARRNIPHYARAAMDGWAVRAEDTFGASDRSPAVLRPGDSVGPNTAVRVHTGSELPEGADAVVMVEATEQVGDELEVFDAVGAGENVGPIGEDVEDGQRLYEPGHQLRPSDLGLLKGAGIDTVEVAARPEVAVIPTGEELVQSDPDPGEIVETNRFTVSRLAERWGADVTRRSIVTDDREALRAAVERDLTRDVVVTTGGSSVGERDLIPEVVSDLGEVFVHGVALKPGHPVALGRVNGVTIVMLPGYPVAAIVNAVQFLRPLIKHLGRFPSEPLPSQAATLTRKIRSEPGVRTFARVTLSPTDDGLEATPTRASGSGVLSSVALADGWVVVPEQREGIPADETVAVQDWEHNV</sequence>
</entry>
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    <accession>A0A1U7EUZ9</accession>
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        <submittedName>
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        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">NP_1438A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <dbReference type="NCBI Taxonomy" id="348780"/>
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            <taxon>Halobacteria</taxon>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
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            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018746">
        <property type="entry name" value="DUF2298"/>
    </dbReference>
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        <property type="entry name" value="Chlor_Arch_YYY"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10790">
        <property type="entry name" value="TPR-DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10060">
        <property type="entry name" value="DUF2298"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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        </location>
    </feature>
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        <location>
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        </location>
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        <location>
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        <location>
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        </location>
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        <location>
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        </location>
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        <location>
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            <end position="402"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="587"/>
            <end position="606"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI48810.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="764" mass="80869" checksum="E15D6C009F5FECC8" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV09</accession>
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    <protein>
        <submittedName>
            <fullName evidence="1">HTH domain protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ordered locus">NP_1460A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
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            <taxon>Halobacteriales</taxon>
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        </lineage>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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            <authorList>
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                <person name="Pfeiffer F."/>
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                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
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            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
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        <property type="gene ID" value="NP_1460A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701156"/>
    <dbReference type="KEGG" id="nph:NP_1460A"/>
    <dbReference type="eggNOG" id="arCOG06237">
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    </dbReference>
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    <dbReference type="OrthoDB" id="165520at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="173" mass="20569" checksum="5992D7A996FF5B7C" modified="2017-05-10" version="1">MTSSDAPDWDFKERDVLVLRELSRDPQLSSRDLARILEEKYDIDVSHVTVSESIRGMREEGVFREAVIPNEEYFHFALFEFKFNPENFDDGWRDAMEFIRDSPNTLFYFLSDGEYQWKSVMMFSDREAQSQWIHQFYKEHGDIVHNLRNSVVTNVLKFRTDPEVLSSLHEGDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV35</accession>
    <name>A0A1U7EV35_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1558A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
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            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV35"/>
    <dbReference type="STRING" id="348780.NP_1558A"/>
    <dbReference type="EnsemblBacteria" id="CAI48870">
        <property type="protein sequence ID" value="CAI48870"/>
        <property type="gene ID" value="NP_1558A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_1558A"/>
    <dbReference type="HOGENOM" id="CLU_3338561_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="37" mass="4180" checksum="DC0A9806ED62CC07" modified="2017-05-10" version="1">MEETVVQKSHRRLLSDGGGRQTTDIYRPRPDTGLHTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV72</accession>
    <name>A0A1U7EV72_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1652A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322551.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV72"/>
    <dbReference type="STRING" id="348780.NP_1652A"/>
    <dbReference type="EnsemblBacteria" id="CAI48917">
        <property type="protein sequence ID" value="CAI48917"/>
        <property type="gene ID" value="NP_1652A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701212"/>
    <dbReference type="KEGG" id="nph:NP_1652A"/>
    <dbReference type="eggNOG" id="arCOG11365">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1451446_0_0_2"/>
    <dbReference type="OrthoDB" id="242689at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="177" mass="18446" checksum="4DCD484CCDC9C2AA" modified="2017-05-10" version="1">MTRKLSRRQLVSGLAAAGAGVAAGCTDGEEGDSEDEEDTDIDPGLAVGDRALSSAFPLELYEPGTDERVATVHWHGPEFSHWHFGPLEIPAGDSRTLQAQFNADGGGTIDIGEGEPYAVSVGRTEATPEELLSVSVAGDEITVSGGESGEGELVFQLRHEGDVVWVSPPLSTDVAGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV89</accession>
    <name>A0A1U7EV89_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1672A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322560.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV89"/>
    <dbReference type="STRING" id="348780.NP_1672A"/>
    <dbReference type="EnsemblBacteria" id="CAI48927">
        <property type="protein sequence ID" value="CAI48927"/>
        <property type="gene ID" value="NP_1672A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701218"/>
    <dbReference type="KEGG" id="nph:NP_1672A"/>
    <dbReference type="eggNOG" id="arCOG04616">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2839256_0_0_2"/>
    <dbReference type="OrthoDB" id="311563at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043823">
        <property type="entry name" value="DUF5800"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19115">
        <property type="entry name" value="DUF5800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="65" mass="7464" checksum="A01C644DC99E618A" modified="2017-05-10" version="1">MTVLSFDEEGVDVIYEGTEFRLEKDLIEEATEKPYPEVTDHEVLQIIEENPSLSDEPRRIADILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVA2</accession>
    <name>A0A1U7EVA2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UspA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_1718A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322583.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVA2"/>
    <dbReference type="STRING" id="348780.NP_1718A"/>
    <dbReference type="EnsemblBacteria" id="CAI48950">
        <property type="protein sequence ID" value="CAI48950"/>
        <property type="gene ID" value="NP_1718A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701236"/>
    <dbReference type="KEGG" id="nph:NP_1718A"/>
    <dbReference type="eggNOG" id="arCOG04802">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121761_0_0_2"/>
    <dbReference type="OrthoDB" id="312762at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF6">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN UP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="4"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="144" mass="14968" checksum="41B3B29B8F22EAE4" modified="2017-05-10" version="1">MDVDTVLVPVDGSETAMDAVEYAVEIADRYGAGVHALYLVGENAAAEMEAGAVDATDIATRGEQIMASVRAIAGTVPVDHSSACGFSTCRLSQHPGSVILDVAEMLAVDFVVVPRQPVTGDEEAVIEKAAEYVIAYASQPVLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVQ1</accession>
    <name>A0A1U7EVQ1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Fla cluster protein FlaF</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">flaF</name>
        <name evidence="1" type="ordered locus">NP_2094A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322766.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVQ1"/>
    <dbReference type="STRING" id="348780.NP_2094A"/>
    <dbReference type="EnsemblBacteria" id="CAI49138">
        <property type="protein sequence ID" value="CAI49138"/>
        <property type="gene ID" value="NP_2094A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702711"/>
    <dbReference type="KEGG" id="nph:NP_2094A"/>
    <dbReference type="eggNOG" id="arCOG01824">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145174_0_0_2"/>
    <dbReference type="OrthoDB" id="62189at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097588">
        <property type="term" value="P:archaeal or bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002774">
        <property type="entry name" value="Flagellin_arc-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42200:SF2">
        <property type="entry name" value="ARCHAEAL FLAGELLA-RELATED PROTEIN F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42200">
        <property type="entry name" value="ARCHAEAL FLAGELLA-RELATED PROTEIN F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01917">
        <property type="entry name" value="Flagellin_arch-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="151" mass="15533" checksum="B844D7025B0DBDD1" modified="2017-05-10" version="1">MGFSVSGSAVIVFIGLVVAAGIAVPPLMGSVGNLASAQGTQIDQGTERLNTDVEIAAATYDDDAEELDIELENTGTTSLSVADTDLLVDGAMQTDADNLATTVEGDADAELWLPAETLTMTVGATTEPDRVKVVTENGIERATNDITDGET</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVR5</accession>
    <name>A0A1U7EVR5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UbiB family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2118A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322778.1">
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        <property type="gene ID" value="NP_2118A"/>
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    <dbReference type="KEGG" id="nph:NP_2118A"/>
    <dbReference type="eggNOG" id="arCOG01189">
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    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="F:protein kinase activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="ABC1_dom"/>
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        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
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        <property type="entry name" value="UbiB_kinase"/>
    </dbReference>
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        <property type="entry name" value="CHAPERONE-ACTIVITY OF BC1 COMPLEX CABC1 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10566:SF113">
        <property type="entry name" value="PROTEIN ACTIVITY OF BC1 COMPLEX KINASE 7, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="112"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="516" mass="57277" checksum="ADF4E4047F71DEC9" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVS1</accession>
    <name>A0A1U7EVS1_NATPD</name>
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        <submittedName>
            <fullName evidence="2">Probable oxidoreductase (Homolog to saccharopine dehydrogenase)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2112A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="348780.NP_2112A"/>
    <dbReference type="EnsemblBacteria" id="CAI49147">
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        <property type="gene ID" value="NP_2112A"/>
    </dbReference>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046808_0_0_2"/>
    <dbReference type="OrthoDB" id="194971at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Sacchrp_dh_NADP-bd"/>
    </dbReference>
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        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43781:SF1">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="4"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="343" mass="36728" checksum="9430E8D7A53802E1" modified="2017-05-10" version="1">MSLLIYGAYGYTGELIAEEAVDRDLDVVVAGRDKRKVRDLAARLNCKGRRFDLDAAADNLDDIEAVLNCAGPFVETYEPLVEACLETGTHYLDITGELSVFEAIAERDREAEKAGVCLLPGVGFDVVPTDCLAGHLHDMHPSADELRLGFDPSGTISKGTLASVIEHLDEGGKVRRDGRLVDVPPAHKRRYIDFGRGERSAATIPWGDVSTAYYTTGIENIEVYTAMPSEAAIALKYGSGIVSPVLGAEPVKGALQTLAKTVVSGPSEKQRREGVCYVWGEATDGERTVTARLKTPETYALTVDAATTAAARILDDAPAGFETPAAAFTPEFVLALDGVEGFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVT4</accession>
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        <recommendedName>
            <fullName evidence="4">Flagella cluster protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="2" type="ordered locus">NP_2100A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322769.1">
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVT4"/>
    <dbReference type="STRING" id="348780.NP_2100A"/>
    <dbReference type="EnsemblBacteria" id="CAI49141">
        <property type="protein sequence ID" value="CAI49141"/>
        <property type="gene ID" value="NP_2100A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701349"/>
    <dbReference type="KEGG" id="nph:NP_2100A"/>
    <dbReference type="eggNOG" id="arCOG04698">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086563_0_0_2"/>
    <dbReference type="OrthoDB" id="177137at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055923">
        <property type="entry name" value="DUF7500"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24332">
        <property type="entry name" value="DUF7500"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="230" mass="25487" checksum="258EF1B55BF1A5FD" modified="2017-05-10" version="1">MPDDIPPEFPDDDGDDSDDDYVDPDVPNEKGGGDGGKVLSPDELDLSDSEYVEELDDQGRYVVSPGSGPPNVPDRGSEDDRQGRRERRDRGGPQHREERPQQDRGGRQREPAPNSTPVSPEAARSLLADELERTDSRYGLDIVARFDGTTVRHRTASDDVIATFENLVQWYAQHVTDDTPADEVIDILLRESALASTETPNLAKLLRKHDLDTSDSIAELVDAIRDEAQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVZ0</accession>
    <name>A0A1U7EVZ0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">NADH dehydrogenase-like complex subunit L</fullName>
            <ecNumber evidence="8">1.6.5.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">nuoL</name>
        <name evidence="8" type="ordered locus">NP_2310A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.6.5.-" evidence="8"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322873.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVZ0"/>
    <dbReference type="STRING" id="348780.NP_2310A"/>
    <dbReference type="EnsemblBacteria" id="CAI49246">
        <property type="protein sequence ID" value="CAI49246"/>
        <property type="gene ID" value="NP_2310A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702997"/>
    <dbReference type="KEGG" id="nph:NP_2310A"/>
    <dbReference type="eggNOG" id="arCOG01539">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007100_6_0_2"/>
    <dbReference type="OrthoDB" id="371891at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018393">
        <property type="entry name" value="NADHpl_OxRdtase_5_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003945">
        <property type="entry name" value="NU5C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001516">
        <property type="entry name" value="Proton_antipo_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01974">
        <property type="entry name" value="NDH_I_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005141">
        <property type="entry name" value="PRK06590.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42829">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42829:SF2">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00662">
        <property type="entry name" value="Proton_antipo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01434">
        <property type="entry name" value="NADHDHGNASE5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01435">
        <property type="entry name" value="NPOXDRDTASE5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="33"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="121"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="184"/>
            <end position="205"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="144" mass="15996" checksum="7399B9B2D442B827" modified="2017-05-10" version="1">MRKPRVTDDETHPAADIDAVVADPEDVAELLHREHTESDPLRSHGFRIDPPLEGDVRAEPHVQDGPVRHQGQAPQPIHLPPATFVANESGDHPNETHLEPPTEAPDEEWRSAVEEALISPVRVSFDDRTGNEVWVTARYESGDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW56</accession>
    <name>A0A1U7EW56_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2428A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322931.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW56"/>
    <dbReference type="STRING" id="348780.NP_2428A"/>
    <dbReference type="EnsemblBacteria" id="CAI49305">
        <property type="protein sequence ID" value="CAI49305"/>
        <property type="gene ID" value="NP_2428A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701441"/>
    <dbReference type="KEGG" id="nph:NP_2428A"/>
    <dbReference type="eggNOG" id="arCOG02298">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1567083_0_0_2"/>
    <dbReference type="OrthoDB" id="142616at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.820.10">
        <property type="entry name" value="Structural Genomics, Unknown Function 30-nov-00 1gh9 Mol_id"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043855">
        <property type="entry name" value="DUF5817"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053849">
        <property type="entry name" value="DUF5817_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19134">
        <property type="entry name" value="DUF5817"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22798">
        <property type="entry name" value="DUF5817_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5817" evidence="1">
        <location>
            <begin position="2"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="DUF5817" evidence="2">
        <location>
            <begin position="112"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="165" mass="17747" checksum="D833775A0439CCA7" modified="2017-05-10" version="1">MYTVVGCPACRAIWVVEDRPETTECRRCGKRHRFGKLRSFAQTDTADAAARVRSSMLADRADDGAFVDPQTIDIEGVGIDDATFLSASGVDPDAVADAGETDTSGSRSRKRILLDGLSELDAPTEAEVVEYATAAGVPEAKAERLLTKLRRAGEVTETDGTLRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW60</accession>
    <name>A0A1U7EW60_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Probable COG0212-type thiamine metabolism protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2438A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322936.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW60"/>
    <dbReference type="STRING" id="348780.NP_2438A"/>
    <dbReference type="EnsemblBacteria" id="CAI49310">
        <property type="protein sequence ID" value="CAI49310"/>
        <property type="gene ID" value="NP_2438A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701445"/>
    <dbReference type="KEGG" id="nph:NP_2438A"/>
    <dbReference type="eggNOG" id="arCOG00474">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031500_2_0_2"/>
    <dbReference type="OrthoDB" id="18307at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10420">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002698">
        <property type="entry name" value="FTHF_cligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024185">
        <property type="entry name" value="FTHF_cligase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13017">
        <property type="entry name" value="5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13017:SF0">
        <property type="entry name" value="METHENYLTETRAHYDROFOLATE SYNTHASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01812">
        <property type="entry name" value="5-FTHF_cyc-lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="235" mass="25427" checksum="D1512AB6B86689B9" modified="2017-05-10" version="1">MSATDKETLRSRIWDRLESDGLARFPFPPHGRITNFDGAAEAAQRAMDLPQLADAGAVKANPDAPQLPLRRRLLKDGTTVYMAVPRLSEPACFVELDPESLSDLDAAPTVSHMRNYGRPVEPPSMPSIDAIVVGSVAVTRDGVRVGKGEGYSDLEYAVLREYGLVDDGTPVITTVHDVQVVDETVAAAAHDVPIDIVCTPTETIRLDSGSKPDGIDWDALDDDRREAIPVLESLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW80</accession>
    <name>A0A1U7EW80_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DUF1611 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2482A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322958.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW80"/>
    <dbReference type="STRING" id="348780.NP_2482A"/>
    <dbReference type="EnsemblBacteria" id="CAI49332">
        <property type="protein sequence ID" value="CAI49332"/>
        <property type="gene ID" value="NP_2482A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701459"/>
    <dbReference type="KEGG" id="nph:NP_2482A"/>
    <dbReference type="eggNOG" id="arCOG02828">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059741_0_0_2"/>
    <dbReference type="OrthoDB" id="30617at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011669">
        <property type="entry name" value="DgcN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035086">
        <property type="entry name" value="DgcN-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035402">
        <property type="entry name" value="DgcN-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40690:SF1">
        <property type="entry name" value="DUF1611 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40690">
        <property type="entry name" value="GLL3100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07755">
        <property type="entry name" value="DUF1611"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17396">
        <property type="entry name" value="DUF1611_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF026760">
        <property type="entry name" value="UCP026760"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-glutamate N-acetyltransferase-like N-terminal" evidence="2">
        <location>
            <begin position="35"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="D-glutamate N-acetyltransferase-like C-terminal" evidence="1">
        <location>
            <begin position="134"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="336" mass="35313" checksum="16802627392D7160" modified="2017-05-10" version="1">MRVAILAHEQFPGRAKTAVGVLRYGTDDVVAVLDRERAGTAVGDHLDDIDAEIPVVASAADAPDFDALYIGIAPIGGGFDASWRPDVRTAIERGADVVSGLHYFLSDDQEFTSLADEYGVELRDVRQPPADLTVADGIARDLDAEIVLTVGTDCSTGKMTTTLELVEAAREAGLDAGFVPTGQTGIMIAGDGIPIDRTISDFTAGAAERLVATAAEDHDYLFVEGQGSILHPAYSPVTCGLLHGTQPDWLVLCHEAGRKRVHGYEDFAIPEPSAVADQYLSLAEPVAPTTLLGGALNTSHLDGGAAETAIDDYADELGVPVVDPVRQSAAPIVEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWE3</accession>
    <name>A0A1U7EWE3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">3-hydroxyacyl-CoA dehydrogenase</fullName>
            <ecNumber evidence="6">1.1.1.35</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">hbd1</name>
        <name evidence="6" type="ordered locus">NP_2630A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.35" evidence="6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323031.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWE3"/>
    <dbReference type="STRING" id="348780.NP_2630A"/>
    <dbReference type="EnsemblBacteria" id="CAI49406">
        <property type="protein sequence ID" value="CAI49406"/>
        <property type="gene ID" value="NP_2630A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702792"/>
    <dbReference type="KEGG" id="nph:NP_2630A"/>
    <dbReference type="eggNOG" id="arCOG00250">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_2_0_2"/>
    <dbReference type="OrthoDB" id="51300at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003857">
        <property type="term" value="F:(3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000009">
        <property type="entry name" value="Fatty oxidation complex, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022694">
        <property type="entry name" value="3-OHacyl-CoA_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006176">
        <property type="entry name" value="3-OHacyl-CoA_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006108">
        <property type="entry name" value="3HC_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48075">
        <property type="entry name" value="3-HYDROXYACYL-COA DEHYDROGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48075:SF5">
        <property type="entry name" value="3-HYDROXYBUTYRYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00725">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02737">
        <property type="entry name" value="3HCDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000105">
        <property type="entry name" value="HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="1 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase NAD binding" evidence="5">
        <location>
            <begin position="2"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase C-terminal" evidence="4">
        <location>
            <begin position="184"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="7"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="2">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000105-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000105-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI49406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="295" mass="32078" checksum="1ED7C01108598125" modified="2017-05-10" version="1">MQVTVLGAGSMGHGIAQSSAMAGYDVVLRDIEAERVENGIEEIRQNLEGGVERDKVSEAEMEATLDRIEGETDLAAAVSDAALVIEAVPEDMELKKDVLSDVEAETDAETVLASNTSSLSVTEMASALEHPERVVGLHFFNPPHLMDLVEIVIAEQTDDRAESFAVDYVREIDKEDIVVRDSAGFATSRLGLVTGLEAIRMVEEGVASPADIDEAMKLGYGYPMGPIELGDHVGLDVRLHIAEHLREELGERFRPPQALRQKVRAGKLGKKSGEGFYVWEDGERVGMSGDWGDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWJ6</accession>
    <name>A0A1U7EWJ6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Receiver box response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">NP_2716A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323074.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWJ6"/>
    <dbReference type="STRING" id="348780.NP_2716A"/>
    <dbReference type="EnsemblBacteria" id="CAI49449">
        <property type="protein sequence ID" value="CAI49449"/>
        <property type="gene ID" value="NP_2716A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701534"/>
    <dbReference type="KEGG" id="nph:NP_2716A"/>
    <dbReference type="eggNOG" id="arCOG02387">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02389">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_835788_0_0_2"/>
    <dbReference type="OrthoDB" id="8127at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00156">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050595">
        <property type="entry name" value="Bact_response_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44591:SF25">
        <property type="entry name" value="CHEMOTAXIS TWO-COMPONENT RESPONSE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44591">
        <property type="entry name" value="STRESS RESPONSE REGULATOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="6"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="4">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI49449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="340" mass="37662" checksum="8A6C3A7883FBBF44" modified="2017-05-10" version="1">MAGPVRVLLVDDDPSLADLMASQLGSLREEFSVRVETDPNDALEAVEADDIDCVVSDYHMPEMNGLELLQYVREMDPNIPFILFTARGSEEIASEAVSEGVTDYFRKRRGSEQWRVLANRIENVAARYRAEEAVQRRESALRQLARTVIDSVSTPIEELLELGRETLELEYSAVVEHDGDNGEQLVESVSDDLPFETDGEIPLADDIDGLTVDGSGIVAVATGEKAEPNEGFSAYVGTPVYVGDQRYGTLCFCDSGPRSEFTAWERAFVELLGDWLGHELTSEWARDQSESVRSAQSRLTSAREALDAGDKERVRDELEVIRELLDQNPPSSVPVSIELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWP0</accession>
    <name>A0A1U7EWP0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 14">Mevalonate kinase</fullName>
            <shortName evidence="14">MK</shortName>
            <shortName evidence="14">MVK</shortName>
            <ecNumber evidence="3 14">2.7.1.36</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14 17" type="primary">mvk</name>
        <name evidence="17" type="ordered locus">NP_2850A</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the phosphorylation of (R)-mevalonate (MVA) to (R)-mevalonate 5-phosphate (MVAP). Functions in the mevalonate (MVA) pathway leading to isopentenyl diphosphate (IPP), a key precursor for the biosynthesis of isoprenoid compounds such as archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(R)-mevalonate + ATP = (R)-5-phosphomevalonate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17065"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36464"/>
            <dbReference type="ChEBI" id="CHEBI:58146"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.36"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="13 14">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the GHMP kinase family. Mevalonate kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.36" evidence="3 14"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323141.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWP0"/>
    <dbReference type="STRING" id="348780.NP_2850A"/>
    <dbReference type="EnsemblBacteria" id="CAI49516">
        <property type="protein sequence ID" value="CAI49516"/>
        <property type="gene ID" value="NP_2850A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702960"/>
    <dbReference type="KEGG" id="nph:NP_2850A"/>
    <dbReference type="eggNOG" id="arCOG01028">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017814_0_0_2"/>
    <dbReference type="OrthoDB" id="19001at2157"/>
    <dbReference type="UniPathway" id="UPA00057">
        <property type="reaction ID" value="UER00098"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004335">
        <property type="term" value="F:galactokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004496">
        <property type="term" value="F:mevalonate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000151">
        <property type="entry name" value="Mevalonate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00217">
        <property type="entry name" value="Mevalonate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000705">
        <property type="entry name" value="Galactokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006205">
        <property type="entry name" value="Mev_gal_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022937">
        <property type="entry name" value="Mevalonate_kinase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00549">
        <property type="entry name" value="mevalon_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43290">
        <property type="entry name" value="MEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43290:SF2">
        <property type="entry name" value="MEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00473">
        <property type="entry name" value="GALCTOKINASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00959">
        <property type="entry name" value="MEVGALKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 14" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="8 14 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 14" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11 14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14 17" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="15">
        <location>
            <begin position="85"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="16">
        <location>
            <begin position="236"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="112"/>
            <end position="122"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAI49516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="329" mass="34578" checksum="5EB9EE9151ACCE98" modified="2017-05-10" version="1">MTTSSAPGKVYLFGEHAVVYGEPAVPCAIQRRARVTVEERDDDRLQVQANDLTLDGFTVTWGGETNDRPDVDVPAPLVEAAMGYIDGAIEQALDAVDVEECGFDITVESDIPLGAGLGSSAAVVVAGIDAAVRELGGELDAQAVAERAYEVEYEVQSGEASRADTFCSAVGGAVRVRGDDCRRLDDVPTLPFVVGYDGGTGDTGELVAGVRELREEYDFAADTVESIGDIVRKGEAALQRGDIEEVGRLMDFNHGLLSALGVSARSLDTMVWAAREADALGAKLTGAGGGGCIVALDRTEQTHTALEYTPGCETAFRAALDTDGVRVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWQ1</accession>
    <name>A0A1U7EWQ1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">tRNA carboxymethyluridine synthase</fullName>
            <ecNumber evidence="13">2.3.1.311</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">elp3</name>
        <name evidence="17" type="ordered locus">NP_2872A</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>uridine(34) in tRNA + acetyl-CoA + S-adenosyl-L-methionine + H2O = 5-(carboxymethyl)uridine(34) in tRNA + 5'-deoxyadenosine + L-methionine + CoA + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:61020"/>
            <dbReference type="Rhea" id="RHEA-COMP:10407"/>
            <dbReference type="Rhea" id="RHEA-COMP:11727"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74882"/>
            <dbReference type="EC" id="2.3.1.311"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:61021"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="15">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ELP3 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.311" evidence="13"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323152.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWQ1"/>
    <dbReference type="STRING" id="348780.NP_2872A"/>
    <dbReference type="EnsemblBacteria" id="CAI49527">
        <property type="protein sequence ID" value="CAI49527"/>
        <property type="gene ID" value="NP_2872A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701578"/>
    <dbReference type="KEGG" id="nph:NP_2872A"/>
    <dbReference type="eggNOG" id="arCOG01361">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025983_2_1_2"/>
    <dbReference type="OrthoDB" id="49957at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106261">
        <property type="term" value="F:tRNA uridine(34) acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002926">
        <property type="term" value="P:tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039661">
        <property type="entry name" value="ELP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034687">
        <property type="entry name" value="ELP3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056591">
        <property type="entry name" value="ELP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032432">
        <property type="entry name" value="Radical_SAM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01211">
        <property type="entry name" value="ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135:SF7">
        <property type="entry name" value="TRNA URIDINE(34) ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23613">
        <property type="entry name" value="ELP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16199">
        <property type="entry name" value="Radical_SAM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005669">
        <property type="entry name" value="Hist_AcTrfase_ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01086">
        <property type="entry name" value="elongater_protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00344">
        <property type="entry name" value="ELP3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10 15" id="KW-0408">Iron</keyword>
    <keyword evidence="11 15" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="4" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Radical SAM core" evidence="16">
        <location>
            <begin position="77"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR005669-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAI49527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="552" mass="62732" checksum="360AD6806729F3DF" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWV9</accession>
    <name>A0A1U7EWV9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2998A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323214.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWV9"/>
    <dbReference type="STRING" id="348780.NP_2998A"/>
    <dbReference type="EnsemblBacteria" id="CAI49590">
        <property type="protein sequence ID" value="CAI49590"/>
        <property type="gene ID" value="NP_2998A"/>
    </dbReference>
    <dbReference type="GeneID" id="55902677"/>
    <dbReference type="KEGG" id="nph:NP_2998A"/>
    <dbReference type="eggNOG" id="arCOG06410">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3147874_0_0_2"/>
    <dbReference type="OrthoDB" id="190451at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="48" mass="6107" checksum="8DB61F65E5C34EC9" modified="2017-05-10" version="1">MRDEEEIREQYEFLKEELDDEDMNHEGVRQMFTYYKRALGWVLEEEYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWW6</accession>
    <name>A0A1U7EWW6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3018A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWW6"/>
    <dbReference type="STRING" id="348780.NP_3018A"/>
    <dbReference type="EnsemblBacteria" id="CAI49600">
        <property type="protein sequence ID" value="CAI49600"/>
        <property type="gene ID" value="NP_3018A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3018A"/>
    <dbReference type="HOGENOM" id="CLU_2230452_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="105" mass="10698" checksum="83C48DBAD4916486" modified="2017-05-10" version="1">MCRGTAAYMPCRAVSRVISPPRTVTVTDLSGEYGQALINTLDTADGAVAYGLVFAFAVLFGSPRRSVAVWMTAGLLLWAVAFGALTAGGLSMLGTNAAEALAVAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX32</accession>
    <name>A0A1U7EX32_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">PfkB family kinase</fullName>
            <ecNumber evidence="6">2.7.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">NP_3184A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323305.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX32"/>
    <dbReference type="STRING" id="348780.NP_3184A"/>
    <dbReference type="EnsemblBacteria" id="CAI49683">
        <property type="protein sequence ID" value="CAI49683"/>
        <property type="gene ID" value="NP_3184A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703223"/>
    <dbReference type="KEGG" id="nph:NP_3184A"/>
    <dbReference type="eggNOG" id="arCOG00014">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027634_6_0_2"/>
    <dbReference type="OrthoDB" id="26949at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006796">
        <property type="term" value="P:phosphate-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002139">
        <property type="entry name" value="Ribo/fructo_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10584:SF166">
        <property type="entry name" value="RIBOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10584">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00990">
        <property type="entry name" value="RIBOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00583">
        <property type="entry name" value="PFKB_KINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="5">
        <location>
            <begin position="2"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI49683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="286" mass="30497" checksum="85040DA8ED1E0C15" modified="2017-05-10" version="1">MRVVCAGHLNWDIRLRVDRLPAPDSEGRIKDRTESGGGSAANVAVGLSSLGCAPAMLGSVGDDDRGNSARGRLERADVRQYVRTVEDAETTAKYVFTDDDGEVAMLESGGANEAFEADDLPSKALVDADMLHLTGQDPQTAAELAERAVDHDVLVSFDPGRRVDSRDYSTTLRRTDLLFVNRREAAAVDIAVPWRITKLGGDGAVCAGPEGAEISHPGFDIETVRDTTGAGDAFAAGFLAAWFEDTDPERALSVANAAGALAAGTRGVDIDLSWDRINDLRKDQPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX48</accession>
    <name>A0A1U7EX48_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3172A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49677.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939576.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX48"/>
    <dbReference type="STRING" id="348780.NP_3172A"/>
    <dbReference type="EnsemblBacteria" id="CAI49677">
        <property type="protein sequence ID" value="CAI49677"/>
        <property type="gene ID" value="NP_3172A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701675"/>
    <dbReference type="KEGG" id="nph:NP_3172A"/>
    <dbReference type="eggNOG" id="arCOG04764">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128026_0_0_2"/>
    <dbReference type="OrthoDB" id="213744at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043851">
        <property type="entry name" value="DUF5813"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19130">
        <property type="entry name" value="DUF5813"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49677.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="171" mass="19117" checksum="51C4291D7F48DE68" modified="2017-05-10" version="1">MSDELPEKAVRAFERHDAFERDGEWFTVTTTTFDGRVTAEETDDWALSYTVEVRAPMLSSATEEVVGPAVEDGWFDTYDRRLEDAPMAVRQDLELDEQRVFEEAGDAVAVFGFEWGNADHVPDMTKAIAEYVEGTYMEGIVPGYEYGQPVSEMLSQARQSAGESGRGPMPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXH4</accession>
    <name>A0A1U7EXH4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC-type transport system periplasmic substrate-binding protein (Probable substrate dipeptide/oligopeptide)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">dppA2</name>
        <name evidence="6" type="synonym">abc02s</name>
        <name evidence="6" type="ordered locus">NP_3578A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial solute-binding protein 5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXH4"/>
    <dbReference type="STRING" id="348780.NP_3578A"/>
    <dbReference type="EnsemblBacteria" id="CAI49880">
        <property type="protein sequence ID" value="CAI49880"/>
        <property type="gene ID" value="NP_3578A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3578A"/>
    <dbReference type="eggNOG" id="arCOG01534">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017028_7_4_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904680">
        <property type="term" value="F:peptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08490">
        <property type="entry name" value="PBP2_NikA_DppA_OppA_like_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.105.10">
        <property type="entry name" value="Dipeptide-binding Protein, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030678">
        <property type="entry name" value="Peptide/Ni-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PERIPLASMIC BINDING COMPONENT OF ABC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290:SF10">
        <property type="entry name" value="PERIPLASMIC OLIGOPEPTIDE-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002741">
        <property type="entry name" value="MppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Solute-binding protein family 5" evidence="5">
        <location>
            <begin position="75"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI49880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="506" mass="56242" checksum="D8AEFC27EBDADBBA" modified="2017-05-10" version="1">MKLTRRQALTAFGSAGVVGMAGCLGGGNDDTELSKTVALDSRPADGDWSMYGGLTPYYTPTHETLTASSHDFYDIEPWLATEWEAVDDLIWEFTLRTDATFHDGTALTADLVAQSFSALLAERPLGWAKVTEDSFEAVDDHTLRVETVERFGALAGTMSHPLFGIQHPGDRDDPIGTGPYEVAELDPDGAVRMVAADDYWGDEPSLEALTFEGIVDPMTRSSNLQAGDVDAAFELPREHYEMLSRDDSIEIRTQEEPRTGLGMMNLYQSPTDDADLRRALNYAVDQQLIVENILHGIGTPAKGPYAPSIPWSAHNELPEYGPDLDRARTLVDESGYDGDELEIHLGSDNPHERLIASHMQERFEEIGVSTSIRQFESGSFYEVEQKRDSNITLIELGSINGAADYLVYLQFHSEGGDNRELYETEGTGLYNLGGEVDSLIENGDRALDADRKHEAYREVQRRVMDAAVLIPVYYKEYVFGQRAETAGPETHAVPHMTRWTEFRPDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXJ3</accession>
    <name>A0A1U7EXJ3_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Multidrug-efflux transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">NP_3606A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323514.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXJ3"/>
    <dbReference type="STRING" id="348780.NP_3606A"/>
    <dbReference type="EnsemblBacteria" id="CAI49894">
        <property type="protein sequence ID" value="CAI49894"/>
        <property type="gene ID" value="NP_3606A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701821"/>
    <dbReference type="KEGG" id="nph:NP_3606A"/>
    <dbReference type="eggNOG" id="arCOG01731">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012893_5_3_2"/>
    <dbReference type="OrthoDB" id="214119at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13137">
        <property type="entry name" value="MATE_NorM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050222">
        <property type="entry name" value="MATE_MdtK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048279">
        <property type="entry name" value="MdtK-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43298:SF2">
        <property type="entry name" value="FMN_FAD EXPORTER YEEO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43298">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN NORM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006603">
        <property type="entry name" value="DinF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="113"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="149"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="188"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="346"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="373"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="410"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="436"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI49894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="477" mass="49055" checksum="802D20EE78E85B7D" modified="2017-05-10" version="1">MPGAGDDVVDVLGRVLDRLGIIDAERLRPTIELAWPRIVTGFAIMSKQTADLAMVGIAVGTAGTAGLAFALGYWSIVVLFGLGLAGGTVSLVSQNYGGGRDDRASLVVKQSALLAALFAAPVMAIFSLFGSELISILGATPEEVRHGTAYLLLIAPAVLFELLNLIASRTYTGVGDTFTEMVIRSVGAVLNLVFSALFVFGLGMGVAGVALGTTLSTGIAMVVLGWGMFGHTYGGLGMKPSPVPVSLSGPFADLTVARQLLEVAAPEIARRVAQGLTIFPLLWIAASFGPVVVTALEVARRVRALIDSVNWGMSLASSSLVGQRLGAGEETAADAYGAGIIRLTMVVYLVVAAIVVALATPIAGLFVSGSEAVAVTAAFVAVAAVSAIGLGLDGTASGVLVGAGDTRRPFVASLIGRYLFALPAAFLGLVTPLGVGGLYLALVLESFVPGGINYGLYRSGRWKVISRRYRPGTDIDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXL8</accession>
    <name>A0A1U7EXL8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Mechanosensitive channel protein MscS</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">mscS5</name>
        <name evidence="11" type="ordered locus">NP_3676A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323547.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXL8"/>
    <dbReference type="STRING" id="348780.NP_3676A"/>
    <dbReference type="EnsemblBacteria" id="CAI49929">
        <property type="protein sequence ID" value="CAI49929"/>
        <property type="gene ID" value="NP_3676A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702942"/>
    <dbReference type="KEGG" id="nph:NP_3676A"/>
    <dbReference type="eggNOG" id="arCOG01568">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037945_0_3_2"/>
    <dbReference type="OrthoDB" id="31543at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049278">
        <property type="entry name" value="MS_channel_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045275">
        <property type="entry name" value="MscS_archaea/bacteria_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_beta-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011066">
        <property type="entry name" value="MscS_channel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011014">
        <property type="entry name" value="MscS_channel_TM-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30221">
        <property type="entry name" value="SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30221:SF20">
        <property type="entry name" value="SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21082">
        <property type="entry name" value="MS_channel_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82689">
        <property type="entry name" value="Mechanosensitive channel protein MscS (YggB), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82861">
        <property type="entry name" value="Mechanosensitive channel protein MscS (YggB), transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="60"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="144"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="173"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS" evidence="9">
        <location>
            <begin position="190"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS C-terminal" evidence="10">
        <location>
            <begin position="262"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="350"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="391"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="410"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI49929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="426" mass="46776" checksum="F7552ACC4F20D231" modified="2017-05-10" version="1">MVPANSIPWYWRIIRRIQVTFDTPTAQYAATIGAVVTFVAVSLTVYYGGRRLKARRDPDAVEAMQSIAIAVTAILVSAFLVAVWRTSSEVIEALGFLSVTPESGVKALVTVIVVAVGYAVTRLTKRSIKYGSGRVTITPHQRELAHHLVQIVVLVPVLAFIIALWGIPVSNIFLGAGVLGIVLGFAARKTLSGILSGFVILFARPFEVGDWIRVGEREGIVTDITVYNTQIRTFNEEHVLVPNDTVTENEIINYSKTDRLRIETDVGVDYETDIGTAAAVAAEAMERVEAVAPQPSPDVIRLEFADSAVILRLRYWITTPTVQEKWRAQNAVIESVKQAFEDEGIKIPFPQRELTGRAETGGFHITNTPTDNDDSRKAVRRRPGAGGDISEPVEDDLADSDDEEKHPAADFEESVTDPPADEEARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXN6</accession>
    <name>A0A1U7EXN6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">NP_3752A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49967.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXN6"/>
    <dbReference type="STRING" id="348780.NP_3752A"/>
    <dbReference type="EnsemblBacteria" id="CAI49967">
        <property type="protein sequence ID" value="CAI49967"/>
        <property type="gene ID" value="NP_3752A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3752A"/>
    <dbReference type="eggNOG" id="arCOG02333">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_58_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00075">
        <property type="entry name" value="HATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050736">
        <property type="entry name" value="Sensor_HK_Regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43711:SF1">
        <property type="entry name" value="HISTIDINE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43711">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="PAS" evidence="8">
        <location>
            <begin position="16"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="9">
        <location>
            <begin position="88"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="149"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI49967.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="341" mass="37890" checksum="5B9F051E64249420" modified="2017-05-10" version="1">MRPRLSTARIGPMSRDPRRLETLFEHAPDMMNLHDAEGTILDVNERFCAVLGREREDVLGKKIWEIDTEIEPDGIQQKLGRIEPGERIAVETRFERDDGTTFPVELNVRCVDVDDERLFVVISRDISERKRREQRLQRQNERLEAFASTVSHDLRNPLATAHGYLDLARESGDPEDFDRIDQSLTRMERIIDDVLWLAREGQEIGSTSDVELATAVADAWTVVGGAGGDARIEIEDGLGTVTADSDRLRQLIENLLGNAVDHGGEVVRVEPTADGFAIEDDGPGIPESERERVFERGHTLSEDGSGLGLAIVDEIAEAHGWSVEITDGDLGGARFEVTTDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXQ5</accession>
    <name>A0A1U7EXQ5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DUF1119 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">NP_3808A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323612.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXQ5"/>
    <dbReference type="STRING" id="348780.NP_3808A"/>
    <dbReference type="EnsemblBacteria" id="CAI49995">
        <property type="protein sequence ID" value="CAI49995"/>
        <property type="gene ID" value="NP_3808A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701871"/>
    <dbReference type="KEGG" id="nph:NP_3808A"/>
    <dbReference type="eggNOG" id="arCOG04463">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053464_0_0_2"/>
    <dbReference type="OrthoDB" id="241093at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042500">
        <property type="term" value="F:aspartic endopeptidase activity, intramembrane cleaving"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006639">
        <property type="entry name" value="Preselin/SPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010545">
        <property type="entry name" value="SPP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041679">
        <property type="entry name" value="IMP_arch_presen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06550">
        <property type="entry name" value="SPP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00730">
        <property type="entry name" value="PSN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="95"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="272"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="305"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="341"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="201"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="201"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="237"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="371" mass="37865" checksum="A0B6073AD39B6BD1" modified="2017-05-10" version="1">MKRAFAAVAATVCLFLLVQLGSLALVEPFMSAGFQAVEDPSDPTNSLLYIIAILVATGVMLAAMKFGVDRLLQGLVLFAAVFISFYVFTVVVPPVVAVAGLNLVAVALALALGVGLLVYPEWYVIDAAGIVMGAGAAGLFGISFGLLPALVLLVVLAVYDAISVYGTEHMLALASGAMDLRLPVIFVVPLTLSYSFLDADNPESLEDDTPESDAEAEPDEGDDVSTATGDAEPVDTMAEDADTDADSDADADDPAAAEAAPDEHADPFARDAFFIGLGDAVIPTILVASAAFFRPGGVELLSVPGIALTVPALGAMVGTIAGLLALMWFVMKGRAHAGLPLLNGGAIAGYLLGAAAVGIPVVEALGVAPYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXR2</accession>
    <name>A0A1U7EXR2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">GNAT family acetyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3788A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323602.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXR2"/>
    <dbReference type="STRING" id="348780.NP_3788A"/>
    <dbReference type="EnsemblBacteria" id="CAI49985">
        <property type="protein sequence ID" value="CAI49985"/>
        <property type="gene ID" value="NP_3788A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701864"/>
    <dbReference type="KEGG" id="nph:NP_3788A"/>
    <dbReference type="eggNOG" id="arCOG04546">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1405952_0_0_2"/>
    <dbReference type="OrthoDB" id="213793at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="187" mass="21097" checksum="6873C3E63B97301E" modified="2017-05-10" version="1">MEPELLGWPPDGPTLRLDYRQFSYAGKFVMSSTGKAVVRDPSGADTERDFDDDIVAAVAFNADRTAPETLWLRYVTVRADRRGEGLGPRLCRFVVARAERRGYATAKIAVNNPFAYEALYRAGFAYTGETTGIAELILERPTDAPAPDRSKETYQRGLDRYRERDLEQAETAFLRRKRQATPPEPTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXS7</accession>
    <name>A0A1U7EXS7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CopG domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3834A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXS7"/>
    <dbReference type="STRING" id="348780.NP_3834A"/>
    <dbReference type="EnsemblBacteria" id="CAI50008">
        <property type="protein sequence ID" value="CAI50008"/>
        <property type="gene ID" value="NP_3834A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3834A"/>
    <dbReference type="eggNOG" id="arCOG03939">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150442_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="141" mass="16085" checksum="65C164801578E54C" modified="2017-05-10" version="1">MCKLCAVSETVMGLCARYAHRMSASDDPRRVHFQSPEYLVDRLDAIADLFDKDRTDLLVEAIREYIEDTADSETFQELVATKYYDDQLDFETVKQLVGAESAQRLRLLKKDLEDEPLDLAAPDDIDIYDGKATTVDADDER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXS8</accession>
    <name>A0A1U7EXS8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3800A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323608.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXS8"/>
    <dbReference type="EnsemblBacteria" id="CAI49991">
        <property type="protein sequence ID" value="CAI49991"/>
        <property type="gene ID" value="NP_3800A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701868"/>
    <dbReference type="KEGG" id="nph:NP_3800A"/>
    <dbReference type="eggNOG" id="arCOG06206">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_191916_0_0_2"/>
    <dbReference type="OrthoDB" id="299397at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058328">
        <property type="entry name" value="DUF8015"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26047">
        <property type="entry name" value="DUF8015"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="76" mass="7494" checksum="2789D04087E9920B" modified="2017-05-10" version="1">MTGYYDIVLGLIPASLIGLTALFVFGGLSLSASVPLASVATIGLMGHAMFVNAPGPSEPATSRPADSAAQTGVSAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXZ3</accession>
    <name>A0A1U7EXZ3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha/beta hydrolase fold protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3994A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323704.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXZ3"/>
    <dbReference type="STRING" id="348780.NP_3994A"/>
    <dbReference type="ESTHER" id="natpd-q3ip64">
        <property type="family name" value="6_AlphaBeta_hydrolase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI50088">
        <property type="protein sequence ID" value="CAI50088"/>
        <property type="gene ID" value="NP_3994A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701932"/>
    <dbReference type="KEGG" id="nph:NP_3994A"/>
    <dbReference type="eggNOG" id="arCOG01648">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_50_1_2"/>
    <dbReference type="OrthoDB" id="312142at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050228">
        <property type="entry name" value="Carboxylesterase_BioH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43194">
        <property type="entry name" value="HYDROLASE ALPHA/BETA FOLD FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43194:SF2">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN LPX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="26"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="251" mass="26903" checksum="9C4168F9D4F69EA8" modified="2017-05-10" version="1">METVSHDGRTTAYRTASDDDPTVCYIHGAGGAHDVWTQQFRGDAPPAVALDLSGHGESDDISADAGTETLDAYADDVVAVCEATGASVLCGNSMGGAVALWIVLERAFEPDGLILADTGAKLSVDPDFLDTISADFEGAVDFLHRPDMLFHEPSDELVEPSRQALLETGQDVTLRDFETCDAYDVRDRVGTVSVPTLALCGEHDPLTPPAFHEFLADELPDCSLTIFDDAAHMPMLERPAAFNEAVRSFLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY41</accession>
    <name>A0A1U7EY41_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">tRNA/rRNA cytosine-C5-methylase</fullName>
            <ecNumber evidence="8">2.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cna</name>
        <name evidence="8" type="ordered locus">NP_4072A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
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    <dbReference type="EnsemblBacteria" id="CAI50127">
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        <property type="gene ID" value="NP_4072A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702616"/>
    <dbReference type="KEGG" id="nph:NP_4072A"/>
    <dbReference type="eggNOG" id="arCOG00973">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005316_7_0_2"/>
    <dbReference type="OrthoDB" id="14725at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016428">
        <property type="term" value="F:tRNA (cytidine-5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1170">
        <property type="entry name" value="Sun protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031341">
        <property type="entry name" value="Methyltr_RsmF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011023">
        <property type="entry name" value="Nop2p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00446">
        <property type="entry name" value="nop2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807:SF74">
        <property type="entry name" value="TRNA (CYTOSINE(48)-C(5))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17125">
        <property type="entry name" value="Methyltr_RsmF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="7">
        <location>
            <begin position="17"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="231"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="107"/>
            <end position="113"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI50127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="303" mass="33114" checksum="0140C702E8D4BFD0" modified="2017-05-10" version="1">MEPLSRYDPLVDDPAAFHEACERPLPSVVRVNRIKATAESVRQAFDEAGVDYEPVDWHDGLFRLGEGESPGNSWPFVHGWVYGQEEVSAVPALALAPQPGERVLDCCAAPGSKTTQLAALMDDRGLLVGNDNNLGRLSALRSNAERCGVTNIAVTRQDARNLSLKPFGGERFDRTLVDVPCSCEGTVRKNPDAVDGWSLDHIEGIAGVQKAILERAIEVTRDGGTVVYSTCTFAPEENEAVLQHALDGGDCRLVDFDLPLESVPGVTDWEDERFDDAVRKAQRIYPHHNDTGGFFCAKLEVAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY94</accession>
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        <submittedName>
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        </submittedName>
    </protein>
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        <name evidence="1" type="ordered locus">NP_4178A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
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    <dbReference type="STRING" id="348780.NP_4178A"/>
    <dbReference type="EnsemblBacteria" id="CAI50180">
        <property type="protein sequence ID" value="CAI50180"/>
        <property type="gene ID" value="NP_4178A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701994"/>
    <dbReference type="KEGG" id="nph:NP_4178A"/>
    <dbReference type="eggNOG" id="arCOG08974">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2875182_0_0_2"/>
    <dbReference type="OrthoDB" id="316398at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="63" mass="7200" checksum="2230209F2C36A4C7" modified="2017-05-10" version="1">MNIIDGDKTECSRCEEIFPLSEVSVLERTHNKDMEKPLCEECLEAVGVPKGYEVEHDVTYLAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY98</accession>
    <name>A0A1U7EY98_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acyl-CoA synthetase</fullName>
            <ecNumber evidence="4">6.2.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">acs11</name>
        <name evidence="4" type="ordered locus">NP_4174A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
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        </lineage>
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    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
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                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY98"/>
    <dbReference type="STRING" id="348780.NP_4174A"/>
    <dbReference type="EnsemblBacteria" id="CAI50178">
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        <property type="gene ID" value="NP_4174A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702883"/>
    <dbReference type="KEGG" id="nph:NP_4174A"/>
    <dbReference type="eggNOG" id="arCOG04199">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_45_5_2"/>
    <dbReference type="OrthoDB" id="70225at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
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    <dbReference type="GO" id="GO:0016020">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05907">
        <property type="entry name" value="VL_LC_FACS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="AMP-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AMP-binding_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="3">
        <location>
            <begin position="33"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="651" mass="72786" checksum="7EAE6E7DD3D5FA33" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYB6</accession>
    <name>A0A1U7EYB6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Propionyl-CoA carboxylase carboxyltransferase component</fullName>
            <ecNumber evidence="5">6.4.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">pccB1</name>
        <name evidence="5" type="ordered locus">NP_4250A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.4.1.3" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50216.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323832.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
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        <property type="gene ID" value="NP_4250A"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="P:L-leucine catabolic process"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="AcCoA_carboxyl_beta"/>
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        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
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        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
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        <property type="entry name" value="MCCB/AccD1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22855">
        <property type="entry name" value="ACETYL, PROPIONYL, PYRUVATE, AND GLUTACONYL CARBOXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22855:SF13">
        <property type="entry name" value="METHYLCROTONOYL-COA CARBOXYLASE BETA CHAIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="3">
        <location>
            <begin position="66"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="4">
        <location>
            <begin position="322"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="36"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="307"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="498"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="58"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI50216.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="576" mass="63366" checksum="DB105A343E6058DB" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYB8</accession>
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        <submittedName>
            <fullName evidence="2">Peroxiredoxin domain protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">NP_4218A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
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            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323816.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYB8"/>
    <dbReference type="STRING" id="348780.NP_4218A"/>
    <dbReference type="EnsemblBacteria" id="CAI50200">
        <property type="protein sequence ID" value="CAI50200"/>
        <property type="gene ID" value="NP_4218A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702007"/>
    <dbReference type="KEGG" id="nph:NP_4218A"/>
    <dbReference type="eggNOG" id="arCOG00310">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1665545_0_0_2"/>
    <dbReference type="OrthoDB" id="165617at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016209">
        <property type="term" value="F:antioxidant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
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        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
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        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="3"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="158" mass="17664" checksum="F909321D7625743E" modified="2017-05-10" version="1">MPPEVGDHAPAFEALCCDGETFRPTSLTDAVGDRGAVFVFDGFVFSAIARNWWPRYEAYGWNEFDAVPVYGVVRDGPYSVNEFLRQLESPFSIFSDLNGDVAAAYDLLVERDGMVGTKTPQRAVFVLNSEGVVTERWLADDWIRPVPHEDIEAAVERL</sequence>
</entry>
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    <accession>A0A1U7EYC4</accession>
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        <submittedName>
            <fullName evidence="5">Homolog to citrate lyase beta subunit</fullName>
        </submittedName>
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        <name evidence="5" type="primary">citE</name>
        <name evidence="5" type="ordered locus">NP_4244A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323829.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYC4"/>
    <dbReference type="STRING" id="348780.NP_4244A"/>
    <dbReference type="EnsemblBacteria" id="CAI50213">
        <property type="protein sequence ID" value="CAI50213"/>
        <property type="gene ID" value="NP_4244A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702611"/>
    <dbReference type="KEGG" id="nph:NP_4244A"/>
    <dbReference type="eggNOG" id="arCOG00760">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044864_2_1_2"/>
    <dbReference type="OrthoDB" id="9170at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006107">
        <property type="term" value="P:oxaloacetate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005000">
        <property type="entry name" value="Aldolase/citrate-lyase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011206">
        <property type="entry name" value="Citrate_lyase_beta/mcl1/mcl2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32308:SF0">
        <property type="entry name" value="HPCH_HPAI ALDOLASE_CITRATE LYASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32308">
        <property type="entry name" value="LYASE BETA SUBUNIT, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G13030)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03328">
        <property type="entry name" value="HpcH_HpaI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015582">
        <property type="entry name" value="Cit_lyase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HpcH/HpaI aldolase/citrate lyase" evidence="4">
        <location>
            <begin position="4"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI50213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="279" mass="28933" checksum="799ADAE11FD62344" modified="2017-05-10" version="1">MARRSVLFAPGDSPEIMRKAAASSADVVVFDLEDAVAPGDKAEARRAVADVLADLGDGNDIEHCVRVNPVGSGAARDVAAAFAHAAPDSVMLPKAEGSDDVLRLRELLRGVDAAQPVLALVESAAGVLNADEIAAAAATDALLFGAEDLTADIGATRTDAGTEVLYARQRAVTAASAAGVDAIDTVYTDFGDTEGLRAATETAAQFGYDGKMAIHPDQVAVINEAFTPDGSDIEWAERVVTADKETDAGVFEVDGEMIDAPLVTRAERILQRAEEAGVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYH6</accession>
    <name>A0A1U7EYH6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Propionyl-CoA carboxylase small subunit</fullName>
            <ecNumber evidence="2">6.4.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pccX</name>
        <name evidence="2" type="ordered locus">NP_4366A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.4.1.3" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323890.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYH6"/>
    <dbReference type="STRING" id="348780.NP_4366A"/>
    <dbReference type="EnsemblBacteria" id="CAI50274">
        <property type="protein sequence ID" value="CAI50274"/>
        <property type="gene ID" value="NP_4366A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702041"/>
    <dbReference type="KEGG" id="nph:NP_4366A"/>
    <dbReference type="eggNOG" id="arCOG04544">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_164493_1_0_2"/>
    <dbReference type="OrthoDB" id="214756at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004658">
        <property type="term" value="F:propionyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058335">
        <property type="entry name" value="PccX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26062">
        <property type="entry name" value="DUF8022"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="80" mass="8801" checksum="26B85647F87F2EBD" modified="2017-05-10" version="1">MQVEIPPDADADEAAAIVAAVRSHVAALEAAADDGDADDDWHGDRWRFTGRLNSLQNRRSRVPTDAPRNEWAAAGRTDRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYJ3</accession>
    <name>A0A1U7EYJ3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MOSC domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4414A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323913.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYJ3"/>
    <dbReference type="STRING" id="348780.NP_4414A"/>
    <dbReference type="EnsemblBacteria" id="CAI50298">
        <property type="protein sequence ID" value="CAI50298"/>
        <property type="gene ID" value="NP_4414A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702054"/>
    <dbReference type="KEGG" id="nph:NP_4414A"/>
    <dbReference type="eggNOG" id="arCOG04766">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1080164_0_0_2"/>
    <dbReference type="OrthoDB" id="211216at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005302">
        <property type="entry name" value="MoCF_Sase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03473">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51340">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MOSC" evidence="1">
        <location>
            <begin position="112"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="317" mass="34942" checksum="F16315DE622ACEE0" modified="2017-05-10" version="1">MNADALAADHPVVSPAAADPHLQTIARFPVKSLDAEYPETARLVPSGALFGDREWAILDRPPEAPYDPTAADVGGSGDYVNGKKTAAVHRLRSTFHSREDGGPAVTLRRHDDPVSDARRFRLYDGRTGGNRAGGDAQTECHADLDAWLSSYFDRPVSVRWDGGGQHDDRTFHGPTVVSTATLREVAAWFDITVESARRRFRANLEIGGVPPFWEDRLFTDEGRAVAVRVGEATIEGYHPCQRCVVPSRDPDTGEETAGFRETFIGRRRETLPPWTDCDRFDHPFRLMVNTRVPEESAGCDIRVGDAVEILGDRPVAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYR2</accession>
    <name>A0A1U7EYR2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable secreted glycoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4616A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324014.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYR2"/>
    <dbReference type="EnsemblBacteria" id="CAI50399">
        <property type="protein sequence ID" value="CAI50399"/>
        <property type="gene ID" value="NP_4616A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702118"/>
    <dbReference type="KEGG" id="nph:NP_4616A"/>
    <dbReference type="eggNOG" id="arCOG02087">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_720833_0_0_2"/>
    <dbReference type="OrthoDB" id="163793at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="383" mass="39914" checksum="F6B820E7E37AB680" modified="2017-05-10" version="1">MNPSRRTVLKSGVGVAAAAALAGCNGNGNGNGEEDPEETPEETPDETPEETPEETPEEDPEAATVGLLADGVAYETVEREESAEFDLPVRGDFEASGEGGFVSFNEETEDEAREGGVDFPSAAEGDEAIQLEGTVAGNQWASDSIEFPVLDVNGIDVVVEAPDGVSGEIDEETGVMTLEGRFNTLIDGDEDRSFSFDISATTNQSGDLEGETTIDIDGGEVTLVDNEFVIDDQTGDGIVDANLGLPSTEPGNNWFEMLFEVDLDVVQEDEVTIDADDVAAACDAAGGDAEPVDATESADDAPTVDELGQLYDVEVPAGGGYVRFETDADALGDGNVAFFVRDGTEDVNDAETAGIASEVDCDDLDNAVVLADGETAALLQLQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYV4</accession>
    <name>A0A1U7EYV4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Dolichyl-phosphate hexosyltransferase AglJ</fullName>
            <ecNumber evidence="3">2.4.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">aglJ1</name>
        <name evidence="3" type="synonym">gtl1</name>
        <name evidence="3" type="ordered locus">NP_4658A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.4.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50420.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYV4"/>
    <dbReference type="STRING" id="348780.NP_4658A"/>
    <dbReference type="EnsemblBacteria" id="CAI50420">
        <property type="protein sequence ID" value="CAI50420"/>
        <property type="gene ID" value="NP_4658A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_4658A"/>
    <dbReference type="eggNOG" id="arCOG00894">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033536_7_3_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04179">
        <property type="entry name" value="DPM_DPG-synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026456">
        <property type="entry name" value="GCTrfase_AglJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050256">
        <property type="entry name" value="Glycosyltransferase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04182">
        <property type="entry name" value="glyco_TIGR04182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48090:SF7">
        <property type="entry name" value="RFBJ PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48090">
        <property type="entry name" value="UNDECAPRENYL-PHOSPHATE 4-DEOXY-4-FORMAMIDO-L-ARABINOSE TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="229"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="2">
        <location>
            <begin position="8"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50420.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="312" mass="33922" checksum="85AC8C8FEA6BBD84" modified="2017-05-10" version="1">MPDRTDVCVLLPTLDEAETIGDVIEGFHEEGFEHVLVVDGDSTDGTRAIAREHGARVITQSGSGKGQAVREGVEQIDQPYVLMADGDGTYRPEDADKMLTPLFAGRAEHVIGDRFADMEAGAMTRLNAVGNRLINRLFATIHGRDLQDILSGYRAFTTQSFERSQLSADGFGIETELAVECVRRGVSTAVVPIRYEARPGGSETNLHPLKDGGRILLTLYRLARMNNPIFYFGSVAAVGTAGGVALGAFVGYRYLTAGVSHEVLALLAAFLVLLSVQLLMFGVLSDIIVSIHREQSRRLDDIADQLEEQNDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYW4</accession>
    <name>A0A1U7EYW4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">VanZ family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4678A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50430.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324044.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYW4"/>
    <dbReference type="DNASU" id="3702139"/>
    <dbReference type="EnsemblBacteria" id="CAI50430">
        <property type="protein sequence ID" value="CAI50430"/>
        <property type="gene ID" value="NP_4678A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702139"/>
    <dbReference type="KEGG" id="nph:NP_4678A"/>
    <dbReference type="eggNOG" id="arCOG03232">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096028_4_0_2"/>
    <dbReference type="OrthoDB" id="214957at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037970">
        <property type="entry name" value="vanZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28008">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G10980)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28008:SF1">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G10980)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50430.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="156" mass="17145" checksum="8325164A6049426E" modified="2017-05-10" version="1">MADHSPTVKLPVPLLPWSIRWLGVLAVAGLIFYSSLATVPETVIDETDPGIVPLHLWRHILAYFGLAGVLAYATDHWAIPRWRNAVLVIAAAALYGACMELGQAFLPHRSDFLLIDVAANTLGASGVVVWYALRPYLELQPMATLAARVRGWYADR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYW6</accession>
    <name>A0A1U7EYW6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4712A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50447.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYW6"/>
    <dbReference type="STRING" id="348780.NP_4712A"/>
    <dbReference type="EnsemblBacteria" id="CAI50447">
        <property type="protein sequence ID" value="CAI50447"/>
        <property type="gene ID" value="NP_4712A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_4712A"/>
    <dbReference type="eggNOG" id="arCOG11371">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_207577_1_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055741">
        <property type="entry name" value="DUF7317"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24001">
        <property type="entry name" value="DUF7317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50447.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="57" mass="5809" checksum="A607D22280DF8237" modified="2017-05-10" version="1">MTRHAVVTGLAMYRSGAVTLEEAAACGGTSTAALAAALRCRGIELRDEDDLTVAEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYY2</accession>
    <name>A0A1U7EYY2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4736A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324072.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYY2"/>
    <dbReference type="STRING" id="348780.NP_4736A"/>
    <dbReference type="EnsemblBacteria" id="CAI50459">
        <property type="protein sequence ID" value="CAI50459"/>
        <property type="gene ID" value="NP_4736A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702162"/>
    <dbReference type="KEGG" id="nph:NP_4736A"/>
    <dbReference type="eggNOG" id="arCOG10172">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1745599_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="149" mass="16202" checksum="70D77823B9B8B801" modified="2017-05-10" version="1">MISRREFVASVTATGAVVVAGCGTDEDAAPEETPAPNDLGIELLDHTFRRTAGGGIEIELTLENRADEERSVDITVQVYDDETLLDETLVRMTTPAGEQNTSSSVLVDLAAVEAVTHYVLSVRRDPFVDPEEAPLREAFDGDRLREALE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYY9</accession>
    <name>A0A1U7EYY9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ATP-grasp fold protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_4738A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324073.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYY9"/>
    <dbReference type="STRING" id="348780.NP_4738A"/>
    <dbReference type="EnsemblBacteria" id="CAI50460">
        <property type="protein sequence ID" value="CAI50460"/>
        <property type="gene ID" value="NP_4738A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702163"/>
    <dbReference type="KEGG" id="nph:NP_4738A"/>
    <dbReference type="eggNOG" id="arCOG01589">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070819_0_0_2"/>
    <dbReference type="OrthoDB" id="345988at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053191">
        <property type="entry name" value="DcsG_Biosynth_Enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39217">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39217:SF1">
        <property type="entry name" value="GLUTATHIONE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="2">
        <location>
            <begin position="101"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="294" mass="31772" checksum="77C12D42A0C34366" modified="2017-05-10" version="1">MTDDSPAVALLTGRDHPDLTEDGRALADELKRRGIHAEPVVWSTADVDRLGDYDAALIRACWDYHTDLDGFLSFLEALSASAVAVYNPLAVVRWNAHKSYYQDLAAAGLPVPPSVCLDAGTDTSLEGILEAHGWAEAVVKPAVGAGSEGVWRTTLETAAADQQRFVAACADSDVVVQQYRPEIKHGERSLVFVDGEYSHAWNDPTKPDDFSDFEPPAFGYEPDDKTRETARAVLDVACEQAGYTPADLPYARVDYVDSDDGLLVLEVELIEPNLGLVGAGAVERLAEAVAKRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYZ4</accession>
    <name>A0A1U7EYZ4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Halocyanin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">hcp2</name>
        <name evidence="9" type="ordered locus">NP_4744A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="7">Binds 1 copper ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324076.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYZ4"/>
    <dbReference type="STRING" id="348780.NP_4744A"/>
    <dbReference type="EnsemblBacteria" id="CAI50463">
        <property type="protein sequence ID" value="CAI50463"/>
        <property type="gene ID" value="NP_4744A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702801"/>
    <dbReference type="KEGG" id="nph:NP_4744A"/>
    <dbReference type="eggNOG" id="arCOG02921">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084115_1_2_2"/>
    <dbReference type="OrthoDB" id="11088at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04220">
        <property type="entry name" value="Halocyanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002386">
        <property type="entry name" value="Amicyanin/Pseudoazurin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000923">
        <property type="entry name" value="BlueCu_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017533">
        <property type="entry name" value="Halocyanin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03102">
        <property type="entry name" value="halo_cynanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00127">
        <property type="entry name" value="Copper-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00155">
        <property type="entry name" value="AMICYANIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0186">Copper</keyword>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0574">Periplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Blue (type 1) copper" evidence="8">
        <location>
            <begin position="76"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602386-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI50463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="163" mass="17140" checksum="4B46F28045175B5C" modified="2017-05-10" version="1">MGTHTRRRFVRGTAAVAATGALAGCNGNGNGNENGNGDDAAPEDPAEAAENFLNEHDANQYDGLVDRTGEDEITIDNGAGPNGFAFDPAGVRIDAGTTVTWEWTGEGGAHNVVSEPDSDFDLETELVDEEGFTYEVTFDEPGAALYVCTAHRAQNQYGAVVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ03</accession>
    <name>A0A1U7EZ03_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Sensory rhodopsin II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">sopII</name>
        <name evidence="12" type="ordered locus">NP_4834A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="interaction">
        <interactant intactId="EBI-7339190">
            <id>A0A1U7EZ03</id>
        </interactant>
        <interactant intactId="EBI-1034515">
            <id>P42259</id>
            <label>htr2</label>
        </interactant>
        <organismsDiffer>true</organismsDiffer>
        <experiments>2</experiments>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal/bacterial/fungal opsin family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324120.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ03"/>
    <dbReference type="SMR" id="A0A1U7EZ03"/>
    <dbReference type="IntAct" id="A0A1U7EZ03">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="MINT" id="A0A1U7EZ03"/>
    <dbReference type="STRING" id="348780.NP_4834A"/>
    <dbReference type="EnsemblBacteria" id="CAI50508">
        <property type="protein sequence ID" value="CAI50508"/>
        <property type="gene ID" value="NP_4834A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703211"/>
    <dbReference type="KEGG" id="nph:NP_4834A"/>
    <dbReference type="eggNOG" id="arCOG02810">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054785_5_1_2"/>
    <dbReference type="OrthoDB" id="330248at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009881">
        <property type="term" value="F:photoreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007602">
        <property type="term" value="P:phototransduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15029">
        <property type="entry name" value="7tm_SRI_SRII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001425">
        <property type="entry name" value="Arc/bac/fun_rhodopsins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018229">
        <property type="entry name" value="Rhodopsin_retinal_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28286">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28286:SF2">
        <property type="entry name" value="BACTERIORHODOPSIN _OPSIN, NOPA (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01036">
        <property type="entry name" value="Bac_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00251">
        <property type="entry name" value="BACTRLOPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01021">
        <property type="entry name" value="Bac_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00950">
        <property type="entry name" value="BACTERIAL_OPSIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00327">
        <property type="entry name" value="BACTERIAL_OPSIN_RET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="8" id="KW-0157">Chromophore</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0681">Retinal protein</keyword>
    <keyword evidence="4" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="37"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI50508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="239" mass="25371" checksum="EE4D827F39902DD1" modified="2017-05-10" version="1">MVGLTTLFWLGAIGMLVGTLAFAWAGRDAGSGERRYYVTLVGISGIAAVAYAVMALGVGWVPVAERTVFVPRYIDWILTTPLIVYFLGLLAGLDSREFGIVITLNTVVMLAGFAGAMVPGIERYALFGMGAVAFIGLVYYLVGPMTESASQRSSGIKSLYVRLRNLTVVLWAIYPFIWLLGPPGVALLTPTVDVALIVYLDLVTKVGFGFIALDAAATLRAEHGESLAGVDTDTPAVAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ07</accession>
    <name>A0A1U7EZ07_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UspA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_4806A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324106.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ07"/>
    <dbReference type="STRING" id="348780.NP_4806A"/>
    <dbReference type="EnsemblBacteria" id="CAI50494">
        <property type="protein sequence ID" value="CAI50494"/>
        <property type="gene ID" value="NP_4806A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702182"/>
    <dbReference type="KEGG" id="nph:NP_4806A"/>
    <dbReference type="eggNOG" id="arCOG02053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_11_1_2"/>
    <dbReference type="OrthoDB" id="105697at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF6">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN UP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="143" mass="15330" checksum="023A235AB29C8FD3" modified="2017-05-10" version="1">MYDEILFPTDGSDAAEAALAHAISMADAYDAQLHVLYVVDTTYAGVGAAGNTNVNSLREEGESVLSSTADRIKDAGRPVETVLKEGHPYDEILAYSEAGIDVIVMGTRGRSGVDRYLLGSVTEKVVRTAEPPVFTVRPEEKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ79</accession>
    <name>A0A1U7EZ79_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Aldehyde ferredoxin oxidoreductase</fullName>
            <ecNumber evidence="11">1.2.7.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">aor1</name>
        <name evidence="11" type="ordered locus">NP_4964A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>tungstopterin</name>
            <dbReference type="ChEBI" id="CHEBI:30402"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AOR/FOR family.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.5" evidence="11"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <sequence length="589" mass="62331" checksum="47C838A4452371E1" modified="2017-05-10" version="1"> </sequence>
</entry>
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        </source>
    </evidence>
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</entry>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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            </authorList>
            <dbReference type="PubMed" id="16169924"/>
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        </source>
    </reference>
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    </dbReference>
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            <end position="34"/>
        </location>
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        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="64" mass="6754" checksum="DE04E8AF70827246" modified="2017-05-10" version="1">MDLTQNIQFGLLYLLTGIAMVVIDIATDIGFCLLEASGVCLVGFTTLGLIGVVIGSGLLAREYF</sequence>
</entry>
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        <recommendedName>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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                <person name="Pfeiffer F."/>
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                <person name="Rodewald K."/>
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            </authorList>
            <dbReference type="PubMed" id="16169924"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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        </source>
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        <property type="entry name" value="MJ0021-like"/>
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        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43288">
        <property type="entry name" value="BIOTIN SYNTHASE-RELATED PROTEIN, RADICAL SAM SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43288:SF1">
        <property type="entry name" value="GLYCYL-RADICAL ENZYME ACTIVATING ENZYME MJ0021-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26257">
        <property type="entry name" value="DUF8061"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01108">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="DUF8061" evidence="5">
        <location>
            <begin position="260"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF26257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI50673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="330" mass="37808" checksum="55CD205E9C2C15E1" modified="2017-05-10" version="1">MISEGCEQCAVGGKMVLFVYGYCDQRDCFYCPLGENRKNVTQVYANERPVETESDIIEEAKRMDALGSSITGGEPQEVLERTCNYISLLKDEFGEDHHTHLYTGITGGEENMRRLAEAGLDEIRFHPPLEQWGDLHGTEWEDILYTARDAGITPAFEIPGIRAEPEFLEFLDEGAAEFCNINEFEMSDGNYRRMQEAGFELREDHMSAVEGSHDILETMGDHERVYFCTSVFKDAAQHRSRLKRMARNVRREFDEITDDGTIVYGKTWVTETRLDELGVPESYYTVKSDHVELAWWLLEEMVDEGDIEDGEIVEQYPTYDGTVVERTPLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZH7</accession>
    <name>A0A1U7EZH7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11 12">Threonine synthase</fullName>
            <ecNumber evidence="4 11 12">4.2.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">thrC1</name>
        <name evidence="17" type="ordered locus">NP_5280A</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the gamma-elimination of phosphate from L-phosphohomoserine and the beta-addition of water to produce L-threonine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>O-phospho-L-homoserine + H2O = L-threonine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10840"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57590"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="EC" id="4.2.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12 13">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the threonine synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.1" evidence="4 11 12"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324340.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZH7"/>
    <dbReference type="STRING" id="348780.NP_5280A"/>
    <dbReference type="EnsemblBacteria" id="CAI50731">
        <property type="protein sequence ID" value="CAI50731"/>
        <property type="gene ID" value="NP_5280A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703253"/>
    <dbReference type="KEGG" id="nph:NP_5280A"/>
    <dbReference type="eggNOG" id="arCOG01434">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028142_0_0_2"/>
    <dbReference type="OrthoDB" id="6371at2157"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00065"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:threonine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004795">
        <property type="term" value="F:threonine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006565">
        <property type="term" value="P:L-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01563">
        <property type="entry name" value="Thr-synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000013">
        <property type="entry name" value="Threonine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050147">
        <property type="entry name" value="Ser/Thr_Dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000634">
        <property type="entry name" value="Ser/Thr_deHydtase_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004450">
        <property type="entry name" value="Thr_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026260">
        <property type="entry name" value="Thr_Synthase_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00260">
        <property type="entry name" value="thrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078:SF6">
        <property type="entry name" value="L-THREONINE DEHYDRATASE CATABOLIC TDCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038945">
        <property type="entry name" value="Thr_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00165">
        <property type="entry name" value="DEHYDRATASE_SER_THR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 12 17" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 12 13" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="16">
        <location>
            <begin position="79"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="395"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="247"/>
            <end position="251"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="386"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="14">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="NCBIfam" id="TIGR00260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR038945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR038945-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR038945-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAI50731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="419" mass="44388" checksum="2F9E3C48820F4846" modified="2017-05-10" version="1">MADLTLSPQRDAPAVSDGGVWLSCIECGHDHPPFEDIIYTCEECGGLLEARYETYPTFEDFEGHGVWRYDAALPFEEGVTLPEGATPLHRVPRLESEVGVEALRIKHEGMNPTGSFKDRGMTVGVRVAQELGVGRLACASTGNTSAALAAYGTRADLETLVLLPAGKVAAGKIAQASLHNARILEVDGNFDACLDIVQDLADRGEAYLLNSLNPFRLEGQKTIGLEILESFRDDYGRFPDRIVLPVGNAGNTAALYKCFRELVASGALEADAVPKLTGVQASGAAPMVEAVEEDLEDTRRWEDVETIATAIRIGNPVNAPKALPGIRETGGTAVAVDDDEITDAQRSLAAEGVGVEPASAASVAGLRKLRERGVVDDDEHVVCLTTGHLLKDPDAAAAAGNDPEPVANSTEAVLSHLAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZK0</accession>
    <name>A0A1U7EZK0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Homolog to rhodopsin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">NP_5296A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal/bacterial/fungal opsin family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324348.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZK0"/>
    <dbReference type="STRING" id="348780.NP_5296A"/>
    <dbReference type="EnsemblBacteria" id="CAI50739">
        <property type="protein sequence ID" value="CAI50739"/>
        <property type="gene ID" value="NP_5296A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702324"/>
    <dbReference type="KEGG" id="nph:NP_5296A"/>
    <dbReference type="eggNOG" id="arCOG02810">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1182857_0_0_2"/>
    <dbReference type="OrthoDB" id="330248at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001425">
        <property type="entry name" value="Arc/bac/fun_rhodopsins"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01036">
        <property type="entry name" value="Bac_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01021">
        <property type="entry name" value="Bac_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI50739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="218" mass="23684" checksum="CECA5301B9DC04B5" modified="2017-05-10" version="1">MIPDTTLYLVSAAIFLVSGAVFWAWTRRQPTELRKYCYPLVALVLLSAASSTLDAAGIGHLDIWAGSIELPNAVDDFIAYPVLVGFVGVLAGSSRRMLVVLVGAPVVMRTSFVVATLTEGTVSTVAGLVVVAGYLVVTAILFGPVWRTASQQNDRRRLLYWKARNLLVLLMGMLIVYSQLELLGLFDPFTGTVANQYIDLLLRIAFAGFLLSNVRSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZP4</accession>
    <name>A0A1U7EZP4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Small CPxCG-related zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4183C</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CCI69585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939658.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZP4"/>
    <dbReference type="EnsemblBacteria" id="CCI69585">
        <property type="protein sequence ID" value="CCI69585"/>
        <property type="gene ID" value="NP_4183C"/>
    </dbReference>
    <dbReference type="GeneID" id="77383468"/>
    <dbReference type="KEGG" id="nph:NP_4183C"/>
    <dbReference type="eggNOG" id="arCOG06236">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3210688_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055552">
        <property type="entry name" value="DUF7128"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23447">
        <property type="entry name" value="DUF7128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="1">
        <location>
            <begin position="13"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCI69585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="44" mass="5092" checksum="9CE2052472F04B0A" modified="2017-05-10" version="1">MVETTEKDGTTWYKCEKCGMLFDNREDATQHEASCDAEEPSYIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILS2</accession>
    <name>Q3ILS2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3242A</name>
        <name evidence="2" type="ordered locus">NP_7066A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>PL23</name>
    </geneLocation>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
            <strain evidence="2">Gabara</strain>
            <plasmid evidence="2">PL23</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CR936259">
        <property type="protein sequence ID" value="CAI50949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323334.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILS2"/>
    <dbReference type="STRING" id="348780.NP_3242A"/>
    <dbReference type="EnsemblBacteria" id="CAI49712">
        <property type="protein sequence ID" value="CAI49712"/>
        <property type="gene ID" value="NP_3242A"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI50949">
        <property type="protein sequence ID" value="CAI50949"/>
        <property type="gene ID" value="NP_7066A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703492"/>
    <dbReference type="KEGG" id="nph:NP_3242A"/>
    <dbReference type="KEGG" id="nph:NP_7066A"/>
    <dbReference type="HOGENOM" id="CLU_2447753_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="89" mass="10074" checksum="C7767A216D764E81" modified="2005-11-08" version="1">MTFDEILQKRGGENCPFWRDRESKAKLLAGLAQDAVKEAILEHGASPRNLRVEATYYDSRREPQCCYGKVTVIDDTPQTTLREAVDDGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILV6</accession>
    <name>Q3ILV6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">IS1341-type transposase ISNph18</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">NP_6268A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7 8">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324511.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILV6"/>
    <dbReference type="EnsemblBacteria" id="CAI50914">
        <property type="protein sequence ID" value="CAI50914"/>
        <property type="gene ID" value="NP_6268A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694661"/>
    <dbReference type="KEGG" id="nph:NP_6268A"/>
    <dbReference type="HOGENOM" id="CLU_032903_16_6_2"/>
    <dbReference type="OrthoDB" id="284225at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="5">
        <location>
            <begin position="183"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="6">
        <location>
            <begin position="312"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI50914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="420" mass="47558" checksum="C723C94C594BABD9" modified="2005-11-08" version="1">MSDRVVTRTLKASIRNHSQVCDGLDSHGFAASKLWNVGRWTISRVWDAIGHIPDADELCSYLKSHERYADLHSQSSQRVLQELGEAFVSWYEQDDPDANPPGYRKHGDDHPRSTVTWKNQGFKLDTQYNRVRLSKGMNMKESRYAADYILCEYTLQTDEQTLDAVESVQTVRAVWTGEEWELHFVCKMRVETPETPGEKTAGVDLGICNTAAISVGDETLLYPGNSLKEDAHYFRQQEYETEGENGPSDHAEWARQKKSRRQEHFLHALSKDIVQQCADRGVGTIAVGHPKNIREDEDWGRHGNKRLHDWAFETLLSHIEYKAQERGIDVERVDEAGLKTSKTCCECGAEADSNRVERGLYVCESCELVANSDLNAAENMRATVTPNPTQDRSNGCLAQPSVRLFEKSTGRVAPQEQVRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILY2</accession>
    <name>Q3ILY2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Isochorismatase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">entB3</name>
        <name evidence="3" type="ordered locus">NP_6214A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324485.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILY2"/>
    <dbReference type="EnsemblBacteria" id="CAI50887">
        <property type="protein sequence ID" value="CAI50887"/>
        <property type="gene ID" value="NP_6214A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694683"/>
    <dbReference type="KEGG" id="nph:NP_6214A"/>
    <dbReference type="HOGENOM" id="CLU_068979_7_1_2"/>
    <dbReference type="OrthoDB" id="9194at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="entry name" value="Isochorismatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050272">
        <property type="entry name" value="Isochorismatase-like_hydrls"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43540:SF1">
        <property type="entry name" value="ISOCHORISMATASE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43540">
        <property type="entry name" value="PEROXYUREIDOACRYLATE/UREIDOACRYLATE AMIDOHYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="Isochorismatase-like hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase-like" evidence="2">
        <location>
            <begin position="27"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="208" mass="22597" checksum="76818178AE895F1F" modified="2005-11-08" version="1">MDWPDEFEMSDDQSGFGSSVGLGERPALVVIDLINAFTDPETELGSDVGGVLEQTERLLAAFRENDLPRYFTTVAFEESYGDAGRFIEKVPALRELQLGTEAVEVDDSVAPAGDERVILKKYASAFFGTDLETELTTNRVDTLVLAGVTTSGCVRATAVDSLQHGYRTIVPADAVGDRAEGPHRANLFDIDQKYGDVVETDDVLAELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILZ2</accession>
    <name>Q3ILZ2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type transport system permease protein (Probable substrate branched-chain amino acids)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">livM2</name>
        <name evidence="7" type="synonym">abc06p2</name>
        <name evidence="7" type="ordered locus">NP_6194A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7 8">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324475.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILZ2"/>
    <dbReference type="EnsemblBacteria" id="CAI50877">
        <property type="protein sequence ID" value="CAI50877"/>
        <property type="gene ID" value="NP_6194A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694667"/>
    <dbReference type="KEGG" id="nph:NP_6194A"/>
    <dbReference type="HOGENOM" id="CLU_031365_2_0_2"/>
    <dbReference type="OrthoDB" id="30958at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06581">
        <property type="entry name" value="TM_PBP1_LivM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043428">
        <property type="entry name" value="LivM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30482:SF10">
        <property type="entry name" value="HIGH-AFFINITY BRANCHED-CHAIN AMINO ACID TRANSPORT PROTEIN BRAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30482">
        <property type="entry name" value="HIGH-AFFINITY BRANCHED-CHAIN AMINO ACID TRANSPORT SYSTEM PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="27"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="232"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="270"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="305"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI50877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="353" mass="38300" checksum="EE273A52FD86A9B6" modified="2005-11-08" version="1">MSSSDSLRAKIESVIYKADGETYDRRIVWIPIVAFLFVVPWIGVGNFELRILVGVLMWIGLAQSWNMIGGYAGYLDFGHGAYFGIGAFATGIAMSVYALPFLPALVLAGVVGAVFAYFIGVPTLRLKGAYFAIATWAFAEAMRQLALLLDITGGTSGLTLPSTMDAFGIPLPDIPRIIYIYYVMFGLCVGIMLLTYVLFERHEFGYKVKAVRDNEDAAESLGIDSTRIKRQVYVLSCATAALLGGAYAFYITFVHPQEVLDSMITAQMVIMALVGGIGTVLGPVVGGTLIFLLDRLSALFFGSDTLYISLMGLLIMIVILFAPNGIVGLLTGDTDVSDIRSNAKSLMEKFKFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMD9</accession>
    <name>Q3IMD9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Glutamate--argW ligase</fullName>
            <ecNumber evidence="12">6.3.2.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">argX</name>
        <name evidence="12" type="ordered locus">NP_5258A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="9">Amino-acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RimK family. LysX subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.-" evidence="12"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324329.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMD9"/>
    <dbReference type="STRING" id="348780.NP_5258A"/>
    <dbReference type="EnsemblBacteria" id="CAI50720">
        <property type="protein sequence ID" value="CAI50720"/>
        <property type="gene ID" value="NP_5258A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702525"/>
    <dbReference type="KEGG" id="nph:NP_5258A"/>
    <dbReference type="eggNOG" id="arCOG01589">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054353_2_1_2"/>
    <dbReference type="OrthoDB" id="33241at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043774">
        <property type="term" value="F:coenzyme F420-2 alpha-glutamyl ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009085">
        <property type="term" value="P:lysine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000025">
        <property type="entry name" value="Alpha-aminoadipate--LysW ligase LysX protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013651">
        <property type="entry name" value="ATP-grasp_RimK-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054562">
        <property type="entry name" value="LysX/ArgX_preATP_grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011870">
        <property type="entry name" value="LysX_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004666">
        <property type="entry name" value="Rp_bS6_RimK/Lys_biosynth_LsyX"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02144">
        <property type="entry name" value="LysX_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00768">
        <property type="entry name" value="rimK_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21621:SF2">
        <property type="entry name" value="COENZYME GAMMA-F420-2:ALPHA-L-GLUTAMATE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21621">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 MODIFICATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22626">
        <property type="entry name" value="LysX_preATP_grasp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08443">
        <property type="entry name" value="RimK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="11">
        <location>
            <begin position="94"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI50720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="287" mass="31675" checksum="0F6142EAE8CF66BA" modified="2005-11-08" version="1">MNVGVLYSRIRRDEKLLLSELRDRGHEVTKIDVRKERFGVHEAPAVFDGVDIVVDRCLATSRSQYITRFVDAYDVPVVNDPETAAVCADKARNSLVLAEADVPTPATEVAFTKESAMESIESFGYPCVLKPVIGSWGRLMAKIDSKDAAEAILEHKETLGHYEHKVFYIQEFVEKPGRDIRVVATDGEPIAAMARSSDHWLTNAAQGAETEAIEITDEMADLVERASEAVGGGLLGIDLMETGDSYTVHEVNHTVEFKALNEVTDVDVPSVVVDWLESRVRDVEVTA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-01-23" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMY6</accession>
    <name>RL23_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL23</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L23</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl23</name>
        <name type="ordered locus">NP_4858A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Contacts protein L29.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL23 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324132.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMY6"/>
    <dbReference type="SMR" id="Q3IMY6"/>
    <dbReference type="STRING" id="348780.NP_4858A"/>
    <dbReference type="EnsemblBacteria" id="CAI50520">
        <property type="protein sequence ID" value="CAI50520"/>
        <property type="gene ID" value="NP_4858A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703133"/>
    <dbReference type="KEGG" id="nph:NP_4858A"/>
    <dbReference type="eggNOG" id="arCOG04072">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037562_4_2_2"/>
    <dbReference type="OrthoDB" id="7751at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000532">
        <property type="entry name" value="50S ribosomal protein L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01369_A">
        <property type="entry name" value="Ribosomal_uL23_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019985">
        <property type="entry name" value="Ribosomal_uL23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013025">
        <property type="entry name" value="Ribosomal_uL23-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011118">
        <property type="entry name" value="PRK14548.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03636">
        <property type="entry name" value="uL23_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11620">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L23A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00276">
        <property type="entry name" value="Ribosomal_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000272950" description="Large ribosomal subunit protein uL23">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="82" mass="9055" checksum="07A6FF1BADB028E8" modified="2005-11-08" version="1">MTIKHPLVTEKAMNDMDFENKLQFVCHPEATKGDIAAAVESQFDVEVDSINTQVRMDGDKKAIVELSADDDAQEVASRIGVF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-18" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q3INY0</accession>
    <name>RPO11_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo11</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit L</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo11</name>
        <name evidence="1" type="synonym">rpoL</name>
        <name type="ordered locus">NP_4162A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal Rpo11/eukaryotic RPB11/RPC19 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323788.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3INY0"/>
    <dbReference type="SMR" id="Q3INY0"/>
    <dbReference type="STRING" id="348780.NP_4162A"/>
    <dbReference type="EnsemblBacteria" id="CAI50172">
        <property type="protein sequence ID" value="CAI50172"/>
        <property type="gene ID" value="NP_4162A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703160"/>
    <dbReference type="KEGG" id="nph:NP_4162A"/>
    <dbReference type="eggNOG" id="arCOG04111">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090381_5_0_2"/>
    <dbReference type="OrthoDB" id="24205at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06927">
        <property type="entry name" value="RNAP_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00261">
        <property type="entry name" value="RNApol_arch_Rpo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009025">
        <property type="entry name" value="RBP11-like_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008193">
        <property type="entry name" value="RNA_pol_Rpb11_13-16kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022905">
        <property type="entry name" value="Rpo11-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002236">
        <property type="entry name" value="PRK01146.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13656">
        <property type="entry name" value="RNA_pol_L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01154">
        <property type="entry name" value="RNA_POL_L_13KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000232469" description="DNA-directed RNA polymerase subunit Rpo11">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00261"/>
        </source>
    </evidence>
    <sequence length="94" mass="10151" checksum="9596384719066731" modified="2005-11-08" version="1">MDLRVIENLETELAIEIEGEDHTFMNVLKGALLELDGVTAATYDVNPEQSGGQTEPIVTIKTDGSIDPLDALEQGASRVRDKTDAFEEAFEAAA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IPL6</accession>
    <name>EF1B_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Elongation factor 1-beta</fullName>
            <shortName evidence="1">EF-1-beta</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">aEF-1beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ef1b</name>
        <name type="ordered locus">NP_3686A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Promotes the exchange of GDP for GTP in EF-1-alpha/GDP, thus allowing the regeneration of EF-1-alpha/GTP that could then be used to form the ternary complex EF-1-alpha/GTP/AAtRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the EF-1-beta/EF-1-delta family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323552.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IPL6"/>
    <dbReference type="SMR" id="Q3IPL6"/>
    <dbReference type="STRING" id="348780.NP_3686A"/>
    <dbReference type="EnsemblBacteria" id="CAI49934">
        <property type="protein sequence ID" value="CAI49934"/>
        <property type="gene ID" value="NP_3686A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703231"/>
    <dbReference type="KEGG" id="nph:NP_3686A"/>
    <dbReference type="eggNOG" id="arCOG01988">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165896_0_0_2"/>
    <dbReference type="OrthoDB" id="84643at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00292">
        <property type="entry name" value="EF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00043">
        <property type="entry name" value="EF1_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036219">
        <property type="entry name" value="eEF-1beta-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014038">
        <property type="entry name" value="EF1B_bsu/dsu_GNE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014717">
        <property type="entry name" value="Transl_elong_EF1B/ribsomal_bS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004542">
        <property type="entry name" value="Transl_elong_EF1B_B_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00489">
        <property type="entry name" value="aEF-1_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001670">
        <property type="entry name" value="PRK00435.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39647">
        <property type="entry name" value="ELONGATION FACTOR 1-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39647:SF1">
        <property type="entry name" value="ELONGATION FACTOR 1-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00736">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006521">
        <property type="entry name" value="Transl_elong_EF1B_B_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00888">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54984">
        <property type="entry name" value="eEF-1beta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0251">Elongation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000006619" description="Elongation factor 1-beta">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00043"/>
        </source>
    </evidence>
    <sequence length="88" mass="9402" checksum="70E4D3D88D45421C" modified="2005-11-08" version="1">MGKVAAKMKVMPQSPEVDLDELQDRLENSLPEGAKISRVDREDVAFGLIALFPTVIIPDEAGGTDTVEDAFSGVDGVESVDVDEVGRI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-06-16" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IQ04</accession>
    <name>Y3406_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0179 protein NP_3406A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">NP_3406A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0179 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323414.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IQ04"/>
    <dbReference type="STRING" id="348780.NP_3406A"/>
    <dbReference type="EnsemblBacteria" id="CAI49794">
        <property type="protein sequence ID" value="CAI49794"/>
        <property type="gene ID" value="NP_3406A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701767"/>
    <dbReference type="KEGG" id="nph:NP_3406A"/>
    <dbReference type="eggNOG" id="arCOG04477">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121764_0_0_2"/>
    <dbReference type="OrthoDB" id="24613at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00498">
        <property type="entry name" value="UPF0179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005369">
        <property type="entry name" value="UPF0179"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40699">
        <property type="entry name" value="UPF0179 PROTEIN MJ1627"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40699:SF1">
        <property type="entry name" value="UPF0179 PROTEIN MJ1627"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03684">
        <property type="entry name" value="UPF0179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006595">
        <property type="entry name" value="UCP006595"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000378133" description="UPF0179 protein NP_3406A">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00498"/>
        </source>
    </evidence>
    <sequence length="147" mass="15932" checksum="745BDEF3F5A5A0CB" modified="2005-11-08" version="1">MSTVTLLGERLAEVGTEFVYGGETDACEGCPYREQCLNLTEGRRYRVTGVRDSGTLECAVHDTGVTAVEVEPAPVLANVDANAAYAGSKASLVGPCPYTECPSHEFCEPDGADFDEEYQITEVVGDPPHDYCMLDRELQLVQFAAEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-01-24" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IQA3</accession>
    <name>RS15_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein uS15</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">30S ribosomal protein S15</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps15</name>
        <name type="ordered locus">NP_3204A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323315.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IQA3"/>
    <dbReference type="SMR" id="Q3IQA3"/>
    <dbReference type="STRING" id="348780.NP_3204A"/>
    <dbReference type="EnsemblBacteria" id="CAI49693">
        <property type="protein sequence ID" value="CAI49693"/>
        <property type="gene ID" value="NP_3204A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703171"/>
    <dbReference type="KEGG" id="nph:NP_3204A"/>
    <dbReference type="eggNOG" id="arCOG04185">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090139_2_0_2"/>
    <dbReference type="OrthoDB" id="6533at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00353">
        <property type="entry name" value="Ribosomal_S15p_S13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.10">
        <property type="entry name" value="S15/NS1, RNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01343_A">
        <property type="entry name" value="Ribosomal_uS15_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000589">
        <property type="entry name" value="Ribosomal_uS15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023029">
        <property type="entry name" value="Ribosomal_uS15_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012606">
        <property type="entry name" value="Ribosomal_uS15_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="uS15_NS1_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006331">
        <property type="entry name" value="PRK08561.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11885">
        <property type="entry name" value="RIBOSOMAL PROTEIN S15P/S13E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11885:SF6">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08069">
        <property type="entry name" value="Ribosomal_S13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00312">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01386">
        <property type="entry name" value="Ribosomal_S13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01387">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="S15/NS1 RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00362">
        <property type="entry name" value="RIBOSOMAL_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000115613" description="Small ribosomal subunit protein uS15">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="21"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="154" mass="17774" checksum="7ECFF986AB53FCF3" modified="2005-11-08" version="1">MARMHSRRRGSSGSDRPTADEPPEWSEVDEDAIEDRVVELAEEGYDPSQIGLKLRDEGVQGVPVPDVKLATGKKVTEILEENDAENELPEDLRNLMERAIRLREHMDRNPGDAQNKRALQNTESKIRRLVDYYRGDKLDEEFTYSYEDAKDRLE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IR96</accession>
    <name>NADK_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD kinase</fullName>
            <ecNumber evidence="1">2.7.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP-dependent NAD kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nadK</name>
        <name type="ordered locus">NP_2512A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NAD(+) + ATP = ADP + NADP(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18629"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.23" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322973.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IR96"/>
    <dbReference type="SMR" id="Q3IR96"/>
    <dbReference type="STRING" id="348780.NP_2512A"/>
    <dbReference type="EnsemblBacteria" id="CAI49347">
        <property type="protein sequence ID" value="CAI49347"/>
        <property type="gene ID" value="NP_2512A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703046"/>
    <dbReference type="KEGG" id="nph:NP_2512A"/>
    <dbReference type="eggNOG" id="arCOG01348">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008831_0_2_2"/>
    <dbReference type="OrthoDB" id="77798at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00361">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275:SF43">
        <property type="entry name" value="BIFUNCTIONAL NADP PHOSPHATASE_NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20143">
        <property type="entry name" value="NAD_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000229720" description="NAD kinase">
        <location>
            <begin position="1"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="61"/>
            <end position="62"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="133"/>
            <end position="134"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="176"/>
            <end position="181"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00361"/>
        </source>
    </evidence>
    <sequence length="270" mass="27955" checksum="F4E07BED9F9C3E3F" modified="2005-11-08" version="1">MELGIVAKRETPRAVELADRIRRHVDVPVTLDNLTADELDANGTDVTSLSACDLVVSIGGDGTFLFAAREVSPTPVLGVNLGEVGFLNAVSPEECVETVAGVVERMQAGDAELQELPQLQATGPGLSLPAAVNEVAVLGPQRGRDNGLDIDVRVNGEGYSSGRADGVLVSTPTGSTAYNLSEGGPIVHPDVSAFVVTEMCAESSMPSLAVPTDRTITVHVDGADHAVVAADGRTRSQVAPPAEITLAVAADPVRIAGPKLEFFTALDKLD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ISY4</accession>
    <name>Y1320_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative HTH-type transcriptional regulatory protein NP_1320A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">NP_1320A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322386.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ISY4"/>
    <dbReference type="SMR" id="Q3ISY4"/>
    <dbReference type="STRING" id="348780.NP_1320A"/>
    <dbReference type="EnsemblBacteria" id="CAI48751">
        <property type="protein sequence ID" value="CAI48751"/>
        <property type="gene ID" value="NP_1320A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701114"/>
    <dbReference type="KEGG" id="nph:NP_1320A"/>
    <dbReference type="eggNOG" id="arCOG04152">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075726_0_0_2"/>
    <dbReference type="OrthoDB" id="31424at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00584">
        <property type="entry name" value="HTH_type_cro_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020886">
        <property type="entry name" value="MTH_967-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059051">
        <property type="entry name" value="MTH_967_PDDEXK"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003162">
        <property type="entry name" value="PRK04140.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26553">
        <property type="entry name" value="PDDEXK_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000259362" description="Putative HTH-type transcriptional regulatory protein NP_1320A">
        <location>
            <begin position="1"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="132"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00584"/>
        </source>
    </evidence>
    <sequence length="317" mass="35135" checksum="7131F4435D87E7B9" modified="2005-11-08" version="1">MSRSALVGNITAMLEDAGFTVSDRCAIRPKSFDIAARRGDDVLLLKVLANIDAFDGYTGTEMRRLGEYLDATPLVIGLRTRDEELKPGVVYFRHGVPVFSPDTAMDLFIEEVPPLIYAAPGGLYVNIDGDLLSDIRSQEDMSLGKLANELGVSRRTVSKYEDGMSASVEVAAELEEIFDRKLASPVEVLSGAEEVRDDVDEPEAPEADPDDAEIVTVLTRVGFEVHPTMQAPFKAVSEDEKRERKVLTGHSEFNRTAEKRARIMSSVGHVTRTRSVYVVDSTKRDSVDGTALIEREEFERIHDSDELENLIRERAEG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IT64</accession>
    <name>NFI_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endonuclease V</fullName>
            <ecNumber evidence="1">3.1.21.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Deoxyinosine 3'endonuclease</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Deoxyribonuclease V</fullName>
            <shortName evidence="1">DNase V</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nfi</name>
        <name type="ordered locus">NP_1158A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA repair enzyme involved in the repair of deaminated bases. Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage at apurinic or apyrimidinic sites to products with a 5'-phosphate.</text>
            <dbReference type="EC" id="3.1.21.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the endonuclease V family.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.7" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322306.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IT64"/>
    <dbReference type="SMR" id="Q3IT64"/>
    <dbReference type="STRING" id="348780.NP_1158A"/>
    <dbReference type="EnsemblBacteria" id="CAI48670">
        <property type="protein sequence ID" value="CAI48670"/>
        <property type="gene ID" value="NP_1158A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702972"/>
    <dbReference type="KEGG" id="nph:NP_1158A"/>
    <dbReference type="eggNOG" id="arCOG00929">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047631_2_0_2"/>
    <dbReference type="OrthoDB" id="7885at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043737">
        <property type="term" value="F:deoxyribonuclease V activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016891">
        <property type="term" value="F:RNA endonuclease activity producing 5'-phosphomonoesters, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06559">
        <property type="entry name" value="Endonuclease_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2170.10">
        <property type="entry name" value="archaeoglobus fulgidus dsm 4304 superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00801">
        <property type="entry name" value="Endonuclease_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007581">
        <property type="entry name" value="Endonuclease-V"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28511">
        <property type="entry name" value="ENDONUCLEASE V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28511:SF1">
        <property type="entry name" value="ENDONUCLEASE V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04493">
        <property type="entry name" value="Endonuclease_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000047000" description="Endonuclease V">
        <location>
            <begin position="1"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with target DNA" evidence="1">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00801"/>
        </source>
    </evidence>
    <sequence length="248" mass="26238" checksum="CA0C4FE4397F8969" modified="2005-11-08" version="1">MELATPRFRPDPSLSREAMETLQHDIAAAASFENEASPSPAAIRDGDALVAGVDQAFLDDRAVSAVVVLRGGEVVAREHAVTPLSIPYIPGLLAFREGGPIIDALSRLDVEPDLLVVDGSGRIHFREAGLATHAGLLFDVPAVGVAKRLLCGEPSRTVASLPEGTRVPIEADDSMTAADGTVVGYAYQSRQYPDSKRINPLYISPGHRLCAETAVDCVAACGGEYKLPRPTRLADGHADDLKARYGDG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IUB0</accession>
    <name>IDI2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Isopentenyl-diphosphate delta-isomerase</fullName>
            <shortName evidence="1">IPP isomerase</shortName>
            <ecNumber evidence="1">5.3.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Isopentenyl diphosphate:dimethylallyl diphosphate isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Isopentenyl pyrophosphate isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Type 2 isopentenyl diphosphate isomerase</fullName>
            <shortName evidence="1">IDI-2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fni</name>
        <name type="ordered locus">NP_0360A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biosynthesis of isoprenoids. Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its allylic isomer, dimethylallyl diphosphate (DMAPP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>isopentenyl diphosphate = dimethylallyl diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23284"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="5.3.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homooctamer. Dimer of tetramers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the IPP isomerase type 2 family.</text>
    </comment>
    <dbReference type="EC" id="5.3.3.2" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321909.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IUB0"/>
    <dbReference type="SMR" id="Q3IUB0"/>
    <dbReference type="STRING" id="348780.NP_0360A"/>
    <dbReference type="EnsemblBacteria" id="CAI48271">
        <property type="protein sequence ID" value="CAI48271"/>
        <property type="gene ID" value="NP_0360A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702858"/>
    <dbReference type="KEGG" id="nph:NP_0360A"/>
    <dbReference type="eggNOG" id="arCOG00613">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065515_1_0_2"/>
    <dbReference type="OrthoDB" id="371955at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004452">
        <property type="term" value="F:isopentenyl-diphosphate delta-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <ligand>
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        </source>
    </evidence>
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</entry>
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        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </location>
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        </source>
    </evidence>
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            <dbReference type="EMBL" id="CAI48113.1"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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            <end position="128"/>
        </location>
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        <location>
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            <end position="199"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF04967"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF24277"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI48159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="220" mass="23413" checksum="75A5AC0070EC4B85" modified="2017-05-10" version="1">MSSPKPNADGHLRVVLAVTPSPDASCHILGSGKRGTVTKRDLRSDDDSGECTCKAEASLDDGPAQLVESDVTNGCVCPAFREHDCVASLDGFEDGTLIVSVAVPDREELTSVVAELRERGATVKLRRIDSSAVGSDRRVLRLDADGITEKQREAVQVAADAGYYETPREADLSELADQLGVSRSAVSQRLTAVETKLVEELVQAEKNGWPGPQPASVGDR</sequence>
</entry>
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    <accession>A0A1U7ETG9</accession>
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        </submittedName>
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        <name evidence="4" type="primary">recJ1</name>
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            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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                <person name="Pfeiffer F."/>
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                <person name="Rodewald K."/>
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            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAI48192.1"/>
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    </dbReference>
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    <dbReference type="STRING" id="348780.NP_0202A"/>
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        <property type="gene ID" value="NP_0202A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703098"/>
    <dbReference type="KEGG" id="nph:NP_0202A"/>
    <dbReference type="eggNOG" id="arCOG00427">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048515">
        <property type="entry name" value="DHH_CID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051673">
        <property type="entry name" value="SSDNA_exonuclease_RecJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30255">
        <property type="entry name" value="SINGLE-STRANDED-DNA-SPECIFIC EXONUCLEASE RECJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30255:SF3">
        <property type="entry name" value="SINGLE-STRANDED-DNA-SPECIFIC EXONUCLEASE RECJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21763">
        <property type="entry name" value="DHH_CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="DHH phosphoesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDH" evidence="1">
        <location>
            <begin position="28"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="DHH-CID" evidence="3">
        <location>
            <begin position="206"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="DHHA1" evidence="2">
        <location>
            <begin position="378"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="478" mass="50378" checksum="298AB63208F4F5C8" modified="2017-05-10" version="1">MAVADAPVPKLQARAADCAERLLEADSVLLASHIDADGLTSAAVAATALERAGLPFEVVFEKQLDADALAALAGREYETVLFTDFGSGQLDIIADHEAAGDFTPVIADHHQPAEATTDYHLNPLLEGIDGASELSGAGAAYLLARALASASNADERLDNRDLAALAVVGAVGDMQASGGQLQGANEDIVAEGVDAGVLTETKDLAVYGKQTRPLPKLLEYASDVRLPGITNDERGAISFLDDLDVDCRADGDWKRWVDLTDSEKQTVASGLVQYAVNRGVPAYKIERLVGTSYVLNEEEPGTELRDASEFSTLLNATARYERADVGLAVCLGDRKEALDRARELLSNHRRNLSEGIEWVEEHGVTKTDHLQWFNAGDEIRETIVGIIAGMSMGADGVSRDRPIIAFAEKGDEETKVSARGTGPLVGRGLDLSVVMGEAARAVGGDGGGHDVAAGATIPAGNEAEFVDHADEIVADQLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETK4</accession>
    <name>A0A1U7ETK4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">IS1341-type transposase ISNph9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">NP_0294A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321877.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETK4"/>
    <dbReference type="STRING" id="348780.NP_0294A"/>
    <dbReference type="EnsemblBacteria" id="CAI48238">
        <property type="protein sequence ID" value="CAI48238"/>
        <property type="gene ID" value="NP_0294A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700813"/>
    <dbReference type="KEGG" id="nph:NP_0294A"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_3_3_2"/>
    <dbReference type="OrthoDB" id="210698at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF26">
        <property type="entry name" value="TRANSPOSASE, PROBABLY IS605-TNPB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="6">
        <location>
            <begin position="178"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="7">
        <location>
            <begin position="301"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI48238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="406" mass="45979" checksum="BA956E5547E9A3F0" modified="2017-05-10" version="1">MATVELRRTAVVKLDVDDDAHRLLQETIDRFKQAAQMVADDGWNGTEDGYIVTSKTELHDRTYDDVREATDELNADLVCAARNRAADALDSCAEKRKDGKNPSKPQFTSDSVVYNLNAITYYDDYATLATVDGRIEAEYVLPDEDVPPTTYFSEDWEKREATLHHRDGDYYLHIAVVRETETEPEDAENGTVLGVDLNVDGHLAVTSTGAFIGNADYLNHKRREYEKRRGQMQQTGTRSAHLTMKSLGGRFANWSEDYLHRVSKTLVQEALAHDCTHIAFEKLTHIRERISNASKFQQWAFRRIQEYTEYKAAEYGIAVEKIAPQYTSQRCSHVDCSFTHDDNRDGDEFCCLKCDREYHADYNAAKNIARKLLQNWHKSGSGGATSHLALKSGTLNVNGIFTPTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETQ3</accession>
    <name>A0A1U7ETQ3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">GNAT family acetyltransferase</fullName>
            <ecNumber evidence="4">2.3.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_0402A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321930.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETQ3"/>
    <dbReference type="STRING" id="348780.NP_0402A"/>
    <dbReference type="EnsemblBacteria" id="CAI48292">
        <property type="protein sequence ID" value="CAI48292"/>
        <property type="gene ID" value="NP_0402A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700844"/>
    <dbReference type="KEGG" id="nph:NP_0402A"/>
    <dbReference type="eggNOG" id="arCOG00833">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_23_0_2"/>
    <dbReference type="OrthoDB" id="43754at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006464">
        <property type="entry name" value="AcTrfase_RimI/Ard1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050832">
        <property type="entry name" value="Bact_Acetyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01575">
        <property type="entry name" value="rimI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43877:SF2">
        <property type="entry name" value="AMINOALKYLPHOSPHONATE N-ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43877">
        <property type="entry name" value="AMINOALKYLPHOSPHONATE N-ACETYLTRANSFERASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="10"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="154" mass="16595" checksum="F0A50C0305EE5425" modified="2017-05-10" version="1">MRPVDSPSEVHIRQASRADLLDVLCIEEASFEQPWPYGAFERFLGTPGFLVAGDGEVVGYVVADTVDRHGATVGHIKDLAVAPSHRGRGIGRRLLSRAISTLSVAGAARVKLEVRAGNNAAKSLYRSVGFERHHTIPGYYADGEDAEVMVLSLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETV6</accession>
    <name>A0A1U7ETV6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Aspartokinase</fullName>
            <ecNumber evidence="6">2.7.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">lysC</name>
        <name evidence="9" type="ordered locus">NP_0550A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-aspartate + ATP = 4-phospho-L-aspartate + ADP</text>
            <dbReference type="Rhea" id="RHEA:23776"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57535"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the aspartokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.4" evidence="6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322002.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETV6"/>
    <dbReference type="STRING" id="348780.NP_0550A"/>
    <dbReference type="EnsemblBacteria" id="CAI48366">
        <property type="protein sequence ID" value="CAI48366"/>
        <property type="gene ID" value="NP_0550A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702893"/>
    <dbReference type="KEGG" id="nph:NP_0550A"/>
    <dbReference type="eggNOG" id="arCOG00861">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009116_6_5_2"/>
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        <property type="reaction ID" value="UER00015"/>
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        <property type="reaction ID" value="UER00461"/>
    </dbReference>
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        <property type="reaction ID" value="UER00462"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0004072">
        <property type="term" value="F:aspartate kinase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:homoserine biosynthetic process"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
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        <property type="project" value="UniProtKB-UniPathway"/>
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    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
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        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04234">
        <property type="entry name" value="AAK_AK"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
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        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054352">
        <property type="entry name" value="ACT_Aspartokinase"/>
    </dbReference>
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        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
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        <property type="entry name" value="Asp_kinase"/>
    </dbReference>
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        <property type="entry name" value="Aspartate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027795">
        <property type="entry name" value="CASTOR_ACT_dom"/>
    </dbReference>
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        <property type="entry name" value="asp_kinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005160">
        <property type="entry name" value="PRK06635.2-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21499">
        <property type="entry name" value="ASPARTATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21499:SF70">
        <property type="entry name" value="ASPARTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13840">
        <property type="entry name" value="ACT_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22468">
        <property type="entry name" value="ACT_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00324">
        <property type="entry name" value="ASPARTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACT" evidence="8">
        <location>
            <begin position="262"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="8">
        <location>
            <begin position="338"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI48366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="394" mass="41814" checksum="5BC3747BD226A8C5" modified="2017-05-10" version="1">MRVVAKFGGTSLGNGDRVNRAADSIAAAVEEGHEVAVVASAMGSTTDFLLDAIEFEADEADEAEIVSMGERTSVRMLKAALGARDINAKFLEPGTEEWPVITDEHGEVDVEATQRRAKQLAADMAAEGYVPVITGFLAQDHAGNVTTLGRGGSDTTAVMLGNYMNADEVVIITDVEGVMTGDPRVVEGARNVGEITVDELRNLSFRGAEVVAPSALSYKTADMIVRVAHYQHGDLLDGGTTIEGQFQNLIDMREEPLACLTAAGRAIRNTPGILSDLSTELSEADINVDAVASGMDSVTFYVDAEVAEEAETVLHQAVIDLDELSSVTVDDDIAVIRITGGELPNNSGILEDIINPLADAHINVLDLVTSATSVAVFLEWDDREDALEIIQDAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETW1</accession>
    <name>A0A1U7ETW1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">2-oxo-3-methylvalerate dehydrogenase E1 component beta subunit</fullName>
            <ecNumber evidence="5">1.2.4.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">oxdhB</name>
        <name evidence="5" type="ordered locus">NP_0558A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="1.2.4.4" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322006.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETW1"/>
    <dbReference type="STRING" id="348780.NP_0558A"/>
    <dbReference type="EnsemblBacteria" id="CAI48370">
        <property type="protein sequence ID" value="CAI48370"/>
        <property type="gene ID" value="NP_0558A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703005"/>
    <dbReference type="KEGG" id="nph:NP_0558A"/>
    <dbReference type="eggNOG" id="arCOG01052">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012907_1_0_2"/>
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    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003863">
        <property type="term" value="F:branched-chain 2-oxo acid dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07036">
        <property type="entry name" value="TPP_PYR_E1-PDHc-beta_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pyruvate dehydrogenase E1 beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000001">
        <property type="entry name" value="Pyruvate dehydrogenase E1 beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43257">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43257:SF2">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="4">
        <location>
            <begin position="8"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI48370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="329" mass="36227" checksum="1C6E6F687035BBB9" modified="2017-05-10" version="1">MSTETQSLTLVQAVRDGLKSEMERDEDVLVLGEDVGKNGGVFRATEGLYDEFGEDRVIDTPLAESGIIGSAIGMAAYGLRPVPEIQFSGFMYPGFDQLVSHAARLRTRSRGDFTCPMVLRAPYGGGIRAPEHHSESKEAFYTHEAGLKVVIPSTPYDTKGLLASAIRDPDPVVFLEPKLIYRAFREDVPEEPYTVPLGEAAVRRDGTDLSVFTWGAMVRPTLSAAESVAEDGIDVEVVDMRTLSPMDRETIVESFKKTGRAVVVHEAPKTGGLAGEITATIQEEALYYQEAPVNRVTGFDVPYPLYALEDYYMPEDTRIEDAIRETYEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETX9</accession>
    <name>A0A1U7ETX9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Probable iron-sulfur protein (2Fe-2S)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">NP_0600A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48391.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322027.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETX9"/>
    <dbReference type="STRING" id="348780.NP_0600A"/>
    <dbReference type="EnsemblBacteria" id="CAI48391">
        <property type="protein sequence ID" value="CAI48391"/>
        <property type="gene ID" value="NP_0600A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700902"/>
    <dbReference type="KEGG" id="nph:NP_0600A"/>
    <dbReference type="eggNOG" id="arCOG02851">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055690_4_1_2"/>
    <dbReference type="OrthoDB" id="250454at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03467">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40261">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40261:SF1">
        <property type="entry name" value="RIESKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="5">
        <location>
            <begin position="5"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI48391.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="137" mass="15081" checksum="00B7BD85C39E738A" modified="2017-05-10" version="1">MDDASHIVAVDEVPTDSTLLVTLREGFDEKEVVLVRQDDDVVAWRNYCPHWTDVRLDKGSGAEFRDEELVCTRHGAIFEADSGECSHGPCEGAYLEAVDVAVEGGEVYLTDDDYEFDHIGPKSDRDLSSGRIGFGGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETY1</accession>
    <name>A0A1U7ETY1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Bifunctional short chain isoprenyl diphosphate synthase</fullName>
            <ecNumber evidence="8">2.5.1.10</ecNumber>
            <ecNumber evidence="8">2.5.1.29</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">idsA2</name>
        <name evidence="8" type="ordered locus">NP_0604A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.10" evidence="8"/>
    <dbReference type="EC" id="2.5.1.29" evidence="8"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322029.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETY1"/>
    <dbReference type="STRING" id="348780.NP_0604A"/>
    <dbReference type="EnsemblBacteria" id="CAI48393">
        <property type="protein sequence ID" value="CAI48393"/>
        <property type="gene ID" value="NP_0604A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702861"/>
    <dbReference type="KEGG" id="nph:NP_0604A"/>
    <dbReference type="eggNOG" id="arCOG01726">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014015_0_3_2"/>
    <dbReference type="OrthoDB" id="106922at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004337">
        <property type="term" value="F:(2E,6E)-farnesyl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004311">
        <property type="term" value="F:geranylgeranyl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001:SF85">
        <property type="entry name" value="SHORT CHAIN ISOPRENYL DIPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 8" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="221"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI48393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="291" mass="31152" checksum="253A44F4D4F04DC6" modified="2017-05-10" version="1">MEYLERRRALVESRLEAVLDATEPDAMTDRLEHVSLSGGKRVRPMVALLAFEAAGGELVGEDGRGSEPSRNAIDFAVGIELVHNASLVVDDIIDESELRRGVDSAWAAFGYGPAITTSDGLLGEAFSLFSADPRAMRTVAEAMVELGEGEATELVERPDSEAAYMELARRKTGALFRAAAELGAIAADADTYAVDAFGQYAERVGIAFQIRDDVLDATADADDLGKPTGQDAEMERPSVVQITDMSPDEANERARTESDAALSSLSAADTVDSEAVGYLEDLAEFVVVRER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETZ9</accession>
    <name>A0A1U7ETZ9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Lycopene beta-cyclase</fullName>
            <ecNumber evidence="9">5.5.1.19</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">crtY</name>
        <name evidence="9" type="ordered locus">NP_0652A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Carotenoid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="5.5.1.19" evidence="9"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322053.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETZ9"/>
    <dbReference type="STRING" id="348780.NP_0652A"/>
    <dbReference type="EnsemblBacteria" id="CAI48417">
        <property type="protein sequence ID" value="CAI48417"/>
        <property type="gene ID" value="NP_0652A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702636"/>
    <dbReference type="KEGG" id="nph:NP_0652A"/>
    <dbReference type="eggNOG" id="arCOG04685">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076391_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016872">
        <property type="term" value="F:intramolecular lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045436">
        <property type="term" value="F:lycopene beta cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016117">
        <property type="term" value="P:carotenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017825">
        <property type="entry name" value="Lycopene_cyclase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03462">
        <property type="entry name" value="CarR_dom_SF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0125">Carotenoid biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="173"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI48417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="279" mass="30122" checksum="401EA48B5ECA9DC5" modified="2017-05-10" version="1">MIPPETYLGFHVLAILPPIAVLAVVTYLRPDTQWGRTPAIGLGIILALAVVYTTPWDNLLIEVGVWGYGDGVTFAHIWAAPIGEYLFFVLQPILTALWLFNLDIPTHGDLRLSARARLAGVAAGVAVSAVGIAMLAAGPSMLYLGAILAWAGPILAIQWGFGWTHLVRAWKAVVVGISVPTLYLWVADWYAISHGLWIISETYTTGIAPFGLPLEEAVFFLVTNVFVVQGLVLYLWLVDQWPTVAADVRAALPPALAELMPNDPALSGTDDRDVAAECE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU17</accession>
    <name>A0A1U7EU17_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0698A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322076.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU17"/>
    <dbReference type="STRING" id="348780.NP_0698A"/>
    <dbReference type="EnsemblBacteria" id="CAI48440">
        <property type="protein sequence ID" value="CAI48440"/>
        <property type="gene ID" value="NP_0698A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700933"/>
    <dbReference type="KEGG" id="nph:NP_0698A"/>
    <dbReference type="eggNOG" id="arCOG04738">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074280_0_0_2"/>
    <dbReference type="OrthoDB" id="154989at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043953">
        <property type="entry name" value="DUF5784"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19096">
        <property type="entry name" value="DUF5784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="331" mass="37126" checksum="EC6E91B8FF0462AE" modified="2017-05-10" version="1">MAGPLRFRRSNADWTRNRVREALLAPLEDRFGARLSEPWFAVGDDRYDAVRLEMDNGDIALFCFRPGRAYWLGNTETPEPLWRTNKETFSEAPDPVSRWAQRELTARIEVTDPWLADYEHLTWFFLPVFCSKDGRETTRRFFAEDAAGFPDADADAGLSFYESLLESGRLDDDRYTMAAKLGTSAGYDVTRMRATMSEFTVAKLFVDAGLDFDPEVEQESGHALDFAVDGRLVEVTRPEPPARRTHADHPVAAVEETGRAKRSEQLAAHPEAALFVDCTSFTDAEWSAVAAERPTVGHEPTVVFRARPSGHIEGYRHGTLPFDLDGALSTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU43</accession>
    <name>A0A1U7EU43_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycerophosphoryl diester phosphodiesterase membrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0740A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322097.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU43"/>
    <dbReference type="STRING" id="348780.NP_0740A"/>
    <dbReference type="EnsemblBacteria" id="CAI48461">
        <property type="protein sequence ID" value="CAI48461"/>
        <property type="gene ID" value="NP_0740A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700949"/>
    <dbReference type="KEGG" id="nph:NP_0740A"/>
    <dbReference type="eggNOG" id="arCOG08211">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074083_0_0_2"/>
    <dbReference type="OrthoDB" id="163788at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046157">
        <property type="entry name" value="DUF6159"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19656">
        <property type="entry name" value="DUF6159"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="71"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="144"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="194"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="283"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="303" mass="32336" checksum="4D62EAC0E8C4EF6E" modified="2017-05-10" version="1">MATNVSTNTRLGFFDRLKTGWTLTKDSLGVISDHPKLLVFPLLAGISAVVFFVLLFFPLLFAEVLGGGTEFVVLFVLYFLTTFVSTYFSAALVYASNEAFHGREPGIKKSMRAINDRLGPIAVWAIISATVSVVLRALEDSDNPLASILGALFALGWSIMTFFIVPVIVFEDVSTTSMFKESGRTFKETWGETLGAGFGISAIVLAIGLVFVAAAVALAAPVAAAFPGAGIALGVVLVLLAVVFTYLLSQTIWGVAKTALYVYAKEGVEPSQFDNFDFETLGGRTEQQATPGSGTDTPTTVRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUE8</accession>
    <name>A0A1U7EUE8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">GNAT family acetyltransferase</fullName>
            <ecNumber evidence="4">2.3.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_1004A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322228.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUE8"/>
    <dbReference type="STRING" id="348780.NP_1004A"/>
    <dbReference type="EnsemblBacteria" id="CAI48593">
        <property type="protein sequence ID" value="CAI48593"/>
        <property type="gene ID" value="NP_1004A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701029"/>
    <dbReference type="KEGG" id="nph:NP_1004A"/>
    <dbReference type="eggNOG" id="arCOG00844">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_18_3_2"/>
    <dbReference type="OrthoDB" id="11597at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050832">
        <property type="entry name" value="Bact_Acetyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43877:SF1">
        <property type="entry name" value="ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43877">
        <property type="entry name" value="AMINOALKYLPHOSPHONATE N-ACETYLTRANSFERASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="3"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="164" mass="17681" checksum="740B57FB1BD3A416" modified="2017-05-10" version="1">MSVEIRAATDDDVETVVDVAEASWYAAYGGFLSPETIARGLETSYDPDLVAAGIAHDDIAFFVAEADGDVVGFASAEQTWADEVELHTLYVHPDWWGEGAGQALLDRVREWADGVGVDRIACAVLAENEVAVGFFEAAGFVRGREASGEIGGERHPEYEFELPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUI7</accession>
    <name>A0A1U7EUI7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">CobW domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">NP_1038A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SIMIBI class G3E GTPase family. ZNG1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322245.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUI7"/>
    <dbReference type="STRING" id="348780.NP_1038A"/>
    <dbReference type="EnsemblBacteria" id="CAI48610">
        <property type="protein sequence ID" value="CAI48610"/>
        <property type="gene ID" value="NP_1038A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701034"/>
    <dbReference type="KEGG" id="nph:NP_1038A"/>
    <dbReference type="eggNOG" id="arCOG01233">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017452_2_0_2"/>
    <dbReference type="OrthoDB" id="359387at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03112">
        <property type="entry name" value="CobW-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1220.10">
        <property type="entry name" value="CobW-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036627">
        <property type="entry name" value="CobW-likC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011629">
        <property type="entry name" value="CobW-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051927">
        <property type="entry name" value="Zn_Chap_cDPG_Synth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43603">
        <property type="entry name" value="COBW DOMAIN-CONTAINING PROTEIN DDB_G0274527"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43603:SF1">
        <property type="entry name" value="ZINC-REGULATED GTPASE METALLOPROTEIN ACTIVATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07683">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00833">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90002">
        <property type="entry name" value="Hypothetical protein YjiA, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobW C-terminal" evidence="7">
        <location>
            <begin position="286"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="242"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="242"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI48610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="425" mass="46844" checksum="1C32C775147C4840" modified="2017-05-10" version="1">MSVPVTILAGTLGAGKTTLLNHVLEGDHGREVAVLVNDMGEVNVDASAIERWVDDNDVVELSNGCICCGMQGELERAVVDLALGESFEHLLVEPSGISEPSAVAEQFARGRAAGFYQLASVTTVVDAEQFYAAFGDGDARRRGAAADGTRPLSDLIVDGVEFCDRLVVNKRDRVTEAELETVVETIRTLQPDAELRRTAFGSVDPEWLLDDSAFDAEAVAGSARWKRVLEAYNDGAAQVDGHAHTAEGHDDGSHDHHSHDRAETHDHEHDHEHDHDHTHPPEQYGVESFVYRRRRPFHPERLSAVLTALPDEVVRAKGWLHVAGRPDHALHLSFAGSQTHVKVAGRWIASLDETKQDRYRRRRDPDWDDQWGDRETKLVFIGQSFDTAALEARLDDCLATEAERVDGCDGVPNPFPGAETTEVRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUJ0</accession>
    <name>A0A1U7EUJ0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cobalamin biosynthesis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1110A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322282.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUJ0"/>
    <dbReference type="STRING" id="348780.NP_1110A"/>
    <dbReference type="EnsemblBacteria" id="CAI48646">
        <property type="protein sequence ID" value="CAI48646"/>
        <property type="gene ID" value="NP_1110A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701055"/>
    <dbReference type="KEGG" id="nph:NP_1110A"/>
    <dbReference type="eggNOG" id="arCOG04749">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1168566_0_0_2"/>
    <dbReference type="OrthoDB" id="318818at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058749">
        <property type="entry name" value="Homing_endonuclease-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26411">
        <property type="entry name" value="LAGLIDADG_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="222" mass="23804" checksum="00A2F471D248006A" modified="2017-05-10" version="1">MSEHATAPKTLLDAHPETAYFWGRVAGDGEVTTGGVTVRTSDETAADRLAAIAGADAVDHETVDREYAHDTSITRNEDRYTVQLHGPVGDRAAAALGLPFEGDPGGYRLDTLTTHDRQLLRGLLEGCGTVCFKSSAGAVGVSFVHDDRALLERIQGLLDEADVDAPYGDIDETSSGGFWFGIDDGAVPAFGEWLYDGSDDDGCFAPSRRRKLRRSIEQAEAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUP1</accession>
    <name>A0A1U7EUP1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Smc operon protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1218A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322336.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUP1"/>
    <dbReference type="STRING" id="348780.NP_1218A"/>
    <dbReference type="EnsemblBacteria" id="CAI48700">
        <property type="protein sequence ID" value="CAI48700"/>
        <property type="gene ID" value="NP_1218A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701087"/>
    <dbReference type="KEGG" id="nph:NP_1218A"/>
    <dbReference type="eggNOG" id="arCOG06890">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_171539_2_0_2"/>
    <dbReference type="OrthoDB" id="188114at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055940">
        <property type="entry name" value="DUF7518"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24362">
        <property type="entry name" value="DUF7518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="53"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="100" mass="10752" checksum="14FDD301AF6BC895" modified="2017-05-10" version="1">MSGNRVEELEEKVRELEATIEGLTDELVETKERLEAVEDETGVEPDGIRRSGRSVQSQSQAAGQQPETTTDDDAADDVNNDEAGGDDNTDSESGDDIIVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUS3</accession>
    <name>A0A1U7EUS3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">AstE domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">NP_1316A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322384.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUS3"/>
    <dbReference type="STRING" id="348780.NP_1316A"/>
    <dbReference type="EnsemblBacteria" id="CAI48749">
        <property type="protein sequence ID" value="CAI48749"/>
        <property type="gene ID" value="NP_1316A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701112"/>
    <dbReference type="KEGG" id="nph:NP_1316A"/>
    <dbReference type="eggNOG" id="arCOG02890">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035605_0_2_2"/>
    <dbReference type="OrthoDB" id="314924at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055438">
        <property type="entry name" value="AstE_AspA_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043795">
        <property type="entry name" value="N-alpha-Ac-DABA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053138">
        <property type="entry name" value="N-alpha-Ac-DABA_deacetylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37326">
        <property type="entry name" value="BLL3975 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37326:SF1">
        <property type="entry name" value="BLL3975 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24827">
        <property type="entry name" value="AstE_AspA_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039012">
        <property type="entry name" value="ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Succinylglutamate desuccinylase/Aspartoacylase catalytic" evidence="6">
        <location>
            <begin position="42"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="318" mass="34246" checksum="D5907052E859E137" modified="2017-05-10" version="1">MTTLGTASAAPGEIDTGRLPVGEARDGSEVGLPVAVINGQADGKTLYMQAVSDGDELNGLGVIRRLVPQLDPTKIAGELLIVGITNYYAFQVAEHRNPIDDTKLNRAYPGSKTGTASERIAAATFDAAKQADLVLDLHQGSTSCMINETRVRCGTRHRLHRDCLELAKVFGCGHILDQKGPDGQLARAAPDEGVPTIDPELGGAVGWDETSIRKGVKGVFNVLYYYGFLDGNVDPEPQTRATGFEQYGSPAGGLVDFEFDLGDSVRRGDTLFKITDPFGTVKERVTADSEGIFWRHRRLPQVATGEYVCSLGTNLDEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUU3</accession>
    <name>A0A1U7EUU3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Probable glycosyltransferase, type 1</fullName>
            <ecNumber evidence="3">2.4.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">gth6</name>
        <name evidence="3" type="ordered locus">NP_1344A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.4.-.-" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322398.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUU3"/>
    <dbReference type="STRING" id="348780.NP_1344A"/>
    <dbReference type="EnsemblBacteria" id="CAI48763">
        <property type="protein sequence ID" value="CAI48763"/>
        <property type="gene ID" value="NP_1344A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701121"/>
    <dbReference type="KEGG" id="nph:NP_1344A"/>
    <dbReference type="eggNOG" id="arCOG01407">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_16_1_2"/>
    <dbReference type="OrthoDB" id="131038at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03801">
        <property type="entry name" value="GT4_PimA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12526:SF635">
        <property type="entry name" value="GLYCOSYL TRANSFERASE GROUP 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12526">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase subfamily 4-like N-terminal" evidence="2">
        <location>
            <begin position="25"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="1">
        <location>
            <begin position="165"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="350" mass="37573" checksum="E3FA25D8E1F3F14C" modified="2017-05-10" version="1">MHVGLVVAGDIDSGSGGFYYDRRLRERLSARGHDVTVVSVPRGSYGRQLLAALSPRRLDLRGRFDVVVEDGLAQPALLAVNRVLRVPTVAVVHMLRSQAASTAPLAVRVAERSFLRSCDAAIYNSAATKQAGTRLASPPHTAVIPPGRDRFDLPRPSRSSVRQRAGEGPLSVVFLGNVVERKGLDTLLEGLAALDADWRLTVVGDTTVDPAYTASTRKLARRLAIDDRVTYAGRLPDAEVAAHLRRGHALAVPSRYEPFGMAYLEAMGYGCVPIATTNGGPAEFIRDGETGCLVPPESPAAITDRLAELADRETLAAISTAALSAFEQHPTWEVTLDDAVDFLEAQCQTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUW6</accession>
    <name>A0A1U7EUW6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type transport system permease protein (Probable substrate biotin)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">bioN</name>
        <name evidence="7" type="synonym">abc21p</name>
        <name evidence="7" type="ordered locus">NP_1398A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322425.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUW6"/>
    <dbReference type="STRING" id="348780.NP_1398A"/>
    <dbReference type="EnsemblBacteria" id="CAI48790">
        <property type="protein sequence ID" value="CAI48790"/>
        <property type="gene ID" value="NP_1398A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702430"/>
    <dbReference type="KEGG" id="nph:NP_1398A"/>
    <dbReference type="eggNOG" id="arCOG02250">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1168562_0_0_2"/>
    <dbReference type="OrthoDB" id="204634at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16914">
        <property type="entry name" value="EcfT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003339">
        <property type="entry name" value="ABC/ECF_trnsptr_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051611">
        <property type="entry name" value="ECF_transporter_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34857">
        <property type="entry name" value="SLL0384 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34857:SF2">
        <property type="entry name" value="SLL0384 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02361">
        <property type="entry name" value="CbiQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="237" mass="25881" checksum="804EE99B7E2F8566" modified="2017-05-10" version="1">MLSYVPGDSPAHRLDPRSKLAVQLSFAALAFAYTTPTGLAVLTVFVVVLLRLAATPVVDALRAYSFVFVFLLIAPLLEGLTWGSPWFVVADAVPSALAGYRVLLVLLVAAAYVRTTPVRESRAAVQWAIPGRVGQFLGMSVSFVFRFLPVLRADIGRIRDAHRARLGTERPIPDRMRLIAVASFNRAFERADRLALALRARCFSWNPTVPQLRFGTGDLFAVIVAVSFSAAALHSML</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUX6</accession>
    <name>A0A1U7EUX6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">L-threonylcarbamoyladenylate synthase</fullName>
            <ecNumber evidence="3">2.7.7.87</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">L-threonylcarbamoyladenylate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">yrdC</name>
        <name evidence="13" type="ordered locus">NP_1420A</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonine + hydrogencarbonate + ATP = L-threonylcarbamoyladenylate + diphosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:36407"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="EC" id="2.7.7.87"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SUA5 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.87" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322436.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUX6"/>
    <dbReference type="STRING" id="348780.NP_1420A"/>
    <dbReference type="EnsemblBacteria" id="CAI48801">
        <property type="protein sequence ID" value="CAI48801"/>
        <property type="gene ID" value="NP_1420A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701144"/>
    <dbReference type="KEGG" id="nph:NP_1420A"/>
    <dbReference type="eggNOG" id="arCOG01952">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031397_3_1_2"/>
    <dbReference type="OrthoDB" id="39992at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061710">
        <property type="term" value="F:L-threonylcarbamoyladenylate synthase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.870.10">
        <property type="entry name" value="DHBP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006070">
        <property type="entry name" value="Sua5-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050156">
        <property type="entry name" value="TC-AMP_synthase_SUA5"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00057">
        <property type="entry name" value="L-threonylcarbamoyladenylate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17490">
        <property type="entry name" value="SUA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17490:SF16">
        <property type="entry name" value="THREONYLCARBAMOYL-AMP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01300">
        <property type="entry name" value="Sua5_yciO_yrdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="YrdC/RibB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51163">
        <property type="entry name" value="YRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="YrdC-like" evidence="12">
        <location>
            <begin position="3"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAI48801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="194" mass="20669" checksum="1C01E0530359829E" modified="2017-05-10" version="1">MTAPDLTDAVAALRRGDLVVYPTETVYGLAADALDPEAVKRVFEAKGRDRDKPVSMALPSVAAAAEYVTFSDRERAFCEAFLPGPVTVLLERGDRVPDVLVAGRDRVGLRVPAHDIARDLARRVGPITATSANVSGNPSARRPEAVDATIRERAVVVDGGETPGTESTVVDIAADDIVRRGAQADDIDAWLTEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUY3</accession>
    <name>A0A1U7EUY3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Probable transmembrane glycoprotein / HTH domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_1450A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322451.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUY3"/>
    <dbReference type="STRING" id="348780.NP_1450A"/>
    <dbReference type="EnsemblBacteria" id="CAI48816">
        <property type="protein sequence ID" value="CAI48816"/>
        <property type="gene ID" value="NP_1450A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701154"/>
    <dbReference type="KEGG" id="nph:NP_1450A"/>
    <dbReference type="eggNOG" id="arCOG00382">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048695_1_0_2"/>
    <dbReference type="OrthoDB" id="284722at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055767">
        <property type="entry name" value="DUF7343"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055769">
        <property type="entry name" value="DUF7345"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24034">
        <property type="entry name" value="DUF7343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24036">
        <property type="entry name" value="DUF7345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="DUF7345" evidence="3">
        <location>
            <begin position="61"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7343" evidence="2">
        <location>
            <begin position="348"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="282"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="406"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="303"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="419"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="434" mass="45757" checksum="7E39DDA40404AC07" modified="2017-05-10" version="1">MGRSRYVVLVLAVAAFAAVAAGAATAAADSPGSAAAETESADFADIDEDGFEADRTRFLITTYGNGSADWTFRYEQRLETDDDIENFGGFAARFTETETDAYTEFQARADSLAASGEEATDRRMAASRFDRDAYIEERPPAGTEFGIVEMTFRWNGFAAIEDDSVTVGDVFVGGLYVDPDNELQFEAGPGLAFESVDPEPDSMAGGTLAESETVTWVGEQEFTDRRPRLVHTEQQADRPPAEQNGDETPAPANNGSWLVPLAALLVVVLAGGTAIAYQTGTFPRRTESGGDGTAESDAGGVSTTETVDDGAAAGGTAVDDGAPDRDAGGSADTVASGEPAVTDEMLQPDEKRVVDLLERNGGRMKQVAIVDETDWSKSKVSMLLSEMEEEGTVSKLRVGRENIVSLAGHEPEAAGSPFDDGEDDTDGDGNSDGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV81</accession>
    <name>A0A1U7EV81_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transducer protein Htr34</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">htr34</name>
        <name evidence="7" type="ordered locus">NP_1642A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322546.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV81"/>
    <dbReference type="STRING" id="348780.NP_1642A"/>
    <dbReference type="EnsemblBacteria" id="CAI48912">
        <property type="protein sequence ID" value="CAI48912"/>
        <property type="gene ID" value="NP_1642A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702848"/>
    <dbReference type="KEGG" id="nph:NP_1642A"/>
    <dbReference type="eggNOG" id="arCOG02325">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_520353_0_0_2"/>
    <dbReference type="OrthoDB" id="8523at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11386">
        <property type="entry name" value="MCP_signal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.950">
        <property type="entry name" value="Methyl-accepting chemotaxis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004090">
        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32089:SF112">
        <property type="entry name" value="LYSOZYME-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32089">
        <property type="entry name" value="METHYL-ACCEPTING CHEMOTAXIS PROTEIN MCPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00260">
        <property type="entry name" value="CHEMTRNSDUCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58104">
        <property type="entry name" value="Methyl-accepting chemotaxis protein (MCP) signaling domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="Methyl-accepting transducer" evidence="6">
        <location>
            <begin position="28"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="501"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="169"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="523" mass="55134" checksum="270F4E49E6FBB76D" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVB7</accession>
    <name>A0A1U7EVB7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1736A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322592.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVB7"/>
    <dbReference type="STRING" id="348780.NP_1736A"/>
    <dbReference type="EnsemblBacteria" id="CAI48959">
        <property type="protein sequence ID" value="CAI48959"/>
        <property type="gene ID" value="NP_1736A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701243"/>
    <dbReference type="KEGG" id="nph:NP_1736A"/>
    <dbReference type="eggNOG" id="arCOG06415">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_202923_0_0_2"/>
    <dbReference type="OrthoDB" id="70008at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="67" mass="7572" checksum="26C333A6064F63D4" modified="2017-05-10" version="1">MSVAGLCEICESATVEDRCDRCARLVCADHYDRDSGLCTDCRAEYGDRPPTRRGSEDYPDGVDEYRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVC2</accession>
    <name>A0A1U7EVC2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">LWR-salt protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1764A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322606.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVC2"/>
    <dbReference type="STRING" id="348780.NP_1764A"/>
    <dbReference type="EnsemblBacteria" id="CAI48973">
        <property type="protein sequence ID" value="CAI48973"/>
        <property type="gene ID" value="NP_1764A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701249"/>
    <dbReference type="KEGG" id="nph:NP_1764A"/>
    <dbReference type="eggNOG" id="arCOG08964">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1954603_0_0_2"/>
    <dbReference type="OrthoDB" id="202660at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049798">
        <property type="entry name" value="LWR_salt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033910">
        <property type="entry name" value="LWR_salt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26423">
        <property type="entry name" value="LWR_salt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="132" mass="15061" checksum="DB7D6108AB678E29" modified="2017-05-10" version="1">MTATDADAGDAEYIFRAQVRLSPSDPDLRVEPERIETTVYRAAVSPGEDGWLFFRDTLWRGEINDPAYLRASLESALGVDIENIDFRELRTDRDYYEALKAEIGANLELFNADDTAEVQKKYLGSRIHVRDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVE2</accession>
    <name>A0A1U7EVE2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">IS1341-type transposase ISNph30</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_1814A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVE2"/>
    <dbReference type="STRING" id="348780.NP_1814A"/>
    <dbReference type="EnsemblBacteria" id="CAI48998">
        <property type="protein sequence ID" value="CAI48998"/>
        <property type="gene ID" value="NP_1814A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_1814A"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_3_3_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas12f1-like TNB" evidence="3">
        <location>
            <begin position="325"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="431"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="431"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="459" mass="52511" checksum="3256D2E56C7F2E2E" modified="2017-05-10" version="1">MLYMVESNRTVVIPLWVPEESRSDFHQTILPYAYCQQEAVNHCWPDTPKQPDDLQTSKRDVEDALYDRLREDTNEQLHSNLVQKAIKDATSAISSCKTSWENGDRISKPDFYDRDRAGYTMTYDKRAATYGRYEATLSTLNGTVTCRYELPAELEGTPYGEYVLDKRWSFSTSKLVFDGDRFWLHAVMQRQFRDDPVYTPQTDESGSDTHEDTHRVLGVDLNVDGATAVTSTGQFYGNADALNAYRSEQEQLRGELQQTGTRSAHLRFQQRRGVEWRYYDQYAHHVANSIKQEALRVRATHVAFENLTRIRKRISNLPKFQQWLFKKVRKYTEYKLERSGVTVDTTKPAHTSQACSRTDCDCVDEDNRDGKHFECLECGYTVNSDYNAAKNIGFRYLCEELSSPASRTCSSGQATSQLALVSGTLSPTGDFAEHEWVSTDKPHPQQASESSDSERSRVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVF3</accession>
    <name>A0A1U7EVF3_NATPD</name>
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        <submittedName>
            <fullName evidence="6">ArsR family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">NP_1838A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVF3"/>
    <dbReference type="STRING" id="348780.NP_1838A"/>
    <dbReference type="EnsemblBacteria" id="CAI49010">
        <property type="protein sequence ID" value="CAI49010"/>
        <property type="gene ID" value="NP_1838A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701271"/>
    <dbReference type="KEGG" id="nph:NP_1838A"/>
    <dbReference type="eggNOG" id="arCOG01685">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145184_1_0_2"/>
    <dbReference type="OrthoDB" id="11368at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051081">
        <property type="entry name" value="HTH_MetalResp_TranReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33154">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ARSR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33154:SF33">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR SDPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12840">
        <property type="entry name" value="HTH_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="5">
        <location>
            <begin position="28"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI49010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="108" mass="11423" checksum="0B8FC9756F94BDFD" modified="2017-05-10" version="1">MGVDELASPRLDRPDEPAVESVTLEDGAVIEALSSSTARQIVRSLQPAPAAASAIAEETETSIQTVSYHLDQLQKAGLVAPVGTTYSEKGTEMELYATTTESFRVVIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVF6</accession>
    <name>A0A1U7EVF6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1828A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49005.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322637.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVF6"/>
    <dbReference type="STRING" id="348780.NP_1828A"/>
    <dbReference type="EnsemblBacteria" id="CAI49005">
        <property type="protein sequence ID" value="CAI49005"/>
        <property type="gene ID" value="NP_1828A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701267"/>
    <dbReference type="KEGG" id="nph:NP_1828A"/>
    <dbReference type="eggNOG" id="arCOG06327">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_182710_0_0_2"/>
    <dbReference type="OrthoDB" id="165777at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058320">
        <property type="entry name" value="DUF8007"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26029">
        <property type="entry name" value="DUF8007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49005.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="73" mass="8212" checksum="3AE48D33656AB254" modified="2017-05-10" version="1">MGTKETEACGRCGVSSVVDTVEEEGERSATNPFEGERIEIAESEARSVLRHEVFIRKVTARLNRVVLRLTYSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVH1</accession>
    <name>A0A1U7EVH1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_1880A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322663.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVH1"/>
    <dbReference type="STRING" id="348780.NP_1880A"/>
    <dbReference type="EnsemblBacteria" id="CAI49031">
        <property type="protein sequence ID" value="CAI49031"/>
        <property type="gene ID" value="NP_1880A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701288"/>
    <dbReference type="KEGG" id="nph:NP_1880A"/>
    <dbReference type="eggNOG" id="arCOG09397">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2299418_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="100" mass="10228" checksum="7BF75C8375D37ABE" modified="2017-05-10" version="1">MEAVNTTDAIVYGFKHIATGILIVLSGVFFALVGVGLMLESPVAGIVLALIGFSIIAAGTAGLGYKVIADAVATGQQRVAAHGSSTQQQRTEKVAKKVDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVH9</accession>
    <name>A0A1U7EVH9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Small CPxCG-related zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_1878A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322662.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVH9"/>
    <dbReference type="STRING" id="348780.NP_1878A"/>
    <dbReference type="EnsemblBacteria" id="CAI49030">
        <property type="protein sequence ID" value="CAI49030"/>
        <property type="gene ID" value="NP_1878A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701287"/>
    <dbReference type="KEGG" id="nph:NP_1878A"/>
    <dbReference type="eggNOG" id="arCOG13462">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2127909_0_0_2"/>
    <dbReference type="OrthoDB" id="64860at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026870">
        <property type="entry name" value="Zinc_ribbon_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13240">
        <property type="entry name" value="Zn_Ribbon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc-ribbon" evidence="2">
        <location>
            <begin position="3"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="113" mass="11716" checksum="2E43721734534028" modified="2017-05-10" version="1">MECGNCGTEVSSEDDFCPSCGAQFSSSATGDTSSRVSGEPESRFDIGEKGYLGIGGISGVIAFGFLPPLFGVVSIFCGVQLWRHHDKKLGGGIILFGAVALIVGMYVGAQTMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVK2</accession>
    <name>A0A1U7EVK2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HTH domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_1910A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322678.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVK2"/>
    <dbReference type="EnsemblBacteria" id="CAI49046">
        <property type="protein sequence ID" value="CAI49046"/>
        <property type="gene ID" value="NP_1910A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701300"/>
    <dbReference type="KEGG" id="nph:NP_1910A"/>
    <dbReference type="eggNOG" id="arCOG04626">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078972_0_0_2"/>
    <dbReference type="OrthoDB" id="193708at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043859">
        <property type="entry name" value="TbsP-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056163">
        <property type="entry name" value="TbsP_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF047393">
        <property type="entry name" value="TransRegTbspHalo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23336">
        <property type="entry name" value="HTH_TbsP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19138">
        <property type="entry name" value="TbsP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcriptional regulator TbsP N-terminal" evidence="1">
        <location>
            <begin position="4"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Transcriptional regulator TbsP-like C-terminal" evidence="2">
        <location>
            <begin position="146"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="270" mass="29026" checksum="CD449C1F9C9CEFFB" modified="2017-05-10" version="1">METAQLESGVVGVVETAFTDGTDSLVVVDPSEETLAALVSASFDRTLPTLLVLAEERTLKDAMGDFVVASKAAALVEEGDLSLRVLSEPADNSVFAAEDQIVAPVPTGDGVAALSTDDGVVVDDVYESYHDRFETALDYDLRTPSITRVRETLEEALDEQTRADFDAVLEAVPEVRSRGDSLDEVSILLLLTAKNGNLLYDISKWGEDVGVASKATFSRTKTDLEDAGLLDTEKVPIDVGRPRLRLKLGDNRLQGRDAVDLVETAADILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVK3</accession>
    <name>A0A1U7EVK3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HiPIP domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1888A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322667.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVK3"/>
    <dbReference type="EnsemblBacteria" id="CAI49035">
        <property type="protein sequence ID" value="CAI49035"/>
        <property type="gene ID" value="NP_1888A"/>
    </dbReference>
    <dbReference type="GeneID" id="32154997"/>
    <dbReference type="KEGG" id="nph:NP_1888A"/>
    <dbReference type="eggNOG" id="arCOG10745">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123161_0_0_2"/>
    <dbReference type="OrthoDB" id="183484at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019646">
        <property type="term" value="P:aerobic electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.490.10">
        <property type="entry name" value="High potential iron-sulphur protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036369">
        <property type="entry name" value="HIPIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57652">
        <property type="entry name" value="HIPIP (high potential iron protein)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="165"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="177" mass="18885" checksum="FEFAB80EDD67292D" modified="2017-05-10" version="1">MPDRESPKAVDDSTERIADRRGVLRAAGAAAAVSVAGCLGLGDEEPAEEPDTGRDPDWCVDENDVPVPEEYRTAESIDGIERDPDELSGREEAAYQCHPQGYQLCGNCTYFIPGHRTDEQVPGACAIVEGIARSQDWCALFEPMEALEDFPSPAPFEEDGPTKPPGVGSTGTSGESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVK5</accession>
    <name>A0A1U7EVK5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Sugar nucleotidyltransferase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_1952A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVK5"/>
    <dbReference type="STRING" id="348780.NP_1952A"/>
    <dbReference type="EnsemblBacteria" id="CAI49067">
        <property type="protein sequence ID" value="CAI49067"/>
        <property type="gene ID" value="NP_1952A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_1952A"/>
    <dbReference type="eggNOG" id="arCOG00673">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_5_1_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02523">
        <property type="entry name" value="PC_cytidylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050065">
        <property type="entry name" value="GlmU-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43584:SF8">
        <property type="entry name" value="N-ACETYLMURAMATE ALPHA-1-PHOSPHATE URIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43584">
        <property type="entry name" value="NUCLEOTIDYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MobA-like NTP transferase" evidence="4">
        <location>
            <begin position="3"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="254" mass="28698" checksum="E2D35126600615A3" modified="2017-05-10" version="1">MAVHLAAGQGTRLRPLTDDRPKPLVELGGQSLLEQNVETLKEAGVRDHVVVTGYEGDQIVDRGFETVHNDVYDETEMIYSLFCAAEAFPDSGEGDLLISYGDIVYERDVVEALLDCSAPLCVAVDRQWRKLWEERFEDPLSDAETLDIDDKGRIHEIGETPEGYDTIDAQYVGLLKVRNDHIDRFVDAYTELDAQTDGYVSIDTTAFLQQLIDDGWHLQAVSIDRGWLEVDTLEDLERYRELHSSGELSQFVDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVK7</accession>
    <name>A0A1U7EVK7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 15">tRNA-splicing ligase RtcB</fullName>
            <ecNumber evidence="15">6.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">rtcB2</name>
        <name evidence="15" type="synonym">rtcB</name>
        <name evidence="16" type="ordered locus">NP_1934A</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential for tRNA splicing and maturation. Acts by directly joining spliced tRNA halves to mature-sized tRNAs. Joins RNA with 2',3'-cyclic-phosphate or 3'-phosphate ends to RNA with 5'-hydroxy ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP + H2O = a ribonucleotidyl-ribonucleotide-RNA + GMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:68080"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="Rhea" id="RHEA-COMP:17355"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:173118"/>
            <dbReference type="EC" id="6.5.1.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 3'-end 3'-phospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP = a ribonucleotidyl-ribonucleotide-RNA + GMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:68076"/>
            <dbReference type="Rhea" id="RHEA-COMP:10463"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="Rhea" id="RHEA-COMP:17355"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:83062"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:173118"/>
            <dbReference type="EC" id="6.5.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14 15">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="14 15">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 15">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 15">Belongs to the RtcB family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.-" evidence="15"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322690.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVK7"/>
    <dbReference type="STRING" id="348780.NP_1934A"/>
    <dbReference type="EnsemblBacteria" id="CAI49058">
        <property type="protein sequence ID" value="CAI49058"/>
        <property type="gene ID" value="NP_1934A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703194"/>
    <dbReference type="KEGG" id="nph:NP_1934A"/>
    <dbReference type="eggNOG" id="arCOG04246">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022279_4_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003972">
        <property type="term" value="F:RNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0170057">
        <property type="term" value="F:RNA ligase (GTP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1860.10">
        <property type="entry name" value="tRNA-splicing ligase RtcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001233">
        <property type="entry name" value="RtcB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036025">
        <property type="entry name" value="RtcB-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11118">
        <property type="entry name" value="RNA-SPLICING LIGASE RTCB HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11118:SF1">
        <property type="entry name" value="RNA-SPLICING LIGASE RTCB HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01139">
        <property type="entry name" value="RtcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103365">
        <property type="entry name" value="Hypothetical protein PH1602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 15 16" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 14 15" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="GMP-histidine intermediate" evidence="12">
        <location>
            <position position="385"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="178"/>
            <end position="182"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="367"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="385"/>
            <end position="388"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU371113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAI49058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="456" mass="50676" checksum="1FB82EDD7B7D36E0" modified="2017-05-10" version="1">MHAEQTVVVEDEFGIDDTLKDDLKTVAGIPDVERVIALGDIGPSKSETPGSIAVATTGTVYPEFCSNVVNCGMSLLRTGLKEEDVTDELLTKFCERLQSPDERFSPDKQDTIGMLEHGAEYVVDEWGFSPDLLTNIENGGNMFKTENRTREIRELVPPWILSHPNAREDTPIPNLSGNHFIEFQVVDEVLDSDTATEWGLSEGDVLVAMHGDYQLTLYINWHYANRHKFREHAALQDKIKLQLSKLLFHAWSGRVRDLPQNWKCYNGSERFSGIDTNTVQGDRYVQTQHAAMNFGYANRLLANCYMADVLADITSKASDASLLWDVGHDTLQRESIGGEEYWIHRKAAGNAAAGKPAFISGSYNMDSFLGQGLPGAEAYLNSYDHGSGNVISYFESNGQLPKTDLSTAKYSLQEARDSDKIKHIDRKPIERLADNVTGKGILSGVAWLRPIANIGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVL1</accession>
    <name>A0A1U7EVL1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Probable flippase AglR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">aglR</name>
        <name evidence="7" type="synonym">tp57</name>
        <name evidence="7" type="ordered locus">NP_1944A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322694.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVL1"/>
    <dbReference type="STRING" id="348780.NP_1944A"/>
    <dbReference type="EnsemblBacteria" id="CAI49063">
        <property type="protein sequence ID" value="CAI49063"/>
        <property type="gene ID" value="NP_1944A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703346"/>
    <dbReference type="KEGG" id="nph:NP_1944A"/>
    <dbReference type="eggNOG" id="arCOG02209">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043240_0_0_2"/>
    <dbReference type="OrthoDB" id="112053at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050833">
        <property type="entry name" value="Poly_Biosynth_Transport"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30250:SF28">
        <property type="entry name" value="POLYSACCHARIDE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30250">
        <property type="entry name" value="PST FAMILY PREDICTED COLANIC ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13440">
        <property type="entry name" value="Polysacc_synt_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="226"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="314"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="351"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="378"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="408"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="441"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="477" mass="51155" checksum="55DFAEA8CC722C9A" modified="2017-05-10" version="1">MRIGQTSVIYFLGKVTASVVGFLATIYFARVLGAEVLGFYAVAIAVAGWLKLGGSMGVSSAVQKRMSEGDDPDRYFVAGALMVAAFAAVAVSVLLLFRDQVNAYVGAEAALLVALLVTVQLLFAIVGAGLKGQRRVHVYGVLKPLKEASQSLTQVALVVPVLFGLGLTGLLAGKAIGAFLVTLLAIAVLGVSFKRPTLEHFKSLLDFAKYSWLGSVESRTFKEADIVIMGFFVSSALIGVYSIAWSLTMFLTLFGSSVRTTLFPEISNAASTKGKDAISLIEDGISYQGLILIPGFVGGTILSEQILQIYGDEFVQGTAVLWILILAVLLKGYQSQFLTALNGLDRPDISFYIYTAFILSNLILNVTLIYLFGWVGAAIATALSAGVGLSLSYIAVKRLSSFKTPTEMIAKQLTSALLMGIFVYAGLYIEQTFGLIEYNAITVGILVFSGALLYFVLLFVLSEEFRRTTRRNLPGFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVP7</accession>
    <name>A0A1U7EVP7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CcmD family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2046A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVP7"/>
    <dbReference type="EnsemblBacteria" id="CAI49114">
        <property type="protein sequence ID" value="CAI49114"/>
        <property type="gene ID" value="NP_2046A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701335"/>
    <dbReference type="KEGG" id="nph:NP_2046A"/>
    <dbReference type="eggNOG" id="arCOG11358">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_216665_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030888">
        <property type="entry name" value="Put_ccm"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04391">
        <property type="entry name" value="CcmD_alt_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="43" mass="4909" checksum="57CEE39B160B4F6B" modified="2017-05-10" version="1">MEYLLGASYGAIFAALVVYVLYLRSRLNRLESRLEDLSAERGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVR2</accession>
    <name>A0A1U7EVR2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF8071 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2082A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322760.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVR2"/>
    <dbReference type="EnsemblBacteria" id="CAI49132">
        <property type="protein sequence ID" value="CAI49132"/>
        <property type="gene ID" value="NP_2082A"/>
    </dbReference>
    <dbReference type="GeneID" id="32155000"/>
    <dbReference type="KEGG" id="nph:NP_2082A"/>
    <dbReference type="eggNOG" id="arCOG06433">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1363689_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058384">
        <property type="entry name" value="DUF8071"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26268">
        <property type="entry name" value="DUF8071"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="DUF8071" evidence="2">
        <location>
            <begin position="45"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="200" mass="20050" checksum="8B529F9092E67D92" modified="2017-05-10" version="1">MPSIPDRAESGLFRLCVLLSTAGRRVVDAARAWLCGPFKTALFGRRPMSLVAVLSGLLLAVASAAFIQTTVGTAVLAERVVETWTGESPHPPLFIAAGGLLAVGAVAAALGAGLLPTLALVGGPLFGAGVTTYGTQYAVLGQTQVVSLPQAVGDGLGVAVWFGLPIAVAAFVVGRLLRRVVDALLGGAGGAGTAGHAQRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVR7</accession>
    <name>A0A1U7EVR7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Fla cluster protein FlaG</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">flaG2</name>
        <name evidence="1" type="ordered locus">NP_2098A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322768.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVR7"/>
    <dbReference type="STRING" id="348780.NP_2098A"/>
    <dbReference type="EnsemblBacteria" id="CAI49140">
        <property type="protein sequence ID" value="CAI49140"/>
        <property type="gene ID" value="NP_2098A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702713"/>
    <dbReference type="KEGG" id="nph:NP_2098A"/>
    <dbReference type="eggNOG" id="arCOG01822">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134827_0_0_2"/>
    <dbReference type="OrthoDB" id="183655at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097588">
        <property type="term" value="P:archaeal or bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002774">
        <property type="entry name" value="Flagellin_arc-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42200:SF2">
        <property type="entry name" value="ARCHAEAL FLAGELLA-RELATED PROTEIN F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42200">
        <property type="entry name" value="ARCHAEAL FLAGELLA-RELATED PROTEIN F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01917">
        <property type="entry name" value="Flagellin_arch-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="153" mass="16133" checksum="AB79649BE0C86D7D" modified="2017-05-10" version="1">MGSVSASHLIIFIASLVVAAGVAGTLVTEVDRVSQSITEQSDGMSESIETDIQIISDTGADGIYDDGSDEVTLLVKNVGAVELPAEPERIDLLIEGQFINPDAVEAEVVGDDEVWTPGAVVRIEADTDDVDIDGETRVSVSVNENEATIQFRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVU4</accession>
    <name>A0A1U7EVU4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2122A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322780.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVU4"/>
    <dbReference type="STRING" id="348780.NP_2122A"/>
    <dbReference type="EnsemblBacteria" id="CAI49152">
        <property type="protein sequence ID" value="CAI49152"/>
        <property type="gene ID" value="NP_2122A"/>
    </dbReference>
    <dbReference type="GeneID" id="54763314"/>
    <dbReference type="KEGG" id="nph:NP_2122A"/>
    <dbReference type="eggNOG" id="arCOG08159">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_195708_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055972">
        <property type="entry name" value="DUF7550"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24418">
        <property type="entry name" value="DUF7550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="52" mass="5423" checksum="B443F49A204BA8C5" modified="2017-05-10" version="1">MSDDTDADADDAEPPERSTAPQSEYTTGQVAFGFVVALVGVAITFGMPLAFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVV7</accession>
    <name>A0A1U7EVV7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2204A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVV7"/>
    <dbReference type="STRING" id="348780.NP_2204A"/>
    <dbReference type="EnsemblBacteria" id="CAI49193">
        <property type="protein sequence ID" value="CAI49193"/>
        <property type="gene ID" value="NP_2204A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_2204A"/>
    <dbReference type="HOGENOM" id="CLU_2243937_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="104" mass="12036" checksum="C299119EFA5416E1" modified="2017-05-10" version="1">MPAADACPPEQRPVHGMRPAATPGETTRNIYDSRRRIADCHGRRDSWRSDARGHGQHRGRYCRGSIAPHHLLFQSRFAPISERFTDRTMYTRDTNRRYRTGGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVX2</accession>
    <name>A0A1U7EVX2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2226A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322831.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVX2"/>
    <dbReference type="EnsemblBacteria" id="CAI49204">
        <property type="protein sequence ID" value="CAI49204"/>
        <property type="gene ID" value="NP_2226A"/>
    </dbReference>
    <dbReference type="GeneID" id="54763317"/>
    <dbReference type="KEGG" id="nph:NP_2226A"/>
    <dbReference type="eggNOG" id="arCOG14540">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3194603_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="47" mass="5386" checksum="78EC99BE8180C0CA" modified="2017-05-10" version="1">MTRDETAPMVPIMPADDRTDDTIRADGGRRKRYDREEVLAPLVADLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVY3</accession>
    <name>A0A1U7EVY3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Acetyl-CoA C-acyltransferase</fullName>
            <ecNumber evidence="12">2.3.1.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">acaB6</name>
        <name evidence="12" type="ordered locus">NP_2260A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.16" evidence="12"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322848.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVY3"/>
    <dbReference type="STRING" id="348780.NP_2260A"/>
    <dbReference type="EnsemblBacteria" id="CAI49221">
        <property type="protein sequence ID" value="CAI49221"/>
        <property type="gene ID" value="NP_2260A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702464"/>
    <dbReference type="KEGG" id="nph:NP_2260A"/>
    <dbReference type="eggNOG" id="arCOG01282">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031026_2_2_2"/>
    <dbReference type="OrthoDB" id="25212at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003988">
        <property type="term" value="F:acetyl-CoA C-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010124">
        <property type="term" value="P:phenylacetate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000010">
        <property type="entry name" value="Acetyl-CoA acetyltransferase (Thiolase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050215">
        <property type="entry name" value="Thiolase-like_sf_Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43853">
        <property type="entry name" value="3-KETOACYL-COA THIOLASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43853:SF8">
        <property type="entry name" value="3-KETOACYL-COA THIOLASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="8" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="10">
        <location>
            <begin position="7"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="11">
        <location>
            <begin position="261"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI49221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="384" mass="41109" checksum="1A1B2DB94F176452" modified="2017-05-10" version="1">MSDNTPVVVKAYRTPFGKEDGVFADVRPEDLSVPLIDQILDETGLTGEDVDDLKWGCAQQREEQGNNMARVIALMSELGEDTPATTINRWCASSAEAVINAADAIRAGQRDCIIAGGVESMSMVKMGENNKVHPGLNDHHNIAALQMGMTAEEVAERYDVSRETQDEYAARSQQRAVEATEEGRFDDEIVPIEGHDDEGNEVLVEEDEGLRPGTTAEKLSGLPTVFKADGSVTPGNASQTTDGASALLVTSEAFAEEQGFEILAEIGNNAVAGVEPEVMGIGPIPACENLFERSGTSADDYDLVELNEAFASQTVYCQEQLGFDDEIFNVNGGAIAIGHPLGATGARLPVTLIHEMRKRDDAELGLATECVGFGQGAAIEFELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVZ2</accession>
    <name>A0A1U7EVZ2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">M50 family metalloprotease</fullName>
            <ecNumber evidence="11">3.4.24.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">NP_2278A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="11"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322857.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVZ2"/>
    <dbReference type="STRING" id="348780.NP_2278A"/>
    <dbReference type="EnsemblBacteria" id="CAI49230">
        <property type="protein sequence ID" value="CAI49230"/>
        <property type="gene ID" value="NP_2278A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701399"/>
    <dbReference type="KEGG" id="nph:NP_2278A"/>
    <dbReference type="eggNOG" id="arCOG00609">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028221_0_1_2"/>
    <dbReference type="OrthoDB" id="19110at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06160">
        <property type="entry name" value="S2P-M50_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044838">
        <property type="entry name" value="EGY1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008915">
        <property type="entry name" value="Peptidase_M50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31412">
        <property type="entry name" value="ZINC METALLOPROTEASE EGY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31412:SF0">
        <property type="entry name" value="ZINC METALLOPROTEASE EGY1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02163">
        <property type="entry name" value="Peptidase_M50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="265"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="306"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="330"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="361"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M50" evidence="10">
        <location>
            <begin position="138"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI49230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="388" mass="41698" checksum="A6C490E9FF2CCE7C" modified="2017-05-10" version="1">MDTDQSVAGDGPSPESLLPAFRTTDVERRDDGSIVYYGRPQMDSDSLERHVWPQFRDHGYEVRFDVVVDSTPDPITGVEVGQRRQALVAEPRRVGVDGVPWTNVVMFVATVLTTLYAGTIWYYQPVGGPLDLLAGWPFAAAVLGVLAVHELGHYALSRYHDVQASLPYFIPVPTFIGTFGAVISMKGRIPDREALFDIGVSGPLAGLVAAVAVAVVGLHLDPVEVPQRVLEAEEAVEIELGYPPLLEFLAWATGQQLTYEDPTVVANPVIFGAWVGLFVTFLNLIPVGQLDGGHVVRSMFGERAESVAAVVPVGLFGLAAYLFISGTSSNASVLWAFWGLIALVLSYVGHAEPVFDEPLGTPRMAVGAITFVLGVLCFTPVPIELVGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW07</accession>
    <name>A0A1U7EW07_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">UDP-glucose 6-dehydrogenase</fullName>
            <ecNumber evidence="3 7">1.1.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">aglM2</name>
        <name evidence="11" type="synonym">ugd2</name>
        <name evidence="11" type="ordered locus">NP_2322A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>UDP-alpha-D-glucose + 2 NAD(+) + H2O = UDP-alpha-D-glucuronate + 2 NADH + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:23596"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="1.1.1.22"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; UDP-alpha-D-glucuronate biosynthesis; UDP-alpha-D-glucuronate from UDP-alpha-D-glucose: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.22" evidence="3 7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322879.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW07"/>
    <dbReference type="STRING" id="348780.NP_2322A"/>
    <dbReference type="EnsemblBacteria" id="CAI49252">
        <property type="protein sequence ID" value="CAI49252"/>
        <property type="gene ID" value="NP_2322A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703443"/>
    <dbReference type="KEGG" id="nph:NP_2322A"/>
    <dbReference type="eggNOG" id="arCOG00253">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023810_1_1_2"/>
    <dbReference type="OrthoDB" id="59839at2157"/>
    <dbReference type="UniPathway" id="UPA00038">
        <property type="reaction ID" value="UER00491"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003979">
        <property type="term" value="F:UDP-glucose 6-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006065">
        <property type="term" value="P:UDP-glucuronate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.100">
        <property type="entry name" value="Cytochrome c1, transmembrane anchor, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017476">
        <property type="entry name" value="UDP-Glc/GDP-Man"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014027">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036220">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014026">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001732">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028357">
        <property type="entry name" value="UDPglc_DH_bac"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03026">
        <property type="entry name" value="NDP-sugDHase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43750">
        <property type="entry name" value="UDP-GLUCOSE 6-DEHYDROGENASE TUAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43750:SF3">
        <property type="entry name" value="UDP-GLUCOSE 6-DEHYDROGENASE TUAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00984">
        <property type="entry name" value="UDPG_MGDP_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03720">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03721">
        <property type="entry name" value="UDPG_MGDP_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500134">
        <property type="entry name" value="UDPglc_DH_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000124">
        <property type="entry name" value="UDPglc_GDPman_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00984">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52413">
        <property type="entry name" value="UDP-glucose/GDP-mannose dehydrogenase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 9" id="KW-0520">NAD</keyword>
    <keyword evidence="4 7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDP-glucose/GDP-mannose dehydrogenase C-terminal" evidence="10">
        <location>
            <begin position="321"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="258"/>
            <end position="262"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI49252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="431" mass="44528" checksum="322248AD11FE9059" modified="2017-05-10" version="1">MNVSVVGSGSLGTTVAACLADLGHEVTAVDTDEATVEALSRGETPVDEPGLGPMVTAYAGRGLTATTDHAAILETDLTMLASPTPTDEDGSPDTGALLAAAEAVGAALADADGYHLVVVKSTALPGFVESELLPTLEAASGMTEGEDFGVAVNPAFLRAGSAVDDFMEPDRIVIGTDGDDRALDRLAELYHPLVSDWDVPVVETGRQEATMIKYANSAFLAAKASLINDIGNVCKEVGVDTYAVADALGEDDRISERFLQSGVGWGGSRLPADTTALASAARDAGYEPAMLEAAIEVNDRQPQRLLSLLESHCELAGARVAVLGLASEAGSDDVRGSQAKPVIAGLRDRGASVVAYDPRAADAMAETYPDIEYADSAAAALDGADAAAFLAGWPAFADLDAEFDAMAEPVVVDGRRIVGRREGLVYEGLTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW39</accession>
    <name>A0A1U7EW39_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2424A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322929.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW39"/>
    <dbReference type="STRING" id="348780.NP_2424A"/>
    <dbReference type="EnsemblBacteria" id="CAI49303">
        <property type="protein sequence ID" value="CAI49303"/>
        <property type="gene ID" value="NP_2424A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701439"/>
    <dbReference type="KEGG" id="nph:NP_2424A"/>
    <dbReference type="HOGENOM" id="CLU_2191099_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="108" mass="10955" checksum="826489763A2910A9" modified="2017-05-10" version="1">MEREPAAYSRLVVLLGLGPLLVADLLVGLPEGAFVGGGAIVLLVAAGVHIYGGERRAGGGWVMFAAALGVAALVDLTANILYLVSFGLLLAAGLVLLASQRIIDADDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWC5</accession>
    <name>A0A1U7EWC5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UspA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2550A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322991.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWC5"/>
    <dbReference type="STRING" id="348780.NP_2550A"/>
    <dbReference type="EnsemblBacteria" id="CAI49366">
        <property type="protein sequence ID" value="CAI49366"/>
        <property type="gene ID" value="NP_2550A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701482"/>
    <dbReference type="KEGG" id="nph:NP_2550A"/>
    <dbReference type="eggNOG" id="arCOG02053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_11_0_2"/>
    <dbReference type="OrthoDB" id="105697at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF6">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN UP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="144" mass="15029" checksum="A90D48742BE57120" modified="2017-05-10" version="1">MFEEILLPVDGSEDATAAATRGFDIARAHGSRVHVICVADTGPLSDYQLPGERTSAAEALGDRAERVVESVVASAATDGIEVTTATPTGPAPETILDYADEVEADLIVMGTRGRGGVERLMLGSVTEHVVRRSDVDVLVHGGTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWD2</accession>
    <name>A0A1U7EWD2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2604A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323018.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWD2"/>
    <dbReference type="EnsemblBacteria" id="CAI49393">
        <property type="protein sequence ID" value="CAI49393"/>
        <property type="gene ID" value="NP_2604A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701505"/>
    <dbReference type="KEGG" id="nph:NP_2604A"/>
    <dbReference type="eggNOG" id="arCOG04626">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078972_0_0_2"/>
    <dbReference type="OrthoDB" id="193708at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043859">
        <property type="entry name" value="TbsP-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056163">
        <property type="entry name" value="TbsP_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF047393">
        <property type="entry name" value="TransRegTbspHalo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23336">
        <property type="entry name" value="HTH_TbsP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19138">
        <property type="entry name" value="TbsP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcriptional regulator TbsP N-terminal" evidence="1">
        <location>
            <begin position="2"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Transcriptional regulator TbsP-like C-terminal" evidence="2">
        <location>
            <begin position="144"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="268" mass="29072" checksum="C0D1F1D4AE1B302B" modified="2017-05-10" version="1">MKSNLLERTMGDVLEAALDAADDELFVINPDAEAIESLVETLDAESPPVCLFADSETLKDVLDDFLVASRAADHIEADRLEIRVNDAAANSLLVTADAVVAVVRGDDQVAGLTTDDEAFIDSAYQRITGEWADAENFNLRTPSLIRVRETLTDELGESTRENFDAVLDALETARGDGEGLDEVTISLLVAAKNEDLLYDISKWGEDVGIASKATFSRTKTRMEDNGILGTEKVPIDVGRPRLRLKLGDDNLRGADIGSLVESTQSMLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWD8</accession>
    <name>A0A1U7EWD8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2570A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323001.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWD8"/>
    <dbReference type="STRING" id="348780.NP_2570A"/>
    <dbReference type="EnsemblBacteria" id="CAI49376">
        <property type="protein sequence ID" value="CAI49376"/>
        <property type="gene ID" value="NP_2570A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701491"/>
    <dbReference type="KEGG" id="nph:NP_2570A"/>
    <dbReference type="eggNOG" id="arCOG06275">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2353158_0_0_2"/>
    <dbReference type="OrthoDB" id="206274at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055975">
        <property type="entry name" value="DUF7553"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24430">
        <property type="entry name" value="DUF7553"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="89" mass="10517" checksum="C10F33EE9A5D9B12" modified="2017-05-10" version="1">MNKHFEDTRYYLKRAGQTAKEGVAEELEPVRERVDDLIDREEEEPEPGRFEEVREDLAALQERAEGETREAIADAREKINGYREHRDDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWE8</accession>
    <name>A0A1U7EWE8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">5'-nucleotidase family hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ushA2</name>
        <name evidence="4" type="ordered locus">NP_2590A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49386.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_232503984.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWE8"/>
    <dbReference type="STRING" id="348780.NP_2590A"/>
    <dbReference type="EnsemblBacteria" id="CAI49386">
        <property type="protein sequence ID" value="CAI49386"/>
        <property type="gene ID" value="NP_2590A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703456"/>
    <dbReference type="KEGG" id="nph:NP_2590A"/>
    <dbReference type="eggNOG" id="arCOG02832">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005854_7_2_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009166">
        <property type="term" value="P:nucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00845">
        <property type="entry name" value="MPP_UshA_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.780.10">
        <property type="entry name" value="5'-Nucleotidase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008334">
        <property type="entry name" value="5'-Nucleotdase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036907">
        <property type="entry name" value="5'-Nucleotdase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006179">
        <property type="entry name" value="5_nucleotidase/apyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11575:SF24">
        <property type="entry name" value="5'-NUCLEOTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11575">
        <property type="entry name" value="5'-NUCLEOTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02872">
        <property type="entry name" value="5_nucleotid_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01607">
        <property type="entry name" value="APYRASEFAMLY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55816">
        <property type="entry name" value="5'-nucleotidase (syn. UDP-sugar hydrolase), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="5'-Nucleotidase C-terminal" evidence="3">
        <location>
            <begin position="256"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49386.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="453" mass="48292" checksum="D83BF584D699CDB6" modified="2017-05-10" version="1">MRLLHYSDIENAHDDPEHIARLAGTIQSLRDERTLVCGTGDNIAPGVVPLVADGEQALDFFAAAGPDLETFGNHDFDFGPDRLRDIVARSPQQWLSANVYEDDEPFAGVAPWTVVTVGESTVGCFGLTDPTTPSVGDPDRDIEFRDPIPAAREAVSALKGRGVDYVVALSHLGRADDRLAAAVDIDVILGGHVHSERVEHVAGTVCVRPGSGGGTLLEVDPETGAVTRHAVADGPVDVDLEATYRQRLAAAGLDSVVAHVEEPIHRTDGEAFRGESRIGNFVADAYRWAAEREFGIDYPVVGLQNSGGIRTGPPLSGPVTVADLISLVPFDEPLVVAELTGAELRAVFQEAAETPGFGESDWWHAHLSGAAVTYDYTDDRLRSATVGGTTVADEGTYRVAVSSFLTHTDVEFPTLTGDHVIAELDTQYEILVDYATDVGIAPALEGRITRVGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWG3</accession>
    <name>A0A1U7EWG3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF309 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2662A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323047.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWG3"/>
    <dbReference type="STRING" id="348780.NP_2662A"/>
    <dbReference type="EnsemblBacteria" id="CAI49422">
        <property type="protein sequence ID" value="CAI49422"/>
        <property type="gene ID" value="NP_2662A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701517"/>
    <dbReference type="KEGG" id="nph:NP_2662A"/>
    <dbReference type="eggNOG" id="arCOG04628">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1648272_0_0_2"/>
    <dbReference type="OrthoDB" id="165464at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3450.10">
        <property type="entry name" value="TTHA0068-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005500">
        <property type="entry name" value="DUF309"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023203">
        <property type="entry name" value="TTHA0068_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34796">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34796:SF1">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03745">
        <property type="entry name" value="DUF309"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140663">
        <property type="entry name" value="TTHA0068-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="164" mass="18591" checksum="EDCCB168CCA2E03A" modified="2017-05-10" version="1">MDDHTVDNDVPPPTNGDPTGWQRRRGRSNGWEHGTLRRAVVHGVRLYNDGAYHESHDCFEAEWYNYGRGTAESAFLHGMVQVAAGAYKHFDFEDDDGMRSLFETALQYLEGMPRDYYGVDVLAVRTAVTNAINDPAELAGWQIPLDGERPTARPVDFEYARRLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWH3</accession>
    <name>A0A1U7EWH3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Divalent-cation tolerance protein CutA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cutA</name>
        <name evidence="3" type="ordered locus">NP_2690A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CutA family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323061.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWH3"/>
    <dbReference type="STRING" id="348780.NP_2690A"/>
    <dbReference type="EnsemblBacteria" id="CAI49436">
        <property type="protein sequence ID" value="CAI49436"/>
        <property type="gene ID" value="NP_2690A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702644"/>
    <dbReference type="KEGG" id="nph:NP_2690A"/>
    <dbReference type="eggNOG" id="arCOG04231">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098807_3_1_2"/>
    <dbReference type="OrthoDB" id="8015at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010038">
        <property type="term" value="P:response to metal ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004323">
        <property type="entry name" value="Ion_tolerance_CutA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23419">
        <property type="entry name" value="DIVALENT CATION TOLERANCE CUTA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23419:SF8">
        <property type="entry name" value="FI09726P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03091">
        <property type="entry name" value="CutA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="GlnB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="100" mass="11084" checksum="67DE05728E060D64" modified="2017-05-10" version="1">MPTVYITAPREAADDLAADLVESEVAACVNRVDCSSTFRWEGDVVTEAEAVLLAKTTDDGYEALKARVEDQHPYDVPCIERFDEDDVLDAFGEWVAESVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWL9</accession>
    <name>A0A1U7EWL9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DUF2071 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2788A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323110.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWL9"/>
    <dbReference type="STRING" id="348780.NP_2788A"/>
    <dbReference type="EnsemblBacteria" id="CAI49485">
        <property type="protein sequence ID" value="CAI49485"/>
        <property type="gene ID" value="NP_2788A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701559"/>
    <dbReference type="KEGG" id="nph:NP_2788A"/>
    <dbReference type="eggNOG" id="arCOG04579">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081757_0_0_2"/>
    <dbReference type="OrthoDB" id="233478at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.400.10">
        <property type="entry name" value="Acetoacetate decarboxylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023375">
        <property type="entry name" value="ADC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018644">
        <property type="entry name" value="DUF2071"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39186:SF1">
        <property type="entry name" value="DUF2071 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39186">
        <property type="entry name" value="DUF2071 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09844">
        <property type="entry name" value="DUF2071"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160104">
        <property type="entry name" value="Acetoacetate decarboxylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="234" mass="26243" checksum="93F3D57DF4EA8CE6" modified="2017-05-10" version="1">MRNLLEMTWRDLLFAHWTVPPETVAETLPDGLAVDTFDGDAYLGVVPFVMSDISPRGVPFGLEFGELNLRTYVTVDGRPGVYFYNLDADDRIGVAIARALFQLPYYRAAMDIETRGEGTDREVRFRSRRTSDAPDARFDARYGPDSDFSTPDPGSLPAFLTERYRFYTTDRGGTVYYGDIDHEPWSLAPAWADIETNTLFAANGFDRPGGNPLLYFAAPIAVTADRIRRFAGPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWM1</accession>
    <name>A0A1U7EWM1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Macro domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2792A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49487.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939554.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWM1"/>
    <dbReference type="STRING" id="348780.NP_2792A"/>
    <dbReference type="EnsemblBacteria" id="CAI49487">
        <property type="protein sequence ID" value="CAI49487"/>
        <property type="gene ID" value="NP_2792A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701561"/>
    <dbReference type="KEGG" id="nph:NP_2792A"/>
    <dbReference type="eggNOG" id="arCOG04225">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046550_7_1_2"/>
    <dbReference type="OrthoDB" id="15450at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11106">
        <property type="entry name" value="GANGLIOSIDE INDUCED DIFFERENTIATION ASSOCIATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11106:SF111">
        <property type="entry name" value="MACRO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01661">
        <property type="entry name" value="Macro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00506">
        <property type="entry name" value="A1pp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51154">
        <property type="entry name" value="MACRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Macro" evidence="1">
        <location>
            <begin position="1"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49487.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="170" mass="17601" checksum="2B35B9F46B994DB6" modified="2017-05-10" version="1">MEFDVVQGDIAAQSADALVNAAGTSLKMGSGVAGALRRAAGRGLNEEAVSKGPVDLGEAAVTDAYELDAEYVIHAAAMPHYGDGEATAESIREATRNALAAADKRGCSSLVLPALGCGVAGFDLEEGAALIGETIAEYEPETLEAVEFIAYSDREYETVRAATELFRNDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWQ9</accession>
    <name>A0A1U7EWQ9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2890A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323161.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWQ9"/>
    <dbReference type="STRING" id="348780.NP_2890A"/>
    <dbReference type="EnsemblBacteria" id="CAI49536">
        <property type="protein sequence ID" value="CAI49536"/>
        <property type="gene ID" value="NP_2890A"/>
    </dbReference>
    <dbReference type="GeneID" id="77383464"/>
    <dbReference type="KEGG" id="nph:NP_2890A"/>
    <dbReference type="eggNOG" id="arCOG06262">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2056125_0_0_2"/>
    <dbReference type="OrthoDB" id="205286at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045396">
        <property type="entry name" value="DUF6517"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20127">
        <property type="entry name" value="DUF6517"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="119" mass="12497" checksum="DC18609534A0E66C" modified="2017-05-10" version="1">MKRRDVLAGVGATALLGTAGCLGVAGLDEHEATPAGVDPEVRSSTGYSQVGIEELVIREQVDLPGLSEEVVVRNYLTEHDKGIDLGPLGRLRAAVFTVLTSPQISIAGRELNSDSRDGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWV3</accession>
    <name>A0A1U7EWV3_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dolichol kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2992A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323211.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWV3"/>
    <dbReference type="STRING" id="348780.NP_2992A"/>
    <dbReference type="EnsemblBacteria" id="CAI49587">
        <property type="protein sequence ID" value="CAI49587"/>
        <property type="gene ID" value="NP_2992A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701619"/>
    <dbReference type="KEGG" id="nph:NP_2992A"/>
    <dbReference type="eggNOG" id="arCOG01881">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089196_0_0_2"/>
    <dbReference type="OrthoDB" id="213078at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004143">
        <property type="term" value="F:ATP-dependent diacylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037997">
        <property type="entry name" value="Dgk1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31303">
        <property type="entry name" value="CTP-DEPENDENT DIACYLGLYCEROL KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31303:SF1">
        <property type="entry name" value="CTP-DEPENDENT DIACYLGLYCEROL KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="196" mass="20560" checksum="FA4ABC6FE05C29D7" modified="2017-05-10" version="1">MPFEIRRRLVHASGAAVPGVYLLDNHAIGAGLITWQVVQLLAVVGLVATAVLEVARLYGGLEHAIYDQLTREYEQETVAGYALYVLGATIVVFVFEPTVAVPALFMLTLADPVSGMLSGNEFRRVARPRVFAGMFLVSFALAYPFVSAAAAVAGAVGAAIADGVKPVVGGYVVDDNLTIPVVSAALMWAARTALGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWX8</accession>
    <name>A0A1U7EWX8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DMT superfamily transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tp23</name>
        <name evidence="7" type="ordered locus">NP_3074A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323250.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWX8"/>
    <dbReference type="STRING" id="348780.NP_3074A"/>
    <dbReference type="EnsemblBacteria" id="CAI49628">
        <property type="protein sequence ID" value="CAI49628"/>
        <property type="gene ID" value="NP_3074A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703299"/>
    <dbReference type="KEGG" id="nph:NP_3074A"/>
    <dbReference type="eggNOG" id="arCOG00271">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033863_5_1_2"/>
    <dbReference type="OrthoDB" id="17861at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050638">
        <property type="entry name" value="AA-Vitamin_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32322:SF2">
        <property type="entry name" value="EAMA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32322">
        <property type="entry name" value="INNER MEMBRANE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="122"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="271"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="6">
        <location>
            <begin position="9"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="6">
        <location>
            <begin position="152"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="298" mass="30498" checksum="CA65D6E042B615E8" modified="2017-05-10" version="1">MRPLRNLGLFFLLAALWGFSFPAISAGLEALPPLLFAALRYDVGGVVLLAFLAARGADWRPRTGEDALAILIAGAFLIAGNSLLFVGQQTVPSGVAAIIYALIPVLTTALAAVLLPAEPLSARRIVGVALGLVGVAVIARPDPEGLLSAQLVGVAFVVAAALSVALGSVLLRRRTPTVGVAPMTAWAMVVGGLLLHAGSFVAGERLAHATPSAVAVVSVVYLAVFASAVGYVVYFTLLERFGPLEINLVSYVVPVVATLGGIALLGETLTAGMVAGFVLIASGFVVIKARTIADELGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX02</accession>
    <name>A0A1U7EX02_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Probable phosphoribosyltransferase (Homolog to anthranilate phosphoribosyltransferase)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">trpD3</name>
        <name evidence="8" type="ordered locus">NP_3062A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323244.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX02"/>
    <dbReference type="STRING" id="348780.NP_3062A"/>
    <dbReference type="EnsemblBacteria" id="CAI49622">
        <property type="protein sequence ID" value="CAI49622"/>
        <property type="gene ID" value="NP_3062A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703413"/>
    <dbReference type="KEGG" id="nph:NP_3062A"/>
    <dbReference type="eggNOG" id="arCOG02012">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034315_0_0_2"/>
    <dbReference type="OrthoDB" id="233227at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004048">
        <property type="term" value="F:anthranilate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1030.10:FF:000010">
        <property type="entry name" value="Anthranilate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1030.10">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.970.10">
        <property type="entry name" value="Transferase, Pyrimidine Nucleoside Phosphorylase, Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005940">
        <property type="entry name" value="Anthranilate_Pribosyl_Tfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000312">
        <property type="entry name" value="Glycosyl_Trfase_fam3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017459">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036320">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035902">
        <property type="entry name" value="Nuc_phospho_transferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43285">
        <property type="entry name" value="ANTHRANILATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43285:SF2">
        <property type="entry name" value="ANTHRANILATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02885">
        <property type="entry name" value="Glycos_trans_3N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00591">
        <property type="entry name" value="Glycos_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52418">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47648">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="2 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Glycosyl transferase family 3 N-terminal" evidence="7">
        <location>
            <begin position="29"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 3" evidence="6">
        <location>
            <begin position="108"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="354" mass="37975" checksum="310C81365A9E3CA3" modified="2017-05-10" version="1">MAATSETYGEWPLKRLMTEVVGSGHKSADDMTREQASDAFRRILDGEPDETTLGAFWLANRWKRNTPEELAAYVDVMDEGIESAAPECDPVDCGANYDGKGRSALLGVASGLVAAAAGTPVVVHSGDRVPTQKQDAYKHVLDELGVRTELAPAESASMTDTVGFGFYYQPNFAPEVAGLEDRRDQMGVRTFVNTIETLANPADASVHLGSFYHLPYAKRLINTFDAAETATVDRVVMFQGMEGYDDVRPGSTKVAVWNEGKDIDDFDIETPNYGMDFEGEDLAVDDVAADSAAITESVLAGERTDEFADAVAVNAALRIYAGGDADDIDAGLDAAREALESGAAAERLSTLQSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX50</accession>
    <name>A0A1U7EX50_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF63 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3188A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323307.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX50"/>
    <dbReference type="STRING" id="348780.NP_3188A"/>
    <dbReference type="EnsemblBacteria" id="CAI49685">
        <property type="protein sequence ID" value="CAI49685"/>
        <property type="gene ID" value="NP_3188A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701680"/>
    <dbReference type="KEGG" id="nph:NP_3188A"/>
    <dbReference type="eggNOG" id="arCOG02178">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1028982_0_0_2"/>
    <dbReference type="OrthoDB" id="308209at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002749">
        <property type="entry name" value="DUF63"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40700:SF1">
        <property type="entry name" value="DUF63 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40700">
        <property type="entry name" value="HYPOTHETICAL MEMBRANE PROTEIN, CONSERVED, DUF63 FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01889">
        <property type="entry name" value="DUF63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="247"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="271" mass="26887" checksum="BC9E2FD1636170BA" modified="2017-05-10" version="1">MVLPSDFALPALPYLAALALGGVVAVVLLYRRRPPVTERTVAALAPWMAAGGTLYALYQAGAAPEPLAPLFSAPAVYVSVGVLAGGVWAGVADRDGSTWSLTGAAGPLAASGSVLLAAALVAAALAETPDTMGTSAAGSAVILAASIAATAVAWVLLSRTAEVSATGAVGVLAVFGHTLDGISTAVGYDLLGFGEQTPLSRIILEAGAALPPDIGGGWLFALVKIALGGAIVVLFESYVREAPAEGYLVLGLVAAVGFGPGFHNLVLFALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX84</accession>
    <name>A0A1U7EX84_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Anthranilate synthase component II</fullName>
            <ecNumber evidence="2">4.1.3.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">trpG1</name>
        <name evidence="11" type="ordered locus">NP_3344A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21732"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="4.1.3.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.27" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323383.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX84"/>
    <dbReference type="STRING" id="348780.NP_3344A"/>
    <dbReference type="EnsemblBacteria" id="CAI49763">
        <property type="protein sequence ID" value="CAI49763"/>
        <property type="gene ID" value="NP_3344A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703417"/>
    <dbReference type="KEGG" id="nph:NP_3344A"/>
    <dbReference type="eggNOG" id="arCOG00086">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014340_1_2_2"/>
    <dbReference type="OrthoDB" id="3321at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004049">
        <property type="term" value="F:anthranilate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01743">
        <property type="entry name" value="GATase1_Anthranilate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000003">
        <property type="entry name" value="Anthranilate synthase component II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050472">
        <property type="entry name" value="Anth_synth/Amidotransfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053448">
        <property type="entry name" value="AS_beta_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006221">
        <property type="entry name" value="TrpG/PapA_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041322">
        <property type="entry name" value="Anth_synII_Halo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00566">
        <property type="entry name" value="trpG_papA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418:SF4">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00099">
        <property type="entry name" value="CPSGATASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="7 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="10">
        <location>
            <begin position="6"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI49763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="197" mass="21220" checksum="E6B9BC0B842DCAE6" modified="2017-05-10" version="1">MTRVVFIDNFDSFTYNLVEYVSQHDGTETAVRRNTASLSEIEALDPDAIVISPGPGHPKNDRDVGVTMDVVSELSTEVPTLGVCLGLEAAVYAHGGTISRAPEPIHGKAFPVDHDGEGVYAGLEQGFRAGRYHSLVAESVPDCFAVTATTDHEGEELVMGVRHRAHPLECVQFHPESVLTGVGHDIIANFLDTVRGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX96</accession>
    <name>A0A1U7EX96_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3408A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323415.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX96"/>
    <dbReference type="STRING" id="348780.NP_3408A"/>
    <dbReference type="EnsemblBacteria" id="CAI49795">
        <property type="protein sequence ID" value="CAI49795"/>
        <property type="gene ID" value="NP_3408A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701768"/>
    <dbReference type="KEGG" id="nph:NP_3408A"/>
    <dbReference type="eggNOG" id="arCOG02804">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1232799_0_0_2"/>
    <dbReference type="OrthoDB" id="304916at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031110">
        <property type="term" value="P:regulation of microtubule polymerization or depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048925">
        <property type="entry name" value="RdfA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036002">
        <property type="entry name" value="Stathmin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21811">
        <property type="entry name" value="RdfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101494">
        <property type="entry name" value="Stathmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="224" mass="25044" checksum="AFFDC2F23BD2F656" modified="2017-05-10" version="1">MSDRNRCKVCDVVRRYDIESPIGEYATVDEYLVARWSGAARGEAVGYRTLTGWINKRILREAYDDAALSYPGSRITDDYETLQGDDELARQELLEYLDANGVDATRVVSDFVSWSTVRHHLKDCLNAKKPDQSASTDWEQESVDTAVEQTAEKTERALRALANKGRIRDGKAATIRVRVELECPECQVRVPFDEAFQRGYVCEEHAPQPAEEDHAEPASGGGAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXA2</accession>
    <name>A0A1U7EXA2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3398A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323410.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXA2"/>
    <dbReference type="STRING" id="348780.NP_3398A"/>
    <dbReference type="EnsemblBacteria" id="CAI49790">
        <property type="protein sequence ID" value="CAI49790"/>
        <property type="gene ID" value="NP_3398A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701763"/>
    <dbReference type="KEGG" id="nph:NP_3398A"/>
    <dbReference type="HOGENOM" id="CLU_1207650_0_0_2"/>
    <dbReference type="OrthoDB" id="379562at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="229" mass="24900" checksum="92C92845A4D3C551" modified="2017-05-10" version="1">MAERELEAERERPTVVQLDDDRERDLLAGAVTEAEGTTADEDALEKFAEQLAGYRKNAERQEAWIRTLRLPRDGKPDPGEEIEIEFLLPSGDTFTRSFVVPPQVWPADNDLVRLLDAVGRTPATLTDLLGDAVPVTHDGDEWTLDIDPEPTGERANGGWSERQTTTLLIGTLVPLVVVAQVLSPIAMFVGGASLITPLLYLLGGMGLVATLGYMVTSVVQRADADLTHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXB6</accession>
    <name>A0A1U7EXB6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Probable prenyl carboxy-lyase</fullName>
            <ecNumber evidence="3">4.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3416A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.1.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323419.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXB6"/>
    <dbReference type="STRING" id="348780.NP_3416A"/>
    <dbReference type="EnsemblBacteria" id="CAI49799">
        <property type="protein sequence ID" value="CAI49799"/>
        <property type="gene ID" value="NP_3416A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703439"/>
    <dbReference type="KEGG" id="nph:NP_3416A"/>
    <dbReference type="eggNOG" id="arCOG01671">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045784_0_0_2"/>
    <dbReference type="OrthoDB" id="198775at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046281">
        <property type="term" value="P:cinnamic acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033494">
        <property type="term" value="P:ferulate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1670.10">
        <property type="entry name" value="UbiD C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002830">
        <property type="entry name" value="UbiD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049381">
        <property type="entry name" value="UbiD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049383">
        <property type="entry name" value="UbiD-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30108">
        <property type="entry name" value="3-OCTAPRENYL-4-HYDROXYBENZOATE CARBOXY-LYASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30108:SF17">
        <property type="entry name" value="FERULIC ACID DECARBOXYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20696">
        <property type="entry name" value="UbiD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20695">
        <property type="entry name" value="UbiD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143968">
        <property type="entry name" value="UbiD C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3-octaprenyl-4-hydroxybenzoate carboxy-lyase-like N-terminal" evidence="1">
        <location>
            <begin position="8"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="3-octaprenyl-4-hydroxybenzoate carboxy-lyase-like C-terminal" evidence="2">
        <location>
            <begin position="296"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="460" mass="50197" checksum="A9CFAB7FF079B5E3" modified="2017-05-10" version="1">MTAFRDHLQRLEQDGDALAVESDLHTVDEVAMVGYESLKHNGPAITYENTAGVVRLASGAYAGPDQLQPHEQKPWTRIALAVGLDTGTSYAELLEYTTGLRETTPVAENGTEPLDARPAGIDLGDLGLPVPPTCEGGMATLGIFAVGGDRTTWSPVRGRVRGPNRLRVSIPERLGEELGTRARVTVSLGVPCDALLSAYLPWFTGARQREAFPVARGPDSLSVSRHRGMLIPTSSEVVVDGRVVGDAGALEEPLAGWERLLDTTTVDIAINEIRTRNSPTVPFASLGAPLSDDIQLAGLLEAAKLHRRINSYWGVSPIEWLLIPPEARLNFCIVASEILYAGFEWQLANALFTFSDSFDKVLILDTDVPSTDLGKALQDMWVKAHPSHNWIFSTSDAPRATAPLYRQDGSTGSRLYVDATWDARWDQEYIAPRVGFEESFPEEIQAAVRESWTSMGFSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXC4</accession>
    <name>A0A1U7EXC4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type transport system ATP-binding protein (Probable substrate branched-chain amino acids)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">livF4</name>
        <name evidence="7" type="synonym">abc08a1</name>
        <name evidence="7" type="ordered locus">NP_3432A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323427.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXC4"/>
    <dbReference type="STRING" id="348780.NP_3432A"/>
    <dbReference type="EnsemblBacteria" id="CAI49807">
        <property type="protein sequence ID" value="CAI49807"/>
        <property type="gene ID" value="NP_3432A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702387"/>
    <dbReference type="KEGG" id="nph:NP_3432A"/>
    <dbReference type="eggNOG" id="arCOG00924">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OrthoDB" id="97750at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015807">
        <property type="term" value="P:L-amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03224">
        <property type="entry name" value="ABC_TM1139_LivF_branched"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052156">
        <property type="entry name" value="BCAA_Transport_ATP-bd_LivF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43820:SF2">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43820">
        <property type="entry name" value="HIGH-AFFINITY BRANCHED-CHAIN AMINO ACID TRANSPORT ATP-BINDING PROTEIN LIVF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="6"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="243" mass="26754" checksum="85B2B4F7733142AC" modified="2017-05-10" version="1">MSDALLELSDVSVTIDSAPVLSGVSLRVDPGETVALVGRNGAGKTTTFRTVMGQTDMTDGSIRMSGTEIADMKSRERSRMGIGFAPEDRRLFTSLTVEDNLRMATWGGDSIPESEFEERLDRILDIFPEMEEFLDRPAGQLSGGQQKMVTVGRALSSDPELVLVDEPFEGLAPSVREQFRNGLERIKDLDVSIVVAESNVRHAGEVADRAYVIERGEIITDTDITEEDPLEENEEVRRIFEGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXL0</accession>
    <name>A0A1U7EXL0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable S-adenosylmethionine-dependent methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3618A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323520.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXL0"/>
    <dbReference type="STRING" id="348780.NP_3618A"/>
    <dbReference type="EnsemblBacteria" id="CAI49900">
        <property type="protein sequence ID" value="CAI49900"/>
        <property type="gene ID" value="NP_3618A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701826"/>
    <dbReference type="KEGG" id="nph:NP_3618A"/>
    <dbReference type="eggNOG" id="arCOG02702">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037990_16_0_2"/>
    <dbReference type="OrthoDB" id="1018at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="1">
        <location>
            <begin position="39"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="196" mass="21367" checksum="66A1C4C8084F98D8" modified="2017-05-10" version="1">MDRFQNTRQPDWDWWGKLWPTPGATLRKCGLSSGHTLAEVGSGNGYFALPAARIVDPAPVYAVDVDPSLLEALAHLADQQNIKNIETVAGDARSLTDHLPEPVDVCLVANAFHGIEASETFVTEAVSALAADGRLVVINWQDRPREETTIAGKPRGPPADLRLAPSTCRERIESAADITLAEQFVLPPYHYALVFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXM3</accession>
    <name>A0A1U7EXM3_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Bifunctional NAD(P)H-hydrate repair enzyme</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Nicotinamide nucleotide repair protein</fullName>
        </alternativeName>
        <domain>
            <recommendedName>
                <fullName evidence="19">ADP-dependent (S)-NAD(P)H-hydrate dehydratase</fullName>
                <ecNumber evidence="19">4.2.1.136</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="19">ADP-dependent NAD(P)HX dehydratase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="19">NAD(P)H-hydrate epimerase</fullName>
                <ecNumber evidence="19">5.1.99.6</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="22" type="primary">nnrDE</name>
        <name evidence="17" type="synonym">nnrD</name>
        <name evidence="18" type="synonym">nnrE</name>
        <name evidence="22" type="ordered locus">NP_3748A</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14 19">Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="function">
        <text evidence="17">Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="function">
        <text evidence="18">Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18 19">
            <text>(6R)-NADHX = (6S)-NADHX</text>
            <dbReference type="Rhea" id="RHEA:32215"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:64075"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 18 19">
            <text>(6R)-NADPHX = (6S)-NADPHX</text>
            <dbReference type="Rhea" id="RHEA:32227"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:64077"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(6S)-NADHX + ADP = AMP + phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32223"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.136"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(6S)-NADPHX + ADP = AMP + phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32235"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.136"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18 19">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="18 19">Binds 1 potassium ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="17">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the NnrD/CARKD family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the NnrE/AIBP family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 19">In the C-terminal section; belongs to the NnrD/CARKD family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">In the N-terminal section; belongs to the NnrE/AIBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.136" evidence="19"/>
    <dbReference type="EC" id="5.1.99.6" evidence="19"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323582.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXM3"/>
    <dbReference type="STRING" id="348780.NP_3748A"/>
    <dbReference type="EnsemblBacteria" id="CAI49965">
        <property type="protein sequence ID" value="CAI49965"/>
        <property type="gene ID" value="NP_3748A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701854"/>
    <dbReference type="KEGG" id="nph:NP_3748A"/>
    <dbReference type="eggNOG" id="arCOG00018">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024853_4_1_2"/>
    <dbReference type="OrthoDB" id="15148at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052855">
        <property type="term" value="F:ADP-dependent NAD(P)H-hydrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052856">
        <property type="term" value="F:NAD(P)HX epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110051">
        <property type="term" value="P:metabolite repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046496">
        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01171">
        <property type="entry name" value="YXKO-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10260">
        <property type="entry name" value="YjeF N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01965">
        <property type="entry name" value="NADHX_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01966">
        <property type="entry name" value="NADHX_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000631">
        <property type="entry name" value="CARKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030677">
        <property type="entry name" value="Nnr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004443">
        <property type="entry name" value="YjeF_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036652">
        <property type="entry name" value="YjeF_N_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00196">
        <property type="entry name" value="yjeF_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00197">
        <property type="entry name" value="yjeF_nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592:SF0">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01256">
        <property type="entry name" value="Carb_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03853">
        <property type="entry name" value="YjeF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017184">
        <property type="entry name" value="Nnr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64153">
        <property type="entry name" value="YjeF N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51383">
        <property type="entry name" value="YJEF_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51385">
        <property type="entry name" value="YJEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 17 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 18 19" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12 17 19" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 18 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="10 17 19" id="KW-0520">NAD</keyword>
    <keyword evidence="8 17 19" id="KW-0521">NADP</keyword>
    <keyword evidence="6 17 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 18 19" id="KW-0630">Potassium</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YjeF N-terminal" evidence="21">
        <location>
            <begin position="7"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="YjeF C-terminal" evidence="20">
        <location>
            <begin position="206"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="54"/>
            <end position="58"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="124"/>
            <end position="130"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="308"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="354"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="418"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="419"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR017184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="CAI49965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="477" mass="48708" checksum="3485F2257839D780" modified="2017-05-10" version="1">MISSEEMAVVDANAEALGVPRKQLMESSGNAVARVVESVADPDASVAMVCGRGNNGGDAFVAARFLSDYDVTVSLLGRAETIRTDIARENWTALQQAAIDTTEITAPDDLSLDDADVVVDAMLGTGITGALREPEASAAEAINATDATVVAVDVPSGIDADTGERHGVAVAADHVVTFHDEKPGLEAIAVPVTVADIGIPDAAERFVERGDLLRLSRDPTAHKGDFGRVLVVGGGPYAGAPALSAQAALRAGADLAFVACPEAVAGEVQGYSENLIVEPLPGDRLAPPHVPDLLERAAAVDCVVFGPGLGDDEATLSAVEAFFEGFEGTAVVDADALQVVPDVETDAELLCTPHQGELRKMGSETAADPAERASLVSAFAADLGVTLLLKGAEDIITDGDRTRRNRTGNPGMTVGGTGDVLAGAAGALACVLDTQDAAAVAAYANGRAGDIVVEEQGYGLVATDLTAALPKALWPDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXN1</accession>
    <name>A0A1U7EXN1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">IS1341-type transposase ISNph15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">NP_3712A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323565.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXN1"/>
    <dbReference type="STRING" id="348780.NP_3712A"/>
    <dbReference type="EnsemblBacteria" id="CAI49947">
        <property type="protein sequence ID" value="CAI49947"/>
        <property type="gene ID" value="NP_3712A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701846"/>
    <dbReference type="KEGG" id="nph:NP_3712A"/>
    <dbReference type="eggNOG" id="arCOG00684">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_0_1_2"/>
    <dbReference type="OrthoDB" id="33505at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF11">
        <property type="entry name" value="RNA-GUIDED DNA ENDONUCLEASE RV2885C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="6">
        <location>
            <begin position="185"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="7">
        <location>
            <begin position="302"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="412" mass="47178" checksum="EF3132F1669829A3" modified="2017-05-10" version="1">MKHTLRFRAYLPDDVASEAWRHIDILRQIRNHAVRDYYNADYNDRPSDYDQHSKLKDWTDQWPTFADPSQHAAQQAISQIHSDIETLQERRNEGYDVGRLQWQGAGEFRSVSYNQASRFNVDYNTGDDRFVRLRLEKIGWFKIRADRDVPPEEDIDEVILKKETTGEWYVSLVTTVEDTPEKPPLSEIEAEDCVGVDLGITSYIHTSENLSVDTLDLSDEYDRYAREQRKLDRKEHGSANWEKQRQKVAQAKRKIKRKVRDYQHKLTTWLVTEHDVVAVEDLDVKPMLEASQNAKNKQDAAWSQFIELLEYKADLHGTHVETVKPGGTTKECAVCGVETAKPIWVREHSCPACGHTEARDLNAAKNILYRGLKQLGAGRSESTPVQTALPTLTPHREAVSAKRVVEAGSPGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXN8</accession>
    <name>A0A1U7EXN8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">SBDS family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_3740A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SDO1/SBDS family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323578.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXN8"/>
    <dbReference type="STRING" id="348780.NP_3740A"/>
    <dbReference type="EnsemblBacteria" id="CAI49961">
        <property type="protein sequence ID" value="CAI49961"/>
        <property type="gene ID" value="NP_3740A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701852"/>
    <dbReference type="KEGG" id="nph:NP_3740A"/>
    <dbReference type="eggNOG" id="arCOG04187">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043216_2_0_2"/>
    <dbReference type="OrthoDB" id="84504at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1250.10">
        <property type="entry name" value="Ribosome maturation protein SBDS, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.900">
        <property type="entry name" value="SBDS protein C-terminal domain, subdomain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036786">
        <property type="entry name" value="Ribosome_mat_SBDS_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002140">
        <property type="entry name" value="Sdo1/SBDS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039100">
        <property type="entry name" value="Sdo1/SBDS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046928">
        <property type="entry name" value="SDO1/SBDS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018978">
        <property type="entry name" value="SDO1/SBDS_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037188">
        <property type="entry name" value="Sdo1/SBDS_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019783">
        <property type="entry name" value="SDO1/SBDS_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00291">
        <property type="entry name" value="RNA_SBDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10927">
        <property type="entry name" value="RIBOSOME MATURATION PROTEIN SBDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10927:SF4">
        <property type="entry name" value="RIBOSOME MATURATION PROTEIN SDO1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20268">
        <property type="entry name" value="SBDS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09377">
        <property type="entry name" value="SBDS_domain_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01172">
        <property type="entry name" value="SBDS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89895">
        <property type="entry name" value="FYSH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109728">
        <property type="entry name" value="Hypothetical protein AF0491, middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosome maturation protein SDO1/SBDS N-terminal" evidence="2">
        <location>
            <begin position="7"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosome maturation protein SDO1/SBDS central" evidence="3">
        <location>
            <begin position="102"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosome maturation protein SDO1/SBDS C-terminal" evidence="4">
        <location>
            <begin position="168"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="241" mass="26963" checksum="C4758F56A3409F81" modified="2017-05-10" version="1">MISLDEAVTARLESHGERFEVLVDPDAALSIKRGEFDGDLEDVIAAEDVFEDASSGDRPPESALEEVFETTDPLEIIPAVIKRGEIQITAEQRRKMQEQKHNQLVNKIARNAVNPQMDDAPHPPERIERALEEAGFQVDPMEPVENQVDEALDDLRPVIPIRFDEVVMAVQVPADYAGSAQAQIRQFGELQSEEWQSDGSWVGVLKFPAGLQNEFYDLVNEHTSGEAETRTVKDEDEIGTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXQ2</accession>
    <name>A0A1U7EXQ2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Sir2-type NAD-dependent protein deacetylase</fullName>
            <ecNumber evidence="5">3.5.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">sir2</name>
        <name evidence="5" type="ordered locus">NP_3762A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.1.-" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323589.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXQ2"/>
    <dbReference type="STRING" id="348780.NP_3762A"/>
    <dbReference type="EnsemblBacteria" id="CAI49972">
        <property type="protein sequence ID" value="CAI49972"/>
        <property type="gene ID" value="NP_3762A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701859"/>
    <dbReference type="KEGG" id="nph:NP_3762A"/>
    <dbReference type="eggNOG" id="arCOG04248">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023643_3_0_2"/>
    <dbReference type="OrthoDB" id="728at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017136">
        <property type="term" value="F:histone deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01407">
        <property type="entry name" value="SIR2-fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001753">
        <property type="entry name" value="PRK00481.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF10">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="251" mass="26935" checksum="D302FB4BE2023F93" modified="2017-05-10" version="1">MDQTLSAAANAVRDAETVAALTGAGVSTASGIPDFRSEDGLWNEYDPNDFHMSRFRADPAGFWRDRVELIADLFGDGVEPNAAHEALADLEAAGHLNTLITQNVDGLHQAAGSDDPIEIHGNGRRAACTGCNRRIDIDEAVQRVTAGEAPPTCERCGDVLKPDVVLFGEQLPKHDLMRAQSAAREADVFLAVGSSLTVEPAASLPRHTVDNGGQLVVVNLDRTEQSKRADFDLRADVTEALPRLTETVLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXS2</accession>
    <name>A0A1U7EXS2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">tRNA/rRNA pseudouridine synthase complex protein Gar1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gar1</name>
        <name evidence="1" type="ordered locus">NP_3810A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXS2"/>
    <dbReference type="STRING" id="348780.NP_3810A"/>
    <dbReference type="EnsemblBacteria" id="CAI49996">
        <property type="protein sequence ID" value="CAI49996"/>
        <property type="gene ID" value="NP_3810A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701872"/>
    <dbReference type="KEGG" id="nph:NP_3810A"/>
    <dbReference type="eggNOG" id="arCOG02466">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165884_3_1_2"/>
    <dbReference type="OrthoDB" id="60264at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.230">
        <property type="entry name" value="Probable tRNA pseudouridine synthase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Gar1/Naf1_Cbf5-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007504">
        <property type="entry name" value="H/ACA_rnp_Gar1/Naf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009628">
        <property type="entry name" value="PRK13149.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04410">
        <property type="entry name" value="Gar1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="77" mass="8172" checksum="520C15FD46411856" modified="2017-05-10" version="1">MKRAGEVVRIAQGLLVARLADGDDDPPDIGTSVVNEELDSVGRIVDVFGPVDRPYVAVSPADDVVPPSLLGEKLYAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXY7</accession>
    <name>A0A1U7EXY7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC-type transport system permease protein (Probable substrate copper)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">nosY2</name>
        <name evidence="3" type="synonym">abc12p</name>
        <name evidence="3" type="ordered locus">NP_3926A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323671.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXY7"/>
    <dbReference type="STRING" id="348780.NP_3926A"/>
    <dbReference type="EnsemblBacteria" id="CAI50054">
        <property type="protein sequence ID" value="CAI50054"/>
        <property type="gene ID" value="NP_3926A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702406"/>
    <dbReference type="KEGG" id="nph:NP_3926A"/>
    <dbReference type="eggNOG" id="arCOG02436">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_923218_0_0_2"/>
    <dbReference type="OrthoDB" id="313530at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12679">
        <property type="entry name" value="ABC2_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="70"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="168"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="201"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="268"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="291" mass="30222" checksum="5176CFCBAADC910D" modified="2017-05-10" version="1">MSTPTDTANTAVSEAETRPETPTTAAILRALVSRELSTAVLRRSTLLLWVGFLGVLLGIAWFGGGMQVGYIATVIDLLTPLELLVPVIAMALGYRAILSDERRGELDVLKTYPVASWQVVAGVYVGRAVGLVALVGSSLLFLLYPIFLTDAHQSLFYATHTGADSVWLYLRFVVLTVGFALVLLAVAIAISSLVGSTRSAVTAAGLGLFVLLFGADLALVYGLSRGFVDPSSLASSLAISPLSAYRSLVIETTVTVTAGTGPQTASPVAAAIGLLAWGVGSLAVAAAAIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY44</accession>
    <name>A0A1U7EY44_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4062A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323738.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY44"/>
    <dbReference type="STRING" id="348780.NP_4062A"/>
    <dbReference type="EnsemblBacteria" id="CAI50122">
        <property type="protein sequence ID" value="CAI50122"/>
        <property type="gene ID" value="NP_4062A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701955"/>
    <dbReference type="KEGG" id="nph:NP_4062A"/>
    <dbReference type="HOGENOM" id="CLU_2140276_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="112" mass="11102" checksum="700E68A5894B3F77" modified="2017-05-10" version="1">MGSDETTYGRRARIIVVVLVVITGLSALARAVEGGATGAIEGGLLLYVALVLAYGVFTGALETPSVQAAFGVGLAGYGGLLYGTGGGVLWLGLAAVGGLLAAHSLRQAVEAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY54</accession>
    <name>A0A1U7EY54_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type transport system permease protein (Probable substrate branched-chain amino acids)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">livM1</name>
        <name evidence="7" type="synonym">abc05p2</name>
        <name evidence="7" type="ordered locus">NP_4134A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323774.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY54"/>
    <dbReference type="STRING" id="348780.NP_4134A"/>
    <dbReference type="EnsemblBacteria" id="CAI50158">
        <property type="protein sequence ID" value="CAI50158"/>
        <property type="gene ID" value="NP_4134A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702380"/>
    <dbReference type="KEGG" id="nph:NP_4134A"/>
    <dbReference type="eggNOG" id="arCOG01273">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031365_1_0_2"/>
    <dbReference type="OrthoDB" id="239932at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06581">
        <property type="entry name" value="TM_PBP1_LivM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043428">
        <property type="entry name" value="LivM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30482:SF10">
        <property type="entry name" value="HIGH-AFFINITY BRANCHED-CHAIN AMINO ACID TRANSPORT PROTEIN BRAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30482">
        <property type="entry name" value="HIGH-AFFINITY BRANCHED-CHAIN AMINO ACID TRANSPORT SYSTEM PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="223"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="274"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="312"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="387"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI50158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="435" mass="45920" checksum="35A2115A40A06F9C" modified="2017-05-10" version="1">MSDSLLGRIGGALEERDLLLVLLTLGLVYVLFALSVLDQGLGSIVSTLARVTFLSLSFALVALALNLHWGYTGLFNIGVVAFMMVGVYTMAMLTRPPDPAFGAPGLGLPLWVGILAGVAVAAILGLLTALPALRLRADYLAIVTLGLSEIVRLTIASGDFNEWLIENVGAGTGSATSLTFTVSPQTPIRGLYFTDAGDPTALGTAVFGVTDDLGISQSAVVDWTYILALAVVLIAVYLFLERLGRSPFGRILKAIREDEVVASSLGKDIRMAKIKVFVIGCALMGLGGILWYGAEGAANPQAMTYSPLYTFYIFIAVIVGGAGSNTGSVLGGIVFASFLFEGPRRAGGFVRDRIAADVDTPENFAEAVAPFSSLDFTPFIAYATDEIAALQFVLLGLVLIFIMHRRPDGLLGHRVETASAVDLSERPETGGDDNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY57</accession>
    <name>A0A1U7EY57_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Purine-binding taxis protein CheW</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cheW</name>
        <name evidence="2" type="ordered locus">NP_4146A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323780.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY57"/>
    <dbReference type="STRING" id="348780.NP_4146A"/>
    <dbReference type="EnsemblBacteria" id="CAI50164">
        <property type="protein sequence ID" value="CAI50164"/>
        <property type="gene ID" value="NP_4146A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702603"/>
    <dbReference type="KEGG" id="nph:NP_4146A"/>
    <dbReference type="eggNOG" id="arCOG02395">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048995_3_2_2"/>
    <dbReference type="OrthoDB" id="115049at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.180">
        <property type="entry name" value="CheA-289, Domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039315">
        <property type="entry name" value="CheW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036061">
        <property type="entry name" value="CheW-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002545">
        <property type="entry name" value="CheW-lke_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22617:SF23">
        <property type="entry name" value="CHEMOTAXIS PROTEIN CHEW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22617">
        <property type="entry name" value="CHEMOTAXIS SENSOR HISTIDINE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01584">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00260">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50341">
        <property type="entry name" value="CheW-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50851">
        <property type="entry name" value="CHEW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CheW-like" evidence="1">
        <location>
            <begin position="11"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="160" mass="17862" checksum="666BC2DCF72675F9" modified="2017-05-10" version="1">MSEDQPQTTETKQVLEFRLGDETYCVSIDFVTEIVDMGELTPIPNSPPHVEGVIDLRGNTTSIIDPKVGLDIEGELGERIVIFDSEIFDDERSVGWAVDSVEEVSDIDLEEVDDSPVEQEHIYGLIKREEDFVVWIDPKTMDNEFAAGEELATPDAVSES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY66</accession>
    <name>A0A1U7EY66_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Thioredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">trxA4</name>
        <name evidence="9" type="ordered locus">NP_4120A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323767.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY66"/>
    <dbReference type="STRING" id="348780.NP_4120A"/>
    <dbReference type="EnsemblBacteria" id="CAI50151">
        <property type="protein sequence ID" value="CAI50151"/>
        <property type="gene ID" value="NP_4120A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703425"/>
    <dbReference type="KEGG" id="nph:NP_4120A"/>
    <dbReference type="eggNOG" id="arCOG01972">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090389_10_4_2"/>
    <dbReference type="OrthoDB" id="35385at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005746">
        <property type="entry name" value="Thioredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01068">
        <property type="entry name" value="thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45663">
        <property type="entry name" value="GEO12009P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45663:SF11">
        <property type="entry name" value="GEO12009P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000077">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4 6" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Thioredoxin" evidence="8">
        <location>
            <begin position="8"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="41"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="44"/>
        </location>
    </feature>
    <feature type="site" description="Contributes to redox potential value" evidence="5">
        <location>
            <position position="35"/>
        </location>
    </feature>
    <feature type="site" description="Contributes to redox potential value" evidence="5">
        <location>
            <position position="42"/>
        </location>
    </feature>
    <feature type="site" description="Contributes to redox potential value" evidence="5">
        <location>
            <position position="43"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="6">
        <location>
            <begin position="41"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000077-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000077-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI50151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="112" mass="11941" checksum="843FC7ABD5799F2A" modified="2017-05-10" version="1">MSSESASTSPSEPIPIEETNGLDAVTSEYDTVLVDFYADWCGPCQMMEPAVESVAESTDAAVIKVDVDANQDLAAEFGVQGVPTLVVFVDGEVAEQVVGAQSEDRLRELVDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYH7</accession>
    <name>A0A1U7EYH7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">SNF family transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tp55</name>
        <name evidence="7" type="ordered locus">NP_4362A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323888.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYH7"/>
    <dbReference type="STRING" id="348780.NP_4362A"/>
    <dbReference type="EnsemblBacteria" id="CAI50272">
        <property type="protein sequence ID" value="CAI50272"/>
        <property type="gene ID" value="NP_4362A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703344"/>
    <dbReference type="KEGG" id="nph:NP_4362A"/>
    <dbReference type="eggNOG" id="arCOG04466">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006855_3_4_2"/>
    <dbReference type="OrthoDB" id="99721at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd10336">
        <property type="entry name" value="SLC6sbd_Tyt1-Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047218">
        <property type="entry name" value="YocR/YhdH-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037979">
        <property type="entry name" value="Na_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42948">
        <property type="entry name" value="TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42948:SF1">
        <property type="entry name" value="TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="85"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="132"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="209"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="299"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="337"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="370"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="408"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI50272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="442" mass="46826" checksum="384A4CF07582EDED" modified="2017-05-10" version="1">MERETWATRIGFILAAVGSAVGLGNIWRFPFQVGQNGGSAFLIMYLACVLLIGIPAILVEFVIGRRSQRNPIGAFHALGKSRFRAIGAVCVVTGFVILSYYTVVAGWVLRYIGGSATGAYFGSEEAYFLEIATGYDAVVLHAVFLAVTVGIVMLGIKRGIELAVKAMVPAIALLLAGLAVYALTLDGVVDGYRYYLSPDFATLAAEWTTILPAAVGQALFTLSLGMGVMITYASYVGEDQSLLVDSGSIALLDTVIAFLSGLILFPILFTIDATPGDGGAGEFFIGVGGAFADIPLGTIVGVVFFVTLAVAALSSAISILEVIVSYFIDEFGFDRRVATFGVGVIVFVAGIPAALDMGTFEVYDQVTGELLLPLGMFLLVLFVAWVVPEESLDELLKGRSGSNLDVFWLWWVRTVVTVAVGLTLVLSVYGFAVEFGLLAESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYJ8</accession>
    <name>A0A1U7EYJ8_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aminotransferase</fullName>
            <ecNumber evidence="7">2.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">aspC3</name>
        <name evidence="9" type="ordered locus">NP_4410A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323911.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYJ8"/>
    <dbReference type="STRING" id="348780.NP_4410A"/>
    <dbReference type="EnsemblBacteria" id="CAI50296">
        <property type="protein sequence ID" value="CAI50296"/>
        <property type="gene ID" value="NP_4410A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702535"/>
    <dbReference type="KEGG" id="nph:NP_4410A"/>
    <dbReference type="eggNOG" id="arCOG01130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_4_3_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000033">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050596">
        <property type="entry name" value="AspAT/PAT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383:SF1">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 9" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="8">
        <location>
            <begin position="33"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI50296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="382" mass="40810" checksum="46BC45A5766AFE23" modified="2017-05-10" version="1">MTMDFTARLERVEPSATLAISNLASELEADGVDVVDLSVGEPDFPTPENIVEAGKDALDAGHTGYPPSNGIPKLKEAIADKLDADGLDYSTDEVIVTPGAKQALYETFQALVEDGDEVVLLDPAWVSYEAMAKMAGGDLSRVDLSGSSFQLEPALEDLAATVSDETELLVINSPSNPSGAVFSDAALEGVRDLAVDHDITVISDEIYDAITYDTEQTSLGSLEGMADRTVTINGFSKAYSMTGWRLGYLAAPEPLVEQAGKLHSHSVSSAANFVQHAGVEALRNTDDAVDEMRDAFEARRDMLIDLFADHGKEVPFPDGAFYMMLPVDDDDQAWCEGALEDAHVATVPGSAFGTPGYARLSYAASEERLKTGVERLVDAGYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYL4</accession>
    <name>A0A1U7EYL4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Flavin-dependent pyridine nucleotide oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">NP_4516A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
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        <property type="protein sequence ID" value="CAI50349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323964.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYL4"/>
    <dbReference type="STRING" id="348780.NP_4516A"/>
    <dbReference type="EnsemblBacteria" id="CAI50349">
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        <property type="gene ID" value="NP_4516A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702086"/>
    <dbReference type="KEGG" id="nph:NP_4516A"/>
    <dbReference type="eggNOG" id="arCOG01070">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003291_4_4_2"/>
    <dbReference type="OrthoDB" id="28009at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="FAD-bd_OxRdtase"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR041575">
        <property type="entry name" value="Rubredoxin_C"/>
    </dbReference>
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        <property type="entry name" value="NITRITE REDUCTASE [NAD(P)H]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43429">
        <property type="entry name" value="PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASE DOMAIN-CONTAINING"/>
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    </dbReference>
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        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
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    </dbReference>
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    <feature type="domain" description="FAD/NAD(P)-binding" evidence="4">
        <location>
            <begin position="4"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="NADH-rubredoxin oxidoreductase C-terminal" evidence="5">
        <location>
            <begin position="329"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF18267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI50349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="414" mass="44780" checksum="A2FD76EDF4A9F504" modified="2017-05-10" version="1">MCASHVIIGDGIAGSSAAETIREEDPDADITVITEEGEALYNRILIKEFAKGKLPEAPVSIHEPEWYEERDIDLRLNTVVTGVDVDAHEVYTHDGETVAYDKLLVATGGTPTQLPVDNSDAEGVNHFWTFEDARAIKENAESADSGVVVGAGLLGIDLAAICGAQGLDAHYLMRGEAWWRYALSQDGAEIIHDALEEMGVTPVFDSGVDHFETDDDGRVTAAVDPNGDRFEAEFAGVAIGLNFNTEFLQGTGIETDNGIVTDEHMRTNVDDVYAAGDITQFHDVIVGDRAQNGAWGSAKEQGNIAGQNMVADGPEATFEWVSSYSITHFDFPFLSFGHPTRGDETVEKKYDDETWRRVALKDGRVIGGVLIGDLSAQSVLKKLARKQIDVSDQTDLLLEEGVDIEAFEGAATAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ06</accession>
    <name>A0A1U7EZ06_NATPD</name>
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        <submittedName>
            <fullName evidence="2">DUF88 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4776A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50479.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ06"/>
    <dbReference type="STRING" id="348780.NP_4776A"/>
    <dbReference type="EnsemblBacteria" id="CAI50479">
        <property type="protein sequence ID" value="CAI50479"/>
        <property type="gene ID" value="NP_4776A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702171"/>
    <dbReference type="KEGG" id="nph:NP_4776A"/>
    <dbReference type="eggNOG" id="arCOG02408">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100862_1_0_2"/>
    <dbReference type="OrthoDB" id="85234at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NYN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35811:SF1">
        <property type="entry name" value="HTH OST-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35811">
        <property type="entry name" value="SLR1870 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01936">
        <property type="entry name" value="NYN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NYN" evidence="1">
        <location>
            <begin position="12"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50479.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="145" mass="15391" checksum="955F45841A07F36B" modified="2017-05-10" version="1">MGLFDRLRGETRVGIFVDGPNVFRAEFDVDLDELRALAGDEGTIAVARVYVDENASGGLIQAAEARGFEVVTTSGDVDVKLAVDAVEAAVDEQVDTVVVVSRDTDFKPVLETAAKRGLHTVAVAPGTHGRSDALRNAAHDEITLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ25</accession>
    <name>A0A1U7EZ25_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_4818A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
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            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324112.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ25"/>
    <dbReference type="STRING" id="348780.NP_4818A"/>
    <dbReference type="EnsemblBacteria" id="CAI50500">
        <property type="protein sequence ID" value="CAI50500"/>
        <property type="gene ID" value="NP_4818A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702186"/>
    <dbReference type="KEGG" id="nph:NP_4818A"/>
    <dbReference type="eggNOG" id="arCOG06373">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_171555_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058283">
        <property type="entry name" value="DUF7977"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25932">
        <property type="entry name" value="DUF7977"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="112" mass="12398" checksum="B09940A4C6D1EE8A" modified="2017-05-10" version="1">MSDGYRHDPDAFDEEGDRIVESDIEESRGHGGSGGDDVSGEHPDAVEREFDWRGWTLVAAIFVAFLVVPTIIYLYPRFPTLFGLSFLDAYLVLPMVPALVLGVLAVWATTRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ37</accession>
    <name>A0A1U7EZ37_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4920A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324163.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ37"/>
    <dbReference type="STRING" id="348780.NP_4920A"/>
    <dbReference type="EnsemblBacteria" id="CAI50551">
        <property type="protein sequence ID" value="CAI50551"/>
        <property type="gene ID" value="NP_4920A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702200"/>
    <dbReference type="KEGG" id="nph:NP_4920A"/>
    <dbReference type="HOGENOM" id="CLU_2353293_0_0_2"/>
    <dbReference type="OrthoDB" id="383706at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="96" mass="10332" checksum="18482E1CCA3948C8" modified="2017-05-10" version="1">MQRVTEIIDTMHDANSGSSIRVETEADVYHGTVTETEYTPPGADETGHVRVEFQTAADETFVVRTAASDAARKFSRPEVQPATGGEAAPVGAFDFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZE2</accession>
    <name>A0A1U7EZE2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Inorganic pyrophosphatase</fullName>
            <ecNumber evidence="7">3.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Pyrophosphate phospho-hydrolase</fullName>
            <shortName evidence="7">PPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ipp</name>
        <name evidence="7" type="synonym">ppa</name>
        <name evidence="8" type="ordered locus">NP_5192A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the hydrolysis of inorganic pyrophosphate (PPi) forming two phosphate ions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>diphosphate + H2O = 2 phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24576"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="EC" id="3.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.1" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324297.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZE2"/>
    <dbReference type="STRING" id="348780.NP_5192A"/>
    <dbReference type="EnsemblBacteria" id="CAI50687">
        <property type="protein sequence ID" value="CAI50687"/>
        <property type="gene ID" value="NP_5192A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702872"/>
    <dbReference type="KEGG" id="nph:NP_5192A"/>
    <dbReference type="eggNOG" id="arCOG01711">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073198_1_2_2"/>
    <dbReference type="OrthoDB" id="134160at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006796">
        <property type="term" value="P:phosphate-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00412">
        <property type="entry name" value="pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.80.10:FF:000003">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.80.10">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00209">
        <property type="entry name" value="Inorganic_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008162">
        <property type="entry name" value="Pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036649">
        <property type="entry name" value="Pyrophosphatase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10286">
        <property type="entry name" value="INORGANIC PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00719">
        <property type="entry name" value="Pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50324">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI50687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="179" mass="20628" checksum="44DF807AC1860735" modified="2017-05-10" version="1">MAGANLWEDLETGPNAPEEIYAVVECLKGERNKYEYDKDIPGVMLDRVLHSNVHYPGDYGFIPQSYYDDEDPFDVLVLVEDQTFPGCIIEARPVALMKMDDDGEQDDKVIAVPSEDPRYDHIEDLEDIPQQTLDEIDEFFETYKNLEEGKEVETQGWEDKQAAFDAIEHAQDLYEEHFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZL8</accession>
    <name>A0A1U7EZL8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Adenosylcobinamide-phosphate guanylyltransferase</fullName>
            <ecNumber evidence="2">2.7.7.62</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cobY</name>
        <name evidence="2" type="ordered locus">NP_5304A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.62" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50743.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZL8"/>
    <dbReference type="STRING" id="348780.NP_5304A"/>
    <dbReference type="EnsemblBacteria" id="CAI50743">
        <property type="protein sequence ID" value="CAI50743"/>
        <property type="gene ID" value="NP_5304A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_5304A"/>
    <dbReference type="eggNOG" id="arCOG01871">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098907_0_0_2"/>
    <dbReference type="OrthoDB" id="9782at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008820">
        <property type="term" value="F:cobinamide phosphate guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43777">
        <property type="entry name" value="MOLYBDENUM COFACTOR CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43777:SF1">
        <property type="entry name" value="MOLYBDENUM COFACTOR CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MobA-like NTP transferase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50743.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="192" mass="19810" checksum="A90591CE140C42DD" modified="2017-05-10" version="1">MCGGRGSRLDRGEKPLVAVDGVPMVDRVIDAVTASGFDTVAAVTSPHAPDTAAHLDGRVPLIETDGDGYVADLTDAIDRVGTPVVTVAADVPLLAPGPLDRLREAYEGPSVTLCVPSALKRQLGVSVDTERDHEGVAVAPTGVNVVGADDAETVRVSHDARLAVNVNRPDDLAIADRLAAGLPREVSSDGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZM4</accession>
    <name>A0A1U7EZM4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Homolog to phage PhiH1 repressor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_5364A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324381.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZM4"/>
    <dbReference type="STRING" id="348780.NP_5364A"/>
    <dbReference type="EnsemblBacteria" id="CAI50773">
        <property type="protein sequence ID" value="CAI50773"/>
        <property type="gene ID" value="NP_5364A"/>
    </dbReference>
    <dbReference type="GeneID" id="95969900"/>
    <dbReference type="KEGG" id="nph:NP_5364A"/>
    <dbReference type="eggNOG" id="arCOG03924">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161782_1_0_2"/>
    <dbReference type="OrthoDB" id="285635at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="104" mass="11725" checksum="C7F310CA478E1129" modified="2017-05-10" version="1">MRQPGTWMTIWDDRILEHIRAEDGGRVGELADKENIRVTNTHVSRRCKKLAEHGLLRDLGNGVYVITEEGEAYLDGEYDAENHVYLNDNETESGPTAGEEPGEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZN9</accession>
    <name>A0A1U7EZN9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_5344A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324372.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZN9"/>
    <dbReference type="STRING" id="348780.NP_5344A"/>
    <dbReference type="EnsemblBacteria" id="CAI50763">
        <property type="protein sequence ID" value="CAI50763"/>
        <property type="gene ID" value="NP_5344A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702342"/>
    <dbReference type="KEGG" id="nph:NP_5344A"/>
    <dbReference type="eggNOG" id="arCOG08168">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_564554_0_0_2"/>
    <dbReference type="OrthoDB" id="320276at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058376">
        <property type="entry name" value="DUF8063"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26259">
        <property type="entry name" value="DUF8063"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="145"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="207"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="426"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="444"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="458"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="468" mass="49952" checksum="FD5054FC24C073EA" modified="2017-05-10" version="1">MKRITTVLLVFVAVVGCLTAPAAAQAETPTPTPDGDEAQTIEHQLGNSDVLEVDWTDRTAHITIEADSRARVAITDAGAIDNLGSGGSERIGFETYTIPPGETREIQFTTASDPVITIQEGGEMVAASGDRQVLDIITGAPTVALVQWAAVSGISGTLLSTGLSVGLLKRRHQNSYRELTNDERHRIERDPVDGVVGRAKRFLADHYVAIALAVVSGVYALLMAFGVVRGPLAFWGDLADAHRVIIAGTAATTVVALPPAFALVSRVWSPDVEFVLDIDVDDIIQSARGVEPEDGGFAIYSGPPERIRQMDLRNADTDAGKSKAETPGGRAVVVRNMDPTDNEADGTWPGLADDRELVADQAKIRDNRNRLLDSAELGKNLLREISGISIASDIASTRAVDKAVRSALSTDSGAIDDILDEAAEGTRWEDYYTPGEAAEDDENADHANESPDERADITNDDDTTEDST</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILS8</accession>
    <name>Q3ILS8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3328A</name>
        <name evidence="3" type="ordered locus">NP_7052A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>PL23</name>
    </geneLocation>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
            <strain evidence="3">Gabara</strain>
            <plasmid evidence="3">PL23</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CR936259">
        <property type="protein sequence ID" value="CAI50942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILS8"/>
    <dbReference type="EnsemblBacteria" id="CAI49755">
        <property type="protein sequence ID" value="CAI49755"/>
        <property type="gene ID" value="NP_3328A"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI50942">
        <property type="protein sequence ID" value="CAI50942"/>
        <property type="gene ID" value="NP_7052A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3328A"/>
    <dbReference type="KEGG" id="nph:NP_7052A"/>
    <dbReference type="HOGENOM" id="CLU_1901995_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="133" mass="14713" checksum="70FDC81F66C9924A" modified="2005-11-08" version="1">MAATPTVIVGGPTQLIKNRCWNRDRCVTDLHFVCFVWCGCGSFSAKAGGLSSLCALKHTPVGPPAHEPQNIRRRRSAERLSKAVAAPRSRRTPGWRAVSLIKRRSEEQCCHSEGPRQWRASTSSFPSSFVRPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILT0</accession>
    <name>Q3ILT0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3324A</name>
        <name evidence="3" type="ordered locus">NP_7048A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>PL23</name>
    </geneLocation>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
            <strain evidence="3">Gabara</strain>
            <plasmid evidence="3">PL23</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CR936259">
        <property type="protein sequence ID" value="CAI50940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323374.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILT0"/>
    <dbReference type="STRING" id="348780.NP_3324A"/>
    <dbReference type="EnsemblBacteria" id="CAI49753">
        <property type="protein sequence ID" value="CAI49753"/>
        <property type="gene ID" value="NP_3324A"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI50940">
        <property type="protein sequence ID" value="CAI50940"/>
        <property type="gene ID" value="NP_7048A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703484"/>
    <dbReference type="KEGG" id="nph:NP_3324A"/>
    <dbReference type="KEGG" id="nph:NP_7048A"/>
    <dbReference type="HOGENOM" id="CLU_2748303_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="70" mass="7705" checksum="BDB50BAEC62629EB" modified="2005-11-08" version="1">MFETVVPKLRKWVRLLAFFGGLAAVGFGIYSILVEPLFLVPGPAEPTRGIEAELLLIVGGFMWCLLVTKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILV3</accession>
    <name>Q3ILV3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3278A</name>
        <name evidence="2" type="ordered locus">NP_7002A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>PL23</name>
    </geneLocation>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
            <strain evidence="2">Gabara</strain>
            <plasmid evidence="2">PL23</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CR936259">
        <property type="protein sequence ID" value="CAI50917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323351.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILV3"/>
    <dbReference type="EnsemblBacteria" id="CAI49730">
        <property type="protein sequence ID" value="CAI49730"/>
        <property type="gene ID" value="NP_3278A"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI50917">
        <property type="protein sequence ID" value="CAI50917"/>
        <property type="gene ID" value="NP_7002A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703461"/>
    <dbReference type="KEGG" id="nph:NP_3278A"/>
    <dbReference type="KEGG" id="nph:NP_7002A"/>
    <dbReference type="eggNOG" id="arCOG06318">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2504842_0_0_2"/>
    <dbReference type="OrthoDB" id="260010at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055810">
        <property type="entry name" value="DUF7386"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24111">
        <property type="entry name" value="DUF7386"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="86" mass="9905" checksum="CC06FE7CFF9F1464" modified="2005-11-08" version="1">MGERTTLRLSDERKHLLDQASDIVASDPSDDPPRSDVIDAALTHLIESHENLEDVRDKYPPGEVKDCCNTSVLQLRYRTSIEQRWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILX5</accession>
    <name>Q3ILX5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_6230A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILX5"/>
    <dbReference type="EnsemblBacteria" id="CAI50895">
        <property type="protein sequence ID" value="CAI50895"/>
        <property type="gene ID" value="NP_6230A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_6230A"/>
    <dbReference type="HOGENOM" id="CLU_2103577_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="22"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="115" mass="12682" checksum="724EECDB2CA767DA" modified="2005-11-08" version="1">MSEDTTTDNDGKRLGTRFRPDTIGHVFGTMAAVGGGVFLLSFFSVFGYAMLANPPDGAPLPLPITIAGIGPWMCVTAGLWGYVVWGSWTLRNHRQEPRRSEREVISNSDNRNLQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM12</accession>
    <name>Q3IM12_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_6152A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324455.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM12"/>
    <dbReference type="EnsemblBacteria" id="CAI50856">
        <property type="protein sequence ID" value="CAI50856"/>
        <property type="gene ID" value="NP_6152A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694628"/>
    <dbReference type="KEGG" id="nph:NP_6152A"/>
    <dbReference type="HOGENOM" id="CLU_2216956_0_0_2"/>
    <dbReference type="OrthoDB" id="203402at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058378">
        <property type="entry name" value="DUF8065"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26261">
        <property type="entry name" value="DUF8065"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="108" mass="12071" checksum="BCC4C744F06E6C65" modified="2005-11-08" version="1">MSGSSYDKTIQFRAGAEKEAAELLDEIHIGGVNVSELARVGLVEMLRQSLDEHDKIAVYERYSRGEIGEDVARVLLGEKLDRMEEEKESFEAAMERDTSEFLTGSDGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMP6</accession>
    <name>TRUA_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA pseudouridine synthase A</fullName>
            <ecNumber evidence="1">5.4.99.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA pseudouridine(38-40) synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA pseudouridylate synthase I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA-uridine isomerase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">truA</name>
        <name type="ordered locus">NP_5042A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:22376"/>
            <dbReference type="Rhea" id="RHEA-COMP:10085"/>
            <dbReference type="Rhea" id="RHEA-COMP:10087"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tRNA pseudouridine synthase TruA family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.12" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324222.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMP6"/>
    <dbReference type="SMR" id="Q3IMP6"/>
    <dbReference type="STRING" id="348780.NP_5042A"/>
    <dbReference type="EnsemblBacteria" id="CAI50612">
        <property type="protein sequence ID" value="CAI50612"/>
        <property type="gene ID" value="NP_5042A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703420"/>
    <dbReference type="KEGG" id="nph:NP_5042A"/>
    <dbReference type="eggNOG" id="arCOG04449">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014673_4_2_2"/>
    <dbReference type="OrthoDB" id="25720at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160147">
        <property type="term" value="F:tRNA pseudouridine(38-40) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.660">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00171">
        <property type="entry name" value="TruA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020097">
        <property type="entry name" value="PsdUridine_synth_TruA_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020095">
        <property type="entry name" value="PsdUridine_synth_TruA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000622">
        <property type="entry name" value="PRK00021.3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142:SF0">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01416">
        <property type="entry name" value="PseudoU_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001430">
        <property type="entry name" value="tRNA_psdUrid_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_1000017120" description="tRNA pseudouridine synthase A">
        <location>
            <begin position="1"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00171"/>
        </source>
    </evidence>
    <sequence length="267" mass="28635" checksum="33D0DD5BD95263ED" modified="2005-11-08" version="1">MRAFRIAYDGTGFRGFQRQPHGETVENALFEALSSLGVAFEDGRPPGYAAAGRTDAGVSARAQTVAFEAPAWLLPRALNGELPASVRAWAAADVGDAFHATHDATARAYRYFLYAPEPSADTTRARAACARLSGSHDFHNFTPDDRGTERTLSMRLRRQGPFLVVDCRAGGFARQLVRRLVAAVAAVARGERPLSFLGRALDDDPLSGADGIAAAPPEPLLLADVVYPGVEFTVDDRAAESARTVFATRRRQRLADARVAGALSPPE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-21" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMS5</accession>
    <name>IF2P_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Probable translation initiation factor IF-2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">infB</name>
        <name type="ordered locus">NP_4982A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324193.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMS5"/>
    <dbReference type="SMR" id="Q3IMS5"/>
    <dbReference type="STRING" id="348780.NP_4982A"/>
    <dbReference type="EnsemblBacteria" id="CAI50582">
        <property type="protein sequence ID" value="CAI50582"/>
        <property type="gene ID" value="NP_4982A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703271"/>
    <dbReference type="KEGG" id="nph:NP_4982A"/>
    <dbReference type="eggNOG" id="arCOG01560">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002656_3_3_2"/>
    <dbReference type="OrthoDB" id="30957at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03703">
        <property type="entry name" value="aeIF5B_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16266">
        <property type="entry name" value="IF2_aeIF5B_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01887">
        <property type="entry name" value="IF2_eIF5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000112">
        <property type="entry name" value="Eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000013">
        <property type="entry name" value="eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10050:FF:000001">
        <property type="entry name" value="Translation initiation factor IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10050">
        <property type="entry name" value="Translation initiation factor IF- 2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00100_A">
        <property type="entry name" value="IF_2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029459">
        <property type="entry name" value="EFTU-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004544">
        <property type="entry name" value="TF_aIF-2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023115">
        <property type="entry name" value="TIF_IF2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036925">
        <property type="entry name" value="TIF_IF2_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00491">
        <property type="entry name" value="aIF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003078">
        <property type="entry name" value="PRK04004.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381:SF4">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14578">
        <property type="entry name" value="GTP_EFTU_D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11987">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52156">
        <property type="entry name" value="Initiation factor IF2/eIF5b, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000228267" description="Probable translation initiation factor IF-2">
        <location>
            <begin position="1"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="domain" description="tr-type G">
        <location>
            <begin position="15"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="G1" evidence="1">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="G2" evidence="1">
        <location>
            <begin position="49"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="G3" evidence="1">
        <location>
            <begin position="86"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="G4" evidence="1">
        <location>
            <begin position="140"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="G5" evidence="1">
        <location>
            <begin position="208"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="86"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="140"/>
            <end position="143"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00100"/>
        </source>
    </evidence>
    <sequence length="602" mass="65542" checksum="1D2433DC192017EC" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMX4</accession>
    <name>RS14Z_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein uS14A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">30S ribosomal protein S14 type Z 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps14a</name>
        <name evidence="1" type="synonym">rps141</name>
        <name type="ordered locus">NP_4882A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds 16S rRNA, required for the assembly of 30S particles.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS14 family. Zinc-binding uS14 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324144.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMX4"/>
    <dbReference type="SMR" id="Q3IMX4"/>
    <dbReference type="STRING" id="348780.NP_4882A"/>
    <dbReference type="EnsemblBacteria" id="CAI50532">
        <property type="protein sequence ID" value="CAI50532"/>
        <property type="gene ID" value="NP_4882A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703169"/>
    <dbReference type="KEGG" id="nph:NP_4882A"/>
    <dbReference type="eggNOG" id="arCOG00782">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_177289_2_1_2"/>
    <dbReference type="OrthoDB" id="5615at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.830.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.10">
        <property type="entry name" value="30s Ribosomal Protein S14, Chain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01364_A">
        <property type="entry name" value="Ribosomal_uS14_2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001209">
        <property type="entry name" value="Ribosomal_uS14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023676">
        <property type="entry name" value="Ribosomal_uS14_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018271">
        <property type="entry name" value="Ribosomal_uS14_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039744">
        <property type="entry name" value="RIbosomal_uS14_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043140">
        <property type="entry name" value="Ribosomal_uS14_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004424">
        <property type="entry name" value="PRK05766.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12010">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12010:SF2">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00253">
        <property type="entry name" value="Ribosomal_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00527">
        <property type="entry name" value="RIBOSOMAL_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000269168" description="Small ribosomal subunit protein uS14A">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="56" mass="6421" checksum="3FDAE3848E2084D1" modified="2005-11-08" version="1">MSDSEQTGEQAAKRTGQLETCQRCGRKQGLVGKYDIWLCRQCFREVARGMGFKKYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IND3</accession>
    <name>Q3IND3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Probable NAD kinase (Polyphosphate/ATP)</fullName>
            <ecNumber evidence="1">2.7.1.23</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nadK2</name>
        <name evidence="1" type="ordered locus">NP_4558A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.1.23" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323985.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IND3"/>
    <dbReference type="STRING" id="348780.NP_4558A"/>
    <dbReference type="EnsemblBacteria" id="CAI50370">
        <property type="protein sequence ID" value="CAI50370"/>
        <property type="gene ID" value="NP_4558A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703047"/>
    <dbReference type="KEGG" id="nph:NP_4558A"/>
    <dbReference type="eggNOG" id="arCOG01348">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008831_2_0_2"/>
    <dbReference type="OrthoDB" id="170401at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275:SF43">
        <property type="entry name" value="BIFUNCTIONAL NADP PHOSPHATASE_NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20143">
        <property type="entry name" value="NAD_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="238" mass="24303" checksum="0789A6BE17856652" modified="2005-11-08" version="1">MSGQRVGVFGETGLEAAIEAAGGEPVDGEATDVAFVVAAGDRAVSDIAAEAGPEAAPVLPVGETKGIRAVPQDRLEQAVADALAGTARTERHPVVTAAAGEETTTAVFDVALMAAEPAQISEFSVRTQETAVSQFRADGLVVSTPAGSTGYGRRAGGPTVAAETGVVSVVPVAPFSTASGQWVLPIESVTLTVERDETPVELLADGRREWTVGAATPLSFVVDGQFELLVVDGSRSRF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IQ37</accession>
    <name>TRPF_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-(5'-phosphoribosyl)anthranilate isomerase</fullName>
            <shortName evidence="1">PRAI</shortName>
            <ecNumber evidence="1">5.3.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trpF</name>
        <name type="ordered locus">NP_3340A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:21540"/>
            <dbReference type="ChEBI" id="CHEBI:18277"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="EC" id="5.3.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TrpF family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.24" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323381.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IQ37"/>
    <dbReference type="SMR" id="Q3IQ37"/>
    <dbReference type="STRING" id="348780.NP_3340A"/>
    <dbReference type="EnsemblBacteria" id="CAI49761">
        <property type="protein sequence ID" value="CAI49761"/>
        <property type="gene ID" value="NP_3340A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703416"/>
    <dbReference type="KEGG" id="nph:NP_3340A"/>
    <dbReference type="eggNOG" id="arCOG01983">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076364_2_1_2"/>
    <dbReference type="OrthoDB" id="27513at2157"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00042"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004640">
        <property type="term" value="F:phosphoribosylanthranilate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00405">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00135">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001240">
        <property type="entry name" value="PRAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044643">
        <property type="entry name" value="TrpF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42894">
        <property type="entry name" value="N-(5'-PHOSPHORIBOSYL)ANTHRANILATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42894:SF1">
        <property type="entry name" value="N-(5'-PHOSPHORIBOSYL)ANTHRANILATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00697">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="chain" id="PRO_1000018612" description="N-(5'-phosphoribosyl)anthranilate isomerase">
        <location>
            <begin position="1"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00135"/>
        </source>
    </evidence>
    <sequence length="208" mass="20745" checksum="414508A340FAE2D2" modified="2005-11-08" version="1">MTRVKVCGITNRSDLDTAVDAGVDAVGLIVDVDVQTPREISPQQAAELAAATPPFVTAVLVTMADVPDAATELVEAVRPDAVQVHGESTPDALASLGAAVEADVIKATDPGTAATYDGHADAVLVDSLDESGAGGTGTVHDWDRTGELVESLQSPVVLAGGLTPENVADAVEAAAPFAVDVASGVEAESGQKDADAVSAFVDAAGGCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IRA2</accession>
    <name>Q3IRA2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ubiquitin-like modifier protein SAMP3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">samp3</name>
        <name evidence="1" type="ordered locus">NP_2500A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322967.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IRA2"/>
    <dbReference type="STRING" id="348780.NP_2500A"/>
    <dbReference type="EnsemblBacteria" id="CAI49341">
        <property type="protein sequence ID" value="CAI49341"/>
        <property type="gene ID" value="NP_2500A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702927"/>
    <dbReference type="KEGG" id="nph:NP_2500A"/>
    <dbReference type="eggNOG" id="arCOG00536">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114601_1_2_2"/>
    <dbReference type="OrthoDB" id="134663at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd17505">
        <property type="entry name" value="Ubl_SAMP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010038">
        <property type="entry name" value="MoaD_arc-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054834">
        <property type="entry name" value="SAMP1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052045">
        <property type="entry name" value="Sulfur_Carrier/Prot_Modifier"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003749">
        <property type="entry name" value="ThiS/MoaD-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01687">
        <property type="entry name" value="moaD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041918">
        <property type="entry name" value="SAMP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38031:SF1">
        <property type="entry name" value="SULFUR CARRIER PROTEIN CYSO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38031">
        <property type="entry name" value="SULFUR CARRIER PROTEIN SLR0821-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02597">
        <property type="entry name" value="ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="MoaD/ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="93" mass="10042" checksum="998E1F4322E6AA18" modified="2005-11-08" version="1">MQLALRFFATFREAVGTKEIERTFDAEATVGDVLAAVEDEFPELAGDILDDDGDIQPHLTVLRNGREVVHLDGTATELSDGDRLSIFPPVAGG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-02-22" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IST1</accession>
    <name>HPRL_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HGPRTase-like protein</fullName>
            <ecNumber evidence="1">2.4.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">NP_1426A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May catalyze a purine salvage reaction, the substrate is unknown.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the purine/pyrimidine phosphoribosyltransferase family. Archaeal HPRT subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.-" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322439.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IST1"/>
    <dbReference type="SMR" id="Q3IST1"/>
    <dbReference type="STRING" id="348780.NP_1426A"/>
    <dbReference type="EnsemblBacteria" id="CAI48804">
        <property type="protein sequence ID" value="CAI48804"/>
        <property type="gene ID" value="NP_1426A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702512"/>
    <dbReference type="KEGG" id="nph:NP_1426A"/>
    <dbReference type="eggNOG" id="arCOG00030">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126376_0_0_2"/>
    <dbReference type="OrthoDB" id="8323at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01467">
        <property type="entry name" value="Hypx_phosphoribosyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026597">
        <property type="entry name" value="HGPRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050118">
        <property type="entry name" value="Pur/Pyrimidine_PRTase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040646">
        <property type="entry name" value="HPT_Archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002635">
        <property type="entry name" value="PRK02304.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43864">
        <property type="entry name" value="HYPOXANTHINE/GUANINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43864:SF1">
        <property type="entry name" value="XANTHINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00103">
        <property type="entry name" value="PUR_PYR_PR_TRANSFER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0660">Purine salvage</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000415490" description="HGPRTase-like protein">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01467"/>
        </source>
    </evidence>
    <sequence length="189" mass="20539" checksum="91D1A0F69896D735" modified="2005-11-08" version="1">MDQLQQSLLEAPIIEKDGYHYFVHPISDGVPMLEPNLLREIVIKIIRKAQLENVDKIVTPAAMGIHISTAVSLMTDIPLVVIRKREYGLDGETPLFQQTGYSENQMYINDVDEGDSVLVLDDVLSTGGTLSAICSALEDIGADIVDVVAVIKKVGGENELESSPYSAKTLINVDVEDGEVVIIDSNGDN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-26" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IT15</accession>
    <name>PYRE_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Orotate phosphoribosyltransferase</fullName>
            <shortName evidence="1">OPRT</shortName>
            <shortName evidence="1">OPRTase</shortName>
            <ecNumber evidence="1">2.4.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrE</name>
        <name type="ordered locus">NP_1256A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate</text>
            <dbReference type="Rhea" id="RHEA:10380"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.10" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322355.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IT15"/>
    <dbReference type="SMR" id="Q3IT15"/>
    <dbReference type="STRING" id="348780.NP_1256A"/>
    <dbReference type="EnsemblBacteria" id="CAI48719">
        <property type="protein sequence ID" value="CAI48719"/>
        <property type="gene ID" value="NP_1256A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703084"/>
    <dbReference type="KEGG" id="nph:NP_1256A"/>
    <dbReference type="eggNOG" id="arCOG00029">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074878_2_0_2"/>
    <dbReference type="OrthoDB" id="9089at2157"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00119"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004588">
        <property type="term" value="F:orotate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019856">
        <property type="term" value="P:pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01208">
        <property type="entry name" value="PyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023031">
        <property type="entry name" value="OPRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004467">
        <property type="entry name" value="Or_phspho_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00336">
        <property type="entry name" value="pyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19278">
        <property type="entry name" value="OROTATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19278:SF9">
        <property type="entry name" value="URIDINE 5'-MONOPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000073108" description="Orotate phosphoribosyltransferase">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="113"/>
            <end position="121"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>orotate</name>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>orotate</name>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01208"/>
        </source>
    </evidence>
    <sequence length="173" mass="18547" checksum="EA05A98CF8C915E4" modified="2005-11-08" version="1">MANQELIDALRDAEAVKFGEFELSHGGTSEYYVDKYVFETDPGCLRQIADAFADRVGDAKLAGVALGAVPLVAATAVETDSPYVIVRKQKKEYGTGNQIEGDFDAGEEVVVLEDIATTGQSAIDAVEALREAGATVDRVIVVVDREEGARENLAEHDIELESLVTASDLLADR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ITS8</accession>
    <name>Q3ITS8_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphoesterase</fullName>
            <ecNumber evidence="3">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_0728A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallophosphoesterase superfamily. YfcE family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322091.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ITS8"/>
    <dbReference type="STRING" id="348780.NP_0728A"/>
    <dbReference type="EnsemblBacteria" id="CAI48455">
        <property type="protein sequence ID" value="CAI48455"/>
        <property type="gene ID" value="NP_0728A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700946"/>
    <dbReference type="KEGG" id="nph:NP_0728A"/>
    <dbReference type="eggNOG" id="arCOG01143">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074761_1_1_2"/>
    <dbReference type="OrthoDB" id="9937at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050126">
        <property type="entry name" value="Ap4A_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020935">
        <property type="entry name" value="PdiEstase_YfcE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011152">
        <property type="entry name" value="Pesterase_MJ0912"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000979">
        <property type="entry name" value="Phosphodiesterase_MJ0936/Vps29"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00040">
        <property type="entry name" value="yfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42850:SF2">
        <property type="entry name" value="BLL5683 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42850">
        <property type="entry name" value="METALLOPHOSPHOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000883">
        <property type="entry name" value="Pesterase_MJ0912"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01269">
        <property type="entry name" value="UPF0025"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="4">
        <location>
            <begin position="1"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI48455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="219" mass="23779" checksum="D9613B0D3E65B24E" modified="2005-11-08" version="1">MLVGVVSDIHANRIALEAVLDDMPPVDGLVCAGDVVGYNPWPADCVVRLRERSVPTVQGNHDRAVVTGTGFGGNGMADAGVKYARGALDDSHIEWLRGLPETRRCFDGRLKLVHGHPDDPDRYTYPGLFSPELLGDEDVLVMGHTHVQAIEQYDEGIVLNPGSVGQPRDGDPRAAYALVDLDSMTAERRRVEYDIEAVIEAVEEAGLPEGTGTRLRKGR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-12-06" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ITY2</accession>
    <name>GUAAA_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GMP synthase [glutamine-hydrolyzing] subunit A</fullName>
            <ecNumber evidence="1">6.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glutamine amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">guaAA</name>
        <name type="ordered locus">NP_0620A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the synthesis of GMP from XMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>XMP + L-glutamine + ATP + H2O = GMP + L-glutamate + AMP + diphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B).</text>
    </comment>
    <dbReference type="EC" id="6.3.5.2" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322037.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ITY2"/>
    <dbReference type="SMR" id="Q3ITY2"/>
    <dbReference type="STRING" id="348780.NP_0620A"/>
    <dbReference type="MEROPS" id="C26.A31"/>
    <dbReference type="EnsemblBacteria" id="CAI48401">
        <property type="protein sequence ID" value="CAI48401"/>
        <property type="gene ID" value="NP_0620A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702780"/>
    <dbReference type="KEGG" id="nph:NP_0620A"/>
    <dbReference type="eggNOG" id="arCOG00087">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014340_1_4_2"/>
    <dbReference type="OrthoDB" id="10772at2157"/>
    <dbReference type="UniPathway" id="UPA00189">
        <property type="reaction ID" value="UER00296"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003921">
        <property type="term" value="F:GMP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01742">
        <property type="entry name" value="GATase1_GMP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000047">
        <property type="entry name" value="GMP synthase [glutamine-hydrolyzing] subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01510">
        <property type="entry name" value="GMP_synthase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004739">
        <property type="entry name" value="GMP_synth_GATase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023686">
        <property type="entry name" value="GMP_synthase_A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00888">
        <property type="entry name" value="guaA_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001975">
        <property type="entry name" value="PRK00758.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11922:SF2">
        <property type="entry name" value="GMP SYNTHASE [GLUTAMINE-HYDROLYZING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11922">
        <property type="entry name" value="GMP SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0332">GMP biosynthesis</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000140226" description="GMP synthase [glutamine-hydrolyzing] subunit A">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-1" evidence="1">
        <location>
            <begin position="3"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01510"/>
        </source>
    </evidence>
    <sequence length="184" mass="19991" checksum="733B73D89EA4EC4B" modified="2005-11-08" version="1">MTRIVVVDNHGQFTHLEHRALRDLGVDTELIDNDTPPEEIDADGIVLSGGPDMDRIGHSPAYLDLDIPVLGICLGMQLMAAELGGRVGAGDYGGYADVTVSIEDESDPLIGSLAPETRVWASHADEVKELPDGFERTATSEVCNIEAMSDTDRNRYGVQWHPEVAHTEAGEEVFENFRDICAGD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-12-20" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ITY7</accession>
    <name>SYI_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Isoleucine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Isoleucyl-tRNA synthetase</fullName>
            <shortName evidence="1">IleRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ileS</name>
        <name type="ordered locus">NP_0610A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Ile) + L-isoleucine + ATP = L-isoleucyl-tRNA(Ile) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:9666"/>
            <dbReference type="Rhea" id="RHEA-COMP:9695"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78528"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.5" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322032.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ITY7"/>
    <dbReference type="SMR" id="Q3ITY7"/>
    <dbReference type="STRING" id="348780.NP_0610A"/>
    <dbReference type="EnsemblBacteria" id="CAI48396">
        <property type="protein sequence ID" value="CAI48396"/>
        <property type="gene ID" value="NP_0610A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702863"/>
    <dbReference type="KEGG" id="nph:NP_0610A"/>
    <dbReference type="eggNOG" id="arCOG00807">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001493_1_1_2"/>
    <dbReference type="OrthoDB" id="30823at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004822">
        <property type="term" value="F:isoleucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006428">
        <property type="term" value="P:isoleucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07961">
        <property type="entry name" value="Anticodon_Ia_Ile_ABEc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000286">
        <property type="entry name" value="Isoleucine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="entry name" value="Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02003">
        <property type="entry name" value="Ile_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033709">
        <property type="entry name" value="Anticodon_Ile_ABEc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002301">
        <property type="entry name" value="Ile-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023586">
        <property type="entry name" value="Ile-tRNA-ligase_type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00392">
        <property type="entry name" value="ileS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42780:SF1">
        <property type="entry name" value="ISOLEUCINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42780">
        <property type="entry name" value="SOLEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19302">
        <property type="entry name" value="DUF5915"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00984">
        <property type="entry name" value="TRNASYNTHILE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000098585" description="Isoleucine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="604"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="607"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02003"/>
        </source>
    </evidence>
    <sequence length="1061" mass="120589" checksum="2B24147A2FCB2EA2" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-05-20" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IU46</accession>
    <name>HELS_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent DNA helicase Hel308</fullName>
            <ecNumber evidence="1">5.6.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA 3'-5' helicase Hel308</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hel308</name>
        <name type="ordered locus">NP_0492A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent ATPase and 3'-5' DNA helicase that may be involved in repair of stalled replication forks.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. Hel308 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321973.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IU46"/>
    <dbReference type="SMR" id="Q3IU46"/>
    <dbReference type="STRING" id="348780.NP_0492A"/>
    <dbReference type="EnsemblBacteria" id="CAI48337">
        <property type="protein sequence ID" value="CAI48337"/>
        <property type="gene ID" value="NP_0492A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702913"/>
    <dbReference type="KEGG" id="nph:NP_0492A"/>
    <dbReference type="eggNOG" id="arCOG00553">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006553_3_0_2"/>
    <dbReference type="OrthoDB" id="371946at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18028">
        <property type="entry name" value="DEXHc_archSki2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00442">
        <property type="entry name" value="Helicase_Hel308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048772">
        <property type="entry name" value="Hel308-like_dom4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022965">
        <property type="entry name" value="Helicase_Hel308"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002654">
        <property type="entry name" value="PRK02362.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF10">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE HEL308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21280">
        <property type="entry name" value="Helicase_dom4_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14520">
        <property type="entry name" value="HHH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000080862" description="ATP-dependent DNA helicase Hel308">
        <location>
            <begin position="1"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="1">
        <location>
            <begin position="34"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="1">
        <location>
            <begin position="234"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="711"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DEAH box" evidence="1">
        <location>
            <begin position="145"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="47"/>
            <end position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="747" mass="81108" checksum="AE955549EC57148E" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETB5</accession>
    <name>A0A1U7ETB5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 19">Glutamine synthetase</fullName>
            <ecNumber evidence="3 19">6.3.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">glnA1</name>
        <name evidence="22" type="ordered locus">NP_0076A</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 19">
            <text>L-glutamate + NH4(+) + ATP = L-glutamine + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16169"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="14">Binds 2 Mg(2+) ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16 17">Belongs to the glutamine synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.2" evidence="3 19"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321768.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETB5"/>
    <dbReference type="STRING" id="348780.NP_0076A"/>
    <dbReference type="EnsemblBacteria" id="CAI48129">
        <property type="protein sequence ID" value="CAI48129"/>
        <property type="gene ID" value="NP_0076A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702754"/>
    <dbReference type="KEGG" id="nph:NP_0076A"/>
    <dbReference type="eggNOG" id="arCOG01909">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017290_1_3_2"/>
    <dbReference type="OrthoDB" id="36124at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamine synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.70:FF:000005">
        <property type="entry name" value="Glutamine synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000003">
        <property type="entry name" value="Glutamine synthetase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008147">
        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027303">
        <property type="entry name" value="Gln_synth_gly_rich_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004809">
        <property type="entry name" value="Gln_synth_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027302">
        <property type="entry name" value="Gln_synth_N_conserv_site"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00653">
        <property type="entry name" value="GlnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43407">
        <property type="entry name" value="GLUTAMINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43407:SF1">
        <property type="entry name" value="LENGSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03951">
        <property type="entry name" value="Gln-synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54368">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00180">
        <property type="entry name" value="GLNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00181">
        <property type="entry name" value="GLNA_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51986">
        <property type="entry name" value="GS_BETA_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51987">
        <property type="entry name" value="GS_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 18" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 19 22" id="KW-0436">Ligase</keyword>
    <keyword evidence="10 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 13 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GS beta-grasp" evidence="20">
        <location>
            <begin position="26"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="GS catalytic" evidence="21">
        <location>
            <begin position="120"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="210"/>
            <end position="212"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="251"/>
            <end position="252"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="258"/>
            <end position="260"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="345"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="347"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="O-AMP-tyrosine" evidence="15">
        <location>
            <position position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00049436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR604809-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR604809-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR604809-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR604809-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU004356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="CAI48129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="456" mass="50988" checksum="E2F8BEA6376F2B51" modified="2017-05-10" version="1">MTNDNVAADGGLTKEAQAVVDEIEEKNVDFLRLQFTDIIGTVKNVSVPADQAEKAFTEGIYFDGSSIEGFVRIQESDMRLIPDPETFAILPWRKREDGASARLICDVYNTSTGEPFEGDPRYVLKRAIDRAEEMGYSINFAPEPEFFLFEEDDEGRATTETADHGGYFDLAPKDLASDVRRDIIYGLESMDFEIEASHHEVARGQHEINFEYDDALTTADNVATFRTVVRAIAAQHDLHATFMPKPIPRINGSGMHTHMSLFEDGENAFHDEDDEFSLSGTARSYLAGILEHAPAITAVANPTVNSYKRLVPGYEAPVYVAWSDRNRSALVRKPAARIPAASRIELRSPDPSCNPYLAFAAMIHAGLDGIENDLDCPDPVRDNIYEFDEDTRDEYGIETLPSNLGEAVDALEADEVVLDALGEHISEKFVEAKTQEFEEYLVDVSEWELDRYLETF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETC6</accession>
    <name>A0A1U7ETC6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">DNA-directed RNA polymerase subunit beta</fullName>
            <ecNumber evidence="14">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">rpoB1</name>
        <name evidence="20" type="ordered locus">NP_0112A</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="12">Part of the RNA polymerase complex.</text>
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</entry>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
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        </lineage>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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        </source>
    </reference>
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    </dbReference>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104483_0_0_2"/>
    <dbReference type="OrthoDB" id="270259at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055687">
        <property type="entry name" value="DUF7263"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23924">
        <property type="entry name" value="DUF7263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="213" mass="22517" checksum="D004340C4B5B2A0B" modified="2017-05-10" version="1">MSRGQANLPALAIALLVLTTVAVLAVTIADASVRGAERNAADRAVATATADRLVAADSAATVRGNVLNGSLDADSVAASLPPDVDARVALDGEAVYERGDPTGGETARRIVLVADQQAVEVEPPFEFRSVTLPRRSPRATLDIEPSADIETVRANDRVVLYEPGGLDGTYEVPLSRYETTTLRVDGFPDDGEVTVTYYPRQTQKALLEVTVNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETH0</accession>
    <name>A0A1U7ETH0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DUF2267 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0214A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321837.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETH0"/>
    <dbReference type="STRING" id="348780.NP_0214A"/>
    <dbReference type="EnsemblBacteria" id="CAI48198">
        <property type="protein sequence ID" value="CAI48198"/>
        <property type="gene ID" value="NP_0214A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700791"/>
    <dbReference type="KEGG" id="nph:NP_0214A"/>
    <dbReference type="eggNOG" id="arCOG06189">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121888_3_0_2"/>
    <dbReference type="OrthoDB" id="212282at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.110">
        <property type="entry name" value="Uncharacterized conserved protein DUF2267"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018727">
        <property type="entry name" value="DUF2267"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038282">
        <property type="entry name" value="DUF2267_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10025">
        <property type="entry name" value="DUF2267"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="151" mass="16728" checksum="843FF400F7B83138" modified="2017-05-10" version="1">MSTSFKDFVGEVQHRIEAGEQAEAVRTTRAVLTTLGERVSEGGATDIASPLPKEIDRYLLAADHGQVYDYDTFIQRVTDRLNYDDLELDSGHGQPSNIDQGEAVYRTKAVVALVSDLVRGGELAHVEEQLPPEFDDLFEFSKTDSPPWGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETH7</accession>
    <name>A0A1U7ETH7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0242A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321851.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETH7"/>
    <dbReference type="EnsemblBacteria" id="CAI48212">
        <property type="protein sequence ID" value="CAI48212"/>
        <property type="gene ID" value="NP_0242A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700800"/>
    <dbReference type="KEGG" id="nph:NP_0242A"/>
    <dbReference type="eggNOG" id="arCOG07545">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1507428_0_0_2"/>
    <dbReference type="OrthoDB" id="185449at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046622">
        <property type="entry name" value="DUF6735"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20509">
        <property type="entry name" value="DUF6735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="178" mass="19420" checksum="950AE8481D5F8E01" modified="2017-05-10" version="1">MGHRTLVAYDRDGYDLHYAHWTPDPESLTVDTPFGRPPEDDWARKRADRLLDAEGGRLTEHRTTAVDSDPLATDLSFTDIGGYIDPLEHERLYVVDPSFSVTTYVVFGVENGRLSPPDAPTVALVGYDGERDAAYLRGWLAGGRAVRGVAGPDGASIVAALRWLDPDRGTVVWLPGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETH9</accession>
    <name>A0A1U7ETH9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Homolog to restriction system mrr</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0236A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321848.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETH9"/>
    <dbReference type="STRING" id="348780.NP_0236A"/>
    <dbReference type="EnsemblBacteria" id="CAI48209">
        <property type="protein sequence ID" value="CAI48209"/>
        <property type="gene ID" value="NP_0236A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700797"/>
    <dbReference type="KEGG" id="nph:NP_0236A"/>
    <dbReference type="eggNOG" id="arCOG02777">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_606365_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015666">
        <property type="term" value="F:restriction endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007560">
        <property type="entry name" value="Restrct_endonuc_IV_Mrr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052906">
        <property type="entry name" value="Type_IV_Methyl-Rstrct_Enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30015">
        <property type="entry name" value="MRR RESTRICTION SYSTEM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30015:SF7">
        <property type="entry name" value="TYPE IV METHYL-DIRECTED RESTRICTION ENZYME ECOKMRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04471">
        <property type="entry name" value="Mrr_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="420"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="Restriction endonuclease type IV Mrr" evidence="2">
        <location>
            <begin position="5"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="441" mass="48426" checksum="D7500B51600F539F" modified="2017-05-10" version="1">MTTLLDDLSGYEFEDAVASLFRALDYEDVSVATRVADEGRDITMYDGETAYVVECKHTDTVSRPVVQKLHSAVATYSHDGPKHGMVVTSGRFTGPAEKYAKELRERGDPHPIELLDGTDIRELGESVGMDLRNGRIEILCEETLPVGDPEHVLAAVFEEIKNAPPRTKLPEPAVEVGYNPIVDIEAVTRSTFETGAGVIHRINRRDRLIVEADPAGPRLAPGGVSTLTGIESVALSDEQKRHGGEAVRFGLTESEARDWTRERLCDRLETTVAYTGDNNVTYEQTCRPSPDDVALQQVEPCYLPRVDAAVELGAYTHRYEYDAAGERHVPRTDGIRRCVHCETAGADAATYTYCENCGSISCGTHTKTERLESEPVCTGCAVTGEFFYATKYFFDEENRAAFRDEYEAMPIHHKAMENPYLTAAIVAAALLVVAAVAVAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETJ8</accession>
    <name>A0A1U7ETJ8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">O-acetylhomoserine aminocarboxypropyltransferase (Methionine synthase)</fullName>
            <ecNumber evidence="5">2.5.1.49</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">metY1</name>
        <name evidence="5" type="ordered locus">NP_0284A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.49" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48233.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939390.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETJ8"/>
    <dbReference type="STRING" id="348780.NP_0284A"/>
    <dbReference type="EnsemblBacteria" id="CAI48233">
        <property type="protein sequence ID" value="CAI48233"/>
        <property type="gene ID" value="NP_0284A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702920"/>
    <dbReference type="KEGG" id="nph:NP_0284A"/>
    <dbReference type="eggNOG" id="arCOG00061">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018986_4_0_2"/>
    <dbReference type="OrthoDB" id="43458at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004124">
        <property type="term" value="F:cysteine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003961">
        <property type="term" value="F:O-acetylhomoserine aminocarboxypropyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071269">
        <property type="term" value="P:L-homocysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000035">
        <property type="entry name" value="O-succinylhomoserine sulfhydrylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006235">
        <property type="entry name" value="OAc-hSer/O-AcSer_sulfhydrylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01326">
        <property type="entry name" value="OAH_OAS_sulfhy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43797">
        <property type="entry name" value="HOMOCYSTEINE/CYSTEINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43797:SF2">
        <property type="entry name" value="HOMOCYSTEINE_CYSTEINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI48233.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="427" mass="45510" checksum="7CFDC2763E09CD54" modified="2017-05-10" version="1">MNDDERQLGFGTRCLHVGQGPDPETGAAAPPIYQTSSYEFPDADTAAARYALEDEGNVYSRVANPTVRALEERLASLEGGVDALATASGMGALDAATLVLAEAGDNIVSASSIYGGTHAYLSHTAARRGIESRFVETLDPAAYEEAIDEDTAYVHCETIGNPALVTPPLSEIAAVAHDNGVPLFVDNTFATPALCRPLEHGADLVWDSTTKWIHGSGTTLGGILVTDGSFPFEEYPEKYPEIAAPNPAFDGTVFAERFGDRAVTMAARHRATRSIGDPQSPFDAWATLQGSETLSLRMERHCENARAVAEFLADHPKVSWVTYPGLEAHETHDLANEYLDGGYGGMVAFGPEGGYEAGKRLCEDTEVAKFLANIGDARTLVIHPASTTHAQLTGEEQRASGVTPDMIRLSVGIEDVDDIIADLDATL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETL6</accession>
    <name>A0A1U7ETL6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UPF0148 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0338A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321898.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETL6"/>
    <dbReference type="STRING" id="348780.NP_0338A"/>
    <dbReference type="EnsemblBacteria" id="CAI48260">
        <property type="protein sequence ID" value="CAI48260"/>
        <property type="gene ID" value="NP_0338A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700822"/>
    <dbReference type="KEGG" id="nph:NP_0338A"/>
    <dbReference type="eggNOG" id="arCOG00578">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085607_0_0_2"/>
    <dbReference type="OrthoDB" id="26305at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009563">
        <property type="entry name" value="SSSCA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051888">
        <property type="entry name" value="UPF0148_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009142">
        <property type="entry name" value="PRK12495.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16537:SF1">
        <property type="entry name" value="PROTEIN ZNRD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16537">
        <property type="entry name" value="SJOEGREN SYNDROME/SCLERODERMA AUTOANTIGEN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06677">
        <property type="entry name" value="Auto_anti-p27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="77"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="244" mass="26398" checksum="915C7E49ACB9A39B" modified="2017-05-10" version="1">MSDFDREKEREKLREKYEQDKQKREASERMSELLLQGATMTNRHCPECHSPVFRHEGQEFCPTCQQVVTEDGDLVDPADAGAGADTGADSEAGSNASPDTDAQTDTDAEPAPSADAHEPSASEAAPERGRDPGADAPAEAEPEPDVDRRPATTDERRPARVDPPARSEPSEPSTPDAHESTGRRKAPRDRRPTGSGFDSTEATLADEIAALTQQAAETSDIGRKRDLLAAAREAAETLQTIRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETU5</accession>
    <name>A0A1U7ETU5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0514A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321984.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETU5"/>
    <dbReference type="STRING" id="348780.NP_0514A"/>
    <dbReference type="EnsemblBacteria" id="CAI48348">
        <property type="protein sequence ID" value="CAI48348"/>
        <property type="gene ID" value="NP_0514A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700880"/>
    <dbReference type="KEGG" id="nph:NP_0514A"/>
    <dbReference type="eggNOG" id="arCOG06403">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084116_0_0_2"/>
    <dbReference type="OrthoDB" id="212231at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046604">
        <property type="entry name" value="DUF6663"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20368">
        <property type="entry name" value="DUF6663"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="206" mass="22300" checksum="D1A71445F8D86BF3" modified="2017-05-10" version="1">MQGAPTAAVTDDERGRYRVLPGRNDDERQLLAVDGSEVVAVPATAVATADVGNVVAAGLGWETEPPAVRDVTVETATRFRFERTTEAMFEAARNCFERAQADGEAMNSRVTHGTDGDPNGVVYTFAEQPGEQDLFAEFRDGTKPLEPLVARAADGETPPFSVWVLDPEPPFVAVYIVLDPDGLLEQTMRETYLGDGGLAENIEQKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETX1</accession>
    <name>A0A1U7ETX1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Probable GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0584A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322019.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETX1"/>
    <dbReference type="STRING" id="348780.NP_0584A"/>
    <dbReference type="EnsemblBacteria" id="CAI48383">
        <property type="protein sequence ID" value="CAI48383"/>
        <property type="gene ID" value="NP_0584A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700897"/>
    <dbReference type="KEGG" id="nph:NP_0584A"/>
    <dbReference type="eggNOG" id="arCOG00352">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011784_0_0_2"/>
    <dbReference type="OrthoDB" id="147673at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01897">
        <property type="entry name" value="NOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041623">
        <property type="entry name" value="NOG1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45759">
        <property type="entry name" value="NUCLEOLAR GTP-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17835">
        <property type="entry name" value="NOG1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="2">
        <location>
            <begin position="163"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="327" mass="36257" checksum="4836FF8D75304823" modified="2017-05-10" version="1">MHFEDLPTTPRSEELVDKAFSRAARAGRAKQGKEAQESMLQTASNILSDNLENVVTSWPDFELVDTFYYEFADATLADTDVGGVDPLRQHLSEVMWAGRQADKIKDEYHGRIRNSDTDTARKLRKQAFARLADITEQVEDDLLAIGTAREALKTLPDIRADEPAIVVAGYPNVGKSSFVNTVTNARNETAAYPFTTTEINVGHVEDNHVRYQLIDTPGLLDRPSEDRNPIESQAVSALTHLADAVLFVLDASGECGYPLDSQRDLLDDIESTFDVPVIVVCNKADRSTDTEADHYMSVETGENVDEVLDAAIDAVDYELELPFEDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU10</accession>
    <name>A0A1U7EU10_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF5786 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0668A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322061.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU10"/>
    <dbReference type="STRING" id="348780.NP_0668A"/>
    <dbReference type="EnsemblBacteria" id="CAI48425">
        <property type="protein sequence ID" value="CAI48425"/>
        <property type="gene ID" value="NP_0668A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700923"/>
    <dbReference type="KEGG" id="nph:NP_0668A"/>
    <dbReference type="eggNOG" id="arCOG03012">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_209486_0_0_2"/>
    <dbReference type="OrthoDB" id="166327at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043902">
        <property type="entry name" value="DUF5786"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19099">
        <property type="entry name" value="DUF5786"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5786" evidence="2">
        <location>
            <begin position="3"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="61" mass="7012" checksum="D939660B91209E48" modified="2017-05-10" version="1">MGFGSYDESEQQDQNVETDEDAAVNVHENDHEGEVEFEFDENEDALIDRLQDMKDDDDDGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU46</accession>
    <name>A0A1U7EU46_NATPD</name>
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        <submittedName>
            <fullName evidence="7">ABC-type transport system ATP-binding protein (Probable substrate dipeptide/oligopeptide)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">dppF1</name>
        <name evidence="7" type="synonym">abc01a2</name>
        <name evidence="7" type="ordered locus">NP_0766A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322110.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU46"/>
    <dbReference type="STRING" id="348780.NP_0766A"/>
    <dbReference type="EnsemblBacteria" id="CAI48474">
        <property type="protein sequence ID" value="CAI48474"/>
        <property type="gene ID" value="NP_0766A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702363"/>
    <dbReference type="KEGG" id="nph:NP_0766A"/>
    <dbReference type="eggNOG" id="arCOG00184">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_2"/>
    <dbReference type="OrthoDB" id="18209at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03257">
        <property type="entry name" value="ABC_NikE_OppD_transporters"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050319">
        <property type="entry name" value="ABC_transp_ATP-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43776:SF7">
        <property type="entry name" value="D,D-DIPEPTIDE TRANSPORT ATP-BINDING PROTEIN DDPF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43776">
        <property type="entry name" value="TRANSPORT ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="28"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="389"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="529"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="393"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="551" mass="60304" checksum="A11040B2CBFEC904" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU94</accession>
    <name>A0A1U7EU94_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">NP_0862A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322158.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU94"/>
    <dbReference type="STRING" id="348780.NP_0862A"/>
    <dbReference type="EnsemblBacteria" id="CAI48522">
        <property type="protein sequence ID" value="CAI48522"/>
        <property type="gene ID" value="NP_0862A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700981"/>
    <dbReference type="KEGG" id="nph:NP_0862A"/>
    <dbReference type="eggNOG" id="arCOG02333">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_58_2"/>
    <dbReference type="OrthoDB" id="8127at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000156">
        <property type="term" value="F:phosphorelay response regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007234">
        <property type="term" value="P:osmosensory signaling via phosphorelay pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00075">
        <property type="entry name" value="HATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00156">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050351">
        <property type="entry name" value="BphY/WalK/GraS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42878:SF15">
        <property type="entry name" value="BACTERIOPHYTOCHROME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42878">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PAC"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Response regulatory" evidence="9">
        <location>
            <begin position="9"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="11">
        <location>
            <begin position="206"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="10">
        <location>
            <begin position="253"/>
            <end position="299"/>
        </location>
    </feature>
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        <location>
            <begin position="324"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="10">
        <location>
            <begin position="375"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="11">
        <location>
            <begin position="448"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="8">
        <location>
            <begin position="510"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="483"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI48522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="712" mass="78765" checksum="34D2EE364F6CD136" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUA0</accession>
    <name>A0A1U7EUA0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8 9">DNA repair and recombination protein RadA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 14" type="primary">radA</name>
        <name evidence="14" type="ordered locus">NP_0878A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 10">Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 10">Belongs to the eukaryotic RecA-like protein family.</text>
    </comment>
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        <property type="protein sequence ID" value="CAI48530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322166.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUA0"/>
    <dbReference type="STRING" id="348780.NP_0878A"/>
    <dbReference type="EnsemblBacteria" id="CAI48530">
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        <property type="gene ID" value="NP_0878A"/>
    </dbReference>
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    <dbReference type="KEGG" id="nph:NP_0878A"/>
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    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
    <dbReference type="GO" id="GO:0006310">
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    </dbReference>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Rad51_C"/>
    </dbReference>
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        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN DMC1_LIM15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942">
        <property type="entry name" value="RECA/RAD51/RADA DNA STRAND-PAIRING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8 10" id="KW-0227">DNA damage</keyword>
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    <keyword evidence="6 8 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecA family profile 1" evidence="12">
        <location>
            <begin position="80"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="RecA family profile 2" evidence="13">
        <location>
            <begin position="285"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="109"/>
            <end position="116"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="NCBIfam" id="TIGR02236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR005856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAI48530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="345" mass="37731" checksum="091AEFE0AC4D0C6E" modified="2017-05-10" version="1">MAATTADLEELPGVGPATADKLRENGYDSYQSIAVASPAELSNTADIGESNANDIIQAAREAADIGGFETGADVLDRREQIGKLEWLIPEIDEMLGGGVETQSITEVYGEFGAGKSQVTHQLAVNVQLPEEAGGLHGRCVFVDSEDTFRPERIDEMVRGLPEDVIEAALEEREIDGNPDDEETMEELVQSFLDKIHVAKAFNSNHQILLAEKAQEIAAEHEEGDYPVRLLCVDSLTAHFRAEYVGRGELADRQQKLNKHLHDIDRVGNLYNAATVVTNQVQSNPDAFFGDPTKPIGGNILGHKSTFRMYLKKSKGDKRIVKLVDAPNLPDGEAVMRVENDGLKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUA8</accession>
    <name>A0A1U7EUA8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">UPF0200 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0870A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322162.1">
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUA8"/>
    <dbReference type="STRING" id="348780.NP_0870A"/>
    <dbReference type="EnsemblBacteria" id="CAI48526">
        <property type="protein sequence ID" value="CAI48526"/>
        <property type="gene ID" value="NP_0870A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702617"/>
    <dbReference type="KEGG" id="nph:NP_0870A"/>
    <dbReference type="eggNOG" id="arCOG01045">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096329_0_0_2"/>
    <dbReference type="OrthoDB" id="85381at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41930:SF1">
        <property type="entry name" value="DEPHOSPHO-COA KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41930">
        <property type="entry name" value="UPF0200 PROTEIN MJ1399"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13207">
        <property type="entry name" value="AAA_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="177" mass="18975" checksum="644ECA506E811AD1" modified="2017-05-10" version="1">MRVIGTVGLAGSGKGEFAAVAEEQGVPVVTMGDVIREACRDRGLDPAEHHGEVAQSLREENGPAAIAEASLPHIESALEDAATVVVDGIRSGVEVDTFESAFGDDFTLVAIEAPFEERKDRINERGRDGDGETLAERDNRELRFGLDEALARANVRINNDGSLDAFRERAAEVLEST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUD0</accession>
    <name>A0A1U7EUD0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Iron-sulfur cluster assembly protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">iscU</name>
        <name evidence="2" type="ordered locus">NP_0940A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322197.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUD0"/>
    <dbReference type="STRING" id="348780.NP_0940A"/>
    <dbReference type="EnsemblBacteria" id="CAI48561">
        <property type="protein sequence ID" value="CAI48561"/>
        <property type="gene ID" value="NP_0940A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701005"/>
    <dbReference type="KEGG" id="nph:NP_0940A"/>
    <dbReference type="eggNOG" id="arCOG02077">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079283_4_0_2"/>
    <dbReference type="OrthoDB" id="319865at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06664">
        <property type="entry name" value="IscU_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002871">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01994">
        <property type="entry name" value="SUF_scaf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10093">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY ENZYME NIFU HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01592">
        <property type="entry name" value="NifU_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82649">
        <property type="entry name" value="SufE/NifU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NIF system FeS cluster assembly NifU N-terminal" evidence="1">
        <location>
            <begin position="7"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="138" mass="15211" checksum="BAA648FC32A981DA" modified="2017-05-10" version="1">MGIGSDMYRQQILDHYKNPRNYGELEDADIEHVGENPMCGDTIKMFLQLADDGDTIERVSFIGDGCAISQASASMLSEELRGESLEAVREMDRDDIIDMLGVDLSPMRIKCAVLAEKVAQDGIAIHQGDAEIDETTTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUD4</accession>
    <name>A0A1U7EUD4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Halocyanin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hcp3</name>
        <name evidence="4" type="ordered locus">NP_0938A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322196.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUD4"/>
    <dbReference type="STRING" id="348780.NP_0938A"/>
    <dbReference type="EnsemblBacteria" id="CAI48560">
        <property type="protein sequence ID" value="CAI48560"/>
        <property type="gene ID" value="NP_0938A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702802"/>
    <dbReference type="KEGG" id="nph:NP_0938A"/>
    <dbReference type="eggNOG" id="arCOG02921">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084115_1_2_2"/>
    <dbReference type="OrthoDB" id="11088at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04220">
        <property type="entry name" value="Halocyanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000923">
        <property type="entry name" value="BlueCu_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017533">
        <property type="entry name" value="Halocyanin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03102">
        <property type="entry name" value="halo_cynanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00127">
        <property type="entry name" value="Copper-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0186">Copper</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Blue (type 1) copper" evidence="3">
        <location>
            <begin position="65"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="153" mass="16069" checksum="68E6F4026884B15C" modified="2017-05-10" version="1">MDSTRRRMLGAIAGSLSVVAAGCLGGDDAGDAPDGVPQRIHEYVHTTQGYEESIPDRTGQSELRVAVGAGTGGLAFDPVVARIDAGTTVVWEWTGRGSTHNVVSDDGSDFSFESARHAEAGTTFEQPFDEPGIALYVCTPHRAQSMFGALDVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUJ4</accession>
    <name>A0A1U7EUJ4_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">tRNA (guanine(10)-N(2))-dimethyltransferase</fullName>
            <ecNumber evidence="13">2.1.1.213</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">tRNA:G10 dimethyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">trmG10</name>
        <name evidence="16" type="ordered locus">NP_1066A</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the adenosylmethionine-dependent methylation of the exocyclic amino group (N(2)) of guanosine at position 10 of various tRNAs. Acts via a two-step process that leads to the formation of either N(2)-monomethyl (m(2)G) or N(2)-dimethylguanosine (m(2)(2)G).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>guanosine(10) in tRNA + 2 S-adenosyl-L-methionine = N(2)-dimethylguanosine(10) in tRNA + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:43124"/>
            <dbReference type="Rhea" id="RHEA-COMP:10355"/>
            <dbReference type="Rhea" id="RHEA-COMP:10358"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74513"/>
            <dbReference type="EC" id="2.1.1.213"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the methyltransferase superfamily. Trm-G10 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.213" evidence="13"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322260.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUJ4"/>
    <dbReference type="STRING" id="348780.NP_1066A"/>
    <dbReference type="EnsemblBacteria" id="CAI48624">
        <property type="protein sequence ID" value="CAI48624"/>
        <property type="gene ID" value="NP_1066A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701045"/>
    <dbReference type="KEGG" id="nph:NP_1066A"/>
    <dbReference type="eggNOG" id="arCOG00047">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057819_1_0_2"/>
    <dbReference type="OrthoDB" id="7080at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160101">
        <property type="term" value="F:tRNA (guanine(10)-N2)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000251">
        <property type="entry name" value="Putative RNA methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000241">
        <property type="entry name" value="RlmKL-like_Mtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14911:SF21">
        <property type="entry name" value="N2-METHYLGUANOSINE TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14911">
        <property type="entry name" value="THUMP DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01170">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143437">
        <property type="entry name" value="THUMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 16" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="4" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Ribosomal RNA large subunit methyltransferase K/L-like methyltransferase" evidence="15">
        <location>
            <begin position="154"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00066936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00082665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAI48624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="321" mass="34009" checksum="1B7173E3576BF62A" modified="2017-05-10" version="1">MYLLELAGDDADPFAVCEARTRCSAVERLAPGLATARGVDTATVGTLAYTRRVCRLVGTCSPDIAAAAATLEAASVDRTGTVAVRARDVRGLADIDTQAAERRLGSVLVDRGFEVDLDAPDHTLVALFSADAAAVGWLSTETTRDFTDRRPTDKPFFQPGSMDPMDARAVANIAGAAPNVRILDPMCGTGGILVEAGLAGATPVGVDAQPKMVSGARQNLDHYLTDGAVLRGDATRLPFADDSFDGAVFDAPYGRQSKIEGQSLATLVGEALEEVRRVAGRTVLIADRPWNEAADAAGWTVAERFDRRVHGSLTRYIHVLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUJ6</accession>
    <name>A0A1U7EUJ6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Thioredoxin domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1100A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322277.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUJ6"/>
    <dbReference type="STRING" id="348780.NP_1100A"/>
    <dbReference type="EnsemblBacteria" id="CAI48641">
        <property type="protein sequence ID" value="CAI48641"/>
        <property type="gene ID" value="NP_1100A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702578"/>
    <dbReference type="KEGG" id="nph:NP_1100A"/>
    <dbReference type="eggNOG" id="arCOG04750">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126515_5_0_2"/>
    <dbReference type="OrthoDB" id="198458at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02980">
        <property type="entry name" value="TRX_Fd_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="109" mass="11861" checksum="2EC9F83EDF9431C7" modified="2017-05-10" version="1">MQRRTDEQTTRLDAYVFVCTNARDSEYAACADAGPGAEATVEAVTEWLRERDVYWNGVGVSTTDCLGLCSEGGTALTIQPHDEWFSDVTPEDVPELLAEAFGPDAAQLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUM4</accession>
    <name>A0A1U7EUM4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TRAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_1132A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322293.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUM4"/>
    <dbReference type="STRING" id="348780.NP_1132A"/>
    <dbReference type="EnsemblBacteria" id="CAI48657">
        <property type="protein sequence ID" value="CAI48657"/>
        <property type="gene ID" value="NP_1132A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701060"/>
    <dbReference type="KEGG" id="nph:NP_1132A"/>
    <dbReference type="eggNOG" id="arCOG01641">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121271_0_0_2"/>
    <dbReference type="OrthoDB" id="28569at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TRAM" evidence="2">
        <location>
            <begin position="71"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="53"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="130" mass="14040" checksum="463CBB807F09E865" modified="2017-05-10" version="1">MEISEKLLCLFSAEVEETEDKYVVEVPRREVDTGAIEAGDTYRVALISGAEAAQTETGGASAAPSDEPQPPVEEGEIRYVEIEDLGKQGDGIARVERGYVIIVPDAEVGERVKIEVSEVKSNFAVGEIIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUQ2</accession>
    <name>A0A1U7EUQ2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Ferredoxin (2Fe-2S)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">fdx</name>
        <name evidence="10" type="synonym">ferA1</name>
        <name evidence="10" type="ordered locus">NP_1272A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 2Fe2S plant-type ferredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322362.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUQ2"/>
    <dbReference type="STRING" id="348780.NP_1272A"/>
    <dbReference type="EnsemblBacteria" id="CAI48727">
        <property type="protein sequence ID" value="CAI48727"/>
        <property type="gene ID" value="NP_1272A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702701"/>
    <dbReference type="KEGG" id="nph:NP_1272A"/>
    <dbReference type="eggNOG" id="arCOG02844">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1773154_0_0_2"/>
    <dbReference type="OrthoDB" id="195646at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053441">
        <property type="entry name" value="2Fe2S_Ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041393">
        <property type="entry name" value="Frdxn_Halo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43112">
        <property type="entry name" value="FERREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43112:SF3">
        <property type="entry name" value="FERREDOXIN-2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="9">
        <location>
            <begin position="29"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI48727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="129" mass="14493" checksum="6CFEC7331CD1153E" modified="2017-05-10" version="1">MPTVEYLNYEVLDDHGWEMDDDDLFENAADADLDEEDYGSLDVAEGEYILEAAEAQGYDWPFSCRAGACANCAAIVKEGEIDMDMQQILSDEEVEEKMVRLTCIGSPAADEVKIVFNAKHLDYLQNRVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV11</accession>
    <name>A0A1U7EV11_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1482A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322467.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV11"/>
    <dbReference type="STRING" id="348780.NP_1482A"/>
    <dbReference type="EnsemblBacteria" id="CAI48832">
        <property type="protein sequence ID" value="CAI48832"/>
        <property type="gene ID" value="NP_1482A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701160"/>
    <dbReference type="KEGG" id="nph:NP_1482A"/>
    <dbReference type="eggNOG" id="ENOG502N5XG">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_893160_0_0_2"/>
    <dbReference type="OrthoDB" id="319029at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="311" mass="34006" checksum="FA2AADB7A238734A" modified="2017-05-10" version="1">MNRRRFLAAAGGGLAAGAATTLGVRLNNVRYYDPNGTVATDLPPGERIFAAARLLSVLDHRAVSRVTVLEDGTDSDPYRRSRYRYEWRPSRHRYLLVASTFAPVSGNPPPTRGGIYALLHHTEATDAAADETLPVATVAFASARRIIYDPLCDPPETVDARPQIDDERRRVMRGPGLGQSSDDRPPRPEAVLSAAATWTQTDETETTVTYETDSHEDYVAVPPLVTTATAVGDGSTIAATLDRETGRLLELDDRRVVEQETEDGRLREYTYRIETTFDQYGAVTAPEPDGPVPDASLSGTLEELLSDATLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV58</accession>
    <name>A0A1U7EV58_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">DUF4341/DUF395 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">NP_1570A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322510.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV58"/>
    <dbReference type="STRING" id="348780.NP_1570A"/>
    <dbReference type="EnsemblBacteria" id="CAI48876">
        <property type="protein sequence ID" value="CAI48876"/>
        <property type="gene ID" value="NP_1570A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701188"/>
    <dbReference type="KEGG" id="nph:NP_1570A"/>
    <dbReference type="eggNOG" id="arCOG04787">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122700_0_0_2"/>
    <dbReference type="OrthoDB" id="42259at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007272">
        <property type="entry name" value="Sulf_transp_TsuA/YedE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30574">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YEDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30574:SF1">
        <property type="entry name" value="SULPHUR TRANSPORT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04143">
        <property type="entry name" value="Sulf_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="22"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="74"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI48876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="175" mass="18067" checksum="364315F003A74831" modified="2017-05-10" version="1">MSTTALAVSAAMEFFPRGISHYLVGGLLIGLGTVVIYLGTGIIAGASTFLESTLSYVSKQDRFQQPRFVGSRDWRIVFTLSIVAGAAVYAIGFQGEAWSLSGSVWTTEVEAWRLFVGGLLVGVGTRLGKGCTSGHGICGVGSVSTTSILNVGIFMVIAIGTALAMVALGQGVSPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV61</accession>
    <name>A0A1U7EV61_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Copper-containing nitrite reductase</fullName>
            <ecNumber evidence="3">1.7.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">nirK</name>
        <name evidence="13" type="ordered locus">NP_1598A</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>nitric oxide + Fe(III)-[cytochrome c] + H2O = Fe(II)-[cytochrome c] + nitrite + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15233"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16301"/>
            <dbReference type="ChEBI" id="CHEBI:16480"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.7.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>Cu(+)</name>
            <dbReference type="ChEBI" id="CHEBI:49552"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotrimer.</text>
    </comment>
    <dbReference type="EC" id="1.7.2.1" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322524.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV61"/>
    <dbReference type="STRING" id="348780.NP_1598A"/>
    <dbReference type="EnsemblBacteria" id="CAI48890">
        <property type="protein sequence ID" value="CAI48890"/>
        <property type="gene ID" value="NP_1598A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702978"/>
    <dbReference type="KEGG" id="nph:NP_1598A"/>
    <dbReference type="eggNOG" id="arCOG03914">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031740_1_0_2"/>
    <dbReference type="OrthoDB" id="12293at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050421">
        <property type="term" value="F:nitrite reductase (NO-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd11020">
        <property type="entry name" value="CuRO_1_CuNIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04208">
        <property type="entry name" value="CuRO_2_CuNIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000093">
        <property type="entry name" value="Copper-containing nitrite reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001287">
        <property type="entry name" value="NO2-reductase_Cu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019546">
        <property type="entry name" value="TAT_signal_bac_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02376">
        <property type="entry name" value="Cu_nitrite_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01409">
        <property type="entry name" value="TAT_signal_seq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF394">
        <property type="entry name" value="FI03373P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00695">
        <property type="entry name" value="CUNO2RDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 10" id="KW-0186">Copper</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Plastocyanin-like" evidence="12">
        <location>
            <begin position="90"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="27"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="binding site" description="type 1 copper site" evidence="10">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="type 1 copper site" evidence="10">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="type 1 copper site" evidence="10">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="type 1 copper site" evidence="10">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="type 1 copper site" evidence="10">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="type 1 copper site" evidence="10">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="type 1 copper site" evidence="10">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601287-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAI48890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="362" mass="39040" checksum="50188DFE34F5A2DC" modified="2017-05-10" version="1">MTQNTNPNRRTFLKAAGAGGAFLLAGCVGNEGEPTDESTPEPAPQQAGLDPAQSVDTDRIAADPRDIPDPVDWDEPRHHEIEIATTEEIAEVEPGVTFQYMTFGGQVPGPMVRVREGDTINLTLTNESESAMPHNIDFHATYGPGGGADDTTIGPGESATIEFQAMYPGIHVYHCAVPNMDHHISAGMFGAILVEPEDGLPEVDHEFYFGQHELYTEGQAGEEGHHGFDFDSMRAEDPTYVCLNGEAYAFTGNGYGPMTVDKGDTVRVFFASGGPNLTSSVHPIGNVMSRYYRDGDLLSEPDRNIETAPVAPGTVAAWEMETPVPGPIKIVDHALSRVVRKGMMGVIEVEGELEPDIYNPDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV78</accession>
    <name>A0A1U7EV78_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Ribosome biogenesis protein Nop10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">nop10</name>
        <name evidence="9" type="ordered locus">NP_1620A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Involved in ribosome biogenesis; more specifically in 18S rRNA pseudouridylation and in cleavage of pre-rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NOP10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322535.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV78"/>
    <dbReference type="STRING" id="348780.NP_1620A"/>
    <dbReference type="EnsemblBacteria" id="CAI48901">
        <property type="protein sequence ID" value="CAI48901"/>
        <property type="gene ID" value="NP_1620A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701202"/>
    <dbReference type="KEGG" id="nph:NP_1620A"/>
    <dbReference type="eggNOG" id="arCOG00906">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_196480_1_0_2"/>
    <dbReference type="OrthoDB" id="7259at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.40">
        <property type="entry name" value="H/ACA ribonucleoprotein complex, subunit Nop10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00803">
        <property type="entry name" value="Nop10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007264">
        <property type="entry name" value="H/ACA_rnp_Nop10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036756">
        <property type="entry name" value="H/ACA_rnp_Nop10_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023532">
        <property type="entry name" value="Nop10_arc-typ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009623">
        <property type="entry name" value="PRK13130.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04135">
        <property type="entry name" value="Nop10p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144210">
        <property type="entry name" value="Nop10-like SnoRNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 7" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="32"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI48901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="60" mass="6827" checksum="A4E861ECF8EC33B4" modified="2017-05-10" version="1">MVKSDIRVCSAWETEHERPVYTLGDACPECGAPSKNSAPAPFSPDDPYGEYRRRARKRRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVC5</accession>
    <name>A0A1U7EVC5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transducer protein Htr22</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">htr22</name>
        <name evidence="9" type="ordered locus">NP_1756A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322602.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVC5"/>
    <dbReference type="STRING" id="348780.NP_1756A"/>
    <dbReference type="EnsemblBacteria" id="CAI48969">
        <property type="protein sequence ID" value="CAI48969"/>
        <property type="gene ID" value="NP_1756A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702837"/>
    <dbReference type="KEGG" id="nph:NP_1756A"/>
    <dbReference type="eggNOG" id="arCOG02320">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_18_2"/>
    <dbReference type="OrthoDB" id="8523at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06225">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.950">
        <property type="entry name" value="Methyl-accepting chemotaxis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004090">
        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32089:SF112">
        <property type="entry name" value="LYSOZYME-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32089">
        <property type="entry name" value="METHYL-ACCEPTING CHEMOTAXIS PROTEIN MCPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00260">
        <property type="entry name" value="CHEMTRNSDUCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158472">
        <property type="entry name" value="HAMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58104">
        <property type="entry name" value="Methyl-accepting chemotaxis protein (MCP) signaling domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0807">Transducer</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="64"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="8">
        <location>
            <begin position="87"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="8">
        <location>
            <begin position="175"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="7">
        <location>
            <begin position="239"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="520"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="131"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="205"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI48969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="543" mass="56615" checksum="F2F914071334FAD7" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVI3</accession>
    <name>A0A1U7EVI3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HTH domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1842A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322644.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVI3"/>
    <dbReference type="STRING" id="348780.NP_1842A"/>
    <dbReference type="EnsemblBacteria" id="CAI49012">
        <property type="protein sequence ID" value="CAI49012"/>
        <property type="gene ID" value="NP_1842A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701273"/>
    <dbReference type="KEGG" id="nph:NP_1842A"/>
    <dbReference type="eggNOG" id="arCOG06383">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1393616_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="199" mass="22241" checksum="9CF9B42AC026035B" modified="2017-05-10" version="1">MALPAWIEDRTEFDLNKKLTQRHVVETMLEAERPFFSAEQIRARVQPEVSKETVRNRLDELHEIDVVAAETYPESITLYYINHPESEWPLSPEGAEALSHSTPLETLSLADFVRLRNPAGIRTLVLAGFQLSLVLFVVGVVAAVAMVDPAVQSDNGFWAAAGNLFVLCLFLLLAERIARKVRTDGARSLLPTPEQFVSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVJ7</accession>
    <name>A0A1U7EVJ7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">3-hydroxyacyl-CoA dehydrogenase</fullName>
            <ecNumber evidence="6">1.1.1.35</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">hbd4</name>
        <name evidence="6" type="ordered locus">NP_1906A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.35" evidence="6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322676.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVJ7"/>
    <dbReference type="STRING" id="348780.NP_1906A"/>
    <dbReference type="EnsemblBacteria" id="CAI49044">
        <property type="protein sequence ID" value="CAI49044"/>
        <property type="gene ID" value="NP_1906A"/>
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    <dbReference type="GeneID" id="3702795"/>
    <dbReference type="KEGG" id="nph:NP_1906A"/>
    <dbReference type="eggNOG" id="arCOG00250">
        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_009834_2_0_2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="F:(3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Fatty oxidation complex, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022694">
        <property type="entry name" value="3-OHacyl-CoA_DH"/>
    </dbReference>
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        <property type="entry name" value="3-OHacyl-CoA_DH_NAD-bd"/>
    </dbReference>
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        <property type="entry name" value="3HC_DH_C"/>
    </dbReference>
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        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
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    </dbReference>
    <dbReference type="NCBIfam" id="NF005875">
        <property type="entry name" value="PRK07819.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48075">
        <property type="entry name" value="3-HYDROXYACYL-COA DEHYDROGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48075:SF5">
        <property type="entry name" value="3-HYDROXYBUTYRYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00725">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="3HCDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="1 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase NAD binding" evidence="5">
        <location>
            <begin position="10"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase C-terminal" evidence="4">
        <location>
            <begin position="189"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="14"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="2">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000105-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000105-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI49044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="285" mass="30629" checksum="62F64D0C7E83C406" modified="2017-05-10" version="1">MLTTDSLDRVGVVGAGTMGSGIAQVAAAAGYDVVMRDIEQEYVDSGFETIADSLDRKVKSGDYTEKEAEAIRDRIEGTTALGDLADCGLVIEAAVEDIDVKKDVFADLDGLVDDAVLATNTSTLSITTIASATTSPERVVGLHFMNPVPVMKGVEIVVGEKTRPGIVDFAHEFAAELGKETWESDDKPGFVTNRILMPWLNEGIRAFDEGVASKEDIDAGMKLGTNVPMGPLELADHIGLDVCLHASETLYEELGDRYKPAYLLKRKVEAGDLGKKTGEGFYDYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVK6</accession>
    <name>A0A1U7EVK6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Aminoglycoside phosphotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1926A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVK6"/>
    <dbReference type="EnsemblBacteria" id="CAI49054">
        <property type="protein sequence ID" value="CAI49054"/>
        <property type="gene ID" value="NP_1926A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_1926A"/>
    <dbReference type="HOGENOM" id="CLU_1901994_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="133" mass="15474" checksum="4C0D484289CFA77F" modified="2017-05-10" version="1">MLYHAYTDFSSVSLIDFDGVATGHPLEDVAIFKSALELIIQRLPYTGWTQYQKLCDAFDEGYLKTAPSETNSRKLEEFLHTIHHLTILLYYFERLPKKLESQCEYAKSSRLKLSALSIIDTWLLKRSLKRLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVP9</accession>
    <name>A0A1U7EVP9_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">fib</name>
        <name evidence="7" type="synonym">flpA</name>
        <name evidence="9" type="synonym">nop1:flpA</name>
        <name evidence="9" type="ordered locus">NP_2062A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in pre-rRNA and tRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in rRNA and tRNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with nop5. Component of box C/D small ribonucleoprotein (sRNP) particles that contain rpl7ae, FlpA and nop5, plus a guide RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the methyltransferase superfamily. Fibrillarin family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322751.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVP9"/>
    <dbReference type="STRING" id="348780.NP_2062A"/>
    <dbReference type="EnsemblBacteria" id="CAI49122">
        <property type="protein sequence ID" value="CAI49122"/>
        <property type="gene ID" value="NP_2062A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702721"/>
    <dbReference type="KEGG" id="nph:NP_2062A"/>
    <dbReference type="eggNOG" id="arCOG00078">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059055_2_0_2"/>
    <dbReference type="OrthoDB" id="6244at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990259">
        <property type="term" value="F:histone H2AQ104 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008649">
        <property type="term" value="F:rRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000494">
        <property type="term" value="P:box C/D sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00351">
        <property type="entry name" value="RNA_methyltransf_FlpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000692">
        <property type="entry name" value="Fibrillarin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020813">
        <property type="entry name" value="Fibrillarin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003276">
        <property type="entry name" value="PRK04266.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003278">
        <property type="entry name" value="PRK04266.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10335:SF17">
        <property type="entry name" value="FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10335">
        <property type="entry name" value="RRNA 2-O-METHYLTRANSFERASE FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01269">
        <property type="entry name" value="Fibrillarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006540">
        <property type="entry name" value="Nop17p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00052">
        <property type="entry name" value="FIBRILLARIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01206">
        <property type="entry name" value="Fibrillarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00566">
        <property type="entry name" value="FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 7" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="4 7 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 7" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="8"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="71"/>
            <end position="72"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="87"/>
            <end position="88"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="112"/>
            <end position="113"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="132"/>
            <end position="135"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI49122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="210" mass="22876" checksum="EF708C87330E827C" modified="2017-05-10" version="1">MSLPKGVQRRDFGDGERLATEGPPVYGEPTADGWRQWDPRRSKLGAMLETDIDTGLVGGETVLYLGAAAGTTVSHVADFAGPTYAVEFAARPARDLLTAAEPRPNLIPLLKDARKPDTYTHVVEPVDVLIQDVATRGQAKVALANRRFLRDDGRLLLAIKARSEDVTADPDAVFDDVLEELTTGYDIVETRSLERFHEDHLAVVAEPSGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVT0</accession>
    <name>A0A1U7EVT0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HEAT-PBS family taxis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2168A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322803.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVT0"/>
    <dbReference type="STRING" id="348780.NP_2168A"/>
    <dbReference type="EnsemblBacteria" id="CAI49175">
        <property type="protein sequence ID" value="CAI49175"/>
        <property type="gene ID" value="NP_2168A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701370"/>
    <dbReference type="KEGG" id="nph:NP_2168A"/>
    <dbReference type="eggNOG" id="arCOG02966">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053271_0_0_2"/>
    <dbReference type="OrthoDB" id="142930at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004155">
        <property type="entry name" value="PBS_lyase_HEAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12697:SF5">
        <property type="entry name" value="DEOXYHYPUSINE HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12697">
        <property type="entry name" value="PBS LYASE HEAT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13646">
        <property type="entry name" value="HEAT_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00567">
        <property type="entry name" value="EZ_HEAT"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="439" mass="47098" checksum="1198E27EB9221E6C" modified="2017-05-10" version="1">MSLYQLERERDLEDLVEVLQHSDNPSIRKRAAEVIGDILDTGDVEESDLVLESDETDDLGLEALEAEADRETVIDVLVRAVKDDENEGVRAAAVDALDKHSQEALERVVAELSGKDLDAAADWVAAKTFADVLEADQPELRMAAATGLGRVGDPSVTASLVDRLSDEDPRVRARSAVACGRIEDPRAVGPLEELITSDSNIDVRQAVARALGDIGTEGALSVLLDAAGDPSGSVRRVVADSLGQFGSVEPVDTLVGFLDDEHEMVRRTAMFSLVEILSNAPPQQSHEVRQAAADRLESATADEVIPPLTEILTESSGSPQRRNAAWLLGRVVGHQHREMAREALIEALGDDDGMTAQFAATSLTQLDDPGLERELLELARDETGDEDARSKALFVLGKVGGDRARDELGEFVDRTESDELREQAFSALSKLGGIGAGGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVX7</accession>
    <name>A0A1U7EVX7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2250A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322843.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVX7"/>
    <dbReference type="STRING" id="348780.NP_2250A"/>
    <dbReference type="EnsemblBacteria" id="CAI49216">
        <property type="protein sequence ID" value="CAI49216"/>
        <property type="gene ID" value="NP_2250A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703028"/>
    <dbReference type="KEGG" id="nph:NP_2250A"/>
    <dbReference type="eggNOG" id="arCOG00499">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044538_2_0_2"/>
    <dbReference type="OrthoDB" id="53037at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42663:SF12">
        <property type="entry name" value="ATP-BINDING PROTEIN PHNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42663">
        <property type="entry name" value="HYDROLASE C777.06C-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="35"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="274" mass="30069" checksum="D128A137CBFCA5C0" modified="2017-05-10" version="1">MEVTLLGTGDTTGTPTPSCKCETCQRARERGVERSRFSVHVHNDRTGESLLVDASPDFRSQFLSNDVDLPDAIVISHIHFDHLDGLGNAYRLLEDVPVFAADEVDPATGESVAETVGDKYDYLDAITVEPRSPFEPFETCGFEVTLVPVEHPPLVCYGLHIEDPETGATLALSGDSNYAIDDASRDVLRNPDLFFADGIVPAAYCEYHPAGGDHADADGTYRTFGTKHMTVEGARRLADDLDADDYRLVHLSHYIPADEAFDDDMAVDGERFEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW37</accession>
    <name>A0A1U7EW37_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Glutamine--fructose-6-phosphate aminotransferase [isomerizing]</fullName>
            <ecNumber evidence="2 9">2.6.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">D-fructose-6-phosphate amidotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">GFAT</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Glucosamine-6-phosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Hexosephosphate aminotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">L-glutamine--D-fructose-6-phosphate amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">glmS2</name>
        <name evidence="9" type="synonym">glmS</name>
        <name evidence="12" type="ordered locus">NP_2384A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>D-fructose 6-phosphate + L-glutamine = D-glucosamine 6-phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:13237"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="2.6.1.16"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.6.1.16" evidence="2 9"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322909.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW37"/>
    <dbReference type="STRING" id="348780.NP_2384A"/>
    <dbReference type="EnsemblBacteria" id="CAI49283">
        <property type="protein sequence ID" value="CAI49283"/>
        <property type="gene ID" value="NP_2384A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702753"/>
    <dbReference type="KEGG" id="nph:NP_2384A"/>
    <dbReference type="eggNOG" id="arCOG00057">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012520_7_1_2"/>
    <dbReference type="OrthoDB" id="372195at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004360">
        <property type="term" value="F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006047">
        <property type="term" value="P:UDP-N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00714">
        <property type="entry name" value="GFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05009">
        <property type="entry name" value="SIS_GlmS_GlmD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000006">
        <property type="entry name" value="Glutamine--fructose-6-phosphate aminotransferase [isomerizing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00164">
        <property type="entry name" value="GlmS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005855">
        <property type="entry name" value="GFAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047084">
        <property type="entry name" value="GFAT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035466">
        <property type="entry name" value="GlmS/AgaS_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035490">
        <property type="entry name" value="GlmS/FrlB_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01135">
        <property type="entry name" value="glmS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001484">
        <property type="entry name" value="PRK00331.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937">
        <property type="entry name" value="GLUCOSAMINE--FRUCTOSE-6-PHOSPHATE AMINOTRANSFERASE, ISOMERIZING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937:SF0">
        <property type="entry name" value="GLUTAMINE--FRUCTOSE-6-PHOSPHATE TRANSAMINASE (ISOMERIZING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13522">
        <property type="entry name" value="GATase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9 12" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 9 12" id="KW-0808">Transferase</keyword>
    <feature type="initiator methionine" description="Removed" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="10">
        <location>
            <begin position="2"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="11">
        <location>
            <begin position="279"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="11">
        <location>
            <begin position="443"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; for GATase activity" evidence="9">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="active site" description="For Fru-6P isomerization activity" evidence="9">
        <location>
            <position position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00055466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI49283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="594" mass="63339" checksum="C41351AA4C455617" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW58</accession>
    <name>A0A1U7EW58_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF420 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2436A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322935.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW58"/>
    <dbReference type="STRING" id="348780.NP_2436A"/>
    <dbReference type="EnsemblBacteria" id="CAI49309">
        <property type="protein sequence ID" value="CAI49309"/>
        <property type="gene ID" value="NP_2436A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701444"/>
    <dbReference type="KEGG" id="nph:NP_2436A"/>
    <dbReference type="eggNOG" id="arCOG02865">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104065_1_0_2"/>
    <dbReference type="OrthoDB" id="213478at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.210.10">
        <property type="entry name" value="Cytochrome c oxidase-like, subunit I domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036927">
        <property type="entry name" value="Cyt_c_oxase-like_su1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007352">
        <property type="entry name" value="DUF420"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37692:SF1">
        <property type="entry name" value="DUF420 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37692">
        <property type="entry name" value="HYPOTHETICAL MEMBRANE SPANNING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04238">
        <property type="entry name" value="DUF420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="200" mass="21370" checksum="D18DAD7010A2E5B1" modified="2017-05-10" version="1">MSTASAPSFARNHPKALATVVTLVGYVVVIGTLYGDFGLYPEISESTVDLLAHAIAVVNAATIACLVAGIYWIRADEIQKHRAAMLAAFGLILVFLVLYLLKTGGGGRKEILAGEPLRGAYLAMLGIHIFLSVVAVPFVLYAITLGLTRTPTELKSTAHATVGRIAVTTWLVSLVLGIVAYLMLTFYYGPEQVEFVRGMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW78</accession>
    <name>A0A1U7EW78_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Homolog to dipeptide epimerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">NP_2480A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mandelate racemase/muconate lactonizing enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322957.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW78"/>
    <dbReference type="STRING" id="348780.NP_2480A"/>
    <dbReference type="EnsemblBacteria" id="CAI49331">
        <property type="protein sequence ID" value="CAI49331"/>
        <property type="gene ID" value="NP_2480A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701458"/>
    <dbReference type="KEGG" id="nph:NP_2480A"/>
    <dbReference type="eggNOG" id="arCOG01168">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030273_4_3_2"/>
    <dbReference type="OrthoDB" id="372081at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016855">
        <property type="term" value="F:racemase and epimerase activity, acting on amino acids and derivatives"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009063">
        <property type="term" value="P:amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03319">
        <property type="entry name" value="L-Ala-DL-Glu_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034593">
        <property type="entry name" value="DgoD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034603">
        <property type="entry name" value="Dipeptide_epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029065">
        <property type="entry name" value="Enolase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018110">
        <property type="entry name" value="Mandel_Rmase/mucon_lact_enz_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013342">
        <property type="entry name" value="Mandelate_racemase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013341">
        <property type="entry name" value="Mandelate_racemase_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48080">
        <property type="entry name" value="D-GALACTONATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48080:SF3">
        <property type="entry name" value="ENOLASE SUPERFAMILY MEMBER DDB_G0284701"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13378">
        <property type="entry name" value="MR_MLE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02746">
        <property type="entry name" value="MR_MLE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00010">
        <property type="entry name" value="dipeptide_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00180">
        <property type="entry name" value="muconate_cycloisomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00009">
        <property type="entry name" value="o-succinylbenzoate_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00922">
        <property type="entry name" value="MR_MLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00908">
        <property type="entry name" value="MR_MLE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00909">
        <property type="entry name" value="MR_MLE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mandelate racemase/muconate lactonizing enzyme C-terminal" evidence="6">
        <location>
            <begin position="138"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="343" mass="35949" checksum="B3556C23C4A56D1F" modified="2017-05-10" version="1">MKASIERVELPLETPFTISRGTKRTVENLIVVIKHNGSTGYGAAAPARYYGETADTVAALLPELLDAVESIGDPHARNQVHERMATVAGDNPAAKAAVDTALWDLAGKLLGEPVYRLLGLSASASCPPTSYTVGLDDPETMAERAKAAVDAGYPVLKLKLGSERDRDIVRSVREIAPEATIRVDANEAWTPSAAVRNCKALADYGVEFVEQPVAAGNAEGLRRVYEQSPLPIAADESCVVAGDIPAVADSCDIVNLKLMKTGGLTPAVEAIYTARAHGLDVMCGCMVETNASIAAAAQLLPLLSYADLDGSLLLDSDPYEGVSVSDGRFDLGAVDCGTGVAPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW98</accession>
    <name>A0A1U7EW98_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Thioredoxin domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">NP_2536A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutaredoxin family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioredoxin family. DsbA subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322984.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW98"/>
    <dbReference type="STRING" id="348780.NP_2536A"/>
    <dbReference type="EnsemblBacteria" id="CAI49359">
        <property type="protein sequence ID" value="CAI49359"/>
        <property type="gene ID" value="NP_2536A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702550"/>
    <dbReference type="KEGG" id="nph:NP_2536A"/>
    <dbReference type="eggNOG" id="arCOG02868">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_47_1_2"/>
    <dbReference type="OrthoDB" id="15256at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13887:SF14">
        <property type="entry name" value="DISULFIDE BOND FORMATION PROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13887">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE KAPPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13462">
        <property type="entry name" value="Thioredoxin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Thioredoxin" evidence="9">
        <location>
            <begin position="15"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="163"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="163"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI49359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="209" mass="22101" checksum="685863577ED5BBF7" modified="2017-05-10" version="1">MSPDLTRRGYLAVTAAAGGAALAGCSDDVTADPDAIDDADRPALGAENAPVTVTVFEDYGCPACARFKAQALPALIDQYIETGEVRYLHADFPIPVDEAWSHPVANAAREVFFEAGNDAFWLFSSSIYDHQGSYSLDAIETVAADVADDEAVGAAARAAADEEAHSERIAADRDRGESWGVGGTPAVFVDETEVDPAYDEIDSAIQDRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWB8</accession>
    <name>A0A1U7EWB8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Dodecin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2576A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323004.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWB8"/>
    <dbReference type="STRING" id="348780.NP_2576A"/>
    <dbReference type="EnsemblBacteria" id="CAI49379">
        <property type="protein sequence ID" value="CAI49379"/>
        <property type="gene ID" value="NP_2576A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701494"/>
    <dbReference type="KEGG" id="nph:NP_2576A"/>
    <dbReference type="eggNOG" id="arCOG04561">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161196_1_1_2"/>
    <dbReference type="OrthoDB" id="187186at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1660.10">
        <property type="entry name" value="Flavin-binding protein dodecin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009923">
        <property type="entry name" value="Dodecin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025543">
        <property type="entry name" value="Dodecin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036694">
        <property type="entry name" value="Dodecin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041389">
        <property type="entry name" value="dodecin_Halo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39324">
        <property type="entry name" value="CALCIUM DODECIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39324:SF1">
        <property type="entry name" value="CALCIUM DODECIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07311">
        <property type="entry name" value="Dodecin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89807">
        <property type="entry name" value="Dodecin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="65" mass="7263" checksum="17E7F7587685BC44" modified="2017-05-10" version="1">MVFKKITLIGTSDESFDAAADDALDRAEDTLDNIYWAEVEEFGVEVANADGREYQAEVEVAFELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWG2</accession>
    <name>A0A1U7EWG2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Lrp/AsnC family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_2664A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323048.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWG2"/>
    <dbReference type="STRING" id="348780.NP_2664A"/>
    <dbReference type="EnsemblBacteria" id="CAI49423">
        <property type="protein sequence ID" value="CAI49423"/>
        <property type="gene ID" value="NP_2664A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701518"/>
    <dbReference type="KEGG" id="nph:NP_2664A"/>
    <dbReference type="eggNOG" id="arCOG01585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_4_0_2"/>
    <dbReference type="OrthoDB" id="33200at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050684">
        <property type="entry name" value="HTH-Siroheme_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011017">
        <property type="entry name" value="TRASH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056526">
        <property type="entry name" value="TRASH_HVO_1752"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413:SF4">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ASNC FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13404">
        <property type="entry name" value="HTH_AsnC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24273">
        <property type="entry name" value="TRASH_HVO_1752_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00746">
        <property type="entry name" value="TRASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="4"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="196" mass="21440" checksum="CF4DF725194AB216" modified="2017-05-10" version="1">MRDIDETDRELLRLLLEDGRRPYKELAEAVELSPPAVSDRIDRLRELGVIQRFTVDVDRSKLREGVRLAVTLSVRPGETTAVREALADVEGVEHVFATADSRLFVVATFPSGDIETHLAAAFDTGAVEAVEATPLVGADWHPALGEVTLGLECAECGNTVTDEGVSATIDGDRYEFCCGSCEARFEERYAELQQNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWM8</accession>
    <name>A0A1U7EWM8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2818A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323125.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWM8"/>
    <dbReference type="STRING" id="348780.NP_2818A"/>
    <dbReference type="EnsemblBacteria" id="CAI49500">
        <property type="protein sequence ID" value="CAI49500"/>
        <property type="gene ID" value="NP_2818A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701569"/>
    <dbReference type="KEGG" id="nph:NP_2818A"/>
    <dbReference type="HOGENOM" id="CLU_822873_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="337" mass="37562" checksum="1221B821FF22B2C1" modified="2017-05-10" version="1">MNRDELVANKTVGRRALLGSVAAFAGVVAGCSQLSPASSKENRPAENKTSKPSSSDIQDANTRLEYVSSEIESVPVVDENGELAFDTDAFEGSFDHERLFETVKDIRDSFEGQNTDRANELVPASRLLYSRIELRLLLNDVFASGTMYQHMFMRNNYGAASKHAEEALEALDYLPGNADTIEHRIDRLPGSQRVNGEYSTEVAKMEAAMAAEIYSWAVPAYRGLRDVSTGMKLVSESQTWEMGERSPDSPEHYTEISERFSSAKKEFNEAHGSGTPIPRMVPVFDDLRCFASDMDEVMADVATAAELSLEERHDESQRIREDALRRAEQNESRCLPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWN0</accession>
    <name>A0A1U7EWN0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Isopentenyl phosphate kinase</fullName>
            <shortName evidence="10">IPK</shortName>
            <ecNumber evidence="2 10">2.7.4.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">NP_2852A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the phosphorylation of isopentenyl phosphate (IP) to isopentenyl diphosphate (IPP). Functions in an alternate mevalonate (MVA) pathway leading to IPP, a key precursor for the biosynthesis of isoprenoid compounds such as archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>isopentenyl phosphate + ATP = isopentenyl diphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:33963"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:65078"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.26"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the isopentenyl phosphate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.26" evidence="2 10"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323142.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWN0"/>
    <dbReference type="STRING" id="348780.NP_2852A"/>
    <dbReference type="EnsemblBacteria" id="CAI49517">
        <property type="protein sequence ID" value="CAI49517"/>
        <property type="gene ID" value="NP_2852A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701572"/>
    <dbReference type="KEGG" id="nph:NP_2852A"/>
    <dbReference type="eggNOG" id="arCOG00860">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070213_0_0_2"/>
    <dbReference type="OrthoDB" id="15328at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102043">
        <property type="term" value="F:isopentenyl phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04241">
        <property type="entry name" value="AAK_FomA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024192">
        <property type="entry name" value="Fosfomycin_R_FomA-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040647">
        <property type="entry name" value="IPPK_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43654">
        <property type="entry name" value="GLUTAMATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43654:SF1">
        <property type="entry name" value="ISOPENTENYL PHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016496">
        <property type="entry name" value="Kin_FomA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="6 10 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aspartate/glutamate/uridylate kinase" evidence="13">
        <location>
            <begin position="1"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="6"/>
            <end position="10"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="12">
        <location>
            <position position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR016496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR016496-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR016496-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAI49517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="243" mass="24636" checksum="98241A37F7D09336" modified="2017-05-10" version="1">MTTVLKLGGSVVTEKATPETVDEEALQQAAAAIAAADVQPVIVHGGGSFGHHHAAAHGVSTTEGTDDAAAVRAITGAMRRLNDAVVGALADAGVPAVPLQPFAAGVREANDELRFCWEPVEAMLESGFVPVLCGDVVAHRGKGATILSGDELVVAAARAVSAERVGVCSSVPGVYDQTETVVDRIQSLEDIEEALGGSDVTDVTGGMAGKVKQLLALEAAGYIFDIDGLAAFLRGGNPGTRVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWN5</accession>
    <name>A0A1U7EWN5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type transport system ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">abc23a</name>
        <name evidence="7" type="ordered locus">NP_2802A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323117.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWN5"/>
    <dbReference type="STRING" id="348780.NP_2802A"/>
    <dbReference type="EnsemblBacteria" id="CAI49492">
        <property type="protein sequence ID" value="CAI49492"/>
        <property type="gene ID" value="NP_2802A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702436"/>
    <dbReference type="KEGG" id="nph:NP_2802A"/>
    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OrthoDB" id="87732at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03230">
        <property type="entry name" value="ABC_DR_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050763">
        <property type="entry name" value="ABC_transporter_ATP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711:SF5">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN NATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="2"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="288"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="288"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="298"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="311" mass="34470" checksum="6D7733B0F6562740" modified="2017-05-10" version="1">MIEVRDLRKEYGGFAAVEGSTFSVDSGEVFGVIGPNGAGKTTTLKAIAGLLEPTSGTVAINGEPADSRETRRRLGFLPEESPLYEEMSPRSYLRFFADLYDVPREVANQRIENTLDRLELAHRERALGDMSKGMKRKVAIARSLVNDPDVLVYDEPASGLDPLTTNYIIEFTKELAEEGRTVVFSAHNLYHVEEVCDRVAIMNEGKIVTKGPLSELRETYGETEYHVFTTVEVPGSVEDDDRYRRVVDDIDAVEAVREAAVERGGRLADIRTVEPSLEGLFLDVASGRNERNDRSAAEHSQQTTADRPTEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWT2</accession>
    <name>A0A1U7EWT2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">IS1341-type transposase ISNph26</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">NP_2942A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323186.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWT2"/>
    <dbReference type="STRING" id="348780.NP_2942A"/>
    <dbReference type="EnsemblBacteria" id="CAI49562">
        <property type="protein sequence ID" value="CAI49562"/>
        <property type="gene ID" value="NP_2942A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701605"/>
    <dbReference type="KEGG" id="nph:NP_2942A"/>
    <dbReference type="eggNOG" id="arCOG00683">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040997_0_0_2"/>
    <dbReference type="OrthoDB" id="295419at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="6">
        <location>
            <begin position="177"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="7">
        <location>
            <begin position="311"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="396"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="396"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="434" mass="49712" checksum="07D2335B7391C30F" modified="2017-05-10" version="1">MRRTNTFAVRPLSDDDERLLLDLLDASASLWNELNYERRQQFFDGESVWDTADYRKQYVGVLGSATAQQVIRKNKSAWQSFFAARENGEDTAPPGYWGNEDDGRELRTFIRHDLYTLDTGERSRVEIPVGQDLKDEYGLGYHDRLRLEVAGDPKWDGEQGRLEIQYGETDDTFRAFQPVTVPDSRQDSPLASHEAALDVGANNLVACTTTTGEQYLYEGRGLFAHFRETTREIARLQSKLREGRYSSQRIRRLYRKRTRRRDHAQDALVRDLMERLYAEGVDTVYVGDLTDVLSTHWCAEVNAKTHQFWAFRAFIERLSHTAEEYGITVEVRSEADTTRTCPACGEQDDTDRDGDVFRCPCGHEAHADLCASRTFLERQTGKPVGSMARPVRLKWDDHNWSEIPHSPERARPNEKRTNQSTDSQDGKLASVGTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWU3</accession>
    <name>A0A1U7EWU3_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Urease accessory protein UreH-like transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2970A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49576.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323200.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWU3"/>
    <dbReference type="STRING" id="348780.NP_2970A"/>
    <dbReference type="EnsemblBacteria" id="CAI49576">
        <property type="protein sequence ID" value="CAI49576"/>
        <property type="gene ID" value="NP_2970A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701611"/>
    <dbReference type="KEGG" id="nph:NP_2970A"/>
    <dbReference type="eggNOG" id="arCOG04638">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032635_1_0_2"/>
    <dbReference type="OrthoDB" id="162718at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039447">
        <property type="entry name" value="UreH-like_TM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42208:SF1">
        <property type="entry name" value="HEAVY METAL TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42208">
        <property type="entry name" value="HEAVY METAL TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13386">
        <property type="entry name" value="DsbD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="241"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Urease accessory protein UreH-like transmembrane" evidence="2">
        <location>
            <begin position="32"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49576.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="277" mass="28802" checksum="201D876D90785AAA" modified="2017-05-10" version="1">MQAVALPLASVLAQAHDHSTTLPTEHVDLLVFLVIGLLAGAHCLGMCGPLVTAYADRFNPQAGTRRANALTLYEVRQHALFNLGRAGSYALIGGLFGLIGALAFASTDAVTAVGDSVRGGVGLLVGIAIVASGLYYLRGQSGVPHGVPVIGTFFGWVSGLLTSRIDRLANSPGILGLGALHGLLPCPIIYPAYLYAFALGDPVRGALSLAVLGLGTIPTLFVYGTALGSLSTSSRVRLHRLLGVAFIVLGYIPIQHGLMLAFGIHLPHPPIPFYQPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWV6</accession>
    <name>A0A1U7EWV6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">FAD-dependent oxidoreductase (Homolog to geranylgeranyl reductase)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3024A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323226.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWV6"/>
    <dbReference type="STRING" id="348780.NP_3024A"/>
    <dbReference type="EnsemblBacteria" id="CAI49603">
        <property type="protein sequence ID" value="CAI49603"/>
        <property type="gene ID" value="NP_3024A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701630"/>
    <dbReference type="KEGG" id="nph:NP_3024A"/>
    <dbReference type="eggNOG" id="arCOG00570">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_590016_0_0_2"/>
    <dbReference type="OrthoDB" id="299107at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050407">
        <property type="entry name" value="Geranylgeranyl_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685:SF21">
        <property type="entry name" value="DEHYDROGENASE (FLAVOPROTEIN)-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685">
        <property type="entry name" value="GERANYLGERANYL DIPHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="1">
        <location>
            <begin position="4"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="451" mass="49416" checksum="31115ED0BA58DC59" modified="2017-05-10" version="1">MQQVDVAIVGGGPAGSAAGHAAATNGADAVVIEKGVPRADRDGLGPDSTDAAGILDYWVDIMDLGEPVPDHVKHRELNAAEFIGPEDRLELTDTSIDASYPNFGFTMHRARFDDWLRERAEAAGATYRVGEAVGDIESDLSGRPTHTVTLRDGTEFVADYLILADGPQRTVTDGVIGSWLPDSKTEGLETRRANHIAYQEYREFPAELFEDDRIKFWWGHMPGHTAYPWVFPNDGTVARVGLTMPIGLDIDDIDNRSAYPLLQPDDERLPQGGTYIERLLETVYPEYELADFPILEDRGKSNGTETYPISSTRPIESPTDANVAIVGGAMGATSAFHEGGDHVAVRTGKLAGELAATEALGSYNAAWHDAIGSEVRRNCVFADMVRGYEPDDWDHLFSLVDGLTSEDGFTPHEAIYAGWSGTKLFAEYKYRAFTFRNGGYAQVRESDYSIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWX1</accession>
    <name>A0A1U7EWX1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Probable secreted glycoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">NP_3026A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323227.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWX1"/>
    <dbReference type="STRING" id="348780.NP_3026A"/>
    <dbReference type="EnsemblBacteria" id="CAI49604">
        <property type="protein sequence ID" value="CAI49604"/>
        <property type="gene ID" value="NP_3026A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701631"/>
    <dbReference type="KEGG" id="nph:NP_3026A"/>
    <dbReference type="eggNOG" id="arCOG06150">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1207649_0_0_2"/>
    <dbReference type="OrthoDB" id="157640at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030115">
        <property type="term" value="C:S-layer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08547">
        <property type="entry name" value="Type_II_cohesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.680">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008965">
        <property type="entry name" value="CBM2/CBM3_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002102">
        <property type="entry name" value="Cohesin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026371">
        <property type="entry name" value="PGF_CTERM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04126">
        <property type="entry name" value="PGF_CTERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00963">
        <property type="entry name" value="Cohesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18204">
        <property type="entry name" value="PGF-CTERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49384">
        <property type="entry name" value="Carbohydrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Cohesin" evidence="4">
        <location>
            <begin position="35"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="PGF-CTERM archaeal protein-sorting signal" evidence="5">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="170"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="191"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF18204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI49604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="229" mass="23747" checksum="12E06CCF1B62262D" modified="2017-05-10" version="1">MRRLVAVVGCLAVAVAALAGGAAAADSPGLVYFEPDETEATAGETVDIDAEIRVISVYDNEAVESFEYTVEYDPELLSVDDIEEGPWLSGGNETTVAFETDIDEEAGAVTVSQARQPPAGGVIGEGTTATITFAVAADAPATNTTVAYTDASAQMLEYPLPVIETDASIEISDDGGTTNDEAEDGPAVTNDADDADDPAPEDQAGFGVVVAIAALFSVVLFGRRRYRML</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX30</accession>
    <name>A0A1U7EX30_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3174A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323300.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX30"/>
    <dbReference type="EnsemblBacteria" id="CAI49678">
        <property type="protein sequence ID" value="CAI49678"/>
        <property type="gene ID" value="NP_3174A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701676"/>
    <dbReference type="KEGG" id="nph:NP_3174A"/>
    <dbReference type="eggNOG" id="arCOG15026">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2257443_0_0_2"/>
    <dbReference type="OrthoDB" id="196475at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="104" mass="11497" checksum="5EB4D6B4412FB498" modified="2017-05-10" version="1">MIGAKRFGKKLAAGYGTDIDLGPEPSKAEIRDAVKQLKREEGLCGRRIGEQRLGPYFISGDDYGQRELDLLDEAIETEYAGSDLCIQTRRSECSRLRGGCKGNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX33</accession>
    <name>A0A1U7EX33_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate synthase</fullName>
            <shortName evidence="5">ADH synthase</shortName>
            <shortName evidence="5">ADHS</shortName>
            <shortName evidence="5">ADTH synthase</shortName>
            <ecNumber evidence="5 6">2.2.1.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">fba2</name>
        <name evidence="5" type="synonym">aroA'</name>
        <name evidence="8" type="ordered locus">NP_3160A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes a transaldol reaction between 6-deoxy-5-ketofructose 1-phosphate (DKFP) and L-aspartate semialdehyde (ASA) with an elimination of hydroxypyruvaldehyde phosphate to yield 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate (ADH). Plays a key role in an alternative pathway of the biosynthesis of 3-dehydroquinate (DHQ), which is involved in the canonical pathway for the biosynthesis of aromatic amino acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate + L-aspartate 4-semialdehyde = 2,3-dioxopropyl phosphate + 2-amino-2,3,7-trideoxy-D-lyxo-hept-6-ulosonate</text>
            <dbReference type="Rhea" id="RHEA:25952"/>
            <dbReference type="ChEBI" id="CHEBI:58859"/>
            <dbReference type="ChEBI" id="CHEBI:58860"/>
            <dbReference type="ChEBI" id="CHEBI:58861"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="2.2.1.10"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodecamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DeoC/FbaB aldolase family. ADHS subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.10" evidence="5 6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323293.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX33"/>
    <dbReference type="STRING" id="348780.NP_3160A"/>
    <dbReference type="EnsemblBacteria" id="CAI49671">
        <property type="protein sequence ID" value="CAI49671"/>
        <property type="gene ID" value="NP_3160A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702692"/>
    <dbReference type="KEGG" id="nph:NP_3160A"/>
    <dbReference type="eggNOG" id="arCOG04044">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057069_2_0_2"/>
    <dbReference type="OrthoDB" id="50091at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016744">
        <property type="term" value="F:transketolase or transaldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00958">
        <property type="entry name" value="DhnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00960">
        <property type="entry name" value="ADH_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010210">
        <property type="entry name" value="ADH_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002915">
        <property type="entry name" value="DeoC/FbaB/LacD_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050456">
        <property type="entry name" value="DeoC/FbaB_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041720">
        <property type="entry name" value="FbaB-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01949">
        <property type="entry name" value="ADH_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005556">
        <property type="entry name" value="PRK07226.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47916:SF1">
        <property type="entry name" value="3-HYDROXY-5-PHOSPHONOOXYPENTANE-2,4-DIONE THIOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47916">
        <property type="entry name" value="FRUCTOSE-BISPHOSPHATE ALDOLASE CLASS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01791">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038992">
        <property type="entry name" value="Aldolase_Ia"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01133">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 5" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0704">Schiff base</keyword>
    <keyword evidence="2 5 8" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="24"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5 7">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with dihydroxyacetone-P" evidence="7">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="5">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="24"/>
            <end position="28"/>
        </location>
        <ligand>
            <name>1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58861"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="143"/>
            <end position="145"/>
        </location>
        <ligand>
            <name>1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58861"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="198"/>
            <end position="199"/>
        </location>
        <ligand>
            <name>1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58861"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="225"/>
            <end position="226"/>
        </location>
        <ligand>
            <name>1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58861"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="NCBIfam" id="TIGR01949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR038992-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="262" mass="27186" checksum="28F0F5817259E5B2" modified="2017-05-10" version="1">MTPGIAARLDRVGTDGRYVVVPMDHGITLGAVNGLKDIESTIDAVTRGGADSVLTQKGIADRVHGNKNGAGYIVHLNGSTAIGPDSNDKRATGSVEAALRAGADAVSFHINVGSKYEREQIEALGRLTDQAAEYGLPVLAMTYARGPDIEGDDPEALGHAVRLGEELGADLIKTGYSGDAESFEHVCESTSLPVLIAGGSPDGDLESLRDVRGAMDAGAAGVSMGRTIFQHESPEHIARAVSLVVHDDYSAEAALEAAELSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX51</accession>
    <name>A0A1U7EX51_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable HNH-type endonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3256A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX51"/>
    <dbReference type="EnsemblBacteria" id="CAI49719">
        <property type="protein sequence ID" value="CAI49719"/>
        <property type="gene ID" value="NP_3256A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3256A"/>
    <dbReference type="eggNOG" id="arCOG03899">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1313165_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00085">
        <property type="entry name" value="HNHc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002711">
        <property type="entry name" value="HNH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003615">
        <property type="entry name" value="HNH_nuc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01844">
        <property type="entry name" value="HNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00507">
        <property type="entry name" value="HNHc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HNH nuclease" evidence="1">
        <location>
            <begin position="13"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="209" mass="23843" checksum="E8D3B3950AEB0447" modified="2017-05-10" version="1">MLIPNEKAKMCMEYRDRCLKTKGEYCHSCGVRQNIQVHHIDGDHSNNGLDNLVPLCANCHSKVHSGEIDWPPSRYRKLEVELSEENYDAVSGAAGSLGTEPEWLINAIIDRFRSEGGLQQVSTERLRSYESSEPETETNGIESEHAAAVYDILEPDEWMTIGELYEKYCTEVSNPKSKKTAKRYILRLCENGMATKRGRAKGRKYKRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX57</accession>
    <name>A0A1U7EX57_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CRISPR-associated endoribonuclease Cas2</fullName>
            <ecNumber evidence="8">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 9" type="primary">cas2</name>
        <name evidence="9" type="ordered locus">NP_3276A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Functions as a ssRNA-specific endoribonuclease. Involved in the integration of spacer DNA into the CRISPR cassette.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer, forms a heterotetramer with a Cas1 homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the CRISPR-associated endoribonuclease Cas2 protein family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="8"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX57"/>
    <dbReference type="STRING" id="348780.NP_3276A"/>
    <dbReference type="EnsemblBacteria" id="CAI49729">
        <property type="protein sequence ID" value="CAI49729"/>
        <property type="gene ID" value="NP_3276A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3276A"/>
    <dbReference type="eggNOG" id="arCOG04194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161124_3_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09725">
        <property type="entry name" value="Cas2_I_II_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01471">
        <property type="entry name" value="Cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021127">
        <property type="entry name" value="CRISPR_associated_Cas2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019199">
        <property type="entry name" value="Virulence_VapD/CRISPR_Cas2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01573">
        <property type="entry name" value="cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34405">
        <property type="entry name" value="CRISPR-ASSOCIATED ENDORIBONUCLEASE CAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34405:SF3">
        <property type="entry name" value="CRISPR-ASSOCIATED ENDORIBONUCLEASE CAS2 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09827">
        <property type="entry name" value="CRISPR_Cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143430">
        <property type="entry name" value="TTP0101/SSO1404-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="4 8 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5 8 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI49729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="92" mass="10567" checksum="76A222FDFD7CEF91" modified="2017-05-10" version="1">MTMRLAVAYDVSDDTNRRHVYRTLQRYGAWRQYSVFELEISKTDRVELEAELESHIDPSAGDRIRIYRLCSACQDATTDIGNDPPDGQSNVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX71</accession>
    <name>A0A1U7EX71_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">VTT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">NP_3350A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323386.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX71"/>
    <dbReference type="STRING" id="348780.NP_3350A"/>
    <dbReference type="DNASU" id="3701744"/>
    <dbReference type="EnsemblBacteria" id="CAI49766">
        <property type="protein sequence ID" value="CAI49766"/>
        <property type="gene ID" value="NP_3350A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701744"/>
    <dbReference type="KEGG" id="nph:NP_3350A"/>
    <dbReference type="eggNOG" id="arCOG04659">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104922_0_0_2"/>
    <dbReference type="OrthoDB" id="293407at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015414">
        <property type="entry name" value="TMEM64"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="VTT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12677">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP38-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12677:SF59">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP38-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="VTT_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="VTT" evidence="7">
        <location>
            <begin position="61"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="219" mass="22245" checksum="CDC7447F7CFBD854" modified="2017-05-10" version="1">MHRATVRQLFGAGVVAAIAAVAAISTSPQALFAAAERLVERPGLLAVTVVGLYLVRPLLAWPISALSILLGYLLGVAAAPIALAGAVVTTLPAYALARRLGHSEGVLARVGDAGAVVRRTTGDFRGIVAVRLAPLPTDPVSYAAGVAGVPLRPYVLGTAVGEAPWVLTAVLLGASTSQLTTAGAASNPLLVVTALALAALLALSRPAYRRLADDRPLRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX75</accession>
    <name>A0A1U7EX75_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3352A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323387.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX75"/>
    <dbReference type="EnsemblBacteria" id="CAI49767">
        <property type="protein sequence ID" value="CAI49767"/>
        <property type="gene ID" value="NP_3352A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701745"/>
    <dbReference type="KEGG" id="nph:NP_3352A"/>
    <dbReference type="eggNOG" id="arCOG04799">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117487_1_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043870">
        <property type="entry name" value="DUF5830"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19148">
        <property type="entry name" value="DUF5830"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="127" mass="13738" checksum="FCCBA4A280FC6BE4" modified="2017-05-10" version="1">MSRDASDDGDIEDPVELGVQLLARLEAPELPLPAVIDRIETITTHPETTRAILEAAERRGHISRDGETVTTAGGQFLSFESEVVTKEGDFECQRCGASISTGYFMQLEAGEHGPFGSSCIKKVTGRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX78</accession>
    <name>A0A1U7EX78_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CRISPR-associated protein Cas10d</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cas10d</name>
        <name evidence="2" type="synonym">csc3</name>
        <name evidence="2" type="ordered locus">NP_3264A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX78"/>
    <dbReference type="STRING" id="348780.NP_3264A"/>
    <dbReference type="EnsemblBacteria" id="CAI49723">
        <property type="protein sequence ID" value="CAI49723"/>
        <property type="gene ID" value="NP_3264A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3264A"/>
    <dbReference type="eggNOG" id="arCOG06925">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_288971_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09712">
        <property type="entry name" value="Cas10d_I-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017589">
        <property type="entry name" value="CRISPR-assoc_prot_Cas10d/Csc3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03174">
        <property type="entry name" value="cas_Csc3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="1023" mass="114135" checksum="BBDB24EBFA004089" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXA7</accession>
    <name>A0A1U7EXA7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">IS1341-type transposase ISNph12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">NP_3392A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323407.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXA7"/>
    <dbReference type="STRING" id="348780.NP_3392A"/>
    <dbReference type="EnsemblBacteria" id="CAI49787">
        <property type="protein sequence ID" value="CAI49787"/>
        <property type="gene ID" value="NP_3392A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701761"/>
    <dbReference type="KEGG" id="nph:NP_3392A"/>
    <dbReference type="eggNOG" id="arCOG00684">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_0_1_2"/>
    <dbReference type="OrthoDB" id="33505at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF11">
        <property type="entry name" value="RNA-GUIDED DNA ENDONUCLEASE RV2885C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="7">
        <location>
            <begin position="192"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="8">
        <location>
            <begin position="304"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="216"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI49787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="434" mass="49952" checksum="5FD38AA78758AD0D" modified="2017-05-10" version="1">MWYDYRFCAYPDRTGVAAEAERHIDIHRQAYNHTRYEYNALDTDEDNIGSAYQHQKRLTEWKDEWSVFSEVHSKALQKTVERFYDNLSNLSEKKQNGHKVGWLKWKPPREYQSVTYSQSGFELKNTSGRTATLWLSKIGDIPIRYHRPIPDNATIKEVTLKKETTGEWYVTFGLEVEDTTLPEKPDVDDLDAEDCVGIDLGITNYIYTSDGDSVDWLNLSDEYERLRREQRNLSRKEHRSNNWKKQRREVAKVKRRIKRKVEDFQHKLTTWLVKTYDAVFVEDLNVSGMLQQRGNARNKQDAAWRGFINMLEYKGDLYGTHVVQVNPAGTTKECAECGVETEKPLWVREHSCPSCGFEADRDANASYNVLSRGLRELGLGQAEVTPVETATAVETDGGRSSSVPASRVIETGSLGVVRKTESSVMTRDEVPRTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXB0</accession>
    <name>A0A1U7EXB0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Acyl-CoA synthetase</fullName>
            <ecNumber evidence="7">6.2.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">acs8</name>
        <name evidence="7" type="ordered locus">NP_3422A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.-" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323422.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXB0"/>
    <dbReference type="STRING" id="348780.NP_3422A"/>
    <dbReference type="EnsemblBacteria" id="CAI49802">
        <property type="protein sequence ID" value="CAI49802"/>
        <property type="gene ID" value="NP_3422A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702504"/>
    <dbReference type="KEGG" id="nph:NP_3422A"/>
    <dbReference type="eggNOG" id="arCOG00856">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_5_2"/>
    <dbReference type="OrthoDB" id="193284at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000008">
        <property type="entry name" value="2,3-dihydroxybenzoate-AMP ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004837">
        <property type="entry name" value="PRK06187.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43859">
        <property type="entry name" value="ACYL-ACTIVATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43859:SF4">
        <property type="entry name" value="BUTANOATE--COA LIGASE AAE1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="5">
        <location>
            <begin position="12"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="6">
        <location>
            <begin position="437"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="537" mass="60069" checksum="9FFFB7D15FEA7B94" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXC1</accession>
    <name>A0A1U7EXC1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Acyl-CoA dehydrogenase</fullName>
            <ecNumber evidence="10">1.3.8.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">acd3</name>
        <name evidence="10" type="ordered locus">NP_3460A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.-" evidence="10"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323441.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXC1"/>
    <dbReference type="STRING" id="348780.NP_3460A"/>
    <dbReference type="EnsemblBacteria" id="CAI49821">
        <property type="protein sequence ID" value="CAI49821"/>
        <property type="gene ID" value="NP_3460A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702478"/>
    <dbReference type="KEGG" id="nph:NP_3460A"/>
    <dbReference type="eggNOG" id="arCOG04350">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_0_2_2"/>
    <dbReference type="OrthoDB" id="275197at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00567">
        <property type="entry name" value="ACAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF20">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE FADE28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016578">
        <property type="entry name" value="HsaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0274">FAD</keyword>
    <keyword evidence="3 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 6 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="9">
        <location>
            <begin position="7"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="8">
        <location>
            <begin position="122"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="7">
        <location>
            <begin position="234"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI49821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="388" mass="42763" checksum="51628BB8B83D2D38" modified="2017-05-10" version="1">MATGESDLHDLIRESVRGIASEFDRGYWKEHIDQKEFPEEYWQALADDGWLGVTIPEEYGGEGLGMLEMCIIIEELSRSGGQGGIIFVLTPVFGGIGIQRHGNEAQKEEYLPQIADGEMKFCMALTEPTAGSNTLNIQTTADRDGDEFVVNGQKTFISGVENADTMLLVTRTTERDDENPTHGVTLFLVDDPAAQPGINLAEVDTAVPWFERQYQVTFDDLRVSEDDILGTEDGGLYLLWDTLNTERLAGAASSLGGGLRAIDLAVDYASDRSVFGQPIGAHQSIQHPIAESYAKLMSAREIMYKGAQKWDENEDCGLEANVAKLLTSQAGTEAADRAIQAHGGNGFTDEYEVYDIWQNLRLTQTVPVANELVLNYIAEHELDLPRSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXE5</accession>
    <name>A0A1U7EXE5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Phosphohexomutase (Phosphoglucomutase / phosphomannomutase)</fullName>
            <ecNumber evidence="12">5.4.2.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">pmm3</name>
        <name evidence="12" type="ordered locus">NP_3514A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.-" evidence="12"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323468.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXE5"/>
    <dbReference type="STRING" id="348780.NP_3514A"/>
    <dbReference type="EnsemblBacteria" id="CAI49848">
        <property type="protein sequence ID" value="CAI49848"/>
        <property type="gene ID" value="NP_3514A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703037"/>
    <dbReference type="KEGG" id="nph:NP_3514A"/>
    <dbReference type="eggNOG" id="arCOG00767">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016950_7_1_2"/>
    <dbReference type="OrthoDB" id="10363at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016868">
        <property type="term" value="F:intramolecular phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43771">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43771:SF1">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="Phosphoglucomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="9">
        <location>
            <begin position="2"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="10">
        <location>
            <begin position="143"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="11">
        <location>
            <begin position="248"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase C-terminal" evidence="8">
        <location>
            <begin position="365"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI49848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="448" mass="47292" checksum="90B2179B48B15484" modified="2017-05-10" version="1">MDLFGTAGIRGAAQTDVTPSLALAAGRAAAEDGATFVVGRDGRETGPALAAAVEAGLESGGADVYRLGAVPTPTLAYASRGRRGIMVTASHNPPADNGLKFFVDGTEYDRDAERRVESRVLSDPSPVEWDEWGDSYRMDVLEAYRTAVVEYVKTSFGDCEGLRVAVDCGNGMASHATPQVLRELGAEIVALNANVDGHFPGRESKPTAESLEALRTFVADGEFDLGIGHDGDADRIVVIDSSGDVVHEDTVLAILAKRYTAQASEPSPVVVTTPNASGRIDEAVEAAGGSVERVRLGALHEGIADASAGEGTVVFAAEPWKHTHTRFGPWIDGVASAAVLGALVADRGIEPLREPITERPYRKHNVDCPDEDKSGAMSLLESRLPEQFPEATATTDHGVRLAFDDGSWVLVRPSGTEPYLRLYAESESVDALVDDVAAVVRSAVAEAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXF3</accession>
    <name>A0A1U7EXF3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NUDIX family hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_3524A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323473.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXF3"/>
    <dbReference type="STRING" id="348780.NP_3524A"/>
    <dbReference type="EnsemblBacteria" id="CAI49853">
        <property type="protein sequence ID" value="CAI49853"/>
        <property type="gene ID" value="NP_3524A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701794"/>
    <dbReference type="KEGG" id="nph:NP_3524A"/>
    <dbReference type="eggNOG" id="arCOG01083">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1465051_0_0_2"/>
    <dbReference type="OrthoDB" id="346422at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43046:SF16">
        <property type="entry name" value="ADP-RIBOSE PYROPHOSPHATASE YJHB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43046">
        <property type="entry name" value="GDP-MANNOSE MANNOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="3">
        <location>
            <begin position="58"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="188" mass="20729" checksum="0DE79C8D0060190F" modified="2017-05-10" version="1">MTTVDGLWYLADEASQHAERAYHTLTEAHGEGTLQRTYERHVSRGRFRTLARRIQRTGTPYGAHTLVTRADGRVLLVRHEGVDLWVLPGGGVGPSETFSEAAERELHEEAGITADYDGLAMLNRVEVRCQGRQTWGVLPVFGAKASTVDLSVADPDGEISAARWFAPEQFPEDTRDRDDLVAAARAVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXF4</accession>
    <name>A0A1U7EXF4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Major facilitator superfamily transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">tp17</name>
        <name evidence="8" type="ordered locus">NP_3538A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323480.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXF4"/>
    <dbReference type="STRING" id="348780.NP_3538A"/>
    <dbReference type="EnsemblBacteria" id="CAI49860">
        <property type="protein sequence ID" value="CAI49860"/>
        <property type="gene ID" value="NP_3538A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703293"/>
    <dbReference type="KEGG" id="nph:NP_3538A"/>
    <dbReference type="eggNOG" id="arCOG00130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_5_14_2"/>
    <dbReference type="OrthoDB" id="204820at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050189">
        <property type="entry name" value="MFS_Efflux_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43124:SF3">
        <property type="entry name" value="CHLORAMPHENICOL EFFLUX PUMP RV0191"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43124">
        <property type="entry name" value="PURINE EFFLUX PUMP PBUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="87"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="214"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="286"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="312"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="348"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="376"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="22"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="401" mass="42018" checksum="0618A087E1356E42" modified="2017-05-10" version="1">MSIRSILRQEATALWGDGKGFALVAVAVGWGLLNGARMVYPVIIPYLQETYGLSLAVAGLLITVLWFFAAIGQLPGGILADRVDERVLMLAGTVVVAGALTLVVTSSSAAVLFFATAVWGLGHSVYPIARITFLSNLYRERVGSALGVTMATGDIGQTILPPVATVLAAGVAWQAGLGFIVPPLLVAGLFVVVTSSSRQPKPTRSESEPQRGMLAALKTLRTPAIGLMTGILFLYMFIWQSFTAFYPTYLTTVKGFAPATASLLFGFFFAVGVVVKPVAGTAYDRLGVRTSLVGVLLPATAGFLLLPLTDNVLFIVGITALISAMLGTGAITQSFLAEVFADDMKGAGLGVVRTLTAALAAGGPVVFGVIGEYGYFDWGYIALGVIMAVVVVLTLWMPKAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXG4</accession>
    <name>A0A1U7EXG4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3558A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXG4"/>
    <dbReference type="STRING" id="348780.NP_3558A"/>
    <dbReference type="EnsemblBacteria" id="CAI49870">
        <property type="protein sequence ID" value="CAI49870"/>
        <property type="gene ID" value="NP_3558A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3558A"/>
    <dbReference type="HOGENOM" id="CLU_3178675_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="46" mass="5453" checksum="2C3A5CDF20574100" modified="2017-05-10" version="1">MGLSDKRHIRADEKPSECENRREQREGLHDEDDCGDSDADRWRPIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXK6</accession>
    <name>A0A1U7EXK6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ferritin DpsA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">dpsA</name>
        <name evidence="4" type="ordered locus">NP_3658A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the Dps family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323538.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXK6"/>
    <dbReference type="STRING" id="348780.NP_3658A"/>
    <dbReference type="EnsemblBacteria" id="CAI49920">
        <property type="protein sequence ID" value="CAI49920"/>
        <property type="gene ID" value="NP_3658A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702670"/>
    <dbReference type="KEGG" id="nph:NP_3658A"/>
    <dbReference type="eggNOG" id="arCOG01101">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098183_0_1_2"/>
    <dbReference type="OrthoDB" id="8265at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01043">
        <property type="entry name" value="DPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054862">
        <property type="entry name" value="DNA_prot_starvation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002177">
        <property type="entry name" value="DPS_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008331">
        <property type="entry name" value="Ferritin_DPS_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041388">
        <property type="entry name" value="DNAstvprot_Halo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42932">
        <property type="entry name" value="GENERAL STRESS PROTEIN 20U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42932:SF1">
        <property type="entry name" value="GENERAL STRESS PROTEIN 20U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00210">
        <property type="entry name" value="Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005900">
        <property type="entry name" value="Dps"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01346">
        <property type="entry name" value="HELNAPAPROT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin/DPS" evidence="3">
        <location>
            <begin position="31"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="181" mass="20000" checksum="96CF0C853176CBEB" modified="2017-05-10" version="1">MSTQETVRRQAGSVEETALNIDPEKAEQLVDALNTDLAATYVLYHQIKKHHWNVEGAEFLDVHEYLGEVAADLEADADELAERAQAIGGVPLAGGATFEEHAPIDPEGDDVYDIRTSLENDIEAFGVIIDSLRDHIELANDLGDYTTEELLRDVLAGVEEHAHHLEHYLEDDTLVLDSATK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXL3</accession>
    <name>A0A1U7EXL3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3654A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323536.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXL3"/>
    <dbReference type="EnsemblBacteria" id="CAI49918">
        <property type="protein sequence ID" value="CAI49918"/>
        <property type="gene ID" value="NP_3654A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701835"/>
    <dbReference type="KEGG" id="nph:NP_3654A"/>
    <dbReference type="eggNOG" id="arCOG06243">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073527_1_0_2"/>
    <dbReference type="OrthoDB" id="241694at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055969">
        <property type="entry name" value="DUF7547"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24414">
        <property type="entry name" value="DUF7547"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="154"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="170"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="214"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="227" mass="23919" checksum="921CAD256C618814" modified="2017-05-10" version="1">MDDRERDLAEAADELAETLEALREELTEPRRGPLGIPQPPRPAELLRFTEQYTIPALISLFEASIRTLELLAAGIRLVDGRPVSTDDDRDRVVAAFEAAGREGGDRLAAASHTTLRKLDDALAELQAAAAGGDPGDEEIRELLSEARSLRAEVSDQLAAATDASDISSPDSKGGTTDAGTDTSDDDAGTDVSSSEVGIDVDEELASIKRTVDGGDSDADADATDNNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXS4</accession>
    <name>A0A1U7EXS4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HD family hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3860A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323638.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXS4"/>
    <dbReference type="STRING" id="348780.NP_3860A"/>
    <dbReference type="EnsemblBacteria" id="CAI50021">
        <property type="protein sequence ID" value="CAI50021"/>
        <property type="gene ID" value="NP_3860A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703099"/>
    <dbReference type="KEGG" id="nph:NP_3860A"/>
    <dbReference type="eggNOG" id="arCOG06870">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109398_2_0_2"/>
    <dbReference type="OrthoDB" id="147258at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008893">
        <property type="term" value="F:guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052194">
        <property type="entry name" value="MESH1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46246">
        <property type="entry name" value="GUANOSINE-3',5'-BIS(DIPHOSPHATE) 3'-PYROPHOSPHOHYDROLASE MESH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46246:SF1">
        <property type="entry name" value="GUANOSINE-3',5'-BIS(DIPHOSPHATE) 3'-PYROPHOSPHOHYDROLASE MESH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13328">
        <property type="entry name" value="HD_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="147" mass="16936" checksum="F15FEC36B8673334" modified="2017-05-10" version="1">MNDLEQAIDLAVDAYAGQTDKANETYIRHPLRVMDEMDTERERVVAVLHDVVEDANYTLEVIEQEFGPDIRNAVDALTKREDESYMEFVERAAANPTARKVKIADIEDNMDLTRLDSVNESVLEKQETYHEAWIKLQDMDQAEETTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXV7</accession>
    <name>A0A1U7EXV7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Homolog to cytochrome c-type biogenesis protein CcdA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3912A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323664.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXV7"/>
    <dbReference type="STRING" id="348780.NP_3912A"/>
    <dbReference type="EnsemblBacteria" id="CAI50047">
        <property type="protein sequence ID" value="CAI50047"/>
        <property type="gene ID" value="NP_3912A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701915"/>
    <dbReference type="KEGG" id="nph:NP_3912A"/>
    <dbReference type="eggNOG" id="arCOG04522">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1048085_0_0_2"/>
    <dbReference type="OrthoDB" id="205803at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="200"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="223" mass="22449" checksum="FB1A7D4161C618D6" modified="2017-05-10" version="1">MLDWALGGTVGWAFAAGVMTFFAPCSYPLLPGYVGFYVAQQETDAPLAGVAARGAAAGLGVFSVVAVISAAAVFVDGSQLEYISVFEPLVGVVFLLAGVAVLSGVSSPVSVPLPKRRASVLGFWVFGAGYGLAAVACLAPVFLSVLVQGLALPAPEATLVVGAYGGATAGLLTLATVAIAVGVDTWEDRTGWLMRHSKQIAGVLLVAAGIGQLLLAASDYGGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXW3</accession>
    <name>A0A1U7EXW3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC-type transport system ATP-binding protein (Probable substrate copper)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">nosF2</name>
        <name evidence="6" type="synonym">abc12a</name>
        <name evidence="6" type="ordered locus">NP_3924A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323670.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXW3"/>
    <dbReference type="STRING" id="348780.NP_3924A"/>
    <dbReference type="EnsemblBacteria" id="CAI50053">
        <property type="protein sequence ID" value="CAI50053"/>
        <property type="gene ID" value="NP_3924A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702405"/>
    <dbReference type="KEGG" id="nph:NP_3924A"/>
    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OrthoDB" id="40048at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03230">
        <property type="entry name" value="ABC_DR_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43335">
        <property type="entry name" value="ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43335:SF2">
        <property type="entry name" value="ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="8"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI50053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="244" mass="25163" checksum="C924BBE40B45AF4C" modified="2017-05-10" version="1">MTDETPILRISDLEHAYGSIDVLDGVSLDIDAGEVTAIIGPNGSGKTTLIRGIVGLHEPTSGTVEYTGPETTRPIGYLQQRPAFRPGETVEQTLSFYASLVGETDADVADRLEQVGLSAASDRDVDALSGGMTRLVGIAQATIGSPPVVVLDEPASGLDPGMSVRIFEVAAELADAGTAVLLTSHDLSLVERTADHVALLDAGEIAESGPPEAIRETVGADSLLSAFEDAITGDAGTVTVRGEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY21</accession>
    <name>A0A1U7EY21_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HTH domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4028A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323721.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY21"/>
    <dbReference type="STRING" id="348780.NP_4028A"/>
    <dbReference type="EnsemblBacteria" id="CAI50105">
        <property type="protein sequence ID" value="CAI50105"/>
        <property type="gene ID" value="NP_4028A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701946"/>
    <dbReference type="KEGG" id="nph:NP_4028A"/>
    <dbReference type="eggNOG" id="arCOG11371">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2930292_0_0_2"/>
    <dbReference type="OrthoDB" id="225412at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055741">
        <property type="entry name" value="DUF7317"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24001">
        <property type="entry name" value="DUF7317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="60" mass="6312" checksum="94387E600304815F" modified="2017-05-10" version="1">MNQHAMRTAMALYENGTLTAEAAARQAGVSVDRLKRAVRRAGGSTPSPTVDSGRPRVSAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY43</accession>
    <name>A0A1U7EY43_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bis(5'-nucleosyl)-tetraphosphatase [asymmetrical]</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">NP_4092A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323753.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY43"/>
    <dbReference type="STRING" id="348780.NP_4092A"/>
    <dbReference type="EnsemblBacteria" id="CAI50137">
        <property type="protein sequence ID" value="CAI50137"/>
        <property type="gene ID" value="NP_4092A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702510"/>
    <dbReference type="KEGG" id="nph:NP_4092A"/>
    <dbReference type="eggNOG" id="arCOG01078">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037162_14_4_2"/>
    <dbReference type="OrthoDB" id="25379at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004081">
        <property type="term" value="F:bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006167">
        <property type="term" value="P:AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006754">
        <property type="term" value="P:ATP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03428">
        <property type="entry name" value="NUDIX_Ap4A_Nudt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051325">
        <property type="entry name" value="Nudix_hydrolase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003565">
        <property type="entry name" value="Tetra_PHTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21340:SF0">
        <property type="entry name" value="BIS(5'-NUCLEOSYL)-TETRAPHOSPHATASE [ASYMMETRICAL]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21340">
        <property type="entry name" value="DIADENOSINE 5,5-P1,P4-TETRAPHOSPHATE PYROPHOSPHOHYDROLASE MUTT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="6">
        <location>
            <begin position="2"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI50137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="149" mass="17410" checksum="20632872E8F6AF9A" modified="2017-05-10" version="1">MIEATSAGAILFRDTRGRREYLLLKSRPGDWEFPKGGVEGNEELQQTAIREVKEEAGIEDFRLLDGFREDYDYVFEANGKTIHKTVHLFIARSFEASAELSEEHRDLQWRDYEQAINTITQDGPREILEEAHEFIDDYYEEDDEDESKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYC2</accession>
    <name>A0A1U7EYC2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MaoC domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">maoC1</name>
        <name evidence="2" type="ordered locus">NP_4248A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323831.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYC2"/>
    <dbReference type="STRING" id="348780.NP_4248A"/>
    <dbReference type="EnsemblBacteria" id="CAI50215">
        <property type="protein sequence ID" value="CAI50215"/>
        <property type="gene ID" value="NP_4248A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702686"/>
    <dbReference type="KEGG" id="nph:NP_4248A"/>
    <dbReference type="eggNOG" id="arCOG00774">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094876_0_1_2"/>
    <dbReference type="OrthoDB" id="296044at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd03451">
        <property type="entry name" value="FkbR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002539">
        <property type="entry name" value="MaoC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052342">
        <property type="entry name" value="MCH/BMMD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43664:SF1">
        <property type="entry name" value="BETA-METHYLMALYL-COA DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43664">
        <property type="entry name" value="MONOAMINE OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01575">
        <property type="entry name" value="MaoC_dehydratas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MaoC-like" evidence="1">
        <location>
            <begin position="11"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="152" mass="17207" checksum="345E62B4790A788C" modified="2017-05-10" version="1">MTGRYYEDFEVGETIEHDKRRTISEADNQQFCDMTMNQQPLHLDADFADDTEFGERLVNGLYTMSLAVGLSIPETTDGTIVANLSYDNVEHPNPVFHGDTIRVQSTVTDKRETSDGERGIVTMHVEVFKVNGKEEELVCSFDRTALSLKRPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYE8</accession>
    <name>A0A1U7EYE8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4292A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323853.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYE8"/>
    <dbReference type="STRING" id="348780.NP_4292A"/>
    <dbReference type="EnsemblBacteria" id="CAI50237">
        <property type="protein sequence ID" value="CAI50237"/>
        <property type="gene ID" value="NP_4292A"/>
    </dbReference>
    <dbReference type="GeneID" id="43500097"/>
    <dbReference type="KEGG" id="nph:NP_4292A"/>
    <dbReference type="eggNOG" id="arCOG11979">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_202934_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="49" mass="5525" checksum="C0A0F0A112E463D3" modified="2017-05-10" version="1">MSSDTSPEEIPVMQRLYNRIWLLAAVALLFFIVVYVGWGVADLLSVPSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYP9</accession>
    <name>A0A1U7EYP9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable amidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4566A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323989.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYP9"/>
    <dbReference type="STRING" id="348780.NP_4566A"/>
    <dbReference type="EnsemblBacteria" id="CAI50374">
        <property type="protein sequence ID" value="CAI50374"/>
        <property type="gene ID" value="NP_4566A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702100"/>
    <dbReference type="KEGG" id="nph:NP_4566A"/>
    <dbReference type="eggNOG" id="arCOG01717">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009600_0_4_2"/>
    <dbReference type="OrthoDB" id="359273at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895:SF7">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT A, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="1">
        <location>
            <begin position="24"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="477" mass="49786" checksum="B0653EBD743A33BE" modified="2017-05-10" version="1">MDICFDSAATIAAGVRNGEYEVADIVETTLDRIDERGAETNAYITVTASAARERAREVQAQVDEGAELPLAGVPVAIKDLSETKAGVPNTKGLEPLADTVAEETSVTVRRLEAAGAIVVGTTNTPELGHKVRTDNRLQGPTGTPFDTAYNAGGSSGGSAAALADGLCVLATGSDVGGSLRNPAACCGVVALKPSHGLVPRGNRVNGFRGHTPIGVLGPMARDVESLGMLLEVLAGKDSVDPLSVPQPDSYDEPDGSVVDSLRLAYSPDLDCFAVDPSVRKTVRSTLSSLEAAGATVEAVSLETPEYGELTHAYITAVTTFFATAVTELEASLGVDLLEAYDEEVPPELRTLVSMGRSHDINTYTGTEFPRTELFHAIEPVCKEYDALVCPSLATPPLTHDEPFPQEIDGQSTGGLPTDWTMAWPFNLTGHPVVNVPAGTVGGLPVGMQVVGETFSEATLLDIAAAVEAIEPWSYPGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYU7</accession>
    <name>A0A1U7EYU7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ISH14-type transposase ISNph1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4638A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324024.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYU7"/>
    <dbReference type="STRING" id="348780.NP_4638A"/>
    <dbReference type="EnsemblBacteria" id="CAI50410">
        <property type="protein sequence ID" value="CAI50410"/>
        <property type="gene ID" value="NP_4638A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702128"/>
    <dbReference type="KEGG" id="nph:NP_4638A"/>
    <dbReference type="eggNOG" id="arCOG02134">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107019_0_0_2"/>
    <dbReference type="OrthoDB" id="359563at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032874">
        <property type="entry name" value="DDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047930">
        <property type="entry name" value="Transpos_IS6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033587">
        <property type="entry name" value="transpos_IS6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39967">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39967:SF1">
        <property type="entry name" value="ISH14-TYPE TRANSPOSASE HSIRS44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13610">
        <property type="entry name" value="DDE_Tnp_IS240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE" evidence="1">
        <location>
            <begin position="82"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="211" mass="24343" checksum="6B8811631E09011F" modified="2017-05-10" version="1">MAEIARLSGRSDWIELDVVERERTPSELMKLGIRLHLAGLSLSNTIRELEKFGVNRSRKAVHDWVQKADLQPADDASPDHIALDETVIRINGQQFWLYAAVDPDTNELLHLRLFTTTTTALTQTFLRELQQKHDVEDAVFLVDYTQHLATALRRAGLRFQTVRHGNRNAVERVFRDIKRRTPSFSNSFSHVQPTTAEIWLQAFAVWWNSLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYU9</accession>
    <name>A0A1U7EYU9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Phosphohexomutase (Phosphoglucomutase / phosphomannomutase)</fullName>
            <ecNumber evidence="12">5.4.2.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">pmm2</name>
        <name evidence="12" type="ordered locus">NP_4676A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.-" evidence="12"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324043.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYU9"/>
    <dbReference type="STRING" id="348780.NP_4676A"/>
    <dbReference type="EnsemblBacteria" id="CAI50429">
        <property type="protein sequence ID" value="CAI50429"/>
        <property type="gene ID" value="NP_4676A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703036"/>
    <dbReference type="KEGG" id="nph:NP_4676A"/>
    <dbReference type="eggNOG" id="arCOG00767">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016950_7_1_2"/>
    <dbReference type="OrthoDB" id="10363at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008973">
        <property type="term" value="F:phosphopentomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05800">
        <property type="entry name" value="PGM_like2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45745:SF1">
        <property type="entry name" value="PHOSPHOGLUCOMUTASE 2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45745">
        <property type="entry name" value="PHOSPHOMANNOMUTASE 45A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="Phosphoglucomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="9">
        <location>
            <begin position="10"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="10">
        <location>
            <begin position="190"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="11">
        <location>
            <begin position="290"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase C-terminal" evidence="8">
        <location>
            <begin position="433"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI50429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="499" mass="52021" checksum="D18458648FCE6BB9" modified="2017-05-10" version="1">MDTANIETIAFGTDGWRDTREALTPIRLQAVAAAVSTVVAERGRREDPVAVGYDARAGASEAAREVAAGLAANGHDVLFAERDCPTPALAYAVVDRDLAGALTITASHNPPSDSGVKFIPEDGAPARPKQTDAIETALQSVDIPPLAPKPDIGPKPLATEAQITDGATESPVRDRPLSGHCEEVDFCRPHVDAVLERLGADLEGVTVAYDAMYGSGRGCTDAALAAAGADVIRLRCGRDPTFGGGAPNPTPGRLAALTDRVVDGPADIGIANDGDADRVAVVTEDGPLDANRLFAVLYDYLLAATDTPAPAVRTVSTTFLIDRIADAHGTTAVETPVGFKWVAETMAEHDALIGGEESGGFTIRDHIRLKDGVLTGVLAAMAAADQPLTDRLATLVDTYGDIKQARRSLECPDAKKAPTMAALTSHPPESITGTAVDEINDADGSKFLLADGAWLLVRPSGTEAKLRIYAEAATDDRVEALLTAGEALINDARESAELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ17</accession>
    <name>A0A1U7EZ17_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Hydroxymethylglutaryl-CoA synthase</fullName>
            <ecNumber evidence="7">2.3.3.10</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">hmgB</name>
        <name evidence="7" type="synonym">mvaB</name>
        <name evidence="7" type="ordered locus">NP_4836A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiolase-like superfamily. HMG-CoA synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.10" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324121.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ17"/>
    <dbReference type="STRING" id="348780.NP_4836A"/>
    <dbReference type="EnsemblBacteria" id="CAI50509">
        <property type="protein sequence ID" value="CAI50509"/>
        <property type="gene ID" value="NP_4836A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702958"/>
    <dbReference type="KEGG" id="nph:NP_4836A"/>
    <dbReference type="eggNOG" id="arCOG01767">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008065_3_2_2"/>
    <dbReference type="OrthoDB" id="5812at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004421">
        <property type="term" value="F:hydroxymethylglutaryl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006084">
        <property type="term" value="P:acetyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010142">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00827">
        <property type="entry name" value="init_cond_enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053544">
        <property type="entry name" value="HMG-CoA_Synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013746">
        <property type="entry name" value="HMG_CoA_synt_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013528">
        <property type="entry name" value="HMG_CoA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041302">
        <property type="entry name" value="HMG_CoAsyn_Halo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL COENZYME A SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323:SF2">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08540">
        <property type="entry name" value="HMG_CoA_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01154">
        <property type="entry name" value="HMG_CoA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hydroxymethylglutaryl-coenzyme A synthase N-terminal" evidence="5">
        <location>
            <begin position="3"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Hydroxymethylglutaryl-coenzyme A synthase C-terminal" evidence="6">
        <location>
            <begin position="195"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI50509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="445" mass="49618" checksum="47DA63A09F88806E" modified="2017-05-10" version="1">MTSVGIDAIEIHTGKLKLDLAETFAPAMDDAPEKYTKGLGLHASSFPDTYEDIVTMGANAAHRLMERKELEPDDIGRIDVATESAFDHSKPVSTYIAGCLEQVFEGDFHHANKGERKFACVAGTQSIDDAYNWIRAGRNRGRAAIVVATDTALYARDDPGESTQGAGAVAMLIDEDPDLVELSKAQGFGSADETDFLKPQQQFPSVDGKRSVNVYLARMREAMIDYQRVGGDIHPDDFRLGPFHTPFPGMVRKAAVLAYRHIIRDTDIEDALADDIGRQPRPEGFDDEEQYREALSEYTDALKETDRYQEWYDRTINPTLSISRQVGNWYTGSVHVARVSGLKALAEAGEDAVGDQLLVGSYGSGAQAEIHVETIQDGWEAELDALNIDEQLDSRYDLDFEEYEDVHDAHNYDEETAVEEFTAPDSEFVFDGWGRMGERQYRYVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ32</accession>
    <name>A0A1U7EZ32_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Archaeal Type IV pilin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4846A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324126.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ32"/>
    <dbReference type="STRING" id="348780.NP_4846A"/>
    <dbReference type="EnsemblBacteria" id="CAI50514">
        <property type="protein sequence ID" value="CAI50514"/>
        <property type="gene ID" value="NP_4846A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702194"/>
    <dbReference type="KEGG" id="nph:NP_4846A"/>
    <dbReference type="eggNOG" id="arCOG02425">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_516417_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012859">
        <property type="entry name" value="Pilin_N_archaeal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07790">
        <property type="entry name" value="Pilin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Archaeal Type IV pilin N-terminal" evidence="1">
        <location>
            <begin position="10"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="527" mass="56043" checksum="DB472152FF8F7883" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ67</accession>
    <name>A0A1U7EZ67_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4948A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324177.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ67"/>
    <dbReference type="STRING" id="348780.NP_4948A"/>
    <dbReference type="EnsemblBacteria" id="CAI50565">
        <property type="protein sequence ID" value="CAI50565"/>
        <property type="gene ID" value="NP_4948A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702208"/>
    <dbReference type="KEGG" id="nph:NP_4948A"/>
    <dbReference type="HOGENOM" id="CLU_1870801_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="136" mass="14046" checksum="3E92022642223B8E" modified="2017-05-10" version="1">MAAPGVERRTFTDGPTASTADGPAAHYPLVGDDQPASSGDAGAGAPFADTTGETGVRLPYGFVEADEASALRPPAIEVSGGAEAVLEWCGDSSCSTRGTDSGCRRGVSFDHVFDEEVLSLCYLRADRQRGHAVIVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZH9</accession>
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        <recommendedName>
            <fullName evidence="1">DUF8054 domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="2" type="ordered locus">NP_5242A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZH9"/>
    <dbReference type="STRING" id="348780.NP_5242A"/>
    <dbReference type="EnsemblBacteria" id="CAI50712">
        <property type="protein sequence ID" value="CAI50712"/>
        <property type="gene ID" value="NP_5242A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702309"/>
    <dbReference type="KEGG" id="nph:NP_5242A"/>
    <dbReference type="eggNOG" id="arCOG06294">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098906_0_0_2"/>
    <dbReference type="OrthoDB" id="267121at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058367">
        <property type="entry name" value="DUF8054"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26239">
        <property type="entry name" value="DUF8054"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8054" evidence="1">
        <location>
            <begin position="2"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="176" mass="18782" checksum="75AE17B8271B431F" modified="2017-05-10" version="1">MIPDGTLCRSRVVTDLGAELDAICRRRLDGYIVVAPQRTLLGDDGDRSIITFEDGIARLAYHPESDRGGPAALGRLGPGPYRVDRYELDAAADLDIPHGAVSLQVPPGGVAERLADDPAVADRIREAAAEQAAGDDSDPVAAFLENEAAIDDIRETARAEAHERAAEWGLEDALED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZJ3</accession>
    <name>A0A1U7EZJ3_NATPD</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5248A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324325.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZJ3"/>
    <dbReference type="EnsemblBacteria" id="CAI50715">
        <property type="protein sequence ID" value="CAI50715"/>
        <property type="gene ID" value="NP_5248A"/>
    </dbReference>
    <dbReference type="GeneID" id="54763333"/>
    <dbReference type="KEGG" id="nph:NP_5248A"/>
    <dbReference type="eggNOG" id="arCOG15186">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_204972_2_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055976">
        <property type="entry name" value="DUF7554"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24431">
        <property type="entry name" value="DUF7554"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="56" mass="6004" checksum="C4E6BEC4F2041EF2" modified="2017-05-10" version="1">MNDLDVETLLKIVLVLVGILLVLEVVGRFISILASLGPLVAALIAVLIVAYLLDYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZL2</accession>
    <name>A0A1U7EZL2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5352A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZL2"/>
    <dbReference type="STRING" id="348780.NP_5352A"/>
    <dbReference type="EnsemblBacteria" id="CAI50767">
        <property type="protein sequence ID" value="CAI50767"/>
        <property type="gene ID" value="NP_5352A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702346"/>
    <dbReference type="KEGG" id="nph:NP_5352A"/>
    <dbReference type="HOGENOM" id="CLU_1399779_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="198" mass="20573" checksum="C2F51B0D9EE6AC4D" modified="2017-05-10" version="1">MTRTTLILTLTVVLATTFAGAVAAPALAEETDDGTDDYVELTFDEVDDEIVASAVDTNTDHDVTNVDDELPDIDLSGLEIGTTVTFEWDAVDADELQIEDEDGDVLETDDGDELTDDTGTLEADWNDSLDDDADAYHFDNAALYDDDEDAVGTIGGSFFGGGGVGDIGDDIDNTSLGIGAVGALVLVVGGIALAGRAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZP8</accession>
    <name>A0A1U7EZP8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2491G</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CCI69580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939532.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZP8"/>
    <dbReference type="STRING" id="348780.NP_2491G"/>
    <dbReference type="EnsemblBacteria" id="CCI69580">
        <property type="protein sequence ID" value="CCI69580"/>
        <property type="gene ID" value="NP_2491G"/>
    </dbReference>
    <dbReference type="GeneID" id="25139687"/>
    <dbReference type="KEGG" id="nph:NP_2491G"/>
    <dbReference type="eggNOG" id="arCOG06372">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109222_0_0_2"/>
    <dbReference type="OrthoDB" id="242095at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCI69580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="172" mass="17393" checksum="861C2341046C0944" modified="2017-05-10" version="1">MSRDRTTTRDRSGDVSEESLEFGTGDALGDAGGAETPETTDADRTDAGLTGRAKSKATSLFSPRSFLIAFALIAVGLFATSTFLPLPGAGLVGVFAAAFLFGFVVNERRYAEVAAAGGGAVAASTFLDFAVVAVLGGFGISLTILGGLGGAAAGLLGAYFGRDLRDGLTREI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZQ6</accession>
    <name>A0A1U7EZQ6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Small CPxCG-related zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2331E</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CCI69578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZQ6"/>
    <dbReference type="STRING" id="348780.NP_2331E"/>
    <dbReference type="EnsemblBacteria" id="CCI69578">
        <property type="protein sequence ID" value="CCI69578"/>
        <property type="gene ID" value="NP_2331E"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_2331E"/>
    <dbReference type="HOGENOM" id="CLU_2784214_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCI69578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="68" mass="7405" checksum="55CE7F3B7115A36A" modified="2017-05-10" version="1">MASEQRGDGDIEQSTDGTERCSECGSRAVFLTYESREDGRCAHCRLGIPWVNSSVDTDTDRSGGSDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILX3</accession>
    <name>Q3ILX3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_6234A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324494.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILX3"/>
    <dbReference type="EnsemblBacteria" id="CAI50897">
        <property type="protein sequence ID" value="CAI50897"/>
        <property type="gene ID" value="NP_6234A"/>
    </dbReference>
    <dbReference type="GeneID" id="32155036"/>
    <dbReference type="KEGG" id="nph:NP_6234A"/>
    <dbReference type="HOGENOM" id="CLU_1860760_0_0_2"/>
    <dbReference type="OrthoDB" id="381228at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="137" mass="15146" checksum="BFB4F2B648055EDD" modified="2005-11-08" version="1">MQRRKFSTTTGSALSILFAGCLGGTDVPDNENGGENAVEERIKTCEEQYIQDEVVTRADETIDDTLQPAVVDSESRGDGEFVELRTEFGVTRQADDAPDEHIDYLVTAYYLVSDDIVYRTEGDEPEGDPRDGITIDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILY6</accession>
    <name>Q3ILY6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Thermosome subunit 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ths4</name>
        <name evidence="7" type="ordered locus">NP_6206A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7 8">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324481.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILY6"/>
    <dbReference type="EnsemblBacteria" id="CAI50883">
        <property type="protein sequence ID" value="CAI50883"/>
        <property type="gene ID" value="NP_6206A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694602"/>
    <dbReference type="KEGG" id="nph:NP_6206A"/>
    <dbReference type="HOGENOM" id="CLU_008891_7_3_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="514"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI50883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="548" mass="57891" checksum="F5FB2A5825CBB52B" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILZ8</accession>
    <name>Q3ILZ8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Family 3 CoA transferase</fullName>
            <ecNumber evidence="2">2.8.3.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_6182A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.8.3.-" evidence="2"/>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324469.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILZ8"/>
    <dbReference type="EnsemblBacteria" id="CAI50871">
        <property type="protein sequence ID" value="CAI50871"/>
        <property type="gene ID" value="NP_6182A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694638"/>
    <dbReference type="KEGG" id="nph:NP_6182A"/>
    <dbReference type="HOGENOM" id="CLU_033975_0_0_2"/>
    <dbReference type="OrthoDB" id="28444at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008410">
        <property type="term" value="F:CoA-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10540">
        <property type="entry name" value="Crotonobetainyl-coa:carnitine coa-transferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.10">
        <property type="entry name" value="formyl-coa transferase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050483">
        <property type="entry name" value="CoA-transferase_III_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003673">
        <property type="entry name" value="CoA-Trfase_fam_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044855">
        <property type="entry name" value="CoA-Trfase_III_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023606">
        <property type="entry name" value="CoA-Trfase_III_dom_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48207">
        <property type="entry name" value="SUCCINATE--HYDROXYMETHYLGLUTARATE COA-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48207:SF3">
        <property type="entry name" value="SUCCINATE--HYDROXYMETHYLGLUTARATE COA-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02515">
        <property type="entry name" value="CoA_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89796">
        <property type="entry name" value="CoA-transferase family III (CaiB/BaiF)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="404" mass="44335" checksum="3CD2B5D87E5AABA6" modified="2005-11-08" version="1">MAGEARNPNGDTGPLDGLTVLDASRVLVGPFCTMQLGDLGADVIKIERPDGGDQTRAWHPPTYGDSEESAYYTSINRNKRSVALNLQSDEGQAAFRELAAEADVLVENFRVGKMEEWGLGYETLAEENPELIFCSLSGYGEWGPDKDRPAYDIMMQAEGGLMSITGEENGPPVRVGVAIADIGAGMYATQAILAALLHRELGDGTGQKIDVSLLDGQVAWMTYMASYYFATGDPPERMGSKHPTIAPYQAFETADGYVVIASGSEHIWPRFCIAIDREELIDDERFATNAARVEHRDELDPLLEEELRQYSTEEVLARMDEAGVPASKVRDMEDVFEHPQVQARGMHKTVSHPTIGDVEMPGSPMHFSETPTSIEKHPPLLGEHTEEVFEEYGLPEAGIPDDID</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM30</accession>
    <name>Q3IM30_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">TrkA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">trkA2</name>
        <name evidence="9" type="synonym">tp30u1</name>
        <name evidence="9" type="ordered locus">NP_6110A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9 10">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a potassium transport system.</text>
    </comment>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324437.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM30"/>
    <dbReference type="EnsemblBacteria" id="CAI50835">
        <property type="protein sequence ID" value="CAI50835"/>
        <property type="gene ID" value="NP_6110A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694606"/>
    <dbReference type="KEGG" id="nph:NP_6110A"/>
    <dbReference type="HOGENOM" id="CLU_046525_2_3_2"/>
    <dbReference type="OrthoDB" id="24929at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015079">
        <property type="term" value="F:potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="entry name" value="Regulator of K+ conductance, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006036">
        <property type="entry name" value="K_uptake_TrkA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050721">
        <property type="entry name" value="Trk_Ktr_HKT_K-transport"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43833">
        <property type="entry name" value="POTASSIUM CHANNEL PROTEIN 2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43833:SF5">
        <property type="entry name" value="TRK SYSTEM POTASSIUM UPTAKE PROTEIN TRKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00335">
        <property type="entry name" value="KUPTAKETRKA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="TrkA C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0630">Potassium</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RCK N-terminal" evidence="7">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="8">
        <location>
            <begin position="133"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI50835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="225" mass="23988" checksum="E831151B1DB5F676" modified="2005-11-08" version="1">MYLIIVGAGKIGSNLIEMATGDGNDVVVIEEDEAVADRISATYDCLVLCADATESDILEEAGIDRAEAVISTTNLDAVNTMVMLLAREHDVPSLVSVVHDPAHIPIFEQIGVNIVENPQNLIADHLYHSVRYPGVQDFMSLGENSEFIEIEPKAGAPVTEHPLSEVHDRGILPDDALVAAIKRGDDVLTPRGETTVAVGDLVTVLVNDTAVEAVLSAFGHDSNGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM45</accession>
    <name>Q3IM45_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DoxX domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">NP_6076A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6 7">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324422.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM45"/>
    <dbReference type="EnsemblBacteria" id="CAI50818">
        <property type="protein sequence ID" value="CAI50818"/>
        <property type="gene ID" value="NP_6076A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694703"/>
    <dbReference type="KEGG" id="nph:NP_6076A"/>
    <dbReference type="HOGENOM" id="CLU_124808_0_0_2"/>
    <dbReference type="OrthoDB" id="199518at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032808">
        <property type="entry name" value="DoxX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39157:SF1">
        <property type="entry name" value="DOXX FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39157">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07681">
        <property type="entry name" value="DoxX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="96"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="137"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI50818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="178" mass="19964" checksum="B6EE0F8807B2DEB1" modified="2005-11-08" version="1">MATQNKRTTTKLFGRGVSYGTSGQWNSYWLLFLRLVMGWWFFSSGLSKIVEYGVLYDAHGWMMFGTEGTIVYPITAWFAANAVWLPNLLIPWGQLLIGLGLIVGCLTRLAAANGAVLMFFFYFGNAEWGNGFVNGDLFGLLLFLTIIVFAAGRVWGIDAYLEGTNYVKNRPKLRYILG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-09-11" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMN2</accession>
    <name>KAE1B_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="1">tRNA N6-adenosine threonylcarbamoyltransferase</fullName>
                <ecNumber evidence="1">2.3.1.234</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName>N6-L-threonylcarbamoyladenine synthase</fullName>
                <shortName>t(6)A synthase</shortName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="1">t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="1">tRNA threonylcarbamoyladenosine biosynthesis protein Kae1</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="1">Serine/threonine-protein kinase Bud32</fullName>
                <ecNumber evidence="1">2.7.11.1</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name type="ordered locus">NP_5070A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-threonylcarbamoyladenylate + adenosine(37) in tRNA = N(6)-L-threonylcarbamoyladenosine(37) in tRNA + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:37059"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.3.1.234"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="1">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the KAE1 / TsaD family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.234" evidence="1"/>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324236.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMN2"/>
    <dbReference type="SMR" id="Q3IMN2"/>
    <dbReference type="STRING" id="348780.NP_5070A"/>
    <dbReference type="EnsemblBacteria" id="CAI50626">
        <property type="protein sequence ID" value="CAI50626"/>
        <property type="gene ID" value="NP_5070A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702246"/>
    <dbReference type="KEGG" id="nph:NP_5070A"/>
    <dbReference type="eggNOG" id="arCOG01185">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023208_2_2_2"/>
    <dbReference type="OrthoDB" id="6818at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000408">
        <property type="term" value="C:EKC/KEOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061711">
        <property type="term" value="F:tRNA N(6)-L-threonylcarbamoyladenine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01446">
        <property type="entry name" value="Kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01447">
        <property type="entry name" value="Kae1_Bud32_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022495">
        <property type="entry name" value="Bud32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017861">
        <property type="entry name" value="KAE1/TsaD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034680">
        <property type="entry name" value="Kae1_archaea_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009220">
        <property type="entry name" value="tRNA_threonyl_synthase/kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03724">
        <property type="entry name" value="arch_bud32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03722">
        <property type="entry name" value="arch_KAE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00329">
        <property type="entry name" value="gcp_kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007174">
        <property type="entry name" value="PRK09605.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735:SF14">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036401">
        <property type="entry name" value="Gcp_STYKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00789">
        <property type="entry name" value="OSIALOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000303659" description="Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein">
        <location>
            <begin position="1"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="338"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Kae1">
        <location>
            <begin position="1"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for kinase activity" evidence="1">
        <location>
            <position position="452"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="134"/>
            <end position="138"/>
        </location>
        <ligand>
            <name>L-threonylcarbamoyladenylate</name>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>L-threonylcarbamoyladenylate</name>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>L-threonylcarbamoyladenylate</name>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>L-threonylcarbamoyladenylate</name>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>L-threonylcarbamoyladenylate</name>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="345"/>
            <end position="352"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01447"/>
        </source>
    </evidence>
    <sequence length="533" mass="57263" checksum="03E030815ECABE24" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IN28</accession>
    <name>GCST_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable aminomethyltransferase</fullName>
            <ecNumber evidence="1">2.1.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glycine cleavage system T protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gcvT</name>
        <name type="ordered locus">NP_4774A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The glycine cleavage system catalyzes the degradation of glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N(6)-[(R)-S(8)-aminomethyldihydrolipoyl]-L-lysyl-[protein] + (6S)-5,6,7,8-tetrahydrofolate = N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16945"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:10492"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="2.1.2.10"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GcvT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.10" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324090.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IN28"/>
    <dbReference type="SMR" id="Q3IN28"/>
    <dbReference type="STRING" id="348780.NP_4774A"/>
    <dbReference type="EnsemblBacteria" id="CAI50478">
        <property type="protein sequence ID" value="CAI50478"/>
        <property type="gene ID" value="NP_4774A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702746"/>
    <dbReference type="KEGG" id="nph:NP_4774A"/>
    <dbReference type="eggNOG" id="arCOG00756">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007884_10_2_2"/>
    <dbReference type="OrthoDB" id="2001at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004047">
        <property type="term" value="F:aminomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.110:FF:000003">
        <property type="entry name" value="Aminomethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="entry name" value="Probable tRNA modification gtpase trme, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00259">
        <property type="entry name" value="GcvT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006223">
        <property type="entry name" value="GcvT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028896">
        <property type="entry name" value="GcvT/YgfZ/DmdA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029043">
        <property type="entry name" value="GcvT/YgfZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022903">
        <property type="entry name" value="GcvT_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013977">
        <property type="entry name" value="GcvT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006222">
        <property type="entry name" value="GCVT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00528">
        <property type="entry name" value="gcvT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001567">
        <property type="entry name" value="PRK00389.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43757">
        <property type="entry name" value="AMINOMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43757:SF2">
        <property type="entry name" value="AMINOMETHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01571">
        <property type="entry name" value="GCV_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08669">
        <property type="entry name" value="GCV_T_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006487">
        <property type="entry name" value="GcvT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101790">
        <property type="entry name" value="Aminomethyltransferase beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103025">
        <property type="entry name" value="Folate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0032">Aminotransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000047681" description="Probable aminomethyltransferase">
        <location>
            <begin position="1"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00259"/>
        </source>
    </evidence>
    <sequence length="358" mass="38159" checksum="0BB8413B93E05142" modified="2005-11-08" version="1">MSLRTPPLFDAHTDAGAKLTEFGGWEMPVEFDSIRTEHDAVRESAGKFDISHMGEIEVSGPDAAALTNRLTTNDVASLAPGEATYAGITDDDGVLLDDTVVYRLPDGATYLFVPNAGNDELMAERWRSFADRWDLAATVDNATDDYAMVALQGPDALELLSSLDVDVFDLSRFEAAERTVAGVDCLVSRTGYTGEDGVELLFETAAAETVWSALDCQPCGLGARDTLRLEAGFLLGGNEFDPETNPRTPYEANIGFAVDLGTEFVGSDALAAQREAGPDERLVGLRLQDRGIARHGHDIVAAGDVVGEVTSGTMSPTLGAAIALGYVPVEYTEPGTELAVVVRGDEKDAVVEALPFYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q3INS6</accession>
    <name>Q3INS6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Succinate dehydrogenase subunit C</fullName>
            <ecNumber evidence="9">1.3.5.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">sdhC</name>
        <name evidence="9" type="ordered locus">NP_4270A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="9"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323842.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3INS6"/>
    <dbReference type="SMR" id="Q3INS6"/>
    <dbReference type="STRING" id="348780.NP_4270A"/>
    <dbReference type="EnsemblBacteria" id="CAI50226">
        <property type="protein sequence ID" value="CAI50226"/>
        <property type="gene ID" value="NP_4270A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703197"/>
    <dbReference type="KEGG" id="nph:NP_4270A"/>
    <dbReference type="eggNOG" id="arCOG02244">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127125_1_0_2"/>
    <dbReference type="OrthoDB" id="21304at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.10">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase, transmembrane subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039023">
        <property type="entry name" value="SdhC_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034804">
        <property type="entry name" value="SQR/QFR_C/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014314">
        <property type="entry name" value="Succ_DH_cytb556"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000701">
        <property type="entry name" value="SuccDH_FuR_B_TM-su"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02970">
        <property type="entry name" value="succ_dehyd_cytB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41910">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE 2 MEMBRANE SUBUNIT SDHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41910:SF1">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE HYDROPHOBIC MEMBRANE ANCHOR SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01127">
        <property type="entry name" value="Sdh_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81343">
        <property type="entry name" value="Fumarate reductase respiratory complex transmembrane subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="31"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI50226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="130" mass="14309" checksum="42361990C9B978B5" modified="2005-11-08" version="1">MSQSYDRGLVEDFGRWQEFSAGMWAWIFHKFTGWVLIGYLFTHVAVLSTATVDAATYTQTLQGLESLLVVRFLEVGLLAVAVFHILNGIRLLFVDLGVGLEAQDKAFYAALIVTAAITVASIPTFLMGAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q3INS7</accession>
    <name>Q3INS7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Succinate dehydrogenase subunit D</fullName>
            <ecNumber evidence="9">1.3.5.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">sdhD</name>
        <name evidence="9" type="ordered locus">NP_4268A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="9"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323841.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3INS7"/>
    <dbReference type="STRING" id="348780.NP_4268A"/>
    <dbReference type="EnsemblBacteria" id="CAI50225">
        <property type="protein sequence ID" value="CAI50225"/>
        <property type="gene ID" value="NP_4268A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703198"/>
    <dbReference type="KEGG" id="nph:NP_4268A"/>
    <dbReference type="eggNOG" id="arCOG04162">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2032801_0_0_2"/>
    <dbReference type="OrthoDB" id="187639at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd03500">
        <property type="entry name" value="SQR_TypeA_SdhD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.10">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase, transmembrane subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034804">
        <property type="entry name" value="SQR/QFR_C/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000701">
        <property type="entry name" value="SuccDH_FuR_B_TM-su"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01127">
        <property type="entry name" value="Sdh_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81343">
        <property type="entry name" value="Fumarate reductase respiratory complex transmembrane subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="59"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI50225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="121" mass="13312" checksum="F79230717E81F147" modified="2005-11-08" version="1">MAEHYSSFEPKGTRWLLQRITAVFLVAVLAYHFMLLHFVNHAAEITFAGTQARMSEVGYFITMVLFLIAGAFHGVNGVYNALVNQGLKGTQKTVVKWLLIVAGALLIVQGIRVAIAMTGLF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IP82</accession>
    <name>AMPPA_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AMP phosphorylase</fullName>
            <shortName evidence="1">AMPpase</shortName>
            <ecNumber evidence="1">2.4.2.57</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Nucleoside monophosphate phosphorylase</fullName>
            <shortName evidence="1">NMP phosphorylase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">deoA</name>
        <name type="ordered locus">NP_3958A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of AMP and phosphate to adenine and ribose 1,5-bisphosphate (R15P). Exhibits phosphorylase activity toward CMP and UMP in addition to AMP. Functions in an archaeal AMP degradation pathway, together with R15P isomerase and RubisCO.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + phosphate = alpha-D-ribose 1,5-bisphosphate + adenine</text>
            <dbReference type="Rhea" id="RHEA:36975"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:68688"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.4.2.57"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>CMP + phosphate = cytosine + alpha-D-ribose 1,5-bisphosphate</text>
            <dbReference type="Rhea" id="RHEA:36987"/>
            <dbReference type="ChEBI" id="CHEBI:16040"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:68688"/>
            <dbReference type="EC" id="2.4.2.57"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>UMP + phosphate = alpha-D-ribose 1,5-bisphosphate + uracil</text>
            <dbReference type="Rhea" id="RHEA:36991"/>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:68688"/>
            <dbReference type="EC" id="2.4.2.57"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.57" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323686.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IP82"/>
    <dbReference type="SMR" id="Q3IP82"/>
    <dbReference type="STRING" id="348780.NP_3958A"/>
    <dbReference type="EnsemblBacteria" id="CAI50070">
        <property type="protein sequence ID" value="CAI50070"/>
        <property type="gene ID" value="NP_3958A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702662"/>
    <dbReference type="KEGG" id="nph:NP_3958A"/>
    <dbReference type="eggNOG" id="arCOG02013">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025040_6_0_2"/>
    <dbReference type="OrthoDB" id="9827at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004645">
        <property type="term" value="F:1,4-alpha-oligoglucan phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006196">
        <property type="term" value="P:AMP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046125">
        <property type="term" value="P:pyrimidine deoxyribonucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006206">
        <property type="term" value="P:pyrimidine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.970.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1030.10">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.30">
        <property type="entry name" value="Pyrimidine nucleoside phosphorylase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02132">
        <property type="entry name" value="AMP_phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017713">
        <property type="entry name" value="AMP_phosphorylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000312">
        <property type="entry name" value="Glycosyl_Trfase_fam3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017459">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036320">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035902">
        <property type="entry name" value="Nuc_phospho_transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036566">
        <property type="entry name" value="PYNP-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013102">
        <property type="entry name" value="PYNP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017872">
        <property type="entry name" value="Pyrmidine_PPase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013466">
        <property type="entry name" value="Thymidine/AMP_Pase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000053">
        <property type="entry name" value="Thymidine/pyrmidine_PPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03327">
        <property type="entry name" value="AMP_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02645">
        <property type="entry name" value="ARCH_P_rylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003338">
        <property type="entry name" value="PRK04350.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10515">
        <property type="entry name" value="THYMIDINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10515:SF0">
        <property type="entry name" value="THYMIDINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02885">
        <property type="entry name" value="Glycos_trans_3N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00591">
        <property type="entry name" value="Glycos_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07831">
        <property type="entry name" value="PYNP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00941">
        <property type="entry name" value="PYNP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52418">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47648">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54680">
        <property type="entry name" value="Pyrimidine nucleoside phosphorylase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00647">
        <property type="entry name" value="THYMID_PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000314731" description="AMP phosphorylase">
        <location>
            <begin position="1"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="254"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="192"/>
            <end position="197"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02132"/>
        </source>
    </evidence>
    <sequence length="500" mass="52699" checksum="7E1341E905A7E241" modified="2005-11-08" version="1">MELTVEPIDIGTERPVVLLNCADAETLGVHSLDRVEIDWDGTTEVGIVKVTDELVAAGRIGASHGFPEITDGTVVAVTPAGQPESVESIRRKLDGRELDSDELGAIVADIEADRLSDLELSAYVCASHANGLSLEETKQLTERMAEVGKQLSWEQPVVADKHSIGGVAGNRVTPVVVAIVAAAGLTIPKTSSRAVTSPAGTADTMEVFCPVEFSREEIRDIVTETGGCLVWGGAVDLSPVDDKVIRAQRPLSLDPPGQVIASVLSKKQSAGSSHIVVDIPYGAGAKVTSLSEARDLADDFRRVGDHLGLTIECALTRGSDPIGHGIGPVLEARDVLAVLEGEGPEPLRIKSLRLADIIFDMAREAGMPVDDRSAADILDSGAALSKFRDIVAVQGGDPDVSRDDLQPGDRTETVTADTDGLVVDVDNQAVSQLARRAGAPNDHGAGVVIHRRTGDKAVAGDVLYTIHAESSDRLEAAREYAAGDEIVRVGGRDEALVERR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IPN4</accession>
    <name>Q3IPN4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acetyl-CoA C-acetyltransferase catalytic subunit</fullName>
            <ecNumber evidence="4">2.3.1.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">acaB7</name>
        <name evidence="4" type="ordered locus">NP_3650A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.9" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323534.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IPN4"/>
    <dbReference type="STRING" id="348780.NP_3650A"/>
    <dbReference type="EnsemblBacteria" id="CAI49916">
        <property type="protein sequence ID" value="CAI49916"/>
        <property type="gene ID" value="NP_3650A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702465"/>
    <dbReference type="KEGG" id="nph:NP_3650A"/>
    <dbReference type="eggNOG" id="arCOG01278">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035425_4_0_2"/>
    <dbReference type="OrthoDB" id="167534at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003985">
        <property type="term" value="F:acetyl-CoA C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00829">
        <property type="entry name" value="SCP-x_thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055140">
        <property type="entry name" value="Thiolase_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870">
        <property type="entry name" value="ACETYL-COA C-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870:SF1">
        <property type="entry name" value="NON-SPECIFIC LIPID-TRANSFER PROTEIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22691">
        <property type="entry name" value="Thiolase_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="1" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="2">
        <location>
            <begin position="4"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="3">
        <location>
            <begin position="238"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="384" mass="40632" checksum="DBCE0F5DDC7701E3" modified="2005-11-08" version="1">MPDVAVIGASMTNFGTREKWIRELLAEAGKDCLDDAGVTPGEVDHLYVSNMASGEFEGQTGIQNALAHDLSVVPAYAQRVGQTSSSGAAGIYAAWQSIASGASEMTLLVGGEKMTHRTTGESSDIIASITHPVEYKHGVTLPSFAGLAARLYLDEYDAPRESLAEVAVKNHHNGVKNPDAQFKKKIDVETALESPLVADPLRLYDYCPVTDGSAALLFCPVDQAREYADEFAVVSGVGGATDTHVVHERPDPTSMSAVVESGRQAYEMAGVEPEDIDVAELHDMVTILELLQMEAFGFSEEGAAWKDVKAGRTELDGELPINPSGGLKARGHPLGGTGVAQAYELYTQLVGDAGDRQVDADIGLACNIGGFGNCVNTTIMEASQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IRB3</accession>
    <name>KTHY_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable thymidylate kinase</fullName>
            <ecNumber evidence="1">2.7.4.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">dTMP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tmk</name>
        <name type="ordered locus">NP_2478A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>dTMP + ATP = dTDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:13517"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58369"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thymidylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.9" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322956.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IRB3"/>
    <dbReference type="SMR" id="Q3IRB3"/>
    <dbReference type="STRING" id="348780.NP_2478A"/>
    <dbReference type="EnsemblBacteria" id="CAI49330">
        <property type="protein sequence ID" value="CAI49330"/>
        <property type="gene ID" value="NP_2478A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703273"/>
    <dbReference type="KEGG" id="nph:NP_2478A"/>
    <dbReference type="eggNOG" id="arCOG01891">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049131_0_2_2"/>
    <dbReference type="OrthoDB" id="43083at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004798">
        <property type="term" value="F:dTMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006233">
        <property type="term" value="P:dTDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006227">
        <property type="term" value="P:dUDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01672">
        <property type="entry name" value="TMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00165">
        <property type="entry name" value="Thymidylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039430">
        <property type="entry name" value="Thymidylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018094">
        <property type="entry name" value="Thymidylate_kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00041">
        <property type="entry name" value="DTMP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10344">
        <property type="entry name" value="THYMIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10344:SF4">
        <property type="entry name" value="UMP-CMP KINASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02223">
        <property type="entry name" value="Thymidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000023231" description="Probable thymidylate kinase">
        <location>
            <begin position="1"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00165"/>
        </source>
    </evidence>
    <sequence length="196" mass="21682" checksum="F3C7A12E5C3CF07B" modified="2005-11-08" version="1">MLITLEGIDGSGKSTAHAALRERLTAEETTFTREPTDSWYGSAVERSIGDADADPLAELFLYTADHADHLSRVVRPALEAGEPVVSDRYSDSRYAYQGATLEGVIDEPMAYVKRVHEPFTRPPDATLYFDVPPTVGAERAGATNKFEAADYLESVRENYERLIEAEPERFHRIDATQSESAVVEAAVETVETLLSR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-07-10" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IRM1</accession>
    <name>Y2262_NATPD</name>
    <protein>
        <recommendedName>
            <fullName>Putative heme-binding protein NP_2262A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hemQ</name>
        <name type="ordered locus">NP_2262A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the ChdC family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322849.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IRM1"/>
    <dbReference type="SMR" id="Q3IRM1"/>
    <dbReference type="STRING" id="348780.NP_2262A"/>
    <dbReference type="EnsemblBacteria" id="CAI49222">
        <property type="protein sequence ID" value="CAI49222"/>
        <property type="gene ID" value="NP_2262A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701394"/>
    <dbReference type="KEGG" id="nph:NP_2262A"/>
    <dbReference type="eggNOG" id="arCOG03031">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_470618_0_0_2"/>
    <dbReference type="OrthoDB" id="8690at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1030">
        <property type="entry name" value="Apc35880, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007138">
        <property type="entry name" value="ABM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010644">
        <property type="entry name" value="ChdC/CLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007124">
        <property type="entry name" value="PRK09565.1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008913">
        <property type="entry name" value="PRK12276.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36843:SF1">
        <property type="entry name" value="COPROHEME DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36843">
        <property type="entry name" value="HEME-DEPENDENT PEROXIDASE YWFI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03992">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06778">
        <property type="entry name" value="Chlor_dismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51725">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0349">Heme</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000294063" description="Putative heme-binding protein NP_2262A">
        <location>
            <begin position="1"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="domain" description="ABM">
        <location>
            <begin position="510"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="261"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="270"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="1">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0000312" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49222.1"/>
        </source>
    </evidence>
    <sequence length="600" mass="66904" checksum="0FAF6A25F05AE5D4" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IRT0</accession>
    <name>KAD6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative adenylate kinase</fullName>
            <shortName evidence="1">AK</shortName>
            <ecNumber evidence="1">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP-AMP transphosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">adk2</name>
        <name type="ordered locus">NP_2142A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Broad-specificity nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Also has ATPase activity. Involved in the late maturation steps of the 30S ribosomal particles, specifically 16S rRNA maturation. While NMP activity is not required for ribosome maturation, ATPase activity is. Associates transiently with small ribosomal subunit protein uS11. ATP hydrolysis breaks the interaction with uS11. May temporarily remove uS11 from the ribosome to enable a conformational change of the ribosomal RNA that is needed for the final maturation step of the small ribosomal subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with uS11. Not a structural component of 40S pre-ribosomes, but transiently interacts with them by binding to uS11.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the adenylate kinase family. AK6 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322790.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IRT0"/>
    <dbReference type="SMR" id="Q3IRT0"/>
    <dbReference type="STRING" id="348780.NP_2142A"/>
    <dbReference type="EnsemblBacteria" id="CAI49162">
        <property type="protein sequence ID" value="CAI49162"/>
        <property type="gene ID" value="NP_2142A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701362"/>
    <dbReference type="KEGG" id="nph:NP_2142A"/>
    <dbReference type="eggNOG" id="arCOG01038">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079096_0_1_2"/>
    <dbReference type="OrthoDB" id="8730at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00039">
        <property type="entry name" value="Adenylate_kinase_AK6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020618">
        <property type="entry name" value="Adenyl_kinase_AK6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12595:SF0">
        <property type="entry name" value="ADENYLATE KINASE ISOENZYME 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12595">
        <property type="entry name" value="POS9-ACTIVATING FACTOR FAP7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13238">
        <property type="entry name" value="AAA_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000003093" description="Putative adenylate kinase">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="NMP" evidence="1">
        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="1">
        <location>
            <begin position="97"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49162.1"/>
        </source>
    </evidence>
    <sequence length="168" mass="17825" checksum="2E2D717A9409E976" modified="2005-11-08" version="1">MRVAVTGTPGTGKTTATEAVETALDVIHLNERIREEGLDAGRDEERDSLVADLDAVEAALAGEDDALIESHLAHHVDADRVVVLRCRPDILQERLVDRGEPAAKAAENAEAEALDVVLSEAVAEHGRESVYEIDTTEASPSAVAEAIEAVIDGERAPAVGTVDFTEHL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-03" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IRU8</accession>
    <name>CHED_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable chemoreceptor glutamine deamidase CheD</fullName>
            <ecNumber evidence="1">3.5.1.44</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cheD</name>
        <name type="ordered locus">NP_2106A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probably deamidates glutamine residues to glutamate on methyl-accepting chemotaxis receptors (MCPs), playing an important role in chemotaxis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutaminyl-[protein] + H2O = L-glutamyl-[protein] + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16441"/>
            <dbReference type="Rhea" id="RHEA-COMP:10207"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="EC" id="3.5.1.44"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CheD family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.44" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322772.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IRU8"/>
    <dbReference type="SMR" id="Q3IRU8"/>
    <dbReference type="STRING" id="348780.NP_2106A"/>
    <dbReference type="EnsemblBacteria" id="CAI49144">
        <property type="protein sequence ID" value="CAI49144"/>
        <property type="gene ID" value="NP_2106A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702601"/>
    <dbReference type="KEGG" id="nph:NP_2106A"/>
    <dbReference type="eggNOG" id="arCOG02380">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087854_2_0_2"/>
    <dbReference type="OrthoDB" id="10499at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050568">
        <property type="term" value="F:protein-glutamine glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16352">
        <property type="entry name" value="CheD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01440">
        <property type="entry name" value="CheD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038592">
        <property type="entry name" value="CheD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005659">
        <property type="entry name" value="Chemorcpt_Glu_NH3ase_CheD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011324">
        <property type="entry name" value="Cytotoxic_necrot_fac-like_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35147">
        <property type="entry name" value="CHEMORECEPTOR GLUTAMINE DEAMIDASE CHED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35147:SF1">
        <property type="entry name" value="CHEMORECEPTOR GLUTAMINE DEAMIDASE CHED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03975">
        <property type="entry name" value="CheD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64438">
        <property type="entry name" value="CNF1/YfiH-like putative cysteine hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0145">Chemotaxis</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000251095" description="Probable chemoreceptor glutamine deamidase CheD">
        <location>
            <begin position="1"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01440"/>
        </source>
    </evidence>
    <sequence length="167" mass="17184" checksum="354160BCDDB2FFE2" modified="2005-11-08" version="1">MAALPAESDTSERIKVSIADYAVAEAGTISTSGLGSCLGLAIYDPEAGVSALVHPMLPRRDGNDDRPPERFVDSGIDVVVDALLEHGASKASLRAKIAGGAAVVDFGSDDGDSIGDRNIEVAREELSDRGIELVGEEVGGDCGRTVKVDAATGDVNVSRTDGEETTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IS06</accession>
    <name>Q3IS06_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphate-specific transport system accessory protein PhoU</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">phoU2</name>
        <name evidence="9" type="synonym">abc14r2</name>
        <name evidence="9" type="ordered locus">NP_1986A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Plays a role in the regulation of phosphate uptake.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the PhoU family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322714.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IS06"/>
    <dbReference type="STRING" id="348780.NP_1986A"/>
    <dbReference type="EnsemblBacteria" id="CAI49084">
        <property type="protein sequence ID" value="CAI49084"/>
        <property type="gene ID" value="NP_1986A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702415"/>
    <dbReference type="KEGG" id="nph:NP_1986A"/>
    <dbReference type="eggNOG" id="arCOG00232">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078518_3_0_2"/>
    <dbReference type="OrthoDB" id="7738at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:intracellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045936">
        <property type="term" value="P:negative regulation of phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.220:FF:000004">
        <property type="entry name" value="Phosphate-specific transport system accessory protein PhoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="entry name" value="Phosphate transport system protein phou homolog 2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028366">
        <property type="entry name" value="PhoU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026022">
        <property type="entry name" value="PhoU_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02135">
        <property type="entry name" value="phoU_full"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930">
        <property type="entry name" value="PHOSPHATE-SPECIFIC TRANSPORT SYSTEM ACCESSORY PROTEIN PHOU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930:SF3">
        <property type="entry name" value="PHOSPHATE-SPECIFIC TRANSPORT SYSTEM ACCESSORY PROTEIN PHOU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01895">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003107">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109755">
        <property type="entry name" value="PhoU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PhoU" evidence="8">
        <location>
            <begin position="20"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="PhoU" evidence="8">
        <location>
            <begin position="121"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR003107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI49084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="226" mass="25705" checksum="63B13A9A7206460B" modified="2005-11-08" version="1">MARKGYQQQLNELRENVLYMSEIVLERVRRGLRALETKDESLAREVIEGDHEINELYLELEQSCIELFALQQPVASDLRFIAASFKIITDLERVADLATNLGDYTLQSSRDLYPDVDIQGIGDAVIEMVEDAMEAYAEEDSEACFAISDRDDEVDSRCEDASNTVVRDLIETELDETSSEGDIESLLREVSRLLLTIRDLERIGDHAVNIAARTLYMVENSDELIY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-12-06" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ISA5</accession>
    <name>RFCL_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Replication factor C large subunit</fullName>
            <shortName evidence="1">RFC large subunit</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Clamp loader large subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rfcL</name>
        <name type="synonym">rfcB</name>
        <name type="ordered locus">NP_1782A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heteromultimer composed of small subunits (RfcS) and large subunits (RfcL).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the activator 1 small subunits family. RfcL subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322615.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ISA5"/>
    <dbReference type="SMR" id="Q3ISA5"/>
    <dbReference type="STRING" id="348780.NP_1782A"/>
    <dbReference type="EnsemblBacteria" id="CAI48982">
        <property type="protein sequence ID" value="CAI48982"/>
        <property type="gene ID" value="NP_1782A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703101"/>
    <dbReference type="KEGG" id="nph:NP_1782A"/>
    <dbReference type="eggNOG" id="arCOG00470">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027255_1_0_2"/>
    <dbReference type="OrthoDB" id="8658at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01508">
        <property type="entry name" value="RfcL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023935">
        <property type="entry name" value="Rep_factor-C_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003228">
        <property type="entry name" value="PRK04195.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003229">
        <property type="entry name" value="PRK04195.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389">
        <property type="entry name" value="CHROMOSOME TRANSMISSION FIDELITY FACTOR 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389:SF6">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21960">
        <property type="entry name" value="RCF1-5-like_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000135958" description="Replication factor C large subunit">
        <location>
            <begin position="1"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="415"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="430"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="451"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="44"/>
            <end position="51"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="483" mass="53153" checksum="1B9659185F183982" modified="2005-11-08" version="1">MSDWTEAYRPTTLSEVRGNNKARDAFEEWAKAWEDHREAVILHGSPGVGKTSAAHALANDMGWPVLEMNASDARTKDEIERFAGRAASNATLGGGRQLIILDEADNLHQHKDRGGAAAMTRLVKDATQPVVLIANDYYEMSSGLRSACRDVEFRDVSARSIVPVLRDICRQENVEFDEDVLQEIAEANRGDLRGAVKDLQARERDGEIKPEGSEGSRDRTEDIFAFLDAVLKEESAEEALQTAYAVDETPDNLLQWIEDKVPKVYEGDELADAYEHLADADVWLGRVRATQNYSYWRYATDNVAAGVAAVRQEDRGGWTRYGGAPYRSSRDSTRDYIATRIAESAGVSTATARREILPYLSAMTHHCNNRELTVRMTARYELDAEHVAFITGSGKTTNKVQGIVEDAETRRETAAVDHGGGIFAPAVDDAQSDTESDDDDDGDTLAAFGADEPKEESVNREQSDGTADAEESDDGQAGLSDFM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ISB3</accession>
    <name>PHS_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative pterin-4-alpha-carbinolamine dehydratase</fullName>
            <shortName evidence="1">PHS</shortName>
            <ecNumber evidence="1">4.2.1.96</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">4-alpha-hydroxy-tetrahydropterin dehydratase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Pterin carbinolamine dehydratase</fullName>
            <shortName evidence="1">PCD</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">NP_1766A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin = (6R)-L-erythro-6,7-dihydrobiopterin + H2O</text>
            <dbReference type="Rhea" id="RHEA:11920"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15642"/>
            <dbReference type="ChEBI" id="CHEBI:43120"/>
            <dbReference type="EC" id="4.2.1.96"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the pterin-4-alpha-carbinolamine dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.96" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322607.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ISB3"/>
    <dbReference type="SMR" id="Q3ISB3"/>
    <dbReference type="STRING" id="348780.NP_1766A"/>
    <dbReference type="EnsemblBacteria" id="CAI48974">
        <property type="protein sequence ID" value="CAI48974"/>
        <property type="gene ID" value="NP_1766A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701250"/>
    <dbReference type="KEGG" id="nph:NP_1766A"/>
    <dbReference type="eggNOG" id="arCOG02939">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081974_4_0_2"/>
    <dbReference type="OrthoDB" id="10495at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008124">
        <property type="term" value="F:4-alpha-hydroxytetrahydrobiopterin dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00488">
        <property type="entry name" value="PCD_DCoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.20">
        <property type="entry name" value="Transcriptional coactivator/pterin dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00434">
        <property type="entry name" value="Pterin_4_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036428">
        <property type="entry name" value="PCD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001533">
        <property type="entry name" value="Pterin_deHydtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002017">
        <property type="entry name" value="PRK00823.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12599">
        <property type="entry name" value="PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12599:SF0">
        <property type="entry name" value="PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01329">
        <property type="entry name" value="Pterin_4a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55248">
        <property type="entry name" value="PCD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000231482" description="Putative pterin-4-alpha-carbinolamine dehydratase">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00434"/>
        </source>
    </evidence>
    <sequence length="92" mass="10297" checksum="D908119D77F151DE" modified="2005-11-08" version="1">MSDRLTDDEIQAQLPAAWDRDGDEIVRTFEFESYLEGVGFASAAGGLAEEAFHHPEMTIGWREVEVRLTTHDAGGITQKDIDLAERFDELAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ISE4</accession>
    <name>Q3ISE4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lipoate-protein ligase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">lpl2</name>
        <name evidence="2" type="ordered locus">NP_1704A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322576.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ISE4"/>
    <dbReference type="STRING" id="348780.NP_1704A"/>
    <dbReference type="EnsemblBacteria" id="CAI48943">
        <property type="protein sequence ID" value="CAI48943"/>
        <property type="gene ID" value="NP_1704A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702891"/>
    <dbReference type="KEGG" id="nph:NP_1704A"/>
    <dbReference type="eggNOG" id="arCOG01941">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085363_1_0_2"/>
    <dbReference type="OrthoDB" id="192160at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21948">
        <property type="entry name" value="LplA-B_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="1">
        <location>
            <begin position="27"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="234" mass="24329" checksum="67F3C36AAE928B22" modified="2005-11-08" version="1">MHVVRGALDSADADRQVVQRLAESVASTGVPALRVWQPPKHVAFGRRDSAADGYEHARLAARERGYTPIQRRAGGTAVAYAPGTVAFAYAVSTATGRGGIEDRYRTATERLLAALASVGAAVRRGEPSATFCPGTHSLRADGKVAGLAQRVKEETALVGGYVVGTDRASAAVADVLEPVYRALGTPFDPTTVGSVEAAGGPASAGQVADAIEASFVDERPAKYRSAQELLDRSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IT98</accession>
    <name>Q3IT98_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cobalt-precorrin-8 methylmutase</fullName>
            <ecNumber evidence="6">5.4.99.60</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">cbiC</name>
        <name evidence="6" type="synonym">cobH</name>
        <name evidence="6" type="ordered locus">NP_1090A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CobH/CbiC family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.60" evidence="6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322272.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IT98"/>
    <dbReference type="STRING" id="348780.NP_1090A"/>
    <dbReference type="EnsemblBacteria" id="CAI48636">
        <property type="protein sequence ID" value="CAI48636"/>
        <property type="gene ID" value="NP_1090A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702568"/>
    <dbReference type="KEGG" id="nph:NP_1090A"/>
    <dbReference type="eggNOG" id="arCOG02247">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084703_1_0_2"/>
    <dbReference type="OrthoDB" id="24491at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043778">
        <property type="term" value="F:cobalt-precorrin-8 methylmutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016993">
        <property type="term" value="F:precorrin-8X methylmutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10230">
        <property type="entry name" value="Cobalamin biosynthesis CobH/CbiC, precorrin-8X methylmutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003722">
        <property type="entry name" value="Cbl_synth_CobH/CbiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036588">
        <property type="entry name" value="CobH/CbiC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43588">
        <property type="entry name" value="COBALT-PRECORRIN-8 METHYLMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43588:SF1">
        <property type="entry name" value="COBALT-PRECORRIN-8 METHYLMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02570">
        <property type="entry name" value="CbiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63965">
        <property type="entry name" value="Precorrin-8X methylmutase CbiC/CobH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="4 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cobalamin biosynthesis precorrin-8X methylmutase CobH/CbiC" evidence="5">
        <location>
            <begin position="23"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI48636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="229" mass="24141" checksum="7493242C786CB818" modified="2005-11-08" version="1">MTTDETFEEYADLGATTSEAMDIAETSMDRVHDLVPQETLADRMRAKSVHATGDPEFQHLMRFTGTDESEPVRNGARAVLDERPIVTDITMVKEGITGRGHNCPVRKAIGNGAELAAETGMTRTAASVLELDSDGVYDDAIATIGNAPTAALALADCIEQGTRPAVVVATPVGFVKAAESRKRLRAVCAEHGVPTVTNVGRRGGSGLAAGLTNELIHVASDARDGEVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETB8</accession>
    <name>A0A1U7ETB8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC-type transport system ATP-binding protein (Probable substrate macrolides)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">abc30a</name>
        <name evidence="5" type="ordered locus">NP_0100A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321780.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETB8"/>
    <dbReference type="STRING" id="348780.NP_0100A"/>
    <dbReference type="EnsemblBacteria" id="CAI48141">
        <property type="protein sequence ID" value="CAI48141"/>
        <property type="gene ID" value="NP_0100A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702454"/>
    <dbReference type="KEGG" id="nph:NP_0100A"/>
    <dbReference type="eggNOG" id="arCOG00922">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098796">
        <property type="term" value="C:membrane protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000032">
        <property type="entry name" value="Export ABC transporter ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015854">
        <property type="entry name" value="ABC_transpr_LolD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24220:SF86">
        <property type="entry name" value="ABC TRANSPORTER ABCH.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24220">
        <property type="entry name" value="IMPORT ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI48141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="218" mass="23344" checksum="AA7BFE03256F579A" modified="2017-05-10" version="1">MPAIELTGVTRRYEGGGETVVALDSVSLSVDRGAFVAIMGPSGSGKSTLLNVIGLLDAPDAGSVVVDAVDVTDLDTAERTQMRKETVGFVFQDFHLIPTLSAQENVVLPTIYDREPRQERARALLERVGLGDRLDHAPDELSGGQKQRVAIARSLINEPGIILADEPTGNLDRETGTRILETLTDVTDRGVSVVAVTHDPAVADHADRTIELRDGVIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETC8</accession>
    <name>A0A1U7ETC8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Prephenate dehydratase</fullName>
            <ecNumber evidence="8">4.2.1.51</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">pheA</name>
        <name evidence="8" type="ordered locus">NP_0106A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.51" evidence="8"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321783.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETC8"/>
    <dbReference type="STRING" id="348780.NP_0106A"/>
    <dbReference type="EnsemblBacteria" id="CAI48144">
        <property type="protein sequence ID" value="CAI48144"/>
        <property type="gene ID" value="NP_0106A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703024"/>
    <dbReference type="KEGG" id="nph:NP_0106A"/>
    <dbReference type="eggNOG" id="arCOG00255">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035008_0_2_2"/>
    <dbReference type="OrthoDB" id="8755at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004664">
        <property type="term" value="F:prephenate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009094">
        <property type="term" value="P:L-phenylalanine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04905">
        <property type="entry name" value="ACT_CM-PDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13630">
        <property type="entry name" value="PBP2_PDT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001086">
        <property type="entry name" value="Preph_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018528">
        <property type="entry name" value="Preph_deHydtase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008865">
        <property type="entry name" value="PRK11898.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21022">
        <property type="entry name" value="PREPHENATE DEHYDRATASE P PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21022:SF19">
        <property type="entry name" value="PREPHENATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00800">
        <property type="entry name" value="PDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00858">
        <property type="entry name" value="PREPHENATE_DEHYDR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51171">
        <property type="entry name" value="PREPHENATE_DEHYDR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="4 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0584">Phenylalanine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prephenate dehydratase" evidence="6">
        <location>
            <begin position="2"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="7">
        <location>
            <begin position="183"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI48144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="267" mass="28680" checksum="B624CE56546FADAE" modified="2017-05-10" version="1">MKALTLGPEGTYSHRAAKAVTDDIEFVESVTGIVEGVAGGEYERGVVPIENSIEGSVTESLDALADRNVAIVREIVTPIQHALLAQASEFDIVASHPQALAQCREFLSAEYPSAQTEAVASTARGVERAREDPSVAAIGHPDTAGDGLQVLARDIQDSTSNATRFVVLAPETERSEAGGKSTIIVYPNTNHPGLLLELLEPFAERDINLSRVESRPSGERLGDYVFHVDFAAGLYEERTQNALEAVRDIAGEGWVRVLGSYDTEHVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETD4</accession>
    <name>A0A1U7ETD4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable sugar dehydrogenase (Homolog to aldose sugar dehydrogenase)</fullName>
            <ecNumber evidence="2">1.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0126A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321793.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETD4"/>
    <dbReference type="STRING" id="348780.NP_0126A"/>
    <dbReference type="EnsemblBacteria" id="CAI48154">
        <property type="protein sequence ID" value="CAI48154"/>
        <property type="gene ID" value="NP_0126A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700763"/>
    <dbReference type="KEGG" id="nph:NP_0126A"/>
    <dbReference type="eggNOG" id="arCOG02796">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012253_0_1_2"/>
    <dbReference type="OrthoDB" id="6744at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012938">
        <property type="entry name" value="Glc/Sorbosone_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011041">
        <property type="entry name" value="Quinoprot_gluc/sorb_DH_b-prop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19328">
        <property type="entry name" value="HEDGEHOG-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19328:SF13">
        <property type="entry name" value="HIPL1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07995">
        <property type="entry name" value="GSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50952">
        <property type="entry name" value="Soluble quinoprotein glucose dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose/Sorbosone dehydrogenase" evidence="1">
        <location>
            <begin position="79"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="445" mass="48291" checksum="8E96AD20406712ED" modified="2017-05-10" version="1">MRRPHSRRQFLVAAGLTVTAAAAGCFDSASEPTGEEPVEADHDLSVDHDIESWDRYDPDWTVPDASPGALEAETVVENLEIPWDLSFASDGECFLTERVGRISRYDGGDLDAVTEPANVIDHATAIDHGDEGGWWATGGEGGLLGNALHPNYPEVPVLYAVYTYEVGDDYRNRLVYFDLAGEEPEETIIIDDIPGDSFHNGSRLVFGPRNYLWVTTGDAGDEQLSADPDRLAGKVLRLNPDGTAPASNPDVGDPRVYSYGHRNAQGLSFMPDGTPIASEHGPAARDEVQIIGPGEDHGWPAVRNGPGVNDEYERYDAADGVTSPLVNTGMEETWAPSGAVFYTGSEVPSLRNRFLVGGLGSQTLYVVTVGESVPDIGGKRYDAPWLHPSYEAVVHERFEDELGRIRHVEQGPDGELYAVTSNRDGRADGPFPTESDDRLVRIVDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETD7</accession>
    <name>A0A1U7ETD7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Probable coiled coil protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0146A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321803.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETD7"/>
    <dbReference type="STRING" id="348780.NP_0146A"/>
    <dbReference type="EnsemblBacteria" id="CAI48164">
        <property type="protein sequence ID" value="CAI48164"/>
        <property type="gene ID" value="NP_0146A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700771"/>
    <dbReference type="KEGG" id="nph:NP_0146A"/>
    <dbReference type="HOGENOM" id="CLU_1485934_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="181" mass="18018" checksum="268CA4A41E03AD83" modified="2017-05-10" version="1">MATASGLLGSLCEAQRQAIATATDITNSGVDAQRQTADAVAATADAAAAVGAAGVSTTRAAADVATATMPLLDDSLERLDRRLDAAAAAEMALFEAGGVLAERSADTVDALNESTRDTAETCSDAALATHEAGETTAGAVTARLGETAAIMAIVAAVDGIDLIDRPAERFEDLAVDGVPDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETE0</accession>
    <name>A0A1U7ETE0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0150A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321805.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETE0"/>
    <dbReference type="STRING" id="348780.NP_0150A"/>
    <dbReference type="EnsemblBacteria" id="CAI48166">
        <property type="protein sequence ID" value="CAI48166"/>
        <property type="gene ID" value="NP_0150A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700773"/>
    <dbReference type="KEGG" id="nph:NP_0150A"/>
    <dbReference type="eggNOG" id="arCOG04680">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134204_0_0_2"/>
    <dbReference type="OrthoDB" id="247846at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055686">
        <property type="entry name" value="DUF7262"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23923">
        <property type="entry name" value="DUF7262"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="135" mass="14329" checksum="7F7525A5C2F22B54" modified="2017-05-10" version="1">MPDRGQLSLSIVEAGVGVVFVLAVALGFALGVPAPDTETPQLDAYADDVATVLANEPPRHGGETRLEEVTRSPAAFDRERDALESRVDRLLSANLMYRLETPHGAVGYERPAGSPAGQATAPTAGGEVRVWVWHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETG8</accession>
    <name>A0A1U7ETG8_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable beta-carotene 15,15'-dioxygenase</fullName>
            <ecNumber evidence="1">1.13.11.63</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">brp</name>
        <name evidence="2" type="ordered locus">NP_0206A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the cleavage of beta-carotene at its central double bond (15,15') to yield two molecules of all-trans-retinal.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>all-trans-beta-carotene + O2 = 2 all-trans-retinal</text>
            <dbReference type="Rhea" id="RHEA:32887"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17579"/>
            <dbReference type="ChEBI" id="CHEBI:17898"/>
            <dbReference type="EC" id="1.13.11.63"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Brp/Blh beta-carotene diooxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.63" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321833.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETG8"/>
    <dbReference type="STRING" id="348780.NP_0206A"/>
    <dbReference type="DNASU" id="3702557"/>
    <dbReference type="EnsemblBacteria" id="CAI48194">
        <property type="protein sequence ID" value="CAI48194"/>
        <property type="gene ID" value="NP_0206A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702557"/>
    <dbReference type="KEGG" id="nph:NP_0206A"/>
    <dbReference type="eggNOG" id="arCOG02946">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068196_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003834">
        <property type="term" value="F:beta-carotene 15,15'-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010436">
        <property type="term" value="F:carotenoid dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016121">
        <property type="term" value="P:carotene catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02093">
        <property type="entry name" value="Beta_carotene_diox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022270">
        <property type="entry name" value="Blh_diox"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03753">
        <property type="entry name" value="blh_monoox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15461">
        <property type="entry name" value="BCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="1" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="223"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="280"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="311"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="347" mass="36717" checksum="76CEE091C627CD9F" modified="2017-05-10" version="1">MATVESNLRRLGTSRSRRLLSGSRIALVVLIGWFLLARWAGISLSMQTQAAVYLFGMVALNLPYGGYEHMVNLDPRELSFGLRYIAAYLALVVGFVAVFFVAPVVGLALAIAVAVAKGGHGGLRVLDATVGTSHLKTTAQRALAAFVRGGTVMIVPLFFWPGDFAAFSTFMVDMFEMGAFASVAPHFDALRYGLGAVFAVAAVSHVAVGYLRHETLSTWALDAGETALLIAYFAVVPVVVAVGLYFPLWYSARQSARIAAVESDTLDSGPDSSRTAPGTAVAAFVGGAIATFALAGLLFVLLPNPMAGGTLLISGVAFFTVFISIIALPHVVVGSWFDTDRGIWYVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETM5</accession>
    <name>A0A1U7ETM5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Translation initiation factor 1A</fullName>
            <shortName evidence="2">aIF-1A</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">tif1A2</name>
        <name evidence="2" type="synonym">eif1a</name>
        <name evidence="6" type="ordered locus">NP_0334A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the eIF-1A family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48258.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939401.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETM5"/>
    <dbReference type="STRING" id="348780.NP_0334A"/>
    <dbReference type="EnsemblBacteria" id="CAI48258">
        <property type="protein sequence ID" value="CAI48258"/>
        <property type="gene ID" value="NP_0334A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703262"/>
    <dbReference type="KEGG" id="nph:NP_0334A"/>
    <dbReference type="eggNOG" id="arCOG01179">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109098_1_2_2"/>
    <dbReference type="OrthoDB" id="2586at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05793">
        <property type="entry name" value="S1_IF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00216">
        <property type="entry name" value="aIF_1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006196">
        <property type="entry name" value="RNA-binding_domain_S1_IF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001253">
        <property type="entry name" value="TIF_eIF-1A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00523">
        <property type="entry name" value="eIF-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003083">
        <property type="entry name" value="PRK04012.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003084">
        <property type="entry name" value="PRK04012.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003085">
        <property type="entry name" value="PRK04012.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21668">
        <property type="entry name" value="EIF-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01176">
        <property type="entry name" value="eIF-1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00652">
        <property type="entry name" value="eIF1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50832">
        <property type="entry name" value="S1_IF1_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 6" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="2 3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S1-like" evidence="5">
        <location>
            <begin position="6"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI48258.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="94" mass="11356" checksum="B0AB77360F4B18A0" modified="2017-05-10" version="1">MTEETQRKDLRMPDDSEMFATVTEMLGGQRVRLQCNDGKERMGRIPGRMRFRTWINKDDVVLIEPWDWQDEKADIEWRYEEADANQLRREGHIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETR7</accession>
    <name>A0A1U7ETR7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ASCH domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0472A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321963.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETR7"/>
    <dbReference type="STRING" id="348780.NP_0472A"/>
    <dbReference type="EnsemblBacteria" id="CAI48327">
        <property type="protein sequence ID" value="CAI48327"/>
        <property type="gene ID" value="NP_0472A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700868"/>
    <dbReference type="KEGG" id="nph:NP_0472A"/>
    <dbReference type="eggNOG" id="arCOG04714">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149288_0_0_2"/>
    <dbReference type="OrthoDB" id="359403at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06552">
        <property type="entry name" value="ASCH_yqfb_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="106" mass="11810" checksum="C8E998B0217350BF" modified="2017-05-10" version="1">MAEIDADALLPNDHVKTAVLDGDITQLSRGASNRYAEAGDTFELEGTTFEVTAVEERTLGDLTDDDARREGSPSLEAYRERMQRAHPGGFEWDDESDILTYRFEPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETZ4</accession>
    <name>A0A1U7ETZ4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DUF2070 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0618A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322036.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETZ4"/>
    <dbReference type="STRING" id="348780.NP_0618A"/>
    <dbReference type="EnsemblBacteria" id="CAI48400">
        <property type="protein sequence ID" value="CAI48400"/>
        <property type="gene ID" value="NP_0618A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700907"/>
    <dbReference type="KEGG" id="nph:NP_0618A"/>
    <dbReference type="eggNOG" id="arCOG04351">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_441213_0_0_2"/>
    <dbReference type="OrthoDB" id="8914at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019204">
        <property type="entry name" value="DUF2070_membrane"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09843">
        <property type="entry name" value="DUF2070"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="594"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2070" evidence="2">
        <location>
            <begin position="9"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="615" mass="62658" checksum="C3A8035CF0B60442" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU65</accession>
    <name>A0A1U7EU65_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Small CPxCG-related zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0788A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU65"/>
    <dbReference type="STRING" id="348780.NP_0788A"/>
    <dbReference type="EnsemblBacteria" id="CAI48485">
        <property type="protein sequence ID" value="CAI48485"/>
        <property type="gene ID" value="NP_0788A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_0788A"/>
    <dbReference type="eggNOG" id="arCOG06428">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_210071_1_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055985">
        <property type="entry name" value="DUF7563"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24444">
        <property type="entry name" value="DUF7563"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="52" mass="5803" checksum="B3FB05DFCBC3D2D0" modified="2017-05-10" version="1">MPECQNCGAFVTAAYARVFTPNGVENPRVCPQCEDKIRDGSDVREARSTRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU70</accession>
    <name>A0A1U7EU70_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Anthranilate synthase component II</fullName>
            <ecNumber evidence="2">4.1.3.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">pabA</name>
        <name evidence="11" type="synonym">trpG2</name>
        <name evidence="11" type="ordered locus">NP_0800A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21732"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="4.1.3.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.27" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322127.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU70"/>
    <dbReference type="STRING" id="348780.NP_0800A"/>
    <dbReference type="EnsemblBacteria" id="CAI48491">
        <property type="protein sequence ID" value="CAI48491"/>
        <property type="gene ID" value="NP_0800A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703418"/>
    <dbReference type="KEGG" id="nph:NP_0800A"/>
    <dbReference type="eggNOG" id="arCOG00086">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014340_1_2_2"/>
    <dbReference type="OrthoDB" id="3321at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004049">
        <property type="term" value="F:anthranilate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01743">
        <property type="entry name" value="GATase1_Anthranilate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000003">
        <property type="entry name" value="Anthranilate synthase component II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050472">
        <property type="entry name" value="Anth_synth/Amidotransfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006221">
        <property type="entry name" value="TrpG/PapA_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00566">
        <property type="entry name" value="trpG_papA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418:SF4">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="10">
        <location>
            <begin position="13"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI48491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="200" mass="21257" checksum="E542705AD71CA329" modified="2017-05-10" version="1">MTGEDAESQPTVLVIDNYDSFVYNLVQYVGTHANVVVRRNDEISAAGIGRLDPDGIVVSPGPGTPTDAGVSMPIFESLSYPTLGVCLGHQALCAANGAAVGHAPEVVHGKPSVINHDGRGIFASLPDRFEVGRYHSLSVEPGELPASLVETARTDDERGVVMGVRHRERPHIGVQFHPESILTDAGEQMIAAFCSQCKTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUK9</accession>
    <name>A0A1U7EUK9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ArsR family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1144A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322299.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUK9"/>
    <dbReference type="STRING" id="348780.NP_1144A"/>
    <dbReference type="EnsemblBacteria" id="CAI48663">
        <property type="protein sequence ID" value="CAI48663"/>
        <property type="gene ID" value="NP_1144A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701065"/>
    <dbReference type="KEGG" id="nph:NP_1144A"/>
    <dbReference type="eggNOG" id="arCOG01685">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145184_1_0_2"/>
    <dbReference type="OrthoDB" id="11368at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12840">
        <property type="entry name" value="HTH_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="114" mass="11986" checksum="2BBAF8E064A0612E" modified="2017-05-10" version="1">MCVNELVPTELDADSDRDLETVTIDDGDVLEALSSETARRVLQALDSGPAPASAVAETAGVSIQVAAYHLDRLETAGLVRTVATSYSEKGQPMDIYGLTTDSFVVELSGGDSPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUV9</accession>
    <name>A0A1U7EUV9_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sec-independent protein translocase protein TatC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tatC1</name>
        <name evidence="5" type="synonym">tatC</name>
        <name evidence="7" type="ordered locus">NP_1348A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Forms a complex with TatA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TatC family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322400.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUV9"/>
    <dbReference type="STRING" id="348780.NP_1348A"/>
    <dbReference type="EnsemblBacteria" id="CAI48765">
        <property type="protein sequence ID" value="CAI48765"/>
        <property type="gene ID" value="NP_1348A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703227"/>
    <dbReference type="KEGG" id="nph:NP_1348A"/>
    <dbReference type="eggNOG" id="arCOG01919">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031942_8_1_2"/>
    <dbReference type="OrthoDB" id="198870at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033281">
        <property type="term" value="C:TAT protein transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009977">
        <property type="term" value="F:proton motive force dependent protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043953">
        <property type="term" value="P:protein transport by the Tat complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00902">
        <property type="entry name" value="TatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002033">
        <property type="entry name" value="TatC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30371">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30371:SF0">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATC, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00902">
        <property type="entry name" value="TatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01840">
        <property type="entry name" value="TATCFAMILY"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="36"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="124"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="175"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="212"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="233"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="9"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="257" mass="28376" checksum="25B4EC0AD48CF118" modified="2017-05-10" version="1">MVETADGSYDADEGVVGDGPESDEEMPLADHIEEMVYRLGVVLVAVAVVAGVVFPFAAYIIDFLWYSVLPGGDIARPRVYHPLGLILARLKVATLAGFVIGLPVFVYQTYLFMRPGLYPQERRYYLASVPASLVLGIFGVLFAYFLILPAIFTYFLTYSQDAAVIAFGLTETFDLIVLMMGLFAAVFQIPLFVMLAIMMGLTTRAWLAEKRLYFWGGFLGVAFLFSPDPTGMAPFLVALTMIGLFEATLLVLKWVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUY5</accession>
    <name>A0A1U7EUY5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Globin family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">NP_1422A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the truncated hemoglobin family. Group I subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the truncated hemoglobin family. Group II subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322437.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUY5"/>
    <dbReference type="STRING" id="348780.NP_1422A"/>
    <dbReference type="EnsemblBacteria" id="CAI48802">
        <property type="protein sequence ID" value="CAI48802"/>
        <property type="gene ID" value="NP_1422A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701145"/>
    <dbReference type="KEGG" id="nph:NP_1422A"/>
    <dbReference type="eggNOG" id="arCOG06333">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103526_2_1_2"/>
    <dbReference type="OrthoDB" id="313164at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005344">
        <property type="term" value="F:oxygen carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00454">
        <property type="entry name" value="TrHb1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="entry name" value="Globins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044203">
        <property type="entry name" value="GlbO/GLB3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019795">
        <property type="entry name" value="Globin_bac-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001486">
        <property type="entry name" value="Hemoglobin_trunc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016339">
        <property type="entry name" value="Hemoglobin_trunc_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47366:SF2">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_37, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47366">
        <property type="entry name" value="TWO-ON-TWO HEMOGLOBIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01152">
        <property type="entry name" value="Bac_globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002030">
        <property type="entry name" value="Globin_Protozoa/Cyanobacteria"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="Globin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01213">
        <property type="entry name" value="GLOBIN_FAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0349">Heme</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0561">Oxygen transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI48802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="119" mass="13446" checksum="63262699A79583E0" modified="2017-05-10" version="1">MTETLYERLGGKESIAAVVDRFYDRMLDDERVSHHFEEVDMQQQRAQQTLFLCSLTGGPVEYTGEDMEPAHDHLGITEAEFDVTVDHLEAALEAFDVPERERTEVLEAVAGFRADIVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV36</accession>
    <name>A0A1U7EV36_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">FMN-binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1554A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322502.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV36"/>
    <dbReference type="STRING" id="348780.NP_1554A"/>
    <dbReference type="EnsemblBacteria" id="CAI48868">
        <property type="protein sequence ID" value="CAI48868"/>
        <property type="gene ID" value="NP_1554A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701180"/>
    <dbReference type="KEGG" id="nph:NP_1554A"/>
    <dbReference type="eggNOG" id="arCOG00512">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124266_1_1_2"/>
    <dbReference type="OrthoDB" id="953at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024747">
        <property type="entry name" value="Pyridox_Oxase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12900">
        <property type="entry name" value="Pyridox_ox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="148" mass="16999" checksum="AEE3A2DDD9772A67" modified="2017-05-10" version="1">MAIDRRTEMSSEETDEFLGTHETGVLSLARSDEPYSIPISYGYDQSDRQFYMRLISTPQSEKRQFLDSAPDARLVVYDEADDTYRSVIATGVLERVDPDELTADDIRQYGDARRPLFEIWPQQKGNLDIELYRLVPESLSGRVVVVSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV66</accession>
    <name>A0A1U7EV66_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PAC2 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1624A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322537.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV66"/>
    <dbReference type="STRING" id="348780.NP_1624A"/>
    <dbReference type="EnsemblBacteria" id="CAI48903">
        <property type="protein sequence ID" value="CAI48903"/>
        <property type="gene ID" value="NP_1624A"/>
    </dbReference>
    <dbReference type="GeneID" id="41345080"/>
    <dbReference type="KEGG" id="nph:NP_1624A"/>
    <dbReference type="eggNOG" id="arCOG00348">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075000_1_0_2"/>
    <dbReference type="OrthoDB" id="31247at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10900">
        <property type="entry name" value="PAC-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004426">
        <property type="entry name" value="MJ1210-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019151">
        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00162">
        <property type="entry name" value="proteasome assembly chaperone family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35610:SF7">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35610">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09754">
        <property type="entry name" value="PAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159659">
        <property type="entry name" value="Cgl1923-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="214"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="251" mass="27139" checksum="0494ACE297626C7C" modified="2017-05-10" version="1">MDEFDIERPADPELDNPVLIEGLPGVGHVGKLAAEHLLEEFDAELVRRIYSEHFPPQVTVEDGRTQLACAEVYAISAAERDCLVLTGDHQAADGPGHYRLTEAFLDVAAAFDVSRIFALGGVPTGELVETPDVIGAATTDDLIEELEAVDVEFRENEPAGGIVGVSGLLLGLGERRGYEAACLMGETSGYLVDPKSARVVLEALEETVPLDIGYDSLEERADEMEEVVKQIQQMEQNATAAPSEEDLRYIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV67</accession>
    <name>A0A1U7EV67_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CopG domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1568A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322509.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV67"/>
    <dbReference type="STRING" id="348780.NP_1568A"/>
    <dbReference type="EnsemblBacteria" id="CAI48875">
        <property type="protein sequence ID" value="CAI48875"/>
        <property type="gene ID" value="NP_1568A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701187"/>
    <dbReference type="KEGG" id="nph:NP_1568A"/>
    <dbReference type="eggNOG" id="arCOG04610">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2662192_0_0_2"/>
    <dbReference type="OrthoDB" id="298109at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055544">
        <property type="entry name" value="DUF7120"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23434">
        <property type="entry name" value="DUF7120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="82" mass="9169" checksum="D694C6B849D16925" modified="2017-05-10" version="1">MPEVEVSLPDRIDSEIARLVDQGEFLNREQAVEELLSMGVSAYAPSDSSDEPADEDIFTQMTDDQQDPAAREDDPQGDEYTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV79</accession>
    <name>A0A1U7EV79_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Homolog to virus protein HCTV2-VP27</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1650A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322550.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV79"/>
    <dbReference type="STRING" id="348780.NP_1650A"/>
    <dbReference type="EnsemblBacteria" id="CAI48916">
        <property type="protein sequence ID" value="CAI48916"/>
        <property type="gene ID" value="NP_1650A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701211"/>
    <dbReference type="KEGG" id="nph:NP_1650A"/>
    <dbReference type="HOGENOM" id="CLU_1485935_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50199">
        <property type="entry name" value="Staphylococcal nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="181" mass="19686" checksum="10DC4EC678FA0FA5" modified="2017-05-10" version="1">MTDGYTRRTLLAAVGATAVGGVTAGVLAQRTASGVSGATIQADGEVVLDDKNLYDATLVEIIDGDTADFIVDVGLNTKRKIRVRPTDIDTAEIFGVSEDSDEYARGRAQKQFVEELLSDADRLVFRSEEDTAAYNERFPHAFETTRSGKGGFGRWLGDINADGVWWSEAVLERWPDATYDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV98</accession>
    <name>A0A1U7EV98_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">phosphoribosylglycinamide formyltransferase 1</fullName>
            <ecNumber evidence="2">2.1.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">purNH</name>
        <name evidence="8" type="ordered locus">NP_1662A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.2" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322555.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV98"/>
    <dbReference type="SMR" id="A0A1U7EV98"/>
    <dbReference type="STRING" id="348780.NP_1662A"/>
    <dbReference type="EnsemblBacteria" id="CAI48922">
        <property type="protein sequence ID" value="CAI48922"/>
        <property type="gene ID" value="NP_1662A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703075"/>
    <dbReference type="KEGG" id="nph:NP_1662A"/>
    <dbReference type="eggNOG" id="arCOG02824">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016316_5_0_2"/>
    <dbReference type="OrthoDB" id="52603at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00126"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003937">
        <property type="term" value="F:IMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004643">
        <property type="term" value="F:phosphoribosylaminoimidazolecarboxamide formyltransferase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004644">
        <property type="term" value="F:phosphoribosylglycinamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd08645">
        <property type="entry name" value="FMT_core_GART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.20:FF:000001">
        <property type="entry name" value="Bifunctional purine biosynthesis protein PurH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.170">
        <property type="entry name" value="Formyl transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01930">
        <property type="entry name" value="PurN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AICAR_Tfase_dup_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004607">
        <property type="entry name" value="GART"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001555">
        <property type="entry name" value="GART_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002695">
        <property type="entry name" value="PurH-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002049">
        <property type="entry name" value="PRK00881.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00639">
        <property type="entry name" value="PurN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11692:SF0">
        <property type="entry name" value="BIFUNCTIONAL PURINE BIOSYNTHESIS PROTEIN ATIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11692">
        <property type="entry name" value="BIFUNCTIONAL PURINE BIOSYNTHESIS PROTEIN PURH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01808">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000414">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00798">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="Formyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00373">
        <property type="entry name" value="GART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="4" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Formyl transferase N-terminal" evidence="7">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI48922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="523" mass="56022" checksum="766D5D545A47CD0C" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVA5</accession>
    <name>A0A1U7EVA5_NATPD</name>
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        <submittedName>
            <fullName evidence="2">UspA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1710A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVA5"/>
    <dbReference type="STRING" id="348780.NP_1710A"/>
    <dbReference type="EnsemblBacteria" id="CAI48946">
        <property type="protein sequence ID" value="CAI48946"/>
        <property type="gene ID" value="NP_1710A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701232"/>
    <dbReference type="KEGG" id="nph:NP_1710A"/>
    <dbReference type="eggNOG" id="arCOG02053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_19_0_2"/>
    <dbReference type="OrthoDB" id="213488at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
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        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="1">
        <location>
            <begin position="2"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="122" mass="13075" checksum="3A397FCAD636CF28" modified="2017-05-10" version="1">MKVLLGIGGSDDSLLALEKAVDRAREAGDELTIAIVENPDSDRSPESIRDRVKSVLDESGFDAEVRMVSGHPGSQLLELAEREDFDRIALGGGETSPLGKVQLGSIAEFVLLNARTTVTLIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVD2</accession>
    <name>A0A1U7EVD2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ribonuclease H, type 1</fullName>
            <ecNumber evidence="2">3.1.26.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rnhA1</name>
        <name evidence="2" type="ordered locus">NP_1792A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.26.4" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322619.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVD2"/>
    <dbReference type="EnsemblBacteria" id="CAI48987">
        <property type="protein sequence ID" value="CAI48987"/>
        <property type="gene ID" value="NP_1792A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701255"/>
    <dbReference type="KEGG" id="nph:NP_1792A"/>
    <dbReference type="eggNOG" id="arCOG02941">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092679_0_0_2"/>
    <dbReference type="OrthoDB" id="333599at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="220" mass="24006" checksum="9402EE877FFC5041" modified="2017-05-10" version="1">MAAHRRPVLRNLFDDSPTPHIAHPPRTHQREFYVATDGSYRSDGSGGLGVVIETRGGERVARLSVPDTVPDNNVAEYRALHLGLDVLAARAPRTATVGVLVDHDDLAENVNRAALAGRGPDSRPPHPFEVPGRTAHHWRGIRARVAGFGELRAARIDSGENPAHPLANTPDQYAHVNRDVDRCVLPRDDRWQSPDDDRYPPPSRADRERTDGAAGGSASD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVD3</accession>
    <name>A0A1U7EVD3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Homolog to rhodopsin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">NP_1758A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal/bacterial/fungal opsin family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322603.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVD3"/>
    <dbReference type="STRING" id="348780.NP_1758A"/>
    <dbReference type="EnsemblBacteria" id="CAI48970">
        <property type="protein sequence ID" value="CAI48970"/>
        <property type="gene ID" value="NP_1758A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701246"/>
    <dbReference type="KEGG" id="nph:NP_1758A"/>
    <dbReference type="eggNOG" id="arCOG02810">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093717_0_0_2"/>
    <dbReference type="OrthoDB" id="330248at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001425">
        <property type="entry name" value="Arc/bac/fun_rhodopsins"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01036">
        <property type="entry name" value="Bac_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01021">
        <property type="entry name" value="Bac_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="163"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="233" mass="24575" checksum="7463745C965B1848" modified="2017-05-10" version="1">MLPTSTIYLGMVVALSLGAVVFAAVASRLSGTARRLGLIAAVPAAAMAVAYLGMGLEWLTIETEGREQSIMRFLGYTVALTAYTYLLARPVGLSRRKAVGLLAVLLVTLWSALATWLTTGAAESAITLLSISAYLFGVYLLFKPFSRVAAAAPGEARLLYGKLRNLFVLCWGGLLVASAISEQAIGLTDAFIGQFAASYIDLVLMLGIGGLVLFSRFVRDPTATERADATAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVF9</accession>
    <name>A0A1U7EVF9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Probable iron-sulfur protein (2Fe-2S)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">NP_1834A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322640.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVF9"/>
    <dbReference type="STRING" id="348780.NP_1834A"/>
    <dbReference type="EnsemblBacteria" id="CAI49008">
        <property type="protein sequence ID" value="CAI49008"/>
        <property type="gene ID" value="NP_1834A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701269"/>
    <dbReference type="KEGG" id="nph:NP_1834A"/>
    <dbReference type="eggNOG" id="arCOG02851">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055690_4_1_2"/>
    <dbReference type="OrthoDB" id="250454at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="6">
        <location>
            <begin position="41"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="120"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="143" mass="15498" checksum="9D6E9E1EDF1400A3" modified="2017-05-10" version="1">MVRYRLTDIETVREEGVWLFTGRVGGGEEREIIVVPCEDDSADSDVEAWENRCTHEDQRLYREDVGAVVRDGSVVCPKHGSAFDSCSGGCDNGPAAGSTLPSVDVAAEDGQVYLTDDNVSYIHAGPSDDDDDDEPGSTSHLRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVH6</accession>
    <name>A0A1U7EVH6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Acyl-CoA dehydrogenase</fullName>
            <ecNumber evidence="10">1.3.8.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">acd9</name>
        <name evidence="10" type="ordered locus">NP_1870A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.-" evidence="10"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322658.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVH6"/>
    <dbReference type="STRING" id="348780.NP_1870A"/>
    <dbReference type="EnsemblBacteria" id="CAI49026">
        <property type="protein sequence ID" value="CAI49026"/>
        <property type="gene ID" value="NP_1870A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702483"/>
    <dbReference type="KEGG" id="nph:NP_1870A"/>
    <dbReference type="eggNOG" id="arCOG01707">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_0_2_2"/>
    <dbReference type="OrthoDB" id="275197at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000002">
        <property type="entry name" value="Acyl-CoA dehydrogenase FadE19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000004">
        <property type="entry name" value="Acyl-CoA dehydrogenase FadE25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000001">
        <property type="entry name" value="Acyl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF12">
        <property type="entry name" value="ISOVALERYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016578">
        <property type="entry name" value="HsaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0274">FAD</keyword>
    <keyword evidence="3 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 6 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="9">
        <location>
            <begin position="4"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="8">
        <location>
            <begin position="119"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="7">
        <location>
            <begin position="225"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI49026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="374" mass="40950" checksum="E7EECF7CD77C75A7" modified="2017-05-10" version="1">MELTDEQRMIQETVREFVDEDVSENVESADKAQAFPEDIWDALAELDLTGLTVPERYGGFDADEVTYSIVNEELARGHLAVATALSVHGLATSCIRQFGSAEQQDRWLSEMVDGRPVGAFALSEPDAGSNPAEMSTEAVKDGDEYVINGKKQWITNGERSGVVILFAKTDRDDPNTVTQFLVPKDVEGLEVGKKEDKLGLRASDTTTLLFDDVRIPAENRLTEVGDGLQAAFSILTGGRIAIAAQAVGLAQAALDDAVEYANDREQFGKPLIEHQAIAHKLADMQTDIQAARLLTRDAARKNETGVDPKAASMAKYFASETAVDVANEAVQVHGGYGYTKDFDVERYYRDAKITTIYEGTSEIQKKVIARHLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVL3</accession>
    <name>A0A1U7EVL3_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Nitric oxide synthase oxygenase</fullName>
            <ecNumber evidence="3 10">1.14.14.47</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">nos</name>
        <name evidence="12" type="ordered locus">NP_1908A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the production of nitric oxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>3 reduced [flavodoxin] + 2 L-arginine + 4 O2 = 3 oxidized [flavodoxin] + 2 L-citrulline + 2 nitric oxide + 4 H2O + 5 H(+)</text>
            <dbReference type="Rhea" id="RHEA:52324"/>
            <dbReference type="Rhea" id="RHEA-COMP:10622"/>
            <dbReference type="Rhea" id="RHEA-COMP:10623"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16480"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="1.14.14.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="10">This protein is similar to the oxygenase domain of eukaryotic nitric oxide synthases but lacks the reductase domain which, in eukaryotes, is responsible for transfer of electrons to the ferric heme during nitric oxide synthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the NOS family. Bacterial NOS oxygenase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.47" evidence="3 10"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322677.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVL3"/>
    <dbReference type="SMR" id="A0A1U7EVL3"/>
    <dbReference type="STRING" id="348780.NP_1908A"/>
    <dbReference type="EnsemblBacteria" id="CAI49045">
        <property type="protein sequence ID" value="CAI49045"/>
        <property type="gene ID" value="NP_1908A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702982"/>
    <dbReference type="KEGG" id="nph:NP_1908A"/>
    <dbReference type="eggNOG" id="arCOG14711">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040293_0_0_2"/>
    <dbReference type="OrthoDB" id="234138at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004517">
        <property type="term" value="F:nitric-oxide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006809">
        <property type="term" value="P:nitric oxide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00575">
        <property type="entry name" value="NOS_oxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.340.10">
        <property type="entry name" value="Nitric Oxide Synthase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1230.10">
        <property type="entry name" value="Nitric Oxide Synthase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.440.10">
        <property type="entry name" value="Nitric Oxide Synthase,Heme Domain,Chain A domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017142">
        <property type="entry name" value="Nitric_oxide_synthase_Oase-su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050607">
        <property type="entry name" value="NOS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044943">
        <property type="entry name" value="NOS_dom_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044940">
        <property type="entry name" value="NOS_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044944">
        <property type="entry name" value="NOS_dom_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004030">
        <property type="entry name" value="NOS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036119">
        <property type="entry name" value="NOS_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43410:SF1">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43410">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE OXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02898">
        <property type="entry name" value="NO_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037219">
        <property type="entry name" value="NOS_oxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56512">
        <property type="entry name" value="Nitric oxide (NO) synthase oxygenase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS60001">
        <property type="entry name" value="NOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0349">Heme</keyword>
    <keyword evidence="8 10" id="KW-0408">Iron</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitric oxide synthase (NOS)" evidence="11">
        <location>
            <begin position="71"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR037219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS60001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI49045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="378" mass="43781" checksum="03E15471E3AB955C" modified="2017-05-10" version="1">MHEPPQEYSREERFAEAKSFIEQCYTELGRESEIDDRLAEIEAEIERRNHYEHTPEELEHGAKMAWRNSNRCIGRLFWQQLNVADERDCETAEAVHEACCRHLEKARNGGDIKPLITVFKPMVDGDRQVRIWNYELLRYAGYRTDDGIVGDPDEIELTDYCQSRGWEGDGTDFDILPHVIQMGDDEPAIFEVPDSAVGEVPLRHPDYEWFEDLGLRWYDVPVVSNMRLELGGIHYTAAPFSGWYVSTEIGARNLADEDRYDMLPTVAERLGLDTDDNRSLWKDEALVELNRAVLHSYEEAGVTIVDHHTVTDQFEQFEQNEAEADREVTGDRSWLLPPMSSATTHVFSNDYDDRIEKPNFFYQAAPEPLSERRESLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVR4</accession>
    <name>A0A1U7EVR4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC-type transport system permease protein (Probable substrate heme)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ccmC</name>
        <name evidence="9" type="synonym">abc29p2</name>
        <name evidence="9" type="ordered locus">NP_2048A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CcmC/CycZ/HelC family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322744.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVR4"/>
    <dbReference type="STRING" id="348780.NP_2048A"/>
    <dbReference type="EnsemblBacteria" id="CAI49115">
        <property type="protein sequence ID" value="CAI49115"/>
        <property type="gene ID" value="NP_2048A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702453"/>
    <dbReference type="KEGG" id="nph:NP_2048A"/>
    <dbReference type="eggNOG" id="arCOG00267">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066538_1_0_2"/>
    <dbReference type="OrthoDB" id="148358at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015232">
        <property type="term" value="F:heme transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002541">
        <property type="entry name" value="Cyt_c_assembly"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003557">
        <property type="entry name" value="Cyt_c_biogenesis_CcmC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045062">
        <property type="entry name" value="Cyt_c_biogenesis_CcsA/CcmC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30071:SF1">
        <property type="entry name" value="CYTOCHROME B_B6 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30071">
        <property type="entry name" value="HEME EXPORTER PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01578">
        <property type="entry name" value="Cytochrom_C_asm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01386">
        <property type="entry name" value="CCMCBIOGNSIS"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="54"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="93"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="128"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="157"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="195"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c assembly protein" evidence="8">
        <location>
            <begin position="53"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI49115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="248" mass="27475" checksum="C95DC41C0AF4896C" modified="2017-05-10" version="1">MDTDRRLSVRVLRRLFHSAVPGYLALGFGLASLVLVFGFASASMYGVDHGINLIAYWHVPLAIVGGLALTVTFVGCVLYLVTSTRFWEQLAHASGELGFIFATLTLVTGSAWGRIIWNTWWDWSDIRLVTFLFVWFIYAGYLIIYSGAGDGGRVSRLASVYGVVGFITVPISYASTRLWTAQLHAPSIGNPDAEANITASVLFVSLLAIFFLYVYLLGNRVRLHELTSRLDEMKDRTRTKTHKPTGGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVW5</accession>
    <name>A0A1U7EVW5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Probable oxidoreductase (Short-chain dehydrogenase family)</fullName>
            <ecNumber evidence="5">1.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_2222A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322829.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVW5"/>
    <dbReference type="STRING" id="348780.NP_2222A"/>
    <dbReference type="EnsemblBacteria" id="CAI49202">
        <property type="protein sequence ID" value="CAI49202"/>
        <property type="gene ID" value="NP_2222A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701381"/>
    <dbReference type="KEGG" id="nph:NP_2222A"/>
    <dbReference type="eggNOG" id="arCOG01259">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_2_2"/>
    <dbReference type="OrthoDB" id="7442at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032787">
        <property type="term" value="P:monocarboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000173">
        <property type="entry name" value="3-oxoacyl-[acyl-carrier protein] reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050259">
        <property type="entry name" value="SDR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42879">
        <property type="entry name" value="3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42879:SF2">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] REDUCTASE FABG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketoreductase" evidence="4">
        <location>
            <begin position="8"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="258" mass="27043" checksum="7CC5572AF1032816" modified="2017-05-10" version="1">MDLGLDGRTALVTGGGGRIGGEDCRILAAEGAEVVALDVNLGAAENVVEEIEADGGTAHAVECDLTDRADVEDTIAALEAETGGIDVLVNNAGMVDARDRIEEFDDEIWDRDISVNLTGAYNVTRAVYPNMKERGWGRIINMSSMAGWQGGFGQASYAATKAALIGFGKTLALEGAQHGVTSNIIAPSIVVGQLADLPIDQLETVDEHFARIAKATPMRRLGTEADVANLVAYLASEQADYITGQVVGVTGGIDLFSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVY0</accession>
    <name>A0A1U7EVY0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N5-carboxyaminoimidazole ribonucleotide mutase</fullName>
            <shortName evidence="3">N5-CAIR mutase</shortName>
            <ecNumber evidence="3">5.4.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">5-(carboxyamino)imidazole ribonucleotide mutase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3 5" type="primary">purE</name>
        <name evidence="5" type="ordered locus">NP_2290A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the conversion of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to 4-carboxy-5-aminoimidazole ribonucleotide (CAIR).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole + H(+) = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate</text>
            <dbReference type="Rhea" id="RHEA:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58730"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="EC" id="5.4.99.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AIR carboxylase family. Class I subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.18" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322863.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVY0"/>
    <dbReference type="STRING" id="348780.NP_2290A"/>
    <dbReference type="EnsemblBacteria" id="CAI49236">
        <property type="protein sequence ID" value="CAI49236"/>
        <property type="gene ID" value="NP_2290A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703070"/>
    <dbReference type="KEGG" id="nph:NP_2290A"/>
    <dbReference type="eggNOG" id="arCOG02464">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094982_1_1_2"/>
    <dbReference type="OrthoDB" id="9473at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00943"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034023">
        <property type="term" value="F:5-(carboxyamino)imidazole ribonucleotide mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01929">
        <property type="entry name" value="PurE_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033747">
        <property type="entry name" value="PurE_ClassI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000031">
        <property type="entry name" value="PurE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024694">
        <property type="entry name" value="PurE_prokaryotes"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01162">
        <property type="entry name" value="purE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23046:SF2">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23046">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00731">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01001">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52255">
        <property type="entry name" value="N5-CAIR mutase (phosphoribosylaminoimidazole carboxylase, PurE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1 3" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PurE" evidence="4">
        <location>
            <begin position="28"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="206" mass="22284" checksum="D8227BFBCF5494DB" modified="2017-05-10" version="1">MTRSEEVAALIDLFEAEAERDRSDAETPDIGIVMGSDSDLDTMYGAYEALTELGFEEVTDYDDPPESRFTFETFVVSAHRTPELMYAYGETARDRGLDVIIAGAGGKSADLPNMTASIAYPLPVVGVPVHEKSVSSVIGMPTGAPIVAVDAGKSFNAALSAVQMLARGHPELEARLEAYHDDLQDNVGAVSKRLHEQGTDRFRESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW50</accession>
    <name>A0A1U7EW50_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10 11">Quinolinate synthase</fullName>
            <ecNumber evidence="2 10 11">2.5.1.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 12" type="primary">nadA</name>
        <name evidence="12" type="ordered locus">NP_2418A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the condensation of iminoaspartate with dihydroxyacetone phosphate to form quinolinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>iminosuccinate + dihydroxyacetone phosphate = quinolinate + phosphate + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25888"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29959"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
            <dbReference type="EC" id="2.5.1.72"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="10">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from iminoaspartate: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the quinolinate synthase family. Type 3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.72" evidence="2 10 11"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322926.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW50"/>
    <dbReference type="STRING" id="348780.NP_2418A"/>
    <dbReference type="EnsemblBacteria" id="CAI49300">
        <property type="protein sequence ID" value="CAI49300"/>
        <property type="gene ID" value="NP_2418A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702961"/>
    <dbReference type="KEGG" id="nph:NP_2418A"/>
    <dbReference type="eggNOG" id="arCOG04459">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047382_2_0_2"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00327"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008987">
        <property type="term" value="F:quinolinate synthetase A activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034628">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-aspartate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10800">
        <property type="entry name" value="NadA-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00569">
        <property type="entry name" value="NadA_type3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003473">
        <property type="entry name" value="NadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036094">
        <property type="entry name" value="NadA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023515">
        <property type="entry name" value="Quinolinate_synth_A_type3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00550">
        <property type="entry name" value="nadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006883">
        <property type="entry name" value="PRK09375.2-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30573:SF0">
        <property type="entry name" value="QUINOLINATE SYNTHASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30573">
        <property type="entry name" value="QUINOLINATE SYNTHETASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02445">
        <property type="entry name" value="NadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142754">
        <property type="entry name" value="NadA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10" id="KW-0408">Iron</keyword>
    <keyword evidence="9 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10 12" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="152"/>
            <end position="154"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="266"/>
            <end position="268"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="NCBIfam" id="TIGR00550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI49300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="378" mass="42588" checksum="730BF7B74E0281E8" modified="2017-05-10" version="1">MPEMETVELETDLSLFKYDNLEQLPETYRELSEEERTERIEAAKAELGDDLVVLGHNYQRREIVDHADFIGDSYQLSKRAAEADAEYVVFGGVTFMAESADIITDDDQTVILPSMEASCPMAGMAEALQVDAAWAELTAAVDDDTDIIPITYMNSYADLKAFCAEQGGLVCTSSNAHKAFEYAFERGDKVLFLPDKHLGENTAHRLGMADSTAEWDPWDPESKDTEAVADADIVLWDGYCQVHERFTEDHIETIREEHDDVQVVVHPECRREVVEAADIAGSTSTICETIENADPGETWAIGTEIHLTNHLQRWHPEVNVLPLCGDACMDCNAMRQIDPNYLTWVLEELIEGRERNVIEVEPREKELAELALERMLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW73</accession>
    <name>A0A1U7EW73_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2464A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW73"/>
    <dbReference type="STRING" id="348780.NP_2464A"/>
    <dbReference type="EnsemblBacteria" id="CAI49323">
        <property type="protein sequence ID" value="CAI49323"/>
        <property type="gene ID" value="NP_2464A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_2464A"/>
    <dbReference type="eggNOG" id="arCOG02079">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028008_1_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35902:SF3">
        <property type="entry name" value="NPCBM-ASSOCIATED, NEW3 DOMAIN OF ALPHA-GALACTOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35902">
        <property type="entry name" value="S-LAYER DOMAIN-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="507"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="529" mass="57682" checksum="5B8192CA620FDF36" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW81</accession>
    <name>A0A1U7EW81_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lrp/AsnC family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2474A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW81"/>
    <dbReference type="STRING" id="348780.NP_2474A"/>
    <dbReference type="EnsemblBacteria" id="CAI49328">
        <property type="protein sequence ID" value="CAI49328"/>
        <property type="gene ID" value="NP_2474A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701456"/>
    <dbReference type="KEGG" id="nph:NP_2474A"/>
    <dbReference type="eggNOG" id="arCOG01117">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170329_1_2_2"/>
    <dbReference type="OrthoDB" id="8136at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator AsnC/Lrp ligand binding" evidence="1">
        <location>
            <begin position="6"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="76" mass="8234" checksum="2C37A3A8582465EC" modified="2017-05-10" version="1">MARSFIMVKADTGRARALLERFSDLDYIADANVVAGDIDIVLEAEADEVADIIDSVATNVRAIEGVVDTKTYVCLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWC2</accession>
    <name>A0A1U7EWC2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2582A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWC2"/>
    <dbReference type="STRING" id="348780.NP_2582A"/>
    <dbReference type="EnsemblBacteria" id="CAI49382">
        <property type="protein sequence ID" value="CAI49382"/>
        <property type="gene ID" value="NP_2582A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_2582A"/>
    <dbReference type="eggNOG" id="arCOG01497">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1202648_0_0_2"/>
    <dbReference type="OrthoDB" id="23466at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="230" mass="25927" checksum="91D928071EF11EFD" modified="2017-05-10" version="1">MPASTVVVPNIAVWWGPFGDMDREDERKPYFGEGYVEMNPNDAREEGFEDGDYVWVDADPDDRPYIGADGDPDEYARALMRVRYQPAMPENITRSWFNLNQATHGTTEATPDREGLAKNEETDYVSLYRRGGHQSTTRTWLRPTLLTDEMNRKNLMGQTIGQGFEPDVHCANGAPRESFVKFEKEGDAGEDGEGLWRPAELGLRPGYEDLGEDTDLRRYISGGYAETGGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWD7</accession>
    <name>A0A1U7EWD7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">IclR family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">NP_2618A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323025.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWD7"/>
    <dbReference type="EnsemblBacteria" id="CAI49400">
        <property type="protein sequence ID" value="CAI49400"/>
        <property type="gene ID" value="NP_2618A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701507"/>
    <dbReference type="KEGG" id="nph:NP_2618A"/>
    <dbReference type="eggNOG" id="arCOG02798">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062618_6_1_2"/>
    <dbReference type="OrthoDB" id="14763at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050707">
        <property type="entry name" value="HTH_MetabolicPath_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014757">
        <property type="entry name" value="Tscrpt_reg_IclR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005471">
        <property type="entry name" value="Tscrpt_reg_IclR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30136">
        <property type="entry name" value="HELIX-TURN-HELIX TRANSCRIPTIONAL REGULATOR, ICLR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30136:SF24">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REPRESSOR ALLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09339">
        <property type="entry name" value="HTH_IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01614">
        <property type="entry name" value="IclR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00346">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51077">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51078">
        <property type="entry name" value="ICLR_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH iclR-type" evidence="4">
        <location>
            <begin position="11"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="IclR-ED" evidence="5">
        <location>
            <begin position="71"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI49400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="252" mass="28604" checksum="4CF1929F0CFC6125" modified="2017-05-10" version="1">MDDDSTDDVGVTTTRKTFEILEVLRDEEGLSIAEITRRTSLTKSTVYRHLKTLVDTGYAIEREGAYYIGLKHLEIAEQARNREPGYTIAQRKVFELGRETDERSVFLVEEEDEGVYLHRYDSPTSTMVGQRRPLHALATGKAILAERDGEQIDRFVAENDLERYTENTITTPEALLEEVESVREQGYATNDGEYMEGLFGVAVPVYTPDDDLLGSLGLFGPRSRFSEEYIQETVLDRLWDKAGEVRVTLAYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWF2</accession>
    <name>A0A1U7EWF2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DMT superfamily transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tp24</name>
        <name evidence="7" type="ordered locus">NP_2642A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323037.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWF2"/>
    <dbReference type="STRING" id="348780.NP_2642A"/>
    <dbReference type="EnsemblBacteria" id="CAI49412">
        <property type="protein sequence ID" value="CAI49412"/>
        <property type="gene ID" value="NP_2642A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703300"/>
    <dbReference type="KEGG" id="nph:NP_2642A"/>
    <dbReference type="eggNOG" id="arCOG00271">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033863_5_1_2"/>
    <dbReference type="OrthoDB" id="17861at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050638">
        <property type="entry name" value="AA-Vitamin_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32322:SF2">
        <property type="entry name" value="EAMA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32322">
        <property type="entry name" value="INNER MEMBRANE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="130"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="251"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="276"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="6">
        <location>
            <begin position="8"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="6">
        <location>
            <begin position="161"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="307" mass="32203" checksum="FC66EA0753CB98C9" modified="2017-05-10" version="1">MRYRNTALFVLLAAVWGSAFVAIKAGVGSPDAPGYFFDAPVLFAAVRYDIAAVLMFAYVAWATDRWRPRGRTEWLAVAVGSAFIIAGYHALLFLGQRGTTSAAAAVIVSLSPVLTTAFARALLADERLSVLGLAGMGVGFLGVVVLSQPTPADIAASRFEALVFAAALCFALGSVLTRRLDTDLPIETMEAWSMAGGALLMHALSLALGETVSGAPPTDALVALGYLAVVASAAGFLVYFDLLGRLGAIEINLVSYVVPVFAALVGVLLLGERVDTPTAAGFLLVFAGFVIIKRRAIRAEIADSYWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWF9</accession>
    <name>A0A1U7EWF9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2660A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323046.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWF9"/>
    <dbReference type="STRING" id="348780.NP_2660A"/>
    <dbReference type="EnsemblBacteria" id="CAI49421">
        <property type="protein sequence ID" value="CAI49421"/>
        <property type="gene ID" value="NP_2660A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701516"/>
    <dbReference type="KEGG" id="nph:NP_2660A"/>
    <dbReference type="eggNOG" id="arCOG14890">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1665548_0_0_2"/>
    <dbReference type="OrthoDB" id="328141at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="158" mass="17892" checksum="5B5013DD5C786F1E" modified="2017-05-10" version="1">MVDHPRLIEDAPDEWLLGVSLADNAAHNFADPSREEFHLTTRATALLVDDLEYAHTEEVADETARALLLTEGAYRPDEKANPADTIQRLEQPSGGKHPTDAELERVADYLRNAEIDERAEWITEEFIEESRLESVVSPDELQTKRNRMNSLRGIAKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWN8</accession>
    <name>A0A1U7EWN8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2854A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323143.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWN8"/>
    <dbReference type="STRING" id="348780.NP_2854A"/>
    <dbReference type="EnsemblBacteria" id="CAI49518">
        <property type="protein sequence ID" value="CAI49518"/>
        <property type="gene ID" value="NP_2854A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701573"/>
    <dbReference type="KEGG" id="nph:NP_2854A"/>
    <dbReference type="HOGENOM" id="CLU_2875180_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="63" mass="6726" checksum="533A26CBA6895F7C" modified="2017-05-10" version="1">MDDAVKIALVAAVGVIAAIIVAVPVVSDEPFHLGLYLNPTLLAWLLFGWGGVVFLYFSRLDAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWX9</accession>
    <name>A0A1U7EWX9_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">siroheme decarboxylase</fullName>
            <ecNumber evidence="4">4.1.1.111</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ahbA</name>
        <name evidence="8" type="synonym">nirDL</name>
        <name evidence="8" type="ordered locus">NP_3064A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>siroheme + 2 H(+) = 12,18-didecarboxysiroheme + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:19093"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:60052"/>
            <dbReference type="ChEBI" id="CHEBI:140497"/>
            <dbReference type="EC" id="4.1.1.111"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Porphyrin-containing compound metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Ahb/Nir family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.111" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323245.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWX9"/>
    <dbReference type="STRING" id="348780.NP_3064A"/>
    <dbReference type="EnsemblBacteria" id="CAI49623">
        <property type="protein sequence ID" value="CAI49623"/>
        <property type="gene ID" value="NP_3064A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701642"/>
    <dbReference type="KEGG" id="nph:NP_3064A"/>
    <dbReference type="eggNOG" id="arCOG01628">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049427_0_0_2"/>
    <dbReference type="OrthoDB" id="145939at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3460">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040523">
        <property type="entry name" value="AsnC_trans_reg2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050684">
        <property type="entry name" value="HTH-Siroheme_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053953">
        <property type="entry name" value="NirdL-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413:SF1">
        <property type="entry name" value="SIROHEME DECARBOXYLASE NIRL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ASNC FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17805">
        <property type="entry name" value="AsnC_trans_reg2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22451">
        <property type="entry name" value="NirdL-like_HTH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Siroheme decarboxylase NirL-like HTH" evidence="7">
        <location>
            <begin position="18"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="Siroheme decarboxylase AsnC-like ligand binding" evidence="6">
        <location>
            <begin position="77"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Siroheme decarboxylase NirL-like HTH" evidence="7">
        <location>
            <begin position="192"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Siroheme decarboxylase AsnC-like ligand binding" evidence="6">
        <location>
            <begin position="249"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF17805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF22451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="347" mass="38548" checksum="D7A755284E258239" modified="2017-05-10" version="1">MSTPATDDDWRAGIDDIDAALIDGYQSGFPVTERPFRTVADDLGITESEALTRVEDLYEQGIFRRFGAVLNPPVIGASTLAAVSAPDDRFEEVAETINGYRQVNHNYRRDHEWNMWFVVTAGSLETRNAILEEIAAETGCEVLNLPMLTDFYIDLEFPVVNGDRFARESVSETTADATRISETAAADLSPLDEAILLEIQDGLPLTETPYRDVAAAVDAPVASVCESIEALRADGCIKRIGCVVNHIVTGFDANCMVVWDVPDEDLDTRGEAVGELPYVTLCYNRPRRPDQDWPYNLFTMIHGRDPDAVDEKIDELADEALPYDHERLYSTETLKQTGARYDEILGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX52</accession>
    <name>A0A1U7EX52_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Probable replication complex protein RecJ2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">recJ2</name>
        <name evidence="1" type="ordered locus">NP_3206A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323316.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX52"/>
    <dbReference type="STRING" id="348780.NP_3206A"/>
    <dbReference type="EnsemblBacteria" id="CAI49694">
        <property type="protein sequence ID" value="CAI49694"/>
        <property type="gene ID" value="NP_3206A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701683"/>
    <dbReference type="KEGG" id="nph:NP_3206A"/>
    <dbReference type="eggNOG" id="arCOG00428">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036273_0_0_2"/>
    <dbReference type="OrthoDB" id="157374at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="357" mass="36012" checksum="D9F9DDA72AC91960" modified="2017-05-10" version="1">MSAAGHPEPARDRVAATLRDAEFVRLVATADGDALAAAGLLASGLDAAEVPYQVSLAAVPDPPATDADCTVAVGTDGGDVTVSESPLAVAAAEIVRSLAAEAVDATLALAGAYCAGYEPEGWLLEQAGLERAPGIAIPTDDSIEGLAGSTLLHAGFSGDGEAAAAAVADLETADGRSLASLAALSAVEDAPPRAANAVQRFLHPHSVDRFETLGGFADVLDAVAREHPGSGIALALGHDIEETALEAWRSHGQRAHEAVRSASSSRYDGVSVVRVDGAAPDTLGTVARLWFHYRSPEPTVVAVTDGAAAVTAEGAVESPLREATTALGGRSTARNGHGTATFDASATDFTAAIREAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXB1</accession>
    <name>A0A1U7EXB1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acetyl-CoA C-acetyltransferase</fullName>
            <ecNumber evidence="4">2.3.1.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">acaB4</name>
        <name evidence="4" type="ordered locus">NP_3438A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.9" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323430.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXB1"/>
    <dbReference type="STRING" id="348780.NP_3438A"/>
    <dbReference type="EnsemblBacteria" id="CAI49810">
        <property type="protein sequence ID" value="CAI49810"/>
        <property type="gene ID" value="NP_3438A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702462"/>
    <dbReference type="KEGG" id="nph:NP_3438A"/>
    <dbReference type="eggNOG" id="arCOG01278">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035425_4_0_2"/>
    <dbReference type="OrthoDB" id="167534at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003985">
        <property type="term" value="F:acetyl-CoA C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00829">
        <property type="entry name" value="SCP-x_thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055140">
        <property type="entry name" value="Thiolase_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004720">
        <property type="entry name" value="PRK06064.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870">
        <property type="entry name" value="ACETYL-COA C-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870:SF6">
        <property type="entry name" value="ACETYL-COA C-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22691">
        <property type="entry name" value="Thiolase_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="1" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="2">
        <location>
            <begin position="6"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="3">
        <location>
            <begin position="239"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="384" mass="39256" checksum="155F65EF30808658" modified="2017-05-10" version="1">MAEIRVAGTGITDFGEHDRTTRDLFAEAGLAALDDASVPAEDVEALYYGNFIGELAERQGHQGPLVAEAAGLDCPSTRVESACASSGAAVREGVQAIHAGADVVVVGGAERMSNMGTEGATEGLATAADDLHEVRAGVTFPGAYALMASAYFHQHGGGPEDLAHIAVKNHDNALVNDHAHLQQEITVADALDAPQVASPLGLYDACPISDGASAVVLVSDDYAEENDLSAPVAITASSQASDHLALHDRESLTETPATEVAAEAAYQEAGIGPNDLDCIEVHDCFTIAEVLALEGLGVYETGEAIGAARRGETTRDGELPVNLSGGLKAKGHPVGATGAAQIVELTRQLDGTHPRAPDDASVGLAHNAGGTVASVTVHVLEVAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXD4</accession>
    <name>A0A1U7EXD4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DUF418 domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_3452A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323437.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXD4"/>
    <dbReference type="STRING" id="348780.NP_3452A"/>
    <dbReference type="EnsemblBacteria" id="CAI49817">
        <property type="protein sequence ID" value="CAI49817"/>
        <property type="gene ID" value="NP_3452A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701777"/>
    <dbReference type="KEGG" id="nph:NP_3452A"/>
    <dbReference type="eggNOG" id="arCOG06418">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039610_0_0_2"/>
    <dbReference type="OrthoDB" id="268997at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052529">
        <property type="entry name" value="Bact_Transport_Assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007349">
        <property type="entry name" value="DUF418"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30590">
        <property type="entry name" value="INNER MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30590:SF2">
        <property type="entry name" value="INNER MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04235">
        <property type="entry name" value="DUF418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="87"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="116"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="140"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="162"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="274"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="312"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="353"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="380"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="DUF418" evidence="3">
        <location>
            <begin position="256"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="429" mass="47281" checksum="C3D06022E2EAE894" modified="2017-05-10" version="1">MPSDGRDCANDGSPTENSGESGPTAPEDRIVSLDVLRGVAILGILVINIWLFGLPMAAGMNPTLYGDFSGLNYLAWLVSHVFFEQKFVTLFTFLFGAGIVLFVESKEAAGRPARRLYFRRTGWLLVAGLLHAYLLWHGDILVAYALCGMLVVFVRNWRPRRLLLLGVAMFALPSLLYLVGGAGYMVADATTQDEINAAVTAGVGGDAIAAEIDAYRGSWTEQIQHRAPTVLMLQTFGFAVETFWMLGGLMSIGMALYKYGIISNRRASRFYRRLLVAGGTAGLALILTGVWYREAVGWETAPVVLLAHQFNYWGALLLSLSYVAAVMLFCRAAAESRAAGALAAVGRTAFSNYILQTVLATTLFYGHGVGWFGTLSRIELLGVVVAIWAIQVPLSVAWLRRYRFGPIEWLWRTLTYRQWQPLRVGEKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXF9</accession>
    <name>A0A1U7EXF9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Probable NADH dehydrogenase</fullName>
            <ecNumber evidence="7">1.6.99.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ndh</name>
        <name evidence="7" type="ordered locus">NP_3508A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NADH dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.6.99.3" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323465.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXF9"/>
    <dbReference type="STRING" id="348780.NP_3508A"/>
    <dbReference type="EnsemblBacteria" id="CAI49845">
        <property type="protein sequence ID" value="CAI49845"/>
        <property type="gene ID" value="NP_3508A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702970"/>
    <dbReference type="KEGG" id="nph:NP_3508A"/>
    <dbReference type="eggNOG" id="arCOG01067">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021377_7_2_2"/>
    <dbReference type="OrthoDB" id="6639at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003955">
        <property type="term" value="F:NAD(P)H dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019646">
        <property type="term" value="P:aerobic electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051169">
        <property type="entry name" value="NADH-Q_oxidoreductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42913:SF3">
        <property type="entry name" value="64 KDA MITOCHONDRIAL NADH DEHYDROGENASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42913">
        <property type="entry name" value="APOPTOSIS-INDUCING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="6">
        <location>
            <begin position="4"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="388" mass="41969" checksum="2325FBC495590C4B" modified="2017-05-10" version="1">MTDDVVVLGSGYAGTGAINSLESELDGEADITWISDVDHHLVLHESHRCIRDPDIQEKITFDVTEIKSPSTRFVQGRVEDIDTDERVITLDDDSTVEYDYLLVGIGSQTAFFGIDGLEEHSLTLKTLDDALEIHEAVSEAAREASRNDPAQVIVGGAGLSGIQSAGEIAEYRDSNRAPLEIHLVEGLDNVFPNNDPVVQAKLRKLLEDKDVQIHTGEFIGEVDDDTVYVGDEKELDYDVLLWTGGITGQDAAETANVDKDDRSNRIEAASNFQTSDERVFAIGDAALIDQPDQDNPAPPTAQAAWQAAEVAGENIAREIRGQPLTEWEYEDKGTLISVGGKAVAHNVKGLPVDTFGGPAAKALKKTVAARWIAKVTGFSDAAKAFPDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXG0</accession>
    <name>A0A1U7EXG0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3540A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323481.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXG0"/>
    <dbReference type="STRING" id="348780.NP_3540A"/>
    <dbReference type="EnsemblBacteria" id="CAI49861">
        <property type="protein sequence ID" value="CAI49861"/>
        <property type="gene ID" value="NP_3540A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701800"/>
    <dbReference type="KEGG" id="nph:NP_3540A"/>
    <dbReference type="eggNOG" id="arCOG04533">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1127063_0_0_2"/>
    <dbReference type="OrthoDB" id="236676at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="255" mass="27481" checksum="34DBA752E8299CE8" modified="2017-05-10" version="1">MSTQDGGHRDAAIAALAERAYVDAGDEYTRAGRRVLADPRPDGGPFELGDHGWVGAGVQYLLIAALCYRVADEPQTARFRAVEATAVAGDFARRADSPAQSACFAELRADCRVAGGLSEASEAYNDAVDAYEAAGETVDDPSRMSTTPLFQAAAAPIKQAARSLANGEIAVEWDDLHGPDPSDPGRFLAVRAEYKRRRFPGLVERLLSERYLAAPRGTTEYATDHHRCPHCDSTDVNWVGNSTLCLRCSRPTNPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXL4</accession>
    <name>A0A1U7EXL4_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">tRNA(Met) cytidine acetyltransferase TmcA</fullName>
            <ecNumber evidence="12">2.3.1.193</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12 17" type="primary">tmcA</name>
        <name evidence="17" type="ordered locus">NP_3690A</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the formation of N(4)-acetylcytidine (ac(4)C) at the wobble position of tRNA(Met), by using acetyl-CoA as an acetyl donor and ATP (or GTP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a cytidine in RNA + acetyl-CoA + ATP + H2O = an N(4)-acetylcytidine in RNA + ADP + phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:82211"/>
            <dbReference type="Rhea" id="RHEA-COMP:15704"/>
            <dbReference type="Rhea" id="RHEA-COMP:19834"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:74900"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a cytidine in mRNA + acetyl-CoA + ATP + H2O = an N(4)-acetylcytidine in mRNA + ADP + phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:58480"/>
            <dbReference type="Rhea" id="RHEA-COMP:15145"/>
            <dbReference type="Rhea" id="RHEA-COMP:15146"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:74900"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a cytidine in tRNA + acetyl-CoA + ATP + H2O = an N(4)-acetylcytidine in tRNA + ADP + phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53876"/>
            <dbReference type="Rhea" id="RHEA-COMP:13670"/>
            <dbReference type="Rhea" id="RHEA-COMP:13671"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:74900"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>cytidine(34) in elongator tRNA(Met) + acetyl-CoA + ATP + H2O = N(4)-acetylcytidine(34) in elongator tRNA(Met) + ADP + phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43788"/>
            <dbReference type="Rhea" id="RHEA-COMP:10693"/>
            <dbReference type="Rhea" id="RHEA-COMP:10694"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:74900"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.3.1.193"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the TmcA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.193" evidence="12"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323554.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXL4"/>
    <dbReference type="STRING" id="348780.NP_3690A"/>
    <dbReference type="EnsemblBacteria" id="CAI49936">
        <property type="protein sequence ID" value="CAI49936"/>
        <property type="gene ID" value="NP_3690A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701840"/>
    <dbReference type="KEGG" id="nph:NP_3690A"/>
    <dbReference type="eggNOG" id="arCOG01951">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004652_1_0_2"/>
    <dbReference type="OrthoDB" id="312894at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990883">
        <property type="term" value="F:18S rRNA cytidine N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106162">
        <property type="term" value="F:mRNA N-acetyltransferase activity"/>
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        <property type="project" value="RHEA"/>
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        <property type="term" value="F:tRNA binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051392">
        <property type="term" value="F:tRNA cytidine N4-acetyltransferase activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904812">
        <property type="term" value="P:rRNA acetylation involved in maturation of SSU-rRNA"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:tRNA acetylation"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002101">
        <property type="term" value="P:tRNA wobble cytosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="tRNA(Met) cytidine acetyltransferase, tail domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="tRNA_acetyltr_TmcA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
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        <property type="entry name" value="TcmA/NAT10_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032672">
        <property type="entry name" value="TmcA/NAT10/Kre33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013562">
        <property type="entry name" value="TmcA/NAT10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038321">
        <property type="entry name" value="TmcA_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024914">
        <property type="entry name" value="tRNA_acetyltr_TmcA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053477">
        <property type="entry name" value="tRNA_Cytidine_AcTrnsfr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041296">
        <property type="entry name" value="RNAactase_tcmA_Halo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10925">
        <property type="entry name" value="N-ACETYLTRANSFERASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10925:SF5">
        <property type="entry name" value="RNA CYTIDINE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13718">
        <property type="entry name" value="GNAT_acetyltr_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05127">
        <property type="entry name" value="NAT10_TcmA_helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08351">
        <property type="entry name" value="TmcA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 17" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 12 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 12" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="2 12" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="TmcA/NAT10 N-terminal" evidence="15">
        <location>
            <begin position="4"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="TcmA/NAT10 helicase" evidence="14">
        <location>
            <begin position="222"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="16">
        <location>
            <begin position="433"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="16">
        <location>
            <begin position="551"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="376"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="522"/>
            <end position="524"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00049889"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00049914"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="HAMAP-Rule" id="MF_01886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF05127"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF08351"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF13718"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI49936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="757" mass="80650" checksum="E90CD1E22AE7A332" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXN5</accession>
    <name>A0A1U7EXN5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cupin 2 barrel domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3760A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323588.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
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    <dbReference type="STRING" id="348780.NP_3760A"/>
    <dbReference type="EnsemblBacteria" id="CAI49971">
        <property type="protein sequence ID" value="CAI49971"/>
        <property type="gene ID" value="NP_3760A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701858"/>
    <dbReference type="KEGG" id="nph:NP_3760A"/>
    <dbReference type="eggNOG" id="arCOG02998">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1870798_0_0_2"/>
    <dbReference type="OrthoDB" id="82049at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="cupin_RmlC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051610">
        <property type="entry name" value="GPI/OXD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35848">
        <property type="entry name" value="OXALATE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin type-2" evidence="2">
        <location>
            <begin position="44"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="132" mass="14607" checksum="D44E87ED5BC92117" modified="2017-05-10" version="1">MDYEVVDLDDVPTTDLSEIDEVPPDLDIKPVGEQLGLSELRATVWYFEPGDEIQYHAHHEQEEVYFVLDGEFSLKLGRSGETEYVEVDSGTFWVAKPEVGHGHRYIGDDEGVVLSLGAPPVADPGVDPHGLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXS6</accession>
    <name>A0A1U7EXS6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cobalamin import ATP-binding protein BtuD</fullName>
            <ecNumber evidence="8">7.6.2.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Vitamin B12-transporting ATPase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">btuD</name>
        <name evidence="13" type="synonym">abc16a:fhuC</name>
        <name evidence="13" type="ordered locus">NP_3818A</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for corrinoid utilization. Probably part of the ABC transporter complex BtuCDF involved in cobalamin (vitamin B12) import. Probably responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>an R-cob(III)alamin(out) + ATP + H2O = an R-cob(III)alamin(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17873"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:140785"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.6.2.8"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">The complex is composed of two ATP-binding proteins (BtuD), two transmembrane proteins (BtuC) and a solute-binding protein (BtuF).</text>
    </comment>
    <dbReference type="EC" id="7.6.2.8" evidence="8"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323617.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXS6"/>
    <dbReference type="STRING" id="348780.NP_3818A"/>
    <dbReference type="EnsemblBacteria" id="CAI50000">
        <property type="protein sequence ID" value="CAI50000"/>
        <property type="gene ID" value="NP_3818A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702417"/>
    <dbReference type="KEGG" id="nph:NP_3818A"/>
    <dbReference type="eggNOG" id="arCOG00199">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_0_0_2"/>
    <dbReference type="OrthoDB" id="24644at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015420">
        <property type="term" value="F:ABC-type vitamin B12 transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03214">
        <property type="entry name" value="ABC_Iron-Siderophores_B12_Hemin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000134">
        <property type="entry name" value="Iron-enterobactin ABC transporter ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007082">
        <property type="entry name" value="PRK09536.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010068">
        <property type="entry name" value="PRK13548.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42794">
        <property type="entry name" value="HEMIN IMPORT ATP-BINDING PROTEIN HMUV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42794:SF1">
        <property type="entry name" value="HEMIN IMPORT ATP-BINDING PROTEIN HMUV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-1278">Translocase</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="2"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="360"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00050590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00064420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00077139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAI50000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="394" mass="40435" checksum="DE28F3035A39F854" modified="2017-05-10" version="1">MISLSDVVVEFGETTVLDGVSLSVEEGEFLALVGPNGAGKTTLLRTCNGVLTPAAGTVTVAGRDVAATSARELGRQVATVPQETTLAFEFDVEAVVRMGRTPHRSRLSPADSAGRAAVESALERTETAAFADRSIDSLSGGERQRVMLARALAQATPVLLLDEPTASLDINHQVRTLALARELADDGKTVVAAIHDLELAARFCDTVALLSDGRILDSGRPEAVLTAERLEAAFGVRTAVGTNPVTGSPSVTPLADAPPDGQRVHVLGAGQPAARLLGDLAERGIDVTAGPLPTGDVAAATAGSVGREAVTAPPFKPVSESDIEAATDLIEAADVTVLAGRPAGPTERLTARSERLLALPGTDAPPSAHIAAPGDIVDALEDPTRRQTSSSAVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXU0</accession>
    <name>A0A1U7EXU0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3826A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXU0"/>
    <dbReference type="STRING" id="348780.NP_3826A"/>
    <dbReference type="EnsemblBacteria" id="CAI50004">
        <property type="protein sequence ID" value="CAI50004"/>
        <property type="gene ID" value="NP_3826A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3826A"/>
    <dbReference type="HOGENOM" id="CLU_3148067_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="48" mass="5100" checksum="E2750F252426642E" modified="2017-05-10" version="1">MHMGLCSTFRSLVVYGLAIVGLAAVLFVVLAYGWDGEPPEVSLESDGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXV2</accession>
    <name>A0A1U7EXV2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF2391 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3904A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323660.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXV2"/>
    <dbReference type="EnsemblBacteria" id="CAI50043">
        <property type="protein sequence ID" value="CAI50043"/>
        <property type="gene ID" value="NP_3904A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701912"/>
    <dbReference type="KEGG" id="nph:NP_3904A"/>
    <dbReference type="eggNOG" id="arCOG03887">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098904_0_0_2"/>
    <dbReference type="OrthoDB" id="328136at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="229" mass="25250" checksum="29858B7D7C1D5F67" modified="2017-05-10" version="1">MSRDEDNTTDPPVDGQPESQESLPNGLDGSTAEYDIDDVYKQLQLIEETVTSDAEREEVRRARKMLKRVPGSDHINKYTTRDMGESFVGGVVFSLPLLVEDGVFEIAEWFAEHTIAQVPIFLVLNVLFIGVLVTGLLYAIDVREITITKPIFGFIPRRLVGVLAISLIVAVGSMFMWGRLHEEDPATIEQFGRVTVIWAAAALGAVLADILPGESKGEDISEMIDGDDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXW4</accession>
    <name>A0A1U7EXW4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3942A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323679.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXW4"/>
    <dbReference type="STRING" id="348780.NP_3942A"/>
    <dbReference type="EnsemblBacteria" id="CAI50062">
        <property type="protein sequence ID" value="CAI50062"/>
        <property type="gene ID" value="NP_3942A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701918"/>
    <dbReference type="KEGG" id="nph:NP_3942A"/>
    <dbReference type="HOGENOM" id="CLU_1399780_0_0_2"/>
    <dbReference type="OrthoDB" id="385385at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="194" mass="22129" checksum="E2217476CBBF79C6" modified="2017-05-10" version="1">MTTPAAHFTDEEIQAVINHYDDPEHPDALTISDARELLATLQETLEDEWDEYTTAIREDTLSVARDTGSLVVFEDPERTRWTQLLDAVQLYNQVERTILRVIHHQAAKRLTDRDFDGTDPLVVRKPQSALAGQRLVEAVVNALWADGLTADEAWTYYAIDLRGYDADEWLERGGYEDRITVADTVERARDKLGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXW8</accession>
    <name>A0A1U7EXW8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF91 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3884A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323650.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXW8"/>
    <dbReference type="EnsemblBacteria" id="CAI50033">
        <property type="protein sequence ID" value="CAI50033"/>
        <property type="gene ID" value="NP_3884A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701902"/>
    <dbReference type="KEGG" id="nph:NP_3884A"/>
    <dbReference type="eggNOG" id="arCOG07683">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_800789_0_0_2"/>
    <dbReference type="OrthoDB" id="191938at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="346" mass="39622" checksum="FF6AFC17506ABA3C" modified="2017-05-10" version="1">MLRISEDEKTTSLEPTSLSDQGYKEEDLREWILDNPHSILGEDILIIGREVKVQYIGDAIDLLGIDRDGNVVVIELKTGSLRGNVDFQSLKYAAYTSYWDWDHLSAQFDKFTDSKWGQKLYNGEVDFNEKLESFCNDEYELNQNQRILLVGESIRERLDLVVRWLSERDVEISVLEVDMLQDGNQLYLDTEQTIPIPEDTVSEISPQTSKEPWKVDGKSWHLDKKLNDNTADRLREVVEALGNVESLDGPHWGQKQYVSFKQNRKNRVIARTRTEVFKVEIYDIPADDIDVEELADSVGVAKDDVRAYADDLRGGRSGILVTLGGDRDIDAEQLAEKVDEYLAKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXY8</accession>
    <name>A0A1U7EXY8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Chorismate mutase</fullName>
            <ecNumber evidence="3">5.4.99.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">aroQ</name>
        <name evidence="3" type="ordered locus">NP_3980A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
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    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50081.3"/>
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    <dbReference type="EnsemblBacteria" id="CAI50081">
        <property type="protein sequence ID" value="CAI50081"/>
        <property type="gene ID" value="NP_3980A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3980A"/>
    <dbReference type="eggNOG" id="arCOG02098">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131518_4_1_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004106">
        <property type="term" value="F:chorismate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="P:chorismate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009697">
        <property type="term" value="P:salicylic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Chorismate mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Chorismate_II_sf"/>
    </dbReference>
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        <property type="entry name" value="Chorismate_mutase-related"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CM_II_prokaryot"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01791">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chorismate mutase" evidence="2">
        <location>
            <begin position="5"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50081.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="96" mass="10990" checksum="A19018F2371BD5E6" modified="2017-05-10" version="1">MTRGDPAEMDLDELRKEIQSIDQEIVELIAQRTYVAESIAAVKRERGLPTTDESQEEAVMERAGENAEQFDVDANLVKAIFRLLIELNKVEQRESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXZ1</accession>
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        <submittedName>
            <fullName evidence="2">Rpa-associated protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="primary">rpap1</name>
        <name evidence="2" type="ordered locus">NP_3962A</name>
    </gene>
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        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <lineage>
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            <taxon>Natronomonas</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50072.1"/>
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    </dbReference>
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    <dbReference type="STRING" id="348780.NP_3962A"/>
    <dbReference type="EnsemblBacteria" id="CAI50072">
        <property type="protein sequence ID" value="CAI50072"/>
        <property type="gene ID" value="NP_3962A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701923"/>
    <dbReference type="KEGG" id="nph:NP_3962A"/>
    <dbReference type="eggNOG" id="arCOG02258">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_489691_0_0_2"/>
    <dbReference type="OrthoDB" id="214631at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="260"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="325"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="351"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="379"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="419"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="505"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="587" mass="60748" checksum="13FF684D04E3F4DC" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY00</accession>
    <name>A0A1U7EY00_NATPD</name>
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        <submittedName>
            <fullName evidence="9">Uracil-DNA glycosylase superfamily protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">NP_3952A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
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            <taxon>Stenosarchaea group</taxon>
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            <taxon>Natronomonas</taxon>
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    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:deaminated base DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Uracil-DNA glycosylase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
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        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33693:SF1">
        <property type="entry name" value="TYPE-4 URACIL-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TYPE-5 URACIL-DNA GLYCOSYLASE"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uracil-DNA glycosylase-like" evidence="8">
        <location>
            <begin position="24"/>
            <end position="163"/>
        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI50067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="195" mass="20817" checksum="19E12F881CFAC6EB" modified="2017-05-10" version="1">MERVTDRVSNPFGMSAPDEPAVYGYGDANADFHVLGADAERHGGRETGVPFTGSLAGVRLQRVLHATGFAAAAYSDAPTLTNCYLSYLCLSPGPAAELERYADAELRALNAHILLPVGDDPLEYVLEAYTTQARKLPADAAKLHAEEIRGRGFLVVPVADPATWTDAEEAALVERLTALLNSDYRQTKGVATTVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY12</accession>
    <name>A0A1U7EY12_NATPD</name>
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        <submittedName>
            <fullName evidence="4">Probable secreted glycoprotein</fullName>
        </submittedName>
    </protein>
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        <name evidence="4" type="ordered locus">NP_4008A</name>
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    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
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        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Halobacteria</taxon>
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            <taxon>Natronomonadaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50095.1"/>
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        <property type="protein sequence ID" value="CAI50095"/>
        <property type="gene ID" value="NP_4008A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701937"/>
    <dbReference type="KEGG" id="nph:NP_4008A"/>
    <dbReference type="eggNOG" id="arCOG02911">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029417_0_0_2"/>
    <dbReference type="OrthoDB" id="148042at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055713">
        <property type="entry name" value="DUF7289"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055729">
        <property type="entry name" value="DUF7305"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23960">
        <property type="entry name" value="DUF7289"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23981">
        <property type="entry name" value="DUF7305"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7305" evidence="3">
        <location>
            <begin position="260"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="472" mass="49922" checksum="572A1B2B9055DF49" modified="2017-05-10" version="1">MDDRAVSNTVGIVLILGMTIAAVSALVFVGGAVLEDTRADTEQSQVESSMSQFSSKASLVGLGESDAQEFALGRITDGQVTVEPDEGHVLMYVEDAEGDRDDIGDVSLGAMVYENGDTEIAYQGGGVWERTGDHSRMVSPPEFHYRSETLTFPILNVSGAGSASGDVRGAATRGDDPRQLYPNVSKNEDFDNPLTDGTVYIEVESRYCEGWESFFRDRSQGGLDETCDEGDSDTLVVDLTASFEPAFGATVTAKQIENDGAEVDGSTREGIIAPSASDRIEQRIDDCETNGCRDDFSSTLTHGTYYTTDADDIENIDIDTTDGAVDIVVDDSDGVDGTGSIDIVDGDATVSLYVGPGGEFSMTGGSDEVNVGGEPSQFITYIHSDVPAVRMNSATYVGGIYAPNTVMEGDQGGGGGCGGGSVDVTGSVVVENFCFQNGAFTHDDDMSDIDVDIDANTVKYLHVSENRVTIDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY56</accession>
    <name>A0A1U7EY56_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aminopyrimidine aminohydrolase</fullName>
            <ecNumber evidence="2">3.5.99.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">tenA2</name>
        <name evidence="2" type="ordered locus">NP_4082A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.99.2" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323748.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY56"/>
    <dbReference type="STRING" id="348780.NP_4082A"/>
    <dbReference type="EnsemblBacteria" id="CAI50132">
        <property type="protein sequence ID" value="CAI50132"/>
        <property type="gene ID" value="NP_4082A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701964"/>
    <dbReference type="KEGG" id="nph:NP_4082A"/>
    <dbReference type="eggNOG" id="arCOG01128">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077537_3_0_2"/>
    <dbReference type="OrthoDB" id="85443at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050334">
        <property type="term" value="F:thiaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006772">
        <property type="term" value="P:thiamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19366">
        <property type="entry name" value="TenA_C_BhTenA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.910.10">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016084">
        <property type="entry name" value="Haem_Oase-like_multi-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004305">
        <property type="entry name" value="Thiaminase-2/PQQC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027574">
        <property type="entry name" value="Thiaminase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050967">
        <property type="entry name" value="Thiamine_Salvage_TenA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04306">
        <property type="entry name" value="salvage_TenA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43198">
        <property type="entry name" value="BIFUNCTIONAL TH2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43198:SF2">
        <property type="entry name" value="SI:CH1073-67J19.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03070">
        <property type="entry name" value="TENA_THI-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48613">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiaminase-2/PQQC" evidence="1">
        <location>
            <begin position="13"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="219" mass="25577" checksum="C1706B2193DF48BE" modified="2017-05-10" version="1">MDFSDELLEEGSHIWDAQYEHPFVTELAEGTLDPEAFQHWVKQDYRYLLDYARTFALAGTKARREETMAGLFDVAHTVLNYEMDLHREFAAEYGIDPEELEAVQKAPTCIGYTNFLVRTAYEGTLPEIMAAIYPCGQGYLDVAEHMQERATEEHRYTPFIEKYTSDEFYDAVDQMRTLVNRCAEENPGEREAMREAFLTSARLEHQFWEMCYTQEGWSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY74</accession>
    <name>A0A1U7EY74_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">GNAT family acetyltransferase</fullName>
            <ecNumber evidence="4">2.3.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_4128A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323771.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY74"/>
    <dbReference type="STRING" id="348780.NP_4128A"/>
    <dbReference type="EnsemblBacteria" id="CAI50155">
        <property type="protein sequence ID" value="CAI50155"/>
        <property type="gene ID" value="NP_4128A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701983"/>
    <dbReference type="KEGG" id="nph:NP_4128A"/>
    <dbReference type="eggNOG" id="arCOG00826">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105299_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050832">
        <property type="entry name" value="Bact_Acetyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43877">
        <property type="entry name" value="AMINOALKYLPHOSPHONATE N-ACETYLTRANSFERASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="9"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="168" mass="18302" checksum="8A91F6775EA9A55E" modified="2017-05-10" version="1">MSGEPPVDVDIEPADADDADRIADMWVDLATDQRRHGSHLRPEENRSRIHESMLQHAVADTALVARRAGAVVGFVTYGVETDAYEQDVTRGIVYNLYVTPDHRGEGIGSALLAAAEAELDADGADAVGLQAMADNEAARSFYRDHGYTPHRVELEKAIRTDTLKTDDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY75</accession>
    <name>A0A1U7EY75_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Alpha/beta hydrolase fold protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_4164A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. FUS2 hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323789.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY75"/>
    <dbReference type="STRING" id="348780.NP_4164A"/>
    <dbReference type="ESTHER" id="natpd-q3inx9">
        <property type="family name" value="LipSM54-like_FamXVI"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI50173">
        <property type="protein sequence ID" value="CAI50173"/>
        <property type="gene ID" value="NP_4164A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701990"/>
    <dbReference type="KEGG" id="nph:NP_4164A"/>
    <dbReference type="eggNOG" id="arCOG01646">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022401_1_0_2"/>
    <dbReference type="OrthoDB" id="25019at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005674">
        <property type="entry name" value="CocE/Ser_esterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050261">
        <property type="entry name" value="FrsA_esterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000383">
        <property type="entry name" value="Xaa-Pro-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013736">
        <property type="entry name" value="Xaa-Pro_dipept_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00976">
        <property type="entry name" value="CocE_NonD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22946">
        <property type="entry name" value="DIENELACTONE HYDROLASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22946:SF9">
        <property type="entry name" value="POLYKETIDE TRANSFERASE AF380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02129">
        <property type="entry name" value="Peptidase_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08530">
        <property type="entry name" value="PepX_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00939">
        <property type="entry name" value="PepX_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xaa-Pro dipeptidyl-peptidase C-terminal" evidence="3">
        <location>
            <begin position="325"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="507" mass="54257" checksum="7C79032B3064BC3E" modified="2017-05-10" version="1">MGVTRRTVLRTLAAGGVAAAGTTTAVGQASAIELDCALSGELFEPSGDVTSRDVTIASFDGTELAATVYEPDTAGPHPSVLTTHGWGLNKDLMRCTAQMYASHGYVVLAYDSRGFGDSDGEVQVNGPNEVRDVSALLDWLADYDEVRADGDNPAVGMDGGSYGGGIQLLAAAQDDRIDAIVPRIAWNDLVYSGAPNETIKSGWLGALVGSGAVTSRFTGNIEQTLDERLPEWYGEALEANALPADAESYFEARSPATYLDDLDAPTLVVSGWKDTLFPPSEAVANYRQASAVGVDARLVIYNGGHNIAELGVGDDQQTYMHEAALEWMNAHVRGDGDPDIPEVSLYDEQQETWRTFEGFGDGAEPVAVSLDSLADDRSTVRQAPLWASRTADYDIGSDGAFEVFGTPELTLTVETDNDVLNLFVSFSHVDRWGRSEQIDDQVTAVRIEDGTQTVALELEPLQRSVPDGETLRLEIAAEDPFYLDEYGSATVHNDGSTLELPLADGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY79</accession>
    <name>A0A1U7EY79_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Serine acetyltransferase</fullName>
            <ecNumber evidence="4">2.3.1.30</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">cysE</name>
        <name evidence="14" type="ordered locus">NP_4172A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-serine + acetyl-CoA = O-acetyl-L-serine + CoA</text>
            <dbReference type="Rhea" id="RHEA:24560"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:58340"/>
            <dbReference type="EC" id="2.3.1.30"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.30" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323793.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY79"/>
    <dbReference type="STRING" id="348780.NP_4172A"/>
    <dbReference type="EnsemblBacteria" id="CAI50177">
        <property type="protein sequence ID" value="CAI50177"/>
        <property type="gene ID" value="NP_4172A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702651"/>
    <dbReference type="KEGG" id="nph:NP_4172A"/>
    <dbReference type="eggNOG" id="arCOG01847">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051638_10_1_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009001">
        <property type="term" value="F:serine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03354">
        <property type="entry name" value="LbH_SAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3130.10:FF:000003">
        <property type="entry name" value="Serine acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.10.10:FF:000007">
        <property type="entry name" value="Serine acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3130.10">
        <property type="entry name" value="serine acetyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045304">
        <property type="entry name" value="LbH_SAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042122">
        <property type="entry name" value="Ser_AcTrfase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005881">
        <property type="entry name" value="Ser_O-AcTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053376">
        <property type="entry name" value="Serine_acetyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01172">
        <property type="entry name" value="cysE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041874">
        <property type="entry name" value="EPS_EpsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42811">
        <property type="entry name" value="SERINE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000441">
        <property type="entry name" value="CysE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 14" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="166"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAI50177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="189" mass="19746" checksum="65878F092D7448F3" modified="2017-05-10" version="1">MSLHNLIRTIRRHLREDIRTALAKDPAATSALSVALLYPGLHAVWGYRIAHALWERGHTFAARALSQCIRLLTGVEIHPAADIGRRLFIDHGAAVVVGETAEIGDDVLLYHGVTLGGDSMRREKRHPTLADGATVGANATLIGDITVGESATVGAGSVVVEDVAPGQTVAGSPAEPVDGTGSERVDGPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYA0</accession>
    <name>A0A1U7EYA0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">(S)-8-amino-7-oxononanoate synthase BioU</fullName>
            <ecNumber evidence="1">2.6.1.121</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">8-amino-7-oxononanoate carboxylating dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">bioU</name>
        <name evidence="2" type="ordered locus">NP_4234A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">A 'suicide' enzyme that participates in biotin synthesis. Catalyzes the formation of (S)-8-amino-7-oxononanoate (DAN-carbamic acid) from (7R,8S)-8-amino-7-(carboxyamino)nonanoate (DAN), a function equivalent to the cannonical BioA reaction and the first half-reaction of BioD. The cellular requirement for biotin is thought be low enough that this single turnover enzyme supplies a sufficient amount of the cofactor. Overall it catalyzes three reactions: formation of a covalent linkage with 8-amino-7-oxononanoate to yield a BioU-DAN conjugate at the epsilon-amino group of Lys124 of BioU using NAD(P)H, carboxylation of the conjugate to form BioU-DAN-carbamic acid, and release of DAN-carbamic acid using NAD(P)+.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(8S)-8-amino-7-oxononanoate + L-lysyl-[protein] + CO2 = (7R,8S)-8-amino-7-(carboxyamino)nonanoate + (S)-2-amino-6-oxohexanoyl-[protein] + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:63660"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:12448"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:131803"/>
            <dbReference type="ChEBI" id="CHEBI:149468"/>
            <dbReference type="ChEBI" id="CHEBI:149470"/>
            <dbReference type="EC" id="2.6.1.121"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(8S)-8-amino-7-oxononanoate + L-lysyl-[protein] + NADH + H(+) = N(6)-[(2S,3R)-2-amino-8-carboxyoctan-3-yl]-L-lysyl-[protein] + NAD(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:63672"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:16405"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:149468"/>
            <dbReference type="ChEBI" id="CHEBI:149472"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(8S)-8-amino-7-oxononanoate + L-lysyl-[protein] + NADPH + H(+) = N(6)-[(2S,3R)-2-amino-8-carboxyoctan-3-yl]-L-lysyl-[protein] + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:63664"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:16405"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:149468"/>
            <dbReference type="ChEBI" id="CHEBI:149472"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N(6)-[(2S,3R)-2-amino-8-carboxyoctan-3-yl]-L-lysyl-[protein] + CO2 + NAD(+) + H2O = (7R,8S)-8-amino-7-(carboxyamino)nonanoate + (S)-2-amino-6-oxohexanoyl-[protein] + NADH + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:63676"/>
            <dbReference type="Rhea" id="RHEA-COMP:12448"/>
            <dbReference type="Rhea" id="RHEA-COMP:16405"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:131803"/>
            <dbReference type="ChEBI" id="CHEBI:149470"/>
            <dbReference type="ChEBI" id="CHEBI:149472"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N(6)-[(2S,3R)-2-amino-8-carboxyoctan-3-yl]-L-lysyl-[protein] + CO2 + NADP(+) + H2O = (7R,8S)-8-amino-7-(carboxyamino)nonanoate + (S)-2-amino-6-oxohexanoyl-[protein] + NADPH + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:63668"/>
            <dbReference type="Rhea" id="RHEA-COMP:12448"/>
            <dbReference type="Rhea" id="RHEA-COMP:16405"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:131803"/>
            <dbReference type="ChEBI" id="CHEBI:149470"/>
            <dbReference type="ChEBI" id="CHEBI:149472"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; biotin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">In cannonical biotin synthesis a pimeloyl-conjugate is transformed into biotin by the subsequent action of BioF, BioA, BioD and BioB. This enzyme replaces BioA and performs the first half-reaction of BioD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the BioU family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.121" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323824.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYA0"/>
    <dbReference type="STRING" id="348780.NP_4234A"/>
    <dbReference type="EnsemblBacteria" id="CAI50208">
        <property type="protein sequence ID" value="CAI50208"/>
        <property type="gene ID" value="NP_4234A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702009"/>
    <dbReference type="KEGG" id="nph:NP_4234A"/>
    <dbReference type="eggNOG" id="arCOG06241">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072022_0_0_2"/>
    <dbReference type="OrthoDB" id="146750at2157"/>
    <dbReference type="UniPathway" id="UPA00078"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00852">
        <property type="entry name" value="BioU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044262">
        <property type="entry name" value="BioU-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="1" id="KW-0093">Biotin biosynthesis</keyword>
    <keyword evidence="1" id="KW-0520">NAD</keyword>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor and proton acceptor" evidence="1">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="10"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="203"/>
            <end position="204"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Allysine" evidence="1">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="342" mass="36473" checksum="9434F126FB8786A9" modified="2017-05-10" version="1">MDDINFAVLGTGGIGRRTLEYATEKTALTPVAACDRHGVAIDHDGLDVDELLAATEGNIASDGGAVAVKQSGEMRGVAASTQAEPTETPIDDIIAESDAIDAVLIALPNLEHDFIPRVADRFADADYEGVLVDVLKRSRVIGMLDDRVDSFEDAGITFVCGAGATPGFLTGAAALAAQSFVEVESVDIWWGVGLKSGYEDNRGTVREDIAHLDGYDIETARELSDEEIEAIVDEHDGRIEFNDMEHADDVLLERAGICDAEDVTVGGILDVRSDEKPTTTTVEVTGRTFDGERATNTFQLDDDTSMEANVNGPALGYLKAGVRRNRAGEYGVFGPTDLMPGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYD1</accession>
    <name>A0A1U7EYD1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4282A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323848.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYD1"/>
    <dbReference type="STRING" id="348780.NP_4282A"/>
    <dbReference type="EnsemblBacteria" id="CAI50232">
        <property type="protein sequence ID" value="CAI50232"/>
        <property type="gene ID" value="NP_4282A"/>
    </dbReference>
    <dbReference type="GeneID" id="43500096"/>
    <dbReference type="KEGG" id="nph:NP_4282A"/>
    <dbReference type="eggNOG" id="arCOG09387">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_216660_0_0_2"/>
    <dbReference type="OrthoDB" id="275120at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="47" mass="5190" checksum="F4ABB3159CC5A63A" modified="2017-05-10" version="1">MRPNIDISHTLGGRVKDYAEANDLDLSEAYTEVLEAGLEALETQDQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYD6</accession>
    <name>A0A1U7EYD6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">AstE domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">NP_4272A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323843.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYD6"/>
    <dbReference type="SMR" id="A0A1U7EYD6"/>
    <dbReference type="STRING" id="348780.NP_4272A"/>
    <dbReference type="EnsemblBacteria" id="CAI50227">
        <property type="protein sequence ID" value="CAI50227"/>
        <property type="gene ID" value="NP_4272A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702015"/>
    <dbReference type="KEGG" id="nph:NP_4272A"/>
    <dbReference type="eggNOG" id="arCOG02890">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035605_0_1_2"/>
    <dbReference type="OrthoDB" id="254740at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06251">
        <property type="entry name" value="M14_ASTE_ASPA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055438">
        <property type="entry name" value="AstE_AspA_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043795">
        <property type="entry name" value="N-alpha-Ac-DABA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053138">
        <property type="entry name" value="N-alpha-Ac-DABA_deacetylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37326">
        <property type="entry name" value="BLL3975 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37326:SF1">
        <property type="entry name" value="BLL3975 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24827">
        <property type="entry name" value="AstE_AspA_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039012">
        <property type="entry name" value="ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Succinylglutamate desuccinylase/Aspartoacylase catalytic" evidence="5">
        <location>
            <begin position="45"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI50227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="336" mass="36525" checksum="94A672FAFB9A601C" modified="2017-05-10" version="1">MTETFTYDGGAVVPGETANIRYTVSETYLGDPARIPVTIINGERPGPTLFLSAAIHGDELNGIEIVREVAHEWNHDDLAGTLVCLPVLNVPGFIAQERYLPVYDRDLNRSFPGNDSGTSAKRIADRIFSNFIAPCDFGLDFHTSTRGRTNMLHVRADVDDADAARLAYAFGSNVVISSDGPSGTLRREATDAATPTVTVELGEAHRFQRKLIDAALEGVLSVMAELGMRDSAPVKWPGWRTVIDGSDEKTWLRADAGGLVEMYHDRGDIVEAGDRICTITTPFKTESTVVEAPFTGLLVGVLENPLVYPGNPLCHLVALDDPTTRAARASRSETRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYG7</accession>
    <name>A0A1U7EYG7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4400A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYG7"/>
    <dbReference type="EnsemblBacteria" id="CAI50291">
        <property type="protein sequence ID" value="CAI50291"/>
        <property type="gene ID" value="NP_4400A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_4400A"/>
    <dbReference type="HOGENOM" id="CLU_213589_1_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055934">
        <property type="entry name" value="DUF7512"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24352">
        <property type="entry name" value="DUF7512"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="50" mass="5186" checksum="809C4A3F3AFCC2B9" modified="2017-05-10" version="1">MLDTVLASPAVQAIAVLALVSVEAVALYLGYGVVEKRAAPALFERIEAEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYG9</accession>
    <name>A0A1U7EYG9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4340A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323877.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYG9"/>
    <dbReference type="STRING" id="348780.NP_4340A"/>
    <dbReference type="EnsemblBacteria" id="CAI50261">
        <property type="protein sequence ID" value="CAI50261"/>
        <property type="gene ID" value="NP_4340A"/>
    </dbReference>
    <dbReference type="GeneID" id="43500098"/>
    <dbReference type="KEGG" id="nph:NP_4340A"/>
    <dbReference type="HOGENOM" id="CLU_3075447_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="52" mass="5771" checksum="857A7F58376041B8" modified="2017-05-10" version="1">MLRTLLGLLVTLLKLPFKLLMLPFKIVSLLVSLLVYTVLLLAAAGILYVFVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYJ5</accession>
    <name>A0A1U7EYJ5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycine-rich protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4404A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323908.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYJ5"/>
    <dbReference type="STRING" id="348780.NP_4404A"/>
    <dbReference type="EnsemblBacteria" id="CAI50293">
        <property type="protein sequence ID" value="CAI50293"/>
        <property type="gene ID" value="NP_4404A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702051"/>
    <dbReference type="KEGG" id="nph:NP_4404A"/>
    <dbReference type="eggNOG" id="arCOG06238">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135433_0_0_2"/>
    <dbReference type="OrthoDB" id="343204at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="134" mass="13933" checksum="657DFE183D6C58A3" modified="2017-05-10" version="1">MTDEEEGESGEEKSFRERVEEIRQQRAEEGDGEGDGEGLSREEKMQEMMGGGGGPGGMGGGGGMGGSPFAQMMGGMMGGGGPGAGAGGPPGMGGSPEGEGSDNEELVREVRKVRDELHDIRREMERVADALEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYJ9</accession>
    <name>A0A1U7EYJ9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Sensor/bat box HTH-10 family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_4406A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323909.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYJ9"/>
    <dbReference type="STRING" id="348780.NP_4406A"/>
    <dbReference type="EnsemblBacteria" id="CAI50294">
        <property type="protein sequence ID" value="CAI50294"/>
        <property type="gene ID" value="NP_4406A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702052"/>
    <dbReference type="KEGG" id="nph:NP_4406A"/>
    <dbReference type="eggNOG" id="arCOG02278">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010057_3_0_2"/>
    <dbReference type="OrthoDB" id="106505at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031803">
        <property type="entry name" value="BAT_GAF/HTH-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007050">
        <property type="entry name" value="HTH_bacterioopsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013656">
        <property type="entry name" value="PAS_4"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236:SF1">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15915">
        <property type="entry name" value="BAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13185">
        <property type="entry name" value="GAF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04967">
        <property type="entry name" value="HTH_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08448">
        <property type="entry name" value="PAS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PAS" evidence="3">
        <location>
            <begin position="8"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="529" mass="58006" checksum="0A7BCFC62E754B1D" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYK9</accession>
    <name>A0A1U7EYK9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_4474A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323943.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYK9"/>
    <dbReference type="EnsemblBacteria" id="CAI50328">
        <property type="protein sequence ID" value="CAI50328"/>
        <property type="gene ID" value="NP_4474A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702073"/>
    <dbReference type="KEGG" id="nph:NP_4474A"/>
    <dbReference type="eggNOG" id="arCOG04649">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_154325_1_0_2"/>
    <dbReference type="OrthoDB" id="240603at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043816">
        <property type="entry name" value="DUF5798"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19111">
        <property type="entry name" value="DUF5798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="81"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="24"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="81"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="105" mass="11287" checksum="F1495F355536BCAC" modified="2017-05-10" version="1">MGLGGATKKLQKVADMGEELYSKMGELREQILEVRETVQETNQRVAALENRLDQQEAILEALAEAEGIDVDELLTEVAIEEAEPADGDSEAADDAAETIPSADDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYQ5</accession>
    <name>A0A1U7EYQ5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">Probable deoxyhypusine synthase</fullName>
            <shortName evidence="11">DHS</shortName>
            <ecNumber evidence="6 11">2.5.1.46</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">dhs</name>
        <name evidence="11" type="synonym">dys</name>
        <name evidence="13" type="ordered locus">NP_4578A</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 11">Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>[eIF5A protein]-L-lysine + spermidine = [eIF5A protein]-deoxyhypusine + propane-1,3-diamine</text>
            <dbReference type="Rhea" id="RHEA:33299"/>
            <dbReference type="Rhea" id="RHEA-COMP:10143"/>
            <dbReference type="Rhea" id="RHEA-COMP:10144"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57484"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:82657"/>
            <dbReference type="EC" id="2.5.1.46"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 11">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 11">Protein modification; eIF5A hypusination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 11">Belongs to the deoxyhypusine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.46" evidence="6 11"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323995.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYQ5"/>
    <dbReference type="STRING" id="348780.NP_4578A"/>
    <dbReference type="EnsemblBacteria" id="CAI50380">
        <property type="protein sequence ID" value="CAI50380"/>
        <property type="gene ID" value="NP_4578A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702665"/>
    <dbReference type="KEGG" id="nph:NP_4578A"/>
    <dbReference type="eggNOG" id="arCOG04142">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039781_1_0_2"/>
    <dbReference type="OrthoDB" id="17730at2157"/>
    <dbReference type="UniPathway" id="UPA00354"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034038">
        <property type="term" value="F:deoxyhypusine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.910.10:FF:000010">
        <property type="entry name" value="Deoxyhypusine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.910.10">
        <property type="entry name" value="Deoxyhypusine synthase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022899">
        <property type="entry name" value="Deoxyhypus_synthase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002773">
        <property type="entry name" value="Deoxyhypusine_synthase"/>
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        <property type="entry name" value="dhys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003052">
        <property type="entry name" value="PRK03971.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11703">
        <property type="entry name" value="DEOXYHYPUSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11703:SF0">
        <property type="entry name" value="DEOXYHYPUSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01916">
        <property type="entry name" value="DS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0386">Hypusine biosynthesis</keyword>
    <keyword evidence="8 11" id="KW-0520">NAD</keyword>
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    <keyword evidence="7 11 13" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAI50380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="326" mass="35843" checksum="D67D182CB7D41B18" modified="2017-05-10" version="1">MDADESRDHVVPGSDGELQTDDVRGYDFRGTFDLESLIDSYATTGFQATHLAEAVDIATGMQDDGATVFLTCTSNIVSSGLREVVAALIREGHVDVLITTSGSLTEDVIKTAKPFKMGEWDADEAKLREEGINRLGNIFVPSDRYVWLEEYLYEFFDDFFAEEKTRTPTAFARELGERLDDEDSVLKQAADNDVPVYCPALTDAEVGNFLYYYRQMYDESVGIEILDDYERLIEDGLDADTTGLIAVGDGVPKHHAIMTNLFRGGAEYAVYISTGMEGDGSLSGAPPTEAVSWGKIKSEETNYTQIEAEATLVFPLLVAAAFDMDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYT5</accession>
    <name>A0A1U7EYT5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4618A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A1U7EYT5"/>
    <dbReference type="STRING" id="348780.NP_4618A"/>
    <dbReference type="EnsemblBacteria" id="CAI50400">
        <property type="protein sequence ID" value="CAI50400"/>
        <property type="gene ID" value="NP_4618A"/>
    </dbReference>
    <dbReference type="GeneID" id="77383470"/>
    <dbReference type="KEGG" id="nph:NP_4618A"/>
    <dbReference type="HOGENOM" id="CLU_3227915_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="43" mass="4922" checksum="89FA595DA2CC4B32" modified="2017-05-10" version="1">MHALTTAWMCPRGRAIITSYDFAEQPENAPEPREDGTLVQRFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYV6</accession>
    <name>A0A1U7EYV6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TrmB family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_4688A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324049.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYV6"/>
    <dbReference type="EnsemblBacteria" id="CAI50435">
        <property type="protein sequence ID" value="CAI50435"/>
        <property type="gene ID" value="NP_4688A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702143"/>
    <dbReference type="KEGG" id="nph:NP_4688A"/>
    <dbReference type="eggNOG" id="arCOG04498">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1067961_0_0_2"/>
    <dbReference type="OrthoDB" id="350231at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055860">
        <property type="entry name" value="DUF7437"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24218">
        <property type="entry name" value="DUF7437"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator TrmB N-terminal" evidence="1">
        <location>
            <begin position="34"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7437" evidence="2">
        <location>
            <begin position="110"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="208" mass="22413" checksum="2864E0BD80933320" modified="2017-05-10" version="1">MPPRSVSKTPSQDLEAGINQLATVGRLLEHPALARVYVYACYYGPATRPQIKEALAIPKTTVYDHVETLEALGIVTLEGDRPVEVTAEPLQITTDGIVVTPTILHAVALQEVDEDVSYFVERYGVGKVAAAVRQAGLHYAGQITQRMVADPLDIPTGEAISIVLALRPALAVGQEYDPHFDVIFPDIAGDIEFETDLDVAAPLPNSES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYX5</accession>
    <name>A0A1U7EYX5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Probable NhaC-type sodium/proton antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">nhaC2</name>
        <name evidence="11" type="synonym">tp52</name>
        <name evidence="11" type="ordered locus">NP_4702A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the NhaC Na(+)/H(+) (TC 2.A.35) antiporter family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324056.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYX5"/>
    <dbReference type="STRING" id="348780.NP_4702A"/>
    <dbReference type="EnsemblBacteria" id="CAI50442">
        <property type="protein sequence ID" value="CAI50442"/>
        <property type="gene ID" value="NP_4702A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703338"/>
    <dbReference type="KEGG" id="nph:NP_4702A"/>
    <dbReference type="eggNOG" id="arCOG02010">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033405_1_0_2"/>
    <dbReference type="OrthoDB" id="213699at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004770">
        <property type="entry name" value="Na/H_antiport_NhaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018461">
        <property type="entry name" value="Na/H_Antiport_NhaC-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052180">
        <property type="entry name" value="NhaC_Na-H+_Antiporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00931">
        <property type="entry name" value="antiport_nhaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33451">
        <property type="entry name" value="MALATE-2H(+)/NA(+)-LACTATE ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33451:SF3">
        <property type="entry name" value="MALATE-2H(+)_NA(+)-LACTATE ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03553">
        <property type="entry name" value="Na_H_antiporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="50"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="120"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="245"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="270"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="315"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="361"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="442"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="Na+/H+ antiporter NhaC-like C-terminal" evidence="10">
        <location>
            <begin position="171"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI50442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="478" mass="49248" checksum="0F48E1783558BC7B" modified="2017-05-10" version="1">MALDFTPKTVDDIAPERRPSFRLALAPVLAVVLFLGVGSAVLGLDPHVPLLWSIVFTGAFGQYLGYSWPDLYEGISNGLLMGLQAILIIFTIYALIATWVDAGTIPAMMYFGLELLSPRVFLPVAAILAAVVAFAIGSSWTTVGTLGVAFVGIGTGLGVPGPMTVGAVISGAYAGDKQSPLSDTTNLAAGVTNTSLYDHIYRMRTGTAIAFGLAVLGFAALGLQAGGAVPADRIAEIQTAVDGTYNLSLFVFAPLAITFGLALRGYPALPVLVVGTFAGVCTAILVQGAAFVPAWEVFMSGTAPETGSELVNDLLATGGLTGSAWTITIVVAALVLGGLLEHTGVLAVLAHHLSQGVQSSGGLIAGTGVSAILINALTAQQYMSIVLPGMTLRNLYEEFGLDSDELSRAVEAAGTPTGALIPWHAGGVFMASATGVATLSYAPYYLFGFLSPLVLFAMALSGRGVPTTSRDEHPHRAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYX7</accession>
    <name>A0A1U7EYX7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4716A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYX7"/>
    <dbReference type="EnsemblBacteria" id="CAI50449">
        <property type="protein sequence ID" value="CAI50449"/>
        <property type="gene ID" value="NP_4716A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_4716A"/>
    <dbReference type="HOGENOM" id="CLU_2802380_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053463">
        <property type="entry name" value="Brz_Regulator"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23454">
        <property type="entry name" value="Zn_ribbon_Brz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="67" mass="7319" checksum="AEB98BB57FA5539E" modified="2017-05-10" version="1">MGDQVVACPICDTHINFSVVELTADKEIKFVKDTEIEPSDDYDVHEAQCPSGHSFYYAAGDVDPLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ18</accession>
    <name>A0A1U7EZ18_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Lrp/AsnC family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_4828A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324117.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ18"/>
    <dbReference type="STRING" id="348780.NP_4828A"/>
    <dbReference type="EnsemblBacteria" id="CAI50505">
        <property type="protein sequence ID" value="CAI50505"/>
        <property type="gene ID" value="NP_4828A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702189"/>
    <dbReference type="KEGG" id="nph:NP_4828A"/>
    <dbReference type="eggNOG" id="arCOG01580">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_5_4_2"/>
    <dbReference type="OrthoDB" id="14434at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050684">
        <property type="entry name" value="HTH-Siroheme_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019885">
        <property type="entry name" value="Tscrpt_reg_HTH_AsnC-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413:SF4">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ASNC FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13404">
        <property type="entry name" value="HTH_AsnC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00519">
        <property type="entry name" value="HTH_ASNC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI50505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="137" mass="15509" checksum="111966537C44ED04" modified="2017-05-10" version="1">MDDLDREILAILRRDSRTPYTEIAEQVGTSEGTVRNRVERLLEEGIIERFTIATRTGNVKAMIEISVAVDVDTGDIADRMTEWREVDFVWQVSGEEDVVVVVDTTDTGTLNDLITRARELDEVVSTKTRLILAERRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ28</accession>
    <name>A0A1U7EZ28_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein uS17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">rps17</name>
        <name evidence="7" type="ordered locus">NP_4872A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uS17 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ28"/>
    <dbReference type="STRING" id="348780.NP_4872A"/>
    <dbReference type="EnsemblBacteria" id="CAI50527">
        <property type="protein sequence ID" value="CAI50527"/>
        <property type="gene ID" value="NP_4872A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_4872A"/>
    <dbReference type="eggNOG" id="arCOG04096">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073626_0_3_2"/>
    <dbReference type="OrthoDB" id="10698at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00364">
        <property type="entry name" value="Ribosomal_uS17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01345_A">
        <property type="entry name" value="Ribosomal_uS17_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000266">
        <property type="entry name" value="Ribosomal_uS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028333">
        <property type="entry name" value="Ribosomal_uS17_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019978">
        <property type="entry name" value="Ribosomal_uS17_archaeal"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006345">
        <property type="entry name" value="PRK08572.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03630">
        <property type="entry name" value="uS17_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S11 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744:SF9">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00366">
        <property type="entry name" value="Ribosomal_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00973">
        <property type="entry name" value="RIBOSOMALS17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI50527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="114" mass="12926" checksum="45EA9442BBCDD62C" modified="2017-05-10" version="1">MAIGLDVTQPPEPENPEEYDYETCPFYGELPVRGQVIEGTVVSTDMERTVIVEREYDVNVPKYDRLMKRRSRIPAHVPEVLEPLSVDDEVKIAETRPLSKTKSHVVVEVTEGDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ90</accession>
    <name>A0A1U7EZ90_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5022A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ90"/>
    <dbReference type="STRING" id="348780.NP_5022A"/>
    <dbReference type="EnsemblBacteria" id="CAI50602">
        <property type="protein sequence ID" value="CAI50602"/>
        <property type="gene ID" value="NP_5022A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_5022A"/>
    <dbReference type="eggNOG" id="arCOG07770">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151708_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="139" mass="14898" checksum="62E812CE225387C5" modified="2017-05-10" version="1">MSGVRVCQHMSRTLFGMLGAVMALFPDAVLDAFEAIALESPGEYTPRPWLRSGVRAEGLVYAALAVVGGRAYTGLLYVVGAVGAVVVAAPRQYLAFGARLAYEDGDSVEWTDGFVRAARWLGVCCVLLAVRGVRTERED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZA1</accession>
    <name>A0A1U7EZA1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF5808 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_5046A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324224.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZA1"/>
    <dbReference type="STRING" id="348780.NP_5046A"/>
    <dbReference type="EnsemblBacteria" id="CAI50614">
        <property type="protein sequence ID" value="CAI50614"/>
        <property type="gene ID" value="NP_5046A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702237"/>
    <dbReference type="KEGG" id="nph:NP_5046A"/>
    <dbReference type="eggNOG" id="arCOG04718">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2340042_0_0_2"/>
    <dbReference type="OrthoDB" id="234861at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043831">
        <property type="entry name" value="DUF5808"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19124">
        <property type="entry name" value="DUF5808"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5808" evidence="2">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="91" mass="10256" checksum="5338EA10C0893CA9" modified="2017-05-10" version="1">MTDKPQSGELFGIPYNFERPSISRMLSAYWQPGEGMLVKKPFGIGYTLNLANWRSWLVLGVAAALFYQQQQSGGDEELDDDEEPVEVVVED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZA9</accession>
    <name>A0A1U7EZA9_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Branched-chain-amino-acid aminotransferase</fullName>
            <shortName evidence="15">BCAT</shortName>
            <ecNumber evidence="15">2.6.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ilvE1</name>
        <name evidence="15" type="synonym">ilvE</name>
        <name evidence="16" type="ordered locus">NP_5036A</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 15">Acts on leucine, isoleucine and valine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>L-isoleucine + 2-oxoglutarate = (S)-3-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:18321"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 15">
            <text>L-valine + 2-oxoglutarate = 3-methyl-2-oxobutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24813"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 15">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 4/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5 15">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 4/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 15">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 4/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 15">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.42" evidence="15"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324219.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZA9"/>
    <dbReference type="STRING" id="348780.NP_5036A"/>
    <dbReference type="EnsemblBacteria" id="CAI50609">
        <property type="protein sequence ID" value="CAI50609"/>
        <property type="gene ID" value="NP_5036A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702869"/>
    <dbReference type="KEGG" id="nph:NP_5036A"/>
    <dbReference type="eggNOG" id="arCOG02297">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020844_3_1_2"/>
    <dbReference type="OrthoDB" id="6469at2157"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00058"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00073"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00062"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052656">
        <property type="term" value="F:L-isoleucine-2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052654">
        <property type="term" value="F:L-leucine-2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052655">
        <property type="term" value="F:L-valine-2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01557">
        <property type="entry name" value="BCAT_beta_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.10.10:FF:000001">
        <property type="entry name" value="Branched-chain-amino-acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="entry name" value="D-amino Acid Aminotransferase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005785">
        <property type="entry name" value="B_amino_transI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033939">
        <property type="entry name" value="BCAT_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050571">
        <property type="entry name" value="Class-IV_PLP-Dep_Aminotrnsfr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01122">
        <property type="entry name" value="ilvE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005146">
        <property type="entry name" value="PRK06606.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42743">
        <property type="entry name" value="AMINO-ACID AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42743:SF11">
        <property type="entry name" value="AMINODEOXYCHORISMATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="D-aminoacid aminotransferase-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 15 16" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="11 15" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="10 15" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 15 16" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU364094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAI50609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="312" mass="34925" checksum="B4F696E0A32EF137" modified="2017-05-10" version="1">MAFEEMDVDTIWMDGEFVDWDEAQTHVLTHSLHYGTGVFEGARCYDTEDGPALFRWEEHLDRLFESAKVLDHDIDHTHEEITDATLRLIREQGLESCYIRPLVYYGYNSLGVSPEDCPTKTMVACWPWGAYLGEDAIENGVDVMVSTWRKHASSQIPTNVKATGPYINSMLAGEEARRNGYVEAIVLNKEGKVAEGPGENIFMVNDGEIYTTGLSESILDGITRDTVIELARDLGYTVHDEAAIGRGQLYTADELFFTGTAAEVTPIRSVDDTEIGNGGRGPVTEEIQQRFFDLVDGKLDGYDEWFLPVETE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZB0</accession>
    <name>A0A1U7EZB0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GNAT family acetyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5068A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324235.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZB0"/>
    <dbReference type="STRING" id="348780.NP_5068A"/>
    <dbReference type="EnsemblBacteria" id="CAI50625">
        <property type="protein sequence ID" value="CAI50625"/>
        <property type="gene ID" value="NP_5068A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702245"/>
    <dbReference type="KEGG" id="nph:NP_5068A"/>
    <dbReference type="eggNOG" id="arCOG00831">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_19_1_2"/>
    <dbReference type="OrthoDB" id="55684at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43072:SF52">
        <property type="entry name" value="GCN5-RELATED N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43072">
        <property type="entry name" value="N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="82"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="243" mass="27433" checksum="F385C3989ADA32ED" modified="2017-05-10" version="1">METSEQPTFETEAGEEIYRFVERHGTAARHRVREVTSLPAEEFQDALEQLQERGYLEDDGGTLQVALDVGSIETYSTDDIDYTVRPAQQTDFEGLIDTIRDVTAEDTYIVAESIAEQLLYEDAVNRHNTAESRVFFVATVDGDVVGWSHLDLPQLDKLQSTAQLTVGVRGEHQGHGIGTRLLERALDWAEANGYRKVYNSVPTTNDEAIAFLEERGWETEGVRKNHYTIGDEHVDEVMLAYTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZB9</accession>
    <name>A0A1U7EZB9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PQQ repeat protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5062A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324232.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZB9"/>
    <dbReference type="STRING" id="348780.NP_5062A"/>
    <dbReference type="EnsemblBacteria" id="CAI50622">
        <property type="protein sequence ID" value="CAI50622"/>
        <property type="gene ID" value="NP_5062A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702244"/>
    <dbReference type="KEGG" id="nph:NP_5062A"/>
    <dbReference type="eggNOG" id="arCOG02556">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027480_4_1_2"/>
    <dbReference type="OrthoDB" id="136681at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043041">
        <property type="term" value="P:amino acid activation for nonribosomal peptide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.630">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052091">
        <property type="entry name" value="Beta-ala_Activ/Resist"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002372">
        <property type="entry name" value="PQQ_rpt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44394">
        <property type="entry name" value="BETA-ALANINE-ACTIVATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44394:SF1">
        <property type="entry name" value="BETA-ALANINE-ACTIVATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13360">
        <property type="entry name" value="PQQ_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyrrolo-quinoline quinone repeat" evidence="1">
        <location>
            <begin position="36"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrrolo-quinoline quinone repeat" evidence="1">
        <location>
            <begin position="158"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrrolo-quinoline quinone repeat" evidence="1">
        <location>
            <begin position="268"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="377" mass="39388" checksum="166022D77AB41205" modified="2017-05-10" version="1">MDTPSRRSFLLAGGSFAIGVGAGLGIAHRDDVLPSRVVWAFETTGRIVSSPTVGDGTVYVGSDDSTVHSVSTSDGTRRTTARLHTKAGVFSSPALGADGGLYVGSTDTAIYGLFSSPGARDEWRFETTDWVTASPAVADGTVYVGSTDGTMYALNAETGAEQWRFETGDGIVSSPAVVDGTVYVGSTDGTVYALDADSGTDRWQFDTEGRVYSSPTVADGTVYVGSYDANIYAIDTTDGVEQWQFETGDSIWSSPTVLEETLWIGSKDAALYAVATEDGSEQWRFPTGGEVNSSPTVAGETVYVGSDDANLYAVAASDGSERWTFETGGAVQSSPTVADGTVYVGSGDSTLYALDADETGSSEGSRAELRTLGYFAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZF0</accession>
    <name>A0A1U7EZF0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF8119 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_5140A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324271.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZF0"/>
    <dbReference type="STRING" id="348780.NP_5140A"/>
    <dbReference type="EnsemblBacteria" id="CAI50661">
        <property type="protein sequence ID" value="CAI50661"/>
        <property type="gene ID" value="NP_5140A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702269"/>
    <dbReference type="KEGG" id="nph:NP_5140A"/>
    <dbReference type="eggNOG" id="arCOG06361">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_192661_0_0_2"/>
    <dbReference type="OrthoDB" id="198557at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058432">
        <property type="entry name" value="DUF8119"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26436">
        <property type="entry name" value="DUF8119"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="DUF8119" evidence="2">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="76" mass="8750" checksum="CE61C2D8A6252694" modified="2017-05-10" version="1">MGFTDSIREHVREHRTGMVHDLMFAVVWVAIVNFLFSAVFTDAPSWAFYMFMLAGIPAYFGFFFSLSMARAQQDSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZI1</accession>
    <name>A0A1U7EZI1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Probable glycosyltransferase, type 2</fullName>
            <ecNumber evidence="1">2.4.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gtl5</name>
        <name evidence="1" type="ordered locus">NP_5222A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.4.-.-" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324312.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZI1"/>
    <dbReference type="STRING" id="348780.NP_5222A"/>
    <dbReference type="EnsemblBacteria" id="CAI50702">
        <property type="protein sequence ID" value="CAI50702"/>
        <property type="gene ID" value="NP_5222A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702301"/>
    <dbReference type="KEGG" id="nph:NP_5222A"/>
    <dbReference type="eggNOG" id="arCOG04794">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056498_0_0_2"/>
    <dbReference type="OrthoDB" id="123709at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="373" mass="40529" checksum="03C612BDC55C0256" modified="2017-05-10" version="1">MEYVQERVTTLHDFDGATPEAPVDRTTVVVPMTEREHAGLAAERVLSALETVEPARVLVPLRAPADRLEAVVDWLDGFDVPLEVLWCDGPRLAGLLSDSGLDGQRGKGRDVWLALGVASESEYVVVHDADAKTYDAGHVPKLLFPLAEGKQFSKGYYARVENDRLYGRLFRLLYRPLVEALRRDDDAAVLAYLDSFRYALAGEFAMTGSLARQMRLQRGWGLEVGTLGEAFRLAGTEGSAQVDLGVHEHDHRSVSGPSGLSEMAEQVAGALLRAVEENGGDPEYGRLQESYRETAERLVDDYALDAAFNGLAYDRGGEREQVDAYAAAVARPDADTRLPAWTDAAVTPQEVSDAAAADLAATVSAAEPRSAQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZJ4</accession>
    <name>A0A1U7EZJ4_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">L-threonine-O-3-phosphate decarboxylase</fullName>
            <ecNumber evidence="6">2.6.1.9</ecNumber>
            <ecNumber evidence="5">4.1.1.81</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">cobD</name>
        <name evidence="18" type="ordered locus">NP_5302A</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Decarboxylates L-threonine-O-3-phosphate to yield (R)-1-amino-2-propanol O-2-phosphate, the precursor for the linkage between the nucleotide loop and the corrin ring in cobalamin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-histidinol phosphate + 2-oxoglutarate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23744"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:57980"/>
            <dbReference type="EC" id="2.6.1.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>O-phospho-L-threonine + H(+) = (R)-1-aminopropan-2-yl phosphate + CO2</text>
            <dbReference type="Rhea" id="RHEA:11492"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58563"/>
            <dbReference type="ChEBI" id="CHEBI:58675"/>
            <dbReference type="EC" id="4.1.1.81"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 7/9.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. Histidinol-phosphate aminotransferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.9" evidence="6"/>
    <dbReference type="EC" id="4.1.1.81" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324351.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZJ4"/>
    <dbReference type="STRING" id="348780.NP_5302A"/>
    <dbReference type="EnsemblBacteria" id="CAI50742">
        <property type="protein sequence ID" value="CAI50742"/>
        <property type="gene ID" value="NP_5302A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702618"/>
    <dbReference type="KEGG" id="nph:NP_5302A"/>
    <dbReference type="eggNOG" id="arCOG04273">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_3_2_2"/>
    <dbReference type="OrthoDB" id="39225at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004400">
        <property type="term" value="F:histidinol-phosphate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048472">
        <property type="term" value="F:threonine-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005860">
        <property type="entry name" value="CobD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050106">
        <property type="entry name" value="HistidinolP_aminotransfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01140">
        <property type="entry name" value="L_thr_O3P_dcar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43643:SF6">
        <property type="entry name" value="HISTIDINOL-PHOSPHATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43643">
        <property type="entry name" value="HISTIDINOL-PHOSPHATE AMINOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="12" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="18" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="17">
        <location>
            <begin position="21"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAI50742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="337" mass="35745" checksum="19AD58F93094ABE7" modified="2017-05-10" version="1">MDPDSIDAVDRVPHGSSDDPDVLDLSANINPEVPDGCRAVYREAFADARSYPNDSYPEYRAAAADYVGCAADDVVPTAGGLAAMRLAIGTVVGPGDRVLVPAPSFGEYAREVRLAGGEPDIVDYGTLLDRSPEEYAMAVVCTPNNPTGDCYDADTLRAFADRCAEVGTVLLADEAFLGFTDRASLAGREGVVVARSLTKLFGLPGLRAGFAVATGELGDRLQTARPAWSLGGPAAAVGAHAMADTAFVSDTRRRIDRERAHLRDRLAAHGFEVFPSEAPFLLCDVGGPPDGLLAACREHGVVLRDATTFRGLDRHVRIAVRTREATETLCETLDVVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZM1</accession>
    <name>A0A1U7EZM1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7692 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5336A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324368.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZM1"/>
    <dbReference type="STRING" id="348780.NP_5336A"/>
    <dbReference type="EnsemblBacteria" id="CAI50759">
        <property type="protein sequence ID" value="CAI50759"/>
        <property type="gene ID" value="NP_5336A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702338"/>
    <dbReference type="KEGG" id="nph:NP_5336A"/>
    <dbReference type="eggNOG" id="arCOG08180">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_192653_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056109">
        <property type="entry name" value="DUF7692"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24743">
        <property type="entry name" value="DUF7692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7692" evidence="1">
        <location>
            <begin position="10"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="93" mass="10442" checksum="9DA55CECA003B200" modified="2017-05-10" version="1">MADQEVPGSVRIRTGDGNEWRFDAMEKAAEFYDCNRSNAVAFACDDVAQLVEAAHEVLARDDLTEQQRREMAETLSTRTTSFEVSVRVDANVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILT3</accession>
    <name>Q3ILT3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3318A</name>
        <name evidence="3" type="ordered locus">NP_7042A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>PL23</name>
    </geneLocation>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
            <strain evidence="3">Gabara</strain>
            <plasmid evidence="3">PL23</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49750.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CR936259">
        <property type="protein sequence ID" value="CAI50937.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILT3"/>
    <dbReference type="STRING" id="348780.NP_3318A"/>
    <dbReference type="EnsemblBacteria" id="CAI49750">
        <property type="protein sequence ID" value="CAI49750"/>
        <property type="gene ID" value="NP_3318A"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI50937">
        <property type="protein sequence ID" value="CAI50937"/>
        <property type="gene ID" value="NP_7042A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3318A"/>
    <dbReference type="KEGG" id="nph:NP_7042A"/>
    <dbReference type="eggNOG" id="arCOG06171">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2177949_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49750.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50937.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="109" mass="11791" checksum="6C32C845A6EB2E62" modified="2005-11-08" version="1">MALLSFPPVLLHELTHYAVARLRTDDAAFEAEVLGSEARAVWRPLDSPLWRFLAFLAPTLFGALLAGLWLASGTSFEGWRAVAGVGLALYTLPSVADIRGALGRQQAQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILU3</accession>
    <name>Q3ILU3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3298A</name>
        <name evidence="3" type="ordered locus">NP_7022A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>PL23</name>
    </geneLocation>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
            <strain evidence="3">Gabara</strain>
            <plasmid evidence="3">PL23</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CR936259">
        <property type="protein sequence ID" value="CAI50927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323361.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILU3"/>
    <dbReference type="STRING" id="348780.NP_3298A"/>
    <dbReference type="EnsemblBacteria" id="CAI49740">
        <property type="protein sequence ID" value="CAI49740"/>
        <property type="gene ID" value="NP_3298A"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI50927">
        <property type="protein sequence ID" value="CAI50927"/>
        <property type="gene ID" value="NP_7022A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703471"/>
    <dbReference type="KEGG" id="nph:NP_3298A"/>
    <dbReference type="KEGG" id="nph:NP_7022A"/>
    <dbReference type="HOGENOM" id="CLU_1302659_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="211" mass="23818" checksum="289CFA227D2D5F11" modified="2005-11-08" version="1">MDSETQEHMDAQEDRQLTASSQQAVMSAAAEKQHRKLENSHFLNELRDSDLGDSELFEWLEDEHPTWFSGAHAVSNRGDTWDDEADLLMFNKRERALAENRPGRLLRGRPFLLATAQGAETPQLDAYDQPGIPGDRQYWKRKTADKESARRPMSSEEQSRLYGAAEVAADLMALSKNAAGLDATTTATTETRVRREEQEEGAASRLGRFYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM06</accession>
    <name>Q3IM06_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_6164A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM06"/>
    <dbReference type="EnsemblBacteria" id="CAI50862">
        <property type="protein sequence ID" value="CAI50862"/>
        <property type="gene ID" value="NP_6164A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_6164A"/>
    <dbReference type="HOGENOM" id="CLU_3338563_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="37" mass="4219" checksum="2F8419D9293F15E1" modified="2005-11-08" version="1">MIRTGADQTDARSILEEYGPRTDIQEATRMHSNALNR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-09-05" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM07</accession>
    <name>NRDR_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcriptional repressor NrdR</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nrdR</name>
        <name type="ordered locus">NP_6162A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>PL131</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Negatively regulates transcription of bacterial ribonucleotide reductase nrd genes and operons by binding to NrdR-boxes.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NrdR family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324460.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM07"/>
    <dbReference type="SMR" id="Q3IM07"/>
    <dbReference type="EnsemblBacteria" id="CAI50861">
        <property type="protein sequence ID" value="CAI50861"/>
        <property type="gene ID" value="NP_6162A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694633"/>
    <dbReference type="KEGG" id="nph:NP_6162A"/>
    <dbReference type="HOGENOM" id="CLU_108412_0_0_2"/>
    <dbReference type="OrthoDB" id="212442at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00440">
        <property type="entry name" value="NrdR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055173">
        <property type="entry name" value="NrdR-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003796">
        <property type="entry name" value="RNR_NrdR-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00244">
        <property type="entry name" value="transcriptional regulator NrdR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30455">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR NRDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30455:SF2">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR NRDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03477">
        <property type="entry name" value="ATP-cone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22811">
        <property type="entry name" value="Zn_ribbon_NrdR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0678">Repressor</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000249028" description="Transcriptional repressor NrdR">
        <location>
            <begin position="1"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-cone" evidence="1">
        <location>
            <begin position="49"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="zinc finger region" evidence="1">
        <location>
            <begin position="3"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="162" mass="18307" checksum="5345B72357E31486" modified="2005-11-08" version="1">MNCPDCGNGRTRVIDTGASSDGASVRRRRECQRCSFRFTTYERPEWKSLQVKKRDGTIESFDQQKLRTGIERAVEKRGVAETTVTALVDDIESELQDREARIVSSSLIGELVSENLRTLDKVAYIRFVSVYKAFSEPQEFLKELDAVLGAELDDFEASNSSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM44</accession>
    <name>Q3IM44_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_6078A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324423.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM44"/>
    <dbReference type="EnsemblBacteria" id="CAI50819">
        <property type="protein sequence ID" value="CAI50819"/>
        <property type="gene ID" value="NP_6078A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694704"/>
    <dbReference type="KEGG" id="nph:NP_6078A"/>
    <dbReference type="HOGENOM" id="CLU_184175_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="95" mass="9781" checksum="6D4DD0A1FA3FEA75" modified="2005-11-08" version="1">MTNNTLTTADRIAMLGGGSLILCGIAVMGLVNELAGAPHIPVEEEGVIVATPVVAPELRAYVVGFGLVVWLFYGLYKLAAAPGGDGERERASPTA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IPL7</accession>
    <name>RL21_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein eL21</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">50S ribosomal protein L21e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl21e</name>
        <name type="ordered locus">NP_3684A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL21 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323551.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IPL7"/>
    <dbReference type="SMR" id="Q3IPL7"/>
    <dbReference type="STRING" id="348780.NP_3684A"/>
    <dbReference type="EnsemblBacteria" id="CAI49933">
        <property type="protein sequence ID" value="CAI49933"/>
        <property type="gene ID" value="NP_3684A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703131"/>
    <dbReference type="KEGG" id="nph:NP_3684A"/>
    <dbReference type="eggNOG" id="arCOG04129">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103610_1_1_2"/>
    <dbReference type="OrthoDB" id="6295at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.70">
        <property type="entry name" value="Ribosomal protein L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00369">
        <property type="entry name" value="Ribosomal_eL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001147">
        <property type="entry name" value="Ribosomal_eL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022856">
        <property type="entry name" value="Ribosomal_eL21_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018259">
        <property type="entry name" value="Ribosomal_eL21_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036948">
        <property type="entry name" value="Ribosomal_eL21_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003303">
        <property type="entry name" value="PRK04306.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01157">
        <property type="entry name" value="Ribosomal_L21e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01171">
        <property type="entry name" value="RIBOSOMAL_L21E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000243373" description="Large ribosomal subunit protein eL21">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="97" mass="10704" checksum="0AEEB0488F9E7378" modified="2005-11-08" version="1">MPSSNGPRQATRNKLKNDARERGTSPPQRSIEEYDDGEKVHLKLDPSVPNGQFHPRFNGRTGTVVGEQGDAFKVEIEDGNVTKTVIAAPAHLRRQQA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IPW7</accession>
    <name>TOP6A_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Type 2 DNA topoisomerase 6 subunit A</fullName>
            <ecNumber evidence="1">5.6.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Type II DNA topoisomerase VI subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">top6A</name>
        <name type="ordered locus">NP_3480A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Relaxes both positive and negative superturns and exhibits a strong decatenase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Heterotetramer of two Top6A and two Top6B chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TOP6A family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323451.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IPW7"/>
    <dbReference type="SMR" id="Q3IPW7"/>
    <dbReference type="STRING" id="348780.NP_3480A"/>
    <dbReference type="EnsemblBacteria" id="CAI49831">
        <property type="protein sequence ID" value="CAI49831"/>
        <property type="gene ID" value="NP_3480A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703275"/>
    <dbReference type="KEGG" id="nph:NP_3480A"/>
    <dbReference type="eggNOG" id="arCOG04143">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037229_1_0_2"/>
    <dbReference type="OrthoDB" id="5866at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00223">
        <property type="entry name" value="TOPRIM_TopoIIB_SPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1360.10:FF:000011">
        <property type="entry name" value="Type 2 DNA topoisomerase 6 subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00132">
        <property type="entry name" value="Top6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002815">
        <property type="entry name" value="Spo11/TopoVI_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013049">
        <property type="entry name" value="Spo11/TopoVI_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036078">
        <property type="entry name" value="Spo11/TopoVI_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049333">
        <property type="entry name" value="Topo_VI_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004085">
        <property type="entry name" value="TopoVI_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034136">
        <property type="entry name" value="TOPRIM_Topo6A/Spo11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003332">
        <property type="entry name" value="PRK04342.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10848">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN SPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10848:SF0">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN SPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21180">
        <property type="entry name" value="TOP6A-Spo11_Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20768">
        <property type="entry name" value="Topo_VI_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04406">
        <property type="entry name" value="TP6A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01550">
        <property type="entry name" value="TOP6AFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01552">
        <property type="entry name" value="TPISMRASE6A"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56726">
        <property type="entry name" value="DNA topoisomerase IV, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52041">
        <property type="entry name" value="TOPO_IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0799">Topoisomerase</keyword>
    <feature type="chain" id="PRO_1000018316" description="Type 2 DNA topoisomerase 6 subunit A">
        <location>
            <begin position="1"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="Topo IIA-type catalytic" evidence="2">
        <location>
            <begin position="9"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="2">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01385"/>
        </source>
    </evidence>
    <sequence length="368" mass="42118" checksum="3E7A61D8D7A000EE" modified="2005-11-08" version="1">MSSESDTDTEDEIARERLIELASDFYDQFAEGEVPTMEIPTRTKSNIVFDEDKDVWVYGDRTSTRSANSVRGAQKLLKAIYTIEFLADQLEQGRSSTLRELYYLSESWDEERAQFNDQDESNQLVEDLEIVSKVTREDFHMRPEESGATIMGPLYLREQTRRGEREIHCQKDVGEGGYQIPNNPDTIEFLDNDADFVLCVETGGMRDRLVENGFDEEHNVIIVHLKGQPARATRRITKRLHDDLDLPVVVFCDGDPWSYRIYASVAYGSIKSAHLSEYLATPEAEYIGIQPADIVEYDLPTDPLSDSDINALESELDDPRFQDDYWREQIELQLDIGKKAEQQALASRGLDFVTETYLPERLGEMGVL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IQA5</accession>
    <name>DEOC_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Deoxyribose-phosphate aldolase</fullName>
            <shortName evidence="1">DERA</shortName>
            <ecNumber evidence="1">4.1.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">2-deoxy-D-ribose 5-phosphate aldolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphodeoxyriboaldolase</fullName>
            <shortName evidence="1">Deoxyriboaldolase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">deoC</name>
        <name type="ordered locus">NP_3200A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes a reversible aldol reaction between acetaldehyde and D-glyceraldehyde 3-phosphate to generate 2-deoxy-D-ribose 5-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-deoxy-D-ribose 5-phosphate = D-glyceraldehyde 3-phosphate + acetaldehyde</text>
            <dbReference type="Rhea" id="RHEA:12821"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:62877"/>
            <dbReference type="EC" id="4.1.2.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; 2-deoxy-D-ribose 1-phosphate degradation; D-glyceraldehyde 3-phosphate and acetaldehyde from 2-deoxy-alpha-D-ribose 1-phosphate: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DeoC/FbaB aldolase family. DeoC type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.4" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323313.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IQA5"/>
    <dbReference type="SMR" id="Q3IQA5"/>
    <dbReference type="STRING" id="348780.NP_3200A"/>
    <dbReference type="EnsemblBacteria" id="CAI49691">
        <property type="protein sequence ID" value="CAI49691"/>
        <property type="gene ID" value="NP_3200A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702663"/>
    <dbReference type="KEGG" id="nph:NP_3200A"/>
    <dbReference type="eggNOG" id="arCOG04320">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053595_0_0_2"/>
    <dbReference type="OrthoDB" id="31145at2157"/>
    <dbReference type="UniPathway" id="UPA00002">
        <property type="reaction ID" value="UER00468"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004139">
        <property type="term" value="F:deoxyribose-phosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006018">
        <property type="term" value="P:2-deoxyribose 1-phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009264">
        <property type="term" value="P:deoxyribonucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00114">
        <property type="entry name" value="DeoC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011343">
        <property type="entry name" value="DeoC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002915">
        <property type="entry name" value="DeoC/FbaB/LacD_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028581">
        <property type="entry name" value="DeoC_typeI"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00126">
        <property type="entry name" value="deoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10889">
        <property type="entry name" value="DEOXYRIBOSE-PHOSPHATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10889:SF1">
        <property type="entry name" value="DEOXYRIBOSE-PHOSPHATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01791">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001357">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01133">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0704">Schiff base</keyword>
    <feature type="chain" id="PRO_0000231575" description="Deoxyribose-phosphate aldolase">
        <location>
            <begin position="1"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with acetaldehyde" evidence="1">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00114"/>
        </source>
    </evidence>
    <sequence length="215" mass="21991" checksum="814069B0B93EFC70" modified="2005-11-08" version="1">MDRDTFAARIDHTVLGPETAPGDVEAVLDAAAEYGMNACVPPCYVEAASDYAPDVTLVSVCGFPHGQHATETKATEAETVWQAGADEIDVVINVGRLRGGDTDAVRAEIEQVVAAVPVPVKVIVEAPLLSASELRTACELAADADAAYLKTATGFNGDATVAAVETMAEYLPVKASGGIGSFEAAVEMFDAGADRIGASAGAAIVDGFDPAAFET</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IRP7</accession>
    <name>THIC_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphomethylpyrimidine synthase</fullName>
            <ecNumber evidence="1">4.1.99.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Hydroxymethylpyrimidine phosphate synthase</fullName>
            <shortName evidence="1">HMP-P synthase</shortName>
            <shortName evidence="1">HMP-phosphate synthase</shortName>
            <shortName evidence="1">HMPP synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Thiamine biosynthesis protein ThiC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thiC</name>
        <name type="ordered locus">NP_2210A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + S-adenosyl-L-methionine = 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + CO + 5'-deoxyadenosine + formate + L-methionine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:24840"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:17245"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58354"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="EC" id="4.1.99.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ThiC family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.17" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322823.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IRP7"/>
    <dbReference type="SMR" id="Q3IRP7"/>
    <dbReference type="STRING" id="348780.NP_2210A"/>
    <dbReference type="EnsemblBacteria" id="CAI49196">
        <property type="protein sequence ID" value="CAI49196"/>
        <property type="gene ID" value="NP_2210A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703244"/>
    <dbReference type="KEGG" id="nph:NP_2210A"/>
    <dbReference type="eggNOG" id="arCOG02741">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013181_2_1_2"/>
    <dbReference type="OrthoDB" id="335406at2157"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070284">
        <property type="term" value="F:phosphomethylpyrimidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.540:FF:000001">
        <property type="entry name" value="Phosphomethylpyrimidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.540">
        <property type="entry name" value="Radical SAM ThiC family, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00089">
        <property type="entry name" value="ThiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037509">
        <property type="entry name" value="ThiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038521">
        <property type="entry name" value="ThiC/Bza_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002817">
        <property type="entry name" value="ThiC/BzaA/B"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006763">
        <property type="entry name" value="PRK09284.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009895">
        <property type="entry name" value="PRK13352.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00190">
        <property type="entry name" value="thiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30557:SF1">
        <property type="entry name" value="PHOSPHOMETHYLPYRIMIDINE SYNTHASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30557">
        <property type="entry name" value="THIAMINE BIOSYNTHESIS PROTEIN THIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01964">
        <property type="entry name" value="ThiC_Rad_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00407">
        <property type="entry name" value="phosphomethylpyrimidine_syntha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01114">
        <property type="entry name" value="phosphomethylpyrimidine_syntha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00113">
        <property type="entry name" value="Radical_SAM_Phosphomethylpyrim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000242329" description="Phosphomethylpyrimidine synthase">
        <location>
            <begin position="1"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="427"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="427"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="180"/>
            <end position="182"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="221"/>
            <end position="224"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="408"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="411"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="416"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="477" mass="51518" checksum="DF463569F6AD6505" modified="2005-11-08" version="1">MTTQLQHARGGTVTDAMERVAERENRDAEFVRQQVADGQAVIPANHGHDALDPMIIGREFATKVNANIGNSEPASGPEAELQKLHTAVHYGADTVMDLSTGGDLDTIRTENIAYSPVPVGTVPIYEAVTRVDDASDLTPELLLDVVEKQAEQGVDYMTLHAGVLAEHLPLTDGRTTGIVSRGGSILARWMEEHGEQNPLYERFDDLCEILAAYDVTVSLGDGLRPGSLADASDAAQFAELDTLGELTRRAWDHGVQVMVEGPGHVPMDEIADNVERQQDVCDGAPFYVLGPLVTDVAPGYDHITSAIGATEAARAGAAMLCYVTPKEHLGLPDAEDVRDGLAAYRIAAHSADVANGRPGARDWDDAVSEARYAFDWRRQFELALDPGRARNYHDQTLPEDNYKEARFCSMCGVEFCSMRIDQDAREAGDGMDGLESRTDLDSSAAAAVNRPPTGVHRAEKLDDIPCPVAEDDVAADD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-01-10" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IRT1</accession>
    <name>HIS8_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Histidinol-phosphate aminotransferase</fullName>
            <ecNumber evidence="1">2.6.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Imidazole acetol-phosphate transaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisC</name>
        <name type="ordered locus">NP_2140A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-histidinol phosphate + 2-oxoglutarate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23744"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:57980"/>
            <dbReference type="EC" id="2.6.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 7/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. Histidinol-phosphate aminotransferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.9" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322789.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IRT1"/>
    <dbReference type="SMR" id="Q3IRT1"/>
    <dbReference type="STRING" id="348780.NP_2140A"/>
    <dbReference type="EnsemblBacteria" id="CAI49161">
        <property type="protein sequence ID" value="CAI49161"/>
        <property type="gene ID" value="NP_2140A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702816"/>
    <dbReference type="KEGG" id="nph:NP_2140A"/>
    <dbReference type="eggNOG" id="arCOG04273">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_3_3_2"/>
    <dbReference type="OrthoDB" id="9929at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00012"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004400">
        <property type="term" value="F:histidinol-phosphate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01023">
        <property type="entry name" value="HisC_aminotrans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005861">
        <property type="entry name" value="HisP_aminotrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050106">
        <property type="entry name" value="HistidinolP_aminotransfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01141">
        <property type="entry name" value="hisC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43643:SF6">
        <property type="entry name" value="HISTIDINOL-PHOSPHATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43643">
        <property type="entry name" value="HISTIDINOL-PHOSPHATE AMINOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0032">Aminotransferase</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000153502" description="Histidinol-phosphate aminotransferase">
        <location>
            <begin position="1"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01023"/>
        </source>
    </evidence>
    <sequence length="355" mass="38236" checksum="CEF24EF51C66F398" modified="2005-11-08" version="1">MQPRDLSHHVAYEAGRGIEEVARELGLDPDDLVKLSSNENPFGPSPKAAEAIREHADRVNSYPKAAATDLRGEIGDLLSVDAAQVWLGNGGDGALDYLARAMLDPDDGVLVPDPGFAYYGMSARYHHGQVNSYPIEKDDDFEQSPDAILESYDGERIVYLTSPHNPTGSEVSLSDVEAVAEGTDEETLVVVDEAYGEFADSPSAVALVEERDDVAVLRTFSKAYGLAGVRLGYAVVPGEWADAYARVNTPFAASEIACRAGLAALEDDAHVEKTVETARWAREYIRSTLECRTYESAGNFVLCAVGDGGEVAEAAQKEGVIVRDTTSFGLPSCIRITCGTREETKRAVEVVNDLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-03-24" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IRT5</accession>
    <name>Y2132_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0173 metal-dependent hydrolase NP_2132A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">NP_2132A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0173 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322785.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IRT5"/>
    <dbReference type="SMR" id="Q3IRT5"/>
    <dbReference type="STRING" id="348780.NP_2132A"/>
    <dbReference type="EnsemblBacteria" id="CAI49157">
        <property type="protein sequence ID" value="CAI49157"/>
        <property type="gene ID" value="NP_2132A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701360"/>
    <dbReference type="KEGG" id="nph:NP_2132A"/>
    <dbReference type="eggNOG" id="arCOG00497">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070010_4_0_2"/>
    <dbReference type="OrthoDB" id="28313at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00457">
        <property type="entry name" value="UPF0173"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022877">
        <property type="entry name" value="UPF0173"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050114">
        <property type="entry name" value="UPF0173_UPF0282_UlaG_hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001911">
        <property type="entry name" value="PRK00685.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43546:SF3">
        <property type="entry name" value="UPF0173 METAL-DEPENDENT HYDROLASE MJ1163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43546">
        <property type="entry name" value="UPF0173 METAL-DEPENDENT HYDROLASE MJ1163-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000367237" description="UPF0173 metal-dependent hydrolase NP_2132A">
        <location>
            <begin position="1"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00457"/>
        </source>
    </evidence>
    <sequence length="241" mass="25575" checksum="03541ABDE0C5CC21" modified="2005-11-08" version="1">MELTWYGHSTWHVSVGSTDLLIDPFFGNPKTDLEPSDLDSPDYVLLTHGHEDHVAHAGAFSDATLVATPELVGYAEDNFGFEDAVGGMGMNLGGSVECGDAWVTMTRADHSNGIETGYETSAGMPAGFVVSESEPQRASDAANTAFYHAGDTSLMVEMREVIGPYLEPDFAALPAGDHFTMGPEQAAIAADWLGADYAAPMHYDTFPPIEIDTDEFVDALNATGADAEAVVLDGDESFTLG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-09-02" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IRZ3</accession>
    <name>UREG_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Urease accessory protein UreG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ureG</name>
        <name type="ordered locus">NP_2014A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Facilitates the functional incorporation of the urease nickel metallocenter. This process requires GTP hydrolysis, probably effectuated by UreG.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. UreD, UreF and UreG form a complex that acts as a GTP-hydrolysis-dependent molecular chaperone, activating the urease apoprotein by helping to assemble the nickel containing metallocenter of UreC. The UreE protein probably delivers the nickel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIMIBI class G3E GTPase family. UreG subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322727.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IRZ3"/>
    <dbReference type="SMR" id="Q3IRZ3"/>
    <dbReference type="STRING" id="348780.NP_2014A"/>
    <dbReference type="EnsemblBacteria" id="CAI49098">
        <property type="protein sequence ID" value="CAI49098"/>
        <property type="gene ID" value="NP_2014A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703454"/>
    <dbReference type="KEGG" id="nph:NP_2014A"/>
    <dbReference type="eggNOG" id="arCOG01231">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072144_1_0_2"/>
    <dbReference type="OrthoDB" id="812at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043419">
        <property type="term" value="P:urea catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01389">
        <property type="entry name" value="UreG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004400">
        <property type="entry name" value="UreG"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00101">
        <property type="entry name" value="ureG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31715">
        <property type="entry name" value="UREASE ACCESSORY PROTEIN G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31715:SF0">
        <property type="entry name" value="UREASE ACCESSORY PROTEIN G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005624">
        <property type="entry name" value="Ni-bind_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0996">Nickel insertion</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000347462" description="Urease accessory protein UreG">
        <location>
            <begin position="1"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="13"/>
            <end position="20"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01389"/>
        </source>
    </evidence>
    <sequence length="206" mass="21951" checksum="CB1492D5AAEA1CA9" modified="2005-11-08" version="1">MGHRDVATVGLGGPVGSGKTAMVKRLVPRLDAAGYNVGVIANDIMTQEDADRLRESFEGILPPDLVEGVETGACPHTGIREDPSMNIAAIDEFTESYPDLDVVLLESGGDNLAATFNPELADYFIFVISVAEGDDIPRKRGPGVTQADLLVVNKTDLAPHVDADLEVMRRDAETVRGDAPTCFTDCKAEEGIDGVVDHIEEGVLFA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-03-03" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ISJ9</accession>
    <name>F16PA_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fructose-1,6-bisphosphatase class 1</fullName>
            <shortName evidence="1">FBPase class 1</shortName>
            <ecNumber evidence="1">3.1.3.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">D-fructose-1,6-bisphosphate 1-phosphohydrolase class 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fbp</name>
        <name type="ordered locus">NP_1592A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="EC" id="3.1.3.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FBPase class 1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.11" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322521.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ISJ9"/>
    <dbReference type="SMR" id="Q3ISJ9"/>
    <dbReference type="STRING" id="348780.NP_1592A"/>
    <dbReference type="EnsemblBacteria" id="CAI48887">
        <property type="protein sequence ID" value="CAI48887"/>
        <property type="gene ID" value="NP_1592A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702693"/>
    <dbReference type="KEGG" id="nph:NP_1592A"/>
    <dbReference type="eggNOG" id="arCOG04603">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039977_3_0_2"/>
    <dbReference type="OrthoDB" id="146513at2157"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042132">
        <property type="term" value="F:fructose 1,6-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030388">
        <property type="term" value="P:fructose 1,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005986">
        <property type="term" value="P:sucrose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01855">
        <property type="entry name" value="FBPase_class1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044015">
        <property type="entry name" value="FBPase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000146">
        <property type="entry name" value="FBPase_class-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033391">
        <property type="entry name" value="FBPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028343">
        <property type="entry name" value="FBPtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020548">
        <property type="entry name" value="Fructose_bisphosphatase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006785">
        <property type="entry name" value="PRK09293.3-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006786">
        <property type="entry name" value="PRK09293.3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11556">
        <property type="entry name" value="FRUCTOSE-1,6-BISPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11556:SF35">
        <property type="entry name" value="SEDOHEPTULOSE-1,7-BISPHOSPHATASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00316">
        <property type="entry name" value="FBPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18913">
        <property type="entry name" value="FBPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500210">
        <property type="entry name" value="FBPtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000904">
        <property type="entry name" value="FBPtase_SBPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00115">
        <property type="entry name" value="F16BPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00124">
        <property type="entry name" value="FBPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000364776" description="Fructose-1,6-bisphosphatase class 1">
        <location>
            <begin position="1"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="89"/>
            <end position="92"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01855"/>
        </source>
    </evidence>
    <sequence length="283" mass="30157" checksum="D5797BC01B0F4AB7" modified="2005-11-08" version="1">MADAIDDIVDVVVSTAPDVRDGLTGRRVYEADENPSGERQLEADVYADELLEARLLDVDAVGSYASEERESVIEADEGGRYHVAADPLDGSSNLKSNNTMGTLFGVYEEPLPAAGDALVAAGYVLYGPITTLITARDGTVTESVIDEDGTRTVVTDDLTLPSDPVVYGFGGRVPNWTPAFADYVESVASELKLRYGGAMIGDVNQVLTYGGVFGYPGLEDRPEGKLRLLFEGHPIAAIVEAAGGASSDGDGSLLKKEPEQLHERTPLFVGNDEYIERLEAALP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IT99</accession>
    <name>Q3IT99_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cobalt-precorrin-7 C5-methyltransferase</fullName>
            <ecNumber evidence="8">2.1.1.289</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cbiE</name>
        <name evidence="8" type="synonym">cobL1</name>
        <name evidence="8" type="ordered locus">NP_1088A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.289" evidence="8"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322271.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IT99"/>
    <dbReference type="STRING" id="348780.NP_1088A"/>
    <dbReference type="EnsemblBacteria" id="CAI48635">
        <property type="protein sequence ID" value="CAI48635"/>
        <property type="gene ID" value="NP_1088A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702569"/>
    <dbReference type="KEGG" id="nph:NP_1088A"/>
    <dbReference type="eggNOG" id="arCOG00650">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089162_1_0_2"/>
    <dbReference type="OrthoDB" id="42238at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11644">
        <property type="entry name" value="Precorrin-6Y-MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012818">
        <property type="entry name" value="CbiE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050714">
        <property type="entry name" value="Cobalamin_biosynth_MTase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02467">
        <property type="entry name" value="CbiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004457">
        <property type="entry name" value="PRK05787.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43182">
        <property type="entry name" value="COBALT-PRECORRIN-6B C(15)-METHYLTRANSFERASE (DECARBOXYLATING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43182:SF1">
        <property type="entry name" value="COBALT-PRECORRIN-7 C(5)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="3 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="7">
        <location>
            <begin position="27"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="10"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI48635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="245" mass="26355" checksum="358D1481C609B0D4" modified="2005-11-08" version="1">MTDPVEFPDEPAAAAASTPESEAGEPVYAVGIGPGTTEYLTRRGARAIREADVVVGFETVVDFVSEELTGDALTCGYDDEPAVLAQFSERIADGDRGTAVLMGDPNHSGYQFVGKVEAAVDSPVEVIPGISSLQVAASRARTPMEETTFVTLHKSGPIEADLRRLERDVGDRNLLVLPRPFDWMPERIAHRLRAAGAPDCEALVFERLTHEDEAVTKTSLSSLAADIETRAETAFSDLSVLAVRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-18" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ITY6</accession>
    <name>PFDB_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Prefoldin subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">GimC subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pfdB</name>
        <name type="ordered locus">NP_0612A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Molecular chaperone capable of stabilizing a range of proteins. Seems to fulfill an ATP-independent, HSP70-like function in archaeal de novo protein folding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterohexamer of two alpha and four beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322033.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ITY6"/>
    <dbReference type="SMR" id="Q3ITY6"/>
    <dbReference type="STRING" id="348780.NP_0612A"/>
    <dbReference type="EnsemblBacteria" id="CAI48397">
        <property type="protein sequence ID" value="CAI48397"/>
        <property type="gene ID" value="NP_0612A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703018"/>
    <dbReference type="KEGG" id="nph:NP_0612A"/>
    <dbReference type="eggNOG" id="arCOG01342">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131909_0_1_2"/>
    <dbReference type="OrthoDB" id="204796at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00307">
        <property type="entry name" value="PfdB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012713">
        <property type="entry name" value="PfdB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02338">
        <property type="entry name" value="gimC_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20903:SF0">
        <property type="entry name" value="PREFOLDIN SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20903">
        <property type="entry name" value="PREFOLDIN SUBUNIT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000232444" description="Prefoldin subunit beta">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="19"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="20"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="114"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="131" mass="14455" checksum="58C11D09402CD7A6" modified="2005-11-08" version="1">MQGNLPPEAQEKIEELQDLQETAQQVAQQKQQAETQLRESETALETLDDIEGDTQMYQEVGELLIETDYDEAYENLEEKVDSLEVRVETLTKQEERVQDQFEGLQEELQEMLQGGAGGGPMGPGGPGAGGA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-03-18" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IU11</accession>
    <name>LIPA_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lipoyl synthase</fullName>
            <ecNumber evidence="1">2.8.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Lip-syn</fullName>
            <shortName evidence="1">LS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Lipoate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Lipoic acid synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Sulfur insertion protein LipA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lipA</name>
        <name type="ordered locus">NP_0562A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[[Fe-S] cluster scaffold protein carrying a second [4Fe-4S](2+) cluster] + N(6)-octanoyl-L-lysyl-[protein] + 2 oxidized [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine + 4 H(+) = [[Fe-S] cluster scaffold protein] + N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + 4 Fe(3+) + 2 hydrogen sulfide + 2 5'-deoxyadenosine + 2 L-methionine + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:16585"/>
            <dbReference type="Rhea" id="RHEA-COMP:9928"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:14568"/>
            <dbReference type="Rhea" id="RHEA-COMP:14569"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:33722"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:78809"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="EC" id="2.8.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the radical SAM superfamily. Lipoyl synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.8" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IU11"/>
    <dbReference type="SMR" id="Q3IU11"/>
    <dbReference type="STRING" id="348780.NP_0562A"/>
    <dbReference type="EnsemblBacteria" id="CAI48372">
        <property type="protein sequence ID" value="CAI48372"/>
        <property type="gene ID" value="NP_0562A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_0562A"/>
    <dbReference type="eggNOG" id="arCOG00660">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033144_2_0_2"/>
    <dbReference type="OrthoDB" id="145957at2157"/>
    <dbReference type="UniPathway" id="UPA00538">
        <property type="reaction ID" value="UER00593"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016992">
        <property type="term" value="F:lipoate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00206">
        <property type="entry name" value="Lipoyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031691">
        <property type="entry name" value="LIAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003698">
        <property type="entry name" value="Lipoyl_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00510">
        <property type="entry name" value="lipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004019">
        <property type="entry name" value="PRK05481.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009544">
        <property type="entry name" value="PRK12928.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10949">
        <property type="entry name" value="LIPOYL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10949:SF0">
        <property type="entry name" value="LIPOYL SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16881">
        <property type="entry name" value="LIAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005963">
        <property type="entry name" value="Lipoyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00271">
        <property type="entry name" value="lipoyl_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01058">
        <property type="entry name" value="lipoyl_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000325322" description="Lipoyl synthase">
        <location>
            <begin position="1"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="53"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <sequence length="309" mass="34323" checksum="1B7FC3BE5C9574A6" modified="2005-11-08" version="1">MSSRRRKPEWLRTRPPSGRRFTEIKETLRDRDLNTVCEEANCPNLGDCWSGRDGPGTATFMLLGDRCSRGCNFCDVATGGMDPLDPDEPANVAEAVAEIGLDYVVLTSVDRDDLDDGGAGHFAETIREIKRRDTSVLVEALIPDFQGDTDAVDRIIDADPDVVAHNVETVERLQWPVRDRRAGYEQSLDVLRQIAAADGCYAKTSLMLGVGEYAHEVYRTLGDLSEVGVDIVTFGQYLQPSRSHLEVFEYVTPDTFDVWKRVAETEFGFLYCASGPMVRSSFKAGELFVEALERDGLDIEAARAAAERQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IU96</accession>
    <name>SUI1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein translation factor SUI1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">NP_0388A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SUI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321923.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IU96"/>
    <dbReference type="SMR" id="Q3IU96"/>
    <dbReference type="STRING" id="348780.NP_0388A"/>
    <dbReference type="EnsemblBacteria" id="CAI48285">
        <property type="protein sequence ID" value="CAI48285"/>
        <property type="gene ID" value="NP_0388A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703263"/>
    <dbReference type="KEGG" id="nph:NP_0388A"/>
    <dbReference type="eggNOG" id="arCOG04223">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082805_6_1_2"/>
    <dbReference type="OrthoDB" id="11182at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002188">
        <property type="term" value="P:translation reinitiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11567">
        <property type="entry name" value="YciH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.780.10:FF:000006">
        <property type="entry name" value="Protein translation factor SUI1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00604">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050318">
        <property type="entry name" value="DENR/SUI1_TIF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022851">
        <property type="entry name" value="SUI1_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005872">
        <property type="entry name" value="SUI1_arc_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002096">
        <property type="entry name" value="PRK00939.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01158">
        <property type="entry name" value="SUI1_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12789:SF0">
        <property type="entry name" value="DENSITY-REGULATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12789">
        <property type="entry name" value="DENSITY-REGULATED PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037511">
        <property type="entry name" value="Transl_init_SUI1_pro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="eIF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0810">Translation regulation</keyword>
    <feature type="chain" id="PRO_1000006440" description="Protein translation factor SUI1 homolog">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00604"/>
        </source>
    </evidence>
    <sequence length="97" mass="10701" checksum="A0F622D9E3A0A82C" modified="2005-11-08" version="1">MSEVCSTCGLPEELCVCEDVAKESQEINIRIDERRYGKEVTVIEGFDPQDVDLDSLSSDLKSKFACGGTVEDGSIELQGNHLGRVEDFLREKGFNVA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IUE5</accession>
    <name>SYV_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Valine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Valyl-tRNA synthetase</fullName>
            <shortName evidence="1">ValRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">valS</name>
        <name type="ordered locus">NP_0288A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Val) + L-valine + ATP = L-valyl-tRNA(Val) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:10704"/>
            <dbReference type="Rhea" id="RHEA-COMP:9672"/>
            <dbReference type="Rhea" id="RHEA-COMP:9708"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78537"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.9" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321874.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IUE5"/>
    <dbReference type="SMR" id="Q3IUE5"/>
    <dbReference type="STRING" id="348780.NP_0288A"/>
    <dbReference type="EnsemblBacteria" id="CAI48235">
        <property type="protein sequence ID" value="CAI48235"/>
        <property type="gene ID" value="NP_0288A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703460"/>
    <dbReference type="KEGG" id="nph:NP_0288A"/>
    <dbReference type="eggNOG" id="arCOG00808">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001493_0_2_2"/>
    <dbReference type="OrthoDB" id="23906at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004832">
        <property type="term" value="F:valine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006438">
        <property type="term" value="P:valyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07962">
        <property type="entry name" value="Anticodon_Ia_Val"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00817">
        <property type="entry name" value="ValRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000192">
        <property type="entry name" value="Valine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="entry name" value="Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02005">
        <property type="entry name" value="Val_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033705">
        <property type="entry name" value="Anticodon_Ia_Val"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022874">
        <property type="entry name" value="Valine-tRNA_ligase_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002303">
        <property type="entry name" value="Valyl-tRNA_ligase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009687">
        <property type="entry name" value="PRK13208.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00422">
        <property type="entry name" value="valS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946:SF93">
        <property type="entry name" value="VALINE--TRNA LIGASE, CHLOROPLASTIC_MITOCHONDRIAL 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946">
        <property type="entry name" value="VALYL-TRNA SYNTHETASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00986">
        <property type="entry name" value="TRNASYNTHVAL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000224629" description="Valine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="524"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="527"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02005"/>
        </source>
    </evidence>
    <sequence length="872" mass="99041" checksum="450FD04235C3DED2" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IUF7</accession>
    <name>AATD_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">A-type ATP synthase subunit D</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpD</name>
        <name type="ordered locus">NP_0264A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Has multiple subunits with at least A(3), B(3), C, D, E, F, H, I and proteolipid K(x).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase D subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321862.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IUF7"/>
    <dbReference type="SMR" id="Q3IUF7"/>
    <dbReference type="STRING" id="348780.NP_0264A"/>
    <dbReference type="EnsemblBacteria" id="CAI48223">
        <property type="protein sequence ID" value="CAI48223"/>
        <property type="gene ID" value="NP_0264A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702541"/>
    <dbReference type="KEGG" id="nph:NP_0264A"/>
    <dbReference type="eggNOG" id="arCOG04101">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069688_2_1_2"/>
    <dbReference type="OrthoDB" id="117390at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.3240:FF:000007">
        <property type="entry name" value="V-type ATP synthase subunit D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00271">
        <property type="entry name" value="ATP_synth_D_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002699">
        <property type="entry name" value="V_ATPase_D"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001542">
        <property type="entry name" value="PRK00373.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001545">
        <property type="entry name" value="PRK00373.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00309">
        <property type="entry name" value="V_ATPase_subD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11671">
        <property type="entry name" value="V-TYPE ATP SYNTHASE SUBUNIT D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01813">
        <property type="entry name" value="ATP-synt_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_1000059170" description="A-type ATP synthase subunit D">
        <location>
            <begin position="1"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="204"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="215"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="231" mass="26262" checksum="6B76365EBBFB792D" modified="2005-11-08" version="1">MSKDVKPTRKNLMAIEDRIELSERGHDTLEKKRDGLIMEFMDILDQAQDVRSDLDENYERAQRNINMARAMEGDVAVRGAAAALKEHPEITTQSKNIMGVVVPQIESTKVKKSLDQRGYGLVGTSARIDEAADAYEELLETIILAAEVETAMKKMLDEIEKTKRRVNALEFKLLPELHEAQEYIEQKLEEQEREEIFRMKKIKAKKEEEEEENPVADTESAAEAAVTEAGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ET99</accession>
    <name>A0A1U7ET99_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0046A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ET99"/>
    <dbReference type="STRING" id="348780.NP_0046A"/>
    <dbReference type="EnsemblBacteria" id="CAI48114">
        <property type="protein sequence ID" value="CAI48114"/>
        <property type="gene ID" value="NP_0046A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_0046A"/>
    <dbReference type="eggNOG" id="arCOG06286">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2949404_0_0_2"/>
    <dbReference type="OrthoDB" id="227858at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="59" mass="6894" checksum="5B170F869739A81C" modified="2017-05-10" version="1">MRTASEIQERIEKLESEYDDYDPPSSEYEDTAEVAVLRAIEELEWVLEEYDEDAGFTTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETE6</accession>
    <name>A0A1U7ETE6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">HTH-10 family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_0162A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321811.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETE6"/>
    <dbReference type="STRING" id="348780.NP_0162A"/>
    <dbReference type="EnsemblBacteria" id="CAI48172">
        <property type="protein sequence ID" value="CAI48172"/>
        <property type="gene ID" value="NP_0162A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700779"/>
    <dbReference type="KEGG" id="nph:NP_0162A"/>
    <dbReference type="eggNOG" id="arCOG02272">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099316_0_0_2"/>
    <dbReference type="OrthoDB" id="194721at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007050">
        <property type="entry name" value="HTH_bacterioopsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056486">
        <property type="entry name" value="HVO_2525_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236:SF1">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04967">
        <property type="entry name" value="HTH_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24279">
        <property type="entry name" value="HVO_2525_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HVO-2525 N-terminal" evidence="4">
        <location>
            <begin position="3"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="HTH bat-type" evidence="3">
        <location>
            <begin position="171"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI48172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="236" mass="26198" checksum="A9C637B99C4677CE" modified="2017-05-10" version="1">MIDLDIDMRQYDCPFIDTTDEVPVSFSAMQWHLDTSYGQLETRMIVKGESRETLTRGLQTLRDHGNITSCVSLKKKGDTATIRTVIEETDAMSVVQNHDGYITGPFNIEDGTEIWHVGFDDEATADRALSDLDDENDVAVESRTELTVAELFDVIENANAAHSFLDSCRALTDTERETLVAAAEAGYFETPRGASLDDLAEEFDVSKTAASMNLRRAERKLVEATIESMSSLRGVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETI7</accession>
    <name>A0A1U7ETI7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 9">Peptide chain release factor subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Translation termination factor aRF1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">arf1</name>
        <name evidence="9 11" type="synonym">prf1</name>
        <name evidence="11" type="ordered locus">NP_0270A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 9">Heterodimer of two subunits, one of which binds GTP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the eukaryotic release factor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321865.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETI7"/>
    <dbReference type="STRING" id="348780.NP_0270A"/>
    <dbReference type="EnsemblBacteria" id="CAI48226">
        <property type="protein sequence ID" value="CAI48226"/>
        <property type="gene ID" value="NP_0270A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703049"/>
    <dbReference type="KEGG" id="nph:NP_0270A"/>
    <dbReference type="eggNOG" id="arCOG01742">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035759_3_0_2"/>
    <dbReference type="OrthoDB" id="1011at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016149">
        <property type="term" value="F:translation release factor activity, codon specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.60:FF:000003">
        <property type="entry name" value="Peptide chain release factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.960.10:FF:000003">
        <property type="entry name" value="Peptide chain release factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.960.10">
        <property type="entry name" value="eRF1 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="entry name" value="eRF1 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00424">
        <property type="entry name" value="Rel_fact_arch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024049">
        <property type="entry name" value="eRF1_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_Pelota-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020918">
        <property type="entry name" value="Peptide_chain-rel_aRF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004403">
        <property type="entry name" value="Peptide_chain-rel_eRF1/aRF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03676">
        <property type="entry name" value="aRF1_eRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10113">
        <property type="entry name" value="PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03463">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55481">
        <property type="entry name" value="N-terminal domain of eukaryotic peptide chain release factor subunit 1, ERF1"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eRF1/Pelota-like N-terminal" evidence="10">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI48226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="413" mass="46357" checksum="226B70BC8CF7AF4B" modified="2017-05-10" version="1">MSEDDGEPSDKQKYEFQKVIEELDEYSGSGTQLVSIYVPEDRQISDVVQHVTQEHSEAANIKSKQTRTAVQDALKSLKDRLKYYDTFPPDRGMVLFSGAVDAGGGQTDMVTEVLESPPDPIQSFRYHCDSHFLTEPLQEMLGDKGLYGLVVLDRREANVGWLKGKRVEPVKSASSLVPGKQRKGGQSAQRFARLRLEAIDNFYQEVAEMADDLFVPKRHEMDGILVGGPSPTKDEFLDGDYLHHELQDMVLGKFDISYTDESGLYDLVDAAEDVLAETEVMKDKAEMEEFFEQLHDGQKATYGFDATRQNLMMGSVDRLLISEDLRKDVVTFDCGSREEYELIDRRADTPDHTCEDGSEGEAVEREDAIDHLMELAEQRGSDVKFISTDFEKGEQLLTAFGGIAGILRYSTGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETL9</accession>
    <name>A0A1U7ETL9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ATP-dependent RNA helicase/nuclease Hef</fullName>
            <ecNumber evidence="9">3.6.4.13</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">hef</name>
        <name evidence="9" type="ordered locus">NP_0336A</name>
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    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.4.13" evidence="9"/>
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        <property type="protein sequence ID" value="CAI48259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321897.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETL9"/>
    <dbReference type="STRING" id="348780.NP_0336A"/>
    <dbReference type="EnsemblBacteria" id="CAI48259">
        <property type="protein sequence ID" value="CAI48259"/>
        <property type="gene ID" value="NP_0336A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700821"/>
    <dbReference type="KEGG" id="nph:NP_0336A"/>
    <dbReference type="eggNOG" id="arCOG00872">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002513_3_1_2"/>
    <dbReference type="OrthoDB" id="9764at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:RNA helicase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20075">
        <property type="entry name" value="XPF_nuclease_XPF_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1320.20">
        <property type="entry name" value="hef helicase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006166">
        <property type="entry name" value="ERCC4_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041755">
        <property type="entry name" value="Hef_ID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010337">
        <property type="entry name" value="PRK13766.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14025">
        <property type="entry name" value="FANCONI ANEMIA GROUP M FANCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14025:SF20">
        <property type="entry name" value="FANCONI ANEMIA GROUP M PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02732">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14520">
        <property type="entry name" value="HHH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21210">
        <property type="entry name" value="RNA_helicase_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00891">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 9" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="30"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="362"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="529"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="495"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="545"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="563"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI48259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="845" mass="91853" checksum="747106AD71976494" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETP2</accession>
    <name>A0A1U7ETP2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0398A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321928.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETP2"/>
    <dbReference type="STRING" id="348780.NP_0398A"/>
    <dbReference type="EnsemblBacteria" id="CAI48290">
        <property type="protein sequence ID" value="CAI48290"/>
        <property type="gene ID" value="NP_0398A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700842"/>
    <dbReference type="KEGG" id="nph:NP_0398A"/>
    <dbReference type="eggNOG" id="arCOG04773">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1329428_0_0_2"/>
    <dbReference type="OrthoDB" id="304501at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="205" mass="23769" checksum="4AF2737889220568" modified="2017-05-10" version="1">MAWINPQDSKRSAWAGKSDAGSDADPATRSVYQRAVEANTPYRRRERVRLSGESVAEQYRSREYTDAAISYDTVVSAAGERRVVARGHLWGGDDDDQRYRIQYRRKGPPTETVPFDEYEVWVRYQFGTVTHIQKGEPVFKPSANPEEEKRTLAWDKLYASDRFRLIEAELVGNPELARYRLKEQEVWEAIRDVFRYDPKAFGVTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETV4</accession>
    <name>A0A1U7ETV4_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF5658 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0524A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETV4"/>
    <dbReference type="STRING" id="348780.NP_0524A"/>
    <dbReference type="EnsemblBacteria" id="CAI48353">
        <property type="protein sequence ID" value="CAI48353"/>
        <property type="gene ID" value="NP_0524A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_0524A"/>
    <dbReference type="eggNOG" id="arCOG04975">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_166555_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043717">
        <property type="entry name" value="DUF5658"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18902">
        <property type="entry name" value="DUF5658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="DUF5658" evidence="2">
        <location>
            <begin position="18"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="110" mass="11913" checksum="87F0BF9129F3DA08" modified="2017-05-10" version="1">MRASVRFFPVYSALLWALAVLLYGVGDLVTTVAGTRHEHVREAAPLTRRLFGPAPSAWRFGLFKLGLLGAFYAVTRTVVPPPYQPAVPAAIAVVGLVAVGNNLRVLWRVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETW5</accession>
    <name>A0A1U7ETW5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-binding protein MutS2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">mutS5a</name>
        <name evidence="4" type="synonym">mutS2</name>
        <name evidence="7" type="ordered locus">NP_0586A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Has ATPase and non-specific DNA-binding activities.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DNA mismatch repair MutS family. Archaeal Muts2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322020.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
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    <dbReference type="STRING" id="348780.NP_0586A"/>
    <dbReference type="EnsemblBacteria" id="CAI48384">
        <property type="protein sequence ID" value="CAI48384"/>
        <property type="gene ID" value="NP_0586A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702953"/>
    <dbReference type="KEGG" id="nph:NP_0586A"/>
    <dbReference type="eggNOG" id="arCOG02895">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026764_0_0_2"/>
    <dbReference type="OrthoDB" id="15514at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00971">
        <property type="entry name" value="MutS2_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012401">
        <property type="entry name" value="DNA-bd_MutS2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF125">
        <property type="entry name" value="DNA-BINDING PROTEIN MUTS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14520">
        <property type="entry name" value="HHH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029254">
        <property type="entry name" value="MutS_C_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helix-hairpin-helix DNA-binding motif class 1" evidence="5">
        <location>
            <begin position="4"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="domain" description="Helix-hairpin-helix DNA-binding motif class 1" evidence="5">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="6">
        <location>
            <begin position="474"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="481"/>
            <end position="488"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="667" mass="71915" checksum="BC63A57A1D058D57" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETW8</accession>
    <name>A0A1U7ETW8_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ORC1-type DNA replication protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">orc2</name>
        <name evidence="7" type="synonym">cdc6B</name>
        <name evidence="7" type="ordered locus">NP_0588A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in regulation of DNA replication.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the CDC6/cdc18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322021.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETW8"/>
    <dbReference type="STRING" id="348780.NP_0588A"/>
    <dbReference type="EnsemblBacteria" id="CAI48385">
        <property type="protein sequence ID" value="CAI48385"/>
        <property type="gene ID" value="NP_0588A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702591"/>
    <dbReference type="KEGG" id="nph:NP_0588A"/>
    <dbReference type="eggNOG" id="arCOG00467">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025112_3_0_2"/>
    <dbReference type="OrthoDB" id="53276at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01407">
        <property type="entry name" value="ORC1_type_DNA_replic_protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015163">
        <property type="entry name" value="Cdc6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055237">
        <property type="entry name" value="Cdc6_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050311">
        <property type="entry name" value="ORC1/CDC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014277">
        <property type="entry name" value="Orc1/Cdc6_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02928">
        <property type="entry name" value="orc1/cdc6 family replication initiation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001624">
        <property type="entry name" value="PRK00411.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001626">
        <property type="entry name" value="PRK00411.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763:SF26">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22703">
        <property type="entry name" value="Cdc6_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01074">
        <property type="entry name" value="Cdc6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cdc6 C-terminal" evidence="6">
        <location>
            <begin position="297"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="63"/>
            <end position="67"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="374" mass="41547" checksum="75256FAF1525F113" modified="2017-05-10" version="1">MADDDMLSWDESVFRDEGVLELDYLPEAFLHRDEQMETLKYALRPAVRGSRPLNVMARGPPGTGKTTAVQKLFDELAGVGGVETVRVNCQVDSTRYAVFSRLFESLFEYEPPASGVSFNKLFGQITNRLVEDDDVLVVALDDVNYLFYESEASDTLYSLLRAHESHSGAKIGVIVVSSDLDLNVIEELDGRVQSVFRPEDVYFSAYGERAIADILSERVERGFREGAVSARVLDRVAEHTADSGGDLRVGIDLLRRAGLNAEFRGSTEVAVADVDTAYEKAKYIHLSRRLQGLSESEAALVRVLTDHDGERAGAVYEAFSEETELGYTRYSEIVNKLEQVGVVDAEYTNVDGRGRSRELSLAYDADAVLERLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETX4</accession>
    <name>A0A1U7ETX4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Thermosome subunit 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ths2</name>
        <name evidence="7" type="ordered locus">NP_0570A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322012.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETX4"/>
    <dbReference type="STRING" id="348780.NP_0570A"/>
    <dbReference type="EnsemblBacteria" id="CAI48376">
        <property type="protein sequence ID" value="CAI48376"/>
        <property type="gene ID" value="NP_0570A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703258"/>
    <dbReference type="KEGG" id="nph:NP_0570A"/>
    <dbReference type="eggNOG" id="arCOG01258">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008891_7_3_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03343">
        <property type="entry name" value="cpn60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000017">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012714">
        <property type="entry name" value="Thermosome_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02339">
        <property type="entry name" value="thermosome_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="529"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="538"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="561" mass="58945" checksum="2FF01B00F099EF64" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU42</accession>
    <name>A0A1U7EU42_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC-type transport system ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">abc27a</name>
        <name evidence="5" type="ordered locus">NP_0748A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322101.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU42"/>
    <dbReference type="STRING" id="348780.NP_0748A"/>
    <dbReference type="EnsemblBacteria" id="CAI48465">
        <property type="protein sequence ID" value="CAI48465"/>
        <property type="gene ID" value="NP_0748A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702447"/>
    <dbReference type="KEGG" id="nph:NP_0748A"/>
    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OrthoDB" id="87732at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050763">
        <property type="entry name" value="ABC_transporter_ATP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025302">
        <property type="entry name" value="DrrA1/2-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711:SF15">
        <property type="entry name" value="ABC-TYPE MULTIDRUG TRANSPORT SYSTEM, ATPASE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13732">
        <property type="entry name" value="DrrA1-3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="5"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI48465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="315" mass="34896" checksum="A077A222BA4E0DEA" modified="2017-05-10" version="1">MEPAIRADELRKSYGDVDALDGLSFEVDRGEFFGLLGPNGAGKTTFINILVGLVRRDGGTAEVFGFDVEDDYQEARDRIGLAPQEFNVDRFFPIHEVLEHKAGYHGVPRGEAEERAAEALQTVGIWDKRETRFDWLSGGMKRRFLLARALVTDPDLLILDEPTAGVDVQLRRELWRVIEQLNAGGTTILLTTHYIEEAERLCDRVAIVDSGSIVEVATPDDLRDRGTDQLYIRLADTPTTVPEIDIDGLVEARLDGDTLVVTAQGGSSAAPALLRTLERQGHEVLDLDIRRASLEEVFVDMTRPGEEAEELGVVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU78</accession>
    <name>A0A1U7EU78_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Aminodeoxychorismate lyase</fullName>
            <ecNumber evidence="5">4.1.3.38</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">pabC</name>
        <name evidence="5" type="synonym">ilvE2</name>
        <name evidence="5" type="ordered locus">NP_0798A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.38" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322126.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU78"/>
    <dbReference type="STRING" id="348780.NP_0798A"/>
    <dbReference type="EnsemblBacteria" id="CAI48490">
        <property type="protein sequence ID" value="CAI48490"/>
        <property type="gene ID" value="NP_0798A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702870"/>
    <dbReference type="KEGG" id="nph:NP_0798A"/>
    <dbReference type="eggNOG" id="arCOG02297">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020844_3_0_2"/>
    <dbReference type="OrthoDB" id="196861at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008696">
        <property type="term" value="F:4-amino-4-deoxychorismate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046394">
        <property type="term" value="P:carboxylic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00449">
        <property type="entry name" value="PLPDE_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.10.10:FF:000002">
        <property type="entry name" value="D-alanine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="entry name" value="D-amino Acid Aminotransferase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018300">
        <property type="entry name" value="Aminotrans_IV_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050571">
        <property type="entry name" value="Class-IV_PLP-Dep_Aminotrnsfr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42743">
        <property type="entry name" value="AMINO-ACID AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42743:SF11">
        <property type="entry name" value="AMINODEOXYCHORISMATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="D-aminoacid aminotransferase-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00770">
        <property type="entry name" value="AA_TRANSFER_CLASS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI48490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="284" mass="30845" checksum="17B9F29E5887A930" modified="2017-05-10" version="1">MYYHVDGDLVDREDATVHVDDRGFRYGDAAFETCRAYGGEVFLWDRHRERLAETCRLLGMAEAVPEDLQGRIDETLQANGYADAYIRVSVSRGVQSGKLTPGPATDPTVVVYAKPLPRGGGDGENCWETDATVHTVETRRPPESVLPSAAKTHNYLNGILARIELRGTAADEALMCDTEGAVAEGATSNVFFVEEGTLKTPAKGALLPGITRATVLSLAESEGIPVEEGRYRPADIEAADEAFLTNTTWEVRPIATVDGTTVGGGETTRRLSRAYDELVETRCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU86</accession>
    <name>A0A1U7EU86_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">tRNA (Guanine(6)-N(2))-dimethyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.256</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">trm14</name>
        <name evidence="5" type="ordered locus">NP_0864A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.256" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322159.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU86"/>
    <dbReference type="STRING" id="348780.NP_0864A"/>
    <dbReference type="EnsemblBacteria" id="CAI48523">
        <property type="protein sequence ID" value="CAI48523"/>
        <property type="gene ID" value="NP_0864A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700982"/>
    <dbReference type="KEGG" id="nph:NP_0864A"/>
    <dbReference type="eggNOG" id="arCOG00048">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032119_3_0_2"/>
    <dbReference type="OrthoDB" id="7080at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008990">
        <property type="term" value="F:rRNA (guanine-N2-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070043">
        <property type="term" value="F:rRNA (guanine-N7-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160117">
        <property type="term" value="F:tRNA (guanine(6)-N2)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11715">
        <property type="entry name" value="THUMP_AdoMetMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2130.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000241">
        <property type="entry name" value="RlmKL-like_Mtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054170">
        <property type="entry name" value="RlmL_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47313">
        <property type="entry name" value="RIBOSOMAL RNA LARGE SUBUNIT METHYLTRANSFERASE K/L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47313:SF1">
        <property type="entry name" value="RIBOSOMAL RNA LARGE SUBUNIT METHYLTRANSFERASE K_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22020">
        <property type="entry name" value="RlmL_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01170">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143437">
        <property type="entry name" value="THUMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="THUMP" evidence="4">
        <location>
            <begin position="42"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI48523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="360" mass="39907" checksum="E870FBFB09515EBD" modified="2017-05-10" version="1">MQLLATTNGGLESVAAAELRELTGAEATRHHRGVVAFEGTVEDVYELHYRARSLHRIMAVLVDTPVAGLDDIYATTADMPVADRLPEATFGVVGTRHGTHEFTSMDVADRVGQAVIDAYREATGTRLPVDLDDPTVRLEAYLYDERFTLAVDLTGESLHRRPYRVCEHDAPLRGTLAYQLLRLADYSADDRLVDPMAGSATIPIEAALDATDAVPRPDLDPAFTVLPRFDGDRFRRRRAAHTRREAAVDITARDNQTQWRECARENREAAGFEDEVDIVAADAREASLDADCVVTNLPFGIRVTGDLRRLYSEFAERVTDGSVDRLVALTTKPGLLPLSPDEQHDIPYGRIDASVIVWRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU90</accession>
    <name>A0A1U7EU90_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0201 protein NP_0868A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0868A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0201 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322161.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU90"/>
    <dbReference type="STRING" id="348780.NP_0868A"/>
    <dbReference type="EnsemblBacteria" id="CAI48525">
        <property type="protein sequence ID" value="CAI48525"/>
        <property type="gene ID" value="NP_0868A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700984"/>
    <dbReference type="KEGG" id="nph:NP_0868A"/>
    <dbReference type="eggNOG" id="arCOG01043">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134829_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01112">
        <property type="entry name" value="UPF0201"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002739">
        <property type="entry name" value="PAB1135-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_uL5_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39652">
        <property type="entry name" value="UPF0201 PROTEIN TK1335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39652:SF1">
        <property type="entry name" value="UPF0201 PROTEIN TK1335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01877">
        <property type="entry name" value="RNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="RL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="134" mass="14628" checksum="1999CBE25563E788" modified="2017-05-10" version="1">MIYSVDVQITAPVNETEVTDRVADAIENLFPEAEIESHPGELMGTGHSMETFSERLHEQAILDSARGAFFSDLEGDTFSFRLKKQAAFQGVINFAVGSESELGDIHVRVKVNEPDAETYIDSVAPPTEDGAPVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUA3</accession>
    <name>A0A1U7EUA3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0890A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322172.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUA3"/>
    <dbReference type="STRING" id="348780.NP_0890A"/>
    <dbReference type="EnsemblBacteria" id="CAI48536">
        <property type="protein sequence ID" value="CAI48536"/>
        <property type="gene ID" value="NP_0890A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700989"/>
    <dbReference type="KEGG" id="nph:NP_0890A"/>
    <dbReference type="eggNOG" id="arCOG00499">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085057_0_0_2"/>
    <dbReference type="OrthoDB" id="53037at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42663:SF6">
        <property type="entry name" value="HYDROLASE C777.06C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42663">
        <property type="entry name" value="HYDROLASE C777.06C-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="63"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="294" mass="32397" checksum="D1422C0E032E169A" modified="2017-05-10" version="1">MVTVTVLGSGGNFPLPTPTCECRLCVEARSEGIPYARHGNNLYIEDLSAVVDAPEFVFENLNRAGVDEFSYLFLTHWHPDHTAGLRVVQSRPDASLFDDSDRGIIDTARSDVPTLVTTERVFERTCELYDGLRHFSEMGYVETHFLDDDPLVVDGIRVEAIPYSLSGNSDMDATAFVLFDGDTTVVLAPDDARYLDEAALPDDIDLAVFECGYFEQTPDGTPILADADAAVFDDELTHGEVLARIDRLAPERTLLTEIEHIYARSHDQLQALANGYDGIEFAYDGATVDASDGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUA5</accession>
    <name>A0A1U7EUA5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0880A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322167.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUA5"/>
    <dbReference type="STRING" id="348780.NP_0880A"/>
    <dbReference type="EnsemblBacteria" id="CAI48531">
        <property type="protein sequence ID" value="CAI48531"/>
        <property type="gene ID" value="NP_0880A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700987"/>
    <dbReference type="KEGG" id="nph:NP_0880A"/>
    <dbReference type="eggNOG" id="arCOG04740">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099871_0_0_2"/>
    <dbReference type="OrthoDB" id="284254at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054383">
        <property type="entry name" value="PspAB-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22742">
        <property type="entry name" value="PspAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="199" mass="22188" checksum="3314538BA7F11229" modified="2017-05-10" version="1">MGIFETVRQVLGLSAESGATRDADPEDLFGMSTAYLTMEAELGFAPTGDAALCFADVDSTAFREAREEVEAVLELGEADGTTADFIEDSHGYHWVVLHDTDFENLVSSIHFAADTLIEEGFGSRLLAALFAFERDGTTAYWVYSFRRGAYYPFVPTGGRQRDQKLEFKLESSFDGELAIEDDTSYWYPMWPEGDTHPWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUH4</accession>
    <name>A0A1U7EUH4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Molybdopterin adenylyltransferase</fullName>
            <ecNumber evidence="4">2.7.7.75</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">moaB2</name>
        <name evidence="4" type="ordered locus">NP_1040A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MoaB/Mog family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.75" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322246.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUH4"/>
    <dbReference type="STRING" id="348780.NP_1040A"/>
    <dbReference type="EnsemblBacteria" id="CAI48611">
        <property type="protein sequence ID" value="CAI48611"/>
        <property type="gene ID" value="NP_1040A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702924"/>
    <dbReference type="KEGG" id="nph:NP_1040A"/>
    <dbReference type="eggNOG" id="arCOG00214">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077358_2_1_2"/>
    <dbReference type="OrthoDB" id="205337at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061598">
        <property type="term" value="F:molybdopterin adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00886">
        <property type="entry name" value="MogA_MoaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012245">
        <property type="entry name" value="MoaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008284">
        <property type="entry name" value="MoCF_biosynth_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43232">
        <property type="entry name" value="MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43232:SF2">
        <property type="entry name" value="MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006443">
        <property type="entry name" value="MoaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01078">
        <property type="entry name" value="MOCF_BIOSYNTHESIS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MoaB/Mog" evidence="3">
        <location>
            <begin position="28"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="182" mass="19165" checksum="1FA707A3617189D3" modified="2017-05-10" version="1">MTDKRRPPADRRGTDDDGHAVVDPLYVAVITVSSSRDVDDDPGGDAAVDCVEAAGGRVTARRAISDDFAAIRGLVSRLAAREDVDCIVTTGGTGMTMDDVTPTACEGLFEREIPGFGELFRQLSYDEIGHRAMASRAIAGIVAGVPVFCLPGSRAAVETGLTELVLPEAPHLAGLATRHQFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUK0</accession>
    <name>A0A1U7EUK0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7123 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1130A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322292.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUK0"/>
    <dbReference type="STRING" id="348780.NP_1130A"/>
    <dbReference type="EnsemblBacteria" id="CAI48656">
        <property type="protein sequence ID" value="CAI48656"/>
        <property type="gene ID" value="NP_1130A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701059"/>
    <dbReference type="KEGG" id="nph:NP_1130A"/>
    <dbReference type="eggNOG" id="arCOG02867">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_172273_0_0_2"/>
    <dbReference type="OrthoDB" id="186577at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055547">
        <property type="entry name" value="DUF7123"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23438">
        <property type="entry name" value="DUF7123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7123" evidence="1">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="84" mass="9184" checksum="A68D33354AA6F868" modified="2017-05-10" version="1">MSATAAAATADLTRKQRRILTYLREKGQTKTYFKSRLIGDALDMSAKEVGANMTAIQESDVDVSVEKWGYSSGTTWKVDVYGGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUK1</accession>
    <name>A0A1U7EUK1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF8134 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_1148A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322301.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUK1"/>
    <dbReference type="STRING" id="348780.NP_1148A"/>
    <dbReference type="EnsemblBacteria" id="CAI48665">
        <property type="protein sequence ID" value="CAI48665"/>
        <property type="gene ID" value="NP_1148A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701066"/>
    <dbReference type="KEGG" id="nph:NP_1148A"/>
    <dbReference type="eggNOG" id="arCOG04747">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_163798_0_0_2"/>
    <dbReference type="OrthoDB" id="193938at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058447">
        <property type="entry name" value="DUF8134"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26455">
        <property type="entry name" value="DUF8134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8134" evidence="2">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="113" mass="12523" checksum="F1136F87CBDBADBD" modified="2017-05-10" version="1">MPTSVHQLDDGAWLSVNDSREVNVSDLWWLARHEFCDCAMADFLAEGFVEIGVDYPNVEGRIAGQCIACGESGVTDWLTLGRVVDPEEGTFSPVDTESVHIPRRRQRLADASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUL6</accession>
    <name>A0A1U7EUL6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Aminopeptidase (Homolog to leucyl aminopeptidase / aminopeptidase T)</fullName>
            <ecNumber evidence="10">3.4.11.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">pepB3</name>
        <name evidence="10" type="ordered locus">NP_1140A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase M29 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-" evidence="10"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322297.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUL6"/>
    <dbReference type="STRING" id="348780.NP_1140A"/>
    <dbReference type="EnsemblBacteria" id="CAI48661">
        <property type="protein sequence ID" value="CAI48661"/>
        <property type="gene ID" value="NP_1140A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703013"/>
    <dbReference type="KEGG" id="nph:NP_1140A"/>
    <dbReference type="eggNOG" id="arCOG01889">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057697_0_0_2"/>
    <dbReference type="OrthoDB" id="145069at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1830.10">
        <property type="entry name" value="Thermophilic metalloprotease (M29)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052170">
        <property type="entry name" value="M29_Exopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035097">
        <property type="entry name" value="M29_N-terminal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000787">
        <property type="entry name" value="Peptidase_M29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34448">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34448:SF1">
        <property type="entry name" value="BLL6088 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02073">
        <property type="entry name" value="Peptidase_M29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144052">
        <property type="entry name" value="Thermophilic metalloprotease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="8 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI48661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="374" mass="40767" checksum="7BEFA5AC2D4445FD" modified="2017-05-10" version="1">MDERVREHAAVLVDWSARVDPGDDVVISVAEDAHELAVAVAERLGEVGANVVTTYGSAELQRAYLQAHDGDFEAGDHEAALLSAADVYLRLGGGRNTAATADVESETRQAYSRARANIRDARMDTDWVSTVHPTRSLAQQAGMAYEAYQEFVYNAVLRDWEALAERMATVKALLDDGERVRIVKERDDQPRTDIQLSIADRTAVNSAASVAYDSHNLPSGEVFTAPADADGEVFFDVPMTISDRRVRDVRLVFEAGEVVDYEAAQGESVIESVLETDAGARRLGELGIGMNRGIDRVTDNILFDEKMGETAHLALGRAYDANLPDGESGNDSAVHVDLITDLSEASRIEVDGDVVQRNGVFYFEDGFEAAKRPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUM1</accession>
    <name>A0A1U7EUM1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Rhomboid family protease</fullName>
            <ecNumber evidence="13">3.4.21.105</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">NP_1156A</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S54 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.105" evidence="13"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322305.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUM1"/>
    <dbReference type="STRING" id="348780.NP_1156A"/>
    <dbReference type="EnsemblBacteria" id="CAI48669">
        <property type="protein sequence ID" value="CAI48669"/>
        <property type="gene ID" value="NP_1156A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701070"/>
    <dbReference type="KEGG" id="nph:NP_1156A"/>
    <dbReference type="eggNOG" id="arCOG01769">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055068_1_0_2"/>
    <dbReference type="OrthoDB" id="26567at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1110.10">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035896">
        <property type="entry name" value="AN1-like_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050925">
        <property type="entry name" value="Rhomboid_protease_S54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000058">
        <property type="entry name" value="Znf_AN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43731:SF14">
        <property type="entry name" value="PRESENILIN-ASSOCIATED RHOMBOID-LIKE PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43731">
        <property type="entry name" value="RHOMBOID PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01428">
        <property type="entry name" value="zf-AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00154">
        <property type="entry name" value="ZnF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118310">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51039">
        <property type="entry name" value="ZF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="92"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="141"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="206"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="231"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="262"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="AN1-type" evidence="12">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="292"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="300"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAI48669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="314" mass="33315" checksum="B75F4A0C85C4AE7E" modified="2017-05-10" version="1">MATCDACGEHENMPYNCRHCGGTFCADHRLPENHNCPGLESWNDPDKVFDSGFDGSVQAGGSGGRSPGLLERLGIDTGPGGPLAYFRNNVSYLFLAIIVVVFGLQWLIAPLFMALPPEGVNPGHFLWGQIFTLTTVHPEYVWTWVISVFSHGSPMHLLFNAIVLYFFGPLVERQIGSKKFVGLFLASGIIAGLGQVGVGLVTSEGVAVLGASGALMAIMGVLAITSPDLKVLLFFFIPMSIRTLTILFAAFSIFAFVSDGGILDGVAHFAHLVGLLIGLWYGNRIKDRVGGGPRQLNLGPGRGGGGPGGPGRFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUN0</accession>
    <name>A0A1U7EUN0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC-type transport system ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">abc26a</name>
        <name evidence="6" type="ordered locus">NP_1198A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
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        <property type="protein sequence ID" value="CAI48690.1"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUN0"/>
    <dbReference type="STRING" id="348780.NP_1198A"/>
    <dbReference type="EnsemblBacteria" id="CAI48690">
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        <property type="gene ID" value="NP_1198A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702445"/>
    <dbReference type="KEGG" id="nph:NP_1198A"/>
    <dbReference type="eggNOG" id="arCOG00194">
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    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
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    <dbReference type="Proteomes" id="UP000002698">
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    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03230">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="4"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI48690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="302" mass="31632" checksum="57B0BA02EE2AABDC" modified="2017-05-10" version="1">MPALQLESVHKRFGDVVALDDVSFSVPDGEVFGFLGPNGAGKSTTIDIVLDYVRPTDGSVSVFGHDAQADPTAVHARTGVLPDATALAGHLTATEHVRFAIDSKGADDDTDALLERVGLDGVGDRRVGGFSKGMRQRLLLATALVGEPELLILDEPSTGLDPNGARRMREIVREENDRGATVLFSSHILEQVEAVSDRVGILADGELVACDSIDGLRGRLGSKARLSVTVDELSDAAIEAVEALDGVVDVSVTGRTVAAACPDDRKAAVVDAVRTDGATVEDIETGEASLEELFAAYTGESQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUQ8</accession>
    <name>A0A1U7EUQ8_NATPD</name>
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        <submittedName>
            <fullName evidence="2">DUF460 domain protein</fullName>
        </submittedName>
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        <name evidence="2" type="ordered locus">NP_1264A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUQ8"/>
    <dbReference type="STRING" id="348780.NP_1264A"/>
    <dbReference type="EnsemblBacteria" id="CAI48723">
        <property type="protein sequence ID" value="CAI48723"/>
        <property type="gene ID" value="NP_1264A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701097"/>
    <dbReference type="KEGG" id="nph:NP_1264A"/>
    <dbReference type="eggNOG" id="arCOG04219">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027052_1_0_2"/>
    <dbReference type="OrthoDB" id="15228at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007408">
        <property type="entry name" value="DUF460"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40707:SF1">
        <property type="entry name" value="DUF460 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40707">
        <property type="entry name" value="POSSIBLE NUCLEASE OF RNASE H FOLD, RUVC/YQGF FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04312">
        <property type="entry name" value="DUF460"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="394"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="635"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="396"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="406"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="657" mass="73876" checksum="47D5A2728EF2DE5C" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUR7</accession>
    <name>A0A1U7EUR7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1262A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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            <authorList>
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUR7"/>
    <dbReference type="EnsemblBacteria" id="CAI48722">
        <property type="protein sequence ID" value="CAI48722"/>
        <property type="gene ID" value="NP_1262A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701096"/>
    <dbReference type="KEGG" id="nph:NP_1262A"/>
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    <dbReference type="Proteomes" id="UP000002698">
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    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF24282">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="69" mass="7272" checksum="DF223EE8B3D5F023" modified="2017-05-10" version="1">MNQEVIKKSLGETGQAGVVALVAGLVILGLRDRKAAFGALVVVVGIALVGYGLGDRFLKSMGMEYEDLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUU7</accession>
    <name>A0A1U7EUU7_NATPD</name>
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        <submittedName>
            <fullName evidence="1">CopG domain protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ordered locus">NP_1352A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
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            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="STRING" id="348780.NP_1352A"/>
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        <property type="protein sequence ID" value="CAI48767"/>
        <property type="gene ID" value="NP_1352A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701123"/>
    <dbReference type="KEGG" id="nph:NP_1352A"/>
    <dbReference type="eggNOG" id="arCOG04735">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_197985_0_0_2"/>
    <dbReference type="OrthoDB" id="334822at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="RHH_NikR_HicB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="entry name" value="Met repressor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36215">
        <property type="entry name" value="BLL4998 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36215:SF2">
        <property type="entry name" value="RIBBON-HELIX-HELIX PROTEIN COPG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="Ribbon-helix-helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="59" mass="6747" checksum="CCD115BE98A2D605" modified="2017-05-10" version="1">MPKISVEIPQELLADLDEHVGDDGKFVNRSDAVRASIRKTLDMLDEIDARHDRLDENDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV00</accession>
    <name>A0A1U7EV00_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">SufB domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">sufB2</name>
        <name evidence="4" type="synonym">abc31p2</name>
        <name evidence="4" type="ordered locus">NP_1500A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
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            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the iron-sulfur cluster assembly SufBD family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322475.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A1U7EV00"/>
    <dbReference type="STRING" id="348780.NP_1500A"/>
    <dbReference type="EnsemblBacteria" id="CAI48841">
        <property type="protein sequence ID" value="CAI48841"/>
        <property type="gene ID" value="NP_1500A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702458"/>
    <dbReference type="KEGG" id="nph:NP_1500A"/>
    <dbReference type="eggNOG" id="arCOG01715">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026231_3_1_2"/>
    <dbReference type="OrthoDB" id="300624at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055346">
        <property type="entry name" value="Fe-S_cluster_assembly_SufBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000825">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037284">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011542">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045595">
        <property type="entry name" value="SufBD_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01981">
        <property type="entry name" value="sufD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30508">
        <property type="entry name" value="FES CLUSTER ASSEMBLY PROTEIN SUF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30508:SF1">
        <property type="entry name" value="UPF0051 PROTEIN ABCI8, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01458">
        <property type="entry name" value="SUFBD_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19295">
        <property type="entry name" value="SufBD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101960">
        <property type="entry name" value="Stabilizer of iron transporter SufD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUF system FeS cluster assembly SufBD N-terminal" evidence="3">
        <location>
            <begin position="60"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="SUF system FeS cluster assembly SufBD core" evidence="2">
        <location>
            <begin position="146"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00043967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="405" mass="45129" checksum="EB9E4D1226DFE6E9" modified="2017-05-10" version="1">MSTQVHASIDEATVRQISDELDEPEWLLETRLEALEALEELPFPDVIQTPGRKWTNLEDLDYEVLVDPLDAAEEKDQVGPDTVDVLPFAEAVDEHGDLLEEHFGSVVDPETNYLTALSTALFTTGTVVYVPEGTDAEDVTIRTKMNSRSLFNYTLVVAEESSSVTILERQESGDEVDGNRYYSGLTEVVAGENSYVQFGNLQDLDQTTYTYSLKEAAIDTYGNVNWIECNIGSKLTKSSVESYLDGDSSESKIVGAFYGHDDQHLDIDARVWHTAEHTTADLVTRGVIDDEARSVYEGVQDVGADAWDTNSYQRENTLMLSDESEADASPKLIINNHDTEASHSATVGQVSAEEMFYMTSRALPERLAKNMLVEGFYVPVLEEIEVEELREELKGRVRGRLQARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV28</accession>
    <name>A0A1U7EV28_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">NP_1506A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48844.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV28"/>
    <dbReference type="STRING" id="348780.NP_1506A"/>
    <dbReference type="EnsemblBacteria" id="CAI48844">
        <property type="protein sequence ID" value="CAI48844"/>
        <property type="gene ID" value="NP_1506A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_1506A"/>
    <dbReference type="eggNOG" id="arCOG02327">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_58_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00075">
        <property type="entry name" value="HATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031621">
        <property type="entry name" value="HisKA_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050736">
        <property type="entry name" value="Sensor_HK_Regulatory"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43711:SF1">
        <property type="entry name" value="HISTIDINE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43711">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16927">
        <property type="entry name" value="HisKA_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="72"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="210"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="9">
        <location>
            <begin position="242"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="10">
        <location>
            <begin position="298"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="8">
        <location>
            <begin position="371"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="341"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI48844.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="580" mass="64574" checksum="202F8EB94834A4D1" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV59</accession>
    <name>A0A1U7EV59_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thioredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">trxA6</name>
        <name evidence="2" type="ordered locus">NP_1606A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322528.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV59"/>
    <dbReference type="STRING" id="348780.NP_1606A"/>
    <dbReference type="EnsemblBacteria" id="CAI48894">
        <property type="protein sequence ID" value="CAI48894"/>
        <property type="gene ID" value="NP_1606A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703430"/>
    <dbReference type="KEGG" id="nph:NP_1606A"/>
    <dbReference type="eggNOG" id="arCOG06181">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090892_1_0_2"/>
    <dbReference type="OrthoDB" id="115386at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02966">
        <property type="entry name" value="TlpA_like_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013740">
        <property type="entry name" value="Redoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050553">
        <property type="entry name" value="Thioredoxin_ResA/DsbE_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42852">
        <property type="entry name" value="THIOL:DISULFIDE INTERCHANGE PROTEIN DSBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42852:SF17">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN HI_1115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08534">
        <property type="entry name" value="Redoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="1">
        <location>
            <begin position="15"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="174" mass="18363" checksum="A9E4B05482A14DF8" modified="2017-05-10" version="1">MRRRDVLIGAGSLAVLGGGAAVAFDAVGSDSGTYVDPVDLETLDAPGSEAGTTTVPDPGRVTFVELFAKWCGICEDMMPALAEASAAVEGVQFVSVTNEALGTTTTREDVIEWWRDHDGAWPVAVDADLALTQRLDATGVPYAFVIDAENRVSWQHRGASDAEEIIAQIRAVQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV74</accession>
    <name>A0A1U7EV74_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1626A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322538.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV74"/>
    <dbReference type="STRING" id="348780.NP_1626A"/>
    <dbReference type="EnsemblBacteria" id="CAI48904">
        <property type="protein sequence ID" value="CAI48904"/>
        <property type="gene ID" value="NP_1626A"/>
    </dbReference>
    <dbReference type="GeneID" id="41345081"/>
    <dbReference type="KEGG" id="nph:NP_1626A"/>
    <dbReference type="eggNOG" id="arCOG15105">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_195003_0_0_2"/>
    <dbReference type="OrthoDB" id="226414at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="67" mass="7339" checksum="0DEC0D14BB47DB86" modified="2017-05-10" version="1">MARVPITEPVVEQLRDIISDGVLDDEHNYMGAQFAAQDRGHEELAAFVSTADAATYYEALQQAKAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV77</accession>
    <name>A0A1U7EV77_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_1590A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48886.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV77"/>
    <dbReference type="STRING" id="348780.NP_1590A"/>
    <dbReference type="EnsemblBacteria" id="CAI48886">
        <property type="protein sequence ID" value="CAI48886"/>
        <property type="gene ID" value="NP_1590A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_1590A"/>
    <dbReference type="eggNOG" id="arCOG04644">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_709049_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041346">
        <property type="entry name" value="DR2241_Fer4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041181">
        <property type="entry name" value="DR2241_middle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055772">
        <property type="entry name" value="DUF7348"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18069">
        <property type="entry name" value="DR2241"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24039">
        <property type="entry name" value="DUF7348"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18009">
        <property type="entry name" value="Fer4_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7348" evidence="3">
        <location>
            <begin position="5"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="DR2241 stabilising" evidence="2">
        <location>
            <begin position="92"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="DR2241 4Fe-4S iron-sulfur cluster binding" evidence="1">
        <location>
            <begin position="205"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48886.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="364" mass="41351" checksum="7A6918F5E9B20230" modified="2017-05-10" version="1">MTDVNDDQLAALVAAADDGVDCDGLVVERTEAGYRFETPGTTHEELSEAAFRDVAAENPWFVSNWYYWRARDRPESEVSFLRWLESADDRSVPERYEGFAADGLNASWGQLHIDIGLGDDGHRVYTLRNEADASADTEALAVHTDPLDARHLVKHDNDGRFRPLKTAPTLPTGWAFVDLDGRALAETVEYIYPATIANWHLERQGDLDVSHWRETMERQSGIYSVIKTWDRGEGYEHVNWVAEACCDDSQCLKRREWQYDDETDLDVDGGDGEFPCREPCSLVVTAARKWTKLEGEQSQTYEFELTPSEKEQVEEIIDAVADGEADDIREADIYEGANRYRARFLRAKRFDDDGRFCGVPTDDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV96</accession>
    <name>A0A1U7EV96_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hydroxymethylglutaryl-CoA reductase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1690A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322569.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV96"/>
    <dbReference type="STRING" id="348780.NP_1690A"/>
    <dbReference type="EnsemblBacteria" id="CAI48936">
        <property type="protein sequence ID" value="CAI48936"/>
        <property type="gene ID" value="NP_1690A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701225"/>
    <dbReference type="KEGG" id="nph:NP_1690A"/>
    <dbReference type="eggNOG" id="arCOG05512">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_968444_0_0_2"/>
    <dbReference type="OrthoDB" id="238651at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057868">
        <property type="entry name" value="HMG-CoA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25653">
        <property type="entry name" value="HMG-CoA_red_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydroxymethylglutaryl-CoA reductase-like" evidence="1">
        <location>
            <begin position="6"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="287" mass="32157" checksum="ABF34B963CA1CC80" modified="2017-05-10" version="1">MIEQYLERLFTWESLKNTERGVEFELKNRLIEAEFQGVTSAKVDGEPVPPERITLELSESERYQADEISAENPMYLGLAETVQVILDRQRLHLGVHEIELGLQIEGYGVVGFVTDDEIREEDLLDVDPGDYTVDELETLVGAITEPVTLERLLEREQEGKARTTAINCIEARLEVTEPLTKEEMVRAEPTTNAGGTIVGELLVELLESPQRVSVYLTAQALGGATAEEIASKTVFPESVVESTLADFEREGIAERTDDEEETYTVADPISVLQKRQRDLWTALRAAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVA1</accession>
    <name>A0A1U7EVA1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transducer protein Htr29</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">htr29</name>
        <name evidence="9" type="ordered locus">NP_1720A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322584.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVA1"/>
    <dbReference type="STRING" id="348780.NP_1720A"/>
    <dbReference type="EnsemblBacteria" id="CAI48951">
        <property type="protein sequence ID" value="CAI48951"/>
        <property type="gene ID" value="NP_1720A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702843"/>
    <dbReference type="KEGG" id="nph:NP_1720A"/>
    <dbReference type="eggNOG" id="arCOG02318">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_116_0_2"/>
    <dbReference type="OrthoDB" id="116658at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11386">
        <property type="entry name" value="MCP_signal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.950">
        <property type="entry name" value="Methyl-accepting chemotaxis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004090">
        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32089:SF112">
        <property type="entry name" value="LYSOZYME-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32089">
        <property type="entry name" value="METHYL-ACCEPTING CHEMOTAXIS PROTEIN MCPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00260">
        <property type="entry name" value="CHEMTRNSDUCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58104">
        <property type="entry name" value="Methyl-accepting chemotaxis protein (MCP) signaling domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="PAS" evidence="7">
        <location>
            <begin position="63"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="8">
        <location>
            <begin position="202"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="6">
        <location>
            <begin position="252"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="10"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="267"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="330"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="393"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="477"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI48951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="534" mass="57488" checksum="9C60748B01691CF3" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVD9</accession>
    <name>A0A1U7EVD9_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9 10">DNA repair and recombination protein RadB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 12" type="primary">radB</name>
        <name evidence="12" type="ordered locus">NP_1788A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Involved in DNA repair and in homologous recombination. May regulate the cleavage reactions of the branch-structured DNA. Has a very weak ATPase activity that is not stimulated by DNA. Binds DNA but does not promote DNA strands exchange.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the eukaryotic RecA-like protein family. RadB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322618.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVD9"/>
    <dbReference type="STRING" id="348780.NP_1788A"/>
    <dbReference type="EnsemblBacteria" id="CAI48985">
        <property type="protein sequence ID" value="CAI48985"/>
        <property type="gene ID" value="NP_1788A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703096"/>
    <dbReference type="KEGG" id="nph:NP_1788A"/>
    <dbReference type="eggNOG" id="arCOG00417">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041732_2_0_2"/>
    <dbReference type="OrthoDB" id="17644at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00350">
        <property type="entry name" value="RadB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011939">
        <property type="entry name" value="DNA_repair_and_recomb_RadB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006861">
        <property type="entry name" value="PRK09361.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02237">
        <property type="entry name" value="recomb_radB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942:SF47">
        <property type="entry name" value="DNA REPAIR AND RECOMBINATION PROTEIN RADB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942">
        <property type="entry name" value="RECA/RAD51/RADA DNA STRAND-PAIRING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003336">
        <property type="entry name" value="RadB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01874">
        <property type="entry name" value="DNAREPAIRADA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecA family profile 1" evidence="11">
        <location>
            <begin position="1"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="NCBIfam" id="TIGR02237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI48985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="231" mass="24600" checksum="07E6A82AAB2832D6" modified="2017-05-10" version="1">MSEPIPTGCDTVDTLLGGGFERGTVTQLYGPPASGKTNLALSAAVQVAADGGTALYIDTEGVSLDRFEQLARGATDDLEGTTSRVIISDALDFDDQREAVQDAGDLAATADLVVLDSATGFYRLERDTDDDGETLREVATQVTHLLGLARRHDLAVVITNQVYTDPEADSSRVRPLGGHTLSHWSGTVLRIERFRGGNRRATLEKHQAKAAGETARFKITDSGLEGVDEGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVE4</accession>
    <name>A0A1U7EVE4_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glutamate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">gdhA1</name>
        <name evidence="10" type="ordered locus">NP_1806A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homohexamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 7">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322626.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVE4"/>
    <dbReference type="STRING" id="348780.NP_1806A"/>
    <dbReference type="EnsemblBacteria" id="CAI48994">
        <property type="protein sequence ID" value="CAI48994"/>
        <property type="gene ID" value="NP_1806A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702748"/>
    <dbReference type="KEGG" id="nph:NP_1806A"/>
    <dbReference type="eggNOG" id="arCOG01352">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025763_1_2_2"/>
    <dbReference type="OrthoDB" id="6425at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004352">
        <property type="term" value="F:glutamate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006538">
        <property type="term" value="P:L-glutamate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01076">
        <property type="entry name" value="NAD_bind_1_Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000003">
        <property type="entry name" value="Glutamate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006095">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006097">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033524">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014362">
        <property type="entry name" value="Glu_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054867">
        <property type="entry name" value="GluDhGdhB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033922">
        <property type="entry name" value="NAD_bind_Glu_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041398">
        <property type="entry name" value="GluDhGdhB_Halo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606:SF13">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02812">
        <property type="entry name" value="ELFV_dehydrog_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000185">
        <property type="entry name" value="Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00082">
        <property type="entry name" value="GLFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00074">
        <property type="entry name" value="GLFV_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal" evidence="9">
        <location>
            <begin position="192"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalysis" evidence="6">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI48994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="424" mass="45863" checksum="8BF839C09AF3A1D1" modified="2017-05-10" version="1">MPGAAGDDEAVETETALETARLQLDRAAAHLDIDPNVVERLHHPRRVQEVTVPIERDDGSVEAFTGYRAQHDSVRGPYKGGLRYHPEVTHDECVGLAMWMTWKCAVMDLPFGGAKGGVAVDPKSLSDDEKERLTRRFAQEIRGVIGPMQDIPAPDMGTDPQTMAWLMDAYSMQEGETTPGVVTGKPPVIGGSKGREEAPGRSVAIITRAVCEYYDRPLSETTIAIQGYGSVGANAARLLDEWGATIVAVSDVNGAMYAPDGIDTASVPSHDEEPEAVTEYADTVISNDELLELDVDVLVPAALGNVITADNATDISADIVVEGANGPTTTTGDAILEERGIRVIPDILANAGGVTVSYFEWLQDINRRAWSLERVNEELETEMLDAWDAVTDAHETYDVSWRDAAYIVALKRVAAAHEARGLWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVG9</accession>
    <name>A0A1U7EVG9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">5'-nucleotidase family hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ushA1</name>
        <name evidence="4" type="ordered locus">NP_1854A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322650.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVG9"/>
    <dbReference type="STRING" id="348780.NP_1854A"/>
    <dbReference type="EnsemblBacteria" id="CAI49018">
        <property type="protein sequence ID" value="CAI49018"/>
        <property type="gene ID" value="NP_1854A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703455"/>
    <dbReference type="KEGG" id="nph:NP_1854A"/>
    <dbReference type="eggNOG" id="arCOG02832">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005854_7_1_2"/>
    <dbReference type="OrthoDB" id="21342at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009166">
        <property type="term" value="P:nucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00845">
        <property type="entry name" value="MPP_UshA_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.780.10">
        <property type="entry name" value="5'-Nucleotidase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008334">
        <property type="entry name" value="5'-Nucleotdase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036907">
        <property type="entry name" value="5'-Nucleotdase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006146">
        <property type="entry name" value="5'-Nucleotdase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006179">
        <property type="entry name" value="5_nucleotidase/apyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11575:SF24">
        <property type="entry name" value="5'-NUCLEOTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11575">
        <property type="entry name" value="5'-NUCLEOTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02872">
        <property type="entry name" value="5_nucleotid_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="APYRASEFAMLY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55816">
        <property type="entry name" value="5'-nucleotidase (syn. UDP-sugar hydrolase), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00786">
        <property type="entry name" value="5_NUCLEOTIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="2">
        <location>
            <begin position="36"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="5'-Nucleotidase C-terminal" evidence="3">
        <location>
            <begin position="368"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="651" mass="70288" checksum="222BA1782E6D42FC" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVN5</accession>
    <name>A0A1U7EVN5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Molybdopterin-binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_2044A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322742.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVN5"/>
    <dbReference type="STRING" id="348780.NP_2044A"/>
    <dbReference type="EnsemblBacteria" id="CAI49113">
        <property type="protein sequence ID" value="CAI49113"/>
        <property type="gene ID" value="NP_2044A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701334"/>
    <dbReference type="KEGG" id="nph:NP_2044A"/>
    <dbReference type="eggNOG" id="arCOG00266">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003827_2_1_2"/>
    <dbReference type="OrthoDB" id="9576at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008482">
        <property type="term" value="F:sulfite oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006790">
        <property type="term" value="P:sulfur compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.420.10">
        <property type="entry name" value="Oxidoreductase, molybdopterin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005066">
        <property type="entry name" value="MoCF_OxRdtse_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000572">
        <property type="entry name" value="OxRdtase_Mopterin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036374">
        <property type="entry name" value="OxRdtase_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19372:SF7">
        <property type="entry name" value="SULFITE OXIDASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19372">
        <property type="entry name" value="SULFITE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03404">
        <property type="entry name" value="Mo-co_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00174">
        <property type="entry name" value="Oxidored_molyb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56524">
        <property type="entry name" value="Oxidoreductase molybdopterin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Oxidoreductase molybdopterin-binding" evidence="2">
        <location>
            <begin position="245"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Moybdenum cofactor oxidoreductase dimerisation" evidence="3">
        <location>
            <begin position="409"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="508" mass="55498" checksum="602C5DA69D366FEF" modified="2017-05-10" version="1">MSDSDSARSDNGSAIGRYGMAAIAVVAAGVSTVATSFAVYATGREFLVSSLNRHLLEYLPGWFVSGVIQRFGNLALELSMVFSGALLAVVFGIVAVGGYRLGDRLSPTLPPVAGFVVATALVFVVSYSVVGSAMAVVVPALVGGVVVTYFETRSPTEIATDSERRSVLRAVSAVAAFNVVAHGIGLVRRSQTQRAEQELQERALRLEAEHMISDARQKELDVEGVQPLVTDIEDFYKVDINPSPPAVDPDSWALSITGAVEEPVDLDYAEIQRFPVEHQYKAIRCLSDDIEDDKLDTAVWTGCSMDAVLDAVDLQGSHVMLYGADDYFYSMSVEDFRSCLLAYGMNGLELPQAHGYPVRLLVPDRWGKLHVKWLTEIEVIDDYSGGYWEEQGWHGMGAVNAVTKIDRINRPEGRIQICGHAYAGARGVDRVEVSIDGGETWTAAALSGQLDNPDTIRQWVYEIDDPQQDEYEVYARTIDGTDTIQTEVRTDPFPDGATGWVNRTIRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVR6</accession>
    <name>A0A1U7EVR6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF95 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2108A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322773.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVR6"/>
    <dbReference type="STRING" id="348780.NP_2108A"/>
    <dbReference type="EnsemblBacteria" id="CAI49145">
        <property type="protein sequence ID" value="CAI49145"/>
        <property type="gene ID" value="NP_2108A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701350"/>
    <dbReference type="KEGG" id="nph:NP_2108A"/>
    <dbReference type="eggNOG" id="arCOG01994">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044678_0_0_2"/>
    <dbReference type="OrthoDB" id="86288at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002798">
        <property type="entry name" value="SpoIIM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35337">
        <property type="entry name" value="SLR1478 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35337:SF1">
        <property type="entry name" value="SLR1478 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01944">
        <property type="entry name" value="SpoIIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="262"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="391"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="431"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="471"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="499" mass="50652" checksum="33A4CEFDDD8C3F8B" modified="2017-05-10" version="1">MAIAPALRAVIAVFRGRASDLLPAFFLTPAISAVVQVVGLGGFALSYLYLATTGRLERFQSALADRELDPPSPEDPAFEAWLEELVPVFEVLLPPAVVGLLAVTALVAVVLFVALYGVALAAQLSASVATLRDRRGTTAALAGARRHTGAMVVLVVFEVFLWVLATAVAGVAGAMAALLLPGILFVAVALVVTLAWLAAVVCIRALFAFAPVAVVEDGVGAGDGLRRAGGYIRRNAGDAVGYYLLALGLLGVVGVLSGGGEAGARAAAVAGFVVVSPALAVLKTALYAGTVDAIAPPAVPERSAAAQLSSGLRRGLAELGAFVRATPGTHAVATGLILGGFGLGWMLAAPYEGLVTASIEARLEDHFAPTAAVFFAANNWTVAVGTALSGLTLAVPAAASLVFNGVVFGVYGRLEADPEALAAFVAPHGVLEIPAIIAAGAAGLWLGVAGWRRLRGRCSDAELADRIERSFWVLVGIGVVLAVAGFIEGFISPSVAGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVS7</accession>
    <name>A0A1U7EVS7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Creatininase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cre2</name>
        <name evidence="5" type="ordered locus">NP_2120A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322779.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVS7"/>
    <dbReference type="STRING" id="348780.NP_2120A"/>
    <dbReference type="EnsemblBacteria" id="CAI49151">
        <property type="protein sequence ID" value="CAI49151"/>
        <property type="gene ID" value="NP_2120A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701355"/>
    <dbReference type="KEGG" id="nph:NP_2120A"/>
    <dbReference type="eggNOG" id="arCOG04536">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055029_2_1_2"/>
    <dbReference type="OrthoDB" id="46121at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10310">
        <property type="entry name" value="Creatininase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024087">
        <property type="entry name" value="Creatininase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003785">
        <property type="entry name" value="Creatininase/forma_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35005:SF1">
        <property type="entry name" value="2-AMINO-5-FORMYLAMINO-6-RIBOSYLAMINOPYRIMIDIN-4(3H)-ONE 5'-MONOPHOSPHATE DEFORMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35005">
        <property type="entry name" value="3-DEHYDRO-SCYLLO-INOSOSE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02633">
        <property type="entry name" value="Creatininase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102215">
        <property type="entry name" value="Creatininase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="263" mass="28955" checksum="7D59BA95E32A848E" modified="2017-05-10" version="1">MYLGDVSWPEAGTHLDATDVALVPVGSTEQHGPHLPLSTDHVIAEGLAREAADRAGVVCTPTITVGVSPHHRQFHGTMSVEAPVFRDYVESLSRSLVYQGIDRIVYVNAHGGNVEHLREVGRRLHEDGAAYAVEWMWDESIPDLVDDLFEQNGPHAGPKETSMVWYLADNVREDELADARDGGCPALEDIRTTYNGARLFYDAIENSDNGAFGDPTDATAAKGERLFEAASEQLADLAEWLAAQPWETLAPREHVRSRDESKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVX8</accession>
    <name>A0A1U7EVX8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ArNOG05511 family protein (AAA-type ATPase core domain protein)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">NP_2252A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322844.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVX8"/>
    <dbReference type="STRING" id="348780.NP_2252A"/>
    <dbReference type="EnsemblBacteria" id="CAI49217">
        <property type="protein sequence ID" value="CAI49217"/>
        <property type="gene ID" value="NP_2252A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701392"/>
    <dbReference type="KEGG" id="nph:NP_2252A"/>
    <dbReference type="eggNOG" id="arCOG04368">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_646568_0_0_2"/>
    <dbReference type="OrthoDB" id="45425at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19481">
        <property type="entry name" value="RecA-like_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="204"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI49217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="442" mass="50120" checksum="A63619A91F3BC246" modified="2017-05-10" version="1">MSSPELDVVEFLLTAAIYTDNRDLDERDLPPRYRRVFWEDGEVERPLTTTTNTAAAATGVDRPWEAISGLMFTDRDDFSGKIEFTDREMAESWFLERVSVEALRDNPTLAHAFGDQVDAEVDYETARSKNRPIHADRVWIDSLLEEMFDEDEEEMLDLVEIRAPEEIETTLDDLVLTTDQENEIHKIVKAIEHRDYLAEIGLREIGKLLFVGPPGTGKTSVARALAQDLDLPFVEVKLSMITSQYLGETAKNVDKTFEVAKRLSPCILFMDEFDFVAKTRSSDEHAAIKRAVNTLLKSIDEISLIQDDVLLIGATNHPDQLDAAAWRRFDEIVNFPKPDRDMRADILRIVTRRMDVDDFDPEALAEATEGLTGSDLRLVLREAVLDALTEERTTLTQDDLLEAIEGFEERDNLKNMDMIEGDADALIADGSDGHDHDHDHDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVY8</accession>
    <name>A0A1U7EVY8_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thiamine-monophosphate kinase</fullName>
            <shortName evidence="1">TMP kinase</shortName>
            <shortName evidence="1">Thiamine-phosphate kinase</shortName>
            <ecNumber evidence="1">2.7.4.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1 4" type="primary">thiL</name>
        <name evidence="4" type="ordered locus">NP_2280A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent phosphorylation of thiamine-monophosphate (TMP) to form thiamine-pyrophosphate (TPP), the active form of vitamin B1.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>thiamine phosphate + ATP = thiamine diphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:15913"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine diphosphate from thiamine phosphate: step 1/1.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Reaction mechanism of ThiL seems to utilize a direct, inline transfer of the gamma-phosphate of ATP to TMP rather than a phosphorylated enzyme intermediate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiamine-monophosphate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.16" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322858.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVY8"/>
    <dbReference type="STRING" id="348780.NP_2280A"/>
    <dbReference type="EnsemblBacteria" id="CAI49231">
        <property type="protein sequence ID" value="CAI49231"/>
        <property type="gene ID" value="NP_2280A"/>
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    <dbReference type="KEGG" id="nph:NP_2280A"/>
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        <property type="reaction ID" value="UER00142"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:thiamine-phosphate kinase activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    </dbReference>
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        <location>
            <begin position="18"/>
            <end position="123"/>
        </location>
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            <begin position="135"/>
            <end position="274"/>
        </location>
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        <location>
            <position position="20"/>
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        <ligand>
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        <ligand>
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        </ligand>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI49231.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="289" mass="29682" checksum="31DF6CDC1E60A922" modified="2017-05-10" version="1">MDERAALSLVGDRVAAAGDDCAVVDDLVVTTDMLHETTDFPDGTSRYTAGWRSVGASLSDVAAMGAEATAAVAVYGAPQFDADEIAAFIEGCLDVCEAVDAEYVGGDLDGHEEFTVATTAFGQTDAPVYRSGASPGDAVCVTGTLGRSAAALRRFEAGDTERGNELFRFEPRVAAGRGVAPHATAMMDSSDGLARSLHQLAAASGCGFAIEADAIPVAPAVEEVADGPSDRRELAVHFGEDFELVATMPETAVDAAAEASPVALTRVGTVTESGVTMDGEALPDRGYTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVZ1</accession>
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        <submittedName>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REPRESSOR YCNK"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI49235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="202" mass="22047" checksum="4F207F921AB692AF" modified="2017-05-10" version="1">MQQRLSDSRRRRTVLAAIGAGAAGLAGCLDGGSSNDPEDDASPEVSDQSFPDHPVTEPRDPPEGRRCDGPCGMVAAEYPDWNAQVAHADGQGAFFDTPGCLVAYYQHHTFYDGPPAEIEGVWVRDFETTELLDAAEAYFVLDTDDGRHAEPMGSNPKPFADRDDAVSYVESYQDLTTDDIVGLDEFGPEEAHVYRDYPLDEA</sequence>
</entry>
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    <accession>A0A1U7EW00</accession>
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        <submittedName>
            <fullName evidence="1">Small CPxCG-related zinc finger protein</fullName>
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    </protein>
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        <name evidence="1" type="ordered locus">NP_2332A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
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            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW00"/>
    <dbReference type="STRING" id="348780.NP_2332A"/>
    <dbReference type="EnsemblBacteria" id="CAI49257">
        <property type="protein sequence ID" value="CAI49257"/>
        <property type="gene ID" value="NP_2332A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701409"/>
    <dbReference type="KEGG" id="nph:NP_2332A"/>
    <dbReference type="HOGENOM" id="CLU_2565846_0_0_2"/>
    <dbReference type="OrthoDB" id="384576at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="81" mass="8926" checksum="A0459FB8715C40AE" modified="2017-05-10" version="1">MSEPRGDGGTERTPQGSVHEHDCVVCGTLVRYVDFCPHIVLCLDCGYPDPAPCVEADEDGYAYIVTNDMTKDDTDNTEDNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW05</accession>
    <name>A0A1U7EW05_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2334A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW05"/>
    <dbReference type="STRING" id="348780.NP_2334A"/>
    <dbReference type="EnsemblBacteria" id="CAI49258">
        <property type="protein sequence ID" value="CAI49258"/>
        <property type="gene ID" value="NP_2334A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701410"/>
    <dbReference type="KEGG" id="nph:NP_2334A"/>
    <dbReference type="HOGENOM" id="CLU_2911623_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="61" mass="6647" checksum="8F6E337669627AFD" modified="2017-05-10" version="1">MSTTDDRELICLTSRSAVTDAGSVRVNIPAELLDAVDTDPDDIEDVIIRGYDDGNVEIRLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW22</accession>
    <name>A0A1U7EW22_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2350A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322892.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW22"/>
    <dbReference type="STRING" id="348780.NP_2350A"/>
    <dbReference type="EnsemblBacteria" id="CAI49266">
        <property type="protein sequence ID" value="CAI49266"/>
        <property type="gene ID" value="NP_2350A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701418"/>
    <dbReference type="KEGG" id="nph:NP_2350A"/>
    <dbReference type="HOGENOM" id="CLU_2581522_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="80" mass="9351" checksum="5D3C5D42A415CAC1" modified="2017-05-10" version="1">MTDDTELTNDDRIDELTAEIDDLESRLDYLADLTVDRIDPPDHVDEQVLRGSLKVDRRKVRTKLDTKRRQLEAAREATNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW27</accession>
    <name>A0A1U7EW27_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Homolog to phage PhiH1 repressor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2364A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322899.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW27"/>
    <dbReference type="STRING" id="348780.NP_2364A"/>
    <dbReference type="EnsemblBacteria" id="CAI49273">
        <property type="protein sequence ID" value="CAI49273"/>
        <property type="gene ID" value="NP_2364A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701423"/>
    <dbReference type="KEGG" id="nph:NP_2364A"/>
    <dbReference type="eggNOG" id="arCOG03924">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161782_1_0_2"/>
    <dbReference type="OrthoDB" id="285635at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="107" mass="11948" checksum="335EF1233670E4CB" modified="2017-05-10" version="1">MRRSAEWMVLCDERILEYLSENESGTPSEMANSGRVRWTRSYISQRAKKLVDHGLLKHLGNGVYIITDEGEAYLDEEYDAERGAYLDRNVADADSDGAESAGEANGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW30</accession>
    <name>A0A1U7EW30_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2368A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322901.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW30"/>
    <dbReference type="STRING" id="348780.NP_2368A"/>
    <dbReference type="EnsemblBacteria" id="CAI49275">
        <property type="protein sequence ID" value="CAI49275"/>
        <property type="gene ID" value="NP_2368A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701425"/>
    <dbReference type="KEGG" id="nph:NP_2368A"/>
    <dbReference type="eggNOG" id="ENOG502N62I">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2140275_0_0_2"/>
    <dbReference type="OrthoDB" id="221753at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="112" mass="12219" checksum="623BE419415814A3" modified="2017-05-10" version="1">MTGDPLSRLFVEDSDDRPDEVVVDELTTMDAGEFVARLESLAEAAEAVSTMAGDVERLRQTGLSDEDARDLIYGRNNSLAKRDIEAMFETVDAVVSGRADRPAKRLLSELSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW45</accession>
    <name>A0A1U7EW45_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ArNOG06768 family NADH-binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">nolA1</name>
        <name evidence="2" type="ordered locus">NP_2406A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322920.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW45"/>
    <dbReference type="STRING" id="348780.NP_2406A"/>
    <dbReference type="EnsemblBacteria" id="CAI49294">
        <property type="protein sequence ID" value="CAI49294"/>
        <property type="gene ID" value="NP_2406A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702979"/>
    <dbReference type="KEGG" id="nph:NP_2406A"/>
    <dbReference type="eggNOG" id="arCOG03015">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_6_5_2"/>
    <dbReference type="OrthoDB" id="213145at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05271">
        <property type="entry name" value="NDUFA9_like_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000702">
        <property type="entry name" value="NADH dehydrogenase (Ubiquinone)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051207">
        <property type="entry name" value="ComplexI_NDUFA9_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126:SF11">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 39 KDA SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="1">
        <location>
            <begin position="7"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="305" mass="31974" checksum="941D56E26D161C7F" modified="2017-05-10" version="1">MELLVVGGTGFIGTHLCAELHDRGHEVTAMSRSPDDGGVPEGVEATAGDVTTYDSIEPAFEGVDAVVNLVALSPLFRPSGGDEMHYRIHRDGTENVVAAAEKHGVDRLVQLSALGADPDGETAYIRAKGQGEDIVRSSSLEWVILRPSVVFGDGGEFIPYTKQLAPAYLTPLPGGGKTRFQPIWIGDLAPMIADAATEDEHAGEIYELGGPEALTLASIAKLAHAADGRPVNVLPIPMAVAGAGLKALGSVPESVLDSLPGVPRMGGDQYRSLQFDNTTDENDVAAFGVDSGTLRTVSDYLSIDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW61</accession>
    <name>A0A1U7EW61_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Plant-type L-asparaginase</fullName>
            <ecNumber evidence="1">3.5.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">L-asparagine amidohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ansA</name>
        <name evidence="8" type="ordered locus">NP_2432A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-asparagine + H2O = L-aspartate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:21016"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="EC" id="3.5.1.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.5.1.1" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322933.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW61"/>
    <dbReference type="STRING" id="348780.NP_2432A"/>
    <dbReference type="EnsemblBacteria" id="CAI49307">
        <property type="protein sequence ID" value="CAI49307"/>
        <property type="gene ID" value="NP_2432A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701442"/>
    <dbReference type="KEGG" id="nph:NP_2432A"/>
    <dbReference type="eggNOG" id="arCOG04779">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021603_1_2_2"/>
    <dbReference type="OrthoDB" id="18230at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd04703">
        <property type="entry name" value="Asparaginase_2_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.30">
        <property type="entry name" value="(Glycosyl)asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000246">
        <property type="entry name" value="Peptidase_T2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10188">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10188:SF6">
        <property type="entry name" value="N(4)-(BETA-N-ACETYLGLUCOSAMINYL)-L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01112">
        <property type="entry name" value="Asparaginase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="200"/>
            <end position="203"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="222"/>
            <end position="225"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Cleavage; by autolysis" evidence="7">
        <location>
            <begin position="172"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00030414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00044776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="294" mass="29865" checksum="771E23C6E7AF272E" modified="2017-05-10" version="1">MRTIVHGGAGTAPAEPNERLAVLEGAADAGADAQTPTEAVCAAIRRLESAPRFNAGVGSAVQSDGYIRTDAGLMTDDRSVGAAAGMPGVEHAIDVARTVKDETPHVLVAGVHAVDLAETVGVETTVDLWTDRTRQRWDDLDTKPPDELGDQLAWVTDRFGGDPAGRDGTDHDTVGAVATDGDRIAAGTSTGGRWLALAGRVGDVPQVGSGFYCTPAGGVSATGAGEDIARQTLARSAADRLDAGDDAQRAVEAAIDDFDEAVEGTAGLIALTPDGDAGAAYSSEAMQTAVSGTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW65</accession>
    <name>A0A1U7EW65_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Esterase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2408A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322921.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW65"/>
    <dbReference type="STRING" id="348780.NP_2408A"/>
    <dbReference type="EnsemblBacteria" id="CAI49295">
        <property type="protein sequence ID" value="CAI49295"/>
        <property type="gene ID" value="NP_2408A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701437"/>
    <dbReference type="KEGG" id="nph:NP_2408A"/>
    <dbReference type="eggNOG" id="arCOG00777">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142277_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016289">
        <property type="term" value="F:acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03443">
        <property type="entry name" value="PaaI_thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052723">
        <property type="entry name" value="Acyl-CoA_thioesterase_PaaI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003736">
        <property type="entry name" value="PAAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00369">
        <property type="entry name" value="unchar_dom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42856">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE PAAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42856:SF1">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE PAAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioesterase" evidence="2">
        <location>
            <begin position="49"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="145" mass="16009" checksum="573D4EE150C4C0C0" modified="2017-05-10" version="1">MDHYRKLERMYHETPCNSDLDLTLSITGPGEAELRLAVDESMHHAADGVHGSYYFKALDDATFFAANSLIEDVFVLTTDFHIQLTRPVSTGQLRAEAEVVNDHPRQLIADGVLYDGDGNQLARGTGTFGKSDVELDAEMGYELSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWE0</accession>
    <name>A0A1U7EWE0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">glutaryl-CoA dehydrogenase (ETF)</fullName>
            <ecNumber evidence="9">1.3.8.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">acd1</name>
        <name evidence="15" type="ordered locus">NP_2620A</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>glutaryl-CoA + oxidized [electron-transfer flavoprotein] + 2 H(+) = (2E)-butenoyl-CoA + reduced [electron-transfer flavoprotein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:13389"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57332"/>
            <dbReference type="ChEBI" id="CHEBI:57378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="EC" id="1.3.8.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid metabolism; lysine degradation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid metabolism; tryptophan metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.6" evidence="9"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323026.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWE0"/>
    <dbReference type="STRING" id="348780.NP_2620A"/>
    <dbReference type="EnsemblBacteria" id="CAI49401">
        <property type="protein sequence ID" value="CAI49401"/>
        <property type="gene ID" value="NP_2620A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702473"/>
    <dbReference type="KEGG" id="nph:NP_2620A"/>
    <dbReference type="eggNOG" id="arCOG01707">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_8_0_2"/>
    <dbReference type="OrthoDB" id="275197at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000062">
        <property type="term" value="F:fatty-acyl-CoA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004361">
        <property type="term" value="F:glutaryl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046949">
        <property type="term" value="P:fatty-acyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052033">
        <property type="entry name" value="Glutaryl-CoA_DH_mitochondrial"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42807">
        <property type="entry name" value="GLUTARYL-COA DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42807:SF1">
        <property type="entry name" value="GLUTARYL-COA DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0274">FAD</keyword>
    <keyword evidence="3 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6 11 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="14">
        <location>
            <begin position="13"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="13">
        <location>
            <begin position="127"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="12">
        <location>
            <begin position="234"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAI49401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="387" mass="42579" checksum="87903EA3A3CBBCF1" modified="2017-05-10" version="1">MLDFVQLEGDLDQEERLIRDTAREFVEEHVKPDIGEHFQEGTFPTELIPKMGELGFYAPNLEGYGSPNVSETAYGLLMQELEAGDSGLRSMASVQGALVMYPIHAYGSEAQKEEWLPKLGQGEAVGCFGLTEPEHGSNPSAMETHAEADGDGYVLNGAKTWITNSPISDVAVVWARDRSADDTPVRGFLVETDRDGVTTNKITDKLSLRASITGEIGLNDVYVPEENVLPGVSGMKGPLSCLTQARYGIAWGAVGAARDAFEEARQYATDRDQFGGPIGRFQLQQDKLAEMATQITLAQLLAYRLAELKERGDMRPQHVSMSKRNNVRMARNQARIAREMLGGNGITTDYSPMRHMANLETVYTYEGTHDIHTLILGEDLTGLQAYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWM3</accession>
    <name>A0A1U7EWM3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type transport system permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">abc23p2</name>
        <name evidence="7" type="ordered locus">NP_2798A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49490.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939556.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWM3"/>
    <dbReference type="STRING" id="348780.NP_2798A"/>
    <dbReference type="EnsemblBacteria" id="CAI49490">
        <property type="protein sequence ID" value="CAI49490"/>
        <property type="gene ID" value="NP_2798A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702438"/>
    <dbReference type="KEGG" id="nph:NP_2798A"/>
    <dbReference type="eggNOG" id="arCOG01470">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066801_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12698">
        <property type="entry name" value="ABC2_membrane_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="164"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="251"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="279"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="317"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="ABC-2 type transporter transmembrane" evidence="6">
        <location>
            <begin position="21"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49490.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="347" mass="36741" checksum="9B2A0D2189847895" modified="2017-05-10" version="1">MSKTTIAGRELRSLSREKTIVLALLIQLVIAGFSSFLVVGLTSLYEPGAAADDVIVGATGEETDALVEAAGEVDGLVVRPYDDRSEARAAFEDNRVQATAHVERAEGRIAVRIEAPQASVQKTFIVVQLRAALETLERNERADRSEYLSVLPLSVPPSVDASPYFGFSYTVLVPLLVFLPVFISGAVVVDSLTEEIERGTLELLRVTPASLLDIVDGKAGVMAVLTPLQVVLWLALLAINDIAIANVAPILAIATSFAALAVVFAAALAVAIPVRQRAQLTYSFGMLAAFAAVALLPEHPATSIALFAINSPTTTTYAHLAGYCLVAVAAVVAFRWWIGTLDAESLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWR6</accession>
    <name>A0A1U7EWR6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">dihydrofolate reductase</fullName>
            <ecNumber evidence="2">1.5.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">folA</name>
        <name evidence="8" type="ordered locus">NP_2922A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the dihydrofolate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.3" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323176.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWR6"/>
    <dbReference type="STRING" id="348780.NP_2922A"/>
    <dbReference type="EnsemblBacteria" id="CAI49552">
        <property type="protein sequence ID" value="CAI49552"/>
        <property type="gene ID" value="NP_2922A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702722"/>
    <dbReference type="KEGG" id="nph:NP_2922A"/>
    <dbReference type="eggNOG" id="arCOG01490">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043966_5_1_2"/>
    <dbReference type="OrthoDB" id="198183at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004146">
        <property type="term" value="F:dihydrofolate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046452">
        <property type="term" value="P:dihydrofolate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046655">
        <property type="term" value="P:folic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00209">
        <property type="entry name" value="DHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="entry name" value="Dihydrofolate Reductase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012259">
        <property type="entry name" value="DHFR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017925">
        <property type="entry name" value="DHFR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001796">
        <property type="entry name" value="DHFR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48069">
        <property type="entry name" value="DIHYDROFOLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48069:SF3">
        <property type="entry name" value="DIHYDROFOLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00186">
        <property type="entry name" value="DHFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000194">
        <property type="entry name" value="DHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00070">
        <property type="entry name" value="DHFR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="Dihydrofolate reductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00075">
        <property type="entry name" value="DHFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51330">
        <property type="entry name" value="DHFR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHFR" evidence="7">
        <location>
            <begin position="2"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="165" mass="17982" checksum="F35AA7E03A242CBF" modified="2017-05-10" version="1">MKLVLIAAVAENGVIGTDGEMPWHYPEDLKRFKETTMGHPVIMGRTTYESITGQLGGPLPGRTNIVLSSRESLPIPEEVVHARDIEAALSAAEDALDADKQTVYVAGGATVYEQCLDRADELRITEVPESPDGDTYFPAIGDEWTATEREAGDEVAFVRYERAGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWV1</accession>
    <name>A0A1U7EWV1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7998 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2974A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323202.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWV1"/>
    <dbReference type="EnsemblBacteria" id="CAI49578">
        <property type="protein sequence ID" value="CAI49578"/>
        <property type="gene ID" value="NP_2974A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701612"/>
    <dbReference type="KEGG" id="nph:NP_2974A"/>
    <dbReference type="eggNOG" id="arCOG06271">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113978_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058311">
        <property type="entry name" value="DUF7998"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25979">
        <property type="entry name" value="DUF7998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7998" evidence="2">
        <location>
            <begin position="15"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="204" mass="22253" checksum="039D9E66FD2C6C54" modified="2017-05-10" version="1">MAGFPSPFGGSDESTFDSEDEFVPEHLPEPGPFLDAHDVLTGDRHVAFHDVTETVFEERTVYDMTFGYNLARLNLDTRHPGAGYRYAVEADDETVLRAEFTPTTDFCPQTDTLTVGSFRAWNGLDDHHGYDLVRVRVVSSHHQIEAVNQKLESLEAEFLETGEVPAGDGTSEPTDNTAAAGPAVGGPNGPVPERDDDDGPETPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWX4</accession>
    <name>A0A1U7EWX4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2988A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323209.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWX4"/>
    <dbReference type="STRING" id="348780.NP_2988A"/>
    <dbReference type="EnsemblBacteria" id="CAI49585">
        <property type="protein sequence ID" value="CAI49585"/>
        <property type="gene ID" value="NP_2988A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701617"/>
    <dbReference type="KEGG" id="nph:NP_2988A"/>
    <dbReference type="eggNOG" id="arCOG11485">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074511_0_0_2"/>
    <dbReference type="OrthoDB" id="241667at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="296"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="327" mass="31805" checksum="1162CAB970DAF592" modified="2017-05-10" version="1">MQRLLRSVGVAPLAAAAISVGIAGVGSAVTTPPTPLSAVGGVVLASLVIAVGYVSAHEVGTELFPQHRIRLGVADGADFVAVVVGGVLTRWLAVEAALGVVVASALVGLVAWLVVPERAAAAYCGSFVGMAAPAVLPGYATVAAAGGVAGTVFILAAGVYSGFGGKLGATAFVGCTAVVLGTGAVPTSEPVPHVGTAVGLTAVAVVAAVLTYVLSVRLAYGPVAASAAVGLAAGGLAPVVFAEGTPVAAVAFCASFTGMVAESRLPGEPLVGVAGLATGIFFVATTPYFGGFGGKLGAIAFVGCLATYGLAVLSPWHCISPRRRRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX07</accession>
    <name>A0A1U7EX07_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3124A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323275.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX07"/>
    <dbReference type="STRING" id="348780.NP_3124A"/>
    <dbReference type="EnsemblBacteria" id="CAI49653">
        <property type="protein sequence ID" value="CAI49653"/>
        <property type="gene ID" value="NP_3124A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701659"/>
    <dbReference type="KEGG" id="nph:NP_3124A"/>
    <dbReference type="HOGENOM" id="CLU_1850697_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="138" mass="14116" checksum="BDACE96596AC4426" modified="2017-05-10" version="1">MTDDLSDQRCQQCGGVGEAVESGGVTTIECSDCGNVLGLADPTQERSAAEATSDTAGASDTGADVDTVGPPGDETGQPDDTVGTVTAVDGEFDQLLRVLQSQDGRITASTLRIELPAETLSVTAVDGRIRIETDGGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX82</accession>
    <name>A0A1U7EX82_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TrmB family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3370A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323396.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX82"/>
    <dbReference type="STRING" id="348780.NP_3370A"/>
    <dbReference type="EnsemblBacteria" id="CAI49776">
        <property type="protein sequence ID" value="CAI49776"/>
        <property type="gene ID" value="NP_3370A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701753"/>
    <dbReference type="KEGG" id="nph:NP_3370A"/>
    <dbReference type="eggNOG" id="arCOG02241">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082928_1_0_2"/>
    <dbReference type="OrthoDB" id="59565at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="162" mass="17725" checksum="977C8F1CE48B672E" modified="2017-05-10" version="1">MATDKSSDDEGESTAGDSDGVRDRLEQEAERAAEQFDDGIVDLLSWVLDTETRARIYVFLRQNPGSTSEEIAEGTGLYPSTVREALAGLHEEGRLERQKRDADGAGNNPYEYTAMAPSELVGDAVGEIQDELNTVFNLDSHLGVCEDDDTEPVTITVERDDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX98</accession>
    <name>A0A1U7EX98_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphopantetheine adenylyltransferase</fullName>
            <ecNumber evidence="7">2.7.7.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Dephospho-CoA pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Pantetheine-phosphate adenylyltransferase</fullName>
            <shortName evidence="7">PPAT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">coaD</name>
        <name evidence="9" type="ordered locus">NP_3368A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Reversibly transfers an adenylyl group from ATP to 4'-phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(R)-4'-phosphopantetheine + ATP + H(+) = 3'-dephospho-CoA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19801"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="EC" id="2.7.7.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the eukaryotic CoaD family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.3" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323395.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX98"/>
    <dbReference type="STRING" id="348780.NP_3368A"/>
    <dbReference type="EnsemblBacteria" id="CAI49775">
        <property type="protein sequence ID" value="CAI49775"/>
        <property type="gene ID" value="NP_3368A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701752"/>
    <dbReference type="KEGG" id="nph:NP_3368A"/>
    <dbReference type="eggNOG" id="arCOG01223">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035272_5_0_2"/>
    <dbReference type="OrthoDB" id="53228at2157"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004595">
        <property type="term" value="F:pantetheine-phosphate adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00647">
        <property type="entry name" value="PPAT_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023540">
        <property type="entry name" value="PPAT_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001985">
        <property type="entry name" value="PRK00777.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 7 9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="8">
        <location>
            <begin position="4"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI49775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="163" mass="18029" checksum="FA69BD41EE746C46" modified="2017-05-10" version="1">MKVVLGGTFDPVHDGHRALFERAFELGDVTVGLTSDELAPKTRSVDRHVRSYDERKDDLEAELETLADDYGREFEVRELTEPTGIATEPGFDALIVSPETKDGGELVNEERIEKGLEPLDIEVVDHRYAEDGDIISSTRIVAGEIDEHGNLTPESDGRPPAKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXB8</accession>
    <name>A0A1U7EXB8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3478A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323450.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXB8"/>
    <dbReference type="STRING" id="348780.NP_3478A"/>
    <dbReference type="EnsemblBacteria" id="CAI49830">
        <property type="protein sequence ID" value="CAI49830"/>
        <property type="gene ID" value="NP_3478A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701784"/>
    <dbReference type="KEGG" id="nph:NP_3478A"/>
    <dbReference type="eggNOG" id="arCOG06363">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1003318_0_0_2"/>
    <dbReference type="OrthoDB" id="330337at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="277" mass="29887" checksum="7548CEA67ED5B089" modified="2017-05-10" version="1">MTRPRVLWATPPDRRPQSAAAVELETAATESNAQERIDTLSETDRRVDAAVAAPSFTDGDGPGVLHAVREAWPDAACLLYGDPWAVPDGRSPPVCEIHPATQSPMAVVDAARTAVRQRYHRPYPVFEDERRRLQLVDAADFEVARADFRGHLEDLVEKTAADAATLAVVDDYTTTVVASTAGTHGVVVPRGDTPAAYTIDKPEPITIPDLDADARLPDDTPAYGGDYRSYVGHSLWAESVPLAAVELFADDNTLADPPTDTLSWHVADLEAAIESVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXC2</accession>
    <name>A0A1U7EXC2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable PrkA-type serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">prkA1</name>
        <name evidence="2" type="ordered locus">NP_3444A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXC2"/>
    <dbReference type="STRING" id="348780.NP_3444A"/>
    <dbReference type="EnsemblBacteria" id="CAI49813">
        <property type="protein sequence ID" value="CAI49813"/>
        <property type="gene ID" value="NP_3444A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3444A"/>
    <dbReference type="eggNOG" id="arCOG02891">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028588_2_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013153">
        <property type="entry name" value="Prk_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010650">
        <property type="entry name" value="PrkA_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30267">
        <property type="entry name" value="PROTEIN KINASE PRKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30267:SF2">
        <property type="entry name" value="PROTEIN PRKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08298">
        <property type="entry name" value="AAA_PrkA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06798">
        <property type="entry name" value="PrkA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00763">
        <property type="entry name" value="AAA_PrkA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PrkA AAA" evidence="1">
        <location>
            <begin position="23"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="686" mass="79299" checksum="CF2F997B2F10A426" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXR6</accession>
    <name>A0A1U7EXR6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ABC-type transport system periplasmic substrate-binding protein (Probable substrate cobalamin)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">btuF</name>
        <name evidence="4" type="synonym">abc16s:fhuD</name>
        <name evidence="4" type="ordered locus">NP_3814A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323615.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXR6"/>
    <dbReference type="STRING" id="348780.NP_3814A"/>
    <dbReference type="EnsemblBacteria" id="CAI49998">
        <property type="protein sequence ID" value="CAI49998"/>
        <property type="gene ID" value="NP_3814A"/>
    </dbReference>
    <dbReference type="GeneID" id="59558372"/>
    <dbReference type="KEGG" id="nph:NP_3814A"/>
    <dbReference type="eggNOG" id="arCOG04233">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038034_2_1_2"/>
    <dbReference type="OrthoDB" id="214567at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071281">
        <property type="term" value="P:cellular response to iron ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1980">
        <property type="entry name" value="Nitrogenase molybdenum iron protein domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050902">
        <property type="entry name" value="ABC_Transporter_SBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026469">
        <property type="entry name" value="Peripla_PGF_1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04281">
        <property type="entry name" value="peripla_PGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30535:SF34">
        <property type="entry name" value="MOLYBDATE-BINDING PROTEIN MOLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30535">
        <property type="entry name" value="VITAMIN B12-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53807">
        <property type="entry name" value="Helical backbone' metal receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="320"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="3">
        <location>
            <begin position="59"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="346" mass="36129" checksum="5FF92AD75F904F77" modified="2017-05-10" version="1">MRTLSAVVFAIALVAVAAGAPVAAAPAADTAPADPTCSFPVTETDATGTEVTVEERPERIVVLQPSAAQTVWELGAEDRVVGAPVVPYTSDLDGIEEAENVLQVDDFTVNQEAVVELDADLVLAPNIVPDDTVTSLREADQQVFKFGFGTSLSFVADKTALTGQLVDACDAAAEVNDDYWDRIDAVEERAAAVEDPHVMYFTDNHTAGSGTFIDDLITTAGGVNVAAEAGIDGYDEINEEALVEWNPDVIVASDAEPLPETAAYESTVAVEHGQVVTVDSNDVSQPAPRLVYALETIADGLADAETADRPTDATVDDQPGVGIAPAAAGLVLAVVAGLLVRTLRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXR9</accession>
    <name>A0A1U7EXR9_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fumarate hydratase class II</fullName>
            <shortName evidence="5">Fumarase C</shortName>
            <ecNumber evidence="5">4.2.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Aerobic fumarase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Iron-independent fumarase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 8" type="primary">fumC</name>
        <name evidence="8" type="ordered locus">NP_3778A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(S)-malate = fumarate + H2O</text>
            <dbReference type="Rhea" id="RHEA:12460"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="EC" id="4.2.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="5">There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.2" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49980.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939632.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXR9"/>
    <dbReference type="STRING" id="348780.NP_3778A"/>
    <dbReference type="EnsemblBacteria" id="CAI49980">
        <property type="protein sequence ID" value="CAI49980"/>
        <property type="gene ID" value="NP_3778A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702732"/>
    <dbReference type="KEGG" id="nph:NP_3778A"/>
    <dbReference type="eggNOG" id="arCOG01749">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021594_4_1_2"/>
    <dbReference type="OrthoDB" id="26319at2157"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER01007"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004333">
        <property type="term" value="F:fumarate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006106">
        <property type="term" value="P:fumarate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01362">
        <property type="entry name" value="Fumarase_classII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.40.30:FF:000002">
        <property type="entry name" value="Fumarate hydratase class II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.275.10:FF:000001">
        <property type="entry name" value="Fumarate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.200.10:FF:000001">
        <property type="entry name" value="Fumarate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.40.30">
        <property type="entry name" value="Fumarase/aspartase (C-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00743">
        <property type="entry name" value="FumaraseC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005677">
        <property type="entry name" value="Fum_hydII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018951">
        <property type="entry name" value="Fumarase_C_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008909">
        <property type="entry name" value="PRK12273.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11444">
        <property type="entry name" value="ASPARTATEAMMONIA/ARGININOSUCCINATE/ADENYLOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11444:SF22">
        <property type="entry name" value="FUMARATE HYDRATASE CLASS II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10415">
        <property type="entry name" value="FumaraseC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00145">
        <property type="entry name" value="ARGSUCLYASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Fumarate lyase N-terminal" evidence="6">
        <location>
            <begin position="16"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="Fumarase C C-terminal" evidence="7">
        <location>
            <begin position="411"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="5">
        <location>
            <position position="191"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="321"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="102"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="in site B" evidence="5">
        <location>
            <begin position="132"/>
            <end position="135"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="142"/>
            <end position="144"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="327"/>
            <end position="329"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="5">
        <location>
            <position position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49980.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="471" mass="50597" checksum="F133EE76F68B8340" modified="2017-05-10" version="1">MADEDTDYRIERDSLGEMEVPADAYWGAQTQRALGNFPVSGITFQRRFIRALGIVKKSAAKANLDLELLEADKAEAIIEAADEVIAGEHDDQFPVDVFQTGSGTSSNMNANEVIANRATEIYGGEIGTREIHPNDHVNYGQSSNDVIPTAMHVASLEAARKDVLPGLETLAEELAAKEDEFDAVVKTGRTHLQDATPVRLGQEFGGYRTQIEKGIDRVEGATERLEELALGGTATGTGLNTHPEFPERAAEYISEETGLSFREADNHFEAQAAHDAMSEAHGALRTVAGSMNKIANDLRLLASGPRNGLGEVEQPENQPGSSIMPGKINPVVAESVNQVHKQVIGNDAAVSAGAARGEIDLNLYKPVLAYNFLQSASIIASSAELFGERFVGKLEANADHCRTKVEQSMALATALNPAIGYDKASKVAKQALKEGKTVREVAVEEGYLTEAEADEVLDPKAMTERVILGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXT4</accession>
    <name>A0A1U7EXT4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3862A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323639.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXT4"/>
    <dbReference type="EnsemblBacteria" id="CAI50022">
        <property type="protein sequence ID" value="CAI50022"/>
        <property type="gene ID" value="NP_3862A"/>
    </dbReference>
    <dbReference type="GeneID" id="41345104"/>
    <dbReference type="KEGG" id="nph:NP_3862A"/>
    <dbReference type="HOGENOM" id="CLU_1860759_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="137" mass="15152" checksum="BC826290DB697198" modified="2017-05-10" version="1">MAVANLVDSYGGAPVDGLISDFHSIEMIGQSSVDSELPKLRIWWDDPDAVDADFVETETCFVEEVYGGGGSQNQSLKIHVADSYEDADLQSSVEFEQVRYARRTVSHPRGGDVSVVVVRFDHHPTQMGEEIIEVHVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXT6</accession>
    <name>A0A1U7EXT6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3838A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323627.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXT6"/>
    <dbReference type="STRING" id="348780.NP_3838A"/>
    <dbReference type="EnsemblBacteria" id="CAI50010">
        <property type="protein sequence ID" value="CAI50010"/>
        <property type="gene ID" value="NP_3838A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701880"/>
    <dbReference type="KEGG" id="nph:NP_3838A"/>
    <dbReference type="eggNOG" id="arCOG06752">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_764213_0_0_2"/>
    <dbReference type="OrthoDB" id="2572at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="2"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="368" mass="42352" checksum="FCE85ED2D4A66192" modified="2017-05-10" version="1">MSDCRYCEQTFNEETALRKHLYEAHERDELSRIDRKRVEQYIDEHSVDESSNDADTDGSQSIQREQRTVNTSDGTYPADAWELSDVEALSTDEIVAKLEDHGIETNETAFRNRAEDLDSATTLSEQWEAEYEVDATGYDQDFIWMAAQELWERWIPEVPNKERIYDFVQEGRDLREKGNRAGACNQWLTAWEYILAVIPDESTTIEEADRTLPTFLSLEAFIRSLDSDLATVAEGNPAYHEHRLEFCREVCDQFPDADDEFLLDFRHFVADSLAELGRTADSRAELEALIEEYPEDPYAYKKLADSYWLDEPDELSMEELERTAELYRAALDVEGPLEGASMVADCLEDVENRLTDARASDERGGEKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXW1</accession>
    <name>A0A1U7EXW1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Homolog to phage PhiH1 repressor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3896A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323656.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXW1"/>
    <dbReference type="EnsemblBacteria" id="CAI50039">
        <property type="protein sequence ID" value="CAI50039"/>
        <property type="gene ID" value="NP_3896A"/>
    </dbReference>
    <dbReference type="GeneID" id="32155018"/>
    <dbReference type="KEGG" id="nph:NP_3896A"/>
    <dbReference type="eggNOG" id="arCOG03924">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161782_1_0_2"/>
    <dbReference type="OrthoDB" id="285635at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="118" mass="14042" checksum="A88151B6656D434C" modified="2017-05-10" version="1">MRKQGDWMANWDDRFLEFAYDHGGATPKRIADNEHVHVSRQYVAKRLRELSDHGLLNKIGRGSYTITKKGKYYLAGAYDAEKDEFLEYELRNYEWLILESEDKIEDFRSWFNDRSSAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXX3</accession>
    <name>A0A1U7EXX3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3944A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323680.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXX3"/>
    <dbReference type="STRING" id="348780.NP_3944A"/>
    <dbReference type="EnsemblBacteria" id="CAI50063">
        <property type="protein sequence ID" value="CAI50063"/>
        <property type="gene ID" value="NP_3944A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701919"/>
    <dbReference type="KEGG" id="nph:NP_3944A"/>
    <dbReference type="eggNOG" id="arCOG00498">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048478_0_2_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07725">
        <property type="entry name" value="TTHA1429-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050662">
        <property type="entry name" value="Sec-metab_biosynth-thioest"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23131">
        <property type="entry name" value="ENDORIBONUCLEASE LACTB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23131:SF4">
        <property type="entry name" value="METALLO-BETA-LACTAMASE SUPERFAMILY POTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="23"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="344" mass="36731" checksum="CF6C1FE50D4A7D07" modified="2017-05-10" version="1">MTDPALPFELGVLSLGNTAFEGNNSSYVLGTEPEATTTLIDTGVAEPTTEQQLRDGLDRQGLSVADIERVFLTHHHSDHAGLAGEIQAESGCTVYVHEADAPLVEQDSAAMAAQSERLRVTIDRWGMPDAKQTELLRFLDATDGIGGRPPTVTTFDGGERFDLGSVTLEAVHMPGHAAGLAGYAFEGRDGTELFSGDALLPYYTPNVGGADLRVDGALAAYLDTLAGIIEREYSRAWPGHRGPIIDPTGRAADIVAHHRERTERVVSVLRSGPATPWEVSAELFGSLSGIHVLHGPGEAFAHLDHLSEARVVTESDGTFELVVSDPELDPLFPDVAGTRTEEMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXY9</accession>
    <name>A0A1U7EXY9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC-type transport system periplasmic substrate-binding protein (Probable substrate sulfate/thiosulfate/molybdate)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">abc13s</name>
        <name evidence="3" type="ordered locus">NP_3928A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial solute-binding protein 1 family. WtpA subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323672.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXY9"/>
    <dbReference type="STRING" id="348780.NP_3928A"/>
    <dbReference type="EnsemblBacteria" id="CAI50055">
        <property type="protein sequence ID" value="CAI50055"/>
        <property type="gene ID" value="NP_3928A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702410"/>
    <dbReference type="KEGG" id="nph:NP_3928A"/>
    <dbReference type="eggNOG" id="arCOG00219">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055936_2_0_2"/>
    <dbReference type="OrthoDB" id="7820at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030973">
        <property type="term" value="F:molybdate ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015689">
        <property type="term" value="P:molybdate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13540">
        <property type="entry name" value="PBP2_ModA_WtpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050682">
        <property type="entry name" value="ModA/WtpA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30632">
        <property type="entry name" value="MOLYBDATE-BINDING PERIPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30632:SF16">
        <property type="entry name" value="MOLYBDATE_TUNGSTATE-BINDING PROTEIN WTPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13531">
        <property type="entry name" value="SBP_bac_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="291"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="311" mass="32833" checksum="FB4D0DAC879262AD" modified="2017-05-10" version="1">MVSNGTEASCSRRSVLAAVGTGIAGGVAGCLDSSASVSVLAAGSLAQTVEHHVGPAFESETDTAVHGEYYGSNALLRMVEDRMKTPDVVVSADATLLRDRLYDEYVSGDVEFATNVLGICYAPSTELGERLDAGEPWYEPVRDADDGAVAITEPDLDPLGYRALLAFELAERKHGLDGFRDTVGEAVYREPEEPQLLAGVAAGNRAAAISYRNMAVDHDLPFRSFPPAYNFADPAYADDYATVSFVTDDGYEVVGRPIVYNAAIPDDADNPDAAESLVSFLATSPSLLETAGLSVPEPYPQTAGAEPGVDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY04</accession>
    <name>A0A1U7EY04_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Beta-lactamase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">NP_4032A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323723.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY04"/>
    <dbReference type="STRING" id="348780.NP_4032A"/>
    <dbReference type="EnsemblBacteria" id="CAI50107">
        <property type="protein sequence ID" value="CAI50107"/>
        <property type="gene ID" value="NP_4032A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701947"/>
    <dbReference type="KEGG" id="nph:NP_4032A"/>
    <dbReference type="eggNOG" id="arCOG00504">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030571_5_1_2"/>
    <dbReference type="OrthoDB" id="197151at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06262">
        <property type="entry name" value="metallo-hydrolase-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051453">
        <property type="entry name" value="MBL_Glyoxalase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46233">
        <property type="entry name" value="HYDROXYACYLGLUTATHIONE HYDROLASE GLOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46233:SF3">
        <property type="entry name" value="HYDROXYACYLGLUTATHIONE HYDROLASE GLOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="5">
        <location>
            <begin position="14"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI50107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="193" mass="20824" checksum="5D0B071AF6BB55C3" modified="2017-05-10" version="1">MTVRNIARGIRSFTSNAFLVDGERTVLVDTGNDFDAVSNVDAATGDLDAVVLTHTHPDHVGNVPAVVEAFDVEVWGYDGDHDLVDHEVGDGDTVDIGDHPYEVLHTPGHKEDHVCLYAPTAATLFAGDLVFANGGFGRTDLPEGDRDQLIESIEYLLDAVDESLSTMYVGHGPTVDADPRHHIELAHGAARMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY52</accession>
    <name>A0A1U7EY52_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rps10b</name>
        <name evidence="4" type="synonym">rps10</name>
        <name evidence="6" type="ordered locus">NP_4090A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in the binding of tRNA to the ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323752.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY52"/>
    <dbReference type="STRING" id="348780.NP_4090A"/>
    <dbReference type="EnsemblBacteria" id="CAI50136">
        <property type="protein sequence ID" value="CAI50136"/>
        <property type="gene ID" value="NP_4090A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701968"/>
    <dbReference type="KEGG" id="nph:NP_4090A"/>
    <dbReference type="eggNOG" id="arCOG01758">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153624_0_0_2"/>
    <dbReference type="OrthoDB" id="333791at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.600">
        <property type="entry name" value="Ribosomal protein S10 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00508">
        <property type="entry name" value="Ribosomal_uS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001848">
        <property type="entry name" value="Ribosomal_uS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027486">
        <property type="entry name" value="Ribosomal_uS10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036838">
        <property type="entry name" value="Ribosomal_uS10_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00338">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01403">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54999">
        <property type="entry name" value="Ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS10" evidence="5">
        <location>
            <begin position="6"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI50136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="109" mass="12221" checksum="8EDE6FCFABEFD78C" modified="2017-05-10" version="1">MPFVTTLTLQSGDRDVLERVVGDIKERAERKGVELRGPHAETPADVSVPQSKRLEANGSSYSHWRYTVYERRMEIVGHDDFARSVAGRDFPDGVHIDVDVERVSAPGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYA3</accession>
    <name>A0A1U7EYA3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thioesterase domein protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4246A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323830.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYA3"/>
    <dbReference type="STRING" id="348780.NP_4246A"/>
    <dbReference type="EnsemblBacteria" id="CAI50214">
        <property type="protein sequence ID" value="CAI50214"/>
        <property type="gene ID" value="NP_4246A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702011"/>
    <dbReference type="KEGG" id="nph:NP_4246A"/>
    <dbReference type="eggNOG" id="arCOG04331">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1754746_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025540">
        <property type="entry name" value="FlK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054485">
        <property type="entry name" value="FlK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36934">
        <property type="entry name" value="BLR0278 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36934:SF1">
        <property type="entry name" value="THIOESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22636">
        <property type="entry name" value="FlK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fluoroacetyl-CoA-specific thioesterase-like" evidence="1">
        <location>
            <begin position="49"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="152" mass="15401" checksum="701778CF75FDC0C7" modified="2017-05-10" version="1">MTTHNAPSALVGATGTTTFTVAAAHTTVVFGHQQSPPGVPAATDSSPDEAIRLLGTAHLLARVEFTGRESIAGEIPPGTGVVGKTATVAHRGAAPVGTEVVVETEVVGVDGADIRIDGDANADGRTVGRAEVTLQLVDRERFRDGLEAAPDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYC0</accession>
    <name>A0A1U7EYC0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">IclR family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">NP_4258A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323836.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYC0"/>
    <dbReference type="STRING" id="348780.NP_4258A"/>
    <dbReference type="EnsemblBacteria" id="CAI50220">
        <property type="protein sequence ID" value="CAI50220"/>
        <property type="gene ID" value="NP_4258A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702012"/>
    <dbReference type="KEGG" id="nph:NP_4258A"/>
    <dbReference type="eggNOG" id="arCOG02798">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062618_6_1_2"/>
    <dbReference type="OrthoDB" id="14763at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050707">
        <property type="entry name" value="HTH_MetabolicPath_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014757">
        <property type="entry name" value="Tscrpt_reg_IclR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005471">
        <property type="entry name" value="Tscrpt_reg_IclR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30136">
        <property type="entry name" value="HELIX-TURN-HELIX TRANSCRIPTIONAL REGULATOR, ICLR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30136:SF35">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR RV1719"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09339">
        <property type="entry name" value="HTH_IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01614">
        <property type="entry name" value="IclR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00346">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51077">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51078">
        <property type="entry name" value="ICLR_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH iclR-type" evidence="4">
        <location>
            <begin position="10"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="IclR-ED" evidence="5">
        <location>
            <begin position="70"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI50220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="259" mass="27853" checksum="EDF893C3B6706675" modified="2017-05-10" version="1">MIPDTSSREVKSVRTAFRIIDILRDYDGVDIDELADRLGLAKSTVHNYLSTLRGMGYVVERDGSYRLGLRFLTEGMAAQSSLRLRPAVAEVIGSVAETVGQPTWWVVEEHGRGIFVESAIPPGTRRIYGRVGKRSYLHTHAPGKAILAALSDEYIEDIVDFHGLPGHTERTHTDLDSLREDIETARKRGFAVADGEAALGVRSVGVAFEGPSGYTHGLGVFGYSHDFGIPPESDITAALSAAADELAAAGQNDTTEGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYC8</accession>
    <name>A0A1U7EYC8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Acyl-CoA dehydrogenase</fullName>
            <ecNumber evidence="10">1.3.8.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">acd4</name>
        <name evidence="10" type="ordered locus">NP_4254A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.-" evidence="10"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323834.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYC8"/>
    <dbReference type="STRING" id="348780.NP_4254A"/>
    <dbReference type="EnsemblBacteria" id="CAI50218">
        <property type="protein sequence ID" value="CAI50218"/>
        <property type="gene ID" value="NP_4254A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702479"/>
    <dbReference type="KEGG" id="nph:NP_4254A"/>
    <dbReference type="eggNOG" id="arCOG04350">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_0_2_2"/>
    <dbReference type="OrthoDB" id="275197at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00567">
        <property type="entry name" value="ACAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000002">
        <property type="entry name" value="Acyl-CoA dehydrogenase fadE12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF12">
        <property type="entry name" value="ISOVALERYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016578">
        <property type="entry name" value="HsaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0274">FAD</keyword>
    <keyword evidence="3 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 6 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="9">
        <location>
            <begin position="6"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="8">
        <location>
            <begin position="125"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="7">
        <location>
            <begin position="234"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI50218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="388" mass="42659" checksum="C4EC53FD3A5DCC10" modified="2017-05-10" version="1">MTFETSPEHSMIASTATEIAEQFGPEYWRQKEENEAFAEDFWDELGEAGFHGLAVPEEYGGAGMGMQELGLAMETLCAEGCGMAGTWYLVLTAGMAAVGIKQHGDEQQKETYLTDIATGDRNFSIGITEPEAGTNTLNVATTAEKDGDEYVLNGQKAWITFSDRADNMILVTRTTPRSEVDRGTDGISLFIVDMDSPGIEVSPIEKHAMNYSKSCEVFLEDVRVPEADLLGEEGDGWRALVSMLNPERIGFAAAGVGIGKLAAHTAIDYANDREVFGDPIGTHQAVSFPITKPYARLEAAKLMRQKAEWLYDQGEDCGYETNIAKATAVEAGIETVKQSMQAFGGWGYATEYDVERWWREINLTRLAPVSQQMAYNHLSQEIGFPKSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYD0</accession>
    <name>A0A1U7EYD0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF3054 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4290A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323852.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYD0"/>
    <dbReference type="STRING" id="348780.NP_4290A"/>
    <dbReference type="EnsemblBacteria" id="CAI50236">
        <property type="protein sequence ID" value="CAI50236"/>
        <property type="gene ID" value="NP_4290A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702022"/>
    <dbReference type="KEGG" id="nph:NP_4290A"/>
    <dbReference type="eggNOG" id="arCOG03922">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143267_0_0_2"/>
    <dbReference type="OrthoDB" id="177006at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021414">
        <property type="entry name" value="DUF3054"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11255">
        <property type="entry name" value="DUF3054"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="139" mass="14990" checksum="E87293D3A62BCEAC" modified="2017-05-10" version="1">MLSTRYADRWTVSVRRTALVAVGDIVAIAAFLLYGLLIHAVNPLFFPQHTVLAAAPFIFAWVITAPLGGLYRPETVTSVRSALTKTTAVWVVASLAGGAIRGTEYFPGEAPAVFLVVNIVFGLAFLLPWRLAVAYTLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYM5</accession>
    <name>A0A1U7EYM5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">arginine decarboxylase</fullName>
            <ecNumber evidence="3">4.1.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">pdaD</name>
        <name evidence="8" type="ordered locus">NP_4484A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-arginine + H(+) = agmatine + CO2</text>
            <dbReference type="Rhea" id="RHEA:17641"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:58145"/>
            <dbReference type="EC" id="4.1.1.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PdaD family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.19" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323948.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYM5"/>
    <dbReference type="STRING" id="348780.NP_4484A"/>
    <dbReference type="EnsemblBacteria" id="CAI50333">
        <property type="protein sequence ID" value="CAI50333"/>
        <property type="gene ID" value="NP_4484A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702076"/>
    <dbReference type="KEGG" id="nph:NP_4484A"/>
    <dbReference type="eggNOG" id="arCOG04490">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134253_0_0_2"/>
    <dbReference type="OrthoDB" id="30748at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008792">
        <property type="term" value="F:arginine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006527">
        <property type="term" value="P:L-arginine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.20.10">
        <property type="entry name" value="Pyruvoyl-Dependent Histidine Decarboxylase, subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016104">
        <property type="entry name" value="Pyr-dep_his/arg-deCO2ase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016105">
        <property type="entry name" value="Pyr-dep_his/arg-deCO2ase_sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002724">
        <property type="entry name" value="Pyruvoyl-dep_arg_deCO2ase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40438">
        <property type="entry name" value="PYRUVOYL-DEPENDENT ARGININE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40438:SF1">
        <property type="entry name" value="PYRUVOYL-DEPENDENT ARGININE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01862">
        <property type="entry name" value="PvlArgDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005216">
        <property type="entry name" value="Pyruvoyl-dep_arg_deCO2ase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01170">
        <property type="entry name" value="Pyruvoyl-dependent_arginine_de"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00055">
        <property type="entry name" value="Pyruvoyl-Dependent_Histidine/A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56271">
        <property type="entry name" value="Pyruvoyl-dependent histidine and arginine decarboxylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="5 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI50333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="159" mass="16551" checksum="008D9A46D891F593" modified="2017-05-10" version="1">MGRIRIVWGTATAPTAMASYDAALAAANVHNYNLVAVSSVVPADADIEVAGTAPDLGPDGERLTVVEGRTTVPPEKDRSAAAALGWARDSDGPGIFYEVSGSDPDAVEKRLDAGLDAGLELREWDCDVTGRRVVHADADPDGYTTALVLAVYGDSDPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYP6</accession>
    <name>A0A1U7EYP6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Amine oxidase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4520A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323966.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYP6"/>
    <dbReference type="STRING" id="348780.NP_4520A"/>
    <dbReference type="EnsemblBacteria" id="CAI50351">
        <property type="protein sequence ID" value="CAI50351"/>
        <property type="gene ID" value="NP_4520A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702812"/>
    <dbReference type="KEGG" id="nph:NP_4520A"/>
    <dbReference type="eggNOG" id="arCOG01522">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051347_0_0_2"/>
    <dbReference type="OrthoDB" id="11867at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050464">
        <property type="entry name" value="Zeta_carotene_desat/Oxidored"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005560">
        <property type="entry name" value="PRK07233.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42923">
        <property type="entry name" value="PROTOPORPHYRINOGEN OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42923:SF3">
        <property type="entry name" value="PROTOPORPHYRINOGEN OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="1">
        <location>
            <begin position="9"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="445" mass="49153" checksum="D4350583EAC24693" modified="2017-05-10" version="1">MIGIVGGGLAGLAAAYRLQQAGHEVRIFEASETVGGLAATYETAGDPIERYYHHLSKSEETIVDLAADLGVADRIEWRVGENAYYVDGVSHPLDTPWEIAAYPYLSVYDKFRLTMLTLEVDVRGGRPRRDTYESLADFEDVPIREFLIEHTTRGVYEYFFEPLLDAKFGDRKEDVSAAWLLGRVKFRGERDLLRGEILGYLDGGFGVFVDALVDAVGREHIETGSRVRELDFAETPDTTGRGPVESLTVADADGDATTHAVDGVVVATMPDVLESLTGYTCDIDFQGSVCAVVSMDESLLDTYWLNIADDAPFGALIEHTNFVPAERYGGEHLLYVASYVQQLADDLWQRSDDEIEALWLDGIADMFPQFDRDRVNWVAISRNPKTAPVYERGYLDMVVPYDLTDDGAPGVYYAGMASRAQYPERSLNGAIEAGFAAADGVLDAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYX4</accession>
    <name>A0A1U7EYX4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4710A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYX4"/>
    <dbReference type="STRING" id="348780.NP_4710A"/>
    <dbReference type="EnsemblBacteria" id="CAI50446">
        <property type="protein sequence ID" value="CAI50446"/>
        <property type="gene ID" value="NP_4710A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_4710A"/>
    <dbReference type="eggNOG" id="arCOG03920">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_596686_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045466">
        <property type="entry name" value="DUF6498"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20108">
        <property type="entry name" value="DUF6498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="218"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="296"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="334"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="458" mass="50035" checksum="E983A857DABB034B" modified="2017-05-10" version="1">MGAFPIEIAQFVGAWNDNSETKHPFSPGLYRHRERCPHDPRTPGVLGRVVQHLSVLLMQPSTRDRPLAALLGANLLPLAGVLALGWDPVLVLLLYWLEIGVVLVFNVPKVLLAQATRDGRLPDDIALWDGGPAFRLNLLWLAVYALVIAGVLHILVAVVTPLEATLAQLLRSDAPPQVAGTVLLAAVGTVFGHAMALRRFITDRESQRVTPTFQVFRPAVYLWLLAGIVMLADGGLVSEDQFAAVVVGIILAKSGFDILTERAVTITPPTLPDRFRRTPPPGQPALCVSPASRPLLAAYVPLSLVGVGLAATLGFFTYGLFIVELAGLGSRGELLWGVLYVLSTVCFTAAAVWAIHRRYTNIEYRIYSSGIAVYNTHHDTLVRFVPATDIRSCEPVSGPITWLFSAASVRVRYRQRRRFGTIAIGPFDIEMPELRDEDEDGELTLQYVGNADSIRAML</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYY8</accession>
    <name>A0A1U7EYY8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Bisanhydrobacterioruberin hydratase</fullName>
            <ecNumber evidence="2">4.2.1.161</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cruF</name>
        <name evidence="2" type="ordered locus">NP_4768A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.2.1.161" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324087.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYY8"/>
    <dbReference type="STRING" id="348780.NP_4768A"/>
    <dbReference type="EnsemblBacteria" id="CAI50475">
        <property type="protein sequence ID" value="CAI50475"/>
        <property type="gene ID" value="NP_4768A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702170"/>
    <dbReference type="KEGG" id="nph:NP_4768A"/>
    <dbReference type="eggNOG" id="arCOG02835">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070738_0_0_2"/>
    <dbReference type="OrthoDB" id="107798at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053540">
        <property type="entry name" value="BABR_hydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017823">
        <property type="entry name" value="CruF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007354">
        <property type="entry name" value="CruF-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03460">
        <property type="entry name" value="crt_membr_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041333">
        <property type="entry name" value="CruF_Halo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39419">
        <property type="entry name" value="SLL0814 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39419:SF1">
        <property type="entry name" value="SLL0814 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04240">
        <property type="entry name" value="Caroten_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="235"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="275" mass="30092" checksum="D751206CF8B99095" modified="2017-05-10" version="1">MRSFELPARPHVERRLDELVDENRFTIAVVFPAVGAITLLGSAEGVLPEPLSFNPYFLLFGVAVMRLPLIAGIAPLLTKRAAAGLLVLCGYTYAIEFTGVLTGYPYGHFEYGVDLGPMLAGHVPAALPLFFLPLVVNAYLLCLLLLGSLARRAAVRIPIVVAAVVGMDLVLDPAAVAVGFWAYDAGGLYYQVPWTNYAGWVLSATVSVVVLDRAFDWNGLLARVESCRFMLDDLVSFVILWGVVNAYFGNWLPALLAVGYGYGLWRTDRFDFPER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYZ6</accession>
    <name>A0A1U7EYZ6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cystathionine synthase/lyase (Cystathionine gamma-synthase, cystathionine gamma-lyase, cystathionine beta-lyase)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">metB</name>
        <name evidence="5" type="synonym">metC</name>
        <name evidence="5" type="ordered locus">NP_4746A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
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            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
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    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PRK07811.1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CYSTATHIONINE GAMMA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRANS-SULFURATION ENZYME FAMILY MEMBER"/>
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        <location>
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            <begin position="1"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009077"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI50464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="399" mass="42993" checksum="26C7FF567D8A1C56" modified="2017-05-10" version="1">MEPTDPSDETDEEPADGRIETQAIHAGQSPDAETGAIMPPIYATSTYEQDAPGEDRGYEYSRTGNPTRTALEDNLAALEGGDYGRAFASGMASINTVLNLLSAGDHVVAGDDVYGGTHRLFREVFEQYDLSFSFVDTTDLDAVEAAIRPETELLWVETPTNPLLRINDIEALSAIAHDHDALCAVDNTFATPYLQRPLEHGADIVSHSLTKYLGGHSDVVGGALVTDDADLDEQLGFYQNSVGATPDPFACFLVLRGTKTLPVRMDRHCANAETIAEKLDAHPAVDRVHYPGLDDHPGHELAAEQMDGFGGMVSVELDATLEETREVVSSTEVFTLAESLGGVESLLEQPATMTHASIPPEERRAAGLTDGLIRLSVGTEHVDDLWADLEAAIEDAVEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZB5</accession>
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        <submittedName>
            <fullName evidence="4">DUF4212 family protein</fullName>
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        <name evidence="4" type="ordered locus">NP_5134A</name>
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        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
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            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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    <dbReference type="EnsemblBacteria" id="CAI50658">
        <property type="protein sequence ID" value="CAI50658"/>
        <property type="gene ID" value="NP_5134A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702266"/>
    <dbReference type="KEGG" id="nph:NP_5134A"/>
    <dbReference type="eggNOG" id="arCOG06362">
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    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="entry name" value="Na_symporter_ssu"/>
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        <property type="entry name" value="Na_symport_sm"/>
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    </dbReference>
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        <location>
            <begin position="66"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium symporter small subunit" evidence="3">
        <location>
            <begin position="57"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="162"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
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        <location>
            <begin position="16"/>
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        </location>
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        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="162" mass="17887" checksum="562539CEAD258E03" modified="2017-05-10" version="1">MADKHMHENDSHETEAADTGDTPDDGELVTDGGMSDVEREKEIDYLDVEINLLKPATPFMRDHLRVIWLGFGIWILTTFGPVTATRLAPELMTSQMPLIGFPLHYFLIAIGGPSAALILSVWYARKRDQIDEKYGIEQTVSEPEPAETVAEEAAATDGGVDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZD8</accession>
    <name>A0A1U7EZD8_NATPD</name>
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        <submittedName>
            <fullName evidence="2">Cupin 2 barrel domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5146A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
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            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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            <authorList>
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
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                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="NP_5146A"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR053146">
        <property type="entry name" value="QDO-like"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR36440">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_8G07350)-RELATED"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR36440:SF1">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="44"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="123" mass="13215" checksum="6BD0E57A172F580E" modified="2017-05-10" version="1">MSQPTPLIRRANDVEYDTVDAADGLRKGVLVGPDDGAPTFALRRFVLEAGATVPEHTNEVEHEQYVLEGEYVVGIGDEEHTVSAGDSLLIPAGAVHWYRNEGDAPGAFLCAVPNGDDEIQLVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZG1</accession>
    <name>A0A1U7EZG1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF302 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5178A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324290.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZG1"/>
    <dbReference type="STRING" id="348780.NP_5178A"/>
    <dbReference type="EnsemblBacteria" id="CAI50680">
        <property type="protein sequence ID" value="CAI50680"/>
        <property type="gene ID" value="NP_5178A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702283"/>
    <dbReference type="KEGG" id="nph:NP_5178A"/>
    <dbReference type="eggNOG" id="arCOG02761">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126998_1_1_2"/>
    <dbReference type="OrthoDB" id="193855at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd14797">
        <property type="entry name" value="DUF302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.70">
        <property type="entry name" value="TT1751-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005180">
        <property type="entry name" value="DUF302"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035923">
        <property type="entry name" value="TT1751-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016796">
        <property type="entry name" value="UCP021774"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38342">
        <property type="entry name" value="SLR5037 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38342:SF1">
        <property type="entry name" value="SLR5037 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03625">
        <property type="entry name" value="DUF302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF021774">
        <property type="entry name" value="UCP021774"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103247">
        <property type="entry name" value="TT1751-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF302" evidence="1">
        <location>
            <begin position="51"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="148" mass="16434" checksum="1A2922B3E4250CEB" modified="2017-05-10" version="1">MSLAFDPSKIDAEDIGEEQTTLAMDHEAAVEHVRTVAEETGFGIPVEFSPSELLNEKVDADRDPYYVLGACNPEMADRALDETLKQGALFPCNFVVWQEEPGRQRVYHVSIMRIARLVGMAPDNEAWGDIVDDTGELVAAFFEALDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZI7</accession>
    <name>A0A1U7EZI7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Adenosylcobinamide amidohydrolase</fullName>
            <ecNumber evidence="2">3.5.1.90</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cbiZ</name>
        <name evidence="2" type="ordered locus">NP_5300A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.1.90" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324350.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZI7"/>
    <dbReference type="STRING" id="348780.NP_5300A"/>
    <dbReference type="EnsemblBacteria" id="CAI50741">
        <property type="protein sequence ID" value="CAI50741"/>
        <property type="gene ID" value="NP_5300A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702326"/>
    <dbReference type="KEGG" id="nph:NP_5300A"/>
    <dbReference type="eggNOG" id="arCOG01870">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087823_0_0_2"/>
    <dbReference type="OrthoDB" id="157452at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043756">
        <property type="term" value="F:adenosylcobinamide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002808">
        <property type="entry name" value="AdoCbi_amidolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052209">
        <property type="entry name" value="CbiZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35336">
        <property type="entry name" value="ADENOSYLCOBINAMIDE AMIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35336:SF5">
        <property type="entry name" value="ADENOSYLCOBINAMIDE AMIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01955">
        <property type="entry name" value="CbiZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="229" mass="23299" checksum="54C026687E69800B" modified="2017-05-10" version="1">MFDFERTSDRLTVREPGTRWLSNGFDGGYTVADAAHNLTVPDGFGRTDLDAYAAERLGEPPAGPTLLTGVNQQHARGGRLGPVVAVVTAGLSNPATLPMAASADDGRTDGADREWRPGTVNIVVGTRRPLSDGGLAGLLATAVEAKTATLSAVAGCTGTTSDAVAVGCPSGDTPEPFAGSATEVGAAVRACVREALLASLASRYEDGPPAPADAEHGVETDDRAAVFQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZJ9</accession>
    <name>A0A1U7EZJ9_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Multidrug-efflux transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">NP_5292A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50737.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_148215466.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZJ9"/>
    <dbReference type="STRING" id="348780.NP_5292A"/>
    <dbReference type="EnsemblBacteria" id="CAI50737">
        <property type="protein sequence ID" value="CAI50737"/>
        <property type="gene ID" value="NP_5292A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702323"/>
    <dbReference type="KEGG" id="nph:NP_5292A"/>
    <dbReference type="eggNOG" id="arCOG01731">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012893_5_3_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13137">
        <property type="entry name" value="MATE_NorM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050222">
        <property type="entry name" value="MATE_MdtK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048279">
        <property type="entry name" value="MdtK-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43298:SF2">
        <property type="entry name" value="FMN_FAD EXPORTER YEEO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43298">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN NORM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006603">
        <property type="entry name" value="DinF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="209"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="343"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="373"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="410"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="436"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI50737.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="478" mass="49045" checksum="14A4E1B2660F4371" modified="2017-05-10" version="1">MPNLFRLGVLAVGLALARAGLIDETRARRTTELAWPRIVTGIARMSKNAVDVAMVGVAVGSSAIAGVGIAGPFWGLAFAIGGGIAGGTIALVSQRYGADAYDELGQAVRTSAFLVVIASLPLVAVFWTYGPQLVGLLSSDPEVVRLGGDYLRLVGFGIPLAGLNLVGSRVLVGSDDAYTAMVVRAGGAVANVVINAVLIFGLGMGVEGAALGTVLSNAAVTAAFALGLTFGRLPGAGRFPVTVDPVGGYVDRATARDLIAIGAPVMGRNLVWTVAEIPMFAILDIFGSDVVSAFVIARRVWGLLNTPGWGFGLASSSLVGQELGTGDESTAEAYGRDIIRFSVAVYIVSAAVVFAFAGPIVEAFVDDPTGPEVPIAVDLVRVACVAIVLQGVSGGAAGPLDAAGDTRWPFASQALGMFGVSIPLVLVGALTPLGIYGLYLAFLAETAVPAALNYYRFRSGAWKAVSREYRPEAGRADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZK7</accession>
    <name>A0A1U7EZK7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5340A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324370.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZK7"/>
    <dbReference type="STRING" id="348780.NP_5340A"/>
    <dbReference type="EnsemblBacteria" id="CAI50761">
        <property type="protein sequence ID" value="CAI50761"/>
        <property type="gene ID" value="NP_5340A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702340"/>
    <dbReference type="KEGG" id="nph:NP_5340A"/>
    <dbReference type="HOGENOM" id="CLU_2581522_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="7"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="84" mass="9881" checksum="A725DAFA5D500C97" modified="2017-05-10" version="1">MTDELTQLANDERAAELERRIDDLESRLEWLEATDPDDLDAPDDADESVLRGKIKMRRRLAREQLKRCREMYNELTNDEGADDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZN3</accession>
    <name>A0A1U7EZN3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Small CPxCG-related zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2369E</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CCI69579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_162010748.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZN3"/>
    <dbReference type="STRING" id="348780.NP_2369E"/>
    <dbReference type="EnsemblBacteria" id="CCI69579">
        <property type="protein sequence ID" value="CCI69579"/>
        <property type="gene ID" value="NP_2369E"/>
    </dbReference>
    <dbReference type="GeneID" id="41345087"/>
    <dbReference type="KEGG" id="nph:NP_2369E"/>
    <dbReference type="HOGENOM" id="CLU_190388_0_0_2"/>
    <dbReference type="OrthoDB" id="189700at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055985">
        <property type="entry name" value="DUF7563"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24444">
        <property type="entry name" value="DUF7563"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCI69579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="84" mass="8979" checksum="6A46EC21E1FBB217" modified="2017-05-10" version="1">MSNGECLSCGSHVSQSFVRVFGDGGNLYACPSCTTREAIKRGAAADPDHDRRVTVPPSDARRQAGDTPRATWDGRRTTSPEGVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILT2</accession>
    <name>Q3ILT2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3320A</name>
        <name evidence="3" type="ordered locus">NP_7044A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>PL23</name>
    </geneLocation>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
            <strain evidence="3">Gabara</strain>
            <plasmid evidence="3">PL23</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CR936259">
        <property type="protein sequence ID" value="CAI50938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323372.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILT2"/>
    <dbReference type="STRING" id="348780.NP_3320A"/>
    <dbReference type="EnsemblBacteria" id="CAI49751">
        <property type="protein sequence ID" value="CAI49751"/>
        <property type="gene ID" value="NP_3320A"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI50938">
        <property type="protein sequence ID" value="CAI50938"/>
        <property type="gene ID" value="NP_7044A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703482"/>
    <dbReference type="KEGG" id="nph:NP_3320A"/>
    <dbReference type="KEGG" id="nph:NP_7044A"/>
    <dbReference type="eggNOG" id="arCOG09042">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_584784_0_0_2"/>
    <dbReference type="OrthoDB" id="271889at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="260"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="326"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="458"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="467" mass="51038" checksum="17FB99BE1E2231E9" modified="2005-11-08" version="1">MRTFQNTSVTEVEFGRLDYARQARDELPNEALDVDDDGREKRLIVDKDAIDRRTMSRVEGLAADSRDSARGDMHGQAELTQSERNQLDFSRTTIPEARTAKASLQSEGVDDWVAHFDPTLTTSEMADKARDSAIGGGQRMDAEDSVRAQDRRHAEAFREVQGQAERRARDGCEQGFEDACDQLVEEFGFERAEAEQLLRQRQAEAVAQADLQDTSFLRAATNGRYLQEPPEPAPNAPGWRKASTGRFIGYDLVDPRAKRHPETGHIAETDPIELSRREARARYGDPDGGSTSPGSREEAKREALFEFEPMDRGEDIMAQAAAADVTLSPDEADPEYPAGATGRTAMGGFNPHYGGEVAPNTNTPLDETPPDIARGDDPFDMSENGGLLSDDRDRQGTLDAGLDAGTTRDRSGEIAGSAGFEDRRDELQPDNDAGEQTGLVEVESDIGDGQTDLFGGSATETTGGEYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM37</accession>
    <name>Q3IM37_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphate-specific transport system accessory protein PhoU</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">phoU3</name>
        <name evidence="9" type="synonym">abc15r1</name>
        <name evidence="9" type="ordered locus">NP_6096A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9 10">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Plays a role in the regulation of phosphate uptake.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the PhoU family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324430.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM37"/>
    <dbReference type="EnsemblBacteria" id="CAI50828">
        <property type="protein sequence ID" value="CAI50828"/>
        <property type="gene ID" value="NP_6096A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694672"/>
    <dbReference type="KEGG" id="nph:NP_6096A"/>
    <dbReference type="HOGENOM" id="CLU_078518_3_0_2"/>
    <dbReference type="OrthoDB" id="7738at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:intracellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045936">
        <property type="term" value="P:negative regulation of phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.220:FF:000004">
        <property type="entry name" value="Phosphate-specific transport system accessory protein PhoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="entry name" value="Phosphate transport system protein phou homolog 2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028366">
        <property type="entry name" value="PhoU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026022">
        <property type="entry name" value="PhoU_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02135">
        <property type="entry name" value="phoU_full"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930">
        <property type="entry name" value="PHOSPHATE-SPECIFIC TRANSPORT SYSTEM ACCESSORY PROTEIN PHOU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930:SF3">
        <property type="entry name" value="PHOSPHATE-SPECIFIC TRANSPORT SYSTEM ACCESSORY PROTEIN PHOU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01895">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003107">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109755">
        <property type="entry name" value="PhoU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PhoU" evidence="8">
        <location>
            <begin position="18"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="PhoU" evidence="8">
        <location>
            <begin position="121"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR003107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI50828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="221" mass="24911" checksum="81E759D9F86CC843" modified="2005-11-08" version="1">MARKDYQRKLDELRSDVVSMSDLVLDRYNRALTTMETKDEPMAQSVIEGDEDVNQLYLDLESDCIELFALQQPVAGDLRFIASTFKIITDLERIGDFATNLAGYAIEAEQNRYSEIDISYIGTEAGAMVGDAMAAYADADADRAREIAARDDRIDRLCEEAGEAVMQDLIETQYNDNIEAVVEDASRLLLTVRDLERVSDHAVNICARTVYMAEHDDELIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM56</accession>
    <name>Q3IM56_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF8154 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_6050A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324411.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM56"/>
    <dbReference type="EnsemblBacteria" id="CAI50805">
        <property type="protein sequence ID" value="CAI50805"/>
        <property type="gene ID" value="NP_6050A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694693"/>
    <dbReference type="KEGG" id="nph:NP_6050A"/>
    <dbReference type="HOGENOM" id="CLU_133601_0_0_2"/>
    <dbReference type="OrthoDB" id="237859at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058467">
        <property type="entry name" value="DUF8154"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26481">
        <property type="entry name" value="DUF8154"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8154" evidence="1">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="165" mass="18164" checksum="690BDC36808AAD0F" modified="2005-11-08" version="1">MSSTRIEHLIDEVQAAFDRRPTDIEAGLDVEDAALLQLRKACRLLAGAESLRDASYYTLVIEASFVAIERTVEFRLLERGTMQPDDLPGTHPGVYREAAAAGVFGESVAADLADLWRDHRAKTYYQDGLASAARADAMYELATELHAYVTGRSRQGHECLCGETT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM70</accession>
    <name>Q3IM70_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">HTH domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_6018A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50789.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939897.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM70"/>
    <dbReference type="EnsemblBacteria" id="CAI50789">
        <property type="protein sequence ID" value="CAI50789"/>
        <property type="gene ID" value="NP_6018A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694583"/>
    <dbReference type="KEGG" id="nph:NP_6018A"/>
    <dbReference type="HOGENOM" id="CLU_142662_1_0_2"/>
    <dbReference type="OrthoDB" id="195563at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="2">
        <location>
            <begin position="27"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50789.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="119" mass="14029" checksum="38702C8596D2080F" modified="2012-10-03" version="2">MSSGTIDIDEFENADADDFAERNDTERIVRFLDAHDDRAWKAATIAERLELETDAVSAILSRLKDRGLVRHKRPYWAITDDTERLQSAYRLHRHHERADDQYGEERLENLQTDEMDRVQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-07-10" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMW8</accession>
    <name>RS5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein uS5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">30S ribosomal protein S5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps5</name>
        <name type="ordered locus">NP_4894A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">With S4 and S12 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Contacts protein S4.</text>
    </comment>
    <comment type="domain">
        <text>The N-terminal domain interacts with the head of the 30S subunit; the C-terminal domain interacts with the body and contacts protein S4. The interaction surface between S4 and S5 is involved in control of translational fidelity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324150.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMW8"/>
    <dbReference type="SMR" id="Q3IMW8"/>
    <dbReference type="STRING" id="348780.NP_4894A"/>
    <dbReference type="EnsemblBacteria" id="CAI50538">
        <property type="protein sequence ID" value="CAI50538"/>
        <property type="gene ID" value="NP_4894A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703184"/>
    <dbReference type="KEGG" id="nph:NP_4894A"/>
    <dbReference type="eggNOG" id="arCOG04087">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065898_0_1_2"/>
    <dbReference type="OrthoDB" id="38155at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000002">
        <property type="entry name" value="40S ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000004">
        <property type="entry name" value="40S ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01307_A">
        <property type="entry name" value="Ribosomal_uS5_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000851">
        <property type="entry name" value="Ribosomal_uS5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047866">
        <property type="entry name" value="Ribosomal_uS5_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005324">
        <property type="entry name" value="Ribosomal_uS5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005711">
        <property type="entry name" value="Ribosomal_uS5_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013810">
        <property type="entry name" value="Ribosomal_uS5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018192">
        <property type="entry name" value="Ribosomal_uS5_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003125">
        <property type="entry name" value="PRK04044.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01020">
        <property type="entry name" value="uS5_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718:SF4">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718">
        <property type="entry name" value="RIBOSOMAL S SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00333">
        <property type="entry name" value="Ribosomal_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03719">
        <property type="entry name" value="Ribosomal_S5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00585">
        <property type="entry name" value="RIBOSOMAL_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50881">
        <property type="entry name" value="S5_DSRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000293215" description="Small ribosomal subunit protein uS5">
        <location>
            <begin position="1"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="S5 DRBM" evidence="1">
        <location>
            <begin position="46"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="197"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="211" mass="23036" checksum="263B2B8140BA5D2F" modified="2005-11-08" version="1">MSNGWEPRTRLGRKVADGEITSMQDALQSGLPLKEPELVDQLLPGLEDEVLDINMVQRMTDSGRRVKFRCVVVIGNRDGYVGYAQGRDDQVGGAIQKAIDVAKLNIIDVPLGSGSWEDRPGGRNSLMRRTTGKAGSVEVELKPAPRGLGLAAAPTVRHVLELAGVEDAWTKCHGNTRTTLNLAKATYNALRNASESRTPEHTRQVRQEAQE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMY4</accession>
    <name>RS19_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein uS19</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">30S ribosomal protein S19</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps19</name>
        <name type="ordered locus">NP_4862A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324134.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMY4"/>
    <dbReference type="SMR" id="Q3IMY4"/>
    <dbReference type="STRING" id="348780.NP_4862A"/>
    <dbReference type="EnsemblBacteria" id="CAI50522">
        <property type="protein sequence ID" value="CAI50522"/>
        <property type="gene ID" value="NP_4862A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703173"/>
    <dbReference type="KEGG" id="nph:NP_4862A"/>
    <dbReference type="eggNOG" id="arCOG04099">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097347_1_0_2"/>
    <dbReference type="OrthoDB" id="30559at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.860.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.860.10">
        <property type="entry name" value="30s Ribosomal Protein S19, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00531">
        <property type="entry name" value="Ribosomal_uS19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002222">
        <property type="entry name" value="Ribosomal_uS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020934">
        <property type="entry name" value="Ribosomal_uS19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005713">
        <property type="entry name" value="Ribosomal_uS19_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023575">
        <property type="entry name" value="Ribosomal_uS19_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003121">
        <property type="entry name" value="PRK04038.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01025">
        <property type="entry name" value="uS19_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880">
        <property type="entry name" value="RIBOSOMAL PROTEIN S19P FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00203">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002144">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00975">
        <property type="entry name" value="RIBOSOMALS19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54570">
        <property type="entry name" value="Ribosomal protein S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00323">
        <property type="entry name" value="RIBOSOMAL_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000265473" description="Small ribosomal subunit protein uS19">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="140" mass="15974" checksum="9F0C99F5BAD07DCC" modified="2005-11-08" version="1">MSSEYQIGHEGEFTYRGHTLDELQEMELDEVAELLPARQRRTIKRGLSVEQEKLLDEAREAGEEETANNPIRTHLRDMPILPRFVGLTFAVYNGQSFERVEVEPEMIGHYLGEFQLTRTSVEHGQAGIGATRSSKFVPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IN25</accession>
    <name>Q3IN25_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">3'-5' ssDNA/RNA exonuclease TatD</fullName>
            <ecNumber evidence="1">3.1.11.-</ecNumber>
            <ecNumber evidence="1">3.1.13.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tatD</name>
        <name evidence="1" type="ordered locus">NP_4780A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.11.-" evidence="1"/>
    <dbReference type="EC" id="3.1.13.-" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324093.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IN25"/>
    <dbReference type="STRING" id="348780.NP_4780A"/>
    <dbReference type="EnsemblBacteria" id="CAI50481">
        <property type="protein sequence ID" value="CAI50481"/>
        <property type="gene ID" value="NP_4780A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702172"/>
    <dbReference type="KEGG" id="nph:NP_4780A"/>
    <dbReference type="eggNOG" id="arCOG00893">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_985571_0_0_2"/>
    <dbReference type="OrthoDB" id="52767at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011589">
        <property type="entry name" value="UCP004961"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42206:SF1">
        <property type="entry name" value="METAL-DEPENDENT HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42206">
        <property type="entry name" value="METAL-DEPENDENT HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004961">
        <property type="entry name" value="UCP004961_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="280" mass="30246" checksum="A47C3D3A0659B336" modified="2005-11-08" version="1">MYDGPILDNHLHLDPVNGRGADAAAEFADAGGTHLNVLNKPSWNLVGEVDDEDGFRETFDITIDVTAAADELLPGRAWPVLGVHPALISQLVDRGYGPEEAAELMCAGIDVAAEYVASGRALAIKSGRPHYDVADAVWEASNQVMRHAFARAAEVGCAVQLHTEGGEDFETVAEWAEAEGLARKQVVKHYSAGYVDGPIPSVIAHKDELERACGGEWPFFMETDFIDDPDRPGAVLGPKTVPKRTRWLAEAGHETALRRAHVETPAQVYGIDTEATLDEN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q3INI5</accession>
    <name>RL11_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL11</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">50S ribosomal protein L11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl11</name>
        <name type="ordered locus">NP_4454A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the ribosomal stalk of the 50S ribosomal subunit. Interacts with L10 and the large rRNA to form the base of the stalk. L10 forms an elongated spine to which L12 dimers bind in a sequential fashion forming a multimeric L10(L12)X complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323933.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3INI5"/>
    <dbReference type="SMR" id="Q3INI5"/>
    <dbReference type="STRING" id="348780.NP_4454A"/>
    <dbReference type="EnsemblBacteria" id="CAI50318">
        <property type="protein sequence ID" value="CAI50318"/>
        <property type="gene ID" value="NP_4454A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703121"/>
    <dbReference type="KEGG" id="nph:NP_4454A"/>
    <dbReference type="eggNOG" id="arCOG04372">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074237_4_0_2"/>
    <dbReference type="OrthoDB" id="8842at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00349">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.250">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1550.10">
        <property type="entry name" value="Ribosomal protein L11/L12, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00736">
        <property type="entry name" value="Ribosomal_uL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000911">
        <property type="entry name" value="Ribosomal_uL11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020783">
        <property type="entry name" value="Ribosomal_uL11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036769">
        <property type="entry name" value="Ribosomal_uL11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020785">
        <property type="entry name" value="Ribosomal_uL11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020784">
        <property type="entry name" value="Ribosomal_uL11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036796">
        <property type="entry name" value="Ribosomal_uL11_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002232">
        <property type="entry name" value="PRK01143.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL11M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03946">
        <property type="entry name" value="Ribosomal_L11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54747">
        <property type="entry name" value="Ribosomal L11/L12e N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46906">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00359">
        <property type="entry name" value="RIBOSOMAL_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000258250" description="Large ribosomal subunit protein uL11">
        <location>
            <begin position="1"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="163" mass="17129" checksum="44FCCD9ABEB1AA6A" modified="2005-11-08" version="1">MAGTIEVLVAGGQADPGPPLGPELGPTPVDVQAVVNEINDQTEAFDGTEVPVTIDYDDDGSFDIDVGVPPTAALIKDELDFETGSGEPNEEFVADMSAEQLKTVAEQKLPDLLAYDTRNAAKEVAGTCVSLGVTIEGEDARTFNQRLDNGEFDDVFAEAEPAA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IP72</accession>
    <name>PROA_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Gamma-glutamyl phosphate reductase</fullName>
            <shortName evidence="1">GPR</shortName>
            <ecNumber evidence="1">1.2.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glutamate-5-semialdehyde dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glutamyl-gamma-semialdehyde dehydrogenase</fullName>
            <shortName evidence="1">GSA dehydrogenase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">proA</name>
        <name type="ordered locus">NP_3978A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NADPH-dependent reduction of L-glutamate 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamate 5-semialdehyde + phosphate + NADP(+) = L-glutamyl 5-phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19541"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="ChEBI" id="CHEBI:58274"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.41"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the gamma-glutamyl phosphate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.41" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323696.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IP72"/>
    <dbReference type="SMR" id="Q3IP72"/>
    <dbReference type="STRING" id="348780.NP_3978A"/>
    <dbReference type="EnsemblBacteria" id="CAI50080">
        <property type="protein sequence ID" value="CAI50080"/>
        <property type="gene ID" value="NP_3978A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703054"/>
    <dbReference type="KEGG" id="nph:NP_3978A"/>
    <dbReference type="eggNOG" id="arCOG01253">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030231_0_1_2"/>
    <dbReference type="OrthoDB" id="53031at2157"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00360"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004350">
        <property type="term" value="F:glutamate-5-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07079">
        <property type="entry name" value="ALDH_F18-19_ProA-GPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000006">
        <property type="entry name" value="Gamma-glutamyl phosphate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00412">
        <property type="entry name" value="ProA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020593">
        <property type="entry name" value="G-glutamylP_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012134">
        <property type="entry name" value="Glu-5-SA_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000965">
        <property type="entry name" value="GPR_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001221">
        <property type="entry name" value="PRK00197.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00407">
        <property type="entry name" value="proA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11063:SF8">
        <property type="entry name" value="DELTA-1-PYRROLINE-5-CARBOXYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11063">
        <property type="entry name" value="GLUTAMATE SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000151">
        <property type="entry name" value="GPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01223">
        <property type="entry name" value="PROA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0641">Proline biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000230035" description="Gamma-glutamyl phosphate reductase">
        <location>
            <begin position="1"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00412"/>
        </source>
    </evidence>
    <sequence length="438" mass="47559" checksum="BB01CE7C2483D92F" modified="2005-11-08" version="1">MTETTEAKVDAAQTAALELANESDEARQENLQAIADAIDERRAEVLEANEKDVEAAEEMLEAGEYSQALVDRLKLSDAKLDSIIEMVRSVAGQEDPLGKTLTARELDDGLDLYKVSVPIGVIGTVFESRPDALVQIAALSLRSGNAVILKGGSEASHSNRVLYEIIREATADLPDGWAQLIEAREDVDRLLGMDDSVDLLMPRGSSAFVSYIQDNTSIPVLGHTEGVCHVYVDDAADLDMATDIAYDAKVQYPAVCNAVETLLVHEDVAEEYLPDIAARYAEADVEMRGDEATRSVLDRDIEAATDDDWTSEYGDLIVAIKVVDSLESAIDHINTNGSKHTESIVTEDDGRASTFMRRLDSASVFHNASTRFSDGYRFGLGAEVGISTGKIHARGPVGLKGLTTYKYHLEGDGHLVATYAGEDAKPFSHEEFDGEWSP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-05-20" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IPI7</accession>
    <name>MOAC_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable cyclic pyranopterin monophosphate synthase</fullName>
            <ecNumber evidence="1">4.6.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Molybdenum cofactor biosynthesis protein C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">moaC</name>
        <name type="ordered locus">NP_3746A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate to cyclic pyranopterin monophosphate (cPMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate = cyclic pyranopterin phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:49580"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59648"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.6.1.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homohexamer; trimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MoaC family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.17" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323581.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IPI7"/>
    <dbReference type="SMR" id="Q3IPI7"/>
    <dbReference type="STRING" id="348780.NP_3746A"/>
    <dbReference type="EnsemblBacteria" id="CAI49964">
        <property type="protein sequence ID" value="CAI49964"/>
        <property type="gene ID" value="NP_3746A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702925"/>
    <dbReference type="KEGG" id="nph:NP_3746A"/>
    <dbReference type="eggNOG" id="arCOG01530">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074693_1_2_2"/>
    <dbReference type="OrthoDB" id="10067at2157"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061799">
        <property type="term" value="F:cyclic pyranopterin monophosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.640">
        <property type="entry name" value="Molybdopterin cofactor biosynthesis C (MoaC) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01224_A">
        <property type="entry name" value="MoaC_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023047">
        <property type="entry name" value="Mo_CF_biosynth-C_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023045">
        <property type="entry name" value="MoaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036522">
        <property type="entry name" value="MoaC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050105">
        <property type="entry name" value="MoCo_biosynth_MoaA/MoaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002820">
        <property type="entry name" value="Mopterin_CF_biosynth-C_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00581">
        <property type="entry name" value="moaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008999">
        <property type="entry name" value="PRK12343.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960">
        <property type="entry name" value="MOLYBDOPTERIN COFACTOR SYNTHESIS PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01967">
        <property type="entry name" value="MoaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55040">
        <property type="entry name" value="Molybdenum cofactor biosynthesis protein C, MoaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000085677" description="Probable cyclic pyranopterin monophosphate synthase">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="78"/>
            <end position="80"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="114"/>
            <end position="115"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="160" mass="17116" checksum="F226681E63C3B0BC" modified="2005-11-08" version="1">MSDDSELTHVTDDGDAQMVDVGEKPDTARRAVARGEITLQPSTVAAIETNDVEKGDVLAVARVGAIQAVKHTWETVPMCHQIPITNVDTEFSTSETAVELTVAVETTGKTGCEMEALEGVTTGLNVVWDMVKAAEKDADGQYPDTGIDSVEVIEKEKRAL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-11-28" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IQT4</accession>
    <name>RL13_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL13</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L13</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl13</name>
        <name type="ordered locus">NP_2838A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323135.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IQT4"/>
    <dbReference type="SMR" id="Q3IQT4"/>
    <dbReference type="STRING" id="348780.NP_2838A"/>
    <dbReference type="EnsemblBacteria" id="CAI49510">
        <property type="protein sequence ID" value="CAI49510"/>
        <property type="gene ID" value="NP_2838A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703123"/>
    <dbReference type="KEGG" id="nph:NP_2838A"/>
    <dbReference type="eggNOG" id="arCOG04242">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076922_1_0_2"/>
    <dbReference type="OrthoDB" id="7668at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00392">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1180.10">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01366">
        <property type="entry name" value="Ribosomal_uL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005822">
        <property type="entry name" value="Ribosomal_uL13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005823">
        <property type="entry name" value="Ribosomal_uL13_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023563">
        <property type="entry name" value="Ribosomal_uL13_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005755">
        <property type="entry name" value="Ribosomal_uL13_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036899">
        <property type="entry name" value="Ribosomal_uL13_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01077">
        <property type="entry name" value="L13_A_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005004">
        <property type="entry name" value="PRK06394.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545">
        <property type="entry name" value="RIBOSOMAL PROTEIN L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00572">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002181">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52161">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00783">
        <property type="entry name" value="RIBOSOMAL_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000261846" description="Large ribosomal subunit protein uL13">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="144" mass="16294" checksum="69B1E92359C8CEC9" modified="2005-11-08" version="1">MSYAEFDADVVIDARDCILGRVASQVAERALDGERIAVVNAEQAVITGSEDDIMEVYRKRDEVGSDRGPRYPKRPDRIFKRSVRGMLPYKTTRGREAFENVRIYMGNPYEEAEVLEDTSLDRLSNIKFVSLGEVSENLGANVTW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IQT7</accession>
    <name>RS11_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein uS11</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">30S ribosomal protein S11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps11</name>
        <name type="ordered locus">NP_2832A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Located on the platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323132.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IQT7"/>
    <dbReference type="SMR" id="Q3IQT7"/>
    <dbReference type="STRING" id="348780.NP_2832A"/>
    <dbReference type="EnsemblBacteria" id="CAI49507">
        <property type="protein sequence ID" value="CAI49507"/>
        <property type="gene ID" value="NP_2832A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703166"/>
    <dbReference type="KEGG" id="nph:NP_2832A"/>
    <dbReference type="eggNOG" id="arCOG04240">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072439_6_1_2"/>
    <dbReference type="OrthoDB" id="12054at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022626">
        <property type="term" value="C:cytosolic ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044391">
        <property type="term" value="C:ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.80:FF:000018">
        <property type="entry name" value="40S ribosomal protein S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.80">
        <property type="entry name" value="Ribosomal protein S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01310">
        <property type="entry name" value="Ribosomal_uS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_uS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019961">
        <property type="entry name" value="Ribosomal_uS11_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018102">
        <property type="entry name" value="Ribosomal_uS11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036967">
        <property type="entry name" value="Ribosomal_uS11_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03628">
        <property type="entry name" value="arch_S11P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007176">
        <property type="entry name" value="PRK09607.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S14/30S RIBOSOMAL PROTEIN S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00411">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002131">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00054">
        <property type="entry name" value="RIBOSOMAL_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000230445" description="Small ribosomal subunit protein uS11">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="127" mass="13233" checksum="DC69DA69A7AD475F" modified="2005-11-08" version="1">MADDTTWGIANVYASFNNTIITVTDMTGAETIVKSSGGTVVKQNRDEASPYAAMQMAEVVAEDVKDAGIDGVHVRVRGPGGNQQQNPGPGAQATIRALARAGLEIGRIEDVTPIPHDGTRGPKNAGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IQY5</accession>
    <name>Q3IQY5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">o-succinylbenzoate synthase</fullName>
            <shortName evidence="4">OSB synthase</shortName>
            <shortName evidence="4">OSBS</shortName>
            <ecNumber evidence="4">4.2.1.113</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">4-(2'-carboxyphenyl)-4-oxybutyric acid synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">o-succinylbenzoic acid synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4 7" type="primary">menC</name>
        <name evidence="7" type="ordered locus">NP_2736A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Converts 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC) to 2-succinylbenzoate (OSB).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(1R,6R)-6-hydroxy-2-succinyl-cyclohexa-2,4-diene-1-carboxylate = 2-succinylbenzoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:10196"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18325"/>
            <dbReference type="ChEBI" id="CHEBI:58689"/>
            <dbReference type="EC" id="4.2.1.113"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Quinol/quinone metabolism; 1,4-dihydroxy-2-naphthoate biosynthesis; 1,4-dihydroxy-2-naphthoate from chorismate: step 4/7.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Quinol/quinone metabolism; menaquinone biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the mandelate racemase/muconate lactonizing enzyme family. MenC type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.113" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323084.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IQY5"/>
    <dbReference type="STRING" id="348780.NP_2736A"/>
    <dbReference type="EnsemblBacteria" id="CAI49459">
        <property type="protein sequence ID" value="CAI49459"/>
        <property type="gene ID" value="NP_2736A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702909"/>
    <dbReference type="KEGG" id="nph:NP_2736A"/>
    <dbReference type="eggNOG" id="arCOG01168">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030273_4_2_2"/>
    <dbReference type="OrthoDB" id="214520at2157"/>
    <dbReference type="UniPathway" id="UPA00079"/>
    <dbReference type="UniPathway" id="UPA01057">
        <property type="reaction ID" value="UER00165"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043748">
        <property type="term" value="F:O-succinylbenzoate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009063">
        <property type="term" value="P:amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009234">
        <property type="term" value="P:menaquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03320">
        <property type="entry name" value="OSBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00470">
        <property type="entry name" value="MenC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029065">
        <property type="entry name" value="Enolase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018110">
        <property type="entry name" value="Mandel_Rmase/mucon_lact_enz_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013342">
        <property type="entry name" value="Mandelate_racemase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010196">
        <property type="entry name" value="OSB_synthase_MenC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041338">
        <property type="entry name" value="OSBS_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48073:SF2">
        <property type="entry name" value="O-SUCCINYLBENZOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48073">
        <property type="entry name" value="O-SUCCINYLBENZOATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21508">
        <property type="entry name" value="MenC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13378">
        <property type="entry name" value="MR_MLE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00180">
        <property type="entry name" value="muconate_cycloisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00009">
        <property type="entry name" value="o-succinylbenzoate_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00922">
        <property type="entry name" value="MR_MLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00909">
        <property type="entry name" value="MR_MLE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0474">Menaquinone biosynthesis</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mandelate racemase/muconate lactonizing enzyme C-terminal" evidence="6">
        <location>
            <begin position="124"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="311"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="246"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="334" mass="34258" checksum="92FD47E1DB0F55EC" modified="2005-11-08" version="1">MDIQPFSLPLSSPLSTADGTIESRRGFLVRTEIDGVAGVGEAAPLPGWTESYDACQAALCGVEKPKQALEDNVFDDTPAARHAVSLAVADAAARRAGVPLYRRLGAGERVESVPVNATVGDGSPSATAEAVADAAAAGFPAAKVKVGNRSLDADIERIEAVRQRYPDIELRVDANGAWEFETAREAVESLSALGVAVLEQPLPAEALDNHRRLRGHGIDIALDEGLLRHGVDAAIAAEAADCLVCKPMALGGVDIARSAAERARADGWDVIVTTTIDGVVARAAAVHLAASVPDVRPCGLATGGRLASDVGPDAAPVSDGTAAVPQGKGNIPPL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-09-05" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IS99</accession>
    <name>SYP_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Proline--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Prolyl-tRNA synthetase</fullName>
            <shortName evidence="1">ProRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">proS</name>
        <name type="ordered locus">NP_1796A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Pro) + L-proline + ATP = L-prolyl-tRNA(Pro) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14305"/>
            <dbReference type="Rhea" id="RHEA-COMP:9700"/>
            <dbReference type="Rhea" id="RHEA-COMP:9702"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78532"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.15"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.15" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322621.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IS99"/>
    <dbReference type="SMR" id="Q3IS99"/>
    <dbReference type="STRING" id="348780.NP_1796A"/>
    <dbReference type="EnsemblBacteria" id="CAI48989">
        <property type="protein sequence ID" value="CAI48989"/>
        <property type="gene ID" value="NP_1796A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703057"/>
    <dbReference type="KEGG" id="nph:NP_1796A"/>
    <dbReference type="eggNOG" id="arCOG00402">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001882_4_2_2"/>
    <dbReference type="OrthoDB" id="7375at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004827">
        <property type="term" value="F:proline-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006433">
        <property type="term" value="P:prolyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00862">
        <property type="entry name" value="ProRS_anticodon_zinc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00778">
        <property type="entry name" value="ProRS_core_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000037">
        <property type="entry name" value="Proline--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.30">
        <property type="entry name" value="C-terminal domain of ProRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01571">
        <property type="entry name" value="Pro_tRNA_synth_type3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002316">
        <property type="entry name" value="Pro-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004499">
        <property type="entry name" value="Pro-tRNA-ligase_IIa_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016061">
        <property type="entry name" value="Pro-tRNA_ligase_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017449">
        <property type="entry name" value="Pro-tRNA_synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033721">
        <property type="entry name" value="ProRS_core_arch_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00408">
        <property type="entry name" value="proS_fam_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43382:SF2">
        <property type="entry name" value="BIFUNCTIONAL GLUTAMATE_PROLINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43382">
        <property type="entry name" value="PROLYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09180">
        <property type="entry name" value="ProRS-C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01046">
        <property type="entry name" value="TRNASYNTHPRO"/>
    </dbReference>
    <dbReference type="SMART" id="SM00946">
        <property type="entry name" value="ProRS-C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64586">
        <property type="entry name" value="C-terminal domain of ProRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000249166" description="Proline--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01571"/>
        </source>
    </evidence>
    <sequence length="482" mass="54269" checksum="615F741B85F9EF52" modified="2005-11-08" version="1">MTDQELGITESKEHNPGEWYAEVVQKAGLADYAPMGGFIVTRPRGYALWERLQDHLDGWFKETGVQNTYFPMFIPESYLEREKDIVDGFDPEVAWVTHGGHDELEERLAVRPTSESIITPFIAEWVRSYRDLPLRVNQWCSVVRWEATETKPFFRTKEFLWQEGHTAHATEESAWDETMTRLEQYERLYEEVMAIPGMTGRKPEHDKFPGADTTTTIEALMPDGKSVQAGTSHYLGTSFAEAFDIEYTDEDETKQTAHTTSWGLSWRALGALIMTHSDDQGLVIPPALAPTQVVVVPIWQADTEEAVKEYAADLAAELDEQFRVELDDRDERNPGFKFNEHELQGVPLRIEIGPNEVEDEAATLVHRPDGESDVAERESITDAVDEALETVYAKLYASAEATLEENIRKAHGRGEILGTLGQHGGYVKTGWCGDEACEAEIKDEIAAEIVMLPLDEDEPPVYDTCGVCGDEATETAYFAKSY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2026-01-28" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ITC8</accession>
    <name>AATA_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">A-type ATP synthase subunit A</fullName>
            <ecNumber evidence="1">7.1.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpA</name>
        <name type="ordered locus">NP_1030A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane. The A chain is the catalytic subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + 4 H(+)(in) = ADP + phosphate + 5 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Has multiple subunits with at least A(3), B(3), C, D, E, F, H, I and proteolipid K(x).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322241.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ITC8"/>
    <dbReference type="SMR" id="Q3ITC8"/>
    <dbReference type="STRING" id="348780.NP_1030A"/>
    <dbReference type="EnsemblBacteria" id="CAI48606">
        <property type="protein sequence ID" value="CAI48606"/>
        <property type="gene ID" value="NP_1030A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702538"/>
    <dbReference type="KEGG" id="nph:NP_1030A"/>
    <dbReference type="eggNOG" id="arCOG00868">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008162_3_1_2"/>
    <dbReference type="OrthoDB" id="115235at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033178">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, catalytic domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18111">
        <property type="entry name" value="ATP-synt_V_A-type_alpha_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01134">
        <property type="entry name" value="V_A-ATPase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000675">
        <property type="entry name" value="V-type ATP synthase alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1140.10:FF:000002">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000008">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1140.10">
        <property type="entry name" value="Bovine Mitochondrial F1-atpase, Atp Synthase Beta Chain, Chain D, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00309">
        <property type="entry name" value="ATP_synth_A_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031686">
        <property type="entry name" value="ATP-synth_a_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005726">
        <property type="entry name" value="ATP_synth_asu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024034">
        <property type="entry name" value="ATPase_F1/V1_b/a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022878">
        <property type="entry name" value="V-ATPase_asu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01043">
        <property type="entry name" value="ATP_syn_A_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003220">
        <property type="entry name" value="PRK04192.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607:SF1">
        <property type="entry name" value="H(+)-TRANSPORTING TWO-SECTOR ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607">
        <property type="entry name" value="V-TYPE PROTON ATPASE CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16886">
        <property type="entry name" value="ATP-synt_ab_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47917">
        <property type="entry name" value="C-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="N-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_1000059348" description="A-type ATP synthase subunit A">
        <location>
            <begin position="1"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="237"/>
            <end position="244"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00309"/>
        </source>
    </evidence>
    <sequence length="585" mass="65070" checksum="7586B161EFA2C1B1" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IU57</accession>
    <name>HIS2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribosyl-ATP pyrophosphatase</fullName>
            <shortName evidence="1">PRA-PH</shortName>
            <ecNumber evidence="1">3.6.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisE</name>
        <name type="ordered locus">NP_0470A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-(5-phospho-beta-D-ribosyl)-ATP + H2O = 1-(5-phospho-beta-D-ribosyl)-5'-AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22828"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59457"/>
            <dbReference type="ChEBI" id="CHEBI:73183"/>
            <dbReference type="EC" id="3.6.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PRA-PH family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.31" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321962.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IU57"/>
    <dbReference type="SMR" id="Q3IU57"/>
    <dbReference type="STRING" id="348780.NP_0470A"/>
    <dbReference type="EnsemblBacteria" id="CAI48326">
        <property type="protein sequence ID" value="CAI48326"/>
        <property type="gene ID" value="NP_0470A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702818"/>
    <dbReference type="KEGG" id="nph:NP_0470A"/>
    <dbReference type="eggNOG" id="arCOG02677">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123337_0_0_2"/>
    <dbReference type="OrthoDB" id="39686at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00007"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004636">
        <property type="term" value="F:phosphoribosyl-ATP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11534">
        <property type="entry name" value="NTP-PPase_HisIE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1080:FF:000002">
        <property type="entry name" value="Histidine biosynthesis bifunctional protein HisIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1080">
        <property type="entry name" value="MazG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01020">
        <property type="entry name" value="HisE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008179">
        <property type="entry name" value="HisE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021130">
        <property type="entry name" value="PRib-ATP_PPHydrolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03188">
        <property type="entry name" value="histidine_hisI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42945">
        <property type="entry name" value="HISTIDINE BIOSYNTHESIS BIFUNCTIONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42945:SF9">
        <property type="entry name" value="HISTIDINE BIOSYNTHESIS BIFUNCTIONAL PROTEIN HISIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01503">
        <property type="entry name" value="PRA-PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101386">
        <property type="entry name" value="all-alpha NTP pyrophosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000230198" description="Phosphoribosyl-ATP pyrophosphatase">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01020"/>
        </source>
    </evidence>
    <sequence length="101" mass="11386" checksum="7C9DE5CF9BF812DD" modified="2005-11-08" version="1">MSGDGHDGDEVLDELFEVIESRKEELPDGSYTASLFTHEKGENAVLEKLGEETTELLLAAKDDDDEELAHEAADIVYHLLVLLSMKDMELADLRAELRKRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IUM8</accession>
    <name>RS12_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein uS12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">30S ribosomal protein S12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps12</name>
        <name type="ordered locus">NP_0122A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">With S4 and S5 plays an important role in translational accuracy. Located at the interface of the 30S and 50S subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS12 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321791.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IUM8"/>
    <dbReference type="SMR" id="Q3IUM8"/>
    <dbReference type="STRING" id="348780.NP_0122A"/>
    <dbReference type="EnsemblBacteria" id="CAI48152">
        <property type="protein sequence ID" value="CAI48152"/>
        <property type="gene ID" value="NP_0122A"/>
    </dbReference>
    <dbReference type="GeneID" id="55900803"/>
    <dbReference type="KEGG" id="nph:NP_0122A"/>
    <dbReference type="eggNOG" id="arCOG04255">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115574_0_1_2"/>
    <dbReference type="OrthoDB" id="45154at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03367">
        <property type="entry name" value="Ribosomal_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000007">
        <property type="entry name" value="40S ribosomal protein S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00403_A">
        <property type="entry name" value="Ribosomal_uS12_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006032">
        <property type="entry name" value="Ribosomal_uS12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022863">
        <property type="entry name" value="Ribosomal_uS12_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005680">
        <property type="entry name" value="Ribosomal_uS12_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003254">
        <property type="entry name" value="PRK04211.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00982">
        <property type="entry name" value="uS12_E_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11652">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S12 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00164">
        <property type="entry name" value="Ribosom_S12_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002133">
        <property type="entry name" value="Ribosomal_S12/S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00055">
        <property type="entry name" value="RIBOSOMAL_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000226428" description="Small ribosomal subunit protein uS12">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="11"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="142" mass="15368" checksum="5C4866FFEAAF50F1" modified="2005-11-08" version="1">MANGKYAARKLKQDRQKHRWSDSDYARRARGLGKKSDPLEGAPQGRGIVLEKVGIEAKQPNSAIRKCVRVQLIKNGKQVTAFCPGDGAISFIDEHDEVTIAGIGGAKGRAMGDLSGVNYKVEKVNGVSLIELVRGNAEKPVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETF1</accession>
    <name>A0A1U7ETF1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Probable secreted glycoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0156A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321808.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETF1"/>
    <dbReference type="STRING" id="348780.NP_0156A"/>
    <dbReference type="EnsemblBacteria" id="CAI48169">
        <property type="protein sequence ID" value="CAI48169"/>
        <property type="gene ID" value="NP_0156A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700776"/>
    <dbReference type="KEGG" id="nph:NP_0156A"/>
    <dbReference type="eggNOG" id="arCOG02911">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099716_0_0_2"/>
    <dbReference type="OrthoDB" id="282668at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055713">
        <property type="entry name" value="DUF7289"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23960">
        <property type="entry name" value="DUF7289"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="235" mass="24402" checksum="D0B9109E43BE778F" modified="2017-05-10" version="1">MSRRAQSNVVGVALLLGIAVVSMGALTATVGMLVDSNAAAADAERVAADFDATFEPVAATGPQRGTVTFGEGRLTTVDRTVEIRVDGVTVETVQADALVYESDDRRVAFHSGAVVRGQAGEAWLETPPPITVDDDLIIVGVARLGDDIRTVSGSGGVTTTVRTNVSHSRTDLGTATVSVAVETAAPGAWERYFERHGASVDRRSGDPPTVEATFDGDRTGYLVVHDLAAEVGGHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETH1</accession>
    <name>A0A1U7ETH1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">MgtE family transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">tp48a</name>
        <name evidence="11" type="ordered locus">NP_0224A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC41A transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321842.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETH1"/>
    <dbReference type="STRING" id="348780.NP_0224A"/>
    <dbReference type="EnsemblBacteria" id="CAI48203">
        <property type="protein sequence ID" value="CAI48203"/>
        <property type="gene ID" value="NP_0224A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703325"/>
    <dbReference type="KEGG" id="nph:NP_0224A"/>
    <dbReference type="eggNOG" id="arCOG00624">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111003_1_0_2"/>
    <dbReference type="OrthoDB" id="86118at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.20">
        <property type="entry name" value="SLC41 divalent cation transporters, integral membrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006667">
        <property type="entry name" value="SLC41_membr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036739">
        <property type="entry name" value="SLC41_membr_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045349">
        <property type="entry name" value="SLC41A1-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16228">
        <property type="entry name" value="DIVALENT CATION TRANSPORTER SOLUTE CARRIER FAMILY 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16228:SF7">
        <property type="entry name" value="SLC41A_MGTE INTEGRAL MEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01769">
        <property type="entry name" value="MgtE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161093">
        <property type="entry name" value="MgtE membrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="14"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="44"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="88"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="126"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="164"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="SLC41A/MgtE integral membrane" evidence="10">
        <location>
            <begin position="52"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI48203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="192" mass="20327" checksum="910BC13613CC1D08" modified="2017-05-10" version="1">MDARHGAWRIYRESIAILVVSLGGGIFAGSVLGTEGMTDGFEQFPGLLLLLPAFLGTRGNVYGALGARISSGLHQGLIEPRFEWDRRLINAVTASFINGIGISVFIGFLSWVVIGFLRPESEASLLALVSIMLIAGVLTSVVLIVGLLALVFAGYKYGMDPDNLVGPVVTTLGDIFGVVFLYTAVLVVGVLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETI9</accession>
    <name>A0A1U7ETI9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">UbiA family prenyltransferase</fullName>
            <ecNumber evidence="6">2.5.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ubiA3</name>
        <name evidence="6" type="ordered locus">NP_0262A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.5.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321861.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETI9"/>
    <dbReference type="STRING" id="348780.NP_0262A"/>
    <dbReference type="EnsemblBacteria" id="CAI48222">
        <property type="protein sequence ID" value="CAI48222"/>
        <property type="gene ID" value="NP_0262A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703435"/>
    <dbReference type="KEGG" id="nph:NP_0262A"/>
    <dbReference type="eggNOG" id="arCOG00481">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077871_2_0_2"/>
    <dbReference type="OrthoDB" id="293340at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13967">
        <property type="entry name" value="PT_UbiA_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050475">
        <property type="entry name" value="Prenyltransferase_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42723">
        <property type="entry name" value="CHLOROPHYLL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42723:SF1">
        <property type="entry name" value="CHLOROPHYLL SYNTHASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="116"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="139"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="209"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="263"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI48222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="286" mass="30973" checksum="929F34606BEB2796" modified="2017-05-10" version="1">MERGTAGTRQLRRRLRDVAVLFVHSNLLISLAATSVAFSTMWLVGVSADPVPLFIVFAATLFVYSLNRLTDIEEDRQNVPRRAAFIERYGKPLFAAGVALYLGAIAVAVWLGLPGAPFLILPAVVAALYSTFKIKQRLLVKNLVVGVAWGIIPLGVGVYYGVLWTPEVLFAFVFVTVMLTVAAAIFDIKDIEGDRAEGIRTFPIVFSPAATRWGAAVITVLVAAGVVVAVATGVVSHRALVFLSLSAYVFAYIPFASRDRGPLFYGFVVDGEHVFLGAVVLLVEWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETL2</accession>
    <name>A0A1U7ETL2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glycine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Diadenosine tetraphosphate synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">glyS</name>
        <name evidence="12" type="ordered locus">NP_0328A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.14" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321893.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETL2"/>
    <dbReference type="STRING" id="348780.NP_0328A"/>
    <dbReference type="EnsemblBacteria" id="CAI48255">
        <property type="protein sequence ID" value="CAI48255"/>
        <property type="gene ID" value="NP_0328A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702766"/>
    <dbReference type="KEGG" id="nph:NP_0328A"/>
    <dbReference type="eggNOG" id="arCOG00405">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015515_1_2_2"/>
    <dbReference type="OrthoDB" id="6113at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004820">
        <property type="term" value="F:glycine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006426">
        <property type="term" value="P:glycyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00774">
        <property type="entry name" value="GlyRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00858">
        <property type="entry name" value="GlyRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000002">
        <property type="entry name" value="Glycine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRNASYNTHGLY"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
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    </dbReference>
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        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
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    </dbReference>
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    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="5"/>
            <end position="483"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012829"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI48255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="578" mass="64345" checksum="897F1F616DCE4297" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETM1</accession>
    <name>A0A1U7ETM1_NATPD</name>
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        <submittedName>
            <fullName evidence="10">Kef-type transport system</fullName>
        </submittedName>
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        <name evidence="10" type="primary">kef3</name>
        <name evidence="10" type="synonym">tp40b</name>
        <name evidence="10" type="ordered locus">NP_0332A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
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        <lineage>
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            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
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            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETM1"/>
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    <dbReference type="EnsemblBacteria" id="CAI48257">
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        <property type="gene ID" value="NP_0332A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703317"/>
    <dbReference type="KEGG" id="nph:NP_0332A"/>
    <dbReference type="eggNOG" id="arCOG01955">
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    </dbReference>
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    <dbReference type="OrthoDB" id="43518at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:proton transmembrane transport"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="8">
        <location>
            <begin position="14"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="9">
        <location>
            <begin position="408"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF02254"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI48257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="553" mass="58536" checksum="B4FBC5AC88AD23E8" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETQ0</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0412A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <lineage>
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            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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    </dbReference>
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    <dbReference type="KEGG" id="nph:NP_0412A"/>
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    </dbReference>
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    <dbReference type="OrthoDB" id="157372at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
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    </dbReference>
    <dbReference type="InterPro" id="IPR043808">
        <property type="entry name" value="DUF5790"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19103">
        <property type="entry name" value="DUF5790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="80"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="130" mass="14144" checksum="11E06E413E1253C9" modified="2017-05-10" version="1">MSQATLDDDDLFDEAATEMREDVEASLDAAREELPAAADIWTTDAGNTLGVLNGLKESLDTGEAETHLRDAKKWFTMGRKADAFEDADDLETDIAALEALLEDIETAHEQVSDLTATIPELRSALDDSDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETU7</accession>
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        <submittedName>
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        </submittedName>
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        <name evidence="3" type="ordered locus">NP_0530A</name>
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    <organism evidence="3 4">
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
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            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48356.1"/>
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    </dbReference>
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    </dbReference>
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    <dbReference type="STRING" id="348780.NP_0530A"/>
    <dbReference type="EnsemblBacteria" id="CAI48356">
        <property type="protein sequence ID" value="CAI48356"/>
        <property type="gene ID" value="NP_0530A"/>
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    </dbReference>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY TRANSPORTER"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
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        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
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        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
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        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
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        <location>
            <begin position="186"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="302"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="334"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="358"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="397"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="422"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="2">
        <location>
            <begin position="21"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="450" mass="46978" checksum="C7C02F49F072B5E9" modified="2017-05-10" version="1">MGSGPTGLRGTGRQLLALERDVLVLSVAMFAFSLGFQMTSRYIPRYMSVLGAGAVAIGLFGAFGNLISALYPYPGGAVSDRIGSRTALTLFGLLSTAGFLIWLFADAFGTVVVPPVGTEREVFGREVVIGWLEPAVVPVGIFLGLVFAQAWKSFGLGATFAIVKQSVSPDQLATGFASTETFRRTAFLLGPLLAAGILAVFTFEVGFRIVLAVAAAFGLVATLAQHFLYDASEDTLGKSFAGVSAVVDDLRSMPSELRPLLVADTLVRFGNGMVYVFFIIVVTEFLEVGVSLPVVGRLSPDAYFGVLLAIEMLVALLIMLPVSRLSRRVGLKPVVAVGFAVYAIFPAVLIYAPDNAAVLALLFAVSGLRFAGLPAHKALIVGPAESGSGGRVTGSYYLVRNVVVIPSAAIGGAIYAVSPETAFLTATAIGLAGLVVFLAFGREFEAAVAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETX2</accession>
    <name>A0A1U7ETX2_NATPD</name>
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        <submittedName>
            <fullName evidence="2">Origin-associated protein OapC</fullName>
        </submittedName>
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        <name evidence="2" type="primary">oapC</name>
        <name evidence="2" type="ordered locus">NP_0590A</name>
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        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48386.1"/>
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    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A1U7ETX2"/>
    <dbReference type="STRING" id="348780.NP_0590A"/>
    <dbReference type="EnsemblBacteria" id="CAI48386">
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        <property type="gene ID" value="NP_0590A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700898"/>
    <dbReference type="KEGG" id="nph:NP_0590A"/>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063606_0_0_2"/>
    <dbReference type="OrthoDB" id="78050at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="entry name" value="OapC-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09845">
        <property type="entry name" value="OapC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="114"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="161"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="215"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="326" mass="33918" checksum="FC8A79E3387A3B25" modified="2017-05-10" version="1">MPHQCTGCGHTFEDGSKEMLSGCPECGGNKFQFRPSEEALSDPTDGDSSPPDEEPPEPPAADSTTSSVAETVGRATTRVRDLVSSSEQPAPDETAGTTNTASDAEAPTGDDLETPSSSSASTEPAAGTAGSDTDSNSGSGPDPEAEWPGNWSGSDSDASEAGESPPSGASDPSASSGDDARDSEILDDSAGENSAQATARSDVVSPDELNAVARSDPSPTDTDTTASPPEEPVSHDEPSASSDTPPEADADTTSASSPDDDQPDLAALREQLNEQFESIKIVEPGQYELNLMELYDREEYIIALQENGRYVIQVPEQWMGDDPDER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETX3</accession>
    <name>A0A1U7ETX3_NATPD</name>
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        <submittedName>
            <fullName evidence="3">Cupin 2 barrel domain protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ordered locus">NP_0572A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
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            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAI48377.1"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETX3"/>
    <dbReference type="STRING" id="348780.NP_0572A"/>
    <dbReference type="EnsemblBacteria" id="CAI48377">
        <property type="protein sequence ID" value="CAI48377"/>
        <property type="gene ID" value="NP_0572A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700892"/>
    <dbReference type="KEGG" id="nph:NP_0572A"/>
    <dbReference type="eggNOG" id="arCOG02998">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="190812at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051610">
        <property type="entry name" value="GPI/OXD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35848">
        <property type="entry name" value="OXALATE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35848:SF9">
        <property type="entry name" value="SLL1358 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin type-2" evidence="2">
        <location>
            <begin position="35"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="155" mass="17040" checksum="DF2A870C59995AF4" modified="2017-05-10" version="1">METVDIDDIDSWMGPASVKRPVGKALGAEELALNYYELAPGESFAFGYHAHEKQEEVFYVLDGTVTFETDDGEVVVEDGEAIYFEAGEFQRGVNESDERVRALAVGAPAETEELTLLRACESCDGRTEQEIEPTADRDALITRCVDCGTETGRFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETX7</accession>
    <name>A0A1U7ETX7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0598A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="CAI48390">
        <property type="protein sequence ID" value="CAI48390"/>
        <property type="gene ID" value="NP_0598A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700901"/>
    <dbReference type="KEGG" id="nph:NP_0598A"/>
    <dbReference type="eggNOG" id="arCOG04737">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1063980_0_0_2"/>
    <dbReference type="OrthoDB" id="198543at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055515">
        <property type="entry name" value="DUF7089"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23363">
        <property type="entry name" value="DUF7089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="261" mass="28238" checksum="9E2C47FD9C58D576" modified="2017-05-10" version="1">MFERRALTDALSAVRDEYAPSALVVDAANDFETLPPSVAENLLAVVDGVEPLAYDETTVPPDAPDILHRLADSEFTVGAPGDGGVTWTRQTSPPTVIVKPRLEGSPEPFIDFLIAEALVELHLDSAEHFLGFFGDAYEDLNDAVPLSPVDTYQLAAALHTAHIGRRSRPVFDSWADQFPDIHAAWADAGERLEPRLADLSADVATGRTDFADAAELACNALKHDSAVPKPFDALDTEAYTDHGAPFAVRWAEKTFAELDTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU00</accession>
    <name>A0A1U7EU00_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Blp-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">blp</name>
        <name evidence="1" type="ordered locus">NP_0648A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48415.1"/>
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    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU00"/>
    <dbReference type="STRING" id="348780.NP_0648A"/>
    <dbReference type="EnsemblBacteria" id="CAI48415">
        <property type="protein sequence ID" value="CAI48415"/>
        <property type="gene ID" value="NP_0648A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702555"/>
    <dbReference type="KEGG" id="nph:NP_0648A"/>
    <dbReference type="eggNOG" id="arCOG02898">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136031_0_0_2"/>
    <dbReference type="OrthoDB" id="195304at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07812">
        <property type="entry name" value="SRPBCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019587">
        <property type="entry name" value="Polyketide_cyclase/dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10604">
        <property type="entry name" value="Polyketide_cyc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="141" mass="16036" checksum="B2EAC103205DB829" modified="2017-05-10" version="1">MKAEATTIVDRPRPAVFEFMDVPENQARISPRLTRVETLGTLDNGGKRASYTYRLFGSRFTGEVRGVVHEPPERIRFEMRGDIEGTIEWTFEPAEDGTRVTYSAEYDLGLPAVVEALCSPLVRRFNQREVEATLDSLRKQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU20</accession>
    <name>A0A1U7EU20_NATPD</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0688A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU20"/>
    <dbReference type="STRING" id="348780.NP_0688A"/>
    <dbReference type="EnsemblBacteria" id="CAI48435">
        <property type="protein sequence ID" value="CAI48435"/>
        <property type="gene ID" value="NP_0688A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700930"/>
    <dbReference type="KEGG" id="nph:NP_0688A"/>
    <dbReference type="eggNOG" id="arCOG06450">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1387561_0_0_2"/>
    <dbReference type="OrthoDB" id="296096at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055897">
        <property type="entry name" value="DUF7474"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24286">
        <property type="entry name" value="DUF7474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="198" mass="22912" checksum="DE51C359C1080E9F" modified="2017-05-10" version="1">MRLRYRCPGCRTTSNLHDPSCEFEGTDRTHIERAYTDLLAPLSARTYADEDALRDDVPTDRWSGLHTAVLRRLKSDQRIVEDDDGRLRLLPPDEYKDRVSRPTFEPLQTIYEQGSVPGAHDHSVFAIIAFYEMVGLSWEEAREQTIEWLHDSGTWNRGGFEEDTPEELVDSKKHVYEEGYGWKQAATEAKAVIDRRLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU31</accession>
    <name>A0A1U7EU31_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ArCOG06342 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0714A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322084.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU31"/>
    <dbReference type="STRING" id="348780.NP_0714A"/>
    <dbReference type="EnsemblBacteria" id="CAI48448">
        <property type="protein sequence ID" value="CAI48448"/>
        <property type="gene ID" value="NP_0714A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700939"/>
    <dbReference type="KEGG" id="nph:NP_0714A"/>
    <dbReference type="eggNOG" id="arCOG06342">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111886_0_0_2"/>
    <dbReference type="OrthoDB" id="178099at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.20">
        <property type="entry name" value="Apolipoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58113">
        <property type="entry name" value="Apolipoprotein A-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="123"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="220" mass="24405" checksum="BFBDFCF19AF6BDB3" modified="2017-05-10" version="1">MSDDSSPFTAVFELQRETIQQTEAALEEAASIPRDVGDALKSGTDVQRKVNERALDAVRQSVHTSLDAAESVTGNTDPVADLRDAVDETFDTLKEQQAEALDAVDEEYDEFGESYDEFSEEAIENLSAQLDTLVELNEGVEDQLGETLDELLDQVDELQSQFDASAEEAREQLSESTEAALDQLEAQLDGLADQFEAQAERFEELEDRIEDVSLEDDDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU74</accession>
    <name>A0A1U7EU74_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Small CPxCG-related zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0816A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322135.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU74"/>
    <dbReference type="EnsemblBacteria" id="CAI48499">
        <property type="protein sequence ID" value="CAI48499"/>
        <property type="gene ID" value="NP_0816A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700969"/>
    <dbReference type="KEGG" id="nph:NP_0816A"/>
    <dbReference type="eggNOG" id="arCOG06400">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_164491_0_0_2"/>
    <dbReference type="OrthoDB" id="311125at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055987">
        <property type="entry name" value="DUF7565"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24446">
        <property type="entry name" value="DUF7565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="1">
        <location>
            <begin position="2"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="1">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="101" mass="11557" checksum="B767923A408ADEAF" modified="2017-05-10" version="1">MWECAIDGCEYATDDVEDLLVHQAEAHGQHTCKVCGTTVPDGFFAIRHAFTEHSRAEYLRNYEADTDDIRHREKVAKEIQKQADIERVVDRIKSEPAEPQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU83</accession>
    <name>A0A1U7EU83_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">4-phosphopantoate--beta-alanine ligase</fullName>
            <ecNumber evidence="5">6.3.2.36</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Phosphopantothenate synthetase</fullName>
            <shortName evidence="5">PPS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">NP_0848A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the condensation of (R)-4-phosphopantoate and beta-alanine to 4'-phosphopantothenate in the CoA biosynthesis pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(R)-4-phosphopantoate + beta-alanine + ATP = (R)-4'-phosphopantothenate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27930"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57966"/>
            <dbReference type="ChEBI" id="CHEBI:61294"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.2.36"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the archaeal phosphopantothenate synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.36" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322151.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU83"/>
    <dbReference type="STRING" id="348780.NP_0848A"/>
    <dbReference type="EnsemblBacteria" id="CAI48515">
        <property type="protein sequence ID" value="CAI48515"/>
        <property type="gene ID" value="NP_0848A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700974"/>
    <dbReference type="KEGG" id="nph:NP_0848A"/>
    <dbReference type="eggNOG" id="arCOG04262">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078701_0_0_2"/>
    <dbReference type="OrthoDB" id="10078at2157"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016881">
        <property type="term" value="F:acid-amino acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12640">
        <property type="entry name" value="Phosphopantoate/pantothenate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02224">
        <property type="entry name" value="PPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002855">
        <property type="entry name" value="PPS/PS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038138">
        <property type="entry name" value="PPS/PS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041123">
        <property type="entry name" value="phpantohe_syn_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010324">
        <property type="entry name" value="PRK13761.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40695">
        <property type="entry name" value="4-PHOSPHOPANTOATE--BETA-ALANINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40695:SF1">
        <property type="entry name" value="4-PHOSPHOPANTOATE--BETA-ALANINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02006">
        <property type="entry name" value="PPS_PS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004853">
        <property type="entry name" value="UCP004853"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="1 5 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="182"/>
            <end position="184"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="188"/>
            <end position="189"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="200"/>
            <end position="201"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI48515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="246" mass="25879" checksum="535544BC84B84B52" modified="2017-05-10" version="1">MTGPDIPESHPRYESLLTRHRIEEGVDLGITSRQGLIAQGRGEAFDYLLGEETIPSADDAARAAAAHLLSADQPVLSVNGNVAALAPGEMVALAAATGADIEVNLFNRTEARMERIADHLREHGAEEVKGLNADGRIPGLDHERATVDADGIGSADVVLVPLEDGDRAAALAEMGKTELVIDLNPMSRSAQSAAVPIVDNLIRAVPNMTAHAEALAGEDASTLENIIDDFDADAALSAAEERIRDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUB1</accession>
    <name>A0A1U7EUB1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Helicase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_0898A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322176.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUB1"/>
    <dbReference type="STRING" id="348780.NP_0898A"/>
    <dbReference type="EnsemblBacteria" id="CAI48540">
        <property type="protein sequence ID" value="CAI48540"/>
        <property type="gene ID" value="NP_0898A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700993"/>
    <dbReference type="KEGG" id="nph:NP_0898A"/>
    <dbReference type="eggNOG" id="arCOG00770">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033880_1_0_2"/>
    <dbReference type="OrthoDB" id="76985at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006555">
        <property type="entry name" value="ATP-dep_Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045028">
        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010614">
        <property type="entry name" value="RAD3-like_helicase_DEAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="DNA REPAIR DEAD HELICASE RAD3/XP-D SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472:SF34">
        <property type="entry name" value="REGULATOR OF TELOMERE ELONGATION HELICASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06733">
        <property type="entry name" value="DEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="3">
        <location>
            <begin position="33"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-dependent helicase C-terminal" evidence="4">
        <location>
            <begin position="394"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="540"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="275"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI48540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="586" mass="64800" checksum="9F8C6DA654CB6D0E" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUC5</accession>
    <name>A0A1U7EUC5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Alpha/beta hydrolase fold protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_0926A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. AB hydrolase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322190.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUC5"/>
    <dbReference type="STRING" id="348780.NP_0926A"/>
    <dbReference type="ESTHER" id="natpd-q3ith9">
        <property type="family name" value="LYsophospholipase_carboxylesterase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI48554">
        <property type="protein sequence ID" value="CAI48554"/>
        <property type="gene ID" value="NP_0926A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701000"/>
    <dbReference type="KEGG" id="nph:NP_0926A"/>
    <dbReference type="eggNOG" id="arCOG06256">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049413_5_3_2"/>
    <dbReference type="OrthoDB" id="203477at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050565">
        <property type="entry name" value="LYPA1-2/EST-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003140">
        <property type="entry name" value="PLipase/COase/thioEstase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10655:SF17">
        <property type="entry name" value="LYSOPHOSPHOLIPASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10655">
        <property type="entry name" value="LYSOPHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02230">
        <property type="entry name" value="Abhydrolase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phospholipase/carboxylesterase/thioesterase" evidence="3">
        <location>
            <begin position="26"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="224" mass="24115" checksum="750FB431E895B698" modified="2017-05-10" version="1">MDTPQSDASAELPLVHVSRPGTVDSNAPAIVLIHGRGTNERDLLPLAAQLPDELHVLSVRAPQPMDGPDSYTWYDLDLSAGGLHASQPDPEGFRRSLDLVHDFVDAAIEAYDLDADRVGLLGFSQGAITSLSALLERPEAYRWIVALNGYLAEAHHDEVENADGTPVFVGCGNRDQVIPPERAQRAAELLGEGGAEVRFERYDVGHGTTPAAVTDVGGWLEGRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUC8</accession>
    <name>A0A1U7EUC8_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Metal-dependent carboxypeptidase</fullName>
            <ecNumber evidence="1">3.4.17.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cxp</name>
        <name evidence="4" type="ordered locus">NP_0944A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Broad specificity carboxypetidase that releases amino acids sequentially from the C-terminus, including neutral, aromatic, polar and basic residues.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of a C-terminal amino acid with broad specificity, except for -Pro.</text>
            <dbReference type="EC" id="3.4.17.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="2">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M32 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.17.19" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322199.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUC8"/>
    <dbReference type="STRING" id="348780.NP_0944A"/>
    <dbReference type="EnsemblBacteria" id="CAI48563">
        <property type="protein sequence ID" value="CAI48563"/>
        <property type="gene ID" value="NP_0944A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702645"/>
    <dbReference type="KEGG" id="nph:NP_0944A"/>
    <dbReference type="eggNOG" id="arCOG04247">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032916_1_1_2"/>
    <dbReference type="OrthoDB" id="7244at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06460">
        <property type="entry name" value="M32_Taq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1370.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001333">
        <property type="entry name" value="Peptidase_M32_Taq"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34217:SF1">
        <property type="entry name" value="CARBOXYPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34217">
        <property type="entry name" value="METAL-DEPENDENT CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02074">
        <property type="entry name" value="Peptidase_M32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006615">
        <property type="entry name" value="Zn_crbxpep_Taq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00998">
        <property type="entry name" value="CRBOXYPTASET"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52034">
        <property type="entry name" value="PEPTIDASE_M32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="1 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="273"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR006615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR006615-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR006615-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="506" mass="56529" checksum="82E4D10DE83F0D88" modified="2017-05-10" version="1">MTNPDSAGSDADASTPTAYDDLLERYRRATYLSDANGVLSWDQEVMMPEGGTPARSKQRGAVSAVAHDVLVDEETADALDALDGVDLTDDEAAVVREIRREHERKARVPTELVEEISTATSEAHPVWNEAKAADDFDAFAPTLAELLDLKRRYAEHIDPDADPYATLFAEYEPYLDLSTAERVLEELRDGLVPLIDAIGASDTELYALEGTFPEDDQFAVTEAALETLGYDFEHGRLDTAPHPFSTGTQFDARVTTRFDESEPLDSLASTIHEFGHATYTLGLPRDAYGSPLGEHRGLSVHESQSRLFENHVGRSKPFWELFVDRFNDHHDTELTPQQAYEAANRVYDDNLIRVEADELTYHLHIVLRFEIERELLAGDLDVEEVPAAWNDRMESYLGVRPETDAEGCLQDIHWSHGSFGYFPTYSLGSVLAAQLYAAADEAIDGLESQIAAGEFDALHEWLTENIHQHGRRYTTGDVIERATGEPLTADYFLEYAEAKFGELYDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUD6</accession>
    <name>A0A1U7EUD6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DsrE domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0976A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322214.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUD6"/>
    <dbReference type="STRING" id="348780.NP_0976A"/>
    <dbReference type="EnsemblBacteria" id="CAI48579">
        <property type="protein sequence ID" value="CAI48579"/>
        <property type="gene ID" value="NP_0976A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701017"/>
    <dbReference type="KEGG" id="nph:NP_0976A"/>
    <dbReference type="eggNOG" id="arCOG02064">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094970_1_0_2"/>
    <dbReference type="OrthoDB" id="69354at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032836">
        <property type="entry name" value="DsrE2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34655">
        <property type="entry name" value="CONSERVED WITHIN P. AEROPHILUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34655:SF2">
        <property type="entry name" value="PEROXIREDOXIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13686">
        <property type="entry name" value="DrsE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="26"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="202" mass="21760" checksum="C69A7423CC3944E5" modified="2017-05-10" version="1">MSTETPADAEPSEEPNDGTAVSPEAVAELQARIDDLETELADLRAEREADSDKMVIIATKGTLDMAYPPLILASTAAAFGYDVTVFHTFWGLDIVHEENSKNLKLSSVGNPNMPVPNAIAALPGMDRMTTRMMRGKIEANDVATVEELVRTSLDSGVDLQACQMTIDLLGYDEDDFYDGVTTGVGAASAFQEMQEADIQLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUD7</accession>
    <name>A0A1U7EUD7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UPF0033 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0978A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfur carrier protein TusA family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322215.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUD7"/>
    <dbReference type="STRING" id="348780.NP_0978A"/>
    <dbReference type="EnsemblBacteria" id="CAI48580">
        <property type="protein sequence ID" value="CAI48580"/>
        <property type="gene ID" value="NP_0978A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703208"/>
    <dbReference type="KEGG" id="nph:NP_0978A"/>
    <dbReference type="eggNOG" id="arCOG02062">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165255_1_1_2"/>
    <dbReference type="OrthoDB" id="45650at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00291">
        <property type="entry name" value="SirA_YedF_YeeD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.40">
        <property type="entry name" value="TusA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001455">
        <property type="entry name" value="TusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036868">
        <property type="entry name" value="TusA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33279">
        <property type="entry name" value="SULFUR CARRIER PROTEIN YEDF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33279:SF6">
        <property type="entry name" value="SULFUR CARRIER PROTEIN YEDF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01206">
        <property type="entry name" value="TusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64307">
        <property type="entry name" value="SirA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01148">
        <property type="entry name" value="UPF0033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0033" evidence="2">
        <location>
            <begin position="9"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="83" mass="8868" checksum="DA9B50CDE86A07C1" modified="2017-05-10" version="1">MSTDATETLDVKGENCPMPVIKTKGAFDDLDAGDTLEVVATDAGSLSDIEGWADSTDGAELLDQEEAEEDGAAVYKHYLRKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUH1</accession>
    <name>A0A1U7EUH1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1042A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322247.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUH1"/>
    <dbReference type="EnsemblBacteria" id="CAI48612">
        <property type="protein sequence ID" value="CAI48612"/>
        <property type="gene ID" value="NP_1042A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701035"/>
    <dbReference type="KEGG" id="nph:NP_1042A"/>
    <dbReference type="eggNOG" id="ENOG502N5KC">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_571897_0_0_2"/>
    <dbReference type="OrthoDB" id="202329at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="231"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="324"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="408"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="434"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="477" mass="49348" checksum="351715640C0FF4AF" modified="2017-05-10" version="1">MLNRIAEAVSVADDERSFRQRAGGWVASVRVFVGLLLLYELTVGGWWKLGAPQLAWPPFEPNPGWVGENAGEVLANAAAGRAIEEGTYSWYAALLEGVVLPYAGFWSVVAVVAQLAVGLAFVVGFWNRPAAVVGLLYFVPVFHFGTIRTSPLFGVPIAFLLVTRAGHHYGLDGLIAARSGRLAQLSDRIATLSVLPRPSRSVLPGAVAALSVLSVYYLLSVPGREVTRQALVGLEVAVMLGLVAGGLALYYRGGEPVAVAADMVRAFVGYRFLHEVFVRDHAGVNGLPGWASVDAQAELLAETIVPAHVGPVATAIETVVLPTLPFWVVVFAAVQTAVGAALLVGYRTRLAGTVGAGYLVVLIGLGFVRLAPLLFASALVAATLSGRYASLDAVAGRRPMPPRLRQQVAAPAAAGAAVLFAGGAILGIDPEAGYGAVVGPVALVMLAFVLAAIAVAAAGATKPAAESDPVPDAAATD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUI4</accession>
    <name>A0A1U7EUI4_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Replication factor C small subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Clamp loader small subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rfcC</name>
        <name evidence="8" type="ordered locus">NP_1068A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the activator 1 small subunits family. RfcS subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322261.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUI4"/>
    <dbReference type="STRING" id="348780.NP_1068A"/>
    <dbReference type="EnsemblBacteria" id="CAI48625">
        <property type="protein sequence ID" value="CAI48625"/>
        <property type="gene ID" value="NP_1068A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703102"/>
    <dbReference type="KEGG" id="nph:NP_1068A"/>
    <dbReference type="eggNOG" id="arCOG00469">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042324_2_1_2"/>
    <dbReference type="OrthoDB" id="301951at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049945">
        <property type="entry name" value="AAA_22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013748">
        <property type="entry name" value="Rep_factorC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009067">
        <property type="entry name" value="PRK12402.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF20">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13401">
        <property type="entry name" value="AAA_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08542">
        <property type="entry name" value="Rep_fac_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="32"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI48625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="332" mass="36460" checksum="8CA7DAA7DDEF6734" modified="2017-05-10" version="1">MDAPLWTETHAPALEALPQPEVRDHLDRAVAEPMNLVVFGPRGAGKTAAVRALAAATHEDPDNDFVVINVADFFDRTKKEIRNDDRFSQFLEGRSGLSKRDMISHVLKEQAAYQPVSGSFRTILLDNAEAIREDFQQALRRVMEKHYEATQFVIATRQPSKLIAPIESRCFPVPVRAPSHEETVSVLESIVEAEGVEYDADGLEYVAGYGDGDLRKAILGAQTAAEEAGEVTMQAAYDALGDIGLRERMEEILAAADSGDFEDARSDLDDLLYDDGYEGAAVLQELLAAARARYDGDELVGVYELAGEIDHEMAAGNTDRIHLSRLLAELGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUM2</accession>
    <name>A0A1U7EUM2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Phosphoenolpyruvate synthase</fullName>
            <shortName evidence="12">PEP synthase</shortName>
            <ecNumber evidence="12">2.7.9.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Pyruvate, water dikinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">ppsA</name>
        <name evidence="17" type="ordered locus">NP_1196A</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 12">Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>pyruvate + ATP + H2O = phosphoenolpyruvate + AMP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:11364"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.9.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the PEP-utilizing enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.7.9.2" evidence="12"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322325.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUM2"/>
    <dbReference type="STRING" id="348780.NP_1196A"/>
    <dbReference type="EnsemblBacteria" id="CAI48689">
        <property type="protein sequence ID" value="CAI48689"/>
        <property type="gene ID" value="NP_1196A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703048"/>
    <dbReference type="KEGG" id="nph:NP_1196A"/>
    <dbReference type="eggNOG" id="arCOG01111">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007308_6_2_2"/>
    <dbReference type="OrthoDB" id="23397at2157"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008986">
        <property type="term" value="F:pyruvate, water dikinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000010">
        <property type="entry name" value="Phosphoenolpyruvate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.10">
        <property type="entry name" value="Phosphohistidine domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008279">
        <property type="entry name" value="PEP-util_enz_mobile_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006319">
        <property type="entry name" value="PEP_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018274">
        <property type="entry name" value="PEP_util_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000121">
        <property type="entry name" value="PEP_util_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036637">
        <property type="entry name" value="Phosphohistidine_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002192">
        <property type="entry name" value="PPDK_AMP/ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01418">
        <property type="entry name" value="PEP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005057">
        <property type="entry name" value="PRK06464.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43030">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43030:SF1">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00391">
        <property type="entry name" value="PEP-utilizers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02896">
        <property type="entry name" value="PEP-utilizers_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01326">
        <property type="entry name" value="PPDK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000854">
        <property type="entry name" value="PEP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01736">
        <property type="entry name" value="PHPHTRNFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52009">
        <property type="entry name" value="Phosphohistidine domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00370">
        <property type="entry name" value="PEP_ENZYMES_PHOS_SITE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12 17" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate phosphate dikinase AMP/ATP-binding" evidence="15">
        <location>
            <begin position="26"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilising enzyme mobile" evidence="14">
        <location>
            <begin position="369"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilising enzyme C-terminal" evidence="16">
        <location>
            <begin position="458"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="430"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="443"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR000854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAI48689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="759" mass="82519" checksum="36CAC5E977921682" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUM9</accession>
    <name>A0A1U7EUM9_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">NP_1154A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322304.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUM9"/>
    <dbReference type="STRING" id="348780.NP_1154A"/>
    <dbReference type="EnsemblBacteria" id="CAI48668">
        <property type="protein sequence ID" value="CAI48668"/>
        <property type="gene ID" value="NP_1154A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701069"/>
    <dbReference type="KEGG" id="nph:NP_1154A"/>
    <dbReference type="eggNOG" id="arCOG06408">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_58_2"/>
    <dbReference type="OrthoDB" id="3369at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013656">
        <property type="entry name" value="PAS_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052162">
        <property type="entry name" value="Sensor_kinase/Photoreceptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43304:SF1">
        <property type="entry name" value="PAC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43304">
        <property type="entry name" value="PHYTOCHROME-LIKE PROTEIN CPH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08448">
        <property type="entry name" value="PAS_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAC" evidence="9">
        <location>
            <begin position="105"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="8">
        <location>
            <begin position="152"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="9">
        <location>
            <begin position="226"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="288"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI48668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="495" mass="54651" checksum="DCED9573CF0C0BE3" modified="2017-05-10" version="1">MAEETGGTEGGGTGAEDDNPKTGLGKMEYETVFKAVEDAVFLFDVERDGDGEPRFVFRGGNPSHERATEMEADGCLGTPLRELFDGDGERLLDEYRRCVETEETVTYETTLEHGTGSVEWRTKITPVVEGGTATSIVGVARDVTERNKRRTELQRKEAMLQHTTDVVLLVDENGRIRYQNHADEAVLGHSTRDLTGASVTEYIHPEDSDGVEAMLAEALDSPEEVISGEHRYRTPDGEWRWYETRVRSLLDTPPIEGFLVSARDITERKAYEERLESQRSDLEVLNEVVRHDIRNDLQLVTAYAEMLEDHVDEEGQEFLDILQSSTANAVELTRTARDLATVLLQDDAETQRVSLERSLEQQVEAVRSAHSGAAVTIDGSVPDIHVAGTDMLEAVFRNLLSNAIQHNDKALPEVTVSVTEHDSEVEIRVADNGPGVPDAHKEAIFGKGERGLESEGTGIGLYLVKTLVESFGGDVCVEDNEPEGSVFVVRLPVAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUZ4</accession>
    <name>A0A1U7EUZ4_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF8009 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_1444A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUZ4"/>
    <dbReference type="EnsemblBacteria" id="CAI48813">
        <property type="protein sequence ID" value="CAI48813"/>
        <property type="gene ID" value="NP_1444A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_1444A"/>
    <dbReference type="eggNOG" id="arCOG04661">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_138335_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058322">
        <property type="entry name" value="DUF8009"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26033">
        <property type="entry name" value="DUF8009"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8009" evidence="2">
        <location>
            <begin position="26"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="175" mass="19754" checksum="B97CC7AD39157C26" modified="2017-05-10" version="1">MRQSDYVLPMTPNEGDATDGEPGKEDNTSIDDIETLVVDPDDVVQALAYNGQEEVGIDQKVSFELTPPFEAEAEPELVHIEDDTTKVDLEGAVRIRPFRFVEEGRQVVEQRPTRELALEELDAADPKEATIDEWIDEALETWKEHVREHRADVVDVYASHGMAIIDVEYTDSQES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV44</accession>
    <name>A0A1U7EV44_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Small CPxCG-related zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1556A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322503.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV44"/>
    <dbReference type="STRING" id="348780.NP_1556A"/>
    <dbReference type="EnsemblBacteria" id="CAI48869">
        <property type="protein sequence ID" value="CAI48869"/>
        <property type="gene ID" value="NP_1556A"/>
    </dbReference>
    <dbReference type="GeneID" id="41345079"/>
    <dbReference type="KEGG" id="nph:NP_1556A"/>
    <dbReference type="eggNOG" id="arCOG06412">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_206272_0_0_2"/>
    <dbReference type="OrthoDB" id="284396at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055982">
        <property type="entry name" value="DUF7560"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24441">
        <property type="entry name" value="DUF7560"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="50" mass="5265" checksum="2B48F228F397D3FE" modified="2017-05-10" version="1">MSEYIFSCPGCGQEIEVNGSMREAILSSGCPVCTTGVEKAAFESTSEATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV54</accession>
    <name>A0A1U7EV54_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Kef-type transport system</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">kef1</name>
        <name evidence="16" type="synonym">tp31:trkA4</name>
        <name evidence="16" type="ordered locus">NP_1576A</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a potassium transport system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV54"/>
    <dbReference type="STRING" id="348780.NP_1576A"/>
    <dbReference type="EnsemblBacteria" id="CAI48879">
        <property type="protein sequence ID" value="CAI48879"/>
        <property type="gene ID" value="NP_1576A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_1576A"/>
    <dbReference type="eggNOG" id="arCOG01957">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG01961">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005912_10_1_2"/>
    <dbReference type="OrthoDB" id="11709at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015079">
        <property type="term" value="F:potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="entry name" value="Regulator of K+ conductance, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006036">
        <property type="entry name" value="K_uptake_TrkA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32507">
        <property type="entry name" value="NA(+)/H(+) ANTIPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32507:SF0">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00335">
        <property type="entry name" value="KUPTAKETRKA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="TrkA C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="89"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="118"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="187"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="221"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="273"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="296"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="334"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="367"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="14">
        <location>
            <begin position="402"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="15">
        <location>
            <begin position="537"/>
            <end position="620"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAI48879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="621" mass="64117" checksum="1D1F1EAF41817DBA" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV56</accession>
    <name>A0A1U7EV56_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Halocyanin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">hcp5</name>
        <name evidence="5" type="ordered locus">NP_1600A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322525.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV56"/>
    <dbReference type="STRING" id="348780.NP_1600A"/>
    <dbReference type="EnsemblBacteria" id="CAI48891">
        <property type="protein sequence ID" value="CAI48891"/>
        <property type="gene ID" value="NP_1600A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701196"/>
    <dbReference type="KEGG" id="nph:NP_1600A"/>
    <dbReference type="eggNOG" id="arCOG02918">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084115_1_1_2"/>
    <dbReference type="OrthoDB" id="186995at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000923">
        <property type="entry name" value="BlueCu_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00127">
        <property type="entry name" value="Copper-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0186">Copper</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Blue (type 1) copper" evidence="4">
        <location>
            <begin position="70"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="23"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="98"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="37"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="98"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI48891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="208" mass="22421" checksum="CDF415F39DF1309B" modified="2017-05-10" version="1">MHTTPTRRTVVRTAAAAAGLAVAGCLDSEPDEAPEEAPEKAPDDRPTNAGELGTPTDEMTVLIHSQPWPEFEPQIIHVTPGTTVEWLVETGRHDVTAYHGDNHPPHRVPEETEPWASDHLPGPGATYERTFDTEGVYDYVDTQEVCISHEIAGNVGRVVVGWPDPDAEPALEPLSEAARADLPSQVVNALEMFNEETRPVLAAGPDGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV76</accession>
    <name>A0A1U7EV76_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transducer protein MpcT</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">mpcT</name>
        <name evidence="9" type="synonym">htr14</name>
        <name evidence="9" type="ordered locus">NP_1644A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <source>
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        <source>
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        <source>
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        </source>
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        </source>
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            <dbReference type="EMBL" id="CAI48913.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
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    <sequence length="607" mass="64238" checksum="F2AE7868682572BC" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV92</accession>
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        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
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            <end position="25"/>
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        <location>
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        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF24463"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI48938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
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                <person name="Oesterhelt D."/>
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            <dbReference type="PubMed" id="16169924"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
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        <location>
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            <end position="157"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="240" mass="26589" checksum="EB7F35F2EACD93BA" modified="2017-05-10" version="1">MEIREATADDGPAVRSVTLRSMEASYSLSPSTIESAIQQWYGVDNFAEKLNDDEVLLLVAEKDGEPVAFSESALVDDRGDIHWIHVAAMHRGEGIGQAMYEETRSQLEDAGAETIRGLVLSMNTEGNRFWENRGLQKAGEGTVEIDGTAFVENIYVDEDAVELQPVVVDGQELYIDRNDSSRGSSGPFYTVYTDEEGENHHSYYCGSCESLVTSMDTMGRLSCEECGNQLKPTRWDAAYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVC3</accession>
    <name>A0A1U7EVC3_NATPD</name>
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        <submittedName>
            <fullName evidence="2">UPF0339 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1732A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322590.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVC3"/>
    <dbReference type="EnsemblBacteria" id="CAI48957">
        <property type="protein sequence ID" value="CAI48957"/>
        <property type="gene ID" value="NP_1732A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701242"/>
    <dbReference type="KEGG" id="nph:NP_1732A"/>
    <dbReference type="eggNOG" id="arCOG06550">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_163886_1_0_2"/>
    <dbReference type="OrthoDB" id="108721at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010879">
        <property type="entry name" value="DUF1508"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051141">
        <property type="entry name" value="UPF0339_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036913">
        <property type="entry name" value="YegP-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041908">
        <property type="entry name" value="HVO_2922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40606">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40606:SF1">
        <property type="entry name" value="UPF0339 PROTEIN YEGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07411">
        <property type="entry name" value="DUF1508"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160113">
        <property type="entry name" value="YegP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1508" evidence="1">
        <location>
            <begin position="11"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="61" mass="6770" checksum="4219DD9BE2371B82" modified="2017-05-10" version="1">MSKATFEVYEDNAGQWRWRLVHDNGNIIADSGEGYASRQKCEQGIESVKENAPGADILKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVN1</accession>
    <name>A0A1U7EVN1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC-type transport system periplasmic substrate-binding protein (Probable substrate urea/short-chain amides)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">urtA</name>
        <name evidence="3" type="synonym">abc10s</name>
        <name evidence="3" type="ordered locus">NP_2004A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322722.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVN1"/>
    <dbReference type="STRING" id="348780.NP_2004A"/>
    <dbReference type="EnsemblBacteria" id="CAI49093">
        <property type="protein sequence ID" value="CAI49093"/>
        <property type="gene ID" value="NP_2004A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702401"/>
    <dbReference type="KEGG" id="nph:NP_2004A"/>
    <dbReference type="eggNOG" id="arCOG01022">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027128_1_1_2"/>
    <dbReference type="OrthoDB" id="200499at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028081">
        <property type="entry name" value="Leu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019968">
        <property type="entry name" value="Urea_ABC_transptr_substrate-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03669">
        <property type="entry name" value="urea_ABC_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47628">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47628:SF1">
        <property type="entry name" value="ALIPHATIC AMIDASE EXPRESSION-REGULATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13458">
        <property type="entry name" value="Peripla_BP_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Leucine-binding protein" evidence="2">
        <location>
            <begin position="37"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="423" mass="46942" checksum="46AE6D2A80EA57FA" modified="2017-05-10" version="1">MRNPTVSRRKLLASGAAAAGIGLAGCMGGNGGSDGPTVGILEDRSGNFQLNGTSKWQATRLAIEEINEDGGILGEEVEIVDPDPQSDNERYQELTERLILQDQVDALWAGYSSATREAIRPIINDEDQLYFYTTQYEGGVCDDTIFAVGSTARQQLGAVTPYLADQYGEDIYIIAADYNFGQLSGDWVNVIAEENGYNIVGEEYIPLDESDFSSVINRIQAEDPDFIMSMLVGANHENFYDQRDSNDLMIPIGTSTTMAQGHEHIRYEPNAVTDVYAGVSYMEELGDLREGENFVEDFYDRWPDANYLNQEAQNNYFSVYMWKEAVEEAGTFDQDAVIDVLEEGVEMNAPSGRIELDGATHHITHQMRVAHADENHDISFDAEQEIGPSFLREEVEGGDGCDLRAESKTTQYEPADVYGDILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVQ9</accession>
    <name>A0A1U7EVQ9_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF8159 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2092A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322765.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVQ9"/>
    <dbReference type="STRING" id="348780.NP_2092A"/>
    <dbReference type="EnsemblBacteria" id="CAI49137">
        <property type="protein sequence ID" value="CAI49137"/>
        <property type="gene ID" value="NP_2092A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702709"/>
    <dbReference type="KEGG" id="nph:NP_2092A"/>
    <dbReference type="eggNOG" id="arCOG06310">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1451449_0_0_2"/>
    <dbReference type="OrthoDB" id="290983at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058473">
        <property type="entry name" value="DUF8159"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26490">
        <property type="entry name" value="DUF8159"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8159" evidence="2">
        <location>
            <begin position="31"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="186" mass="20031" checksum="DFF4E153788D6BD5" modified="2017-05-10" version="1">MHRRTFVSGVAAGAVAGLAGCLGGDDDAPTTDEGHIEALRAEITDRGVEVDSVELEDRIVSVEHGFDEDPNDAIANVAMAFVERIIDEWDVERLEGHLHDEGNDWSWHAETEWAQEYADEEIGPEEYGQRISETMAMVIDEDGASETDTDEDDDAGDDGGENADDGSDGGDADGDADSDDEPTDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVY9</accession>
    <name>A0A1U7EVY9_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein eS19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 6" type="primary">rps19e</name>
        <name evidence="6" type="ordered locus">NP_2282A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May be involved in maturation of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322859.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVY9"/>
    <dbReference type="STRING" id="348780.NP_2282A"/>
    <dbReference type="EnsemblBacteria" id="CAI49232">
        <property type="protein sequence ID" value="CAI49232"/>
        <property type="gene ID" value="NP_2282A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703174"/>
    <dbReference type="KEGG" id="nph:NP_2282A"/>
    <dbReference type="eggNOG" id="arCOG01344">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108559_1_0_2"/>
    <dbReference type="OrthoDB" id="371836at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01474">
        <property type="entry name" value="Ribosomal_eS19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001266">
        <property type="entry name" value="Ribosomal_eS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027548">
        <property type="entry name" value="Ribosomal_eS19_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018277">
        <property type="entry name" value="Ribosomal_eS19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006811">
        <property type="entry name" value="PRK09333.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11710">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11710:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01090">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01413">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00628">
        <property type="entry name" value="RIBOSOMAL_S19E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI49232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="151" mass="16809" checksum="FFB4B7A186C2E10E" modified="2017-05-10" version="1">MTTLYDVPAEDLIEAVADELEDELEAPEWLAYSKTGTGRELPPEQDDFWSRRAASLLRKVAVDGPVGVERLRTAYGNSENGSTRYRVRPKHKQDGSGNVIRTALQQLEDAGYIETAEGRGRTITGDGRALLDETAEDVLESLDRPELERYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW31</accession>
    <name>A0A1U7EW31_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Homolog to phage PhiH1 repressor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2362A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322898.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW31"/>
    <dbReference type="STRING" id="348780.NP_2362A"/>
    <dbReference type="EnsemblBacteria" id="CAI49272">
        <property type="protein sequence ID" value="CAI49272"/>
        <property type="gene ID" value="NP_2362A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701422"/>
    <dbReference type="KEGG" id="nph:NP_2362A"/>
    <dbReference type="eggNOG" id="arCOG03923">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161782_1_0_2"/>
    <dbReference type="OrthoDB" id="285635at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="100" mass="11711" checksum="88A220B529D5DCAF" modified="2017-05-10" version="1">MTIRRNAGWQDRLDERILELLDEEPWSVPGMMTVEIPLNVTEQQIRERCLRMADIGLVSVEPDNGWMVQLTTEGRLYLEGDLDAELYPHPRHPKRLKESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW62</accession>
    <name>A0A1U7EW62_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type transport system permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">abc28p</name>
        <name evidence="7" type="ordered locus">NP_2440A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322937.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW62"/>
    <dbReference type="STRING" id="348780.NP_2440A"/>
    <dbReference type="DNASU" id="3702450"/>
    <dbReference type="EnsemblBacteria" id="CAI49311">
        <property type="protein sequence ID" value="CAI49311"/>
        <property type="gene ID" value="NP_2440A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702450"/>
    <dbReference type="KEGG" id="nph:NP_2440A"/>
    <dbReference type="eggNOG" id="arCOG01467">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1118251_0_0_2"/>
    <dbReference type="OrthoDB" id="203821at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047817">
        <property type="entry name" value="ABC2_TM_bact-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051784">
        <property type="entry name" value="Nod_factor_ABC_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43229:SF6">
        <property type="entry name" value="ABC-TYPE MULTIDRUG TRANSPORT SYSTEM, PERMEASE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43229">
        <property type="entry name" value="NODULATION PROTEIN J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006648">
        <property type="entry name" value="DrrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-2" evidence="6">
        <location>
            <begin position="23"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="259" mass="27349" checksum="C2E277EDDFC65639" modified="2017-05-10" version="1">MSRAGRIAAETRAAWYAFVRRRTAVFFTFFFPVILILIFGALVGTDPTGGGLFAEPAGYYVAGYLAVVVLFTPLSRVGSEVARHRSGNRFEKLATTPLSRAEWLFAQTLVNVVVIGLASLLILGLVLLVTDAAFTFSLLVVPYVALGVALFCGLGAILGRIADSQDGAIAASNGIAIPLLFLSETFVPPALLPEWFVPLLDLSPLTYFARGLRAATYEPVANTGGTGLVGSDPYLNLVVLAAGAVVFFAAGAYALPRTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW96</accession>
    <name>A0A1U7EW96_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Magnesium transporter MgtE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">tp32</name>
        <name evidence="11" type="ordered locus">NP_2472A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Acts as a magnesium transporter.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the SLC41A transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322953.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW96"/>
    <dbReference type="STRING" id="348780.NP_2472A"/>
    <dbReference type="EnsemblBacteria" id="CAI49327">
        <property type="protein sequence ID" value="CAI49327"/>
        <property type="gene ID" value="NP_2472A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703308"/>
    <dbReference type="KEGG" id="nph:NP_2472A"/>
    <dbReference type="eggNOG" id="arCOG00625">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037408_2_1_2"/>
    <dbReference type="OrthoDB" id="147899at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04606">
        <property type="entry name" value="CBS_pair_Mg_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.20">
        <property type="entry name" value="SLC41 divalent cation transporters, integral membrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006669">
        <property type="entry name" value="MgtE_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006667">
        <property type="entry name" value="SLC41_membr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036739">
        <property type="entry name" value="SLC41_membr_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00400">
        <property type="entry name" value="mgtE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41394">
        <property type="entry name" value="MAGNESIUM TRANSPORTER MGTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41394:SF5">
        <property type="entry name" value="SLC41A_MGTE INTEGRAL MEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01769">
        <property type="entry name" value="MgtE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161093">
        <property type="entry name" value="MgtE membrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0129">CBS domain</keyword>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="197"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="275"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="10">
        <location>
            <begin position="13"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="10">
        <location>
            <begin position="79"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI49327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="340" mass="36604" checksum="E1F40E75350BAAA9" modified="2017-05-10" version="1">MVETHADSARELVSNEYVSVDEDTFVGEAIREFREFSPVDEETTVYFLYVVDDQGNLTGVASFRELLNADEDEFVTDIMETDLVTIDADAHPEKVSATMSDTDFQALPVVDDGTLVGIVRADDMVDVVEEEATEDILKSAGMTFRDIEASRSTAILESSVPKILWLRLPWLIVALVGGLLAGAVIEEYEATLETAVALAFFIPVIMDMGGNVGTQASTIFVRGLALGHIDDRNAMRHFAREGLIGLAIGLIIGSLGAGAAYGWRVLRGDPHAETIAIVVFIALVAVCVVASVVGYVIPWLANKAGYDPAAVSDPLVTTIKDVTALLIYFGLATFLLAELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW97</accession>
    <name>A0A1U7EW97_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ArsR family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_2534A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322983.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW97"/>
    <dbReference type="STRING" id="348780.NP_2534A"/>
    <dbReference type="EnsemblBacteria" id="CAI49358">
        <property type="protein sequence ID" value="CAI49358"/>
        <property type="gene ID" value="NP_2534A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701478"/>
    <dbReference type="KEGG" id="nph:NP_2534A"/>
    <dbReference type="eggNOG" id="arCOG01685">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145184_0_0_2"/>
    <dbReference type="OrthoDB" id="11368at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051011">
        <property type="entry name" value="Metal_resp_trans_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43132">
        <property type="entry name" value="ARSENICAL RESISTANCE OPERON REPRESSOR ARSR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43132:SF2">
        <property type="entry name" value="ARSENICAL RESISTANCE OPERON REPRESSOR ARSR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12840">
        <property type="entry name" value="HTH_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="4">
        <location>
            <begin position="31"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="153" mass="16708" checksum="9C8E6836FD57215C" modified="2017-05-10" version="1">MSRLLPTRTDETPERSGDPAVLYVDDERAEQLFGALSAETTRDVFCVLNDAPCTASEVAERLGLSLQNAGYHLDKLREAELIEVVDVCYSEKGREMDVYAVAADPTVLVLGTEENRPRVRQAIERLVAGVGAPAALIAVWKSASDTVRELFGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWC3</accession>
    <name>A0A1U7EWC3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HesB/IscA family iron-sulfur cluster assembly accessory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2572A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323002.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWC3"/>
    <dbReference type="STRING" id="348780.NP_2572A"/>
    <dbReference type="EnsemblBacteria" id="CAI49377">
        <property type="protein sequence ID" value="CAI49377"/>
        <property type="gene ID" value="NP_2572A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701492"/>
    <dbReference type="KEGG" id="nph:NP_2572A"/>
    <dbReference type="eggNOG" id="arCOG04560">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069054_5_2_2"/>
    <dbReference type="OrthoDB" id="52656at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.300.12">
        <property type="entry name" value="HesB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016092">
        <property type="entry name" value="ATAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000361">
        <property type="entry name" value="ATAP_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017870">
        <property type="entry name" value="FeS_cluster_insertion_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035903">
        <property type="entry name" value="HesB-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00049">
        <property type="entry name" value="iron-sulfur cluster assembly accessory protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43011">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY 2 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43011:SF1">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY 2 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01521">
        <property type="entry name" value="Fe-S_biosyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89360">
        <property type="entry name" value="HesB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01152">
        <property type="entry name" value="HESB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core" evidence="1">
        <location>
            <begin position="10"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="117" mass="12597" checksum="5F35D6952CE91396" modified="2017-05-10" version="1">MSQSVDTDPVEVTEDAAEQAISLIENEGYDTDEAGLRLFVQQGGCAGLSYGLRFDDAPEDDDAITEHHGLRVFIDPSSQNYIEGSVLDFEGGLQGKGFSVENPNVESECGCGESFRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWD1</accession>
    <name>A0A1U7EWD1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF181 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2602A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323017.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWD1"/>
    <dbReference type="STRING" id="348780.NP_2602A"/>
    <dbReference type="EnsemblBacteria" id="CAI49392">
        <property type="protein sequence ID" value="CAI49392"/>
        <property type="gene ID" value="NP_2602A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701504"/>
    <dbReference type="KEGG" id="nph:NP_2602A"/>
    <dbReference type="eggNOG" id="arCOG02882">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020793_2_1_2"/>
    <dbReference type="OrthoDB" id="7433at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027624">
        <property type="entry name" value="TOMM_cyclo_SagD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003776">
        <property type="entry name" value="YcaO-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03604">
        <property type="entry name" value="TOMM_cyclo_SagD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37809">
        <property type="entry name" value="RIBOSOMAL PROTEIN S12 METHYLTHIOTRANSFERASE ACCESSORY FACTOR YCAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37809:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN S12 METHYLTHIOTRANSFERASE ACCESSORY FACTOR YCAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02624">
        <property type="entry name" value="YcaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51664">
        <property type="entry name" value="YCAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YcaO" evidence="1">
        <location>
            <begin position="233"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="562" mass="58308" checksum="4A387B9CDD48754C" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWF3</accession>
    <name>A0A1U7EWF3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glyoxalase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2650A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323041.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWF3"/>
    <dbReference type="STRING" id="348780.NP_2650A"/>
    <dbReference type="EnsemblBacteria" id="CAI49416">
        <property type="protein sequence ID" value="CAI49416"/>
        <property type="gene ID" value="NP_2650A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702759"/>
    <dbReference type="KEGG" id="nph:NP_2650A"/>
    <dbReference type="eggNOG" id="arCOG02708">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046006_8_4_2"/>
    <dbReference type="OrthoDB" id="358887at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004462">
        <property type="term" value="F:lactoylglutathione lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019243">
        <property type="term" value="P:methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06587">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46036">
        <property type="entry name" value="LACTOYLGLUTATHIONE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46036:SF5">
        <property type="entry name" value="LACTOYLGLUTATHIONE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="2"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="125" mass="13664" checksum="E14238AE1771D311" modified="2017-05-10" version="1">MAILHVCLNVADADRAVDWYTENLGFEESWGFETESGETVNRYVADDNGVELQLSDTEGETPSEDGDRWDHLAVSVDDVDAAVEAIDHHGIQQEPSDQPAAGARTAFIEDPDGHVIELIEPLNGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWF8</accession>
    <name>A0A1U7EWF8_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">acetyl-CoA C-acyltransferase</fullName>
            <ecNumber evidence="5">2.3.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">acaB2</name>
        <name evidence="8" type="ordered locus">NP_2612A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.16" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWF8"/>
    <dbReference type="STRING" id="348780.NP_2612A"/>
    <dbReference type="EnsemblBacteria" id="CAI49397">
        <property type="protein sequence ID" value="CAI49397"/>
        <property type="gene ID" value="NP_2612A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_2612A"/>
    <dbReference type="eggNOG" id="arCOG01282">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031026_1_1_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003988">
        <property type="term" value="F:acetyl-CoA C-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010124">
        <property type="term" value="P:phenylacetate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000010">
        <property type="entry name" value="Acetyl-CoA acetyltransferase (Thiolase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050215">
        <property type="entry name" value="Thiolase-like_sf_Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020610">
        <property type="entry name" value="Thiolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43853">
        <property type="entry name" value="3-KETOACYL-COA THIOLASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43853:SF2">
        <property type="entry name" value="3-OXOADIPYL-COA_3-OXO-5,6-DEHYDROSUBERYL-COA THIOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00099">
        <property type="entry name" value="THIOLASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="6">
        <location>
            <begin position="8"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="7">
        <location>
            <begin position="261"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="384" mass="40545" checksum="DCF9178719F37A04" modified="2017-05-10" version="1">MMSQRQPVIVQAVRTSQGTQGGVFAETGSEELSVPLVETMVEAAGLAPEDVDDVRWGCAKQVDEQSNNLARVIALLALGESVPGTTIDRLCASSAEAIMSAADAIRAGSRDVIVAGGVENMSRNERRTGIGSYEAIAERYDVDALAMGQTAEQVATEYDMSRERQDAYGARSQQRAVEATEEGRFDDEIVPIEGHDDEGNEVLVEEDEGLRPGTTAEAIADLPPAFEEGGTVTAANASQISDGAAGVLLTSRAFADERGLDILAAVEHHNVAGVDPTVMGIGPVPAVRGIWEETGRDADDYDLVELNEAFASQTLYCQDELGFDDEIFNVNGGAIAIGHPLGASGARLPVTLIHELQRRGGGRGLSTMCVGYGQGAAIELWVPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWH4</accession>
    <name>A0A1U7EWH4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UspA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2698A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323065.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWH4"/>
    <dbReference type="STRING" id="348780.NP_2698A"/>
    <dbReference type="EnsemblBacteria" id="CAI49440">
        <property type="protein sequence ID" value="CAI49440"/>
        <property type="gene ID" value="NP_2698A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701529"/>
    <dbReference type="KEGG" id="nph:NP_2698A"/>
    <dbReference type="eggNOG" id="arCOG02053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_11_4_2"/>
    <dbReference type="OrthoDB" id="105697at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd23659">
        <property type="entry name" value="USP_At3g01520-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF24">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="2"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="139" mass="15233" checksum="FF8D560C88D8EF05" modified="2017-05-10" version="1">MRVVVPYDGSEQAAEALDHAVSHHADDDVVLLRVLDFVDAGYDAPPEAALPGYWEDWYEQAESEAEAELEAAAADHDREFELETVVGRPARAIVEYVDENDIDHVVMGSHGRDGITRVLLGSVAEAVVRRSPVPVTVVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWK3</accession>
    <name>A0A1U7EWK3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">MaoC domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">maoC2</name>
        <name evidence="3" type="ordered locus">NP_2758A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWK3"/>
    <dbReference type="STRING" id="348780.NP_2758A"/>
    <dbReference type="EnsemblBacteria" id="CAI49470">
        <property type="protein sequence ID" value="CAI49470"/>
        <property type="gene ID" value="NP_2758A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_2758A"/>
    <dbReference type="eggNOG" id="arCOG00774">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094876_0_0_2"/>
    <dbReference type="OrthoDB" id="209979at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd03446">
        <property type="entry name" value="MaoC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002539">
        <property type="entry name" value="MaoC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052342">
        <property type="entry name" value="MCH/BMMD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43664:SF1">
        <property type="entry name" value="BETA-METHYLMALYL-COA DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43664">
        <property type="entry name" value="MONOAMINE OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01575">
        <property type="entry name" value="MaoC_dehydratas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MaoC-like" evidence="2">
        <location>
            <begin position="18"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="151" mass="16728" checksum="ED3DB73926BF9620" modified="2017-05-10" version="1">MTDEHQSVFEHTDIGDTDTTHGRTVTEADVTNFAGVSGDFNHLHTDEERMADSMFGERIAHGMFVLSAATGLLWQTRTPEEREAVVAFYGIDNLRFRQPVYIGDTIHVETEVVDTEAKPDGPGNGTVTTAIEVVNQRDQTVISCEMTSLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWK4</accession>
    <name>A0A1U7EWK4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2766A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323099.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWK4"/>
    <dbReference type="STRING" id="348780.NP_2766A"/>
    <dbReference type="EnsemblBacteria" id="CAI49474">
        <property type="protein sequence ID" value="CAI49474"/>
        <property type="gene ID" value="NP_2766A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701550"/>
    <dbReference type="KEGG" id="nph:NP_2766A"/>
    <dbReference type="eggNOG" id="arCOG03366">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092684_1_0_2"/>
    <dbReference type="OrthoDB" id="282430at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055946">
        <property type="entry name" value="DUF7524"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24368">
        <property type="entry name" value="DUF7524"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="189" mass="20102" checksum="948213D9B9AAC0E6" modified="2017-05-10" version="1">MTQTLAVDVNDDGLYTLSVPAEFEANGPFTVELHNHGEASHVYLNLDDRLSEVARIEATNHYLKENEQRRIDVATHDPSTWPDETLRGSLKVVVAHGQETRYVSVVLERAAETTTEVEVAPELSSPTVTEQSTEPTPLLRALPVVVAGSIAVLLAVGALFAAGGTQLLLGIGAVVAAAACGVAAYLLFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWM7</accession>
    <name>A0A1U7EWM7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transducer protein Htr25</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">htr25</name>
        <name evidence="8" type="ordered locus">NP_2760A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323096.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWM7"/>
    <dbReference type="STRING" id="348780.NP_2760A"/>
    <dbReference type="EnsemblBacteria" id="CAI49471">
        <property type="protein sequence ID" value="CAI49471"/>
        <property type="gene ID" value="NP_2760A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702840"/>
    <dbReference type="KEGG" id="nph:NP_2760A"/>
    <dbReference type="eggNOG" id="arCOG02318">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_116_1_2"/>
    <dbReference type="OrthoDB" id="8523at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11386">
        <property type="entry name" value="MCP_signal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.950">
        <property type="entry name" value="Methyl-accepting chemotaxis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32089:SF112">
        <property type="entry name" value="LYSOZYME-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32089">
        <property type="entry name" value="METHYL-ACCEPTING CHEMOTAXIS PROTEIN MCPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58104">
        <property type="entry name" value="Methyl-accepting chemotaxis protein (MCP) signaling domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="PAC" evidence="7">
        <location>
            <begin position="102"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="6">
        <location>
            <begin position="166"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="216"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="307"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="224"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="443" mass="48085" checksum="B2B04D0F317F13F6" modified="2017-05-10" version="1">MSDSTDGGEDPRPDGAGTLAERGNGIRWEQALNDMPLPIVVADFHGGDTELIIFNDLMEEMTGLTKAEAQEMDAWEIYRTDETHDTKQTMAERVRELGEPIRDHETELLDHDGNLHQVVISSTPIFDERGDVRATMTSFRDVTELRDKEEALQATQEQVAGTLDEAVSQLEAVAERVVDNAENIQQRASEQDDSLQDVAREMESLNASMEEVAASTDELAEAADEMRSAADEGRSAAEDAQEATDELLETGESLVDTVSALESEIGEISEVVDLIADIADQTNILALNANIEAARADAEGGGFKVVADEVRNLAEESQAHAADIREQIAVVEEKATETVAETETASERIEETGDRIQESLSALEGIAERIEESTSGIEEIADANTDQATRAEEVTATVDEVADRAQLVREAAGETVEAAETQRDVVGEVSAAVTQLRDRTDQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWX6</accession>
    <name>A0A1U7EWX6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3048A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323237.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWX6"/>
    <dbReference type="STRING" id="348780.NP_3048A"/>
    <dbReference type="EnsemblBacteria" id="CAI49615">
        <property type="protein sequence ID" value="CAI49615"/>
        <property type="gene ID" value="NP_3048A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701637"/>
    <dbReference type="KEGG" id="nph:NP_3048A"/>
    <dbReference type="HOGENOM" id="CLU_2420136_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="91" mass="10024" checksum="62A13B3B349BE467" modified="2017-05-10" version="1">MDPARVVTYGFVYAAAVFFLQWVLHPDPTAFEQGGLFSLLSALIFGFLGLYRRFGPETAERKPESYGLFVYGFVVLLVLLSGLTAASLVVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWY3</accession>
    <name>A0A1U7EWY3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3050A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323238.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWY3"/>
    <dbReference type="STRING" id="348780.NP_3050A"/>
    <dbReference type="EnsemblBacteria" id="CAI49616">
        <property type="protein sequence ID" value="CAI49616"/>
        <property type="gene ID" value="NP_3050A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701638"/>
    <dbReference type="KEGG" id="nph:NP_3050A"/>
    <dbReference type="eggNOG" id="arCOG04803">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_157002_0_0_2"/>
    <dbReference type="OrthoDB" id="241825at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055898">
        <property type="entry name" value="DUF7475"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24287">
        <property type="entry name" value="DUF7475"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="134" mass="14223" checksum="CB2A010C5A9A2FEF" modified="2017-05-10" version="1">MGATSTTSSASVFRKPGNPVGYIAIVMALVTGVLHLVAATNAIQFSQTLGILFVLNGLGFLGGIGLYLSKYWRKELFIVAALYSLVTILALFPVQGWGVEAFYMQGEINPLAVITKAAEAVLVVCALYLYATDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX23</accession>
    <name>A0A1U7EX23_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NP_1176A family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3126A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323276.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX23"/>
    <dbReference type="STRING" id="348780.NP_3126A"/>
    <dbReference type="EnsemblBacteria" id="CAI49654">
        <property type="protein sequence ID" value="CAI49654"/>
        <property type="gene ID" value="NP_3126A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701660"/>
    <dbReference type="KEGG" id="nph:NP_3126A"/>
    <dbReference type="eggNOG" id="arCOG00394">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_176006_1_0_2"/>
    <dbReference type="OrthoDB" id="350804at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="86" mass="9415" checksum="94C542905C44DA6C" modified="2017-05-10" version="1">MSDHDAAVEPLPPSAKLVYKTLEYEGPMSQSQLADASLLPQRTVRHALRKLDAAGVVEESVCLMDARKSTYELREPESSDPDTVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX41</accession>
    <name>A0A1U7EX41_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uridine phosphorylase</fullName>
            <ecNumber evidence="5">2.4.2.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">udp2</name>
        <name evidence="5" type="ordered locus">NP_3180A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PNP/UDP phosphorylase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.3" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323303.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX41"/>
    <dbReference type="STRING" id="348780.NP_3180A"/>
    <dbReference type="EnsemblBacteria" id="CAI49681">
        <property type="protein sequence ID" value="CAI49681"/>
        <property type="gene ID" value="NP_3180A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703441"/>
    <dbReference type="KEGG" id="nph:NP_3180A"/>
    <dbReference type="eggNOG" id="arCOG01324">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068457_0_1_2"/>
    <dbReference type="OrthoDB" id="372263at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004850">
        <property type="term" value="F:uridine phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009164">
        <property type="term" value="P:nucleoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17767">
        <property type="entry name" value="UP_EcUdp-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018016">
        <property type="entry name" value="Nucleoside_phosphorylase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43691">
        <property type="entry name" value="URIDINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43691:SF13">
        <property type="entry name" value="URIDINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01232">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside phosphorylase" evidence="4">
        <location>
            <begin position="16"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="241" mass="25323" checksum="6EAB62BDCD640CCC" modified="2017-05-10" version="1">MAKQPHLLVESGELADIALVPGDPGRVDRIADHCDDSEVVAENREYKVVNATYEGRELTICSTGIGSPSAAIAAEELAAVGVETFIRVGTTGALQSDIEIGDMVVATGAAKDEGTTKRYESTAVPAVPDYEVLDSLVDAAEARDAPVHVGPIATDDAFYAETETYVAEWEAAGLLCVEMEAAALFTLARRNGLSAGAICTVDGNLIEGTQKGETDDEELPEKAKDNVGRAIRIALEATTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX56</accession>
    <name>A0A1U7EX56_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Small CPxCG-related zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3192A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49687.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939577.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX56"/>
    <dbReference type="EnsemblBacteria" id="CAI49687">
        <property type="protein sequence ID" value="CAI49687"/>
        <property type="gene ID" value="NP_3192A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701681"/>
    <dbReference type="KEGG" id="nph:NP_3192A"/>
    <dbReference type="eggNOG" id="arCOG06371">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2821022_0_0_2"/>
    <dbReference type="OrthoDB" id="297362at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057157">
        <property type="entry name" value="DUF7835"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25205">
        <property type="entry name" value="DUF7835"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7835" evidence="2">
        <location>
            <begin position="6"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49687.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="67" mass="7665" checksum="0D3E463C83BA8417" modified="2017-05-10" version="1">MATRIDDDDAGMTEPCADCGRETPHDVSIELRTESDRAENAQFSREPYRVTSCRLCGRERSIRMNNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX64</accession>
    <name>A0A1U7EX64_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">CRISPR-associated protein Cas7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cas7</name>
        <name evidence="1" type="synonym">csc2</name>
        <name evidence="1" type="ordered locus">NP_3266A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323345.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX64"/>
    <dbReference type="STRING" id="348780.NP_3266A"/>
    <dbReference type="EnsemblBacteria" id="CAI49724">
        <property type="protein sequence ID" value="CAI49724"/>
        <property type="gene ID" value="NP_3266A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701708"/>
    <dbReference type="KEGG" id="nph:NP_3266A"/>
    <dbReference type="eggNOG" id="arCOG03482">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_843630_0_0_2"/>
    <dbReference type="OrthoDB" id="56442at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09709">
        <property type="entry name" value="Csc2_I-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017574">
        <property type="entry name" value="CRISPR-assoc_prot_Cas7/Csc2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03157">
        <property type="entry name" value="cas_Csc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18320">
        <property type="entry name" value="Csc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="329" mass="35342" checksum="07810345AE62330A" modified="2017-05-10" version="1">MIPDNITNALADGTVPIDGMTNTPGTNYTTLLVLRELDSHAIFTTNGQDADITSLSVVGDSGSIEYSPGLMFMRKQTGSDRRAGKAIQRELLEYDRSDSMDVNDMNPQSVESALYGSAASGDDDVDIGVTSRVMYDTAFTVRDASACIDEKFQNAPGEGYAKGSTANIREPDFFEPGTLFPCTITLRDATPAEVAFVTAITSRNKRYGAATTRLGRVNNRILGLYTGSEEGPSNRELTAETIVSFASESNRTLGDVVQAPALNADQAAAYVREAYDTACTETVAQTPVDDEVLDELLALTDDDDLRAVLEAQQPHSRSFIDDAIAADES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXA5</accession>
    <name>A0A1U7EXA5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable secreted glycoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3388A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323405.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXA5"/>
    <dbReference type="STRING" id="348780.NP_3388A"/>
    <dbReference type="EnsemblBacteria" id="CAI49785">
        <property type="protein sequence ID" value="CAI49785"/>
        <property type="gene ID" value="NP_3388A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701759"/>
    <dbReference type="KEGG" id="nph:NP_3388A"/>
    <dbReference type="eggNOG" id="arCOG08958">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG09394">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098910_0_0_2"/>
    <dbReference type="OrthoDB" id="214951at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="286" mass="29622" checksum="68D24E2947298421" modified="2017-05-10" version="1">MRRRTYIALSGAALASLAGCSDDEGENGDGAETPSDPEPTPGAEATDDNEETTPRNDVEETAEDEDPTEPEETPEQDDDTDRSDAGTLLSVTETLGGDQPLFDADGIERLDGEGAVVTDEMTLGSGLTVVVFEHTGAGNVIIELEGDRTELLVNDIGATSGAVAVPTPAGSYRFDVDAGGEWSLQVGQPRAPAEAVRTPPVSATGTGSDVVGPVELNGSETVAGEHDGSMNFIVEGYGEAASTSLAGELLFNELGTFDGETKTDLAGVVWLDIEADSDWSLDVTPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXA9</accession>
    <name>A0A1U7EXA9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Probable secreted glycoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">NP_3390A</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Cell wall</location>
            <location evidence="2">S-layer</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the halobacterial S-layer protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49786.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323406.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXA9"/>
    <dbReference type="EnsemblBacteria" id="CAI49786">
        <property type="protein sequence ID" value="CAI49786"/>
        <property type="gene ID" value="NP_3390A"/>
    </dbReference>
    <dbReference type="GeneID" id="69102741"/>
    <dbReference type="KEGG" id="nph:NP_3390A"/>
    <dbReference type="eggNOG" id="arCOG07560">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052936_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030115">
        <property type="term" value="C:S-layer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011635">
        <property type="entry name" value="CARDB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026371">
        <property type="entry name" value="PGF_CTERM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026452">
        <property type="entry name" value="Surf_glycop_sig_pep"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04207">
        <property type="entry name" value="halo_sig_pep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04126">
        <property type="entry name" value="PGF_CTERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07705">
        <property type="entry name" value="CARDB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0134">Cell wall</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0701">S-layer</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="8 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="436"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="CARDB" evidence="15">
        <location>
            <begin position="314"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="395"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="396"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAI49786.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="459" mass="48836" checksum="FD8DB8074F13D477" modified="2017-05-10" version="1">MYENNRILTGGWTRGQIRAVLLAAILVMSLVALAVPAAATGEDESATEHTSTADIGPTPAVSAVERGGADAALRSGGLFFQGQTVVLEVPESVTEQRDFQLRRYDRPGDRTASFVREFNVDGQDRYEISTNRLRGDYAVTLGNDQTQVVEFDSDGVATGVVDQADARPFEVAEQSLRVSWGQDRITTGDDDVDLNIRSNRARYNLNVSADGLDFDDLVNVFRHGGASDNPEPHADRNPFAVDGSVYDAYEDEDVLVVRGLRDGSLTADFTQIDAGDYPFRFEVTDTGVSSGAPASEETDPSPAFFDASDIEPMSATVAPGETVSVSATVTNTGETEGTQTVSLRIDDSPVANSTVGLEAGMSTTVDFEVDAPEEPGQYVHSITTDNATVAGSLTVEAPAETPTETPTETPTETPTETPTEETPETPTETPTEDQHGFGAVVAIVAALSAALLLARRQQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXC6</accession>
    <name>A0A1U7EXC6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tryptophan synthase beta chain</fullName>
            <ecNumber evidence="12">4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">trpB2</name>
        <name evidence="12" type="synonym">trpB</name>
        <name evidence="14" type="ordered locus">NP_3502A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 12">The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + L-tryptophan + H2O</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 12">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the TrpB family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="12"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323462.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXC6"/>
    <dbReference type="STRING" id="348780.NP_3502A"/>
    <dbReference type="EnsemblBacteria" id="CAI49842">
        <property type="protein sequence ID" value="CAI49842"/>
        <property type="gene ID" value="NP_3502A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703409"/>
    <dbReference type="KEGG" id="nph:NP_3502A"/>
    <dbReference type="eggNOG" id="arCOG01433">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016734_3_1_2"/>
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        <property type="reaction ID" value="UER00044"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06446">
        <property type="entry name" value="Trp-synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000004">
        <property type="entry name" value="Tryptophan synthase beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00133">
        <property type="entry name" value="Trp_synth_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006653">
        <property type="entry name" value="Trp_synth_b_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006654">
        <property type="entry name" value="Trp_synth_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023026">
        <property type="entry name" value="Trp_synth_beta/beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00263">
        <property type="entry name" value="trpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48077:SF3">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48077">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001413">
        <property type="entry name" value="Trp_syn_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00168">
        <property type="entry name" value="TRP_SYNTHASE_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 12" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="10 12 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 12" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="13">
        <location>
            <begin position="56"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="12">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAI49842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="389" mass="41554" checksum="968BCCDC70D6546D" modified="2017-05-10" version="1">MSDTDGMGDHEFGDFGGRYVPEPLQEPLEQLAGTFDDIVGDDGFQAEFRDLLGDFAGRPTPLYHARNLSDAYGADIYLKREDLLHGGAHKINNAVGQALLAKKAGKDRLIAETGAGQHGTATAMAGALFDLDTEIYMGRKDARRQQMNVFRMRLMGAEVNEVTRGEEGLAEAVDVALEDFAENIDTTHYLVGSVVGPDPFPRMVREFQSVIGKEAREQAIDRLGGLPDACVACVGGGSNAIGLFDAFRDDDVAFYGGEGGGQGSDSGSHAAPLAAGTEDVLHGMRTRVLEDDAEVHSVSAGLDYPGVGPEHAMFRAVGRCEYRAIEDDEALTAFRDLSELEGIIPALESAHAVALAGQVADETDHETIVVNLSGRGDKDMQQVAELLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXE8</accession>
    <name>A0A1U7EXE8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Probable transmembrane glycoprotein / HTH domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_3490A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323456.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXE8"/>
    <dbReference type="STRING" id="348780.NP_3490A"/>
    <dbReference type="EnsemblBacteria" id="CAI49836">
        <property type="protein sequence ID" value="CAI49836"/>
        <property type="gene ID" value="NP_3490A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701787"/>
    <dbReference type="KEGG" id="nph:NP_3490A"/>
    <dbReference type="eggNOG" id="arCOG00386">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1485937_0_0_2"/>
    <dbReference type="OrthoDB" id="116755at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055767">
        <property type="entry name" value="DUF7343"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24034">
        <property type="entry name" value="DUF7343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="HTH arsR-type" evidence="3">
        <location>
            <begin position="111"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="187" mass="19980" checksum="51A93BDA8FEE0A4A" modified="2017-05-10" version="1">MFPNALRDGRLVAAALLVVSTFALGVQLLNPPELVVAGGDDAALETVGYYFRYRDAIVVAVSAAVFGASAAFLALDAYPSDSEEKRSLETAVADSPEAGTSDGPGASESADETARRTRWEETAARLADTERIVYETALEADGEIAQREIVERTDLSKATVSRTLSNLESRGLLDRERRGMGNVVVLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXG7</accession>
    <name>A0A1U7EXG7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cupin 2 barrel domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3568A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323495.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXG7"/>
    <dbReference type="EnsemblBacteria" id="CAI49875">
        <property type="protein sequence ID" value="CAI49875"/>
        <property type="gene ID" value="NP_3568A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701810"/>
    <dbReference type="KEGG" id="nph:NP_3568A"/>
    <dbReference type="eggNOG" id="arCOG03008">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149791_1_0_2"/>
    <dbReference type="OrthoDB" id="190812at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin type-2" evidence="1">
        <location>
            <begin position="38"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="113" mass="12371" checksum="1877AC33843F0B95" modified="2017-05-10" version="1">MTHVSATDLLDELDDKQGNAVEVLDEDSLTVEIARHTAGEATPKNPHTEDELYYVVSGSGKIRVGDDVHSVESGDTVFVEQGLEHDFFDIGEDLVTLIVFADSSNPTSYSMRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXH3</accession>
    <name>A0A1U7EXH3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ribonuclease H domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3564A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXH3"/>
    <dbReference type="STRING" id="348780.NP_3564A"/>
    <dbReference type="EnsemblBacteria" id="CAI49873">
        <property type="protein sequence ID" value="CAI49873"/>
        <property type="gene ID" value="NP_3564A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3564A"/>
    <dbReference type="eggNOG" id="arCOG03131">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039905_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010995">
        <property type="entry name" value="DNA_repair_Rad51/TF_NusA_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038720">
        <property type="entry name" value="YprB_RNase_H-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14520">
        <property type="entry name" value="HHH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13482">
        <property type="entry name" value="RNase_H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47794">
        <property type="entry name" value="Rad51 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YprB ribonuclease H-like" evidence="2">
        <location>
            <begin position="308"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="490"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="502"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="514" mass="54679" checksum="338A551097B4FB49" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXJ6</accession>
    <name>A0A1U7EXJ6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Small CPxCG-related zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3634A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323527.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXJ6"/>
    <dbReference type="EnsemblBacteria" id="CAI49908">
        <property type="protein sequence ID" value="CAI49908"/>
        <property type="gene ID" value="NP_3634A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701831"/>
    <dbReference type="KEGG" id="nph:NP_3634A"/>
    <dbReference type="eggNOG" id="arCOG04534">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2461697_0_0_2"/>
    <dbReference type="OrthoDB" id="333406at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055993">
        <property type="entry name" value="DUF7571"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24455">
        <property type="entry name" value="DUF7571"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="89" mass="9724" checksum="2280538B9BE49382" modified="2017-05-10" version="1">MKPCQNCQTVIDEYILDKQLEPLRELTADDFSVCADCVTIVADACVECGGAVYVPRSESVTPDYCPACRSDLISRTGDDPGWTRCQASN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXL5</accession>
    <name>A0A1U7EXL5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cyclin domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3718A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323567.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXL5"/>
    <dbReference type="STRING" id="348780.NP_3718A"/>
    <dbReference type="EnsemblBacteria" id="CAI49950">
        <property type="protein sequence ID" value="CAI49950"/>
        <property type="gene ID" value="NP_3718A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703236"/>
    <dbReference type="KEGG" id="nph:NP_3718A"/>
    <dbReference type="eggNOG" id="arCOG04613">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155668_0_0_2"/>
    <dbReference type="OrthoDB" id="291244at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="102" mass="11240" checksum="846AD9D97BE83949" modified="2017-05-10" version="1">MYRARDHVENEQWLAAIDDAAERLDLGSEAKSRATDLFLSTVPEEDRSKQAVVAASLYAGALIAGDQRSQSDVADAVGVSRLTVQNRWRELLSEAGLEPPQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXL9</accession>
    <name>A0A1U7EXL9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Probable S-adenosylmethionine-dependent methyltransferase PrmC</fullName>
            <ecNumber evidence="6">2.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">prmC</name>
        <name evidence="6" type="ordered locus">NP_3674A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic/archaeal PrmC-related family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323546.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXL9"/>
    <dbReference type="STRING" id="348780.NP_3674A"/>
    <dbReference type="EnsemblBacteria" id="CAI49928">
        <property type="protein sequence ID" value="CAI49928"/>
        <property type="gene ID" value="NP_3674A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702809"/>
    <dbReference type="KEGG" id="nph:NP_3674A"/>
    <dbReference type="eggNOG" id="arCOG00109">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018398_6_2_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035657">
        <property type="term" value="C:eRF1 methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052190">
        <property type="entry name" value="Euk-Arch_PrmC-MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004557">
        <property type="entry name" value="PrmC-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00537">
        <property type="entry name" value="hemK_rel_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011527">
        <property type="entry name" value="PRK14968.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45875">
        <property type="entry name" value="METHYLTRANSFERASE N6AMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45875:SF1">
        <property type="entry name" value="METHYLTRANSFERASE N6AMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="5">
        <location>
            <begin position="36"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI49928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="191" mass="20757" checksum="8301FE0CE0214EBB" modified="2017-05-10" version="1">MTLEDRREMPTVYEPAEDSRLLADTAVERVDGGVALEVGVGFGYVAAQVASATETRVVGCDINPEACVRAREAGIETVRSNLTDPFAADSFDVVLFNPPYLPTPPEEEWDDPLEHALSGGEDGRRVIRPFLDDLGRVLRPAGRCLLLVSSLTDIDAVVKLAADAGFESREVAEESFPFERLVVLEITHANS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXP1</accession>
    <name>A0A1U7EXP1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GTPase HflX</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">GTP-binding protein HflX</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 10" type="primary">hflX</name>
        <name evidence="10" type="ordered locus">NP_3742A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">GTPase that associates with the 50S ribosomal subunit and may have a role during protein synthesis or ribosome biogenesis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer. Associates with the 50S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="5">May associate with membranes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. HflX GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323579.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXP1"/>
    <dbReference type="STRING" id="348780.NP_3742A"/>
    <dbReference type="EnsemblBacteria" id="CAI49962">
        <property type="protein sequence ID" value="CAI49962"/>
        <property type="gene ID" value="NP_3742A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702813"/>
    <dbReference type="KEGG" id="nph:NP_3742A"/>
    <dbReference type="eggNOG" id="arCOG00353">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019597_2_0_2"/>
    <dbReference type="OrthoDB" id="10150at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01878">
        <property type="entry name" value="HflX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2860">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11060">
        <property type="entry name" value="GTPase HflX, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00900">
        <property type="entry name" value="GTPase_HflX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030394">
        <property type="entry name" value="G_HFLX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032305">
        <property type="entry name" value="GTP-bd_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016496">
        <property type="entry name" value="GTPase_HflX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025121">
        <property type="entry name" value="GTPase_HflX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042108">
        <property type="entry name" value="GTPase_HflX_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03156">
        <property type="entry name" value="GTP_HflX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10229">
        <property type="entry name" value="GTP-BINDING PROTEIN HFLX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10229:SF8">
        <property type="entry name" value="GTPASE HFLX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16360">
        <property type="entry name" value="GTP-bdg_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13167">
        <property type="entry name" value="GTP-bdg_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006809">
        <property type="entry name" value="GTP-binding_hflX_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51705">
        <property type="entry name" value="G_HFLX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hflx-type G" evidence="9">
        <location>
            <begin position="191"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="107"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="197"/>
            <end position="204"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="258"/>
            <end position="261"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="324"/>
            <end position="327"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="352"/>
            <end position="354"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR006809-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR006809-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI49962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="435" mass="48597" checksum="56DA45B1220431D8" modified="2017-05-10" version="1">MTGTNGTAVVAKRVDDGHPDTAEIRDLAAAAGYTVAGEVTQTRTEDPALCLGEGKVAELADLVADTGATTVVFDNRLGPYQTYNLGNNLPKGTEVVDRFKLILDIFGQRAQTKKAQLQVELAELRYELPRAEAKASLAKRDERPGFMGLGEYDESREQDIKAQISRIRDELERIEQTEEHRREQRRESGFDLVALAGYTNAGKSTLLRRLAADVDIDENEGLHPDLDATAESEDRLFTTLGTTTRRADFDRRDVLVTDTVGFISDLPHWLVESFKSTLSEVYRADLVLLVVDVSDPVEEIREKLVTCHDTLYERNEAPIVTVLNKIDRVDDEELERKREALSALAPEPIVVSAKTGENVDSLRERIDEELPDWRRERLVVPMTDDTMSFVSWVHDHGHVDSVDYGDEVIIDFEARPAIIEQARARASELPAAAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXX0</accession>
    <name>A0A1U7EXX0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">mcm1</name>
        <name evidence="11" type="ordered locus">NP_3888A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323652.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXX0"/>
    <dbReference type="STRING" id="348780.NP_3888A"/>
    <dbReference type="EnsemblBacteria" id="CAI50035">
        <property type="protein sequence ID" value="CAI50035"/>
        <property type="gene ID" value="NP_3888A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701904"/>
    <dbReference type="KEGG" id="nph:NP_3888A"/>
    <dbReference type="eggNOG" id="arCOG00439">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000995_6_0_2"/>
    <dbReference type="OrthoDB" id="6747at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002469">
        <property type="entry name" value="Cell division control protein 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000003">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008047">
        <property type="entry name" value="MCM_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF66">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01660">
        <property type="entry name" value="MCMPROTEIN4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 11" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="10">
        <location>
            <begin position="272"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI50035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="676" mass="75627" checksum="DC7882CB1CC14244" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY08</accession>
    <name>A0A1U7EY08_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Phosphoribosylamine--glycine ligase</fullName>
            <ecNumber evidence="4 14">6.3.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">GARS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12 14">Glycinamide ribonucleotide synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13 14">Phosphoribosylglycinamide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14 17" type="primary">purD</name>
        <name evidence="17" type="ordered locus">NP_3972A</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>5-phospho-beta-D-ribosylamine + glycine + ATP = N(1)-(5-phospho-beta-D-ribosyl)glycinamide + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17453"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 14">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11 14">Belongs to the GARS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.13" evidence="4 14"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323693.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY08"/>
    <dbReference type="STRING" id="348780.NP_3972A"/>
    <dbReference type="EnsemblBacteria" id="CAI50077">
        <property type="protein sequence ID" value="CAI50077"/>
        <property type="gene ID" value="NP_3972A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703069"/>
    <dbReference type="KEGG" id="nph:NP_3972A"/>
    <dbReference type="eggNOG" id="arCOG04415">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027420_3_0_2"/>
    <dbReference type="OrthoDB" id="146558at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00125"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004637">
        <property type="term" value="F:phosphoribosylamine-glycine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.600.10">
        <property type="entry name" value="Phosphoribosylglycinamide synthetase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00138">
        <property type="entry name" value="GARS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020561">
        <property type="entry name" value="PRibGlycinamid_synth_ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000115">
        <property type="entry name" value="PRibGlycinamide_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020560">
        <property type="entry name" value="PRibGlycinamide_synth_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037123">
        <property type="entry name" value="PRibGlycinamide_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020559">
        <property type="entry name" value="PRibGlycinamide_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020562">
        <property type="entry name" value="PRibGlycinamide_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00877">
        <property type="entry name" value="purD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43472">
        <property type="entry name" value="PHOSPHORIBOSYLAMINE--GLYCINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43472:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLAMINE--GLYCINE LIGASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01071">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02843">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02844">
        <property type="entry name" value="GARS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01209">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01210">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00184">
        <property type="entry name" value="GARS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 14 17" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 14" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="16">
        <location>
            <begin position="111"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAI50077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="431" mass="46343" checksum="2CF2B59E72B299F6" modified="2017-05-10" version="1">MTETVLLVGGGGREHAIARAVDDSEHGTLYACASNRNPGIDELAAGYRELDETDAEAVVDYAESVDATLAAIGPESALNAGVADALDAAGVYAFGPREAEAKIETDKAFQREFMADENIPGCPDFATFEDPEAAADYIRDYDGDLAIKPRGLTGGKGVRVIGDQITAAEGVDYVLESDYEEFVLEERLVGEEFTVQAFVADGDYRVSPAVQDHKRAYEGDEGPNTGGMGSYTDATPELPFMDEADYEAAVDVIDAVVEAMPEYKGILYGQFMLTTDGPKVVEFNARFGDPEAMNTLPVLETDFVELLAAARDGEPLPELSFAEQATVCKYAVPDGYPTDPEAGARVEIDAESAGEAELFYASVDAREDGIYTTTSRSFAVVGIDDDISGAEQIVEAAFERTGEEGLRVRHDIGKPDLVQKRIDHMTELRGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY20</accession>
    <name>A0A1U7EY20_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3996A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323705.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY20"/>
    <dbReference type="STRING" id="348780.NP_3996A"/>
    <dbReference type="EnsemblBacteria" id="CAI50089">
        <property type="protein sequence ID" value="CAI50089"/>
        <property type="gene ID" value="NP_3996A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701933"/>
    <dbReference type="KEGG" id="nph:NP_3996A"/>
    <dbReference type="eggNOG" id="arCOG04606">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_194234_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055551">
        <property type="entry name" value="DUF7127"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23444">
        <property type="entry name" value="DUF7127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="78" mass="8538" checksum="7BCBC27CD5D39063" modified="2017-05-10" version="1">MREKLRTLRNDLDGPVRQYDYEDAAVLAADFGAVDGHVDVVDGTAIVVVDGEQYEFAVPADPARAVMNNGVVTIEMER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY73</accession>
    <name>A0A1U7EY73_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7508 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4182A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323798.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY73"/>
    <dbReference type="STRING" id="348780.NP_4182A"/>
    <dbReference type="EnsemblBacteria" id="CAI50182">
        <property type="protein sequence ID" value="CAI50182"/>
        <property type="gene ID" value="NP_4182A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701996"/>
    <dbReference type="KEGG" id="nph:NP_4182A"/>
    <dbReference type="eggNOG" id="arCOG08959">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2534767_0_0_2"/>
    <dbReference type="OrthoDB" id="103534at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055930">
        <property type="entry name" value="DUF7508"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24348">
        <property type="entry name" value="DUF7508"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7508" evidence="1">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="82" mass="9202" checksum="70F733C9E29B0F15" modified="2017-05-10" version="1">MPLQKPWQPLERSTIGGVPDRYGVYELGDGNGTVLAVEHGPLRDELKEALAYGDGSKVRWKETQTREQAVEIADTHRERLGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY92</accession>
    <name>A0A1U7EY92_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">AAA-type ATPase (CDC48 subfamily)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cdc48c</name>
        <name evidence="9" type="ordered locus">NP_4184A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family. CDC48 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323799.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY92"/>
    <dbReference type="STRING" id="348780.NP_4184A"/>
    <dbReference type="EnsemblBacteria" id="CAI50183">
        <property type="protein sequence ID" value="CAI50183"/>
        <property type="gene ID" value="NP_4184A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701997"/>
    <dbReference type="KEGG" id="nph:NP_4184A"/>
    <dbReference type="eggNOG" id="arCOG01308">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_12_2_2"/>
    <dbReference type="OrthoDB" id="77269at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000007">
        <property type="entry name" value="AAA family ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000178">
        <property type="entry name" value="CDC48/VCP homolog, AAA superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000018">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000012">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004201">
        <property type="entry name" value="Cdc48_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF171">
        <property type="entry name" value="NUCLEAR VALOSIN-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02933">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02359">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01072">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="Cdc48 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CDC48 N-terminal subdomain" evidence="8">
        <location>
            <begin position="6"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="CDC48" evidence="7">
        <location>
            <begin position="100"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="222"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="481"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="154"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI50183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="702" mass="75190" checksum="330E0131C13E96A2" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYF1</accession>
    <name>A0A1U7EYF1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Small CPxCG-related zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4328A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50255.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323871.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYF1"/>
    <dbReference type="STRING" id="348780.NP_4328A"/>
    <dbReference type="EnsemblBacteria" id="CAI50255">
        <property type="protein sequence ID" value="CAI50255"/>
        <property type="gene ID" value="NP_4328A"/>
    </dbReference>
    <dbReference type="GeneID" id="54763326"/>
    <dbReference type="KEGG" id="nph:NP_4328A"/>
    <dbReference type="eggNOG" id="arCOG04636">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2949230_0_0_2"/>
    <dbReference type="OrthoDB" id="179396at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057158">
        <property type="entry name" value="DUF7836"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25206">
        <property type="entry name" value="DUF7836"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7836" evidence="1">
        <location>
            <begin position="2"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50255.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="57" mass="6597" checksum="A78D562F3FAE5A5E" modified="2017-05-10" version="1">METYVRLLCPECGKNWQKSPSDLPEPSTTFHCPGCHASRRLSEFTRTEQDLKTLKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYQ9</accession>
    <name>A0A1U7EYQ9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ferredoxin (2Fe-2S) / UspA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ferA3</name>
        <name evidence="3" type="ordered locus">NP_4586A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323999.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYQ9"/>
    <dbReference type="STRING" id="348780.NP_4586A"/>
    <dbReference type="EnsemblBacteria" id="CAI50384">
        <property type="protein sequence ID" value="CAI50384"/>
        <property type="gene ID" value="NP_4586A"/>
    </dbReference>
    <dbReference type="GeneID" id="95969898"/>
    <dbReference type="KEGG" id="nph:NP_4586A"/>
    <dbReference type="eggNOG" id="arCOG00449">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02844">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_2_1_2"/>
    <dbReference type="OrthoDB" id="105697at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053441">
        <property type="entry name" value="2Fe2S_Ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041393">
        <property type="entry name" value="Frdxn_Halo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF6">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN UP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="2">
        <location>
            <begin position="290"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="390" mass="41473" checksum="556B0A8B254CE8FB" modified="2017-05-10" version="1">MYDTILVPTDGSDVAERAGTVGVRMAALFDADLTLLSVQEPGDGDRAERAHEALKEAAAEADISPQTKTIDGGETSVHRSIIDYAGAHDVDIIVMGTHGRTGIGRFLLGSVAEQTLKESPIPVVTVHEDTARDFAVDNVLVPTDGSEGAKAATEHAVDLAAEVGATLHTLQVTSDGDKAEPSPVTDVRNRGETAGIEVVTATRSGRPHEAIAGYVAEAGIDVVVMGAHGRGGIRQYLLGSVTERTVRFSPVPVISVKPETDGVTVEFLDYEAVDDNGWSLEDDDLFEKAAEADLPAGAYGTIATEKGEYILDAAERDGHDWPFFCRGGGCINCAAVLVDGEVEMETNRSLSDEEVDEMDLRLTCVATPATDEVKLVYNAKQLDQLRDRIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYY0</accession>
    <name>A0A1U7EYY0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4706A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324058.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYY0"/>
    <dbReference type="STRING" id="348780.NP_4706A"/>
    <dbReference type="EnsemblBacteria" id="CAI50444">
        <property type="protein sequence ID" value="CAI50444"/>
        <property type="gene ID" value="NP_4706A"/>
    </dbReference>
    <dbReference type="GeneID" id="69102746"/>
    <dbReference type="KEGG" id="nph:NP_4706A"/>
    <dbReference type="eggNOG" id="arCOG02859">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114391_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058927">
        <property type="entry name" value="OB_2TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26045">
        <property type="entry name" value="OB_2TM_halo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="172" mass="18124" checksum="8B04003053FB2E3B" modified="2017-05-10" version="1">MHRRVEAALLVGVCLLAVGLCVAYGATEPAPDRHDYPEVDDLGVSPDHPIESPASIGGTVAATDPVVITASVDGNTVAYVVRDGPAVTVGQELWVFGPVEAVDGPVDTGAGTDATVVAQVDAERAVVQDPWEVGYMYAVSALAVLWVLFRAVRHWRLDTTARAVVPRGERRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ21</accession>
    <name>A0A1U7EZ21_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">IS200-type transposase ISNph5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4812A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324109.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ21"/>
    <dbReference type="STRING" id="348780.NP_4812A"/>
    <dbReference type="EnsemblBacteria" id="CAI50497">
        <property type="protein sequence ID" value="CAI50497"/>
        <property type="gene ID" value="NP_4812A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702185"/>
    <dbReference type="KEGG" id="nph:NP_4812A"/>
    <dbReference type="eggNOG" id="arCOG02759">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101320_2_2_2"/>
    <dbReference type="OrthoDB" id="92826at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1290">
        <property type="entry name" value="Transposase IS200-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002686">
        <property type="entry name" value="Transposase_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036515">
        <property type="entry name" value="Transposase_17_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033573">
        <property type="entry name" value="transpos_IS200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33360">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33360:SF4">
        <property type="entry name" value="TRANSPOSASE IS200-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01797">
        <property type="entry name" value="Y1_Tnp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01321">
        <property type="entry name" value="Y1_Tnp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143422">
        <property type="entry name" value="Transposase IS200-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS200-like" evidence="1">
        <location>
            <begin position="12"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="130" mass="14998" checksum="E3A1F971EF7152DC" modified="2017-05-10" version="1">MEYHLQSGSHTVYALQYHFVTVTKYRADILTDERLERVAEVAHEIAEDFEADIKNVDGGTDHVHILFTTKPTTDLTKFINSLKGVTSRRIRQEYPEVKQTLEDAFWQPGYFLATTGQVSIDVLMDYVENQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ49</accession>
    <name>A0A1U7EZ49_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">TrkA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">trkA1</name>
        <name evidence="9" type="synonym">tp29u1</name>
        <name evidence="9" type="ordered locus">NP_4902A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a potassium transport system.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324154.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ49"/>
    <dbReference type="STRING" id="348780.NP_4902A"/>
    <dbReference type="EnsemblBacteria" id="CAI50542">
        <property type="protein sequence ID" value="CAI50542"/>
        <property type="gene ID" value="NP_4902A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703304"/>
    <dbReference type="KEGG" id="nph:NP_4902A"/>
    <dbReference type="eggNOG" id="arCOG01959">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046525_0_0_2"/>
    <dbReference type="OrthoDB" id="27588at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015079">
        <property type="term" value="F:potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="entry name" value="Regulator of K+ conductance, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006036">
        <property type="entry name" value="K_uptake_TrkA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050721">
        <property type="entry name" value="Trk_Ktr_HKT_K-transport"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007031">
        <property type="entry name" value="PRK09496.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007034">
        <property type="entry name" value="PRK09496.2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007039">
        <property type="entry name" value="PRK09496.3-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43833">
        <property type="entry name" value="POTASSIUM CHANNEL PROTEIN 2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43833:SF5">
        <property type="entry name" value="TRK SYSTEM POTASSIUM UPTAKE PROTEIN TRKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00335">
        <property type="entry name" value="KUPTAKETRKA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="TrkA C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0630">Potassium</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RCK N-terminal" evidence="7">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="8">
        <location>
            <begin position="139"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="7">
        <location>
            <begin position="227"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="8">
        <location>
            <begin position="364"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI50542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="445" mass="47389" checksum="452D05DDB9B636C1" modified="2017-05-10" version="1">MRIVIVGAGEVGTSIADSLAADHDVVVVDIDEDRAETLKYDIDVMTLAGDGTALSTLQTADVGSADLFIASTDDDRANLVACGTAKTIGDPFTIARTNSVEYLRTWELTESAFGVDFMVCSDLLTAEDIVRVVGLPSAVDVDPFAGGLVQMAEFEICAGSPVAGQTVAEADRFESLTFAGLFRDGEMVLPQGDTVVKPGDRAVVIGSPESVQAFASDLAPEATPGGADEIVIIGGSEIGYQTARLLEERGLKPRLIEQDHDRARKLAEELPETLVMEHDATDTEFLAREHVDEADIVVAALDSDERNLLVSILASRLGTERIVAVVDSAEYVDLFEEIGIDVAVNPRQVTAEEITRFTHGGVAENIAVLENDQAEVLELELTDSGSLTGQTVQEVADGLDAELVFGAITRGRTLIAPRGDTELLPGDHIVVFVETAFVDELTRMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ62</accession>
    <name>A0A1U7EZ62_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Small CPxCG-related zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4952A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324179.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ62"/>
    <dbReference type="STRING" id="348780.NP_4952A"/>
    <dbReference type="EnsemblBacteria" id="CAI50567">
        <property type="protein sequence ID" value="CAI50567"/>
        <property type="gene ID" value="NP_4952A"/>
    </dbReference>
    <dbReference type="GeneID" id="54763330"/>
    <dbReference type="KEGG" id="nph:NP_4952A"/>
    <dbReference type="eggNOG" id="arCOG09270">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_213599_0_0_2"/>
    <dbReference type="OrthoDB" id="209680at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="48" mass="5106" checksum="25F7E7DDF42DE581" modified="2017-05-10" version="1">MSVSTTHRCRSCGFEAPPDADAWGNVQHPPLGELTQCPECGSTDTTTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ95</accession>
    <name>A0A1U7EZ95_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF188 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5082A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324242.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ95"/>
    <dbReference type="STRING" id="348780.NP_5082A"/>
    <dbReference type="EnsemblBacteria" id="CAI50632">
        <property type="protein sequence ID" value="CAI50632"/>
        <property type="gene ID" value="NP_5082A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702248"/>
    <dbReference type="KEGG" id="nph:NP_5082A"/>
    <dbReference type="eggNOG" id="arCOG04312">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107892_2_0_2"/>
    <dbReference type="OrthoDB" id="15280at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09879">
        <property type="entry name" value="PIN_VapC_AF0591-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041120">
        <property type="entry name" value="PIN_9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18477">
        <property type="entry name" value="PIN_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VapC9 PIN-like" evidence="1">
        <location>
            <begin position="3"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="124" mass="13331" checksum="AF6C8934833B5E6D" modified="2017-05-10" version="1">MRVVLDTNALMAPVEVGVRTFAELDRLLGEYEAVVPQAVVEELESLSGGGGEEATAASVGADLAERECAVVEHNAEYADDAVVELGREADYAVTSDMPLRERLLEANVPVISLRGRTKFEITEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZA4</accession>
    <name>A0A1U7EZA4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_5104A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324253.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZA4"/>
    <dbReference type="STRING" id="348780.NP_5104A"/>
    <dbReference type="EnsemblBacteria" id="CAI50643">
        <property type="protein sequence ID" value="CAI50643"/>
        <property type="gene ID" value="NP_5104A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702256"/>
    <dbReference type="KEGG" id="nph:NP_5104A"/>
    <dbReference type="eggNOG" id="arCOG04730">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_188093_0_0_2"/>
    <dbReference type="OrthoDB" id="226203at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057176">
        <property type="entry name" value="DUF7854"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25252">
        <property type="entry name" value="DUF7854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="86" mass="9560" checksum="504DEB9973A7B847" modified="2017-05-10" version="1">MDRIAALRQIESALSAFEAGEADLETCERRIRAAVRTFATEFDGERSAYRADTGTVVVAASEREARERVRELTDADAPTVERIGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZD1</accession>
    <name>A0A1U7EZD1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_5102A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324252.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZD1"/>
    <dbReference type="STRING" id="348780.NP_5102A"/>
    <dbReference type="EnsemblBacteria" id="CAI50642">
        <property type="protein sequence ID" value="CAI50642"/>
        <property type="gene ID" value="NP_5102A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702255"/>
    <dbReference type="KEGG" id="nph:NP_5102A"/>
    <dbReference type="eggNOG" id="arCOG04801">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2140264_0_0_2"/>
    <dbReference type="OrthoDB" id="269514at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058577">
        <property type="entry name" value="DUF7855_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057177">
        <property type="entry name" value="DUF7855_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25253">
        <property type="entry name" value="DUF7855"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26293">
        <property type="entry name" value="DUF7855_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7855" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7855" evidence="2">
        <location>
            <begin position="66"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="112" mass="12773" checksum="70AB4949C334B96E" modified="2017-05-10" version="1">MLLVVTYSTAARTSLRNVCRTHEESVIRRFGRAALLADTHHGAFLALRLREKHPDDVQLERTEPFNEFRDLPASVREAAEAYENRTEPATAYAKFAVDSPHPSPDELKRQEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZJ7</accession>
    <name>A0A1U7EZJ7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5320A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324360.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZJ7"/>
    <dbReference type="EnsemblBacteria" id="CAI50751">
        <property type="protein sequence ID" value="CAI50751"/>
        <property type="gene ID" value="NP_5320A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702331"/>
    <dbReference type="KEGG" id="nph:NP_5320A"/>
    <dbReference type="eggNOG" id="arCOG04683">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1269885_0_0_2"/>
    <dbReference type="OrthoDB" id="338759at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055521">
        <property type="entry name" value="DUF7095"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23378">
        <property type="entry name" value="DUF7095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="218" mass="23627" checksum="07CD63BED89AFB48" modified="2017-05-10" version="1">MSLSRDAAVERLRSVVETVAEDPTPVPVREVWVFGDLVLGLDPVDYLGVYLTKDLLFKDAPEREAEFESELGVSGVGKTVSAAWAEANPDCVRANASGHAAPEQCLAAYLFGDEPLHLEVCNTGFEDNVTQRLRGAQARGDYTQLLDPRAVCLWVDTDDGGTTSEEAFEKLAAGEFVFPTLAESLSMLGLDDPEAETAAEQLQKHRQEADSLVRGDVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZR0</accession>
    <name>A0A1U7EZR0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2065H</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CCI69577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZR0"/>
    <dbReference type="STRING" id="348780.NP_2065H"/>
    <dbReference type="EnsemblBacteria" id="CCI69577">
        <property type="protein sequence ID" value="CCI69577"/>
        <property type="gene ID" value="NP_2065H"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_2065H"/>
    <dbReference type="eggNOG" id="arCOG14548">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3148066_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCI69577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="48" mass="5208" checksum="2B574F50542BB5CC" modified="2017-05-10" version="1">MTVLYALFLVTKPADITYDAWVHALVFVPPLVGFAGHALRLALRPGDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILV7</accession>
    <name>Q3ILV7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_6266A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILV7"/>
    <dbReference type="EnsemblBacteria" id="CAI50913">
        <property type="protein sequence ID" value="CAI50913"/>
        <property type="gene ID" value="NP_6266A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_6266A"/>
    <dbReference type="HOGENOM" id="CLU_996059_0_0_2"/>
    <dbReference type="OrthoDB" id="326212at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="281" mass="30610" checksum="F86ACA23A9140909" modified="2005-11-08" version="1">MKACPETATLASVDDLSGHLWVQELPTGGTFRFQVAASGLVTFAVGDRTFDSAESVPIQYRRAAQLITNQIDRAALQAATSASSTVTFCGMTTWNEGLNYEWDVLPAFVGVDVWSSSKETFLSPDAATGVFKRLGLPTLPAIEKEASAAHTDFARFDNDTAFPESAWRSGKAAGVLLRDKSDGHVTVWQSECDESPSATRGPSVTELADTYATNERIERTVELLEDDTATPTVASVRDRLIADIAREAYVELFTDGEFIVSFPEFRSVVAQRVQQHQSLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILZ5</accession>
    <name>Q3ILZ5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Pyridoxal phosphate-dependent aminotransferase</fullName>
            <ecNumber evidence="6">2.6.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">NP_6188A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6 7">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILZ5"/>
    <dbReference type="EnsemblBacteria" id="CAI50874">
        <property type="protein sequence ID" value="CAI50874"/>
        <property type="gene ID" value="NP_6188A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_6188A"/>
    <dbReference type="HOGENOM" id="CLU_016922_10_0_2"/>
    <dbReference type="OrthoDB" id="6534at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000004">
        <property type="entry name" value="Acetylornithine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050103">
        <property type="entry name" value="Class-III_PLP-dep_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986:SF79">
        <property type="entry name" value="ACETYLORNITHINE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986">
        <property type="entry name" value="AMINOTRANSFERASE CLASS III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4 5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI50874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="440" mass="47407" checksum="E96D2EE953D78736" modified="2005-11-08" version="1">MSQEHSPQTTNSEIEDQYEQYLMPIWKDLDVPIRRAEGCTVEDFDGNEYLDVFSGIAVTNAGHRNDAVVEAAKDQLDEFIHGCSYLHPHQPAAELAKRLAEITPGDLEKSFFANSGTEAVEGAIKLARKYTGSKEVIALEMSFHGRTLGSLALTGNKGYKNEMAPTINDVAHVAPPYAYRCQLCDGGPCSNDCGDRLEQVIQTHTAGDLAAVVVEPVMGEGGIIVPPEGWLERVQEITHDHGGLLIVDEVQAGYGRTGEMWASDHFDVVPDIMPQAKGIANGLPLGAFTARPEIADAFESGDHLSTFGGNPVACAAALETIEQLEAGLIDNARTQGEWLTSRLEELEADHEVIGDTRGLGLMQGIELIDAGGETGPMDVAPEPDAKLAKKVSHHLREEGIVIGVGGFHGNVLRFQPPLSISRDQLERTVDAIDDALSAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM29</accession>
    <name>Q3IM29_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Trk-type transport system (Probable substrate potassium)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">trkH3</name>
        <name evidence="10" type="synonym">tp30u2</name>
        <name evidence="10" type="ordered locus">NP_6112A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10 11">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TrkH potassium transport family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324438.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM29"/>
    <dbReference type="EnsemblBacteria" id="CAI50836">
        <property type="protein sequence ID" value="CAI50836"/>
        <property type="gene ID" value="NP_6112A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694607"/>
    <dbReference type="KEGG" id="nph:NP_6112A"/>
    <dbReference type="HOGENOM" id="CLU_030708_3_0_2"/>
    <dbReference type="OrthoDB" id="111943at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
        <property type="term" value="P:metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003445">
        <property type="entry name" value="Cat_transpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32024">
        <property type="entry name" value="TRK SYSTEM POTASSIUM UPTAKE PROTEIN TRKG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32024:SF2">
        <property type="entry name" value="TRK SYSTEM POTASSIUM UPTAKE PROTEIN TRKG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02386">
        <property type="entry name" value="TrkH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="159"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="209"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="285"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="320"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="389"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="458"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="519"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI50836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="551" mass="58588" checksum="0BA4ADC3BC8A02B4" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM78</accession>
    <name>Q3IM78_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ISH6-type transposase ISNph3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_6002A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324389.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM78"/>
    <dbReference type="EnsemblBacteria" id="CAI50781">
        <property type="protein sequence ID" value="CAI50781"/>
        <property type="gene ID" value="NP_6002A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694575"/>
    <dbReference type="KEGG" id="nph:NP_6002A"/>
    <dbReference type="HOGENOM" id="CLU_613401_0_0_2"/>
    <dbReference type="OrthoDB" id="198848at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041731">
        <property type="entry name" value="transpos_ISH6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="446" mass="50378" checksum="D4AA03740D67BC51" modified="2005-11-08" version="1">MHATIDVRLTVSIDDHKTIPLATLAESITDQNIESVLLEGLVESLDAARVEALCGEKHTHGNGENRFQRAGTDTRTAVTTAGEHELDLHYVKDTAADHDESSYFRPVEDVLGFDGQNRYQQDIAAKSVDLATSLSYRDAADHGDGIFERMPSPTTINRRTKEYGTKLKQFLPDCVADTDADAVIPDGTQCHSQDEDRSYHSVQATLGENTAEESRSLLDLSVNADWDETAAELEDIGAVTDDATVVSDGDSGIVTAFTDEHSDHQLDLVHVGRTLDYNLWDDGVFSLDRRNQIVSEVIEDVFHLKNSVIKHRPEEEFAAIRERITRTTERIEKTAWQLNQYGSEKAARYLRRWLPSIVRFAKQAIEGFEVPWTSNPVERLMGEVSKRCKNQWMRWTKEGLEAILQLRLVKYADPSYYQWFLDELLQRPTKTAMSCDLSIESTRGKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMI0</accession>
    <name>THI4_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thiamine thiazole synthase</fullName>
            <ecNumber evidence="1">2.4.2.60</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thi4</name>
        <name type="ordered locus">NP_5174A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[ADP-thiazole synthase]-L-cysteine + glycine + NAD(+) = [ADP-thiazole synthase]-dehydroalanine + ADP-5-ethyl-4-methylthiazole-2-carboxylate + nicotinamide + 3 H2O + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:55708"/>
            <dbReference type="Rhea" id="RHEA-COMP:14264"/>
            <dbReference type="Rhea" id="RHEA-COMP:14265"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:90873"/>
            <dbReference type="ChEBI" id="CHEBI:139151"/>
            <dbReference type="EC" id="2.4.2.60"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homooctamer; tetramer of dimers.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">During the catalytic reaction, a sulfide is transferred from Cys-166 to a reaction intermediate, generating a dehydroalanine residue.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the THI4 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.60" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324288.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMI0"/>
    <dbReference type="SMR" id="Q3IMI0"/>
    <dbReference type="STRING" id="348780.NP_5174A"/>
    <dbReference type="EnsemblBacteria" id="CAI50678">
        <property type="protein sequence ID" value="CAI50678"/>
        <property type="gene ID" value="NP_5174A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703243"/>
    <dbReference type="KEGG" id="nph:NP_5174A"/>
    <dbReference type="eggNOG" id="arCOG00574">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053727_2_0_2"/>
    <dbReference type="OrthoDB" id="4240at2157"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160205">
        <property type="term" value="F:cysteine-dependent adenosine diphosphate thiazole synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052837">
        <property type="term" value="P:thiazole biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00304">
        <property type="entry name" value="Thi4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002922">
        <property type="entry name" value="Thi4_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022828">
        <property type="entry name" value="Thi4_prok"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00292">
        <property type="entry name" value="sulfide-dependent adenosine diphosphate thiazole synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43422">
        <property type="entry name" value="THIAMINE THIAZOLE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43422:SF3">
        <property type="entry name" value="THIAMINE THIAZOLE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01946">
        <property type="entry name" value="Thi4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000259382" description="Thiamine thiazole synthase">
        <location>
            <begin position="1"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="203"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="62"/>
            <end position="63"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>glycine</name>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="2,3-didehydroalanine (Cys)" evidence="1">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="309" mass="32910" checksum="1D96EC83C65DEB4F" modified="2005-11-08" version="1">MTEFDQFSDVGEAEVTRAIGQEWTEEFMDFSDSDVIIVGGGPSGLMAAKELSERGVQTMVVEKNNYLGGGFWLGGFLMNKVTVRDPAQNVLEDLDVDFKRSQDSEGLYVANGPEACSGLIKAACDAGAKMQNMTEFTDIVIREDHRVGGIVMNWTPVHALPREITCVDPIAVEADLVIDATGHDAVAVSKLQERGVLNAPGIEHADEHNTGMDQTGDDSYGAPGHDSPGHDSMWVGQSEDAVVEHTGLAHEGLIVTGMATATTYGLPRMGPTFGAMLLSGKRAAQAALDELGVDAPDVEMTTRAEPADD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMY1</accession>
    <name>RL29_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL29</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L29</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl29</name>
        <name type="ordered locus">NP_4868A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL29 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324137.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMY1"/>
    <dbReference type="SMR" id="Q3IMY1"/>
    <dbReference type="STRING" id="348780.NP_4868A"/>
    <dbReference type="EnsemblBacteria" id="CAI50525">
        <property type="protein sequence ID" value="CAI50525"/>
        <property type="gene ID" value="NP_4868A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703136"/>
    <dbReference type="KEGG" id="nph:NP_4868A"/>
    <dbReference type="eggNOG" id="arCOG00785">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158491_2_2_2"/>
    <dbReference type="OrthoDB" id="11736at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.310:FF:000001">
        <property type="entry name" value="50S ribosomal protein L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00374">
        <property type="entry name" value="Ribosomal_uL29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001854">
        <property type="entry name" value="Ribosomal_uL29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018254">
        <property type="entry name" value="Ribosomal_uL29_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036049">
        <property type="entry name" value="Ribosomal_uL29_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00012">
        <property type="entry name" value="L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00831">
        <property type="entry name" value="Ribosomal_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46561">
        <property type="entry name" value="Ribosomal protein L29 (L29p)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00579">
        <property type="entry name" value="RIBOSOMAL_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_1000007538" description="Large ribosomal subunit protein uL29">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="69" mass="7717" checksum="1925E837C81BEEFC" modified="2005-11-08" version="1">MAILHVDEIRDMTAAEREVELEQLETELLNEKAVLAAGGAPENPGRIGELKRTIARVKTIQREEGDFDE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q3INE8</accession>
    <name>PDXS_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyridoxal 5'-phosphate synthase subunit PdxS</fullName>
            <shortName evidence="1">PLP synthase subunit PdxS</shortName>
            <ecNumber evidence="1">4.3.3.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Pdx1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pdxS</name>
        <name type="ordered locus">NP_4528A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of pyridoxal 5'-phosphate from ribose 5-phosphate (RBP), glyceraldehyde 3-phosphate (G3P) and ammonia. The ammonia is provided by the PdxT subunit. Can also use ribulose 5-phosphate and dihydroxyacetone phosphate as substrates, resulting from enzyme-catalyzed isomerization of RBP and G3P, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + phosphate + 3 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31507"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="4.3.3.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; pyridoxal 5'-phosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">In the presence of PdxT, forms a dodecamer of heterodimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PdxS/SNZ family.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.6" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323970.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3INE8"/>
    <dbReference type="SMR" id="Q3INE8"/>
    <dbReference type="STRING" id="348780.NP_4528A"/>
    <dbReference type="EnsemblBacteria" id="CAI50355">
        <property type="protein sequence ID" value="CAI50355"/>
        <property type="gene ID" value="NP_4528A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703089"/>
    <dbReference type="KEGG" id="nph:NP_4528A"/>
    <dbReference type="eggNOG" id="arCOG04075">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055352_1_0_2"/>
    <dbReference type="OrthoDB" id="6840at2157"/>
    <dbReference type="UniPathway" id="UPA00245"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036381">
        <property type="term" value="F:pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008615">
        <property type="term" value="P:pyridoxine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04727">
        <property type="entry name" value="pdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000001">
        <property type="entry name" value="Pyridoxine biosynthesis protein PDX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01824">
        <property type="entry name" value="PdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001852">
        <property type="entry name" value="PdxS/SNZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033755">
        <property type="entry name" value="PdxS/SNZ_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003215">
        <property type="entry name" value="PRK04180.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31829">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNZ1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31829:SF0">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNZ1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01680">
        <property type="entry name" value="SOR_SNZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029271">
        <property type="entry name" value="Pdx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01235">
        <property type="entry name" value="PDXS_SNZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51129">
        <property type="entry name" value="PDXS_SNZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0704">Schiff base</keyword>
    <feature type="chain" id="PRO_1000070392" description="Pyridoxal 5'-phosphate synthase subunit PdxS">
        <location>
            <begin position="1"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="273"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="292"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with D-ribose 5-phosphate" evidence="1">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="242"/>
            <end position="243"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="301" mass="32449" checksum="948A1598CDAFA602" modified="2005-11-08" version="1">MSNTDLEELRRGTDLVKRGFAKMQKGGVIMDVVTREQARIAEDAGAVAVMHLESVPADIRKRGGVARMADPGGLEDVIDEVSIPVMGKARIGHTAEAQILEATGADMIDESEVLTQADDRYHIDKREFTAPFVCGARNLGEALRRIDEGAAMIRTKGEAGTGDVNQAVTHQRNIQRSIRKLSGMDYEERDEWAREHGAPRELVHETAEMGRLPVVNFAAGGIATPADAALMMQLGCDGIFVGSGIFGAENPQAMGEAVVAAVNNYDDPETLKEIAKSPGKGMKGQANETLPEEEKLQDRGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IPH6</accession>
    <name>Q3IPH6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase</fullName>
            <shortName evidence="1">HPPK</shortName>
            <ecNumber evidence="1">2.7.6.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">6-hydroxymethyl-7,8-dihydropterin diphosphokinase</fullName>
            <shortName evidence="1">6-HMPDK</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">7,8-dihydro-6-hydroxymethylpterin diphosphokinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">7,8-dihydro-6-hydroxymethylpterin pyrophosphokinase</fullName>
            <shortName evidence="1">PPPK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1 3" type="primary">mptE</name>
        <name evidence="3" type="ordered locus">NP_3768A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of diphosphate from ATP to 6-hydroxymethyl-7,8-dihydropterin (6-HMD), leading to 6-hydroxymethyl-7,8-dihydropterin diphosphate (6-HMDP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>6-hydroxymethyl-7,8-dihydropterin + ATP = (7,8-dihydropterin-6-yl)methyl diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11412"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:44841"/>
            <dbReference type="ChEBI" id="CHEBI:72950"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate: step 4/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal 6-HMPDK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.3" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323592.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IPH6"/>
    <dbReference type="STRING" id="348780.NP_3768A"/>
    <dbReference type="EnsemblBacteria" id="CAI49975">
        <property type="protein sequence ID" value="CAI49975"/>
        <property type="gene ID" value="NP_3768A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701860"/>
    <dbReference type="KEGG" id="nph:NP_3768A"/>
    <dbReference type="eggNOG" id="arCOG04303">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093043_0_0_2"/>
    <dbReference type="OrthoDB" id="34207at2157"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00155"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003848">
        <property type="term" value="F:2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02131">
        <property type="entry name" value="HMPDK_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027510">
        <property type="entry name" value="HMPDK_MptE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002826">
        <property type="entry name" value="MptE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39648">
        <property type="entry name" value="6-HYDROXYMETHYL-7,8-DIHYDROPTERIN PYROPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39648:SF1">
        <property type="entry name" value="6-HYDROXYMETHYL-7,8-DIHYDROPTERIN PYROPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01973">
        <property type="entry name" value="MptE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="6-hydroxymethylpterin diphosphokinase MptE-like" evidence="2">
        <location>
            <begin position="50"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="229" mass="24090" checksum="F210405034A55056" modified="2005-11-08" version="1">MLHREWQPAYEAILTAFGYDAAGDVAARDVLFGLVTDAAPLKSDSIDLPGTVAVCGGGPSLAADLDRARRADTVVAASTAADVCRDAGVAVDCMVTDLDKNPETAVELAAAGTPVAVHAHGDNVDLIEAYVPAIDVGSVLPTTQAAPRPPVVNPGGFTDGDRAAFLADACGADRLVFPGWDFDDTAVSAAKAEKLRWAARLLRWLELRRNEEFGVLDGRRGDIRLPWLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IPX6</accession>
    <name>Q3IPX6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ThiJ/PfpI domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_3462A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase C56 family. HSP31-like subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323442.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IPX6"/>
    <dbReference type="STRING" id="348780.NP_3462A"/>
    <dbReference type="EnsemblBacteria" id="CAI49822">
        <property type="protein sequence ID" value="CAI49822"/>
        <property type="gene ID" value="NP_3462A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702796"/>
    <dbReference type="KEGG" id="nph:NP_3462A"/>
    <dbReference type="eggNOG" id="arCOG00769">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070319_0_2_2"/>
    <dbReference type="OrthoDB" id="8461at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019172">
        <property type="term" value="F:glyoxalase III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019243">
        <property type="term" value="P:methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03141">
        <property type="entry name" value="GATase1_Hsp31_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002818">
        <property type="entry name" value="DJ-1/PfpI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050325">
        <property type="entry name" value="Prot/Nucl_acid_deglycase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48094:SF11">
        <property type="entry name" value="GLUTATHIONE-INDEPENDENT GLYOXALASE HSP31-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48094">
        <property type="entry name" value="PROTEIN/NUCLEIC ACID DEGLYCASE DJ-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01965">
        <property type="entry name" value="DJ-1_PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="DJ-1/PfpI" evidence="4">
        <location>
            <begin position="6"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="226" mass="23826" checksum="2417831A1E1C9020" modified="2005-11-08" version="1">MTRTLFVVSEDGYWAEECIEPLTTLEDAGVETTVATPTGAPPVVDEASLDPEEIGAEAAEELRAIDEDHPKLNDPVPVAGEDADDYDAVVFPGGHGTCWDINQDADARRLLVDAVSGDDGTALVVCHAVGLLAFARTDDGKPLVAGRSVTGFPNAWEADLVDDHDRLPDGRKLPYWVEDEVKAVGGEWDAELDAETSVTVDGDLITARGPGSSAAAAETLLSALDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IQ02</accession>
    <name>Q3IQ02_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Smc-like protein Sph1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">sph1</name>
        <name evidence="5" type="ordered locus">NP_3410A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the Sph1/Sph2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323416.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IQ02"/>
    <dbReference type="STRING" id="348780.NP_3410A"/>
    <dbReference type="EnsemblBacteria" id="CAI49796">
        <property type="protein sequence ID" value="CAI49796"/>
        <property type="gene ID" value="NP_3410A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701769"/>
    <dbReference type="KEGG" id="nph:NP_3410A"/>
    <dbReference type="eggNOG" id="arCOG00373">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_434529_0_0_2"/>
    <dbReference type="OrthoDB" id="241568at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045487">
        <property type="entry name" value="ASRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32114">
        <property type="entry name" value="ABC TRANSPORTER ABCH.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32114:SF2">
        <property type="entry name" value="ABC TRANSPORTER ABCH.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="177"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="315"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="177"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00049666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="591" mass="67064" checksum="94EC99319CF0B7C4" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-01-23" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IQI3</accession>
    <name>PSTB2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphate import ATP-binding protein PstB 2</fullName>
            <ecNumber evidence="1">7.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ABC phosphate transporter 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphate-transporting ATPase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pstB2</name>
        <name type="synonym">abc22a</name>
        <name type="ordered locus">NP_3044A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the ABC transporter complex PstSACB involved in phosphate import. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>phosphate(out) + ATP + H2O = ADP + 2 phosphate(in) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24440"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.3.2.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The complex is composed of two ATP-binding proteins (PstB), two transmembrane proteins (PstC and PstA) and a solute-binding protein (PstS).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. Phosphate importer (TC 3.A.1.7) family.</text>
    </comment>
    <dbReference type="EC" id="7.3.2.1" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323235.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IQI3"/>
    <dbReference type="SMR" id="Q3IQI3"/>
    <dbReference type="STRING" id="348780.NP_3044A"/>
    <dbReference type="EnsemblBacteria" id="CAI49613">
        <property type="protein sequence ID" value="CAI49613"/>
        <property type="gene ID" value="NP_3044A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702432"/>
    <dbReference type="KEGG" id="nph:NP_3044A"/>
    <dbReference type="eggNOG" id="arCOG00923">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="OrthoDB" id="57213at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015415">
        <property type="term" value="F:ATPase-coupled phosphate ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03260">
        <property type="entry name" value="ABC_PstB_phosphate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005670">
        <property type="entry name" value="PstB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43423">
        <property type="entry name" value="ABC TRANSPORTER I FAMILY MEMBER 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43423:SF1">
        <property type="entry name" value="ABC TRANSPORTER I FAMILY MEMBER 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51238">
        <property type="entry name" value="PSTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0592">Phosphate transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000272592" description="Phosphate import ATP-binding protein PstB 2">
        <location>
            <begin position="1"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="1">
        <location>
            <begin position="36"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="68"/>
            <end position="75"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="284" mass="30827" checksum="6CB9525693563FEA" modified="2005-11-08" version="1">MTLLSTLRGISSPARQQPGTQSESRRGGDTLAERGLAVAGVSHGFDGTAVLESVTLAVDRGETVAIIGPSGTGKTTLLRLLALFSEPDDGTIGLDGTDVWRQSERQRLAARRRIGMVFQKANLFDTTVRRNVRYGLSVRRSWRERLRSWLGGRKRQESVFEALDIVGLADAASQSASSLSGGEAQRVAFARALAYDPDFLLLDEPTSDLDPRNTAVIEEAVDAARSRGLGVAVATHDMHQARRIADRIVVLLDGQVIEAGPTERVFSDPDDARARKFINGELVY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IQT0</accession>
    <name>ENO_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Enolase</fullName>
            <ecNumber evidence="1">4.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">2-phospho-D-glycerate hydro-lyase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">2-phosphoglycerate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">eno</name>
        <name type="ordered locus">NP_2846A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible conversion of 2-phosphoglycerate (2-PG) into phosphoenolpyruvate (PEP). It is essential for the degradation of carbohydrates via glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2-phosphoglycerate = phosphoenolpyruvate + H2O</text>
            <dbReference type="Rhea" id="RHEA:10164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.2.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds a second Mg(2+) ion via substrate during catalysis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell surface</location>
        </subcellularLocation>
        <text evidence="1">Fractions of enolase are present in both the cytoplasm and on the cell surface.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the enolase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323139.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IQT0"/>
    <dbReference type="SMR" id="Q3IQT0"/>
    <dbReference type="STRING" id="348780.NP_2846A"/>
    <dbReference type="EnsemblBacteria" id="CAI49514">
        <property type="protein sequence ID" value="CAI49514"/>
        <property type="gene ID" value="NP_2846A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702674"/>
    <dbReference type="KEGG" id="nph:NP_2846A"/>
    <dbReference type="eggNOG" id="arCOG01169">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031223_0_1_2"/>
    <dbReference type="OrthoDB" id="8680at2157"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00187"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00318">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020809">
        <property type="entry name" value="Enolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01060">
        <property type="entry name" value="eno"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902:SF1">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001400">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00002">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00178">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00164">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0964">Secreted</keyword>
    <feature type="chain" id="PRO_0000267147" description="Enolase">
        <location>
            <begin position="1"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="325"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>(2R)-2-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>(2R)-2-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="354"/>
        </location>
        <ligand>
            <name>(2R)-2-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="355"/>
        </location>
        <ligand>
            <name>(2R)-2-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="376"/>
        </location>
        <ligand>
            <name>(2R)-2-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00318"/>
        </source>
    </evidence>
    <sequence length="398" mass="42112" checksum="9274F41A64FB9B25" modified="2005-11-08" version="1">MTLITEVRLRKILDSRGNPTVEAEVRTENGGFGRGAAPSGASTGEYEAVERPAEEAIAQARELAVPRIEGQVYAGDQRGVDNALHGADGTDDFSEIGANSAVAISMAAAKAAADVLGAPLYQHLGGAFRGRDFPVPLGNVIGGGEHAADATHIQEFLAAPVGAPSVADAVFANAKVHGEVQRQLDERGVPAAKGDEGAWAPSIDDSEAFEIMEEAIAAVEDEVGFEIRFGLDVAGAELYEDGVYHYGDETRSPDEQVAYIADLVEEYNLVYVEDPLDEDDFSGFAELTERVGDRTLICGDDLFVTNTERLQRGIDDGAANSILIKPNQIGTLSDAFDAIELATRNGYDPVVSHRSGETEDTTIAHLAVATDAPFIKTGAVGGERTAKLNELIRIEANA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IQT6</accession>
    <name>RPO3_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo3</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo3</name>
        <name evidence="1" type="synonym">rpoD</name>
        <name type="ordered locus">NP_2834A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal Rpo3/eukaryotic RPB3 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323133.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IQT6"/>
    <dbReference type="SMR" id="Q3IQT6"/>
    <dbReference type="STRING" id="348780.NP_2834A"/>
    <dbReference type="EnsemblBacteria" id="CAI49508">
        <property type="protein sequence ID" value="CAI49508"/>
        <property type="gene ID" value="NP_2834A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703154"/>
    <dbReference type="KEGG" id="nph:NP_2834A"/>
    <dbReference type="eggNOG" id="arCOG04241">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038421_3_1_2"/>
    <dbReference type="OrthoDB" id="84933at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.12">
        <property type="entry name" value="DNA-directed RNA polymerase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00320">
        <property type="entry name" value="RNApol_arch_Rpo3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011262">
        <property type="entry name" value="DNA-dir_RNA_pol_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011263">
        <property type="entry name" value="DNA-dir_RNA_pol_RpoA/D/Rpb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022842">
        <property type="entry name" value="RNAP_Rpo3/Rpb3/RPAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036643">
        <property type="entry name" value="RNApol_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050518">
        <property type="entry name" value="Rpo3/RPB3_RNA_Pol_subunit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001988">
        <property type="entry name" value="PRK00783.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800:SF2">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01000">
        <property type="entry name" value="RNA_pol_A_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01193">
        <property type="entry name" value="RNA_pol_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00662">
        <property type="entry name" value="RPOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56553">
        <property type="entry name" value="Insert subdomain of RNA polymerase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000005789" description="DNA-directed RNA polymerase subunit Rpo3">
        <location>
            <begin position="1"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00320"/>
        </source>
    </evidence>
    <sequence length="247" mass="27118" checksum="94FBCC5810CD3DAF" modified="2005-11-08" version="1">MSEEYEVTFIDRDERSARFLVRGVTPAFANGLRRAMIADVPTLSIDTLRVVENSSVMFDEQLALRLGLVPLTTPDDYEPGETVTLAIDVEGPATAYSGDLVSSDDKVQPADENIPIIELKDDQQLELEADATLGRGKDHAKHQGGVAVGYRHLQRVEVVGEKGEFEDDEPEILRGVIEEDGDLIETGEFDNDLSQRYPGKEVEVEEVPNAFVFDVETDGSMPVEELVLKAAESIAARADELEEAVAL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-07-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IQZ0</accession>
    <name>MEND_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase</fullName>
            <shortName evidence="1">SEPHCHC synthase</shortName>
            <ecNumber evidence="1">2.2.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Menaquinone biosynthesis protein MenD</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">menD</name>
        <name type="ordered locus">NP_2726A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>isochorismate + 2-oxoglutarate + H(+) = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2</text>
            <dbReference type="Rhea" id="RHEA:25593"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29780"/>
            <dbReference type="ChEBI" id="CHEBI:58818"/>
            <dbReference type="EC" id="2.2.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
        <text evidence="1">Binds 1 thiamine pyrophosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Quinol/quinone metabolism; 1,4-dihydroxy-2-naphthoate biosynthesis; 1,4-dihydroxy-2-naphthoate from chorismate: step 2/7.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Quinol/quinone metabolism; menaquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TPP enzyme family. MenD subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.9" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323079.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IQZ0"/>
    <dbReference type="SMR" id="Q3IQZ0"/>
    <dbReference type="STRING" id="348780.NP_2726A"/>
    <dbReference type="EnsemblBacteria" id="CAI49454">
        <property type="protein sequence ID" value="CAI49454"/>
        <property type="gene ID" value="NP_2726A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702910"/>
    <dbReference type="KEGG" id="nph:NP_2726A"/>
    <dbReference type="eggNOG" id="arCOG04611">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006051_3_0_2"/>
    <dbReference type="OrthoDB" id="212847at2157"/>
    <dbReference type="UniPathway" id="UPA00079"/>
    <dbReference type="UniPathway" id="UPA01057">
        <property type="reaction ID" value="UER00164"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070204">
        <property type="term" value="F:2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009234">
        <property type="term" value="P:menaquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07037">
        <property type="entry name" value="TPP_PYR_MenD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02009">
        <property type="entry name" value="TPP_SHCHC_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01659">
        <property type="entry name" value="MenD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004433">
        <property type="entry name" value="MenaQ_synth_MenD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00173">
        <property type="entry name" value="menD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42916">
        <property type="entry name" value="2-SUCCINYL-5-ENOLPYRUVYL-6-HYDROXY-3-CYCLOHEXENE-1-CARBOXYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42916:SF1">
        <property type="entry name" value="PROTEIN PHYLLO, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004983">
        <property type="entry name" value="MenD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0474">Menaquinone biosynthesis</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000341900" description="2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase">
        <location>
            <begin position="1"/>
            <end position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01659"/>
        </source>
    </evidence>
    <sequence length="586" mass="61363" checksum="CCB24E0249D80A6E" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IS55</accession>
    <name>DPHB_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Diphthine synthase</fullName>
            <ecNumber evidence="1">2.1.1.98</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Diphthamide biosynthesis methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dphB</name>
        <name type="ordered locus">NP_1884A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the trimethylation of the amino group of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine. The three successive methylation reactions represent the second step of diphthamide biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + 3 S-adenosyl-L-methionine = diphthine-[translation elongation factor 2] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:36415"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="Rhea" id="RHEA-COMP:10172"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="ChEBI" id="CHEBI:82696"/>
            <dbReference type="EC" id="2.1.1.98"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the diphthine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.98" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322665.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IS55"/>
    <dbReference type="SMR" id="Q3IS55"/>
    <dbReference type="STRING" id="348780.NP_1884A"/>
    <dbReference type="EnsemblBacteria" id="CAI49033">
        <property type="protein sequence ID" value="CAI49033"/>
        <property type="gene ID" value="NP_1884A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701290"/>
    <dbReference type="KEGG" id="nph:NP_1884A"/>
    <dbReference type="eggNOG" id="arCOG04161">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066040_0_0_2"/>
    <dbReference type="OrthoDB" id="39139at2157"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004164">
        <property type="term" value="F:diphthine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11647">
        <property type="entry name" value="DHP5_DphB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01084">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004551">
        <property type="entry name" value="Dphthn_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00522">
        <property type="entry name" value="dph5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882:SF0">
        <property type="entry name" value="DIPHTHINE METHYL ESTER SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882">
        <property type="entry name" value="DIPHTHINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036432">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000064827" description="Diphthine synthase">
        <location>
            <begin position="1"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="113"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01084"/>
        </source>
    </evidence>
    <sequence length="266" mass="28033" checksum="A82F4D8D86FF516C" modified="2005-11-08" version="1">MLTFVGLGLYDERSITVAGREALRSADRVFAEFYTSKLAGATVEELQNHHDIDIEVRDRAGVEQEPGPILDAAEAGGAVFLTAGDTMISTTHVDLRLRADNRGIETQIIHGTTAGAAAASLSGLQNYRFGKATTLPFPYAHGGEGVPGSVLDTIADNRKRGLHTLVYLDIKVGTGPRGPDPDHEEYMTADYAAELLAEDLDTVGVAIARAGSPDPVLAADRLSALADRSFGDPLHLLIVPGDLHVVEHDALVALAGAPESALPDPV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-09-05" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ISI8</accession>
    <name>RL44E_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Large ribosomal subunit protein eL42</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>50S ribosomal protein L44e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">rpl44e</name>
        <name type="synonym">rpl36aR</name>
        <name type="ordered locus">NP_1614A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="3">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic ribosomal protein eL42 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322532.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ISI8"/>
    <dbReference type="SMR" id="Q3ISI8"/>
    <dbReference type="STRING" id="348780.NP_1614A"/>
    <dbReference type="EnsemblBacteria" id="CAI48898">
        <property type="protein sequence ID" value="CAI48898"/>
        <property type="gene ID" value="NP_1614A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703141"/>
    <dbReference type="KEGG" id="nph:NP_1614A"/>
    <dbReference type="eggNOG" id="arCOG04109">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114645_3_0_2"/>
    <dbReference type="OrthoDB" id="52456at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01476">
        <property type="entry name" value="Ribosomal_L44e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000552">
        <property type="entry name" value="Ribosomal_eL44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053708">
        <property type="entry name" value="Ribosomal_LSU_eL42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004425">
        <property type="entry name" value="PRK05767.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00935">
        <property type="entry name" value="Ribosomal_L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01172">
        <property type="entry name" value="RIBOSOMAL_L44E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000419127" description="Large ribosomal subunit protein eL42">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="2">
        <location>
            <begin position="11"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="93" mass="10748" checksum="E076E0C0B0EE5706" modified="2005-11-08" version="1">MEMPRRFNTYCPHCHSHFEHEVEKVRSGRSSGTKWDARRTRRGKAVIGNAGRFSKVPGGDKPTKKTDLKYRCSECGKAHLREGWRAGRLTFQE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ISW0</accession>
    <name>RS6E_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein eS6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">30S ribosomal protein S6e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps6e</name>
        <name type="ordered locus">NP_1368A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322410.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ISW0"/>
    <dbReference type="SMR" id="Q3ISW0"/>
    <dbReference type="STRING" id="348780.NP_1368A"/>
    <dbReference type="EnsemblBacteria" id="CAI48775">
        <property type="protein sequence ID" value="CAI48775"/>
        <property type="gene ID" value="NP_1368A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703185"/>
    <dbReference type="KEGG" id="nph:NP_1368A"/>
    <dbReference type="eggNOG" id="arCOG01946">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109671_1_1_2"/>
    <dbReference type="OrthoDB" id="7793at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00512">
        <property type="entry name" value="Ribosomal_eS6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001377">
        <property type="entry name" value="Ribosomal_eS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020924">
        <property type="entry name" value="Ribosomal_eS6_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018282">
        <property type="entry name" value="Ribosomal_eS6_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003294">
        <property type="entry name" value="PRK04290.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11502">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01092">
        <property type="entry name" value="Ribosomal_S6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01405">
        <property type="entry name" value="Ribosomal_S6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00578">
        <property type="entry name" value="RIBOSOMAL_S6E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000258626" description="Small ribosomal subunit protein eS6">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="106"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="129" mass="13463" checksum="8CAC1302C977DED3" modified="2005-11-08" version="1">MADFQVVVGDDDGTAYSFEVDGQDANRFIGRAIGETVDGDAVGLPGYELEITGGSDQSGRPMHGDINGAETAAILSEGGVGFNPTVDGERKRVTVRGAEVSEDTRQINASIVSRGEQSIDDLLGGEDDE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-05-20" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IUB7</accession>
    <name>DNAJ_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Chaperone protein DnaJ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dnaJ</name>
        <name type="ordered locus">NP_0346A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) ions per monomer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DnaJ family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321902.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IUB7"/>
    <dbReference type="SMR" id="Q3IUB7"/>
    <dbReference type="STRING" id="348780.NP_0346A"/>
    <dbReference type="EnsemblBacteria" id="CAI48264">
        <property type="protein sequence ID" value="CAI48264"/>
        <property type="gene ID" value="NP_0346A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702668"/>
    <dbReference type="KEGG" id="nph:NP_0346A"/>
    <dbReference type="eggNOG" id="arCOG02846">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017633_0_7_2"/>
    <dbReference type="OrthoDB" id="8967at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.230.10:FF:000002">
        <property type="entry name" value="Molecular chaperone DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000004">
        <property type="entry name" value="Molecular chaperone DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01152">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012724">
        <property type="entry name" value="DnaJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02349">
        <property type="entry name" value="DnaJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008035">
        <property type="entry name" value="PRK10767.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096">
        <property type="entry name" value="DNAJ HOMOLOG 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096:SF52">
        <property type="entry name" value="DNAJ HOMOLOG 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0346">Stress response</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_1000085230" description="Chaperone protein DnaJ">
        <location>
            <begin position="1"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="1">
        <location>
            <begin position="4"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif">
        <location>
            <begin position="154"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif">
        <location>
            <begin position="171"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif">
        <location>
            <begin position="197"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif">
        <location>
            <begin position="211"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="1">
        <location>
            <begin position="141"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="27"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="27"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="88"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="115"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="380" mass="41816" checksum="D76E4F02627D889B" modified="2005-11-08" version="1">MSEDFYSVLGVSRDADEDEIKQAYRKKASEYHPDVSDDPNAEEKFKQVKKAKEVLLDDEKRRMYDQMGHERFQEAEKRGATDTDRGRGGMGGMGGGGMGGMNDIFEQFFGGGGRSQSRSGPRQGSDLKTRLKVDLEDAYHGVTKQLTVTRPEECPDCDGAGHPPDADSRTCSACDGRGQQTTVRQTALGRVQQTRECPQCDGKGTIYSETCSTCRGDGQVRNETTLQVEVPAGIRDGQTLRMDGEGAPGENGGRKGDLLVEIRIDDHETFERDGDDLRCRHPISFPQAVFGDTVEVPTLDGAVEMDVPAGTQSGETFRLRGKGMPRLKRRGHGDLYVQVRVVTPESLNEEQREALEAFAEAGGEEIDVEQGFFEKLKSSF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-09-05" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IUJ6</accession>
    <name>RL18A_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein eL20</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L18Ae</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">50S ribosomal protein L20e</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">50S ribosomal protein LX</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl18a</name>
        <name evidence="1" type="synonym">rpl20e</name>
        <name evidence="1" type="synonym">rplX</name>
        <name type="ordered locus">NP_0186A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Binds 23S rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL20 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321823.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IUJ6"/>
    <dbReference type="SMR" id="Q3IUJ6"/>
    <dbReference type="STRING" id="348780.NP_0186A"/>
    <dbReference type="EnsemblBacteria" id="CAI48184">
        <property type="protein sequence ID" value="CAI48184"/>
        <property type="gene ID" value="NP_0186A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703149"/>
    <dbReference type="KEGG" id="nph:NP_0186A"/>
    <dbReference type="eggNOG" id="arCOG04175">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_177460_1_0_2"/>
    <dbReference type="OrthoDB" id="191241at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00273">
        <property type="entry name" value="Ribosomal_eL20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028877">
        <property type="entry name" value="Ribosomal_eL20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023573">
        <property type="entry name" value="Ribosomal_eL20_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001981">
        <property type="entry name" value="PRK00773.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01775">
        <property type="entry name" value="Ribosomal_L18A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160374">
        <property type="entry name" value="RplX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000419091" description="Large ribosomal subunit protein eL20">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="57" mass="6547" checksum="5CD168C23F4340B0" modified="2005-11-08" version="1">MSEFTVRGRFPARYGEQNFEKNVEAPNEDVAQERVYANFGSQHGLKRTQITIDEVDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IUQ4</accession>
    <name>Q3IUQ4_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase</fullName>
            <ecNumber evidence="9">4.1.3.44</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">tRNA wyosine derivatives biosynthesis protein Taw1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 11" type="primary">taw1</name>
        <name evidence="11" type="ordered locus">NP_0070A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the wyosine derivatives biosynthesis pathway that catalyzes the condensation of N-methylguanine with 2 carbon atoms from pyruvate to form the tricyclic 4-demethylwyosine (imG-14) on guanosine-37 of tRNA(Phe).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>N(1)-methylguanosine(37) in tRNA(Phe) + pyruvate + S-adenosyl-L-methionine = 4-demethylwyosine(37) in tRNA(Phe) + 5'-deoxyadenosine + L-methionine + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:36347"/>
            <dbReference type="Rhea" id="RHEA-COMP:10164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10165"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64315"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="EC" id="4.1.3.44"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the TYW1 family.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.44" evidence="9"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48126.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IUQ4"/>
    <dbReference type="STRING" id="348780.NP_0070A"/>
    <dbReference type="EnsemblBacteria" id="CAI48126">
        <property type="protein sequence ID" value="CAI48126"/>
        <property type="gene ID" value="NP_0070A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_0070A"/>
    <dbReference type="eggNOG" id="arCOG04174">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007952_3_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102521">
        <property type="term" value="F:tRNA-4-demethylwyosine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01921">
        <property type="entry name" value="TYW1_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013917">
        <property type="entry name" value="tRNA_wybutosine-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034556">
        <property type="entry name" value="tRNA_wybutosine-synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023993">
        <property type="entry name" value="TYW1_archaea"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03972">
        <property type="entry name" value="rSAM_TYW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930:SF0">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE TYW1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08608">
        <property type="entry name" value="Wyosine_form"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00284">
        <property type="entry name" value="tRNA_wybutosine-synthesizing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01071">
        <property type="entry name" value="tRNA_wybutosine-synthesizing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0408">Iron</keyword>
    <keyword evidence="6 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 9 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 9" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Radical SAM core" evidence="10">
        <location>
            <begin position="40"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI48126.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="321" mass="36326" checksum="53D684E1F60B3383" modified="2012-10-03" version="2">MPKQVGDPDYHSENHTAAQTCGWTANALRGEGRCYKNHFYGIQSHRCIQMTPVVKCNERCVFCWRDHAGHAYELGDVEWDDPEAVADASIELQRKLLSGFGGNDEVPRDVFEEAMEPRHVAISLDGEPTLYPYLPELIEAFHDRDITTFLVSNGTRPEVLAECDPTQLYVSVDAPDRKTFGEVVKAVEDDAWDRLIETLDVLAAKDETRTVLRTTLLNGYNMTNPAWYAAMVDRADADFVELKAFMHVGNSRDRLDREVMPDHEAVVEFTESIQSFLSEFPVLRDIEASRVALLARDEDTWVPELKGGSEFWTDGTYAKSD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IUT3</accession>
    <name>G3P_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glyceraldehyde-3-phosphate dehydrogenase</fullName>
            <shortName evidence="1">GAPDH</shortName>
            <ecNumber evidence="1">1.2.1.59</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gap</name>
        <name type="ordered locus">NP_0012A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glyceraldehyde 3-phosphate + phosphate + NADP(+) = (2R)-3-phospho-glyceroyl phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10296"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="1.2.1.59"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = (2R)-3-phospho-glyceroyl phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10300"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="1.2.1.59"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.59" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321736.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IUT3"/>
    <dbReference type="SMR" id="Q3IUT3"/>
    <dbReference type="STRING" id="348780.NP_0012A"/>
    <dbReference type="EnsemblBacteria" id="CAI48097">
        <property type="protein sequence ID" value="CAI48097"/>
        <property type="gene ID" value="NP_0012A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702737"/>
    <dbReference type="KEGG" id="nph:NP_0012A"/>
    <dbReference type="eggNOG" id="arCOG00493">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069533_0_0_2"/>
    <dbReference type="OrthoDB" id="295712at2157"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00184"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004365">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047100">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18127">
        <property type="entry name" value="GAPDH_II_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02278">
        <property type="entry name" value="GAPDH_II_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00559">
        <property type="entry name" value="G3P_dehdrog_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020831">
        <property type="entry name" value="GlycerAld/Erythrose_P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020830">
        <property type="entry name" value="GlycerAld_3-P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020829">
        <property type="entry name" value="GlycerAld_3-P_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020828">
        <property type="entry name" value="GlycerAld_3-P_DH_NAD(P)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006436">
        <property type="entry name" value="Glyceraldehyde-3-P_DH_2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01546">
        <property type="entry name" value="GAPDH-II_archae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003251">
        <property type="entry name" value="PRK04207.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02800">
        <property type="entry name" value="Gp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000149">
        <property type="entry name" value="GAP_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00846">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00071">
        <property type="entry name" value="GAPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000232393" description="Glyceraldehyde-3-phosphate dehydrogenase">
        <location>
            <begin position="1"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="12"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="140"/>
            <end position="142"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="195"/>
            <end position="196"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00559"/>
        </source>
    </evidence>
    <sequence length="338" mass="36122" checksum="9B67D5F6B9C526E7" modified="2005-11-08" version="1">MSIQVAVNGYGTIGKRVADAVTLQPDMELVGVAKTSPNHEAELAVENDYPLYAAIEDRIDDFEAAGIDLAGTVDELVEAADIVVDACPSGIGADNKSLYEAHDTPALYQGGESADLVDVSFNARSNFEAAADADHVRVVSCNTTGLSRLLAPLQEEYGVEKARVTLVRRGGDPGQTGRGPINDILPNPVSLPSHHGPDVNTIFPDLDIDTLGLKVPATLMHTHSVNVTLESTPDAEAVADLLDEQDRTFLIPESYGIDGAGKLKEFAMDRGRPRADIWENCIWAESVSMEGSDLYLFQAIHQESDVVPENIDAIRAVLGTADADESRETTNETLGVGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETG0</accession>
    <name>A0A1U7ETG0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ArNOG04375 family protein (Homolog to PilT-type ATPase)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">NP_0198A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the PINc/VapC protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321829.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETG0"/>
    <dbReference type="STRING" id="348780.NP_0198A"/>
    <dbReference type="EnsemblBacteria" id="CAI48190">
        <property type="protein sequence ID" value="CAI48190"/>
        <property type="gene ID" value="NP_0198A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700786"/>
    <dbReference type="KEGG" id="nph:NP_0198A"/>
    <dbReference type="eggNOG" id="arCOG04116">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023387_0_0_2"/>
    <dbReference type="OrthoDB" id="7146at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00105">
        <property type="entry name" value="KH-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09878">
        <property type="entry name" value="PIN_VapC_VirB11L-ATPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052041">
        <property type="entry name" value="Nucleic_acid_metab_PIN/TRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010335">
        <property type="entry name" value="PRK13764.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11603">
        <property type="entry name" value="AAA FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11603:SF147">
        <property type="entry name" value="MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="Prokaryotic type KH domain (KH-domain type II)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="PIN" evidence="6">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="257"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="484"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="525"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00046345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="617" mass="66796" checksum="6D2610EB7ED8A123" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETG1</accession>
    <name>A0A1U7ETG1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nitrilase</fullName>
            <ecNumber evidence="3">3.5.5.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">nitB</name>
        <name evidence="3" type="ordered locus">NP_0178A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the carbon-nitrogen hydrolase superfamily. Nitrilase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.5.1" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321819.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETG1"/>
    <dbReference type="STRING" id="348780.NP_0178A"/>
    <dbReference type="EnsemblBacteria" id="CAI48180">
        <property type="protein sequence ID" value="CAI48180"/>
        <property type="gene ID" value="NP_0178A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702497"/>
    <dbReference type="KEGG" id="nph:NP_0178A"/>
    <dbReference type="eggNOG" id="arCOG00126">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030130_6_2_2"/>
    <dbReference type="OrthoDB" id="198222at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000257">
        <property type="term" value="F:nitrilase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07564">
        <property type="entry name" value="nitrilases_CHs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044149">
        <property type="entry name" value="Nitrilases_CHs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46044:SF1">
        <property type="entry name" value="CN HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46044">
        <property type="entry name" value="NITRILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="2">
        <location>
            <begin position="6"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="367" mass="39957" checksum="3E0CECDAFB4D1E6A" modified="2017-05-10" version="1">MPAESFTLAAAQIEPVYHDKAATLDKTCRYIERAGNAGADLVVFPETYFPGYPYWRGSVSIPRWTELMVDLQKNSLHVDDEAVDVLAEAAADANVHVALGTNERSDRPGSETLYNSIFYFSRDGDLLGRHRKLMPTQEERAIWGRGDPSSLDTYETDIGTLGGLVCYENHMTLSKAALTAMGEEIHAAVWPGFWEQHGHPGDKSRAESSEAVDTCDIYPAMREYAFETQSFVAACSAYMGEDIPDGFSESELGFNVAAGGSMLINPAGIVKAGPLVGEEGLLTADFERDERRATKAYFDAMGHYTRWDAVSLSISDETLSPTVETGDVIRDNGTLSAAAAEAIADDHDIDTEAVEAVADAILDRHQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETG5</accession>
    <name>A0A1U7ETG5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Probable PAP2-type phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0190A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.3.-" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321825.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETG5"/>
    <dbReference type="STRING" id="348780.NP_0190A"/>
    <dbReference type="EnsemblBacteria" id="CAI48186">
        <property type="protein sequence ID" value="CAI48186"/>
        <property type="gene ID" value="NP_0190A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700783"/>
    <dbReference type="KEGG" id="nph:NP_0190A"/>
    <dbReference type="eggNOG" id="arCOG03058">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061498_0_0_2"/>
    <dbReference type="OrthoDB" id="10182at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969:SF13">
        <property type="entry name" value="AT30094P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="191"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="215"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="241"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="269"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="2">
        <location>
            <begin position="59"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="296" mass="29908" checksum="23CC6DCEB82C78C7" modified="2017-05-10" version="1">MSRAIGETATVAGLPEWVVSAAVVVTALGDVWFVFLFLGVLYWFGSALPGPVSLSRPHSAFAVALALGGLALTTALKEWLRLPRPAGAAEPTAAGLLTMLPPSAHAQVAAADGFGFPSGHAVAAIVVYGGLALLIDSRRGYAVAGLLCLLIPLSRVVIGVHYLVDILVGLALGGGYLAAVYRLCGRGDAPGRAMMIALLVAVVGAAIHYGQDTMLALGGTLGARIAWGIVGGAVAHEETTLLGGAVSTAVGLAFASGFAALYFVEPAPYVSFLGMSVVVAGVLVAPLVGEAVTRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETI3</accession>
    <name>A0A1U7ETI3_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAD-dependent urate hydroxylase HpyO/Asp monooxygenase CreE-like FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0226A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321843.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETI3"/>
    <dbReference type="STRING" id="348780.NP_0226A"/>
    <dbReference type="EnsemblBacteria" id="CAI48204">
        <property type="protein sequence ID" value="CAI48204"/>
        <property type="gene ID" value="NP_0226A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700793"/>
    <dbReference type="KEGG" id="nph:NP_0226A"/>
    <dbReference type="eggNOG" id="arCOG11370">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014633_2_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038732">
        <property type="entry name" value="HpyO/CreE_NAD-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38663">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38663:SF1">
        <property type="entry name" value="L-ORNITHINE N(5)-MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13454">
        <property type="entry name" value="NAD_binding_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-dependent urate hydroxylase HpyO/Asp monooxygenase CreE-like FAD/NAD(P)-binding" evidence="1">
        <location>
            <begin position="5"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="399" mass="42599" checksum="BDAC8344FD746942" modified="2017-05-10" version="1">MNEFVIVGGGVHGTYLANRATAEYDPEDITIVDPAPRLLSTFRQRAARCGMTELRSPFVHHLGRDPFDLETFAHSRGREAELVPTPNYPPRPTLSLFCDHAAHVIARRGLDALHRRGTVTDVRDAGDGLVVETTGGTLRSRRVVLAVGHGPPSLPEWAGDAIDHVWDDAPNSGRTASPRAVAADGGNVVADGDGVAENTNDKAPDIVVGGGSTAAQVALQRNADILLSRHPLRTSVTEAEPPWVNWPHIQRELHGHPPGSKARLKTVREARKSGTIRPQLRDRLEASDVSVVVGEVESAVETDDAVRLRLTDGRPLAGDAVCCATGFEPPWDRPFVRRLASSLGLERGYDGMPVLDDATVSWRGDTDGIYASGALAAGTVGPFAGNVIGARRAADRIVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETM4</accession>
    <name>A0A1U7ETM4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MiaB-like tRNA modifying enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0352A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321905.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETM4"/>
    <dbReference type="STRING" id="348780.NP_0352A"/>
    <dbReference type="EnsemblBacteria" id="CAI48267">
        <property type="protein sequence ID" value="CAI48267"/>
        <property type="gene ID" value="NP_0352A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700827"/>
    <dbReference type="KEGG" id="nph:NP_0352A"/>
    <dbReference type="eggNOG" id="arCOG00991">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116577_0_0_2"/>
    <dbReference type="OrthoDB" id="7576at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21149">
        <property type="entry name" value="PUA_archaeosine_TGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.90">
        <property type="entry name" value="ArcTGT, C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029402">
        <property type="entry name" value="TGT_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038250">
        <property type="entry name" value="TGT_C2_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14810">
        <property type="entry name" value="TGT_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88802">
        <property type="entry name" value="Pre-PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PUA" evidence="1">
        <location>
            <begin position="79"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="154" mass="16572" checksum="C63A34267980BD84" modified="2017-05-10" version="1">MSDERDVDALRTVADYQFGRGAGEALFDGEVEIQRTGSGRPQQVLVDGERVVSYGTDGRVTLSAVGGKRLQTALSPPAYRVIVGDDSEPFVRDEKNVFAKFVREVDPAIRPGDEVLVEHYDGDLLAVGRAELSAAAMADFETGMAVAVRDGNPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETM7</accession>
    <name>A0A1U7ETM7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0372A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321915.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETM7"/>
    <dbReference type="STRING" id="348780.NP_0372A"/>
    <dbReference type="EnsemblBacteria" id="CAI48277">
        <property type="protein sequence ID" value="CAI48277"/>
        <property type="gene ID" value="NP_0372A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700833"/>
    <dbReference type="KEGG" id="nph:NP_0372A"/>
    <dbReference type="eggNOG" id="arCOG03105">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_770755_0_0_2"/>
    <dbReference type="OrthoDB" id="166534at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043901">
        <property type="entry name" value="DUF5787"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19100">
        <property type="entry name" value="DUF5787"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="301"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="330" mass="38143" checksum="74EEB30A0691F2CC" modified="2017-05-10" version="1">MKEFGFELRVCAWAERHWQPSGDGRVLVARQLGTRSRRWDTVIIETTETALTQRAAFGPERLDSDLLFVAQHAPTGWDYYRDCLPDPGYPWRYVRESIHRAADRGFIETRRDGNRIEIRRRYRYPDWVDRIVAIENKPDLDASAARRLAEQLEHDVALGLADEVWLATNTTGDDIEPALLEAMPVDVGILAVDADGIHAERVWNPRTLDASSPGVRPTDRCDGRIDFEYADADWKQRKRLEIAERAYERGWRSYAETMRPDCRHFELRDPAGPFPWCAAKEREPTATECSGRCVDFEPEPPAWRSKEWPLSGGPGRAVSQLLDRKRQRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETP5</accession>
    <name>A0A1U7ETP5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Rhodanese domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0390A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321924.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETP5"/>
    <dbReference type="STRING" id="348780.NP_0390A"/>
    <dbReference type="EnsemblBacteria" id="CAI48286">
        <property type="protein sequence ID" value="CAI48286"/>
        <property type="gene ID" value="NP_0390A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700839"/>
    <dbReference type="KEGG" id="nph:NP_0390A"/>
    <dbReference type="eggNOG" id="arCOG02021">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089574_13_3_2"/>
    <dbReference type="OrthoDB" id="135517at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00158">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050229">
        <property type="entry name" value="GlpE_sulfurtransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43031">
        <property type="entry name" value="FAD-DEPENDENT OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43031:SF16">
        <property type="entry name" value="OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="16"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="116" mass="12601" checksum="560DB113C7F4655C" modified="2017-05-10" version="1">MDGEISAETLQELLDSDTDLRIVDIRSPAQFGRGHIPGSDNIPFQELTTHIEQVADADRVVTVCPHGQASQQAARLIASFEGFDGRVDSLEPGIEGWPYDLETDEGSETEASDAPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETS0</accession>
    <name>A0A1U7ETS0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF296 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0478A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321966.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETS0"/>
    <dbReference type="STRING" id="348780.NP_0478A"/>
    <dbReference type="EnsemblBacteria" id="CAI48330">
        <property type="protein sequence ID" value="CAI48330"/>
        <property type="gene ID" value="NP_0478A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700870"/>
    <dbReference type="KEGG" id="nph:NP_0478A"/>
    <dbReference type="eggNOG" id="arCOG04212">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114051_2_0_2"/>
    <dbReference type="OrthoDB" id="371648at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd11378">
        <property type="entry name" value="DUF296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.80">
        <property type="entry name" value="Hypothetical protein, similar to alpha- acetolactate decarboxylase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025707">
        <property type="entry name" value="DNA_bp_PD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005175">
        <property type="entry name" value="PPC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34988:SF1">
        <property type="entry name" value="DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34988">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03479">
        <property type="entry name" value="PCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016702">
        <property type="entry name" value="DNA_bp_PD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117856">
        <property type="entry name" value="AF0104/ALDC/Ptd012-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51742">
        <property type="entry name" value="PPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPC" evidence="1">
        <location>
            <begin position="6"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="140" mass="16124" checksum="7468D4A97C8AE968" modified="2017-05-10" version="1">MDYQRVEGDREYVARLEHGRDWREQIEAFAAEEDIDAAFFLGLGAVQDATVYFYDQVEQEYYPVEFDEPFEVTTALGNISHLDGERFAHTHVTLSREDGSMVGGHLDSATTFAGELYVRTFDAHLEREHDETTDLDLWPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETS7</accession>
    <name>A0A1U7ETS7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Homolog to PHA granule-associated 12K protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0486A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48334.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939418.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETS7"/>
    <dbReference type="EnsemblBacteria" id="CAI48334">
        <property type="protein sequence ID" value="CAI48334"/>
        <property type="gene ID" value="NP_0486A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700872"/>
    <dbReference type="KEGG" id="nph:NP_0486A"/>
    <dbReference type="eggNOG" id="arCOG03937">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2257318_0_0_2"/>
    <dbReference type="OrthoDB" id="6319at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89447">
        <property type="entry name" value="AbrB/MazE/MraZ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51740">
        <property type="entry name" value="SPOVT_ABRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="1">
        <location>
            <begin position="65"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48334.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="108" mass="11699" checksum="BDC939FC7DF030F6" modified="2017-05-10" version="1">MSDDDSGQDGIMWPPNLLKTVQETSEQATKKQQEALQQLLLGGGTATPDLGDLSDQIGTMSQMATFKTRVQSGGRVSIPDAEREALDIEEGDIVQAVVVPVKRKRDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETT2</accession>
    <name>A0A1U7ETT2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Probable oxidoreductase (Short-chain dehydrogenase family)</fullName>
            <ecNumber evidence="3">1.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0502A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321978.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETT2"/>
    <dbReference type="STRING" id="348780.NP_0502A"/>
    <dbReference type="EnsemblBacteria" id="CAI48342">
        <property type="protein sequence ID" value="CAI48342"/>
        <property type="gene ID" value="NP_0502A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700875"/>
    <dbReference type="KEGG" id="nph:NP_0502A"/>
    <dbReference type="eggNOG" id="arCOG01261">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_2"/>
    <dbReference type="OrthoDB" id="7442at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760:SF133">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="245" mass="26041" checksum="0EBBDEDBBFDB6E82" modified="2017-05-10" version="1">MLSPDCAGRTALVTGSARGVGRAFALALADAGADVAVHYNTSEAAAEATAEEARERDVDAVAVSADITSEEAVDGCFDTIEETLGPVDILVNNVGDFAPEHWADIDIETWRRVIETNLTGTMLCSKRALGPMRGSEWGRIVNVGYAAAERAAVAPKNFPYFVAKTGVLMFTRMLAADTTDDGITVNAVSPYVVETSDAFPETAPRGRWAAPDDLVQAMLFFVDEDSGYISGENIEVDGGWLPESV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU01</accession>
    <name>A0A1U7EU01_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0644A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
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        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
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    </evidence>
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        <source>
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    <sequence length="76" mass="8743" checksum="AF704BAD693C6D45" modified="2017-05-10" version="1">MYITLFTRKVCTSTKLVWSYGYACDGLTQNREEYPAAAILRRVEPSMRSTAAVDIGAVRPDKQPRTDVYRLICRRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <end position="95"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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            <dbReference type="EMBL" id="CAI48451.2"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="95" mass="10383" checksum="70DFC9F8B6756220" modified="2017-05-10" version="1">MRLLANVEERIDAHSYPATTEELVEEYGDLELELPNGEETFGDALGRLGETTFEDAEDARLAAYSAVSSNAIGRQNYSDRDAPSIGENGPEQVSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU33</accession>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <dbReference type="EMBL" id="CAI48458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="236" mass="24291" checksum="89B55DEBCD88538F" modified="2017-05-10" version="1">MTDTLLLVGRAHVSAVAQRHAARLRERGVADAVAVATYDEDPAELAPAVEGTPYVVPLAAAPGRDTMQSIPGALPAARHCAPVGTNPAVTRALADRAREALSPGADASVALVAFGDASGTDARQTAEAHAERLRSATAYGEVRTAYLLQNPAAECVRYNLTGERGVAVPFFVAACEETDRALPAKLDLDRGGLAYADPLGDHKAVTDAIEAEFERARAAGSSTAAVSPVATDGRGP</sequence>
</entry>
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    <accession>A0A1U7EU36</accession>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <location>
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            <end position="67"/>
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            <begin position="88"/>
            <end position="111"/>
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        <location>
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        <location>
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            <end position="194"/>
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    </feature>
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        <location>
            <begin position="200"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI48442.1"/>
        </source>
    </evidence>
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        <source>
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</entry>
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    <accession>A0A1U7EU48</accession>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="53" mass="6193" checksum="B657D40AB9E4CA5D" modified="2017-05-10" version="1">MQCHYCDEEAAVAVEKDHVKVGLCKPHLRERMDELSDSEWVEEFQSQIDDALE</sequence>
</entry>
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    <accession>A0A1U7EU64</accession>
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        <name evidence="3" type="primary">sufS2</name>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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    <dbReference type="KEGG" id="nph:NP_0790A"/>
    <dbReference type="eggNOG" id="arCOG00065">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003433_2_1_2"/>
    <dbReference type="OrthoDB" id="9577at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031071">
        <property type="term" value="F:cysteine desulfurase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43586">
        <property type="entry name" value="CYSTEINE DESULFURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43586:SF8">
        <property type="entry name" value="CYSTEINE DESULFURASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="2">
        <location>
            <begin position="17"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="364" mass="39038" checksum="C4A3E67863393EEF" modified="2017-05-10" version="1">MEFDELRADIPAAASHVYLNTGASGPSPRRVVEAVNDAQRRQEFDAGATNHYETAAEIRETARETVAGYLGCSADSIALVESTGDGISRVANAIEWSAGDRVVRTDLEHPSGVLPWRRREEQGVDVDVLSCPDGRLPMDAYREAVEDAAVVCLSSESWLHGTRLAVEEAVDIAHAAGALVVVDAVQTVGQHPVDVREWGADVVCASGHKWLLGPWGAGFLYVDPDALETFRPRHIGYRSVTDPTGDGLEYAPDATRFGVSTGAVAPYAGLVEAIETFEEIGFDAIEAEIERLTDRLKDRLGDRLVSPTDYESGLVAFEVAEPASFVERAADAGIVVRDLPNGAARASIHAFNTDDDIDALAALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU98</accession>
    <name>A0A1U7EU98_NATPD</name>
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        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_0850A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322152.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU98"/>
    <dbReference type="STRING" id="348780.NP_0850A"/>
    <dbReference type="EnsemblBacteria" id="CAI48516">
        <property type="protein sequence ID" value="CAI48516"/>
        <property type="gene ID" value="NP_0850A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700975"/>
    <dbReference type="KEGG" id="nph:NP_0850A"/>
    <dbReference type="eggNOG" id="arCOG02827">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_800808_0_0_2"/>
    <dbReference type="OrthoDB" id="235883at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055564">
        <property type="entry name" value="CdpA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055563">
        <property type="entry name" value="CdpA_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23601">
        <property type="entry name" value="CdpA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23600">
        <property type="entry name" value="CdpA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Cell division protein A N-terminal" evidence="3">
        <location>
            <begin position="3"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Cell division protein A C-terminal" evidence="4">
        <location>
            <begin position="303"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="231"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="288"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI48516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="346" mass="35554" checksum="64133054C433C6FA" modified="2017-05-10" version="1">MASLTEVYEGGSGAGLRRLYAGVVLFGVGVVLVTTGILIASTGLGAYVGLGLYEAREVAGILGGVGLPAVLLGMLVALPRAARELQVGVGAGAVLCLIGVGMFRRWYPVDWVGASGNTTMTLVVAATYFAGAIVMTWSLFTAVANFKARNDPGGTVKLEVTKGGETKVVEVSNDDLRGTLGGIGLLGGQPEGDVETQTNDGSRDRNRNKQATGGGAGVGSRSSDTRRRTESGSLSPRSSGGSGSPSRSSSRGSSSRSRQSRSAPGPAADASATDGGATDEPSIQTPGDDAEFLDDGPETPSNDRYCGNCTHFRYARTDDGLVPYCGLHSEAMDDMDACDKWSPNTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUA7</accession>
    <name>A0A1U7EUA7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Abi/CAAX domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0912A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322183.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUA7"/>
    <dbReference type="STRING" id="348780.NP_0912A"/>
    <dbReference type="EnsemblBacteria" id="CAI48547">
        <property type="protein sequence ID" value="CAI48547"/>
        <property type="gene ID" value="NP_0912A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700997"/>
    <dbReference type="KEGG" id="nph:NP_0912A"/>
    <dbReference type="eggNOG" id="arCOG02770">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061949_0_0_2"/>
    <dbReference type="OrthoDB" id="214851at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080120">
        <property type="term" value="P:CAAX-box protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052710">
        <property type="entry name" value="CAAX_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1/LyrA-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36435:SF1">
        <property type="entry name" value="CAAX AMINO TERMINAL PROTEASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36435">
        <property type="entry name" value="SLR1288 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="219"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="CAAX prenyl protease 2/Lysostaphin resistance protein A-like" evidence="2">
        <location>
            <begin position="161"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="264" mass="26609" checksum="FCEB65FBFFEE143A" modified="2017-05-10" version="1">MTRWVAFAALTAAVVGVMLAAARASTRALSTTDTADTAAGLESLPDGADHLDATTAERQHPSSTPSPDALPTQALLLNLAVSHGFLAAVLAAGIWLANVPLASLGVGAGPVTGSIAVAVGLAVGAAIAVVNTLLSVVASRLNVDPSEQLRSLLAPETTLDWALLCFVVFPIVAGFEELLFRGILVGAFAVGFDLSPWLLAVVSSALFAAGHGAQGRLGVVATGVLGFVLAAVFISTGSLLVVAVAHYVVNVVEFLVAEGLGWTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUE0</accession>
    <name>A0A1U7EUE0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0988A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322220.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUE0"/>
    <dbReference type="STRING" id="348780.NP_0988A"/>
    <dbReference type="EnsemblBacteria" id="CAI48585">
        <property type="protein sequence ID" value="CAI48585"/>
        <property type="gene ID" value="NP_0988A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701022"/>
    <dbReference type="KEGG" id="nph:NP_0988A"/>
    <dbReference type="eggNOG" id="arCOG08115">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2505048_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="85" mass="8462" checksum="A138222103760F10" modified="2017-05-10" version="1">MVRKDSKFAIGVLNAFGALASAILAALVMLVFVILSLFVTVFVVDVAASIGGLNPDDGFVVLGATILAGAAILAGGVGFATPEEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUG7</accession>
    <name>A0A1U7EUG7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Small CPxCG-related zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_1058A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48620.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322256.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUG7"/>
    <dbReference type="STRING" id="348780.NP_1058A"/>
    <dbReference type="EnsemblBacteria" id="CAI48620">
        <property type="protein sequence ID" value="CAI48620"/>
        <property type="gene ID" value="NP_1058A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701043"/>
    <dbReference type="KEGG" id="nph:NP_1058A"/>
    <dbReference type="eggNOG" id="arCOG06320">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2930290_0_0_2"/>
    <dbReference type="OrthoDB" id="157634at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055995">
        <property type="entry name" value="DUF7573"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24458">
        <property type="entry name" value="DUF7573"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7573" evidence="2">
        <location>
            <begin position="22"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48620.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="60" mass="6292" checksum="8332AF78A78E4EC8" modified="2017-05-10" version="1">MRETSLNDFADGAADGSSDDTASATYVWTPSDAACDGCGVSVARRWRAADGLVCADCKDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUJ5</accession>
    <name>A0A1U7EUJ5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Stomatin family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">NP_1126A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the band 7/mec-2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48654.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939447.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUJ5"/>
    <dbReference type="STRING" id="348780.NP_1126A"/>
    <dbReference type="EnsemblBacteria" id="CAI48654">
        <property type="protein sequence ID" value="CAI48654"/>
        <property type="gene ID" value="NP_1126A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701057"/>
    <dbReference type="KEGG" id="nph:NP_1126A"/>
    <dbReference type="eggNOG" id="arCOG01915">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024949_2_0_2"/>
    <dbReference type="OrthoDB" id="10752at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000004">
        <property type="entry name" value="Putative membrane protease family, stomatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050710">
        <property type="entry name" value="Band7/mec-2_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001972">
        <property type="entry name" value="Stomatin_HflK_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43327">
        <property type="entry name" value="STOMATIN-LIKE PROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43327:SF10">
        <property type="entry name" value="STOMATIN-LIKE PROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00721">
        <property type="entry name" value="STOMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="Band 7" evidence="7">
        <location>
            <begin position="29"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="332"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="347"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="359"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI48654.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="383" mass="42592" checksum="D8178663E430C934" modified="2017-05-10" version="1">MLTPLQIGETVIPVVALLLLVVAVVAVYQAVEIVDAYEKRALTVFGEYRRLLEPGINFVPPFVSRTYTFDMRTQTLDVPRQEAITRDNSPVTADAVVYIKVMDAKKAFLEVDNYKKAVSNLAQTTLRAVLGDMELDDTLNKRQEINAKIRKELDEPTDEWGIRVESVEVREVNPSKDVQQAMEQQTSAERKRRAMILEAQGERRSAIEKAEGDKQSNIIRAQGEKQSQILEAQGDAIGTVLRAKSAEAMGERAVIERGMETLEEIGKGESTTFVLPQELTSLLSRYGKHLTGSDAADQSQLLEGLEFDDEARELLGLDNIEEILGQIDEEAEVDPKEMEAEAQAIKSGEDPANIKDPDEVIAEMDEEMPEGGLDDADLERESN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUL7</accession>
    <name>A0A1U7EUL7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ArsA family ATPase</fullName>
            <ecNumber evidence="4">3.6.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">arsA1</name>
        <name evidence="4" type="synonym">tpa07</name>
        <name evidence="4" type="ordered locus">NP_1164A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arsA ATPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.-.-" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322309.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUL7"/>
    <dbReference type="STRING" id="348780.NP_1164A"/>
    <dbReference type="EnsemblBacteria" id="CAI48673">
        <property type="protein sequence ID" value="CAI48673"/>
        <property type="gene ID" value="NP_1164A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703354"/>
    <dbReference type="KEGG" id="nph:NP_1164A"/>
    <dbReference type="eggNOG" id="arCOG02849">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040761_2_1_2"/>
    <dbReference type="OrthoDB" id="46198at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02035">
        <property type="entry name" value="ArsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025723">
        <property type="entry name" value="ArsA/GET3_ATPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016300">
        <property type="entry name" value="ATPase_ArsA/GET3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00345">
        <property type="entry name" value="GET3_arsA_TRC40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803">
        <property type="entry name" value="ARSENICAL PUMP-DRIVING ATPASE ARSENITE-TRANSLOCATING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803:SF3">
        <property type="entry name" value="ATPASE GET3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02374">
        <property type="entry name" value="ArsA_ATPase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ArsA/GET3 Anion-transporting ATPase-like" evidence="3">
        <location>
            <begin position="25"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="ArsA/GET3 Anion-transporting ATPase-like" evidence="3">
        <location>
            <begin position="152"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="96"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="113"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="370" mass="39092" checksum="00E16EA263851CD8" modified="2017-05-10" version="1">MSDLSVEPIDSVDEADLPAGVDAPEYVLYGGKGGVGKTTCAAATALASARDGTPTLVVSTDPAHSLSDTLGVDVPDEPTRIAEETPLFAVEIDPETAAGPFAPGEDGPDADPLEGAAGPDGAAGPEDGGLGMGMGGLEDLLGGDGHPLAGGAMPGADEAAAVQLLLEYLDDPRFDRVVVDTAPTGHTLRLLELPDVMDSMVGRLLSFREKLSGMMGSVGGLFGGSDDPEAEMEAGMDDLQELSTKIERLRTALRDPDRTDFRVVMVPEEMSVVESERLVDRLEEFGIPVGTLVVNRVSEELADVASFDEEWFVAPDTENCAFCRRRWEVQRTALERAQGLFRGREIKRVPLFAEPVHGEEMLGVVGACLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUP6</accession>
    <name>A0A1U7EUP6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1240A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUP6"/>
    <dbReference type="STRING" id="348780.NP_1240A"/>
    <dbReference type="EnsemblBacteria" id="CAI48711">
        <property type="protein sequence ID" value="CAI48711"/>
        <property type="gene ID" value="NP_1240A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701092"/>
    <dbReference type="KEGG" id="nph:NP_1240A"/>
    <dbReference type="eggNOG" id="arCOG11882">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159952_0_0_2"/>
    <dbReference type="OrthoDB" id="206114at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="125" mass="12548" checksum="3BAA28C78E0F75B9" modified="2017-05-10" version="1">MTDRTQSVFTAGFVVGTLLSALGVGAWVLTDFASMTALIPALFGVLIIGFASVGRATDRERLGMYGIGALGALGVLGSLRAVPDIIALVTGGDGDSAVAATSQGLMIVLGLVLVAVVARAVITDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUP8</accession>
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        <recommendedName>
            <fullName evidence="3">KaiC domain protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="1" type="ordered locus">NP_1236A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
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            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUP8"/>
    <dbReference type="STRING" id="348780.NP_1236A"/>
    <dbReference type="EnsemblBacteria" id="CAI48709">
        <property type="protein sequence ID" value="CAI48709"/>
        <property type="gene ID" value="NP_1236A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701090"/>
    <dbReference type="KEGG" id="nph:NP_1236A"/>
    <dbReference type="eggNOG" id="arCOG02453">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1412400_0_0_2"/>
    <dbReference type="OrthoDB" id="191154at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055516">
        <property type="entry name" value="DUF7090"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23365">
        <property type="entry name" value="DUF7090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="192" mass="21794" checksum="5B68643B121E8166" modified="2017-05-10" version="1">MDYELAIENTPETVPAGTGILLIHPSTGETDRIDTDFFKTDTDRLFVISTRTTAREVQQKLEYYDVDESTAEILDTLSVERGYSRRSSDRVHYVSAPDDLDGIVETTRQFLDENEGKRRISLDSVTEMAYYADEERALEAVGELLELLAEYDAVGLFHLSEEVHDDADVEAFKELFDGIVYLEEDGTVESQF</sequence>
</entry>
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    <accession>A0A1U7EUR5</accession>
    <name>A0A1U7EUR5_NATPD</name>
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        <submittedName>
            <fullName evidence="2">UspA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1284A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
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    <dbReference type="STRING" id="348780.NP_1284A"/>
    <dbReference type="EnsemblBacteria" id="CAI48733">
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        <property type="gene ID" value="NP_1284A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701100"/>
    <dbReference type="KEGG" id="nph:NP_1284A"/>
    <dbReference type="eggNOG" id="arCOG06388">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134823_0_0_2"/>
    <dbReference type="OrthoDB" id="193961at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="1">
        <location>
            <begin position="2"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="147" mass="15933" checksum="AEBA87321B108C40" modified="2017-05-10" version="1">MTLVVPYDGSALAEAAIDRAEQFRSSVDEEIVVVTVIPNNESYARERGWLQDDEAFTLNAVVSTLRERTVASRDGVSFRYVTVGRYAATGTIASRIRTEIQRVGAEVVFIGSDNAGRVVTSIASVGSKVAAEDAYDIFIVRRPSEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUV1</accession>
    <name>A0A1U7EUV1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Oxidoreductase (Homolog to thioredoxin-disulfide reductase)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_1384A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
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            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322418.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUV1"/>
    <dbReference type="STRING" id="348780.NP_1384A"/>
    <dbReference type="EnsemblBacteria" id="CAI48783">
        <property type="protein sequence ID" value="CAI48783"/>
        <property type="gene ID" value="NP_1384A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701134"/>
    <dbReference type="KEGG" id="nph:NP_1384A"/>
    <dbReference type="eggNOG" id="arCOG01301">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068376_1_1_2"/>
    <dbReference type="OrthoDB" id="341719at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050097">
        <property type="entry name" value="Ferredoxin-NADP_redctase_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48105">
        <property type="entry name" value="THIOREDOXIN REDUCTASE 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="3">
        <location>
            <begin position="17"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="257" mass="27584" checksum="2D9235AE522508B7" modified="2017-05-10" version="1">MTAADTETGTDADPPRAAVIGGGVAGLTAATFLARAGLDTVVIDHGESILRRNAHLENVPGFPAGVNSRLFLDLLERQAERNGCLFEQGFVTAVEPGDDGFRLRIEADTEFDLAADYVIAASWADTAYLEPLEAVGLTERGSKTYVDTDDDGRTDLDGLYAAGRTAGAAHQTVVCAGDGADVALTLVHDSDVPFYHDWVAPDGYFTDRGREVPPGCEEIDEAERRRRERESMEVMAEAFAEAHPDEPTMHPSAADDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUY8</accession>
    <name>A0A1U7EUY8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thioredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">trxA2</name>
        <name evidence="2" type="ordered locus">NP_1448A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322450.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUY8"/>
    <dbReference type="SMR" id="A0A1U7EUY8"/>
    <dbReference type="STRING" id="348780.NP_1448A"/>
    <dbReference type="EnsemblBacteria" id="CAI48815">
        <property type="protein sequence ID" value="CAI48815"/>
        <property type="gene ID" value="NP_1448A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703423"/>
    <dbReference type="KEGG" id="nph:NP_1448A"/>
    <dbReference type="eggNOG" id="arCOG01972">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090389_10_4_2"/>
    <dbReference type="OrthoDB" id="35385at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45663">
        <property type="entry name" value="GEO12009P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45663:SF11">
        <property type="entry name" value="GEO12009P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="1">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="108" mass="11570" checksum="149AE506435BCB80" modified="2017-05-10" version="1">MDAAVVRHLDDERDFEAFVTSHEVVLVEFYTTGCPKCAAMEPVLGTVARATGVPVGLCDLGSVPVVERFDIASTPTLILFTNGDPADRLAEGFVGADRLVEFVDAVQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUY9</accession>
    <name>A0A1U7EUY9_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Conditioned medium-induced protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1392A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322422.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUY9"/>
    <dbReference type="STRING" id="348780.NP_1392A"/>
    <dbReference type="EnsemblBacteria" id="CAI48787">
        <property type="protein sequence ID" value="CAI48787"/>
        <property type="gene ID" value="NP_1392A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701136"/>
    <dbReference type="KEGG" id="nph:NP_1392A"/>
    <dbReference type="eggNOG" id="arCOG04725">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095571_0_0_2"/>
    <dbReference type="OrthoDB" id="146450at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="198" mass="21376" checksum="62DC12D91BBBAD4E" modified="2017-05-10" version="1">MDEKTESLRDIFVEATGTDTVTETQTETPGTLEPDGDVAERLAAVVADCREESAFSTGLSDTELAALVEGFYQGADDGELAAELSVPEATVVDARLDLRLVRDADTDIPVPLDTVAEAVEDDTAIAALADEAEVDTERLRRCVRALAAQARRRNESDRYRDRFEDILVDADLESSHTAGTTDDGLEDAIEGMEVDVPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV21</accession>
    <name>A0A1U7EV21_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">DNA polymerase</fullName>
            <ecNumber evidence="2 11">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">polB</name>
        <name evidence="14" type="ordered locus">NP_1508A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="2 11"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322479.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="STRING" id="348780.NP_1508A"/>
    <dbReference type="EnsemblBacteria" id="CAI48845">
        <property type="protein sequence ID" value="CAI48845"/>
        <property type="gene ID" value="NP_1508A"/>
    </dbReference>
    <dbReference type="GeneID" id="69102738"/>
    <dbReference type="KEGG" id="nph:NP_1508A"/>
    <dbReference type="eggNOG" id="arCOG00328">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000203_6_3_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016539">
        <property type="term" value="P:intein-mediated protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05160">
        <property type="entry name" value="DEDDy_DNA_polB_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00081">
        <property type="entry name" value="Hint"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.342.10">
        <property type="entry name" value="DNA Polymerase, chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.16.10">
        <property type="entry name" value="Hedgehog/Intein (Hint) domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="entry name" value="Homing endonucleases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050240">
        <property type="entry name" value="DNA_pol_type-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003587">
        <property type="entry name" value="Hint_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
        <property type="entry name" value="Hint_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004042">
        <property type="entry name" value="Intein_endonuc_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006141">
        <property type="entry name" value="Intein_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01445">
        <property type="entry name" value="intein_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322">
        <property type="entry name" value="DNA POLYMERASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322:SF23">
        <property type="entry name" value="DNA POLYMERASE DELTA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00306">
        <property type="entry name" value="HintN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51294">
        <property type="entry name" value="Hedgehog/intein (Hint) domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="Homing endonucleases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50819">
        <property type="entry name" value="INTEIN_ENDONUCLEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50817">
        <property type="entry name" value="INTEIN_N_TER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="11" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 11 14" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="5 11 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-0651">Protein splicing</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DOD-type homing endonuclease" evidence="13">
        <location>
            <begin position="733"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="domain" description="DOD-type homing endonuclease" evidence="13">
        <location>
            <begin position="1212"/>
            <end position="1353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="20"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAI48845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="1740" mass="196037" checksum="292FE2C7C790FFE2" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV22</accession>
    <name>A0A1U7EV22_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Proteasome-activating nucleotidase</fullName>
            <shortName evidence="9">PAN</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Proteasomal ATPase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Proteasome regulatory ATPase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Proteasome regulatory particle</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">pan2</name>
        <name evidence="9" type="synonym">pan</name>
        <name evidence="12" type="ordered locus">NP_1524A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">ATPase which is responsible for recognizing, binding, unfolding and translocation of substrate proteins into the archaeal 20S proteasome core particle. Is essential for opening the gate of the 20S proteasome via an interaction with its C-terminus, thereby allowing substrate entry and access to the site of proteolysis. Thus, the C-termini of the proteasomal ATPase function like a 'key in a lock' to induce gate opening and therefore regulate proteolysis. Unfolding activity requires energy from ATP hydrolysis, whereas ATP binding alone promotes ATPase-20S proteasome association which triggers gate opening, and supports translocation of unfolded substrates.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homohexamer. The hexameric complex has a two-ring architecture resembling a top hat that caps the 20S proteasome core at one or both ends. Upon ATP-binding, the C-terminus of PAN interacts with the alpha-rings of the proteasome core by binding to the intersubunit pockets.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="9">Consists of three main regions, an N-terminal coiled-coil domain that may assist in substrate recognition, an interdomain involved in PAN hexamerization, and a C-terminal ATPase domain of the AAA type.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 10">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322487.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV22"/>
    <dbReference type="STRING" id="348780.NP_1524A"/>
    <dbReference type="EnsemblBacteria" id="CAI48853">
        <property type="protein sequence ID" value="CAI48853"/>
        <property type="gene ID" value="NP_1524A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703008"/>
    <dbReference type="KEGG" id="nph:NP_1524A"/>
    <dbReference type="eggNOG" id="arCOG01306">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_2_0_2"/>
    <dbReference type="OrthoDB" id="77269at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022623">
        <property type="term" value="C:proteasome-activating nucleotidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043335">
        <property type="term" value="P:protein unfolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000033">
        <property type="entry name" value="26S protease regulatory subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000006">
        <property type="entry name" value="26S protease regulatory subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00553">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023501">
        <property type="entry name" value="Nucleotidase_PAN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003069">
        <property type="entry name" value="PRK03992.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF047748">
        <property type="entry name" value="PrtsmActNtasePan1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 9 12" id="KW-0647">Proteasome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="181"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="15"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="192"/>
            <end position="197"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI48853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="404" mass="45353" checksum="89C90C52E2FB6E31" modified="2017-05-10" version="1">MTDTVDDVDLPYEDGASQQEKLEALEERLEILEQQNEEMRDKLLDANAENNKYQQKLERLTHENKKLKQSPLFVATVQELTGEGVIIKQHGNNQEALTEVTDDMREDLAPDDRVAVNNSLSIVKRLDSETDVRARVMQVDESPDVSYEDIGGIDDQMEEVRETVEMPIESPEMFDEVGIDPPSGVLLHGPPGTGKTMLAKAVANQTDATFIKMAGSELVHKFIGEGAKLVRDLFELARQEVPAVVFIDEIDAIAAKRTESKTSGDAEVQRTMMQLLAEMDGFEDRGQISIIAATNRFDMLDRAILRPGRFDRLIEVPKPDFEGREIIFDIHTRDMNVADGVDYTELAEMADNASGADIKAICTEAGMYAIRDDRTEVRHEDFEQAWRKIQETEEADADVSKTFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV55</accession>
    <name>A0A1U7EV55_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">SCO1/SenC/PrrC family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_1604A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SCO1/2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322527.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV55"/>
    <dbReference type="STRING" id="348780.NP_1604A"/>
    <dbReference type="EnsemblBacteria" id="CAI48893">
        <property type="protein sequence ID" value="CAI48893"/>
        <property type="gene ID" value="NP_1604A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701198"/>
    <dbReference type="KEGG" id="nph:NP_1604A"/>
    <dbReference type="eggNOG" id="arCOG00313">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093041_0_0_2"/>
    <dbReference type="OrthoDB" id="27579at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02968">
        <property type="entry name" value="SCO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003782">
        <property type="entry name" value="SCO1/SenC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02630">
        <property type="entry name" value="SCO1-SenC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0186">Copper</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="3">
        <location>
            <begin position="97"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR603782-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR603782-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="227" mass="24865" checksum="2143587EB925B451" modified="2017-05-10" version="1">MSLPHNGYDRRTVLKAVGAAAVGTATAGCLSSADEAGTVLPPPENYDRRQDLDLPFPDYGEEIPEITVPAPLQDRDVTTTEFIGERHTMYTFVYTTCTTVCGALAATLQRVQIDSIESGYADEMAFLPMTFDPEVDTADVIREYEEGLGVAEDAGNWYFLRPEDSTEAEQVVDERFGVAFEGDGGHYQHTSLVLLVNKDGIVERAYNGDPPRSDIAISDATTVVEEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVA7</accession>
    <name>A0A1U7EVA7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">IS1341-type transposase ISNph20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">NP_1714A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322581.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVA7"/>
    <dbReference type="STRING" id="348780.NP_1714A"/>
    <dbReference type="EnsemblBacteria" id="CAI48948">
        <property type="protein sequence ID" value="CAI48948"/>
        <property type="gene ID" value="NP_1714A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701234"/>
    <dbReference type="KEGG" id="nph:NP_1714A"/>
    <dbReference type="eggNOG" id="arCOG00684">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_0_1_2"/>
    <dbReference type="OrthoDB" id="33505at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021027">
        <property type="entry name" value="Transposase_put_HTH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF11">
        <property type="entry name" value="RNA-GUIDED DNA ENDONUCLEASE RV2885C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12323">
        <property type="entry name" value="HTH_OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Transposase putative helix-turn-helix" evidence="11">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="9">
        <location>
            <begin position="186"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="10">
        <location>
            <begin position="302"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="214"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI48948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="419" mass="48024" checksum="F870341CB2428214" modified="2017-05-10" version="1">MNYNYRYRLKPTESQCETLDYHRDTCRQLYNHALYRFSQIPESEGTVKQRVRTIRDELPDLKDWWDALTDIYSKVLQPTVMRIAKNIKALGQLKEQGYKVGELRWKSPREFRSFTYNQSGFELDKKGGQTVLSLSKLADIPIELHRPLPDDATVKQVTLKKEKAGEWFAIFGMEMDAEPPAKPPLEDINIDEMVGIDVGILKYAHDTDGTAVESLDLSEERDRLEREQRKLSRKEHGSNNWENQRRRVAECHLQIKRKQRDFLHKLSNYYAREYELVAVEDLDVKDMLESPRNSRNTASAAWNTFTDMLEAKCEREGTHFVEVEPEGTTKECAQCGVETEKPLWVREHSCPACGFEADRDANAAWNILFRGLTDLGVGHSEGTPVETALPTDTHSVSAKRVVEAGSPTLSERVSASRVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVB0</accession>
    <name>A0A1U7EVB0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1708A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322578.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVB0"/>
    <dbReference type="STRING" id="348780.NP_1708A"/>
    <dbReference type="EnsemblBacteria" id="CAI48945">
        <property type="protein sequence ID" value="CAI48945"/>
        <property type="gene ID" value="NP_1708A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701231"/>
    <dbReference type="KEGG" id="nph:NP_1708A"/>
    <dbReference type="eggNOG" id="arCOG02976">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068381_1_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043829">
        <property type="entry name" value="DUF5806"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19122">
        <property type="entry name" value="DUF5806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="219" mass="24439" checksum="C130606C3535CDC5" modified="2017-05-10" version="1">MDDDARTAPDDDALGADAEAPTEDGPDGEQSDSAGTEADPAEDVPKDVQEYTRFSKIDGATYDRANEFLRDRTYITAREWAIARLCADFRTETGVEMTKIGENLPELVPFMTDTYSPQAVNQARSSFDEKVRKAGATFLYGAMCDFYTAEELDELMYEVTEIAKFLLEVEGVELTVDEELAAEDRISSVMREVRESSAALRHDELQCPNCGHEMETQDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVI4</accession>
    <name>A0A1U7EVI4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1874A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322660.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVI4"/>
    <dbReference type="STRING" id="348780.NP_1874A"/>
    <dbReference type="EnsemblBacteria" id="CAI49028">
        <property type="protein sequence ID" value="CAI49028"/>
        <property type="gene ID" value="NP_1874A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701285"/>
    <dbReference type="KEGG" id="nph:NP_1874A"/>
    <dbReference type="eggNOG" id="arCOG13694">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_827986_0_0_2"/>
    <dbReference type="OrthoDB" id="234780at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="334" mass="36607" checksum="527ACD0F72C5B9B3" modified="2017-05-10" version="1">MADNEPTQHGDSETPTITRRRLLAAGGGGALAAAGGGVVYNTQLGYGRFTGTNLRKQDLDPVVTERLDPTFDETLGEGHIRTDDAALVADGDRLPFDADRDAVASVDRHIAANDRLLALWEDLAALDRGEFTIESSTPSAFFDRIAGATARPETVTAIRGRWDQTVDVDLVERFAATDPADPRALVDGLADAFERHTDYDIPRYLAGAIEDNVLFGAVDLRSRFEAAVDFESLLADDPRIFCWELTYRAIEALHAVEAAEQTLPVAACYVSDRRHKHVYTGVVSAVRDEGTLVLPMTFLDYTAVLLDDTPGFSRLFGDGLAAYDRDHRADEIYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVJ5</accession>
    <name>A0A1U7EVJ5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HAD superfamily hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1896A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HAD-like hydrolase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322671.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVJ5"/>
    <dbReference type="STRING" id="348780.NP_1896A"/>
    <dbReference type="EnsemblBacteria" id="CAI49039">
        <property type="protein sequence ID" value="CAI49039"/>
        <property type="gene ID" value="NP_1896A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701296"/>
    <dbReference type="KEGG" id="nph:NP_1896A"/>
    <dbReference type="eggNOG" id="arCOG02291">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_8_3_2"/>
    <dbReference type="OrthoDB" id="27736at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008967">
        <property type="term" value="F:phosphoglycolate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050155">
        <property type="entry name" value="HAD-like_hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43434">
        <property type="entry name" value="PHOSPHOGLYCOLATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43434:SF1">
        <property type="entry name" value="PHOSPHOGLYCOLATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="233" mass="25353" checksum="772F407BF6670549" modified="2017-05-10" version="1">MTDDAAHATDTEAVLWDIGGVLVELRSVREGYAAFVAELAADAGRDPDAALETWQSVLGDHFRGREGNQYRLARDGYEKATAALFDGDPPADWLETFEAATKPALRPEPGAVETVEALAEAGYRQAIVSDIDTPEAHRMLEAFGIRDRFDHITTSEAVGYTKPDERMFQDALSALDVAPERAVMVGDRHSHDVTGAAALGIRTVGYGEEAWGDQADHEIEDLREILELLGVES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVL7</accession>
    <name>A0A1U7EVL7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha/beta hydrolase fold protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1966A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322704.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVL7"/>
    <dbReference type="STRING" id="348780.NP_1966A"/>
    <dbReference type="EnsemblBacteria" id="CAI49074">
        <property type="protein sequence ID" value="CAI49074"/>
        <property type="gene ID" value="NP_1966A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701323"/>
    <dbReference type="KEGG" id="nph:NP_1966A"/>
    <dbReference type="eggNOG" id="arCOG01652">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089548_0_0_2"/>
    <dbReference type="OrthoDB" id="50239at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005645">
        <property type="entry name" value="FSH-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03959">
        <property type="entry name" value="FSH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine hydrolase" evidence="1">
        <location>
            <begin position="75"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="203" mass="20893" checksum="045DA34B2C74BF42" modified="2017-05-10" version="1">MSKSVVVPADRDIRGTLDAPDADRCVVACPPHPQHGGNRNDPRLEAVSDDLDAACLRFDYGPWDEGRGELEDVRAAYAWARERYDAVGLFGYSFGGCLALVAAAAESEAGTPPSAVAVLSPAASLAAGELDAVAAVADIDAPMALVYGERDTMIDATAVADALTDAGGDVASLPADHFFVGQTQRVGAAIAAFFNDGALPDSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVS2</accession>
    <name>A0A1U7EVS2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Response regulator CheY</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cheY1</name>
        <name evidence="3" type="ordered locus">NP_2102A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322770.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVS2"/>
    <dbReference type="STRING" id="348780.NP_2102A"/>
    <dbReference type="EnsemblBacteria" id="CAI49142">
        <property type="protein sequence ID" value="CAI49142"/>
        <property type="gene ID" value="NP_2102A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702604"/>
    <dbReference type="KEGG" id="nph:NP_2102A"/>
    <dbReference type="eggNOG" id="arCOG02391">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_15_2"/>
    <dbReference type="OrthoDB" id="2830at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17542">
        <property type="entry name" value="REC_CheY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053548">
        <property type="entry name" value="CheY_signal_transducer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052048">
        <property type="entry name" value="ST_Response_Regulator"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041367">
        <property type="entry name" value="CheY_Halo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43228">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43228:SF1">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR ARR22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="2"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="118" mass="13023" checksum="A0E9F987CB6A5C93" modified="2017-05-10" version="1">MDVLIADDSEFMRSLLREILEEEYNIVGEAENGVEAIELYQEHSPDLVMMDIVMPIRDGIEATDEITDSDPSAKVIMCTSVGQEQKMKSAIQAGAEGYITKPFQKPSVLDEIENVVGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVV0</accession>
    <name>A0A1U7EVV0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Secreted glycoprotein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2184A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322811.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVV0"/>
    <dbReference type="STRING" id="348780.NP_2184A"/>
    <dbReference type="EnsemblBacteria" id="CAI49183">
        <property type="protein sequence ID" value="CAI49183"/>
        <property type="gene ID" value="NP_2184A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701372"/>
    <dbReference type="KEGG" id="nph:NP_2184A"/>
    <dbReference type="HOGENOM" id="CLU_2191098_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="108" mass="11404" checksum="D19D8AF51F7FD257" modified="2017-05-10" version="1">MVSRRGVLAALAVGASSSLAGCHTGALVEVQDMSCVRSRTEPDTCNVLVANHWVSGEFRVVVQSESPDGTVHGAIQEEIHLRAGESRELTLDGDLGDGTLFRASVERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW03</accession>
    <name>A0A1U7EW03_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">NADH dehydrogenase-like complex subunit H</fullName>
            <ecNumber evidence="6">1.6.5.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">nuoH</name>
        <name evidence="6" type="ordered locus">NP_2298A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.6.5.-" evidence="6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322867.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW03"/>
    <dbReference type="STRING" id="348780.NP_2298A"/>
    <dbReference type="EnsemblBacteria" id="CAI49240">
        <property type="protein sequence ID" value="CAI49240"/>
        <property type="gene ID" value="NP_2298A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702992"/>
    <dbReference type="KEGG" id="nph:NP_2298A"/>
    <dbReference type="eggNOG" id="arCOG01546">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015134_0_1_2"/>
    <dbReference type="OrthoDB" id="15253at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01350">
        <property type="entry name" value="NDH1_NuoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001694">
        <property type="entry name" value="NADH_UbQ_OxRdtase_su1/FPO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11432">
        <property type="entry name" value="NADH DEHYDROGENASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11432:SF3">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00146">
        <property type="entry name" value="NADHdh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="25"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="258"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="295"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="333"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI49240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="352" mass="37288" checksum="1AF788E241E1CA04" modified="2017-05-10" version="1">MLQGAPLPERLAELLGLGPGPGSTALAALLGAAIVGTLMMTMTAVAGPWAKRKVTAAFTDRYAVNRLGPFGIIIIVADAVRLLSKELIVPDGVDRPTWDLAPIVLAFSALLGFAVIPMGSGIHLADPEVGLVYVFAIASIASLGLVMAGYASNNKYSLLGGLRAVAQNLAYEIPLVLIAVSVVIFAGSLQMSEIVAAQAEPLVDLGVATIPQWYAFVNPFAFVLFLIANLMEVGRNPFDIPEAPTEIVAGYQTEYSSVYFVLIYLGEFLHIFLGGAIIATLFLGGPAGPGPEALGIVWFLVKIWAVYLFTQWARSALPRLRIDQLIEVGWKGMLVLAFANLILTAIIVGVLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW48</accession>
    <name>A0A1U7EW48_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">L-aspartate oxidase</fullName>
            <ecNumber evidence="4">1.4.3.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Quinolinate synthase B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">nadB</name>
        <name evidence="12" type="ordered locus">NP_2416A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (oxidase route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.16" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322925.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW48"/>
    <dbReference type="STRING" id="348780.NP_2416A"/>
    <dbReference type="EnsemblBacteria" id="CAI49299">
        <property type="protein sequence ID" value="CAI49299"/>
        <property type="gene ID" value="NP_2416A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702962"/>
    <dbReference type="KEGG" id="nph:NP_2416A"/>
    <dbReference type="eggNOG" id="arCOG00572">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014312_3_0_2"/>
    <dbReference type="OrthoDB" id="305271at2157"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00326"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008734">
        <property type="term" value="F:L-aspartate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.700.10:FF:000002">
        <property type="entry name" value="L-aspartate oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.100">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase flavoprotein-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-dep_OxRdtase_2_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037099">
        <property type="entry name" value="Fum_R/Succ_DH_flav-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015939">
        <property type="entry name" value="Fum_Rdtase/Succ_DH_flav-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005288">
        <property type="entry name" value="NadB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42716">
        <property type="entry name" value="L-ASPARTATE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42716:SF2">
        <property type="entry name" value="L-ASPARTATE OXIDASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02910">
        <property type="entry name" value="Succ_DH_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46977">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0274">FAD</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-dependent oxidoreductase 2 FAD-binding" evidence="10">
        <location>
            <begin position="10"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="Fumarate reductase/succinate dehydrogenase flavoprotein-like C-terminal" evidence="11">
        <location>
            <begin position="419"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI49299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="501" mass="52700" checksum="2CAE1D125CAAA42B" modified="2017-05-10" version="1">MTGEPRETELLVVGSGIAGCAAALAGARAGASVTLATKATRPEESTSYWAQGGIAVSRGDAEQFKRDIIAASDGLADETAVDVLVESADEAVEDVLVETLGVDFDGDDGHEYAREAAHSEPRILHVDAATGKHLLGPFMSHLESHDDVEILADTAALELVTAEGRVHGAMLEQNGEWAPCLAGATVLATGGVGACYGRTTNPPTAAGDGVAMAALAGADTADMEYVQFHPTAFAGEDGDDRGTFLLSEAVRGEGALLRNADGERFMPDYHEDAELAPRDVVARAVRAERERTGGVSLDVGPVDFEAEFPGLASACRDRGVDIEAGIPVAPAEHFLCGGVAVDTEGRTSLDQLYAVGECARTGVHGANRLASTSLLEGLVWGLRAGEAAAGNEPVAVEVPELRDSDPSLPDSFAEEKFIRLRRVMDDYVGIERTPSDLDRAAGVLRRLKGEIDAYIRTRTSRSLYELRNACIAALLITRAARENETSRGCHHLVEEAAADAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW66</accession>
    <name>A0A1U7EW66_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Cox-type terminal oxidase subunit III</fullName>
            <ecNumber evidence="10">1.9.3.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">coxC</name>
        <name evidence="10" type="ordered locus">NP_2450A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the cytochrome c oxidase subunit 3 family.</text>
    </comment>
    <dbReference type="EC" id="1.9.3.-" evidence="10"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322942.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW66"/>
    <dbReference type="STRING" id="348780.NP_2450A"/>
    <dbReference type="EnsemblBacteria" id="CAI49316">
        <property type="protein sequence ID" value="CAI49316"/>
        <property type="gene ID" value="NP_2450A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702628"/>
    <dbReference type="KEGG" id="nph:NP_2450A"/>
    <dbReference type="eggNOG" id="arCOG04650">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044071_0_1_2"/>
    <dbReference type="OrthoDB" id="248633at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019646">
        <property type="term" value="P:aerobic electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00386">
        <property type="entry name" value="Heme_Cu_Oxidase_III_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.80">
        <property type="entry name" value="Cytochrome c oxidase, subunit III, four-helix bundle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024791">
        <property type="entry name" value="Cyt_c/ubiquinol_Oxase_su3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000298">
        <property type="entry name" value="Cyt_c_oxidase-like_su3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035973">
        <property type="entry name" value="Cyt_c_oxidase_su3-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013833">
        <property type="entry name" value="Cyt_c_oxidase_su3_a-hlx"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11403:SF2">
        <property type="entry name" value="CYTOCHROME BO(3) UBIQUINOL OXIDASE SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11403">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00510">
        <property type="entry name" value="COX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81452">
        <property type="entry name" value="Cytochrome c oxidase subunit III-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50253">
        <property type="entry name" value="COX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="107"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="221"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="267"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="Heme-copper oxidase subunit III family profile" evidence="9">
        <location>
            <begin position="21"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI49316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="288" mass="31381" checksum="4770C8A34FCAEF5A" modified="2017-05-10" version="1">MSVETDDGHGHGHGHHLPAVEDWPKGFGEASWWPFIAALGGSGLYIGAALFVLGQPEHGVVSPLAGPLVFVLSTAAFLAGLYGWLYHAFIVDFWAGEADDHGFPMKLTMLLFLGTEVATFGAGFVYYFFIRADAWANLPPIVADGNVLNALVIANTLILIASSVTMHYAHHALLKDNRKRFVRLLGATLLLGLIFLAGQTYEYYEFITVYGFGLTDGAYGSAFYALTGLHGLHVALGAVLLTILFIRAIYGHYSPEKHVSVSTVSMYWHFVDIVWVFLVVVLYVGAIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW68</accession>
    <name>A0A1U7EW68_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2458A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49320.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW68"/>
    <dbReference type="EnsemblBacteria" id="CAI49320">
        <property type="protein sequence ID" value="CAI49320"/>
        <property type="gene ID" value="NP_2458A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_2458A"/>
    <dbReference type="eggNOG" id="arCOG15238">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_220172_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49320.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="36" mass="3960" checksum="9CF7A110BC942C65" modified="2017-05-10" version="1">MAKTREFTEKEYLLVVLVGVALLMATTAFILVITSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW71</accession>
    <name>A0A1U7EW71_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cox cluster protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2454A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322944.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW71"/>
    <dbReference type="STRING" id="348780.NP_2454A"/>
    <dbReference type="EnsemblBacteria" id="CAI49318">
        <property type="protein sequence ID" value="CAI49318"/>
        <property type="gene ID" value="NP_2454A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701448"/>
    <dbReference type="KEGG" id="nph:NP_2454A"/>
    <dbReference type="eggNOG" id="arCOG06261">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149762_0_0_2"/>
    <dbReference type="OrthoDB" id="306958at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046506">
        <property type="entry name" value="DUF6684"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20389">
        <property type="entry name" value="DUF6684"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="41"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="95" mass="10060" checksum="6D77A5454A90DEF6" modified="2017-05-10" version="1">MSNSVFDRETMLDLSVNVIPLAVLLFFIAAFAVIAPFPGNAVVVVVQMSIIVLTGFGLAVLTYYSGKAVAGDETDDQIPPGYSREDAAGPAHKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW84</accession>
    <name>A0A1U7EW84_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 8">Glutamyl-tRNA(Gln) amidotransferase subunit D</fullName>
            <shortName evidence="5">Glu-ADT subunit D</shortName>
            <ecNumber evidence="5 8">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 12" type="primary">gatD</name>
        <name evidence="12" type="ordered locus">NP_2492A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 8">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The GatDE system is specific for glutamate and does not act on aspartate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5 8">Heterodimer of GatD and GatE.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 8">Belongs to the asparaginase 1 family. GatD subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="5 8"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322963.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW84"/>
    <dbReference type="STRING" id="348780.NP_2492A"/>
    <dbReference type="EnsemblBacteria" id="CAI49337">
        <property type="protein sequence ID" value="CAI49337"/>
        <property type="gene ID" value="NP_2492A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702738"/>
    <dbReference type="KEGG" id="nph:NP_2492A"/>
    <dbReference type="eggNOG" id="arCOG01924">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019134_2_1_2"/>
    <dbReference type="OrthoDB" id="371959at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08962">
        <property type="entry name" value="GatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1170">
        <property type="entry name" value="L-asparaginase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00586">
        <property type="entry name" value="GatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006033">
        <property type="entry name" value="AsnA_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036152">
        <property type="entry name" value="Asp/glu_Ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006034">
        <property type="entry name" value="Asparaginase/glutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020827">
        <property type="entry name" value="Asparaginase/glutaminase_AS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027475">
        <property type="entry name" value="Asparaginase/glutaminase_AS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040919">
        <property type="entry name" value="Asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011878">
        <property type="entry name" value="GatD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040918">
        <property type="entry name" value="GatD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037222">
        <property type="entry name" value="GatD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027473">
        <property type="entry name" value="L-asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027474">
        <property type="entry name" value="L-asparaginase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037152">
        <property type="entry name" value="L-asparaginase_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00519">
        <property type="entry name" value="asnASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02153">
        <property type="entry name" value="gatD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003217">
        <property type="entry name" value="PRK04183.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707:SF28">
        <property type="entry name" value="60 KDA LYSOPHOSPHOLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00710">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17763">
        <property type="entry name" value="Asparaginase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18195">
        <property type="entry name" value="GatD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500175">
        <property type="entry name" value="Glu_ADT_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001220">
        <property type="entry name" value="L-ASNase_gatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00139">
        <property type="entry name" value="ASNGLNASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00057">
        <property type="entry name" value="Glutaminase/Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00870">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141300">
        <property type="entry name" value="GatD N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53774">
        <property type="entry name" value="Glutaminase/Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00144">
        <property type="entry name" value="ASN_GLN_ASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00917">
        <property type="entry name" value="ASN_GLN_ASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51732">
        <property type="entry name" value="ASN_GLN_ASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 5 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 5 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GatD N-terminal" evidence="11">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="L-asparaginase N-terminal" evidence="9">
        <location>
            <begin position="79"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Asparaginase/glutaminase C-terminal" evidence="10">
        <location>
            <begin position="290"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 7">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF18195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI49337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="414" mass="44538" checksum="52C50A8D06A9059A" modified="2017-05-10" version="1">MNPGDRVRVDRADVTYEGVLMPSTTDEHLVVKLDGGYNVGIDRADADTEVLETDTYDVAEAQTAEGTSEITFEDDLPTVSLISTGGTIASTVDYRTGAVTAQFDAEDVLRAVPELAGRANYRGRVVANILSENMTPEVWQELAEAVREEIEAGADGVVVMHGTDTMQYTAAALSFMLDTPVPVVFTGSQRSADRPSSDNVMNAVCAVEAATADHSEVLVCMHATESDDNCALHRGTRVRKNHTSRRDAFETVGAEPLGNVDWETGDVSFRRPVADRGDADPRLQGELNTDVELVTFVPGTDADRLAVAEGADGLVIEGTGLGHVNTDWIARIDELTDDGTTVVMTSQCIEGRVCDRVYDTGRDLLDAGVIEAGDTLPATAYVKLMWALEHADSVAEAMQRPLAGELQERSTPWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWA6</accession>
    <name>A0A1U7EWA6_NATPD</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2554A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
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            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49368.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWA6"/>
    <dbReference type="STRING" id="348780.NP_2554A"/>
    <dbReference type="EnsemblBacteria" id="CAI49368">
        <property type="protein sequence ID" value="CAI49368"/>
        <property type="gene ID" value="NP_2554A"/>
    </dbReference>
    <dbReference type="GeneID" id="43500088"/>
    <dbReference type="KEGG" id="nph:NP_2554A"/>
    <dbReference type="HOGENOM" id="CLU_3178674_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49368.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="46" mass="4712" checksum="907FC14705635C05" modified="2017-05-10" version="1">MEADRPSATEANAEEPGAYLTPRADFLIIAGLTLCVAVAVAAVILP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWC4</accession>
    <name>A0A1U7EWC4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2580A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWC4"/>
    <dbReference type="STRING" id="348780.NP_2580A"/>
    <dbReference type="EnsemblBacteria" id="CAI49381">
        <property type="protein sequence ID" value="CAI49381"/>
        <property type="gene ID" value="NP_2580A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701495"/>
    <dbReference type="KEGG" id="nph:NP_2580A"/>
    <dbReference type="eggNOG" id="arCOG06214">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147793_0_0_2"/>
    <dbReference type="OrthoDB" id="191840at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055540">
        <property type="entry name" value="DUF7116"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23429">
        <property type="entry name" value="DUF7116"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="104" mass="11729" checksum="5F4A20742E543270" modified="2017-05-10" version="1">MGAVTTSLDEEARTIFRDLGYEVTTENDELRAERKWRTVHVTTADPEEANTYGRLRCFVADADRAGEVRDRLLGLEPDYDWAVMSVEDGDYQVLHPSADVLPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWH0</accession>
    <name>A0A1U7EWH0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Thioredoxin-disulfide reductase</fullName>
            <ecNumber evidence="7">1.8.1.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">trxB1</name>
        <name evidence="7" type="ordered locus">NP_2680A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.8.1.9" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323056.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWH0"/>
    <dbReference type="STRING" id="348780.NP_2680A"/>
    <dbReference type="EnsemblBacteria" id="CAI49431">
        <property type="protein sequence ID" value="CAI49431"/>
        <property type="gene ID" value="NP_2680A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703427"/>
    <dbReference type="KEGG" id="nph:NP_2680A"/>
    <dbReference type="eggNOG" id="arCOG01296">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031864_5_2_2"/>
    <dbReference type="OrthoDB" id="265365at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050097">
        <property type="entry name" value="Ferredoxin-NADP_redctase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008255">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_2_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48105">
        <property type="entry name" value="THIOREDOXIN REDUCTASE 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00573">
        <property type="entry name" value="PYRIDINE_REDOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="6">
        <location>
            <begin position="9"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="349" mass="36625" checksum="BF696B1AD2877C59" modified="2017-05-10" version="1">MTDDADHRRLIIAGSGIAGLTAAIYAARSNNDPLVLEGPEPGGQLTLTTDVANYPGFPDGIGGPDLVNNMKEQAEQFGATIDHGVIDAVDPGQPHRIELRDGTVYTADALIAASGASARTLGVPGEDELMGYGVSTCATCDGAFFRGEDMLVVGGGDAACEEAAFLTKFADTVYLAHRREEFRAEDYWVDRIRELEADGDVEVLTNTELVEIHGTPEDGVDHVTLAHNPDGYPSEKLDDDATETTERDLGAVFMAIGHTPNTEYLEGTGVEMDATGYIHTQGGEGGGQTKTDVSGIFGAGDVVDHHYQQAITAGGMGCKAAIDADEYLEALEREEGAVADADAAAASDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWH1</accession>
    <name>A0A1U7EWH1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2682A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323057.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWH1"/>
    <dbReference type="STRING" id="348780.NP_2682A"/>
    <dbReference type="EnsemblBacteria" id="CAI49432">
        <property type="protein sequence ID" value="CAI49432"/>
        <property type="gene ID" value="NP_2682A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701523"/>
    <dbReference type="KEGG" id="nph:NP_2682A"/>
    <dbReference type="eggNOG" id="arCOG08172">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1369565_0_0_2"/>
    <dbReference type="OrthoDB" id="242058at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="199" mass="21518" checksum="D7F98FC5D864C7E1" modified="2017-05-10" version="1">MVPGVPDPGVVDRLDIQRDEPDPADRLHADETLLTTVRLSPGWVAVTERALLTFHPDRDPKVVRTPRANVTGITVRRTGGRGFLRYVPGALLYALGAGLVGVVLLTVSPGQFLTVPDAPGAGQLDAIIQTLGWAVRLLGSVLVFTAILAVLTAAVVVLYWLFSRDVAFIIERGDAEPVECPTNRQVGQRALRELRETLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWJ1</accession>
    <name>A0A1U7EWJ1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">isochorismate synthase</fullName>
            <ecNumber evidence="3">5.4.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Isochorismate mutase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">menF</name>
        <name evidence="8" type="ordered locus">NP_2724A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>chorismate = isochorismate</text>
            <dbReference type="Rhea" id="RHEA:18985"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29780"/>
            <dbReference type="EC" id="5.4.4.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the isochorismate synthase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.4.2" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323078.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWJ1"/>
    <dbReference type="STRING" id="348780.NP_2724A"/>
    <dbReference type="EnsemblBacteria" id="CAI49453">
        <property type="protein sequence ID" value="CAI49453"/>
        <property type="gene ID" value="NP_2724A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702912"/>
    <dbReference type="KEGG" id="nph:NP_2724A"/>
    <dbReference type="eggNOG" id="arCOG02015">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006493_8_4_2"/>
    <dbReference type="OrthoDB" id="195185at2157"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00040"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008909">
        <property type="term" value="F:isochorismate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.120.10">
        <property type="entry name" value="Anthranilate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005801">
        <property type="entry name" value="ADC_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015890">
        <property type="entry name" value="Chorismate_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004561">
        <property type="entry name" value="IsoChor_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00543">
        <property type="entry name" value="isochor_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42839">
        <property type="entry name" value="ISOCHORISMATE SYNTHASE ENTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42839:SF2">
        <property type="entry name" value="ISOCHORISMATE SYNTHASE ENTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00425">
        <property type="entry name" value="Chorismate_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56322">
        <property type="entry name" value="ADC synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chorismate-utilising enzyme C-terminal" evidence="7">
        <location>
            <begin position="182"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="156"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="173"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00041564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="445" mass="48092" checksum="C8453BC2073AD5EF" modified="2017-05-10" version="1">MEPLRSTELCPDETQLVSRSRRLSAPPDPRTALSMADAPRTLWAAPDEATVVGSGAAETIRADGNDRFRAVREAANELFATGDVHAGTLSARPRLFGGFAFHDEHDPAGPWRGFPGARFVLPETQLTYAEDGTWLTVHGVGRDVEAVESALEAAADRFDGATTPRPPAGPPGIERRERTTSKDRWRESVTAAIDRIEAGELRKVVLAQALQTALSRPVSLPDVLTRLADTYPDCYRFLIEPDDGGAGFFGATPERLVGRRGRTVETGALAGTTGRGDTPLEDEWLAEELRSDAKNVHEHELVADAIRDQLQPYAASVRTGERGVRRLATVQHLETPITAELAADEHVLSLVEALHPTPAVGGLPPDKALATIRDTEPFERGWYAAPVGWFDAAGNGSFAVAIRSAVAHGEAATLFAGVGIVADSDPDREWDEVQLKYRPILDELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWK1</accession>
    <name>A0A1U7EWK1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Lrp/AsnC family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_2752A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49467.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939551.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWK1"/>
    <dbReference type="STRING" id="348780.NP_2752A"/>
    <dbReference type="EnsemblBacteria" id="CAI49467">
        <property type="protein sequence ID" value="CAI49467"/>
        <property type="gene ID" value="NP_2752A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701546"/>
    <dbReference type="KEGG" id="nph:NP_2752A"/>
    <dbReference type="eggNOG" id="arCOG01580">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_5_4_2"/>
    <dbReference type="OrthoDB" id="199244at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043200">
        <property type="term" value="P:response to amino acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049859">
        <property type="entry name" value="LrpA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041395">
        <property type="entry name" value="TranRegLrpA1_Halo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154">
        <property type="entry name" value="LEUCINE-RESPONSIVE REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154:SF34">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR AZLB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="4"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49467.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="141" mass="15447" checksum="6329641985E1DA0A" modified="2017-05-10" version="1">MNAETEHRILAILEDDAQASYAEIADRADVSKPTVRKYIDKLESEGVIVGYSADIDPKKLSGTSIAMVGIDVASERYVEVTRELKNLDELESLYTSSGDHMLMAEVRAADGDAVGDVIHEKILTIDGVEAAHPSFLQERLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWL6</accession>
    <name>A0A1U7EWL6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Amidohydrolase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2740A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323086.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWL6"/>
    <dbReference type="STRING" id="348780.NP_2740A"/>
    <dbReference type="EnsemblBacteria" id="CAI49461">
        <property type="protein sequence ID" value="CAI49461"/>
        <property type="gene ID" value="NP_2740A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701540"/>
    <dbReference type="KEGG" id="nph:NP_2740A"/>
    <dbReference type="eggNOG" id="arCOG01931">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044590_0_0_2"/>
    <dbReference type="OrthoDB" id="34429at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019748">
        <property type="term" value="P:secondary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01292">
        <property type="entry name" value="metallo-dependent_hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032465">
        <property type="entry name" value="ACMSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21240">
        <property type="entry name" value="2-AMINO-3-CARBOXYLMUCONATE-6-SEMIALDEHYDE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21240:SF28">
        <property type="entry name" value="ISO-OROTATE DECARBOXYLASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04909">
        <property type="entry name" value="Amidohydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="2">
        <location>
            <begin position="8"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="275" mass="29918" checksum="120D0F2F0B6B222D" modified="2017-05-10" version="1">MSFAPATDCHVHLLPRRLTEAIRSALGGEAGWSFQHSPDPTAIEETLLSAGVERYISLPYAHRPGMAADLNDWVLEWAAASEACVPFATVHPEDDVREVVRAAFDGGARGLKFQCPVQECSPDDPRLAPAFELAAERDRSVLFHAGTAPLYEESPYVGVDRFRAFCASYPNVSVCCAHMGTYETDAFLEVLADNEQAFLDTSFAMADSEYVAFDADSIDNSVFETFAGQIMYGSDFPNIPHAYRAERAGLLARDLSSAATDALFRGAADRFLGTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWP3</accession>
    <name>A0A1U7EWP3_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cobalamin import ATP-binding protein BtuD</fullName>
            <ecNumber evidence="8">7.6.2.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Vitamin B12-transporting ATPase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">znuC1</name>
        <name evidence="12" type="synonym">abc19a</name>
        <name evidence="12" type="ordered locus">NP_2864A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for corrinoid utilization. Probably part of the ABC transporter complex BtuCDF involved in cobalamin (vitamin B12) import. Probably responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>an R-cob(III)alamin(out) + ATP + H2O = an R-cob(III)alamin(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17873"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:140785"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.6.2.8"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">The complex is composed of two ATP-binding proteins (BtuD), two transmembrane proteins (BtuC) and a solute-binding protein (BtuF).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.8" evidence="8"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323148.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWP3"/>
    <dbReference type="STRING" id="348780.NP_2864A"/>
    <dbReference type="EnsemblBacteria" id="CAI49523">
        <property type="protein sequence ID" value="CAI49523"/>
        <property type="gene ID" value="NP_2864A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702423"/>
    <dbReference type="KEGG" id="nph:NP_2864A"/>
    <dbReference type="eggNOG" id="arCOG00201">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_11_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015420">
        <property type="term" value="F:ABC-type vitamin B12 transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03235">
        <property type="entry name" value="ABC_Metallic_Cations"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000134">
        <property type="entry name" value="Iron-enterobactin ABC transporter ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050153">
        <property type="entry name" value="Metal_Ion_Import_ABC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42734:SF5">
        <property type="entry name" value="IRON TRANSPORT SYSTEM ATP-BINDING PROTEIN HI_0361-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42734">
        <property type="entry name" value="METAL TRANSPORT SYSTEM ATP-BINDING PROTEIN TM_0124-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="5"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00050590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00064420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00077139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI49523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="247" mass="27128" checksum="77F19CA02E48AA15" modified="2017-05-10" version="1">MSTVVDVQNVTFAYGETPAVEDVSLSISDGEFVGLIGPNGSGKTTLLHLMIGLYEPDSGSIELFGEPATEFDEGERLGYVGQRSTDRSGTMPVTVREAILMGRFARAGRSRLTDEDRRIVADAMETVDIRELANRRVDELSGGQRQRAFIARALASEADILALDEPTVGVDADSRDRFYDLLDELNRNGMTIILIEHDIDILTKHVDTVACINRELYHHGDTVSFLESDALSEAYGRTSGIIQHNHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWR5</accession>
    <name>A0A1U7EWR5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable secreted glycoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2908A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323170.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWR5"/>
    <dbReference type="STRING" id="348780.NP_2908A"/>
    <dbReference type="EnsemblBacteria" id="CAI49545">
        <property type="protein sequence ID" value="CAI49545"/>
        <property type="gene ID" value="NP_2908A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701593"/>
    <dbReference type="KEGG" id="nph:NP_2908A"/>
    <dbReference type="eggNOG" id="arCOG02916">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_741064_0_0_2"/>
    <dbReference type="OrthoDB" id="121941at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008964">
        <property type="entry name" value="Invasin/intimin_cell_adhesion"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49373">
        <property type="entry name" value="Invasin/intimin cell-adhesion fragments"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="373" mass="40098" checksum="621CBB67937C866B" modified="2017-05-10" version="1">MSLTTDTRGVSEVVGAILLFGLLVAVLAILQTQAIPTANEEIEFNHNQEVQNDLIEFQEAASRTAAHGTTESVGIRAGTTYPSRLLFFNPPNPAGTVRTVEDGEVTIENVEATDDIIRDAHIDGEIDELETSRIEYEPIYNEYQNPPVTALEYGILYNSFPDAQVVENTGAVVSGNNINLMFYAGDVSQATSGSITLDTIPASAPSRTVTVEPTDDIEITVPSNLDATEWEETVFEDEDAVTVSDSGDDIVIEIDEDAHENFELRMSQVGVGSAVASADAEYIYPTETGNAVTVDEDETVEIPIEVRDRYNNPVSGVELEYEANEGDASGPAATDANGQAIVTYEASDEDDTITIDAPDAGAVDEFDIDVTVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWS2</accession>
    <name>A0A1U7EWS2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Type IV pilus biogenesis complex membrane subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">NP_2910A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323171.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWS2"/>
    <dbReference type="STRING" id="348780.NP_2910A"/>
    <dbReference type="EnsemblBacteria" id="CAI49546">
        <property type="protein sequence ID" value="CAI49546"/>
        <property type="gene ID" value="NP_2910A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701594"/>
    <dbReference type="KEGG" id="nph:NP_2910A"/>
    <dbReference type="eggNOG" id="arCOG01808">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017646_1_1_2"/>
    <dbReference type="OrthoDB" id="12374at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.81.30">
        <property type="entry name" value="Type II secretion system (T2SS), domain F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056569">
        <property type="entry name" value="ArlJ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018076">
        <property type="entry name" value="T2SS_GspF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042094">
        <property type="entry name" value="T2SS_GspF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35402">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35402:SF1">
        <property type="entry name" value="TYPE II SECRETION SYSTEM PROTEIN GSPF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00482">
        <property type="entry name" value="T2SSF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="76"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="117"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="274"/>
            <end position="296"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="302"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="387"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="422"/>
            <end position="444"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="586"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="654"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="domain" description="Type II secretion system protein GspF" evidence="7">
        <location>
            <begin position="163"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Type II secretion system protein GspF" evidence="7">
        <location>
            <begin position="463"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="672" mass="75086" checksum="D1CFF7C0C29A84C6" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWW2</accession>
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        <submittedName>
            <fullName evidence="10">Acyl-CoA dehydrogenase</fullName>
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        <name evidence="10" type="primary">acd6</name>
        <name evidence="10" type="ordered locus">NP_3004A</name>
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        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
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            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
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    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
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            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
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    <comment type="similarity">
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    <dbReference type="EnsemblBacteria" id="CAI49593">
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    <dbReference type="KEGG" id="nph:NP_3004A"/>
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        <property type="entry name" value="Acyl-CoA dehydrogenase FadE25"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="3 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 6 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="6"/>
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        </location>
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            <begin position="122"/>
            <end position="218"/>
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            <end position="379"/>
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    </feature>
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        <source>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI49593.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="384" mass="42106" checksum="8AED6D3C4768E186" modified="2017-05-10" version="1">MDFELSDEQQQLKKTVQDFAEEEIIPVAKEYDREEKYPWEVVEKAAENGLLAPQIPLDYGGAGYEVLDTAIIVEELFAADPGIGLCLCSTGFGTEAIIEYGTEEQKEEYLAPIATGDAICGAAISEPDTGSDVSSVSTRAEKDGDEWVINGNKMWITNGSVGDFFVVLCKTDPDAEGRYNGFSQIIVESDRDGFEADKITGKLGIRASDTAELILDDVRVPEENLVGTEGMGFLQQMQFFDETRTGVAAQGVGIAKGAAERALEYAQEREQFGRPIGDFQAIQHKLADMHTNVEAARNLTYKSAWSVDNADGQLTKLASMAKEYASRVAVENANECVQIHGGSGYVDDFDAERFFRDAKITQIYEGTTEIQKMIIARELQGKGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWZ3</accession>
    <name>A0A1U7EWZ3_NATPD</name>
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        <recommendedName>
            <fullName evidence="5">ORC1-type DNA replication protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="8" type="primary">orc3</name>
        <name evidence="8" type="synonym">cdc6C</name>
        <name evidence="8" type="ordered locus">NP_3096A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in regulation of DNA replication.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the CDC6/cdc18 family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
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    <dbReference type="EnsemblBacteria" id="CAI49639">
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        <property type="gene ID" value="NP_3096A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702592"/>
    <dbReference type="KEGG" id="nph:NP_3096A"/>
    <dbReference type="eggNOG" id="arCOG00467">
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    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
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        <property type="entry name" value="AAA_16"/>
    </dbReference>
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        <property type="entry name" value="Cdc6_C"/>
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        <property type="entry name" value="Cdc6_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050311">
        <property type="entry name" value="ORC1/CDC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014277">
        <property type="entry name" value="Orc1/Cdc6_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02928">
        <property type="entry name" value="orc1/cdc6 family replication initiation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763:SF22">
        <property type="entry name" value="ORC1-TYPE DNA REPLICATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22703">
        <property type="entry name" value="Cdc6_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09079">
        <property type="entry name" value="WHD_Cdc6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01074">
        <property type="entry name" value="Cdc6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="51"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Cdc6 C-terminal" evidence="7">
        <location>
            <begin position="307"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="63"/>
            <end position="67"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="398" mass="44393" checksum="615ED8AE82DDB39D" modified="2017-05-10" version="1">MTEDLFSEMTETLFEDKSILSEEYRPDVIVERDDEIDAYRTALKDVLFGRNPSNVFIYGKTGVGKTAVTEYIMNALQTEVSRRDAADELHVHFRNCNGDTVYRTVRALVNSIRGHNREKFPETGLSTSHALETLYEEMDSLGGTFLFVLDEIDHLSDPNTLLYELPRARSNGHLENARVGVIGISNNYTFRSSLSPKVKDTLMEKEIAFSPYDAGELQSILTARAEKALVDGACEESAIRLAAAKAAKDTGSARQAIDLLREGGDVAEEHGAEAITDSHIEVASERVQRGRVQNKLRDQTMHGQLILEAIARLEADGEAPVRSKEVQDSYERVAGRWSRDPLTTLKSIQNHLADLTMLGFLERTEQNEGRAGGVHYQYELTLDPEIVLETRASIETDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX28</accession>
    <name>A0A1U7EX28_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ARM/HEAT repeat protein / protein kinase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">NP_3158A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323292.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX28"/>
    <dbReference type="STRING" id="348780.NP_3158A"/>
    <dbReference type="EnsemblBacteria" id="CAI49670">
        <property type="protein sequence ID" value="CAI49670"/>
        <property type="gene ID" value="NP_3158A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701674"/>
    <dbReference type="KEGG" id="nph:NP_3158A"/>
    <dbReference type="eggNOG" id="arCOG03682">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_540383_0_0_2"/>
    <dbReference type="OrthoDB" id="41005at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004155">
        <property type="entry name" value="PBS_lyase_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43289">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 20-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43289:SF6">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEKL-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13646">
        <property type="entry name" value="HEAT_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00567">
        <property type="entry name" value="EZ_HEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="293"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="263"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="558" mass="60270" checksum="57AEEE9B9D05BF6D" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX60</accession>
    <name>A0A1U7EX60_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HEAT-PBS family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3228A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323327.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX60"/>
    <dbReference type="STRING" id="348780.NP_3228A"/>
    <dbReference type="EnsemblBacteria" id="CAI49705">
        <property type="protein sequence ID" value="CAI49705"/>
        <property type="gene ID" value="NP_3228A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701692"/>
    <dbReference type="KEGG" id="nph:NP_3228A"/>
    <dbReference type="eggNOG" id="arCOG04653">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_661582_0_0_2"/>
    <dbReference type="OrthoDB" id="293146at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004155">
        <property type="entry name" value="PBS_lyase_HEAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12697:SF5">
        <property type="entry name" value="DEOXYHYPUSINE HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12697">
        <property type="entry name" value="PBS LYASE HEAT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13646">
        <property type="entry name" value="HEAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03130">
        <property type="entry name" value="HEAT_PBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00567">
        <property type="entry name" value="EZ_HEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="429" mass="46523" checksum="B0A6336B9CE6EF94" modified="2017-05-10" version="1">MSNGDDESTEDAPDEAASDGEDPDISIEEFETRLDDIEAALEDASTEDDLDAVEADADDVEADLETVEFPVPEPDDEDEEPDDPKEPLVDRLSDIRDGIEDQRGPYGDDVASDANGAAGQIRSTRWTRDGRPAVKAAVETFLDAAGEQLDTDFEAGDDIDAFADAVETVAETAADGRLDADDDAETIAALLDAVDDLESDLDDAEEWSDLEVAEQLRREGFYDRLTAENRRDFPPELNVVRIAEAENDPERILMAFEQLDSDFMQENCIDALSRLGPEAAFEEMNQLAQRREVGAIEVLGKIGDERAVETLSDFVDSGNPPLQKATIRALGEIGSQEATQPLANALADEEPMVRSMAARGLGLIGDTRAIAPLEDVLGDDDEPPEVRASAAWALVQIGTERALEAAAAYDDDRVYTVQLEAEKAREATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX74</accession>
    <name>A0A1U7EX74_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CRISPR-associated DNA-binding protein Csm6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">csm6</name>
        <name evidence="3" type="synonym">csa3</name>
        <name evidence="3" type="ordered locus">NP_3260A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323342.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX74"/>
    <dbReference type="STRING" id="348780.NP_3260A"/>
    <dbReference type="EnsemblBacteria" id="CAI49721">
        <property type="protein sequence ID" value="CAI49721"/>
        <property type="gene ID" value="NP_3260A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701705"/>
    <dbReference type="KEGG" id="nph:NP_3260A"/>
    <dbReference type="eggNOG" id="arCOG01446">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107894_0_0_2"/>
    <dbReference type="OrthoDB" id="116640at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054588">
        <property type="entry name" value="Csa3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22662">
        <property type="entry name" value="Csa3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Csa3 N-terminal" evidence="2">
        <location>
            <begin position="2"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="146"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="215" mass="23817" checksum="09099D5E72165289" modified="2017-05-10" version="1">MRTYISPIGYDTRRATRPVINQGMQGGDMLVLLRPEAESDTEWANQTIADVKQYLHEAEPAFEINVERVETASFEATVRDCCRLLGDIEPDRETIVSLGGGARDVLLPLTVASLVYANQIDTALFFSDLDSTVREWVLPDLTTQIPDRTTDTFTIILEAGGWISLSEIVSRTSHSKSTVVRHVNDLEEAGVIQADTSEKAKRVRVTFSGEILSMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXC7</accession>
    <name>A0A1U7EXC7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3484A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323453.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXC7"/>
    <dbReference type="STRING" id="348780.NP_3484A"/>
    <dbReference type="EnsemblBacteria" id="CAI49833">
        <property type="protein sequence ID" value="CAI49833"/>
        <property type="gene ID" value="NP_3484A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701785"/>
    <dbReference type="KEGG" id="nph:NP_3484A"/>
    <dbReference type="eggNOG" id="arCOG06407">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_901992_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="320" mass="33478" checksum="F27D1610D84C788D" modified="2017-05-10" version="1">MPSRRRLLRNAAALTLGGLVASAPATAQSPDSPSEYPRYDAGAVADTETVVAATCDPAVVSETPLPAAVRNRLAALRRRHDAFALSDTRRLTGSVAVDGTAVEGCVTLAGAFDADAADAELRADDTLRRHPDEGGLHRFDAPDVSVSLAFDDQLLRVGYAPDLGRATDHLEAAISGSESATNDAHGALPALLGGDAAVYARLGDRERQRAIDALGDKAPDELGAVLSRAKAVGLSLQAVPEQSRIRYGAAGARLDTDTVRRLAADAQQREGALSDVRLHRQGRTIIADAGVETAVAWNAHARLVGRESDAEARGLTMSDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXJ2</accession>
    <name>A0A1U7EXJ2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DUF2892 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3574A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323498.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXJ2"/>
    <dbReference type="STRING" id="348780.NP_3574A"/>
    <dbReference type="EnsemblBacteria" id="CAI49878">
        <property type="protein sequence ID" value="CAI49878"/>
        <property type="gene ID" value="NP_3574A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701812"/>
    <dbReference type="KEGG" id="nph:NP_3574A"/>
    <dbReference type="eggNOG" id="arCOG07779">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_176022_2_1_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021309">
        <property type="entry name" value="YgaP-like_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11127">
        <property type="entry name" value="YgaP-like_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="Inner membrane protein YgaP-like transmembrane" evidence="2">
        <location>
            <begin position="4"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="69" mass="7089" checksum="E17EA9144A2BC32B" modified="2017-05-10" version="1">MSTETNVGGRDRLFRAALAVVLSVVALRLFTAGKRKTASVAVVGAVGFGFNAVACFCGLNELLGIDTAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXJ4</accession>
    <name>A0A1U7EXJ4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3656A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323537.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXJ4"/>
    <dbReference type="EnsemblBacteria" id="CAI49919">
        <property type="protein sequence ID" value="CAI49919"/>
        <property type="gene ID" value="NP_3656A"/>
    </dbReference>
    <dbReference type="GeneID" id="54763323"/>
    <dbReference type="KEGG" id="nph:NP_3656A"/>
    <dbReference type="eggNOG" id="arCOG06242">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_212427_0_0_2"/>
    <dbReference type="OrthoDB" id="204433at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="46" mass="5067" checksum="67891CD5FEFC3C5C" modified="2017-05-10" version="1">MTAASDRESAPIELRAHSTSPEKTVFTEPGNSDGWIATDYTVDVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXJ5</accession>
    <name>A0A1U7EXJ5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3622A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXJ5"/>
    <dbReference type="EnsemblBacteria" id="CAI49902">
        <property type="protein sequence ID" value="CAI49902"/>
        <property type="gene ID" value="NP_3622A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3622A"/>
    <dbReference type="eggNOG" id="arCOG10183">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1375561_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="198" mass="23250" checksum="7F5FA7206CEE5E1A" modified="2017-05-10" version="1">MRKADTLLRRFRDACSARRWAVYSLGFLPVVWLAVRLLPLWRIRRWWAVRLVRLPDRWLPTHLRVLPPPDSEYLGVWEVPPDEARSRLETRYGFRQQVRAYLHAYERHGTIRYEQASCAYRPNGLLGGWQLHVRLFSAAGGTAVWCHWERNPNVAPLAHLRRDGYDPDAGKQRLLELLDEPLRVADESGHAPPYPDRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXT2</accession>
    <name>A0A1U7EXT2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3832A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323624.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXT2"/>
    <dbReference type="STRING" id="348780.NP_3832A"/>
    <dbReference type="EnsemblBacteria" id="CAI50007">
        <property type="protein sequence ID" value="CAI50007"/>
        <property type="gene ID" value="NP_3832A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701877"/>
    <dbReference type="KEGG" id="nph:NP_3832A"/>
    <dbReference type="eggNOG" id="arCOG06323">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066582_0_0_2"/>
    <dbReference type="OrthoDB" id="312988at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055538">
        <property type="entry name" value="DUF7114"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23426">
        <property type="entry name" value="DUF7114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="221" mass="23438" checksum="5B097EA81581D81B" modified="2017-05-10" version="1">MDETAHARAAFREGLADITPGALRDHLEEVLEDASLTPGALTVATATALDGAVDRDLAARRGAGVQMSYEGLRLSRSLIRTTPWEHGDRESGDLDVIAAEVLVARGFEYLAHTGVALDVVEAVQQFGRTQTLREAPDCEAVPATRLEADFVRIAVEAGADVALESTPQEVTALAEALAEEFSADPLPTPDVVLADADRRLERLALSHDRPTAGDVSGSSET</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXU1</accession>
    <name>A0A1U7EXU1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3856A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323636.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXU1"/>
    <dbReference type="STRING" id="348780.NP_3856A"/>
    <dbReference type="EnsemblBacteria" id="CAI50019">
        <property type="protein sequence ID" value="CAI50019"/>
        <property type="gene ID" value="NP_3856A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701889"/>
    <dbReference type="KEGG" id="nph:NP_3856A"/>
    <dbReference type="eggNOG" id="arCOG06948">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_820440_0_0_2"/>
    <dbReference type="OrthoDB" id="205297at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="289"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="351" mass="39812" checksum="6B5FA95334F44A55" modified="2017-05-10" version="1">MNLARQHLTLLLNAYYELDEQTITVEHPASELGELLRIDLSDPAASTVSITTGAVSYEQHLEEIEREYGSQVREELPNRAAYVKALVASGLVDIRNREEVDKIIDRYGYQDLQAGHPPRYAGFDTNLFPWRMHDVLGIDPELYSDDTGRTPVNGYTLPAGVDEELSISYRYDKNAMDAERLADALGPEFERLAGQPTEDNRETRLGLREYRRLRETRSHDIIASEPSDEAIIEGCLEYYENEPTGVILFSNDYGFVDQARDRKIPAVHVDFAIDVQRQLTGSWDQIATLLYVFAVTFGVIVLPKATLYGVWQAKASQNWRQEEIHVDARSGKLEALLERDKPIVDAHIASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXY4</accession>
    <name>A0A1U7EXY4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DNA replication factor GINS</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ginS</name>
        <name evidence="3" type="ordered locus">NP_3992A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323703.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXY4"/>
    <dbReference type="STRING" id="348780.NP_3992A"/>
    <dbReference type="EnsemblBacteria" id="CAI50087">
        <property type="protein sequence ID" value="CAI50087"/>
        <property type="gene ID" value="NP_3992A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701931"/>
    <dbReference type="KEGG" id="nph:NP_3992A"/>
    <dbReference type="eggNOG" id="arCOG00551">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047666_0_0_2"/>
    <dbReference type="OrthoDB" id="157576at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd11714">
        <property type="entry name" value="GINS_A_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054314">
        <property type="entry name" value="Gins51_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22090">
        <property type="entry name" value="Gins51_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gins51 C-terminal" evidence="2">
        <location>
            <begin position="303"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="243"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="269"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="349" mass="36516" checksum="078682D14C64FC01" modified="2017-05-10" version="1">MNLDELQSVQARERQSSDLQQLRPSFYREVGAFVQELTEQRARVVEEVDDPFSSPKVRQLTNDIETAEQTVEAIYERRVGKVVKKASIAAAGMPVDDAGLTDEEERLFEDLVDRIEDNRDAVLSVLDGDGPDVSCSVGGTEQPKSKTEPAAADTSEPDAPTAPDGRSSPSAEDGVSAADLMGSGSDEGGDSDEPAADTPVEPSAPDQSTPSEAEQPTASDPEQPAPPEPEQPSTATAEQPIPSEGPPAPNPEQPSGDGHPVPPDPPEQAEATDGGTAAAGPSTESASDATETASSMPGLPRTTVRITADVGEIVGADDRDYELGSEDVVTLPEPNAEVLVEKDAAERLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY19</accession>
    <name>A0A1U7EY19_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lrp/AsnC family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4026A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323720.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY19"/>
    <dbReference type="STRING" id="348780.NP_4026A"/>
    <dbReference type="EnsemblBacteria" id="CAI50104">
        <property type="protein sequence ID" value="CAI50104"/>
        <property type="gene ID" value="NP_4026A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701945"/>
    <dbReference type="KEGG" id="nph:NP_4026A"/>
    <dbReference type="eggNOG" id="arCOG01580">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_1_0_2"/>
    <dbReference type="OrthoDB" id="131500at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043200">
        <property type="term" value="P:response to amino acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154">
        <property type="entry name" value="LEUCINE-RESPONSIVE REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154:SF34">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR AZLB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator AsnC/Lrp ligand binding" evidence="1">
        <location>
            <begin position="70"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="162" mass="18378" checksum="A5ED4F2BBC1016AC" modified="2017-05-10" version="1">MDRADELLELLCENARYSTADLARLTDSDEDEVEATIEELEAEGIIQGYTAVVDWDAAGDERVRAHVELNVTLDRETSYRDVAERLAGFSEIQDLRLVSGDYDFALEVEGENMREVSKFISDKVAPVPEITQTVTHYIMENYKQGGYRFDDDTDDDRLSVTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY27</accession>
    <name>A0A1U7EY27_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Type IV pilus biogenesis complex membrane subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">NP_4020A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323717.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY27"/>
    <dbReference type="STRING" id="348780.NP_4020A"/>
    <dbReference type="EnsemblBacteria" id="CAI50101">
        <property type="protein sequence ID" value="CAI50101"/>
        <property type="gene ID" value="NP_4020A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701943"/>
    <dbReference type="KEGG" id="nph:NP_4020A"/>
    <dbReference type="eggNOG" id="arCOG01808">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017646_1_0_2"/>
    <dbReference type="OrthoDB" id="200343at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.81.30">
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        <property type="entry name" value="TYPE II SECRETION SYSTEM PROTEIN GSPF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00482">
        <property type="entry name" value="T2SSF"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <location>
            <begin position="66"/>
            <end position="90"/>
        </location>
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        <location>
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            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="319"/>
        </location>
    </feature>
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        <location>
            <begin position="386"/>
            <end position="411"/>
        </location>
    </feature>
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        <location>
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            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="585"/>
        </location>
    </feature>
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        </location>
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        <location>
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            <end position="670"/>
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            <end position="282"/>
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        <location>
            <begin position="461"/>
            <end position="584"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00482"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI50101.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="671" mass="73743" checksum="47F79EE05C5223E3" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY30</accession>
    <name>A0A1U7EY30_NATPD</name>
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            <fullName evidence="4">Phosphoserine phosphatase</fullName>
        </recommendedName>
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        <name evidence="2" type="ordered locus">NP_4058A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <dbReference type="NCBI Taxonomy" id="348780"/>
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            <taxon>Archaea</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
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    <dbReference type="STRING" id="348780.NP_4058A"/>
    <dbReference type="EnsemblBacteria" id="CAI50120">
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        <property type="gene ID" value="NP_4058A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701953"/>
    <dbReference type="KEGG" id="nph:NP_4058A"/>
    <dbReference type="eggNOG" id="arCOG01159">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063607_0_0_2"/>
    <dbReference type="OrthoDB" id="147108at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="entry name" value="DUF7121"/>
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    </dbReference>
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            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="187"/>
        </location>
    </feature>
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            <begin position="243"/>
            <end position="283"/>
        </location>
    </feature>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
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            <end position="162"/>
        </location>
    </feature>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
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            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI50120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="296" mass="35091" checksum="95CCBB7B4E11C0E1" modified="2017-05-10" version="1">MAESIDETDNVELTEDDLENKSKGQLIKLAGQLRDRRNELNQMASERASERDDLNAKTREKVDEAQEHREKRDELNEQVQEHKEKRNELNAKANELFDKVDDLKADLELDEGKSIDQLEEEIEDLEFKQQTEVLSSEDERELIEKIEEKREKLSERKEKLDQGGELEELKEEAQEVRAEASQHHQKVTELADKAQEHHNEMIEAYREADDIRDEADEWHDKFVEAQEAADRHHEDFVRVQKRLRELDKEEEEEREEDRAEEREAAKEEAEEIYQKFKEGETLDTEDLMKLQKAGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY58</accession>
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            <fullName evidence="2">Probable S-adenosylmethionine-dependent methyltransferase</fullName>
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        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">NP_4086A</name>
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        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
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            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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    </dbReference>
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    <dbReference type="STRING" id="348780.NP_4086A"/>
    <dbReference type="EnsemblBacteria" id="CAI50134">
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        <property type="gene ID" value="NP_4086A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701966"/>
    <dbReference type="KEGG" id="nph:NP_4086A"/>
    <dbReference type="eggNOG" id="arCOG01782">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060397_2_1_2"/>
    <dbReference type="OrthoDB" id="8915at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050508">
        <property type="entry name" value="Methyltransf_Superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42912:SF45">
        <property type="entry name" value="23S RRNA (GUANINE(745)-N(1))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42912">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="1">
        <location>
            <begin position="45"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="198" mass="21403" checksum="5C0DC38A1EF083E4" modified="2017-05-10" version="1">MDRNDVRRAWDAVAETYADQRDPDGSDAALIDDLLAALPTDPDVLDVGCGDGARTLANLPPGSVGLDISRQGLSLASETVPHALLVYGDMTALPFAADAFDGITAYHAVFHVPRKQHSHVYTEFARVLRPGGRLLMTLPSGRFETVRRDWMGGRMFFSAPGRDRTLAALRSAGFSDLRTETATDPLGSSTEFVFAQHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY68</accession>
    <name>A0A1U7EY68_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_4102A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50142.1"/>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
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    <dbReference type="STRING" id="348780.NP_4102A"/>
    <dbReference type="EnsemblBacteria" id="CAI50142">
        <property type="protein sequence ID" value="CAI50142"/>
        <property type="gene ID" value="NP_4102A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701972"/>
    <dbReference type="KEGG" id="nph:NP_4102A"/>
    <dbReference type="eggNOG" id="arCOG02874">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094837_0_0_2"/>
    <dbReference type="OrthoDB" id="312630at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043826">
        <property type="entry name" value="DUF5803"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19119">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="220"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="257"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="274" mass="30494" checksum="C8A8DC325F471093" modified="2017-05-10" version="1">MNRRLLAAVAVFVLFGLAGCTTIFGADVDDPEALSEEAEYNFDTDRDAFITVNRDNYTAVYNTSASATGDEGTMELWRTNRLTIEQPLELYGLQFRYDNGTTIRYIEGEPMRIHQDGTTEPTDSLSVDNTRRRTIVDLPSADGQLAYTTPKTGKRIAVYAPVHGSYEIALPPDTDAALPLLSQVRPSNDDREEVGDRVHLQWDEVDTSVVVVRWYLDRDLWLFGGLVVIAVIAAVGGAVYYYRQIKQAEKTRKEEGFDIDIDDDGDDGPPPGMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY70</accession>
    <name>A0A1U7EY70_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF420 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4148A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323781.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY70"/>
    <dbReference type="STRING" id="348780.NP_4148A"/>
    <dbReference type="EnsemblBacteria" id="CAI50165">
        <property type="protein sequence ID" value="CAI50165"/>
        <property type="gene ID" value="NP_4148A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701985"/>
    <dbReference type="KEGG" id="nph:NP_4148A"/>
    <dbReference type="eggNOG" id="arCOG02864">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104065_1_0_2"/>
    <dbReference type="OrthoDB" id="202206at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007352">
        <property type="entry name" value="DUF420"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37692:SF1">
        <property type="entry name" value="DUF420 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37692">
        <property type="entry name" value="HYPOTHETICAL MEMBRANE SPANNING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04238">
        <property type="entry name" value="DUF420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="187" mass="20515" checksum="DE685855C6809A93" modified="2017-05-10" version="1">MKLRAWTREHVLGITVVLSVVSLTLVFGAALQAVPTDPLPKPQPLLDAIPHANAVISLAAIGTILGGVYEIRRGNVDRHRLLMVASFLLFAGFLVLYLYRVAIVGPSAFPGGETVRTFVYLPFLAVHIVLAVACVPFVFYALLVAGTRPVEEIYRTNHRTAGRIAAALWLISFSMGIVIYLMLYHIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY84</accession>
    <name>A0A1U7EY84_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Probable methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_4152A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323783.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY84"/>
    <dbReference type="STRING" id="348780.NP_4152A"/>
    <dbReference type="EnsemblBacteria" id="CAI50167">
        <property type="protein sequence ID" value="CAI50167"/>
        <property type="gene ID" value="NP_4152A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701986"/>
    <dbReference type="KEGG" id="nph:NP_4152A"/>
    <dbReference type="eggNOG" id="arCOG00979">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067676_4_0_2"/>
    <dbReference type="OrthoDB" id="21414at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002935">
        <property type="entry name" value="SAM_O-MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43167">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_6G01830)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43167:SF1">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_6G01830)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01596">
        <property type="entry name" value="Methyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51682">
        <property type="entry name" value="SAM_OMT_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="220" mass="23533" checksum="2DC7AD6E706D605E" modified="2017-05-10" version="1">MTVPDDLGAFAAAIGPEPDAVLEEMDARADETGFPTVGPAVGGWLQQLAGLVEARRVFEFGSGFGYSAYWFARALSEDGEVVLTEIDAEELDDARAYFERGGVADRATFEVGDAIEIVRKYEGPFDVVLIDNEKRRYVEAFEAVAPKVPPGGVVVADNMTAGPFEFDEIADLVIGNRTDDGGPAGGIAAYLDHLDDASSFETTLLPLGEGLAVSRRIQRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYL5</accession>
    <name>A0A1U7EYL5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF457 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4462A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323937.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYL5"/>
    <dbReference type="STRING" id="348780.NP_4462A"/>
    <dbReference type="EnsemblBacteria" id="CAI50322">
        <property type="protein sequence ID" value="CAI50322"/>
        <property type="gene ID" value="NP_4462A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702069"/>
    <dbReference type="KEGG" id="nph:NP_4462A"/>
    <dbReference type="eggNOG" id="arCOG03392">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089194_0_0_2"/>
    <dbReference type="OrthoDB" id="200338at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007404">
        <property type="entry name" value="YdjM-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04307">
        <property type="entry name" value="YdjM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="174" mass="18362" checksum="67BB6E66423531E5" modified="2017-05-10" version="1">MQPIVHPVVGYLCYAGYVRASDGEAPQGELTVVAVFAAVLPDLIDQPLWLIGVTPVGRTVAHSLFGGALLVGAVGLLARRRGRTDIGVAFAIGYASHIAADIPWHVLAGDYHELGFLLWPVTGMPAYSGVKSLGTVGAVDITTLWLEAILFVAGAALWWNDGCPGVEVVRRYVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYU1</accession>
    <name>A0A1U7EYU1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">AAA-type ATPase (MoxR subfamily)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_4650A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324030.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYU1"/>
    <dbReference type="STRING" id="348780.NP_4650A"/>
    <dbReference type="EnsemblBacteria" id="CAI50416">
        <property type="protein sequence ID" value="CAI50416"/>
        <property type="gene ID" value="NP_4650A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702134"/>
    <dbReference type="KEGG" id="nph:NP_4650A"/>
    <dbReference type="eggNOG" id="arCOG00434">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034716_2_0_2"/>
    <dbReference type="OrthoDB" id="24581at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000640">
        <property type="entry name" value="MoxR family ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.80">
        <property type="entry name" value="Magnesium chelatase subunit I, C-Terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011703">
        <property type="entry name" value="ATPase_AAA-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050764">
        <property type="entry name" value="CbbQ/NirQ/NorQ/GpvN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041628">
        <property type="entry name" value="ChlI/MoxR_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42759:SF1">
        <property type="entry name" value="MAGNESIUM-CHELATASE SUBUNIT CHLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42759">
        <property type="entry name" value="MOXR FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07726">
        <property type="entry name" value="AAA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17863">
        <property type="entry name" value="AAA_lid_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002849">
        <property type="entry name" value="AAA_ATPase_chaperone_MoxR_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase AAA-3" evidence="3">
        <location>
            <begin position="45"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="ChlI/MoxR AAA lid" evidence="4">
        <location>
            <begin position="238"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI50416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="322" mass="35679" checksum="8E45B8CFDD230759" modified="2017-05-10" version="1">MGVEGPSFAETSNTCKKIIERVSDAVITDREFLKTVLTATLARGHVLLEDVPGTGKTLTALTFAQALGLEFSRIQFTPDLLPNDITGSHVFNEQTGDFEFTKGPVFANVVLADEINRAPPKTQAALLEAMDESQVTVDGTTRRLPDPFLVIATQNPVEQEGTFELPEAQRDRFIVKSSIGYPDKEGELELLERRSQRHAKMPTVDSVLDTEGAQRIQMVPERITMDSAIREYLVTLARETRTDRRVDVGISPRGIQRYFEAARAKAAIEGRDYVAPDDVKQIAEPVMQHRIVLTTDAQIDGISGADVVYDVLERVETPTPQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYV9</accession>
    <name>A0A1U7EYV9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4700A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324055.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYV9"/>
    <dbReference type="EnsemblBacteria" id="CAI50441">
        <property type="protein sequence ID" value="CAI50441"/>
        <property type="gene ID" value="NP_4700A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702149"/>
    <dbReference type="KEGG" id="nph:NP_4700A"/>
    <dbReference type="HOGENOM" id="CLU_2802379_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="67" mass="6809" checksum="C4B3C23A1AEDA809" modified="2017-05-10" version="1">MASDTTRRQFLIAASAAATFTVVAGCSEPEEDPPGYQFEDVPEDGDTAMAASPEQESGGADPAAEGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ27</accession>
    <name>A0A1U7EZ27_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">PrpD family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_4820A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PrpD family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324113.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ27"/>
    <dbReference type="STRING" id="348780.NP_4820A"/>
    <dbReference type="EnsemblBacteria" id="CAI50501">
        <property type="protein sequence ID" value="CAI50501"/>
        <property type="gene ID" value="NP_4820A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702187"/>
    <dbReference type="KEGG" id="nph:NP_4820A"/>
    <dbReference type="eggNOG" id="arCOG04285">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026574_3_0_2"/>
    <dbReference type="OrthoDB" id="43639at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4100.10">
        <property type="entry name" value="2-methylcitrate dehydratase PrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036148">
        <property type="entry name" value="MmgE/PrpD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042183">
        <property type="entry name" value="MmgE/PrpD_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005656">
        <property type="entry name" value="MmgE_PrpD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045337">
        <property type="entry name" value="MmgE_PrpD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045336">
        <property type="entry name" value="MmgE_PrpD_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16943:SF8">
        <property type="entry name" value="2-METHYLCITRATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16943">
        <property type="entry name" value="2-METHYLCITRATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19305">
        <property type="entry name" value="MmgE_PrpD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03972">
        <property type="entry name" value="MmgE_PrpD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103378">
        <property type="entry name" value="2-methylcitrate dehydratase PrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MmgE/PrpD N-terminal" evidence="2">
        <location>
            <begin position="6"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="MmgE/PrpD C-terminal" evidence="3">
        <location>
            <begin position="255"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="414" mass="42313" checksum="077DE1E2D88D2170" modified="2017-05-10" version="1">MTSTAEVAAFAADVSYDGLPETTREAAKRRVLDAVGVALGSRDDERTAPVRRAVPRVTTDGCRLWGTTDAATASDAANHNATATAVGPGGVYLSPTLSAAYGPLAAVLAVAESRGVTGEETLAALAATQEIHGELALNAPLDEFHPASHGAIAGAAGVGRVLGLDAERIERAVGLAAGQALLAVGDDEYAALAVGASAATAAEAGLLAEAGATSPDTLAGPGGWHDLVGPFDLDFDPGCERVRDAAVLPYDVHPYAQTAIAAAVDLAADATLDPADIDTATVETFADAVPVVAPEAIAAALVDRELYRQPTARADLGPVAEAVETAADDDLTDRAAHGELPARVTVKLRDGAVHEVEHGRFDGHPAQPAPWGTVEEKFHALAGDRYDADRRADIVDTVRSLEAESATELALLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ33</accession>
    <name>A0A1U7EZ33_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Sensory rhodopsin II transducer</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">htrII</name>
        <name evidence="9" type="synonym">htr2</name>
        <name evidence="9" type="ordered locus">NP_4832A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ33"/>
    <dbReference type="STRING" id="348780.NP_4832A"/>
    <dbReference type="EnsemblBacteria" id="CAI50507">
        <property type="protein sequence ID" value="CAI50507"/>
        <property type="gene ID" value="NP_4832A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_4832A"/>
    <dbReference type="eggNOG" id="arCOG02320">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_18_2"/>
    <dbReference type="OrthoDB" id="8523at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06225">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11386">
        <property type="entry name" value="MCP_signal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.470">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.950">
        <property type="entry name" value="Methyl-accepting chemotaxis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004090">
        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040913">
        <property type="entry name" value="Htr2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32089:SF112">
        <property type="entry name" value="LYSOZYME-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32089">
        <property type="entry name" value="METHYL-ACCEPTING CHEMOTAXIS PROTEIN MCPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17909">
        <property type="entry name" value="Htr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00260">
        <property type="entry name" value="CHEMTRNSDUCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158212">
        <property type="entry name" value="Htr2 transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58104">
        <property type="entry name" value="Methyl-accepting chemotaxis protein (MCP) signaling domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0807">Transducer</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="8">
        <location>
            <begin position="84"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="8">
        <location>
            <begin position="164"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="7">
        <location>
            <begin position="229"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="466"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="506"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="128"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="516"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI50507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="534" mass="56635" checksum="6BF906FD5A39FE5C" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ63</accession>
    <name>A0A1U7EZ63_NATPD</name>
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            <fullName evidence="1">Transcription factor S</fullName>
        </recommendedName>
        <alternativeName>
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        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">tfs2</name>
        <name evidence="15" type="synonym">rpoM2</name>
        <name evidence="15" type="ordered locus">NP_4938A</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
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        <lineage>
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            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
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                <person name="Oesterhelt D."/>
            </authorList>
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            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="16">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="8 12">Belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EnsemblBacteria" id="CAI50560">
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        <property type="gene ID" value="NP_4938A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703162"/>
    <dbReference type="KEGG" id="nph:NP_4938A"/>
    <dbReference type="eggNOG" id="arCOG00579">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093932_3_2_2"/>
    <dbReference type="OrthoDB" id="72957at2157"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Znf_TFIIS"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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        </location>
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        <ligand>
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        <ligand>
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        <location>
            <position position="68"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
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        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
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        <location>
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            <label>2</label>
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        </source>
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            <dbReference type="EMBL" id="CAI50560.1"/>
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    <sequence length="107" mass="11675" checksum="3D71BA4ACCD01883" modified="2017-05-10" version="1">MQFCDDCGSMMHAQGEAMVCSSCGATTEKDEAKAAEFVSTDAQTDDDVIESSPDADFEGKPTADDIICDECGHGEAWYTIKQTGAADEPPTRFFKCKECGHRWRGYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
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            </authorList>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="176"/>
            <end position="205"/>
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    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
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        <location>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI50564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="481" mass="53777" checksum="809984761BB2E569" modified="2017-05-10" version="1">MNSDDSSCDRDSCGCGSNDEPTDESFHPMGEEWEEEMREKLEETEYDADLGMEMAEDAMRLIQGELSEAEFHDRYNDAVVEEFDVDDRPTAEAYEEEMESDDGGLFQRLTGEDGTVGKLSDFDPDTDQTRRDTMKKMGAGAAALGFSGVASAASPTDDEYGDNPVSDTGDDDDVQMGMVIDLERCDGCLECVTGCIEENQTSEGANWMYVLTYEDEQTEQENFLVRPCQHCSNAPCEKVCPVRARHTRDKDGLVLTNYEVCIGCRYCQVACPYGVNYFQWGEPDVPYEDLPGSENDYDELKEMPYEERQEHLKETDDHLHDERGQWNDMRPAQGTMGKCTFCPSRQDGHQGEEKVGTVACMEACDTAGMSAIHFGDVNDPDSRPNQFLEQRAEEALENEDGETNNVDIDEDWGESNTPWGGKLSAFKLLDDLGTQPNVTYLGNEPGPEAEQVEGPVTYEQIGVQDDRKEHLDQLTFGTGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZC5</accession>
    <name>A0A1U7EZC5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5156A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324279.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZC5"/>
    <dbReference type="STRING" id="348780.NP_5156A"/>
    <dbReference type="EnsemblBacteria" id="CAI50669">
        <property type="protein sequence ID" value="CAI50669"/>
        <property type="gene ID" value="NP_5156A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702275"/>
    <dbReference type="KEGG" id="nph:NP_5156A"/>
    <dbReference type="eggNOG" id="arCOG04719">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_710987_0_0_2"/>
    <dbReference type="OrthoDB" id="204360at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055542">
        <property type="entry name" value="DUF7118"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23432">
        <property type="entry name" value="DUF7118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="128"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="382" mass="42372" checksum="E058913AA875F070" modified="2017-05-10" version="1">MTEPSTADGDAPAEALDDAATRLDQLQSELEADGLDRSTLETLADAYRSVETVLDRWEERATDWDDFEGYVKFRNDLAGTLDSIPDDIPDREAFVDADSHVKTGGVSKSLNESDFEAAREALAPVRARAEQLDELQAAESALRDARKAAKRRLETLEDRIAELERAIELGDADLDAPVEKLKDPMADYNEGIEAAFEAFLGDASAREVLSFIQTAAETPLVEYTSPPEALVSYLDTEPAGDHTLSELLEYAEYSPSKLAHYVDDPDRLKRRVATNRTYLEGLSAAPLQLDWPPAAAETLRFQVSERLPLAAKLADEPTVTALRTVSERTRRDDYEELRTAAAAREELTKTERQRLQSGAIEAELEAAREERTRLRSALGRTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZE5</accession>
    <name>A0A1U7EZE5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_5172A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324287.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZE5"/>
    <dbReference type="STRING" id="348780.NP_5172A"/>
    <dbReference type="EnsemblBacteria" id="CAI50677">
        <property type="protein sequence ID" value="CAI50677"/>
        <property type="gene ID" value="NP_5172A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702281"/>
    <dbReference type="KEGG" id="nph:NP_5172A"/>
    <dbReference type="eggNOG" id="arCOG04668">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1088211_0_0_2"/>
    <dbReference type="OrthoDB" id="303724at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054270">
        <property type="entry name" value="DUF7001"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22529">
        <property type="entry name" value="DUF7001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="250" mass="27895" checksum="22FA32A1C8F9ED17" modified="2017-05-10" version="1">MPDHVTLYRAPTTTADADEIAEWLDPRIDATVDVEPRLLGSLADDDLAEAFAASRVLSPYERETGNTMVGIVRYEERTLEAPERGGGVIYDGFDVQDALRRRLQSPMEELHIPLLDRRLGTWGTHDARWHKRVAVLGKPSLVSVPGLYEAPAKPEAYYKTKQSHAMMAGDAPPREVLENQVDGEFLVEDDPRTTEALKGYVLAAYHYLETGDAFCDVEGCRLYDAHRQPALLAAQLSEPAFCERHAARYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZM9</accession>
    <name>A0A1U7EZM9_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9 10">Cell division protein FtsZ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ftsZ2</name>
        <name evidence="8" type="synonym">ftsZ</name>
        <name evidence="14" type="ordered locus">NP_5324A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer. Polymerizes to form a dynamic ring structure in a strictly GTP-dependent manner. Interacts directly with several other division proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="8">Assembles at midcell at the inner surface of the cytoplasmic membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 10">Belongs to the FtsZ family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324362.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZM9"/>
    <dbReference type="STRING" id="348780.NP_5324A"/>
    <dbReference type="EnsemblBacteria" id="CAI50753">
        <property type="protein sequence ID" value="CAI50753"/>
        <property type="gene ID" value="NP_5324A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702728"/>
    <dbReference type="KEGG" id="nph:NP_5324A"/>
    <dbReference type="eggNOG" id="arCOG02201">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024865_0_1_2"/>
    <dbReference type="OrthoDB" id="371908at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051258">
        <property type="term" value="P:protein polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02201">
        <property type="entry name" value="FtsZ_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000008">
        <property type="entry name" value="Cell division protein FtsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000014">
        <property type="entry name" value="Cell division protein FtsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00909">
        <property type="entry name" value="FtsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000158">
        <property type="entry name" value="Cell_div_FtsZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020805">
        <property type="entry name" value="Cell_div_FtsZ_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045061">
        <property type="entry name" value="FtsZ/CetZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024757">
        <property type="entry name" value="FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00065">
        <property type="entry name" value="ftsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314:SF9">
        <property type="entry name" value="CELL DIVISION PROTEIN FTSZ 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314">
        <property type="entry name" value="CELL DIVISION PROTEIN FTSZ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12327">
        <property type="entry name" value="FtsZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00423">
        <property type="entry name" value="CELLDVISFTSZ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01134">
        <property type="entry name" value="FTSZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01135">
        <property type="entry name" value="FTSZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 8 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 8 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 10" id="KW-0717">Septation</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="12">
        <location>
            <begin position="34"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="13">
        <location>
            <begin position="226"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="341"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="385"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="42"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="129"/>
            <end position="131"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="NCBIfam" id="TIGR00065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAI50753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="401" mass="42247" checksum="7FE8A76A9E05523E" modified="2017-05-10" version="1">MQDIVNSALENAEAEQRDMDADLSGDGDEFGDPRIVIVGCGGAGNNTVNRLYNIGVDGADTIAINTDKQHLKMIEADTKILVGKSLTEGLGAGGEPDVGERATEMAQGTIKEVLGEADLVFVTAGMGGGTGTGAAPVVSKIAKEQGAIVVGMVSTPFNVERARTVKAEEGLEKLRNEADSIIVLDNNRLLDYVPNLPIGKAFSVMDQIIAETVKGISETITQPSLINLDYADMTSIMNQGGVAVMLVGETQDKNKTKEVVNDAMNHPLLDVDYRGASGGLVHITGGPDLTLKEAEGIAQNITERLEASANVIWGARIQDEYKGKVRVMAIMTGVQSAQILGPTTQKQADASRRAIEGVDDEEFSSTPPADHSADTGRDLGISETDGGRSEAEKNNGVDVVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILU8</accession>
    <name>Q3ILU8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3288A</name>
        <name evidence="3" type="ordered locus">NP_7012A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>PL23</name>
    </geneLocation>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
            <strain evidence="3">Gabara</strain>
            <plasmid evidence="3">PL23</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CR936259">
        <property type="protein sequence ID" value="CAI50922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILU8"/>
    <dbReference type="STRING" id="348780.NP_3288A"/>
    <dbReference type="EnsemblBacteria" id="CAI49735">
        <property type="protein sequence ID" value="CAI49735"/>
        <property type="gene ID" value="NP_3288A"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI50922">
        <property type="protein sequence ID" value="CAI50922"/>
        <property type="gene ID" value="NP_7012A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3288A"/>
    <dbReference type="KEGG" id="nph:NP_7012A"/>
    <dbReference type="HOGENOM" id="CLU_2217129_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="106" mass="11979" checksum="82EA6BDC09C54F4E" modified="2005-11-08" version="1">MAGDSCCIRTGDFYCNTAHSESMASNQCSKPVRTHRRQYTDGHEPLNTEERTVQQRNTALYANLTEYATMTLNLDKGQTVRVETYRGGIWIEPVDDTGTEVDIDVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILY3</accession>
    <name>Q3ILY3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Homolog to carboxyvinyl-carboxyphosphonate phosphorylmutase / homolog to isocitrate lyase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">prpB</name>
        <name evidence="1" type="ordered locus">NP_6212A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324484.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILY3"/>
    <dbReference type="EnsemblBacteria" id="CAI50886">
        <property type="protein sequence ID" value="CAI50886"/>
        <property type="gene ID" value="NP_6212A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694601"/>
    <dbReference type="KEGG" id="nph:NP_6212A"/>
    <dbReference type="HOGENOM" id="CLU_027389_3_2_2"/>
    <dbReference type="OrthoDB" id="9667at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016833">
        <property type="term" value="F:oxo-acid-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00377">
        <property type="entry name" value="ICL_PEPM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039556">
        <property type="entry name" value="ICL/PEPM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42905:SF5">
        <property type="entry name" value="CARBOXYVINYL-CARBOXYPHOSPHONATE PHOSPHORYLMUTASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42905">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13714">
        <property type="entry name" value="PEP_mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="313" mass="34026" checksum="EE2550EB65CB5599" modified="2005-11-08" version="1">MTEYGRQLRKQLDGDDQLVCPGVHDPLTAAVADTVGFDAIYMTGYGTSLSATGYPDAGFITMPEMISNAANIQERISVPLVADADNGYGNATNVVRTVREYIKAGVGAIHIEDQTFPKRCGHTEGRQVIPREEAVGKIQAAAEVRTERAEEFVLIARTDARGTGDGSLEEAIIRANAFLDAGADVAFVEGPTDESELQRIGAEVEGPLLYNFVGDLGTSPYVDLGTLEELGFEMVIFPIAATLATIASVHQNLQAFAEDPVDAMRQIDTQFNEQDIGSLHEFSGFPEVIEWEQRYLPDEDREKYEGSLGDSIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="124" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM34</accession>
    <name>Q3IM34_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphate transport system permease protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pstC2</name>
        <name evidence="9" type="synonym">abc15p2</name>
        <name evidence="9" type="ordered locus">NP_6102A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9 10">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Part of the binding-protein-dependent transport system for phosphate; probably responsible for the translocation of the substrate across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324433.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM34"/>
    <dbReference type="EnsemblBacteria" id="CAI50831">
        <property type="protein sequence ID" value="CAI50831"/>
        <property type="gene ID" value="NP_6102A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694671"/>
    <dbReference type="KEGG" id="nph:NP_6102A"/>
    <dbReference type="HOGENOM" id="CLU_033621_1_0_2"/>
    <dbReference type="OrthoDB" id="338493at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011864">
        <property type="entry name" value="Phosphate_PstC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02138">
        <property type="entry name" value="phosphate_pstC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42727:SF1">
        <property type="entry name" value="PHOSPHATE TRANSPORT SYSTEM PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42727">
        <property type="entry name" value="PHOSPHATE TRANSPORT SYSTEM PERMEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="299"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="102"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI50831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="332" mass="35642" checksum="529819BFAE854230" modified="2005-11-08" version="1">MATESQPDPGITKGSGTQADQIQNRRARRVLFGCATITVLTTLAIFFVLLDNATSYFWSSRLSHALLFGEPFQQEITFTEFFAGTRWAPEHATPAHGILPVVFGTLSITVGAAFVSIPIGTATALYVSEYAAPGVRSKLKPTLEVLAGIPTIVYGYFAIAFINPVVLGPIADALFGINIGRYSMLSGMLVVGIMTIPMVSSISEDAMQAVPDDLRNGAYALGATKYDVSVNVVVPAAISGIFASYILAVSRAVGETMAVTLAAGFNANITANPFDEIMTMTAYMVSMARGTSAVGTIEYQSLFAVGLLLFVMTLSMNLLNDYMKRRFQEEYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM52</accession>
    <name>Q3IM52_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UspA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_6060A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324415.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM52"/>
    <dbReference type="EnsemblBacteria" id="CAI50810">
        <property type="protein sequence ID" value="CAI50810"/>
        <property type="gene ID" value="NP_6060A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694696"/>
    <dbReference type="KEGG" id="nph:NP_6060A"/>
    <dbReference type="HOGENOM" id="CLU_049301_2_1_2"/>
    <dbReference type="OrthoDB" id="105697at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF6">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN UP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="5"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="157"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="307" mass="31481" checksum="978A49A957B6E27F" modified="2005-11-08" version="1">MILDTLLVPTDGSEPAVAAAQRAFELAVELDATVHILSVADSSIATGAGYSGDSTSIREQLRQTATDRATSLRDTASEYGLDATAVVREGIPAQEIVAYADDHAVDAITIGTSGRGGVTRAIIGSVADKVVRTSPVPVVTVTPAAARAETGVSGVDSMLVPTDGSDPATVAIEHALLVAAQLEATVHLLSVRNAGLEASLSAATADDAASHPQPREHIVEHLQTLAADAEANNLATTTAITDGSPAREIIAYADENDVDMIAMGTHGRGGFERAVVGSVTDAVIRESSVPVFSVRPDSVALEDVDET</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMK9</accession>
    <name>Q3IMK9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lipoate-protein ligase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">lpl1</name>
        <name evidence="2" type="ordered locus">NP_5116A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324259.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMK9"/>
    <dbReference type="STRING" id="348780.NP_5116A"/>
    <dbReference type="EnsemblBacteria" id="CAI50649">
        <property type="protein sequence ID" value="CAI50649"/>
        <property type="gene ID" value="NP_5116A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702890"/>
    <dbReference type="KEGG" id="nph:NP_5116A"/>
    <dbReference type="eggNOG" id="arCOG01939">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022986_5_0_2"/>
    <dbReference type="OrthoDB" id="43646at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16443">
        <property type="entry name" value="LplA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050664">
        <property type="entry name" value="Octanoyltrans_LipM/LipL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43679:SF2">
        <property type="entry name" value="OCTANOYL-[GCVH]:PROTEIN N-OCTANOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43679">
        <property type="entry name" value="OCTANOYLTRANSFERASE LIPM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21948">
        <property type="entry name" value="LplA-B_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="1">
        <location>
            <begin position="29"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="260" mass="28049" checksum="2CDC2F992CD929AA" modified="2005-11-08" version="1">MDWRLITEERLDGPTAMAYDEVAAETVAEGGPATVRLYRWTPSTVSLGYANDADIIDWEFCAANGIDVTRRATGGGAIYHDGVGEVAYSIIAPKADLPGDVTASYRQLLQPVLSAFEAAGAEVRFAPEPSEPLWQPMCYLRGLDPAHDLVGPDGRKVAGNAQHRTREAVVQHGSLTVAAKPQRHIGCFADPPVDPADFESTVTGIDVDRAALVEKLEASLAAWADADDGGWTSVERERAAELVDVKYDNDSWVRDRGDPT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-05-20" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMX6</accession>
    <name>RS4E_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein eS4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">30S ribosomal protein S4e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps4e</name>
        <name type="ordered locus">NP_4878A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324142.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMX6"/>
    <dbReference type="SMR" id="Q3IMX6"/>
    <dbReference type="STRING" id="348780.NP_4878A"/>
    <dbReference type="EnsemblBacteria" id="CAI50530">
        <property type="protein sequence ID" value="CAI50530"/>
        <property type="gene ID" value="NP_4878A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703183"/>
    <dbReference type="KEGG" id="nph:NP_4878A"/>
    <dbReference type="eggNOG" id="arCOG04093">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060400_0_0_2"/>
    <dbReference type="OrthoDB" id="372073at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06087">
        <property type="entry name" value="KOW_RPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00485">
        <property type="entry name" value="Ribosomal_eS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000876">
        <property type="entry name" value="Ribosomal_eS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013845">
        <property type="entry name" value="Ribosomal_eS4_central_region"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038237">
        <property type="entry name" value="Ribosomal_eS4_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041982">
        <property type="entry name" value="Ribosomal_eS4_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013843">
        <property type="entry name" value="Ribosomal_eS4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018199">
        <property type="entry name" value="Ribosomal_eS4_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003312">
        <property type="entry name" value="PRK04313.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11581">
        <property type="entry name" value="30S/40S RIBOSOMAL PROTEIN S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11581:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00900">
        <property type="entry name" value="Ribosomal_S4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08071">
        <property type="entry name" value="RS4NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002116">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00528">
        <property type="entry name" value="RIBOSOMAL_S4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000081345" description="Small ribosomal subunit protein eS4">
        <location>
            <begin position="1"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="S4 RNA-binding" evidence="1">
        <location>
            <begin position="37"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="237" mass="25775" checksum="87C702822CBA7187" modified="2005-11-08" version="1">MTNHQKRLAVPKSWPVERKEEAYTTKAAAGPHGEEGVPLLIVLRDVLGYVDSKKEARYALEQDSILINGTAVSDERRPVGMFDILAFDEREEYYRIFPDEGGRLSLTPIDEDAAGSKLGKIVGKTQVAGGDTQLSLHDGETLLVEDATAYDTNDSIVVSNDGEEIVAHFTYEEGALVTAVSGAHAGRIGTIDEIQVTPGSSANNVLIDAENDDERFETVEEYVVVIDENFTDGGDDE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMY3</accession>
    <name>RL22_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL22</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L22</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl22</name>
        <name type="ordered locus">NP_4864A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein binds specifically to 23S rRNA. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="1">The globular domain of the protein is located near the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that lines the wall of the exit tunnel in the center of the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL22 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324135.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMY3"/>
    <dbReference type="SMR" id="Q3IMY3"/>
    <dbReference type="STRING" id="348780.NP_4864A"/>
    <dbReference type="EnsemblBacteria" id="CAI50523">
        <property type="protein sequence ID" value="CAI50523"/>
        <property type="gene ID" value="NP_4864A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703132"/>
    <dbReference type="KEGG" id="nph:NP_4864A"/>
    <dbReference type="eggNOG" id="arCOG04098">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083987_0_2_2"/>
    <dbReference type="OrthoDB" id="314984at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00336">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.10">
        <property type="entry name" value="Ribosomal protein L22/L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01331_A">
        <property type="entry name" value="Ribosomal_uL22_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001063">
        <property type="entry name" value="Ribosomal_uL22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057265">
        <property type="entry name" value="Ribosomal_uL22_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005721">
        <property type="entry name" value="Ribosomal_uL22_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036394">
        <property type="entry name" value="Ribosomal_uL22_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003260">
        <property type="entry name" value="PRK04223.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01038">
        <property type="entry name" value="uL22_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593:SF10">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00237">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54843">
        <property type="entry name" value="Ribosomal protein L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000243251" description="Large ribosomal subunit protein uL22">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="154" mass="17076" checksum="6AEA7AF6BB0622BC" modified="2005-11-08" version="1">MGISYSVDVDPDETAKAMLRERHMSHKHSKEIAREIKGKTAGEAVDYLEAVIDGDRSVPFKSHNTGVGHRNDIEGWDAGRYPEKASEAFLDLLENGINNADHQGFEGEEMVIEHVAAHKVGESPGQKPRAFGRASPWNTPQVDVELVLRTEDDE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q3INQ4</accession>
    <name>IF2B_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Translation initiation factor 2 subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">aIF2-beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">eIF-2-beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">eif2b</name>
        <name type="ordered locus">NP_4314A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotrimer composed of an alpha, a beta and a gamma chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2-beta/eIF-5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323864.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3INQ4"/>
    <dbReference type="SMR" id="Q3INQ4"/>
    <dbReference type="STRING" id="348780.NP_4314A"/>
    <dbReference type="EnsemblBacteria" id="CAI50248">
        <property type="protein sequence ID" value="CAI50248"/>
        <property type="gene ID" value="NP_4314A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703266"/>
    <dbReference type="KEGG" id="nph:NP_4314A"/>
    <dbReference type="eggNOG" id="arCOG01640">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026663_3_1_2"/>
    <dbReference type="OrthoDB" id="38099at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00232">
        <property type="entry name" value="eIF_2_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045196">
        <property type="entry name" value="IF2/IF5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004458">
        <property type="entry name" value="TIF2_bsu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002735">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016189">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016190">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_Zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003067">
        <property type="entry name" value="PRK03988.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001:SF3">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01873">
        <property type="entry name" value="eIF-5_eIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00653">
        <property type="entry name" value="eIF2B_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100966">
        <property type="entry name" value="Translation initiation factor 2 beta, aIF2beta, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75689">
        <property type="entry name" value="Zinc-binding domain of translation initiation factor 2 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000021656" description="Translation initiation factor 2 subunit beta">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="134" mass="15052" checksum="9D8EA8688ED75610" modified="2005-11-08" version="1">MGYEEQLDRALEETPDIEGTAARFSVPDPDVRQEGNATVYENFQPTIDRLDREESHVMKFLQNELGTSANIDERGRLRLTGEFRQARVEEAINDYVEGYVICPECGLPDTRLEKENGAEVLRCEACGARSPAGN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IP66</accession>
    <name>PRIS_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA primase small subunit PriS</fullName>
            <ecNumber evidence="1">2.7.7.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">priS</name>
        <name type="synonym">priA</name>
        <name type="ordered locus">NP_3990A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalytic subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. The small subunit contains the primase catalytic core and has DNA synthesis activity on its own. Binding to the large subunit stabilizes and modulates the activity, increasing the rate of DNA synthesis while decreasing the length of the DNA fragments, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA repair.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of a small subunit (PriS) and a large subunit (PriL).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic-type primase small subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323702.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IP66"/>
    <dbReference type="SMR" id="Q3IP66"/>
    <dbReference type="STRING" id="348780.NP_3990A"/>
    <dbReference type="EnsemblBacteria" id="CAI50086">
        <property type="protein sequence ID" value="CAI50086"/>
        <property type="gene ID" value="NP_3990A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703050"/>
    <dbReference type="KEGG" id="nph:NP_3990A"/>
    <dbReference type="eggNOG" id="arCOG04110">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056123_1_0_2"/>
    <dbReference type="OrthoDB" id="31125at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04860">
        <property type="entry name" value="AE_Prim_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.920.10">
        <property type="entry name" value="DNA primase, PRIM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00700">
        <property type="entry name" value="DNA_primase_sml_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002755">
        <property type="entry name" value="DNA_primase_S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014052">
        <property type="entry name" value="DNA_primase_ssu_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023639">
        <property type="entry name" value="DNA_primase_ssu_PriS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001639">
        <property type="entry name" value="PRK00419.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10536">
        <property type="entry name" value="DNA PRIMASE SMALL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01896">
        <property type="entry name" value="DNA_primase_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56747">
        <property type="entry name" value="Prim-pol domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-0639">Primosome</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000225641" description="DNA primase small subunit PriS">
        <location>
            <begin position="1"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00700"/>
        </source>
    </evidence>
    <sequence length="393" mass="42795" checksum="B5522315577B9408" modified="2005-11-08" version="1">MNGRTREYLKGRFGDHYRRASLSPPPAANEREWGYITWGDGGTTMVRHHSYLDVTGGGDLGGFLAGERPRHVYFSAGRYDDPGASSMGQKGWRGSDLVFDLDADHLPGIDPSETSYAAMLEACKGALSRLLDLLVEDFGFEPLAVVFSGGRGYHVHVRRDDVLELDRTARREIVEYVLGEGLDFDDIVSTQAVAGSAGRSSPAQKRTLPDGGWAGRVRTRLTTLFDEVLTMEEAAALDRLREFDGIGEGKAKAALTAARNNREELTKGNVDVHPAVYSIARELFETVVEAESAPIDEPVTTDINRLIRLPGSLHGGTGLEVQHLDRDAIDAFDPLSDAVPETFVGNEIAIYTQEPTTVELRGESFRLSEGVSSVPEYVGVFAMARGYASKAGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-09-11" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IPF4</accession>
    <name>SRP19_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Signal recognition particle 19 kDa protein</fullName>
            <shortName evidence="1">SRP19</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">srp19</name>
        <name type="ordered locus">NP_3812A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds directly to 7S RNA and mediates binding of the 54 kDa subunit of the SRP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. Archaeal SRP consists of a 7S RNA molecule of 300 nucleotides and two protein subunits: SRP54 and SRP19.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SRP19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323614.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IPF4"/>
    <dbReference type="SMR" id="Q3IPF4"/>
    <dbReference type="STRING" id="348780.NP_3812A"/>
    <dbReference type="EnsemblBacteria" id="CAI49997">
        <property type="protein sequence ID" value="CAI49997"/>
        <property type="gene ID" value="NP_3812A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703216"/>
    <dbReference type="KEGG" id="nph:NP_3812A"/>
    <dbReference type="eggNOG" id="arCOG01217">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_169299_1_0_2"/>
    <dbReference type="OrthoDB" id="56356at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048500">
        <property type="term" value="C:signal recognition particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006617">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.30">
        <property type="entry name" value="Signal recognition particle, SRP19-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00305">
        <property type="entry name" value="SRP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002778">
        <property type="entry name" value="Signal_recog_particle_SRP19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053394">
        <property type="entry name" value="SRP19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036521">
        <property type="entry name" value="SRP19-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022938">
        <property type="entry name" value="SRP19_arc-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041311">
        <property type="entry name" value="Sig_rec_Srp19_Halo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17453">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 19 KD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17453:SF0">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 19 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01922">
        <property type="entry name" value="SRP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69695">
        <property type="entry name" value="SRP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0733">Signal recognition particle</keyword>
    <feature type="chain" id="PRO_0000300749" description="Signal recognition particle 19 kDa protein">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00305"/>
        </source>
    </evidence>
    <sequence length="92" mass="10200" checksum="512C5DC532EC70E0" modified="2005-11-08" version="1">MVENVIWPAYLDANCTRNEGRRVAEDLAVEEPTVDEIASAVQQVGYDAVIERSKTYPREYEPRGRVVVKGADDASKSDLLEAVAAYVTAIRE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-08-10" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IPX1</accession>
    <name>PSB_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Proteasome subunit beta</fullName>
            <ecNumber evidence="1">3.4.25.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">20S proteasome beta subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Proteasome core protein PsmB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">psmB</name>
        <name type="ordered locus">NP_3472A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped at one or both ends by the proteasome regulatory ATPase, PAN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase T1B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.25.1" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323447.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IPX1"/>
    <dbReference type="SMR" id="Q3IPX1"/>
    <dbReference type="STRING" id="348780.NP_3472A"/>
    <dbReference type="MEROPS" id="T01.002"/>
    <dbReference type="EnsemblBacteria" id="CAI49827">
        <property type="protein sequence ID" value="CAI49827"/>
        <property type="gene ID" value="NP_3472A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703062"/>
    <dbReference type="KEGG" id="nph:NP_3472A"/>
    <dbReference type="eggNOG" id="arCOG00970">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_7_2_2"/>
    <dbReference type="OrthoDB" id="6330at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02113_A">
        <property type="entry name" value="Proteasome_B_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019983">
        <property type="entry name" value="Pept_T1A_Psome_bsu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03634">
        <property type="entry name" value="arc_protsome_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF0">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE SUBUNIT HSLV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-0647">Proteasome</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0888">Threonine protease</keyword>
    <keyword id="KW-0865">Zymogen</keyword>
    <feature type="propeptide" id="PRO_0000397384" description="Removed in mature form; by autocatalysis" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000397385" description="Proteasome subunit beta">
        <location>
            <begin position="45"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="239" mass="25318" checksum="66FDAE4F09F47A16" modified="2005-11-08" version="1" precursor="true">MRQPDSSLPRTGQDHTLSPYEPELGEVPSNDLSMADLDNVNKTGTTTIGISTAEGVVIATDMRASLGGRFVSNKNVQKVEQIHPSAALTLVGSVGGAQSFIRSLRAEVDLYEARRGEDISINALATLAGNFARGGPFFAINPILGGVDDEGHHVYSIDPAGGVMKDDYTVTGSGLTVAYGTLEREYEDDMTNEEAKRVAASGIKAAVERDTGSGNGVFLATVTDEDVEIKGHKDFDEVL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-30" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IRF6</accession>
    <name>RNH2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease HII</fullName>
            <shortName evidence="1">RNase HII</shortName>
            <ecNumber evidence="1">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rnhB</name>
        <name type="ordered locus">NP_2392A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNase HII family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322913.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IRF6"/>
    <dbReference type="SMR" id="Q3IRF6"/>
    <dbReference type="STRING" id="348780.NP_2392A"/>
    <dbReference type="EnsemblBacteria" id="CAI49287">
        <property type="protein sequence ID" value="CAI49287"/>
        <property type="gene ID" value="NP_2392A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703111"/>
    <dbReference type="KEGG" id="nph:NP_2392A"/>
    <dbReference type="eggNOG" id="arCOG04121">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036532_0_4_2"/>
    <dbReference type="OrthoDB" id="33866at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032299">
        <property type="term" value="C:ribonuclease H2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07180">
        <property type="entry name" value="RNase_HII_archaea_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.460:FF:000001">
        <property type="entry name" value="Ribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.460">
        <property type="entry name" value="Ribonuclease hii. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00052_A">
        <property type="entry name" value="RNase_HII_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004649">
        <property type="entry name" value="RNase_H2_suA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001352">
        <property type="entry name" value="RNase_HII/HIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024567">
        <property type="entry name" value="RNase_HII/HIII_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020787">
        <property type="entry name" value="RNase_HII_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023160">
        <property type="entry name" value="RNase_HII_hlx-loop-hlx_cap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00729">
        <property type="entry name" value="ribonuclease HII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954:SF23">
        <property type="entry name" value="RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01351">
        <property type="entry name" value="RNase_HII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51975">
        <property type="entry name" value="RNASE_H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000236283" description="Ribonuclease HII">
        <location>
            <begin position="1"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="RNase H type-2" evidence="2">
        <location>
            <begin position="2"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01319"/>
        </source>
    </evidence>
    <sequence length="212" mass="22105" checksum="06BC771AD544CCB8" modified="2005-11-08" version="1">MTPLVGVDEAGKGPVVGSMFVAAVRAPTEALPDGIDDSKRLTDDRREALADRLRDADRIDVAVVECQPADIDDANMNDLTVAAHAAALDGVAASGETVLCDAGDVDAGRFADRVAAAATLDVSVAAEHGADGSDDLVGAASIIAKSARERHVEQLSEQYGAVGSGYPSDPTTKAFLREAFEQEGGFPPCVRTSWSTCERIRAEAEQTDLDGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IRF7</accession>
    <name>Q3IRF7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">tRNA pseudouridine synthase Pus10</fullName>
            <ecNumber evidence="5">5.4.99.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">tRNA pseudouridine 54/55 synthase</fullName>
            <shortName evidence="5">Psi54/55 synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 8" type="primary">pus10</name>
        <name evidence="8" type="ordered locus">NP_2390A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Responsible for synthesis of pseudouridine from uracil-54 and uracil-55 in the psi GC loop of transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>uridine(54) in tRNA = pseudouridine(54) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:57876"/>
            <dbReference type="Rhea" id="RHEA-COMP:10193"/>
            <dbReference type="Rhea" id="RHEA-COMP:14141"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>uridine(55) in tRNA = pseudouridine(55) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42532"/>
            <dbReference type="Rhea" id="RHEA-COMP:10101"/>
            <dbReference type="Rhea" id="RHEA-COMP:10102"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the pseudouridine synthase Pus10 family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.25" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322912.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IRF7"/>
    <dbReference type="STRING" id="348780.NP_2390A"/>
    <dbReference type="EnsemblBacteria" id="CAI49286">
        <property type="protein sequence ID" value="CAI49286"/>
        <property type="gene ID" value="NP_2390A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701433"/>
    <dbReference type="KEGG" id="nph:NP_2390A"/>
    <dbReference type="eggNOG" id="arCOG01015">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028780_2_0_2"/>
    <dbReference type="OrthoDB" id="10348at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160148">
        <property type="term" value="F:tRNA pseudouridine(55) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2510:FF:000001">
        <property type="entry name" value="tRNA pseudouridine synthase Pus10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01893">
        <property type="entry name" value="Pus10_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005912">
        <property type="entry name" value="Pus10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039894">
        <property type="entry name" value="Pus10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048741">
        <property type="entry name" value="Pus10-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055174">
        <property type="entry name" value="Pus10_THUMP_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01213">
        <property type="entry name" value="pseudo_Pus10arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21568">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE PUS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21568:SF0">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE PUS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21238">
        <property type="entry name" value="Pus10_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22023">
        <property type="entry name" value="Pus10_THUMP_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="THUMP" evidence="7">
        <location>
            <begin position="35"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="216"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="354"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="412" mass="45693" checksum="4E2166EB8C1C42C5" modified="2005-11-08" version="1">MTVLDAARDALDSGPVCNACLGRLFADRSFGLTNEARGRSLRTTIALEDDEPYEEPEADCWVCEGICDRFEEFADRVVDALGETELYTYQVGTRVPPLVAENDRLLRAEAGLAEDAGEPLKTELNREVGRRVGARLDADVDFERPDVQFLLDLDADTVEVQRNSLAIYGRYRKLERDIPQTEWDRYDESVAELISPPFLSAFRGTEAVFHGAGREDVDALMLGTGRPFVLEIKQPRRRQADLEALREEAHDHADGKVEIENLTAVTYEMVERVKEHDGDKTYRATVEFGAAVDESDFEAALAELDGTTIEQRTPHRVDHRRADLVRERTVLDIDGELEADHAATVEVHGEGGLYIKELMSGDEGRTEPSLAGLLGVGATVTALDVLAVEGVDEPFLTDDYRLERDGEPVSGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IU61</accession>
    <name>Q3IU61_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoesterase</fullName>
            <ecNumber evidence="1">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0462A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallophosphoesterase superfamily. YfcE family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321958.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IU61"/>
    <dbReference type="STRING" id="348780.NP_0462A"/>
    <dbReference type="EnsemblBacteria" id="CAI48322">
        <property type="protein sequence ID" value="CAI48322"/>
        <property type="gene ID" value="NP_0462A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700864"/>
    <dbReference type="KEGG" id="nph:NP_0462A"/>
    <dbReference type="eggNOG" id="arCOG01141">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063749_3_2_2"/>
    <dbReference type="OrthoDB" id="19174at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00841">
        <property type="entry name" value="MPP_YfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041802">
        <property type="entry name" value="MPP_YfcE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000979">
        <property type="entry name" value="Phosphodiesterase_MJ0936/Vps29"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00040">
        <property type="entry name" value="yfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11124">
        <property type="entry name" value="VACUOLAR SORTING PROTEIN VPS29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="2">
        <location>
            <begin position="2"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU362039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="172" mass="18902" checksum="943A3749575CDB27" modified="2005-11-08" version="1">MLAVLSDTHGRDSPRLSGRTADAVAEASRVVHAGDFMTEAVLDAFEERGPLAAVYGNNATAAVRERLPAERIVVWEGLRIAVVHGHEHTETALAMFGRQSSADLVVFGHSHEPTFEDGTVPMLNPGSHADPRWHRPAHAELEWDDDEQVARGQLVEPAGEVFERFTVEPRRP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-11-13" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IU70</accession>
    <name>ENDA_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">tRNA-splicing endonuclease</fullName>
            <ecNumber evidence="2">4.6.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">tRNA-intron endonuclease</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">endA</name>
        <name type="ordered locus">NP_0444A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.</text>
            <dbReference type="EC" id="4.6.1.16"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.16" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321949.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IU70"/>
    <dbReference type="SMR" id="Q3IU70"/>
    <dbReference type="STRING" id="348780.NP_0444A"/>
    <dbReference type="EnsemblBacteria" id="CAI48313">
        <property type="protein sequence ID" value="CAI48313"/>
        <property type="gene ID" value="NP_0444A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702672"/>
    <dbReference type="KEGG" id="nph:NP_0444A"/>
    <dbReference type="eggNOG" id="arCOG01701">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_791347_0_0_2"/>
    <dbReference type="OrthoDB" id="46045at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000213">
        <property type="term" value="F:tRNA-intron lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000379">
        <property type="term" value="P:tRNA-type intron splice site recognition and cleavage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22363">
        <property type="entry name" value="tRNA-intron_lyase_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.20">
        <property type="entry name" value="tRNA intron endonuclease, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01834">
        <property type="entry name" value="EndA_long"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036167">
        <property type="entry name" value="tRNA_intron_Endo_cat-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006677">
        <property type="entry name" value="tRNA_intron_Endonuc_cat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006678">
        <property type="entry name" value="tRNA_intron_Endonuc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036740">
        <property type="entry name" value="tRNA_intron_Endonuc_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006676">
        <property type="entry name" value="tRNA_splic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023516">
        <property type="entry name" value="tRNA_splic_arch_long"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00324">
        <property type="entry name" value="endA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006794">
        <property type="entry name" value="PRK09300.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13070:SF0">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13070">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN34-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01974">
        <property type="entry name" value="tRNA_int_endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02778">
        <property type="entry name" value="tRNA_int_endo_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53032">
        <property type="entry name" value="tRNA-intron endonuclease catalytic domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55267">
        <property type="entry name" value="tRNA-intron endonuclease N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000309820" description="tRNA-splicing endonuclease">
        <location>
            <begin position="1"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="271"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="282"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01834"/>
        </source>
    </evidence>
    <sequence length="336" mass="36543" checksum="F5BA9A0C581055A6" modified="2005-11-08" version="1">MEGHLSDGLVEVGPNGRERYYDSSGYGRPDGAGVRLAPVEAAYLLARGDLDAVDGMGLEAFLADTPDLVVPFLVYRDLRERGFYVSPALEGWVADHAGIDFVVHPRGDGPWDGTVAYRVRVVDERGSVRLDSLGDVVLAVVDEESELTYLETDRPAVDGTSGLEADLTADGVLLEDRVLCWDAPPALYEQGFYGQRMDREDGPLQLSLLEAAHLAATGVLSVAGGAAAVEARGRDAEGERFDRRLTVYRTLRERGVVPKTGFKFGADFRTYADVDSVSELGHSELLVRVLPADATLSPRDVALDVRLAHGVRKRMVFALVDDGDEIRWVSVSRLTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IUD6</accession>
    <name>Q3IUD6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NAD-dependent glucose-6-phosphate dehydrogenase</fullName>
            <shortName evidence="4">Glc6PDH</shortName>
            <ecNumber evidence="4">1.1.1.388</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">azf</name>
        <name evidence="6" type="ordered locus">NP_0308A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the NAD-dependent oxidation of glucose 6-phosphate to 6-phosphogluconolactone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>D-glucose 6-phosphate + NAD(+) = 6-phospho-D-glucono-1,5-lactone + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38215"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="1.1.1.388"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Carbohydrate degradation; pentose phosphate pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.388" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321883.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IUD6"/>
    <dbReference type="STRING" id="348780.NP_0308A"/>
    <dbReference type="EnsemblBacteria" id="CAI48245">
        <property type="protein sequence ID" value="CAI48245"/>
        <property type="gene ID" value="NP_0308A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700817"/>
    <dbReference type="KEGG" id="nph:NP_0308A"/>
    <dbReference type="eggNOG" id="arCOG04704">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079334_1_0_2"/>
    <dbReference type="OrthoDB" id="311692at2157"/>
    <dbReference type="UniPathway" id="UPA00115"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004345">
        <property type="term" value="F:glucose-6-phosphate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009051">
        <property type="term" value="P:pentose-phosphate shunt, oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02046">
        <property type="entry name" value="Glc6PDH_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032884">
        <property type="entry name" value="Glc6PDH_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041293">
        <property type="entry name" value="G6PDh_Azf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43103:SF5">
        <property type="entry name" value="4-EPIMERASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_7G00360)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43103">
        <property type="entry name" value="NUCLEOSIDE-DIPHOSPHATE-SUGAR EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="3 4" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="5">
        <location>
            <begin position="5"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI48245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="255" mass="28809" checksum="D6973148C47D92A3" modified="2005-11-08" version="1">MDETVLLTGAKGSVGTAIREGLADEYDWRYLFHNPPPFDPDHPYLVGDVSDEELVAEACEDVGAVVHLAGDPRRDAPWDSVLENNIHGTKVLYDAAIEAGVEKFVFASSNHAVGHFETDRKPELYRTDDDFRLDGTEFPRPGNHYGISKATGEVIGRYYHDEYGIDVCNIRIGNLTKGHPPKEYERGQAMWLSYRDCAHLHDRALQADYDFEIVYGISDNDRKYYSLERAKDVLGYEPKDNSADYHFDGTPKDDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IUL0</accession>
    <name>Q3IUL0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Type IV pilus biogenesis complex membrane subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">pilC2</name>
        <name evidence="8" type="ordered locus">NP_0158A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321809.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IUL0"/>
    <dbReference type="STRING" id="348780.NP_0158A"/>
    <dbReference type="EnsemblBacteria" id="CAI48170">
        <property type="protein sequence ID" value="CAI48170"/>
        <property type="gene ID" value="NP_0158A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700777"/>
    <dbReference type="KEGG" id="nph:NP_0158A"/>
    <dbReference type="eggNOG" id="arCOG01810">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_428035_0_0_2"/>
    <dbReference type="OrthoDB" id="252907at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056569">
        <property type="entry name" value="ArlJ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018076">
        <property type="entry name" value="T2SS_GspF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35402">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00482">
        <property type="entry name" value="T2SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="245"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="292"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="333"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="366"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="512"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="567"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="594"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="domain" description="Type II secretion system protein GspF" evidence="7">
        <location>
            <begin position="140"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI48170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="615" mass="65426" checksum="15959C0FBAF552E7" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETD8</accession>
    <name>A0A1U7ETD8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Malate dehydrogenase (Oxaloacetate-decarboxylating)</fullName>
            <ecNumber evidence="10">1.1.1.40</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">maeB1</name>
        <name evidence="10" type="synonym">mdh1:pta1</name>
        <name evidence="10" type="ordered locus">NP_0132A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the malic enzymes family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.40" evidence="10"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321796.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETD8"/>
    <dbReference type="STRING" id="348780.NP_0132A"/>
    <dbReference type="EnsemblBacteria" id="CAI48157">
        <property type="protein sequence ID" value="CAI48157"/>
        <property type="gene ID" value="NP_0132A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702904"/>
    <dbReference type="KEGG" id="nph:NP_0132A"/>
    <dbReference type="eggNOG" id="arCOG00853">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012366_0_0_2"/>
    <dbReference type="OrthoDB" id="45556at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004473">
        <property type="term" value="F:malate dehydrogenase (decarboxylating) (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05311">
        <property type="entry name" value="NAD_bind_2_malic_enz"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NADP-dependent malic enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000095">
        <property type="entry name" value="NADP-dependent malic enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10750">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10380">
        <property type="entry name" value="Malic enzyme, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051674">
        <property type="entry name" value="Malate_Decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012301">
        <property type="entry name" value="Malic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037062">
        <property type="entry name" value="Malic_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012302">
        <property type="entry name" value="Malic_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045213">
        <property type="entry name" value="Malic_NAD-bd_bact_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012188">
        <property type="entry name" value="ME_PTA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042113">
        <property type="entry name" value="P_AcTrfase_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042112">
        <property type="entry name" value="P_AcTrfase_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002505">
        <property type="entry name" value="PTA_PTB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43237">
        <property type="entry name" value="NADP-DEPENDENT MALIC ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43237:SF4">
        <property type="entry name" value="NADP-DEPENDENT MALIC ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00390">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03949">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01515">
        <property type="entry name" value="PTA_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036684">
        <property type="entry name" value="ME_PTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01274">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00919">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="6 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Malic enzyme N-terminal" evidence="9">
        <location>
            <begin position="16"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Malic enzyme NAD-binding" evidence="8">
        <location>
            <begin position="161"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI48157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="757" mass="81563" checksum="5D9D7878C4A365CB" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETE3</accession>
    <name>A0A1U7ETE3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HTH domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0134A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321797.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETE3"/>
    <dbReference type="STRING" id="348780.NP_0134A"/>
    <dbReference type="EnsemblBacteria" id="CAI48158">
        <property type="protein sequence ID" value="CAI48158"/>
        <property type="gene ID" value="NP_0134A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700765"/>
    <dbReference type="KEGG" id="nph:NP_0134A"/>
    <dbReference type="eggNOG" id="arCOG03067">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2091370_0_0_2"/>
    <dbReference type="OrthoDB" id="10985at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12840">
        <property type="entry name" value="HTH_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="116" mass="12195" checksum="C445192295D3FC52" modified="2017-05-10" version="1">MSTETGGTVAHETEPAKSGEAVVGDDDVGATLALLADEEARALFRHTGKPKTIPELTADCGVARSTAYRKVRKLTAAGLLEPASADDDPNTATAYQRTVDALEIIVGEAETIEVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETF2</accession>
    <name>A0A1U7ETF2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted glycoprotein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0154A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321807.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETF2"/>
    <dbReference type="STRING" id="348780.NP_0154A"/>
    <dbReference type="EnsemblBacteria" id="CAI48168">
        <property type="protein sequence ID" value="CAI48168"/>
        <property type="gene ID" value="NP_0154A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700775"/>
    <dbReference type="KEGG" id="nph:NP_0154A"/>
    <dbReference type="eggNOG" id="arCOG06296">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136582_0_0_2"/>
    <dbReference type="OrthoDB" id="306663at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055690">
        <property type="entry name" value="DUF7266"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23928">
        <property type="entry name" value="DUF7266"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="153" mass="15950" checksum="8F18FE0C796A92ED" modified="2017-05-10" version="1">MRDRCVPPADRRAMSPVVGKAMEATLVVLYLGIVTAALYGGAVPEYRAAAGTELAERAAADAATDIERSIPPDVAAAETRTVVELPPTIAGEAYRVEADGDRLAVDHPNPGIEASVPLSLPDRVVAVDGTWNSGDRTVAVVTTTEDGLEVQLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETI4</accession>
    <name>A0A1U7ETI4_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7124 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0228A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321844.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETI4"/>
    <dbReference type="STRING" id="348780.NP_0228A"/>
    <dbReference type="EnsemblBacteria" id="CAI48205">
        <property type="protein sequence ID" value="CAI48205"/>
        <property type="gene ID" value="NP_0228A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700794"/>
    <dbReference type="KEGG" id="nph:NP_0228A"/>
    <dbReference type="eggNOG" id="arCOG03063">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150429_0_0_2"/>
    <dbReference type="OrthoDB" id="221805at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055548">
        <property type="entry name" value="DUF7124"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23439">
        <property type="entry name" value="DUF7124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7124" evidence="2">
        <location>
            <begin position="8"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="134" mass="15055" checksum="A6E0A3D4D049A84B" modified="2017-05-10" version="1">MTDDAERITLAFELAALERLADPAGVIRDTMGWTSHLGIISDEPTHLVRKRARDYGFNPDFLPGPRSRHESLPKVKGQPEHDADRYILVAAHDDIEDAAERHGWEYRPVAEAAETAGWVLATETDDDEEHTGWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETK0</accession>
    <name>A0A1U7ETK0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">Nicotinamide-nucleotide adenylyltransferase</fullName>
            <ecNumber evidence="6 7">2.7.7.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">NAD(+) diphosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">NAD(+) pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">NMN adenylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">nadM2</name>
        <name evidence="9" type="ordered locus">NP_0310A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>beta-nicotinamide D-ribonucleotide + ATP + H(+) = diphosphate + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:21360"/>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="EC" id="2.7.7.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from nicotinamide D-ribonucleotide: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the archaeal NMN adenylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.1" evidence="6 7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321884.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETK0"/>
    <dbReference type="STRING" id="348780.NP_0310A"/>
    <dbReference type="EnsemblBacteria" id="CAI48246">
        <property type="protein sequence ID" value="CAI48246"/>
        <property type="gene ID" value="NP_0310A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702967"/>
    <dbReference type="KEGG" id="nph:NP_0310A"/>
    <dbReference type="eggNOG" id="arCOG00972">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108783_0_0_2"/>
    <dbReference type="OrthoDB" id="264480at2157"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00600"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000309">
        <property type="term" value="F:nicotinamide-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00243">
        <property type="entry name" value="NMN_adenylyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006418">
        <property type="entry name" value="NMN_Atrans_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01527">
        <property type="entry name" value="arch_NMN_Atrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002243">
        <property type="entry name" value="PRK01153.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21342:SF0">
        <property type="entry name" value="BIFUNCTIONAL NMN ADENYLYLTRANSFERASE_NUDIX HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21342">
        <property type="entry name" value="PHOSPHOPANTETHEINE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 6 9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="2 6" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="8">
        <location>
            <begin position="5"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="NCBIfam" id="TIGR01527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI48246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="168" mass="19054" checksum="095A7183F4C71154" modified="2017-05-10" version="1">MRGLLIGRFQPFHLGHRYLIEQIDEAVDEVVIGIGSAGQSHTRENPFTAGERVHLVQDALEELDAKTYLIPIADIERNAMWVTHIETLCPEFDVVYSNNPFVERLFEEAGYEVRGTPLHERDRYCGSEIRRRMVAGDRWRPLVPDPVAAAVDDIGGVARLQKITDDDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETK3</accession>
    <name>A0A1U7ETK3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">O-acetylhomoserine aminocarboxypropyltransferase (Methionine synthase)</fullName>
            <ecNumber evidence="7">2.5.1.49</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">metY2</name>
        <name evidence="7" type="ordered locus">NP_0280A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.49" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321870.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETK3"/>
    <dbReference type="STRING" id="348780.NP_0280A"/>
    <dbReference type="EnsemblBacteria" id="CAI48231">
        <property type="protein sequence ID" value="CAI48231"/>
        <property type="gene ID" value="NP_0280A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702921"/>
    <dbReference type="KEGG" id="nph:NP_0280A"/>
    <dbReference type="eggNOG" id="arCOG00061">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018986_4_0_2"/>
    <dbReference type="OrthoDB" id="43458at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004124">
        <property type="term" value="F:cysteine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003961">
        <property type="term" value="F:O-acetylhomoserine aminocarboxypropyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071269">
        <property type="term" value="P:L-homocysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000033">
        <property type="entry name" value="Cystathionine gamma-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000035">
        <property type="entry name" value="O-succinylhomoserine sulfhydrylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006235">
        <property type="entry name" value="OAc-hSer/O-AcSer_sulfhydrylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01326">
        <property type="entry name" value="OAH_OAS_sulfhy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43797">
        <property type="entry name" value="HOMOCYSTEINE/CYSTEINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43797:SF2">
        <property type="entry name" value="HOMOCYSTEINE_CYSTEINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="427" mass="46361" checksum="DCD87EAF42C7BCAF" modified="2017-05-10" version="1">MFDRDDLYTDSLHAGQEADPTTGARAPPIYQTTSYEFDDAEHAADLFGLREAGNIYSRIMNPTNAVLEERIASLEGGVAALATSSGMASFDLATFILASAGDNIVSSSSLYGGTYTYLTHTVERRGVETRFVDTLDYEAYEEAIDEDTAFVHCETIGNPALVTPDLERLADIAHDNGVPLFVDNTFATPALCRPLEHGADLVWNSTTKWIHGSGTTVGGVLVDGGSFPWDEHADRFPEIAKPNPAYHGINFSETFGDAAFAYAARTRGLRDLGNQQSPFDAWTTVQGLETLPMRMERHCENAQAVAEFLDDHPEVSWVTYPGLEDHETHEEASEYLDGYGGMITFGLEDGYEAARTTVESTELASLLANVGDAKTLIIHPASTTHQQLTDEEKQASGVTDDLVRLSVGLEDTDSIIADLEAAIDEAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETP3</accession>
    <name>A0A1U7ETP3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF373 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0380A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321919.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETP3"/>
    <dbReference type="STRING" id="348780.NP_0380A"/>
    <dbReference type="EnsemblBacteria" id="CAI48281">
        <property type="protein sequence ID" value="CAI48281"/>
        <property type="gene ID" value="NP_0380A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700836"/>
    <dbReference type="KEGG" id="nph:NP_0380A"/>
    <dbReference type="eggNOG" id="arCOG04151">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048986_1_0_2"/>
    <dbReference type="OrthoDB" id="31282at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007254">
        <property type="entry name" value="DUF373"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38815:SF1">
        <property type="entry name" value="DUF373 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38815">
        <property type="entry name" value="HYPOTHETICAL MEMBRANE PROTEIN, CONSERVED, DUF373 FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04123">
        <property type="entry name" value="DUF373"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="211"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="287"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="338"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="370" mass="38752" checksum="E91E278B779A433F" modified="2017-05-10" version="1">MSTLVVCVDRDGGIDHETPVVGWETIRGLVTEIGIDDPEDSRVNCLLEALRVGRDLRDSGETAIVAVVSGGGEGVNSDRAVAEQVDELVDAHDPSSAIVVTDSANDERLVPIIESRVQVDAVDRVIVRQSHDIQSTYYLLKQFLGDEELRGTVLVPIGAGMLAFPLLLLLADSVAVAMASIAAVIGLFLLYKGLGIDDLVSTLPSRARTAFYSGQVSLVTYVVGAGLALIGVFAGGINAAGMSAESELLVAVRFLFDSVPWLAAAALAAATGRLIDELLAEQPVSTALMNLPFGVVAIGLVVRGFSGYLLERAGVFEPFVVPSFEAGPVAVEGFAVSIWGRLAIYIVAGVVVSLLGVVFTSQMNDAGERG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETQ2</accession>
    <name>A0A1U7ETQ2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Aconitate hydratase</fullName>
            <ecNumber evidence="7">4.2.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">citB1</name>
        <name evidence="7" type="ordered locus">NP_0404A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.2.1.3" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321931.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETQ2"/>
    <dbReference type="STRING" id="348780.NP_0404A"/>
    <dbReference type="EnsemblBacteria" id="CAI48293">
        <property type="protein sequence ID" value="CAI48293"/>
        <property type="gene ID" value="NP_0404A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702609"/>
    <dbReference type="KEGG" id="nph:NP_0404A"/>
    <dbReference type="eggNOG" id="arCOG01697">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006714_2_3_2"/>
    <dbReference type="OrthoDB" id="255at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003994">
        <property type="term" value="F:aconitate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000019">
        <property type="entry name" value="Aconitate hydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050926">
        <property type="entry name" value="Aconitase/IPM_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006250">
        <property type="entry name" value="Aconitase_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01342">
        <property type="entry name" value="acon_putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001614">
        <property type="entry name" value="PRK00402.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005558">
        <property type="entry name" value="PRK07229.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43160">
        <property type="entry name" value="ACONITATE HYDRATASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43160:SF4">
        <property type="entry name" value="ACONITATE HYDRATASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="5">
        <location>
            <begin position="7"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="6">
        <location>
            <begin position="528"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="633"/>
            <end position="657"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="657" mass="71023" checksum="9EFEC41AAB7DB979" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETR1</accession>
    <name>A0A1U7ETR1_NATPD</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0432A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321943.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETR1"/>
    <dbReference type="STRING" id="348780.NP_0432A"/>
    <dbReference type="EnsemblBacteria" id="CAI48307">
        <property type="protein sequence ID" value="CAI48307"/>
        <property type="gene ID" value="NP_0432A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700854"/>
    <dbReference type="KEGG" id="nph:NP_0432A"/>
    <dbReference type="eggNOG" id="arCOG04689">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057816_0_0_2"/>
    <dbReference type="OrthoDB" id="205650at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055519">
        <property type="entry name" value="DUF7093"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23373">
        <property type="entry name" value="DUF7093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="278" mass="28920" checksum="7E921E1058C263EB" modified="2017-05-10" version="1">MGLQCSILGHSFEPDGVERERETQGSEVVTTEREIERCTRCGKERVVSESTEVTAVVDAEEVDLDDDASVDAPGEESAEAATGDAEGQIGPQPAGEDANTGGTGGEGSASGGTSVDATTETDDIDVASDADEDLPDDGGAEIIDETVDPAEDDEETARDVVAEAPTDDGAPEPDPDGEDAVILSDDTDGEAADELERAPGQWPDDADESTDTADSEPIAAAPDEDTEPVDEQSLSGITVPDGRIVCPDCDFSVQAHSGYREGDPCPDCSAWLESERNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETT4</accession>
    <name>A0A1U7ETT4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0480A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETT4"/>
    <dbReference type="STRING" id="348780.NP_0480A"/>
    <dbReference type="EnsemblBacteria" id="CAI48331">
        <property type="protein sequence ID" value="CAI48331"/>
        <property type="gene ID" value="NP_0480A"/>
    </dbReference>
    <dbReference type="GeneID" id="43500084"/>
    <dbReference type="KEGG" id="nph:NP_0480A"/>
    <dbReference type="eggNOG" id="arCOG06331">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_194229_1_0_2"/>
    <dbReference type="OrthoDB" id="224830at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055978">
        <property type="entry name" value="DUF7556"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24433">
        <property type="entry name" value="DUF7556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="55" mass="5877" checksum="20E5C0AACC9E1CC0" modified="2017-05-10" version="1">MSQNPATPVTVSDCEVMASVDDGDDGEQLVIAELCRDGAYLTMPTSSTVAVDEWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETU1</accession>
    <name>A0A1U7ETU1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Probable oxidoreductase (Short-chain dehydrogenase family)</fullName>
            <ecNumber evidence="3">1.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0522A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321988.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETU1"/>
    <dbReference type="STRING" id="348780.NP_0522A"/>
    <dbReference type="EnsemblBacteria" id="CAI48352">
        <property type="protein sequence ID" value="CAI48352"/>
        <property type="gene ID" value="NP_0522A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700884"/>
    <dbReference type="KEGG" id="nph:NP_0522A"/>
    <dbReference type="eggNOG" id="arCOG01259">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_0_2"/>
    <dbReference type="OrthoDB" id="24596at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005559">
        <property type="entry name" value="PRK07231.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24321">
        <property type="entry name" value="DEHYDROGENASES, SHORT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24321:SF8">
        <property type="entry name" value="ESTRADIOL 17-BETA-DEHYDROGENASE 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="253" mass="26487" checksum="A90390D62793B374" modified="2017-05-10" version="1">MRFENDTVFITGAGSGIGRATALKVAREGAFVVATDINEDGGRETVDAVEDAGGDAAFHELDVTDADEFKAVVETAADEYGLDAMINNAGVGHPPAYTEDVTETTFEYVLDVNLRGVWNGCQAALPHLKSQGEGAIVNVGSLASFLGLPKQAVYSLTKGAVLNLTRAIASEAGRDGVRANAVCPGFIETPLGDQFFQSKRDPEKAKAETEKQYPLGRLGEPEEVADAIAFLASDEASFVSGHGLVVDGGFSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU40</accession>
    <name>A0A1U7EU40_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0284 protein NP_0736A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cobT</name>
        <name evidence="2" type="ordered locus">NP_0736A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0284 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322095.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU40"/>
    <dbReference type="STRING" id="348780.NP_0736A"/>
    <dbReference type="EnsemblBacteria" id="CAI48459">
        <property type="protein sequence ID" value="CAI48459"/>
        <property type="gene ID" value="NP_0736A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702622"/>
    <dbReference type="KEGG" id="nph:NP_0736A"/>
    <dbReference type="eggNOG" id="arCOG04272">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053134_0_0_2"/>
    <dbReference type="OrthoDB" id="9136at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008939">
        <property type="term" value="F:nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01086">
        <property type="entry name" value="UPF0284"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002805">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036087">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003371">
        <property type="entry name" value="PRK04447.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38811">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38811:SF1">
        <property type="entry name" value="UPF0284 PROTEIN SLL1500"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52733">
        <property type="entry name" value="Nicotinate mononucleotide:5,6-dimethylbenzimidazole phosphoribosyltransferase (CobT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="343" mass="34511" checksum="29605B6CEA5CAD1E" modified="2017-05-10" version="1">MTRLVLCAGTSRTAEIDGISAAGATTDLMVHTPSADAEILTYGRPVRAPVVPVSPSGCPTPAAVTRAVAAELGVETTVVDAGLAEPTGAPTVTVGARPGDDIREPDPVPSAPGAFAAARQFGRSLPDDEIFLAETVPGGTTTALGVLTALGEADVLGDRTVSSSLPENPLSLKREVVAAGLSASSLSPGAAADEPQVALRRMGDPALAVVAGLAAGALETDTAVTLAGGTQLVAAAACLRHDGYEGPLSLATTAFIDADPTVDLDRAAGDLDLDVTVTDPGFSDDHPGMAAYNAGEAKEGVGMGGALALADRAELPMETVRSRFRTVYDRLLDAAPEQAVPEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU71</accession>
    <name>A0A1U7EU71_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9 10">Cell division protein FtsZ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ftsZ1</name>
        <name evidence="8" type="synonym">ftsZ</name>
        <name evidence="14" type="ordered locus">NP_0810A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer. Polymerizes to form a dynamic ring structure in a strictly GTP-dependent manner. Interacts directly with several other division proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="8">Assembles at midcell at the inner surface of the cytoplasmic membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 10">Belongs to the FtsZ family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322132.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU71"/>
    <dbReference type="STRING" id="348780.NP_0810A"/>
    <dbReference type="EnsemblBacteria" id="CAI48496">
        <property type="protein sequence ID" value="CAI48496"/>
        <property type="gene ID" value="NP_0810A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702727"/>
    <dbReference type="KEGG" id="nph:NP_0810A"/>
    <dbReference type="eggNOG" id="arCOG02201">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024865_0_1_2"/>
    <dbReference type="OrthoDB" id="371908at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051258">
        <property type="term" value="P:protein polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02201">
        <property type="entry name" value="FtsZ_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000023">
        <property type="entry name" value="Cell division protein FtsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00909">
        <property type="entry name" value="FtsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000158">
        <property type="entry name" value="Cell_div_FtsZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020805">
        <property type="entry name" value="Cell_div_FtsZ_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045061">
        <property type="entry name" value="FtsZ/CetZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024757">
        <property type="entry name" value="FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00065">
        <property type="entry name" value="ftsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314">
        <property type="entry name" value="CELL DIVISION PROTEIN FTSZ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314:SF3">
        <property type="entry name" value="MITOCHONDRIAL DIVISION PROTEIN FSZA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12327">
        <property type="entry name" value="FtsZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00423">
        <property type="entry name" value="CELLDVISFTSZ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01134">
        <property type="entry name" value="FTSZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01135">
        <property type="entry name" value="FTSZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 8 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 8 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 10" id="KW-0717">Septation</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="12">
        <location>
            <begin position="59"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="13">
        <location>
            <begin position="253"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="369"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="18"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="370"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="67"/>
            <end position="71"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="155"/>
            <end position="157"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="NCBIfam" id="TIGR00065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAI48496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="388" mass="40517" checksum="D290979DB17FB074" modified="2017-05-10" version="1">MDSIIEDAIDEAEEDGEASSGDATPTDSADAGTTDADVKSSGTMTDEELASVVKDLQTKITVVGCGGAGGNTVTRMAEAGIHGAKLVAANTDAQHLATEVEADEKILIGRQRTGGRGAGSVPKIGEEAAQENLEDITNSIDGSDMVFITAGLGGGTGTGSAPVVAQAAQDQGALTIAIVTIPFTAEGERRRANADAGLERLRAVADTVIVIPNDRLLDYAPNMPLQDAFKICDRVLMRSVKGMTELITKPGLVNVDFADVKTIMENGGVAMIGLGESDSENKAQDSIRSALRSPLLDVEFDGAQSALVNVVGGPDMSIEEAEGVVEEIYDRIDPDARIIWGASVDPEFDGKMETMIVVTGVESPQIYGKSEVERERAAAGDDDIDYVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUA1</accession>
    <name>A0A1U7EUA1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Aminopeptidase (Homolog to leucyl aminopeptidase / aminopeptidase T)</fullName>
            <ecNumber evidence="10">3.4.11.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">pepB2</name>
        <name evidence="10" type="ordered locus">NP_0872A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase M29 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-" evidence="10"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322163.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUA1"/>
    <dbReference type="STRING" id="348780.NP_0872A"/>
    <dbReference type="EnsemblBacteria" id="CAI48527">
        <property type="protein sequence ID" value="CAI48527"/>
        <property type="gene ID" value="NP_0872A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703012"/>
    <dbReference type="KEGG" id="nph:NP_0872A"/>
    <dbReference type="eggNOG" id="arCOG01888">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057697_0_0_2"/>
    <dbReference type="OrthoDB" id="145069at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1830.10">
        <property type="entry name" value="Thermophilic metalloprotease (M29)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052170">
        <property type="entry name" value="M29_Exopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035097">
        <property type="entry name" value="M29_N-terminal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000787">
        <property type="entry name" value="Peptidase_M29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34448">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34448:SF1">
        <property type="entry name" value="BLL6088 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02073">
        <property type="entry name" value="Peptidase_M29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144052">
        <property type="entry name" value="Thermophilic metalloprotease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="8 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI48527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="362" mass="40107" checksum="9C5AD76BE3D81C06" modified="2017-05-10" version="1">MDARIREHATIIVDHSTEVQPGDNVVISAPSEAEDLAVALHEVVGDRGATPVYLASDSRAQRAYLKATDDDFETPTHLKALYEETDVLIRVRGEANATEQSDVPPETQAARSKAYQPVQEVALSKRWCLTQYPTQGNAQLAGMSTEGYQNFVWDAVNKDWDAQRRHQEQMVEILDPASEVRIKSGDTTDVTMSLDGMKTLNDYAEKNLPGGEVFTAPVVDSVEGEVLFDMPLYHQGREITGAHLVFEDGEVVEHSADQNEGLLTEVLNTDPGASRLGELGIGMNRDIDEFTYNMLFDEKMGDTVHMAIGRAYDECVGEDREANESAVHLDMIVDMSDDSVIEVDGEVVQRDGTFRFEDDFEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUB8</accession>
    <name>A0A1U7EUB8_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">2-dehydropantoate 2-reductase</fullName>
            <ecNumber evidence="3 12">1.1.1.169</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 12">Ketopantoate reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">panE</name>
        <name evidence="15" type="synonym">apbA</name>
        <name evidence="15" type="ordered locus">NP_0942A</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the NAD(P)H-dependent reduction of ketopantoate into pantoic acid.</text>
    </comment>
    <comment type="function">
        <text evidence="12">Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(R)-pantoate + NAD(+) = 2-dehydropantoate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61292"/>
            <dbReference type="ChEBI" id="CHEBI:11561"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="10">
            <dbReference type="Rhea" id="RHEA:61294"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(R)-pantoate + NADP(+) = 2-dehydropantoate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16233"/>
            <dbReference type="ChEBI" id="CHEBI:11561"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.169"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="9">
            <dbReference type="Rhea" id="RHEA:16235"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the ketopantoate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.169" evidence="3 12"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322198.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUB8"/>
    <dbReference type="STRING" id="348780.NP_0942A"/>
    <dbReference type="EnsemblBacteria" id="CAI48562">
        <property type="protein sequence ID" value="CAI48562"/>
        <property type="gene ID" value="NP_0942A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702511"/>
    <dbReference type="KEGG" id="nph:NP_0942A"/>
    <dbReference type="eggNOG" id="arCOG04139">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031468_0_0_2"/>
    <dbReference type="OrthoDB" id="201845at2157"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008677">
        <property type="term" value="F:2-dehydropantoate 2-reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000017">
        <property type="entry name" value="2-dehydropantoate 2-reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003710">
        <property type="entry name" value="ApbA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050838">
        <property type="entry name" value="Ketopantoate_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013752">
        <property type="entry name" value="KPA_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013332">
        <property type="entry name" value="KPR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00745">
        <property type="entry name" value="apbA_panE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43765:SF2">
        <property type="entry name" value="2-DEHYDROPANTOATE 2-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43765">
        <property type="entry name" value="2-DEHYDROPANTOATE 2-REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02558">
        <property type="entry name" value="ApbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08546">
        <property type="entry name" value="ApbA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="5 12" id="KW-0521">NADP</keyword>
    <keyword evidence="7 12 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketopantoate reductase N-terminal" evidence="13">
        <location>
            <begin position="3"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Ketopantoate reductase C-terminal" evidence="14">
        <location>
            <begin position="172"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00056765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAI48562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="299" mass="30896" checksum="DFE37C374848B8B6" modified="2017-05-10" version="1">MRITIFGAGSLGSLVGGLLAREHDVVLVGRDPHVSRSRDSGLTIDGELATTVQLDARRDVPDRVGLAVICVKAFDTTTAAEALSGADIDACLSLQNGLGNEATLADQLAVPVLAGTCTYGALLESPGTVTCTGRGTVVLGARDGGSLPLADRVGDAFATAGLDVTVAEDMPRRLWEKLAVNAGINATTALARVDNGVLCSDDGNAVATAAARETAAVARATGIDLPTSTAVEATERVAATTAANVSSMRQDVRDGRRTEVDAINGYVVDKGNEFGVETPTNRTLCRLLRAWERGRGLRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUB9</accession>
    <name>A0A1U7EUB9_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Delta-aminolevulinic acid dehydratase</fullName>
            <ecNumber evidence="3 13">4.2.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">hemB</name>
        <name evidence="15" type="ordered locus">NP_0920A</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 13">
            <text>2 5-aminolevulinate = porphobilinogen + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="4.2.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Homooctamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the ALAD family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.24" evidence="3 13"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322187.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUB9"/>
    <dbReference type="STRING" id="348780.NP_0920A"/>
    <dbReference type="EnsemblBacteria" id="CAI48551">
        <property type="protein sequence ID" value="CAI48551"/>
        <property type="gene ID" value="NP_0920A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702806"/>
    <dbReference type="KEGG" id="nph:NP_0920A"/>
    <dbReference type="eggNOG" id="arCOG04300">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035731_0_0_2"/>
    <dbReference type="OrthoDB" id="8493at2157"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00318"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004655">
        <property type="term" value="F:porphobilinogen synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00384">
        <property type="entry name" value="ALAD_PBGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000019">
        <property type="entry name" value="Delta-aminolevulinic acid dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001731">
        <property type="entry name" value="ALAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030656">
        <property type="entry name" value="ALAD_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006762">
        <property type="entry name" value="PRK09283.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11458">
        <property type="entry name" value="DELTA-AMINOLEVULINIC ACID DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11458:SF0">
        <property type="entry name" value="DELTA-AMINOLEVULINIC ACID DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00490">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001415">
        <property type="entry name" value="Porphbilin_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00144">
        <property type="entry name" value="DALDHYDRTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01004">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00169">
        <property type="entry name" value="D_ALA_DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="6 13 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 13" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="9">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="9">
        <location>
            <position position="252"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAI48551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="326" mass="35787" checksum="8B2E21C21080768C" modified="2017-05-10" version="1">MDLTDRPRRLRADGVRDLVSETELHASDLIAPIFVDATTDERVEIESMPGHERVPVEEAVPRTEEVLSTGVESVIVFGVPESKDSEGSRAWADDGVVQRAVRAITDETDAYVITDVCFCEYTDHGHCGILESDAAEDPHLTMDNDATLELLRRTAVSHAEAGADMVAPSGMVDGMVGAIRNALDAAGYEQIPVMSYAAKYESAFYGPFRDAADGAPSFGDRRHYQMDPANAREARREVRLDVEQGADVLMVKPALPYLDIVSELRREFDRPVAAYNVSGEYAMLHAAADRGWLDRQSVAHESLLSIKRAGADLILTYFAESVAERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUE4</accession>
    <name>A0A1U7EUE4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Homolog to mandelate racemase / homolog to muconate lactonizing enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_0998A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322225.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUE4"/>
    <dbReference type="STRING" id="348780.NP_0998A"/>
    <dbReference type="EnsemblBacteria" id="CAI48590">
        <property type="protein sequence ID" value="CAI48590"/>
        <property type="gene ID" value="NP_0998A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701026"/>
    <dbReference type="KEGG" id="nph:NP_0998A"/>
    <dbReference type="eggNOG" id="arCOG01168">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030273_3_1_2"/>
    <dbReference type="OrthoDB" id="42605at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03316">
        <property type="entry name" value="MR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029065">
        <property type="entry name" value="Enolase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013342">
        <property type="entry name" value="Mandelate_racemase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013341">
        <property type="entry name" value="Mandelate_racemase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046945">
        <property type="entry name" value="RHMD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13794">
        <property type="entry name" value="ENOLASE SUPERFAMILY, MANDELATE RACEMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13794:SF58">
        <property type="entry name" value="MITOCHONDRIAL ENOLASE SUPERFAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13378">
        <property type="entry name" value="MR_MLE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02746">
        <property type="entry name" value="MR_MLE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00179">
        <property type="entry name" value="mandelate_racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00922">
        <property type="entry name" value="MR_MLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mandelate racemase/muconate lactonizing enzyme C-terminal" evidence="4">
        <location>
            <begin position="135"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI48590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="367" mass="40869" checksum="210A1B512B798B00" modified="2017-05-10" version="1">MQITGVNQYHLEHRLEEPFHPTWIPGYPQSTHELELFEIETDAGITGYGASPSFAGGLDYETPLELFLTGEDPHNVDGILGKLETVNLVGPRPWHIEMALWDIIGKDAGKPVCELFGAEQRAIPAYASSGEIQPADERIDYVEQRIDEGFDAVKLRVTETDHIETVRAVRESFPDLTLMVDANKGWAVRVMEEPTEWSFSDALEFARELDAVGGVEWLEEPLHRHDYDGYARLRDAVDVNIAGGEFNNGIHHFREFVDRGSLDILQPDAALATGIRRAVDVARMGEANGLQFVPHTWTNAVGFAANLHVMTAVGSPWCEYPMEPPWTPDVWSFMLEDGFEHDDGVVRAPSKPGLGIEIPDKVLEDAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUE6</accession>
    <name>A0A1U7EUE6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cysteine desulfurase</fullName>
            <ecNumber evidence="2">2.8.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">sufS1</name>
        <name evidence="7" type="ordered locus">NP_0936A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(sulfur carrier)-H + L-cysteine = (sulfur carrier)-SH + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:43892"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="EC" id="2.8.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="2.8.1.7" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322195.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUE6"/>
    <dbReference type="STRING" id="348780.NP_0936A"/>
    <dbReference type="EnsemblBacteria" id="CAI48559">
        <property type="protein sequence ID" value="CAI48559"/>
        <property type="gene ID" value="NP_0936A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702973"/>
    <dbReference type="KEGG" id="nph:NP_0936A"/>
    <dbReference type="eggNOG" id="arCOG00065">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003433_2_5_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031071">
        <property type="term" value="F:cysteine desulfurase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006534">
        <property type="term" value="P:cysteine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06453">
        <property type="entry name" value="SufS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010970">
        <property type="entry name" value="Cys_dSase_SufS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01979">
        <property type="entry name" value="sufS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43586">
        <property type="entry name" value="CYSTEINE DESULFURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43586:SF8">
        <property type="entry name" value="CYSTEINE DESULFURASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="6">
        <location>
            <begin position="30"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00050776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="413" mass="45755" checksum="4AFEA282A528B452" modified="2017-05-10" version="1">MSQQAEPLNVEAIREDFPILQREFDDTQLVYLDNAATTQTPEPVVETIADYYRRYNANVHRGIHQLSQEASIAYEEAHDRVAEFIGASGGREEVIFTKNTTESVNLVAYAWGLEELGPGDEIVLTEMEHHANLVTWQQIAKRTGAECKYIPITDEGRLDMDAARELITDETEMVAAVHVSNTLGTVNPVSELAAIAHDHDAYMFVDAAQSVPNRSVDVEAIDADFLAFSGHKMCGPTGIGVLYGKQHILESMEPYLYGGEMILKVTFEDSKWNELPWKYEAGTPVISQGIALAEACDYLDDIGMDRIERHEARLAEYAYDRLTEHDDVEVYGPPGDDRGGLVAFNVDSVHAHDLSSILNDSAVAIRAGDHCTQPLHDVLGATASARASFYIYNTKTEVDKLVEAVDDARQLFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUG3</accession>
    <name>A0A1U7EUG3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">DUF4341/DUF395 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">NP_0982A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322217.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUG3"/>
    <dbReference type="STRING" id="348780.NP_0982A"/>
    <dbReference type="EnsemblBacteria" id="CAI48582">
        <property type="protein sequence ID" value="CAI48582"/>
        <property type="gene ID" value="NP_0982A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701019"/>
    <dbReference type="KEGG" id="nph:NP_0982A"/>
    <dbReference type="eggNOG" id="arCOG04787">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122700_0_0_2"/>
    <dbReference type="OrthoDB" id="42259at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007272">
        <property type="entry name" value="Sulf_transp_TsuA/YedE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30574">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YEDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30574:SF1">
        <property type="entry name" value="SULPHUR TRANSPORT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04143">
        <property type="entry name" value="Sulf_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="22"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="161"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI48582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="185" mass="18774" checksum="AAC0BA4443CFA28E" modified="2017-05-10" version="1">MIAAFLGSGAVESLFPNGISRYAVGGVLVGLGTALIYAGTGIPAGASTFLESTLSYVSEQSRFQQYRPSRDWRVVFTGGIVAGAAIYALTFQSGFVTSSLYTPGTTGQLHDVGGFAAWTTDVSPWRLFVGGVLVGVGTRIGKGCTSGHGICGVGSVSRTSLIGVVTFLVVAILTANAVSALGVGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUH9</accession>
    <name>A0A1U7EUH9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1070A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322262.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUH9"/>
    <dbReference type="STRING" id="348780.NP_1070A"/>
    <dbReference type="EnsemblBacteria" id="CAI48626">
        <property type="protein sequence ID" value="CAI48626"/>
        <property type="gene ID" value="NP_1070A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701046"/>
    <dbReference type="KEGG" id="nph:NP_1070A"/>
    <dbReference type="HOGENOM" id="CLU_1891498_0_0_2"/>
    <dbReference type="OrthoDB" id="242857at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="134" mass="14653" checksum="6093DA8EF325D67C" modified="2017-05-10" version="1">MRSRRTVLGAAAVLASGFAGCFSTEAPDDQEPERTPDRNSEPVYVLRAAFAYQSDDLEPVLSTDDDAVADFETLYSLIIAVMDEHGTVAEEITAAEAERFKDLTEGLEYHGAGNPPGYYIDHEGTLISVSYDER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUI3</accession>
    <name>A0A1U7EUI3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CbtA family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_1098A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322276.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUI3"/>
    <dbReference type="STRING" id="348780.NP_1098A"/>
    <dbReference type="EnsemblBacteria" id="CAI48640">
        <property type="protein sequence ID" value="CAI48640"/>
        <property type="gene ID" value="NP_1098A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701051"/>
    <dbReference type="KEGG" id="nph:NP_1098A"/>
    <dbReference type="eggNOG" id="arCOG06353">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1136081_0_0_2"/>
    <dbReference type="OrthoDB" id="170869at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012666">
        <property type="entry name" value="CbtA_put"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09490">
        <property type="entry name" value="CbtA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="257" mass="26213" checksum="0F8780FB6BC762D0" modified="2017-05-10" version="1">MISDYLQRGVAAGLTAGLAYALYMVTVGNPLTDYIHDAGHSHDDGGHGHDDGGHGHEHAAEAHEHAGHAVSETTTAIVSAGSGVLWGIFLGGVFAVALYVFEPALPGVGDVKAYVLAGAGFFSASVMPWLVLPPAAPGAENIYGIETRLAIYLGLVVAGAVVSALAIAVYNRASRRHVGLGVLAGAVPVVAAVVAIPVLSPTVVSHPGLDAELVSTYQALAALSQAALWLLIAGVFCRLEGRFGGEPARQRDDAVAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUQ3</accession>
    <name>A0A1U7EUQ3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Isochorismatase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">entB1</name>
        <name evidence="4" type="ordered locus">NP_1274A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322363.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUQ3"/>
    <dbReference type="STRING" id="348780.NP_1274A"/>
    <dbReference type="EnsemblBacteria" id="CAI48728">
        <property type="protein sequence ID" value="CAI48728"/>
        <property type="gene ID" value="NP_1274A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702675"/>
    <dbReference type="KEGG" id="nph:NP_1274A"/>
    <dbReference type="eggNOG" id="arCOG01943">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068979_5_2_2"/>
    <dbReference type="OrthoDB" id="202119at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01014">
        <property type="entry name" value="nicotinamidase_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="entry name" value="Isochorismatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050272">
        <property type="entry name" value="Isochorismatase-like_hydrls"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43540:SF1">
        <property type="entry name" value="ISOCHORISMATASE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43540">
        <property type="entry name" value="PEROXYUREIDOACRYLATE/UREIDOACRYLATE AMIDOHYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="Isochorismatase-like hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase-like" evidence="3">
        <location>
            <begin position="14"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="58"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="196" mass="21684" checksum="A83AC756C7BB08F4" modified="2017-05-10" version="1">MPYRDYESLSLAESAIICVDVQQGFDRERWGERNNPDAERHIAELLAAWREAGHPVIHVRHDSTEPDSPLRPGRSGNEFKPAGEPEPGEPVVGKRVNSAFIGTELEALLRERGVRRPVFVGFTTDHCVSTSVRMAENLGFEPYVVSDATATFDRELGGTRYAAEQNHRLALAQLSDEFATVVETATLLAALAERKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUQ7</accession>
    <name>A0A1U7EUQ7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">2-oxoglutarate synthase subunit KorA</fullName>
            <ecNumber evidence="4">1.2.7.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">2-ketoglutarate oxidoreductase alpha chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">2-oxoglutarate-ferredoxin oxidoreductase subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">korA</name>
        <name evidence="10" type="ordered locus">NP_1232A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2 oxidized [2Fe-2S]-[ferredoxin] + 2-oxoglutarate + CoA = succinyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17297"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="EC" id="1.2.7.3"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterotetramer of the KorA, KorB, KorC and KorD subunits.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.3" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322343.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUQ7"/>
    <dbReference type="STRING" id="348780.NP_1232A"/>
    <dbReference type="EnsemblBacteria" id="CAI48707">
        <property type="protein sequence ID" value="CAI48707"/>
        <property type="gene ID" value="NP_1232A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702873"/>
    <dbReference type="KEGG" id="nph:NP_1232A"/>
    <dbReference type="eggNOG" id="arCOG01606">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017038_1_0_2"/>
    <dbReference type="OrthoDB" id="31112at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000022">
        <property type="entry name" value="2-oxoglutarate ferredoxin oxidoreductase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022367">
        <property type="entry name" value="2-oxoacid/accept_OxRdtase_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050722">
        <property type="entry name" value="Pyruvate:ferred/Flavod_OxRd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03710">
        <property type="entry name" value="OAFO_sf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32154:SF20">
        <property type="entry name" value="2-OXOGLUTARATE OXIDOREDUCTASE SUBUNIT KORA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32154">
        <property type="entry name" value="PYRUVATE-FLAVODOXIN OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate/ketoisovalerate oxidoreductase catalytic" evidence="8">
        <location>
            <begin position="13"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate flavodoxin/ferredoxin oxidoreductase pyrimidine binding" evidence="9">
        <location>
            <begin position="210"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00052359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00064882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00066947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00071398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00076968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00079587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI48707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="587" mass="64285" checksum="C1F3CE8CEBD4F9DF" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUQ9</accession>
    <name>A0A1U7EUQ9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Methylmalonyl-CoA mutase subunit A</fullName>
            <ecNumber evidence="3">5.4.99.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">mmcA2</name>
        <name evidence="3" type="ordered locus">NP_1226A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="5.4.99.2" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48704.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939798.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUQ9"/>
    <dbReference type="STRING" id="348780.NP_1226A"/>
    <dbReference type="EnsemblBacteria" id="CAI48704">
        <property type="protein sequence ID" value="CAI48704"/>
        <property type="gene ID" value="NP_1226A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702901"/>
    <dbReference type="KEGG" id="nph:NP_1226A"/>
    <dbReference type="eggNOG" id="arCOG04232">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009523_5_1_2"/>
    <dbReference type="OrthoDB" id="38408at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004494">
        <property type="term" value="F:methylmalonyl-CoA mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd03680">
        <property type="entry name" value="MM_CoA_mutase_ICM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.240">
        <property type="entry name" value="Methylmalonyl-CoA mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016176">
        <property type="entry name" value="Cbl-dep_enz_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006099">
        <property type="entry name" value="MeMalonylCoA_mutase_a/b_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006098">
        <property type="entry name" value="MMCoA_mutase_a_cat"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00641">
        <property type="entry name" value="acid_CoA_mut_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48101:SF1">
        <property type="entry name" value="METHYLMALONYL-COA MUTASE, LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48101">
        <property type="entry name" value="METHYLMALONYL-COA MUTASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01642">
        <property type="entry name" value="MM_CoA_mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51703">
        <property type="entry name" value="Cobalamin (vitamin B12)-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methylmalonyl-CoA mutase alpha/beta chain catalytic" evidence="2">
        <location>
            <begin position="41"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48704.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="556" mass="62042" checksum="5A87D4CF7B610A5D" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUR0</accession>
    <name>A0A1U7EUR0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Purine phosphoribosyltransferase (Adenine phosphoribosyltransferase, xanthine-guanine phosphoribosyltransferase)</fullName>
            <ecNumber evidence="2">2.4.2.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">apt2</name>
        <name evidence="2" type="ordered locus">NP_1254A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.4.2.-" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A1U7EUR0"/>
    <dbReference type="STRING" id="348780.NP_1254A"/>
    <dbReference type="EnsemblBacteria" id="CAI48718">
        <property type="protein sequence ID" value="CAI48718"/>
        <property type="gene ID" value="NP_1254A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702513"/>
    <dbReference type="KEGG" id="nph:NP_1254A"/>
    <dbReference type="eggNOG" id="arCOG00031">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086256_1_0_2"/>
    <dbReference type="OrthoDB" id="31493at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PRTase-like"/>
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    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050118">
        <property type="entry name" value="Pur/Pyrimidine_PRTase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006328">
        <property type="entry name" value="PRK08558.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43864:SF3">
        <property type="entry name" value="ADENINE PHOSPHORIBOSYLTRANSFERASE, FUSED TO N-TERMINAL DNA-BINDING DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43864">
        <property type="entry name" value="HYPOXANTHINE/GUANINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="1">
        <location>
            <begin position="111"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="238" mass="25568" checksum="CED774971D60E983" modified="2017-05-10" version="1">MNRSEKAALQLRAVDVLRTLKETRTYEELADETGLPAGDLNRYVNGHVLPSETRARDIVDSAGRELLATELNARIAVDEEGYVDNTRVVFDQSLLSLVPPVAAEGLGIDPPDTVLTAATDGITLAAAMARYFGARCAYAKQSRETAVEEFIEARQRLASGIEIDYYLPESAIDAGDSVLVVDDLIRSGETQELLLDIAGSADADVIGVFALIAVGDEGIQRARRHTDAPVGALVERNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUR1</accession>
    <name>A0A1U7EUR1_NATPD</name>
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        <submittedName>
            <fullName evidence="10">Prepilin/preflagellin peptidase</fullName>
            <ecNumber evidence="10">3.4.23.52</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">pibD</name>
        <name evidence="10" type="synonym">flaK</name>
        <name evidence="10" type="ordered locus">NP_1276A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.23.52" evidence="10"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48729.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_232503980.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUR1"/>
    <dbReference type="STRING" id="348780.NP_1276A"/>
    <dbReference type="EnsemblBacteria" id="CAI48729">
        <property type="protein sequence ID" value="CAI48729"/>
        <property type="gene ID" value="NP_1276A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702717"/>
    <dbReference type="KEGG" id="nph:NP_1276A"/>
    <dbReference type="eggNOG" id="arCOG02298">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065732_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052218">
        <property type="entry name" value="Preflagellin_Peptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009655">
        <property type="entry name" value="Preflagellin_peptidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000045">
        <property type="entry name" value="Prepilin_IV_endopep_pep"/>
    </dbReference>
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        <property type="entry name" value="PREFLAGELLIN PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36506:SF1">
        <property type="entry name" value="PREFLAGELLIN PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06847">
        <property type="entry name" value="Arc_PepC_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01478">
        <property type="entry name" value="Peptidase_A24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="40"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="107"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="136"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="305"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Prepilin type IV endopeptidase peptidase" evidence="8">
        <location>
            <begin position="21"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Preflagellin peptidase C-terminal" evidence="9">
        <location>
            <begin position="171"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="221"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="221"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF06847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI48729.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="333" mass="36047" checksum="3249BEDD0FB05254" modified="2017-05-10" version="1">MSSLLFGTATGPDLLRLAAVPAFAWAAYRDIETRRIPNRTWLPLLAVGIIALLWDTVAVTGFVTPTEVPTFERRRFVVQTLLSVGFVAPLGYIFWRIGGFGGADAKAIITLAVAFPVYPTYYVFQSAYPLYEAPLGVFSFTILSNAVVVGLAYPLVLAVRNLPRGEITPAMFIGRPVSVSAVTTEYGRLLETPDGFTRGGLDIDALRMYLQWRGSDLESLRAAPDDHRRTVPTDPNRPGDGAIDEWDTSPETGGSVAAADDPWGAEMFLDAHDAYGTTPEQLREGLAVLADAETEQVWISPGIPFIVPMFVGLVVAVTYGDVLFAVLDAFGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV14</accession>
    <name>A0A1U7EV14_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transducer protein Htr35</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">htr35</name>
        <name evidence="6" type="ordered locus">NP_1486A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322469.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV14"/>
    <dbReference type="STRING" id="348780.NP_1486A"/>
    <dbReference type="EnsemblBacteria" id="CAI48834">
        <property type="protein sequence ID" value="CAI48834"/>
        <property type="gene ID" value="NP_1486A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702849"/>
    <dbReference type="KEGG" id="nph:NP_1486A"/>
    <dbReference type="eggNOG" id="arCOG02318">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02347">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_654914_0_0_2"/>
    <dbReference type="OrthoDB" id="240684at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.950">
        <property type="entry name" value="Methyl-accepting chemotaxis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004090">
        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32089:SF112">
        <property type="entry name" value="LYSOZYME-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32089">
        <property type="entry name" value="METHYL-ACCEPTING CHEMOTAXIS PROTEIN MCPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00260">
        <property type="entry name" value="CHEMTRNSDUCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58104">
        <property type="entry name" value="Methyl-accepting chemotaxis protein (MCP) signaling domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="Methyl-accepting transducer" evidence="4">
        <location>
            <begin position="6"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="5">
        <location>
            <begin position="265"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI48834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="401" mass="42842" checksum="3A02A3B9ECBC9660" modified="2017-05-10" version="1">MDSQGEIDRLTEQAEAVQTHIEETVTASETQADAIAELSDDVSDLSATMEEIAASADQVAEAVADARELAESGVEDSRRAQSTAEETLAEADDLVEAIRSVSDRMDDIADIVDIIADIADQTNILALNANIEAARTDEGGEGFAVVADEVKSLADQTATNADEIQSMIDSLRSRTDDGLSAAEQTHDAVAETVDVVEEILSALEDIDDKIEAGADGTEEIAAANDSQAKTVDEIADRIDRIDERATEMQSQMSEAAALATDQREISGHMKGFINNLPGFAYRVENDEGWPMLFASDGIEELTGHDPAALLEGSISLGSDVIHPEDSDAVWQAVQESLDAGEPFDITYRVQTVEGDVRVVREQGRGVYGDDGDVVALEGYLSAVDAQTQRLFEDDAEGTQNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV30</accession>
    <name>A0A1U7EV30_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">HTH-10 family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_1564A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322507.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV30"/>
    <dbReference type="STRING" id="348780.NP_1564A"/>
    <dbReference type="EnsemblBacteria" id="CAI48873">
        <property type="protein sequence ID" value="CAI48873"/>
        <property type="gene ID" value="NP_1564A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701185"/>
    <dbReference type="KEGG" id="nph:NP_1564A"/>
    <dbReference type="eggNOG" id="arCOG02280">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090893_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056433">
        <property type="entry name" value="DmsR-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007050">
        <property type="entry name" value="HTH_bacterioopsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236:SF1">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24277">
        <property type="entry name" value="DmsR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04967">
        <property type="entry name" value="HTH_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="DmsR-like N-terminal" evidence="4">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="HTH bat-type" evidence="3">
        <location>
            <begin position="158"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI48873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="215" mass="23471" checksum="D851A3D7FCE50EA0" modified="2017-05-10" version="1">MSAGIRAEVRVDGGGSCPVAVASADAGTPTYSVARSVTGSDPDRVTEEFMLAAEGTPDAGTDLEAVFDYGSKTMYRFSRERGVGCPCEVIEVEDCPVVDIHTRDGNLHLVFHAADMETLQEVIFSLRERFPSVDVQRLLRDQHEAPEDNLILLDRSRLTDRQREVLETAHEMGYFEHPMGANAGEVADELDITTSTFSEHLAAAQSKLLDAILDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV39</accession>
    <name>A0A1U7EV39_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1560A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322505.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV39"/>
    <dbReference type="STRING" id="348780.NP_1560A"/>
    <dbReference type="EnsemblBacteria" id="CAI48871">
        <property type="protein sequence ID" value="CAI48871"/>
        <property type="gene ID" value="NP_1560A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701183"/>
    <dbReference type="KEGG" id="nph:NP_1560A"/>
    <dbReference type="HOGENOM" id="CLU_2893354_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="62" mass="6960" checksum="9CFA5795799C2955" modified="2017-05-10" version="1">MAERERDARSLFLSVTGETSVTERQQEDGGTGSRLVDDEPQTEFQAEDGLDDTYPDIDVEHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV70</accession>
    <name>A0A1U7EV70_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7350 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_1654A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322552.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV70"/>
    <dbReference type="STRING" id="348780.NP_1654A"/>
    <dbReference type="EnsemblBacteria" id="CAI48918">
        <property type="protein sequence ID" value="CAI48918"/>
        <property type="gene ID" value="NP_1654A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701213"/>
    <dbReference type="KEGG" id="nph:NP_1654A"/>
    <dbReference type="eggNOG" id="arCOG04511">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043006_0_0_2"/>
    <dbReference type="OrthoDB" id="156174at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2480">
        <property type="entry name" value="Periplasmic metal-binding protein Tp34-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055774">
        <property type="entry name" value="DUF7350"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038482">
        <property type="entry name" value="Tp34-type_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24041">
        <property type="entry name" value="DUF7350"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7350" evidence="2">
        <location>
            <begin position="317"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="437" mass="47516" checksum="B98015AE337DECFE" modified="2017-05-10" version="1">MNRRQFLAAGALVAAAGCLGPDDAEPPAAVDGQGASGRPQPDAKPDAVYMPTHREAMVHLPVAEAGPYRLSPMVAFAHEFWLVTGSETERVDASDPGLHVMVTPWDAETGQVLPVDTGGEMRIYRDENRIDQRAPWPMISQTMGFHFGDNIPIQEPGTYTVEVDVNPAGVRTTGDFDGRFMESATATFEFDYDDAVREELVEGTTFVDADRWGERGALEPMGEPDRSDHGEHDHGDGGDHNGDHSDHNGDHSDHNGDHSDHNGDHSDHNGDHSDHNGDHSDHNGDHSDHNGDHSDHNGDHGEHDHHEMPFSALPRAEEYPGLDLGVHESGDARFVVRYAENHRLADSGGGYLLVSPRTPYNRVPLADMALSVRGTVTTDLEQTLDSEVGLHYGASADLTAGDAFDLVVETPPQVARHAGYETAFLDMPSMPVEVPDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVF7</accession>
    <name>A0A1U7EVF7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1820A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322633.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVF7"/>
    <dbReference type="STRING" id="348780.NP_1820A"/>
    <dbReference type="EnsemblBacteria" id="CAI49001">
        <property type="protein sequence ID" value="CAI49001"/>
        <property type="gene ID" value="NP_1820A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701263"/>
    <dbReference type="KEGG" id="nph:NP_1820A"/>
    <dbReference type="eggNOG" id="arCOG03655">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_176775_0_0_2"/>
    <dbReference type="OrthoDB" id="189721at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058303">
        <property type="entry name" value="DUF7990"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25952">
        <property type="entry name" value="DUF7990"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="98" mass="11600" checksum="FD674093B6791015" modified="2017-05-10" version="1">MDLPARIDSLLAELKLWARGLYHGMIRHPAYENIEKAAEDLEDEFMLACFPDAFGIPSPVSYYTAELLPYLTDDFEAWQRRMWDRDSLIERKGQQYHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVH8</accession>
    <name>A0A1U7EVH8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Metallophosphoesterase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">NP_1868A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322657.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVH8"/>
    <dbReference type="STRING" id="348780.NP_1868A"/>
    <dbReference type="EnsemblBacteria" id="CAI49025">
        <property type="protein sequence ID" value="CAI49025"/>
        <property type="gene ID" value="NP_1868A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701283"/>
    <dbReference type="KEGG" id="nph:NP_1868A"/>
    <dbReference type="eggNOG" id="arCOG06201">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_604981_0_0_2"/>
    <dbReference type="OrthoDB" id="328870at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008758">
        <property type="term" value="F:UDP-2,3-diacylglucosamine hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009245">
        <property type="term" value="P:lipid A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043461">
        <property type="entry name" value="LpxH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34990:SF2">
        <property type="entry name" value="BLL8164 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34990">
        <property type="entry name" value="UDP-2,3-DIACYLGLUCOSAMINE HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="213"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="264"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="8">
        <location>
            <begin position="11"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="415"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI49025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="439" mass="49430" checksum="098DDC8AEDA24E2D" modified="2017-05-10" version="1">MEETADDRVYYVISDLHIGGDEQLEEVPFLDELLAFLERLRATDDDIELVINGDAFGLWEFTGLDGVEKFDHLVATYPKLFEQLRATGEQVQITLLPGNHDHELAAYDAYVERFAEYNVDLIQGQSIERRVGDHVVHFEHGHQRDANNHIEDWGNPYDKPLGYHYNTQVVSRAGHLSDRGRYNWLKDVQAVTPTERVPSWVFSKYFYREMNPLLRYALLPFLLIFNIAALVAIAAGMDLAGVISLPVEQTEAFLTELGPAGTTAWFLLVGNVVIAGLLVLVGIPLYLIRRDITKTVDRFGVFETDLTVDAETPYQEAAEEVFDANPDASVFCYGHTHRPMVQHVDGGALVNTGTWLKRLHRRDGVIGVLPPVFYPSYQLCAVRMEADGGDLVVDYEEIEKPLAASDELTFTERALTLGRKPEPELPDGVRIAGDKRRSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVI9</accession>
    <name>A0A1U7EVI9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Probable oxidoreductase (Short-chain dehydrogenase family)</fullName>
            <ecNumber evidence="3">1.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_1900A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322673.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVI9"/>
    <dbReference type="STRING" id="348780.NP_1900A"/>
    <dbReference type="EnsemblBacteria" id="CAI49041">
        <property type="protein sequence ID" value="CAI49041"/>
        <property type="gene ID" value="NP_1900A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701298"/>
    <dbReference type="KEGG" id="nph:NP_1900A"/>
    <dbReference type="eggNOG" id="arCOG01259">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_2_2"/>
    <dbReference type="OrthoDB" id="281764at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005559">
        <property type="entry name" value="PRK07231.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760:SF133">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="258" mass="27014" checksum="F442C1BD823E8784" modified="2017-05-10" version="1">MLEQFSVEGQTAVITGSSQGIGAVTAKRFADEGANVVVTSRTQEQIDEVADEINDSDRPGEAIAVECDVREREAVEALVEATVDEFGRIDSMINNAGASFMAGFDDISENGWKTIVDINLHGTFHCSQVAGQQMQSQDGGGTIVNFASVAGTMGSQYMSHYGAAKAAVVNLTTSLSAAYADENIRVNCIAPGLVGTPGVASQMGLDPSDVDREDVAKEMGLPEEIADIVQFLASDAASYIVGETITAQGVPPLEDSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVU0</accession>
    <name>A0A1U7EVU0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">3-isopropylmalate dehydrogenase</fullName>
            <ecNumber evidence="4">1.1.1.85</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">leuB</name>
        <name evidence="4" type="ordered locus">NP_2190A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.85" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322814.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVU0"/>
    <dbReference type="STRING" id="348780.NP_2190A"/>
    <dbReference type="EnsemblBacteria" id="CAI49186">
        <property type="protein sequence ID" value="CAI49186"/>
        <property type="gene ID" value="NP_2190A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702879"/>
    <dbReference type="KEGG" id="nph:NP_2190A"/>
    <dbReference type="eggNOG" id="arCOG01163">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031953_0_1_2"/>
    <dbReference type="OrthoDB" id="6813at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003862">
        <property type="term" value="F:3-isopropylmalate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004449">
        <property type="term" value="F:isocitrate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835">
        <property type="entry name" value="DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835:SF34">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="3">
        <location>
            <begin position="4"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="325" mass="33733" checksum="6A6D6AF8A6997032" modified="2017-05-10" version="1">MTETVLVVPGDGIGKEVVPAAVSVLDAVADFEFVEAEAGDHVKAETGEALPDETYERAAEADATLFGAAGETAADVILPLRQAVGSFANVRPARSYPGVEALRPETDLVFIRENTEGVYKGIESEIDDGVTTLTRVITDDASQKIAEYGFEYADENGYDDVTVAHKANVMRTTDGQFLDSVAAVAEAGGHDYEEALMDALAMHLLLRPEEYGVVICPNLAGDMLSDLAAGLVGGLGLLPSANVGEDNALFEPVHGSAPDIAGEGVANPAATILSGAMLLEHLGYGEEAATVRSAVESVLESGPRTPDLGGDASTEDVTAAIVDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVV8</accession>
    <name>A0A1U7EVV8_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Transcription initiation factor IIB</fullName>
            <shortName evidence="10">TFIIB</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">tfbA2</name>
        <name evidence="10" type="synonym">tfb</name>
        <name evidence="14" type="ordered locus">NP_2220A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre-initiation complex (DNA-TBP-TFIIB).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the TFIIB family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322828.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVV8"/>
    <dbReference type="STRING" id="348780.NP_2220A"/>
    <dbReference type="EnsemblBacteria" id="CAI49201">
        <property type="protein sequence ID" value="CAI49201"/>
        <property type="gene ID" value="NP_2220A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703234"/>
    <dbReference type="KEGG" id="nph:NP_2220A"/>
    <dbReference type="eggNOG" id="arCOG01981">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043736_0_0_2"/>
    <dbReference type="OrthoDB" id="7429at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20549">
        <property type="entry name" value="CYCLIN_TFIIB_archaea_like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20550">
        <property type="entry name" value="CYCLIN_TFIIB_archaea_like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023484">
        <property type="entry name" value="TFIIB_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023486">
        <property type="entry name" value="TFIIB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001658">
        <property type="entry name" value="PRK00423.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF13">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="Zn_Ribbon_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00782">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51134">
        <property type="entry name" value="ZF_TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIB-type" evidence="13">
        <location>
            <begin position="23"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="repeat" description="1" evidence="10">
        <location>
            <begin position="139"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="repeat" description="2" evidence="10">
        <location>
            <begin position="233"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00053882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAI49201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="322" mass="36009" checksum="1E9D838A65D300BD" modified="2017-05-10" version="1">MTDTTVRQYQRTDENESTSEDEDDLTCPECDGRLQADTEHGETVCADCGLVVEEDEIDRGPEWRAFDSAEKDEKSRVGAPTTKMMHDEGLSTNIGWQDKDAYGNSLSSRQRQKMQRLRTWNERFRTRDSKERNLKQALGEIERMASALGLPENVRETASVIYRRALDEDLLPGRSIEGVATASLYAAAKQARTPRSLDEVAKVSRVEKSEIARTYRYVVRELGLEIQPADPKSYVPRFASDIELSDEAERRARQLLDNAQEAGVVSGKSPVGLAAAAVYAASLLCNEKVTQKEVSEVADISEVTIRNRYHELLEAEQESGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVW7</accession>
    <name>A0A1U7EVW7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable rRNA methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2236A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVW7"/>
    <dbReference type="STRING" id="348780.NP_2236A"/>
    <dbReference type="EnsemblBacteria" id="CAI49209">
        <property type="protein sequence ID" value="CAI49209"/>
        <property type="gene ID" value="NP_2236A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701387"/>
    <dbReference type="KEGG" id="nph:NP_2236A"/>
    <dbReference type="eggNOG" id="arCOG00910">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074702_1_0_2"/>
    <dbReference type="OrthoDB" id="31271at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051720">
        <property type="entry name" value="rRNA_MeTrfase/Polyamine_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23290">
        <property type="entry name" value="RRNA N6-ADENOSINE-METHYLTRANSFERASE METTL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23290:SF0">
        <property type="entry name" value="RRNA N6-ADENOSINE-METHYLTRANSFERASE METTL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase small" evidence="1">
        <location>
            <begin position="50"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="212" mass="22550" checksum="A2419B5F88F07B00" modified="2017-05-10" version="1">MTDGNTGATRRRLAQELAVVAGFEDPTAPLEQYHTPPDLAAHIVHVADLQGDIVDRTVLDLGCGTGMLALGAALRGPERVVGVDIDADPLSTALENEQRVGAMTDVAWLRADATQLPVSPPDPTTVLMNPPFGAQSGNEGADRAFLETAADVAAVSYSVHNAGSESFVEAFADDNAGEVTHAFAAEFDIPRQFDHHTEQSSTIDTEVFRIEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVX0</accession>
    <name>A0A1U7EVX0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transducer protein Htr21</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">htr21</name>
        <name evidence="9" type="ordered locus">NP_2176A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVX0"/>
    <dbReference type="STRING" id="348780.NP_2176A"/>
    <dbReference type="EnsemblBacteria" id="CAI49179">
        <property type="protein sequence ID" value="CAI49179"/>
        <property type="gene ID" value="NP_2176A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_2176A"/>
    <dbReference type="eggNOG" id="arCOG02320">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_19_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Methyl-accepting chemotaxis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
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        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
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        <property type="entry name" value="LYSOZYME-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="METHYL-ACCEPTING CHEMOTAXIS PROTEIN MCPB"/>
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    </dbReference>
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        <property type="entry name" value="CHEMTRNSDUCR"/>
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    </dbReference>
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    <keyword evidence="1 3" id="KW-0807">Transducer</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="295"/>
            <end position="317"/>
        </location>
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        <location>
            <begin position="319"/>
            <end position="371"/>
        </location>
    </feature>
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        <location>
            <begin position="399"/>
            <end position="445"/>
        </location>
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        <location>
            <begin position="464"/>
            <end position="700"/>
        </location>
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        <location>
            <begin position="755"/>
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        </location>
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        <location>
            <begin position="366"/>
            <end position="404"/>
        </location>
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        <location>
            <begin position="440"/>
            <end position="471"/>
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        <location>
            <begin position="535"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="755"/>
            <end position="767"/>
        </location>
    </feature>
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            <begin position="768"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="785"/>
            <end position="795"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI49179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="815" mass="86936" checksum="D743D048BB59A3EA" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW19</accession>
    <name>A0A1U7EW19_NATPD</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2346A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="CAI49264">
        <property type="protein sequence ID" value="CAI49264"/>
        <property type="gene ID" value="NP_2346A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701416"/>
    <dbReference type="KEGG" id="nph:NP_2346A"/>
    <dbReference type="eggNOG" id="arCOG08168">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_564554_0_0_2"/>
    <dbReference type="OrthoDB" id="320276at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058376">
        <property type="entry name" value="DUF8063"/>
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    <dbReference type="Pfam" id="PF26259">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="207"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
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            <begin position="314"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="424"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="314"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="449"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI49264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="465" mass="50085" checksum="EE982D0591729098" modified="2017-05-10" version="1">MKRTLTVIFVFVAVVGCLTAPAAAQAETPTPTPDGDEVQTVEHQLGNSDVLEVDWTDRTAHITIEADSRARVSITDAGAIDNLGSGGSQRIGFETYTIPPGETREIQFTTASDPVITIQEGGEMVGASGDRQVLDIVTGAATVQLIQWSGFSGVAGTLLSVGLSVGLLKRRHQNSYRELTNDERHRIERDPVDGVLGRIKRFLSDHYVAIALAVAFGLYALLSAFGVVRGPVEFWNDLADAHRVIVAGTVATMLLAIAPAFALVSRVWNPDLKFVLDVDVDDMIQSARGVEPEDGGFAVYSGPPERIREMELRNADRDAGKSKSESPGGPAVVVRDFDPADNEADGTWPGLADDRELVSEQAKIRDNRSRLLDSAELGKNLLREISGISIASDIASTRAVDKAVRNALSTDSGAVDEILDEAAEGTRWEEYYTPGEVADDDRADHANESPDDQDDVTNDETDTNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW25</accession>
    <name>A0A1U7EW25_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">site-specific DNA-methyltransferase (cytosine-N(4)-specific)</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">NP_2358A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = an N(4)-methyl-2'-deoxycytidine in DNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16857"/>
            <dbReference type="Rhea" id="RHEA-COMP:11369"/>
            <dbReference type="Rhea" id="RHEA-COMP:13674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:85452"/>
            <dbReference type="ChEBI" id="CHEBI:137933"/>
            <dbReference type="EC" id="2.1.1.113"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.113" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322896.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW25"/>
    <dbReference type="STRING" id="348780.NP_2358A"/>
    <dbReference type="EnsemblBacteria" id="CAI49270">
        <property type="protein sequence ID" value="CAI49270"/>
        <property type="gene ID" value="NP_2358A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701420"/>
    <dbReference type="KEGG" id="nph:NP_2358A"/>
    <dbReference type="eggNOG" id="arCOG00115">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045086_0_0_2"/>
    <dbReference type="OrthoDB" id="241751at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015667">
        <property type="term" value="F:site-specific DNA-methyltransferase (cytosine-N4-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002941">
        <property type="entry name" value="DNA_methylase_N4/N6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017985">
        <property type="entry name" value="MeTrfase_CN4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001091">
        <property type="entry name" value="RM_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01555">
        <property type="entry name" value="N6_N4_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00508">
        <property type="entry name" value="S21N4MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00093">
        <property type="entry name" value="N4_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0680">Restriction system</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA methylase N-4/N-6" evidence="9">
        <location>
            <begin position="44"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI49270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="535" mass="61154" checksum="FE47F47598A75A9E" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW95</accession>
    <name>A0A1U7EW95_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Actin-like ATPase involved in cell morphogenesis</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2546A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322989.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW95"/>
    <dbReference type="STRING" id="348780.NP_2546A"/>
    <dbReference type="EnsemblBacteria" id="CAI49364">
        <property type="protein sequence ID" value="CAI49364"/>
        <property type="gene ID" value="NP_2546A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701480"/>
    <dbReference type="KEGG" id="nph:NP_2546A"/>
    <dbReference type="eggNOG" id="arCOG04656">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_757812_0_0_2"/>
    <dbReference type="OrthoDB" id="301490at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057331">
        <property type="entry name" value="Salactin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25229">
        <property type="entry name" value="Salactin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="360" mass="39130" checksum="0A82D1B37EC80B82" modified="2017-05-10" version="1">MSDDSTEEPDEDTTEEPDEDTTEATEGAAVGVKLGSTRTVLKTDDTVDKMLTCLATYEDALTGEERILYGEEAAREYPDTVRYMLRSGLPEDDENAEAAAKFFQAVVEANDVPEASGVVYAVPTMENEAGIRNLESVIESAAIGDVFTRAYPESLCGAVPVFGGVEALDEIFLSINLGSTNLEACAFRRGEALAPYATGGVTGSEVDRRIVSYIEEETQGRVNVDLTTAREYKEEHANFDAFEPFTDVIQQPGGGAHEFTIEDSVMDACDEYLDEVVDAVANEFLPELANDYMKVYQIALDETIVLTGGMACIPGLVDEFERRLSDELQRDIKADAADRPDLAAAEGAHEIASQLLKHQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWI4</accession>
    <name>A0A1U7EWI4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">UPF0098 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2694A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323063.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWI4"/>
    <dbReference type="STRING" id="348780.NP_2694A"/>
    <dbReference type="EnsemblBacteria" id="CAI49438">
        <property type="protein sequence ID" value="CAI49438"/>
        <property type="gene ID" value="NP_2694A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701527"/>
    <dbReference type="KEGG" id="nph:NP_2694A"/>
    <dbReference type="eggNOG" id="arCOG04702">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083918_3_0_2"/>
    <dbReference type="OrthoDB" id="28720at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00865">
        <property type="entry name" value="PEBP_bact_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008914">
        <property type="entry name" value="PEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005247">
        <property type="entry name" value="YbhB_YbcL/LppC-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01161">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="184" mass="19734" checksum="0765845641BB2F55" modified="2017-05-10" version="1">MRRRELIAAAPLVATAGCTIGGEPPDGSAFNIAIPDAEGVLPEQYTCDGEGVSPPLRITSVPSGTASLAVVGEWLRGYTPRTIWLLWGLPTTEPLEIPPGIGDEQTPDAFEEAVQGATEDGAIGYQPPCHESPDHDSYRFIAHALPEPLDLEPGAGRDDLDDHISETLTDVSSTSVQVRYERFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWK0</accession>
    <name>A0A1U7EWK0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HAD superfamily hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2774A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323103.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWK0"/>
    <dbReference type="STRING" id="348780.NP_2774A"/>
    <dbReference type="EnsemblBacteria" id="CAI49478">
        <property type="protein sequence ID" value="CAI49478"/>
        <property type="gene ID" value="NP_2774A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703029"/>
    <dbReference type="KEGG" id="nph:NP_2774A"/>
    <dbReference type="eggNOG" id="arCOG04221">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043473_1_2_2"/>
    <dbReference type="OrthoDB" id="25155at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006357">
        <property type="entry name" value="HAD-SF_hydro_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01460">
        <property type="entry name" value="HAD-SF-IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288">
        <property type="entry name" value="4-NITROPHENYLPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288:SF46">
        <property type="entry name" value="HALOACID DEHALOGENASE-LIKE HYDROLASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13344">
        <property type="entry name" value="Hydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13242">
        <property type="entry name" value="Hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000915">
        <property type="entry name" value="PGP-type_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="261" mass="27245" checksum="103A728EFDCCCB06" modified="2017-05-10" version="1">MEYAGAVLDLDGTIYRGSELLPGAAAAVRRLRELGIRPVFFSNNPTKSRSAYADRLSELGIDVGPESVLSAGTVTTRFLADEHGDGAIFLVGDDGLRRQFEAAGLHVVEEATAADVLVVSWTDKFDYGDLLAGYRALNSGATFYGTDPDRLIPAESGMAPGSGAIINAVGGVVGREPAKILGKPSPEARAAALSALDAPPERCLVIGDRLDTDIALGAAAGMTTVLVETGIDAEADIGESRRTPDYVIESLAELEQVLEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWP2</accession>
    <name>A0A1U7EWP2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DNA-directed RNA polymerase subunit K</fullName>
            <ecNumber evidence="3">2.7.7.6</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpoK</name>
        <name evidence="3" type="ordered locus">NP_2844A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.6" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323138.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWP2"/>
    <dbReference type="STRING" id="348780.NP_2844A"/>
    <dbReference type="EnsemblBacteria" id="CAI49513">
        <property type="protein sequence ID" value="CAI49513"/>
        <property type="gene ID" value="NP_2844A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703159"/>
    <dbReference type="KEGG" id="nph:NP_2844A"/>
    <dbReference type="eggNOG" id="arCOG01268">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112527_5_0_2"/>
    <dbReference type="OrthoDB" id="10567at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006360">
        <property type="term" value="P:transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006110">
        <property type="entry name" value="Pol_omega/Rpo6/RPB6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036161">
        <property type="entry name" value="RPB6/omega-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006111">
        <property type="entry name" value="Rpo6/Rpb6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002208">
        <property type="entry name" value="PRK01099.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47227">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE SUBUNIT K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47227:SF5">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01192">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000778">
        <property type="entry name" value="RpoK/RPB6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63562">
        <property type="entry name" value="RPB6/omega subunit-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="57" mass="6332" checksum="DDF6FE314F731649" modified="2017-05-10" version="1">MRGTNRYEKARILGARALQVSYGAPVLIETDQTEPILVAAEEYDADALPFTVHRGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWR2</accession>
    <name>A0A1U7EWR2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">CBS domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cbs3</name>
        <name evidence="5" type="ordered locus">NP_2894A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323163.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWR2"/>
    <dbReference type="STRING" id="348780.NP_2894A"/>
    <dbReference type="EnsemblBacteria" id="CAI49538">
        <property type="protein sequence ID" value="CAI49538"/>
        <property type="gene ID" value="NP_2894A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702581"/>
    <dbReference type="KEGG" id="nph:NP_2894A"/>
    <dbReference type="eggNOG" id="arCOG00601">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035203_0_0_2"/>
    <dbReference type="OrthoDB" id="9280at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd17780">
        <property type="entry name" value="CBS_pair_arch1_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04632">
        <property type="entry name" value="CBS_pair_arch1_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="7"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="63"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="128"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="230"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="183"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="195"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="406" mass="44033" checksum="150E490F024C3F2E" modified="2017-05-10" version="1">MDISAIVSTEFEELSPDTPVSKLTGVFDDPDVKGVVVSDDGDLVGVVTRRQLTTSHHSPEEKLASLVWHAPTVAPDENVREVARLMIDSGTQLLPVVEGDSMVGVVTADELLEAVQSFLEAATVGDAYSEELISIEPQTSFGKALHSFRDNRIAHLPVVDEGSAVGVVSLYDVTDIAVRATTQSQGGDAGGTDPFGGEISSSSGRARRGGFGAREGERDRLLDLPVRDVMVTPVRTVTPDETLEDAVAAMFDAGVSSLVVTRDGEPDGILTKTDILDALTWEAEGNRAVQLYGSDLLDDMNYDEVVSMIDAFDDMDGGTNVLDAKIHLHEHNETRRGTPLLLARIRLYTDGGLFMASGEGYGASHALGEAKDTLERRIRDNKTYAQTKKHPDEEFWDKRFGWWLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWS6</accession>
    <name>A0A1U7EWS6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Enoyl-CoA hydratase</fullName>
            <ecNumber evidence="2">4.2.1.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">fadA7</name>
        <name evidence="2" type="ordered locus">NP_2936A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.17" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323183.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWS6"/>
    <dbReference type="STRING" id="348780.NP_2936A"/>
    <dbReference type="EnsemblBacteria" id="CAI49559">
        <property type="protein sequence ID" value="CAI49559"/>
        <property type="gene ID" value="NP_2936A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702684"/>
    <dbReference type="KEGG" id="nph:NP_2936A"/>
    <dbReference type="eggNOG" id="arCOG00239">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_3_2"/>
    <dbReference type="OrthoDB" id="27846at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051683">
        <property type="entry name" value="Enoyl-CoA_Hydratase/Isomerase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42964">
        <property type="entry name" value="ENOYL-COA HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42964:SF1">
        <property type="entry name" value="POLYKETIDE BIOSYNTHESIS ENOYL-COA HYDRATASE PKSH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="272" mass="28531" checksum="8CCB9CAE5045DBDB" modified="2017-05-10" version="1">MERHDAASTEAVAVRVSEDDLVIRATIERPERQNALNDAVIEGLTAAFDLAAETPARVVVLRGAGGTFCAGGDIESMAAAIGSGSMAYREGFAGMRRLIEAAVDAPALTVAAVEGYCLAGGMGLAAACDVVIAADDTTFGLPEVDIGLFPAQALVPIMRTVTEKRAFKLLFTGEHIDAATAHDIGLTTAVVPADEFEAAIDTTVDDLTAASPAAVELGKEAFYNQREMGFREALDYMHEVVTLVAMSDDAESGINGYLTDSEPGWDRRTEGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWS8</accession>
    <name>A0A1U7EWS8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DUF849 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_2938A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323184.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWS8"/>
    <dbReference type="STRING" id="348780.NP_2938A"/>
    <dbReference type="EnsemblBacteria" id="CAI49560">
        <property type="protein sequence ID" value="CAI49560"/>
        <property type="gene ID" value="NP_2938A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701603"/>
    <dbReference type="KEGG" id="nph:NP_2938A"/>
    <dbReference type="eggNOG" id="arCOG06125">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065536_2_0_2"/>
    <dbReference type="OrthoDB" id="299212at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043720">
        <property type="term" value="F:3-keto-5-aminohexanoate cleavage activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008567">
        <property type="entry name" value="BKACE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37418:SF2">
        <property type="entry name" value="3-KETO-5-AMINOHEXANOATE CLEAVAGE ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37418">
        <property type="entry name" value="3-KETO-5-AMINOHEXANOATE CLEAVAGE ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05853">
        <property type="entry name" value="BKACE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="293" mass="31669" checksum="644F63035E77D1D3" modified="2017-05-10" version="1">MPLEQDSTKGNDPEKAVISAALTGALTTRDQCEHIPYTPAEIADDAAAAREAGAAVAHIHARTENGSPTFETEVYREIYEAVRDRTDILINFSTGALHEPVESRVDYIESVAPDIAALNMGSMNYAKYSESRGDFVFDMVFENSFGEIETMLAAMNEHGVTPELECFDTGHIGNVRPFLEDGTLDHPLHFSLIMGVLGGIPATVENLAHQVRQLPDDATWQVIGISEHQWQLVAAALSMGGNVRVGLEDNFYLPDGEMATNPELVAEAADLARTVGREPATPAEAADIMGVDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWT1</accession>
    <name>A0A1U7EWT1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 4 5">Thymidylate synthase</fullName>
            <shortName evidence="4">TS</shortName>
            <shortName evidence="4">TSase</shortName>
            <ecNumber evidence="1 4 5">2.1.1.45</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">hts</name>
        <name evidence="4 8" type="synonym">thyA</name>
        <name evidence="8" type="ordered locus">NP_2924A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>dUMP + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate = 7,8-dihydrofolate + dTMP</text>
            <dbReference type="Rhea" id="RHEA:12104"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57451"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="2.1.1.45"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Pyrimidine metabolism; dTTP biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.45" evidence="1 4 5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323177.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWT1"/>
    <dbReference type="STRING" id="348780.NP_2924A"/>
    <dbReference type="EnsemblBacteria" id="CAI49553">
        <property type="protein sequence ID" value="CAI49553"/>
        <property type="gene ID" value="NP_2924A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703260"/>
    <dbReference type="KEGG" id="nph:NP_2924A"/>
    <dbReference type="eggNOG" id="arCOG03214">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021669_0_0_2"/>
    <dbReference type="OrthoDB" id="50118at2157"/>
    <dbReference type="UniPathway" id="UPA00575"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004799">
        <property type="term" value="F:thymidylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006231">
        <property type="term" value="P:dTMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00351">
        <property type="entry name" value="TS_Pyrimidine_HMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.572.10">
        <property type="entry name" value="Thymidylate synthase/dCMP hydroxymethylase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00008">
        <property type="entry name" value="Thymidy_synth_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045097">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023451">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036926">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000398">
        <property type="entry name" value="Thymidylate_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03284">
        <property type="entry name" value="thym_sym"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11548">
        <property type="entry name" value="THYMIDYLATE SYNTHASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11548:SF1">
        <property type="entry name" value="THYMIDYLATE SYNTHASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00303">
        <property type="entry name" value="Thymidylat_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00108">
        <property type="entry name" value="THYMDSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55831">
        <property type="entry name" value="Thymidylate synthase/dCMP hydroxymethylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylate synthase/dCMP hydroxymethylase" evidence="7">
        <location>
            <begin position="2"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="280"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="303"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <feature type="binding site" description="in other chain" evidence="4">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="156"/>
            <end position="157"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="4">
        <location>
            <begin position="196"/>
            <end position="199"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="4">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="4">
        <location>
            <begin position="237"/>
            <end position="239"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="NCBIfam" id="TIGR03284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="342" mass="38665" checksum="B53F7E9F166E14C5" modified="2017-05-10" version="1">MQQYHDLVSDVLAGGTHKPNRTGVDTIASFSHHYEVDLGAGFPLLTTKRMDGYRWNSLIHELLWYLSGEEHIRTLREETKIWDAWADDEGHLDTAYGRFWRRFPVPDEPARLPGESWPDDSHRWTTTETTPDGTERQVFDQLAYVLDTLEENPHSRRLVVNAWHPANAAVSTLPPCHYTFVFNVQGDQLNVHLTQRSGDIALGVPFNLAAYSLLAHAVANRTAFEVGSFGHTIVDAHIYCGAGDRGAWYADKLPALQDRLNDIDDREEYVDVREWLESEAPAEADDEGGLDHVPGLLTQLSRSPRDRPEIEVADRPLDDLEYDDVQLHDYDPAPGISFSVAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWY0</accession>
    <name>A0A1U7EWY0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_3052A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323239.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWY0"/>
    <dbReference type="STRING" id="348780.NP_3052A"/>
    <dbReference type="EnsemblBacteria" id="CAI49617">
        <property type="protein sequence ID" value="CAI49617"/>
        <property type="gene ID" value="NP_3052A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701639"/>
    <dbReference type="KEGG" id="nph:NP_3052A"/>
    <dbReference type="eggNOG" id="arCOG07598">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143932_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045041">
        <property type="term" value="P:protein import into mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052604">
        <property type="entry name" value="Mito_Tim_assembly_helper"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016694">
        <property type="entry name" value="UCP017292"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008913">
        <property type="entry name" value="Znf_CHY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037274">
        <property type="entry name" value="Znf_CHY_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28082:SF1">
        <property type="entry name" value="HELPER OF TIM PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28082">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05495">
        <property type="entry name" value="zf-CHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017292">
        <property type="entry name" value="UCP017292_Znf_CHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161219">
        <property type="entry name" value="CHY zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51266">
        <property type="entry name" value="ZF_CHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CHY-type" evidence="4">
        <location>
            <begin position="12"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="114" mass="12830" checksum="7734C72A70104053" modified="2017-05-10" version="1">MRVHGYEVRGVGLDGETRCAHYGTERDVVAIKFACCATYYACHRCHEECTDHERSRWPLGRRDEKAVLCGVCGTELAVQEYLGVDSCPDCGARFNPGCNNHTHLYFEERPTDGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWY8</accession>
    <name>A0A1U7EWY8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GNAT family acetyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3070A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323248.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWY8"/>
    <dbReference type="STRING" id="348780.NP_3070A"/>
    <dbReference type="EnsemblBacteria" id="CAI49626">
        <property type="protein sequence ID" value="CAI49626"/>
        <property type="gene ID" value="NP_3070A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701645"/>
    <dbReference type="KEGG" id="nph:NP_3070A"/>
    <dbReference type="eggNOG" id="arCOG00830">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_4_2_2"/>
    <dbReference type="OrthoDB" id="129730at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43072:SF8">
        <property type="entry name" value="ACYLTRANSFERASE FABY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43072">
        <property type="entry name" value="N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="18"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="206" mass="22935" checksum="2B9BBA0424B0EB80" modified="2017-05-10" version="1">MDEIASPPVEEPTPPEDVSLRVATTRDAAAIADIYRPYIESTAATFREEPLEPSDVADEIDEPTYPWFVAERDGRVIGYAHAGELRKRAAYRWATELTVYLRESAHGAGVGSALYRLLLRTLARQGFVSAYGVVTLPNQSSVALHESLGFERNATFPAVGYKLGGWHDVAWYERQLRERPPEPSEPTPFEDCRSTPWLSALLGRDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX13</accession>
    <name>A0A1U7EX13_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3102A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323264.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX13"/>
    <dbReference type="STRING" id="348780.NP_3102A"/>
    <dbReference type="EnsemblBacteria" id="CAI49642">
        <property type="protein sequence ID" value="CAI49642"/>
        <property type="gene ID" value="NP_3102A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701652"/>
    <dbReference type="KEGG" id="nph:NP_3102A"/>
    <dbReference type="eggNOG" id="arCOG04642">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1507350_0_0_2"/>
    <dbReference type="OrthoDB" id="170224at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043827">
        <property type="entry name" value="DUF5804"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19120">
        <property type="entry name" value="DUF5804"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="154" mass="16801" checksum="37A56983BDC53B23" modified="2017-05-10" version="1">MARVCLVGADDVELRYELLSRETAREALATYNLTEPYENTVAAETVSLGSAVALLNDLNWYLVRFAESALVLEPSVSDTEWLSRTVAAGIRDDDISPAETGQLLKLYAVVDGTLYDPIYVTRDAMDGDPPTHEREAEDAGGTVVVRVAESEFEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXA8</accession>
    <name>A0A1U7EXA8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UPF0229 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3448A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323435.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXA8"/>
    <dbReference type="STRING" id="348780.NP_3448A"/>
    <dbReference type="EnsemblBacteria" id="CAI49815">
        <property type="protein sequence ID" value="CAI49815"/>
        <property type="gene ID" value="NP_3448A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701775"/>
    <dbReference type="KEGG" id="nph:NP_3448A"/>
    <dbReference type="eggNOG" id="arCOG04631">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049702_1_0_2"/>
    <dbReference type="OrthoDB" id="340824at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006698">
        <property type="entry name" value="UPF0229"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003713">
        <property type="entry name" value="PRK05325.2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30510">
        <property type="entry name" value="UPF0229 PROTEIN YEAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30510:SF2">
        <property type="entry name" value="UPF0229 PROTEIN YEAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04285">
        <property type="entry name" value="DUF444"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="439" mass="49404" checksum="EA6E3B91BC859877" modified="2017-05-10" version="1">MGRRDDLDRFREVGEERRQDLSEFIQYGDLGASGPDSVNVPIKIVDLPEFAYDPRDMGGVGQGQGGTPDVGDPVGEPEPQPGDGDDDGEDGEPGEEGGDHEYYEMDPEEFAAELDEELGLDLDPKGKKVIEETEGDFTDITRSGPASTLDFERLFKQGLKRKLAMDFDEEYLKAALKVDGWGPATVFEWARDQNIPVSRAWIDDAYASMPADERSKWDSIEEMTDDVDRESTATRIRREGVDEVPFRREDERYRYPEIIEEREKNVVVVNIRDVSGSMRESKRELVERTFTPLDWYLTGKYDNAEFVYIAHDADAWEVDREEFFGIRSGGGTRISSAYELASEILEAEYPWSEWNRYVFAAGDSENSSNDTENNVIPLMESIPANLHAYVETQPSGNAINATHAQELESHFEDDNVAVAYIEGPGDVLDAIETVLSAEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXD2</accession>
    <name>A0A1U7EXD2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0212 protein NP_3468A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3468A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0212 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323445.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXD2"/>
    <dbReference type="STRING" id="348780.NP_3468A"/>
    <dbReference type="EnsemblBacteria" id="CAI49825">
        <property type="protein sequence ID" value="CAI49825"/>
        <property type="gene ID" value="NP_3468A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701781"/>
    <dbReference type="KEGG" id="nph:NP_3468A"/>
    <dbReference type="eggNOG" id="arCOG02119">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_138334_0_0_2"/>
    <dbReference type="OrthoDB" id="63517at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01223">
        <property type="entry name" value="UPF0212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007564">
        <property type="entry name" value="UPF0212"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003035">
        <property type="entry name" value="PRK03922.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42199">
        <property type="entry name" value="UPF0212 PROTEIN MJ0068"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42199:SF1">
        <property type="entry name" value="UPF0212 PROTEIN TK1194"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04475">
        <property type="entry name" value="DUF555"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016934">
        <property type="entry name" value="UCP016934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="119" mass="12712" checksum="41CAC910DC58D08B" modified="2017-05-10" version="1">MSNYLVAMEAAWLVRDVEEIDDAIGVAVSEAGKRLNEADMDYVEVEVGSTPCPACGEPFDSAFVAADTALVGLGLEMTVFNADSDQHAARIAKSEIGGALRDVPLKVIQTFETEDDESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXF5</accession>
    <name>A0A1U7EXF5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF293 domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3506A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323464.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXF5"/>
    <dbReference type="STRING" id="348780.NP_3506A"/>
    <dbReference type="EnsemblBacteria" id="CAI49844">
        <property type="protein sequence ID" value="CAI49844"/>
        <property type="gene ID" value="NP_3506A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701791"/>
    <dbReference type="KEGG" id="nph:NP_3506A"/>
    <dbReference type="eggNOG" id="arCOG00610">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1514568_0_0_2"/>
    <dbReference type="OrthoDB" id="64432at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000944">
        <property type="entry name" value="Tscrpt_reg_Rrf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005104">
        <property type="entry name" value="WHTH_HrcA_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33221:SF15">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR YWGB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33221">
        <property type="entry name" value="WINGED HELIX-TURN-HELIX TRANSCRIPTIONAL REGULATOR, RRF2 FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03444">
        <property type="entry name" value="WHD_HrcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Winged helix-turn-helix transcription repressor HrcA DNA-binding" evidence="1">
        <location>
            <begin position="5"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="179" mass="19491" checksum="F35AB7E86A17794D" modified="2017-05-10" version="1">MSSIELTPSQKTILQELINLYRESESAVKGEDIAEKVDRNPGTIRNQMQSLKALQLVEGVPGPKGGYKPTATAYDALKIQEMEQAADVPFNHEGDLVEEANVQEIDLTSVHHPELCRAEIKLQGSMADFEEGDKISVGPTPLSKLLIEGELEGKDDTNNSLILTIDNMAAPAGDEDPLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXG9</accession>
    <name>A0A1U7EXG9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Major facilitator superfamily transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tp13</name>
        <name evidence="7" type="ordered locus">NP_3560A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323491.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXG9"/>
    <dbReference type="STRING" id="348780.NP_3560A"/>
    <dbReference type="EnsemblBacteria" id="CAI49871">
        <property type="protein sequence ID" value="CAI49871"/>
        <property type="gene ID" value="NP_3560A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703289"/>
    <dbReference type="KEGG" id="nph:NP_3560A"/>
    <dbReference type="eggNOG" id="arCOG00130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_62_1_2"/>
    <dbReference type="OrthoDB" id="29061at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061513">
        <property type="term" value="F:glucose 6-phosphate:phosphate antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051337">
        <property type="entry name" value="OPA_Antiporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43826">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE EXCHANGER SLC37A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43826:SF3">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE EXCHANGER SLC37A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="143"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="175"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="233"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="298"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="324"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="359"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="402"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="19"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="441" mass="46107" checksum="377107E74D72DE52" modified="2017-05-10" version="1">MIADTVSVLERPTRYRWIAWGMLALAFLLASVHRLSTAVLSEALTAEFGLTAAQLGTLHASFFIGYALVQIPAGVLVDAVGPRHVGTAGAAAVSVGAIGFVFSSTYPTALLARAVIGVGGGVIFVSTLRFASNWFRVNEFASVTGLTAGMAGFGSILATTPLAVVIGLAGWRSTFLWFAVFGLLVAAAVYVLVRRSPAELGFDTIRSVPSQPSVTVSETGQHLRALARDPEQWLLSLCFFATMGSILTLLGLWGVPYLVTVYGLDVTTASYYTLLGAIGMLIGGPAVGRVSDRLGRRYLPMVVGFGLFVLVLVPIPLLGRPPKAVIAGSYFFTGFFIGAAVLTLSVIKEQYPAGASGVATAAVVTAGFLGGTVLPALLGVALDTYRTGAFVGGTAVYTEFGYRVAFALLSGAVAVAFLCSLWLFLRRGDEPMHSARAADSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXJ9</accession>
    <name>A0A1U7EXJ9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">IS1341-type transposase ISNph10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">NP_3590A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323506.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXJ9"/>
    <dbReference type="STRING" id="348780.NP_3590A"/>
    <dbReference type="EnsemblBacteria" id="CAI49886">
        <property type="protein sequence ID" value="CAI49886"/>
        <property type="gene ID" value="NP_3590A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701815"/>
    <dbReference type="KEGG" id="nph:NP_3590A"/>
    <dbReference type="eggNOG" id="arCOG00684">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_0_1_2"/>
    <dbReference type="OrthoDB" id="33505at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021027">
        <property type="entry name" value="Transposase_put_HTH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF11">
        <property type="entry name" value="RNA-GUIDED DNA ENDONUCLEASE RV2885C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12323">
        <property type="entry name" value="HTH_OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Transposase putative helix-turn-helix" evidence="11">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="9">
        <location>
            <begin position="191"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="10">
        <location>
            <begin position="303"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="215"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI49886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="413" mass="47924" checksum="39B38237A544DD37" modified="2017-05-10" version="1">MEHSHRYHAYPTQEVAERLERHLDVHRQLYNHIRWDYEQAPEDDKPSEYDQNNKLPGWKRKWPVFSELHSKAAQATVARFHRNLSNLRKKKEKGYNVGRLKRQAPTDYRSVTYNQSGFDLDEKRGHDRYAHVRFSKIGWVKIRYHRPIPDHATIKEVTFKKETTGEWFVSFGLETDDADLPEKPPVDSLDASNSVGIDLGILNYIHTSDGKTVDWLDLEDEYERLRREQRKLSRKERGSSNYEKQRLKVAKVKRHIKRKVLDYQHKITTWLVKEYAAVFVEELNVKGMLEQSHNARNKQDAAWRQFITLLEYKADLYGCHVVQVEARGTTKECASCGVETAKPIWVRKHSCPSCGFETDRDANAAMNVLQRGFSELGLGWPEDTPVETVTATDTPEFQCVSASHVVETGSLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXP0</accession>
    <name>A0A1U7EXP0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Peroxisomal trans-2-enoyl-CoA reductase</fullName>
            <ecNumber evidence="13">1.3.1.38</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ordered locus">NP_3702A</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Participates in chain elongation of fatty acids. Catalyzes the reduction of trans-2-enoyl-CoAs of varying chain lengths from 6:1 to 16:1, having maximum activity with 10:1 CoA. Has no 2,4-dienoyl-CoA reductase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>(2E)-decenoyl-CoA + NADPH + H(+) = decanoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:44960"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:61406"/>
            <dbReference type="ChEBI" id="CHEBI:61430"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:44961"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(2E)-dodecenoyl-CoA + NADPH + H(+) = dodecanoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:44964"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57330"/>
            <dbReference type="ChEBI" id="CHEBI:57375"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:44965"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(2E)-hexenoyl-CoA + NADPH + H(+) = hexanoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:44956"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:62077"/>
            <dbReference type="ChEBI" id="CHEBI:62620"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:44957"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>(2E)-octenoyl-CoA + NADPH + H(+) = octanoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:44952"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57386"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:62242"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:44953"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(2E)-tetradecenoyl-CoA + NADPH + H(+) = tetradecanoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:44968"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57385"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:61405"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:44969"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a (2E)-enoyl-CoA + NADPH + H(+) = a 2,3-saturated acyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:33763"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58856"/>
            <dbReference type="ChEBI" id="CHEBI:65111"/>
            <dbReference type="EC" id="1.3.1.38"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:33764"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with PEX5, probably required to target it into peroxisomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.3.1.38" evidence="13"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323560.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXP0"/>
    <dbReference type="STRING" id="348780.NP_3702A"/>
    <dbReference type="EnsemblBacteria" id="CAI49942">
        <property type="protein sequence ID" value="CAI49942"/>
        <property type="gene ID" value="NP_3702A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701843"/>
    <dbReference type="KEGG" id="nph:NP_3702A"/>
    <dbReference type="eggNOG" id="arCOG01259">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_2_2"/>
    <dbReference type="OrthoDB" id="7442at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019166">
        <property type="term" value="F:trans-2-enoyl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05369">
        <property type="entry name" value="TER_DECR_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052388">
        <property type="entry name" value="Peroxisomal_t2-enoyl-CoA_red"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24317">
        <property type="entry name" value="PEROXISOMAL TRANS-2-ENOYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24317:SF7">
        <property type="entry name" value="PEROXISOMAL TRANS-2-ENOYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="7 21" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00041063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00049559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAI49942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="269" mass="27617" checksum="689A5EB7F29AE2B7" modified="2017-05-10" version="1">MTDEWPQTPPTTELFADDLLAGETALITGGGTGIGKAMAVAFAEHGADVAVASRDMDHLRPVVEAAEDAGADAFATTVDIRKPEAVEEMTATVLDELGDISILVNNAGANFVTPFEELSPNGWRAVVGTILDGTAYCTMTVGEHLIERGGGVVLSMGATNSVHGAPFHAHSGAGKAGVHNLMQTVASEWADHGVRANTIAPGIIETEGVTEAAGGALPPELLEDIPADRFGTPADCVSLALFLASPAAAYVTGGYYPVDGGQLLFSSPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXP8</accession>
    <name>A0A1U7EXP8_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">D-lactate dehydrogenase (cytochrome)</fullName>
            <ecNumber evidence="7">1.1.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">NP_3764A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAD-binding oxidoreductase/transferase type 4 family.</text>
    </comment>
    <dbReference type="EC" id="1.1.2.4" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49973.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939630.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXP8"/>
    <dbReference type="STRING" id="348780.NP_3764A"/>
    <dbReference type="EnsemblBacteria" id="CAI49973">
        <property type="protein sequence ID" value="CAI49973"/>
        <property type="gene ID" value="NP_3764A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702666"/>
    <dbReference type="KEGG" id="nph:NP_3764A"/>
    <dbReference type="eggNOG" id="arCOG00337">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017779_9_2_2"/>
    <dbReference type="OrthoDB" id="26910at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004458">
        <property type="term" value="F:D-lactate dehydrogenase (cytochrome) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008720">
        <property type="term" value="F:D-lactate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903457">
        <property type="term" value="P:lactate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.45.10:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2740:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="entry name" value="Vanillyl-alcohol Oxidase, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-bd_oxidored_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748:SF111">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="9">
        <location>
            <begin position="38"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="453"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI49973.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="473" mass="50470" checksum="D17A823A08EA9DF5" modified="2017-05-10" version="1">MPHDCSFLDGLALEGDVSFNEGERENHAADWVAEEAGFGVTPDAVVWPASTADVSAVLAAANEHGVPVTPYAAGTSLEGNAVPLFRGISMDMTKLDSVLAVRPDDFQIDVEPGVMGSAVDAAAADHGLFFPPMPSSGNLSTIGGMIANDASGQKTVRYGEVADWILELEVVLADGTVIETGSKAVKTSSGYNLKDLIIGSEGTLGVVTRATLELEGRPEQIQGGRAVFASVDAAAEAVSDVVQSGVDVAKLELLDPLAAEMANEYFDTGLPEGAMIFFEFHANHGIDEEVAFFRTVLEAHDVTEFEIADEDRMDELWRARDELAFAIQQYDPELSPRHPGDVTVPLSDYPEMVRYIRDLGEEYDVLLPCFGHAGDGNIHYTVLVDQDDGAMVETAEEIYDAIVERALEHGGTATGEHGIGMGKRRFLEAEHGEGGVAAMRAVKAALDPTDILNPGKIFPETEPEGGRVNLPGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXU7</accession>
    <name>A0A1U7EXU7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3890A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50036.3"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323653.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXU7"/>
    <dbReference type="STRING" id="348780.NP_3890A"/>
    <dbReference type="EnsemblBacteria" id="CAI50036">
        <property type="protein sequence ID" value="CAI50036"/>
        <property type="gene ID" value="NP_3890A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701905"/>
    <dbReference type="KEGG" id="nph:NP_3890A"/>
    <dbReference type="eggNOG" id="arCOG07565">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1412327_0_0_2"/>
    <dbReference type="OrthoDB" id="222031at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50036.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="175" mass="20191" checksum="9EECE859EFAD25F3" modified="2017-05-10" version="1">MSNDSKRLDELEGQLNALQQGHDNAWDAIEDLQDELQEVRTEQRRLQEDQDDLHDAVDHIDSRTDLLRLVENSDEMSGKQRSVALIQHLRRAAMRERERGRAAKVSINREEAERALQYPDVDRTTIYTDMDRAERLVGDKEILWYESGSGGDSRLKLNLEVGELPTKLTQEHGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY72</accession>
    <name>A0A1U7EY72_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Probable branched-chain amino acid transport ATP-binding protein LivG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">livG1</name>
        <name evidence="10" type="synonym">abc05a2</name>
        <name evidence="10" type="ordered locus">NP_4136A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Probable component of a branched-chain amino-acid transport system.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323775.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY72"/>
    <dbReference type="STRING" id="348780.NP_4136A"/>
    <dbReference type="EnsemblBacteria" id="CAI50159">
        <property type="protein sequence ID" value="CAI50159"/>
        <property type="gene ID" value="NP_4136A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702378"/>
    <dbReference type="KEGG" id="nph:NP_4136A"/>
    <dbReference type="eggNOG" id="arCOG00925">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OrthoDB" id="44250at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03219">
        <property type="entry name" value="ABC_Mj1267_LivG_branched"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000421">
        <property type="entry name" value="Branched-chain amino acid ABC transporter ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051120">
        <property type="entry name" value="ABC_AA/LPS_Transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032823">
        <property type="entry name" value="BCA_ABC_TP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45772:SF9">
        <property type="entry name" value="CONSERVED COMPONENT OF ABC TRANSPORTER FOR NATURAL AMINO ACIDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45772">
        <property type="entry name" value="CONSERVED COMPONENT OF ABC TRANSPORTER FOR NATURAL AMINO ACIDS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12399">
        <property type="entry name" value="BCA_ABC_TP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="4 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="34"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00056071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00072811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI50159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="285" mass="31427" checksum="A536C52DFF8905A1" modified="2017-05-10" version="1">MSDAPTAAPPEELGEVEDSPTEQAAQETPPGLPLRVNNLKKEFGGITAVDGASFQVEEGTLTGLIGPNGAGKSTTFNCITGVHKATSGHIHFKDEEITGLEPYEIAQKGLVRTFQIARELEEMTVLENLMLAPKNQRGEAIWRSVTPGARRGVIAEEKDVRKRAWETLEFFEIDHLAHEYAGGLSGGQRKLLEMARALMTDPEMVLLDEPLAGVNPTLEEKILDRIHELREQGQTFLLVEHDMDVIMNNCEHIIVMHQGQVLAEGTAEDIRDNDQVIEAYLGEDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYE3</accession>
    <name>A0A1U7EYE3_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Transcription initiation factor IIB</fullName>
            <shortName evidence="10">TFIIB</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">tfbA5</name>
        <name evidence="10" type="synonym">tfb</name>
        <name evidence="14" type="ordered locus">NP_4326A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre-initiation complex (DNA-TBP-TFIIB).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the TFIIB family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323870.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYE3"/>
    <dbReference type="STRING" id="348780.NP_4326A"/>
    <dbReference type="EnsemblBacteria" id="CAI50254">
        <property type="protein sequence ID" value="CAI50254"/>
        <property type="gene ID" value="NP_4326A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703237"/>
    <dbReference type="KEGG" id="nph:NP_4326A"/>
    <dbReference type="eggNOG" id="arCOG01981">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043736_0_0_2"/>
    <dbReference type="OrthoDB" id="7429at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20550">
        <property type="entry name" value="CYCLIN_TFIIB_archaea_like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000023">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.170:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00383">
        <property type="entry name" value="TF2B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023484">
        <property type="entry name" value="TFIIB_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023486">
        <property type="entry name" value="TFIIB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001658">
        <property type="entry name" value="PRK00423.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF13">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
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            <begin position="9"/>
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    <feature type="binding site" evidence="10">
        <location>
            <position position="33"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
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        <location>
            <position position="36"/>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:29105"/>
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        <location>
            <position position="51"/>
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        <location>
            <position position="54"/>
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        <ligand>
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            <dbReference type="PROSITE" id="PS51134"/>
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            <dbReference type="EMBL" id="CAI50254.1"/>
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    <sequence length="327" mass="36738" checksum="18AA1A33D56974F0" modified="2017-05-10" version="1">MSDTGIRTYTEKSRTETTEQTDETERNEGVATCPECDARLQADTEHGETVCSDCGLVVEEDEIDRGPEWRAFDSAEKDEKSRVGAPTTNMMHDKGLSTNIGWQDKDAYGNSLSSRQRQKMQRLRTWNERFRTRDSKERNLKQALGEIERMASALGLPENVRETASVIYRRALSEDLLPGRSIEGVATAALYAAARQANVPRSLDEVEHVSRVDRMELTRTYRYIIRELNLEVQPADPESYIPRFVSDLDRSDEVERRARQLIDNAREAGVISGKSPVGLAAAAVYAASLLCNEKVTQTEVSEVAEISEVTIRNRYKELLEASEVATA</sequence>
</entry>
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    <accession>A0A1U7EYE6</accession>
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        </submittedName>
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        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
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            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
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        <source>
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        <source>
            <dbReference type="EMBL" id="CAI50238.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="181" mass="20660" checksum="56BAEF62294BE932" modified="2017-05-10" version="1">MSDPLDEPEGNWWDEKVNRRETMWLGIAGLWSLGIFGWMSGFTRFGDQNPVGETYEVTPEEFQAAVDEYKEAADETEDGHLIPPGDSVYIAGRRFDWDGLPVVLEAGREYDIHLGAYDVQHGFSVRPEETLSKQINLQIFPDHEWVIPMTFEEPGTYQVICNEFCGRGHNGMQGKFIVEEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYF5</accession>
    <name>A0A1U7EYF5_NATPD</name>
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        <submittedName>
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        <name evidence="9" type="ordered locus">NP_4320A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
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        </lineage>
    </organism>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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            <authorList>
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                <person name="Pfeiffer F."/>
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                <person name="Rodewald K."/>
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                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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    </dbReference>
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    <dbReference type="STRING" id="348780.NP_4320A"/>
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        <property type="gene ID" value="NP_4320A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702030"/>
    <dbReference type="KEGG" id="nph:NP_4320A"/>
    <dbReference type="eggNOG" id="arCOG04203">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036781_1_1_2"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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    <dbReference type="InterPro" id="IPR003416">
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    <dbReference type="InterPro" id="IPR049177">
        <property type="entry name" value="MgtC_SapB_SrpB_YhiD_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39084:SF1">
        <property type="entry name" value="DUF4010 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39084">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13194">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02308">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01837">
        <property type="entry name" value="MGTCSAPBPROT"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="368"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="398"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="MgtC/SapB/SrpB/YhiD N-terminal" evidence="7">
        <location>
            <begin position="16"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4010" evidence="8">
        <location>
            <begin position="185"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI50251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="420" mass="42432" checksum="B29D5A1ECB6A42CC" modified="2017-05-10" version="1">MSELGPAMLDDNVAALVLAALLGLFLGLEREWSDKVAGIRTFALVSLIAAVFTILGEPLLLFVGAAFVVVVGVILGIRGLVGQTETLSLTTSASLLVAYGVGSLVASGHVLEGVTVTVVSSLLLVLKQELHAVAGSLSRQEVRSSTEFAILAFVIYPLLPPEERTIAVGDAAVAVEPRVVWLMVVFVAGIGIANYAIVQLYGGRGIAVTGFFGGFASSTGVVGAMLDHVSQQPDASRYGIAAVVLSNAAMAMRNLAIAVVFTIGAGVLVEAVIPLGAVVVGAIVVAAFTAEWSESVEMDLGAPFTLRYALGVGGLFLVVLLAGGFAEALFGTEGLYAAAAISGLVSSAGATASAVILYSNGQITADQAVVAILLASAASIAVKAGLSALSPNREFARGVVVWSAVLLVGAGAVTGLVVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYG0</accession>
    <name>A0A1U7EYG0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycerophosphodiester phosphodiesterase</fullName>
            <ecNumber evidence="2">3.1.4.46</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">glpQ</name>
        <name evidence="2" type="synonym">ugpQ</name>
        <name evidence="2" type="ordered locus">NP_4360A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.4.46" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323887.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYG0"/>
    <dbReference type="STRING" id="348780.NP_4360A"/>
    <dbReference type="EnsemblBacteria" id="CAI50271">
        <property type="protein sequence ID" value="CAI50271"/>
        <property type="gene ID" value="NP_4360A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703444"/>
    <dbReference type="KEGG" id="nph:NP_4360A"/>
    <dbReference type="eggNOG" id="arCOG00701">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030006_3_4_2"/>
    <dbReference type="OrthoDB" id="19020at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008889">
        <property type="term" value="F:glycerophosphodiester phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08556">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46211:SF14">
        <property type="entry name" value="GLYCEROPHOSPHODIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46211">
        <property type="entry name" value="GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GP-PDE" evidence="1">
        <location>
            <begin position="1"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="226" mass="23648" checksum="E79F1F9B07E059F9" modified="2017-05-10" version="1">MRLIAHRGFAATAPENTLQAVTEAAEVADGVEVDVRRCGSGELVVIHDETVDRVTDASGPVSSFTRTDLDALDVLDTGEGVPTLAAVLEAVPAHIGITLDLKESGIAADAVALLSETRQQVTLSSRSREVLAACRDAAPELPRAYITDGSDESGTDAVEVAIDLDCAFLHPSLDVATERVVAEAHRAGMSVNAWTVDTPADAETLAERGVDGVIADRPVSLPESRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYI5</accession>
    <name>A0A1U7EYI5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PadR family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4444A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323928.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYI5"/>
    <dbReference type="EnsemblBacteria" id="CAI50313">
        <property type="protein sequence ID" value="CAI50313"/>
        <property type="gene ID" value="NP_4444A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702065"/>
    <dbReference type="KEGG" id="nph:NP_4444A"/>
    <dbReference type="eggNOG" id="arCOG00008">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130789_0_0_2"/>
    <dbReference type="OrthoDB" id="56053at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator PadR N-terminal" evidence="1">
        <location>
            <begin position="69"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="163" mass="17830" checksum="447080B865FADAD9" modified="2017-05-10" version="1">MSSQGNVEPTLERLVTELGDGDATRDDADPAARTEAVVTEASETLFEGGTLHVDDGLIKQSLPELLLLLVGLRSTDTHGKGIMEDLSRFFGAQLSPGTVYPMLHDLEADDLLEMRELVQTKEYTIDDTEAVRGEIQAAMQQHLALGLVFSRALDEIDVDEMDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYM6</accession>
    <name>A0A1U7EYM6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Mechanosensitive channel protein MscS</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">mscS7</name>
        <name evidence="10" type="ordered locus">NP_4476A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323944.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYM6"/>
    <dbReference type="STRING" id="348780.NP_4476A"/>
    <dbReference type="EnsemblBacteria" id="CAI50329">
        <property type="protein sequence ID" value="CAI50329"/>
        <property type="gene ID" value="NP_4476A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702944"/>
    <dbReference type="KEGG" id="nph:NP_4476A"/>
    <dbReference type="eggNOG" id="arCOG01568">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037945_1_0_2"/>
    <dbReference type="OrthoDB" id="31543at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049278">
        <property type="entry name" value="MS_channel_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045275">
        <property type="entry name" value="MscS_archaea/bacteria_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_beta-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011066">
        <property type="entry name" value="MscS_channel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011014">
        <property type="entry name" value="MscS_channel_TM-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30221">
        <property type="entry name" value="SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30221:SF1">
        <property type="entry name" value="SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21082">
        <property type="entry name" value="MS_channel_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82689">
        <property type="entry name" value="Mechanosensitive channel protein MscS (YggB), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82861">
        <property type="entry name" value="Mechanosensitive channel protein MscS (YggB), transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS" evidence="8">
        <location>
            <begin position="123"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS C-terminal" evidence="9">
        <location>
            <begin position="197"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI50329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="302" mass="33222" checksum="07B8EF30238870A5" modified="2017-05-10" version="1">MSGGLLQTGAGRFIADTLETVGVPPELAGPFGDTIVGVSLFGIAFILLYVVGKRFILPLLNRVLNARDLDRHAKTPIRKLVWLVYLFVGITIAFGVAGYDDFLRSLATIAAAATLAIGFAMQDVLKNFVAGIFIFTERPFKIGDWIEWDDNTGVVEDISLRVTRVRTFDNELLTVPNSQLTDNVVKNPVDADKLRLKFVFGIGYDDDIDAATDIILEEARAHDGIMDAPEPSVRLTELGDSDVGLQSRIWIASPSRADFVKIRGEYVKRVKERFDEEGIDIPYPHRTLTGGIEVADGPVAEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYN5</accession>
    <name>A0A1U7EYN5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HTH domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4508A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50345.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_193763572.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYN5"/>
    <dbReference type="STRING" id="348780.NP_4508A"/>
    <dbReference type="EnsemblBacteria" id="CAI50345">
        <property type="protein sequence ID" value="CAI50345"/>
        <property type="gene ID" value="NP_4508A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702082"/>
    <dbReference type="KEGG" id="nph:NP_4508A"/>
    <dbReference type="eggNOG" id="arCOG06369">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2662243_0_0_2"/>
    <dbReference type="OrthoDB" id="210390at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50345.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="76" mass="8634" checksum="0A8A75005D396FE2" modified="2017-05-10" version="1">MRIRRGEFERIRTVLAEADPDEPLTAREILDLLDEHGESFDSAHRVATVLGREADAGPIEVVDGQPYRYRLGEEAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYP7</accession>
    <name>A0A1U7EYP7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4522A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323967.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYP7"/>
    <dbReference type="STRING" id="348780.NP_4522A"/>
    <dbReference type="EnsemblBacteria" id="CAI50352">
        <property type="protein sequence ID" value="CAI50352"/>
        <property type="gene ID" value="NP_4522A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702088"/>
    <dbReference type="KEGG" id="nph:NP_4522A"/>
    <dbReference type="eggNOG" id="arCOG06389">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_157003_0_0_2"/>
    <dbReference type="OrthoDB" id="166257at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="107" mass="11992" checksum="AA8E824198DDFE19" modified="2017-05-10" version="1">MATNASTSGSLTDGPAKTALPSEVPYRTRLSWELGTRVIEDEATERRSHWEGSDGTWELTVFDATTTTLVMRVRTPVGRERFYGAEMSMLSSIHARLEADVDWQRAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYV3</accession>
    <name>A0A1U7EYV3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cupin 2 barrel domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4718A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324064.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYV3"/>
    <dbReference type="EnsemblBacteria" id="CAI50450">
        <property type="protein sequence ID" value="CAI50450"/>
        <property type="gene ID" value="NP_4718A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702156"/>
    <dbReference type="KEGG" id="nph:NP_4718A"/>
    <dbReference type="eggNOG" id="arCOG03008">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149791_1_0_2"/>
    <dbReference type="OrthoDB" id="199885at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin type-2" evidence="1">
        <location>
            <begin position="43"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="105" mass="11465" checksum="D4F3D1C81D3330D4" modified="2017-05-10" version="1">MKHLTAEELADQNVLQEGDITTVFDEAAMVVEQSRYQAETAAAESPRTHADDEFYYITAGAGKMRVGDETSEVSAGDVVYVDGGVKHDFFDIEGELRTLKVFASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYY1</accession>
    <name>A0A1U7EYY1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">IS1341-type transposase ISNph11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">NP_4782A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324094.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYY1"/>
    <dbReference type="STRING" id="348780.NP_4782A"/>
    <dbReference type="EnsemblBacteria" id="CAI50482">
        <property type="protein sequence ID" value="CAI50482"/>
        <property type="gene ID" value="NP_4782A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702173"/>
    <dbReference type="KEGG" id="nph:NP_4782A"/>
    <dbReference type="eggNOG" id="arCOG00684">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_0_1_2"/>
    <dbReference type="OrthoDB" id="33505at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021027">
        <property type="entry name" value="Transposase_put_HTH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF11">
        <property type="entry name" value="RNA-GUIDED DNA ENDONUCLEASE RV2885C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12323">
        <property type="entry name" value="HTH_OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Transposase putative helix-turn-helix" evidence="11">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="9">
        <location>
            <begin position="189"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="10">
        <location>
            <begin position="303"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="215"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI50482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="413" mass="48002" checksum="E31886525E0C3CC3" modified="2017-05-10" version="1">MEHSHRYRAYPTQEVAERLEHHLDVHRQLYNHVRWDYENSPEDDKPSEYDQNNKLPEWKRKWPVFGELHSKAAQATVARFHRNLSNLRKKKEKGYNVGQLKRQAPTEYRSMTYNQSGFDLDEKRGRDRFAYVRFSKIGWVKIRYHRPIPDFASIKEVTVKKETTGEWFVSFGLETDDADLPEKPDVNSLDASNSVGIDLGIQNYIHTSDGKTVDWLNLEDEYERLRRQQRKLSRKEEGSNNYEKQRREVAKVKRHIKRKVLDYQHKITTWLVREYDAVFVEDLDVKGMLEQSHNGRNKQDAAWRQFITLLEYKADLYGCHVVQVEARGTTKECASCGVESAKPIWVREHSCPSCGFETDRDANAAMNVLERGFSALGLGWPEDTPVETVTATDTDDIHEVSASHVVETGSLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ01</accession>
    <name>A0A1U7EZ01_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4824A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ01"/>
    <dbReference type="STRING" id="348780.NP_4824A"/>
    <dbReference type="EnsemblBacteria" id="CAI50503">
        <property type="protein sequence ID" value="CAI50503"/>
        <property type="gene ID" value="NP_4824A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_4824A"/>
    <dbReference type="HOGENOM" id="CLU_2629836_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="77" mass="8382" checksum="9A7008B10DFC6CAA" modified="2017-05-10" version="1">MYDARTRAAVARYVRGLSTAREAAREADISTAELRHYARTCGIAPAFTGDEHVDSADSERAAGTESERDDDTKCSND</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ02</accession>
    <name>A0A1U7EZ02_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Amidohydrolase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4788A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324097.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ02"/>
    <dbReference type="STRING" id="348780.NP_4788A"/>
    <dbReference type="EnsemblBacteria" id="CAI50485">
        <property type="protein sequence ID" value="CAI50485"/>
        <property type="gene ID" value="NP_4788A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702788"/>
    <dbReference type="KEGG" id="nph:NP_4788A"/>
    <dbReference type="eggNOG" id="arCOG00692">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012358_1_0_2"/>
    <dbReference type="OrthoDB" id="42910at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050287">
        <property type="entry name" value="MTA/SAH_deaminase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43794">
        <property type="entry name" value="AMINOHYDROLASE SSNA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43794:SF5">
        <property type="entry name" value="CHLOROHYDROLASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="1">
        <location>
            <begin position="44"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="341" mass="36308" checksum="4850F05A5F3AA57B" modified="2017-05-10" version="1">METLEGTVLAGRSFDPMYGRVVIEEGHITAVERIDDHDGDASRIILPAFVNAHSHVGDSVAKEAAVDLGLEAAVAPPDSLKHQRLQAADRETAVDAMYRTLRFMQQTGTAAFLDFREFGIEGAKTLREAAEGLDIEAFIFGSDDAAVLDVADGFGASGANDDDFTSQRAAAAARGVPFAIHAGEPDATDIHPALDLEPDLLVHMVHAEAEHLERVADQETPIAVCPRANRVLGVGRPPIETLLEYTDVALGTDNVMLNPPSMFREMAYTAKTFELPSQTVLRMATTAGAEAVGLDCGVIEPGRRAALLVLDGDSDNLSGVADPVDAVVRRATALDVERVIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ42</accession>
    <name>A0A1U7EZ42_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Trk-type transport system (Probable substrate potassium)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">trkH2</name>
        <name evidence="13" type="synonym">tp29u2b</name>
        <name evidence="13" type="ordered locus">NP_4906A</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TrkH potassium transport family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324156.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ42"/>
    <dbReference type="STRING" id="348780.NP_4906A"/>
    <dbReference type="EnsemblBacteria" id="CAI50544">
        <property type="protein sequence ID" value="CAI50544"/>
        <property type="gene ID" value="NP_4906A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703306"/>
    <dbReference type="KEGG" id="nph:NP_4906A"/>
    <dbReference type="eggNOG" id="arCOG04145">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030708_0_2_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015379">
        <property type="term" value="F:potassium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003445">
        <property type="entry name" value="Cat_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004772">
        <property type="entry name" value="TrkH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32024">
        <property type="entry name" value="TRK SYSTEM POTASSIUM UPTAKE PROTEIN TRKG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32024:SF2">
        <property type="entry name" value="TRK SYSTEM POTASSIUM UPTAKE PROTEIN TRKG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02386">
        <property type="entry name" value="TrkH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006247">
        <property type="entry name" value="TrkH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="246"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="290"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="420"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="452"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="487"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAI50544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="514" mass="54948" checksum="8AD60514CD837D1A" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ44</accession>
    <name>A0A1U7EZ44_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Probable anaerobic dehydrogenase iron-sulfur-binding subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">NP_4960A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
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        <property type="entry name" value="ET_IronSulfur_Cluster-Binding"/>
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        <property type="entry name" value="PROTEIN NRFC"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PROTEIN NRFC HOMOLOG"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Fer4_11"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="10"/>
            <end position="38"/>
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    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="52"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="84"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI50571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="199" mass="21493" checksum="4F857A97AF715995" modified="2017-05-10" version="1">MSTNQSNDTARVIPNWESCIDCGACEVACQRTWDLPPETNRIEVVALEHGTEDEAQKPMQCYNCAEAPCVDVCPTDALLFRDDGTVRVSGDLCIGCHYCGVGCPFGAPQYPDAEVPEADEMNPLGARGNGLMDKCTTCEPRQEEDLEPACSSECPTDALLFGTPDELSEKLDEEGTSTPFEPEAARIVFGDDADSVISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ55</accession>
    <name>A0A1U7EZ55_NATPD</name>
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        <submittedName>
            <fullName evidence="12">Probable anaerobic dehydrogenase alpha subunit</fullName>
        </submittedName>
    </protein>
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        <name evidence="12" type="ordered locus">NP_4962A</name>
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    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
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            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
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    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.</text>
    </comment>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:anaerobic respiration"/>
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        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
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        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
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        <property type="entry name" value="Mopterin_OxRdtase"/>
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    </dbReference>
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        <property type="entry name" value="TAT_signal_bac_arc"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FORMATE DEHYDROGENASE-O MAJOR SUBUNIT"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR43598">
        <property type="entry name" value="TUNGSTEN-CONTAINING FORMYLMETHANOFURAN DEHYDROGENASE 2 SUBUNIT B"/>
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    </dbReference>
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        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Molybdopterin"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FDH_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00926">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF53706">
        <property type="entry name" value="Formate dehydrogenase/DMSO reductase, domains 1-3"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00551">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="11">
        <location>
            <begin position="52"/>
            <end position="108"/>
        </location>
    </feature>
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        <location>
            <begin position="956"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="970"/>
            <end position="979"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI50572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="979" mass="109247" checksum="0028769674DA5B6D" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ76</accession>
    <name>A0A1U7EZ76_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7124 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_4966A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324186.1">
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ76"/>
    <dbReference type="STRING" id="348780.NP_4966A"/>
    <dbReference type="EnsemblBacteria" id="CAI50574">
        <property type="protein sequence ID" value="CAI50574"/>
        <property type="gene ID" value="NP_4966A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702214"/>
    <dbReference type="KEGG" id="nph:NP_4966A"/>
    <dbReference type="eggNOG" id="arCOG03064">
        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_094457_0_0_2"/>
    <dbReference type="OrthoDB" id="221805at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055548">
        <property type="entry name" value="DUF7124"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23439">
        <property type="entry name" value="DUF7124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7124" evidence="2">
        <location>
            <begin position="120"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="238" mass="25384" checksum="0C2C517CD3BB40DE" modified="2017-05-10" version="1">MTERIDLDDMAVEEKAEDPDGNYGDWLWRGEGDPDDEPSPGWTDGDTEATSESEESTGGGPDRQAAPGVPSTGGGGPVGVPEDRGGAGGGTSGSAAENTPGGGIEQAGTAETHDVDIDDMTMALTYKAAHHFTEPQRVFAEAREWADWVGVVGEVSTPAIRKFQREAGVELDFFGGSESGPDERLADIDAESMFYAERMVVVGVDGDEWIADAADWEFVPLEHAAEKADWELKEEFLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ84</accession>
    <name>A0A1U7EZ84_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">IS1341-type transposase ISNph13</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">NP_5054A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324228.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ84"/>
    <dbReference type="STRING" id="348780.NP_5054A"/>
    <dbReference type="EnsemblBacteria" id="CAI50618">
        <property type="protein sequence ID" value="CAI50618"/>
        <property type="gene ID" value="NP_5054A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702241"/>
    <dbReference type="KEGG" id="nph:NP_5054A"/>
    <dbReference type="eggNOG" id="arCOG00684">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_0_1_2"/>
    <dbReference type="OrthoDB" id="33505at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021027">
        <property type="entry name" value="Transposase_put_HTH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12323">
        <property type="entry name" value="HTH_OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0815">Transposition</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Transposase putative helix-turn-helix" evidence="9">
        <location>
            <begin position="6"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="7">
        <location>
            <begin position="189"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="8">
        <location>
            <begin position="307"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI50618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="426" mass="48990" checksum="6FE98032C1A9B60E" modified="2017-05-10" version="1">MDYSPRFRLLPTSRQRELLGWQRDTVRQVYNHALYRLDQLPERADQTVRQRVWSVRDELPDWKTKWEEWSSVYSTVLQAAVERIYHSITGLAQQRDNGHKIGDLNWKKPREFQSFTYRQRGFELDNKSGPEGRGLLTLKKVRGKTLEIPIRIHRDIDPDDIKHVTVKQEATGAWYTSFSIDRDEPEKPDPEDIDPEDTVGLDLGVLNFIHDSDGRSINRLDLSDDRERLEREHRSLSRKEYESNNWEKQRQRVAEVHMDMRNKKRDYKHKLAHAYTTEYDAVFVEDLDVKPMLEDDGNARNKAEVGWRDFIRILKHHGRKHGCHVVEVEPAGTTKECASCGVETAKPLWVREHSCPACGFELGRDFNASLNVLSRGLAKLGVGHSEATTPAETATAVDSVSVSASRVVESGSPCLKEPAKQASRQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ88</accession>
    <name>A0A1U7EZ88_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UspA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_5024A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324213.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ88"/>
    <dbReference type="STRING" id="348780.NP_5024A"/>
    <dbReference type="EnsemblBacteria" id="CAI50603">
        <property type="protein sequence ID" value="CAI50603"/>
        <property type="gene ID" value="NP_5024A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702231"/>
    <dbReference type="KEGG" id="nph:NP_5024A"/>
    <dbReference type="eggNOG" id="arCOG02053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_11_4_2"/>
    <dbReference type="OrthoDB" id="105697at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd23659">
        <property type="entry name" value="USP_At3g01520-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF24">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="2"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="139" mass="14763" checksum="33A0691F9DD28C8D" modified="2017-05-10" version="1">MRVLVPMDGSEQSWTAFEHAADEFPEATLVCLRAIDPSKAGYGAGMEAGGVARVLDAERDAANDMLERAVERGDDRGVSVATAAEIGRPARVIIDATENEDIDHVVIGSHGRDGISRILLGSVAETVVRRSPVPVTVVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZB6</accession>
    <name>A0A1U7EZB6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cold shock protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cspA1</name>
        <name evidence="4" type="ordered locus">NP_5064A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324233.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZB6"/>
    <dbReference type="STRING" id="348780.NP_5064A"/>
    <dbReference type="EnsemblBacteria" id="CAI50623">
        <property type="protein sequence ID" value="CAI50623"/>
        <property type="gene ID" value="NP_5064A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702640"/>
    <dbReference type="KEGG" id="nph:NP_5064A"/>
    <dbReference type="eggNOG" id="arCOG02983">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117621_7_2_2"/>
    <dbReference type="OrthoDB" id="202422at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012156">
        <property type="entry name" value="Cold_shock_CspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050181">
        <property type="entry name" value="Cold_shock_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11544">
        <property type="entry name" value="COLD SHOCK DOMAIN CONTAINING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002599">
        <property type="entry name" value="Cold_shock_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00050">
        <property type="entry name" value="COLDSHOCK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CSD" evidence="3">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="64" mass="7092" checksum="82DC77D6FFA9ABC7" modified="2017-05-10" version="1">MATGTVDFFNDTGGYGFIETEDADEDVFFHMEDVGGPDLEEGQEVEFDIEEADKGPRATNLTRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZB7</accession>
    <name>A0A1U7EZB7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF8080 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5098A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324250.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZB7"/>
    <dbReference type="STRING" id="348780.NP_5098A"/>
    <dbReference type="EnsemblBacteria" id="CAI50640">
        <property type="protein sequence ID" value="CAI50640"/>
        <property type="gene ID" value="NP_5098A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702253"/>
    <dbReference type="KEGG" id="nph:NP_5098A"/>
    <dbReference type="eggNOG" id="arCOG06335">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1472135_0_0_2"/>
    <dbReference type="OrthoDB" id="193731at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057179">
        <property type="entry name" value="DUF7857"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058393">
        <property type="entry name" value="DUF8080"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25256">
        <property type="entry name" value="DUF7857"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26296">
        <property type="entry name" value="DUF8080"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8080" evidence="1">
        <location>
            <begin position="94"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="183" mass="19263" checksum="094F16AE575E4F7C" modified="2017-05-10" version="1">MPSIDAETRHIDGVTFVEGVVRADAPRRVQLVARCDGPVWPPRREGIPTENWDGDRVTVEAGPAPVGIGFATPGDVSTEPVAIVASEPVADELPTGVAAWVQNVESRLEAGERLAAAESLDEAANAVAAVGGLSAAEQLTDALARDRRLASQLSIVPPAVSERLETVDIPVEAFRTLAQSRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZE0</accession>
    <name>A0A1U7EZE0_NATPD</name>
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        <submittedName>
            <fullName evidence="2">Ribonuclease H domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5114A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324258.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZE0"/>
    <dbReference type="STRING" id="348780.NP_5114A"/>
    <dbReference type="EnsemblBacteria" id="CAI50648">
        <property type="protein sequence ID" value="CAI50648"/>
        <property type="gene ID" value="NP_5114A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702261"/>
    <dbReference type="KEGG" id="nph:NP_5114A"/>
    <dbReference type="eggNOG" id="arCOG03130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073770_0_0_2"/>
    <dbReference type="OrthoDB" id="211024at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038720">
        <property type="entry name" value="YprB_RNase_H-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38462">
        <property type="entry name" value="EXONUCLEASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38462:SF1">
        <property type="entry name" value="YPRB RIBONUCLEASE H-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13482">
        <property type="entry name" value="RNase_H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YprB ribonuclease H-like" evidence="1">
        <location>
            <begin position="85"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="243" mass="27359" checksum="6B81D330FDCA0A99" modified="2017-05-10" version="1">MRLENTYIAVDGVGETTERELWASGARTWSEFDPSLCGPTTADRIESFIEQARPRLDADDSAFFDRQLPSSERWRLYENFRDEACFFDIETTGLDQMRDTVTCVSIHQGGETETLVRGDDLTQGNLEALLDGPLLISFNGARFDVPFLETAFDIDIEAPHLDLMYPCKRVGLTGGLKAIEPEVGVERDRPDLSGKDAVRLWRDHERGVDGALETLISYNREDTVNLKTVADSAVERLDRKLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZG6</accession>
    <name>A0A1U7EZG6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RnhA operon protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_5190A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324296.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZG6"/>
    <dbReference type="STRING" id="348780.NP_5190A"/>
    <dbReference type="EnsemblBacteria" id="CAI50686">
        <property type="protein sequence ID" value="CAI50686"/>
        <property type="gene ID" value="NP_5190A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702287"/>
    <dbReference type="KEGG" id="nph:NP_5190A"/>
    <dbReference type="eggNOG" id="arCOG04769">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089552_0_0_2"/>
    <dbReference type="OrthoDB" id="203809at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056494">
        <property type="entry name" value="DUF7108_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055532">
        <property type="entry name" value="DUF7108_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23418">
        <property type="entry name" value="DUF7108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23420">
        <property type="entry name" value="DUF7108_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7108" evidence="1">
        <location>
            <begin position="5"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7108" evidence="2">
        <location>
            <begin position="95"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="183" mass="20789" checksum="8188A4B327C000A3" modified="2017-05-10" version="1">MAEDELPDTVVERAETLTRRARKAVDENEQTAYLEERGSLLSDHGYRARVREDDATEILVLYPDEWVEDGTVEPGEIDDTGRAVERRISGPASGDNWAEVDRHNRTVAGRVREEHGAVHGETAEAFATFMSNHYAKPVEEAAPDEKQEFRDEYFPRNAWPSDEQLAQVEASLEYIERVAERGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZH8</accession>
    <name>A0A1U7EZH8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_5208A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324305.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZH8"/>
    <dbReference type="STRING" id="348780.NP_5208A"/>
    <dbReference type="EnsemblBacteria" id="CAI50695">
        <property type="protein sequence ID" value="CAI50695"/>
        <property type="gene ID" value="NP_5208A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702295"/>
    <dbReference type="KEGG" id="nph:NP_5208A"/>
    <dbReference type="eggNOG" id="arCOG04673">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1615280_0_0_2"/>
    <dbReference type="OrthoDB" id="157493at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055707">
        <property type="entry name" value="DUF7283"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23954">
        <property type="entry name" value="DUF7283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="155" mass="16214" checksum="A8F37B887C37B236" modified="2017-05-10" version="1">MFDVPLDSWYVWFGLAVVSSATVGVAGTLPSSPPPDAAGGAQTIDSVAASDRPAVGKHPLSNAESVRIGTSSLSLRGGGEISHEALRYGPVTPAGADDRLQSVLRGSHPDKQFDTPAAFAEAAEAARTEQPQWYTTDELVVRRVIWEGTDVVLAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZK1</accession>
    <name>A0A1U7EZK1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HTH domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5330A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZK1"/>
    <dbReference type="EnsemblBacteria" id="CAI50756">
        <property type="protein sequence ID" value="CAI50756"/>
        <property type="gene ID" value="NP_5330A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_5330A"/>
    <dbReference type="eggNOG" id="arCOG08182">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2820737_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="Sigma3 and sigma4 domains of RNA polymerase sigma factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA polymerase sigma factor 70 region 4 type 2" evidence="1">
        <location>
            <begin position="18"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="77" mass="8680" checksum="78C0F1B1807A128C" modified="2017-05-10" version="1">MTQLSLTDFDSDGCDDGLTELTEAEREAYVAVEQDGVGVREFARETERRPGTVGNLLRRARMRLGEDDADQEVTETW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZL0</accession>
    <name>A0A1U7EZL0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">HTH-10 family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_5298A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324349.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZL0"/>
    <dbReference type="STRING" id="348780.NP_5298A"/>
    <dbReference type="EnsemblBacteria" id="CAI50740">
        <property type="protein sequence ID" value="CAI50740"/>
        <property type="gene ID" value="NP_5298A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702325"/>
    <dbReference type="KEGG" id="nph:NP_5298A"/>
    <dbReference type="eggNOG" id="arCOG02274">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096714_0_0_2"/>
    <dbReference type="OrthoDB" id="198846at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007050">
        <property type="entry name" value="HTH_bacterioopsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056529">
        <property type="entry name" value="HVO_2928_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236:SF1">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04967">
        <property type="entry name" value="HTH_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24281">
        <property type="entry name" value="HVO_2928_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HVO-2928 N-terminal" evidence="4">
        <location>
            <begin position="3"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="HTH bat-type" evidence="3">
        <location>
            <begin position="173"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI50740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="228" mass="25259" checksum="26E67AE70D85B6A0" modified="2017-05-10" version="1">MREVVFVVTHEPGVNPVADLLADHPAARLRSAACHVTESSLWRVDHAQGPAVVLDSLADMAGARYFTDCLVRDGCAGDWETTVLDRTDSSLVLYSYWERAPDCDSVPHLAREHFGEGVVIDETWRERSQRWRVLAPDGDISPFIEDVRAVADADVTFERVSDPDPAADDALPPEQADALAAAVEAGYYETPRETEAYDLADDLGIPGSTLTYRLRRAEAWLAKEYVDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZP1</accession>
    <name>A0A1U7EZP1_NATPD</name>
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        <submittedName>
            <fullName evidence="3">Homolog to virus structural protein HRPV2-VP5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_5348A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324373.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZP1"/>
    <dbReference type="STRING" id="348780.NP_5348A"/>
    <dbReference type="EnsemblBacteria" id="CAI50765">
        <property type="protein sequence ID" value="CAI50765"/>
        <property type="gene ID" value="NP_5348A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702344"/>
    <dbReference type="KEGG" id="nph:NP_5348A"/>
    <dbReference type="eggNOG" id="arCOG07782">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_445247_0_0_2"/>
    <dbReference type="OrthoDB" id="275660at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058677">
        <property type="entry name" value="ORF4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26255">
        <property type="entry name" value="Viral_env_HRPV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Envelope protein N-terminal" evidence="2">
        <location>
            <begin position="97"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="611" mass="66777" checksum="077622890020091D" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZQ5</accession>
    <name>A0A1U7EZQ5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3615D</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CDN71457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZQ5"/>
    <dbReference type="EnsemblBacteria" id="CDN71457">
        <property type="protein sequence ID" value="CDN71457"/>
        <property type="gene ID" value="NP_3615D"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3615D"/>
    <dbReference type="eggNOG" id="arCOG14362">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2581312_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDN71457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="80" mass="8930" checksum="0A9124DD0533C2B3" modified="2017-05-10" version="1">MRLRDPKEMLLIMAKITLALVGAIVMTALAILVLPFSDFSPQTPGRVVQVGVFYAAWALLIFRLVFGEYIPREAFKTSES</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I7L1Y1</accession>
    <name>I7L1Y1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Small CPxCG-related zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_6105F</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CCI69590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7L1Y1"/>
    <dbReference type="EnsemblBacteria" id="CCI69590">
        <property type="protein sequence ID" value="CCI69590"/>
        <property type="gene ID" value="NP_6105F"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_6105F"/>
    <dbReference type="HOGENOM" id="CLU_2646033_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025874">
        <property type="entry name" value="DZR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12773">
        <property type="entry name" value="DZR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DZANK-type" evidence="1">
        <location>
            <begin position="23"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCI69590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="76" mass="7649" checksum="4EAFC8C97490E283" modified="2012-10-03" version="1">MTPIPSLRGVLAALSGTDATVECPTCAASVDVSDSECSSCGAVLLVECRSCGETIGASTDACPACEKSDFGVFRID</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILV2</accession>
    <name>Q3ILV2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">UPF0395 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">NP_3280A</name>
        <name evidence="8" type="ordered locus">NP_7004A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8 9">
        <name>PL23</name>
    </geneLocation>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
            <strain evidence="8">Gabara</strain>
            <plasmid evidence="8">PL23</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CR936259">
        <property type="protein sequence ID" value="CAI50918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILV2"/>
    <dbReference type="STRING" id="348780.NP_3280A"/>
    <dbReference type="EnsemblBacteria" id="CAI49731">
        <property type="protein sequence ID" value="CAI49731"/>
        <property type="gene ID" value="NP_3280A"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI50918">
        <property type="protein sequence ID" value="CAI50918"/>
        <property type="gene ID" value="NP_7004A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3280A"/>
    <dbReference type="KEGG" id="nph:NP_7004A"/>
    <dbReference type="eggNOG" id="arCOG03087">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_164851_6_1_2"/>
    <dbReference type="OrthoDB" id="7619at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.920.30">
        <property type="entry name" value="Hypothetical protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012933">
        <property type="entry name" value="HicA_mRNA_interferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038570">
        <property type="entry name" value="HicA_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07927">
        <property type="entry name" value="HicA_toxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54786">
        <property type="entry name" value="YcfA/nrd intein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0346">Stress response</keyword>
    <keyword evidence="1" id="KW-1277">Toxin-antitoxin system</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI50918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="81" mass="8933" checksum="25FDBEB2479D89AE" modified="2005-11-08" version="1">MVRTSFSSRDVIKTLTKHGFVPVGGKGSHTTLRYENDETGEVRTVTVPKADPIPVGTLQQIAKQAGADDFHDFCQWVDRTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILW1</accession>
    <name>Q3ILW1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Homolog to carboxylate-amine ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_6258A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324506.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILW1"/>
    <dbReference type="EnsemblBacteria" id="CAI50909">
        <property type="protein sequence ID" value="CAI50909"/>
        <property type="gene ID" value="NP_6258A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694658"/>
    <dbReference type="KEGG" id="nph:NP_6258A"/>
    <dbReference type="HOGENOM" id="CLU_751435_0_0_2"/>
    <dbReference type="OrthoDB" id="156252at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004357">
        <property type="term" value="F:glutamate-cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042398">
        <property type="term" value="P:modified amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050141">
        <property type="entry name" value="GCL_type2/YbdK_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006336">
        <property type="entry name" value="GCS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36510">
        <property type="entry name" value="GLUTAMATE--CYSTEINE LIGASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36510:SF1">
        <property type="entry name" value="GLUTAMATE--CYSTEINE LIGASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04107">
        <property type="entry name" value="GCS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="387" mass="44082" checksum="B28FA24DAEA0FFB0" modified="2005-11-08" version="1">MSESPDTHRSPNRTTTTGSASEGEPIRRSIEVEYWVVDGEGRLTEPEDLVDASPGVEREFVAPLLEIKTTPCETTAQLREELFTRLERVLQRAKKQDKGLVPLGTPIYQGLIEDLPSERTRIQDRILGEDFEYVRHCAGTHIHVEQQPGRKIDQLNALIAIDPALALVNSSPYFEGTYLTTGARSQLYRWMAYNSLPHQGRLWSYIEDTAEWEARLERRYEEFVAEGVTAEVDRETTEACFDPESSVWTPVQLREEFSTVEWRSPDTALPSQIVRLADDIVDLIDHLRDAEVRIEGDTGRRTEDAIVLPEFSAVEEYVERAVRHGLSSDAVRQYLDRMGFNASSYAPLTHQIDAGEELTVAKTRELRLEYAERLEQDLSRARSTTVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM31</accession>
    <name>Q3IM31_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UspA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_6108A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324436.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM31"/>
    <dbReference type="EnsemblBacteria" id="CAI50834">
        <property type="protein sequence ID" value="CAI50834"/>
        <property type="gene ID" value="NP_6108A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694614"/>
    <dbReference type="KEGG" id="nph:NP_6108A"/>
    <dbReference type="HOGENOM" id="CLU_147145_0_0_2"/>
    <dbReference type="OrthoDB" id="202478at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="1">
        <location>
            <begin position="6"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="148" mass="16100" checksum="7E627DBCE139230F" modified="2005-11-08" version="1">MTDLLQRTLVPVKNSDDAIATCTALADYLDGDTKLVAILHVAEQTEGYMDHASPEALRERANELFALCGKQLDESLPVRTELRFGTDVVEEIAEAAVELDVDAIVFRPCSERRLTDLLSGDAERRLLRNAPCPVVTLSDDGGVSEVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM47</accession>
    <name>Q3IM47_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_6072A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324420.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM47"/>
    <dbReference type="EnsemblBacteria" id="CAI50816">
        <property type="protein sequence ID" value="CAI50816"/>
        <property type="gene ID" value="NP_6072A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694701"/>
    <dbReference type="KEGG" id="nph:NP_6072A"/>
    <dbReference type="HOGENOM" id="CLU_1840641_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="139" mass="14901" checksum="D0E6B3170C36E823" modified="2005-11-08" version="1">MNRRAALTVVTTATASALAGCGGVDIGTSGAGDEEDDHKETDANNNGASQESDAPTHDPPCDSPAYIFDQDNQRISFDFAMWMIWGDINTTSSSMNRERGNVSDIEWNVGDKIARDNSVYEIQVADGALVMEPTNGGQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM55</accession>
    <name>Q3IM55_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_6054A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50807.3"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939906.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM55"/>
    <dbReference type="EnsemblBacteria" id="CAI50807">
        <property type="protein sequence ID" value="CAI50807"/>
        <property type="gene ID" value="NP_6054A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694694"/>
    <dbReference type="KEGG" id="nph:NP_6054A"/>
    <dbReference type="HOGENOM" id="CLU_2243940_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50807.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="104" mass="10710" checksum="B50BEFC81F009182" modified="2012-10-03" version="2">MIRFNVADGPLAVLEDSPAAAPFEQLAEAVADRVGVRNRAEVADIDPEDAPGEPTGTESYLEAATTESQEADVASKSSRSDSDSSPEDDAGTDGLSGGLDISEK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-01-10" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q3INY2</accession>
    <name>HIS6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Imidazole glycerol phosphate synthase subunit HisF</fullName>
            <ecNumber evidence="1">4.3.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">IGP synthase cyclase subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">IGP synthase subunit HisF</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">ImGP synthase subunit HisF</fullName>
            <shortName evidence="1">IGPS subunit HisF</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisF</name>
        <name type="ordered locus">NP_4158A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine = D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="4.3.2.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of HisH and HisF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HisA/HisF family.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.10" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323786.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3INY2"/>
    <dbReference type="SMR" id="Q3INY2"/>
    <dbReference type="STRING" id="348780.NP_4158A"/>
    <dbReference type="EnsemblBacteria" id="CAI50170">
        <property type="protein sequence ID" value="CAI50170"/>
        <property type="gene ID" value="NP_4158A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702819"/>
    <dbReference type="KEGG" id="nph:NP_4158A"/>
    <dbReference type="eggNOG" id="arCOG00617">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048577_4_0_2"/>
    <dbReference type="OrthoDB" id="6261at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000107">
        <property type="term" value="F:imidazoleglycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04731">
        <property type="entry name" value="HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01013">
        <property type="entry name" value="HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004651">
        <property type="entry name" value="HisF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050064">
        <property type="entry name" value="IGPS_HisA/HisF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00735">
        <property type="entry name" value="hisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21235:SF2">
        <property type="entry name" value="IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE HISHF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21235">
        <property type="entry name" value="IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE SUBUNIT HISF/H IGP SYNTHASE SUBUNIT HISF/H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000142285" description="Imidazole glycerol phosphate synthase subunit HisF">
        <location>
            <begin position="1"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01013"/>
        </source>
    </evidence>
    <sequence length="271" mass="29048" checksum="A4506269974821D7" modified="2005-11-08" version="1">MALTKRIIPCIDVDLNEDGEAAVYTGVNFEDLEYTGDPVELAKKYNEAGADEFVFLDITASAEGRETMLDTVSAVADECFIPLTVGGGIRTRDDIKETLRAGADKVSINTGALQRPELIGEGAEAFGSQCIVISVDARRRFDEAGEHYVEVDGESCWFECTIKGGREGTGTDVVEWAGEAADRGAGELFVNSIDADGTKDGYDIPLTKAVCDNVPTPVIASSGCGSPEHMAEVFEDADADAALAASIFHFEEYSIEETKRSLDEQGIPVRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IPF9</accession>
    <name>Q3IPF9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Homolog to ornithine cyclodeaminase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3802A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ornithine cyclodeaminase/mu-crystallin family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323609.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IPF9"/>
    <dbReference type="STRING" id="348780.NP_3802A"/>
    <dbReference type="EnsemblBacteria" id="CAI49992">
        <property type="protein sequence ID" value="CAI49992"/>
        <property type="gene ID" value="NP_3802A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701869"/>
    <dbReference type="KEGG" id="nph:NP_3802A"/>
    <dbReference type="eggNOG" id="arCOG01035">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042088_2_0_2"/>
    <dbReference type="OrthoDB" id="182805at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000311">
        <property type="entry name" value="Ornithine cyclodeaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1780.10">
        <property type="entry name" value="ornithine cyclodeaminase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003462">
        <property type="entry name" value="ODC_Mu_crystall"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023401">
        <property type="entry name" value="ODC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13812">
        <property type="entry name" value="KETIMINE REDUCTASE MU-CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13812:SF19">
        <property type="entry name" value="KETIMINE REDUCTASE MU-CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02423">
        <property type="entry name" value="OCD_Mu_crystall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001439">
        <property type="entry name" value="CryM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="332" mass="34488" checksum="723844C784711F58" modified="2005-11-08" version="1">MTDVLFLRSDELAGLATPAEYVDAVRDGYESYGTDGTAAPRTKLTNEEPPGMCTGYLAVLPSVGAMGGYTYAAGFGDGDVHFALPLFDAESGRMIALLDGASMNPHKTGATGAVAVDALAREDATTCAVIGSGAQARGQLRATATVREFETVQVFSPTLESRQSFADEFDDRLAAAVSAVPSAADALDGADVVITATRASTPVFEDADLPDGAHVTAMGQYDRGKYELPPETVARSTYVPDLHDRAFQDAGAFLAARDAGLVDDDHIRADLGDIVAGEAAGRTSDEAITVFDSGGTGVETVAAAWMLYEKAREAGLGERIEFAPGSEALTGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-13" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IQU8</accession>
    <name>SYY_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tyrosine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Tyrosyl-tRNA synthetase</fullName>
            <shortName evidence="1">TyrRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tyrS</name>
        <name type="ordered locus">NP_2810A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Tyr) + L-tyrosine + ATP = L-tyrosyl-tRNA(Tyr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10220"/>
            <dbReference type="Rhea" id="RHEA-COMP:9706"/>
            <dbReference type="Rhea" id="RHEA-COMP:9707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78536"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.1" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323121.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IQU8"/>
    <dbReference type="SMR" id="Q3IQU8"/>
    <dbReference type="STRING" id="348780.NP_2810A"/>
    <dbReference type="EnsemblBacteria" id="CAI49496">
        <property type="protein sequence ID" value="CAI49496"/>
        <property type="gene ID" value="NP_2810A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703432"/>
    <dbReference type="KEGG" id="nph:NP_2810A"/>
    <dbReference type="eggNOG" id="arCOG01886">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035267_0_1_2"/>
    <dbReference type="OrthoDB" id="8389at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004831">
        <property type="term" value="F:tyrosine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006437">
        <property type="term" value="P:tyrosyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02008">
        <property type="entry name" value="Tyr_tRNA_synth_type3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002307">
        <property type="entry name" value="Tyr-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023684">
        <property type="entry name" value="Tyr-tRNA-ligase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023617">
        <property type="entry name" value="Tyr-tRNA-ligase_arc/euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050489">
        <property type="entry name" value="Tyr-tRNA_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006330">
        <property type="entry name" value="PRK08560.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00234">
        <property type="entry name" value="tyrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46264:SF4">
        <property type="entry name" value="TYROSINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46264">
        <property type="entry name" value="TYROSINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006588">
        <property type="entry name" value="TyrRS_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01040">
        <property type="entry name" value="TRNASYNTHTYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000240261" description="Tyrosine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="41"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02008"/>
        </source>
    </evidence>
    <sequence length="345" mass="37891" checksum="AD9478B4F28339D8" modified="2005-11-08" version="1">MDTVERTDLVARFTEEVIERDEIETLFEEQDEPTAYIGYAPTGEMHIGHFTTMRKLADFIDAGLDVTVLVADLHAHLDDAKSPFELLEARSEYYERAIEGMIAAAGADPDGISFIRGTDFQLDEPYTLDLYRLLADTTLSRAQRAGSEVVRQSENPSLGSLVYTLMQALDVAALDADIAYGGIDQRGIYMLAREQLPDHGYDKPACVFAPLLSGLSGGKMSASEAGSKINLTDDGEAIDEKIGGAYCPAGETEENGVLEYLEYLVFPVFDQRDTSFVVERPEKYGGDLEYGTYDELEADFVSGELHPADLKPAAAAAIDEVVAPVRELLLEDPELLASAYPERYE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-16" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IRX5</accession>
    <name>GLYA_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine hydroxymethyltransferase</fullName>
            <shortName evidence="1">SHMT</shortName>
            <shortName evidence="1">Serine methylase</shortName>
            <ecNumber evidence="1">2.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">glyA</name>
        <name type="ordered locus">NP_2050A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:15481"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SHMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.1" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322745.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IRX5"/>
    <dbReference type="SMR" id="Q3IRX5"/>
    <dbReference type="STRING" id="348780.NP_2050A"/>
    <dbReference type="EnsemblBacteria" id="CAI49116">
        <property type="protein sequence ID" value="CAI49116"/>
        <property type="gene ID" value="NP_2050A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702765"/>
    <dbReference type="KEGG" id="nph:NP_2050A"/>
    <dbReference type="eggNOG" id="arCOG00070">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022477_2_1_2"/>
    <dbReference type="OrthoDB" id="5821at2157"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="UniPathway" id="UPA00288">
        <property type="reaction ID" value="UER01023"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000001">
        <property type="entry name" value="Serine hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049943">
        <property type="entry name" value="Ser_HO-MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000586">
        <property type="entry name" value="PRK00011.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680:SF35">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000235056" description="Serine hydroxymethyltransferase">
        <location>
            <begin position="1"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>(6S)-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="123"/>
            <end position="125"/>
        </location>
        <ligand>
            <name>(6S)-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="353"/>
            <end position="355"/>
        </location>
        <ligand>
            <name>(6S)-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
        </ligand>
    </feature>
    <feature type="site" description="Plays an important role in substrate specificity" evidence="1">
        <location>
            <position position="227"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00051"/>
        </source>
    </evidence>
    <sequence length="424" mass="45731" checksum="727609956D2CF67F" modified="2005-11-08" version="1">MNYEQVREADPAIAAALADERDRQEDTLAMIASENHVSEAVLQAQSSELTNKYAEGYPGERYYAGCGPADDVEELAIERAEELWGAEHINVQPHSGTQANMAVYLAMLEPGDRILSLELEHGGHLSHGHPANFTGQTYEVEQYEVDPETGYIDYDELHEQAEAFEPDIIVSGYSAYPREVEFERIQEAADAVDAYHLADIAHITGLVAAGVHQSPVGVADFVTGSTHKTIRAGRGGIVMCDEEYADDIDAAVFPGAQGGPLMHNVAGKAVGFKEALQPEFEQYAQQVIDNAEALGERLQEHGFSLVSGGTDNHLVLVDLRESHPDTSGTVAEEALEAAGIVLNKNTVPGETRSAFNPSGIRAGTPALTTRGFDEQACERVADIIANVIDNPDDEGTIDEAAAEVDALCEEYPLYQGESGITDFE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-02" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IRZ6</accession>
    <name>URE2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Urease subunit beta</fullName>
            <ecNumber evidence="1">3.5.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Urea amidohydrolase subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ureB</name>
        <name type="ordered locus">NP_2008A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>urea + 2 H2O + H(+) = hydrogencarbonate + 2 NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:20557"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16199"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (urease route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the urease beta subunit family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.5" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322724.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IRZ6"/>
    <dbReference type="SMR" id="Q3IRZ6"/>
    <dbReference type="STRING" id="348780.NP_2008A"/>
    <dbReference type="EnsemblBacteria" id="CAI49095">
        <property type="protein sequence ID" value="CAI49095"/>
        <property type="gene ID" value="NP_2008A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703449"/>
    <dbReference type="KEGG" id="nph:NP_2008A"/>
    <dbReference type="eggNOG" id="arCOG04527">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129707_2_1_2"/>
    <dbReference type="OrthoDB" id="2598at2157"/>
    <dbReference type="UniPathway" id="UPA00258">
        <property type="reaction ID" value="UER00370"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035550">
        <property type="term" value="C:urease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009039">
        <property type="term" value="F:urease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043419">
        <property type="term" value="P:urea catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00407">
        <property type="entry name" value="Urease_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.150.10:FF:000001">
        <property type="entry name" value="Urease subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.150.10">
        <property type="entry name" value="Urease, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01954">
        <property type="entry name" value="Urease_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002019">
        <property type="entry name" value="Urease_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036461">
        <property type="entry name" value="Urease_betasu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050069">
        <property type="entry name" value="Urease_subunit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009682">
        <property type="entry name" value="PRK13203.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00192">
        <property type="entry name" value="urease_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33569">
        <property type="entry name" value="UREASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33569:SF1">
        <property type="entry name" value="UREASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00699">
        <property type="entry name" value="Urease_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51278">
        <property type="entry name" value="Urease, beta-subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000234284" description="Urease subunit beta">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01954"/>
        </source>
    </evidence>
    <sequence length="132" mass="14144" checksum="FED3BE2C187922EB" modified="2005-11-08" version="1">MSDPSEGSLTPGELLVDDAPVELNAGRETATVTVENTGDRPVQVGSHYHFFETNPALAFDRETAYGMRLNIPAGTAVRFEPGAERDVELVAIGGDRIVHGMDGLVNGDLDDEATRERAMDRAAAAGYRGVER</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-16" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IT59</accession>
    <name>SYDND_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aspartate--tRNA(Asp/Asn) ligase</fullName>
            <ecNumber evidence="1">6.1.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Aspartyl-tRNA synthetase</fullName>
            <shortName evidence="1">AspRS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Non-discriminating aspartyl-tRNA synthetase</fullName>
            <shortName evidence="1">ND-AspRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aspS</name>
        <name type="ordered locus">NP_1168A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Asx) + L-aspartate + ATP = L-aspartyl-tRNA(Asx) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:18349"/>
            <dbReference type="Rhea" id="RHEA-COMP:9710"/>
            <dbReference type="Rhea" id="RHEA-COMP:9711"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 3 Mg(2+) cations per subunit. The strongest magnesium site (Mg1) is bound to the beta- and gamma-phosphates of ATP and four water molecules complete its coordination sphere.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.23" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322311.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IT59"/>
    <dbReference type="SMR" id="Q3IT59"/>
    <dbReference type="STRING" id="348780.NP_1168A"/>
    <dbReference type="EnsemblBacteria" id="CAI48675">
        <property type="protein sequence ID" value="CAI48675"/>
        <property type="gene ID" value="NP_1168A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702537"/>
    <dbReference type="KEGG" id="nph:NP_1168A"/>
    <dbReference type="eggNOG" id="arCOG00406">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004553_2_1_2"/>
    <dbReference type="OrthoDB" id="5908at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004815">
        <property type="term" value="F:aspartate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050560">
        <property type="term" value="F:aspartate-tRNA(Asn) ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006422">
        <property type="term" value="P:aspartyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00776">
        <property type="entry name" value="AsxRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04316">
        <property type="entry name" value="ND_PkAspRS_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000038">
        <property type="entry name" value="Aspartate--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02075">
        <property type="entry name" value="Asp_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004523">
        <property type="entry name" value="Asp-tRNA_synthase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00458">
        <property type="entry name" value="aspS_nondisc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003483">
        <property type="entry name" value="PRK05159.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450:SF1">
        <property type="entry name" value="ASPARTATE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450">
        <property type="entry name" value="ASPARTYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000235586" description="Aspartate--tRNA(Asp/Asn) ligase">
        <location>
            <begin position="1"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Aspartate" evidence="1">
        <location>
            <begin position="189"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="211"/>
            <end position="213"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="219"/>
            <end position="221"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="359"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="359"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="404"/>
            <end position="407"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for tRNA non-discrimination" evidence="1">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02075"/>
        </source>
    </evidence>
    <sequence length="433" mass="48479" checksum="05DA4D3F697D6350" modified="2005-11-08" version="1">MENRTYTADAEPGETVTVAGWVHEVRDLGGIAFLILRDTTGQIQVKFEKDEMDDELVETGLNVARESVITVSGDVEEEPRAPTGVEVTPETVEVLSEADTELPLDPTGKVDAELSTRLDNRTLDLRSEEGQAIFEIRAEVLRAVREAFRDANATEINTSKIVATGTEGGTELFPITYFGEEAFMNQSPQLFKQLVAGSNIERVFEIGPIFRAEEHNTPRHLNEATSIDFEGAFCDHTDAMDVCEQVVTAAYEAVAENCTDQLEALGITEEFEVPETPFPRLSYEEAIERINATGELDEQLVWGDDLPTEGEKALGDDVGGHYFITDWPAEIKPFYIMDHEDGELSTGFDMMHPRMELVSGGQREHRREELIAGFEQQGLEPEAFEYYTKMFKYGMPPHAGWGLGGERLLMTMLDLDNIREAVLFPRDRQRLSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ITB7</accession>
    <name>Q3ITB7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoesterase</fullName>
            <ecNumber evidence="1">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_1052A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallophosphoesterase superfamily. YfcE family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322252.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ITB7"/>
    <dbReference type="STRING" id="348780.NP_1052A"/>
    <dbReference type="EnsemblBacteria" id="CAI48617">
        <property type="protein sequence ID" value="CAI48617"/>
        <property type="gene ID" value="NP_1052A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701039"/>
    <dbReference type="KEGG" id="nph:NP_1052A"/>
    <dbReference type="eggNOG" id="arCOG01141">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063749_3_2_2"/>
    <dbReference type="OrthoDB" id="9959at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000979">
        <property type="entry name" value="Phosphodiesterase_MJ0936/Vps29"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00040">
        <property type="entry name" value="yfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11124">
        <property type="entry name" value="VACUOLAR SORTING PROTEIN VPS29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU362039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="163" mass="17015" checksum="D3BDB8AF57C54563" modified="2005-11-08" version="1">MEVAILADTHVMSRAAAIPDWVTETVQSADHVIHAGDFDSRPAYEELDSLAASLTAVAGNMDHGLDLPTVATVDLAGVRFVVTHGDGPDEGYKERLAAITDTHAAGTTVGVGGHTHRVLDTEVDGYRLCNPGSATAAWPAQEETMLVATVAGGDVELALRRGD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ITN9</accession>
    <name>AROE_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Shikimate dehydrogenase (NADP(+))</fullName>
            <shortName evidence="1">SDH</shortName>
            <ecNumber evidence="1">1.1.1.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aroE</name>
        <name type="ordered locus">NP_0806A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>shikimate + NADP(+) = 3-dehydroshikimate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17737"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the shikimate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.25" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322130.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ITN9"/>
    <dbReference type="SMR" id="Q3ITN9"/>
    <dbReference type="STRING" id="348780.NP_0806A"/>
    <dbReference type="EnsemblBacteria" id="CAI48494">
        <property type="protein sequence ID" value="CAI48494"/>
        <property type="gene ID" value="NP_0806A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702529"/>
    <dbReference type="KEGG" id="nph:NP_0806A"/>
    <dbReference type="eggNOG" id="arCOG01033">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044063_1_1_2"/>
    <dbReference type="OrthoDB" id="8744at2157"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00087"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004764">
        <property type="term" value="F:shikimate 3-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019632">
        <property type="term" value="P:shikimate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01065">
        <property type="entry name" value="NAD_bind_Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00222">
        <property type="entry name" value="Shikimate_DH_AroE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041121">
        <property type="entry name" value="SDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011342">
        <property type="entry name" value="Shikimate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013708">
        <property type="entry name" value="Shikimate_DH-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022893">
        <property type="entry name" value="Shikimate_DH_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006151">
        <property type="entry name" value="Shikm_DH/Glu-tRNA_Rdtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00507">
        <property type="entry name" value="aroE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001319">
        <property type="entry name" value="PRK00258.3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21089:SF1">
        <property type="entry name" value="BIFUNCTIONAL 3-DEHYDROQUINATE DEHYDRATASE_SHIKIMATE DEHYDROGENASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21089">
        <property type="entry name" value="SHIKIMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18317">
        <property type="entry name" value="SDH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01488">
        <property type="entry name" value="Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08501">
        <property type="entry name" value="Shikimate_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000021310" description="Shikimate dehydrogenase (NADP(+))">
        <location>
            <begin position="1"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="14"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="122"/>
            <end position="126"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="145"/>
            <end position="150"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00222"/>
        </source>
    </evidence>
    <sequence length="264" mass="27690" checksum="9984AC20F0AEBF25" modified="2005-11-08" version="1">MDVYGLIGNPVEHSVSPPMHEAAYEALGLDARYVTFEPASEQLDTALEGASALGVRGLNVTIPFKEDVLDVVEPDPLAERIGAVNTIDFEGEPTGHNTDAAGVIRAFQHHDVERSGTAVVVGAGGAGRAAAFALADEGMEVHIANRTVSRATALATEVPDATGHGLDELAELVPDADVLVNATSVGMEADETPVPKRHLHEGLAVLDAVYAPLETRLLREADAAGATTIDGAWMLLFQGVEAFEIWTGKAAPVEPMNEALRSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ET93</accession>
    <name>A0A1U7ET93_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Receiver box response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_0028A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321744.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ET93"/>
    <dbReference type="EnsemblBacteria" id="CAI48105">
        <property type="protein sequence ID" value="CAI48105"/>
        <property type="gene ID" value="NP_0028A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700741"/>
    <dbReference type="KEGG" id="nph:NP_0028A"/>
    <dbReference type="eggNOG" id="arCOG02597">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114810_0_0_2"/>
    <dbReference type="OrthoDB" id="86314at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050595">
        <property type="entry name" value="Bact_response_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013971">
        <property type="entry name" value="HalX_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44591:SF3">
        <property type="entry name" value="RESPONSE REGULATORY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44591">
        <property type="entry name" value="STRESS RESPONSE REGULATOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08663">
        <property type="entry name" value="HalX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="7"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="146"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI48105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="188" mass="21147" checksum="5F163ECC8A42F430" modified="2017-05-10" version="1">MTDEQPTVLVVDDEPELTDLYATWLESGYDVRTAYGGPEAVERLNEDIDVALVDRLMPQTSGDEVLDHVRAADYDCRVAMVTSVEPDFDIIDLSFDEYAVKPVRKDELESVVEALVARSEYDSQVQRLFALASKRAALRAEKADEKLEQSDAYQQLTADIDQLQSELEATLSQLDARDLEAEMQRLIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETE8</accession>
    <name>A0A1U7ETE8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC-type transport system periplasmic substrate-binding protein (Probable substrate branched-chain amino acids)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">livJ5</name>
        <name evidence="3" type="synonym">abc09s</name>
        <name evidence="3" type="ordered locus">NP_0174A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321817.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETE8"/>
    <dbReference type="STRING" id="348780.NP_0174A"/>
    <dbReference type="EnsemblBacteria" id="CAI48178">
        <property type="protein sequence ID" value="CAI48178"/>
        <property type="gene ID" value="NP_0174A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702396"/>
    <dbReference type="KEGG" id="nph:NP_0174A"/>
    <dbReference type="eggNOG" id="arCOG01024">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027128_1_4_2"/>
    <dbReference type="OrthoDB" id="264684at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051010">
        <property type="entry name" value="BCAA_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028081">
        <property type="entry name" value="Leu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30483:SF37">
        <property type="entry name" value="ABC TRANSPORTER SUBSTRATE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30483">
        <property type="entry name" value="LEUCINE-SPECIFIC-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13458">
        <property type="entry name" value="Peripla_BP_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Leucine-binding protein" evidence="2">
        <location>
            <begin position="51"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="438" mass="47143" checksum="234F26A4331A7B85" modified="2017-05-10" version="1">MTDSFNLDRRNVLKAAGGTAVAAALAGCSVLLDGEENGNGGAALEDVPDEPIEAGLQTFREGAPSVLGLQAEYGAQTAIRRINDAGGIAGRQMELDVIEEEGATIENYRRFVDEGKDVTFGPISSGGHEELVPVVEEEGVVNVGTDGTVTTLYETEYPDVEYSFRFQNHDVMEALSAARTAVEQLGAENIDTFANINPDYTFGYDEREVFNAGIEQLTGAEEVYDGYPELGADDMSTHVTAVADAEPDVLFTSCWGGDATLLLDQGQAGDLFDAVDITVGPVLYSAANDFSEELVEGNIWSGSRNFYWGSPPQNQWDPGVELFEEAQDEHDTIPTAHFMSGYGAVTAWATAVEKAVDIVGGWPSQDQIAATLENHGFYTPAGYHTIGPDHQGYSTAHFGEMTWDDGMDMPVLENVTTIPASHVSPPQGEISIDWIESW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETN3</accession>
    <name>A0A1U7ETN3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0370A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321914.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETN3"/>
    <dbReference type="STRING" id="348780.NP_0370A"/>
    <dbReference type="EnsemblBacteria" id="CAI48276">
        <property type="protein sequence ID" value="CAI48276"/>
        <property type="gene ID" value="NP_0370A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700832"/>
    <dbReference type="KEGG" id="nph:NP_0370A"/>
    <dbReference type="eggNOG" id="arCOG04588">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104913_0_0_2"/>
    <dbReference type="OrthoDB" id="213344at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043815">
        <property type="entry name" value="DUF5797"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19110">
        <property type="entry name" value="DUF5797"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="161" mass="17783" checksum="AF897472BF9AD67A" modified="2017-05-10" version="1">MTLSEEAESRLADIVSLQPTKNSELQERWGLDGGSEVHQYLESELKEYYYRDENSLIRATPEAAELVGIDTDEDIVRVPPLQSDIIDVLAGPDGEPQSVVSVLHDLEDAGIETDVDAVRSGLRSLEDKGIVAVIKKTVPTFRLAVERDDIEVETLEEPAHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETR4</accession>
    <name>A0A1U7ETR4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ParA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">parA3</name>
        <name evidence="3" type="ordered locus">NP_0438A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321946.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETR4"/>
    <dbReference type="STRING" id="348780.NP_0438A"/>
    <dbReference type="EnsemblBacteria" id="CAI48310">
        <property type="protein sequence ID" value="CAI48310"/>
        <property type="gene ID" value="NP_0438A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700856"/>
    <dbReference type="KEGG" id="nph:NP_0438A"/>
    <dbReference type="eggNOG" id="arCOG00589">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_658276_0_0_2"/>
    <dbReference type="OrthoDB" id="31168at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051782">
        <property type="term" value="P:negative regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050625">
        <property type="entry name" value="ParA/MinD_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384:SF10">
        <property type="entry name" value="ATPASE INVOLVED IN CHROMOSOME PARTITIONING, PARA_MIND FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384">
        <property type="entry name" value="SEPTUM SITE-DETERMINING PROTEIN MIND HOMOLOG, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobQ/CobB/MinD/ParA nucleotide binding" evidence="2">
        <location>
            <begin position="6"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="258"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="298"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="387"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="417" mass="43326" checksum="8E3BAEB66C4F9FFC" modified="2017-05-10" version="1">MSGDVLVVTSGKGGVGKTTTVVNLAIALRQHGHSVAVLDADLGMPDVGEFLSIDAKPTLHDVLAGRADITEATVEIGDGLAFVFGDTSLEGFAQADPAKLEAVISDLTDEYQCVLVDTGGGLTYETVFPMDLGDAVLLVTSPVPAAIADTKKSKQVAERLGVPVCGVVVTHADGDAHPESVASELDVDFLGSVPDDEAIVESAAKRQPVVAYAPESAATVAYYRLAERIADEETAALEPLDTAAKAPEEVFESATDITDVDDSDDATESEADGESDAEPDTEAERDESGDEAEPRGAEDDDASEADSEAAVGESENEVEDTGGDGRTEADDGKRHGVDDTSDADDEKPAADEPTTEAKTPAEMAGGIDEPEPGLHTGGTDDADASEEGARGGRDDTETEDGSKDDEGFFDRLFSRFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETS9</accession>
    <name>A0A1U7ETS9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">MaoC domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">maoC5</name>
        <name evidence="3" type="ordered locus">NP_0488A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321971.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETS9"/>
    <dbReference type="STRING" id="348780.NP_0488A"/>
    <dbReference type="EnsemblBacteria" id="CAI48335">
        <property type="protein sequence ID" value="CAI48335"/>
        <property type="gene ID" value="NP_0488A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702690"/>
    <dbReference type="KEGG" id="nph:NP_0488A"/>
    <dbReference type="eggNOG" id="arCOG00778">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094876_3_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019171">
        <property type="term" value="F:(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03449">
        <property type="entry name" value="R_hydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002539">
        <property type="entry name" value="MaoC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050965">
        <property type="entry name" value="UPF0336/Enoyl-CoA_hydratase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43437:SF3">
        <property type="entry name" value="HYDROXYACYL-THIOESTER DEHYDRATASE TYPE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43437">
        <property type="entry name" value="HYDROXYACYL-THIOESTER DEHYDRATASE TYPE 2, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01575">
        <property type="entry name" value="MaoC_dehydratas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MaoC-like" evidence="2">
        <location>
            <begin position="34"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="157" mass="16609" checksum="CA4F0A80239D0E5B" modified="2017-05-10" version="1">MTEEETGPDVPIAAGEDRPDWETESDIDGEPSVGDTLRFSKPVSEADVERFALASGDTNPLHLDEASAAGTRFGGRIVHGGLIAGIISAALARLPGTVVYLEQDLTFRSPVRIGETVTAELEVVDDLSEAKYRLRTVARTDGDAAVEGTATVLIEDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETW6</accession>
    <name>A0A1U7ETW6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Dihydrolipoamide S-acyltransferase</fullName>
            <ecNumber evidence="9">2.3.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">dsa</name>
        <name evidence="9" type="ordered locus">NP_0556A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="9"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322005.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETW6"/>
    <dbReference type="STRING" id="348780.NP_0556A"/>
    <dbReference type="EnsemblBacteria" id="CAI48369">
        <property type="protein sequence ID" value="CAI48369"/>
        <property type="gene ID" value="NP_0556A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702671"/>
    <dbReference type="KEGG" id="nph:NP_0556A"/>
    <dbReference type="eggNOG" id="arCOG01706">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016733_10_1_2"/>
    <dbReference type="OrthoDB" id="56234at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031405">
        <property type="term" value="F:lipoic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06849">
        <property type="entry name" value="lipoyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000036">
        <property type="entry name" value="Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000007">
        <property type="entry name" value="Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.10">
        <property type="entry name" value="E3-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050743">
        <property type="entry name" value="2-oxoacid_DH_E2_comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036625">
        <property type="entry name" value="E3-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004167">
        <property type="entry name" value="PSBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43178">
        <property type="entry name" value="DIHYDROLIPOAMIDE ACETYLTRANSFERASE COMPONENT OF PYRUVATE DEHYDROGENASE COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43178:SF5">
        <property type="entry name" value="LIPOAMIDE ACYLTRANSFERASE COMPONENT OF BRANCHED-CHAIN ALPHA-KETO ACID DEHYDROGENASE COMPLEX, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02817">
        <property type="entry name" value="E3_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47005">
        <property type="entry name" value="Peripheral subunit-binding domain of 2-oxo acid dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51826">
        <property type="entry name" value="PSBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="7">
        <location>
            <begin position="2"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Peripheral subunit-binding (PSBD)" evidence="8">
        <location>
            <begin position="121"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="79"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="138"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="79"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="109"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="157"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="176"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="186"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="207"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI48369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="516" mass="54802" checksum="B5F6FA968898804D" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETZ8</accession>
    <name>A0A1U7ETZ8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UspA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0656A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322055.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETZ8"/>
    <dbReference type="STRING" id="348780.NP_0656A"/>
    <dbReference type="EnsemblBacteria" id="CAI48419">
        <property type="protein sequence ID" value="CAI48419"/>
        <property type="gene ID" value="NP_0656A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700918"/>
    <dbReference type="KEGG" id="nph:NP_0656A"/>
    <dbReference type="eggNOG" id="arCOG02053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_11_1_2"/>
    <dbReference type="OrthoDB" id="105697at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF6">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN UP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006276">
        <property type="entry name" value="UspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="143" mass="14633" checksum="86221D880167FDDA" modified="2017-05-10" version="1">MYSTILLATDGSEDSRAALDHAVEVAAATGASLHVVSVVETRTAYDNAIIDPEEVKENLRADAVEAVEAAAAVAAAEDVSCETTVEEGVPPEQVLEVAERVGADLVVVGATGRSGFKRLVLGSTAEQLLESSGTAPVVVVADE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU04</accession>
    <name>A0A1U7EU04_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">NP_1176A family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0674A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322064.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU04"/>
    <dbReference type="STRING" id="348780.NP_0674A"/>
    <dbReference type="EnsemblBacteria" id="CAI48428">
        <property type="protein sequence ID" value="CAI48428"/>
        <property type="gene ID" value="NP_0674A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700925"/>
    <dbReference type="KEGG" id="nph:NP_0674A"/>
    <dbReference type="eggNOG" id="arCOG00394">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_176006_1_0_2"/>
    <dbReference type="OrthoDB" id="350804at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="93" mass="10179" checksum="43E1283F47140A8D" modified="2017-05-10" version="1">MADSDGEALADLPPSAKLVYKVLEYNGAMTQKDIVEESMLSARTVRYALERLEDIDIVSEDVYFADARQNLYELTDEAEAVTDGAADPAACDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU15</accession>
    <name>A0A1U7EU15_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0662A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48422.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939433.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU15"/>
    <dbReference type="STRING" id="348780.NP_0662A"/>
    <dbReference type="EnsemblBacteria" id="CAI48422">
        <property type="protein sequence ID" value="CAI48422"/>
        <property type="gene ID" value="NP_0662A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700920"/>
    <dbReference type="KEGG" id="nph:NP_0662A"/>
    <dbReference type="eggNOG" id="arCOG09246">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1056083_0_0_2"/>
    <dbReference type="OrthoDB" id="238312at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48422.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="255" mass="27257" checksum="7FC00BD7038AA779" modified="2017-05-10" version="1">MNANSPLVGGGDPASTVFAARVLAHASIAVAAAILLVFLGVYVRRVLAEGFEYEWGFLAVGIAAAIVYGIAGLAADLTGSAWLAVFAEGAVLFFILFIALGIRAMYHADHPTGSSRLLPKWVDALVIVGFIAAWWVGYLAGGEWTRPVVAVGWIGASIWAVFYSVRTTQAHEGTTFSALTRHLLPAILCIVAVTMTDLATTVAAVDQSAVEAVWLVGTVLVAAFLFNTAVAIRQQEGEVERMYDRTTWRQQDIDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU25</accession>
    <name>A0A1U7EU25_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UspA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0690A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322072.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU25"/>
    <dbReference type="STRING" id="348780.NP_0690A"/>
    <dbReference type="EnsemblBacteria" id="CAI48436">
        <property type="protein sequence ID" value="CAI48436"/>
        <property type="gene ID" value="NP_0690A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700931"/>
    <dbReference type="KEGG" id="nph:NP_0690A"/>
    <dbReference type="eggNOG" id="arCOG02053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_11_1_2"/>
    <dbReference type="OrthoDB" id="105697at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF6">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN UP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="151" mass="16603" checksum="4ACFE983B1705C78" modified="2017-05-10" version="1">MYDRILLPTDMSAGVDRAIEHAIDAAQRYDAELHVLYVVDTDAYSSYPGDEYVHEFEGLEQALEQAGGDAVEDIAEAAADADVAAETTIQHGVPHEEILDYIDEKAIDLTVVGSKNRSEEYRRLLGSVAERVVHMSEKPVTVVKTPVEADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU49</accession>
    <name>A0A1U7EU49_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0782A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322118.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU49"/>
    <dbReference type="STRING" id="348780.NP_0782A"/>
    <dbReference type="EnsemblBacteria" id="CAI48482">
        <property type="protein sequence ID" value="CAI48482"/>
        <property type="gene ID" value="NP_0782A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700962"/>
    <dbReference type="KEGG" id="nph:NP_0782A"/>
    <dbReference type="eggNOG" id="arCOG02079">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028008_1_0_2"/>
    <dbReference type="OrthoDB" id="56770at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35902:SF3">
        <property type="entry name" value="NPCBM-ASSOCIATED, NEW3 DOMAIN OF ALPHA-GALACTOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35902">
        <property type="entry name" value="S-LAYER DOMAIN-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="481"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="514" mass="55335" checksum="6DD650C27944971E" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU55</accession>
    <name>A0A1U7EU55_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC-type transport system permease protein (Probable substrate dipeptide/oligopeptide)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">dppC1</name>
        <name evidence="9" type="synonym">abc01p2</name>
        <name evidence="9" type="ordered locus">NP_0762A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
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    </evidence>
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    </evidence>
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        <source>
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    <sequence length="357" mass="38311" checksum="E0E9D674DADE891D" modified="2017-05-10" version="1">MISDRAKQSLSLELKRSRLAQIGVAIMAVMFVVTMFAPLFAPHDPEQQRVGTGDTEDMTDLPPVGFSTTANETRLVDGEFQTQQVEINANSSHVLGTNTRGQDVLSRVIYGGRVSLLVGLLGAAIAALVGVPYGLAAGYFSGRVDDALMRGADIMLAFPSLVLAIALVGVFRETDFHTMELTDPFVVAAQSETIPNWLIPRAGHVASMPESVTFPVTVTLVVALVNWVWFARVARGEAMSIRSEEYVTAAKSLGASNWTIIRKHVLPNSITPIIVLATIQVAFIILLESALSFLGFSGTTLTWGQDIARGQSDQRAQWWIATVPGLAIVLAVIGVNLIGDWLRDALDPDIEGEGGGV</sequence>
</entry>
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        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
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            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
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    <dbReference type="KEGG" id="nph:NP_0830A"/>
    <dbReference type="eggNOG" id="arCOG01541">
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="ND/Mrp_TM"/>
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        <property type="entry name" value="PRK07234.1-1"/>
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    </dbReference>
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    </dbReference>
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        </location>
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            <begin position="492"/>
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        </location>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="568" mass="60332" checksum="C7C55A5259F70AD8" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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        </submittedName>
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    <organism evidence="1 2">
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        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAI48529"/>
        <property type="gene ID" value="NP_0876A"/>
    </dbReference>
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    <dbReference type="KEGG" id="nph:NP_0876A"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="150" mass="16567" checksum="E2CD394C41843528" modified="2017-05-10" version="1">MATWNGAWSKAEATAYLEGQTVPIRLACRTPSGGLWMLSLWYQYQDGTLACATSADADVVSFLEADDGVAFEVSDNQPPYRGVRGAGTVTISSDEEKTLLRSLLDRYVGGTDNDLGNQLLSADRKEVRLEIEPDRLYSWDFSDRMSDIDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUF8</accession>
    <name>A0A1U7EUF8_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Large ribosomal subunit protein eL39</fullName>
        </recommendedName>
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        <name evidence="5 7" type="primary">rpl39e</name>
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        <lineage>
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        </lineage>
    </organism>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eL39 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322232.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUF8"/>
    <dbReference type="STRING" id="348780.NP_1012A"/>
    <dbReference type="EnsemblBacteria" id="CAI48597">
        <property type="protein sequence ID" value="CAI48597"/>
        <property type="gene ID" value="NP_1012A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703144"/>
    <dbReference type="KEGG" id="nph:NP_1012A"/>
    <dbReference type="eggNOG" id="arCOG04177">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_181948_4_0_2"/>
    <dbReference type="OrthoDB" id="65887at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1620.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein-like L39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1620.10">
        <property type="entry name" value="Ribosomal protein L39e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00629">
        <property type="entry name" value="Ribosomal_eL39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000077">
        <property type="entry name" value="Ribosomal_eL39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023626">
        <property type="entry name" value="Ribosomal_eL39_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002316">
        <property type="entry name" value="PRK01242.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00832">
        <property type="entry name" value="Ribosomal_L39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48662">
        <property type="entry name" value="Ribosomal protein L39e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="50" mass="6115" checksum="57A5B2F75CC9D543" modified="2017-05-10" version="1">MGKKSKAKKKRLSKLERQNSRVPAWVIMKTDRDTMRNPKRRNWRRSDTDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUI0</accession>
    <name>A0A1U7EUI0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">threonine ammonia-lyase</fullName>
            <ecNumber evidence="3">4.3.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ilvA</name>
        <name evidence="8" type="synonym">tdcB</name>
        <name evidence="8" type="ordered locus">NP_1076A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the serine/threonine dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.19" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322265.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUI0"/>
    <dbReference type="STRING" id="348780.NP_1076A"/>
    <dbReference type="EnsemblBacteria" id="CAI48629">
        <property type="protein sequence ID" value="CAI48629"/>
        <property type="gene ID" value="NP_1076A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702864"/>
    <dbReference type="KEGG" id="nph:NP_1076A"/>
    <dbReference type="eggNOG" id="arCOG01431">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021152_4_1_2"/>
    <dbReference type="OrthoDB" id="9915at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:threonine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006565">
        <property type="term" value="P:L-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04886">
        <property type="entry name" value="ACT_ThrD-II-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01562">
        <property type="entry name" value="Thr-dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000007">
        <property type="entry name" value="L-threonine dehydratase catabolic TdcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000005">
        <property type="entry name" value="Threonine dehydratase catabolic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044561">
        <property type="entry name" value="ACT_ThrD-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050147">
        <property type="entry name" value="Ser/Thr_Dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000634">
        <property type="entry name" value="Ser/Thr_deHydtase_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005789">
        <property type="entry name" value="Thr_deHydtase_catblc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01127">
        <property type="entry name" value="ilvA_1Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078:SF6">
        <property type="entry name" value="L-THREONINE DEHYDRATASE CATABOLIC TDCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00165">
        <property type="entry name" value="DEHYDRATASE_SER_THR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="7">
        <location>
            <begin position="327"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI48629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="402" mass="42690" checksum="E70958710B129170" modified="2017-05-10" version="1">MLELADVTAARPRVAETARQTPLEHSTTFSKRTGADVRLKLENFQRTGSFKIRGATNKVALLEAADREAGVVAASAGNHAQGVALAASKAGVDAVIVMPEHAPISKINATRSYGAEVRLHGIDYDAAAERAHEIEREEGRTFVHAFDDPAVMAGQGTIGLEIVEQCPDVETVVVPIGGGGLIAGVATAVKEQTDARVVGVQAEGAASAADSLEKGEVVERDAVDTIADGIATRKVGDRTFDVIAERVDEVVTVSDDEIAHALTALLERSKTLVEGAGAVPLAAILERRFDYADDETIVPALCGGNIDLNVLTTVITRGLVERGRFLRIKTVLKDRPGALQELVGLLVDHRTNIYAIEHDRASKDIAMNAAEVELDLETRGHDHVVELLDALEDAGYAVEVLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUP7</accession>
    <name>A0A1U7EUP7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1208A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322331.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUP7"/>
    <dbReference type="STRING" id="348780.NP_1208A"/>
    <dbReference type="EnsemblBacteria" id="CAI48695">
        <property type="protein sequence ID" value="CAI48695"/>
        <property type="gene ID" value="NP_1208A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701083"/>
    <dbReference type="KEGG" id="nph:NP_1208A"/>
    <dbReference type="eggNOG" id="arCOG01175">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069300_1_0_2"/>
    <dbReference type="OrthoDB" id="49711at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055549">
        <property type="entry name" value="DUF7125"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23442">
        <property type="entry name" value="DUF7125"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="187" mass="20428" checksum="E813816976481D38" modified="2017-05-10" version="1">MYPTGLPELDRHLGGGVPPGTLVALTAPPNTQSERLLERLAAANETRYVTTLRDAARVRDRLPEASVLSATPDDLLFDPETYLSIPSSGCLVVDSVTELERDTDTYREFLETAARETAESDGIVVLHAHEAEPNPPERWLTLARADMTVQLSLEVSSDAVTNRLAVTKHRGGAVPEKPMQVRLSEDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUR9</accession>
    <name>A0A1U7EUR9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Xanthine/uracil permease family transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">uraA</name>
        <name evidence="8" type="ordered locus">NP_1252A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family. Azg-like subfamily.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EnsemblBacteria" id="CAI48717">
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        <property type="gene ID" value="NP_1252A"/>
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    <dbReference type="KEGG" id="nph:NP_1252A"/>
    <dbReference type="eggNOG" id="arCOG02807">
        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_024508_0_1_2"/>
    <dbReference type="OrthoDB" id="27788at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:purine nucleobase transmembrane transporter activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Azg-like"/>
    </dbReference>
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        <property type="entry name" value="NCS2"/>
    </dbReference>
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    </dbReference>
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            <end position="45"/>
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    </feature>
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            <end position="135"/>
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    </feature>
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        <location>
            <begin position="184"/>
            <end position="205"/>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="230"/>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="304"/>
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            <begin position="324"/>
            <end position="346"/>
        </location>
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        <location>
            <begin position="358"/>
            <end position="379"/>
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    </feature>
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            <end position="405"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <begin position="455"/>
            <end position="474"/>
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    </feature>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUS5</accession>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ordered locus">NP_1286A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
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            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
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            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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    <dbReference type="EnsemblBacteria" id="CAI48734">
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    <dbReference type="KEGG" id="nph:NP_1286A"/>
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    <dbReference type="HOGENOM" id="CLU_2284981_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
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        <property type="entry name" value="DUF7091"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="102" mass="11623" checksum="9F7D5E952163E583" modified="2017-05-10" version="1">MLAFLLPRVSNRRRLERLLRTKLNDAGKQFAEAQRAYTDAKATSLAELPTDESGRARLVCRRHAEKRAVRLDAEGRPACFDADHPDCQGCLEDIRDGRVETW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUT6</accession>
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        <submittedName>
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        </submittedName>
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    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
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            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <property type="entry name" value="Bacterial XPD DNA helicase, FeS cluster domain"/>
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    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
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        <property type="entry name" value="Helic_SF1/SF2_ATP-bd_DinG/Rad3"/>
    </dbReference>
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        <property type="entry name" value="Helicase-like_DEXD_c2"/>
    </dbReference>
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        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010614">
        <property type="entry name" value="RAD3-like_helicase_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042493">
        <property type="entry name" value="XPD_DNA_FeS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="DNA REPAIR DEAD HELICASE RAD3/XP-D SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472:SF34">
        <property type="entry name" value="REGULATOR OF TELOMERE ELONGATION HELICASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06733">
        <property type="entry name" value="DEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00488">
        <property type="entry name" value="DEXDc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51193">
        <property type="entry name" value="HELICASE_ATP_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="6"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="116"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAI48744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="722" mass="81168" checksum="FFC3F91461884CDF" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUU9</accession>
    <name>A0A1U7EUU9_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">uroporphyrinogen-III C-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.107</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">sirA</name>
        <name evidence="9" type="ordered locus">NP_1328A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.107" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322390.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUU9"/>
    <dbReference type="STRING" id="348780.NP_1328A"/>
    <dbReference type="EnsemblBacteria" id="CAI48755">
        <property type="protein sequence ID" value="CAI48755"/>
        <property type="gene ID" value="NP_1328A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702811"/>
    <dbReference type="KEGG" id="nph:NP_1328A"/>
    <dbReference type="eggNOG" id="arCOG00644">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011276_7_0_2"/>
    <dbReference type="OrthoDB" id="24444at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004851">
        <property type="term" value="F:uroporphyrin-III C-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019354">
        <property type="term" value="P:siroheme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11642">
        <property type="entry name" value="SUMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.950.10:FF:000001">
        <property type="entry name" value="Siroheme synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1010.10:FF:000001">
        <property type="entry name" value="Siroheme synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006366">
        <property type="entry name" value="CobA/CysG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050161">
        <property type="entry name" value="Siro_Cobalamin_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003043">
        <property type="entry name" value="Uropor_MeTrfase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01469">
        <property type="entry name" value="cobA_cysG_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004790">
        <property type="entry name" value="PRK06136.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45790:SF3">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT UROPORPHYRINOGEN III METHYLTRANSFERASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45790">
        <property type="entry name" value="SIROHEME SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00839">
        <property type="entry name" value="SUMT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00840">
        <property type="entry name" value="SUMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="8">
        <location>
            <begin position="4"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI48755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="260" mass="27701" checksum="408E4BCC9FE06850" modified="2017-05-10" version="1">MTGMVYLVGSGPGDPELLTVKARRLIDEADVVLHDKLPGPEILESIPEEKREDVGKRAGGEWTPQEYTNRRLVELAEDGQTVVRLKGGDPFVFGRGGEEAEHLAANDIPFEYVPGVTSAIAGAGVAGIPVTHRDHTSSVSFVTGHEDPTKDESAIGWTALANTGGTLVVLMGVGKLPLYTDELLAAGMDSDTPVALVERATWPDQRVATGTLDTIVDVRDEADIEPPAITVIGDVAATRARVREFLVSGHEDAGTEDEDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUX0</accession>
    <name>A0A1U7EUX0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ABC-type transport system ATP-binding protein (Probable substrate biotin)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">bioM</name>
        <name evidence="11" type="synonym">abc21a</name>
        <name evidence="11" type="ordered locus">NP_1396A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Probably part of an ABC transporter complex. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322424.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUX0"/>
    <dbReference type="STRING" id="348780.NP_1396A"/>
    <dbReference type="EnsemblBacteria" id="CAI48789">
        <property type="protein sequence ID" value="CAI48789"/>
        <property type="gene ID" value="NP_1396A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702429"/>
    <dbReference type="KEGG" id="nph:NP_1396A"/>
    <dbReference type="eggNOG" id="arCOG00202">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="OrthoDB" id="35850at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03225">
        <property type="entry name" value="ABC_cobalt_CbiO_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000224">
        <property type="entry name" value="Energy-coupling factor transporter ATP-binding protein EcfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015856">
        <property type="entry name" value="ABC_transpr_CbiO/EcfA_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050095">
        <property type="entry name" value="ECF_ABC_transporter_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43553:SF24">
        <property type="entry name" value="ENERGY-COUPLING FACTOR TRANSPORTER ATP-BINDING PROTEIN ECFA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43553">
        <property type="entry name" value="HEAVY METAL TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="2"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI48789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="232" mass="24416" checksum="E28E19E2D32D63FE" modified="2017-05-10" version="1">MISVSDLVYRYDGTTAVDGVSLQIEDGEFVVIAGANGSGKTTLVRHFNGLYEPDSGEVRVDGQPVAENPVAARTAVGMVFQNPRDCFVAATVGADVAFGPENLGLSHEDIDARIERALAAVGMADAREVRIDTLSGGQQARVAIAGALAMEPSHLVLDEPFAGLDWPARTGVLDRLEALSADGTSVIVVTHDLRDLMDRTDRIVALADGEIVLDGPPSAVAEELPNVDVRPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV05</accession>
    <name>A0A1U7EV05_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC-type transport system permease protein (Probable substrate branched-chain amino acids)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">livH3</name>
        <name evidence="10" type="synonym">abc07p1</name>
        <name evidence="10" type="ordered locus">NP_1466A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322459.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV05"/>
    <dbReference type="STRING" id="348780.NP_1466A"/>
    <dbReference type="EnsemblBacteria" id="CAI48824">
        <property type="protein sequence ID" value="CAI48824"/>
        <property type="gene ID" value="NP_1466A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702384"/>
    <dbReference type="KEGG" id="nph:NP_1466A"/>
    <dbReference type="eggNOG" id="arCOG01270">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039929_2_1_2"/>
    <dbReference type="OrthoDB" id="43815at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06582">
        <property type="entry name" value="TM_PBP1_LivH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052157">
        <property type="entry name" value="BCAA_transport_permease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11795:SF450">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11795">
        <property type="entry name" value="BRANCHED-CHAIN AMINO ACID TRANSPORT SYSTEM PERMEASE PROTEIN LIVH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="149"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="229"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="260"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI48824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="297" mass="30954" checksum="9AD8429888E6DBBE" modified="2017-05-10" version="1">MAIETFAGLFVTAAMISAVYALIAIGFTMIFGVGGVLNLAHGGLIMIGAYAYLVVTSTSTVPSIVIPPVAGLVVAIVVAALASFALYKGLVEFIEDDVIITFLATVVVAVILAELMELIFSANPRSLTPFVPGSTALPAIGVRIQHIEIAGFVLSWVAIGALWYYVTKTDAGRSILATSMTQRGAKLTGVDIAAVSARTWLIAGGLAGLAGVFLGTLQQTSPLMWLDPLALAFIIVVIGGIGSIKGSLVAAYFIGFLETYTVGLLGPEFRGVFALIIVAAVILIRPEGLYGREFVDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV45</accession>
    <name>A0A1U7EV45_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">VKGC domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">NP_1578A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322514.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV45"/>
    <dbReference type="STRING" id="348780.NP_1578A"/>
    <dbReference type="EnsemblBacteria" id="CAI48880">
        <property type="protein sequence ID" value="CAI48880"/>
        <property type="gene ID" value="NP_1578A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701191"/>
    <dbReference type="KEGG" id="nph:NP_1578A"/>
    <dbReference type="eggNOG" id="arCOG06405">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022162_0_0_2"/>
    <dbReference type="OrthoDB" id="327281at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011020">
        <property type="entry name" value="HTTM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053934">
        <property type="entry name" value="HTTM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052964">
        <property type="entry name" value="Sporulation_signal_mat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39535:SF2">
        <property type="entry name" value="HTTM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39535">
        <property type="entry name" value="SPORULATION-DELAYING PROTEIN SDPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05090">
        <property type="entry name" value="HTTM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00752">
        <property type="entry name" value="HTTM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="165"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="271"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="327"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="HTTM-like" evidence="6">
        <location>
            <begin position="18"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="491" mass="53038" checksum="2FA6140A03971F73" modified="2017-05-10" version="1">MSPPPTAESDTSGWLRHRLGIDARALAAFRVAVALLVIADLLFRARGIPAFYTDAGTLPRGALAAEYPWLAVASIHTVTGSAVGQTVLFLLSGLVALVLAVGYHTRLATAATAALHASLHARNPYLLNGGDALLVVLLALAVFLPLGERWSVDALRRDRQPRQYVASLATAVVLAQLVVVYLFNAVFKYQSEAWMRGEAVQYALGLEQFAVWLGPMLAAFPTVLVAVNWSWVLLLTAAPLLVVLTGWRRVAVVAAFAAAHFGMFVTMRLGLFPAVVWAILLLYLPSTVWDRIEAILGGRQVVSRTAVERLPSLPAPPKRLREGGRRAAVVVLVVVLVVTVVWPMGAAVDTETDRVDAPDYAWTLFSPTPPTETRWFVAPTTFETGETVDAFDGSAVTWDPPPDAAKTYASTLWHRYLNEMRFASDSERQPLGAYLCQQAAAESGEAVTQLTVYAVERPVEAVGGGEATRVELLSTECNVDTASVAETRNLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV69</accession>
    <name>A0A1U7EV69_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Amine oxidase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1630A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322540.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV69"/>
    <dbReference type="STRING" id="348780.NP_1630A"/>
    <dbReference type="EnsemblBacteria" id="CAI48906">
        <property type="protein sequence ID" value="CAI48906"/>
        <property type="gene ID" value="NP_1630A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702635"/>
    <dbReference type="KEGG" id="nph:NP_1630A"/>
    <dbReference type="eggNOG" id="arCOG01523">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039679_0_0_2"/>
    <dbReference type="OrthoDB" id="202781at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42841">
        <property type="entry name" value="AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="1">
        <location>
            <begin position="10"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="418" mass="44558" checksum="310C0C5E2C1120A3" modified="2017-05-10" version="1">MHVVVAGGGLAGLVAARHLAEAGAEVELFERNERVGGRVRSRTVDGFTIDRGFQVLFTGYPAAQRELDFDALDLRAFAPGATIAAPNHRSTLADPLGDPGAAVPTLLNRDVRLSDKLRLFRLQRRLKRRAVDAIFEGDDRTIREYLAAEGFSTAFVERFAEPFYGGITLDRELETSSHVFEYTFKMLSEGRTVVPAEGMGAIPAQLRTAAETAGATVRTGTAVTDITTDDGEVTVRTDDGTQTADAAVVATDPNAAAELTDIETPDAGLGCVTVHCSLPERQALDTGSRILLNADDDEPNTIAPMSAAAPAYAPEGSQLLSATFLGTADRDDAALGETVREALAAWYPENRFAELDVVAVDRVPFAQFAQPPGFRADLPSPDAPDGPVVLAGDYTRWSSIQGALESGRQAAEAVLEQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV80</accession>
    <name>A0A1U7EV80_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1676A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV80"/>
    <dbReference type="EnsemblBacteria" id="CAI48929">
        <property type="protein sequence ID" value="CAI48929"/>
        <property type="gene ID" value="NP_1676A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701220"/>
    <dbReference type="KEGG" id="nph:NP_1676A"/>
    <dbReference type="eggNOG" id="arCOG04602">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2327184_0_0_2"/>
    <dbReference type="OrthoDB" id="203436at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055535">
        <property type="entry name" value="DUF7111"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23423">
        <property type="entry name" value="DUF7111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="89" mass="9697" checksum="F44A9BF6698B8EE1" modified="2017-05-10" version="1">MSTVEEGDITAQYRETDEERVLEFEAGGKTAAVAQNIDGYAMLKVRPSADGDELERYYGFDMAIDHAAELLDVAPSELPIPEPAADMGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVA4</accession>
    <name>A0A1U7EVA4_NATPD</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1660A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322554.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVA4"/>
    <dbReference type="SMR" id="A0A1U7EVA4"/>
    <dbReference type="STRING" id="348780.NP_1660A"/>
    <dbReference type="EnsemblBacteria" id="CAI48921">
        <property type="protein sequence ID" value="CAI48921"/>
        <property type="gene ID" value="NP_1660A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701215"/>
    <dbReference type="KEGG" id="nph:NP_1660A"/>
    <dbReference type="eggNOG" id="arCOG08193">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131317_0_0_2"/>
    <dbReference type="OrthoDB" id="319635at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="175" mass="19721" checksum="986F6F0FD31AACA9" modified="2017-05-10" version="1">MDAATVETYLDSYAETQGVLPERLESFGERYREQGYLTRDQLYDIAYESSTRSAYHVESNPEDRCREVTRNVRAVDGDFSKIQLLSGLSGFKAPTASCVLTALDSTTHAVVDTRVWASLERFDYLEGRKESFDAADYVRMIEPIRTIAAKTGYSTADVGYALFAYDAEHREGTLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVD8</accession>
    <name>A0A1U7EVD8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Malate dehydrogenase (Oxaloacetate-decarboxylating)</fullName>
            <ecNumber evidence="11">1.1.1.40</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">maeB2</name>
        <name evidence="11" type="synonym">mdh2:pta2</name>
        <name evidence="11" type="ordered locus">NP_1772A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the malic enzymes family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.40" evidence="11"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322610.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVD8"/>
    <dbReference type="STRING" id="348780.NP_1772A"/>
    <dbReference type="EnsemblBacteria" id="CAI48977">
        <property type="protein sequence ID" value="CAI48977"/>
        <property type="gene ID" value="NP_1772A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702905"/>
    <dbReference type="KEGG" id="nph:NP_1772A"/>
    <dbReference type="eggNOG" id="arCOG00853">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012366_0_0_2"/>
    <dbReference type="OrthoDB" id="45556at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004473">
        <property type="term" value="F:malate dehydrogenase (decarboxylating) (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05311">
        <property type="entry name" value="NAD_bind_2_malic_enz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10380:FF:000003">
        <property type="entry name" value="NADP-dependent malic enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000095">
        <property type="entry name" value="NADP-dependent malic enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10750">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10380">
        <property type="entry name" value="Malic enzyme, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051674">
        <property type="entry name" value="Malate_Decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012301">
        <property type="entry name" value="Malic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037062">
        <property type="entry name" value="Malic_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012302">
        <property type="entry name" value="Malic_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045213">
        <property type="entry name" value="Malic_NAD-bd_bact_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012188">
        <property type="entry name" value="ME_PTA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042113">
        <property type="entry name" value="P_AcTrfase_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042112">
        <property type="entry name" value="P_AcTrfase_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002505">
        <property type="entry name" value="PTA_PTB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43237">
        <property type="entry name" value="NADP-DEPENDENT MALIC ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43237:SF4">
        <property type="entry name" value="NADP-DEPENDENT MALIC ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00390">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03949">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01515">
        <property type="entry name" value="PTA_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036684">
        <property type="entry name" value="ME_PTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01274">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00919">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="6 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Malic enzyme N-terminal" evidence="10">
        <location>
            <begin position="16"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Malic enzyme NAD-binding" evidence="9">
        <location>
            <begin position="161"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI48977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="751" mass="81336" checksum="97FEAA823001DF69" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVE7</accession>
    <name>A0A1U7EVE7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1798A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322622.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVE7"/>
    <dbReference type="EnsemblBacteria" id="CAI48990">
        <property type="protein sequence ID" value="CAI48990"/>
        <property type="gene ID" value="NP_1798A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701256"/>
    <dbReference type="KEGG" id="nph:NP_1798A"/>
    <dbReference type="eggNOG" id="ENOG502N5M0">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1727294_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="151" mass="15528" checksum="70069C94DF9FC2A1" modified="2017-05-10" version="1">MNSQLENIGKLVASMLVVGVGIGIGAFFGLEWTFDTIVPQVDPTAQQIISDLGPLIMLFVLFASTPVIGGIIGLIEGRNITYDRQRLLTTMFSCLLGGILVVLISMAIIGTAGEGGGGPEFAVVDLLSIAGLVGISSAFTSGLAMAITGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVF4</accession>
    <name>A0A1U7EVF4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ABC-type transport system periplasmic substrate-binding protein (Probable substrate glutamine/glutamate/polar amino acids)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">abc04p1</name>
        <name evidence="4" type="ordered locus">NP_1774A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322611.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVF4"/>
    <dbReference type="STRING" id="348780.NP_1774A"/>
    <dbReference type="EnsemblBacteria" id="CAI48978">
        <property type="protein sequence ID" value="CAI48978"/>
        <property type="gene ID" value="NP_1774A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702374"/>
    <dbReference type="KEGG" id="nph:NP_1774A"/>
    <dbReference type="eggNOG" id="arCOG01799">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019602_18_2_2"/>
    <dbReference type="OrthoDB" id="30671at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35936:SF17">
        <property type="entry name" value="ARGININE-BINDING EXTRACELLULAR PROTEIN ARTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35936">
        <property type="entry name" value="MEMBRANE-BOUND LYTIC MUREIN TRANSGLYCOSYLASE F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00497">
        <property type="entry name" value="SBP_bac_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Solute-binding protein family 3/N-terminal" evidence="3">
        <location>
            <begin position="57"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="30"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="151"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="42"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="279" mass="29949" checksum="BFD8221F1AFCC3FC" modified="2017-05-10" version="1">MESDPTLDRRSYLSAVGAAGLAAGAAGCVGLDDGNGDGNGDGNDDDSDDDSDDDTRTIVAGTAPGFPPFEFRQDGELLGFDIDLTEAVIERSDYEFGGWETFEFDSLIPALQNGNIDVIAAAMTIEDERREQIAFSDAYYEADQSVLVRSDDDDLPQSREDLSGSIVGAQGGTTGENEVDRLIDDGIIPESDKNVYDNYTLAATDLENGNIDAVVVDVPVARTFSEDLAVEVAFEIETGEEYGFGIRQDDEELVAAVNDGLEAVTDDGTYNDLVAEWFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVG6</accession>
    <name>A0A1U7EVG6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FtsZ family protein, noncanonical</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1848A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322647.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVG6"/>
    <dbReference type="STRING" id="348780.NP_1848A"/>
    <dbReference type="EnsemblBacteria" id="CAI49015">
        <property type="protein sequence ID" value="CAI49015"/>
        <property type="gene ID" value="NP_1848A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701275"/>
    <dbReference type="KEGG" id="nph:NP_1848A"/>
    <dbReference type="eggNOG" id="arCOG02452">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1275361_0_0_2"/>
    <dbReference type="OrthoDB" id="199953at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="216" mass="22709" checksum="666CF47D8CFEAB5E" modified="2017-05-10" version="1">MSSLEQAFSVSGLPVAELDNGTSVLITGDDTEALRTLFARVVAPRNEEAGLVLDTEGDARSLRRSLDSVVSGAGDASTVLTTDVSVSGDDVGIDDLSDVTTIGMSLSNLTAEIQQETDRFRTGVFLCTGICTEVDDMRSVYRFLNTNFLSSLRRNDAIGVCVVDTSADIGTETSSLVAGLDTSFSARIDIESVDSREATLSLSGFNDADGEATVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVN3</accession>
    <name>A0A1U7EVN3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC-type transport system permease protein (Probable substrate urea/short-chain amides)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">urtB</name>
        <name evidence="10" type="synonym">abc10p1</name>
        <name evidence="10" type="ordered locus">NP_2002A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVN3"/>
    <dbReference type="STRING" id="348780.NP_2002A"/>
    <dbReference type="EnsemblBacteria" id="CAI49092">
        <property type="protein sequence ID" value="CAI49092"/>
        <property type="gene ID" value="NP_2002A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_2002A"/>
    <dbReference type="eggNOG" id="arCOG01271">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039929_2_2_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06582">
        <property type="entry name" value="TM_PBP1_LivH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019924">
        <property type="entry name" value="ABC_UrtB_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052157">
        <property type="entry name" value="BCAA_transport_permease"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03622">
        <property type="entry name" value="urea_t_UrtB_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11795:SF447">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11795">
        <property type="entry name" value="BRANCHED-CHAIN AMINO ACID TRANSPORT SYSTEM PERMEASE PROTEIN LIVH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="222"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="256"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI49092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="300" mass="31738" checksum="D8D0B16E098A31A9" modified="2017-05-10" version="1">MPLSPLYDGAALLFQFLDSFAFLVLATVGLAVIFGMMGVINLAHGEFILVGIYGTALTYHAGLPLPVAMVSGVVMTVIFGIIVERLVVQHLYDRLLDSMVATWGLALVVAQLLLITFGSSLDSISTPMGNVAYGPYTSGTYRSVFLPLVALAVLVGLYVLFTRTEFGVKARATIEDPETARAMGIDTDRMYVTTFALGSGLAGLTGALYAPAIGAITPDRGSIFLVEAFVAVVVGGPSVVIGTLSASGLLGFVNAVFSFQLGTFVGLMAMLIVAIIALRVLPDGISGYIEQWRQRRRADQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVP3</accession>
    <name>A0A1U7EVP3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC-type transport system permease protein (Probable substrate heme)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ccmB</name>
        <name evidence="9" type="synonym">abc29p1</name>
        <name evidence="9" type="ordered locus">NP_2036A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CcmB/CycW/HelB family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322738.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVP3"/>
    <dbReference type="STRING" id="348780.NP_2036A"/>
    <dbReference type="EnsemblBacteria" id="CAI49109">
        <property type="protein sequence ID" value="CAI49109"/>
        <property type="gene ID" value="NP_2036A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702452"/>
    <dbReference type="KEGG" id="nph:NP_2036A"/>
    <dbReference type="eggNOG" id="arCOG01328">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079069_0_0_2"/>
    <dbReference type="OrthoDB" id="51643at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015232">
        <property type="term" value="F:heme transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903607">
        <property type="term" value="P:cytochrome c biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003544">
        <property type="entry name" value="Cyt_c_biogenesis_CcmB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30070:SF1">
        <property type="entry name" value="CYTOCHROME C BIOGENESIS B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30070">
        <property type="entry name" value="HEME EXPORTER PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03379">
        <property type="entry name" value="CcmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01414">
        <property type="entry name" value="CCMBBIOGNSIS"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="97"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="137"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI49109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="225" mass="24108" checksum="635E638856E9723D" modified="2017-05-10" version="1">MTGRYLRTVYEVARKDLLVETRSKQTVNSAFVFSLLVIVVFSFVFGEDVGSREALARGALWIAVVFGGVVGMSHAVALEGQNDAIDGLLLAPVDRSAVYLGKVLSTTVFVTGIGLLSLVFVVVFLEYSFEPAALSTLLSVVPLAALGLSAVGVLLSVLMLHSQLQESLLPVLLIPLVVPVLLAGTALTQPDLSDGMTTAWFRVLLAYDGLMLLAGWMTFEYVVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVP8</accession>
    <name>A0A1U7EVP8_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Archaeal Rqc2 homolog aRqcH</fullName>
            <shortName evidence="1">aRqcH</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rqcH</name>
        <name evidence="4" type="ordered locus">NP_2070A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probably part of the ribosome quality control system (RQC). May mediate the addition of alanine residues (Ala tailing) to incompletely synthesized nascent chains from stalled ribosomes, leading to their degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Associates with stalled 50S ribosomal subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NEMF family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322755.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVP8"/>
    <dbReference type="STRING" id="348780.NP_2070A"/>
    <dbReference type="EnsemblBacteria" id="CAI49126">
        <property type="protein sequence ID" value="CAI49126"/>
        <property type="gene ID" value="NP_2070A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701342"/>
    <dbReference type="KEGG" id="nph:NP_2070A"/>
    <dbReference type="eggNOG" id="arCOG01695">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003612_2_1_2"/>
    <dbReference type="OrthoDB" id="10943at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.310.10">
        <property type="entry name" value="ibrinogen binding protein from staphylococcus aureus domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00844_A">
        <property type="entry name" value="RqcH_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008532">
        <property type="entry name" value="NFACT_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051608">
        <property type="entry name" value="RQC_Subunit_NEMF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043681">
        <property type="entry name" value="RqcH_archaeal"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041120">
        <property type="entry name" value="RqcH_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15239">
        <property type="entry name" value="NUCLEAR EXPORT MEDIATOR FACTOR NEMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15239:SF6">
        <property type="entry name" value="RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT NEMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05670">
        <property type="entry name" value="NFACT-R_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05833">
        <property type="entry name" value="NFACT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="1" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="NFACT RNA-binding" evidence="3">
        <location>
            <begin position="491"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="439"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="309"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="456"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="699" mass="78725" checksum="3A6C7C4E1BB8F4FD" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVQ7</accession>
    <name>A0A1U7EVQ7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7260 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2078A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322758.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVQ7"/>
    <dbReference type="STRING" id="348780.NP_2078A"/>
    <dbReference type="EnsemblBacteria" id="CAI49130">
        <property type="protein sequence ID" value="CAI49130"/>
        <property type="gene ID" value="NP_2078A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701345"/>
    <dbReference type="KEGG" id="nph:NP_2078A"/>
    <dbReference type="eggNOG" id="arCOG06167">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076271_1_0_2"/>
    <dbReference type="OrthoDB" id="213880at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055684">
        <property type="entry name" value="DUF7260"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23921">
        <property type="entry name" value="DUF7260"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7260" evidence="2">
        <location>
            <begin position="6"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="264" mass="29219" checksum="32DB3E5929D02033" modified="2017-05-10" version="1">MALTNHLDRATGRVADEQDVIADERRAYERFRTTVETTATDAHSTTSAASTGAGAAAVGLQRRSRPDRGCSEIRAAFAETVRPYSTDDIDREESVIETLGAELGDSIAAALSPETTHEFTPQLKTAILAAVDDRHAELAVMERALTREAESLQTAVEAYDDITDRLLERNETSLFEFGFPGLRQRHEALAADRETCERLLERRQELLASTTSRGGDAGINHRTLVRYLYQSQPATYPALSTLVQLDSTLADCQRTLRDHLTRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVW9</accession>
    <name>A0A1U7EVW9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HEAT-PBS family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2232A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVW9"/>
    <dbReference type="STRING" id="348780.NP_2232A"/>
    <dbReference type="EnsemblBacteria" id="CAI49207">
        <property type="protein sequence ID" value="CAI49207"/>
        <property type="gene ID" value="NP_2232A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_2232A"/>
    <dbReference type="eggNOG" id="arCOG02966">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053271_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004155">
        <property type="entry name" value="PBS_lyase_HEAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12697:SF5">
        <property type="entry name" value="DEOXYHYPUSINE HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12697">
        <property type="entry name" value="PBS LYASE HEAT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13646">
        <property type="entry name" value="HEAT_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00567">
        <property type="entry name" value="EZ_HEAT"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="389"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="409" mass="45394" checksum="943ED93D71C54D9B" modified="2017-05-10" version="1">MLEPPVDSSDIPELCRLLRGGDTPTVRQAAANALGRISTSSDGDEVEQALDELMGAVLRDDSEEVRGEAIDALYRYGSEHIDRLATRLAEAVRTRGSETPGAFFRRWLGSDVPQFRLVAAAAMATRADERVADELEAAFTDSDRRVQARALEAYGNVGDAAAVEPVRQALETDDPMVRRAAATALANIGTEEALEAMLPLAQATENRLRRLTVEQLHRLDRRQSAVVLVGALDDRSETVRRRAMVSLILLYTSSRPVEPDTVRDYLIKERNHEELVDLARLLSNIVADDGEGTSRESQTVKRHAVWLLGEIAGTLDDEEVHCWLADSLRTPDRAATEMAAAYLRQFEGEKLESKLRSMSRDADCSAETRTIASSVLERIKEDMAKDTEKHDVDYTYVRQPSDYTEKHGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW41</accession>
    <name>A0A1U7EW41_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transport protein (Probable substrate cationic amino acids)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cat8</name>
        <name evidence="8" type="synonym">tp04</name>
        <name evidence="8" type="ordered locus">NP_2382A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322908.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW41"/>
    <dbReference type="SMR" id="A0A1U7EW41"/>
    <dbReference type="STRING" id="348780.NP_2382A"/>
    <dbReference type="EnsemblBacteria" id="CAI49282">
        <property type="protein sequence ID" value="CAI49282"/>
        <property type="gene ID" value="NP_2382A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703281"/>
    <dbReference type="KEGG" id="nph:NP_2382A"/>
    <dbReference type="eggNOG" id="arCOG00009">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_15_12_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050367">
        <property type="entry name" value="APC_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770">
        <property type="entry name" value="AMINO ACID TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770:SF11">
        <property type="entry name" value="INNER MEMBRANE TRANSPORT PROTEIN YBAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="136"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="246"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="290"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="367"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="408"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="432"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="7">
        <location>
            <begin position="23"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="458" mass="46596" checksum="09A22EDB131B575D" modified="2017-05-10" version="1">MPPEQQAVTSESDLDRTVGLAGALTIGVGTMIGAGIFVFPGLAAGRAGPAAALSFAIGAVIALLVALPTAELATAMPKSGGGYYFISRSLGALPGAVVGVSLGFGLVFASAFYLVGFGEYAVSILEAAGVVGEPPVAVGPITAAALLGIVAGIGLTAISILGTENTETLQNAVVGLLLAILAVFLSASGLELLGVVGDREPATGFAPAGYEPVLTTAALVFTSYLGFAQIATVGEDIVEPDRNLPLAMVGSVVLVGILYVVTLLIAGSTFAPETIGPMGETAIVEVARVYLGPLGAASVLLAGLLATVSSANASILSASRTLYALSADRLAPPQAAELSHRYGTPHFALSFAGGVTVLLVAAGRTEVLAEVASFLHLVMYGLMCLALLKLRHEEPAWYQPSFECPGYPAVPIAGGLASFGLIYYMQPISRRLGAVIAVVAVGWYFLYAEDVSLREELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW43</accession>
    <name>A0A1U7EW43_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable secreted glycoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2396A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322915.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW43"/>
    <dbReference type="EnsemblBacteria" id="CAI49289">
        <property type="protein sequence ID" value="CAI49289"/>
        <property type="gene ID" value="NP_2396A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701434"/>
    <dbReference type="KEGG" id="nph:NP_2396A"/>
    <dbReference type="eggNOG" id="arCOG06295">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1472134_0_0_2"/>
    <dbReference type="OrthoDB" id="387351at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="183" mass="19335" checksum="28014B32A7DAB310" modified="2017-05-10" version="1">METQVSRRRLLQAGGVGAAASFAGCGYMDTEDGGAPADMSGQGPHTVAIGVAIDQMEMQAAEQEFEGIQQEAQEDFEAGEIDQQEASDMIEEAQGELQDTQRQLLQDAIDGVEAHAEGTEALSVLDSELEFGLVLVEGSGDAIVGTLASEHVQAIVPEDEFEAIQGPQGEPEPEPEPEPEPEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW53</accession>
    <name>A0A1U7EW53_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">3-hydroxy-3-methylglutaryl coenzyme A reductase</fullName>
            <shortName evidence="5">HMG-CoA reductase</shortName>
            <ecNumber evidence="5">1.1.1.34</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">hmgA1</name>
        <name evidence="6" type="synonym">mvaA1</name>
        <name evidence="6" type="ordered locus">NP_2422A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>(R)-mevalonate + 2 NADP(+) + CoA = (3S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15989"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36464"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.34"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the HMG-CoA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.34" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322928.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW53"/>
    <dbReference type="STRING" id="348780.NP_2422A"/>
    <dbReference type="EnsemblBacteria" id="CAI49302">
        <property type="protein sequence ID" value="CAI49302"/>
        <property type="gene ID" value="NP_2422A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702955"/>
    <dbReference type="KEGG" id="nph:NP_2422A"/>
    <dbReference type="eggNOG" id="arCOG04260">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001734_2_2_2"/>
    <dbReference type="OrthoDB" id="10981at2157"/>
    <dbReference type="UniPathway" id="UPA00058">
        <property type="reaction ID" value="UER00103"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004420">
        <property type="term" value="F:hydroxymethylglutaryl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015936">
        <property type="term" value="P:coenzyme A metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00643">
        <property type="entry name" value="HMG-CoA_reductase_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.420:FF:000001">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl coenzyme A reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.770.10">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl-coenzyme A Reductase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.420">
        <property type="entry name" value="Hydroxymethylglutaryl-CoA reductase, class I/II, NAD/NADP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002202">
        <property type="entry name" value="HMG_CoA_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023074">
        <property type="entry name" value="HMG_CoA_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023076">
        <property type="entry name" value="HMG_CoA_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004554">
        <property type="entry name" value="HMG_CoA_Rdtase_eu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009023">
        <property type="entry name" value="HMG_CoA_Rdtase_NAD(P)-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009029">
        <property type="entry name" value="HMG_CoA_Rdtase_sub-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00533">
        <property type="entry name" value="HMG_CoA_R_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572:SF24">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00368">
        <property type="entry name" value="HMG-CoA_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00071">
        <property type="entry name" value="HMGCOARDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55035">
        <property type="entry name" value="NAD-binding domain of HMG-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56542">
        <property type="entry name" value="Substrate-binding domain of HMG-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00066">
        <property type="entry name" value="HMG_COA_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00318">
        <property type="entry name" value="HMG_COA_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50065">
        <property type="entry name" value="HMG_COA_REDUCTASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0521">NADP</keyword>
    <keyword evidence="3 5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI49302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="402" mass="40483" checksum="F7A1EC547212E30C" modified="2017-05-10" version="1">MDTDALVDAVRDGELRLHELEAHADADTAAAARRRIVADAADTSLETVGEYAFPADDAEPNIENMVGAAQVPMGVVGPLAVDGDAIDGEPYLPLATTEGALVASVNRGCASMTAAGGATARVLKNAMTRAPVFRVAGVAEASETAAWVRDNVESLASAAEATTSHGELRDVTPYVVGDNVFLRFAYDTKDAMGMNMATIATEAACEVVEAETPAELVALSGNLCSDKKPAAVNSVEGRGRTVAADVVLPGSVVEEYFGTTPAAIAEANTRKNLVGSAKAGSLGFNAHAANTVAAAFLATGQDIAQVVEGANAITTADVRDGDLYASLTLASLEVGTVGGGTKLPTQAEALDVVGVRGGGDPAGSNADALAEAIATAALGGELSLLGALASNHLASAHEELGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWB9</accession>
    <name>A0A1U7EWB9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acetyl-CoA C-acyltransferase</fullName>
            <ecNumber evidence="4">2.3.1.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">acaB1</name>
        <name evidence="4" type="ordered locus">NP_2606A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.16" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323019.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWB9"/>
    <dbReference type="STRING" id="348780.NP_2606A"/>
    <dbReference type="EnsemblBacteria" id="CAI49394">
        <property type="protein sequence ID" value="CAI49394"/>
        <property type="gene ID" value="NP_2606A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702459"/>
    <dbReference type="KEGG" id="nph:NP_2606A"/>
    <dbReference type="eggNOG" id="arCOG01278">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035425_4_0_2"/>
    <dbReference type="OrthoDB" id="167534at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003988">
        <property type="term" value="F:acetyl-CoA C-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00829">
        <property type="entry name" value="SCP-x_thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055140">
        <property type="entry name" value="Thiolase_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004720">
        <property type="entry name" value="PRK06064.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870">
        <property type="entry name" value="ACETYL-COA C-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870:SF1">
        <property type="entry name" value="NON-SPECIFIC LIPID-TRANSFER PROTEIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22691">
        <property type="entry name" value="Thiolase_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="1" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="2">
        <location>
            <begin position="13"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="3">
        <location>
            <begin position="241"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="393" mass="40868" checksum="DF2C1A408B805FCE" modified="2017-05-10" version="1">MPDAYLVGAGQSDYGAFPDESYRSLFRTAFEAAAESVPEGFDKDAVDEAFIGTLGVGGRQLGLSGPAVTEHVGLDGVPCTRVENACAASGFAVRQAVQAVKSGMADVVLAGGFEVMSDMSSDATKYWLGVSGETEWERLSGTTFSGVYAQMASAHMDRYGTTGEQLSRVAVKNHANGAKNPHAQLGFECSLDDAQSAPMVADPLNLYHCCPTSDGAACALVVSEAVVDDYTDDPIRVAGVGAGSDNVGLFDRETYTAVPASQRAAERAYEMAGIEPTDLDFAEVHDCFAIAELLAYEDLGFCERGESGSFIESGATELDGELPVNTSGGLKSKGHPIGATGAGQVVEAFKQLSGKAGDRQVDSPHRGLTHNVGGSGGAAIVHVFEKEQEVSAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWD6</accession>
    <name>A0A1U7EWD6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Acyl-CoA dehydrogenase</fullName>
            <ecNumber evidence="10">1.3.8.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">acd2</name>
        <name evidence="10" type="ordered locus">NP_2628A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.-" evidence="10"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323030.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWD6"/>
    <dbReference type="STRING" id="348780.NP_2628A"/>
    <dbReference type="EnsemblBacteria" id="CAI49405">
        <property type="protein sequence ID" value="CAI49405"/>
        <property type="gene ID" value="NP_2628A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702477"/>
    <dbReference type="KEGG" id="nph:NP_2628A"/>
    <dbReference type="eggNOG" id="arCOG01707">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_0_2_2"/>
    <dbReference type="OrthoDB" id="275197at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000004">
        <property type="entry name" value="Acyl-CoA dehydrogenase FadE25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000001">
        <property type="entry name" value="Acyl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF12">
        <property type="entry name" value="ISOVALERYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016578">
        <property type="entry name" value="HsaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0274">FAD</keyword>
    <keyword evidence="3 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 6 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="9">
        <location>
            <begin position="6"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="8">
        <location>
            <begin position="121"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="7">
        <location>
            <begin position="228"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI49405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="379" mass="41182" checksum="11881116BDF44ABD" modified="2017-05-10" version="1">MAFSLSDEHEAIRDAVREFGENEIKPVAEEHDRKGEYPLELRKKAAEYDFVAPSIPMEYDGAGMDKISSTIVTEELWRADPGIGSAIGSAGFGTNMILEFGDDWMKEEWLPKVANGETASCSMISEPAHGSNVAGIETVAEEDGDGYVLNGSKMWITNGTVADVGVLMAKTSPGEGHQGITAFLVEMDRDGVTTDKIDNKLGIRASDLAEVVVDDVRVPEENVIGEVDKGFYQLMEFFASGRTSVAAQAVGAAQGALDAAIEYADQREQFGQKINEFQAIEHKLAEMATQVEAARSLTYRAATEVEQNNQSVAAQFSSMAKLFASEMAVDVADEGIQVHGGSGYVTDYPAERYYRDARITKIYEGTSEIQKNIIADQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWH9</accession>
    <name>A0A1U7EWH9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ArCOG06342 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2732A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323082.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWH9"/>
    <dbReference type="STRING" id="348780.NP_2732A"/>
    <dbReference type="EnsemblBacteria" id="CAI49457">
        <property type="protein sequence ID" value="CAI49457"/>
        <property type="gene ID" value="NP_2732A"/>
    </dbReference>
    <dbReference type="GeneID" id="43500090"/>
    <dbReference type="KEGG" id="nph:NP_2732A"/>
    <dbReference type="eggNOG" id="arCOG06342">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1178156_0_0_2"/>
    <dbReference type="OrthoDB" id="178099at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="235" mass="24713" checksum="711F7E83990B08C4" modified="2017-05-10" version="1">MGDDSTPVRAVFELQRTAIEGTQETLERGIEVQRRLNETAESNTDAVSDIAEGGGDVVRAGIDTSLDAAETAIPEDGDIIGDARETIDKRLDAAGDVRRDVADGVESGLEESFELTDELLAAALDVVDGGAETALEANEAAESQVADAVEELDETMDDLQAAVEDRLERTAEETESDDADSDHTTIEIEDLSEDTEESPEAETDPAAASGQESDSESAADDSATTVADTDADTET</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWQ3</accession>
    <name>A0A1U7EWQ3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2886A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323159.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWQ3"/>
    <dbReference type="STRING" id="348780.NP_2886A"/>
    <dbReference type="EnsemblBacteria" id="CAI49534">
        <property type="protein sequence ID" value="CAI49534"/>
        <property type="gene ID" value="NP_2886A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701584"/>
    <dbReference type="KEGG" id="nph:NP_2886A"/>
    <dbReference type="eggNOG" id="arCOG04662">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070751_2_0_2"/>
    <dbReference type="OrthoDB" id="313603at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014509">
        <property type="entry name" value="YjdF-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09997">
        <property type="entry name" value="DUF2238"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="207" mass="22732" checksum="E79B3E50DE5DD2B5" modified="2017-05-10" version="1">MKLPRPSVRNQRRLTAGMRLLLAGIVVYGLVYGQPKAITNGLLSLAITFVPAVMERNYGIPLDPWLGLWITLAVFLHTMGSAGLYGHFEWWDHLTHAMSASLVAGAGYTFARAVDLHNDRIHIPRRFFFVYVLVVVLAFGVVWELFEFGLDVAADATGIEMPLAQHGLDDTVRDLIFNSLGALAVATFGQAHLSGVAEKIQTSLTAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWQ8</accession>
    <name>A0A1U7EWQ8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2880A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323156.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWQ8"/>
    <dbReference type="STRING" id="348780.NP_2880A"/>
    <dbReference type="EnsemblBacteria" id="CAI49531">
        <property type="protein sequence ID" value="CAI49531"/>
        <property type="gene ID" value="NP_2880A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701581"/>
    <dbReference type="KEGG" id="nph:NP_2880A"/>
    <dbReference type="eggNOG" id="arCOG09258">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_200899_0_0_2"/>
    <dbReference type="OrthoDB" id="174349at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="71" mass="7371" checksum="F5E3D3F2D61C1940" modified="2017-05-10" version="1">MHQNTFVKLSALAFGLVLVSFIVRGFGGFLVGGETATLISAPPMLVGAGLIVFLTLRSVLAVSGIRPLAEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWT7</accession>
    <name>A0A1U7EWT7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CopG domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2958A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323194.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWT7"/>
    <dbReference type="STRING" id="348780.NP_2958A"/>
    <dbReference type="EnsemblBacteria" id="CAI49570">
        <property type="protein sequence ID" value="CAI49570"/>
        <property type="gene ID" value="NP_2958A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701610"/>
    <dbReference type="KEGG" id="nph:NP_2958A"/>
    <dbReference type="eggNOG" id="arCOG04451">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104048_0_0_2"/>
    <dbReference type="OrthoDB" id="167615at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22235">
        <property type="entry name" value="RHH_CopG_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002145">
        <property type="entry name" value="CopG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01402">
        <property type="entry name" value="RHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribbon-helix-helix protein CopG" evidence="1">
        <location>
            <begin position="3"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="180" mass="20274" checksum="438D8DDD8DE1081B" modified="2017-05-10" version="1">MTRRVTVSLDDDTAAALEQLDDETGAGQSEIVRRALSFYAANYEAATGRQSDALEQYYRMLSSGEHVLLDIDFLHAFLEHLYAGGDPDAEFVAAADRVSDYHASEYASRFESVGELLEWLSFCGFLDVRHEDDGVYHAVFPSEAIQWFMTRFIERSTTDLPGDIETERGVSKLIVTERET</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWU7</accession>
    <name>A0A1U7EWU7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Asparagine synthase (Glutamine-hydrolyzing)</fullName>
            <ecNumber evidence="5">6.3.5.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">asnB</name>
        <name evidence="5" type="ordered locus">NP_2978A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.3.5.4" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323204.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWU7"/>
    <dbReference type="STRING" id="348780.NP_2978A"/>
    <dbReference type="EnsemblBacteria" id="CAI49580">
        <property type="protein sequence ID" value="CAI49580"/>
        <property type="gene ID" value="NP_2978A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702532"/>
    <dbReference type="KEGG" id="nph:NP_2978A"/>
    <dbReference type="eggNOG" id="arCOG00071">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014658_4_0_2"/>
    <dbReference type="OrthoDB" id="8692at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006529">
        <property type="term" value="P:asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050795">
        <property type="entry name" value="Asn_Synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11772">
        <property type="entry name" value="ASPARAGINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11772:SF2">
        <property type="entry name" value="ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Asparagine synthetase" evidence="4">
        <location>
            <begin position="109"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Asparagine synthetase" evidence="4">
        <location>
            <begin position="247"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="362" mass="38110" checksum="14010DB80CF7A0AE" modified="2017-05-10" version="1">MQGTAVEAVRECLETKAPLAGTCGFAGELDGGVVRDVLGRYPAFTEPETPSAWSPDPTDLSEPEPVPAGCIRTADGDRQVWSLPDPEPVADTDRAVSAVADAIDAAIDSVDGAPAVAFSGGIDSSLLAAGLDGPLYVGGFEGSHDITAARSAADLLDRRVRVVEFTHADLERATPTVVEATGRSNPMDVQLALSLYLIAEAVAEDGHDRLALGQGADELFGGYAKVAKAPADPRVDADTVRGARREVVGSLPSQLERDVLAVRAAGIEPVTPLLCDGVVSAALRLPAELLVAGDTRKVALRRAAETVPDGGVPADIRDREKKALQYGSLLAREFDRLARQAGFKRRMDNHLGKYIEDRCQDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWW3</accession>
    <name>A0A1U7EWW3_NATPD</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3014A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWW3"/>
    <dbReference type="STRING" id="348780.NP_3014A"/>
    <dbReference type="EnsemblBacteria" id="CAI49598">
        <property type="protein sequence ID" value="CAI49598"/>
        <property type="gene ID" value="NP_3014A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701628"/>
    <dbReference type="KEGG" id="nph:NP_3014A"/>
    <dbReference type="eggNOG" id="arCOG06445">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1891489_0_0_2"/>
    <dbReference type="OrthoDB" id="198243at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="129" mass="14015" checksum="DE87005F6A846B72" modified="2017-05-10" version="1">MYSLRLDCYRCGTEYGYVGAMPHPGQCPACDSPCVPPAGTLTVTDSLRWESANGLAKVWIRTLDERDRPFEFEIAANGSRGKLAGLKIDGIKIDPNAATALERLPEAVADEIDELGITELDTATREVSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWW5</accession>
    <name>A0A1U7EWW5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">SufB domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">sufB3</name>
        <name evidence="5" type="ordered locus">NP_3016A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the iron-sulfur cluster assembly SufBD family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWW5"/>
    <dbReference type="STRING" id="348780.NP_3016A"/>
    <dbReference type="EnsemblBacteria" id="CAI49599">
        <property type="protein sequence ID" value="CAI49599"/>
        <property type="gene ID" value="NP_3016A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703221"/>
    <dbReference type="KEGG" id="nph:NP_3016A"/>
    <dbReference type="eggNOG" id="arCOG01715">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026231_0_1_2"/>
    <dbReference type="OrthoDB" id="372168at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055346">
        <property type="entry name" value="Fe-S_cluster_assembly_SufBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010231">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000825">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037284">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045595">
        <property type="entry name" value="SufBD_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01980">
        <property type="entry name" value="sufB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30508">
        <property type="entry name" value="FES CLUSTER ASSEMBLY PROTEIN SUF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30508:SF1">
        <property type="entry name" value="UPF0051 PROTEIN ABCI8, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01458">
        <property type="entry name" value="SUFBD_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19295">
        <property type="entry name" value="SufBD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101960">
        <property type="entry name" value="Stabilizer of iron transporter SufD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUF system FeS cluster assembly SufBD N-terminal" evidence="4">
        <location>
            <begin position="139"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="SUF system FeS cluster assembly SufBD core" evidence="3">
        <location>
            <begin position="213"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00043967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF19295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="476" mass="53280" checksum="27AECA36EFDAB03C" modified="2017-05-10" version="1">MSSEEDHLKDTDTEARFEFKKEESSAFKTEKGLTEETIRVISEDKDEPEWMLERRLRALEQFQQMPMPTDWPGQPDLSEVDIDEIVPYIRPDIEARGGAEEWEDLPEEIQDTFDKLGIPEAEKNALSGVGAQYESEVVYQNMQERWEEKGVIFCNMDKAVQEHPELVREYFMTKCVPPSDNKFAALHGAIWSGGSFVYIPEGVTVEMPVQAYFRMNSEGMGQFEHTLIIAEENSEVHYIEGCSAPKYSAFNLHSGGVEVFVKEGAHVQYSTVQNWSKNTYNLNTKRAIAERDATMEWVSGSMGSKATMLYPSTILKGPGATDNHITIAFAGEGQNIDTGAKVYHNAPNTKSTVESKSVAKDGGRTNYRGLVHIADGAENSSTAVECDALMFDNESTSDTMPYMEIEESKVDVAHEATVGKIGDEDVFYLQSRGLDDDDAKQMIVSGFIEPITEELPIEYAVELNRLIELEMEGSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWZ5</accession>
    <name>A0A1U7EWZ5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3088A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323257.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWZ5"/>
    <dbReference type="EnsemblBacteria" id="CAI49635">
        <property type="protein sequence ID" value="CAI49635"/>
        <property type="gene ID" value="NP_3088A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701649"/>
    <dbReference type="KEGG" id="nph:NP_3088A"/>
    <dbReference type="HOGENOM" id="CLU_2730524_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="71" mass="7283" checksum="475088E861549664" modified="2017-05-10" version="1">MEWTKPLSAALAVLIALLAVGSAVQLGQTTFTTGPDVIESIATIVFVGVVVVAAIAVGARGGQWLDNPGYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX31</accession>
    <name>A0A1U7EX31_NATPD</name>
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        <submittedName>
            <fullName evidence="10">RND superfamily permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">NP_3130A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family. MmpL subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="CAI49656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX31"/>
    <dbReference type="STRING" id="348780.NP_3130A"/>
    <dbReference type="EnsemblBacteria" id="CAI49656">
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        <property type="gene ID" value="NP_3130A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701662"/>
    <dbReference type="KEGG" id="nph:NP_3130A"/>
    <dbReference type="eggNOG" id="arCOG02174">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003088_0_0_2"/>
    <dbReference type="OrthoDB" id="42357at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004869">
        <property type="entry name" value="MMPL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050545">
        <property type="entry name" value="Mycobact_MmpL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33406">
        <property type="entry name" value="MEMBRANE PROTEIN MJ1562-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33406:SF6">
        <property type="entry name" value="MEMBRANE PROTEIN YDGH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03176">
        <property type="entry name" value="MMPL"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="SSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="266"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="291"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="320"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="360"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="392"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="681"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="708"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="782"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="811"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="9">
        <location>
            <begin position="294"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="9">
        <location>
            <begin position="678"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="140"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="141"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI49656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="855" mass="92110" checksum="D07923813DB1CD5A" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX45</accession>
    <name>A0A1U7EX45_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transport protein (Probable substrate zinc/cadmium/cobalt)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">tp38</name>
        <name evidence="9" type="ordered locus">NP_3196A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323311.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX45"/>
    <dbReference type="STRING" id="348780.NP_3196A"/>
    <dbReference type="EnsemblBacteria" id="CAI49689">
        <property type="protein sequence ID" value="CAI49689"/>
        <property type="gene ID" value="NP_3196A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703314"/>
    <dbReference type="KEGG" id="nph:NP_3196A"/>
    <dbReference type="eggNOG" id="arCOG01474">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013430_3_6_2"/>
    <dbReference type="OrthoDB" id="8907at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1350">
        <property type="entry name" value="Cation efflux protein, cytoplasmic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027470">
        <property type="entry name" value="Cation_efflux_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050291">
        <property type="entry name" value="CDF_Transporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43840">
        <property type="entry name" value="MITOCHONDRIAL METAL TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43840:SF15">
        <property type="entry name" value="MITOCHONDRIAL METAL TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16916">
        <property type="entry name" value="ZT_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160240">
        <property type="entry name" value="Cation efflux protein cytoplasmic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="122"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="7">
        <location>
            <begin position="25"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein cytoplasmic" evidence="8">
        <location>
            <begin position="221"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI49689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="323" mass="34456" checksum="1B98C32506F8CAD2" modified="2017-05-10" version="1">MRTTAAEEAGLETETARRRFSRASLVNVAGNAVKILVEGAVGLAFGSVALLADAAHSVADLVASAVVLVWGRSVYTDPDENHPHGHQRVEPLAALFVGSLIVLLGLNLFYESATGLVAGPEVQFHPLLVGALLFAMADMYLLYWYTTHINESVGSSALEALAIDCRNDIYTTIAALCGVIGVFFGYPLFDAVAGGLVSVLVVYQGFEISRENVSYLVGASPSDQQRQRVVETLTDHPAVHGAHDVAVFYDGTDIEVEAHVEVDGELTLVEAHDIETELVSALRSLESVGDVHLHLDPAGIGEWKDAAVNEQRGPETQTGSPKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX72</accession>
    <name>A0A1U7EX72_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">CRISPR-associated exonuclease Cas4</fullName>
            <ecNumber evidence="3 13">3.1.12.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">cas4</name>
        <name evidence="15" type="ordered locus">NP_3272A</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="13">Mg(2+) or Mn(2+) required for ssDNA cleavage activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>iron-sulfur cluster</name>
            <dbReference type="ChEBI" id="CHEBI:30408"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the CRISPR-associated exonuclease Cas4 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.12.1" evidence="3 13"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX72"/>
    <dbReference type="STRING" id="348780.NP_3272A"/>
    <dbReference type="EnsemblBacteria" id="CAI49727">
        <property type="protein sequence ID" value="CAI49727"/>
        <property type="gene ID" value="NP_3272A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3272A"/>
    <dbReference type="eggNOG" id="arCOG00786">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102055_1_0_2"/>
    <dbReference type="OrthoDB" id="26676at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051827">
        <property type="entry name" value="Cas4_exonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013343">
        <property type="entry name" value="CRISPR-assoc_prot_Cas4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022765">
        <property type="entry name" value="Dna2/Cas4_DUF83"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00372">
        <property type="entry name" value="cas4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36531">
        <property type="entry name" value="CRISPR-ASSOCIATED EXONUCLEASE CAS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36531:SF6">
        <property type="entry name" value="DNA REPLICATION ATP-DEPENDENT HELICASE_NUCLEASE DNA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01930">
        <property type="entry name" value="Cas_Cas4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="8 13 15" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="7 13 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 13" id="KW-0408">Iron</keyword>
    <keyword evidence="10 13" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="12 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 13" id="KW-0540">Nuclease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF83" evidence="14">
        <location>
            <begin position="17"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAI49727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="205" mass="23257" checksum="E9290ACAC151BAF4" modified="2017-05-10" version="1">MTPANSVTDHDECVHVSALNEYLYCPRRFYYQRYHDEMGTPYELVDGRSKHENAAQRGDWINERYLSDSSLGLHGKIDIIESKDGVLTPIERKRAESGGYYTNDEVQLAGYCMLLSNAIGEPVNVGYIYLYSTDQRHSIRITEDHRQAVTKIVDQIQSMSATNIPPLTDNPDKCDACSAREYCMPAETAKLEPEKAHGTGWEDEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX97</accession>
    <name>A0A1U7EX97_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type transport system permease protein (Probable substrate branched-chain amino acids)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">livM4</name>
        <name evidence="7" type="synonym">abc08p2</name>
        <name evidence="7" type="ordered locus">NP_3428A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323425.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX97"/>
    <dbReference type="STRING" id="348780.NP_3428A"/>
    <dbReference type="EnsemblBacteria" id="CAI49805">
        <property type="protein sequence ID" value="CAI49805"/>
        <property type="gene ID" value="NP_3428A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702390"/>
    <dbReference type="KEGG" id="nph:NP_3428A"/>
    <dbReference type="eggNOG" id="arCOG01275">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031365_0_1_2"/>
    <dbReference type="OrthoDB" id="30958at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06581">
        <property type="entry name" value="TM_PBP1_LivM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043428">
        <property type="entry name" value="LivM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30482:SF17">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30482">
        <property type="entry name" value="HIGH-AFFINITY BRANCHED-CHAIN AMINO ACID TRANSPORT SYSTEM PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="16"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="119"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="261"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="296"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="334" mass="36197" checksum="A5C9D0A4C4CE24FF" modified="2017-05-10" version="1">MSRLGRIRNALGDKRVLGGIGLFVLLALIPATGRGFESFLVIWMMCFAIAALGFNVLLGYTGLLSFGHAAFFGSAAYAMAIGMDRLGIESLIVLLVFAVAVAGLLGLAMALLTLRSNEIYFALLTLALAQLVYVVGVRDIYGLSGGTDGMSVGTPDFLGLGLNELAFNTYMLGFYYYIVLLFLAGTILLMWAMLRSPFGLTLKMIRENPERAMMSGVPVERYRTYSMTLSAMITGLGGALYAVQLGRITPDQLYWVQSGEIVFMALLGGMGTFFGPIVGAAAFIGLQELTLRFTEYWHFVMGLILLIVVVTLREDGIWGGIKQASERFRGGDDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXH9</accession>
    <name>A0A1U7EXH9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">ABC-type transport system permease protein (Probable substrate dipeptide/oligopeptide)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">dppB2</name>
        <name evidence="12" type="synonym">abc02p1</name>
        <name evidence="12" type="ordered locus">NP_3580A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the binding-protein-dependent transport system permease family. OppBC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323501.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXH9"/>
    <dbReference type="STRING" id="348780.NP_3580A"/>
    <dbReference type="EnsemblBacteria" id="CAI49881">
        <property type="protein sequence ID" value="CAI49881"/>
        <property type="gene ID" value="NP_3580A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702368"/>
    <dbReference type="KEGG" id="nph:NP_3580A"/>
    <dbReference type="eggNOG" id="arCOG00751">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036879_0_2_2"/>
    <dbReference type="OrthoDB" id="44105at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015099">
        <property type="term" value="F:nickel cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045621">
        <property type="entry name" value="BPD_transp_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050045">
        <property type="entry name" value="Opp2B"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045470">
        <property type="entry name" value="Opp2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43163">
        <property type="entry name" value="DIPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN DPPB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43163:SF6">
        <property type="entry name" value="DIPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN DPPB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19300">
        <property type="entry name" value="BPD_transp_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0533">Nickel</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="104"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="139"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="232"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="278"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="100"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI49881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="315" mass="33541" checksum="12869F883101BDFE" modified="2017-05-10" version="1">MTGRYAARRLGTALVTLFFISVLTYALIFIAPGDPAEASLRAQLGEQPTQEQIEIFRVEHGLDEPFHVQYASWVADLLQGDLGESYYHERSVAELIGDRLPTTLALAAGGMGVALLVSVPTGVASAVRPNGYIDHASQLFALVGVAMPNFWLGYLLIFLFAITLGLVPVSGTGGLRSLMLPSITLGTGLAAVQTRLLRSALLDVLDHEFVDASRARGLGERVVVYKHALRNALVPVITVVGLQLGMAINGAVIVEVVFQRPGLGALLVEAVFDRDYPVVQGVTLLAGATFIVVNVATDVVYRRLDPRISIGGAGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXK3</accession>
    <name>A0A1U7EXK3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Mechanosensitive channel protein MscS</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">mscS4</name>
        <name evidence="10" type="ordered locus">NP_3628A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323525.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXK3"/>
    <dbReference type="STRING" id="348780.NP_3628A"/>
    <dbReference type="EnsemblBacteria" id="CAI49905">
        <property type="protein sequence ID" value="CAI49905"/>
        <property type="gene ID" value="NP_3628A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702941"/>
    <dbReference type="KEGG" id="nph:NP_3628A"/>
    <dbReference type="eggNOG" id="arCOG01568">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062814_0_0_2"/>
    <dbReference type="OrthoDB" id="11475at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049142">
        <property type="entry name" value="MS_channel_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045275">
        <property type="entry name" value="MscS_archaea/bacteria_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_beta-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011066">
        <property type="entry name" value="MscS_channel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011014">
        <property type="entry name" value="MscS_channel_TM-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30221">
        <property type="entry name" value="SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30221:SF1">
        <property type="entry name" value="SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21088">
        <property type="entry name" value="MS_channel_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82689">
        <property type="entry name" value="Mechanosensitive channel protein MscS (YggB), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82861">
        <property type="entry name" value="Mechanosensitive channel protein MscS (YggB), transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="94"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel transmembrane helices 2/3" evidence="9">
        <location>
            <begin position="68"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS" evidence="8">
        <location>
            <begin position="110"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI49905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="284" mass="31365" checksum="EA4EF8EEB924A3A0" modified="2017-05-10" version="1">MVGVQPPLLQLAPPDGVEMPEVGVGTVAGAVLILLAAYALARASVFILTKLSDRFPTRRIGIRMATPIIKFGIYIVAVFIILDSLLELTTTQVLAFSGVLGAALGFGLRDLFAGMVGGLVIILERPYRVGDEVEIKDHYGEITDIGLRATRIETPDDDLVSVPNYVFFTDAVANANAGNQEMMVVTEFMISPEADIDRAQAIVEEAVVTSRYVYITDDCPYTVSMRDRPYYYLIRVRAYVNDHRYERALISDITERVFEQFQAEGIPKPRGLDRDLHGEATEDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXN2</accession>
    <name>A0A1U7EXN2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PRC domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3706A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323562.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXN2"/>
    <dbReference type="STRING" id="348780.NP_3706A"/>
    <dbReference type="EnsemblBacteria" id="CAI49944">
        <property type="protein sequence ID" value="CAI49944"/>
        <property type="gene ID" value="NP_3706A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701844"/>
    <dbReference type="KEGG" id="nph:NP_3706A"/>
    <dbReference type="eggNOG" id="arCOG02155">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170070_0_0_2"/>
    <dbReference type="OrthoDB" id="68960at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.240">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38137">
        <property type="entry name" value="PRC-BARREL DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38137:SF1">
        <property type="entry name" value="PRC-BARREL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRC-barrel" evidence="1">
        <location>
            <begin position="10"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="96" mass="10493" checksum="8DD290FC2BC51E1C" modified="2017-05-10" version="1">METESAPQEITSLVGREVYSNNGVFVGEVEDLQLNLDRESVTGLALGELNRELFAGHVPSSDGVIVPYRWVRAVGDIILVNDAVEKIQEPEEPDAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXR5</accession>
    <name>A0A1U7EXR5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Enoyl-CoA hydratase</fullName>
            <ecNumber evidence="3">4.2.1.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">fadA8</name>
        <name evidence="3" type="ordered locus">NP_3830A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.17" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323623.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXR5"/>
    <dbReference type="STRING" id="348780.NP_3830A"/>
    <dbReference type="DNASU" id="3702685"/>
    <dbReference type="EnsemblBacteria" id="CAI50006">
        <property type="protein sequence ID" value="CAI50006"/>
        <property type="gene ID" value="NP_3830A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702685"/>
    <dbReference type="KEGG" id="nph:NP_3830A"/>
    <dbReference type="eggNOG" id="arCOG00239">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_4_2"/>
    <dbReference type="OrthoDB" id="203755at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43802">
        <property type="entry name" value="ENOYL-COA HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43802:SF1">
        <property type="entry name" value="IP11341P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="232" mass="24232" checksum="619D11479412C32B" modified="2017-05-10" version="1">MIHTDDTDGVRVVTLDRPERRNALTADGLDALRTAIEACPEPVAYLRGAGESFCAGADLSTVADAAAEGAAAEFIERGQATADAIENTDTVVVAGIDGPARGGGVELALACDIRVATPTATLGEPGVTFGLFGAWGGTVRLPEVVGLGAALDFSLSGRVLDAEEAKQIGLVSRVCTDPRSVAESVADNDPLALEHLKRRLRDRAEKSAQQRAEREAFETLAAAHSDRLQDLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXR7</accession>
    <name>A0A1U7EXR7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF3054 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3794A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323605.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXR7"/>
    <dbReference type="STRING" id="348780.NP_3794A"/>
    <dbReference type="EnsemblBacteria" id="CAI49988">
        <property type="protein sequence ID" value="CAI49988"/>
        <property type="gene ID" value="NP_3794A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701866"/>
    <dbReference type="KEGG" id="nph:NP_3794A"/>
    <dbReference type="eggNOG" id="arCOG03922">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143267_0_0_2"/>
    <dbReference type="OrthoDB" id="177006at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021414">
        <property type="entry name" value="DUF3054"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11255">
        <property type="entry name" value="DUF3054"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="137" mass="14686" checksum="8B84A4AD948DA56F" modified="2017-05-10" version="1">MSEQSFLEQRLDASTWPIAVGDIVVLLAFIFLGTLSHWPLDAMLADPTIYLAAAGPFLLGWVICAPLIGAYSPGGGSAPNSSIPLAIRSWVPAAIVGFVVRYVALPQRGVEPVFIGIMLVGGAVVISAWRFLYFKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXS1</accession>
    <name>A0A1U7EXS1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cobalamin import system permease protein BtuC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">btuC</name>
        <name evidence="12" type="synonym">abc16p:fhuB</name>
        <name evidence="12" type="ordered locus">NP_3816A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Required for corrinoid utilization. Probably part of the ABC transporter complex BtuCDF involved in cobalamin (vitamin B12) import. Probably involved in the translocation of the substrate across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">The complex is composed of two ATP-binding proteins (BtuD), two transmembrane proteins (BtuC) and a solute-binding protein (BtuF).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323616.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXS1"/>
    <dbReference type="STRING" id="348780.NP_3816A"/>
    <dbReference type="EnsemblBacteria" id="CAI49999">
        <property type="protein sequence ID" value="CAI49999"/>
        <property type="gene ID" value="NP_3816A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702418"/>
    <dbReference type="KEGG" id="nph:NP_3816A"/>
    <dbReference type="eggNOG" id="arCOG01007">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013016_0_1_2"/>
    <dbReference type="OrthoDB" id="57034at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06550">
        <property type="entry name" value="TM_ABC_iron-siderophores_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3470.10:FF:000001">
        <property type="entry name" value="Vitamin B12 ABC transporter permease BtuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="entry name" value="ABC transporter involved in vitamin B12 uptake, BtuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000522">
        <property type="entry name" value="ABC_transptr_permease_BtuC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007081">
        <property type="entry name" value="PRK09535.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472:SF25">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE PROTEIN MJ0876-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472">
        <property type="entry name" value="FERRIC ENTEROBACTIN TRANSPORT SYSTEM PERMEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01032">
        <property type="entry name" value="FecCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="ABC transporter involved in vitamin B12 uptake, BtuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="138"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="255"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="322"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00071366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI49999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="348" mass="36488" checksum="7506275978F63002" modified="2017-05-10" version="1">MVLRRTLSWSAGLTGLLVVTVLTSTALGYAEIGPLEVLYVVLNAIRLPTLADGGVAWVQPFGFPVDESTRLIVTQIRLPRILLGAIVGFALALAGAVMQGFFRNPMADPSIIGVSSGAAVGAVAFIVLPLSVPFGRTAAAFAGALVAAFAVYLIATEDGRTPVATLLLAGVALQTFLGAVISYLLLHAGDNLETAVYWLMGHLQDSTWGRVRLALPLVVFFFVVLLAYARDLNVLLLGEEDAHTLGIEVERTKRILLAASSLVTAAAVAVSGVIGFVGLIVPHMMRLLVGPDHRILLPTSALAGAVFLVATDTVARSGPGQLPVGIVTAALGAPFFLYLLRKREVHAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY02</accession>
    <name>A0A1U7EY02_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14 15">Shikimate kinase</fullName>
            <shortName evidence="14">SK</shortName>
            <ecNumber evidence="4 14 15">2.7.1.71</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14 17" type="primary">aroK</name>
        <name evidence="17" type="ordered locus">NP_3982A</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>shikimate + ATP = 3-phosphoshikimate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13121"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.71"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the GHMP kinase family. Archaeal shikimate kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.71" evidence="4 14 15"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50082.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939643.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY02"/>
    <dbReference type="STRING" id="348780.NP_3982A"/>
    <dbReference type="EnsemblBacteria" id="CAI50082">
        <property type="protein sequence ID" value="CAI50082"/>
        <property type="gene ID" value="NP_3982A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702530"/>
    <dbReference type="KEGG" id="nph:NP_3982A"/>
    <dbReference type="eggNOG" id="arCOG01025">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073768_0_0_2"/>
    <dbReference type="OrthoDB" id="9602at2157"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00088"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004765">
        <property type="term" value="F:shikimate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00370">
        <property type="entry name" value="Shik_kinase_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010189">
        <property type="entry name" value="SK_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01920">
        <property type="entry name" value="Shik_kin_archae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861">
        <property type="entry name" value="HOMOSERINE/4-DIPHOSPHOCYTIDYL-2-C-METHYL-D-ERYTHRITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861:SF3">
        <property type="entry name" value="SHIKIMATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005758">
        <property type="entry name" value="Shikimt_kin_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="12 14" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="11 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 14 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14 17" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="16">
        <location>
            <begin position="57"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="84"/>
            <end position="94"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="NCBIfam" id="TIGR01920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAI50082.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="280" mass="29146" checksum="D41C48EF0E4D8765" modified="2017-05-10" version="1">MEGRAAAPAAGTVLNALANGYGSAFAIDAYTEATVTLDDTGSVTGEIADAPDADTRLIERCVEAVVERFGDGQGGHVRTESDVPMASGLKSSSAAANATVLATLAALDVDLDREAACRIGVDAARDVGVTVTGAFDDASASMLGGVTVTDNTSDELLQRDEPDWDVLVYTPDERAFSADADADRCERIAPMADVVYELARDGDYERAMCVNGFAFCAALGYPTDPLVEALPAAAASLSGTGPSYTAVGDRETLESLETTWSNREGHTWLTTTQQTGATRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY09</accession>
    <name>A0A1U7EY09_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Beta-lactamase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">NP_3970A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-beta-lactamase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323692.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY09"/>
    <dbReference type="STRING" id="348780.NP_3970A"/>
    <dbReference type="EnsemblBacteria" id="CAI50076">
        <property type="protein sequence ID" value="CAI50076"/>
        <property type="gene ID" value="NP_3970A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701927"/>
    <dbReference type="KEGG" id="nph:NP_3970A"/>
    <dbReference type="eggNOG" id="arCOG00511">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030571_3_2_2"/>
    <dbReference type="OrthoDB" id="7773at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07729">
        <property type="entry name" value="AHL_lactonase_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051013">
        <property type="entry name" value="MBL_superfamily_lactonases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42978:SF2">
        <property type="entry name" value="102 KBASES UNSTABLE REGION: FROM 1 TO 119443"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42978">
        <property type="entry name" value="QUORUM-QUENCHING LACTONASE YTNP-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="6">
        <location>
            <begin position="43"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI50076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="267" mass="30121" checksum="97D63DBE93E5BAC4" modified="2017-05-10" version="1">MVDADIHLLDRGTIRTDSNHIIEGHITASADNPHPDVALVDGPVYNLLIDHPEGTILWDTGSHPEAGDGHWPDELYAAFEHADADERDLDTALGEVGYTVDDIDYVVQSHLHLDHAGGLYRFADTDVPVFVHEAELKYAYYSAKTDAGSTAYLADDFDHNLNWEIVHRDGETHFEDIEFLHLPGHTPGLLGLSIDLDDEGRVLIAGDEFYTRTNFEDEQPLGAGLLWSKRDWYDSLRKIQERQRRHDVRVFCGHDPVDVATLRDEFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY31</accession>
    <name>A0A1U7EY31_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DICT domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4048A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323731.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY31"/>
    <dbReference type="STRING" id="348780.NP_4048A"/>
    <dbReference type="EnsemblBacteria" id="CAI50115">
        <property type="protein sequence ID" value="CAI50115"/>
        <property type="gene ID" value="NP_4048A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701951"/>
    <dbReference type="KEGG" id="nph:NP_4048A"/>
    <dbReference type="eggNOG" id="arCOG02909">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069298_0_0_2"/>
    <dbReference type="OrthoDB" id="198447at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019278">
        <property type="entry name" value="DICT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016954">
        <property type="entry name" value="Uncharacterised_Vng0742h"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10069">
        <property type="entry name" value="DICT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF030471">
        <property type="entry name" value="STR_Vng0742h_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DICT" evidence="1">
        <location>
            <begin position="92"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="242" mass="27101" checksum="0930930A0197CD65" modified="2017-05-10" version="1">MSLTELIAGVEDHEKRLTVFNADSDAVAALSEQFHDRNVDVVGEHTPSGRPKTFAVLSSDGSFVTAADVDNILEPTTTEETEPGFDGEAYQPILDHLDETMFTSYDIGQMVAASREIEDRAWRVGKGTLHSGFQKLSILQDQMDIYEQLAEKKHLEVHAYAVPDADVPDHDSDLTIHVERSDEIERSWFVAYDGAGVDVNKCALLAEEREPRSFYGFWTYDPSTVDWIIDHLDSTYGLVESQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY48</accession>
    <name>A0A1U7EY48_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PAC2 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4074A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323744.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY48"/>
    <dbReference type="STRING" id="348780.NP_4074A"/>
    <dbReference type="EnsemblBacteria" id="CAI50128">
        <property type="protein sequence ID" value="CAI50128"/>
        <property type="gene ID" value="NP_4074A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701960"/>
    <dbReference type="KEGG" id="nph:NP_4074A"/>
    <dbReference type="eggNOG" id="arCOG00347">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075000_0_0_2"/>
    <dbReference type="OrthoDB" id="35908at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10900">
        <property type="entry name" value="PAC-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019151">
        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35610:SF8">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35610">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09754">
        <property type="entry name" value="PAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159659">
        <property type="entry name" value="Cgl1923-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="246" mass="26163" checksum="B91F651490A50F23" modified="2017-05-10" version="1">MASVQIHNDDIQLESPVFVEGLPGAGLVGKIAADHLIETFDMDYYAAFHCEGLPQAAVYEAEKSVVRPPVRLYADSDRDIVVLQSDVPVSPTQASEFAGCVTGWIQSTDALPLYLSGLAEEKEGVPELYGVATGDAEPVLDDAGIVPPRGGGMVTGPTGALLHRAAEEAIDAVGLIVQTNPQFPDPEAARVILEHGVEPIAGIDVETDELVDRADEIQQAREQLAKRVQEAANEESTAAQPIRGFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY60</accession>
    <name>A0A1U7EY60_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TrmB family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4124A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323769.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY60"/>
    <dbReference type="STRING" id="348780.NP_4124A"/>
    <dbReference type="EnsemblBacteria" id="CAI50153">
        <property type="protein sequence ID" value="CAI50153"/>
        <property type="gene ID" value="NP_4124A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701981"/>
    <dbReference type="KEGG" id="nph:NP_4124A"/>
    <dbReference type="eggNOG" id="arCOG02242">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140786_0_0_2"/>
    <dbReference type="OrthoDB" id="9141at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator TrmB N-terminal" evidence="1">
        <location>
            <begin position="19"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="134" mass="15483" checksum="0D6F93912C89B825" modified="2017-05-10" version="1">MANSMSEYLKQDMECEGLLECIHGLKELDKEIFQLLTETAEPMTVDEIADAVDRERSTAYRSVQRLVQSGFVQKEQVNYDQGGYYHVFRPTDPDEIADDMQRMLNDWYAKMGNLIGEFREKYDDPAEQAAVAEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY65</accession>
    <name>A0A1U7EY65_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DMT superfamily transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_4156A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323785.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY65"/>
    <dbReference type="STRING" id="348780.NP_4156A"/>
    <dbReference type="EnsemblBacteria" id="CAI50169">
        <property type="protein sequence ID" value="CAI50169"/>
        <property type="gene ID" value="NP_4156A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701988"/>
    <dbReference type="KEGG" id="nph:NP_4156A"/>
    <dbReference type="eggNOG" id="arCOG03427">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151702_0_0_2"/>
    <dbReference type="OrthoDB" id="156473at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="3"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="139" mass="14543" checksum="067A84041281D487" modified="2017-05-10" version="1">MQYIWYALVALAAYALVAPLTKLATQELPADTVAMVTNGMLVVAAFGLVVATDRPILSALTHEHAPYMYAAGVCLAVGIIAYYRALAAGPVSVVVPIFGLFLVASSVIGILFLDEALTLRKATGIVLAVVAVFLTTFEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY81</accession>
    <name>A0A1U7EY81_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Probable oxidoreductase (Short-chain dehydrogenase family)</fullName>
            <ecNumber evidence="3">1.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_4166A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323790.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY81"/>
    <dbReference type="STRING" id="348780.NP_4166A"/>
    <dbReference type="EnsemblBacteria" id="CAI50174">
        <property type="protein sequence ID" value="CAI50174"/>
        <property type="gene ID" value="NP_4166A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701991"/>
    <dbReference type="KEGG" id="nph:NP_4166A"/>
    <dbReference type="eggNOG" id="arCOG01259">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_1_2"/>
    <dbReference type="OrthoDB" id="281764at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760:SF133">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="251" mass="26072" checksum="C9DC9A62EEA1A95E" modified="2017-05-10" version="1">MELDLSGKTAVVTGGSRGIGRAISLGFAAAGADVVPLSRTESDVEAVVEDIESHGVESRVETLDVADSDAVEACFERIDDALGIDVVVNNAGINPDAALGTPESVPDEGFDSVLDVNLGGAFACARAAEPTLRDNGGSLINVASVGGLVGLPRQHPYVASKHGLVGLTKSLALDWAPDVRVNCLAPGYVATDLTDDLQENEDLRRSIERRTPLDRFAEPEEIAGPAVFLASDLASYATGEVFAVDGGWTAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYB2</accession>
    <name>A0A1U7EYB2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Assimilatory nitrate reductase</fullName>
            <ecNumber evidence="7">1.7.7.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">nasA1</name>
        <name evidence="7" type="synonym">narB1</name>
        <name evidence="7" type="ordered locus">NP_4226A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAI50204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323820.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYB2"/>
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    <dbReference type="EnsemblBacteria" id="CAI50204">
        <property type="protein sequence ID" value="CAI50204"/>
        <property type="gene ID" value="NP_4226A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702968"/>
    <dbReference type="KEGG" id="nph:NP_4226A"/>
    <dbReference type="eggNOG" id="arCOG01491">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_13_4_2"/>
    <dbReference type="OrthoDB" id="23466at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047889">
        <property type="term" value="F:ferredoxin-nitrate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:molybdopterin cofactor binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ADC-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Dimethylsulfoxide Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
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        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
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        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
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        <property type="entry name" value="Nitr_red_NasA"/>
    </dbReference>
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        <property type="entry name" value="Prok_molybdopt-oxidoreductase"/>
    </dbReference>
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        <property type="entry name" value="Nitr_red_NasA_Halo"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NADPH-FE(3+) OXIDOREDUCTASE SUBUNIT ALPHA"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR43105">
        <property type="entry name" value="RESPIRATORY NITRATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="6">
        <location>
            <begin position="2"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI50204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="696" mass="73685" checksum="1F8BE4EBE332E2E9" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYE0</accession>
    <name>A0A1U7EYE0_NATPD</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4260A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323837.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYE0"/>
    <dbReference type="STRING" id="348780.NP_4260A"/>
    <dbReference type="EnsemblBacteria" id="CAI50221">
        <property type="protein sequence ID" value="CAI50221"/>
        <property type="gene ID" value="NP_4260A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702013"/>
    <dbReference type="KEGG" id="nph:NP_4260A"/>
    <dbReference type="eggNOG" id="arCOG02804">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099321_0_0_2"/>
    <dbReference type="OrthoDB" id="304916at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048925">
        <property type="entry name" value="RdfA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21811">
        <property type="entry name" value="RdfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="213" mass="24250" checksum="9E3861EE2B3D7F33" modified="2017-05-10" version="1">MSEPEKKSSEAERESSRGRRSKVARLLDDYDMAGVGAELERQWTADEDRMSLRDLASYFNRQLLQQRLEAANVRPVDGEVANIYRILTDDDDVAEADKTRVRRRLERDGIDVETLENDFVTYQAIRTYLKSYRGAEYSPDETDPIEREITNLQKLRGRVDAATTGKLEQLGRSGDLDIGAFRTLVDIRVVCEDCGTQYEAVELLERGGCDCAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYH9</accession>
    <name>A0A1U7EYH9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Propionyl-CoA carboxylase carboxyltransferase component</fullName>
            <ecNumber evidence="6">6.4.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">pccB2</name>
        <name evidence="6" type="ordered locus">NP_4364A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AccD/PCCB family.</text>
    </comment>
    <dbReference type="EC" id="6.4.1.3" evidence="6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323889.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYH9"/>
    <dbReference type="STRING" id="348780.NP_4364A"/>
    <dbReference type="EnsemblBacteria" id="CAI50273">
        <property type="protein sequence ID" value="CAI50273"/>
        <property type="gene ID" value="NP_4364A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702470"/>
    <dbReference type="KEGG" id="nph:NP_4364A"/>
    <dbReference type="eggNOG" id="arCOG02705">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018822_6_2_2"/>
    <dbReference type="OrthoDB" id="30579at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009317">
        <property type="term" value="C:acetyl-CoA carboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003989">
        <property type="term" value="F:acetyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004658">
        <property type="term" value="F:propionyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000017">
        <property type="entry name" value="Propionyl-CoA carboxylase subunit beta 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000016">
        <property type="entry name" value="Propionyl-CoA carboxylase, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051047">
        <property type="entry name" value="AccD/PCCB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000438">
        <property type="entry name" value="Acetyl_CoA_COase_Trfase_b_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43842">
        <property type="entry name" value="PROPIONYL-COA CARBOXYLASE BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43842:SF2">
        <property type="entry name" value="PROPIONYL-COA CARBOXYLASE BETA CHAIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01070">
        <property type="entry name" value="ACCCTRFRASEB"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="4">
        <location>
            <begin position="3"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="5">
        <location>
            <begin position="263"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="442"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="10"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI50273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="516" mass="57144" checksum="A416AD8405F055E4" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYI8</accession>
    <name>A0A1U7EYI8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CopD domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_4396A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323905.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYI8"/>
    <dbReference type="STRING" id="348780.NP_4396A"/>
    <dbReference type="EnsemblBacteria" id="CAI50289">
        <property type="protein sequence ID" value="CAI50289"/>
        <property type="gene ID" value="NP_4396A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702049"/>
    <dbReference type="KEGG" id="nph:NP_4396A"/>
    <dbReference type="eggNOG" id="arCOG06302">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1801634_0_0_2"/>
    <dbReference type="OrthoDB" id="205725at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008457">
        <property type="entry name" value="Cu-R_CopD_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05425">
        <property type="entry name" value="CopD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Copper resistance protein D" evidence="2">
        <location>
            <begin position="41"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="142" mass="14713" checksum="D7DE00DDDCF157F0" modified="2017-05-10" version="1">MVLSTYAAVRTLHVLAVSLLVGGSAVLWLGLRVGTARLPVRLLSWFEAVFWGGLGIVVFTGLGNLVAFGIPPVDSHRGIVLTAKFVAVLGLAVFALVRAAAVIELQRAGNGAVGGRRLRLLYAATAWLSTAAVLLAGVLARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYK4</accession>
    <name>A0A1U7EYK4_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7 8">Threonine synthase</fullName>
            <ecNumber evidence="7 8">4.2.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">thrC2</name>
        <name evidence="12" type="ordered locus">NP_4464A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the gamma-elimination of phosphate from L-phosphohomoserine and the beta-addition of water to produce L-threonine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>O-phospho-L-homoserine + H2O = L-threonine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10840"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57590"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="EC" id="4.2.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8 9">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the threonine synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.1" evidence="7 8"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323938.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYK4"/>
    <dbReference type="STRING" id="348780.NP_4464A"/>
    <dbReference type="EnsemblBacteria" id="CAI50323">
        <property type="protein sequence ID" value="CAI50323"/>
        <property type="gene ID" value="NP_4464A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703254"/>
    <dbReference type="KEGG" id="nph:NP_4464A"/>
    <dbReference type="eggNOG" id="arCOG01434">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028142_4_1_2"/>
    <dbReference type="OrthoDB" id="6371at2157"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00065"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:threonine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004795">
        <property type="term" value="F:threonine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006565">
        <property type="term" value="P:L-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01563">
        <property type="entry name" value="Thr-synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050147">
        <property type="entry name" value="Ser/Thr_Dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004450">
        <property type="entry name" value="Thr_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026260">
        <property type="entry name" value="Thr_Synthase_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00260">
        <property type="entry name" value="thrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078:SF6">
        <property type="entry name" value="L-THREONINE DEHYDRATASE CATABOLIC TDCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038945">
        <property type="entry name" value="Thr_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 8 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 8 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="11">
        <location>
            <begin position="75"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="374"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="10">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="NCBIfam" id="TIGR00260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR038945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR038945-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR038945-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI50323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="409" mass="43243" checksum="27CDF37C9C9C1920" modified="2017-05-10" version="1">MDHVDSLECVRCGAEYDPNQLRYTCPNHPGVTGILEVTYDYDIIVDRFDEPLDGAIDSQWKYRAFLPVEAEADPVTLGEGGTPLLSAPQLGAELDVDLHIKDDGRNPTGVLKDRASSVSVTKAAEFDADVVTCASTGNAAASLAGFAARGDFDCRIFVPEDAPEEKLAQPLVYGADVLGVQGSYDEAYDLSMEATDRFGWYNRNAAVNPFQVEGKRTVGHELAEETRGAVPDWLVFAMGDGCTIAGGWKGFREFADLGFVEETPRMLGVQADGASAIHDAFHEFDDVDAVADTVADSISVGRPRNTVKACSALEASGGTAVLVDDTDILEAESLLGRTEGIYAEPAGATPVAGLRNARENGIIEPGESVVVVVTGTGLKDAASAMRAGGNVDTIAPAIDDVADRYSADL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYL1</accession>
    <name>A0A1U7EYL1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">UPF0324 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">NP_4466A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323939.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYL1"/>
    <dbReference type="STRING" id="348780.NP_4466A"/>
    <dbReference type="EnsemblBacteria" id="CAI50324">
        <property type="protein sequence ID" value="CAI50324"/>
        <property type="gene ID" value="NP_4466A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702070"/>
    <dbReference type="KEGG" id="nph:NP_4466A"/>
    <dbReference type="eggNOG" id="arCOG03873">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033541_1_0_2"/>
    <dbReference type="OrthoDB" id="26684at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018383">
        <property type="entry name" value="UPF0324_pro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30106">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YEIH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30106:SF1">
        <property type="entry name" value="UPF0324 MEMBRANE PROTEIN FN0533"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03601">
        <property type="entry name" value="Cons_hypoth698"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="177"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="213"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="251"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="274"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="304"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI50324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="329" mass="33632" checksum="A430D7622F878480" modified="2017-05-10" version="1">MVAGRRKVVPGLVLLCLGALLARSLSVLIGINELLLAIALGAVLVNTVGIPERFRHGTATYNIWLAAGIVLLGSSLTVGMIIEVGATVLLLLVAAVTLTLVTVEVLARNVAGVTDRFGSLLAAGAGICGVSAVAAVGGAVRAKEMHIAYAAGVVLLVDAVTIVVYPIVGAVLDLPDVVFGVWAGVSMLSTGPVVAVGFAHSEVAGQWATITKLARNALIGVVALGYASYYARRGAGGSTSVRMLWENFPKFVLGFLVLVGLASLGAFSPAQQDMISTVVDWLFLLAFVGLGTQIRTAELRATGITPALIVFAAVLLVSVLSLAAALVVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYL6</accession>
    <name>A0A1U7EYL6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein P1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 6" type="primary">rpl12</name>
        <name evidence="6" type="ordered locus">NP_4448A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit. Homodimer, it forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Forms a heptameric L10(L12)2(L12)2(L12)2 complex, where L10 forms an elongated spine to which the L12 dimers bind in a sequential fashion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323930.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYL6"/>
    <dbReference type="STRING" id="348780.NP_4448A"/>
    <dbReference type="EnsemblBacteria" id="CAI50315">
        <property type="protein sequence ID" value="CAI50315"/>
        <property type="gene ID" value="NP_4448A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703122"/>
    <dbReference type="KEGG" id="nph:NP_4448A"/>
    <dbReference type="eggNOG" id="arCOG04287">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114656_2_0_2"/>
    <dbReference type="OrthoDB" id="3337at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1410:FF:000002">
        <property type="entry name" value="60S acidic ribosomal protein P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01478">
        <property type="entry name" value="Ribosomal_L12_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027534">
        <property type="entry name" value="Ribosomal_P1/P2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022295">
        <property type="entry name" value="Ribosomal_P1_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03685">
        <property type="entry name" value="ribo_P1_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="59"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="75"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI50315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="116" mass="11656" checksum="69F30AF2A0904AF3" modified="2017-05-10" version="1">MEYVYAALILHESGEELNEDNLTDVLDAAGVDVEESRVKALVAALEDVDVEEAIETAAAVPAGGAGGAAAGGAAEADEAEEADEGGDADEAEEAEEEAEDDGDDEDGGEGLGELFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYL7</accession>
    <name>A0A1U7EYL7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">GTP-binding protein Drg</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">drg</name>
        <name evidence="6" type="ordered locus">NP_4456A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323934.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYL7"/>
    <dbReference type="STRING" id="348780.NP_4456A"/>
    <dbReference type="EnsemblBacteria" id="CAI50319">
        <property type="protein sequence ID" value="CAI50319"/>
        <property type="gene ID" value="NP_4456A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702067"/>
    <dbReference type="KEGG" id="nph:NP_4456A"/>
    <dbReference type="eggNOG" id="arCOG00358">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044997_0_0_2"/>
    <dbReference type="OrthoDB" id="372125at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01896">
        <property type="entry name" value="DRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045001">
        <property type="entry name" value="DRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031662">
        <property type="entry name" value="GTP-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006074">
        <property type="entry name" value="GTP1-OBG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43127">
        <property type="entry name" value="DEVELOPMENTALLY-REGULATED GTP-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16897">
        <property type="entry name" value="MMR_HSR1_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00905">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="4">
        <location>
            <begin position="62"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="5">
        <location>
            <begin position="293"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="34"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI50319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="371" mass="41257" checksum="DACA1C71AB15E021" modified="2017-05-10" version="1">MGLKEEIEAIEEEIANTPYNKSTEQHIGRLKAKLSEKKEKLERRQSSGSGGGGYAVEKHGDATVALVGFPSVGKSTLLNALTNAESETGSYEFTTLDVNPGMLEYRGANLQLLDVPGLIEGAAEGRGGGQAVLSVVRTADLVLFVLSAFEIDQYERLSTELYNNKVRLDTEPVQVNIRKKHKGGINLTTSDRVSLDDETIMGVLRQYEYVNADVTIREDLTIDQLVDALQDNREYLPSAVAVNKVDLIEPNYVETVKENLRAHDIDPEEAVFISAEKEKGLESLKETIWEKLGLIRIYMDKPGRGIDRDEPLILTESENTVDDALQKLGGSFDDRFRFARVTGPSAKHDEQQVGRDHELQDEDVLRIVARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYN3</accession>
    <name>A0A1U7EYN3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">FAD-dependent oxidoreductase (Homolog to geranylgeranyl reductase)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4564A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323988.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYN3"/>
    <dbReference type="STRING" id="348780.NP_4564A"/>
    <dbReference type="EnsemblBacteria" id="CAI50373">
        <property type="protein sequence ID" value="CAI50373"/>
        <property type="gene ID" value="NP_4564A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702099"/>
    <dbReference type="KEGG" id="nph:NP_4564A"/>
    <dbReference type="eggNOG" id="arCOG00570">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024648_2_0_2"/>
    <dbReference type="OrthoDB" id="192187at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011777">
        <property type="entry name" value="Geranylgeranyl_Rdtase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050407">
        <property type="entry name" value="Geranylgeranyl_reductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02032">
        <property type="entry name" value="GG-red-SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685:SF18">
        <property type="entry name" value="DIGERANYLGERANYLGLYCEROPHOSPHOLIPID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685">
        <property type="entry name" value="GERANYLGERANYL DIPHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12831">
        <property type="entry name" value="FAD_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="457" mass="49905" checksum="4E1C98C91EC43ACE" modified="2017-05-10" version="1">MPPESYDIVVVGGGTAGCFAAATAAQNGLDVVLLERKSEEEGGHIACGDAIKGTSTFPDVIDREYLREESFTNEGITLARFENPKGENLDIGFRGNNGAIVDRKRYGEVLLEEADRVGAELHYNTVVQDVIQENGVVRGVQAMRNGDALEYEADVVIDAAGALSLLQDKADLGDATFDTKVNYQQFCSAYREVIEVPEPVEWDDAIVFKPTAELGYLWYFPRTPTEINVGLGFQMNKEPMELVDELKADIRNRPEFEGATVKNKLGAALPTRRPYDSATAPGYMAVGDAAAHVNPCTGGGIPGAAKAGTWAAEAAIEAISDGNADEEAALWEYNRRVQTDFGKRFAAMDLYNIWGGTHDVDELTDIVAAMPGQQLADALALEGTASMSWPLKIKTAVKTFGHWGTLFELKQLNDLATELKGVYDQYPTSPDDFADWRTERDELMDDVYELTGAEPKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYT7</accession>
    <name>A0A1U7EYT7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4612A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324012.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYT7"/>
    <dbReference type="EnsemblBacteria" id="CAI50397">
        <property type="protein sequence ID" value="CAI50397"/>
        <property type="gene ID" value="NP_4612A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702116"/>
    <dbReference type="KEGG" id="nph:NP_4612A"/>
    <dbReference type="eggNOG" id="arCOG04517">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134819_0_0_2"/>
    <dbReference type="OrthoDB" id="165318at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="162" mass="18821" checksum="3600A5DF3E06A8EE" modified="2017-05-10" version="1">MVVFLANRANLQSKRLHDRIQNRLSSEFDAVCRRRTEPRETGPYRVVGTVNPRQFFGEKAYPTTTARIEVGFQLQTGDPHEYYWFNWIEPERQLLVGWHQDDTHDDIGPVHLQVNDSTTAVDHQPADFIDSHPLDVIERRLESLPDAVAAVEWTDGRPARFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYU2</accession>
    <name>A0A1U7EYU2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HTH domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4684A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324047.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYU2"/>
    <dbReference type="EnsemblBacteria" id="CAI50433">
        <property type="protein sequence ID" value="CAI50433"/>
        <property type="gene ID" value="NP_4684A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702141"/>
    <dbReference type="KEGG" id="nph:NP_4684A"/>
    <dbReference type="eggNOG" id="arCOG02756">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130787_0_1_2"/>
    <dbReference type="OrthoDB" id="325082at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25212">
        <property type="entry name" value="HVO_A0114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="142" mass="16342" checksum="C858F68264C9DB90" modified="2017-05-10" version="1">MTENTLKITFQQAREHRQAARERLRQAEAGDTSKPIEQDVRFILNFEEFDDIARLMRTANLELIEAIVSEEPASIRQLAETVDRDYREVHRNLTELESLGVIEFEDDGSRKKPLLRDGAENIDFSIRFPRSPDRGEVPGTSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYV2</accession>
    <name>A0A1U7EYV2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF457 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4708A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324059.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYV2"/>
    <dbReference type="STRING" id="348780.NP_4708A"/>
    <dbReference type="EnsemblBacteria" id="CAI50445">
        <property type="protein sequence ID" value="CAI50445"/>
        <property type="gene ID" value="NP_4708A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702151"/>
    <dbReference type="KEGG" id="nph:NP_4708A"/>
    <dbReference type="eggNOG" id="arCOG02856">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134822_0_0_2"/>
    <dbReference type="OrthoDB" id="241062at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007404">
        <property type="entry name" value="YdjM-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04307">
        <property type="entry name" value="YdjM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="167" mass="17972" checksum="6EFEBA5EA3C477B4" modified="2017-05-10" version="1">MAELLTHVLAAYILATALSWRWRSLTAPYVTVAMAGAVTPDLNRLALLLPGEHVEATLGVPVDWGAFHTLGGTAVVVAVGALLTPARHRRLVAAMLALGALSHHALDMLLFTPTGRSYAVFWPLTQYTPPTPGLYLSTDRWPALAAGGLAAVVWLLDRRREMAAEDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYY6</accession>
    <name>A0A1U7EYY6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UspA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4760A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324083.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYY6"/>
    <dbReference type="STRING" id="348780.NP_4760A"/>
    <dbReference type="EnsemblBacteria" id="CAI50471">
        <property type="protein sequence ID" value="CAI50471"/>
        <property type="gene ID" value="NP_4760A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702168"/>
    <dbReference type="KEGG" id="nph:NP_4760A"/>
    <dbReference type="eggNOG" id="arCOG00451">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101252_0_0_2"/>
    <dbReference type="OrthoDB" id="157328at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="1">
        <location>
            <begin position="151"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="231" mass="24783" checksum="2145940C487917C7" modified="2017-05-10" version="1">MTRVLVPIEVLEGQSVSWGLIELLGTVNVTVLGYHELPEQTPPEQAKEQFEGRANEALSDLETEFRDAGSTVDSRLVFTQDGRSTINRVATEVDADAYTIPGMTGDIARILVPLSGDVAVERILSFVEALLGGRDIGVTLFLASDAEGAGELLDRSATRLREQDISVETRLETGDSPFDALVEAVSGHDAVVMGEKAPSLSSFLFGEETERLATASVGPVLVVRNPDNREA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYZ5</accession>
    <name>A0A1U7EYZ5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transport protein (Probable substrate cationic amino acids)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">cat7</name>
        <name evidence="7" type="synonym">tp02</name>
        <name evidence="7" type="ordered locus">NP_4758A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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        <subcellularLocation>
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    </dbReference>
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    </dbReference>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="87"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <begin position="184"/>
            <end position="207"/>
        </location>
    </feature>
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            <end position="240"/>
        </location>
    </feature>
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            <end position="283"/>
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            <end position="321"/>
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    </feature>
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        <location>
            <begin position="380"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="418"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="444"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI50470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="470" mass="47908" checksum="AD4BD9E8FE38D2AA" modified="2017-05-10" version="1">MDDPPAGVGAEGGPSETVIETDEATVTESAELERTIGLSGGLAIGIGTMIGAGIFVFPGLAAGRAGPAAALSFAIGAVIALLVALPASELATAMPKSGGGYYFISRGLGTLAGTIVGLSLWFGLVFATAFYLVGFGYYAVDALREVGFAVGEGIVIPLALLFGAAFTVLNVTGTENAAKLQNGIVALLLSILAVFLSAGVLTSLGVVGEPSAPEQFLPAGAGIGIVFSTAALVFTSYLGFAQIATVAGEMKSPGRNLPLSMVGSVLVVGVLYVLTIFVATSAFGATRLSELGETAMVAVGRELLGPAGALAIIIGGLLATMSSENASILSTSRAIYAVSRDGLLPSAASRINLKYGTPHVALGMAGGPILALTWTGRVEILAEVASFLHLVMYGLICIALIALRRDEPSWYDPDFRTPGYPVIPILGAVASFALIAFMQPLSRILGIAVIIGTAVWYVYYARDVNLKGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ13</accession>
    <name>A0A1U7EZ13_NATPD</name>
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        <recommendedName>
            <fullName evidence="9">Phosphate transporter</fullName>
        </recommendedName>
    </protein>
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        <name evidence="10" type="primary">tp34</name>
        <name evidence="10" type="ordered locus">NP_4804A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
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            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Potential transporter for phosphate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Membrane</location>
            <topology evidence="2 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family.</text>
    </comment>
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        <property type="protein sequence ID" value="CAI50493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324105.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ13"/>
    <dbReference type="STRING" id="348780.NP_4804A"/>
    <dbReference type="EnsemblBacteria" id="CAI50493">
        <property type="protein sequence ID" value="CAI50493"/>
        <property type="gene ID" value="NP_4804A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703310"/>
    <dbReference type="KEGG" id="nph:NP_4804A"/>
    <dbReference type="eggNOG" id="arCOG02267">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015355_0_0_2"/>
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    <dbReference type="Proteomes" id="UP000002698">
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Phos_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101">
        <property type="entry name" value="PHOSPHATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101:SF80">
        <property type="entry name" value="PHOSPHATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01384">
        <property type="entry name" value="PHO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
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        <location>
            <begin position="130"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
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            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="264"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="367"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI50493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="392" mass="40350" checksum="15C9CB0586150393" modified="2017-05-10" version="1">MVEALLVVGVIASLFVGFNIGGSSTGIAWGPPVGAGIIKKTTAAGLMTVFVFLGGWTVGRNVMDTLSGQIITIELTLSAGVAILFFIGLGILAANIFGVPVPTSMTTVGAIAGLGLATGTLNYPVIAGIISWWVVTPVIGFWIGGIIGRYIYPEVNRRVQIKKSEGPLLTLDRDAAVPKPTFGPNTTWTELISTSAVLVIGCYMAFSAGASNVPNAAAPLVGGASGLPDDTAIILATIAIGVGGFTIARRTMESVGGELSDIPLLAALFVMVTASTITTVLSWFGIPISLVMSTVMTIVGIGWGRATRPVTVSDAVRRDTGDAEIVPGAITAKGMEGKAASPIGEAEPDEVLDAGDLFNPRAVIKYISMWIIGPSISTLLAYGFFRILPGVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ39</accession>
    <name>A0A1U7EZ39_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Probable secreted glycoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_4842A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324124.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ39"/>
    <dbReference type="STRING" id="348780.NP_4842A"/>
    <dbReference type="EnsemblBacteria" id="CAI50512">
        <property type="protein sequence ID" value="CAI50512"/>
        <property type="gene ID" value="NP_4842A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702192"/>
    <dbReference type="KEGG" id="nph:NP_4842A"/>
    <dbReference type="eggNOG" id="arCOG02916">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_472233_0_0_2"/>
    <dbReference type="OrthoDB" id="121941at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="295"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="315"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="577" mass="62294" checksum="96D2A2E77414207C" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ72</accession>
    <name>A0A1U7EZ72_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ubiquitin-like modifier protein SAMP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">samp1</name>
        <name evidence="1" type="ordered locus">NP_5020A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324211.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ72"/>
    <dbReference type="STRING" id="348780.NP_5020A"/>
    <dbReference type="EnsemblBacteria" id="CAI50601">
        <property type="protein sequence ID" value="CAI50601"/>
        <property type="gene ID" value="NP_5020A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702926"/>
    <dbReference type="KEGG" id="nph:NP_5020A"/>
    <dbReference type="eggNOG" id="arCOG00536">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114601_1_2_2"/>
    <dbReference type="OrthoDB" id="98357at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd17505">
        <property type="entry name" value="Ubl_SAMP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010038">
        <property type="entry name" value="MoaD_arc-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054834">
        <property type="entry name" value="SAMP1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052045">
        <property type="entry name" value="Sulfur_Carrier/Prot_Modifier"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003749">
        <property type="entry name" value="ThiS/MoaD-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01687">
        <property type="entry name" value="moaD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041918">
        <property type="entry name" value="SAMP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38031:SF1">
        <property type="entry name" value="SULFUR CARRIER PROTEIN CYSO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38031">
        <property type="entry name" value="SULFUR CARRIER PROTEIN SLR0821-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02597">
        <property type="entry name" value="ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="MoaD/ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="92" mass="10099" checksum="840CA8DFDFED9E76" modified="2017-05-10" version="1">MEWRLFANLAEAAGTKRVEVDAAPGDTFGDAFEQLLAAHPDLEAEVLDEDGELRDHIRVLRNDRNPFVSDDGFETTLEEGDELALFPPVSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZF9</accession>
    <name>A0A1U7EZF9_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HVO-0234-like beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5160A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324281.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZF9"/>
    <dbReference type="STRING" id="348780.NP_5160A"/>
    <dbReference type="EnsemblBacteria" id="CAI50671">
        <property type="protein sequence ID" value="CAI50671"/>
        <property type="gene ID" value="NP_5160A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702276"/>
    <dbReference type="KEGG" id="nph:NP_5160A"/>
    <dbReference type="eggNOG" id="arCOG04726">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082034_0_0_2"/>
    <dbReference type="OrthoDB" id="213812at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056505">
        <property type="entry name" value="Beta-prop_HVO_0234"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23366">
        <property type="entry name" value="Beta-prop_HVO_0234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HVO-0234-like beta-propeller" evidence="1">
        <location>
            <begin position="7"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="258" mass="26281" checksum="E9E5CBBC6040C947" modified="2017-05-10" version="1">MSDEDISIHEKRVYADKSATTTALVASSVGVTEVKISDDIVGEFSLAHRCQARDVATGNGRAAVATEADVLVGVDGEFVDTGFGPASAVGYGDGLVAAGGGRIARYDGDDWEQLSTVENVRAIDGDFAAAAGGVYRLDGTHVGLDDAYDVVGGAEPLAATGSGLYYLANGWMEAADGSFHAVAGGSSTAAHAVGDGTLFEQNEEWTAVELPTEESVVDVAHGDGAYAVTETGTVLATVGDGWRHRALGLPDVSGLAVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZH3</accession>
    <name>A0A1U7EZH3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5270A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324335.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZH3"/>
    <dbReference type="EnsemblBacteria" id="CAI50726">
        <property type="protein sequence ID" value="CAI50726"/>
        <property type="gene ID" value="NP_5270A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702313"/>
    <dbReference type="KEGG" id="nph:NP_5270A"/>
    <dbReference type="eggNOG" id="arCOG06301">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087822_0_0_2"/>
    <dbReference type="OrthoDB" id="206283at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="226" mass="24615" checksum="4C515B40612B613A" modified="2017-05-10" version="1">MRRRTLLVSAAALPAVAGCSNVLPTGTNDQPQTPHEESPRVDTPPHEINRPDPPDGPPGERDWNDDYLGETMPTEPSLAFEPVSARLTERYRLSHDLAPAAYRVGLVDDASALSDHFALDRMDDADRDAVEAVDFDTHVLAVVVSGFGSGSVSHRWARAEADDDGIHLHGYYSAPLVRTDDYTIRTSALQIERPADGVGFIRVSLTTDETTRVHFNSTEGAVSVDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZJ0</accession>
    <name>A0A1U7EZJ0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KEOPS complex subunit Pcc1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pcc1</name>
        <name evidence="2" type="ordered locus">NP_5236A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CTAG/PCC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324319.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZJ0"/>
    <dbReference type="STRING" id="348780.NP_5236A"/>
    <dbReference type="EnsemblBacteria" id="CAI50709">
        <property type="protein sequence ID" value="CAI50709"/>
        <property type="gene ID" value="NP_5236A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702306"/>
    <dbReference type="KEGG" id="nph:NP_5236A"/>
    <dbReference type="eggNOG" id="arCOG04414">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170076_1_0_2"/>
    <dbReference type="OrthoDB" id="8982at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015419">
        <property type="entry name" value="CTAG/Pcc1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011470">
        <property type="entry name" value="PRK14887.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09341">
        <property type="entry name" value="Pcc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="86" mass="9361" checksum="C13444A5BDB5A1CF" modified="2017-05-10" version="1">MHTAELVFEYETSERATHIAAAIRPEVGDIQGDRTTASLSRDDARITVDIEADDLVALRAGLNTWSTLLEAAERVNESGSRCRSGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZN0</accession>
    <name>A0A1U7EZN0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5338A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324369.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZN0"/>
    <dbReference type="STRING" id="348780.NP_5338A"/>
    <dbReference type="EnsemblBacteria" id="CAI50760">
        <property type="protein sequence ID" value="CAI50760"/>
        <property type="gene ID" value="NP_5338A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702339"/>
    <dbReference type="KEGG" id="nph:NP_5338A"/>
    <dbReference type="eggNOG" id="arCOG09042">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1131643_0_0_2"/>
    <dbReference type="OrthoDB" id="271889at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="246" mass="27253" checksum="CD503C953F5702A3" modified="2017-05-10" version="1">MSTIVTKKVDGYGPYAYRVTYTGDGHHWEYLGPAGKVDPAELTDKETAELRAEGFALARYRETTTAEFGDLETANAVRDELPDGVLAPTDDRRDTTIEVLGDELDADERAYLEGAAADSKRRETYQHGQAELTDAERRDVDPDNLMHARSAKAILQGEGVDDWRAYYDPELTPSEHVDVAERARRDESGARADREGARETRRAADQFRAAQGAMADHAREACREGHEEACAELREMGIEPPEPAGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7F3L4</accession>
    <name>A0A1U7F3L4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3894B</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CEK03539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7F3L4"/>
    <dbReference type="EnsemblBacteria" id="CEK03539">
        <property type="protein sequence ID" value="CEK03539"/>
        <property type="gene ID" value="NP_3894B"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3894B"/>
    <dbReference type="HOGENOM" id="CLU_2893139_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CEK03539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="65" mass="7437" checksum="E871E079CB7E6B47" modified="2017-05-10" version="1">MNENEIDPETYVEKNSDQLVYIIKHSNDRFIRGLCLAALVKYGGEKVGKDQIEKELAQLRDVEGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILW2</accession>
    <name>Q3ILW2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glutamine amidotransferase (Homolog to GMP synthase subunit A)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">guaAa3</name>
        <name evidence="2" type="ordered locus">NP_6256A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324505.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILW2"/>
    <dbReference type="EnsemblBacteria" id="CAI50908">
        <property type="protein sequence ID" value="CAI50908"/>
        <property type="gene ID" value="NP_6256A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694687"/>
    <dbReference type="KEGG" id="nph:NP_6256A"/>
    <dbReference type="HOGENOM" id="CLU_054974_4_1_2"/>
    <dbReference type="OrthoDB" id="7388at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01741">
        <property type="entry name" value="GATase1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044992">
        <property type="entry name" value="ChyE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42695">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE YLR126C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42695:SF5">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE YLR126C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="1">
        <location>
            <begin position="26"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="243" mass="27160" checksum="56415299548D2C84" modified="2005-11-08" version="1">MDQPSLALLNAAHKAEETKENFHRELDADLETFHCPTGEFPDDFQYDGFVVTGSRASVYWDREWIGQLKTWVGDAAEAGLPGLGVCFGHQLLADVLGGRVEGMGEYELGYRTIEQDGKNRLLEDIDETLTVFTSHSDHVREKPPGATVFARNEYGIQGFRKHNIFAVQFHPEYDMETARMITKGKSGDLSPQQIDAVLDGIHSTNYEAACEAKQLFDNFLSYVRDIQEVGRVGKSGDVPETEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILW7</accession>
    <name>Q3ILW7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DUF234 domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_6246A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324500.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILW7"/>
    <dbReference type="EnsemblBacteria" id="CAI50903">
        <property type="protein sequence ID" value="CAI50903"/>
        <property type="gene ID" value="NP_6246A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694653"/>
    <dbReference type="KEGG" id="nph:NP_6246A"/>
    <dbReference type="HOGENOM" id="CLU_041137_3_0_2"/>
    <dbReference type="OrthoDB" id="132045at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004256">
        <property type="entry name" value="DUF234"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34704">
        <property type="entry name" value="ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34704:SF1">
        <property type="entry name" value="ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03008">
        <property type="entry name" value="DUF234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="DUF234" evidence="2">
        <location>
            <begin position="310"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="463" mass="52679" checksum="43D2A8FA8B058D6F" modified="2005-11-08" version="1">MTFYDREAELDTLSDAVESPGADFVVVYGRRRVGKTELLKEFCANRPHIYFLAAQEAEHRQREKFLDQVADYFDERVPRIDGWDEAFEYLGEQLQREDLVVVIDEFPYLVAENDSLPSYVQGFVDQELDGTDSMIVLCGSSVSTMESEILGHESPLYGRRTAQLDVTPFSFQQAREVISYDIQDAIRSYAVTGGTPMYLTLFDYTQSLAANIQSHVLSPSAVLYNEPEFLLRTELRNPARYMSILEAVALGHTTPNEISGATGIDSGPLSKYLQTLRRLRLIERDVPVMASGKKSKRSRYRVADEFLRFWFRYVEPNRSSIEEAPEIVYDGTIEPDLPTHVATTFEDVCQEAVWEGIRRGTFDPYSEVGRWWYGEEEIDIVGLAPNDDRVLLAECKWTTDPVGEDLVESLRAKAEHVRWGPPDRDEQFALFSKSGFVDGLEAQLDDSWSLFTVADIDDLLTPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM53</accession>
    <name>Q3IM53_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Transport protein (Probable substrate arsenite)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">tp26</name>
        <name evidence="10" type="ordered locus">NP_6058A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10 11">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the arsenical resistance-3 (ACR3) (TC 2.A.59) family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50809.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939907.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM53"/>
    <dbReference type="EnsemblBacteria" id="CAI50809">
        <property type="protein sequence ID" value="CAI50809"/>
        <property type="gene ID" value="NP_6058A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694605"/>
    <dbReference type="KEGG" id="nph:NP_6058A"/>
    <dbReference type="HOGENOM" id="CLU_022869_0_0_2"/>
    <dbReference type="OrthoDB" id="326456at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015104">
        <property type="term" value="F:antimonite transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015105">
        <property type="term" value="F:arsenite transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046685">
        <property type="term" value="P:response to arsenic-containing substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1530.20:FF:000009">
        <property type="entry name" value="Arsenite transporter, ACR3 family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004706">
        <property type="entry name" value="Arsenical-R_Acr3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002657">
        <property type="entry name" value="BilAc:Na_symport/Acr3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00832">
        <property type="entry name" value="acr3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43057:SF1">
        <property type="entry name" value="ARSENICAL-RESISTANCE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43057">
        <property type="entry name" value="ARSENITE EFFLUX TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01758">
        <property type="entry name" value="SBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005508">
        <property type="entry name" value="Acr3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0059">Arsenical resistance</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="158"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="245"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="275"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="309"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="336"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI50809.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="393" mass="42717" checksum="5F41909438696C91" modified="2012-10-03" version="2">MRDAGHEHGPDCGCESCGDPRSMDFLDKYLTVWIFLAMGVGVGLGYVAPSIVAPIQEYYLVEIGLIAMMYPPLAKVNYRQLPRVFSAWRVLSLSLIQNWLIGPTLMFLLAVVFFSGLVPPFPAHPEYFLGLIFIGMARCIAMVLVWNDLADGSSEYAAGLVAFNSVFQIITYGVYIWFFALFLPPLLGMDALAAGIGTFDITVTQVFWAIAIFLGIPFAGGILTRLGGVRTKGETWYEEEFVPKISPVTLLALLFTVIVMFATQGDNILAQPTDVLWIAVPLTIYFIIMFLVSFAMGRGIGADYSTTTAIGFTAASNNFELAIAVSVAVFGVGSGVAFATVIGPLIEVPVLLALVNVAIYFQQRFDWAGYETGQIERPASAAEEQSPPSTNDD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMF0</accession>
    <name>RPO12_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo12</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit P</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo12</name>
        <name evidence="1" type="synonym">rpoP</name>
        <name type="ordered locus">NP_5234A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal Rpo12/eukaryotic RPC10 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324318.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMF0"/>
    <dbReference type="SMR" id="Q3IMF0"/>
    <dbReference type="STRING" id="348780.NP_5234A"/>
    <dbReference type="EnsemblBacteria" id="CAI50708">
        <property type="protein sequence ID" value="CAI50708"/>
        <property type="gene ID" value="NP_5234A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703164"/>
    <dbReference type="KEGG" id="nph:NP_5234A"/>
    <dbReference type="eggNOG" id="arCOG04341">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_179456_2_1_2"/>
    <dbReference type="OrthoDB" id="129238at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.30">
        <property type="entry name" value="RNA polymerase ii, chain L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00615">
        <property type="entry name" value="RNApol_arch_Rpo12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006591">
        <property type="entry name" value="RNAP_P/RPABC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029040">
        <property type="entry name" value="RPABC4/Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023464">
        <property type="entry name" value="Rpo12"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001606">
        <property type="entry name" value="PRK00398.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03604">
        <property type="entry name" value="Zn_ribbon_RPAB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00659">
        <property type="entry name" value="RPOLCX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63393">
        <property type="entry name" value="RNA polymerase subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_1000061340" description="DNA-directed RNA polymerase subunit Rpo12">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00615"/>
        </source>
    </evidence>
    <sequence length="44" mass="5236" checksum="DCB5F69AA4CACD3C" modified="2005-11-08" version="1">MSYKCSRCKRDVELDEYGGVRCPYCGHRVLLKERSRDIKEVEVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMK4</accession>
    <name>Q3IMK4_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Probable cobyric acid synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">cbiP</name>
        <name evidence="7 10" type="synonym">cobQ</name>
        <name evidence="10" type="ordered locus">NP_5126A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the CobB/CobQ family. CobQ subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50654.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMK4"/>
    <dbReference type="STRING" id="348780.NP_5126A"/>
    <dbReference type="EnsemblBacteria" id="CAI50654">
        <property type="protein sequence ID" value="CAI50654"/>
        <property type="gene ID" value="NP_5126A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_5126A"/>
    <dbReference type="eggNOG" id="arCOG00105">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019250_2_2_2"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015420">
        <property type="term" value="F:ABC-type vitamin B12 transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01750">
        <property type="entry name" value="GATase1_CobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00028">
        <property type="entry name" value="CobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033949">
        <property type="entry name" value="CobQ_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004459">
        <property type="entry name" value="CobQ_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011698">
        <property type="entry name" value="GATase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00313">
        <property type="entry name" value="cobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001989">
        <property type="entry name" value="PRK00784.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21343:SF1">
        <property type="entry name" value="COBYRIC ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21343">
        <property type="entry name" value="DETHIOBIOTIN SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07685">
        <property type="entry name" value="GATase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51274">
        <property type="entry name" value="GATASE_COBBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="10" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobQ/CobB/MinD/ParA nucleotide binding" evidence="8">
        <location>
            <begin position="8"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="CobB/CobQ-like glutamine amidotransferase" evidence="9">
        <location>
            <begin position="269"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="344"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI50654.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="503" mass="52272" checksum="863C995DBE241F7C" modified="2012-10-31" version="2">MSRARTVLVAGTASHVGKSTVAAGLCRLLSDSGVSVAPFKAQNMSNNARAVAAATGSGFGEIGVSQYVQARAAGVAATTDHNPVLLKPRGSGESQLVIDGAAAGNFAAGEYYETGWERARAAAERAHERLASDHDVIVAEGAGSIAEINLQDRDLANIETARFADADIVLVADIERGGVFAAIVGTVELLPEDIRERVAGVVITKFRGDVSLLEPGLETLEAKTGVPVLGVLPYDDPGLPEEDSVSLPAADESATLGDDDGVADGRTVTVAVPRFPRASNVTDIEPLARVPGVRVRFVPLDAPLSADAVVLTGTKNTVDDALAAREAGLYDRLRAFDGPVVGICGGYQLLGERLLDADIEGTGDTETVPGVGLLPVETRFHPEKTVEPVTRTLDGCGPLAGASGPVSGYEIHMGQSRLLSPVDRPFEERGAATDAVLGVYLHGLFENRLARAAFVRAVYASAGRSRPEADTPDRSPYDRAAALVSELDLAPLGLAAESLSSDD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMS0</accession>
    <name>G6PI_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable glucose-6-phosphate isomerase</fullName>
            <shortName evidence="1">GPI</shortName>
            <ecNumber evidence="1">5.3.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Phosphoglucose isomerase</fullName>
            <shortName evidence="1">PGI</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphohexose isomerase</fullName>
            <shortName evidence="1">PHI</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pgi</name>
        <name type="ordered locus">NP_4992A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible isomerization of glucose-6-phosphate to fructose-6-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11816"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="EC" id="5.3.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GPI family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.9" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324198.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMS0"/>
    <dbReference type="SMR" id="Q3IMS0"/>
    <dbReference type="STRING" id="348780.NP_4992A"/>
    <dbReference type="EnsemblBacteria" id="CAI50587">
        <property type="protein sequence ID" value="CAI50587"/>
        <property type="gene ID" value="NP_4992A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703019"/>
    <dbReference type="KEGG" id="nph:NP_4992A"/>
    <dbReference type="eggNOG" id="arCOG00052">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037303_1_0_2"/>
    <dbReference type="OrthoDB" id="168618at2157"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00181"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004347">
        <property type="term" value="F:glucose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051156">
        <property type="term" value="P:glucose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05015">
        <property type="entry name" value="SIS_PGI_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00473">
        <property type="entry name" value="G6P_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001672">
        <property type="entry name" value="G6P_Isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018189">
        <property type="entry name" value="Phosphoglucose_isomerase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035476">
        <property type="entry name" value="SIS_PGI_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469:SF1">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00342">
        <property type="entry name" value="PGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00662">
        <property type="entry name" value="G6PISOMERASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00765">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00174">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51463">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0312">Gluconeogenesis</keyword>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000230944" description="Probable glucose-6-phosphate isomerase">
        <location>
            <begin position="1"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="269"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="290"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00473"/>
        </source>
    </evidence>
    <sequence length="428" mass="44803" checksum="79194EECAC97C7AE" modified="2005-11-08" version="1">MNVDFGNALAAEAVTGVSEASLERLDDRVADAHDRIEANIEARRFGYASLALPTDTDPDAIYEAVSGFDPEAVLTVGIGGSALGAETITAALGAESHYTLDNVDPAPTRQLLDELPLSSTLVNVVSRSGTTAETLANFLVVREAMADAGVDWTDRTVVTTGAEGPLRTLADAHDLPSCTVPEGVPGRFSALSAVGLLPAAALGCDIEAVLAGGAAGRESLAPSLFESPAYAYGAVAYATEQRGATTNAIVPYAEQLEPFAEWFAQLWAESLGKDGLGQTPARALGATDQHSQLQLYRAGRKDKLVTLVRPRERAGVDIPETDIDALSYLGGESLESLLDAEFEATEASLAAAGQPNVRIELDSLDAHGVGELLYGMEAACILYGELLGIETFTQPAVEWGKRAARGLLGGGDFEEAEAVADKTVRRVE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMW0</accession>
    <name>KAD_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Adenylate kinase</fullName>
            <shortName evidence="1">AK</shortName>
            <ecNumber evidence="1">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP-AMP transphosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">ATP:AMP phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Adenylate monophosphate kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">adk1</name>
        <name type="ordered locus">NP_4910A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from ADP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and disassembling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the adenylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324158.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMW0"/>
    <dbReference type="SMR" id="Q3IMW0"/>
    <dbReference type="STRING" id="348780.NP_4910A"/>
    <dbReference type="EnsemblBacteria" id="CAI50546">
        <property type="protein sequence ID" value="CAI50546"/>
        <property type="gene ID" value="NP_4910A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702493"/>
    <dbReference type="KEGG" id="nph:NP_4910A"/>
    <dbReference type="eggNOG" id="arCOG01046">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032354_6_1_2"/>
    <dbReference type="OrthoDB" id="31230at2157"/>
    <dbReference type="UniPathway" id="UPA00588">
        <property type="reaction ID" value="UER00649"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044209">
        <property type="term" value="P:AMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00235">
        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006259">
        <property type="entry name" value="Adenyl_kin_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01351">
        <property type="entry name" value="adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011103">
        <property type="entry name" value="PRK14530.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000021749" description="Adenylate kinase">
        <location>
            <begin position="1"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="NMP" evidence="1">
        <location>
            <begin position="33"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="1">
        <location>
            <begin position="120"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="13"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="59"/>
            <end position="61"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="84"/>
            <end position="87"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="130"/>
            <end position="131"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50546.1"/>
        </source>
    </evidence>
    <sequence length="206" mass="22957" checksum="EB0F4EF24168823A" modified="2005-11-08" version="1">MSQPKILLLGAPGAGKGTQSSNIVDEYGVDHITTGDALRANKDMETEHGTPREFMEAGELVPDPVVNEIVQAAIDEADGFVLDGYPRNLSQAEYLSDITDVDVVALLDVGRDELVDRLTGRRMDPETGDIYHTEFNMPDDEEVRERLVQRDDDTEETVNERLDVFDENTQPVIDYYEDEGELVRIDGEASPDEVWDDLQAAIDDAL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q3INQ6</accession>
    <name>RL37_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein eL37</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L37e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl37e</name>
        <name type="ordered locus">NP_4310A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL37 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323862.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3INQ6"/>
    <dbReference type="SMR" id="Q3INQ6"/>
    <dbReference type="STRING" id="348780.NP_4310A"/>
    <dbReference type="EnsemblBacteria" id="CAI50246">
        <property type="protein sequence ID" value="CAI50246"/>
        <property type="gene ID" value="NP_4310A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703143"/>
    <dbReference type="KEGG" id="nph:NP_4310A"/>
    <dbReference type="eggNOG" id="arCOG04126">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_208825_0_0_2"/>
    <dbReference type="OrthoDB" id="5619at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.30:FF:000003">
        <property type="entry name" value="50S ribosomal protein L37e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00547">
        <property type="entry name" value="Ribosomal_eL37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001569">
        <property type="entry name" value="Ribosomal_eL37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_eL37/eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018267">
        <property type="entry name" value="Ribosomal_eL37_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003214">
        <property type="entry name" value="PRK04179.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10768">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10768:SF0">
        <property type="entry name" value="RIBOSOMAL PROTEIN L37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01907">
        <property type="entry name" value="Ribosomal_L37e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01077">
        <property type="entry name" value="RIBOSOMAL_L37E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_1000017770" description="Large ribosomal subunit protein eL37">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="57" mass="6349" checksum="64902F0CA9628009" modified="2005-11-08" version="1">MTGAGTPSQGKKNKTTHVKCRRCGEKSYHKQKKVCASCGFGKSAKRRDYAWQSKQGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q3INZ6</accession>
    <name>PGK_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoglycerate kinase</fullName>
            <ecNumber evidence="1">2.7.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pgk</name>
        <name type="ordered locus">NP_4130A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:14801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphoglycerate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.3" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323772.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3INZ6"/>
    <dbReference type="SMR" id="Q3INZ6"/>
    <dbReference type="STRING" id="348780.NP_4130A"/>
    <dbReference type="EnsemblBacteria" id="CAI50156">
        <property type="protein sequence ID" value="CAI50156"/>
        <property type="gene ID" value="NP_4130A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703020"/>
    <dbReference type="KEGG" id="nph:NP_4130A"/>
    <dbReference type="eggNOG" id="arCOG00496">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025427_0_2_2"/>
    <dbReference type="OrthoDB" id="6575at2157"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00185"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004618">
        <property type="term" value="F:phosphoglycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1260:FF:000006">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1260:FF:000012">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1260">
        <property type="entry name" value="Phosphoglycerate kinase, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00145">
        <property type="entry name" value="Phosphoglyc_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001576">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015824">
        <property type="entry name" value="Phosphoglycerate_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036043">
        <property type="entry name" value="Phosphoglycerate_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406">
        <property type="entry name" value="PHOSPHOGLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406:SF23">
        <property type="entry name" value="PHOSPHOGLYCERATE KINASE 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00162">
        <property type="entry name" value="PGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000724">
        <property type="entry name" value="Pgk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00477">
        <property type="entry name" value="PHGLYCKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53748">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000192862" description="Phosphoglycerate kinase">
        <location>
            <begin position="1"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="20"/>
            <end position="22"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="58"/>
            <end position="61"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="353"/>
            <end position="356"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00145"/>
        </source>
    </evidence>
    <sequence length="396" mass="42316" checksum="FCAB17DBE8F414FA" modified="2005-11-08" version="1">MIRTLDDLEADGTALGVRIDINSPLSSDGLADDARLRAHVDTVEELCRRDARVALLAHQGRPGGDEFSDLERHAERLDELLDAPVEYCDSTFSAEARTRIDELDPGRAVLLENTRFYSEEYMSFEPSAAAETYLVSRLAPALDAYVNDAFATAHRSQPSVVGFPERLPAYAGRVMERELDVLGNIESSPEPRVYVLGGAKVDDSIAVARSVLERGLADSVLTAGIVGNAFLLADGVSLGAASAAVVNERSHEAVKQAGDLLDDFSHRIYMPRDVAVENDAGERVEHDLEDLPASTPAMDIGARTVAAYANILDDAGTAILNGPAGVFEDDRFETGTLELYKSATRAEQSIVGGGDTASALRKLGILEDFDHVSTGGGAALNMLTGETLVGVEALRE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-04-05" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IQ95</accession>
    <name>RIBL_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAD synthase</fullName>
            <ecNumber evidence="1">2.7.7.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">FMN adenylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Flavin adenine dinucleotide synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ribL</name>
        <name type="ordered locus">NP_3220A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of the AMP portion of ATP to flavin mononucleotide (FMN) to produce flavin adenine dinucleotide (FAD) coenzyme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>FMN + ATP + H(+) = FAD + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:17237"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="2.7.7.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal FAD synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.2" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323323.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IQ95"/>
    <dbReference type="SMR" id="Q3IQ95"/>
    <dbReference type="STRING" id="348780.NP_3220A"/>
    <dbReference type="EnsemblBacteria" id="CAI49701">
        <property type="protein sequence ID" value="CAI49701"/>
        <property type="gene ID" value="NP_3220A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701688"/>
    <dbReference type="KEGG" id="nph:NP_3220A"/>
    <dbReference type="eggNOG" id="arCOG01222">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034585_2_1_2"/>
    <dbReference type="OrthoDB" id="1912at2157"/>
    <dbReference type="UniPathway" id="UPA00277">
        <property type="reaction ID" value="UER00407"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003919">
        <property type="term" value="F:FMN adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006747">
        <property type="term" value="P:FAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046444">
        <property type="term" value="P:FMN metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02115">
        <property type="entry name" value="FAD_synth_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050385">
        <property type="entry name" value="Archaeal_FAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024902">
        <property type="entry name" value="FAD_synth_RibL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43793">
        <property type="entry name" value="FAD SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43793:SF1">
        <property type="entry name" value="FAD SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0288">FMN</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000406277" description="FAD synthase">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="9"/>
            <end position="10"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="14"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02115"/>
        </source>
    </evidence>
    <sequence length="140" mass="15806" checksum="B9CB174A8BCDD761" modified="2005-11-08" version="1">MRRVVAQGTFDLLHPGHLYYLTEAKSMGDELHVIVARSQNVTHKQPPIVPDEQRREMVAGLDPVDSARLGHTDDFFVPIRDIDPDVIVLGHDQHHDDERLAEMLTEEGIDCEVARASARDGDDKILSTGRIVDRICEERC</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IUI1</accession>
    <name>GRPE_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein GrpE</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">HSP-70 cofactor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">grpE</name>
        <name type="ordered locus">NP_0216A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GrpE family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321838.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IUI1"/>
    <dbReference type="SMR" id="Q3IUI1"/>
    <dbReference type="STRING" id="348780.NP_0216A"/>
    <dbReference type="EnsemblBacteria" id="CAI48199">
        <property type="protein sequence ID" value="CAI48199"/>
        <property type="gene ID" value="NP_0216A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702777"/>
    <dbReference type="KEGG" id="nph:NP_0216A"/>
    <dbReference type="eggNOG" id="arCOG04772">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057217_3_0_2"/>
    <dbReference type="OrthoDB" id="372230at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00446">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.22.10">
        <property type="entry name" value="Head domain of nucleotide exchange factor GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01151">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000740">
        <property type="entry name" value="GrpE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013805">
        <property type="entry name" value="GrpE_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009012">
        <property type="entry name" value="GrpE_head"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21237">
        <property type="entry name" value="GRPE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21237:SF23">
        <property type="entry name" value="GRPE PROTEIN HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01025">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00773">
        <property type="entry name" value="GRPEPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58014">
        <property type="entry name" value="Coiled-coil domain of nucleotide exchange factor GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51064">
        <property type="entry name" value="Head domain of nucleotide exchange factor GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0346">Stress response</keyword>
    <feature type="chain" id="PRO_1000053607" description="Protein GrpE">
        <location>
            <begin position="1"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="193"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="136"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="204"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="217" mass="23817" checksum="BC8E333A2BE12A37" modified="2005-11-08" version="1">MSDDQGDAVEASSEADEEASTSPDEGDDRDPVAAVAERAENDPASVAAELVALREEAAELETERDDLESRLKRKQAEFQNYKKRQEKQREKERARATEALVEKLLEVRDNLNRALEQDADADIREGVEATFRQLDDILDGEGVEAIEPDPGTETDPKRHEVLLQVESDEPEGTVAELHRPGYEMAGKVLRAAQVTVSEGPSGDSEAEDDADGEDGDE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IUM4</accession>
    <name>EF2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Elongation factor 2</fullName>
            <shortName evidence="1">EF-2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fusA</name>
        <name type="ordered locus">NP_0130A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321795.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IUM4"/>
    <dbReference type="SMR" id="Q3IUM4"/>
    <dbReference type="STRING" id="348780.NP_0130A"/>
    <dbReference type="EnsemblBacteria" id="CAI48156">
        <property type="protein sequence ID" value="CAI48156"/>
        <property type="gene ID" value="NP_0130A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703232"/>
    <dbReference type="KEGG" id="nph:NP_0130A"/>
    <dbReference type="eggNOG" id="arCOG01559">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002794_11_1_2"/>
    <dbReference type="OrthoDB" id="6290at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="entry name" value="aeEF2_snRNP_like_IV"/>
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        <property type="entry name" value="EF2"/>
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        <property type="entry name" value="EF2_snRNP_III"/>
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    </dbReference>
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        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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    </dbReference>
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        <property type="entry name" value="EFG_II"/>
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        <property type="entry name" value="EFG_III/V"/>
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    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
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        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
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        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
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        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004543">
        <property type="entry name" value="Transl_elong_EFG/EF2_arc"/>
    </dbReference>
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        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
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        <property type="entry name" value="aEF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF3">
        <property type="entry name" value="ELONGATION FACTOR-LIKE GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0251">Elongation factor</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000225254" description="Elongation factor 2">
        <location>
            <begin position="1"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="domain" description="tr-type G">
        <location>
            <begin position="19"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="28"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="94"/>
            <end position="98"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="148"/>
            <end position="151"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Diphthamide" evidence="1">
        <location>
            <position position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00054"/>
        </source>
    </evidence>
    <sequence length="729" mass="80763" checksum="DB16EDBBA3E08CA1" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETG4</accession>
    <name>A0A1U7ETG4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Peptidase M20 family protein (Homolog to succinyl-diaminopimelate desuccinylase)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_0200A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETG4"/>
    <dbReference type="STRING" id="348780.NP_0200A"/>
    <dbReference type="EnsemblBacteria" id="CAI48191">
        <property type="protein sequence ID" value="CAI48191"/>
        <property type="gene ID" value="NP_0200A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_0200A"/>
    <dbReference type="eggNOG" id="arCOG01107">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1118240_0_0_2"/>
    <dbReference type="OrthoDB" id="133929at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050072">
        <property type="entry name" value="Peptidase_M20A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808">
        <property type="entry name" value="ACETYLORNITHINE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="3">
        <location>
            <begin position="51"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="248" mass="25093" checksum="9F71FFD5C5991A54" modified="2017-05-10" version="1">MESLELASFVGEEDGGVGCRAAIENGFAPEFAVVGEGSTGYSAAGVTDVAVAHKGRRGATLVATGDAAHASEADAATNAIYRATDAVAVVRELDAPETRVFGERLAGSATVTEIDGGEAWNVVPDRCTVTVDERTVPGERAALEAAAVDGVTLDIEQDLPPMACSDDGFAETALAAADAAQKGDAEAVTKPHATDAGWLASRAGTACVVCGAAEPGEAHTDTESVSAAAVERCVDIYRRVAEAVDAEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETM8</accession>
    <name>A0A1U7ETM8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">tRNA (Adenine-N(1))-methyltransferase TrmI</fullName>
            <ecNumber evidence="6">2.1.1.219</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">trmI</name>
        <name evidence="6" type="ordered locus">NP_0356A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.219" evidence="6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48269.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETM8"/>
    <dbReference type="STRING" id="348780.NP_0356A"/>
    <dbReference type="EnsemblBacteria" id="CAI48269">
        <property type="protein sequence ID" value="CAI48269"/>
        <property type="gene ID" value="NP_0356A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_0356A"/>
    <dbReference type="eggNOG" id="arCOG00978">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025402_0_2_2"/>
    <dbReference type="OrthoDB" id="30774at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031515">
        <property type="term" value="C:tRNA (m1A) methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043827">
        <property type="term" value="F:tRNA (adenine(57)-N1)/(adenine(58)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160107">
        <property type="term" value="F:tRNA (adenine(58)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049470">
        <property type="entry name" value="TRM61_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014816">
        <property type="entry name" value="tRNA_MeTrfase_Gcd14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12133">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12133:SF1">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08704">
        <property type="entry name" value="GCD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51620">
        <property type="entry name" value="SAM_TRM61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61 C-terminal" evidence="5">
        <location>
            <begin position="68"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI48269.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="243" mass="25956" checksum="5E76DD0089B33EFA" modified="2017-05-10" version="1">MLVVRGNREFLLEPGATQETDLGVLELPEDIEPGTTVETHLDESFEVRRLRGPDLFDHLERTGAPMMPRDIGLVVGHTGVAAGDRALDAGTGTGVLAAYLGRLGVEVTTYERDPEFAEVARENMETAGVADNVTVETGDIAATIDDLKGPFDLITLDTGDAPEVVAQADDLLASGGFIATYSPFVETARACAEAAREAGLDDVETLETIQREMDFDDRGSRPSTAGVGHTGYLTFARFLPSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU08</accession>
    <name>A0A1U7EU08_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0666A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322060.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU08"/>
    <dbReference type="STRING" id="348780.NP_0666A"/>
    <dbReference type="EnsemblBacteria" id="CAI48424">
        <property type="protein sequence ID" value="CAI48424"/>
        <property type="gene ID" value="NP_0666A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700922"/>
    <dbReference type="KEGG" id="nph:NP_0666A"/>
    <dbReference type="eggNOG" id="arCOG06313">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165238_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058716">
        <property type="entry name" value="WNWW_dom-containing"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26484">
        <property type="entry name" value="WNWW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="97" mass="10594" checksum="F01C46B090446776" modified="2017-05-10" version="1">MAYEHDYFDTAALVAGLETFGGSETERRAVARHARDLADSGQYAADADVKLTPEHVVVQLADAPDGGPADRWNWWVGSLAVAYGDTYATFQIRQFGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU44</accession>
    <name>A0A1U7EU44_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">3-oxoacyl-CoA reductase (NADP)</fullName>
            <ecNumber evidence="7">1.1.1.100</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">fabG</name>
        <name evidence="7" type="ordered locus">NP_0726A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.100" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322090.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU44"/>
    <dbReference type="STRING" id="348780.NP_0726A"/>
    <dbReference type="EnsemblBacteria" id="CAI48454">
        <property type="protein sequence ID" value="CAI48454"/>
        <property type="gene ID" value="NP_0726A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700945"/>
    <dbReference type="KEGG" id="nph:NP_0726A"/>
    <dbReference type="eggNOG" id="arCOG01259">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_2"/>
    <dbReference type="OrthoDB" id="194879at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05333">
        <property type="entry name" value="BKR_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000115">
        <property type="entry name" value="3-oxoacyl-[acyl-carrier-protein] reductase FabG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011284">
        <property type="entry name" value="3oxo_ACP_reduc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050259">
        <property type="entry name" value="SDR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01830">
        <property type="entry name" value="3oxo_ACP_reduc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005559">
        <property type="entry name" value="PRK07231.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009466">
        <property type="entry name" value="PRK12826.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42879">
        <property type="entry name" value="3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42879:SF2">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] REDUCTASE FABG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0521">NADP</keyword>
    <keyword evidence="3 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketoreductase" evidence="6">
        <location>
            <begin position="5"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="11"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="154"/>
            <end position="158"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR611284-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR611284-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="246" mass="26545" checksum="F13A26E5F3A5A1A6" modified="2017-05-10" version="1">MLEDQTCLVTGGSRGIGRAVAEELGRNGADVAINYRSSEAAAEATAEAVREEGGNAITVQADISDYEQVEAMRETVTAEFGPLDVLVNNAGITIDKKFENMTKNDWDRVIDVNLGGAFNCTHCFFEDIKSAENGRLINISSVVGQQGNYGQANYATTKSGLFGFTRTLALELASEGSTANCIAPGFVETDMLETVPERVQEKILRRIPLNRFARPEDVSGIVRFVASPDSSYMTGQVIAVNGGMEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU79</accession>
    <name>A0A1U7EU79_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0856A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322155.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU79"/>
    <dbReference type="STRING" id="348780.NP_0856A"/>
    <dbReference type="EnsemblBacteria" id="CAI48519">
        <property type="protein sequence ID" value="CAI48519"/>
        <property type="gene ID" value="NP_0856A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700978"/>
    <dbReference type="KEGG" id="nph:NP_0856A"/>
    <dbReference type="eggNOG" id="arCOG03097">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148450_0_0_2"/>
    <dbReference type="OrthoDB" id="137027at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043900">
        <property type="entry name" value="DUF5788"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19101">
        <property type="entry name" value="DUF5788"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="146" mass="17077" checksum="51782CB86B54E970" modified="2017-05-10" version="1">MQEYQRKQLLERIDREGATLGVDIPDRIEIQGEEVELREFVFEIKRRETVPAGERERVEQAKRNLRRERLERKERIEEGEIPFEEGERLADAIVGIDRALEALESLGPESVEAEAKRQQAADKKRWMDFLKKALGHDDDGPQVGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU99</accession>
    <name>A0A1U7EU99_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ribonuclease R</fullName>
            <ecNumber evidence="3">3.1.13.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rnr</name>
        <name evidence="3" type="ordered locus">NP_0874A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.13.1" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322164.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU99"/>
    <dbReference type="STRING" id="348780.NP_0874A"/>
    <dbReference type="EnsemblBacteria" id="CAI48528">
        <property type="protein sequence ID" value="CAI48528"/>
        <property type="gene ID" value="NP_0874A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700985"/>
    <dbReference type="KEGG" id="nph:NP_0874A"/>
    <dbReference type="eggNOG" id="arCOG04686">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_637142_0_0_2"/>
    <dbReference type="OrthoDB" id="134933at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008859">
        <property type="term" value="F:exoribonuclease II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001900">
        <property type="entry name" value="RNase_II/R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050180">
        <property type="entry name" value="RNR_Ribonuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355:SF9">
        <property type="entry name" value="DIS3-LIKE EXONUCLEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355">
        <property type="entry name" value="RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00773">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00955">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNB" evidence="2">
        <location>
            <begin position="72"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="434" mass="49879" checksum="669254570B78B81C" modified="2017-05-10" version="1">MTDRDEQAYAGTAEGKGPVQIDEELARHLKNKREELFEEFEIRDEFPSEVLQEAEERAEDPEGDIVDELEERRDLRDLTTWTTDPADAQDFDDAISVKRTDDGYRLWVHIADVTHYVTPETAMWDEALERANTVYLPAYTTHMLPPVLAETVCSLVPNEDRLAHTVEMHIDGETLSHESIDIYKSVIHSDARQTYNDCEDRLDDEDAPLHEENQLAYELAEKLHEQRKEDGSLVLNPKRDRAHTIIEECMLKANKAVTHTLQWDRGVEAMFRVHPQPTPDEWNEALQEIQDLDGVSIPGEAWEDPRKAVNATLEEAPRRQLDKIQWAVMKVMPRAKYMSDPFGGHHALNFEIYGHFTSPIRRLSDLVNHWIIYTNEVPEGIADLCDHASDKQKDGETCERIYKDFLEEVGLDPHAVNNRGVEVVDDPEDADHTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUA4</accession>
    <name>A0A1U7EUA4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Pyridoxal phosphate-dependent aminotransferase</fullName>
            <ecNumber evidence="7">2.6.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">NP_0884A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322169.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUA4"/>
    <dbReference type="STRING" id="348780.NP_0884A"/>
    <dbReference type="EnsemblBacteria" id="CAI48533">
        <property type="protein sequence ID" value="CAI48533"/>
        <property type="gene ID" value="NP_0884A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702495"/>
    <dbReference type="KEGG" id="nph:NP_0884A"/>
    <dbReference type="eggNOG" id="arCOG00082">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027686_1_1_2"/>
    <dbReference type="OrthoDB" id="35685at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008453">
        <property type="term" value="F:alanine-glyoxylate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004760">
        <property type="term" value="F:L-serine-pyruvate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019265">
        <property type="term" value="P:glycine biosynthetic process, by transamination of glyoxylate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024169">
        <property type="entry name" value="SP_NH2Trfase/AEP_transaminase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21152:SF40">
        <property type="entry name" value="ALANINE--GLYOXYLATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21152">
        <property type="entry name" value="AMINOTRANSFERASE CLASS V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000524">
        <property type="entry name" value="SPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="6">
        <location>
            <begin position="38"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="367" mass="41356" checksum="AF5AE75E6D919E4B" modified="2017-05-10" version="1">MSKKQEYRDDYPEKTLYLPGPTEVRDDVIKAMCEPMFGHRMDRMTDLYTTIVEDTKEFLGTDNDVIILTASGTEFWESTTLNLVEDNMLVPTSGAFSERQANVAERLGKDVDRIEYEWGKAAKPDDIRDALETGDYDAVGAVMNETSTGVRNPIEEIGDVVAEYPDTYFIVDAISCLGGDFIDIEEHGIDAIFTSTQKAFAMPPGLAVCAVSDAAYERELESESASWYGGFQRCLDYYDRKGQTHSTPAIPIMLAYRKQMKHMLDEGHGARDERHQEMAEYTREWAREHFGVFPEEGYESQTVTCVENTRNINVAETVEAVSEEYDMVFSSGYGDIGEETFRIGHMGEHTVESIKELTDAIEDVADL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUB4</accession>
    <name>A0A1U7EUB4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">S-adenosylmethionine hydroxide adenosyltransferase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_0906A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAM hydrolase / SAM-dependent halogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322180.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUB4"/>
    <dbReference type="STRING" id="348780.NP_0906A"/>
    <dbReference type="EnsemblBacteria" id="CAI48544">
        <property type="protein sequence ID" value="CAI48544"/>
        <property type="gene ID" value="NP_0906A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700996"/>
    <dbReference type="KEGG" id="nph:NP_0906A"/>
    <dbReference type="eggNOG" id="arCOG04309">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059734_1_1_2"/>
    <dbReference type="OrthoDB" id="372224at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.90">
        <property type="entry name" value="Bacterial fluorinating enzyme like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10790">
        <property type="entry name" value="S-adenosyl-l-methionine hydroxide adenosyltransferase, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046470">
        <property type="entry name" value="SAM_HAT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046469">
        <property type="entry name" value="SAM_HAT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002747">
        <property type="entry name" value="SAM_OH_AdoTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023227">
        <property type="entry name" value="SAM_OH_AdoTrfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023228">
        <property type="entry name" value="SAM_OH_AdoTrfase_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35092">
        <property type="entry name" value="CHLORINASE MJ1651"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35092:SF1">
        <property type="entry name" value="CHLORINASE MJ1651"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20257">
        <property type="entry name" value="SAM_HAT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01887">
        <property type="entry name" value="SAM_HAT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006779">
        <property type="entry name" value="UCP006779"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101852">
        <property type="entry name" value="Bacterial fluorinating enzyme, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102522">
        <property type="entry name" value="Bacterial fluorinating enzyme, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="S-adenosyl-l-methionine hydroxide adenosyltransferase N-terminal" evidence="3">
        <location>
            <begin position="2"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="S-adenosyl-l-methionine hydroxide adenosyltransferase C-terminal" evidence="4">
        <location>
            <begin position="175"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI48544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="260" mass="27028" checksum="8B4530713C3CFCFA" modified="2017-05-10" version="1">MITLSSDFGTPYPAAMKGVILKQCDARLVDVAHDFPRQDVRSTAFWLTQTLPEFPPAVHLIVVDPGVGTGRDALVVRAGDHVLVGPDNGVLFPPARALAGGDDDYSADIDAYQLDMGDPRSSTFHGRDVFAPGAAAVHETGIDRLPELDCLSPASSVVDLTLPSPEFDGDAVTAAVLAVDDFGNVITNVPGTVLGDSDRVVVDGEERPVVDSYDHAATGTAVVTVGSHGNVELAVNRGRGDDAFGVSAGETVRLDGIDAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUF6</accession>
    <name>A0A1U7EUF6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF4341/DUF395 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0984A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322218.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUF6"/>
    <dbReference type="STRING" id="348780.NP_0984A"/>
    <dbReference type="EnsemblBacteria" id="CAI48583">
        <property type="protein sequence ID" value="CAI48583"/>
        <property type="gene ID" value="NP_0984A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701020"/>
    <dbReference type="KEGG" id="nph:NP_0984A"/>
    <dbReference type="eggNOG" id="arCOG04788">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037802_2_1_2"/>
    <dbReference type="OrthoDB" id="200254at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046513">
        <property type="entry name" value="DUF6691"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20398">
        <property type="entry name" value="DUF6691"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="145" mass="15354" checksum="39B5EED835EA3767" modified="2017-05-10" version="1">MADRHPLFIPLVLAGGLVFGFGLGFSHMARPEVVLAFLTFDDLGLLFVMFGGAAVAGLTFWVLPRLLSVAPLTGMAYGRRLKSFDRNVLFGGAIFGVGWGLSGICPGAAYASLGVGNWPILYAVVGMFLGAYAQGVWRSRRAPDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUG1</accession>
    <name>A0A1U7EUG1_NATPD</name>
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        <submittedName>
            <fullName evidence="7">A-type ATP synthase subunit K</fullName>
            <ecNumber evidence="7">3.6.3.14</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">atpK</name>
        <name evidence="7" type="ordered locus">NP_1022A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.6.3.14" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322237.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUG1"/>
    <dbReference type="STRING" id="348780.NP_1022A"/>
    <dbReference type="EnsemblBacteria" id="CAI48602">
        <property type="protein sequence ID" value="CAI48602"/>
        <property type="gene ID" value="NP_1022A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702546"/>
    <dbReference type="KEGG" id="nph:NP_1022A"/>
    <dbReference type="eggNOG" id="arCOG02455">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148047_6_0_2"/>
    <dbReference type="OrthoDB" id="50539at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033177">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, proton-transporting domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.610">
        <property type="entry name" value="lithium bound rotor ring of v- atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="6">
        <location>
            <begin position="35"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="92" mass="8890" checksum="777BEDC4A677FFC7" modified="2017-05-10" version="1">MFELATVASSVAPVLQNGAEAVEAGGPALDANAVAALAVGLAALGAGYAERGIGAAAVGAMAENEDLFGKGLVLTALPETLVILALVVVFVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUG9</accession>
    <name>A0A1U7EUG9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1060A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322257.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUG9"/>
    <dbReference type="STRING" id="348780.NP_1060A"/>
    <dbReference type="EnsemblBacteria" id="CAI48621">
        <property type="protein sequence ID" value="CAI48621"/>
        <property type="gene ID" value="NP_1060A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701044"/>
    <dbReference type="KEGG" id="nph:NP_1060A"/>
    <dbReference type="HOGENOM" id="CLU_2091371_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="116" mass="11825" checksum="B72FA8D3AE5155DA" modified="2017-05-10" version="1">MRALQFALAVLLAAPAGGLALVGYLWYRHAPRLYAQWLFPDGRLHAGLVRCGVAAGCIAFGGVLLALALALGTGSVSHSGVVVSPQCRFRTAATGLLITAVAAFSAAGVGYVSGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUH8</accession>
    <name>A0A1U7EUH8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Hsp20-type molecular chaperone</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">hsp20B</name>
        <name evidence="5" type="ordered locus">NP_1078A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUH8"/>
    <dbReference type="STRING" id="348780.NP_1078A"/>
    <dbReference type="EnsemblBacteria" id="CAI48630">
        <property type="protein sequence ID" value="CAI48630"/>
        <property type="gene ID" value="NP_1078A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702830"/>
    <dbReference type="KEGG" id="nph:NP_1078A"/>
    <dbReference type="eggNOG" id="arCOG01832">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046737_8_8_2"/>
    <dbReference type="OrthoDB" id="198277at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031107">
        <property type="entry name" value="Small_HSP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11527">
        <property type="entry name" value="HEAT-SHOCK PROTEIN 20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SHSP" evidence="4">
        <location>
            <begin position="25"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="72"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="86"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI48630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="143" mass="15653" checksum="1E03A523FC038063" modified="2017-05-10" version="1">MTRNPFEELERMFDRMSSQLEPLDDELLGGSAPVDLEDRGDAYVVTVDVPGFDEGDIDVELAGETLTISATKTESNVAADEETDGDGSRYLRRERSQQSVSRSVRLPEAVDETACEATYRNGVLTVTLPKVDADTDGHSIPVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUL8</accession>
    <name>A0A1U7EUL8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ArCOG06342 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1166A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322310.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUL8"/>
    <dbReference type="EnsemblBacteria" id="CAI48674">
        <property type="protein sequence ID" value="CAI48674"/>
        <property type="gene ID" value="NP_1166A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701073"/>
    <dbReference type="KEGG" id="nph:NP_1166A"/>
    <dbReference type="eggNOG" id="arCOG06342">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111886_0_0_2"/>
    <dbReference type="OrthoDB" id="178099at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.20">
        <property type="entry name" value="Apolipoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58113">
        <property type="entry name" value="Apolipoprotein A-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="102"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="214" mass="23842" checksum="AE6F06A9D6265623" modified="2017-05-10" version="1">MSESTSPVSIVFDLQRSAIEQTHEAVENGVEVQKNLNETIVDGLDPARDLSERSSGLLRTGVDTYFDAIESAVPGSQDVVADIRDSIDEQLDTLEESQTDALDQLEENLQDGSESVDELLDDFLGTLEEQVNTLLDAHEDLEDQTVEALEELETQLQDLQDEFEERGEELQSQLEDQVDTLQEQIEDQADTLQNQVEDVTEQVQDATNPDDDSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUU2</accession>
    <name>A0A1U7EUU2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Major facilitator superfamily transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">tp15</name>
        <name evidence="10" type="ordered locus">NP_1362A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUU2"/>
    <dbReference type="STRING" id="348780.NP_1362A"/>
    <dbReference type="EnsemblBacteria" id="CAI48772">
        <property type="protein sequence ID" value="CAI48772"/>
        <property type="gene ID" value="NP_1362A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_1362A"/>
    <dbReference type="eggNOG" id="arCOG00130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_10_6_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17473">
        <property type="entry name" value="MFS_arabinose_efflux_permease_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050171">
        <property type="entry name" value="MFS_Transporters"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23517:SF3">
        <property type="entry name" value="INTEGRAL MEMBRANE TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23517">
        <property type="entry name" value="RESISTANCE PROTEIN MDTM, PUTATIVE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="142"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="172"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="270"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="306"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="332"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="366"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="396"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="17"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="199"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI48772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="421" mass="42463" checksum="62DD30EEBB5DC703" modified="2017-05-10" version="1">MPAGRNSPRMDRPGRATLWVIVASATLTVMAGAILGPVVPGIQDGLDITESQAGLVITTHAAFIVLASPLAGAAIDRFGPRRPYAFGLGIYAVGGGAGLGIDAYVPLLASRAVLGVGVAFVHTGITVLIYEYYTGQAMDRALGLRTGANSVGAAVWPLVGGALGVIAWQVPFAVYLVAAPLGVAALLVLPEPLDAASSEAAGGDASTAATSDDAPATVDDDNDNTPATASGVVGVFASRPSLLAVYLLYGAANALLYAIVVFYPQLLETLGVASALGISLYLSANGVAGGIAGASYDRLKQATGSLWLVAVAFLLWTIGFGTAAVVESPAAAFLPVVLFGIGLGLVFPSSFVWVERLAPTDRQGQFGSYVAMAGYIGQFSAPVVFGPLVAPFGVRAVFSAAALAAVGGLAAVGVALWRSNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV24</accession>
    <name>A0A1U7EV24_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF7322 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_1512A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322481.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV24"/>
    <dbReference type="STRING" id="348780.NP_1512A"/>
    <dbReference type="EnsemblBacteria" id="CAI48847">
        <property type="protein sequence ID" value="CAI48847"/>
        <property type="gene ID" value="NP_1512A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701165"/>
    <dbReference type="KEGG" id="nph:NP_1512A"/>
    <dbReference type="eggNOG" id="arCOG06289">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1998808_0_0_2"/>
    <dbReference type="OrthoDB" id="170744at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055746">
        <property type="entry name" value="DUF7322"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24008">
        <property type="entry name" value="DUF7322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="52"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7322" evidence="3">
        <location>
            <begin position="43"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="124" mass="13289" checksum="ECD1A80FB25E9FBE" modified="2017-05-10" version="1">MTSSTDDEESWLDDPGEFDPDSLGPDPPDTSSSSLQDSIQATEDVPDGLFRAFWASVLLLNVAVAALSIGAMFVYFRGDYETGGAALAIGAVAAFGTFRYYWGVKTGRYTDAAESTPQQESDNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV83</accession>
    <name>A0A1U7EV83_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6 8">Diaminopimelate decarboxylase</fullName>
            <shortName evidence="5">DAP decarboxylase</shortName>
            <shortName evidence="5">DAPDC</shortName>
            <ecNumber evidence="5 6 8">4.1.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 12" type="primary">lysA</name>
        <name evidence="12" type="ordered locus">NP_1646A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Specifically catalyzes the decarboxylation of meso-diaminopimelate (meso-DAP) to L-lysine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 8">
            <text>meso-2,6-diaminopimelate + H(+) = L-lysine + CO2</text>
            <dbReference type="Rhea" id="RHEA:15101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
            <dbReference type="EC" id="4.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 5 7 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5 8">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; L-lysine from DL-2,6-diaminopimelate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the Orn/Lys/Arg decarboxylase class-II family. LysA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.20" evidence="5 6 8"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="RefSeq" id="WP_011322548.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV83"/>
    <dbReference type="STRING" id="348780.NP_1646A"/>
    <dbReference type="EnsemblBacteria" id="CAI48914">
        <property type="protein sequence ID" value="CAI48914"/>
        <property type="gene ID" value="NP_1646A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702892"/>
    <dbReference type="KEGG" id="nph:NP_1646A"/>
    <dbReference type="eggNOG" id="arCOG02268">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026444_0_2_2"/>
    <dbReference type="OrthoDB" id="18565at2157"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00027"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008836">
        <property type="term" value="F:diaminopimelate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="CDD" id="cd06828">
        <property type="entry name" value="PLPDE_III_DapDC"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Alanine racemase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Lyase, Ornithine Decarboxylase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="LysA"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
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    <dbReference type="InterPro" id="IPR002986">
        <property type="entry name" value="DAP_deCOOHase_LysA"/>
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    <dbReference type="InterPro" id="IPR022643">
        <property type="entry name" value="De-COase2_C"/>
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        <property type="entry name" value="De-COase2_CS"/>
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    <dbReference type="InterPro" id="IPR022644">
        <property type="entry name" value="De-COase2_N"/>
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    <dbReference type="InterPro" id="IPR022653">
        <property type="entry name" value="De-COase2_pyr-phos_BS"/>
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        <property type="entry name" value="Orn/DAP/Arg_de-COase"/>
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        <property type="entry name" value="lysA"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DIAMINOPIMELATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43727:SF2">
        <property type="entry name" value="GROUP IV DECARBOXYLASE"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF02784">
        <property type="entry name" value="Orn_Arg_deC_N"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00278">
        <property type="entry name" value="Orn_DAP_Arg_deC"/>
        <property type="match status" value="1"/>
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    <dbReference type="PRINTS" id="PR01181">
        <property type="entry name" value="DAPDCRBXLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01179">
        <property type="entry name" value="ODADCRBXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50621">
        <property type="entry name" value="Alanine racemase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="PLP-binding barrel"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS00878">
        <property type="entry name" value="ODR_DC_2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00879">
        <property type="entry name" value="ODR_DC_2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 5 8" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="4 5 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 8" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="3 5 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orn/DAP/Arg decarboxylase 2 C-terminal" evidence="10">
        <location>
            <begin position="31"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="Orn/DAP/Arg decarboxylase 2 N-terminal" evidence="11">
        <location>
            <begin position="33"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="374"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="385"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="334"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="265"/>
            <end position="268"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="5 7">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
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    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="NCBIfam" id="TIGR01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600183-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI48914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="410" mass="44405" checksum="A82F3379DF6A1BFD" modified="2017-05-10" version="1">MTPPVKRLADWDGDRLERLAEAHGTPLYVMDHDRIRANYARFSEAFDFAHVMYAAKANTGRAVVRTLYDAGADIECAAAGELYRAIEAGVAPEDLQYTAVNPPAGDLDYAVDLWEDTPELTITAGAEMTLDRLEARGFDGRLALRVNPGIGTGHHEKVATGKDAKFGLPYEEVEAVAEAARERFDLVGLHAHVGSGVLSGDLDDHERALQRVADLAERVGDLEFVDLGGGFGVPYRPDEEPLDIEAAAEMVRRVTADLDATVALEPGRYLVADAGALVTRVNTIKETPETRVVGVDAGLTSMIRPAMFDSYHHIRNVTGGDREPVESSVGGPLCTSADVFCTDRPIPRPEADDLLAIGNVGAYGINLASQFHSQPRPGEVAIEGDNERVTRRRETFEDLTRLEDEASGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV87</accession>
    <name>A0A1U7EV87_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Translation initiation factor 2 subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 10">aIF2-alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 10">eIF-2-alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">tif2a</name>
        <name evidence="10" type="synonym">eif2a</name>
        <name evidence="13" type="ordered locus">NP_1618A</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 10">Heterotrimer composed of an alpha, a beta and a gamma chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the eIF-2-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322534.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV87"/>
    <dbReference type="STRING" id="348780.NP_1618A"/>
    <dbReference type="EnsemblBacteria" id="CAI48900">
        <property type="protein sequence ID" value="CAI48900"/>
        <property type="gene ID" value="NP_1618A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703265"/>
    <dbReference type="KEGG" id="nph:NP_1618A"/>
    <dbReference type="eggNOG" id="arCOG04107">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033458_0_2_2"/>
    <dbReference type="OrthoDB" id="84794at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04452">
        <property type="entry name" value="S1_IF2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.190:FF:000006">
        <property type="entry name" value="Translation initiation factor 2 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1130:FF:000002">
        <property type="entry name" value="Translation initiation factor 2 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1130">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.190">
        <property type="entry name" value="Translation initiation factor 2, subunit 1, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00231">
        <property type="entry name" value="eIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044126">
        <property type="entry name" value="S1_IF2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022964">
        <property type="entry name" value="TIF2_asu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024055">
        <property type="entry name" value="TIF2_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024054">
        <property type="entry name" value="TIF2_asu_middle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011488">
        <property type="entry name" value="TIF_2_asu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003064">
        <property type="entry name" value="PRK03987.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003065">
        <property type="entry name" value="PRK03987.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07541">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110993">
        <property type="entry name" value="eIF-2-alpha, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116742">
        <property type="entry name" value="eIF2alpha middle domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10 13" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="S1 motif" evidence="12">
        <location>
            <begin position="10"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="236"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="256"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAI48900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="266" mass="29571" checksum="414FD65FD7B33F75" modified="2017-05-10" version="1">MKYSGWPTPGELVVGRVDEIEDFGVFVDLEEYEEKRGLVHVSEVASGWIKNVRDHVNEDQMVVCKVLEVDESAQQVDLSIKDVNDHQRSEKVQEWKNEQKADKWMELAFGEDVDDGTFRRIANELIDAHGSLYAGFEQAAIHGPEALDATDLDDDELDAIVETARENVSVPYVTVTGYVDLEAPGGDGVEDVREALQAAEGNGEVPEEVDLDVTYVGAPEYRIRVQAPNYKTAESELEASAERASDAIEAVGGTAEFHRERRTDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVA8</accession>
    <name>A0A1U7EVA8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF1486 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1698A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322573.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVA8"/>
    <dbReference type="STRING" id="348780.NP_1698A"/>
    <dbReference type="EnsemblBacteria" id="CAI48940">
        <property type="protein sequence ID" value="CAI48940"/>
        <property type="gene ID" value="NP_1698A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701228"/>
    <dbReference type="KEGG" id="nph:NP_1698A"/>
    <dbReference type="eggNOG" id="arCOG03106">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147287_1_1_2"/>
    <dbReference type="OrthoDB" id="145984at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037401">
        <property type="entry name" value="SnoaL-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12680">
        <property type="entry name" value="SnoaL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SnoaL-like" evidence="1">
        <location>
            <begin position="7"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="112" mass="12517" checksum="E361EFF9CB451259" modified="2017-05-10" version="1">MERPEAVRAYYRAIDEDDYEALSDLLAGGFVHRRPDTVIEGREEFVAFMRGGRPERDTSHELSAVYGDSGRIAAEGTLYRSDGTAWFGFVDTFGFVASGISEIRTYTDAHPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVF8</accession>
    <name>A0A1U7EVF8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UspA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_1826A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322636.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVF8"/>
    <dbReference type="STRING" id="348780.NP_1826A"/>
    <dbReference type="EnsemblBacteria" id="CAI49004">
        <property type="protein sequence ID" value="CAI49004"/>
        <property type="gene ID" value="NP_1826A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701266"/>
    <dbReference type="KEGG" id="nph:NP_1826A"/>
    <dbReference type="eggNOG" id="arCOG02053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_11_0_2"/>
    <dbReference type="OrthoDB" id="105697at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF6">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN UP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="149" mass="15785" checksum="22DD3D40DD238B7E" modified="2017-05-10" version="1">MFETVLMPTDGSKPAEGAVETGAELAAEHGATVHALYAVEPMPLGKFTAGPEPASAEHGEILDEQKSEAKQALERVTELCADHGVDVVDTIEYGKPSEEILDYVDTEGMDAVVMGTHGRSGAERLVMGSVAEKVVRKSPVPVLTIRPDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVK0</accession>
    <name>A0A1U7EVK0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphatase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1918A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322682.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVK0"/>
    <dbReference type="STRING" id="348780.NP_1918A"/>
    <dbReference type="EnsemblBacteria" id="CAI49050">
        <property type="protein sequence ID" value="CAI49050"/>
        <property type="gene ID" value="NP_1918A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701303"/>
    <dbReference type="KEGG" id="nph:NP_1918A"/>
    <dbReference type="eggNOG" id="arCOG01153">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_728852_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051918">
        <property type="entry name" value="STPP_CPPED1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43143">
        <property type="entry name" value="METALLOPHOSPHOESTERASE, CALCINEURIN SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43143:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE CPPED1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="1">
        <location>
            <begin position="5"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="379" mass="42909" checksum="3F6F1F36DEC3F0EB" modified="2017-05-10" version="1">MPIECPYCSEYFDSKESFAVHLVEEHEWEQLSRIDKKRVRTFCPEKTPDRGPLSRLSLRIKNSSAGRRVSSTDVRNRITRRGLLRAGAVSTVGLGGIYGLNWYLQQEPTLLQENPDLIETRQRGIDGSIINGTAFNSIYDRQSVSMADVKYRFVVVSDSHWGHNYQGDYGPQGWVEAIERAKDEIEAVHDEREIDLLFHNGDIIHNDPEDHFTVIEEFFSELPDPIEWYPVMGNHDLLPGEEWEAVYDIPKDYTVELGEYVFIICDTAEPGDPGGRPGADVDFLRSQIDAFADKAGVFVLMHIPQTSAEGGSGDPDAVRAQLARDPVTATFFAHRHGKNEVQQLNSNNDRTVCYCPLIGDERPHVQRGFRVVDVIETTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVM8</accession>
    <name>A0A1U7EVM8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2006A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49094.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322723.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVM8"/>
    <dbReference type="STRING" id="348780.NP_2006A"/>
    <dbReference type="EnsemblBacteria" id="CAI49094">
        <property type="protein sequence ID" value="CAI49094"/>
        <property type="gene ID" value="NP_2006A"/>
    </dbReference>
    <dbReference type="GeneID" id="54763313"/>
    <dbReference type="KEGG" id="nph:NP_2006A"/>
    <dbReference type="eggNOG" id="arCOG08910">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_214143_0_0_2"/>
    <dbReference type="OrthoDB" id="154701at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49094.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="52" mass="5512" checksum="796E85CBCE5E351A" modified="2017-05-10" version="1">MFDILLHAGTEHPNLSFIVVASLLSFLAGLGIGTYSEQLAGLFTSTDSEPTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVP4</accession>
    <name>A0A1U7EVP4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Aconitate hydratase</fullName>
            <ecNumber evidence="9">4.2.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">citB2</name>
        <name evidence="9" type="ordered locus">NP_1994A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.3" evidence="9"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322717.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVP4"/>
    <dbReference type="STRING" id="348780.NP_1994A"/>
    <dbReference type="EnsemblBacteria" id="CAI49088">
        <property type="protein sequence ID" value="CAI49088"/>
        <property type="gene ID" value="NP_1994A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702610"/>
    <dbReference type="KEGG" id="nph:NP_1994A"/>
    <dbReference type="eggNOG" id="arCOG01697">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013476_2_1_2"/>
    <dbReference type="OrthoDB" id="255at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003994">
        <property type="term" value="F:aconitate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01580">
        <property type="entry name" value="AcnA_IRP_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.19.10:FF:000001">
        <property type="entry name" value="Aconitate hydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044137">
        <property type="entry name" value="AcnA_IRP_Swivel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006249">
        <property type="entry name" value="Aconitase/IRP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01341">
        <property type="entry name" value="aconitase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006757">
        <property type="entry name" value="PRK09277.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009520">
        <property type="entry name" value="PRK12881.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11670">
        <property type="entry name" value="ACONITASE/IRON-RESPONSIVE ELEMENT FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="7">
        <location>
            <begin position="79"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="8">
        <location>
            <begin position="713"/>
            <end position="839"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI49088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="917" mass="99902" checksum="E12200298997F6DB" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVQ3</accession>
    <name>A0A1U7EVQ3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glutaredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">grx4</name>
        <name evidence="4" type="ordered locus">NP_2066A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322753.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVQ3"/>
    <dbReference type="SMR" id="A0A1U7EVQ3"/>
    <dbReference type="STRING" id="348780.NP_2066A"/>
    <dbReference type="EnsemblBacteria" id="CAI49124">
        <property type="protein sequence ID" value="CAI49124"/>
        <property type="gene ID" value="NP_2066A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701340"/>
    <dbReference type="KEGG" id="nph:NP_2066A"/>
    <dbReference type="eggNOG" id="arCOG04648">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026126_2_1_2"/>
    <dbReference type="OrthoDB" id="203033at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004480">
        <property type="entry name" value="Monothiol_GRX-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293">
        <property type="entry name" value="GLUTAREDOXIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293:SF16">
        <property type="entry name" value="GLUTAREDOXIN-RELATED PROTEIN 5, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="3">
        <location>
            <begin position="28"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="135" mass="14783" checksum="197BA5E65B698A7F" modified="2017-05-10" version="1">MTFDPNADAADPENVQERVDTLIEDNEVVLFMKGNELMPQCGYSKKALQLLTKYRDDVVTEDVLDALDAYRAALESHSGWETIPQTFVDGEFIGGSDILEELDNRGELHDELGVDADDVDADDAGTDDAGAEAPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVT5</accession>
    <name>A0A1U7EVT5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-glutamate O-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.80</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">cheR</name>
        <name evidence="7" type="ordered locus">NP_2170A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamyl-[protein] + S-adenosyl-L-methionine = [protein]-L-glutamate 5-O-methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:24452"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:10311"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:82795"/>
            <dbReference type="EC" id="2.1.1.80"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.80" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322804.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVT5"/>
    <dbReference type="STRING" id="348780.NP_2170A"/>
    <dbReference type="EnsemblBacteria" id="CAI49176">
        <property type="protein sequence ID" value="CAI49176"/>
        <property type="gene ID" value="NP_2170A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702602"/>
    <dbReference type="KEGG" id="nph:NP_2170A"/>
    <dbReference type="eggNOG" id="arCOG04402">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025854_1_1_2"/>
    <dbReference type="OrthoDB" id="10657at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008983">
        <property type="term" value="F:protein-glutamate O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.155.10">
        <property type="entry name" value="Chemotaxis receptor methyltransferase CheR, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050903">
        <property type="entry name" value="Bact_Chemotaxis_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022642">
        <property type="entry name" value="CheR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000780">
        <property type="entry name" value="CheR_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022641">
        <property type="entry name" value="CheR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036804">
        <property type="entry name" value="CheR_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24422">
        <property type="entry name" value="CHEMOTAXIS PROTEIN METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24422:SF10">
        <property type="entry name" value="CHEMOTAXIS PROTEIN METHYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01739">
        <property type="entry name" value="CheR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03705">
        <property type="entry name" value="CheR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00996">
        <property type="entry name" value="CHERMTFRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00138">
        <property type="entry name" value="MeTrc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47757">
        <property type="entry name" value="Chemotaxis receptor methyltransferase CheR, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50123">
        <property type="entry name" value="CHER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CheR-type methyltransferase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="272" mass="31042" checksum="1752151B153D6F84" modified="2017-05-10" version="1">MKGTDTDTGFEAVVRYVADNMAFEPDSYNESYLDRRISARMRRTDCDDYEGYLDILADSEDEQEALLDALSINVTSFFRNPPVWERLRDVLAMLTEEHRTVNCWSAPCADGREPYSIAMLARDDPNIRERAVEITATDIDAEALAEAREGVYESTRTTDIGSQLEPLSDPEAHVEITDDGTSFAVKDDVKRMVTFEKHDLISGRPKSGHDLVLCRNLLIYIDKSYKEPIFETLTDALREGGFLTIGKSETLPRSFRDAYDPYDRGKHIYRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVY2</accession>
    <name>A0A1U7EVY2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2248A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322842.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVY2"/>
    <dbReference type="STRING" id="348780.NP_2248A"/>
    <dbReference type="EnsemblBacteria" id="CAI49215">
        <property type="protein sequence ID" value="CAI49215"/>
        <property type="gene ID" value="NP_2248A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701391"/>
    <dbReference type="KEGG" id="nph:NP_2248A"/>
    <dbReference type="eggNOG" id="arCOG03104">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069299_0_0_2"/>
    <dbReference type="OrthoDB" id="211869at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043901">
        <property type="entry name" value="DUF5787"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19100">
        <property type="entry name" value="DUF5787"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="275"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="275"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="293"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="319" mass="34873" checksum="D739AE6B8EA2AB1F" modified="2017-05-10" version="1">MREFAFELAVAASLEAATDGLVARQLGTRDRIVDLVEIEPGPDFSERTSLTAEPIPPLAIESAVGVGTARDPVEAIDTDPDHARSVADQAIEAGFFTAERRGSRRHVRQTARYPDWFAGLHAYENKPDLDRPGDLERQLRKDVSLALFDTVTLVTDSYVTGAHRNRIPEVVGIVRFDAEHGETTVIRDAEPLSTETTGIAIGERSPARVDIEPIPSERKTALRLRLAERAYGKGWRPDGLPPCTQADARGPPFASDDALPYCQWKGRFVHPTRECGPDCGGHEAAEPPPADIDAVRAEKTPWEPDPPGAGRTQTSLDDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW01</accession>
    <name>A0A1U7EW01_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">NADH dehydrogenase-like complex subunit K</fullName>
            <ecNumber evidence="8">1.6.5.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">nuoK</name>
        <name evidence="8" type="ordered locus">NP_2308A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I subunit 4L family.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.-" evidence="8"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322872.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW01"/>
    <dbReference type="STRING" id="348780.NP_2308A"/>
    <dbReference type="EnsemblBacteria" id="CAI49245">
        <property type="protein sequence ID" value="CAI49245"/>
        <property type="gene ID" value="NP_2308A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702996"/>
    <dbReference type="KEGG" id="nph:NP_2308A"/>
    <dbReference type="eggNOG" id="arCOG03073">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144724_0_0_2"/>
    <dbReference type="OrthoDB" id="56168at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030964">
        <property type="term" value="C:NADH dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.3510:FF:000001">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01456">
        <property type="entry name" value="NDH1_NuoK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001133">
        <property type="entry name" value="NADH_UbQ_OxRdtase_chain4L/K"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039428">
        <property type="entry name" value="NUOK/Mnh_C1-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004320">
        <property type="entry name" value="PRK05715.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004321">
        <property type="entry name" value="PRK05715.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004323">
        <property type="entry name" value="PRK05715.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11434:SF16">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 4L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11434">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE SUBUNIT ND4L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00420">
        <property type="entry name" value="Oxidored_q2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="104" mass="11444" checksum="2B90F51CEEDBC6F6" modified="2017-05-10" version="1">MVLETVPAEYYLVLSAAVFCIGLFGILTRRNALMFLMSVELLLNAANINLVVFAFYWGEVTGQVFVLFTMALAAAEVAIGIGIILVLHRNFDSVDVTEAATLRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW14</accession>
    <name>A0A1U7EW14_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">NADH dehydrogenase-like complex subunit CD</fullName>
            <ecNumber evidence="12">1.6.5.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">nuoCD</name>
        <name evidence="12" type="ordered locus">NP_2296A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a quinone + NADH + 5 H(+)(in) = a quinol + NAD(+) + 4 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">NDH-1 is composed of 13 different subunits. Subunits NuoB, CD, E, F, and G constitute the peripheral sector of the complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the complex I 49 kDa subunit family.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.-" evidence="12"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322866.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW14"/>
    <dbReference type="STRING" id="348780.NP_2296A"/>
    <dbReference type="EnsemblBacteria" id="CAI49239">
        <property type="protein sequence ID" value="CAI49239"/>
        <property type="gene ID" value="NP_2296A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702991"/>
    <dbReference type="KEGG" id="nph:NP_2296A"/>
    <dbReference type="eggNOG" id="arCOG01548">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015134_3_2_2"/>
    <dbReference type="OrthoDB" id="43567at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="entry name" value="Cytochrome-c3 Hydrogenase, chain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.80">
        <property type="entry name" value="NADH:ubiquinone oxidoreductase, 30kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01358">
        <property type="entry name" value="NDH1_NuoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001135">
        <property type="entry name" value="NADH_Q_OxRdtase_suD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037232">
        <property type="entry name" value="NADH_quin_OxRdtase_su_C/D-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001268">
        <property type="entry name" value="NADH_UbQ_OxRdtase_30kDa_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020396">
        <property type="entry name" value="NADH_UbQ_OxRdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022885">
        <property type="entry name" value="NDH1_su_D/H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004739">
        <property type="entry name" value="PRK06075.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11993:SF10">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11993">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 49 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00329">
        <property type="entry name" value="Complex1_30kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00346">
        <property type="entry name" value="Complex1_49kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="HydB/Nqo4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143243">
        <property type="entry name" value="Nqo5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00542">
        <property type="entry name" value="COMPLEX1_30K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase 30kDa subunit" evidence="10">
        <location>
            <begin position="44"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="NADH-quinone oxidoreductase subunit D" evidence="11">
        <location>
            <begin position="306"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI49239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="556" mass="63333" checksum="322B1AE56CC423DE" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW54</accession>
    <name>A0A1U7EW54_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">cytochrome-c oxidase</fullName>
            <ecNumber evidence="3">7.1.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Cytochrome c oxidase polypeptide II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">coxB1</name>
        <name evidence="17" type="ordered locus">NP_2448A</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome c oxidase subunit 2 family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.9" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322941.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW54"/>
    <dbReference type="STRING" id="348780.NP_2448A"/>
    <dbReference type="EnsemblBacteria" id="CAI49315">
        <property type="protein sequence ID" value="CAI49315"/>
        <property type="gene ID" value="NP_2448A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702626"/>
    <dbReference type="KEGG" id="nph:NP_2448A"/>
    <dbReference type="eggNOG" id="arCOG01235">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036876_4_3_2"/>
    <dbReference type="OrthoDB" id="27522at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13914">
        <property type="entry name" value="CuRO_HCO_II_like_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045187">
        <property type="entry name" value="CcO_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002429">
        <property type="entry name" value="CcO_II-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014222">
        <property type="entry name" value="Cyt_c_oxidase_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011759">
        <property type="entry name" value="Cyt_c_oxidase_su2_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036257">
        <property type="entry name" value="Cyt_c_oxidase_su2_TM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02866">
        <property type="entry name" value="CoxB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22888:SF9">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22888">
        <property type="entry name" value="CYTOCHROME C OXIDASE, SUBUNIT II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00116">
        <property type="entry name" value="COX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02790">
        <property type="entry name" value="COX2_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01166">
        <property type="entry name" value="CYCOXIDASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81464">
        <property type="entry name" value="Cytochrome c oxidase subunit II-like, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50857">
        <property type="entry name" value="COX2_CUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50999">
        <property type="entry name" value="COX2_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0186">Copper</keyword>
    <keyword evidence="9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="8" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome oxidase subunit II transmembrane region profile" evidence="16">
        <location>
            <begin position="12"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome oxidase subunit II copper A binding" evidence="15">
        <location>
            <begin position="135"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAI49315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="250" mass="28242" checksum="AC8ECEDFA30E0F83" modified="2017-05-10" version="1">MRVTRIAPVALVGALALLAVEPVAAQSTIQDELITDLNTTLLYAAIPIAILVEAILIYAVVKFRNNDDPKPTEENRRLEITWTIATAIVLLFVGFASYQVLAVEEVGNPLEDEDRIEPSVSEDLEGAVGPYEHEEDAVEIELEAYRYGWEASYADGEVSEFDQIRIPTDRPVYIHIYASDWLHMLHVPELNLKQSAFVGEYNTIKTEAYEEGSHQFYCTEYCGTGHSQMNGEFTVMDDDEFDDWLDEQLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW57</accession>
    <name>A0A1U7EW57_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NurA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2434A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322934.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW57"/>
    <dbReference type="STRING" id="348780.NP_2434A"/>
    <dbReference type="EnsemblBacteria" id="CAI49308">
        <property type="protein sequence ID" value="CAI49308"/>
        <property type="gene ID" value="NP_2434A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701443"/>
    <dbReference type="KEGG" id="nph:NP_2434A"/>
    <dbReference type="eggNOG" id="arCOG00367">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054695_0_0_2"/>
    <dbReference type="OrthoDB" id="190207at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018977">
        <property type="entry name" value="NurA_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09376">
        <property type="entry name" value="NurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00933">
        <property type="entry name" value="NurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NurA" evidence="1">
        <location>
            <begin position="74"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="420" mass="47175" checksum="29CD02C57EEFA19C" modified="2017-05-10" version="1">MTLDPVHFDGIAGLADRISHRVDATDHDAFAETVFEEFLDPLWHDGDRIVEPLGELRRRRIDIEDAALQDAPFPTQHGLDSGTINPTTFTNGLVIDIAHAAMAAVPSELSLHRSRTVVKTVHSNDRTVDCNEDDWSGFDRDGPDGDYFSRGRVLQAPHVNRYEEGVVHALSLYLAEVEHALDNAEAVEDLLVLDGPIYPKGLLAWSDRSPELADLLVENERPRDVVSSYLRLVEQFADRGVPLVGVVKNSASKAITRAVRNEKRAPWVNDAAFFERVLRRTGDDGELRTDALTFTNWFVSRCGVDEPLSKHGDALGLERNRPPEDYEVTFCCVFDPRTELLFRVEAPAVVTRDPELREALTMQLLRDIAHERGPPLAVAKADELARISRDEKRALVKTIEERFDAQEATDYNDERWTGPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWD0</accession>
    <name>A0A1U7EWD0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Mechanosensitive channel protein MscS</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">mscS3</name>
        <name evidence="11" type="ordered locus">NP_2598A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323015.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWD0"/>
    <dbReference type="STRING" id="348780.NP_2598A"/>
    <dbReference type="EnsemblBacteria" id="CAI49390">
        <property type="protein sequence ID" value="CAI49390"/>
        <property type="gene ID" value="NP_2598A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702940"/>
    <dbReference type="KEGG" id="nph:NP_2598A"/>
    <dbReference type="eggNOG" id="arCOG01568">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037945_2_0_2"/>
    <dbReference type="OrthoDB" id="11475at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049278">
        <property type="entry name" value="MS_channel_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045275">
        <property type="entry name" value="MscS_archaea/bacteria_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_beta-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011066">
        <property type="entry name" value="MscS_channel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011014">
        <property type="entry name" value="MscS_channel_TM-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30221">
        <property type="entry name" value="SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30221:SF20">
        <property type="entry name" value="SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21082">
        <property type="entry name" value="MS_channel_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82689">
        <property type="entry name" value="Mechanosensitive channel protein MscS (YggB), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82861">
        <property type="entry name" value="Mechanosensitive channel protein MscS (YggB), transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="67"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="95"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS" evidence="9">
        <location>
            <begin position="112"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS C-terminal" evidence="10">
        <location>
            <begin position="188"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="303"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="307"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI49390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="344" mass="38001" checksum="608CB0A4C144E09F" modified="2017-05-10" version="1">MDPTTTALQQGLPRPGWLPETIPLWAYRLVMAVVFLTVAYYLSQFARRLLARRIARRFERPSVGQTVLRSIQAVIVFVAVMTALGFFGIGLGNVVLSVGVFSAVAGIILAPIIGNLINGVFILSEQPYEIGDMIELEDTDTKGFVEDITLFYTKVFTLDNTFIVLPNGSMRERDVINYSAEDARVRLTADVGVTYESDIETAREQLRAAARDVDGVIKGGPDIRIGSARYPAAPVVHILEFGDSEVALRLRFWVREPYTQATVRSAVLTNVWERFDDHDIEIPYPHSHLVFDDTSGELQTAIRRRGQQAAARQGGATTRNEEQEAPASEPGTETEPTDDAGGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWD3</accession>
    <name>A0A1U7EWD3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2592A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323012.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWD3"/>
    <dbReference type="STRING" id="348780.NP_2592A"/>
    <dbReference type="EnsemblBacteria" id="CAI49387">
        <property type="protein sequence ID" value="CAI49387"/>
        <property type="gene ID" value="NP_2592A"/>
    </dbReference>
    <dbReference type="GeneID" id="43500089"/>
    <dbReference type="KEGG" id="nph:NP_2592A"/>
    <dbReference type="HOGENOM" id="CLU_3039158_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="54" mass="6281" checksum="E47EFB4558011721" modified="2017-05-10" version="1">MIRAVFLGGLLLLALVGVILLYRVTLAKLETKQTLEQRKLEHEERKELFDDEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWP6</accession>
    <name>A0A1U7EWP6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">SirR/DtxR family transcription regulator SirR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">sirR1</name>
        <name evidence="9" type="ordered locus">NP_2878A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DtxR/MntR family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323155.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWP6"/>
    <dbReference type="STRING" id="348780.NP_2878A"/>
    <dbReference type="EnsemblBacteria" id="CAI49530">
        <property type="protein sequence ID" value="CAI49530"/>
        <property type="gene ID" value="NP_2878A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701580"/>
    <dbReference type="KEGG" id="nph:NP_2878A"/>
    <dbReference type="eggNOG" id="arCOG02099">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069532_0_2_2"/>
    <dbReference type="OrthoDB" id="24735at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.10">
        <property type="entry name" value="Iron dependent repressor, metal binding and dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050536">
        <property type="entry name" value="DtxR_MntR_Metal-Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007167">
        <property type="entry name" value="Fe-transptr_FeoA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001367">
        <property type="entry name" value="Fe_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036421">
        <property type="entry name" value="Fe_dep_repressor_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038157">
        <property type="entry name" value="FeoA_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022687">
        <property type="entry name" value="HTH_DTXR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022689">
        <property type="entry name" value="Iron_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008988">
        <property type="entry name" value="Transcriptional_repressor_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33238">
        <property type="entry name" value="IRON (METAL) DEPENDENT REPRESSOR, DTXR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33238:SF7">
        <property type="entry name" value="IRON-DEPENDENT TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02742">
        <property type="entry name" value="Fe_dep_repr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01325">
        <property type="entry name" value="Fe_dep_repress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04023">
        <property type="entry name" value="FeoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00899">
        <property type="entry name" value="FeoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00529">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50037">
        <property type="entry name" value="C-terminal domain of transcriptional repressors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47979">
        <property type="entry name" value="Iron-dependent repressor protein, dimerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50944">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH dtxR-type" evidence="8">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI49530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="219" mass="24535" checksum="58F69B70DE389C4D" modified="2017-05-10" version="1">MLSAVMEDYIKAIYALQNDTDERVGTSELADYMDVTSPTVSSMIKKLEERGLVDREEYRGVRLTEEGEVVALEILRHHRLLEAFLTEHLDYDWADVHEEADRLEHHVSEELTERIAEALDNPGVDPHGDPIPDADLELPEAGETTRLTAAAEGDTVVVRRIRHQGDEELRYLAAAGIEPDVEIEVVEIAPFGLVTVQTPEGEQSLPEEIARLIEIDSDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWS3</accession>
    <name>A0A1U7EWS3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable secreted glycoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2930A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49556.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939561.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWS3"/>
    <dbReference type="STRING" id="348780.NP_2930A"/>
    <dbReference type="EnsemblBacteria" id="CAI49556">
        <property type="protein sequence ID" value="CAI49556"/>
        <property type="gene ID" value="NP_2930A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701601"/>
    <dbReference type="KEGG" id="nph:NP_2930A"/>
    <dbReference type="eggNOG" id="arCOG06227">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_793703_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49556.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="356" mass="38354" checksum="E64041DBD37BA426" modified="2017-05-10" version="1">MSSHASTRRRLLVALGSGIAVGAAGCSEAEELLDDAPNDEPDPENGDTPTDVDGEDTPDPDPENGDTPDDTEDPETPEEPDLQTREEELPELIRETIQSSRGYIQEAISVYAAGGEFDSTEPPLLSVPLVDPVDRIDVQRELDDADARLESAVSRADRQNVDDEPFQRLVELLEAEISFLFVAAETHAALADGFELVEETVDLADQNAYGLADDRHDELGELLAATTADAEVAAARADPIVAATDEEREALESPHHPELASRLEAGAEALSEYHDTFESFAETLSTLDDAATASDDDEALALSQIAIDDLETVESELEAVDIPAGVEPLHTDLEGYINERLEEAREIRDEAAAALD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWU4</accession>
    <name>A0A1U7EWU4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2926A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323178.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWU4"/>
    <dbReference type="STRING" id="348780.NP_2926A"/>
    <dbReference type="DNASU" id="3701600"/>
    <dbReference type="EnsemblBacteria" id="CAI49554">
        <property type="protein sequence ID" value="CAI49554"/>
        <property type="gene ID" value="NP_2926A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701600"/>
    <dbReference type="KEGG" id="nph:NP_2926A"/>
    <dbReference type="eggNOG" id="arCOG10197">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1202595_0_0_2"/>
    <dbReference type="OrthoDB" id="201179at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="227" mass="23653" checksum="27A4DFDDD497A3D1" modified="2017-05-10" version="1">MDGLTARRQRTAHPKFRRVAAPAAALVGVNILLMYLVAATPLAVANDLLFQVPILGLLVYGAALTGGSILAERGLESGSMELAAVGVVLLQVAYALFGGGIIAGLGGGTRGVALGIAFVVTLAMTIGIAAYVYRRGGSYDHWGRWALGAFILGALLVAVGSAIPAVLLGGFVAIFAGFTLRLGYEIWQVSANYAPDKSLRHAVGIYVAFTGVFVHVLQIVARQFLQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWW0</accession>
    <name>A0A1U7EWW0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC-type transport system periplasmic substrate-binding protein (Probable substrate dipeptide/oligopeptide)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">dppA3</name>
        <name evidence="2" type="ordered locus">NP_2986A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323208.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWW0"/>
    <dbReference type="STRING" id="348780.NP_2986A"/>
    <dbReference type="EnsemblBacteria" id="CAI49584">
        <property type="protein sequence ID" value="CAI49584"/>
        <property type="gene ID" value="NP_2986A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701616"/>
    <dbReference type="KEGG" id="nph:NP_2986A"/>
    <dbReference type="eggNOG" id="arCOG01534">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035683_0_0_2"/>
    <dbReference type="OrthoDB" id="233597at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904680">
        <property type="term" value="F:peptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.105.10">
        <property type="entry name" value="Dipeptide-binding Protein, Domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PERIPLASMIC BINDING COMPONENT OF ABC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Solute-binding protein family 5" evidence="1">
        <location>
            <begin position="254"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="587" mass="64788" checksum="6925D3B84C5BF22A" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWW1</accession>
    <name>A0A1U7EWW1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Amphi-Trp domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3000A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49591.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939566.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWW1"/>
    <dbReference type="STRING" id="348780.NP_3000A"/>
    <dbReference type="EnsemblBacteria" id="CAI49591">
        <property type="protein sequence ID" value="CAI49591"/>
        <property type="gene ID" value="NP_3000A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701622"/>
    <dbReference type="KEGG" id="nph:NP_3000A"/>
    <dbReference type="eggNOG" id="arCOG04789">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_160437_0_0_2"/>
    <dbReference type="OrthoDB" id="194858at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027598">
        <property type="entry name" value="Amphi-Trp_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04354">
        <property type="entry name" value="amphi-Trp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20068">
        <property type="entry name" value="Amphi-Trp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amphi-Trp" evidence="1">
        <location>
            <begin position="2"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49591.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="90" mass="9941" checksum="1C51B99B6DD59C41" modified="2017-05-10" version="1">MEEVLFKTEKRQSRADIASMLRTVADKLDDGGDLTLSAGDESVTLDVPSQPTFEIKAERETEGSETELSVEFEIEWDENGDSTDGDIEIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWW4</accession>
    <name>A0A1U7EWW4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ba3-type terminal oxidase subunit CbaD</fullName>
            <ecNumber evidence="2">1.9.3.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cbaD</name>
        <name evidence="2" type="ordered locus">NP_2966A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.9.3.-" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323198.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWW4"/>
    <dbReference type="STRING" id="348780.NP_2966A"/>
    <dbReference type="EnsemblBacteria" id="CAI49574">
        <property type="protein sequence ID" value="CAI49574"/>
        <property type="gene ID" value="NP_2966A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702564"/>
    <dbReference type="KEGG" id="nph:NP_2966A"/>
    <dbReference type="eggNOG" id="arCOG08132">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_199470_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="54" mass="6081" checksum="0C7511A8A14F6922" modified="2017-05-10" version="1">MGTDVEREIGHDEYDPKGTLALIAIYFLLIAGLWIFTYFVEFLGNEMTVVGVVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWY7</accession>
    <name>A0A1U7EWY7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">AAA-type ATPase (CDC48 subfamily)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cdc48a</name>
        <name evidence="9" type="ordered locus">NP_3072A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family. CDC48 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323249.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWY7"/>
    <dbReference type="STRING" id="348780.NP_3072A"/>
    <dbReference type="EnsemblBacteria" id="CAI49627">
        <property type="protein sequence ID" value="CAI49627"/>
        <property type="gene ID" value="NP_3072A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701646"/>
    <dbReference type="KEGG" id="nph:NP_3072A"/>
    <dbReference type="eggNOG" id="arCOG01308">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_12_2_2"/>
    <dbReference type="OrthoDB" id="77269at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19529">
        <property type="entry name" value="RecA-like_VCP_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000007">
        <property type="entry name" value="AAA family ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.330.10:FF:000005">
        <property type="entry name" value="AAA family ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000057">
        <property type="entry name" value="AAA family ATPase, CDC48 subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000018">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000038">
        <property type="entry name" value="spermatogenesis-associated protein 5-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000012">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005938">
        <property type="entry name" value="AAA_ATPase_CDC48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004201">
        <property type="entry name" value="Cdc48_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01243">
        <property type="entry name" value="CDC48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF201">
        <property type="entry name" value="PROTEIN CDCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02933">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02359">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01072">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="Cdc48 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CDC48 N-terminal subdomain" evidence="8">
        <location>
            <begin position="5"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="CDC48" evidence="7">
        <location>
            <begin position="106"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="222"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="495"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="721"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="721"/>
            <end position="731"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI49627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="740" mass="81730" checksum="F7D48226FC4A7F0D" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWZ2</accession>
    <name>A0A1U7EWZ2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3086A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323256.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWZ2"/>
    <dbReference type="STRING" id="348780.NP_3086A"/>
    <dbReference type="EnsemblBacteria" id="CAI49634">
        <property type="protein sequence ID" value="CAI49634"/>
        <property type="gene ID" value="NP_3086A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701648"/>
    <dbReference type="KEGG" id="nph:NP_3086A"/>
    <dbReference type="eggNOG" id="arCOG04551">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1131641_0_0_2"/>
    <dbReference type="OrthoDB" id="168607at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018641">
        <property type="entry name" value="Trfase_1_rSAM/seldom-assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36529:SF1">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36529">
        <property type="entry name" value="SLL1095 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="244" mass="25801" checksum="0127B7F290AF8758" modified="2017-05-10" version="1">MTTVAVLCDPPQPGAVLPELVEGTPVSPEDAADLYTALLCDTVEAVDGSGGELLVNYRPAEAVGVDGDAEAIIREAIADVVDTDDARFEVQVGETFAGRVGNTATHLLETEELSSVGIIRPEVATFARTDIDSAAMKLRSCDVVIGPSTGGRVHYAAFGEPIDFTDCYAAPAVETLTNRCLDAGHDVEFLETKQSVRTPQELADAIVSVRAHDTAGELVPRHFAAWIDGMPYEVVTDDDGLSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWZ9</accession>
    <name>A0A1U7EWZ9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Adenylate cyclase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3092A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323259.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWZ9"/>
    <dbReference type="STRING" id="348780.NP_3092A"/>
    <dbReference type="EnsemblBacteria" id="CAI49637">
        <property type="protein sequence ID" value="CAI49637"/>
        <property type="gene ID" value="NP_3092A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702647"/>
    <dbReference type="KEGG" id="nph:NP_3092A"/>
    <dbReference type="eggNOG" id="arCOG01723">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105244_2_0_2"/>
    <dbReference type="OrthoDB" id="46040at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07890">
        <property type="entry name" value="CYTH-like_AC_IV-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.320.10">
        <property type="entry name" value="Hypothetical Protein Pfu-838710-001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008173">
        <property type="entry name" value="Adenylyl_cyclase_CyaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033469">
        <property type="entry name" value="CYTH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023577">
        <property type="entry name" value="CYTH_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00318">
        <property type="entry name" value="cyaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21028:SF2">
        <property type="entry name" value="CYTH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21028">
        <property type="entry name" value="SI:CH211-156B7.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01928">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01118">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55154">
        <property type="entry name" value="CYTH-like phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51707">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CYTH" evidence="1">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="179" mass="19787" checksum="8608C89F5393435E" modified="2017-05-10" version="1">MYEVELKVQTDHEPVRSRLSELEAEPLGAVTQVDTYYDHPVREFGETDEALRIRRERQDGDAEARVTYKGPLVEAASKTRRELETAVEDGETMAGVLEAVGFEPAATVEKTRERFEYDGYTVTLDSVTGLGEFVEVETEAGEIEPAREGAVAVLTALGLDADEQVRTSYLGLLLDEDGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX29</accession>
    <name>A0A1U7EX29_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UspA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3146A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323286.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX29"/>
    <dbReference type="STRING" id="348780.NP_3146A"/>
    <dbReference type="EnsemblBacteria" id="CAI49664">
        <property type="protein sequence ID" value="CAI49664"/>
        <property type="gene ID" value="NP_3146A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701668"/>
    <dbReference type="KEGG" id="nph:NP_3146A"/>
    <dbReference type="eggNOG" id="arCOG06388">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134823_0_0_2"/>
    <dbReference type="OrthoDB" id="193961at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="3"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="168" mass="18593" checksum="76D7258C71351E82" modified="2017-05-10" version="1">MIFVVPFDGTSLAETALVRAVEFSTVLDEQVLAVTAIPRGNAEYARERGWIDEEEPFDRQQIVTTLHEQVVNLAPEADFRHATVGKYAPPGTIATRIRELSTEVSPSMFFIGSENAGRVVSTVSSVGAKVASEKNYDVVIVRNHAPTRVEKLRQSSPHRSSKSDFYQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX47</accession>
    <name>A0A1U7EX47_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Electron transfer flavoprotein beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">etfB</name>
        <name evidence="5" type="ordered locus">NP_3170A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ETF beta-subunit/FixA family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323298.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX47"/>
    <dbReference type="STRING" id="348780.NP_3170A"/>
    <dbReference type="EnsemblBacteria" id="CAI49676">
        <property type="protein sequence ID" value="CAI49676"/>
        <property type="gene ID" value="NP_3170A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702679"/>
    <dbReference type="KEGG" id="nph:NP_3170A"/>
    <dbReference type="eggNOG" id="arCOG00446">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060196_1_1_2"/>
    <dbReference type="OrthoDB" id="51427at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01714">
        <property type="entry name" value="ETF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012255">
        <property type="entry name" value="ETF_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033948">
        <property type="entry name" value="ETF_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN BETA-SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294:SF8">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000090">
        <property type="entry name" value="Beta-ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Electron transfer flavoprotein alpha/beta-subunit N-terminal" evidence="4">
        <location>
            <begin position="21"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="264" mass="28611" checksum="BF3030CD9607F601" modified="2017-05-10" version="1">MKILVTVKEVAEVDDEFDISGLDIDEGSLEYDLNEWDDYAVEEAVQISEERDDVEVVTVTIGPERADETIRMALAKGADRAIRVWDDALEGTQFLDVDAKAEIIAAVAEAEDPDFVLSGVQAGDDSNGATGVALAELLDFEWGAVVNDLDLDDAAETASVHRELEGGIEELTDVDLPAVLTIQTGINEPRYASLRGIRQAQRKPLDVQDLSELGLDADVVESPVERTEMYEPESEGDATIWEGDADQTAGELAAFLRDKGVVEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX59</accession>
    <name>A0A1U7EX59_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Orc1-type DNA replication protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">orc4</name>
        <name evidence="6" type="synonym">cdc6D</name>
        <name evidence="6" type="ordered locus">NP_3258A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDC6/cdc18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323341.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX59"/>
    <dbReference type="STRING" id="348780.NP_3258A"/>
    <dbReference type="EnsemblBacteria" id="CAI49720">
        <property type="protein sequence ID" value="CAI49720"/>
        <property type="gene ID" value="NP_3258A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702593"/>
    <dbReference type="KEGG" id="nph:NP_3258A"/>
    <dbReference type="eggNOG" id="arCOG00467">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025112_0_0_2"/>
    <dbReference type="OrthoDB" id="270161at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049945">
        <property type="entry name" value="AAA_22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055237">
        <property type="entry name" value="Cdc6_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050311">
        <property type="entry name" value="ORC1/CDC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014277">
        <property type="entry name" value="Orc1/Cdc6_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02928">
        <property type="entry name" value="orc1/cdc6 family replication initiation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763:SF22">
        <property type="entry name" value="ORC1-TYPE DNA REPLICATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13401">
        <property type="entry name" value="AAA_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22703">
        <property type="entry name" value="Cdc6_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="39"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI49720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="334" mass="37962" checksum="B654DF5F905C1742" modified="2017-05-10" version="1">MIVDARALDVEAVPSEILRRNNELNALSNALEPLLDGYRGEHSIVVGPSGAGKTACARYTVEQLRRSLVDVRAQYINCWQHSSRFAALLQLVDGIGQSMDIQQHSTPHDELLRRLRNVDDRPYIVILDEADQLDCREDILYELTTLDHVYMILICNRERELLDGLDRRVVSRLRGCRRIEFEAYSTATLVDILDRRAEVGLEAGSLTDGVLREIAEVSNGDARVAIRTLAEAAKQAERDSTSIKSDYVDEAVSEAEENIRQKAISQLNRHQRVIYDVLEEDGGWLPMGELYPEYSERVDEARTKTTVSNYLSKIAEYNLVELKGEKRGRRYRAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX69</accession>
    <name>A0A1U7EX69_NATPD</name>
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        <submittedName>
            <fullName evidence="2">CRISPR-associated helicase Cas3</fullName>
            <ecNumber evidence="2">3.6.4.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cas3</name>
        <name evidence="2" type="ordered locus">NP_3270A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.4.-" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323347.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX69"/>
    <dbReference type="STRING" id="348780.NP_3270A"/>
    <dbReference type="EnsemblBacteria" id="CAI49726">
        <property type="protein sequence ID" value="CAI49726"/>
        <property type="gene ID" value="NP_3270A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701710"/>
    <dbReference type="KEGG" id="nph:NP_3270A"/>
    <dbReference type="eggNOG" id="arCOG03483">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_397741_0_0_2"/>
    <dbReference type="OrthoDB" id="36796at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120545">
        <property type="term" value="F:nucleic acid conformation isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09710">
        <property type="entry name" value="Cas3_I-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052511">
        <property type="entry name" value="ATP-dep_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017575">
        <property type="entry name" value="CRISPR-assoc_helicase_Cas3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03158">
        <property type="entry name" value="cas3_cyano"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47962:SF5">
        <property type="entry name" value="ATP-DEPENDENT HELICASE LHR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47962">
        <property type="entry name" value="ATP-DEPENDENT HELICASE LHR-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="1">
        <location>
            <begin position="54"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="716" mass="81277" checksum="7919190CF15CD703" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX85</accession>
    <name>A0A1U7EX85_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transducer protein Htr33</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">htr33</name>
        <name evidence="6" type="ordered locus">NP_3374A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323398.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX85"/>
    <dbReference type="STRING" id="348780.NP_3374A"/>
    <dbReference type="EnsemblBacteria" id="CAI49778">
        <property type="protein sequence ID" value="CAI49778"/>
        <property type="gene ID" value="NP_3374A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702847"/>
    <dbReference type="KEGG" id="nph:NP_3374A"/>
    <dbReference type="eggNOG" id="arCOG02325">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_116_1_2"/>
    <dbReference type="OrthoDB" id="8523at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11386">
        <property type="entry name" value="MCP_signal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.950">
        <property type="entry name" value="Methyl-accepting chemotaxis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004090">
        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32089:SF112">
        <property type="entry name" value="LYSOZYME-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32089">
        <property type="entry name" value="METHYL-ACCEPTING CHEMOTAXIS PROTEIN MCPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00260">
        <property type="entry name" value="CHEMTRNSDUCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58104">
        <property type="entry name" value="Methyl-accepting chemotaxis protein (MCP) signaling domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="Methyl-accepting transducer" evidence="5">
        <location>
            <begin position="171"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="396"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI49778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="444" mass="48373" checksum="2E4D920A4ABD48A3" modified="2017-05-10" version="1">MSSQPAEGEPDEDGLPSSHFELSEEFEALDSTEAKVMLQERTKELSAIQRANELFGNVDQPVEALAEEYVTELPQWFQYPSVTEAEIQVGDQLFQSSGYQPTAHPLTSEETTDAGTTVRIQIVYTEDRPAEDDGPWLDEEQEMLDTLDGFIRNYVAQLETRTQVETQVQSSVQEVRQTADNIAESSEQISDYARQQAESMNDVGNEVSGMSATIEEIASTTDEVAESSIRAENLTGEGQDAVTDAVDAMEAIDASRAEVSDDIHDLESHVDEIDEVVEIIHDIAEETNILALNANIEAARAGDAGSGFAVVADEVKSLAEDSQSYASDIETIVEQVKQKTDESVSSLETTAQQVDRGLEQVEEAKDRLSEIADSVGEVTEGVQDVAAATDEQATSSEEVASMVDELTEQAEEIADEIESIAAANEEQVSKVEEIDEAVEQLSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX90</accession>
    <name>A0A1U7EX90_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3380A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323401.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX90"/>
    <dbReference type="STRING" id="348780.NP_3380A"/>
    <dbReference type="EnsemblBacteria" id="CAI49781">
        <property type="protein sequence ID" value="CAI49781"/>
        <property type="gene ID" value="NP_3380A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701757"/>
    <dbReference type="KEGG" id="nph:NP_3380A"/>
    <dbReference type="eggNOG" id="arCOG04599">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067490_0_0_2"/>
    <dbReference type="OrthoDB" id="293478at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043812">
        <property type="entry name" value="DUF5794"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19107">
        <property type="entry name" value="DUF5794"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="82"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="267"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="305" mass="31151" checksum="E470A017032627B2" modified="2017-05-10" version="1">MSTSRHPVALRLERRVGEGTRLLGTVMALPLLDGIFAALVVAGAVSSVLGMIEVGLLIFGGSATLAVVLAEMEGSRRDRVRNILLVGALVVPFAAVQAAIAPTIESLLAMDIFKRFAALVILAVAAKTASATIGEYLPGPGVIIALGLVASFQPNGFAVAVVAEPMYVVYGTGAALVGVGFALAVALLGPWLRGNVDIDRFRFGSAVALGVLPLTILEVGPITGEEPLALIVLALTGLLAFNPDGALEIGPQETPDTGVPAATDGSGVDDESPLEDPLDEPSGSDDDQSEDGEPGVSDGDRAPWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXA0</accession>
    <name>A0A1U7EXA0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DUF35 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3418A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323420.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXA0"/>
    <dbReference type="STRING" id="348780.NP_3418A"/>
    <dbReference type="EnsemblBacteria" id="CAI49800">
        <property type="protein sequence ID" value="CAI49800"/>
        <property type="gene ID" value="NP_3418A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701771"/>
    <dbReference type="KEGG" id="nph:NP_3418A"/>
    <dbReference type="eggNOG" id="arCOG01285">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1691489_0_0_2"/>
    <dbReference type="OrthoDB" id="9573at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00164">
        <property type="entry name" value="S1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.30.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002878">
        <property type="entry name" value="ChsH2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022002">
        <property type="entry name" value="ChsH2_Znr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052513">
        <property type="entry name" value="Thioester_dehydratase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34075">
        <property type="entry name" value="BLR3430 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34075:SF5">
        <property type="entry name" value="BLR3430 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01796">
        <property type="entry name" value="OB_ChsH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12172">
        <property type="entry name" value="zf-ChsH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ChsH2 rubredoxin-like zinc ribbon" evidence="2">
        <location>
            <begin position="21"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="ChsH2 C-terminal OB-fold" evidence="1">
        <location>
            <begin position="60"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="147" mass="15973" checksum="D3BA0462B321F269" modified="2017-05-10" version="1">MSDRADRPLDAESPLTLPGFFAALDDGELFGGVCTDCGAVLFPPRPTCYNCGGTNVSAEPQPKTGTVYTYTAVHSPPPQLDDDAPYTIAVVELDGGGRLLGRVDAPYEAVEIGDEVRLRVREPTDDERALALEYEREWPMHVFDLDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXA3</accession>
    <name>A0A1U7EXA3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF457 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3400A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323411.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXA3"/>
    <dbReference type="STRING" id="348780.NP_3400A"/>
    <dbReference type="EnsemblBacteria" id="CAI49791">
        <property type="protein sequence ID" value="CAI49791"/>
        <property type="gene ID" value="NP_3400A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701764"/>
    <dbReference type="KEGG" id="nph:NP_3400A"/>
    <dbReference type="eggNOG" id="arCOG01744">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133604_0_0_2"/>
    <dbReference type="OrthoDB" id="118042at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007404">
        <property type="entry name" value="YdjM-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04307">
        <property type="entry name" value="YdjM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="160" mass="16185" checksum="BB77E7D051BF7522" modified="2017-05-10" version="1">MHRAGHVGAALLTYVPFGFVLSAAEPALAILGGVGVVVLSRLPDIDFVLPGVEHRGATHTVGFLLVVTAVLAGVGFVISGTFGTGTTPGQSAVLGALIGATAVGSHLLADMLAPPGLPLLWPVSRAQFTVNVTHPRDPLANYGLLTFGVVLTVLVGYVAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXA6</accession>
    <name>A0A1U7EXA6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC-type transport system ATP-binding protein (Probable substrate branched-chain amino acids)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">livG4</name>
        <name evidence="5" type="synonym">abc08a2</name>
        <name evidence="5" type="ordered locus">NP_3430A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323426.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXA6"/>
    <dbReference type="STRING" id="348780.NP_3430A"/>
    <dbReference type="EnsemblBacteria" id="CAI49806">
        <property type="protein sequence ID" value="CAI49806"/>
        <property type="gene ID" value="NP_3430A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702388"/>
    <dbReference type="KEGG" id="nph:NP_3430A"/>
    <dbReference type="eggNOG" id="arCOG00926">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OrthoDB" id="44250at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015188">
        <property type="term" value="F:L-isoleucine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015192">
        <property type="term" value="F:L-phenylalanine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005304">
        <property type="term" value="F:L-valine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042941">
        <property type="term" value="P:D-alanine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015808">
        <property type="term" value="P:L-alanine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903806">
        <property type="term" value="P:L-isoleucine import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903805">
        <property type="term" value="P:L-valine import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03219">
        <property type="entry name" value="ABC_Mj1267_LivG_branched"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051120">
        <property type="entry name" value="ABC_AA/LPS_Transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45772:SF7">
        <property type="entry name" value="AMINO ACID ABC TRANSPORTER ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45772">
        <property type="entry name" value="CONSERVED COMPONENT OF ABC TRANSPORTER FOR NATURAL AMINO ACIDS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="7"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="252" mass="28279" checksum="B070B75245FC5B33" modified="2017-05-10" version="1">MSSTPILETVDVRKEFGNLVAVDGISLEFYTDETVGIIGPNGAGKTTFINLISGVLPLTDGQILFAGDDISDLKRYKRVRRGLVRSFQIPQIYDELTLRENIQSSILSRKQENNRLFTPTDREEESLQEANELIETFNLEAHADEHTEHLPHGVRKVLDVAMSFALEPQIMFLDEPTSGVGSKEKNDVMETITQAADEQNIGLVFIEHDMELIREYSSRTVALHEGRVLLDDEPDAVFNSEEVNEFILEGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXB9</accession>
    <name>A0A1U7EXB9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Probable prenyl carboxy-lyase</fullName>
            <ecNumber evidence="5">4.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_3414A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UbiD family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323418.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXB9"/>
    <dbReference type="STRING" id="348780.NP_3414A"/>
    <dbReference type="EnsemblBacteria" id="CAI49798">
        <property type="protein sequence ID" value="CAI49798"/>
        <property type="gene ID" value="NP_3414A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703438"/>
    <dbReference type="KEGG" id="nph:NP_3414A"/>
    <dbReference type="eggNOG" id="arCOG01671">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023348_0_0_2"/>
    <dbReference type="OrthoDB" id="8480at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046281">
        <property type="term" value="P:cinnamic acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033494">
        <property type="term" value="P:ferulate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1670.10">
        <property type="entry name" value="UbiD C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002830">
        <property type="entry name" value="UbiD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049381">
        <property type="entry name" value="UbiD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049383">
        <property type="entry name" value="UbiD-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048304">
        <property type="entry name" value="UbiD_Rift_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30108">
        <property type="entry name" value="3-OCTAPRENYL-4-HYDROXYBENZOATE CARBOXY-LYASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30108:SF17">
        <property type="entry name" value="FERULIC ACID DECARBOXYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01977">
        <property type="entry name" value="UbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20696">
        <property type="entry name" value="UbiD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20695">
        <property type="entry name" value="UbiD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143968">
        <property type="entry name" value="UbiD C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3-octaprenyl-4-hydroxybenzoate carboxy-lyase-like N-terminal" evidence="3">
        <location>
            <begin position="11"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="3-octaprenyl-4-hydroxybenzoate carboxy-lyase-like Rift-related" evidence="2">
        <location>
            <begin position="108"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="3-octaprenyl-4-hydroxybenzoate carboxy-lyase-like C-terminal" evidence="4">
        <location>
            <begin position="347"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="504" mass="55429" checksum="0ACF8634D2A6B8C5" modified="2017-05-10" version="1">MSATESFRGFLSALDAAGELNRIQQPVSWDLEAAAITTRANERDAAIPVFETMSGDAASARLVGDPYRGPPTRPWANVARGAGIDPRSDSTTFFEAMVERLESRIEPKTVERDEAPCKAVVNRGSDADLLDLPWPYIHQGDGGRYSSLHTLVAPDPTTEWGRCGDHRAMIHGTQRASVRLLAGEQIPNRYYYDYERGGEPMPVAIAIGPPPAVSFTSNLWIPTGRSEFEFAGSIRGSAIELVECETNDLYVPATAEIVIEGHIHPDDRLDEGPYGDYLGFMHGPRRSMPALDVAAITHRPDPYLPFCVDGSGTGYPFNSSNAIEHACSGPDATIGARAAGFDIERSATWPYTEQTVFVFATEEQYDGYFHDVANFVFTSWGMLRVDFFIFVDADVDPMDPRAVFEAIALHADPEEDFYQFGIERMPKTPLNIYQTPEEKGSADLGTSKTKTAKAYIDATRAENAEGVEQTVYSDRRLRERAQETLATAGFDASKLPLSSGRGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXC8</accession>
    <name>A0A1U7EXC8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UspA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3436A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323429.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXC8"/>
    <dbReference type="STRING" id="348780.NP_3436A"/>
    <dbReference type="EnsemblBacteria" id="CAI49809">
        <property type="protein sequence ID" value="CAI49809"/>
        <property type="gene ID" value="NP_3436A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701772"/>
    <dbReference type="KEGG" id="nph:NP_3436A"/>
    <dbReference type="eggNOG" id="arCOG03050">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_19_1_2"/>
    <dbReference type="OrthoDB" id="281037at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF6">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN UP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="3"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="139" mass="15248" checksum="0269B0BD369BCF07" modified="2017-05-10" version="1">MYRVLMPVDTDEERSLKQAEYVSSLPGSDTDVEAIILFIFQDDDEELPEGFGSFKSATRVGSVRRVSEHFDEHDVAYTVLDDAGETADRIIEEANEHDVDSIVLGGRKQSPVGKALFGSVTQSVILDSDRPVVVTGADK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXE0</accession>
    <name>A0A1U7EXE0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA gyrase subunit B</fullName>
            <ecNumber evidence="10">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 13" type="primary">gyrB</name>
        <name evidence="13" type="ordered locus">NP_3498A</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="10">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="10">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="10">Binds two Mg(2+) per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn(2+) or Ca(2+).</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="10">Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the type II topoisomerase GyrB family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="10"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323460.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXE0"/>
    <dbReference type="STRING" id="348780.NP_3498A"/>
    <dbReference type="EnsemblBacteria" id="CAI49840">
        <property type="protein sequence ID" value="CAI49840"/>
        <property type="gene ID" value="NP_3498A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702790"/>
    <dbReference type="KEGG" id="nph:NP_3498A"/>
    <dbReference type="eggNOG" id="arCOG04371">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006146_4_1_2"/>
    <dbReference type="OrthoDB" id="358756at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034335">
        <property type="term" value="F:DNA negative supercoiling activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16928">
        <property type="entry name" value="HATPase_GyrB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00822">
        <property type="entry name" value="TopoII_Trans_DNA_gyrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03366">
        <property type="entry name" value="TOPRIM_TopoIIA_GyrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000005">
        <property type="entry name" value="DNA gyrase subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000002">
        <property type="entry name" value="DNA gyrase subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.670:FF:000002">
        <property type="entry name" value="DNA gyrase subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
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</entry>
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            <end position="234"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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        </location>
    </feature>
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        <location>
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        <location>
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        <location>
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        </location>
    </feature>
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        <location>
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            <end position="153"/>
        </location>
    </feature>
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        <location>
            <begin position="281"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoside transporter/FeoB GTPase Gate" evidence="3">
        <location>
            <begin position="439"/>
            <end position="539"/>
        </location>
    </feature>
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        <source>
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        <source>
            <dbReference type="Pfam" id="PF02421"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF07670"/>
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        <source>
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    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="569" mass="60753" checksum="BE01F8E5698E6CB2" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXI4</accession>
    <name>A0A1U7EXI4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UspA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3600A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXI4"/>
    <dbReference type="STRING" id="348780.NP_3600A"/>
    <dbReference type="EnsemblBacteria" id="CAI49891">
        <property type="protein sequence ID" value="CAI49891"/>
        <property type="gene ID" value="NP_3600A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3600A"/>
    <dbReference type="eggNOG" id="arCOG02053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_11_1_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF6">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN UP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="2"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="151" mass="16378" checksum="3B8C8C3593E23D9E" modified="2017-05-10" version="1">MERVLLPTDGSDCAETAAGLASRIASDYDATLHVLHISETVPGKVDGPPILDADLEATDCEAIQRIHQQARGYGAEDIEAAISSGYPFREIVRYVELRDIDIVVMGSHDRTGLGRVLNRSTTKNVIRRSPAPVLTVPQSTEESAMLPIPEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXP7</accession>
    <name>A0A1U7EXP7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Probable rhomboid family protease</fullName>
            <ecNumber evidence="10">3.4.21.105</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">NP_3724A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S54 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.105" evidence="10"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323570.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXP7"/>
    <dbReference type="STRING" id="348780.NP_3724A"/>
    <dbReference type="EnsemblBacteria" id="CAI49953">
        <property type="protein sequence ID" value="CAI49953"/>
        <property type="gene ID" value="NP_3724A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701848"/>
    <dbReference type="KEGG" id="nph:NP_3724A"/>
    <dbReference type="eggNOG" id="arCOG01768">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111002_0_0_2"/>
    <dbReference type="OrthoDB" id="169619at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43066:SF1">
        <property type="entry name" value="RHOMBOID PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43066">
        <property type="entry name" value="RHOMBOID-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 10" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="46"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="108"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="145"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="9">
        <location>
            <begin position="41"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI49953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="192" mass="18915" checksum="4C4A8CBCB26DFE9F" modified="2017-05-10" version="1">MSSRPTLQALAACVVVFLLQQAGGAVGLGDAVFALALPLSSQPWTLVVSVYAHGGLTHLLANAVALVLIGPLVARVTTPARFHAFFIITGALAGIAQVLVLMPFGGGAVLGASGAIFALFGYLLVGNRGTGRLLSVVALGTRGKLVVLAVLAALLTAATATPGAALVAHFVGFVLGAAAGGSRLLHTSGSAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXT7</accession>
    <name>A0A1U7EXT7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PD-(D/E)XK endonuclease-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3870A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323643.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXT7"/>
    <dbReference type="STRING" id="348780.NP_3870A"/>
    <dbReference type="EnsemblBacteria" id="CAI50026">
        <property type="protein sequence ID" value="CAI50026"/>
        <property type="gene ID" value="NP_3870A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701895"/>
    <dbReference type="KEGG" id="nph:NP_3870A"/>
    <dbReference type="eggNOG" id="arCOG06435">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_331403_0_0_2"/>
    <dbReference type="OrthoDB" id="319934at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038726">
        <property type="entry name" value="PDDEXK_AddAB-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12705">
        <property type="entry name" value="PDDEXK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PD-(D/E)XK endonuclease-like" evidence="2">
        <location>
            <begin position="480"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="451"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="862" mass="97966" checksum="84710BF24FA89FD6" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXT8</accession>
    <name>A0A1U7EXT8_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 10">NH(3)-dependent NAD(+) synthetase</fullName>
            <ecNumber evidence="8 10">6.3.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">nadE1</name>
        <name evidence="8" type="synonym">nadE</name>
        <name evidence="12" type="ordered locus">NP_3828A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses ammonia as a nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>deamido-NAD(+) + NH4(+) + ATP = AMP + diphosphate + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21188"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 9">Belongs to the NAD synthetase family.</text>
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        <property type="protein sequence ID" value="CAI50005.1"/>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
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    <dbReference type="KEGG" id="nph:NP_3828A"/>
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        <property type="taxonomic scope" value="Archaea"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:glutaminase activity"/>
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        <property type="entry name" value="NH 3 /GLUTAMINE-DEPENDENT NAD + SYNTHETASE"/>
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        <property type="match status" value="1"/>
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    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
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    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8 9" id="KW-0520">NAD</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD/GMP synthase" evidence="11">
        <location>
            <begin position="28"/>
            <end position="269"/>
        </location>
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        <location>
            <begin position="48"/>
            <end position="55"/>
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        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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    <feature type="binding site" evidence="8">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
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        <location>
            <position position="133"/>
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            <position position="153"/>
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            <position position="158"/>
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            <dbReference type="ChEBI" id="CHEBI:18420"/>
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    <feature type="binding site" description="in other chain" evidence="8">
        <location>
            <position position="166"/>
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        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <note>ligand shared between two neighboring subunits</note>
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        <location>
            <position position="182"/>
        </location>
        <ligand>
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            <position position="204"/>
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        <ligand>
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        <location>
            <begin position="264"/>
            <end position="265"/>
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            <name>deamido-NAD(+)</name>
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        <source>
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            <dbReference type="ARBA" id="ARBA00022598"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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            <dbReference type="RuleBase" id="RU003812"/>
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            <dbReference type="Pfam" id="PF02540"/>
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        <source>
            <dbReference type="EMBL" id="CAI50005.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
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    <sequence length="275" mass="29671" checksum="74B73BD7478FC35E" modified="2017-05-10" version="1">MTASEPTIEGTNVELLFSESELQRRRDHIVEFIEETIETAGADRAVLGLSGGIDSTTVAYLAVAALGPDRVHGISMPSSVNPDDDETDAERVANALDIEFDVIDINPIVDAFVDAAPDHAADDRMALGNVRVRTRAVLNYFVANAEDGLVLGTGNRSEAATGYFTKYGDQAVDCNPIGNLYKCQVRQLARELGVPEDLVSRTPTAAMWEGQTDEEEMGLGYDELDAILAVHVDGPLSKSATVRTLDVPESAVDRVVELYETSTHKRAMPPAPEPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY10</accession>
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        <name evidence="5" type="ordered locus">NP_4022A</name>
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        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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            <taxon>Natronomonas</taxon>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
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                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
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            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
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            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
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        <text evidence="1">Belongs to the GSP E family.</text>
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    </dbReference>
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        <property type="entry name" value="PilB3-like_N"/>
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        <property type="entry name" value="T4SS_GSP_E_ATPase"/>
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        <property type="entry name" value="TWITCHING MOTILITY PROTEIN PILT"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR30486:SF6">
        <property type="entry name" value="TYPE IV PILUS RETRACTATION ATPASE PILT"/>
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    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="125"/>
            <end position="164"/>
        </location>
    </feature>
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        <location>
            <begin position="391"/>
            <end position="603"/>
        </location>
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        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="2">
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            <begin position="262"/>
            <end position="310"/>
        </location>
    </feature>
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        <location>
            <begin position="779"/>
            <end position="842"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="830"/>
            <end position="842"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00006611"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI50102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="842" mass="93233" checksum="79AB2F594793A747" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY26</accession>
    <name>A0A1U7EY26_NATPD</name>
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        <submittedName>
            <fullName evidence="2">DUF88 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4076A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
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            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
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    <dbReference type="STRING" id="348780.NP_4076A"/>
    <dbReference type="EnsemblBacteria" id="CAI50129">
        <property type="protein sequence ID" value="CAI50129"/>
        <property type="gene ID" value="NP_4076A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701961"/>
    <dbReference type="KEGG" id="nph:NP_4076A"/>
    <dbReference type="eggNOG" id="arCOG02408">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092340_3_0_2"/>
    <dbReference type="OrthoDB" id="295810at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:RNA nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="PIN_LabA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LabA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021139">
        <property type="entry name" value="NYN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35458:SF8">
        <property type="entry name" value="SLR0650 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35458">
        <property type="entry name" value="SLR0755 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01936">
        <property type="entry name" value="NYN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NYN" evidence="1">
        <location>
            <begin position="9"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="165" mass="18565" checksum="19FC6DD3FA1DB3DD" modified="2017-05-10" version="1">MPPIHSNQRVAVLADSQNLYHTAHSYYSRNPDYTELLSAAVRDRELIRAIAYVIRADPPTEQEFFEALRDIGFETKIKDIKTFADGTQKANWDLGMCLDAVTLAPKIDTFVLASGDGDFARLCTHLRHEGVRTEVFGFGDSTAEELIDAADSYVDMSEDEDRFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY28</accession>
    <name>A0A1U7EY28_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">2-oxoglutarate synthase subunit KorA</fullName>
            <ecNumber evidence="4">1.2.7.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">2-ketoglutarate oxidoreductase alpha chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">2-oxoglutarate-ferredoxin oxidoreductase subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">porA</name>
        <name evidence="12" type="ordered locus">NP_4046A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2 oxidized [2Fe-2S]-[ferredoxin] + 2-oxoglutarate + CoA = succinyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17297"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="EC" id="1.2.7.3"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterotetramer of the KorA, KorB, KorC and KorD subunits.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.3" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323730.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY28"/>
    <dbReference type="STRING" id="348780.NP_4046A"/>
    <dbReference type="EnsemblBacteria" id="CAI50114">
        <property type="protein sequence ID" value="CAI50114"/>
        <property type="gene ID" value="NP_4046A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703043"/>
    <dbReference type="KEGG" id="nph:NP_4046A"/>
    <dbReference type="eggNOG" id="arCOG01606">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017038_1_0_2"/>
    <dbReference type="OrthoDB" id="31112at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000022">
        <property type="entry name" value="2-oxoglutarate ferredoxin oxidoreductase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022367">
        <property type="entry name" value="2-oxoacid/accept_OxRdtase_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033412">
        <property type="entry name" value="PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050722">
        <property type="entry name" value="Pyruvate:ferred/Flavod_OxRd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03710">
        <property type="entry name" value="OAFO_sf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32154:SF20">
        <property type="entry name" value="2-OXOGLUTARATE OXIDOREDUCTASE SUBUNIT KORA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32154">
        <property type="entry name" value="PYRUVATE-FLAVODOXIN OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17147">
        <property type="entry name" value="PFOR_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate/ketoisovalerate oxidoreductase catalytic" evidence="9">
        <location>
            <begin position="14"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate flavodoxin/ferredoxin oxidoreductase pyrimidine binding" evidence="10">
        <location>
            <begin position="231"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate:ferredoxin oxidoreductase core" evidence="11">
        <location>
            <begin position="508"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="427"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="431"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00052359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00064882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00066947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00071398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00076968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00079587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI50114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="629" mass="68592" checksum="36DAF95A3945814A" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYA2</accession>
    <name>A0A1U7EYA2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glyoxalase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4204A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323809.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYA2"/>
    <dbReference type="EnsemblBacteria" id="CAI50193">
        <property type="protein sequence ID" value="CAI50193"/>
        <property type="gene ID" value="NP_4204A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702003"/>
    <dbReference type="KEGG" id="nph:NP_4204A"/>
    <dbReference type="eggNOG" id="arCOG03102">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1127078_0_0_2"/>
    <dbReference type="OrthoDB" id="304574at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06587">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="4"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="126"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="236" mass="26201" checksum="08179C54E14C86E0" modified="2017-05-10" version="1">MLGDCRWLTLEVTSLDSVAPFYRERLSLPLIEATDRQYRFDAGGSSELRLRRPTAVPRGGLHTHYAFSCPADAYDAWWDRLDADFDLQEVDFGSMRSLYLYDPAGNCVEIAGADEPSGTAGRTLSGIFEVVFEVADLPASEQFYTALGFEVVDRGENRTRTRLTNGPLDIELWEPQLGLADARGGVHVDVGINAESPAAVADNVAERVTKREQLDDGAVRIRDPDGHYLTFVRSER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYA8</accession>
    <name>A0A1U7EYA8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Mechanosensitive channel protein MscS</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">mscS6</name>
        <name evidence="8" type="ordered locus">NP_4196A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323805.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYA8"/>
    <dbReference type="STRING" id="348780.NP_4196A"/>
    <dbReference type="EnsemblBacteria" id="CAI50189">
        <property type="protein sequence ID" value="CAI50189"/>
        <property type="gene ID" value="NP_4196A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702943"/>
    <dbReference type="KEGG" id="nph:NP_4196A"/>
    <dbReference type="eggNOG" id="arCOG01570">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_982141_0_0_2"/>
    <dbReference type="OrthoDB" id="313107at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
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        </location>
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            <begin position="148"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="195"/>
        </location>
    </feature>
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        <location>
            <begin position="197"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI50189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="264" mass="28028" checksum="C0D4F8BDE4FD35D2" modified="2017-05-10" version="1">MVDLPLRSWFEGLPTPGQNLLVAAGVLVAGLVAGALTAYLVSRALRAFGVDDAVEGTPFERTARQLGTSTVRLLARLCGLFVFVVAALYAGQVLEVMPEEAALERISAFLPQLFVAVLVILVGLIAADKAELVVSERLKSIKLPEVTLIPTLVKVSILYVAALIALSQLGVATGALLILLAAYAFGVFFLGGLAFKDLLSSAAAGIYVLMTEPYSIGDEVAIDGCRGIVQEVDVFVTYVEAEGEEYMIPNRKVLREGAMRVRSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYE4</accession>
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        <submittedName>
            <fullName evidence="2">Homolog to ribonuclease Z</fullName>
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        <name evidence="2" type="ordered locus">NP_4346A</name>
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        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
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            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAI50264.1"/>
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        <property type="gene ID" value="NP_4346A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702038"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="F:3'-tRNA processing endoribonuclease activity"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46018:SF3">
        <property type="entry name" value="ARYLSULFATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46018">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
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    </dbReference>
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            <begin position="18"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="252" mass="26509" checksum="18D97B4F8E62A8D7" modified="2017-05-10" version="1">MRVTFLGTGAAMPSGDRFQTGLLLEDSGGTASPLLVDCGSGVLHGLARTDGGYEAPGTVLLTHHHLDHVADLLPLLKARWLAGETDLELVGPAGTAALIEDLLSVHEYLQGRVAITVRELSAGSATVAGYDVTAVETDHSMDGLAYRFETDDAIFTFSGDTEPIDEIAALAEGSAVLAHDCSFPDGVDVDNHPTPSRLGETLSGYDIGRVYLTHLYPHTDGHHEEMVAAVEDCFDGDVRIANDGLTVSISGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYE5</accession>
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        <submittedName>
            <fullName evidence="3">Cox-type terminal oxidase subunit I</fullName>
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        </submittedName>
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        <name evidence="3" type="primary">coxA3</name>
        <name evidence="3" type="ordered locus">NP_4296A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
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            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.9.3.-" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYE5"/>
    <dbReference type="STRING" id="348780.NP_4296A"/>
    <dbReference type="EnsemblBacteria" id="CAI50239">
        <property type="protein sequence ID" value="CAI50239"/>
        <property type="gene ID" value="NP_4296A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702625"/>
    <dbReference type="KEGG" id="nph:NP_4296A"/>
    <dbReference type="eggNOG" id="arCOG01238">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033807_0_0_2"/>
    <dbReference type="OrthoDB" id="28065at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.210.10">
        <property type="entry name" value="Cytochrome c oxidase-like, subunit I domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023616">
        <property type="entry name" value="Cyt_c_oxase-like_su1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036927">
        <property type="entry name" value="Cyt_c_oxase-like_su1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000883">
        <property type="entry name" value="Cyt_C_Oxase_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422:SF40">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00115">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81442">
        <property type="entry name" value="Cytochrome c oxidase subunit I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50855">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="248"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="311"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="357"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="402"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="434"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="483"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="546"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome oxidase subunit I profile" evidence="2">
        <location>
            <begin position="38"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="575" mass="62486" checksum="67269EE3775A2565" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYF8</accession>
    <name>A0A1U7EYF8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">UPF0058 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4316A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323865.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYF8"/>
    <dbReference type="EnsemblBacteria" id="CAI50249">
        <property type="protein sequence ID" value="CAI50249"/>
        <property type="gene ID" value="NP_4316A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702028"/>
    <dbReference type="KEGG" id="nph:NP_4316A"/>
    <dbReference type="eggNOG" id="arCOG02254">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.110">
        <property type="entry name" value="Uncharacterised protein family UPF0058"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002753">
        <property type="entry name" value="UPF0058"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036519">
        <property type="entry name" value="UPF0058_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42203">
        <property type="entry name" value="UPF0058 PROTEIN MJ1205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42203:SF2">
        <property type="entry name" value="UPF0058 PROTEIN MJ1205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01893">
        <property type="entry name" value="UPF0058"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140371">
        <property type="entry name" value="Vng1086c-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="78" mass="8591" checksum="B126E330F6C9AF6A" modified="2017-05-10" version="1">MKKQELIHLHGLLAEVCNHYEQIAECNVEHAKYTELGVRPTSIHKSKTDHKAAVFALADGITEEMESETETAVSAAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYG4</accession>
    <name>A0A1U7EYG4_NATPD</name>
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        <submittedName>
            <fullName evidence="3">DUF4013 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_4342A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323878.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYG4"/>
    <dbReference type="STRING" id="348780.NP_4342A"/>
    <dbReference type="EnsemblBacteria" id="CAI50262">
        <property type="protein sequence ID" value="CAI50262"/>
        <property type="gene ID" value="NP_4342A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702037"/>
    <dbReference type="KEGG" id="nph:NP_4342A"/>
    <dbReference type="eggNOG" id="arCOG02879">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_738926_0_0_2"/>
    <dbReference type="OrthoDB" id="107590at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025098">
        <property type="entry name" value="DUF4013"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13197">
        <property type="entry name" value="DUF4013"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="81"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="134"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="177"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="206"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="266"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="297"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="331" mass="34897" checksum="B63DBA4142C3612C" modified="2017-05-10" version="1">MFREAMGYPTRQPNGGRVVFAGGALLAVVFVAVAMTQFGWPYGLVAVIGLLPWLLVRGYYVRVIRTTIGRDAPLPPSFSNWRALLADGATAVGISVAYMLPAAIILAPLAAVQLLGNDLSAPISALPTPVVDGVLSGVGFLAVFALMYLLGALYVLPVAVARFAYTDRVSAAFDIRAVVAGALTEDYATAWFVSVALQFFVLPVAYLLRIILVGFLLQFVVAVAVRYCYGQGVGDAMGWDPVDPDGGPVPWNELTPAVTRVAEAGAKRATTRRNTTRAGRWPDTEPAVARIKAPASDGGGEDNGNGDTNGGQEAEGDWVPASSVTIRRNDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYI1</accession>
    <name>A0A1U7EYI1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7965 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_4394A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323904.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYI1"/>
    <dbReference type="STRING" id="348780.NP_4394A"/>
    <dbReference type="EnsemblBacteria" id="CAI50288">
        <property type="protein sequence ID" value="CAI50288"/>
        <property type="gene ID" value="NP_4394A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702048"/>
    <dbReference type="KEGG" id="nph:NP_4394A"/>
    <dbReference type="eggNOG" id="arCOG11098">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1640013_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058271">
        <property type="entry name" value="DUF7965"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25913">
        <property type="entry name" value="DUF7965"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7965" evidence="2">
        <location>
            <begin position="7"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="161" mass="15709" checksum="B7719FF4B7B856C8" modified="2017-05-10" version="1">MAEPADRLAVWTAGTAALVVVTVGALLAGHLVGVLSTVLPAIGTAAAGLLFGYLWLLSLVAAGFVDVLDAKDGYPFAIARTAAAGGGVAVVYLTVVLVVGFVSPTPTPVEPAPAVVARTVVVGASIGAVSGGLFAAFGLACGRIGRAVADGCVEAGDGRRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYJ7</accession>
    <name>A0A1U7EYJ7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4422A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYJ7"/>
    <dbReference type="EnsemblBacteria" id="CAI50302">
        <property type="protein sequence ID" value="CAI50302"/>
        <property type="gene ID" value="NP_4422A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_4422A"/>
    <dbReference type="eggNOG" id="arCOG07988">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_218208_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="31" mass="3575" checksum="CC8B455EB99F4AF8" modified="2017-05-10" version="1">MTWADLFERADQYDVSTDAVTNALDARRNDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYK7</accession>
    <name>A0A1U7EYK7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Isocitrate lyase</fullName>
            <ecNumber evidence="2">4.1.3.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">aceA</name>
        <name evidence="2" type="ordered locus">NP_4432A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.1.3.1" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323922.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYK7"/>
    <dbReference type="STRING" id="348780.NP_4432A"/>
    <dbReference type="EnsemblBacteria" id="CAI50307">
        <property type="protein sequence ID" value="CAI50307"/>
        <property type="gene ID" value="NP_4432A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703058"/>
    <dbReference type="KEGG" id="nph:NP_4432A"/>
    <dbReference type="eggNOG" id="arCOG00582">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027389_3_2_2"/>
    <dbReference type="OrthoDB" id="9667at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004451">
        <property type="term" value="F:isocitrate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00377">
        <property type="entry name" value="ICL_PEPM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039556">
        <property type="entry name" value="ICL/PEPM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42905:SF5">
        <property type="entry name" value="CARBOXYVINYL-CARBOXYPHOSPHONATE PHOSPHORYLMUTASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42905">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13714">
        <property type="entry name" value="PEP_mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
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            <begin position="327"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI50307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="354" mass="39696" checksum="A6C73D955311C612" modified="2017-05-10" version="1">MAHDNDGTDEVASRVQNTESIVRDVDNPAARELREKFEEQDFTFAPGLYHALDARLAEMAGLDAAYMSGYSTVLGQFGFPDLEMVTMTEMVENAKRIVEATNLPVVADCDTGYGGIHNVRRAVREYEKAGVAAVHIEDQTTPKRCGHIAGKKIISREDAEARFSAAVDAKQSEDTIIIARTDAYGSANGDWEEHLERGRIYADAGVDLVWPEMPDPSREDAVEYAETIHETHPELDLAFNYSSSFAWSEEEDALTFQELGDLGYQYIFITLYALHSGAHAVYEDMKNIEQNDEEAQWDLEGRYLGHETESHHELSFVSRFQDIEAQFDPEAKERMEKSAGFTEEESDPLTSDGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYQ3</accession>
    <name>A0A1U7EYQ3_NATPD</name>
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        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
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        </recommendedName>
    </protein>
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        <name evidence="11" type="ordered locus">NP_4606A</name>
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        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
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            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
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    </organism>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
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        </reaction>
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        <property type="protein sequence ID" value="CAI50394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
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        <property type="gene ID" value="NP_4606A"/>
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    <dbReference type="GeneID" id="3702113"/>
    <dbReference type="KEGG" id="nph:NP_4606A"/>
    <dbReference type="eggNOG" id="arCOG02333">
        <property type="taxonomic scope" value="Archaea"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_71_2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004673">
        <property type="term" value="F:protein histidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="159"/>
            <end position="231"/>
        </location>
    </feature>
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            <begin position="234"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="9">
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            <begin position="283"/>
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        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="10">
        <location>
            <begin position="354"/>
            <end position="408"/>
        </location>
    </feature>
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        <location>
            <begin position="485"/>
            <end position="538"/>
        </location>
    </feature>
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            <begin position="555"/>
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        </location>
    </feature>
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    </feature>
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        <location>
            <begin position="664"/>
            <end position="723"/>
        </location>
    </feature>
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            <begin position="801"/>
            <end position="1008"/>
        </location>
    </feature>
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        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
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            <begin position="131"/>
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        </location>
    </feature>
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        <location>
            <begin position="392"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
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            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI50394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="1008" mass="113461" checksum="6A2C3CC61FBA7C09" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYS3</accession>
    <name>A0A1U7EYS3_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sulfoxide reductase heme-binding subunit YedZ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4588A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <lineage>
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            <taxon>Methanobacteriati</taxon>
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            <taxon>Stenosarchaea group</taxon>
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            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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        <property type="gene ID" value="NP_4588A"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <begin position="36"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <begin position="77"/>
            <end position="95"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="213" mass="23746" checksum="7977E01099255BDB" modified="2017-05-10" version="1">MSADTNNELTHHLAVGIGSVGVAVLLWVVGYHEQRVVGAIPWFLLVLVLAIGPAVKLWPGIRRRGSGNFPVSWRSELGIWFAIWSVVHLLFVFHARDWDVIGYLAGMSPWAFGSFVAVIMAVVLAATSNNRAYDYMGAKAWKWHQTAGTYAIFWLLAVHIYDRAYLRPGFPSEDPLHWLYLLTLGLVVVLQIAAFVKVVSHYRKTGEYPAGLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYT2</accession>
    <name>A0A1U7EYT2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF217 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4664A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324037.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYT2"/>
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            <dbReference type="ARBA" id="ARBA00022649"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI50423.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="75" mass="8725" checksum="2C497BB0D9A3AF17" modified="2017-05-10" version="1">MSTSIRLSEEAKSRLDLYKREGESYDDVIIRLTSDDKWAGFGVASSDPKESREGMEAIRDRMRSRMDDHVEKLDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYU4</accession>
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        <name evidence="4" type="ordered locus">NP_4634A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
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            <dbReference type="PubMed" id="16169924"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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    <dbReference type="EnsemblBacteria" id="CAI50408">
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        <property type="gene ID" value="NP_4634A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702126"/>
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    <dbReference type="eggNOG" id="arCOG00681">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050175_0_0_2"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
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    </dbReference>
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    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
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        <location>
            <begin position="302"/>
            <end position="364"/>
        </location>
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        <location>
            <begin position="394"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="423"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="434" mass="50266" checksum="F6D182B6565C2ACB" modified="2017-05-10" version="1">MTQTQALTKTLVFPLDVQSGNESLLHDARLECRRVFNEVLRLNYDGWNWDKIENVVEQNADLVQNTAQRIIDKAFDALDNYYDYDDWGRPWYKHETFPLRMNYGEGYNLFLEDETVRFRISAKPYNHVKGELRGTQDQFDLLRQAIEDDDWHVGTAEALVRHGREELHATVTNETAEVGAKTTTETVTGVDINEDCVALAALTEHGIEDSVVIDYPEIKEQRHRYFTMRKRMQEAGQTGFNDVFRDKEQQFVHDQLHTVSRRVVEWIQRFDSPVIVFEDLKDMRDDIEYGTRMNRRLHSLPFAKLRDFITYKAAWRGIPSDDVDPEYTSQQCPVCGHTERANRHKKRFKCCECEHQDHADRGAGISVAQKWLRTQEDRNVPALNTLPQVRKWELRRQASGPVDGPTVTHHTERGHQTDGVSGVSDQSTGRSSGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYW1</accession>
    <name>A0A1U7EYW1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ordered locus">NP_4690A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
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            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
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            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
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            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50436.1"/>
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    </dbReference>
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    <dbReference type="EnsemblBacteria" id="CAI50436">
        <property type="protein sequence ID" value="CAI50436"/>
        <property type="gene ID" value="NP_4690A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702144"/>
    <dbReference type="KEGG" id="nph:NP_4690A"/>
    <dbReference type="eggNOG" id="arCOG03730">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_304552_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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            <begin position="66"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
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            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
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            <end position="214"/>
        </location>
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        <location>
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            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
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            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="350"/>
            <end position="368"/>
        </location>
    </feature>
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        <location>
            <begin position="444"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="470"/>
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        </location>
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        <location>
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            <end position="698"/>
        </location>
    </feature>
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        <location>
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            <end position="732"/>
        </location>
    </feature>
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        <location>
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            <end position="921"/>
        </location>
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        <location>
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        </location>
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        <location>
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            <end position="886"/>
        </location>
    </feature>
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        <location>
            <begin position="844"/>
            <end position="863"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="975" mass="104782" checksum="70CB1372219F36E5" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYW3</accession>
    <name>A0A1U7EYW3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4740A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50461.1"/>
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    </dbReference>
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    </dbReference>
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    <dbReference type="STRING" id="348780.NP_4740A"/>
    <dbReference type="EnsemblBacteria" id="CAI50461">
        <property type="protein sequence ID" value="CAI50461"/>
        <property type="gene ID" value="NP_4740A"/>
    </dbReference>
    <dbReference type="GeneID" id="54763329"/>
    <dbReference type="KEGG" id="nph:NP_4740A"/>
    <dbReference type="eggNOG" id="arCOG06442">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_204976_0_0_2"/>
    <dbReference type="OrthoDB" id="295956at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="46" mass="5458" checksum="7DF0047173A9D5FE" modified="2017-05-10" version="1">MRTDVDMERDGDLHERVKEYARENGIRHPRAYRELIEKGLEATDAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYY5</accession>
    <name>A0A1U7EYY5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type transport system permease protein (Probable substrate zinc/manganese/metal ions)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">znuB2</name>
        <name evidence="7" type="synonym">abc20p</name>
        <name evidence="7" type="ordered locus">NP_4732A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC-3 integral membrane protein family.</text>
    </comment>
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        <property type="protein sequence ID" value="CAI50457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYY5"/>
    <dbReference type="STRING" id="348780.NP_4732A"/>
    <dbReference type="EnsemblBacteria" id="CAI50457">
        <property type="protein sequence ID" value="CAI50457"/>
        <property type="gene ID" value="NP_4732A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702427"/>
    <dbReference type="KEGG" id="nph:NP_4732A"/>
    <dbReference type="eggNOG" id="arCOG01006">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028808_3_1_2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06550">
        <property type="entry name" value="TM_ABC_iron-siderophores_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="entry name" value="ABC transporter involved in vitamin B12 uptake, BtuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001626">
        <property type="entry name" value="ABC_TroCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30477">
        <property type="entry name" value="ABC-TRANSPORTER METAL-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30477:SF0">
        <property type="entry name" value="METAL TRANSPORT SYSTEM MEMBRANE PROTEIN TM_0125-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00950">
        <property type="entry name" value="ABC-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="ABC transporter involved in vitamin B12 uptake, BtuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="116"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="187"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="225"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="249"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="297"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI50457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="317" mass="33784" checksum="903A830EADD51E9E" modified="2017-05-10" version="1">MAVGNIRNREYSTRALSIPVLLVAFAVFIVWLFPPLFERFWGVMEVIFNDHWLVSSSFLQHGFTAGVLIGFTAPVVGSYLVNRQMALIGEALAHTAFGGVAIGLFVGAAVPDLNYPLVWALVVATIAALGMQYVAVKTDGYADIPIAIMLTGGFAVGIAVISYGVVFSATVESYLFGDILFVPFENVQLMVGLTLLVGLVVGLTHKQLKFITFDREAARLARINVWFYDTLLIVLTALVVVAAMQILGAILVAGMLVIPVAAAMQVTGSFNRGMLMSVGFGQVAVIGGILSSYYLGIATGPMIIIVAILIYIGAVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYZ0</accession>
    <name>A0A1U7EYZ0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC-type transport system periplasmic substrate-binding protein (Probable substrate zinc/manganese/metal ions)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">znuA2</name>
        <name evidence="5" type="synonym">abc20s</name>
        <name evidence="5" type="ordered locus">NP_4728A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial solute-binding protein 9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324068.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYZ0"/>
    <dbReference type="STRING" id="348780.NP_4728A"/>
    <dbReference type="EnsemblBacteria" id="CAI50455">
        <property type="protein sequence ID" value="CAI50455"/>
        <property type="gene ID" value="NP_4728A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702428"/>
    <dbReference type="KEGG" id="nph:NP_4728A"/>
    <dbReference type="eggNOG" id="arCOG01005">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016838_1_1_2"/>
    <dbReference type="OrthoDB" id="50488at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
        <property type="term" value="P:metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1980">
        <property type="entry name" value="Nitrogenase molybdenum iron protein domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050492">
        <property type="entry name" value="Bact_metal-bind_prot9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006127">
        <property type="entry name" value="ZnuA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42953:SF3">
        <property type="entry name" value="HIGH-AFFINITY ZINC UPTAKE SYSTEM PROTEIN ZNUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42953">
        <property type="entry name" value="HIGH-AFFINITY ZINC UPTAKE SYSTEM PROTEIN ZNUA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01297">
        <property type="entry name" value="ZnuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53807">
        <property type="entry name" value="Helical backbone' metal receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="30"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI50455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="340" mass="36947" checksum="878ADB205206A0F4" modified="2017-05-10" version="1">MSSHPRQNESKGVSRRTFALLAGSAVTAGLAGCTDDPMENGDAPATNGGDDPSDLDVDDRTVVASMPALWDFTRQVAGDGDDTVQPIDIVPVGEHGHDWNPEPATVEDVDHAGAFVYMRDFAAWQDDAAAALEDDGGTVVIDASAGIDFFDSPAEDNDEHWWMDPVYAQEGVSNIADGLAEMDPDNADYYRDNAAAFTDELQALHEDFQDIVDRAELTSIVVATHDSFQWWNRQYGIDVHSPVGTSPDDAATPQDVQEIEELMEDEGIEHVLYDVGEPAQLAESLAAETDADILPLTPVETQIDGAPELDTGIEMEPDWGYLEHFYEINLPSLETALHAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYZ3</accession>
    <name>A0A1U7EYZ3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Molybdopterin adenylyltransferase</fullName>
            <ecNumber evidence="5">2.7.7.75</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">moaB1</name>
        <name evidence="5" type="ordered locus">NP_4814A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MoaB/Mog family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.75" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324110.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYZ3"/>
    <dbReference type="STRING" id="348780.NP_4814A"/>
    <dbReference type="EnsemblBacteria" id="CAI50498">
        <property type="protein sequence ID" value="CAI50498"/>
        <property type="gene ID" value="NP_4814A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702923"/>
    <dbReference type="KEGG" id="nph:NP_4814A"/>
    <dbReference type="eggNOG" id="arCOG00214">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077358_2_1_2"/>
    <dbReference type="OrthoDB" id="205337at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061598">
        <property type="term" value="F:molybdopterin adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00886">
        <property type="entry name" value="MogA_MoaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.980.10:FF:000006">
        <property type="entry name" value="Molybdenum cofactor biosynthesis protein B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012245">
        <property type="entry name" value="MoaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008284">
        <property type="entry name" value="MoCF_biosynth_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43232">
        <property type="entry name" value="MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43232:SF2">
        <property type="entry name" value="MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01078">
        <property type="entry name" value="MOCF_BIOSYNTHESIS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MoaB/Mog" evidence="4">
        <location>
            <begin position="58"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="25"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI50498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="208" mass="21987" checksum="460506A8EF5B8E13" modified="2017-05-10" version="1">MTDDTDTEHGDEGAVDEDNGTGGADETHRDHDGDDGHDHDDGHDHDHHHHDVDSLGVGLLTISSSRTIEEDGAGDAIAEAMADAGHEIAVRELVPDDYDRVQDTVDRFIGRDDVDCVVTTGGTGVTPDDVTIEAVEPLFDKRLPGFGELFRTLSYEEIDTRVVGTRAVGGIADGAAVFCLPGSENAAQLGTEEIIVAEAPHLVGLAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ60</accession>
    <name>A0A1U7EZ60_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphoribosylaminoimidazole-succinocarboxamide synthase</fullName>
            <ecNumber evidence="6">6.3.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">SAICAR synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">purC</name>
        <name evidence="8" type="ordered locus">NP_4996A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate + ATP = (2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:22628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SAICAR synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.6" evidence="6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324200.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ60"/>
    <dbReference type="STRING" id="348780.NP_4996A"/>
    <dbReference type="EnsemblBacteria" id="CAI50589">
        <property type="protein sequence ID" value="CAI50589"/>
        <property type="gene ID" value="NP_4996A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703068"/>
    <dbReference type="KEGG" id="nph:NP_4996A"/>
    <dbReference type="eggNOG" id="arCOG04421">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045637_1_0_2"/>
    <dbReference type="OrthoDB" id="10775at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00131"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004639">
        <property type="term" value="F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00137">
        <property type="entry name" value="SAICAR_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028923">
        <property type="entry name" value="SAICAR_synt/ADE2_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010566">
        <property type="entry name" value="PRK13959.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43700">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE-SUCCINOCARBOXAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43700:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE-SUCCINOCARBOXAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01259">
        <property type="entry name" value="SAICAR_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 6 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 6" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAICAR synthetase/ADE2 N-terminal" evidence="7">
        <location>
            <begin position="22"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI50589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="339" mass="37784" checksum="7262CAC11885368A" modified="2017-05-10" version="1">MTSVKEFRIEEPATETDLGAGAFVFTDDYSVFDWGKMPDSIPNKGASLCAMGAANFERLEAEGIPTHYRGVVSGGEVVALSEAVSPPREMAIELTQVPDLPHDGREYDYEAFHADAGENYLIPLEIVFRNTVPVGSSLRSRADPEAFGLDYEEWPDEPVDLPEPVVEFSTKFEESDRYLSRSNADYIAGKADIDDLESVARDVNTIVTETAAEAGLKHEDGKIECLYFDGEIRVADVVGTFDENRFSHDGQQLSKEVVRQYHKRTQSEWVEAVGEAKERAKAEDVADWRELCETEPEPLDDHVIDAVRDMYTAGTNAYIDRELFSAPPLSEAVETVRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ64</accession>
    <name>A0A1U7EZ64_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphoribosylformylglycinamidine synthase subunit PurS</fullName>
            <shortName evidence="6">FGAM synthase</shortName>
            <ecNumber evidence="6">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Formylglycinamide ribonucleotide amidotransferase subunit III</fullName>
            <shortName evidence="6">FGAR amidotransferase III</shortName>
            <shortName evidence="6">FGAR-AT III</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Phosphoribosylformylglycinamidine synthase subunit III</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">purS</name>
        <name evidence="7" type="ordered locus">NP_5008A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + L-glutamine + ATP + H2O = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PurS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324205.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ64"/>
    <dbReference type="STRING" id="348780.NP_5008A"/>
    <dbReference type="EnsemblBacteria" id="CAI50595">
        <property type="protein sequence ID" value="CAI50595"/>
        <property type="gene ID" value="NP_5008A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703078"/>
    <dbReference type="KEGG" id="nph:NP_5008A"/>
    <dbReference type="eggNOG" id="arCOG04462">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_164833_3_0_2"/>
    <dbReference type="OrthoDB" id="56303at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1280.10">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase subunit PurS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01926">
        <property type="entry name" value="PurS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003850">
        <property type="entry name" value="PurS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036604">
        <property type="entry name" value="PurS-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00302">
        <property type="entry name" value="phosphoribosylformylglycinamidine synthase subunit PurS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004630">
        <property type="entry name" value="PRK05974.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34696">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34696:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02700">
        <property type="entry name" value="PurS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82697">
        <property type="entry name" value="PurS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 6" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI50595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="83" mass="9315" checksum="EE48353E955A560B" modified="2017-05-10" version="1">MTAYTATVTVRLKRGVLDPEAETTQQALERLGFELEDLRSSDRFEVDLDADDAESARERATEMAERLLANPTIHDYDVDVAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ80</accession>
    <name>A0A1U7EZ80_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4998A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324201.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ80"/>
    <dbReference type="STRING" id="348780.NP_4998A"/>
    <dbReference type="EnsemblBacteria" id="CAI50590">
        <property type="protein sequence ID" value="CAI50590"/>
        <property type="gene ID" value="NP_4998A"/>
    </dbReference>
    <dbReference type="GeneID" id="43500104"/>
    <dbReference type="KEGG" id="nph:NP_4998A"/>
    <dbReference type="HOGENOM" id="CLU_3075449_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="52" mass="5632" checksum="910DE21062E8A4C5" modified="2017-05-10" version="1">MTPLQILDPVMAVVLLGIVTTLAIVFGFIWAYGKIREPPEIHTDDENGGHAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZB8</accession>
    <name>A0A1U7EZB8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">IS1341-type transposase ISNph16</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_5060A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
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            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAI50621"/>
        <property type="gene ID" value="NP_5060A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702243"/>
    <dbReference type="KEGG" id="nph:NP_5060A"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_3_3_2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
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        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
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        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNA-GUIDED DNA ENDONUCLEASE RV2885C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="298"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="392"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="403"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="425" mass="47830" checksum="F15F30966C1907BF" modified="2017-05-10" version="1">MEVRRTVPVKLDLDSDDAALLRETVGEFLWAANYVVDHAWQGEYKTTSKAQLQEETYDEVREQTRLHANLVQNARNKAADAVQGAVARWKQGEYAGKPHFSQPTVVYDKRCATFHDESVSLATVDGRIEVEYVLPDGDRDTPHSRYLDNDDYEVTGAELHNKDGEWFLHLRTKAEMESDTPEQATTGHSTVLGVDLGVNQLAVTSTGRFWSGHEFDHWRREYEKRRASLQQCGSRHAHENIQSIGRTETGRFKMMLHRIANGIIEEAVENDCTVIAFEELTGIRDRLSGASWGHKWAFERLYEYVEYKSELYGIDVEQVDPENTSRRCSHCGFTHPDNRDGEGFECLKCGYENHADYNAAKNIGLRYLRRNQTGDGGGAPVGVRLNRGMVNANGVYEPPAGESGQSGSPRESPTLNEANGEAVSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZD6</accession>
    <name>A0A1U7EZD6_NATPD</name>
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        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">NP_5120A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324261.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZD6"/>
    <dbReference type="STRING" id="348780.NP_5120A"/>
    <dbReference type="EnsemblBacteria" id="CAI50651">
        <property type="protein sequence ID" value="CAI50651"/>
        <property type="gene ID" value="NP_5120A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702263"/>
    <dbReference type="KEGG" id="nph:NP_5120A"/>
    <dbReference type="eggNOG" id="arCOG02369">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02388">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_58_2"/>
    <dbReference type="OrthoDB" id="8127at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00075">
        <property type="entry name" value="HATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00156">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050736">
        <property type="entry name" value="Sensor_HK_Regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43711:SF1">
        <property type="entry name" value="HISTIDINE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43711">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="11">
        <location>
            <begin position="32"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="12">
        <location>
            <begin position="158"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="13">
        <location>
            <begin position="231"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="10">
        <location>
            <begin position="459"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="267"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAI50651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="649" mass="70081" checksum="EC6297C6516B6F1D" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZD9</accession>
    <name>A0A1U7EZD9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5108A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324255.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZD9"/>
    <dbReference type="STRING" id="348780.NP_5108A"/>
    <dbReference type="EnsemblBacteria" id="CAI50645">
        <property type="protein sequence ID" value="CAI50645"/>
        <property type="gene ID" value="NP_5108A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702258"/>
    <dbReference type="KEGG" id="nph:NP_5108A"/>
    <dbReference type="eggNOG" id="arCOG06348">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123711_0_0_2"/>
    <dbReference type="OrthoDB" id="252552at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058370">
        <property type="entry name" value="DUF8057"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26244">
        <property type="entry name" value="DUF8057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="130" mass="14287" checksum="94AC1116FBB486D3" modified="2017-05-10" version="1">MYESYFDDDVDYTSEAVLERAYALGVASVCDTPGSEEYERLKANSPDAYDESILELAYEEGRARALSLEADDEPDEEIWERLVEQELGDISGDDDAGTDTPADGLPEALSKSPLSDGPPESLSLPSFLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZH4</accession>
    <name>A0A1U7EZH4_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Putative [LysW]-aminoadipate/[LysW]-glutamate kinase</fullName>
            <ecNumber evidence="8">2.7.2.17</ecNumber>
            <ecNumber evidence="8">2.7.2.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">argB</name>
        <name evidence="8" type="synonym">lysZ</name>
        <name evidence="10" type="ordered locus">NP_5262A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in both the arginine and lysine biosynthetic pathways. Phosphorylates the LysW-bound precursors glutamate (for arginine biosynthesis), respectively alpha-aminoadipate (for lysine biosynthesis).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>[amino-group carrier protein]-C-terminal-N-(1,4-dicarboxybutan-1-yl)-L-glutamine + ATP = [amino-group carrier protein]-C-terminal-N-(1-carboxy-5-phosphooxy-5-oxopentan-1-yl)-L-glutamine + ADP</text>
            <dbReference type="Rhea" id="RHEA:41944"/>
            <dbReference type="Rhea" id="RHEA-COMP:9694"/>
            <dbReference type="Rhea" id="RHEA-COMP:9712"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:78499"/>
            <dbReference type="ChEBI" id="CHEBI:78503"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>[amino-group carrier protein]-C-terminal-gamma-(L-glutamyl)-L-glutamate + ATP = [amino-group carrier protein]-C-terminal-gamma-(5-phospho-L-glutamyl)-L-glutamate + ADP</text>
            <dbReference type="Rhea" id="RHEA:52632"/>
            <dbReference type="Rhea" id="RHEA-COMP:13311"/>
            <dbReference type="Rhea" id="RHEA-COMP:13313"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:136714"/>
            <dbReference type="ChEBI" id="CHEBI:136717"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis; L-arginine biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (Thermus route): step 2/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the acetylglutamate kinase family. LysZ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.17" evidence="8"/>
    <dbReference type="EC" id="2.7.2.19" evidence="8"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324331.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZH4"/>
    <dbReference type="STRING" id="348780.NP_5262A"/>
    <dbReference type="EnsemblBacteria" id="CAI50722">
        <property type="protein sequence ID" value="CAI50722"/>
        <property type="gene ID" value="NP_5262A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702516"/>
    <dbReference type="KEGG" id="nph:NP_5262A"/>
    <dbReference type="eggNOG" id="arCOG00862">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053680_2_1_2"/>
    <dbReference type="OrthoDB" id="6816at2157"/>
    <dbReference type="UniPathway" id="UPA00033">
        <property type="reaction ID" value="UER00036"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00068"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003991">
        <property type="term" value="F:acetylglutamate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043744">
        <property type="term" value="F:N2-acetyl-L-aminoadipate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02082">
        <property type="entry name" value="LysZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004662">
        <property type="entry name" value="AcgluKinase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001057">
        <property type="entry name" value="Glu/AcGlu_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037529">
        <property type="entry name" value="LysZ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00761">
        <property type="entry name" value="argB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010659">
        <property type="entry name" value="PRK14058.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010661">
        <property type="entry name" value="PRK14058.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23342:SF20">
        <property type="entry name" value="[LYSW]-AMINOADIPATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23342">
        <property type="entry name" value="N-ACETYLGLUTAMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000728">
        <property type="entry name" value="NAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00474">
        <property type="entry name" value="GLU5KINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 8" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aspartate/glutamate/uridylate kinase" evidence="9">
        <location>
            <begin position="7"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="8">
        <location>
            <position position="10"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="8">
        <location>
            <position position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI50722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="277" mass="28463" checksum="1A0423DD0EB37323" modified="2017-05-10" version="1">MTAETPVVVKIGGARAVDPEGALSDIASLVEDGTEIVVVHGGSTAVDNTLERMDIDPEYVETPSGVVGRFTDAETMDVFKMAMAGQVNTDLVVGLQNQGVDAVGLSGADGKLLYGPRKSAVRVVEDGKKKIRRGDHSGRIKEVNAGLLETLLADGYTPVASPPMLADDGVAVNTDADRASAAVAGAVGGTLVSLTDVEGVYRDPDDPATLIERVETPDDYDELDAAAEGFMSRKVMAATEALESGADEVVIADANADAPVSTAVDGGGTHILPSALE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZM7</accession>
    <name>A0A1U7EZM7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Agmatinase</fullName>
            <ecNumber evidence="6">3.5.3.11</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">speB2</name>
        <name evidence="6" type="synonym">speD2</name>
        <name evidence="6" type="ordered locus">NP_4754A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="4">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the arginase family. Agmatinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.3.11" evidence="6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CCI69574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939710.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZM7"/>
    <dbReference type="STRING" id="348780.NP_4754A"/>
    <dbReference type="EnsemblBacteria" id="CCI69574">
        <property type="protein sequence ID" value="CCI69574"/>
        <property type="gene ID" value="NP_4754A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703213"/>
    <dbReference type="KEGG" id="nph:NP_4754A"/>
    <dbReference type="eggNOG" id="arCOG01700">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039478_0_2_2"/>
    <dbReference type="OrthoDB" id="7186at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008783">
        <property type="term" value="F:agmatinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033389">
        <property type="term" value="P:putrescine biosynthetic process from arginine, via agmatine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.10">
        <property type="entry name" value="Ureohydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020855">
        <property type="entry name" value="Ureohydrolase_Mn_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358">
        <property type="entry name" value="ARGINASE/AGMATINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358:SF26">
        <property type="entry name" value="GUANIDINO ACID HYDROLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036979">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01053">
        <property type="entry name" value="ARGINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR036979-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCI69574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="266" mass="28388" checksum="86EC944E8C3904D1" modified="2017-05-10" version="1">MGGFPGANASREAADYAVVGAPLDVSTTFQPGTRFGPDQVRRFARPFDDYDHRTGRRFSDLVCDAGDVAAWDDAADYLQFLDGQLTDHHRDGRVPLLLGGEHTVTVAGLRAADPDVYVCLDAHLDLYESYAGNDLAHATVAHHALDIADEAILLGVRTGSEAEWDRAAESDVTVVEPEAVAGWTPDFDGEAYFSVDIDAADPAYAPGTGTPVPFGLEPRTMRSVVRAVAPQAVGFDVVEVNDRDDGQAASLAGKLLQEFVFAHADG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILT6</accession>
    <name>Q3ILT6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3312A</name>
        <name evidence="3" type="ordered locus">NP_7036A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>PL23</name>
    </geneLocation>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
            <strain evidence="3">Gabara</strain>
            <plasmid evidence="3">PL23</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CR936259">
        <property type="protein sequence ID" value="CAI50934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323368.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILT6"/>
    <dbReference type="STRING" id="348780.NP_3312A"/>
    <dbReference type="EnsemblBacteria" id="CAI49747">
        <property type="protein sequence ID" value="CAI49747"/>
        <property type="gene ID" value="NP_3312A"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI50934">
        <property type="protein sequence ID" value="CAI50934"/>
        <property type="gene ID" value="NP_7036A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703478"/>
    <dbReference type="KEGG" id="nph:NP_3312A"/>
    <dbReference type="KEGG" id="nph:NP_7036A"/>
    <dbReference type="eggNOG" id="arCOG02544">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_481146_0_0_2"/>
    <dbReference type="OrthoDB" id="340654at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="499"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="531"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="566" mass="60157" checksum="F48A01C24731EB02" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILZ3</accession>
    <name>Q3ILZ3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC-type transport system permease protein (Probable substrate branched-chain amino acids)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">livH2</name>
        <name evidence="10" type="synonym">abc06p1</name>
        <name evidence="10" type="ordered locus">NP_6192A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10 11">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324474.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILZ3"/>
    <dbReference type="EnsemblBacteria" id="CAI50876">
        <property type="protein sequence ID" value="CAI50876"/>
        <property type="gene ID" value="NP_6192A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694666"/>
    <dbReference type="KEGG" id="nph:NP_6192A"/>
    <dbReference type="HOGENOM" id="CLU_039929_2_0_2"/>
    <dbReference type="OrthoDB" id="43815at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06582">
        <property type="entry name" value="TM_PBP1_LivH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052157">
        <property type="entry name" value="BCAA_transport_permease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11795:SF445">
        <property type="entry name" value="AMINO ACID ABC TRANSPORTER PERMEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11795">
        <property type="entry name" value="BRANCHED-CHAIN AMINO ACID TRANSPORT SYSTEM PERMEASE PROTEIN LIVH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="52"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="227"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI50876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="294" mass="31008" checksum="DAF2D4E74FB76ECA" modified="2005-11-08" version="1">MDLLIQTIINGLLLGGIYISVGIGFALVFGVLEVIDFAVGEYVMVGAFAGSILAVTVGVDALFLAPVIFVLFYIVGFFVQPFLHHVTTSDVPMPVLMALVFTFGIAFFARGGVLTTFGPNTREVPTEIATGGITLSSIGTIPTARLITGIFGVVAVSAFLYYLYNTDGGLAIRAIAEDRTDARLMGINIARYQSVAYGMYAGLTATAGLFIGIVFSADPGMGMQYTAFAFFMVVLAGMGYLPGIIVSGLLLGLAQTMFSTYVSGAWVFFVLFGIVYVVLLVSPQGILGRGEPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM01</accession>
    <name>Q3IM01_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">IclR family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_6176A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5 6">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324466.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM01"/>
    <dbReference type="EnsemblBacteria" id="CAI50868">
        <property type="protein sequence ID" value="CAI50868"/>
        <property type="gene ID" value="NP_6176A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694636"/>
    <dbReference type="KEGG" id="nph:NP_6176A"/>
    <dbReference type="HOGENOM" id="CLU_062618_6_1_2"/>
    <dbReference type="OrthoDB" id="14763at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050707">
        <property type="entry name" value="HTH_MetabolicPath_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014757">
        <property type="entry name" value="Tscrpt_reg_IclR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005471">
        <property type="entry name" value="Tscrpt_reg_IclR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30136">
        <property type="entry name" value="HELIX-TURN-HELIX TRANSCRIPTIONAL REGULATOR, ICLR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30136:SF35">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR RV1719"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01614">
        <property type="entry name" value="IclR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00346">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51078">
        <property type="entry name" value="ICLR_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="IclR-ED" evidence="4">
        <location>
            <begin position="71"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI50868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="256" mass="28390" checksum="BCF7687304286636" modified="2005-11-08" version="1">MVSEPDNDGGIKSAGTSFGIIETIYQNQPISLTELAAEVDIANSTAYEHISTLLDSGYIVKRADGYYLSLKFLDRGTKAKEYYQELLDVSEPALEQLVDETDEAVNLVVEEHGQAVYIDRRTGERGVPTNSWVGKRKPLHTLSAGKAILSQLPEERVDEILDARGLSVSTERAITTREELYDELEEIRQNGVSFNDRESHSQIRAVGAPIVVDDRVYGAVSVAGPAMRLTGEYFRQEIADLLQGAVNEIELKLTYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM08</accession>
    <name>Q3IM08_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HTH domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_6160A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324459.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM08"/>
    <dbReference type="EnsemblBacteria" id="CAI50860">
        <property type="protein sequence ID" value="CAI50860"/>
        <property type="gene ID" value="NP_6160A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694632"/>
    <dbReference type="KEGG" id="nph:NP_6160A"/>
    <dbReference type="HOGENOM" id="CLU_097789_1_0_2"/>
    <dbReference type="OrthoDB" id="183382at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055766">
        <property type="entry name" value="DUF7342"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24033">
        <property type="entry name" value="DUF7342"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="193" mass="22381" checksum="8CDC916667656895" modified="2005-11-08" version="1">MTSTYIKTKPERCCMTEDESDSEGLMERQTTGEDRVRMVARQLSEPQTANWIASEAGWSHEPTKRVLERLVDDGILHCDESGTHTTYYPDYRRQAMQEAMRLRDSGHTVEELTDRLADMKTQIRDWEDEFGVESPNQLRGTLADESLDGGEEDRRRDIAREWEHLQRRIQIVGFAIREWDFLAPTTEPAEASS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-02-06" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM24</accession>
    <name>PHNC2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphonates import ATP-binding protein PhnC 2</fullName>
            <ecNumber evidence="1">7.3.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">phnC2</name>
        <name type="synonym">abc18a</name>
        <name type="ordered locus">NP_6124A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>PL131</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the ABC transporter complex PhnCDE involved in phosphonates import. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>phosphonate(out) + ATP + H2O = phosphonate(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16215"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.3.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The complex is composed of two ATP-binding proteins (PhnC), two transmembrane proteins (PhnE) and a solute-binding protein (PhnD).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. Phosphonates importer (TC 3.A.1.9.1) family.</text>
    </comment>
    <dbReference type="EC" id="7.3.2.2" evidence="1"/>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324443.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM24"/>
    <dbReference type="SMR" id="Q3IM24"/>
    <dbReference type="EnsemblBacteria" id="CAI50842">
        <property type="protein sequence ID" value="CAI50842"/>
        <property type="gene ID" value="NP_6124A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694675"/>
    <dbReference type="KEGG" id="nph:NP_6124A"/>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="OrthoDB" id="302885at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015416">
        <property type="term" value="F:ABC-type phosphonate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03256">
        <property type="entry name" value="ABC_PhnC_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012693">
        <property type="entry name" value="ABC_transpr_PhnC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050086">
        <property type="entry name" value="MetN_ABC_transporter-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02315">
        <property type="entry name" value="ABC_phnC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43166">
        <property type="entry name" value="AMINO ACID IMPORT ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43166:SF6">
        <property type="entry name" value="PHOSPHONATES IMPORT ATP-BINDING PROTEIN PHNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51249">
        <property type="entry name" value="PHNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0918">Phosphonate transport</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000274774" description="Phosphonates import ATP-binding protein PhnC 2">
        <location>
            <begin position="1"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="1">
        <location>
            <begin position="2"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="243"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="267" mass="29432" checksum="FF3613D1AE3D8944" modified="2005-11-08" version="1">MLRVESLRKVYDSGDVALDDVSLTVSGSETVAIIGPSGAGKSTLVRCINRLVEPTSGTVWLDEREVTGLPEKQLPAVRRDMGMIFQEYNLIERLTVMENVLSGRLGYVSSWKGFRRKFPQEDVDRAFEILDRVGLSGLENNRADELSGGQRQRVGIARAVIQQPKIMLVDEPTSSLDPDTSRDVMDLLTEIASDEGVPVLLNIHEVDLAVEYADRIVGLADGRIVYDGSTDGLDEGIENKIYRNGRESARSSPSSEPALSDDTELHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM59</accession>
    <name>Q3IM59_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Homolog to restriction system mrr</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_6044A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324408.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM59"/>
    <dbReference type="EnsemblBacteria" id="CAI50802">
        <property type="protein sequence ID" value="CAI50802"/>
        <property type="gene ID" value="NP_6044A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694690"/>
    <dbReference type="KEGG" id="nph:NP_6044A"/>
    <dbReference type="HOGENOM" id="CLU_606365_0_0_2"/>
    <dbReference type="OrthoDB" id="144520at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015666">
        <property type="term" value="F:restriction endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007560">
        <property type="entry name" value="Restrct_endonuc_IV_Mrr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052906">
        <property type="entry name" value="Type_IV_Methyl-Rstrct_Enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30015">
        <property type="entry name" value="MRR RESTRICTION SYSTEM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30015:SF7">
        <property type="entry name" value="TYPE IV METHYL-DIRECTED RESTRICTION ENZYME ECOKMRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04471">
        <property type="entry name" value="Mrr_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Restriction endonuclease type IV Mrr" evidence="1">
        <location>
            <begin position="4"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="460" mass="51489" checksum="C32523685549509F" modified="2005-11-08" version="1">MPILDEVDGFEFEEVMCSVFRNLGYEDVEKSRKTADKGRDITMIDSASRGKDEAVVVECKHTDTVGRPVVQKLDSAVSTYSHDGPKRGMIATTGRLTNPAIEYAEQVGIEIFDGQRIREVADEVGMDLYNGRVEIITDSVLHPPRPQHVLDEFYAVIDRIKYLSRDEVPDPLTTISLHPVIAADTKTNRVFETGAGVIHTIDETDNIVVDGMSSELDGLTDDLDTIVSAYDLDTDVKVIDEAEYTQVADRIDRRRFGGTDQEFRRWIKQQERDRLTETVSYTGDNNQTYSKECAPSLDDVTIRLLRPFYVCRIDASMKLNKRTYALDWFATADDSWVVDNTIETCRFAEEGRGSIDSVPAAATALKEAIGDPTYAFCPLCETIVAERYMRHDGVTGEPVCVECAVTDRFVGAKKHFRSDDTRQQYEDEVAAKGTVEKLSVNKTGVITVLAVLLVFALLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM64</accession>
    <name>Q3IM64_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">3-oxoacid CoA-transferase beta subunit</fullName>
            <ecNumber evidence="1">2.8.3.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_6032A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.8.3.-" evidence="1"/>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324403.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM64"/>
    <dbReference type="EnsemblBacteria" id="CAI50796">
        <property type="protein sequence ID" value="CAI50796"/>
        <property type="gene ID" value="NP_6032A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694590"/>
    <dbReference type="KEGG" id="nph:NP_6032A"/>
    <dbReference type="HOGENOM" id="CLU_069088_0_0_2"/>
    <dbReference type="OrthoDB" id="9252at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008410">
        <property type="term" value="F:CoA-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1080.10">
        <property type="entry name" value="Glutaconate Coenzyme A-transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004165">
        <property type="entry name" value="CoA_trans_fam_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43293">
        <property type="entry name" value="ACETATE COA-TRANSFERASE YDIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43293:SF3">
        <property type="entry name" value="CHOLESTEROL RING-CLEAVING HYDROLASE IPDB SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01144">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00882">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="252" mass="27295" checksum="14631913E9413AC2" modified="2005-11-08" version="1">MSESYSPTELLTTVASTLIEDESTIIVGTGMPMLAAMLAQKTHAPDATMIYEAGGIGPDAPELPVSVGDERTFHRGVKAAGMHEVMSYMSAGFVDYGFLGAAQIDRHGNLNSTVIGDWEHPTVRFPGSGGANDIGSLANETIVAMRQDETSFVEELDFRTTPGYLDGPGARKEAGLPAETGPSNVITQYGVYGFDDNQELTLEYLHPGVEEETVIEESGFEIHIGDYERSPEPTDKQLRLLREEIDPQGIIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM66</accession>
    <name>Q3IM66_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">NP_6028A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11 12">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324401.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM66"/>
    <dbReference type="EnsemblBacteria" id="CAI50794">
        <property type="protein sequence ID" value="CAI50794"/>
        <property type="gene ID" value="NP_6028A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694588"/>
    <dbReference type="KEGG" id="nph:NP_6028A"/>
    <dbReference type="HOGENOM" id="CLU_000445_114_58_2"/>
    <dbReference type="OrthoDB" id="8127at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00075">
        <property type="entry name" value="HATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00156">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052162">
        <property type="entry name" value="Sensor_kinase/Photoreceptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43304:SF1">
        <property type="entry name" value="PAC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43304">
        <property type="entry name" value="PHYTOCHROME-LIKE PROTEIN CPH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0614">Plasmid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Response regulatory" evidence="8">
        <location>
            <begin position="9"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="9">
        <location>
            <begin position="140"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="10">
        <location>
            <begin position="215"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="9">
        <location>
            <begin position="268"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="10">
        <location>
            <begin position="343"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="9">
        <location>
            <begin position="387"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="10">
        <location>
            <begin position="464"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="527"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="6">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI50794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="719" mass="81484" checksum="5D90538609BA1538" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IPM9</accession>
    <name>RL7A_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein eL8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L7Ae</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Ribosomal protein L8e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl7ae</name>
        <name type="ordered locus">NP_3660A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal RNA, the RNA component of RNase P, box H/ACA, box C/D and box C'/D' sRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Probably part of the RNase P complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323539.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IPM9"/>
    <dbReference type="SMR" id="Q3IPM9"/>
    <dbReference type="STRING" id="348780.NP_3660A"/>
    <dbReference type="EnsemblBacteria" id="CAI49921">
        <property type="protein sequence ID" value="CAI49921"/>
        <property type="gene ID" value="NP_3660A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703148"/>
    <dbReference type="KEGG" id="nph:NP_3660A"/>
    <dbReference type="eggNOG" id="arCOG01751">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084513_4_0_2"/>
    <dbReference type="OrthoDB" id="25810at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000020">
        <property type="entry name" value="50S ribosomal protein L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00326">
        <property type="entry name" value="Ribosomal_eL8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_eL8/Nhp2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022481">
        <property type="entry name" value="Ribosomal_eL8_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03677">
        <property type="entry name" value="eL8_ribo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00884">
        <property type="entry name" value="RIBOSOMALHS6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_1000005031" description="Large ribosomal subunit protein eL8">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="120" mass="12701" checksum="2C7BEC08D61D2097" modified="2005-11-08" version="1">MSVYVDFDVPADLEDDALEALEVARDTGSVKKGTNETTKAVERGNAELVFVAEDVSPEEVVMHLPEIATEKEIPYVFVGTQDDIGHAAGLQVGSAAAAIVDAGEANGEVEDIAEKVEELQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IPW6</accession>
    <name>TOP6B_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Type 2 DNA topoisomerase 6 subunit B</fullName>
            <ecNumber evidence="1">5.6.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Type II DNA topoisomerase VI subunit B</fullName>
            <shortName evidence="1">TopoVI-B</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">top6B</name>
        <name type="ordered locus">NP_3482A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Relaxes both positive and negative superturns and exhibits a strong decatenase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Heterotetramer of two Top6A and two Top6B chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TOP6B family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323452.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IPW6"/>
    <dbReference type="SMR" id="Q3IPW6"/>
    <dbReference type="STRING" id="348780.NP_3482A"/>
    <dbReference type="EnsemblBacteria" id="CAI49832">
        <property type="protein sequence ID" value="CAI49832"/>
        <property type="gene ID" value="NP_3482A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703276"/>
    <dbReference type="KEGG" id="nph:NP_3482A"/>
    <dbReference type="eggNOG" id="arCOG01165">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006403_0_0_2"/>
    <dbReference type="OrthoDB" id="65493at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16933">
        <property type="entry name" value="HATPase_TopVIB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00823">
        <property type="entry name" value="TopoIIB_Trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000062">
        <property type="entry name" value="Type 2 DNA topoisomerase 6 subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00322">
        <property type="entry name" value="Top6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040494">
        <property type="entry name" value="Top6b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005734">
        <property type="entry name" value="TopoVI_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015320">
        <property type="entry name" value="TopoVI_B_transducer"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003218">
        <property type="entry name" value="PRK04184.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011439">
        <property type="entry name" value="PRK14868.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01052">
        <property type="entry name" value="top6b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48444">
        <property type="entry name" value="DNA TOPOISOMERASE 6 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48444:SF1">
        <property type="entry name" value="DNA TOPOISOMERASE 6 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18000">
        <property type="entry name" value="Top6b_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09239">
        <property type="entry name" value="Topo-VIb_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0799">Topoisomerase</keyword>
    <feature type="chain" id="PRO_1000005872" description="Type 2 DNA topoisomerase 6 subunit B">
        <location>
            <begin position="1"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="221"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="221"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="112"/>
            <end position="113"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="122"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="629"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="798" mass="87363" checksum="82DF9847312DB62A" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IRJ0</accession>
    <name>TRMY_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA (pseudouridine(54)-N(1))-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.257</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trmY</name>
        <name type="ordered locus">NP_2324A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically catalyzes the N1-methylation of pseudouridine at position 54 (Psi54) in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>pseudouridine(54) in tRNA + S-adenosyl-L-methionine = N(1)-methylpseudouridine(54) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55292"/>
            <dbReference type="Rhea" id="RHEA-COMP:14140"/>
            <dbReference type="Rhea" id="RHEA-COMP:14141"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:74890"/>
            <dbReference type="EC" id="2.1.1.257"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. TrmY family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.257" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322880.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IRJ0"/>
    <dbReference type="SMR" id="Q3IRJ0"/>
    <dbReference type="STRING" id="348780.NP_2324A"/>
    <dbReference type="EnsemblBacteria" id="CAI49253">
        <property type="protein sequence ID" value="CAI49253"/>
        <property type="gene ID" value="NP_2324A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701405"/>
    <dbReference type="KEGG" id="nph:NP_2324A"/>
    <dbReference type="eggNOG" id="arCOG01239">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107018_0_0_2"/>
    <dbReference type="OrthoDB" id="27492at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18087">
        <property type="entry name" value="TrmY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00587">
        <property type="entry name" value="tRNA_methyltr_TrmY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007158">
        <property type="entry name" value="TrmY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002560">
        <property type="entry name" value="PRK02135.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40703">
        <property type="entry name" value="TRNA (PSEUDOURIDINE(54)-N(1))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40703:SF1">
        <property type="entry name" value="TRNA (PSEUDOURIDINE(54)-N(1))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04013">
        <property type="entry name" value="Methyltrn_RNA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_1000025473" description="tRNA (pseudouridine(54)-N(1))-methyltransferase">
        <location>
            <begin position="1"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00587"/>
        </source>
    </evidence>
    <sequence length="198" mass="21504" checksum="0284866553A0B82E" modified="2005-11-08" version="1">MRQFIVIGHDVPTDPDAVSLADLPGAGRLDVLCRCLNSAFFLSHDLRDNVRVWLVVGDEYAIRFEGSELRRLNPDERSTAALVRGALAAREKAIGSMAANPSPGVYIRTQSLSGLLESVADDGPLVTLHEDGQPVVDIDPSAEPTFVLSDHHSFDDAETALLAAHADRRVSLGPEPLHADHAITVAHNYLDTDGYNRY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IT14</accession>
    <name>IF1A_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Translation initiation factor 1A</fullName>
            <shortName evidence="1">aIF-1A</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">eif1a</name>
        <name type="ordered locus">NP_1258A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-1A family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322356.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IT14"/>
    <dbReference type="SMR" id="Q3IT14"/>
    <dbReference type="STRING" id="348780.NP_1258A"/>
    <dbReference type="EnsemblBacteria" id="CAI48720">
        <property type="protein sequence ID" value="CAI48720"/>
        <property type="gene ID" value="NP_1258A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703261"/>
    <dbReference type="KEGG" id="nph:NP_1258A"/>
    <dbReference type="eggNOG" id="arCOG01179">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109098_1_2_2"/>
    <dbReference type="OrthoDB" id="2586at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05793">
        <property type="entry name" value="S1_IF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00216">
        <property type="entry name" value="aIF_1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006196">
        <property type="entry name" value="RNA-binding_domain_S1_IF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001253">
        <property type="entry name" value="TIF_eIF-1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018104">
        <property type="entry name" value="TIF_eIF-1A_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00523">
        <property type="entry name" value="eIF-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003082">
        <property type="entry name" value="PRK04012.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003083">
        <property type="entry name" value="PRK04012.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003084">
        <property type="entry name" value="PRK04012.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003085">
        <property type="entry name" value="PRK04012.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21668">
        <property type="entry name" value="EIF-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01176">
        <property type="entry name" value="eIF-1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00652">
        <property type="entry name" value="eIF1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01262">
        <property type="entry name" value="IF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50832">
        <property type="entry name" value="S1_IF1_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000259370" description="Translation initiation factor 1A">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="S1-like" evidence="1">
        <location>
            <begin position="6"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00216"/>
        </source>
    </evidence>
    <sequence length="97" mass="11432" checksum="18371827C89E25A0" modified="2005-11-08" version="1">MSDNEGRKNLRMPEGDEVFAVVTNMLGANRVKVRCMDGTERTARIPGRMQKRIWIREDDVVLVEPWDWQDEKADVVWRYEKQEADQLREEGHITDSV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-09-11" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IUG6</accession>
    <name>RL15E_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein eL15</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">50S ribosomal protein L15e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl15e</name>
        <name type="ordered locus">NP_0246A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321853.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IUG6"/>
    <dbReference type="SMR" id="Q3IUG6"/>
    <dbReference type="STRING" id="348780.NP_0246A"/>
    <dbReference type="EnsemblBacteria" id="CAI48214">
        <property type="protein sequence ID" value="CAI48214"/>
        <property type="gene ID" value="NP_0246A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703126"/>
    <dbReference type="KEGG" id="nph:NP_0246A"/>
    <dbReference type="eggNOG" id="arCOG04209">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080796_1_0_2"/>
    <dbReference type="OrthoDB" id="8183at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1120.10:FF:000002">
        <property type="entry name" value="50S ribosomal protein L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1120.10">
        <property type="entry name" value="Ribosomal protein l15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00256">
        <property type="entry name" value="Ribosomal_eL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024794">
        <property type="entry name" value="Rbsml_eL15_core_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000439">
        <property type="entry name" value="Ribosomal_eL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020926">
        <property type="entry name" value="Ribosomal_eL15_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020925">
        <property type="entry name" value="Ribosomal_eL15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003269">
        <property type="entry name" value="PRK04243.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11847:SF4">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11847">
        <property type="entry name" value="RIBOSOMAL PROTEIN L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00827">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01384">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01194">
        <property type="entry name" value="RIBOSOMAL_L15E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000304208" description="Large ribosomal subunit protein eL15">
        <location>
            <begin position="1"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="159"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="179"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="196" mass="22897" checksum="DBAD45EA63D7B2FD" modified="2005-11-08" version="1">MARSFYSHIREAWKTPKEGKLAELQWQRQQEWRDQGAIERIERPTRLDKARSLGYKAKQGVVVARVSVRKGTARKQRFKAGRRSKRQGVNKITRRKNLQRIAEERSGRKFRNLRVLNSYWVGEDGSQKWFEVILLDPEHGAIQNDDDLSWICDDSQRGRAYRGRTSAGQRGRGQQKRGKGTEHTRPSIRANDKRGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETH3</accession>
    <name>A0A1U7ETH3_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Transcription termination factor FttA</fullName>
            <ecNumber evidence="12">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">ncsA</name>
        <name evidence="12" type="synonym">fttA</name>
        <name evidence="17" type="ordered locus">NP_0210A</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Terminates transcription on the whole genome. Termination is linked to FttA-mediated RNA cleavage and does not require NTP hydrolysis. Cleaves endonucleolytically at the RNA exit channel of RNA polymerase (RNAP); the 5'-3' exonuclease activity of this protein degrades the nascent RNA released from RNAP.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12">Binds 2 Zn(2+) ions, which are required for nuclease activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer. Interacts with RNA polymerase (RNAP), interacts with the Spt4-Spt5 complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. FttA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="12"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321835.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETH3"/>
    <dbReference type="STRING" id="348780.NP_0210A"/>
    <dbReference type="EnsemblBacteria" id="CAI48196">
        <property type="protein sequence ID" value="CAI48196"/>
        <property type="gene ID" value="NP_0210A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700789"/>
    <dbReference type="KEGG" id="nph:NP_0210A"/>
    <dbReference type="eggNOG" id="arCOG00543">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009673_5_1_2"/>
    <dbReference type="OrthoDB" id="7155at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004532">
        <property type="term" value="F:RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22532">
        <property type="entry name" value="KH-II_CPSF_arch_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02410">
        <property type="entry name" value="KH-II_CPSF_arch_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16295">
        <property type="entry name" value="TTHA0252-CPSF-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00870">
        <property type="entry name" value="FttA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019975">
        <property type="entry name" value="aCPSF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050698">
        <property type="entry name" value="MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033769">
        <property type="entry name" value="TffA_KH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03675">
        <property type="entry name" value="arCOG00543"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203:SF51">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10996">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17214">
        <property type="entry name" value="KH_TffA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01027">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="Prokaryotic type KH domain (KH-domain type II)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 12" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 12" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 12" id="KW-0540">Nuclease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12 13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 12" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="6 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="K Homology" evidence="14">
        <location>
            <begin position="98"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="15">
        <location>
            <begin position="194"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-Casp" evidence="16">
        <location>
            <begin position="423"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="KHa" evidence="12">
        <location>
            <begin position="5"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="KHb" evidence="12">
        <location>
            <begin position="73"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Metallo-beta-lactamase N-terminus" evidence="12">
        <location>
            <begin position="181"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Metallo-beta-lactamase C-terminus" evidence="12">
        <location>
            <begin position="581"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="354"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="354"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="606"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAI48196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="639" mass="71326" checksum="37F67D93E7F57A4B" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETJ1</accession>
    <name>A0A1U7ETJ1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable sugar dehydrogenase (Homolog to aldose sugar dehydrogenase)</fullName>
            <ecNumber evidence="2">1.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0254A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321857.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETJ1"/>
    <dbReference type="STRING" id="348780.NP_0254A"/>
    <dbReference type="EnsemblBacteria" id="CAI48218">
        <property type="protein sequence ID" value="CAI48218"/>
        <property type="gene ID" value="NP_0254A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700804"/>
    <dbReference type="KEGG" id="nph:NP_0254A"/>
    <dbReference type="eggNOG" id="arCOG02796">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012253_0_1_2"/>
    <dbReference type="OrthoDB" id="6744at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012938">
        <property type="entry name" value="Glc/Sorbosone_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011041">
        <property type="entry name" value="Quinoprot_gluc/sorb_DH_b-prop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19328:SF75">
        <property type="entry name" value="ALDOSE SUGAR DEHYDROGENASE YLII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19328">
        <property type="entry name" value="HEDGEHOG-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07995">
        <property type="entry name" value="GSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50952">
        <property type="entry name" value="Soluble quinoprotein glucose dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose/Sorbosone dehydrogenase" evidence="1">
        <location>
            <begin position="53"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="397" mass="43284" checksum="C85CF9978CFEC01E" modified="2017-05-10" version="1">MPRFDRRDYLRAAGLAGAAALAGCTDVFDDNGETPDAPEETEFTVETVADGFAHPWGMAFLPNDEQLLVTEREGSLSLLDRESGDVDRLDGLPDVHAAGQGGLLDIALDPDFESEPWVYLTYAATNGQGESTTCLGRGRLAAADGRLEDFERLYTAEPFVDSTQHYGSRVVFGEDGMVYMTTGDRGDKDFGPDHVSQDPSNDLGATLRLAPDGTVPEDNPFTDDPDINDAIFSYGHRNAQGMDIHPDTGDIWQSEHGERDGDEINIIERGGNHGWPVVHYGCEYGTDEPVGEQPHERDDTVGPVYYWECGSGGFPPSGAMFYDGDAFPEWRGDLFVGNLAGQYLGRFTVDGTVVEETSALLADRDWRIRAIETAPDTDFIYVAVDAGDAPIVRLRPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETJ6</accession>
    <name>A0A1U7ETJ6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0290A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321875.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETJ6"/>
    <dbReference type="STRING" id="348780.NP_0290A"/>
    <dbReference type="EnsemblBacteria" id="CAI48236">
        <property type="protein sequence ID" value="CAI48236"/>
        <property type="gene ID" value="NP_0290A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700811"/>
    <dbReference type="KEGG" id="nph:NP_0290A"/>
    <dbReference type="eggNOG" id="arCOG04700">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_168012_0_0_2"/>
    <dbReference type="OrthoDB" id="156156at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045940">
        <property type="entry name" value="DUF6360"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19887">
        <property type="entry name" value="DUF6360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="93" mass="10360" checksum="8A29B11B27711175" modified="2017-05-10" version="1">MVDRIMKVNAYTTLDLLDGEAEGHGFEEDAYAVLNVTSPKKNPDHVSLQLELDNTELETLRPHADKVRLSASEARTLAADLEKHAEKVENESD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETK5</accession>
    <name>A0A1U7ETK5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">homoserine dehydrogenase</fullName>
            <ecNumber evidence="2">1.1.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">hom</name>
        <name evidence="8" type="ordered locus">NP_0302A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the homoserine dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.3" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321880.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETK5"/>
    <dbReference type="STRING" id="348780.NP_0302A"/>
    <dbReference type="EnsemblBacteria" id="CAI48242">
        <property type="protein sequence ID" value="CAI48242"/>
        <property type="gene ID" value="NP_0302A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702827"/>
    <dbReference type="KEGG" id="nph:NP_0302A"/>
    <dbReference type="eggNOG" id="arCOG01351">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009116_1_2_2"/>
    <dbReference type="OrthoDB" id="4488at2157"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00063"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00465"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004412">
        <property type="term" value="F:homoserine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000005">
        <property type="entry name" value="Homoserine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001342">
        <property type="entry name" value="HDH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019811">
        <property type="entry name" value="HDH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022697">
        <property type="entry name" value="HDH_short"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004976">
        <property type="entry name" value="PRK06349.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43331">
        <property type="entry name" value="HOMOSERINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43331:SF1">
        <property type="entry name" value="HOMOSERINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00742">
        <property type="entry name" value="Homoserine_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036497">
        <property type="entry name" value="HDH_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01042">
        <property type="entry name" value="HOMOSER_DHGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="3 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal" evidence="6">
        <location>
            <begin position="3"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="Homoserine dehydrogenase catalytic" evidence="7">
        <location>
            <begin position="148"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="7"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>L-homoserine</name>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR036497-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR036497-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI48242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="315" mass="32163" checksum="21E8A1AF152D5943" modified="2017-05-10" version="1">MKLSVLGSGAVGAAVVELAGEYGHEVVAVADSASAAVDESGLDAEAVLERKRTDGRVGDADADAALEADYDVLVEATPTTLGDAEPGFSHVKQALERDRHVVLANKGPVAERYDELRSLEADSAGSVRFEAAVGGAIPILSTIADLDGQVTAARGVLNGTANFILSRMAAEGLGYEHVLAEAQDLGVAEADPTFDVEGTDAALKCVILANVLYEDTYTLEDASVEGIADLPGSALELAAEDGRTIRLIGDVSDGEVSVAPRLVPENGTLAVSGTLNIIQLETAHAGQLNISGRGAGGPETASAVLADVGRLPPLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETM6</accession>
    <name>A0A1U7ETM6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription factor S</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Transcription elongation factor IIS/RNA polymerase subunit homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">tfs1</name>
        <name evidence="15" type="synonym">rpoM1</name>
        <name evidence="15" type="ordered locus">NP_0364A</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="8 12">Belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321911.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETM6"/>
    <dbReference type="STRING" id="348780.NP_0364A"/>
    <dbReference type="EnsemblBacteria" id="CAI48273">
        <property type="protein sequence ID" value="CAI48273"/>
        <property type="gene ID" value="NP_0364A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703161"/>
    <dbReference type="KEGG" id="nph:NP_0364A"/>
    <dbReference type="eggNOG" id="arCOG00579">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093932_3_2_2"/>
    <dbReference type="OrthoDB" id="72957at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10511">
        <property type="entry name" value="Zn-ribbon_TFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019761">
        <property type="entry name" value="DNA-dir_RNA_pol-M_15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012164">
        <property type="entry name" value="Rpa12/Rpb9/Rpc10/TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006288">
        <property type="entry name" value="TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001529">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01384">
        <property type="entry name" value="TFS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239:SF12">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02150">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005586">
        <property type="entry name" value="RNApol_RpoM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00661">
        <property type="entry name" value="RPOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01030">
        <property type="entry name" value="RNA_POL_M_15KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="2 9 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS-type" evidence="14">
        <location>
            <begin position="64"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="10">
        <location>
            <begin position="4"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="27"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="37"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="4"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
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    <feature type="binding site" evidence="9">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR005586-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAI48273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="108" mass="12362" checksum="9A41DC4CF433F96B" modified="2017-05-10" version="1">MEFCDECGSMMHSEDGLWVCRSCGFEKQQGDDDADRMTTTEGQDTDSGPVDMSEVDDSEIGPTTRVNCPECGNDRARYEMKQIRAADESETRFFTCTECEHKWREDDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETM9</accession>
    <name>A0A1U7ETM9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Major facilitator superfamily transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tp12</name>
        <name evidence="7" type="ordered locus">NP_0366A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="CAI48274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321912.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETM9"/>
    <dbReference type="STRING" id="348780.NP_0366A"/>
    <dbReference type="EnsemblBacteria" id="CAI48274">
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        <property type="gene ID" value="NP_0366A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703288"/>
    <dbReference type="KEGG" id="nph:NP_0366A"/>
    <dbReference type="eggNOG" id="arCOG00130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_10_14_2"/>
    <dbReference type="OrthoDB" id="117970at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="MFS_MdtG_SLC18_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
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        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23518">
        <property type="entry name" value="C-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="46"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="114"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="238"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="270"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="303"/>
            <end position="322"/>
        </location>
    </feature>
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            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="378"/>
        </location>
    </feature>
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        <location>
            <begin position="384"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="1"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="426" mass="44188" checksum="1EC4B44933AEE202" modified="2017-05-10" version="1">MFGTDRRVVVLALARMADAVANSFLIIVLPLYIASGELALPAFEGASVLGIPLTVELLIGVALSLFGFVNSFAQPFTGRLSDRTARRKIYILFGLVVLAVTSVAYALVESYEALLVVRALQGLGGAFVIPTTIALVNDLARSDAERGGNFGVFNTFRLIGFGFGPIVAGVVVQAGPYALPLGTELSGFNAAFGVAVLGALVSFTLVTVLIEEPPMAAADATDDLSIAVFGDSRLLDPVFVLGVATFFMAVSIALFATLQEPINDRLSQGPLWFGIQFAAVVVANVILQMPIGRAADRIGRRPFLVWGLVLLVPAVLAQGLVTSSWTMLVVRLLHGVSVAMVFAPALAVAGDLAEEGESGTTLSVLTMAFGLGTAVGPLSSGVLFGYAFVVPFAAGAVLAFLALVLVATQVEETLETATGGPILGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETP1</accession>
    <name>A0A1U7ETP1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Diphthamide biosynthesis protein Dph6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">dph6</name>
        <name evidence="2" type="ordered locus">NP_0382A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321920.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETP1"/>
    <dbReference type="STRING" id="348780.NP_0382A"/>
    <dbReference type="EnsemblBacteria" id="CAI48282">
        <property type="protein sequence ID" value="CAI48282"/>
        <property type="gene ID" value="NP_0382A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700837"/>
    <dbReference type="KEGG" id="nph:NP_0382A"/>
    <dbReference type="eggNOG" id="arCOG00035">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010289_0_2_2"/>
    <dbReference type="OrthoDB" id="372052at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017178">
        <property type="term" value="F:diphthine-ammonia ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01994">
        <property type="entry name" value="AANH_PF0828-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1490.10">
        <property type="entry name" value="putative n-type atp pyrophosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002761">
        <property type="entry name" value="Diphthami_syn_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030662">
        <property type="entry name" value="DPH6/MJ0570"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022427">
        <property type="entry name" value="MJ0570_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03679">
        <property type="entry name" value="arCOG00187"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00290">
        <property type="entry name" value="MJ0570_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12196:SF2">
        <property type="entry name" value="DIPHTHINE--AMMONIA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12196">
        <property type="entry name" value="DOMAIN OF UNKNOWN FUNCTION 71 DUF71 -CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01902">
        <property type="entry name" value="Diphthami_syn_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039123">
        <property type="entry name" value="Diphthamide_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Diphthamide synthase" evidence="1">
        <location>
            <begin position="5"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="239" mass="26046" checksum="BD601CDC00067F8F" modified="2017-05-10" version="1">MKPWVSLFSGGKDSSWALYRALEDGKPVERLVTVHPEGDSFMYHVPATELASLAAESIGIPLVNVRPDDFDDGATAAEDSSQRGDRELEPLEAELSALADELGGIDGVTAGAVESSYQTDRLEGLCDRLDAELFAPLWQQNPRELAEAMFAAGFEITIVSVSARGLDESWLGRTLDAEALAELERLNDEYGVHVLGEGGEFETLVTDAPHMDRRIELEYETTFDGLRGSLQITDAWLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETR0</accession>
    <name>A0A1U7ETR0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Alanine dehydrogenase</fullName>
            <shortName evidence="1">AlaDH</shortName>
            <ecNumber evidence="1">1.4.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1 2" type="primary">ala</name>
        <name evidence="2" type="synonym">ocd1:arcB1</name>
        <name evidence="2" type="ordered locus">NP_0448A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
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            <dbReference type="DOI" id="10.1101/gr.3952905"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NAD(+)-dependent oxidative deamination of L-alanine to pyruvate, and the reverse reaction, the reductive amination of pyruvate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-alanine + NAD(+) + H2O = pyruvate + NH4(+) + NADH + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:15361"/>
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            <dbReference type="ChEBI" id="CHEBI:57972"/>
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        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ornithine cyclodeaminase/mu-crystallin family. Archaeal alanine dehydrogenase subfamily.</text>
    </comment>
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        <property type="gene ID" value="NP_0448A"/>
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    <dbReference type="eggNOG" id="arCOG01035">
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    </dbReference>
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        <property type="entry name" value="KETIMINE REDUCTASE MU-CRYSTALLIN"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0520">NAD</keyword>
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    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="71"/>
        </location>
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        <location>
            <position position="115"/>
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        <ligand>
            <name>NAD(+)</name>
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        </ligand>
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        <ligand>
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        <ligand>
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        </ligand>
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        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
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        </ligand>
    </feature>
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        <location>
            <position position="296"/>
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        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="331" mass="35582" checksum="E1DBDED98A867BE1" modified="2017-05-10" version="1">METLLLDPDDVDAAADMAEVIVAVEEAFAAYERGDVQMPAKSYIDLPEYNGDFRSMPAYMEADDWDAAGIKWVNVHTDNPDEYDLPTVMGTMVYSDPRNAFPLSIMDGTTLTRKRTGAAAAVATDHLAIEDANSLGIVGAGVQSYTQLEAISVVRDIDEVVVADKRDEAVEAFIDHFGDEFDVRRGSIAEAASCDVLSTVTPVRSPIVSADDLADHTHINAMGADAPDKNELEQDVVTDARIVIDDHEQCTHSGEINVPWSEGVLDDADISAELGEIVVGDEPRRRDSDGVTVFDSTGLAIQDVAAARAVYERATADGAGTSFGLVDTKLR</sequence>
</entry>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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                <person name="Pfeiffer F."/>
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                <person name="Rodewald K."/>
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            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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        <property type="gene ID" value="NP_0540A"/>
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    <dbReference type="KEGG" id="nph:NP_0540A"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        </location>
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        </location>
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        </location>
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        </location>
    </feature>
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        <location>
            <begin position="8"/>
            <end position="27"/>
        </location>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF06808"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI48361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="911" mass="96512" checksum="ACFB3527B30D1332" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETW3</accession>
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        <submittedName>
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        <name evidence="7" type="primary">thiDN</name>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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            <authorList>
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                <person name="Pfeiffer F."/>
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                <person name="Rodewald K."/>
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            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
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        <property type="gene ID" value="NP_0546A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703245"/>
    <dbReference type="KEGG" id="nph:NP_0546A"/>
    <dbReference type="eggNOG" id="arCOG00020">
        <property type="taxonomic scope" value="Archaea"/>
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        <property type="term" value="C:cytosol"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008972">
        <property type="term" value="F:phosphomethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004789">
        <property type="term" value="F:thiamine-phosphate diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01169">
        <property type="entry name" value="HMPP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.20:FF:000003">
        <property type="entry name" value="Phosphomethylpyrimidine kinase ThiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004399">
        <property type="entry name" value="HMP/HMP-P_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019293">
        <property type="entry name" value="ThiN"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00097">
        <property type="entry name" value="HMP-P_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858:SF17">
        <property type="entry name" value="HYDROXYMETHYLPYRIMIDINE_PHOSPHOMETHYLPYRIMIDINE KINASE THI20-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858">
        <property type="entry name" value="PHOSPHOMETHYLPYRIMIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10120">
        <property type="entry name" value="ThiN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyridoxamine kinase/Phosphomethylpyrimidine kinase" evidence="5">
        <location>
            <begin position="22"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine-phosphate synthase ThiN" evidence="6">
        <location>
            <begin position="281"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="452" mass="46619" checksum="5A49965E0B4DAA6B" modified="2017-05-10" version="1">MDGTRTPAPTEKPVVLTIAGSDSGGGAGIQADLKTIEATGGFGTSAVTAVTAQNTTGVASSHVLPVEELAAQIEAVTDDFDVRAVKTGMLATTEVVETVTQRVADLSCPVVVDPVMVAATGDRLLTEAAESAYEDLLAAATLATPNADEAAVLTGSEPEDEAEARAAGQEIIALGADAALVKGGHMPGDAVTDVLVADSGTEVFSHPRIDTDATHGSGCTLSAAIATHLASGAPLSTAVERGVDFMERAVRYHHSVGSGPGAVHHLVDLRDDAVRDTVAESVQDAVDRLVAEDVSPAIPQVGMNVVAATPYAETTADIAAVEGRLTRAVSGVARGRGVRFGASSHVARFLLAAREHRPDLAAAANCRFDATVESALERLDWTVAEYDRDAEPESAESTMQWAATEVFADGADPVAIIDRGDVGKEPMMKIVATSPETVVSQLIEAADELRER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETY0</accession>
    <name>A0A1U7ETY0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ORC1-type DNA replication protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">orc1</name>
        <name evidence="9" type="synonym">cdc6A</name>
        <name evidence="9" type="ordered locus">NP_0596A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in regulation of DNA replication.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the CDC6/cdc18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48389.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322025.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETY0"/>
    <dbReference type="STRING" id="348780.NP_0596A"/>
    <dbReference type="EnsemblBacteria" id="CAI48389">
        <property type="protein sequence ID" value="CAI48389"/>
        <property type="gene ID" value="NP_0596A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702590"/>
    <dbReference type="KEGG" id="nph:NP_0596A"/>
    <dbReference type="eggNOG" id="arCOG00467">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025112_3_1_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08768">
        <property type="entry name" value="Cdc6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18139">
        <property type="entry name" value="HLD_clamp_RarA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000073">
        <property type="entry name" value="ORC1-type DNA replication protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01407">
        <property type="entry name" value="ORC1_type_DNA_replic_protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015163">
        <property type="entry name" value="Cdc6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055237">
        <property type="entry name" value="Cdc6_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050311">
        <property type="entry name" value="ORC1/CDC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014277">
        <property type="entry name" value="Orc1/Cdc6_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02928">
        <property type="entry name" value="orc1/cdc6 family replication initiation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763:SF22">
        <property type="entry name" value="ORC1-TYPE DNA REPLICATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22703">
        <property type="entry name" value="Cdc6_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09079">
        <property type="entry name" value="WHD_Cdc6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01074">
        <property type="entry name" value="Cdc6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="163"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Cdc6 C-terminal" evidence="8">
        <location>
            <begin position="467"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="175"/>
            <end position="179"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="372"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="384"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI48389.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="571" mass="63674" checksum="954416C531C3D94C" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU11</accession>
    <name>A0A1U7EU11_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Homolog to S-adenosylmethionine-dependent methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0672A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322063.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU11"/>
    <dbReference type="STRING" id="348780.NP_0672A"/>
    <dbReference type="EnsemblBacteria" id="CAI48427">
        <property type="protein sequence ID" value="CAI48427"/>
        <property type="gene ID" value="NP_0672A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700924"/>
    <dbReference type="KEGG" id="nph:NP_0672A"/>
    <dbReference type="eggNOG" id="arCOG06202">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056435_5_3_2"/>
    <dbReference type="OrthoDB" id="147504at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="2">
        <location>
            <begin position="36"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="211" mass="23154" checksum="DA584D3512E643DB" modified="2017-05-10" version="1">MVDWDERFESGSYPQHPDPAPVLKRYVDGSGDGRALDVATGTGRNAVFLAEHGYAVDALDKSRAGLETTRRNAAERGVAGNLNCIQTDIPSHTFPEGTYDIVTVSFYRAVDRFPDLKAALAPGGLFFVEHHLRTADPVEGGPSGDRYRFAANELLNSCLDLTVLSYEEGIEVRDDGRESAIARIVARNASGRQSYPRIREWQRQESGGGRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU50</accession>
    <name>A0A1U7EU50_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC-type transport system permease protein (Probable substrate dipeptide/oligopeptide)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">dppB1</name>
        <name evidence="9" type="synonym">abc01p1</name>
        <name evidence="9" type="ordered locus">NP_0760A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322107.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU50"/>
    <dbReference type="STRING" id="348780.NP_0760A"/>
    <dbReference type="EnsemblBacteria" id="CAI48471">
        <property type="protein sequence ID" value="CAI48471"/>
        <property type="gene ID" value="NP_0760A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702364"/>
    <dbReference type="KEGG" id="nph:NP_0760A"/>
    <dbReference type="eggNOG" id="arCOG00751">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036879_0_0_2"/>
    <dbReference type="OrthoDB" id="44105at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045621">
        <property type="entry name" value="BPD_transp_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43163">
        <property type="entry name" value="DIPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN DPPB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43163:SF6">
        <property type="entry name" value="DIPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN DPPB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19300">
        <property type="entry name" value="BPD_transp_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="135"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="251"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="301"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="97"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI48471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="329" mass="35460" checksum="4887A822D55AA994" modified="2017-05-10" version="1">MSLAKFLAKRTLQGLFVIWGVVTVVFFLRFLTPGDPTIALTPPDVSDERRAELAAELGLNDPLHVQYTDYLFQLARGDLGEAMASGRNANTVLLERLPATIELAVAATVVAVIIAIPLGVISARRRHEPADYGATLFSLVGISTPNFWLGIMLIIFVAVSVDFLPTGGRHITFTAAWGGLLFEGDISGVGTWFAQLILPAIALGTYFTALITRLTRSGMLEELGKAYVKACRAKGLPETLTMYKHVLRNTLIPILTVLGLQLGTLIGGAIITEEVFAWPGLGTLLIDAIGERDWTLIQGSIIIIGIGFVVINIAVDALYAYLDPRVSAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU81</accession>
    <name>A0A1U7EU81_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Mrp-type sodium/proton antiporter system subunit C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">mrpC</name>
        <name evidence="6" type="synonym">tp49f</name>
        <name evidence="6" type="ordered locus">NP_0832A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322143.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU81"/>
    <dbReference type="STRING" id="348780.NP_0832A"/>
    <dbReference type="EnsemblBacteria" id="CAI48507">
        <property type="protein sequence ID" value="CAI48507"/>
        <property type="gene ID" value="NP_0832A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703332"/>
    <dbReference type="KEGG" id="nph:NP_0832A"/>
    <dbReference type="eggNOG" id="arCOG03072">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082058_2_0_2"/>
    <dbReference type="OrthoDB" id="18006at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050601">
        <property type="entry name" value="CPA3_antiporter_subunitC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039428">
        <property type="entry name" value="NUOK/Mnh_C1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34583">
        <property type="entry name" value="ANTIPORTER SUBUNIT MNHC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34583:SF3">
        <property type="entry name" value="MULTISUBUNIT SODIUM_HYDROGEN ANTIPORTER, MNHC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00420">
        <property type="entry name" value="Oxidored_q2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="36"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="73"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI48507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="127" mass="13792" checksum="24266EE26534935D" modified="2017-05-10" version="1">MIDGTYHYYLTAFVLFLIGIYMMIDNRNLIKKVIGLNFAQISVYLMLVTIGYVEDSNPPIIGFGEPHSNPLVHVLVLTAIVVGVSLTALALALVIRLYSEFGTLDAREIEAKIAASEGGDKGGDSDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUE1</accession>
    <name>A0A1U7EUE1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">IS1341-type transposase ISNph23</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">NP_0974A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322213.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUE1"/>
    <dbReference type="STRING" id="348780.NP_0974A"/>
    <dbReference type="EnsemblBacteria" id="CAI48578">
        <property type="protein sequence ID" value="CAI48578"/>
        <property type="gene ID" value="NP_0974A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701016"/>
    <dbReference type="KEGG" id="nph:NP_0974A"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_3_3_2"/>
    <dbReference type="OrthoDB" id="210698at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF26">
        <property type="entry name" value="TRANSPOSASE, PROBABLY IS605-TNPB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="6">
        <location>
            <begin position="186"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="7">
        <location>
            <begin position="304"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI48578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="414" mass="47461" checksum="B7544079F6DE9345" modified="2017-05-10" version="1">MEVRRTLPVKLLVADSNRDALLETIDQYKDCANETSDHCWDENDYKKTAKYAVKDELYHDLKAEHDLTANLVQQAIFQAVEAVKAGVERLEKNEDTSQPEFTADTARYDKRAATFHDDHVSLSTVDGRIEADYVLPVDETNTPFEEYLNGDEWEFRTSTLHYKPFDDEFWLHIGFKRNKEATDSTQPDAAVPEHNTVLGIDLGVENLAVASTGTFWTGDELDHWHREFQQRRGNLQQVGSQEAHRTLQRVSKKETAYYKQYLHVVANEIVEEAVEHDCSVIAFEDLTDIRKRAQGATWHHRWRFNRLYEYVEYKAKAYGIDVEELERSPQAKTVPAYYTSQRCSSCGLTHEDNRPTQAHFECQKCGYENHADYNAAKNVAVRCCRRLLRRNQTGGDGGAPVDVALNGGTMTVNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUE9</accession>
    <name>A0A1U7EUE9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Tetratricopeptide repeat protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0994A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322223.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUE9"/>
    <dbReference type="STRING" id="348780.NP_0994A"/>
    <dbReference type="EnsemblBacteria" id="CAI48588">
        <property type="protein sequence ID" value="CAI48588"/>
        <property type="gene ID" value="NP_0994A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701024"/>
    <dbReference type="KEGG" id="nph:NP_0994A"/>
    <dbReference type="eggNOG" id="arCOG03032">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_942013_0_0_2"/>
    <dbReference type="OrthoDB" id="115601at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558">
        <property type="entry name" value="CELL DIVISION CYCLE 16,23,27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558:SF13">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 27 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="62"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="99"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="218"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="232"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="245" mass="27670" checksum="6C92200C6D2337ED" modified="2017-05-10" version="1">MTDDEPSDHAFSAGQGFEDPYEGFDIDPPELDVDPGMVDPVDSRVVADMLDRRQISDNAVDAESLLDVGLEYMRINRFEQATDALERAASFADDETIEQEAWVNKGAAHAELEEHQRAIDAYREALRIDDESEHAASAETNLAYALWNDGRSEQALEHAERAVELDQRFAQGWYNRGFFLLERGLAEDAVSCFDNAIRLGYRNADVLQEKARALEEIGEDEKATELAEEAEELRQEAERELVDQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUI6</accession>
    <name>A0A1U7EUI6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">UCP012666 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1074A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322264.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUI6"/>
    <dbReference type="STRING" id="348780.NP_1074A"/>
    <dbReference type="EnsemblBacteria" id="CAI48628">
        <property type="protein sequence ID" value="CAI48628"/>
        <property type="gene ID" value="NP_1074A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701048"/>
    <dbReference type="KEGG" id="nph:NP_1074A"/>
    <dbReference type="eggNOG" id="arCOG04732">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029030_1_0_2"/>
    <dbReference type="OrthoDB" id="194541at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050141">
        <property type="entry name" value="GCL_type2/YbdK_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006336">
        <property type="entry name" value="GCS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016602">
        <property type="entry name" value="UCP012666"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36510:SF3">
        <property type="entry name" value="CONSERVED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36510">
        <property type="entry name" value="GLUTAMATE--CYSTEINE LIGASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04107">
        <property type="entry name" value="GCS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF012666">
        <property type="entry name" value="UCP012666"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="501" mass="56372" checksum="47035E8867FDF773" modified="2017-05-10" version="1">MSASELAAQIEAALSVDREEFASRVEAEKEVLRGEIEDGTFDNPQAIVGLEYEFYAVDAETDALMRVPRRLLEYIGFEKELGLHNAELSTTPQPLSEYGLRAQEAEVQAKLDAAETEVNREGMALVSDGLWTVPPVGETAAAYLTDSIEEDGMRMAANMSRAVRYHAMANTEYAPELQLDAPHVSLSSETVMPESLITSIQPHYQVPHAHDLPTYFRYALRVAGPLLAVAVNSPFFPPDLYDADGETVLDDCWMENRVPVFESVLNPADERKVSFPKDIDSVDEALDRIAADETVVPADVDPEGRFDDEFAHFRHKHGSFWRWVRPVFGGPTRTSANARIEFRPLPGQPTVRDTISLLAVFAGLMESLPRREHPIYHQDWATAEENFYRAVRDGLAADIRWRTADGTETTDTEELYAELFEIARDGLELRGLDAEASNRYIQPLRERVDRRVTPARWKYEHVADAVADGEPFAQAVWGMQAEYIENQSETLIEGAFVDWLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUL1</accession>
    <name>A0A1U7EUL1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1172A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322313.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUL1"/>
    <dbReference type="STRING" id="348780.NP_1172A"/>
    <dbReference type="EnsemblBacteria" id="CAI48677">
        <property type="protein sequence ID" value="CAI48677"/>
        <property type="gene ID" value="NP_1172A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701074"/>
    <dbReference type="KEGG" id="nph:NP_1172A"/>
    <dbReference type="eggNOG" id="arCOG02980">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027187_0_0_2"/>
    <dbReference type="OrthoDB" id="85977at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047792">
        <property type="entry name" value="Hvo_1808-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038145">
        <property type="entry name" value="Hvo_1808_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="468" mass="51015" checksum="B9354A2CFFC32339" modified="2017-05-10" version="1">MDRRLLAATALAAVLLSAGCTVGYQPAADAPPTSEPPAEPHPGYHDGYWYNDTFDLDAEAGLTDPQVDAVTARAMARVQLLRGLEFESDVDVELLTREAFQAEYGDVWGDPPADAQTLDNVQHEALFLVESDEDVTDARAVNRGDTVLGFYQPTTERLVVVSENDPATFDDEMTLAHELLHALQDQHFGLEDVGDGTIDGTNARNGLIEGDAVVIETEYEARCETDEWQCLGTDDDGGTVGVPAAFNWGLYVLDFFPYTAGPPFIDHHRERGGWSAVDAAYDAPPTASAEVIYPETYGSGTYGEARIEDQPRNGWQRVTVDNGTDHARVGQAGLVAMFARTAYDDRASGVVPHTAFTGDDTRYTYDIEYADGWTGDRLHAYQRDNETAYRWNVTFENADEAATFRRGYERVLDYWGGEETADGHWRFDGAAGFEKTVSVSDADASVRVVGAPTPEAVGDISPAVAPNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUV8</accession>
    <name>A0A1U7EUV8_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable queuosine precursor transporter</fullName>
            <shortName evidence="1">Q precursor transporter</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1354A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the import of queuosine (Q) precursors, required for Q precursor salvage.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the vitamin uptake transporter (VUT/ECF) (TC 2.A.88) family. Q precursor transporter subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUV8"/>
    <dbReference type="STRING" id="348780.NP_1354A"/>
    <dbReference type="EnsemblBacteria" id="CAI48768">
        <property type="protein sequence ID" value="CAI48768"/>
        <property type="gene ID" value="NP_1354A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_1354A"/>
    <dbReference type="eggNOG" id="arCOG04284">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075503_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02088">
        <property type="entry name" value="Q_prec_transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003744">
        <property type="entry name" value="YhhQ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00697">
        <property type="entry name" value="queuosine precursor transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34300">
        <property type="entry name" value="QUEUOSINE PRECURSOR TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34300:SF2">
        <property type="entry name" value="QUEUOSINE PRECURSOR TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02592">
        <property type="entry name" value="Vut_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="242" mass="25636" checksum="03BD9BF7A60EE7CC" modified="2017-05-10" version="1">MRTMSDTARRPSPPAGAVFLAAVFVASLATAQLTAAKVLSFDLPFALPLAGAELALPGAALAYALTFLASDCYTELYGKRAAQVLVNVAFAMNFLVLALVWSTILAPAAPSSIDPESFELVLGASTNIVAGSLLAYVVSQNYDVVVFHRLGELTDGDHLWLRNIASTGTSQLLDTVIFVSIAFWVFPQYVGLGPELPAAAVVSLMVGQYLLKLLIAVVDTPFVYLITGAVRRYTDPAPSPAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV01</accession>
    <name>A0A1U7EV01_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Probable glycosyltransferase, type 1</fullName>
            <ecNumber evidence="3">2.4.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">gth3</name>
        <name evidence="3" type="ordered locus">NP_1476A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.4.-.-" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322464.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV01"/>
    <dbReference type="STRING" id="348780.NP_1476A"/>
    <dbReference type="EnsemblBacteria" id="CAI48829">
        <property type="protein sequence ID" value="CAI48829"/>
        <property type="gene ID" value="NP_1476A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701158"/>
    <dbReference type="KEGG" id="nph:NP_1476A"/>
    <dbReference type="eggNOG" id="arCOG01403">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_793672_0_0_2"/>
    <dbReference type="OrthoDB" id="270666at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03801">
        <property type="entry name" value="GT4_PimA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12526">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12526:SF630">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13579">
        <property type="entry name" value="Glyco_trans_4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase subfamily 4-like N-terminal" evidence="2">
        <location>
            <begin position="19"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="1">
        <location>
            <begin position="174"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="351" mass="39057" checksum="AA07ED252F172E62" modified="2017-05-10" version="1">MQVAVVFRDPPPQSERTGAVRLRRLAAAIQSAGHEVTVYCQPWWETDQRRIEINGLVHEGVAFEHPALFYTRLPGVLARRGPDVVLTSASPPGGVVAAWLGGLFARAPVVCDWYGDEPNAMSSRWAGKAASLPTRVVAPSELQRTRIRELGGTEANTTTIPLGISMSKIQAADPDEFRDIVYAGHLDDDANLESLLLALAELRDDGDWTATVIGDGPRRDDYERQARDLRILDRIDFRGNCDRAERIAVYRGAHTFVQTARRANFAEELLWALACGCVGVVEMQSDSSAHELVERRERGFRVTDMENLDEAIEAAWDCSYRDIDKAFQQFDHAAVAGKYLELFRDCGVESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV02</accession>
    <name>A0A1U7EV02_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Enoyl-CoA hydratase</fullName>
            <ecNumber evidence="4">4.2.1.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">fadA5</name>
        <name evidence="4" type="ordered locus">NP_1462A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.17" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322457.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV02"/>
    <dbReference type="STRING" id="348780.NP_1462A"/>
    <dbReference type="DNASU" id="3702682"/>
    <dbReference type="EnsemblBacteria" id="CAI48822">
        <property type="protein sequence ID" value="CAI48822"/>
        <property type="gene ID" value="NP_1462A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702682"/>
    <dbReference type="KEGG" id="nph:NP_1462A"/>
    <dbReference type="eggNOG" id="arCOG00241">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_2_2"/>
    <dbReference type="OrthoDB" id="27846at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.12.10:FF:000001">
        <property type="entry name" value="Probable enoyl-CoA hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43459:SF1">
        <property type="entry name" value="EG:BACN32G11.4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43459">
        <property type="entry name" value="ENOYL-COA HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="263" mass="28050" checksum="F807D07BD5F14E5B" modified="2017-05-10" version="1">MSDPVLVDIEDGIATLTLNRPDNRNALSDGMSRAIVDAVDDIEASDDVRCLVVTGDEGTFCAGGDVNSMVELMSGQRELHEAVDQIQRVTSRAIQRIAEFPLPTIAKVDGVAYGAGANLAIAADITLASERAKISFGFRQVGLAIDTGTSYFLPRQVGLSKAKELVFTGEMLDAEAAEELGLFNHVFEDDFEAEVEAFIEPIASGPTVALQTSKQLIEQGFGSSLKDALDNEASAQAAVFATDDHEEGATAFMEGREPEFEGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV08</accession>
    <name>A0A1U7EV08_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">SirR/DtxR family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">NP_1496A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DtxR/MntR family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322473.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV08"/>
    <dbReference type="STRING" id="348780.NP_1496A"/>
    <dbReference type="EnsemblBacteria" id="CAI48839">
        <property type="protein sequence ID" value="CAI48839"/>
        <property type="gene ID" value="NP_1496A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701161"/>
    <dbReference type="KEGG" id="nph:NP_1496A"/>
    <dbReference type="eggNOG" id="arCOG02100">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069532_4_0_2"/>
    <dbReference type="OrthoDB" id="311420at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050536">
        <property type="entry name" value="DtxR_MntR_Metal-Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001367">
        <property type="entry name" value="Fe_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022687">
        <property type="entry name" value="HTH_DTXR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022689">
        <property type="entry name" value="Iron_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33238">
        <property type="entry name" value="IRON (METAL) DEPENDENT REPRESSOR, DTXR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33238:SF7">
        <property type="entry name" value="IRON-DEPENDENT TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02742">
        <property type="entry name" value="Fe_dep_repr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01325">
        <property type="entry name" value="Fe_dep_repress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00529">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50944">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH dtxR-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI48839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="145" mass="16287" checksum="06817BE32BEE186E" modified="2017-05-10" version="1">MNTQAQYLKAIYLVQEREDGPASTGAIADMLEVSPASANEMIGKLEDQGLADHEKYKGVSLTDEGIVQAREALETYCIIERFLWEVLEVEDFRTEARQLEAVIDETVAERLDTIIDRNDRCPDCFDAEADACELLDIELETEPAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV25</accession>
    <name>A0A1U7EV25_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">HTH-10 family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_1538A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322494.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV25"/>
    <dbReference type="STRING" id="348780.NP_1538A"/>
    <dbReference type="EnsemblBacteria" id="CAI48860">
        <property type="protein sequence ID" value="CAI48860"/>
        <property type="gene ID" value="NP_1538A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701174"/>
    <dbReference type="KEGG" id="nph:NP_1538A"/>
    <dbReference type="eggNOG" id="arCOG02280">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090893_0_0_2"/>
    <dbReference type="OrthoDB" id="168808at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056433">
        <property type="entry name" value="DmsR-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007050">
        <property type="entry name" value="HTH_bacterioopsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236:SF1">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24277">
        <property type="entry name" value="DmsR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04967">
        <property type="entry name" value="HTH_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="175"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="209" mass="22701" checksum="C595A03D5997D94F" modified="2017-05-10" version="1">MGLEAAVKIPNPEQCPVADVSAEADARVGDVSWSGVPNDDGSVVEEFELDTDADPPDDMEKVFGDNRYSIYRFDRAGDAPCICQRIETFGCPVADVEAVDGSLRIVFRPADVESLQEIVGTLQREFGGIQLEHLVQSSEPTEGETVVVDRGRLTDRQREVLETAYEMGYFEHPKGANAGEVADELGINSATLRGHLSAAQSKLLDEMLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV41</accession>
    <name>A0A1U7EV41_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7511 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_1552A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV41"/>
    <dbReference type="STRING" id="348780.NP_1552A"/>
    <dbReference type="EnsemblBacteria" id="CAI48867">
        <property type="protein sequence ID" value="CAI48867"/>
        <property type="gene ID" value="NP_1552A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_1552A"/>
    <dbReference type="eggNOG" id="arCOG07586">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_207593_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055933">
        <property type="entry name" value="DUF7511"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24351">
        <property type="entry name" value="DUF7511"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7511" evidence="2">
        <location>
            <begin position="21"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="66" mass="7334" checksum="A09938C072797337" modified="2017-05-10" version="1">MTDMSSSQASGTDEAMRPHTLEAIPADADDACGEYTFVPRDAEGDERLTRWLTAEEDAIVDLSEWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV93</accession>
    <name>A0A1U7EV93_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_1668A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RelA/SpoT family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322558.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV93"/>
    <dbReference type="STRING" id="348780.NP_1668A"/>
    <dbReference type="EnsemblBacteria" id="CAI48925">
        <property type="protein sequence ID" value="CAI48925"/>
        <property type="gene ID" value="NP_1668A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702741"/>
    <dbReference type="KEGG" id="nph:NP_1668A"/>
    <dbReference type="eggNOG" id="arCOG00357">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037276_1_0_2"/>
    <dbReference type="OrthoDB" id="5875at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01669">
        <property type="entry name" value="TGS_MJ1332_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01899">
        <property type="entry name" value="Ygr210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000002">
        <property type="entry name" value="GTP pyrophosphokinase (RelA/SpoT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013646">
        <property type="entry name" value="YGR210-like_G4"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007171">
        <property type="entry name" value="PRK09602.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305">
        <property type="entry name" value="OBG GTPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305:SF1">
        <property type="entry name" value="OBG-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08438">
        <property type="entry name" value="YGR210-like_G4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="3">
        <location>
            <begin position="2"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="393" mass="42614" checksum="9D82CE962DEC91A7" modified="2017-05-10" version="1">MLSVALAGKPNAGKSTFYTAATMADADTGNYPFTTIDPNRGVTHVRTRCPCLDLESRCGNDTCHGGKRYVPVELLDVAGLVPGAHEGKGLGNQFLDELTNADVILHVVDASGGTDAEGEPVEAGSYDPLEDVEFIETEMDLWLADIVERNWETIERQARSPEFDFEAALTELLTGVGASEYDVMAVLRELEYETDPGAWSDADREELARLLRERTKPIVVVANKADIAPEENIERLREAAERVIPCTAEGELALRRGVEAGVVDYDPGDEAFEIAAEVSEAQRQGLESVRSVIDQWGGTGVQEALDEAVYGLLDQITVYPVQNETRWTDGQGDVLPDAFLLPSGATPKDLAFAVHSDIGEGYLHAVDAREERRIGEGHELAEGDVIKIVSTAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVB2</accession>
    <name>A0A1U7EVB2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">UbiB family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_1742A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVB2"/>
    <dbReference type="STRING" id="348780.NP_1742A"/>
    <dbReference type="EnsemblBacteria" id="CAI48962">
        <property type="protein sequence ID" value="CAI48962"/>
        <property type="gene ID" value="NP_1742A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_1742A"/>
    <dbReference type="eggNOG" id="arCOG01189">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006533_0_3_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd05121">
        <property type="entry name" value="ABC1_ADCK3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050154">
        <property type="entry name" value="UbiB_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10566">
        <property type="entry name" value="CHAPERONE-ACTIVITY OF BC1 COMPLEX CABC1 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10566:SF113">
        <property type="entry name" value="PROTEIN ACTIVITY OF BC1 COMPLEX KINASE 7, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="485"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="510"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="3">
        <location>
            <begin position="85"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="552" mass="62473" checksum="B5042D0E4A7E3719" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVC0</accession>
    <name>A0A1U7EVC0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DUF2240 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1728A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322588.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVC0"/>
    <dbReference type="STRING" id="348780.NP_1728A"/>
    <dbReference type="EnsemblBacteria" id="CAI48955">
        <property type="protein sequence ID" value="CAI48955"/>
        <property type="gene ID" value="NP_1728A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701240"/>
    <dbReference type="KEGG" id="nph:NP_1728A"/>
    <dbReference type="eggNOG" id="arCOG04418">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134817_0_0_2"/>
    <dbReference type="OrthoDB" id="146786at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018716">
        <property type="entry name" value="DUF2240"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09999">
        <property type="entry name" value="DUF2240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="146" mass="15740" checksum="351DE953999C10C7" modified="2017-05-10" version="1">MSLRTAVAAPFREEGARKMGESAFVVALSLDRDWFSPDQAKRLVDVAASEGLLERADGDLVASFDPASTTVPEDFAPDESLLQERSTFERVLDELVESGTEKQDAVAGINQLQSELGVTIEAAAVLYAHEHGLDVDDYAATARAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVD6</accession>
    <name>A0A1U7EVD6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF460 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1786A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322617.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVD6"/>
    <dbReference type="STRING" id="348780.NP_1786A"/>
    <dbReference type="EnsemblBacteria" id="CAI48984">
        <property type="protein sequence ID" value="CAI48984"/>
        <property type="gene ID" value="NP_1786A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701253"/>
    <dbReference type="KEGG" id="nph:NP_1786A"/>
    <dbReference type="eggNOG" id="arCOG03142">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1080186_0_0_2"/>
    <dbReference type="OrthoDB" id="17710at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="244" mass="25717" checksum="5D1DC4402AB7AA68" modified="2017-05-10" version="1">MIVVATADFEVYHGVVDELGDRDVPFTTVEPDEELPDGAEVAIVGPEDEHPAIETVRANPGDPRRAVEEAVALLRGDGGRTVVGVDPGERPGIAVLVGGTVVAAFQVPAADAPAVVARETEDAADPLVRVGDGARIKGAKIIDELEDIPVELVDETGTTPYLGTGARGMGDVLAAVNIARIEGERVSSRDIEPTAGELQRIKDRSRAESDDDRAITEALARRVANGDLTIAEALRRHRQQENGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVE8</accession>
    <name>A0A1U7EVE8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Sensor box histidine kinase</fullName>
            <ecNumber evidence="10">2.7.13.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">NP_1804A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.13.3" evidence="10"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48993.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322625.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVE8"/>
    <dbReference type="EnsemblBacteria" id="CAI48993">
        <property type="protein sequence ID" value="CAI48993"/>
        <property type="gene ID" value="NP_1804A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701259"/>
    <dbReference type="KEGG" id="nph:NP_1804A"/>
    <dbReference type="eggNOG" id="arCOG02364">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_58_2"/>
    <dbReference type="OrthoDB" id="230688at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004673">
        <property type="term" value="F:protein histidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00075">
        <property type="entry name" value="HATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43065:SF10">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43065">
        <property type="entry name" value="SENSOR HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="PAS" evidence="8">
        <location>
            <begin position="159"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="9">
        <location>
            <begin position="230"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="298"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI48993.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="499" mass="56231" checksum="4A9FF83D205C65E9" modified="2017-05-10" version="1">MSGGLADTADGIDPEVAETTVQLLVDDDERRRRLDDVLGKRYRILADEATPATEADLHVVDSAALETLQDQLARLTRRDQPVFRPVLLLCQSGCRCDVAEEALHEDETETDEPPVVDDVIRPSLDDVLLRRRIRTLVVRRRQSRRLESKVSTLRQRERRLRSFKRAVERSGHSIMITDTEGVIRYVNPAFEGITGYSEAEAVGEKPSLLQSGEHDETFYRSLWTTISDGDVWESQVINERKDGEQYVVDQTIAPITDGDRIEGYVSVNSDITDRVTRERELRSRERELDRLRQILTRVLRHNIRNDATVFTGYGELIAENADEPYASMAEGIVETAESLSKTTEKARELSRLVERDPTPTAHDLSSLLRSTAEEVQTEFPEVDITVDVDDDLSVLGTEELPRVFHALLENAAAHNDASDPEVRLRTVAGDPVLVVIEDNGPGIPETEVKSLDSREETPLQHSDGTGLWLAGWILDRSDGTLRFDDHDDGTRIEIELLRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVN9</accession>
    <name>A0A1U7EVN9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Amidohydrolase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2032A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322736.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVN9"/>
    <dbReference type="STRING" id="348780.NP_2032A"/>
    <dbReference type="EnsemblBacteria" id="CAI49107">
        <property type="protein sequence ID" value="CAI49107"/>
        <property type="gene ID" value="NP_2032A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702787"/>
    <dbReference type="KEGG" id="nph:NP_2032A"/>
    <dbReference type="eggNOG" id="arCOG00692">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012358_1_0_2"/>
    <dbReference type="OrthoDB" id="42910at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01305">
        <property type="entry name" value="archeal_chlorohydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050287">
        <property type="entry name" value="MTA/SAH_deaminase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43794">
        <property type="entry name" value="AMINOHYDROLASE SSNA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43794:SF5">
        <property type="entry name" value="CHLOROHYDROLASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="1">
        <location>
            <begin position="40"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Amidohydrolase-related" evidence="1">
        <location>
            <begin position="197"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="338" mass="35445" checksum="B1C91ED2C7BBC81D" modified="2017-05-10" version="1">MELSGTILAGPDYEPIEGRVVVEDGEITAIEEVETASSDIICPAFVNAHTHIGDSIAKEAGRGLSLDELVAPPDGLKHRLLDDASREELVAAMARSLQFMQAAGTGACIEFREGGVEGIEALQAATAGLDIESVALGRDTTDVLDIADGYGASGARDGDFSAERTATGRAGLLFGIHAGERDAHDINPALDLEPDFLVHMVHAEPLHLERVADRGTPVVVCPRSNLVTDVGVPPVADLLERTTVALGTDNVLLNSPSMFREMAVTAKLCDVTAPEVLRMATAAGAEIAGLNCGVIEPGRRARLLVLDGDSDNLAGVRDPVRAVVRRAGVNDVKNVVLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVS0</accession>
    <name>A0A1U7EVS0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2148A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVS0"/>
    <dbReference type="STRING" id="348780.NP_2148A"/>
    <dbReference type="EnsemblBacteria" id="CAI49165">
        <property type="protein sequence ID" value="CAI49165"/>
        <property type="gene ID" value="NP_2148A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_2148A"/>
    <dbReference type="HOGENOM" id="CLU_2433954_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="90" mass="10129" checksum="B0299EE8D6B2747E" modified="2017-05-10" version="1">MRPHRPVQTVKTRGRTDAYTGPEPQGSYMDGRRVWVPVEDEPRLGTVVEYTYAPKTGAPLLAVELDEPTEDTERVVVNPETDDVLFESTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW12</accession>
    <name>A0A1U7EW12_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">SWIM zinc finger domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_2328A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322882.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW12"/>
    <dbReference type="STRING" id="348780.NP_2328A"/>
    <dbReference type="EnsemblBacteria" id="CAI49255">
        <property type="protein sequence ID" value="CAI49255"/>
        <property type="gene ID" value="NP_2328A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701407"/>
    <dbReference type="KEGG" id="nph:NP_2328A"/>
    <dbReference type="eggNOG" id="arCOG09044">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1998809_0_0_2"/>
    <dbReference type="OrthoDB" id="318346at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04434">
        <property type="entry name" value="SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="3">
        <location>
            <begin position="47"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="101"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="124" mass="13873" checksum="D5C2851958023FE6" modified="2017-05-10" version="1">MTSKLDFEAERDDGSESWDRSDPLNAVICRMSWREWAVALPDGDEAHICELHHDGRGYQGRCDCQGFKFHSGPCAHLIALRKADALGLHDARGDRIELASDDRRHADDIEDAVDRAATDGGRNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW24</accession>
    <name>A0A1U7EW24_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">CobW domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">NP_2376A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SIMIBI class G3E GTPase family. ZNG1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322905.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW24"/>
    <dbReference type="STRING" id="348780.NP_2376A"/>
    <dbReference type="EnsemblBacteria" id="CAI49279">
        <property type="protein sequence ID" value="CAI49279"/>
        <property type="gene ID" value="NP_2376A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701429"/>
    <dbReference type="KEGG" id="nph:NP_2376A"/>
    <dbReference type="eggNOG" id="arCOG01233">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017452_2_0_2"/>
    <dbReference type="OrthoDB" id="359387at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03112">
        <property type="entry name" value="CobW-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1220.10">
        <property type="entry name" value="CobW-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036627">
        <property type="entry name" value="CobW-likC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011629">
        <property type="entry name" value="CobW-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051927">
        <property type="entry name" value="Zn_Chap_cDPG_Synth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43603">
        <property type="entry name" value="COBW DOMAIN-CONTAINING PROTEIN DDB_G0274527"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43603:SF1">
        <property type="entry name" value="ZINC-REGULATED GTPASE METALLOPROTEIN ACTIVATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07683">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00833">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobW C-terminal" evidence="7">
        <location>
            <begin position="263"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="389"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="391"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="411"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="428" mass="47028" checksum="55CF049D60CAFEC2" modified="2017-05-10" version="1">MSEQSTPVTVLSGTLGAGKTTTLNHLLRESGGRELAVLVNDMGEVNVDAELVAESSDISAEEEELVELSNGCICCELRGDLLDAIGALTSERSYDGIVVESTGVAEPLPVAQTLTLGFDQSDLDPTEFYEETGIEPLENCHLDTTVTVVDAHQFQSAMESDEILDDDGTEKHLGDLLVEQVEFCDVLVLNKCDLVDEATLDEIEAVLETLQPRAEIIRTTHGEVDVDTVVDTGRFDFDEARQSAGWLQELQEPHASAEEEHGVGSFVFEARRPFHPERFAELLDDLPSSVVRSKGHFWLAGREELAMSLNVAGESIRIAPGGRWIATLPPEERAEQFEAHPELESMWDDEWGDRGTQLVVIGTEMDATSLEDRLADCLLTDEDMEADWSAFEDRFPTFERPEAEPEKADADEAEQAGTDDSEEIGLAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW51</accession>
    <name>A0A1U7EW51_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">PspA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">pspA</name>
        <name evidence="3" type="ordered locus">NP_2412A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PspA/Vipp/IM30 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322923.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW51"/>
    <dbReference type="STRING" id="348780.NP_2412A"/>
    <dbReference type="EnsemblBacteria" id="CAI49297">
        <property type="protein sequence ID" value="CAI49297"/>
        <property type="gene ID" value="NP_2412A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703064"/>
    <dbReference type="KEGG" id="nph:NP_2412A"/>
    <dbReference type="eggNOG" id="arCOG04782">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056466_1_0_2"/>
    <dbReference type="OrthoDB" id="146015at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007157">
        <property type="entry name" value="PspA_VIPP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31088">
        <property type="entry name" value="MEMBRANE-ASSOCIATED PROTEIN VIPP1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31088:SF6">
        <property type="entry name" value="PHAGE SHOCK PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04012">
        <property type="entry name" value="PspA_IM30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="174"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="232"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00043985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="268" mass="30487" checksum="191560885775E059" modified="2017-05-10" version="1">MGILSRASYVIRSKINAVLNRAEDPHETLDYSYEQLRDELQDVKQGIADLTTQKKRLEIQKNRLEENVEKHNEQARQAVEQGRDDLARRALEKKKQKMQQIEDLETQIADLQNTQDNLVEKKNELQNRIQEFRTEKETMKARYEAAEASARVSEAMTGAGEEMENINRSIERARDDTEDMEARAAAMDELEESGAFDSALSNESDLEAELEEVRQEGEVEAELETLKADAGVESDGEADPEVELEETNEAAADPEIEAELDDLKKDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW79</accession>
    <name>A0A1U7EW79_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">TrmB family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_2518A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322976.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW79"/>
    <dbReference type="STRING" id="348780.NP_2518A"/>
    <dbReference type="EnsemblBacteria" id="CAI49350">
        <property type="protein sequence ID" value="CAI49350"/>
        <property type="gene ID" value="NP_2518A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701472"/>
    <dbReference type="KEGG" id="nph:NP_2518A"/>
    <dbReference type="eggNOG" id="arCOG02037">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072493_0_0_2"/>
    <dbReference type="OrthoDB" id="202962at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055859">
        <property type="entry name" value="DUF7436"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051797">
        <property type="entry name" value="TrmB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293:SF1">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24217">
        <property type="entry name" value="DUF7436"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator TrmB N-terminal" evidence="2">
        <location>
            <begin position="4"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7436" evidence="3">
        <location>
            <begin position="109"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="72"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="268" mass="30479" checksum="1B070F2B869ACA9D" modified="2017-05-10" version="1">MASLRDLGLSEYEARAYRALLKTGPTTAKELSRVSDVPMGRIYDVLNSIEQYNLVRSQSASRPKKYVAVEPSTALDRLLEDKKRELDEKANQYESIVDELSGELETAEPVEDTFWTAAVGPDETVDLLMERISAADSEVVMVLSSYIEQFFDIDTVGTLILDELTDAMDRGVDVRLLMRPEVMSVLPESIGERYRTSLTENERFTVCISEDVTGTFTLIDDNEVTIEVPHPLKNREVFAMIDLKDREFAQSVRSEFEPRWEQAEELTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW93</accession>
    <name>A0A1U7EW93_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">NP_2516A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322975.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW93"/>
    <dbReference type="STRING" id="348780.NP_2516A"/>
    <dbReference type="EnsemblBacteria" id="CAI49349">
        <property type="protein sequence ID" value="CAI49349"/>
        <property type="gene ID" value="NP_2516A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701471"/>
    <dbReference type="KEGG" id="nph:NP_2516A"/>
    <dbReference type="eggNOG" id="arCOG02329">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02365">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_58_2"/>
    <dbReference type="OrthoDB" id="230688at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004673">
        <property type="term" value="F:protein histidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00075">
        <property type="entry name" value="HATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052162">
        <property type="entry name" value="Sensor_kinase/Photoreceptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43304:SF1">
        <property type="entry name" value="PAC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43304">
        <property type="entry name" value="PHYTOCHROME-LIKE PROTEIN CPH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="7">
        <location>
            <begin position="2"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="8">
        <location>
            <begin position="77"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="7">
        <location>
            <begin position="124"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="6">
        <location>
            <begin position="258"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI49349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="464" mass="51915" checksum="7FBDFA6188FDE213" modified="2017-05-10" version="1">MDAETLASLLDHSQAKIVVIDEAGTYQYANDAVSRLLGYDPDAFVGLNTFECMHPDDRPEMEARFESLRSAPEGASETVEYRHRSADGGWVWLETRLSNRKLPDFDGYVASSHDISERKRAERQRNELRTRLEELTANTNDVLWTVSADWDEMLFVNDAYEDIWGQPTAEVRENPERFLDGIRPKDRSKARDGMERLAGGESVEMEYWVDPSVSFRRRVWVRAEPIFEDGDVVRIVGTARDVTDRYRRQRQLRVLDDLLRHNIRNTMNIVLGHAELIQRGAHPDAPSGMGTVIDSATDLLNTAEKGREVVEVLSGANETVTVDIADCVSTAVENARERYPEVPIKLRQPSTAVAEAIPKINQAVAELLENAIQHADGAAEVTAVVECQGERITINIIDNGPPIPENEIRPLFTEADLSAINHGTGLGLWLVYWVADLSDGELTFGRRADGEGNVVTLSLPQAER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWH7</accession>
    <name>A0A1U7EWH7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Methylmalonyl-CoA mutase subunit B (Cobalamin-binding subunit)</fullName>
            <ecNumber evidence="8">5.4.99.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">mmcB</name>
        <name evidence="8" type="ordered locus">NP_2710A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>adenosylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methylamine corrinoid protein family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.2" evidence="8"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49446.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939548.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWH7"/>
    <dbReference type="STRING" id="348780.NP_2710A"/>
    <dbReference type="EnsemblBacteria" id="CAI49446">
        <property type="protein sequence ID" value="CAI49446"/>
        <property type="gene ID" value="NP_2710A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702902"/>
    <dbReference type="KEGG" id="nph:NP_2710A"/>
    <dbReference type="eggNOG" id="arCOG01710">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128233_0_0_2"/>
    <dbReference type="OrthoDB" id="9041at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004494">
        <property type="term" value="F:methylmalonyl-CoA mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02071">
        <property type="entry name" value="MM_CoA_mut_B12_BD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.280">
        <property type="entry name" value="Cobalamin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006159">
        <property type="entry name" value="Acid_CoA_mut_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036724">
        <property type="entry name" value="Cobalamin-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00640">
        <property type="entry name" value="acid_CoA_mut_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48101:SF1">
        <property type="entry name" value="METHYLMALONYL-COA MUTASE, LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48101">
        <property type="entry name" value="METHYLMALONYL-COA MUTASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02310">
        <property type="entry name" value="B12-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52242">
        <property type="entry name" value="Cobalamin (vitamin B12)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51332">
        <property type="entry name" value="B12_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0846">Cobalamin</keyword>
    <keyword evidence="6" id="KW-0170">Cobalt</keyword>
    <keyword evidence="5 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B12-binding" evidence="7">
        <location>
            <begin position="9"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49446.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="138" mass="14966" checksum="1CC9D292451DACC1" modified="2017-05-10" version="1">MSTEGEQRTIRCLVAKVGLDGHDRGAHVIARAFRDAGFEVIYSGLHNSPDEIVQAAVQEDVDVLGISILSGAHDTLVPKILDGLKEYDAFEDTLVITGGIIPDEDHDELYDAGVDEIFGPGASMEETITFVRENAPER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWM4</accession>
    <name>A0A1U7EWM4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Monooxygenase (Homolog to alkanesulfonate monooxygenase)</fullName>
            <ecNumber evidence="3">1.14.14.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2804A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To bacterial alkanal monooxygenase alpha and beta chains.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.-" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323118.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWM4"/>
    <dbReference type="STRING" id="348780.NP_2804A"/>
    <dbReference type="EnsemblBacteria" id="CAI49493">
        <property type="protein sequence ID" value="CAI49493"/>
        <property type="gene ID" value="NP_2804A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701563"/>
    <dbReference type="KEGG" id="nph:NP_2804A"/>
    <dbReference type="eggNOG" id="arCOG02410">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027853_9_0_2"/>
    <dbReference type="OrthoDB" id="167712at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00347">
        <property type="entry name" value="Flavin_utilizing_monoxygenases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="entry name" value="Luciferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050766">
        <property type="entry name" value="Bact_Lucif_Oxidored"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019949">
        <property type="entry name" value="CmoO-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03558">
        <property type="entry name" value="oxido_grp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30137">
        <property type="entry name" value="LUCIFERASE-LIKE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30137:SF6">
        <property type="entry name" value="LUCIFERASE-LIKE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="Bacterial luciferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Luciferase-like" evidence="2">
        <location>
            <begin position="2"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="339" mass="35700" checksum="19F1402008BFE3A2" modified="2017-05-10" version="1">MNLSAVDLSPVPDDGTATEAYADTVEAAEQAERLGLTRYWVAEHHGMADTLAGTTPEVLLGRLAAATDSIRLGSGAVLLNHYSPLKVAEIFGALDGLAPGRVDAGLGRANGSPAVDRALGTERHVRNPDEDHAEKIEAVVAHLYDDFPDGHEYADITIPRSGAGPAVPWVLGSSPSSAAVAGRLGLRYCFAAFIRPQFMPRAFETYREEFEASPLAGGVDEPQAMVAVNAVCAPTDEAAARQRSVAEAVYARMQRGTVGARPSVETAIDELGGVPDPTPTSLDIDAWPRAISGSPETVAALFDQLADELGVDEVMVQQTVGDHETKLRSHELLADAVGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWN1</accession>
    <name>A0A1U7EWN1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7960 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2814A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323123.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWN1"/>
    <dbReference type="STRING" id="348780.NP_2814A"/>
    <dbReference type="EnsemblBacteria" id="CAI49498">
        <property type="protein sequence ID" value="CAI49498"/>
        <property type="gene ID" value="NP_2814A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701567"/>
    <dbReference type="KEGG" id="nph:NP_2814A"/>
    <dbReference type="eggNOG" id="arCOG04571">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149759_0_0_2"/>
    <dbReference type="OrthoDB" id="159011at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058266">
        <property type="entry name" value="DUF7960"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25901">
        <property type="entry name" value="DUF7960"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7960" evidence="1">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="152" mass="17295" checksum="AF530096FD826802" modified="2017-05-10" version="1">MYTGRTEKPCCLCGRPEPDHRIDIPPRAIQLLKHSGPIAWQDIEGAVSLYFCEDDWQTVKDLVLETGMSPLSRCNAGRASFVLREDFEALLNDVRDEPDQRPLERELREKAESAVDRYEDGDDLVEARDLVEARVVLWALADLDTRQPQADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWS5</accession>
    <name>A0A1U7EWS5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2932A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323181.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWS5"/>
    <dbReference type="STRING" id="348780.NP_2932A"/>
    <dbReference type="EnsemblBacteria" id="CAI49557">
        <property type="protein sequence ID" value="CAI49557"/>
        <property type="gene ID" value="NP_2932A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701602"/>
    <dbReference type="KEGG" id="nph:NP_2932A"/>
    <dbReference type="HOGENOM" id="CLU_2784215_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="68" mass="7453" checksum="4EB8E3EBD4ACFA08" modified="2017-05-10" version="1">MVERRDDPYRRDAGNRRRRRRWAKVGITVLVGLSAGLMTTQGEASLAVVLWAIGGGLLAGAALAWYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWV5</accession>
    <name>A0A1U7EWV5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alpha-IPM synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">leuA3</name>
        <name evidence="4" type="ordered locus">NP_2994A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323212.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWV5"/>
    <dbReference type="STRING" id="348780.NP_2994A"/>
    <dbReference type="EnsemblBacteria" id="CAI49588">
        <property type="protein sequence ID" value="CAI49588"/>
        <property type="gene ID" value="NP_2994A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702878"/>
    <dbReference type="KEGG" id="nph:NP_2994A"/>
    <dbReference type="eggNOG" id="arCOG02092">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022158_4_0_2"/>
    <dbReference type="OrthoDB" id="6555at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003852">
        <property type="term" value="F:2-isopropylmalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050073">
        <property type="entry name" value="2-IPM_HCS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10277:SF9">
        <property type="entry name" value="2-ISOPROPYLMALATE SYNTHASE 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10277">
        <property type="entry name" value="HOMOCITRATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="296" mass="30058" checksum="ADF53E5C9E9D9680" modified="2017-05-10" version="1">MRLCDATVDEAARLPDRTYTADQRVEAGRALDRLGVPLVRAGRPAHDETDAEVVRRLADTLSADTVAVAPPDYEAVEDALETEADIIDVFVPVAGPRLEAAFGGSSEAALDAAEDAVLRAREGGATAHLTLIDAFRAEIPAIAGVFGRFDCHIVLDDASGGRTPPFVAGFLRTLADASADLTRAGVRFRDDVGCGTANAIVAAETGIDRVDASVAGVGARAGLAATEELVVATAAGGGDAGVTTEAAMPAFEAVCSALDIQVGDRKAILGDAATATAPTADDVAPSAFDPADFGGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX06</accession>
    <name>A0A1U7EX06_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cysteine synthase</fullName>
            <ecNumber evidence="8">2.5.1.47</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cysK2</name>
        <name evidence="8" type="ordered locus">NP_3116A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.47" evidence="8"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323271.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX06"/>
    <dbReference type="STRING" id="348780.NP_3116A"/>
    <dbReference type="EnsemblBacteria" id="CAI49649">
        <property type="protein sequence ID" value="CAI49649"/>
        <property type="gene ID" value="NP_3116A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702654"/>
    <dbReference type="KEGG" id="nph:NP_3116A"/>
    <dbReference type="eggNOG" id="arCOG01430">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021018_1_0_2"/>
    <dbReference type="OrthoDB" id="10138at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004124">
        <property type="term" value="F:cysteine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01561">
        <property type="entry name" value="CBS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000006">
        <property type="entry name" value="Cysteine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="7">
        <location>
            <begin position="11"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="327" mass="34481" checksum="67FBA5FA250C161F" modified="2017-05-10" version="1">MKRGILDTIGSPLVQVESPPGSVIAAKIESKNPGGSAKDRPALAMVEAAEAAGELEPGDRIVEPTSGNTGIGLAVVAAAKGYDLTVVMPESKSPERRALMRAYGADLELVEGTISDAKDCADELEAEGMVQIRQFENPANPEAHYRTTGEEILRQVGDRDIDALVAGIGTGGTISGTGRRLKEVFPEMEVIGVEPEGNAVLSGDEPGDDDFQGMGPGFVAPNLDRELLDGVEVVDIETAEAECRRLAEEEGVLVGQSSGGSLVAAKRVARRLADERGIEVPCPGPVNDIGLLDDEPPERQAVPDDCPLVVTVFWDSGERYMSTGMFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX22</accession>
    <name>A0A1U7EX22_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">P-type transport ATPase (Probable substrate copper/metal cation)</fullName>
            <ecNumber evidence="12">3.6.3.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">tpa03</name>
        <name evidence="12" type="ordered locus">NP_3140A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.-" evidence="12"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323283.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX22"/>
    <dbReference type="STRING" id="348780.NP_3140A"/>
    <dbReference type="EnsemblBacteria" id="CAI49661">
        <property type="protein sequence ID" value="CAI49661"/>
        <property type="gene ID" value="NP_3140A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703350"/>
    <dbReference type="KEGG" id="nph:NP_3140A"/>
    <dbReference type="eggNOG" id="arCOG01576">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001771_0_3_2"/>
    <dbReference type="OrthoDB" id="8588at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043682">
        <property type="term" value="F:P-type divalent copper transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055070">
        <property type="term" value="P:copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01511">
        <property type="entry name" value="ATPase-IB1_Cu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520">
        <property type="entry name" value="ATP7, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520:SF8">
        <property type="entry name" value="P-TYPE CU(+) TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="152"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="188"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="223"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="252"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="404"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="433"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="738"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="11">
        <location>
            <begin position="65"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI49661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="782" mass="83305" checksum="5EF134225D29AF9F" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX27</accession>
    <name>A0A1U7EX27_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Indole-3-glycerol phosphate synthase</fullName>
            <shortName evidence="11">IGPS</shortName>
            <ecNumber evidence="4 11">4.1.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11 13" type="primary">trpC</name>
        <name evidence="13" type="ordered locus">NP_3166A</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H(+) = (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:23476"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="EC" id="4.1.1.48"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the TrpC family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.48" evidence="4 11"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323296.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX27"/>
    <dbReference type="STRING" id="348780.NP_3166A"/>
    <dbReference type="EnsemblBacteria" id="CAI49674">
        <property type="protein sequence ID" value="CAI49674"/>
        <property type="gene ID" value="NP_3166A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703410"/>
    <dbReference type="KEGG" id="nph:NP_3166A"/>
    <dbReference type="eggNOG" id="arCOG01088">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034247_0_1_2"/>
    <dbReference type="OrthoDB" id="15223at2157"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00043"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004425">
        <property type="term" value="F:indole-3-glycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004640">
        <property type="term" value="F:phosphoribosylanthranilate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00331">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00134_A">
        <property type="entry name" value="IGPS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054875">
        <property type="entry name" value="Indglycph_syn_Halo_TrpC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045186">
        <property type="entry name" value="Indole-3-glycerol_P_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013798">
        <property type="entry name" value="Indole-3-glycerol_P_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001468">
        <property type="entry name" value="Indole-3-GlycerolPSynthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041303">
        <property type="entry name" value="Indglycph_syn_Halo_TrpC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22854:SF2">
        <property type="entry name" value="INDOLE-3-GLYCEROL-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22854">
        <property type="entry name" value="TRYPTOPHAN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00218">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00614">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="7 11" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="10 11 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Indole-3-glycerol phosphate synthase" evidence="12">
        <location>
            <begin position="26"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAI49674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="254" mass="26763" checksum="1919EDA24EBB426E" modified="2017-05-10" version="1">MDTSGELSPAVRSILSAAEERDGGETRCTVDARSLPDALDAAADGGRAPVIAELKPTSPTTDGERTDDPVELAEEMVAGGAAALSVLTEPEHFGGSTETLRRVREAVSVPVLRKDFLLREAQLDAVASDVVLLIARFVDDLDGLLAAARERGFQVLVEVHTPGEARTALDAGADIIGVNNRDLAELSVDLSTFESVVESVPELRDGSVTLIAESGIETREDVTRMRSAGADGLLVGSAIMDGDVRENTERLTDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX55</accession>
    <name>A0A1U7EX55_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Phospholipase D domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">NP_3226A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49704.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX55"/>
    <dbReference type="STRING" id="348780.NP_3226A"/>
    <dbReference type="EnsemblBacteria" id="CAI49704">
        <property type="protein sequence ID" value="CAI49704"/>
        <property type="gene ID" value="NP_3226A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3226A"/>
    <dbReference type="eggNOG" id="arCOG02039">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026762_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016891">
        <property type="term" value="F:RNA endonuclease activity producing 5'-phosphomonoesters, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09128">
        <property type="entry name" value="PLDc_unchar1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051406">
        <property type="entry name" value="PLD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43856">
        <property type="entry name" value="CARDIOLIPIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43856:SF1">
        <property type="entry name" value="MITOCHONDRIAL CARDIOLIPIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="512"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="5">
        <location>
            <begin position="447"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI49704.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="548" mass="58789" checksum="D3121B542158DA15" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXB3</accession>
    <name>A0A1U7EXB3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC-type transport system permease protein (Probable substrate branched-chain amino acids)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">livH4</name>
        <name evidence="10" type="synonym">abc08p1</name>
        <name evidence="10" type="ordered locus">NP_3426A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323424.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXB3"/>
    <dbReference type="STRING" id="348780.NP_3426A"/>
    <dbReference type="EnsemblBacteria" id="CAI49804">
        <property type="protein sequence ID" value="CAI49804"/>
        <property type="gene ID" value="NP_3426A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702389"/>
    <dbReference type="KEGG" id="nph:NP_3426A"/>
    <dbReference type="eggNOG" id="arCOG01270">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039929_2_1_2"/>
    <dbReference type="OrthoDB" id="43815at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06582">
        <property type="entry name" value="TM_PBP1_LivH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052157">
        <property type="entry name" value="BCAA_transport_permease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11795:SF442">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11795">
        <property type="entry name" value="BRANCHED-CHAIN AMINO ACID TRANSPORT SYSTEM PERMEASE PROTEIN LIVH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="238"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI49804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="304" mass="32475" checksum="335F44D89C9D2DFF" modified="2017-05-10" version="1">MITILSSILGPTFNGLYFAALLFFASVGLTLVFGVLNLLNMAHAAFFGLGVYIGVWTLNNMHDISTSLAVLLVLIVIAIPVVVSVLAGALDKTLFAPLYDIEPVYQLLATFGVLLMIEDLIKFIWGTTPIAVDSAVNPSRQLGSFEVLGSSLSMYRTFIVLMFIVTAVGLFLMFERTKLGKISLAMAEDYEMVQMLGINVNRVRVLIFSISVGLAALGGALFVPLAPATPAITLEYVLLSFAVIVIGGLGSLKGAIVASLIIGLVRSFGITYVPQIELAIVFLLMAAVIIVKPEGLFGHEEVGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXB5</accession>
    <name>A0A1U7EXB5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF35 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3440A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323431.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXB5"/>
    <dbReference type="STRING" id="348780.NP_3440A"/>
    <dbReference type="EnsemblBacteria" id="CAI49811">
        <property type="protein sequence ID" value="CAI49811"/>
        <property type="gene ID" value="NP_3440A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701773"/>
    <dbReference type="KEGG" id="nph:NP_3440A"/>
    <dbReference type="eggNOG" id="arCOG01286">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119412_0_0_2"/>
    <dbReference type="OrthoDB" id="9573at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002878">
        <property type="entry name" value="ChsH2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052513">
        <property type="entry name" value="Thioester_dehydratase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34075">
        <property type="entry name" value="BLR3430 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34075:SF5">
        <property type="entry name" value="BLR3430 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01796">
        <property type="entry name" value="OB_ChsH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ChsH2 C-terminal OB-fold" evidence="1">
        <location>
            <begin position="45"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="121" mass="12870" checksum="9AF0DAAE67326C43" modified="2017-05-10" version="1">MNYDEFLDALAAGDERYLESPEGHGWLPPRRVCPESGSTDLSPAALPEEGEIVALTQVHVPAPDFADDAPYCTAVVDFGPVRVTGVLQGVEYEAVAIGDTVTAGVGHSETTDERIVVFEPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXD8</accession>
    <name>A0A1U7EXD8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Receiver box histidine kinase</fullName>
            <ecNumber evidence="5">2.7.13.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_3458A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.13.3" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323440.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXD8"/>
    <dbReference type="STRING" id="348780.NP_3458A"/>
    <dbReference type="EnsemblBacteria" id="CAI49820">
        <property type="protein sequence ID" value="CAI49820"/>
        <property type="gene ID" value="NP_3458A"/>
    </dbReference>
    <dbReference type="GeneID" id="32155012"/>
    <dbReference type="KEGG" id="nph:NP_3458A"/>
    <dbReference type="eggNOG" id="arCOG06219">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_72_2"/>
    <dbReference type="OrthoDB" id="3369at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00156">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43547:SF2">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43547">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="28"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="3">
        <location>
            <begin position="177"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="385" mass="42670" checksum="D613236CBA7320A1" modified="2017-05-10" version="1">MPESYAAVMAADGSENPPWKDLPDSTPSVLVVDDSEFFADMMASEIENGSSFETISCYDGETALEKIERNDIACIVSDYRMDPVDGIELLETVRDEYGAIPFIILTGQGGEEVAIEAIRLGATDYVTKEMIIEGEEFSLLLNRIEQSVNHTMAQTELARRKELIETQRDNLEFLNEVLRHDIRNDLQIISAYADRLEDHVDQAGRADLETIQQSTDNAIELTKTTDKVAEIMLRQEVDRQAVSIRDIITEEIETIRAADPNATVEVVGEIPDVEVLAGQMIDAVFRNLLKNAVQHNDKREPEITVSATEQEEVLEVRVADNGPGISEELQQSIFGKGERGLESEGTGIGLHLVATLVDDYDGDVRIEDNDPEGSVFVVELQLADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXF6</accession>
    <name>A0A1U7EXF6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">HypE family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hypE1</name>
        <name evidence="4" type="ordered locus">NP_3548A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HypE family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323485.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXF6"/>
    <dbReference type="STRING" id="348780.NP_3548A"/>
    <dbReference type="EnsemblBacteria" id="CAI49865">
        <property type="protein sequence ID" value="CAI49865"/>
        <property type="gene ID" value="NP_3548A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702852"/>
    <dbReference type="KEGG" id="nph:NP_3548A"/>
    <dbReference type="eggNOG" id="arCOG00636">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041631_0_0_2"/>
    <dbReference type="OrthoDB" id="31494at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06061">
        <property type="entry name" value="PurM-like1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011854">
        <property type="entry name" value="HypE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30303:SF4">
        <property type="entry name" value="HYDROGENASE EXPRESSION_FORMATION PROTEIN HYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30303">
        <property type="entry name" value="HYDROGENASE ISOENZYMES FORMATION PROTEIN HYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005644">
        <property type="entry name" value="Hdrgns_mtr_HypE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PurM-like N-terminal" evidence="2">
        <location>
            <begin position="32"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="3">
        <location>
            <begin position="145"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="323" mass="33043" checksum="0334347E12FFCEFD" modified="2017-05-10" version="1">MPGKVHPDDLLSHVFGRTGEDDPAVVKGPADGEDAAAIDPPAGTLVVSSDPISLAASRVGTLGVHVACNDIAVSGADPRWLSVVLMLPSERALEPITRDIHAAASDLGATIVGGHSEYVDALERPIVSLTAMGMTEAFVPTGGAEPGDRVVLTGAAGIEGTAILAADFGGELDVPTDVVERGASFFEDVSVAADAQILREYATSMHDPTEGGVAAGLHEIARASDVRLDVEREAVPIREATRALCDAAGVDPLRIFGSGAVAGTVPESEVDDALAALSDADIPAAEVGVVSEGSPELRMDGESTVDPIEDDLYPLWAAADSEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXG8</accession>
    <name>A0A1U7EXG8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transport protein (Probable substrate cadmium)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">tp50</name>
        <name evidence="2" type="ordered locus">NP_3532A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323477.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXG8"/>
    <dbReference type="STRING" id="348780.NP_3532A"/>
    <dbReference type="EnsemblBacteria" id="CAI49857">
        <property type="protein sequence ID" value="CAI49857"/>
        <property type="gene ID" value="NP_3532A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703336"/>
    <dbReference type="KEGG" id="nph:NP_3532A"/>
    <dbReference type="eggNOG" id="arCOG11200">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1375555_0_0_2"/>
    <dbReference type="OrthoDB" id="351321at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004676">
        <property type="entry name" value="Cd-R_transporter"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03596">
        <property type="entry name" value="Cad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="192" mass="20161" checksum="4EC09B36725F9FB2" modified="2017-05-10" version="1">METVLLVGVWLFAVTHLDTLVVISAFCADNDYQLWEVLVGHYIGFCAGLVAAVAGAALAAELFQEWTFLLGSVPLGIGLWGLFHQPPETTVEKSRAVPNTLGRIGVVSVAGIGLSGENIAVFVPFFAGLSLAELLVVIGVYLVGAGIVFLAAYIIVSRVAADGIPGWLDRWLVPTVLVLVGGYVLATGLVLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXI7</accession>
    <name>A0A1U7EXI7_NATPD</name>
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        <submittedName>
            <fullName evidence="11">Kef-type transport system</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">kef4</name>
        <name evidence="11" type="synonym">tp41a</name>
        <name evidence="11" type="ordered locus">NP_3602A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323512.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXI7"/>
    <dbReference type="STRING" id="348780.NP_3602A"/>
    <dbReference type="EnsemblBacteria" id="CAI49892">
        <property type="protein sequence ID" value="CAI49892"/>
        <property type="gene ID" value="NP_3602A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703318"/>
    <dbReference type="KEGG" id="nph:NP_3602A"/>
    <dbReference type="eggNOG" id="arCOG01955">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005126_9_0_2"/>
    <dbReference type="OrthoDB" id="43518at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42751">
        <property type="entry name" value="SODIUM/HYDROGEN EXCHANGER FAMILY/TRKA DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42751:SF3">
        <property type="entry name" value="SODIUM_GLUTAMATE SYMPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="101"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="197"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="232"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="284"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="312"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="375"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="9">
        <location>
            <begin position="31"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="10">
        <location>
            <begin position="428"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="575"/>
            <end position="600"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI49892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="600" mass="65589" checksum="38ACC7BB83263F7D" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXM8</accession>
    <name>A0A1U7EXM8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3732A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323574.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXM8"/>
    <dbReference type="EnsemblBacteria" id="CAI49957">
        <property type="protein sequence ID" value="CAI49957"/>
        <property type="gene ID" value="NP_3732A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701851"/>
    <dbReference type="KEGG" id="nph:NP_3732A"/>
    <dbReference type="eggNOG" id="arCOG08958">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039020_0_0_2"/>
    <dbReference type="OrthoDB" id="304708at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="410" mass="46658" checksum="BD763FBF95F7CAC4" modified="2017-05-10" version="1">MPPERRDVLRLGTVGLGGLVAGCLDNGGADFGSGDATPTPEPEPDEEPTPETDPDEEPDEPTLDEPTRQLVAATDRVLDEIEWFATEYEDAMDAYVSALDRAIETVLQLENTTTITEGDIERLEDVAEHVEVTIQDELEPHFRLHGRVSRRNERAIERVERFGERGDRPAVNDELESLRSFYEGIRGFLFRQRNLSRDPIRNRLVEFLSRDDIGGDIDDILFEFRYRNGESLSTRAYTEDGQEDRDDPNLLGEPAATPAGSIRWSPTYTFTERFEPMSVPQGRRDELALVVNDWEDYDGTPVGVYTDRFHSWPVYIQRYHSPAAAETAASLLVDGPVFLEADWAVAIGDIEWRQARYRLDGDVIYAPFKQFGPYIITLAPSRTSIEDREGRDDDEPWTVPLDHTWLHIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXN3</accession>
    <name>A0A1U7EXN3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">tRNA (Cytidine/uridine-2'-O-)-methyltransferase</fullName>
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        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">NP_3772A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323594.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXN3"/>
    <dbReference type="STRING" id="348780.NP_3772A"/>
    <dbReference type="EnsemblBacteria" id="CAI49977">
        <property type="protein sequence ID" value="CAI49977"/>
        <property type="gene ID" value="NP_3772A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701861"/>
    <dbReference type="KEGG" id="nph:NP_3772A"/>
    <dbReference type="eggNOG" id="arCOG01018">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056931_0_1_2"/>
    <dbReference type="OrthoDB" id="372184at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002128">
        <property type="term" value="P:tRNA nucleoside ribose methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18093">
        <property type="entry name" value="SpoU-like_TrmJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004384">
        <property type="entry name" value="RNA_MeTrfase_TrmJ/LasT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00050">
        <property type="entry name" value="rRNA_methyl_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42786:SF2">
        <property type="entry name" value="TRNA (CYTIDINE_URIDINE-2'-O-)-METHYLTRANSFERASE TRMJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42786">
        <property type="entry name" value="TRNA/RRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004808">
        <property type="entry name" value="LasT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="tRNA/rRNA methyltransferase SpoU type" evidence="6">
        <location>
            <begin position="4"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="213"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="253"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="264" mass="29049" checksum="2BC673A975A33018" modified="2017-05-10" version="1">MPNIAVAVVGAETPGNVGTIARSMKNFGLSELYLVDPPELDPDGEAYGFAGHAREDVLPNATEVEFSYLTEQFYTVACTATTNEDATKHVRYPFIEPAALSDELAGLDADVCIVFGRERVGLTNDELAQLDRICSIPADGDYPVLNLAQAATIVLYELRELTVEETQHPKQAHERADEHEIEGLHETFSEYLHEVGHPEEKIPKTERLFRRLAGRASPTGREARTLRGVLRRGALVASGEIPRPGTSDPGDEPNQKDESNRENE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXN4</accession>
    <name>A0A1U7EXN4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Triosephosphate isomerase</fullName>
            <ecNumber evidence="2">5.3.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">tpiA2</name>
        <name evidence="2" type="ordered locus">NP_3716A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="5.3.1.1" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323566.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXN4"/>
    <dbReference type="STRING" id="348780.NP_3716A"/>
    <dbReference type="EnsemblBacteria" id="CAI49949">
        <property type="protein sequence ID" value="CAI49949"/>
        <property type="gene ID" value="NP_3716A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703358"/>
    <dbReference type="KEGG" id="nph:NP_3716A"/>
    <dbReference type="eggNOG" id="arCOG01087">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104921_0_0_2"/>
    <dbReference type="OrthoDB" id="9465at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004807">
        <property type="term" value="F:triose-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035990">
        <property type="entry name" value="TIM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000652">
        <property type="entry name" value="Triosephosphate_isomerase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003302">
        <property type="entry name" value="PRK04302.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00121">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51351">
        <property type="entry name" value="Triosephosphate isomerase (TIM)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51440">
        <property type="entry name" value="TIM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="230" mass="23526" checksum="755E9C5921072D9B" modified="2017-05-10" version="1">MSLTYPCFLVNYKTYEGTAGTDGLELATTIDAVRQQTGASFAVAPQTPDIYRIAEATDLPVVAQSVDSTAAGRGNGNISLASVAEAGASGALINHPESQDAFGDVGDIVAGCAEHGLESIVCVDSVELGRAAVAFDPDCLLFENPADIASDRALARTDPERVEAFVSMVQAERPDTRVLLGGGITTAADVAASLECGADAAGAASAFVDASDRSAWLSEIATALTEAEPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXT1</accession>
    <name>A0A1U7EXT1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3836A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323626.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXT1"/>
    <dbReference type="STRING" id="348780.NP_3836A"/>
    <dbReference type="EnsemblBacteria" id="CAI50009">
        <property type="protein sequence ID" value="CAI50009"/>
        <property type="gene ID" value="NP_3836A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701879"/>
    <dbReference type="KEGG" id="nph:NP_3836A"/>
    <dbReference type="eggNOG" id="arCOG04493">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123158_0_0_2"/>
    <dbReference type="OrthoDB" id="211802at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="190" mass="20774" checksum="6B6E42DB4C278906" modified="2017-05-10" version="1">MSGSQLRTAVADTSALVSLAVPHADSAFDTERTPDPLQYLLTSCDVFVPPEVLTELREITQYQDIHAAAASNVLAARTHYTVEGPYEYDATPDSRPTFGLDDGETDGIVLANALSADGFLTDEFGGTNFPLIHAVLQGPRIVPTPRLICDYARNGHMTHEEAKALITMISPHRSWENSPYVGQLLQYLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXX7</accession>
    <name>A0A1U7EXX7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Halocyanin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">hcp1</name>
        <name evidence="12" type="ordered locus">NP_3954A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="9">Binds 1 copper ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Periplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323684.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXX7"/>
    <dbReference type="STRING" id="348780.NP_3954A"/>
    <dbReference type="EnsemblBacteria" id="CAI50068">
        <property type="protein sequence ID" value="CAI50068"/>
        <property type="gene ID" value="NP_3954A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702800"/>
    <dbReference type="KEGG" id="nph:NP_3954A"/>
    <dbReference type="eggNOG" id="arCOG02921">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084115_1_2_2"/>
    <dbReference type="OrthoDB" id="11088at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04220">
        <property type="entry name" value="Halocyanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002386">
        <property type="entry name" value="Amicyanin/Pseudoazurin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000923">
        <property type="entry name" value="BlueCu_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028871">
        <property type="entry name" value="BlueCu_1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017533">
        <property type="entry name" value="Halocyanin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03102">
        <property type="entry name" value="halo_cynanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34192">
        <property type="entry name" value="PLASTOCYANIN MAJOR ISOFORM, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34192:SF10">
        <property type="entry name" value="PLASTOCYANIN MAJOR ISOFORM, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00127">
        <property type="entry name" value="Copper-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00155">
        <property type="entry name" value="AMICYANIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00196">
        <property type="entry name" value="COPPER_BLUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 9" id="KW-0186">Copper</keyword>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0574">Periplasm</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Blue (type 1) copper" evidence="11">
        <location>
            <begin position="77"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="26"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="26"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR602386-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI50068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="165" mass="17227" checksum="A8052F2FF40ECC44" modified="2017-05-10" version="1">MKDISRRRFVLGTGATVAAATLAGCNGNGNGNGNGNGNGNGEPDTPEGRADQFLTDNDALMYDGDITDETGQDEVVVATGAGNNGFAFDPAAVRVDAGTTVVFEWTGEGGAHNVVDEPGSDFEFESDRVDEEGHTFEVTFDEEGVALYVCTPHRAQGMYGAVIVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY17</accession>
    <name>A0A1U7EY17_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">AAA-type ATPase (CDC48 subfamily)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cdc48b</name>
        <name evidence="9" type="ordered locus">NP_3998A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family. CDC48 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323706.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY17"/>
    <dbReference type="STRING" id="348780.NP_3998A"/>
    <dbReference type="EnsemblBacteria" id="CAI50090">
        <property type="protein sequence ID" value="CAI50090"/>
        <property type="gene ID" value="NP_3998A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701934"/>
    <dbReference type="KEGG" id="nph:NP_3998A"/>
    <dbReference type="eggNOG" id="arCOG01308">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_12_2_2"/>
    <dbReference type="OrthoDB" id="77269at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19511">
        <property type="entry name" value="RecA-like_CDC48_r2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000007">
        <property type="entry name" value="AAA family ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000057">
        <property type="entry name" value="AAA family ATPase, CDC48 subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000189">
        <property type="entry name" value="AAA family ATPase, CDC48 subfamily"/>
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    </dbReference>
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        <property type="entry name" value="Cell division control 48"/>
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    </dbReference>
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        <property type="entry name" value="Cell division cycle protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000012">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005938">
        <property type="entry name" value="AAA_ATPase_CDC48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004201">
        <property type="entry name" value="Cdc48_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01243">
        <property type="entry name" value="CDC48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF171">
        <property type="entry name" value="NUCLEAR VALOSIN-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02933">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02359">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01072">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="Cdc48 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CDC48 N-terminal subdomain" evidence="8">
        <location>
            <begin position="2"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="CDC48" evidence="7">
        <location>
            <begin position="102"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="229"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="502"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="162"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="719"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="748"/>
            <end position="759"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI50090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="759" mass="82440" checksum="FF953C2B068EC93C" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYB0</accession>
    <name>A0A1U7EYB0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC-type transport system periplasmic substrate-binding protein (Probable substrate branched-chain amino acids)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">livJ6</name>
        <name evidence="3" type="ordered locus">NP_4238A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323826.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYB0"/>
    <dbReference type="STRING" id="348780.NP_4238A"/>
    <dbReference type="EnsemblBacteria" id="CAI50210">
        <property type="protein sequence ID" value="CAI50210"/>
        <property type="gene ID" value="NP_4238A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702010"/>
    <dbReference type="KEGG" id="nph:NP_4238A"/>
    <dbReference type="eggNOG" id="arCOG01024">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027128_1_4_2"/>
    <dbReference type="OrthoDB" id="264684at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051010">
        <property type="entry name" value="BCAA_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028081">
        <property type="entry name" value="Leu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019546">
        <property type="entry name" value="TAT_signal_bac_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01409">
        <property type="entry name" value="TAT_signal_seq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30483:SF37">
        <property type="entry name" value="ABC TRANSPORTER SUBSTRATE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30483">
        <property type="entry name" value="LEUCINE-SPECIFIC-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13458">
        <property type="entry name" value="Peripla_BP_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Leucine-binding protein" evidence="2">
        <location>
            <begin position="50"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="447" mass="48665" checksum="793F4B63A2A7EB47" modified="2017-05-10" version="1">MSDNTTHASRRRFLKGAAAAGGIGAFAGCALLDDAGGEEQVEGIPDEPFRIVHFTFESGPPAFYGEPMINVVDMMVDRINDRGGLLGEREIEVVDRIDEDVGVESMRGSVRQIAQNDEADMLMGLVSSAHSLSIAPAAEENQIPFIVTAPGTYQLFEENPDLDYVVRPAGSNAAGAVGGARLVEQMDDVETVVTIDPDYAWGHDHRDMFTAAIERIRPDIEVLETRTPEPFISDYSAHVNAIQDLEPDLLASSLWGGDISTFTSQAVDGGLFDDIGTALLYGDTGAPLLDSLGDDMPENVIMGGRGGYQPNYRYEISSDHRAFVDDYFDRYDEMPGWGAYHEWASFKALEAGVQQAVEVTGNWPTGSQLMNSIRGLSFEMSHGSHVMAQAGGRQASAPAVFGQPTPDHDHPVDHHLLTDYEFIPAHECNPPDGVMTFDWVEQIDPVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYB3</accession>
    <name>A0A1U7EYB3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4220A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323817.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYB3"/>
    <dbReference type="STRING" id="348780.NP_4220A"/>
    <dbReference type="EnsemblBacteria" id="CAI50201">
        <property type="protein sequence ID" value="CAI50201"/>
        <property type="gene ID" value="NP_4220A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702008"/>
    <dbReference type="KEGG" id="nph:NP_4220A"/>
    <dbReference type="eggNOG" id="arCOG06248">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2243758_0_0_2"/>
    <dbReference type="OrthoDB" id="297731at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058893">
        <property type="entry name" value="RHH-containing"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26048">
        <property type="entry name" value="RHH_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="109" mass="12390" checksum="DEDDF5A417DC3A76" modified="2017-05-10" version="1">MGLEDLSDDIETAYSDLGEELSVDLDTETRNELAMLQSAYEPDDPDELVRRAVHMLFQTAIETGNLDFHLRSGYDVTYDEYLSGMTFEEMGGGSDLFGQNDDNARRYQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYB4</accession>
    <name>A0A1U7EYB4_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Adenine DNA glycosylase</fullName>
            <ecNumber evidence="4">3.2.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">mutY</name>
        <name evidence="14" type="ordered locus">NP_4222A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolyzes free adenine bases from 7,8-dihydro-8-oxoguanine:adenine mismatched double-stranded DNA, leaving an apurinic site.</text>
            <dbReference type="EC" id="3.2.2.31"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Nth/MutY family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.31" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323818.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYB4"/>
    <dbReference type="STRING" id="348780.NP_4222A"/>
    <dbReference type="EnsemblBacteria" id="CAI50202">
        <property type="protein sequence ID" value="CAI50202"/>
        <property type="gene ID" value="NP_4222A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702954"/>
    <dbReference type="KEGG" id="nph:NP_4222A"/>
    <dbReference type="eggNOG" id="arCOG00462">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012862_2_0_2"/>
    <dbReference type="OrthoDB" id="206280at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034039">
        <property type="term" value="F:8-oxo-7,8-dihydroguanine DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035485">
        <property type="term" value="F:adenine/guanine mispair binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032357">
        <property type="term" value="F:oxidized purine DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000701">
        <property type="term" value="F:purine-specific mismatch base pair DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000445">
        <property type="entry name" value="HhH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044298">
        <property type="entry name" value="MIG/MutY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42944">
        <property type="entry name" value="ADENINE DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42944:SF1">
        <property type="entry name" value="ADENINE DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00633">
        <property type="entry name" value="HHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="12 14" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="8 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="13">
        <location>
            <begin position="47"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAI50202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="307" mass="34833" checksum="23F58502EB09747D" modified="2017-05-10" version="1">MEDGPDTLPADVDAVRAALIEWYETDHRSYPWRETEDPYEILVSEVMSQQTQLDRVVEAWHAFLDEWPTAEALAEADRAAVVGFWTDHSLGYNNRAKYLHEAARQVRDEHDGEFPRTPDGLQELMGVGPYTANAVASFAFNNGDAVVDTNVKRVLYRAFSELHDKEEPPYQHIADELLPKGRSRVWNNAIMELGAVACGKTPRCDEAGCPWREWCDAYDTGDFTAPDVPTQPSFEGSRRQFRGRVVRALNEYGELPIDELGPRIRVDYSPDGEYGRAWLRSLLEDLSDEGMVQLTERDGAVIARLQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYD4</accession>
    <name>A0A1U7EYD4_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">enoyl-CoA hydratase</fullName>
            <ecNumber evidence="5">4.2.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">hbd3</name>
        <name evidence="15" type="synonym">fadA3</name>
        <name evidence="15" type="ordered locus">NP_4322A</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.17" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323868.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYD4"/>
    <dbReference type="STRING" id="348780.NP_4322A"/>
    <dbReference type="EnsemblBacteria" id="CAI50252">
        <property type="protein sequence ID" value="CAI50252"/>
        <property type="gene ID" value="NP_4322A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702794"/>
    <dbReference type="KEGG" id="nph:NP_4322A"/>
    <dbReference type="eggNOG" id="arCOG00249">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010448_2_1_2"/>
    <dbReference type="OrthoDB" id="39812at2157"/>
    <dbReference type="UniPathway" id="UPA00659"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016509">
        <property type="term" value="F:long-chain (3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006072">
        <property type="term" value="P:glycerol-3-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000009">
        <property type="entry name" value="Carnitinyl-CoA dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000009">
        <property type="entry name" value="Fatty oxidation complex, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006176">
        <property type="entry name" value="3-OHacyl-CoA_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006108">
        <property type="entry name" value="3HC_DH_C"/>
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        <property type="entry name" value="G3P_DH_NAD-dep"/>
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        <property type="entry name" value="ECH_1"/>
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        <property type="entry name" value="GPDHDRGNASE"/>
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        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="7 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="8"/>
            <end position="188"/>
        </location>
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    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase C-terminal" evidence="13">
        <location>
            <begin position="191"/>
            <end position="286"/>
        </location>
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    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase C-terminal" evidence="13">
        <location>
            <begin position="303"/>
            <end position="386"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00008750"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAI50252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="655" mass="71235" checksum="85FEC5B2EABB75CB" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYF4</accession>
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        <submittedName>
            <fullName evidence="14">Propionyl-CoA carboxylase biotin carboxylase component</fullName>
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        <name evidence="14" type="primary">pccA2</name>
        <name evidence="14" type="ordered locus">NP_4368A</name>
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        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
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            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
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    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
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                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
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            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Co(2+)</name>
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        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>biotin</name>
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        </cofactor>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
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    <dbReference type="KEGG" id="nph:NP_4368A"/>
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        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="entry name" value="Propionyl-CoA carboxylase subunit alpha"/>
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        <property type="entry name" value="ATP-grasp"/>
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        <property type="entry name" value="BC-like_N"/>
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        <property type="entry name" value="Biotin_BS"/>
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        <property type="entry name" value="Biotin_carboxylation_dom"/>
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        <property type="entry name" value="Biotin_COase_C"/>
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        <property type="entry name" value="Biotin_lipoyl"/>
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        <property type="entry name" value="CPAse_ATP-bd"/>
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    </dbReference>
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        <property type="entry name" value="CARBOXYLASE:PYRUVATE/ACETYL-COA/PROPIONYL-COA CARBOXYLASE"/>
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    </dbReference>
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        <property type="entry name" value="METHYLCROTONOYL-COA CARBOXYLASE SUBUNIT ALPHA, MITOCHONDRIAL-RELATED"/>
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        <property type="entry name" value="Biotin_carb_C"/>
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        <property type="entry name" value="Biotin_lipoyl"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CPSASE_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="8" id="KW-0092">Biotin</keyword>
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    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="459"/>
        </location>
    </feature>
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        <location>
            <begin position="121"/>
            <end position="329"/>
        </location>
    </feature>
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        <location>
            <begin position="536"/>
            <end position="613"/>
        </location>
    </feature>
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        <location>
            <begin position="512"/>
            <end position="541"/>
        </location>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAI50275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="613" mass="66128" checksum="0CB572AD37B4C19F" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYI4</accession>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ordered locus">NP_4418A</name>
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        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        </lineage>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAI50300.1"/>
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    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_1381418_0_0_2"/>
    <dbReference type="OrthoDB" id="108920at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055834">
        <property type="entry name" value="DUF7411"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011155">
        <property type="entry name" value="PRK14561.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24167">
        <property type="entry name" value="DUF7411"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="197" mass="21655" checksum="456697DC03D3E7B2" modified="2017-05-10" version="1">MACGLLFSAGKDSSLAALLLERFYDVTLVTAHFGTTDDWRHARDAAAELGFEFETLELDAAVAEEAVERMRNEGFPRPGIQQVHEAALEAAAAEYDVVADGTRRDDRVPTVSRAFAQSLEDRHGIEYVAPLSGFGRGAVDDLVETYLEVEQGPSETVPKGDYEAELRALIRERWGASAIDDIFPDHEQTRVRGRADG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYP0</accession>
    <name>A0A1U7EYP0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Tritrans,polycis-undecaprenyl-diphosphate synthase (Geranylgeranyl-diphosphate specific)</fullName>
            <ecNumber evidence="2">2.5.1.89</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">uppS2</name>
        <name evidence="2" type="ordered locus">NP_4544A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.5.1.89" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323978.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYP0"/>
    <dbReference type="STRING" id="348780.NP_4544A"/>
    <dbReference type="EnsemblBacteria" id="CAI50363">
        <property type="protein sequence ID" value="CAI50363"/>
        <property type="gene ID" value="NP_4544A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703447"/>
    <dbReference type="KEGG" id="nph:NP_4544A"/>
    <dbReference type="eggNOG" id="arCOG01532">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038505_2_1_2"/>
    <dbReference type="OrthoDB" id="140576at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045547">
        <property type="term" value="F:ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016094">
        <property type="term" value="P:polyprenol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1180.10:FF:000016">
        <property type="entry name" value="Undecaprenyl diphosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="entry name" value="Decaprenyl diphosphate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001441">
        <property type="entry name" value="UPP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291:SF28">
        <property type="entry name" value="UNDECAPRENYL DIPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01255">
        <property type="entry name" value="Prenyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="Undecaprenyl diphosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="202" mass="22920" checksum="97B61B8D2A9F19F3" modified="2017-05-10" version="1">MGLYDRYLAARVRLSNEDAPNHIALVITERDLLEPNAYGTLTSFLRWAFDTGTETVTIYVSVLDAEAIPTLRQTLSELESPREVAIRGPEADAPADAPVQVSIGLGGREEFAAAVRQIAEDAAAGALAPDDIDESEIERRLVFRNQPDLVIKTGAERLSDFLIWQSVYSELYFTDVNWRAFRRRDFLRAVREFQERQRRFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYT0</accession>
    <name>A0A1U7EYT0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">glycine dehydrogenase (aminomethyl-transferring)</fullName>
            <ecNumber evidence="1">1.4.4.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">gcvP2</name>
        <name evidence="8" type="ordered locus">NP_4594A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[glycine-cleavage complex H protein] + glycine + H(+) = N(6)-[(R)-S(8)-aminomethyldihydrolipoyl]-L-lysyl-[glycine-cleavage complex H protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:24304"/>
            <dbReference type="Rhea" id="RHEA-COMP:10494"/>
            <dbReference type="Rhea" id="RHEA-COMP:10495"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="1.4.4.2"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="1.4.4.2" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324003.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYT0"/>
    <dbReference type="STRING" id="348780.NP_4594A"/>
    <dbReference type="EnsemblBacteria" id="CAI50388">
        <property type="protein sequence ID" value="CAI50388"/>
        <property type="gene ID" value="NP_4594A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702745"/>
    <dbReference type="KEGG" id="nph:NP_4594A"/>
    <dbReference type="eggNOG" id="arCOG00076">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004620_5_0_2"/>
    <dbReference type="OrthoDB" id="371967at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016594">
        <property type="term" value="F:glycine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004375">
        <property type="term" value="F:glycine dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000224">
        <property type="entry name" value="Probable glycine dehydrogenase (decarboxylating) subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049316">
        <property type="entry name" value="GDC-P_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020581">
        <property type="entry name" value="GDC_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003346">
        <property type="entry name" value="PRK04366.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11773:SF1">
        <property type="entry name" value="GLYCINE DEHYDROGENASE (DECARBOXYLATING), MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11773">
        <property type="entry name" value="GLYCINE DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21478">
        <property type="entry name" value="GcvP2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="6">
        <location>
            <begin position="150"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="Glycine dehydrogenase C-terminal" evidence="7">
        <location>
            <begin position="351"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="442"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF21478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI50388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="477" mass="51017" checksum="706CD194537CCE08" modified="2017-05-10" version="1">MSDDDPLGYDQARWMADEETYEPLLAEKSTESVTVDSTLPDELTRDELELPALSEPELARHYTRLSEMNYGVDSGPFPLGSCTMKYNPKFTEDVAADPKAAVHPARSEGTVQGVLALQHGLQEQLATIGGMDAVTLQPPAGAAGEFAGILMAKAYHEANGDDRTEVIVPDSAHGTNFASAAMAGYDVVTLPSADDGCVDLDALSAALSEDTAALMLTNPNTLGLFERDIESIAELVHDVGGLLYYDGANLNALLGRARPGDMGFDIMHYNVHKTFATPHGGGGPGAGPVGVTEELADFLPAPHVRETDSGYERYEPDHTIGKVHGYQGNWLVLLKAYAYIARLGDEGLTDASAKAVLNANYLATQVDYEVPFGPFHHEFVASAGDQDAADVAKRMLDYGVHPPTTKWPDIVDEALMTEPTEIESKRTLDQLAAAFNAARADDADTLAAAPERTAAGRIDQTSAARDPRLSWQALDDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYV5</accession>
    <name>A0A1U7EYV5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribonuclease VapC</fullName>
            <shortName evidence="8">RNase VapC</shortName>
            <ecNumber evidence="8">3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Putative toxin VapC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">vapC</name>
        <name evidence="10" type="ordered locus">NP_4666A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Toxic component of a toxin-antitoxin (TA) system. An RNase.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the PINc/VapC protein family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="8"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324038.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYV5"/>
    <dbReference type="EnsemblBacteria" id="CAI50424">
        <property type="protein sequence ID" value="CAI50424"/>
        <property type="gene ID" value="NP_4666A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702136"/>
    <dbReference type="KEGG" id="nph:NP_4666A"/>
    <dbReference type="eggNOG" id="arCOG02219">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118482_3_4_2"/>
    <dbReference type="OrthoDB" id="147588at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:RNA nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090729">
        <property type="term" value="F:toxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00265">
        <property type="entry name" value="VapC_Nob1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050556">
        <property type="entry name" value="Type_II_TA_system_RNase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022907">
        <property type="entry name" value="VapC_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33653">
        <property type="entry name" value="RIBONUCLEASE VAPC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33653:SF1">
        <property type="entry name" value="RIBONUCLEASE VAPC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0800">Toxin</keyword>
    <keyword evidence="2 8" id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="domain" description="PIN" evidence="9">
        <location>
            <begin position="5"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI50424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="136" mass="14893" checksum="4E72FA3FBC9EAF03" modified="2017-05-10" version="1">MTLLVIDTNLLSDYLNGTDEARAFLAAFERDRWGVPSIVLFEALMGSLYGHIDATPDEIVRSVDSSMEVLETGMETAIEAQELQAALLSRGAPVDQLDALIAAAALEHGGTFATAEKQFWTDDVQEIIQVEPYDPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYW5</accession>
    <name>A0A1U7EYW5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4686A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324048.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYW5"/>
    <dbReference type="EnsemblBacteria" id="CAI50434">
        <property type="protein sequence ID" value="CAI50434"/>
        <property type="gene ID" value="NP_4686A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702142"/>
    <dbReference type="KEGG" id="nph:NP_4686A"/>
    <dbReference type="eggNOG" id="arCOG04497">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113180_0_0_2"/>
    <dbReference type="OrthoDB" id="198445at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058703">
        <property type="entry name" value="PIN-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26425">
        <property type="entry name" value="PIN_halo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="178" mass="19396" checksum="DA888F5EB7BAD941" modified="2017-05-10" version="1">MDESSPLSPEHVTLVDTNVFISAGNPGTSKHRRFRQVVRRASVTLFVPSRVEEEIEQGGVRPALERAQDEGWAKTVDAPTVSHSKATTAQDIARRAIASKSTDKDEHDVEKADPVFAGLAVEYLLDKKTADTVTVITADRIACEAIETAISSLGYTGQVSIITLWDLIDAEDDDFTLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYZ8</accession>
    <name>A0A1U7EYZ8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cysteine synthase</fullName>
            <ecNumber evidence="8">2.5.1.47</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cysK1</name>
        <name evidence="8" type="ordered locus">NP_4748A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.47" evidence="8"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324078.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYZ8"/>
    <dbReference type="STRING" id="348780.NP_4748A"/>
    <dbReference type="EnsemblBacteria" id="CAI50465">
        <property type="protein sequence ID" value="CAI50465"/>
        <property type="gene ID" value="NP_4748A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702653"/>
    <dbReference type="KEGG" id="nph:NP_4748A"/>
    <dbReference type="eggNOG" id="arCOG01430">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021018_1_0_2"/>
    <dbReference type="OrthoDB" id="10138at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004124">
        <property type="term" value="F:cysteine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01561">
        <property type="entry name" value="CBS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000016">
        <property type="entry name" value="Cysteine synthase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="7">
        <location>
            <begin position="14"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI50465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="329" mass="34711" checksum="2E6A586C7B2E7F88" modified="2017-05-10" version="1">MTTHREPLASVVETVGETPLVRVQASPPDVPVYAKLESFNPGASIKDRVGVHIIEGLLESGDLDPGGTVIEPTAGNTGIGMAMAATQYDVDAVFTVPEKFSVEKQVLMRALGADIVNTPTEEGMGGAIERAHELAADEPNAVVPQQFSTDLNVQAHAETTAPEVYEALDGEVGAIVAGCGTAGTLMGMARYALDTNPATHIVAVEPEGSVFRRAAGDDAEASEYNIEGIGTHDHTTNELFDPELVDEIQSVSDRDAQKELKRLAAEEGQLVATSSGAASVVALDVARRAADGDLSLPHDTVVTVFPDSSERYLSKGIYDSYEEWIHGAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ46</accession>
    <name>A0A1U7EZ46_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Large ribosomal subunit protein eL32</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 8" type="primary">rpl32e</name>
        <name evidence="8" type="ordered locus">NP_4888A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eL32 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324147.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ46"/>
    <dbReference type="STRING" id="348780.NP_4888A"/>
    <dbReference type="EnsemblBacteria" id="CAI50535">
        <property type="protein sequence ID" value="CAI50535"/>
        <property type="gene ID" value="NP_4888A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703140"/>
    <dbReference type="KEGG" id="nph:NP_4888A"/>
    <dbReference type="eggNOG" id="arCOG00781">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071479_0_0_2"/>
    <dbReference type="OrthoDB" id="372100at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00513">
        <property type="entry name" value="Ribosomal_L32_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00810">
        <property type="entry name" value="Ribosomal_eL32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010995">
        <property type="entry name" value="DNA_repair_Rad51/TF_NusA_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001515">
        <property type="entry name" value="Ribosomal_eL32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023654">
        <property type="entry name" value="Ribosomal_eL32_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018263">
        <property type="entry name" value="Ribosomal_eL32_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036351">
        <property type="entry name" value="Ribosomal_eL32_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006332">
        <property type="entry name" value="PRK08562.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009401">
        <property type="entry name" value="PRK12766.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L32 AND DNA-DIRECTED RNA POLYMERASE II, SUBUNIT N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN L32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14520">
        <property type="entry name" value="HHH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01655">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01393">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47794">
        <property type="entry name" value="Rad51 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52042">
        <property type="entry name" value="Ribosomal protein L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00580">
        <property type="entry name" value="RIBOSOMAL_L32E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 8" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Helix-hairpin-helix DNA-binding motif class 1" evidence="7">
        <location>
            <begin position="10"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="Helix-hairpin-helix DNA-binding motif class 1" evidence="7">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="60"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="64"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="144"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI50535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="240" mass="26781" checksum="67014C769E25D36E" modified="2017-05-10" version="1">MSDDAADEYEELTDISGVGEAKAETLREAGIETVDDLREKSQDELADIDGVGNALAARIKADVGDLEVDAETDTEVEDETPEEADDEAEAEDVETELQPRGLADKTPELSDEEERLLTERKRVGKPQFNRQDYHKDKRTPTSWRRPRGTLSKQRRGIKGKGPTVEAGFRTPKEVRGKHPSGFEEVYVENVDDLEGVDGDREAARIGSTVGARKRERIEEVAEEEGIRVLNPTYVEVEVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ96</accession>
    <name>A0A1U7EZ96_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_5018A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324210.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ96"/>
    <dbReference type="STRING" id="348780.NP_5018A"/>
    <dbReference type="EnsemblBacteria" id="CAI50600">
        <property type="protein sequence ID" value="CAI50600"/>
        <property type="gene ID" value="NP_5018A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702229"/>
    <dbReference type="KEGG" id="nph:NP_5018A"/>
    <dbReference type="eggNOG" id="arCOG07571">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_716896_0_0_2"/>
    <dbReference type="OrthoDB" id="167544at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="368" mass="39993" checksum="930A41F8DFE7B57E" modified="2017-05-10" version="1">MKLHRRTALRLAGVGVGTAVAGCLDPETDNGETNGITTETMPLAVRTGRPAWDRDDAVGNVVVIDDDDRQQAVFGRYDDAVGEDRSDEFQSFLENIDYGDERLVVVESVGPNACHDRLEIADSRLDDGELVARADVIDTSEEETACAEVVTYPSALLRVTFEDDPADTAAVAVTNGWGETATVVGTADDPLGPTVADLTGHVRPDDDPEPVPPLDCDAEEVERHPQGVDEAEIEWGTVEADSDPALALRVDEPAYEYGETATIRLTNVADRFVDTGNRNKYNLQVSTDDGWQDVRVADADRPMGYTDEEIVHSPGDGFEWQVELTEEAIEEKSIHDLAVCPELPAGRYRFVFWGVIGGGVAVAFDLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZD7</accession>
    <name>A0A1U7EZD7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ADP-ribose pyrophosphatase</fullName>
            <ecNumber evidence="2">3.6.1.13</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5148A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.1.13" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324275.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZD7"/>
    <dbReference type="STRING" id="348780.NP_5148A"/>
    <dbReference type="EnsemblBacteria" id="CAI50665">
        <property type="protein sequence ID" value="CAI50665"/>
        <property type="gene ID" value="NP_5148A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702607"/>
    <dbReference type="KEGG" id="nph:NP_5148A"/>
    <dbReference type="eggNOG" id="arCOG00215">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030805_0_2_2"/>
    <dbReference type="OrthoDB" id="372037at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047631">
        <property type="term" value="F:ADP-ribose diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00885">
        <property type="entry name" value="cinA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050101">
        <property type="entry name" value="CinA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056596">
        <property type="entry name" value="FLAD1_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13939">
        <property type="entry name" value="NICOTINAMIDE-NUCLEOTIDE AMIDOHYDROLASE PNCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13939:SF0">
        <property type="entry name" value="NMN AMIDOHYDROLASE-LIKE PROTEIN YFAY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24102">
        <property type="entry name" value="FLAD1_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoaB/Mog" evidence="1">
        <location>
            <begin position="4"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="242" mass="25897" checksum="3162D1DE65B67563" modified="2017-05-10" version="1">MDAALVTVGDELLAGDTENTNATWLAGRLSDRGVAVKRTLVVPDVRAVIADAVGRYSDAFDAVLVTGGLGRTPDDVTVEAVADAFDRGLVVDEVALADVERTLEAIAGEYPDLEVDAAAEARLPRGSRPLINHAGLSPGCVVDNVYVLPGIPSEMERMFELVAEEFNGDTESRYLYTEEPEANLLDRLEDVRDRFGVAVGCYPDREAGHNRLQLKATDPDALDDAASWLADTLALVDPDDAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZE3</accession>
    <name>A0A1U7EZE3_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_5150A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324276.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZE3"/>
    <dbReference type="STRING" id="348780.NP_5150A"/>
    <dbReference type="EnsemblBacteria" id="CAI50666">
        <property type="protein sequence ID" value="CAI50666"/>
        <property type="gene ID" value="NP_5150A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702273"/>
    <dbReference type="KEGG" id="nph:NP_5150A"/>
    <dbReference type="eggNOG" id="arCOG03143">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117036_0_0_2"/>
    <dbReference type="OrthoDB" id="115795at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043852">
        <property type="entry name" value="DUF5814"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19131">
        <property type="entry name" value="DUF5814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="151" mass="16735" checksum="60F003A3D4B65B3F" modified="2017-05-10" version="1">MAITDKIYVKNHRQLASQLDTSFPKSAFSGATLDVLFTGDGIAKLDDASRDRVLDFAEDFLDCDCQGHPHCGCPERKFIAYLLELRAQGLGPDAIVDVMGDDYMLYAYPGDVLSFLDNSVRTLEAAERLADVDGQNEAGEEIRRKRRELSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZF2</accession>
    <name>A0A1U7EZF2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5176A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324289.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZF2"/>
    <dbReference type="STRING" id="348780.NP_5176A"/>
    <dbReference type="EnsemblBacteria" id="CAI50679">
        <property type="protein sequence ID" value="CAI50679"/>
        <property type="gene ID" value="NP_5176A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702282"/>
    <dbReference type="KEGG" id="nph:NP_5176A"/>
    <dbReference type="eggNOG" id="arCOG06212">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_163803_0_0_2"/>
    <dbReference type="OrthoDB" id="195084at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043899">
        <property type="entry name" value="DUF5789"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19102">
        <property type="entry name" value="DUF5789"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="94" mass="10167" checksum="5FF2CB5726BB4E45" modified="2017-05-10" version="1">MSDDDEEGPAVPLGEGEPVEGAPLARVASRLHYAIEKSEVVRREGETTIRTPDGPRELADVLEETDAVYFDTRQAFESAVRDVIGTGPVPTAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZH2</accession>
    <name>A0A1U7EZH2_NATPD</name>
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        <submittedName>
            <fullName evidence="8">Major facilitator superfamily transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">tp19</name>
        <name evidence="8" type="ordered locus">NP_5220A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324311.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZH2"/>
    <dbReference type="STRING" id="348780.NP_5220A"/>
    <dbReference type="EnsemblBacteria" id="CAI50701">
        <property type="protein sequence ID" value="CAI50701"/>
        <property type="gene ID" value="NP_5220A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703295"/>
    <dbReference type="KEGG" id="nph:NP_5220A"/>
    <dbReference type="eggNOG" id="arCOG00134">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_5_14_2"/>
    <dbReference type="OrthoDB" id="200998at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050189">
        <property type="entry name" value="MFS_Efflux_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43124:SF3">
        <property type="entry name" value="CHLORAMPHENICOL EFFLUX PUMP RV0191"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43124">
        <property type="entry name" value="PURINE EFFLUX PUMP PBUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="126"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="271"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="295"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="330"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="359"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="6"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI50701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="396" mass="40577" checksum="24A09EACDAF89561" modified="2017-05-10" version="1">MSRRQLFGSLCAMVFLVNLARVVFAPLVQPVAADFAVTPAALGVVATAAWLGSAAPRLPTGYLLTRVPRHTVVAATGLLLVGTSAGTAFAPSLPWLTVGAFLMGISSGAYFIAANPLVTELFPSRVGWALGVHGMASQIAAVGAPLAVSAVLLVSGWRATFLLLSALAFVTTVVLVVAARRTELPDAGAKDRSLLAAARAQWPIILTGIVIVGSSGFLWNGLFNLYGPYLAAEKGIDEATGRTLLSVLFAAGVPAFLLAGELADRVRNVPLLLAIIIGFAASVFALTFAESLLAVVAVSIVVGVVIHALFPVIDTYMLASIPDENRASAYALFSSGMMTAQAFGSGGVGWLVASGVSYTAAYRGLAAIVGATGVGLFVLHRFGRLPTGGTPGETPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILT4</accession>
    <name>Q3ILT4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3316A</name>
        <name evidence="3" type="ordered locus">NP_7040A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>PL23</name>
    </geneLocation>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
            <strain evidence="3">Gabara</strain>
            <plasmid evidence="3">PL23</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CR936259">
        <property type="protein sequence ID" value="CAI50936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323370.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILT4"/>
    <dbReference type="STRING" id="348780.NP_3316A"/>
    <dbReference type="EnsemblBacteria" id="CAI49749">
        <property type="protein sequence ID" value="CAI49749"/>
        <property type="gene ID" value="NP_3316A"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI50936">
        <property type="protein sequence ID" value="CAI50936"/>
        <property type="gene ID" value="NP_7040A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703480"/>
    <dbReference type="KEGG" id="nph:NP_3316A"/>
    <dbReference type="KEGG" id="nph:NP_7040A"/>
    <dbReference type="HOGENOM" id="CLU_2115549_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="114" mass="11540" checksum="F1DCF873DFFE3C53" modified="2005-11-08" version="1">MTTPDSPELEDSSLACFGQGTEGDLVECVTTDLSAAAGGTTMMGLLIGGTLLLSLYIAGDGDPAVPTVVTILVGGTLVPMLPPHLTPFAYSIVVLGIAFGGMAAAQRWLLRGGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILU5</accession>
    <name>Q3ILU5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3294A</name>
        <name evidence="3" type="ordered locus">NP_7018A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>PL23</name>
    </geneLocation>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
            <strain evidence="3">Gabara</strain>
            <plasmid evidence="3">PL23</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CR936259">
        <property type="protein sequence ID" value="CAI50925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323359.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILU5"/>
    <dbReference type="STRING" id="348780.NP_3294A"/>
    <dbReference type="EnsemblBacteria" id="CAI49738">
        <property type="protein sequence ID" value="CAI49738"/>
        <property type="gene ID" value="NP_3294A"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI50925">
        <property type="protein sequence ID" value="CAI50925"/>
        <property type="gene ID" value="NP_7018A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703469"/>
    <dbReference type="KEGG" id="nph:NP_3294A"/>
    <dbReference type="KEGG" id="nph:NP_7018A"/>
    <dbReference type="HOGENOM" id="CLU_2802378_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="67" mass="6950" checksum="234CCEB32F950F9B" modified="2005-11-08" version="1">MRGSVWTVVVLAVGLAMLPLILDFGDAEGSLTADLFGIIAVFDGILGLLVLVAAMAAFVAYFQSDGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILV5</accession>
    <name>Q3ILV5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF91 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_6270A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324512.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILV5"/>
    <dbReference type="EnsemblBacteria" id="CAI50915">
        <property type="protein sequence ID" value="CAI50915"/>
        <property type="gene ID" value="NP_6270A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694662"/>
    <dbReference type="KEGG" id="nph:NP_6270A"/>
    <dbReference type="HOGENOM" id="CLU_800789_0_0_2"/>
    <dbReference type="OrthoDB" id="191938at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="347" mass="39676" checksum="D678768CA6DB6CB5" modified="2005-11-08" version="1">MLRVGDEEDVSTLETVSLKDEGFREDDLREWIINSPESILGEDFRLVGREVSVKQIGDGIDLLAIDRDANVVAIELKRGALKPKVDFQGLKYAAYTSHWDYSKLRDQFEKFKSTSWGRTLYEEETTFTEVLDEFCNEDYTLNQDQRIVFVGESVRERLDIVLRWLSDRSIDISVIEFQLLKDDNRLYLDAEQTIPTPEHTMTDVSPDTSDEPWKEDGQSWHLNERSNEETAGLLEDVTAVLQDLEFLNGPEWGQKHYVAFNKGRKRRIAIRTKRTLFHIDLYDVDTASLDAAEIADALGIDVESVAVKKDMRGSGRSGVRITCVPNQAIDFDAIESQAREILGETTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILW4</accession>
    <name>Q3ILW4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_6252A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILW4"/>
    <dbReference type="EnsemblBacteria" id="CAI50906">
        <property type="protein sequence ID" value="CAI50906"/>
        <property type="gene ID" value="NP_6252A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_6252A"/>
    <dbReference type="HOGENOM" id="CLU_149760_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="139" mass="14979" checksum="4BB0E17FD62677C0" modified="2005-11-08" version="1">MDAAELDEWTAPQEDDHQTVPCAACDAALQSPSRTTISFLLLDQLTIPLLGCSDHVEQFRSVCELTTEERAELLDHRPAGGINCPGCRQAGNRLQLPTLPVGRGAVAVLACESHQSEIIGRFQAGLQAQQSLTASLQTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM04</accession>
    <name>Q3IM04_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ribonucleoside-diphosphate reductase alpha subunit</fullName>
            <ecNumber evidence="3">1.17.4.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">nrdA1</name>
        <name evidence="3" type="ordered locus">NP_6168A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribonucleoside diphosphate reductase large chain family.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="3"/>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324463.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM04"/>
    <dbReference type="SMR" id="Q3IM04"/>
    <dbReference type="EnsemblBacteria" id="CAI50864">
        <property type="protein sequence ID" value="CAI50864"/>
        <property type="gene ID" value="NP_6168A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694597"/>
    <dbReference type="KEGG" id="nph:NP_6168A"/>
    <dbReference type="HOGENOM" id="CLU_000404_7_0_2"/>
    <dbReference type="OrthoDB" id="6188at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005971">
        <property type="term" value="C:ribonucleoside-diphosphate reductase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009263">
        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013350">
        <property type="entry name" value="RNR_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000788">
        <property type="entry name" value="RNR_lg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039718">
        <property type="entry name" value="Rrm1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02510">
        <property type="entry name" value="NrdE-prime"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006577">
        <property type="entry name" value="PRK09102.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11573">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11573:SF6">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02867">
        <property type="entry name" value="Ribonuc_red_lgC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01183">
        <property type="entry name" value="RIBORDTASEM1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51998">
        <property type="entry name" value="PFL-like glycyl radical enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribonucleotide reductase large subunit C-terminal" evidence="2">
        <location>
            <begin position="93"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Ribonucleotide reductase large subunit C-terminal" evidence="2">
        <location>
            <begin position="219"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="Ribonucleotide reductase large subunit C-terminal" evidence="2">
        <location>
            <begin position="390"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="564" mass="64457" checksum="EF19D0CCCAD6B030" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM09</accession>
    <name>Q3IM09_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_6158A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324458.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM09"/>
    <dbReference type="EnsemblBacteria" id="CAI50859">
        <property type="protein sequence ID" value="CAI50859"/>
        <property type="gene ID" value="NP_6158A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694631"/>
    <dbReference type="KEGG" id="nph:NP_6158A"/>
    <dbReference type="HOGENOM" id="CLU_126927_0_0_2"/>
    <dbReference type="OrthoDB" id="165318at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="152" mass="17724" checksum="DB350A8BB3230606" modified="2005-11-08" version="1">MSVPHLGGDPNANLYAQLKRDVLDRVPQITAVEYVPDDIEAKQLRATFDPARLDPPTGPDSPELTVKWYRQEPHDWFRINYTDPNTGFHAGWHQDEDHPDLGRTHFQYSVAETEDRWGITFEHETPSLILWEIVEELLEDVRPTYQYANEEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM17</accession>
    <name>Q3IM17_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">IS1341-type transposase ISNph12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">NP_6142A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10 11">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324450.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM17"/>
    <dbReference type="EnsemblBacteria" id="CAI50851">
        <property type="protein sequence ID" value="CAI50851"/>
        <property type="gene ID" value="NP_6142A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694623"/>
    <dbReference type="KEGG" id="nph:NP_6142A"/>
    <dbReference type="HOGENOM" id="CLU_032903_0_1_2"/>
    <dbReference type="OrthoDB" id="33505at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF11">
        <property type="entry name" value="RNA-GUIDED DNA ENDONUCLEASE RV2885C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="8">
        <location>
            <begin position="192"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="9">
        <location>
            <begin position="304"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="391"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="216"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI50851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="416" mass="47863" checksum="BECCF840C43EAB78" modified="2005-11-08" version="1">MWYDYRFCAYPDRTGVAAEAERHIDIHRQAYNHTRYEYNALDTDEDNIGSAYQHQKRLTEWKDEWSVFSEVHSKALQKTVERFYDNLSNLSEKKQNGHKVGWLKWKPPREYQSVTYSQSGFELKNTSGRTATLWLSKIGDIPIRYHRPIPDNATIKEVTLKKETTGEWYVTFGLEVEDTTLPEKPDVDDLDAEDCVGIDLGITNYIYTSDGDSVDWLNLSDEYERLRREQRNLSRKEHRSNNWKKQRREVAKVKRRIKRKVEDFQHKLTTWLVKTYDAVFVEDLNVSGMLQQRGNARNKQDAAWRGFINMLEYKGDLYGTHVVQVNPAGTTKECAECGVETEKPLWVREHSCPTCGFEADRDANASYNVLSRGLRELGLGQAEVTPVETATAVETDGGRSSSVPASRVIETGSLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM25</accession>
    <name>Q3IM25_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC-type transport system permease protein (Probable substrate phosphate/phosphonate)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">phnE2</name>
        <name evidence="9" type="synonym">abc18p1</name>
        <name evidence="9" type="ordered locus">NP_6122A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9 10">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324442.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM25"/>
    <dbReference type="EnsemblBacteria" id="CAI50841">
        <property type="protein sequence ID" value="CAI50841"/>
        <property type="gene ID" value="NP_6122A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694676"/>
    <dbReference type="KEGG" id="nph:NP_6122A"/>
    <dbReference type="HOGENOM" id="CLU_064254_1_1_2"/>
    <dbReference type="OrthoDB" id="252910at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015416">
        <property type="term" value="F:ABC-type phosphonate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005769">
        <property type="entry name" value="PhnE/PtxC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01097">
        <property type="entry name" value="PhnE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30043:SF1">
        <property type="entry name" value="ABC TRANSPORT SYSTEM PERMEASE PROTEIN P69"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30043">
        <property type="entry name" value="PHOSPHONATES TRANSPORT SYSTEM PERMEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="25"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="84"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI50841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="274" mass="29813" checksum="880F4AF6D6F34456" modified="2005-11-08" version="1">MTADTGHDDPDGRTWQRPTVFGSQLIKYAVYLLVVVFFAWNVFQLRISLDRLLLGFSEGQTLLTRMLTLEGVGRHEISVIISGTIETLAMSVVATIIGVAISIPIGFLAARNVSPLLSYLLGRGMITFSRAFHGLVFAIIAVKAFGFGPFAGVVTLVFTTVGFYSKLLADELEDIDQGQIDAIRATGGSRMDVILYGFLPQVLPRMVGLAIYRWDINIRSASIIGIVGAGGLGQVLLTSFERFDYGFSMAIIISIIAIVMAGEIISSTVRRRIQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMM5</accession>
    <name>IF2G_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Translation initiation factor 2 subunit gamma</fullName>
            <ecNumber evidence="1">3.6.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">aIF2-gamma</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">eIF-2-gamma</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">eif2g</name>
        <name type="ordered locus">NP_5084A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotrimer composed of an alpha, a beta and a gamma chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EIF2G subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.3" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324243.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMM5"/>
    <dbReference type="SMR" id="Q3IMM5"/>
    <dbReference type="STRING" id="348780.NP_5084A"/>
    <dbReference type="EnsemblBacteria" id="CAI50633">
        <property type="protein sequence ID" value="CAI50633"/>
        <property type="gene ID" value="NP_5084A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703269"/>
    <dbReference type="KEGG" id="nph:NP_5084A"/>
    <dbReference type="eggNOG" id="arCOG01563">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027154_0_1_2"/>
    <dbReference type="OrthoDB" id="7798at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01888">
        <property type="entry name" value="eIF2_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03688">
        <property type="entry name" value="eIF2_gamma_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15490">
        <property type="entry name" value="eIF2_gamma_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000065">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000075">
        <property type="entry name" value="Translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00119">
        <property type="entry name" value="eIF_2_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050543">
        <property type="entry name" value="eIF2G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015256">
        <property type="entry name" value="eIF2g_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044127">
        <property type="entry name" value="eIF2g_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044128">
        <property type="entry name" value="eIF2g_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022424">
        <property type="entry name" value="TIF2_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03680">
        <property type="entry name" value="eif2g_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003077">
        <property type="entry name" value="PRK04000.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42854">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42854:SF3">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09173">
        <property type="entry name" value="eIF2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000015792" description="Translation initiation factor 2 subunit gamma">
        <location>
            <begin position="1"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="tr-type G" evidence="1">
        <location>
            <begin position="7"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="G1" evidence="1">
        <location>
            <begin position="16"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="G2" evidence="1">
        <location>
            <begin position="44"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="G3" evidence="1">
        <location>
            <begin position="90"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="G4" evidence="1">
        <location>
            <begin position="146"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="G5" evidence="1">
        <location>
            <begin position="181"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="19"/>
            <end position="24"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="146"/>
            <end position="149"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="181"/>
            <end position="183"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00119"/>
        </source>
    </evidence>
    <sequence length="409" mass="43939" checksum="19FDAEFBDA40D46F" modified="2005-11-08" version="1">MTQKHRQPEVNIGLVGHVDHGKTTLVESLSGEWTDQHSEEMKRGISIRLGYADATFRECPELDEPDRYTVEETCPDGSESEHLRTVSFVDAPGHETLMATMLSGAAIMDGAVLVIAANEPVPRAQTEEHLMALDIIGIDNIVIAQNKIDLVDREQALDNYEAIEEFVDGTVAEDAPVVPISAQQDVNMDLLMQTIESEIPTPDRDPDADARLQVARSFDINRPGTTWEDLTGGVLGGSLTQGQLEADDDIEIKPGREIEEGGQSEYQPVETTVRSLQAGGEFVDTVTPGGLLGVGTGLDPSLTKGDALAGQVAGPPGSLPPTRESFTMDVELLDRVVGEDAEEIEPISTGEPLMLTVGTATTVGSVTSARDDECEVQLKRPVCAPDGSKIAINRRVGARWRLIGVGTLR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMX5</accession>
    <name>RL5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl5</name>
        <name type="ordered locus">NP_4880A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This is one of the proteins that bind and probably mediate the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits; this bridge is implicated in subunit movement. May contact the P site tRNA; the 5S rRNA and some of its associated proteins might help stabilize positioning of ribosome-bound tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit; contacts the 5S rRNA and probably tRNA. Forms a bridge to the 30S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324143.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMX5"/>
    <dbReference type="SMR" id="Q3IMX5"/>
    <dbReference type="STRING" id="348780.NP_4880A"/>
    <dbReference type="EnsemblBacteria" id="CAI50531">
        <property type="protein sequence ID" value="CAI50531"/>
        <property type="gene ID" value="NP_4880A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703146"/>
    <dbReference type="KEGG" id="nph:NP_4880A"/>
    <dbReference type="eggNOG" id="arCOG04092">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061015_3_0_2"/>
    <dbReference type="OrthoDB" id="372044at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1440.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01333_A">
        <property type="entry name" value="Ribosomal_uL5_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002132">
        <property type="entry name" value="Ribosomal_uL5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022804">
        <property type="entry name" value="Ribosomal_uL5_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031309">
        <property type="entry name" value="Ribosomal_uL5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_uL5_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057266">
        <property type="entry name" value="Ribosomal_uL5_euk/arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031310">
        <property type="entry name" value="Ribosomal_uL5_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003258">
        <property type="entry name" value="PRK04219.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11994">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00281">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00673">
        <property type="entry name" value="Ribosomal_L5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002161">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="RL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000243100" description="Large ribosomal subunit protein uL5">
        <location>
            <begin position="1"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="176" mass="19761" checksum="5DA349F47B6B2FF7" modified="2005-11-08" version="1">MSSEPDAGEFHEMREPTVEKVVVHMGVGRGGEPLAQAEEILEEIVEQQPVRTTAQKTIQDFEVREGDPIGTKATLRGEDAEEFLETALPLADIDRKQFDETGNFSFGVEEHTEFPSQEYDPQIGIYGLDVTVNLVRPGYRVHKRDKVSRSIPSNHRLTAEDAVTFLESNFDVEVSE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-28" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IPJ2</accession>
    <name>RNP2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease P protein component 2</fullName>
            <shortName evidence="1">RNase P component 2</shortName>
            <ecNumber evidence="1">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Pop5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rnp2</name>
        <name type="ordered locus">NP_3736A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Consists of a catalytic RNA component and at least 4-5 protein subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic/archaeal RNase P protein component 2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323576.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IPJ2"/>
    <dbReference type="SMR" id="Q3IPJ2"/>
    <dbReference type="STRING" id="348780.NP_3736A"/>
    <dbReference type="EnsemblBacteria" id="CAI49959">
        <property type="protein sequence ID" value="CAI49959"/>
        <property type="gene ID" value="NP_3736A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703113"/>
    <dbReference type="KEGG" id="nph:NP_3736A"/>
    <dbReference type="eggNOG" id="arCOG01365">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137733_0_0_2"/>
    <dbReference type="OrthoDB" id="19261at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3250">
        <property type="entry name" value="Ribonuclease P, Pop5 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00755">
        <property type="entry name" value="RNase_P_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002759">
        <property type="entry name" value="Pop5/Rpp14/Rnp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038085">
        <property type="entry name" value="Rnp2-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01900">
        <property type="entry name" value="RNase_P_Rpp14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160350">
        <property type="entry name" value="Rnp2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_1000148370" description="Ribonuclease P protein component 2">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00755"/>
        </source>
    </evidence>
    <sequence length="161" mass="17554" checksum="370AE862D8DD1AD3" modified="2005-11-08" version="1">MKHLPKHLRPRWRYLAVGLESWPDADIDRRAFQRELWFAAQNLIGDAGSAALDGSVLHFRFEDGDGEAVIRARRGEVDALRAVLATLSTVDGHPLGVVVRGVSGTVRACEEKYIRGPQEGTEERTVAFAGADRPAVVRNDRVTVELSGGPVGATALDIRDN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-09-11" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IQT2</accession>
    <name>RPO10_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo10</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit N</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo10</name>
        <name evidence="1" type="synonym">rpoN</name>
        <name type="ordered locus">NP_2842A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal Rpo10/eukaryotic RPB10 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323137.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IQT2"/>
    <dbReference type="SMR" id="Q3IQT2"/>
    <dbReference type="STRING" id="348780.NP_2842A"/>
    <dbReference type="EnsemblBacteria" id="CAI49512">
        <property type="protein sequence ID" value="CAI49512"/>
        <property type="gene ID" value="NP_2842A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703163"/>
    <dbReference type="KEGG" id="nph:NP_2842A"/>
    <dbReference type="eggNOG" id="arCOG04244">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143122_1_1_2"/>
    <dbReference type="OrthoDB" id="371754at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000335">
        <property type="entry name" value="DNA-directed RNA polymerase subunit N, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00250">
        <property type="entry name" value="RNApol_arch_Rpo10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023580">
        <property type="entry name" value="RNA_pol_su_RPB10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020789">
        <property type="entry name" value="RNA_pol_suN_Zn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000268">
        <property type="entry name" value="RPABC5/Rpb10"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003089">
        <property type="entry name" value="PRK04016.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23431:SF3">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23431">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC5 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01194">
        <property type="entry name" value="RNA_pol_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005653">
        <property type="entry name" value="RNA_pol_N/8_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46924">
        <property type="entry name" value="RNA polymerase subunit RPB10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01112">
        <property type="entry name" value="RNA_POL_N_8KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000304197" description="DNA-directed RNA polymerase subunit Rpo10">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00250"/>
        </source>
    </evidence>
    <sequence length="64" mass="7296" checksum="50E848C45591F6E7" modified="2005-11-08" version="1">MMVPVRCFTCGTVVGEHWGEFKERAREGDEDPAEVLDELGVERACCRRMLVSHKDLVDIVSPYQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-21" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IQT8</accession>
    <name>RS4_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein uS4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">30S ribosomal protein S4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps4</name>
        <name type="ordered locus">NP_2830A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="1">With S5 and S12 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Contacts protein S5. The interaction surface between S4 and S5 is involved in control of translational fidelity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323131.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IQT8"/>
    <dbReference type="SMR" id="Q3IQT8"/>
    <dbReference type="STRING" id="348780.NP_2830A"/>
    <dbReference type="EnsemblBacteria" id="CAI49506">
        <property type="protein sequence ID" value="CAI49506"/>
        <property type="gene ID" value="NP_2830A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703182"/>
    <dbReference type="KEGG" id="nph:NP_2830A"/>
    <dbReference type="eggNOG" id="arCOG04239">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089738_1_1_2"/>
    <dbReference type="OrthoDB" id="10429at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01306_A">
        <property type="entry name" value="Ribosomal_uS4_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_uS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022802">
        <property type="entry name" value="Ribosomal_uS4_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005710">
        <property type="entry name" value="Ribosomal_uS4_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_uS4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003139">
        <property type="entry name" value="PRK04051.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01018">
        <property type="entry name" value="uS4_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="30S 40S RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF5">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000228943" description="Small ribosomal subunit protein uS4">
        <location>
            <begin position="1"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="S4 RNA-binding" evidence="1">
        <location>
            <begin position="105"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="174" mass="19438" checksum="E883040E8A50EAB6" modified="2005-11-08" version="1">MALGSNTKFYETPNHPFQGERIAEEGDLLSRYGLVNKEELWRAQSELRDYRREARRLIGEAQGDTVAAAEAGSEFLARLKRIGVLDDEDSLDDILSLDVTDILERRLQTVAYRKGLGNTPQQARQFIAHGHVTVNGARVSAPSYKVDVAEEGEIEFDETSPLADDLHPERAEGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IQY8</accession>
    <name>Q3IQY8_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">1,4-dihydroxy-2-naphthoyl-CoA synthase</fullName>
            <shortName evidence="4">DHNA-CoA synthase</shortName>
            <ecNumber evidence="3 4">4.1.3.36</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">menB</name>
        <name evidence="5" type="ordered locus">NP_2730A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Converts o-succinylbenzoyl-CoA (OSB-CoA) to 1,4-dihydroxy-2-naphthoyl-CoA (DHNA-CoA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>2-succinylbenzoyl-CoA + H(+) = 1,4-dihydroxy-2-naphthoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:26562"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57364"/>
            <dbReference type="ChEBI" id="CHEBI:58897"/>
            <dbReference type="EC" id="4.1.3.36"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Quinol/quinone metabolism; 1,4-dihydroxy-2-naphthoate biosynthesis; 1,4-dihydroxy-2-naphthoate from chorismate: step 6/7.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Quinol/quinone metabolism; menaquinone biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the enoyl-CoA hydratase/isomerase family. MenB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.36" evidence="3 4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323081.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IQY8"/>
    <dbReference type="STRING" id="348780.NP_2730A"/>
    <dbReference type="EnsemblBacteria" id="CAI49456">
        <property type="protein sequence ID" value="CAI49456"/>
        <property type="gene ID" value="NP_2730A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702908"/>
    <dbReference type="KEGG" id="nph:NP_2730A"/>
    <dbReference type="eggNOG" id="arCOG00240">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_7_2"/>
    <dbReference type="OrthoDB" id="51339at2157"/>
    <dbReference type="UniPathway" id="UPA00079"/>
    <dbReference type="UniPathway" id="UPA01057">
        <property type="reaction ID" value="UER00167"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008935">
        <property type="term" value="F:1,4-dihydroxy-2-naphthoyl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009234">
        <property type="term" value="P:menaquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.12.10:FF:000003">
        <property type="entry name" value="1,4-dihydroxy-2-naphthoyl-CoA synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000003">
        <property type="entry name" value="1,4-dihydroxy-2-naphthoyl-CoA synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01934">
        <property type="entry name" value="MenB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010198">
        <property type="entry name" value="DHNA-CoA_synthase_MenB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01929">
        <property type="entry name" value="menB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006186">
        <property type="entry name" value="PRK08321.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43113:SF1">
        <property type="entry name" value="1,4-DIHYDROXY-2-NAPHTHOYL-COA SYNTHASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43113">
        <property type="entry name" value="NUCLEOSIDE-DIPHOSPHATE-SUGAR EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4 5" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0474">Menaquinone biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="in other chain" evidence="4">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="4">
        <location>
            <begin position="87"/>
            <end position="91"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="4">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="4">
        <location>
            <begin position="138"/>
            <end position="142"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="4">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="4">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalysis" evidence="4">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalysis" evidence="4">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalysis" evidence="4">
        <location>
            <position position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00066833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="295" mass="33145" checksum="1596D29B1DBB52A2" modified="2005-11-08" version="1">MVSELFDEDRWEPITDDFEDITYHRSTETGAVRIAFDRPEVRNAFRPETVDELYTALDHAKRQTDIGCVLLTGNGPSPKDDGWAFCSGGDQRIRGEAGYEYEDTPDAEQQDAPRLHILEVQRLIRHIPKPVIAVVPGWAVGGGHSLHVVCDMTLASEAHAKFLQTDPDVASFDAGFGSAYLARQIGHKKAREVFFLGKTYSADEAVEMGMANEAVPHEDLEDVAHEWAERILSKSPMAIRMLKYAFNMDSDGLVGQQVFAGEATRLGYMTTEAQEGRDAFNENRKPDFEQFDWHY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IR53</accession>
    <name>Y2600_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0145 protein NP_2600A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">NP_2600A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0145 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323016.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IR53"/>
    <dbReference type="SMR" id="Q3IR53"/>
    <dbReference type="STRING" id="348780.NP_2600A"/>
    <dbReference type="EnsemblBacteria" id="CAI49391">
        <property type="protein sequence ID" value="CAI49391"/>
        <property type="gene ID" value="NP_2600A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701503"/>
    <dbReference type="KEGG" id="nph:NP_2600A"/>
    <dbReference type="eggNOG" id="arCOG02287">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117144_1_2_2"/>
    <dbReference type="OrthoDB" id="59443at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.70">
        <property type="entry name" value="Hypothetical protein apc22750. Chain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00338">
        <property type="entry name" value="UPF0145"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035439">
        <property type="entry name" value="UPF0145_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002765">
        <property type="entry name" value="UPF0145_YbjQ-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34068:SF2">
        <property type="entry name" value="UPF0145 PROTEIN SCO3412"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34068">
        <property type="entry name" value="UPF0145 PROTEIN YBJQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01906">
        <property type="entry name" value="YbjQ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117782">
        <property type="entry name" value="YbjQ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000225855" description="UPF0145 protein NP_2600A">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00338"/>
        </source>
    </evidence>
    <sequence length="104" mass="11092" checksum="C211696FAE5CEB0A" modified="2005-11-08" version="1">MQTVSTETVPGHETVEALGIARGNTVEARNVGRDITQSIRNITGGELKAYSELLSKARDEALSRMEADAEEMGADAVVNVRLETSKVTDGGSEVIAYGTAVRLR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IRL7</accession>
    <name>SYK_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lysine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Lysyl-tRNA synthetase</fullName>
            <shortName evidence="1">LysRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lysS</name>
        <name type="ordered locus">NP_2270A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Lys) + L-lysine + ATP = L-lysyl-tRNA(Lys) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9696"/>
            <dbReference type="Rhea" id="RHEA-COMP:9697"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.6" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322853.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IRL7"/>
    <dbReference type="SMR" id="Q3IRL7"/>
    <dbReference type="STRING" id="348780.NP_2270A"/>
    <dbReference type="EnsemblBacteria" id="CAI49226">
        <property type="protein sequence ID" value="CAI49226"/>
        <property type="gene ID" value="NP_2270A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702894"/>
    <dbReference type="KEGG" id="nph:NP_2270A"/>
    <dbReference type="eggNOG" id="arCOG00485">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025562_1_0_2"/>
    <dbReference type="OrthoDB" id="6838at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004824">
        <property type="term" value="F:lysine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006430">
        <property type="term" value="P:lysyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.10">
        <property type="entry name" value="Lysine tRNA ligase, stem contact fold domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00177">
        <property type="entry name" value="Lys_tRNA_synth_class1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020751">
        <property type="entry name" value="aa-tRNA-synth_I_codon-bd_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008925">
        <property type="entry name" value="aa_tRNA-synth_I_cd-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002904">
        <property type="entry name" value="Lys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042078">
        <property type="entry name" value="Lys-tRNA-ligase_SC_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00467">
        <property type="entry name" value="lysS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37940">
        <property type="entry name" value="LYSINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37940:SF1">
        <property type="entry name" value="LYSINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01921">
        <property type="entry name" value="tRNA-synt_1f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48163">
        <property type="entry name" value="An anticodon-binding domain of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000225643" description="Lysine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="41"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="306"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00177"/>
        </source>
    </evidence>
    <sequence length="545" mass="60979" checksum="37D4488CC0FC26BB" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-01-10" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IS17</accession>
    <name>GPMI_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">2,3-bisphosphoglycerate-independent phosphoglycerate mutase</fullName>
            <shortName evidence="1">BPG-independent PGAM</shortName>
            <shortName evidence="1">Phosphoglyceromutase</shortName>
            <shortName evidence="1">iPGM</shortName>
            <ecNumber evidence="1">5.4.2.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gpmI</name>
        <name type="synonym">gpmA</name>
        <name type="ordered locus">NP_1964A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2-phosphoglycerate = (2R)-3-phosphoglycerate</text>
            <dbReference type="Rhea" id="RHEA:15901"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="EC" id="5.4.2.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the BPG-independent phosphoglycerate mutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.12" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322703.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IS17"/>
    <dbReference type="SMR" id="Q3IS17"/>
    <dbReference type="STRING" id="348780.NP_1964A"/>
    <dbReference type="EnsemblBacteria" id="CAI49073">
        <property type="protein sequence ID" value="CAI49073"/>
        <property type="gene ID" value="NP_1964A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702768"/>
    <dbReference type="KEGG" id="nph:NP_1964A"/>
    <dbReference type="eggNOG" id="arCOG03068">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026099_2_0_2"/>
    <dbReference type="OrthoDB" id="146005at2157"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00186"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004619">
        <property type="term" value="F:phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006007">
        <property type="term" value="P:glucose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16010">
        <property type="entry name" value="iPGM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1450.10:FF:000002">
        <property type="entry name" value="2,3-bisphosphoglycerate-independent phosphoglycerate mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1450.10">
        <property type="entry name" value="BPG-independent phosphoglycerate mutase, domain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01038">
        <property type="entry name" value="GpmI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011258">
        <property type="entry name" value="BPG-indep_PGM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006124">
        <property type="entry name" value="Metalloenzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036646">
        <property type="entry name" value="PGAM_B_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005995">
        <property type="entry name" value="Pgm_bpd_ind"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01307">
        <property type="entry name" value="pgm_bpd_ind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31637">
        <property type="entry name" value="2,3-BISPHOSPHOGLYCERATE-INDEPENDENT PHOSPHOGLYCERATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31637:SF0">
        <property type="entry name" value="2,3-BISPHOSPHOGLYCERATE-INDEPENDENT PHOSPHOGLYCERATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06415">
        <property type="entry name" value="iPGM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01676">
        <property type="entry name" value="Metalloenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001492">
        <property type="entry name" value="IPGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64158">
        <property type="entry name" value="2,3-Bisphosphoglycerate-independent phosphoglycerate mutase, substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000212243" description="2,3-bisphosphoglycerate-independent phosphoglycerate mutase">
        <location>
            <begin position="1"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="1">
        <location>
            <position position="59"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="149"/>
            <end position="150"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="254"/>
            <end position="257"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="394"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="398"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="435"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="436"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="452"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01038"/>
        </source>
    </evidence>
    <sequence length="506" mass="53807" checksum="1966D74E04FF6B85" modified="2005-11-08" version="1">MEAALVILDGWGLGDGDETDAVAAADTPAFDALAERGATGQLETHGRSVGLPDGQMGNSEVGHLNIGAGRVVKQDSTRVTDDIDAGVFADNEALARAFDHADDHDGRVHFMGLVSDGGVHSYQSHLHALVDLAAERGVEAVTHAFTDGRDTAPKSGVGFIEDLAATVEEAGTGDVATVCGRYYAMDRDENWERTKRAYDAIVHREAPHEADSAVAAVAASYDRGDTDEYVEPTLVSGTDAPLSADDAVVFFNFRADRARQLVRMLADIDPDWAFETDPPAAALVTMTEYDETFDLPVAYPPLAPEQPLGAVLAESDRTQLRMAESEKYAHVTYFLNGGREVAFEGERRDIVDSPDVPTYDEQPAMSAPELTDAAIDHIGADAPDVLVLNYANPDMVGHTGDFEAAKAAIEAVDEQLGRLESAIREAGGHLFVTADHGNADDMGTPEAPHTAHTTNPVPFVYCTPDGTDGGYTVRDGGTLADIAPTLLSTIGVDIPETMTGESLLER</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2014-04-16" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IS61</accession>
    <name>ARTA_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Archaeosortase A</fullName>
            <ecNumber evidence="1">3.4.22.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">artA</name>
        <name type="ordered locus">NP_1872A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2012" name="J. Bacteriol." volume="194" first="36" last="48">
            <title>Archaeosortases and exosortases are widely distributed systems linking membrane transit with posttranslational modification.</title>
            <authorList>
                <person name="Haft D.H."/>
                <person name="Payne S.H."/>
                <person name="Selengut J.D."/>
            </authorList>
            <dbReference type="PubMed" id="22037399"/>
            <dbReference type="DOI" id="10.1128/JB.06026-11"/>
        </citation>
        <scope>NOMENCLATURE</scope>
        <scope>GENE FAMILY</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Transpeptidase that recognizes and modifies its substrate by proteolytic cleavage of a sorting signal. Following cleavage, a covalent intermediate is formed via a thioester bond between the archaeosortase and its substrate, which is then transferred and covalently attached to the cell membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the exosortase/archaeosortase family. Archaeosortase A subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.-" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322659.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IS61"/>
    <dbReference type="STRING" id="348780.NP_1872A"/>
    <dbReference type="TCDB" id="9.B.297.1.4">
        <property type="family name" value="the archaeosortase/exosortase/rhomosortase (sortase) family"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI49027">
        <property type="protein sequence ID" value="CAI49027"/>
        <property type="gene ID" value="NP_1872A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701284"/>
    <dbReference type="KEGG" id="nph:NP_1872A"/>
    <dbReference type="eggNOG" id="arCOG04471">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065734_1_0_2"/>
    <dbReference type="OrthoDB" id="200496at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014522">
        <property type="entry name" value="ArtA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026392">
        <property type="entry name" value="Exo/Archaeosortase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019127">
        <property type="entry name" value="Exosortase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04178">
        <property type="entry name" value="exo_archaeo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04125">
        <property type="entry name" value="exosort_PGF_TRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09721">
        <property type="entry name" value="Exosortase_EpsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF025737">
        <property type="entry name" value="Cyco1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000428765" description="Archaeosortase A">
        <location>
            <begin position="1"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="260"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="1">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="218"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="D4GUZ4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="22037399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000305" key="5">
        <source>
            <dbReference type="PubMed" id="22037399"/>
        </source>
    </evidence>
    <sequence length="315" mass="34581" checksum="F2127C8F3806F78A" modified="2005-11-08" version="1">MSLLEAIAELHVIPYTDVLAWVVMAAFIAGVAADYRDNLLAARRLTAGAWWLFAVFWFVLIQHFAFVHRSVVQTVLILIAVPACLYVGWLVFAGRDSLLTLSRAVAFMTVIYLPFETSELARGLLIEAVAFQTATVIDALSLADGMEYMQDPDEGSTLMNTFWFPETGRASRVVFECTGIGAMSIFGGLIAAVNAPLRRKAVGIALSISIIWVLNIGRNVFIALANGYQWFAYSWLEGPIMALFGLTDPARVSFFVADRVLAQLLAVVALAGLAWFIARWVPELLDIAEELLSIVGIDVELHHPSVDRTDTDPAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ISY7</accession>
    <name>Q3ISY7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 8">Citrate synthase</fullName>
            <ecNumber evidence="6">2.3.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">citZ</name>
        <name evidence="9" type="ordered locus">NP_1314A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>oxaloacetate + acetyl-CoA + H2O = citrate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16845"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate metabolism; tricarboxylic acid cycle.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6 8">Belongs to the citrate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.16" evidence="6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322383.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ISY7"/>
    <dbReference type="STRING" id="348780.NP_1314A"/>
    <dbReference type="EnsemblBacteria" id="CAI48748">
        <property type="protein sequence ID" value="CAI48748"/>
        <property type="gene ID" value="NP_1314A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702612"/>
    <dbReference type="KEGG" id="nph:NP_1314A"/>
    <dbReference type="eggNOG" id="arCOG04237">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025068_2_1_2"/>
    <dbReference type="OrthoDB" id="21302at2157"/>
    <dbReference type="UniPathway" id="UPA00223"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036440">
        <property type="term" value="F:citrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06110">
        <property type="entry name" value="BSuCS-II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.580.10">
        <property type="entry name" value="Citrate Synthase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.230.10">
        <property type="entry name" value="Cytochrome P450-Terp, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011278">
        <property type="entry name" value="2-MeCitrate/Citrate_synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054872">
        <property type="entry name" value="Cit_synth_Halo_CitZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016142">
        <property type="entry name" value="Citrate_synth-like_lrg_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016143">
        <property type="entry name" value="Citrate_synth-like_sm_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024176">
        <property type="entry name" value="Citrate_synthase_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041301">
        <property type="entry name" value="Cit_synth_Halo_CitZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01800">
        <property type="entry name" value="cit_synth_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739">
        <property type="entry name" value="CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739:SF4">
        <property type="entry name" value="CITRATE SYNTHASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001369">
        <property type="entry name" value="Citrate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00143">
        <property type="entry name" value="CITRTSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="active site" evidence="7">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001369-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI48748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="378" mass="41598" checksum="C69F2A4EFE7CF283" modified="2005-11-08" version="1">MSDELKKGLEGVLVAESSLSYIDGDEGKLVYRGYTIDDLARNASFEEVVYLLWHGELPTADELGSFKSSMAEERHLDDGVQQLVRELAEADEEPMAALRSIVSSLSAYDPDADAEPTDEAANLRKGRRITAKIPTALAAFSRIREGDDPVEPREDLDHAANFLYMLNGEEPDEVLADTFDMALVLHADHGLNASTFSAMVTSSTLADLHAAITSAVGALSGSLHGGANQDVMEALLELDESGKEPVEWVEDRLDAGERVPGFGHRVYNVKDPRAKILSEESEALGEAAGTPQWHNYSTEIEEYLTEEKGLAPNVDFYSASTYYQMGIPVDIYTPIFAMSRVGGWIAHVLEQYDDNRLIRPRGRYVGPDDRTVTPIDER</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-04-29" modified="2026-01-28" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IT47</accession>
    <name>SYM_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methionine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Methionyl-tRNA synthetase</fullName>
            <shortName evidence="1">MetRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">metG</name>
        <name type="ordered locus">NP_1192A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of L-methionine to tRNA(Met). Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Met) + L-methionine + ATP = L-methionyl-tRNA(Met) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13481"/>
            <dbReference type="Rhea" id="RHEA-COMP:9667"/>
            <dbReference type="Rhea" id="RHEA-COMP:9698"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.10"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:13482"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.10" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322323.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IT47"/>
    <dbReference type="SMR" id="Q3IT47"/>
    <dbReference type="STRING" id="348780.NP_1192A"/>
    <dbReference type="EnsemblBacteria" id="CAI48687">
        <property type="protein sequence ID" value="CAI48687"/>
        <property type="gene ID" value="NP_1192A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702919"/>
    <dbReference type="KEGG" id="nph:NP_1192A"/>
    <dbReference type="eggNOG" id="arCOG00810">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009710_7_0_2"/>
    <dbReference type="OrthoDB" id="371856at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004825">
        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006431">
        <property type="term" value="P:methionyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07957">
        <property type="entry name" value="Anticodon_Ia_Met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00814">
        <property type="entry name" value="MetRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02800">
        <property type="entry name" value="tRNA_bind_EcMetRS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.20:FF:000001">
        <property type="entry name" value="Methionine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.20">
        <property type="entry name" value="Methionyl-tRNA synthetase, Zn-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00098">
        <property type="entry name" value="Met_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041872">
        <property type="entry name" value="Anticodon_Met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004495">
        <property type="entry name" value="Met-tRNA-synth_bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023458">
        <property type="entry name" value="Met-tRNA_ligase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014758">
        <property type="entry name" value="Met-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033911">
        <property type="entry name" value="MetRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029038">
        <property type="entry name" value="MetRS_Zn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00398">
        <property type="entry name" value="metG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001100">
        <property type="entry name" value="PRK00133.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765">
        <property type="entry name" value="METHIONINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765:SF1">
        <property type="entry name" value="METHIONINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19303">
        <property type="entry name" value="Anticodon_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01041">
        <property type="entry name" value="TRNASYNTHMET"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57770">
        <property type="entry name" value="Methionyl-tRNA synthetase (MetRS), Zn-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000331952" description="Methionine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA-binding" evidence="1">
        <location>
            <begin position="600"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="567"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="1">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="570"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="350"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="698" mass="77762" checksum="3F7F5E831BCD132D" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ITK1</accession>
    <name>DNLJ_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA ligase</fullName>
            <ecNumber evidence="1">6.5.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Polydeoxyribonucleotide synthase [NAD(+)]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ligA</name>
        <name type="ordered locus">NP_0882A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NAD(+) + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide.</text>
            <dbReference type="EC" id="6.5.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD-dependent DNA ligase family. LigA subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.2" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322168.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ITK1"/>
    <dbReference type="SMR" id="Q3ITK1"/>
    <dbReference type="STRING" id="348780.NP_0882A"/>
    <dbReference type="EnsemblBacteria" id="CAI48532">
        <property type="protein sequence ID" value="CAI48532"/>
        <property type="gene ID" value="NP_0882A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702885"/>
    <dbReference type="KEGG" id="nph:NP_0882A"/>
    <dbReference type="eggNOG" id="arCOG04754">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007764_2_1_2"/>
    <dbReference type="OrthoDB" id="213206at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003911">
        <property type="term" value="F:DNA ligase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17748">
        <property type="entry name" value="BRCT_DNA_ligase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00114">
        <property type="entry name" value="LIGANc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.610">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01588">
        <property type="entry name" value="DNA_ligase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041663">
        <property type="entry name" value="DisA/LigA_HHH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001679">
        <property type="entry name" value="DNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018239">
        <property type="entry name" value="DNA_ligase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033136">
        <property type="entry name" value="DNA_ligase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013839">
        <property type="entry name" value="DNAligase_adenylation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013840">
        <property type="entry name" value="DNAligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004150">
        <property type="entry name" value="NAD_DNA_ligase_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00575">
        <property type="entry name" value="dnlj"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005932">
        <property type="entry name" value="PRK07956.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010931">
        <property type="entry name" value="PRK14351.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389">
        <property type="entry name" value="CHROMOSOME TRANSMISSION FIDELITY FACTOR 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389:SF9">
        <property type="entry name" value="DNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01653">
        <property type="entry name" value="DNA_ligase_aden"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12826">
        <property type="entry name" value="HHH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14520">
        <property type="entry name" value="HHH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03120">
        <property type="entry name" value="OB_DNA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001604">
        <property type="entry name" value="LigA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00532">
        <property type="entry name" value="LIGANc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01055">
        <property type="entry name" value="DNA_LIGASE_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01056">
        <property type="entry name" value="DNA_LIGASE_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000313530" description="DNA ligase">
        <location>
            <begin position="1"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="1">
        <location>
            <begin position="607"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="active site" description="N6-AMP-lysine intermediate" evidence="1">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="56"/>
            <end position="60"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="104"/>
            <end position="105"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="338"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="429"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="432"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="445"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="451"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01588"/>
        </source>
    </evidence>
    <sequence length="691" mass="75706" checksum="586ED6C56413F9BC" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ITM1</accession>
    <name>Q3ITM1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Mrp-type sodium/proton antiporter system subunit E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">mrpE</name>
        <name evidence="8" type="synonym">tp49a</name>
        <name evidence="8" type="ordered locus">NP_0842A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322148.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ITM1"/>
    <dbReference type="STRING" id="348780.NP_0842A"/>
    <dbReference type="EnsemblBacteria" id="CAI48512">
        <property type="protein sequence ID" value="CAI48512"/>
        <property type="gene ID" value="NP_0842A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703327"/>
    <dbReference type="KEGG" id="nph:NP_0842A"/>
    <dbReference type="eggNOG" id="arCOG03099">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069531_0_0_2"/>
    <dbReference type="OrthoDB" id="85180at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002758">
        <property type="entry name" value="Cation_antiport_E"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009295">
        <property type="entry name" value="PRK12652.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34584">
        <property type="entry name" value="NA(+)/H(+) ANTIPORTER SUBUNIT E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34584:SF1">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER SUBUNIT E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01899">
        <property type="entry name" value="MNHE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="160"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="329"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="331"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="351"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI48512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="360" mass="37997" checksum="B10477D8C9A4325A" modified="2005-11-08" version="1">MEDAIMSVANDVLVPISPSTTLKQTINYAVEVANGAESGGSVHLVLTTPGHHVGGEGLNRDKELLARVEGYAAAAAEGSVSIEAKLLGTDEYFADPEDHFELLVRYVEEHGIERVVIDRSYSVDATAQPLQDIGAALAEAGIDYEVPAASGSWRPTGAELVRGSIIAVVAFGFYIALGGPTYTFALWTGAVTALIAAVLLRNVAFERTPQLGAALATVGRGLLFIPYMLWEITKANVQFAYVVLHPSLPIDPCLDRVDAAVGDGLSLTTFANSITLTPGTLTVDSVGNELLVHSLAPSAREDLINGLHERAVRFFFYGRKGLELPGPAARGDVEPLIRPSETKAEAITDGGTDEGGDADD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ITU5</accession>
    <name>SYFB_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phenylalanine--tRNA ligase beta subunit</fullName>
            <ecNumber evidence="1">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Phenylalanyl-tRNA synthetase beta subunit</fullName>
            <shortName evidence="1">PheRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pheT</name>
        <name type="ordered locus">NP_0694A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322074.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ITU5"/>
    <dbReference type="SMR" id="Q3ITU5"/>
    <dbReference type="STRING" id="348780.NP_0694A"/>
    <dbReference type="EnsemblBacteria" id="CAI48438">
        <property type="protein sequence ID" value="CAI48438"/>
        <property type="gene ID" value="NP_0694A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703026"/>
    <dbReference type="KEGG" id="nph:NP_0694A"/>
    <dbReference type="eggNOG" id="arCOG00412">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020279_3_0_2"/>
    <dbReference type="OrthoDB" id="10073at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009328">
        <property type="term" value="C:phenylalanine-tRNA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00769">
        <property type="entry name" value="PheRS_beta_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.40.10:FF:000003">
        <property type="entry name" value="Phenylalanine--tRNA ligase beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.40.10">
        <property type="entry name" value="Phenylalanyl-trna Synthetase, Chain B, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00284">
        <property type="entry name" value="Phe_tRNA_synth_beta2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005146">
        <property type="entry name" value="B3/B4_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045060">
        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004531">
        <property type="entry name" value="Phe-tRNA-synth_IIc_bsu_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020825">
        <property type="entry name" value="Phe-tRNA_synthase-like_B3/B4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022918">
        <property type="entry name" value="Phe_tRNA_ligase_beta2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041616">
        <property type="entry name" value="PheRS_beta_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005147">
        <property type="entry name" value="tRNA_synthase_B5-dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00471">
        <property type="entry name" value="pheT_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947:SF0">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE BETA CHAIN AND LEUCINE-RICH REPEAT-CONTAINING PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03484">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17759">
        <property type="entry name" value="tRNA_synthFbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00873">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00874">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51483">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000022429" description="Phenylalanine--tRNA ligase beta subunit">
        <location>
            <begin position="1"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="domain" description="B5" evidence="1">
        <location>
            <begin position="285"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="347"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="350"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="351"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00284"/>
        </source>
    </evidence>
    <sequence length="572" mass="62554" checksum="537FE1C6AA655573" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IUJ2</accession>
    <name>TRM1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA (guanine(26)-N(2))-dimethyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.216</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA 2,2-dimethylguanosine-26 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA(guanine-26,N(2)-N(2)) methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA(m(2,2)G26)dimethyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trm1</name>
        <name type="ordered locus">NP_0194A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Dimethylates a single guanine residue at position 26 of a number of tRNAs using S-adenosyl-L-methionine as donor of the methyl groups.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>guanosine(26) in tRNA + 2 S-adenosyl-L-methionine = N(2)-dimethylguanosine(26) in tRNA + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:43140"/>
            <dbReference type="Rhea" id="RHEA-COMP:10359"/>
            <dbReference type="Rhea" id="RHEA-COMP:10360"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74513"/>
            <dbReference type="EC" id="2.1.1.216"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. Trm1 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.216" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321827.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IUJ2"/>
    <dbReference type="SMR" id="Q3IUJ2"/>
    <dbReference type="STRING" id="348780.NP_0194A"/>
    <dbReference type="EnsemblBacteria" id="CAI48188">
        <property type="protein sequence ID" value="CAI48188"/>
        <property type="gene ID" value="NP_0194A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703360"/>
    <dbReference type="KEGG" id="nph:NP_0194A"/>
    <dbReference type="eggNOG" id="arCOG01219">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010862_5_1_2"/>
    <dbReference type="OrthoDB" id="372177at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160104">
        <property type="term" value="F:tRNA (guanine(26)-N2)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002940">
        <property type="term" value="P:tRNA N2-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.70">
        <property type="entry name" value="N2,N2-dimethylguanosine tRNA methyltransferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00290">
        <property type="entry name" value="tRNA_dimethyltr_TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002905">
        <property type="entry name" value="Trm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022923">
        <property type="entry name" value="TRM1_arc_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042296">
        <property type="entry name" value="tRNA_met_Trm1_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003327">
        <property type="entry name" value="PRK04338.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00308">
        <property type="entry name" value="TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631">
        <property type="entry name" value="N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631:SF3">
        <property type="entry name" value="TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02005">
        <property type="entry name" value="TRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51626">
        <property type="entry name" value="SAM_MT_TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000259377" description="tRNA (guanine(26)-N(2))-dimethyltransferase">
        <location>
            <begin position="1"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="Trm1 methyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="366" mass="39710" checksum="75186BB6479872B8" modified="2005-11-08" version="1">MEVSEGSVTVEVPEERHGASEGSGEGVFYNPVQELNRDITAAVLRTVSDDCDEYLDAMAASGIRSVRAAAAGYDVTACDIDDDAVALTRRNLDRNGVDGTVHHRNVNAHMHESAHDVVDLDPFGTPMAFADAAVRSAGEYLCVTATDTAPLCGAHFESGVRSYDAVPRNTEFHAEMGLRVLLSALVRTAARHDIAAEPVLSHVTSHYVRTYLRVDSGARAADALLEHLGYIDHCQRCLWRDHERGRVPDPTTTCPHCGQSTWTAGPVWLGPAHDAAFVDDVAGAIPDSFGTADKARDLLSTVATELHQPTHYDQHKLYKRWNEPNIAMDDFLAALRAAGHEASRTHYGGTTFKTDADVADIRNAVE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-09-11" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IUJ7</accession>
    <name>PFDA_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Prefoldin subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">GimC subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pfdA</name>
        <name type="ordered locus">NP_0184A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Molecular chaperone capable of stabilizing a range of proteins. Seems to fulfill an ATP-independent, HSP70-like function in archaeal de novo protein folding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterohexamer of two alpha and four beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the prefoldin subunit alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321822.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IUJ7"/>
    <dbReference type="SMR" id="Q3IUJ7"/>
    <dbReference type="STRING" id="348780.NP_0184A"/>
    <dbReference type="EnsemblBacteria" id="CAI48183">
        <property type="protein sequence ID" value="CAI48183"/>
        <property type="gene ID" value="NP_0184A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703017"/>
    <dbReference type="KEGG" id="nph:NP_0184A"/>
    <dbReference type="eggNOG" id="arCOG01341">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091867_1_3_2"/>
    <dbReference type="OrthoDB" id="10045at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00584">
        <property type="entry name" value="Prefoldin_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00308">
        <property type="entry name" value="PfdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011599">
        <property type="entry name" value="PFD_alpha_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004127">
        <property type="entry name" value="Prefoldin_subunit_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00293">
        <property type="entry name" value="prefoldin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12674">
        <property type="entry name" value="PREFOLDIN SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12674:SF2">
        <property type="entry name" value="PREFOLDIN SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02996">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000300768" description="Prefoldin subunit alpha">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="120"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="133"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="151" mass="16555" checksum="ACDD4C27D1977626" modified="2005-11-08" version="1">MSLGGGGGGGAMEQIQQQLQALEQEKQAIQAEIENVRDEQSEIDEAIEAIETLETGATVQVPLGGDAYVRATIEDMDEVVVTLGGGYAAERDSEGAVESLERKKETLDDRIEELEGEIETVEEETASLEEKAQQAQQQQMQQLQQMQQEDE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IUS0</accession>
    <name>MSRA_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptide methionine sulfoxide reductase MsrA</fullName>
            <shortName evidence="1">Protein-methionine-S-oxide reductase</shortName>
            <ecNumber evidence="1">1.8.4.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Peptide-methionine (S)-S-oxide reductase</fullName>
            <shortName evidence="1">Peptide Met(O) reductase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">msrA</name>
        <name type="ordered locus">NP_0038A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-methionyl-[protein] + [thioredoxin]-disulfide + H2O = L-methionyl-(S)-S-oxide-[protein] + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:14217"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12315"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:44120"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[thioredoxin]-disulfide + L-methionine + H2O = L-methionine (S)-S-oxide + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:19993"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58772"/>
            <dbReference type="EC" id="1.8.4.11"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MsrA Met sulfoxide reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.11" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321749.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IUS0"/>
    <dbReference type="SMR" id="Q3IUS0"/>
    <dbReference type="STRING" id="348780.NP_0038A"/>
    <dbReference type="EnsemblBacteria" id="CAI48110">
        <property type="protein sequence ID" value="CAI48110"/>
        <property type="gene ID" value="NP_0038A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702945"/>
    <dbReference type="KEGG" id="nph:NP_0038A"/>
    <dbReference type="eggNOG" id="arCOG02816">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031040_10_0_2"/>
    <dbReference type="OrthoDB" id="7150at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033744">
        <property type="term" value="F:L-methionine:thioredoxin-disulfide S-oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008113">
        <property type="term" value="F:peptide-methionine (S)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1060.10">
        <property type="entry name" value="Peptide methionine sulphoxide reductase MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01401">
        <property type="entry name" value="MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002569">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036509">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00401">
        <property type="entry name" value="msrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43774">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43774:SF1">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE MSRA 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01625">
        <property type="entry name" value="PMSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55068">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000145423" description="Peptide methionine sulfoxide reductase MsrA">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01401"/>
        </source>
    </evidence>
    <sequence length="178" mass="20112" checksum="A828428F7B51A09B" modified="2005-11-08" version="1">MTATATLGGGCFWCVEAAFEELDGVESVTSGYAGGHTEDPTYREVCSGNTGHAEVVQVAYDSDVLAYEDLLEVFFTVHDPTQLNRQGPDVGSQYRSIVLYHDDTQRELAEAYIESLDEAGEYDDDIVTEIERLETFYRAEENHQNYFEKNPQDAYCRMHAQPKIETVREAFAEKVDSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-04-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1R4ACJ0</accession>
    <name>A0A1R4ACJ0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_6118A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="SJL05866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1R4ACJ0"/>
    <dbReference type="EnsemblBacteria" id="SJL05866">
        <property type="protein sequence ID" value="SJL05866"/>
        <property type="gene ID" value="NP_6118A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_6118A"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="SJL05866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="42" mass="4809" checksum="2F3DE0AA6AA86A0D" modified="2017-04-12" version="1">MIDENPIESAAADVDGASPPPAPEEYSEPYRFEYRMYVFEPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ET97</accession>
    <name>A0A1U7ET97_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Enoyl-CoA hydratase</fullName>
            <ecNumber evidence="1">4.2.1.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fadA6</name>
        <name evidence="1" type="ordered locus">NP_0032A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.2.1.17" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321746.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ET97"/>
    <dbReference type="STRING" id="348780.NP_0032A"/>
    <dbReference type="DNASU" id="3702683"/>
    <dbReference type="EnsemblBacteria" id="CAI48107">
        <property type="protein sequence ID" value="CAI48107"/>
        <property type="gene ID" value="NP_0032A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702683"/>
    <dbReference type="KEGG" id="nph:NP_0032A"/>
    <dbReference type="eggNOG" id="arCOG00241">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_2_2"/>
    <dbReference type="OrthoDB" id="27846at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43459:SF1">
        <property type="entry name" value="EG:BACN32G11.4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43459">
        <property type="entry name" value="ENOYL-COA HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="255" mass="27017" checksum="2C1DFA87350930B1" modified="2017-05-10" version="1">MSVELTRDDAVATITISNPDRKNAIDAPTARQMADHVADVAADDSVRCVVVTGEGEAFCSGLDLAGDMGGGDVVAELDAGLNAIVRRLMRMEKPTVAKVPGPAVGAGASIASACDFVYPVEGTFFEWGFTNIGLAPDTGATYVLPRLVGVRQALELLITGRRVAADEAVNLGIANETVPESDFESVVADRAAMLAERPTLAVGAAKRLVYRSHQRSMEETLREEALAQSALMESDDFMEGVAAFLADREPEFEGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETH4</accession>
    <name>A0A1U7ETH4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">MgtE family transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">tp48b</name>
        <name evidence="11" type="ordered locus">NP_0222A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC41A transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321841.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETH4"/>
    <dbReference type="STRING" id="348780.NP_0222A"/>
    <dbReference type="EnsemblBacteria" id="CAI48202">
        <property type="protein sequence ID" value="CAI48202"/>
        <property type="gene ID" value="NP_0222A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703326"/>
    <dbReference type="KEGG" id="nph:NP_0222A"/>
    <dbReference type="eggNOG" id="arCOG00624">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111003_0_0_2"/>
    <dbReference type="OrthoDB" id="203810at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.20">
        <property type="entry name" value="SLC41 divalent cation transporters, integral membrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006667">
        <property type="entry name" value="SLC41_membr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036739">
        <property type="entry name" value="SLC41_membr_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045349">
        <property type="entry name" value="SLC41A1-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16228">
        <property type="entry name" value="DIVALENT CATION TRANSPORTER SOLUTE CARRIER FAMILY 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16228:SF7">
        <property type="entry name" value="SLC41A_MGTE INTEGRAL MEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01769">
        <property type="entry name" value="MgtE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161093">
        <property type="entry name" value="MgtE membrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="17"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="93"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="129"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="SLC41A/MgtE integral membrane" evidence="10">
        <location>
            <begin position="57"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI48202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="216" mass="22660" checksum="18D732D148514885" modified="2017-05-10" version="1">MVAPSGVGSLNTWSTRAIVLTMAPILIGLSILQMVSGAVLETLEETYLGNPTLLVLVPVMIDMGGNLGCILASRLSTRLHLGVLEFDIRDTALWTNVIAILGLAATIFTILGFAAYGVGQFITGEPLPLFDLLLISLLSGMSLAVLAILLAVSATYVSYRMGLDPDDTTIPVVTNVADICGVLILSGVAILVLDQDFGDGIEETTAALVEFVVLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETI8</accession>
    <name>A0A1U7ETI8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Probable amidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0256A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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    <dbReference type="EnsemblBacteria" id="CAI48219">
        <property type="protein sequence ID" value="CAI48219"/>
        <property type="gene ID" value="NP_0256A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700805"/>
    <dbReference type="KEGG" id="nph:NP_0256A"/>
    <dbReference type="eggNOG" id="arCOG01717">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="359273at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
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        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT A, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="26"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="477" mass="48913" checksum="1EC6B43A2F25E73B" modified="2017-05-10" version="1">MVNLTGRTATALAAAVREGELSAADLASASLDRIDATTDLNAYVTVLDSAARERAAAIDDGEASGPLAGVPVAIKDLRSRKAGVRNTMGLAALSDNIAESDAIVTERLEAAGANIVGTTNTPALGHTIKTDNRLVGATPTPFDENRSAGGSSGGSAAALAAGTVRLATGSDIGGSLRVPASCCNVIGLKPTLGVVPERGGTDAFSPNSPFFVGGPMARTAEETALGLSVLAGHDSRDPYSVPLGETEYLAATERPTESLSVAYSPDLDLQPVAPAVRETVGKAVSDLEAAGAAVETTAVSLPPYEELSMAYVTQVGAFFAAFAQQVEAEYGVDFETADIEPTVEATISLADGLEASDERKQNVPRTAAYDGIEAALAGHDVLITPTLTVPPYGKRLADGYPTEIDGETVRGVPTDAMLTWVFNLTGHPAASVPAGFTDDGLPVGLQVVGRRFGEADVLAAAAALQRVRPWTDAYPDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETP4</accession>
    <name>A0A1U7ETP4_NATPD</name>
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        <submittedName>
            <fullName evidence="2">HTH domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0378A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETP4"/>
    <dbReference type="STRING" id="348780.NP_0378A"/>
    <dbReference type="EnsemblBacteria" id="CAI48280">
        <property type="protein sequence ID" value="CAI48280"/>
        <property type="gene ID" value="NP_0378A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700835"/>
    <dbReference type="KEGG" id="nph:NP_0378A"/>
    <dbReference type="eggNOG" id="arCOG02866">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_172273_0_0_2"/>
    <dbReference type="OrthoDB" id="259485at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055547">
        <property type="entry name" value="DUF7123"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23438">
        <property type="entry name" value="DUF7123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7123" evidence="1">
        <location>
            <begin position="3"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="74" mass="8564" checksum="0295C6A426578874" modified="2017-05-10" version="1">MSELSEEDERILAHLRERAKGGDRYFRAKHIADAIDLTAKQVGARLPRLAEQSEDVDIEKWSRARSTTWRVTPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETP6</accession>
    <name>A0A1U7ETP6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Translation initiation factor aIF-2 beta subunit / probable RNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">tif2b2</name>
        <name evidence="6" type="ordered locus">NP_0406A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2-beta/eIF-5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETP6"/>
    <dbReference type="STRING" id="348780.NP_0406A"/>
    <dbReference type="EnsemblBacteria" id="CAI48294">
        <property type="protein sequence ID" value="CAI48294"/>
        <property type="gene ID" value="NP_0406A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700845"/>
    <dbReference type="KEGG" id="nph:NP_0406A"/>
    <dbReference type="eggNOG" id="arCOG01640">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026663_3_0_2"/>
    <dbReference type="OrthoDB" id="38099at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045196">
        <property type="entry name" value="IF2/IF5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002735">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016189">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016190">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008993">
        <property type="entry name" value="PRK12336.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001:SF3">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01873">
        <property type="entry name" value="eIF-5_eIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00653">
        <property type="entry name" value="eIF2B_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100966">
        <property type="entry name" value="Translation initiation factor 2 beta, aIF2beta, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75689">
        <property type="entry name" value="Zinc-binding domain of translation initiation factor 2 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GATA-type" evidence="4">
        <location>
            <begin position="95"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="5">
        <location>
            <begin position="146"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI48294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="203" mass="21842" checksum="70EF6B38A7DD185A" modified="2017-05-10" version="1">MEYEASLDRALADVPDIASSGERLSVPDAAAQTDGAFTRFTNLSAVADALNRDADHIHRFVQQTLATSGKLSEGVGRYNGDFDDRDFEAAVDDYADEYVRCSECGLPDTRLVQEDQTLMLRCDACGAFRPVSKNTGSSSTQTQDDDVEAGNTYTVKVTDTGRKGDGVAHRGDYTIFVPGASEGDVVDVYIENTSGTLAFSRIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETQ5</accession>
    <name>A0A1U7ETQ5_NATPD</name>
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        <submittedName>
            <fullName evidence="2">Rhodanese domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0400A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321929.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETQ5"/>
    <dbReference type="STRING" id="348780.NP_0400A"/>
    <dbReference type="EnsemblBacteria" id="CAI48291">
        <property type="protein sequence ID" value="CAI48291"/>
        <property type="gene ID" value="NP_0400A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700843"/>
    <dbReference type="KEGG" id="nph:NP_0400A"/>
    <dbReference type="eggNOG" id="arCOG02021">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089574_13_3_2"/>
    <dbReference type="OrthoDB" id="3168at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00158">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050229">
        <property type="entry name" value="GlpE_sulfurtransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43031">
        <property type="entry name" value="FAD-DEPENDENT OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43031:SF1">
        <property type="entry name" value="PYRIDINE NUCLEOTIDE-DISULPHIDE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="15"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="121" mass="13590" checksum="32AC006D4AD82938" modified="2017-05-10" version="1">MDAIRPPELQRRLESSDPPVILDIRPREAYQQSAIEGSHNVPVYDDLRSGNEGSLRDRLDEVPDGEVVVVCKQGIVAKRATELLRDAGREAETLAGGMGGWRGYRNGTFGYKLRSLLWRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETT5</accession>
    <name>A0A1U7ETT5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KEOPS complex subunit Cgi121</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cgi121</name>
        <name evidence="3" type="ordered locus">NP_0490A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CGI121/TPRKB family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321972.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETT5"/>
    <dbReference type="STRING" id="348780.NP_0490A"/>
    <dbReference type="EnsemblBacteria" id="CAI48336">
        <property type="protein sequence ID" value="CAI48336"/>
        <property type="gene ID" value="NP_0490A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700873"/>
    <dbReference type="KEGG" id="nph:NP_0490A"/>
    <dbReference type="eggNOG" id="arCOG02197">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103619_0_0_2"/>
    <dbReference type="OrthoDB" id="69587at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2380.10">
        <property type="entry name" value="CGI121/TPRKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013926">
        <property type="entry name" value="CGI121/TPRKB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036504">
        <property type="entry name" value="CGI121/TPRKB_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011528">
        <property type="entry name" value="NERD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016799">
        <property type="entry name" value="UCP022062"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011465">
        <property type="entry name" value="PRK14886.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08617">
        <property type="entry name" value="CGI-121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF022062">
        <property type="entry name" value="UCP022062"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143870">
        <property type="entry name" value="PF0523-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50965">
        <property type="entry name" value="NERD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NERD" evidence="2">
        <location>
            <begin position="115"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="168" mass="17986" checksum="891907367D145B79" modified="2017-05-10" version="1">MRVVEGIADIGGETAPDVGSFVQFLDEVGTDHGVTVQAFDARCIVSRRHLERAVELADRERSRGAAIARDRGVEIMLYAAGRRQIDRALEIGVSEGETSTVVVVSGDGDEAAAADAVADSLLPAETLGNYDRDRVRSFYDIGVAELEATTASLEALVLERVALLVVER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETZ2</accession>
    <name>A0A1U7ETZ2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">NP_0622A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322038.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETZ2"/>
    <dbReference type="STRING" id="348780.NP_0622A"/>
    <dbReference type="EnsemblBacteria" id="CAI48402">
        <property type="protein sequence ID" value="CAI48402"/>
        <property type="gene ID" value="NP_0622A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700908"/>
    <dbReference type="KEGG" id="nph:NP_0622A"/>
    <dbReference type="eggNOG" id="arCOG02329">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_58_2"/>
    <dbReference type="OrthoDB" id="8127at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00156">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013656">
        <property type="entry name" value="PAS_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052162">
        <property type="entry name" value="Sensor_kinase/Photoreceptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43304:SF1">
        <property type="entry name" value="PAC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43304">
        <property type="entry name" value="PHYTOCHROME-LIKE PROTEIN CPH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08448">
        <property type="entry name" value="PAS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Response regulatory" evidence="9">
        <location>
            <begin position="6"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="10">
        <location>
            <begin position="145"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="11">
        <location>
            <begin position="219"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="10">
        <location>
            <begin position="273"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="11">
        <location>
            <begin position="346"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="8">
        <location>
            <begin position="408"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="6">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI48402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="598" mass="67047" checksum="16E892D6CD5DA60D" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU75</accession>
    <name>A0A1U7EU75_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Transcription elongation factor Spt5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 8" type="primary">spt5</name>
        <name evidence="8" type="synonym">nusG</name>
        <name evidence="8" type="ordered locus">NP_0814A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Stimulates transcription elongation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterodimer composed of Spt4 and Spt5. Interacts with RNA polymerase (RNAP).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPT5 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the archaeal Spt5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322134.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU75"/>
    <dbReference type="STRING" id="348780.NP_0814A"/>
    <dbReference type="EnsemblBacteria" id="CAI48498">
        <property type="protein sequence ID" value="CAI48498"/>
        <property type="gene ID" value="NP_0814A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703001"/>
    <dbReference type="KEGG" id="nph:NP_0814A"/>
    <dbReference type="eggNOG" id="arCOG01920">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113589_0_0_2"/>
    <dbReference type="OrthoDB" id="371863at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006354">
        <property type="term" value="P:DNA-templated transcription elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06091">
        <property type="entry name" value="KOW_NusG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09887">
        <property type="entry name" value="NGN_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.940">
        <property type="entry name" value="NusG, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00950">
        <property type="entry name" value="Spt5_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005100">
        <property type="entry name" value="NGN-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006645">
        <property type="entry name" value="NGN-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036735">
        <property type="entry name" value="NGN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011590">
        <property type="entry name" value="Spt5_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00405">
        <property type="entry name" value="KOW_elon_Spt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03439">
        <property type="entry name" value="Spt5-NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00738">
        <property type="entry name" value="NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NusG-like N-terminal" evidence="6">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="7">
        <location>
            <begin position="87"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="NCBIfam" id="TIGR00405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI48498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="146" mass="15975" checksum="A391A452BF683A37" modified="2017-05-10" version="1">MAIYSVKTTASQERTVADMLISREEEGIHATLAPDALTSYVMVEADDTAAISRALEDIPHARTLVSEQPSPFSEVEHFLSPKPDVEGIAEGDIVELIAGPFKGEKAQVQRIDEGKDQVTVELYEATVPIPVTVRGDQIRVLDSEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU85</accession>
    <name>A0A1U7EU85_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Mrp-type sodium/proton antiporter system subunit B2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">mrpB2</name>
        <name evidence="9" type="synonym">tp49e</name>
        <name evidence="9" type="ordered locus">NP_0834A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322144.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU85"/>
    <dbReference type="STRING" id="348780.NP_0834A"/>
    <dbReference type="EnsemblBacteria" id="CAI48508">
        <property type="protein sequence ID" value="CAI48508"/>
        <property type="gene ID" value="NP_0834A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703331"/>
    <dbReference type="KEGG" id="nph:NP_0834A"/>
    <dbReference type="eggNOG" id="arCOG03079">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101659_0_0_2"/>
    <dbReference type="OrthoDB" id="19265at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050622">
        <property type="entry name" value="CPA3_antiporter_subunitB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007182">
        <property type="entry name" value="MnhB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33932">
        <property type="entry name" value="NA(+)/H(+) ANTIPORTER SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33932:SF4">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04039">
        <property type="entry name" value="MnhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="47"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Na+/H+ antiporter MnhB subunit-related protein" evidence="8">
        <location>
            <begin position="40"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI48508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="184" mass="18657" checksum="DB1CBC2C1948C28A" modified="2017-05-10" version="1">MNGDSEAPTTEIERSLGDDAGSEAIPDGGTPNTTDAQSPVVKTVSRILTPFVVAFGVYLTLFGTSLPGGAFQGGVVMASAIVLIGLGFGFDPTRRWLDPRGLTGLFVLGSFLFGGIAMAAVGLGGTVLELFVFPLSVENMVKLVEVAIAALVSGVITGLVIWLAAGYETGGKGGTSDSNGDDDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU96</accession>
    <name>A0A1U7EU96_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0886A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322170.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU96"/>
    <dbReference type="STRING" id="348780.NP_0886A"/>
    <dbReference type="EnsemblBacteria" id="CAI48534">
        <property type="protein sequence ID" value="CAI48534"/>
        <property type="gene ID" value="NP_0886A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700988"/>
    <dbReference type="KEGG" id="nph:NP_0886A"/>
    <dbReference type="HOGENOM" id="CLU_2433952_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="90" mass="8816" checksum="9D87C711774C0BA8" modified="2017-05-10" version="1">MVTALLAFAAIAAACGIAVYLASDTRPLFRAVVVFGGLLATPYLAFYLTGMALLGGLPAPVAAVLVVLLGVGLYLVAGGSIPGHNTGAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUE3</accession>
    <name>A0A1U7EUE3_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Adenosylhomocysteinase</fullName>
            <ecNumber evidence="4">3.13.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">S-adenosyl-L-homocysteine hydrolase</fullName>
            <shortName evidence="4">AdoHcyase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4 8" type="primary">ahcY</name>
        <name evidence="8" type="ordered locus">NP_0968A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May play a key role in the regulation of the intracellular concentration of adenosylhomocysteine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine</text>
            <dbReference type="Rhea" id="RHEA:21708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="3.13.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4 5">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
        <text evidence="4 5">Binds 1 NAD(+) per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 6">Belongs to the adenosylhomocysteinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.13.2.1" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322210.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUE3"/>
    <dbReference type="STRING" id="348780.NP_0968A"/>
    <dbReference type="EnsemblBacteria" id="CAI48575">
        <property type="protein sequence ID" value="CAI48575"/>
        <property type="gene ID" value="NP_0968A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701014"/>
    <dbReference type="KEGG" id="nph:NP_0968A"/>
    <dbReference type="eggNOG" id="arCOG04137">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025194_2_1_2"/>
    <dbReference type="OrthoDB" id="8479at2157"/>
    <dbReference type="UniPathway" id="UPA00314">
        <property type="reaction ID" value="UER00076"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004013">
        <property type="term" value="F:adenosylhomocysteinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071269">
        <property type="term" value="P:L-homocysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033353">
        <property type="term" value="P:S-adenosylmethionine cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00401">
        <property type="entry name" value="SAHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1480">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00563">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042172">
        <property type="entry name" value="Adenosylhomocyst_ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000043">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015878">
        <property type="entry name" value="Ado_hCys_hydrolase_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020082">
        <property type="entry name" value="S-Ado-L-homoCys_hydrolase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00936">
        <property type="entry name" value="ahcY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004005">
        <property type="entry name" value="PRK05476.2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420:SF0">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05221">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00670">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001109">
        <property type="entry name" value="Ad_hcy_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00996">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00997">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00738">
        <property type="entry name" value="ADOHCYASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00739">
        <property type="entry name" value="ADOHCYASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 4 5" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S-adenosyl-L-homocysteine hydrolase NAD binding" evidence="7">
        <location>
            <begin position="191"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 5">
        <location>
            <begin position="157"/>
            <end position="159"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="220"/>
            <end position="225"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="222"/>
            <end position="227"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 5">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 5">
        <location>
            <begin position="299"/>
            <end position="301"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 5">
        <location>
            <position position="346"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="355"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001109-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI48575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="426" mass="46149" checksum="159B601FECC6BA62" modified="2017-05-10" version="1">MSQPTITEQLDEPRVARESGRKKIEWAREHMPIMTAIAEEFNAEQPLDGEVVAMAMHVEAKTAVLTEVLAEAGAEVAITGCNPLSTHDDVSAALDANDAITSYAKRGVDDDAYYAAIEATLDHEPTVTVDDGGDLVMAIHEDYPELIDSIVGGCEETTTGVHRLRAMDEDGELKYPMFAVNDTPMKRLFDNVHGTGEASLSSIAMTTNLSFAGKTVVVSGYGYCGRGVAKKAAGQNAHVVVTEVEPRRALEAHMEGYEVCPMDEAAAKGDIFVTTTGNRDVIVKEHFERMSDGVVLANAGHFNVEIDLDALDDLAVSREEVRDGVEEFKLDDGRRINVLADGRLVNLAAPVALGHPVEVMDQSFGIQAVSVREMVENTDEYGAGVHEVPDELDKEVAEIKLDAEGIDIDDLSSEQAEYMDSWQHGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUF5</accession>
    <name>A0A1U7EUF5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF1684 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0958A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322206.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUF5"/>
    <dbReference type="STRING" id="348780.NP_0958A"/>
    <dbReference type="DNASU" id="3701011"/>
    <dbReference type="EnsemblBacteria" id="CAI48570">
        <property type="protein sequence ID" value="CAI48570"/>
        <property type="gene ID" value="NP_0958A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701011"/>
    <dbReference type="KEGG" id="nph:NP_0958A"/>
    <dbReference type="eggNOG" id="arCOG04570">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090976_1_0_2"/>
    <dbReference type="OrthoDB" id="334216at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1680">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012467">
        <property type="entry name" value="DUF1684"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41913">
        <property type="entry name" value="DUF1684 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41913:SF1">
        <property type="entry name" value="DUF1684 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07920">
        <property type="entry name" value="DUF1684"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="189" mass="21759" checksum="81A8D35BB98EF1B1" modified="2017-05-10" version="1">MEFDVDAWRAELTEYREQKDEQFATARSSPLPPEKRESFDGLDYFEPNPDYRVAATVEVDDSDETITMETTAEGERLYERVARLTFEVPDASDNRDEHTLVAYQRVDQRGDGSLFVPFRDKTTGQQTYPAGRYMELHYEGDLEDGATVPLDFNLAYNPFCAYSDAYECPLPPQENWLEIVVPAGERDDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUG2</accession>
    <name>A0A1U7EUG2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">5,10-methylenetetrahydromethanopterin reductase</fullName>
            <ecNumber evidence="4">1.5.98.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Coenzyme F420-dependent N(5),N(10)-methylenetetrahydromethanopterin reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Methylene-H(4)MPT reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4 6" type="primary">mer</name>
        <name evidence="6" type="ordered locus">NP_1056A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the oxidation of methyl-H(4)MPT to methylene-H(4)MPT.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>5-methyl-5,6,7,8-tetrahydromethanopterin + oxidized coenzyme F420-(gamma-L-Glu)(n) + H(+) = 5,10-methylenetetrahydromethanopterin + reduced coenzyme F420-(gamma-L-Glu)(n)</text>
            <dbReference type="Rhea" id="RHEA:21144"/>
            <dbReference type="Rhea" id="RHEA-COMP:12939"/>
            <dbReference type="Rhea" id="RHEA-COMP:14378"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57818"/>
            <dbReference type="ChEBI" id="CHEBI:58116"/>
            <dbReference type="ChEBI" id="CHEBI:133980"/>
            <dbReference type="ChEBI" id="CHEBI:139511"/>
            <dbReference type="EC" id="1.5.98.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the mer family.</text>
    </comment>
    <dbReference type="EC" id="1.5.98.2" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322254.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUG2"/>
    <dbReference type="STRING" id="348780.NP_1056A"/>
    <dbReference type="EnsemblBacteria" id="CAI48619">
        <property type="protein sequence ID" value="CAI48619"/>
        <property type="gene ID" value="NP_1056A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701041"/>
    <dbReference type="KEGG" id="nph:NP_1056A"/>
    <dbReference type="eggNOG" id="arCOG02410">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027853_5_3_2"/>
    <dbReference type="OrthoDB" id="213164at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018537">
        <property type="term" value="F:coenzyme F420-dependent N5,N10-methenyltetrahydromethanopterin reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01097">
        <property type="entry name" value="Tetrahydromethanopterin_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="entry name" value="Luciferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01091">
        <property type="entry name" value="F420_mer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050564">
        <property type="entry name" value="F420-G6PD/mer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019946">
        <property type="entry name" value="MeH4methanopterin_reductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002619">
        <property type="entry name" value="PRK02271.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43244">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43244:SF1">
        <property type="entry name" value="5,10-METHYLENETETRAHYDROMETHANOPTERIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="Bacterial luciferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="3 4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Luciferase-like" evidence="5">
        <location>
            <begin position="9"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI48619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="329" mass="34829" checksum="123862719FA9BE00" modified="2017-05-10" version="1">MIGIELTPEHPVSRIAELGEQAEAEGFDTVFASCHYNNRDPFVALDRIARRTDTVRLGPGVANPYETHPVTLASRVATLDEASDGRAVLGVGAGDASTLENLGFERERPLRRVLETFKVAQDLYAGERVDHDGTFRAADAGLNYADTVGEVPAYVGAQGPHMIRMAAKHADGVLLNGSHPRDFEWAADQVDAGLEDRPDERGSFDFAAYASVSVADDAEAARAAARPPVAFIAAGAAPPVLDRHDIDHAVAEQIGDHISAGAFDEAFDAVTPAMLEAFCIAGTPETVGEQIDDVLEYADSFVAGSPLGPDLDAAVRLAGSALQSRIDRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUG5</accession>
    <name>A0A1U7EUG5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 10">A-type ATP synthase subunit I</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">atpI</name>
        <name evidence="12" type="ordered locus">NP_1020A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322236.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUG5"/>
    <dbReference type="STRING" id="348780.NP_1020A"/>
    <dbReference type="EnsemblBacteria" id="CAI48601">
        <property type="protein sequence ID" value="CAI48601"/>
        <property type="gene ID" value="NP_1020A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702545"/>
    <dbReference type="KEGG" id="nph:NP_1020A"/>
    <dbReference type="eggNOG" id="arCOG04138">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025558_2_1_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016471">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1460.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629:SF63">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="VACUOLAR PROTON ATPASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="378"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="420"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="469"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="502"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="570"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="607"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="674"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="87"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="218"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00068671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI48601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="740" mass="80384" checksum="181C4933C1726070" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUH3</accession>
    <name>A0A1U7EUH3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Probable ribosome biogenesis protein Dim2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">dim2</name>
        <name evidence="4" type="ordered locus">NP_1006A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322229.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUH3"/>
    <dbReference type="STRING" id="348780.NP_1006A"/>
    <dbReference type="EnsemblBacteria" id="CAI48594">
        <property type="protein sequence ID" value="CAI48594"/>
        <property type="gene ID" value="NP_1006A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701030"/>
    <dbReference type="KEGG" id="nph:NP_1006A"/>
    <dbReference type="eggNOG" id="arCOG04150">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064992_3_0_2"/>
    <dbReference type="OrthoDB" id="7870at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000076">
        <property type="entry name" value="KH domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019964">
        <property type="entry name" value="KH_domain_protein_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055211">
        <property type="entry name" value="KH_PNO1_2nd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03665">
        <property type="entry name" value="arCOG04150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010329">
        <property type="entry name" value="PRK13763.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12826">
        <property type="entry name" value="RIBONUCLEASE Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12826:SF13">
        <property type="entry name" value="RNA-BINDING PROTEIN PNO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22891">
        <property type="entry name" value="KH_PNO1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="82"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="187" mass="20455" checksum="30AB07D08736F489" modified="2017-05-10" version="1">MKHVTIPEDRIGVLIGEGGETMRKIESEAEVRLDIDSETGSVRVESVGDPILGLKGPDIVKAIGRGFAPEEALRLLEDDMQLFEIVDIDAATRNKKDLRRKKGRLIGESGRTRELMAELSGADVVIYGSTLGIIGAPEQVDAVRSAAEMILDGAPHGAVYSFLERRHNEMQSNDIEYHQFPDGSRSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUI5</accession>
    <name>A0A1U7EUI5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DUF3209 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1106A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322280.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUI5"/>
    <dbReference type="STRING" id="348780.NP_1106A"/>
    <dbReference type="EnsemblBacteria" id="CAI48644">
        <property type="protein sequence ID" value="CAI48644"/>
        <property type="gene ID" value="NP_1106A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701054"/>
    <dbReference type="KEGG" id="nph:NP_1106A"/>
    <dbReference type="eggNOG" id="arCOG04752">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_162430_0_0_2"/>
    <dbReference type="OrthoDB" id="192815at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021577">
        <property type="entry name" value="DUF3209"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11483">
        <property type="entry name" value="DUF3209"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="123" mass="13518" checksum="985E509A3A3E6378" modified="2017-05-10" version="1">MSCHEIEALRLGLMNSLGIGDDATRRHARDELGDDPEPHIAALADAESLAACRRHLDTALVELEAEIAEADPEAPDYDYLRGRLVAVRDAENALERLLSHGDAFLDDLGESHHTLHEAFPVDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUK6</accession>
    <name>A0A1U7EUK6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cobalt-factor-III C17-methyltransferase</fullName>
            <ecNumber evidence="8">2.1.1.272</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cbiH2</name>
        <name evidence="8" type="synonym">cobJ2</name>
        <name evidence="8" type="ordered locus">NP_1114A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.272" evidence="8"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322284.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUK6"/>
    <dbReference type="STRING" id="348780.NP_1114A"/>
    <dbReference type="EnsemblBacteria" id="CAI48648">
        <property type="protein sequence ID" value="CAI48648"/>
        <property type="gene ID" value="NP_1114A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702573"/>
    <dbReference type="KEGG" id="nph:NP_1114A"/>
    <dbReference type="eggNOG" id="arCOG00647">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047948_2_0_2"/>
    <dbReference type="OrthoDB" id="35891at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11646">
        <property type="entry name" value="Precorrin_3B_C17_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006363">
        <property type="entry name" value="Cbl_synth_CobJ/CibH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051810">
        <property type="entry name" value="Precorrin_MeTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01466">
        <property type="entry name" value="cobJ_cbiH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47036">
        <property type="entry name" value="COBALT-FACTOR III C(17)-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47036:SF1">
        <property type="entry name" value="COBALT-FACTOR III C(17)-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="3 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="7">
        <location>
            <begin position="84"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI48648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="337" mass="35527" checksum="1E515776037D186B" modified="2017-05-10" version="1">MSTDDTTETTDASTTESKCGASEAEASTESESKCGASKTDTTTETESACGADSGSESDSTCGGSDSEEDEVGATVDDFDAEPGRLVAVGLGPGKPKGMTARAKAALADAEHIVGYTTYVELLPDDIVEAADDIHSTPMCGEVSRTEEAIDRALAGNDVAIIGSGDPNVYALAGLALEILESKGGTASAVDFEVVPGVPAAQSCGARLGAPLVNDTVSVSLSDHLTPMEEIESRLHAVAPEGFTIAIYNPWSRKRRENFQTCCEILLEHRDPETPVGIVHGASRDDEKTLITDLGTLPELGEEDIVDMTTTIIVGNEETYVWDDRMVTPRGYETKYDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUQ6</accession>
    <name>A0A1U7EUQ6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">tp33</name>
        <name evidence="11" type="ordered locus">NP_1270A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Potential transporter for phosphate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Membrane</location>
            <topology evidence="2 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322361.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUQ6"/>
    <dbReference type="STRING" id="348780.NP_1270A"/>
    <dbReference type="EnsemblBacteria" id="CAI48726">
        <property type="protein sequence ID" value="CAI48726"/>
        <property type="gene ID" value="NP_1270A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703309"/>
    <dbReference type="KEGG" id="nph:NP_1270A"/>
    <dbReference type="eggNOG" id="arCOG02267">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015355_0_0_2"/>
    <dbReference type="OrthoDB" id="101311at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001204">
        <property type="entry name" value="Phos_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101">
        <property type="entry name" value="PHOSPHATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101:SF80">
        <property type="entry name" value="PHOSPHATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01384">
        <property type="entry name" value="PHO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="179"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="254"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="358"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="320"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI48726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="383" mass="39052" checksum="0FFCF67515C3F0F1" modified="2017-05-10" version="1">MELLLLVGILVAVFVGYNIGGATTAPAFGPAVGAGVLSKVAAAALMTVFFLAGGWTLGREVVDTLGEGIVPGELFTLEASIVVLFFIGFALFISNSFGVPASTSMTAVGSIAGLGLATGTLNWVTMGQIVSWWIVAPIIAFWISGVVGRYFYPTINEWVAIPDSEGNPLRGEGSRRERVGALVVIGIGCLMAFSSGASNVANAIAPLVGSGELSMNLGIIIATVAVGIGAFTIARKTLDTMGNDLTELPLTAAIVVAVISSTATAALAAIGIPASFVIIATMSIVGLGWGRATRTTRARDAVRGEETNVSVGALAADEAATVGDTGPESEQSPPIGEEDPGDIPAASDLFDPKTTGRVILMQNVVPAIATLGAFLVFRFVPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUX4</accession>
    <name>A0A1U7EUX4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1370A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322411.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUX4"/>
    <dbReference type="STRING" id="348780.NP_1370A"/>
    <dbReference type="EnsemblBacteria" id="CAI48776">
        <property type="protein sequence ID" value="CAI48776"/>
        <property type="gene ID" value="NP_1370A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701129"/>
    <dbReference type="KEGG" id="nph:NP_1370A"/>
    <dbReference type="eggNOG" id="arCOG04699">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127463_0_0_2"/>
    <dbReference type="OrthoDB" id="300179at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043830">
        <property type="entry name" value="DUF5807"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19123">
        <property type="entry name" value="DUF5807"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="147" mass="15735" checksum="8C5275B36AADE88B" modified="2017-05-10" version="1">MSGESTVEERYEAFLRGERPDDILVYLHEEGVGSMESLLDIGVRVDDGVVLVLPGDEGRAAFQQATGLDAMDFAGMAMQTDGAIDDDCTGGTCPDADTDDDHYVKFVFAFVEEQNEGVGGIYADGDVAHGYAACACGTTYSEKWVLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUZ1</accession>
    <name>A0A1U7EUZ1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit C</fullName>
            <shortName evidence="1">Asp/Glu-ADT subunit C</shortName>
            <ecNumber evidence="1">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1 3" type="primary">gatC</name>
        <name evidence="3" type="synonym">aatC</name>
        <name evidence="3" type="ordered locus">NP_1454A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-aspartyl-tRNA(Asn) + L-glutamine + ATP + H2O = L-asparaginyl-tRNA(Asn) + L-glutamate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14513"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="Rhea" id="RHEA-COMP:9677"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GatC family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322453.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUZ1"/>
    <dbReference type="STRING" id="348780.NP_1454A"/>
    <dbReference type="EnsemblBacteria" id="CAI48818">
        <property type="protein sequence ID" value="CAI48818"/>
        <property type="gene ID" value="NP_1454A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702361"/>
    <dbReference type="KEGG" id="nph:NP_1454A"/>
    <dbReference type="eggNOG" id="arCOG02726">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105899_1_1_2"/>
    <dbReference type="OrthoDB" id="15210at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050566">
        <property type="term" value="F:asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070681">
        <property type="term" value="P:glutaminyl-tRNAGln biosynthesis via transamidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.60">
        <property type="entry name" value="Glu-tRNAGln amidotransferase C subunit, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00122">
        <property type="entry name" value="GatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036113">
        <property type="entry name" value="Asp/Glu-ADT_sf_sub_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003837">
        <property type="entry name" value="GatC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00135">
        <property type="entry name" value="gatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15004">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT C, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15004:SF0">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT C, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02686">
        <property type="entry name" value="GatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141000">
        <property type="entry name" value="Glu-tRNAGln amidotransferase C subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="89" mass="9982" checksum="43DACE5AA545EA61" modified="2017-05-10" version="1">MTVDDDAVRHVASLARVDLDEDEVELFAEQFGDILEYFEALDDVPDVEPETELANVMRPDEVHEGLTQDEALQNAPETEDGYFKGPKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV27</accession>
    <name>A0A1U7EV27_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Probable bifunctional folylpolyglutamate synthase/dihydropteroate synthase</fullName>
            <ecNumber evidence="6">2.5.1.15</ecNumber>
            <ecNumber evidence="7">6.3.2.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">folCP</name>
        <name evidence="21" type="ordered locus">NP_1478A</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Can complement an H.volcanii mutant strain that is thymidine auxotroph because it lacks the two dihydrofolate reductase genes encoded by hdrA and hdrB.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n) + L-glutamate + ATP = (6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n+1) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10580"/>
            <dbReference type="Rhea" id="RHEA-COMP:14738"/>
            <dbReference type="Rhea" id="RHEA-COMP:14740"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:141005"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(7,8-dihydropterin-6-yl)methyl diphosphate + 4-aminobenzoate = 7,8-dihydropteroate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19949"/>
            <dbReference type="ChEBI" id="CHEBI:17836"/>
            <dbReference type="ChEBI" id="CHEBI:17839"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:72950"/>
            <dbReference type="EC" id="2.5.1.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate: step 1/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; tetrahydrofolylpolyglutamate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the C-terminal section; belongs to the DHPS family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="18">In the N-terminal section; belongs to the folylpolyglutamate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.15" evidence="6"/>
    <dbReference type="EC" id="6.3.2.17" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322465.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV27"/>
    <dbReference type="STRING" id="348780.NP_1478A"/>
    <dbReference type="EnsemblBacteria" id="CAI48830">
        <property type="protein sequence ID" value="CAI48830"/>
        <property type="gene ID" value="NP_1478A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702723"/>
    <dbReference type="KEGG" id="nph:NP_1478A"/>
    <dbReference type="eggNOG" id="arCOG02817">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017222_0_0_2"/>
    <dbReference type="OrthoDB" id="75177at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004156">
        <property type="term" value="F:dihydropteroate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004326">
        <property type="term" value="F:tetrahydrofolylpolyglutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00739">
        <property type="entry name" value="DHPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.10:FF:000011">
        <property type="entry name" value="Folylpolyglutamate synthase/dihydrofolate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="entry name" value="Dihydropteroate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="entry name" value="Mur ligase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="entry name" value="Mur-like, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045031">
        <property type="entry name" value="DHP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006390">
        <property type="entry name" value="DHP_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001645">
        <property type="entry name" value="Folylpolyglutamate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000489">
        <property type="entry name" value="Pterin-binding_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01496">
        <property type="entry name" value="DHPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01499">
        <property type="entry name" value="folC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20941">
        <property type="entry name" value="FOLATE SYNTHESIS PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20941:SF1">
        <property type="entry name" value="FOLIC ACID SYNTHESIS PROTEIN FOL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00809">
        <property type="entry name" value="Pterin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="Dihydropteroate synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="MurD-like peptide ligases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="MurD-like peptide ligases, peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00792">
        <property type="entry name" value="DHPS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00793">
        <property type="entry name" value="DHPS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50972">
        <property type="entry name" value="PTERIN_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="8 21" id="KW-0436">Ligase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 21" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pterin-binding" evidence="20">
        <location>
            <begin position="548"/>
            <end position="799"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00057011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00060901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00068433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAI48830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="812" mass="85087" checksum="4B2AA076FCD64AFB" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV29</accession>
    <name>A0A1U7EV29_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MoaD family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1540A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322495.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV29"/>
    <dbReference type="STRING" id="348780.NP_1540A"/>
    <dbReference type="EnsemblBacteria" id="CAI48861">
        <property type="protein sequence ID" value="CAI48861"/>
        <property type="gene ID" value="NP_1540A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701175"/>
    <dbReference type="KEGG" id="nph:NP_1540A"/>
    <dbReference type="eggNOG" id="arCOG00539">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1821072_0_0_2"/>
    <dbReference type="OrthoDB" id="240306at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd17040">
        <property type="entry name" value="Ubl_MoaD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003749">
        <property type="entry name" value="ThiS/MoaD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02597">
        <property type="entry name" value="ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="MoaD/ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="145" mass="15476" checksum="9CB1565A60D6E4D5" modified="2017-05-10" version="1">MDAGSDTSRVALDESAAGDCGGDAGGATTVTVRCTGHVRDTVGTHELEYTFEGRTLRAFLQAFFGDYDVEDLLIAETEAEATTSGWAPSDGDPPGAWAKNPEGEQTRSFARVAINGQFNEHLDGLNTELADGDRVSLLYPFMFCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV38</accession>
    <name>A0A1U7EV38_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HTH domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1528A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322489.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV38"/>
    <dbReference type="STRING" id="348780.NP_1528A"/>
    <dbReference type="EnsemblBacteria" id="CAI48855">
        <property type="protein sequence ID" value="CAI48855"/>
        <property type="gene ID" value="NP_1528A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701171"/>
    <dbReference type="KEGG" id="nph:NP_1528A"/>
    <dbReference type="eggNOG" id="arCOG01345">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_152992_0_0_2"/>
    <dbReference type="OrthoDB" id="229881at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="101" mass="11692" checksum="B0E2E57FE93B8752" modified="2017-05-10" version="1">MIDRLEKEVDMLERHLRVLRMVIENEPIGIVKMSNETGYPHHKVRYSLRVLEEENLIEPSSQGAITTEDTIEFVEDLDSKVDDIIDKLEGMKIDDVPEIEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV71</accession>
    <name>A0A1U7EV71_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">NP_1622A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322536.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV71"/>
    <dbReference type="STRING" id="348780.NP_1622A"/>
    <dbReference type="EnsemblBacteria" id="CAI48902">
        <property type="protein sequence ID" value="CAI48902"/>
        <property type="gene ID" value="NP_1622A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701203"/>
    <dbReference type="KEGG" id="nph:NP_1622A"/>
    <dbReference type="eggNOG" id="arCOG02360">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010703_0_0_2"/>
    <dbReference type="OrthoDB" id="8127at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004673">
        <property type="term" value="F:protein histidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00075">
        <property type="entry name" value="HATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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            <end position="364"/>
        </location>
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        </location>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI48902.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="884" mass="97886" checksum="A8C41A88898A501C" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVB1</accession>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
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            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI48963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
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</entry>
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        <name evidence="7" type="ordered locus">NP_1840A</name>
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    <organism evidence="7 8">
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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            <authorList>
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
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            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
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        <source>
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        </source>
    </reference>
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        </subcellularLocation>
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    </dbReference>
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        <property type="entry name" value="MamF_MmsF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
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    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
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        <location>
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            <end position="99"/>
        </location>
    </feature>
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        <location>
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            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
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        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
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        <location>
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            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="274"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="21"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="298" mass="31662" checksum="51E0DEDF01ECA8D1" modified="2017-05-10" version="1">MSNTHDSPTDPQPNSAGGSQPTPPTDPAEQTPPGPDILAERTLSGIFVHLLGLLTFFIGPLVVYAAADHDFTRENARNAANWQLFYLAAIVGVFGMLGVTFAADALLPDPVVFVLLLAVFVGTFGIGIGILLNIGFVLVASGKAIFGHAWTYPIAPSFFDRREAVGGDNIPWWTLLSVYAVATPPLFATLAWEAFADGPDADPLFWGVFALLAVVFVLSFVTFALLYKDIEAVRGAEHGWMPDPRLYLGAPVASGIATYPVAAVAGSISPAGDAVYGYMAVLWVAAVVYLVRRVSHGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVJ0</accession>
    <name>A0A1U7EVJ0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1898A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVJ0"/>
    <dbReference type="STRING" id="348780.NP_1898A"/>
    <dbReference type="EnsemblBacteria" id="CAI49040">
        <property type="protein sequence ID" value="CAI49040"/>
        <property type="gene ID" value="NP_1898A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701297"/>
    <dbReference type="KEGG" id="nph:NP_1898A"/>
    <dbReference type="eggNOG" id="arCOG00498">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048478_0_2_2"/>
    <dbReference type="OrthoDB" id="205181at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050855">
        <property type="entry name" value="NDM-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42951:SF4">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE MBLAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42951">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="23"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="320" mass="34445" checksum="3032246CD760C75D" modified="2017-05-10" version="1">MDARSTQGPIHRLAFDVDWRPGHVACYLVDGPEPVLVDAALPEHEAAFRQALAEHDYTPADIDHLLITHPHVDHVGLTPTLLQAGDPTVYAPAGVRERFNRGVAGLRERVEANCREAGFPDEQRSTAVEMAVESLERDSTLLAPEAVDVWLDAGEEANVGGLETTPVHVPGHQADHLCYPVDIDGEDALLAGDMGIKPFRPVVMHDGLDDGYREAFPAFYTALDRLDDIDVERVYPGHGPVHGDLAGAVAHARASLDSRLSQVADLVADGHATAPAVAMSLAGDREIEYLIPEAMSALAHLEAAGEIDSTLNDGVRYYDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVJ8</accession>
    <name>A0A1U7EVJ8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Glycosyl/glycerophosphate transferase (Homolog to polyglycerol phosphate polymerase)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">NP_1920A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDP-glycerol glycerophosphotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322683.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVJ8"/>
    <dbReference type="STRING" id="348780.NP_1920A"/>
    <dbReference type="EnsemblBacteria" id="CAI49051">
        <property type="protein sequence ID" value="CAI49051"/>
        <property type="gene ID" value="NP_1920A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701304"/>
    <dbReference type="KEGG" id="nph:NP_1920A"/>
    <dbReference type="eggNOG" id="arCOG04827">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029598_2_0_2"/>
    <dbReference type="OrthoDB" id="46222at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047355">
        <property type="term" value="F:CDP-glycerol glycerophosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007554">
        <property type="entry name" value="Glycerophosphate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043148">
        <property type="entry name" value="TagF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043149">
        <property type="entry name" value="TagF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051612">
        <property type="entry name" value="Teichoic_Acid_Biosynth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37316">
        <property type="entry name" value="TEICHOIC ACID GLYCEROL-PHOSPHATE PRIMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37316:SF3">
        <property type="entry name" value="TEICHOIC ACID GLYCEROL-PHOSPHATE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04464">
        <property type="entry name" value="Glyphos_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0777">Teichoic acid biosynthesis</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="415" mass="47119" checksum="FC5C1FC187AFFD55" modified="2017-05-10" version="1">MILFSALTIAIVSVASTIASVRNAFAPIRKVLALLATPIVFLVSYLVPRDDSLWLFMAGQGDRFADNSKYLFLYCDCQPDVRNVWIGTDERIVAELREHGYEAYTRHSLAGRWRLLRAGYWFETHGPIAPAYAGRARLIHLTHGNYLKVMLEDHTRDWPWIVQVLVEFFFERRRRYVVTGSGPPLKNMQSMRGAPADRALMTGLPRNDALFDAFQDEELGLEESALAAVRDRAAEGPVLLYAPTYREGYGERNGVPLSELDLGLERLDSVLRSHDATLYISHHPATTFDRDLEGLDRVTVLESGGDLYPFLRECDVLVTDYSGIFYDFLLLDRPMVFFAPDLEVYLEDRDLYFDYEDHVPGTIATTPEAFVESVRAILEGADEHGDDRAAVREAFYDDPDGEACERVYHTVRDEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVK9</accession>
    <name>A0A1U7EVK9_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thymidylate kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1932A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322689.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVK9"/>
    <dbReference type="STRING" id="348780.NP_1932A"/>
    <dbReference type="EnsemblBacteria" id="CAI49057">
        <property type="protein sequence ID" value="CAI49057"/>
        <property type="gene ID" value="NP_1932A"/>
    </dbReference>
    <dbReference type="GeneID" id="32154998"/>
    <dbReference type="KEGG" id="nph:NP_1932A"/>
    <dbReference type="eggNOG" id="arCOG01891">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1113896_0_0_2"/>
    <dbReference type="OrthoDB" id="195711at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="243" mass="27995" checksum="E85F40F8621BE4FC" modified="2017-05-10" version="1">MSHIEFVGPPGAGKSTIYGAVIDNPKFFGALQEDAVKRWMLRKARKRYRILYRMTPGFLRSLLVDSVIEYRLRRAVFDDFVRSYPNFPHVLSLILNEARYQPETLHQLVKRCAETYQIGVETVEDDETLFIDEGFSQSAVSILWRFDDRQFPLSDYFQRTPTPRLLIYVDAPVDTCIQRQQKRGNVPASKEWVSEDIQTVQYDLDNICQQVVTAAEKHTQVLTINNTGGIDTAVDRIRSALSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVW4</accession>
    <name>A0A1U7EVW4_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidyl-prolyl cis-trans isomerase</fullName>
            <ecNumber evidence="9">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">mtfK1</name>
        <name evidence="12" type="ordered locus">NP_2228A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8 9">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the FKBP-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="9"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49205.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_083761667.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVW4"/>
    <dbReference type="STRING" id="348780.NP_2228A"/>
    <dbReference type="EnsemblBacteria" id="CAI49205">
        <property type="protein sequence ID" value="CAI49205"/>
        <property type="gene ID" value="NP_2228A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702947"/>
    <dbReference type="KEGG" id="nph:NP_2228A"/>
    <dbReference type="eggNOG" id="arCOG00981">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098197_2_0_2"/>
    <dbReference type="OrthoDB" id="8615at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47861">
        <property type="entry name" value="FKBP-TYPE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE SLYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47861:SF3">
        <property type="entry name" value="FKBP-TYPE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE SLYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 9" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="11">
        <location>
            <begin position="6"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI49205.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="156" mass="16959" checksum="6D79868F1BB8C2B2" modified="2017-05-10" version="1">MTIATGDAVTLEYTGRLDDGTVFDTSREDVAEEEGLADAQPDREYSPLTVDVGAQEVIEGMEEGLIGLEAGAETTLEIPPEKGYGEWSEERVQTFDTEELQEMLGGQLPEEGAYLEAQNGQHGEVTDVSDDVVRVDFNPELAGETLTFDVEIVDVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVX5</accession>
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        <submittedName>
            <fullName evidence="2">MaoC domain protein</fullName>
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        <name evidence="2" type="primary">maoC3</name>
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        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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            <taxon>Natronomonas</taxon>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVX5"/>
    <dbReference type="STRING" id="348780.NP_2256A"/>
    <dbReference type="EnsemblBacteria" id="CAI49219">
        <property type="protein sequence ID" value="CAI49219"/>
        <property type="gene ID" value="NP_2256A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702688"/>
    <dbReference type="KEGG" id="nph:NP_2256A"/>
    <dbReference type="eggNOG" id="arCOG00776">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094876_1_0_2"/>
    <dbReference type="OrthoDB" id="225748at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd03454">
        <property type="entry name" value="YdeM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002539">
        <property type="entry name" value="MaoC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052342">
        <property type="entry name" value="MCH/BMMD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43664:SF1">
        <property type="entry name" value="BETA-METHYLMALYL-COA DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43664">
        <property type="entry name" value="MONOAMINE OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01575">
        <property type="entry name" value="MaoC_dehydratas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MaoC-like" evidence="1">
        <location>
            <begin position="10"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="151" mass="17441" checksum="0D9DD8DE3F71FF15" modified="2017-05-10" version="1">MARYFEDVEVGEMYELDGRYEITEDEITEFAEKYDPQPFHLDQEAAEESIFGSLAASGWHTASACMRLFVDEFLDAETSMGARGIDNLRWKQPVYPGDEIRIEVEIVDKRPSESRPGMGHIKTRLRGYNQDDEEVIEWTALGMTRRRNPDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVX9</accession>
    <name>A0A1U7EVX9_NATPD</name>
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        <submittedName>
            <fullName evidence="7">M50 family metalloprotease</fullName>
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        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">NP_2268A</name>
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    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
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            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVX9"/>
    <dbReference type="STRING" id="348780.NP_2268A"/>
    <dbReference type="EnsemblBacteria" id="CAI49225">
        <property type="protein sequence ID" value="CAI49225"/>
        <property type="gene ID" value="NP_2268A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701397"/>
    <dbReference type="KEGG" id="nph:NP_2268A"/>
    <dbReference type="eggNOG" id="arCOG02834">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="15212at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:endomembrane system"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031293">
        <property type="term" value="P:membrane protein intracellular domain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="S2P-M50_PDZ_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="MBTPS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008915">
        <property type="entry name" value="Peptidase_M50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13325:SF3">
        <property type="entry name" value="MEMBRANE-BOUND TRANSCRIPTION FACTOR SITE-2 PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13325">
        <property type="entry name" value="PROTEASE M50 MEMBRANE-BOUND TRANSCRIPTION FACTOR SITE 2 PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02163">
        <property type="entry name" value="Peptidase_M50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="65"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="191"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="506"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="563"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="6">
        <location>
            <begin position="209"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="591" mass="61842" checksum="9B08E3E70A2CF965" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW21</accession>
    <name>A0A1U7EW21_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ordered locus">NP_2340A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW21"/>
    <dbReference type="EnsemblBacteria" id="CAI49261">
        <property type="protein sequence ID" value="CAI49261"/>
        <property type="gene ID" value="NP_2340A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_2340A"/>
    <dbReference type="HOGENOM" id="CLU_3362600_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="35" mass="3540" checksum="4ECCE81546DA17B7" modified="2017-05-10" version="1">MQLLDRHLRAEEMALYLAGAATALAGAALAAGVTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW42</accession>
    <name>A0A1U7EW42_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2</fullName>
            <ecNumber evidence="9">2.5.1.77</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cofH</name>
        <name evidence="9" type="ordered locus">NP_2394A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
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        <property type="gene ID" value="NP_2394A"/>
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    <dbReference type="KEGG" id="nph:NP_2394A"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
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        <property type="entry name" value="CofH/MnqC-like_C"/>
    </dbReference>
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        <property type="entry name" value="F420"/>
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        <property type="entry name" value="FO_synthase_su2"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="FO SYNTHASE (COFH)"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="7_8-didemethyl-8-hydroxy-5-dea"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="F420__menaquinone_cofactor_bio"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
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    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Radical SAM core" evidence="8">
        <location>
            <begin position="81"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="425"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI49288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="447" mass="49168" checksum="9462D6702CACE6B1" modified="2017-05-10" version="1">MDAFEASANVPAELQFERRPVTDQSFENALEKARSGVRLDVDDAIELLTTSSDTEGIDQSRKELVLEAADRRRAEVVGEEVTFVANLNNNVTTACNVGCLFCNFKDAAQRFEADYDGDNAGFTKTPAESREVVADAVDRGIYEVTSVSGLHPALALDEEHREILSAADDGHNYQPPSTYETDPGTYVDQLRAMNVDDVHIHSMTPEEAYHARRGTDWDYETVFRRLSDAGLDSVPGTAAEILVDEVRDAICPGKIGSDEWLEAMTAAAEVGLDTTATIMYGHVENEAHRAIHLDRIRDLQDQTGAITEFVPLSFIHQETPLKERGLVDGGASAHEDELMVAVSRLYLDNIDHIQSSWVKYGDTQGLKMLNCGADDFMGTILSEEITKRAGGDYGEFRSIQEYADMITSIGRVPVERSTDYETRRVVDPDASSVGPRLGPRADGTPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW88</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2502A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="EnsemblBacteria" id="CAI49342">
        <property type="protein sequence ID" value="CAI49342"/>
        <property type="gene ID" value="NP_2502A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701467"/>
    <dbReference type="KEGG" id="nph:NP_2502A"/>
    <dbReference type="eggNOG" id="arCOG06307">
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    </dbReference>
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    <dbReference type="OrthoDB" id="162070at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058277">
        <property type="entry name" value="DUF7971"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25926">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="24"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="128" mass="14627" checksum="7DBD7CB53D39B2E1" modified="2017-05-10" version="1">MDEIQLGVPQPLVESLPDDDGAAAQDMHRAIEGYNARLERLIEAAADEGEAAEAILDLVEHFEDRIERFHAFVPELRAWGQSPIYAIAWRNLYADCIEQLYEHDWLAAQLDRERNARLVEDGIRLSDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW91</accession>
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        <recommendedName>
            <fullName evidence="1">DUF8048 domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="2" type="ordered locus">NP_2496A</name>
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    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49339.1"/>
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    <dbReference type="EnsemblBacteria" id="CAI49339">
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        <property type="gene ID" value="NP_2496A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701465"/>
    <dbReference type="KEGG" id="nph:NP_2496A"/>
    <dbReference type="eggNOG" id="arCOG06309">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149761_0_0_2"/>
    <dbReference type="OrthoDB" id="235313at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058361">
        <property type="entry name" value="DUF8048"/>
    </dbReference>
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        <property type="entry name" value="DUF8048"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8048" evidence="1">
        <location>
            <begin position="4"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="124" mass="13955" checksum="058F3334608864D4" modified="2017-05-10" version="1">MSGDPLDGNALVLAAAKASVSGEQLPKLVDDAQSRLADRRDAYRRRYECVHDDDDRTVFLVEEGHWQTLGDEWGLDDQAWRALRRAHREQLGQLAGDLDRRQEFDAALELREVVVIGTEQSVPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWA8</accession>
    <name>A0A1U7EWA8_NATPD</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">NP_2556A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322994.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWA8"/>
    <dbReference type="EnsemblBacteria" id="CAI49369">
        <property type="protein sequence ID" value="CAI49369"/>
        <property type="gene ID" value="NP_2556A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701485"/>
    <dbReference type="KEGG" id="nph:NP_2556A"/>
    <dbReference type="eggNOG" id="arCOG03915">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2420125_0_0_2"/>
    <dbReference type="OrthoDB" id="266325at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055943">
        <property type="entry name" value="DUF7521"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24365">
        <property type="entry name" value="DUF7521"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="96" mass="10660" checksum="BA95016450D99020" modified="2017-05-10" version="1">MVDLLQAVLISMRLLLFGLTLGLTIISFQAYRKQPTERLQYAFIGFAFLSMGVGVTNLTTQLGVQGGIGRSLQYFQIAETVPFIVGFAMIYASLYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWB4</accession>
    <name>A0A1U7EWB4_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF5658 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2552A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322992.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWB4"/>
    <dbReference type="STRING" id="348780.NP_2552A"/>
    <dbReference type="EnsemblBacteria" id="CAI49367">
        <property type="protein sequence ID" value="CAI49367"/>
        <property type="gene ID" value="NP_2552A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701483"/>
    <dbReference type="KEGG" id="nph:NP_2552A"/>
    <dbReference type="eggNOG" id="arCOG04975">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_166566_0_0_2"/>
    <dbReference type="OrthoDB" id="270892at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043717">
        <property type="entry name" value="DUF5658"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18902">
        <property type="entry name" value="DUF5658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="DUF5658" evidence="2">
        <location>
            <begin position="19"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="108" mass="11410" checksum="53EB3907C5D6F1AB" modified="2017-05-10" version="1">MGGLLPRLAEREREVWIAALLLYGVGDTVTTFWGLSLGGVAEAGPIARPLIDAYGRGALIGIKVVVFPTFYVVWRLLRTPGRVAVPLALAVVGAAVTLWNLVVISGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWC9</accession>
    <name>A0A1U7EWC9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Acyl-CoA synthetase</fullName>
            <ecNumber evidence="6">6.2.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">acs6</name>
        <name evidence="6" type="ordered locus">NP_2626A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323029.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWC9"/>
    <dbReference type="STRING" id="348780.NP_2626A"/>
    <dbReference type="EnsemblBacteria" id="CAI49404">
        <property type="protein sequence ID" value="CAI49404"/>
        <property type="gene ID" value="NP_2626A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702502"/>
    <dbReference type="KEGG" id="nph:NP_2626A"/>
    <dbReference type="eggNOG" id="arCOG00856">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_7_2"/>
    <dbReference type="OrthoDB" id="193284at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016878">
        <property type="term" value="F:acid-thiol ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05936">
        <property type="entry name" value="FC-FACS_FadD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000008">
        <property type="entry name" value="2,3-dihydroxybenzoate-AMP ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050237">
        <property type="entry name" value="ATP-dep_AMP-bd_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43767">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43767:SF1">
        <property type="entry name" value="NONRIBOSOMAL PEPTIDE SYNTHASE PES1 (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="4">
        <location>
            <begin position="9"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="5">
        <location>
            <begin position="438"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="315"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI49404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="523" mass="57592" checksum="C89DF347CED2C1BB" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWE7</accession>
    <name>A0A1U7EWE7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Thioesterase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2648A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 4-hydroxybenzoyl-CoA thioesterase family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323040.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWE7"/>
    <dbReference type="STRING" id="348780.NP_2648A"/>
    <dbReference type="EnsemblBacteria" id="CAI49415">
        <property type="protein sequence ID" value="CAI49415"/>
        <property type="gene ID" value="NP_2648A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701511"/>
    <dbReference type="KEGG" id="nph:NP_2648A"/>
    <dbReference type="eggNOG" id="arCOG01137">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101141_2_3_2"/>
    <dbReference type="OrthoDB" id="56956at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00586">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050563">
        <property type="entry name" value="4-hydroxybenzoyl-CoA_TE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31793">
        <property type="entry name" value="4-HYDROXYBENZOYL-COA THIOESTERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31793:SF27">
        <property type="entry name" value="NOVEL THIOESTERASE SUPERFAMILY DOMAIN AND SAPOSIN A-TYPE DOMAIN CONTAINING PROTEIN (0610012H03RIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13279">
        <property type="entry name" value="4HBT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="134" mass="15260" checksum="F1ACE56CE617F31E" modified="2017-05-10" version="1">MEFRFDTDVEVRYQDIDALGHVNNAVYGTYLEQARVRYFDRVLGVPIEDRSMVLANLDVDFRQPVTIDDRQVTVACGVVELGDSSFRMQYRIAPSADSDPAATAETTLVAWDGDGSRPLPEHWREQFEAFEPEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWJ8</accession>
    <name>A0A1U7EWJ8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">PaaI family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_2756A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioesterase PaaI family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWJ8"/>
    <dbReference type="STRING" id="348780.NP_2756A"/>
    <dbReference type="EnsemblBacteria" id="CAI49469">
        <property type="protein sequence ID" value="CAI49469"/>
        <property type="gene ID" value="NP_2756A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_2756A"/>
    <dbReference type="eggNOG" id="arCOG00777">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089876_7_1_2"/>
    <dbReference type="OrthoDB" id="202321at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03443">
        <property type="entry name" value="PaaI_thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039298">
        <property type="entry name" value="ACOT13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003736">
        <property type="entry name" value="PAAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00369">
        <property type="entry name" value="unchar_dom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21660:SF1">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21660">
        <property type="entry name" value="THIOESTERASE SUPERFAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioesterase" evidence="3">
        <location>
            <begin position="55"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="147" mass="15850" checksum="2BBCD411FAA44C00" modified="2017-05-10" version="1">MSETPSDDDIDSALAASLDEHGLFEWLDLDIEAAEPGRIVFSLPFDEKFANLASGTVHGGVTATIIDTASGFALRLTFDDPAAARLTTTDLNVRYVRPARDDLRVEASVVRAGGTMGYTESTVTTVHEGERKTVATGGTSYRLFRHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWL4</accession>
    <name>A0A1U7EWL4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Homolog to antitoxin VapB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2784A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323108.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWL4"/>
    <dbReference type="STRING" id="348780.NP_2784A"/>
    <dbReference type="EnsemblBacteria" id="CAI49483">
        <property type="protein sequence ID" value="CAI49483"/>
        <property type="gene ID" value="NP_2784A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701557"/>
    <dbReference type="KEGG" id="nph:NP_2784A"/>
    <dbReference type="eggNOG" id="arCOG04501">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_184174_0_0_2"/>
    <dbReference type="OrthoDB" id="67352at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04014">
        <property type="entry name" value="MazE_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89447">
        <property type="entry name" value="AbrB/MazE/MraZ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="2">
        <location>
            <begin position="19"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="83" mass="9087" checksum="3B789E0625FA3916" modified="2017-05-10" version="1">MSSESVDSESKVSGNQANIPAHIRRELDIDDGDKLRWHIEDDGTLRVHVVQQRTGTFSDFAGYGGDEETVVTADHDAWGVGDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWN7</accession>
    <name>A0A1U7EWN7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DUF304 domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_2780A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323106.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWN7"/>
    <dbReference type="STRING" id="348780.NP_2780A"/>
    <dbReference type="EnsemblBacteria" id="CAI49481">
        <property type="protein sequence ID" value="CAI49481"/>
        <property type="gene ID" value="NP_2780A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701555"/>
    <dbReference type="KEGG" id="nph:NP_2780A"/>
    <dbReference type="eggNOG" id="arCOG04619">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024617_0_0_2"/>
    <dbReference type="OrthoDB" id="107421at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014529">
        <property type="entry name" value="UCP026631"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005182">
        <property type="entry name" value="YdbS-like_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34473:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34473">
        <property type="entry name" value="UPF0699 TRANSMEMBRANE PROTEIN YDBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03703">
        <property type="entry name" value="bPH_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF026631">
        <property type="entry name" value="UCP026631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="202"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="335"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="360"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="YdbS-like PH" evidence="3">
        <location>
            <begin position="58"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="YdbS-like PH" evidence="3">
        <location>
            <begin position="232"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="YdbS-like PH" evidence="3">
        <location>
            <begin position="380"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="448"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="498" mass="55248" checksum="27BA72ED8E364043" modified="2017-05-10" version="1">MKLHPFSVPLRAIESVISLAWVLIIAILGSPALGGTSAVGVIVAAWFVIALAYQLIYYQRFEYELTADTLDMASGVISRRRREIPIQRVQNVDISRNVLQRAFGVAQINLETAGGSSTEASLRYVSVEEAQRLQSEIGRLKRGQTQREAERSAEPEQELFSITSKELGLLGVVGIDLRLLSFVTVLLPVVSPSLREQFADPLVGLAVTAPLAAAAIVVVTAGISGILAVMNYYGFRLARQNDELLYERGLLQRFSGAIPLSKVQTLTVSENFIARRLRYASLAVETAGYAPGDRGSESAIPLAKRDRVFNLAQSVEDFTDVEFTRPPKRARERYAGRYSIIALIAVIVAFGADAYTDLTFAWYATLALFPLAAVAAHLKWLNLGYDVQSEYIVLREGFWTRTITVVPYYRVQTVLDSQTVFQRRRRLATLVVDTAGSSGLTNRQPRALDIDEERAAELREEVADKLQEMVAYRREERREERVESMTAELADADPNPGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWZ4</accession>
    <name>A0A1U7EWZ4_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tat pathway signal protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3030A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323229.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWZ4"/>
    <dbReference type="STRING" id="348780.NP_3030A"/>
    <dbReference type="EnsemblBacteria" id="CAI49606">
        <property type="protein sequence ID" value="CAI49606"/>
        <property type="gene ID" value="NP_3030A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701633"/>
    <dbReference type="KEGG" id="nph:NP_3030A"/>
    <dbReference type="eggNOG" id="arCOG04512">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_641945_0_0_2"/>
    <dbReference type="OrthoDB" id="212084at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055828">
        <property type="entry name" value="DUF7405"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24152">
        <property type="entry name" value="DUF7405"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="427" mass="46330" checksum="D7A6FB8BEA7F20AE" modified="2017-05-10" version="1">MKFRERDGLSRREYVRAMVAAGGAAGLAACLEAFDDGDTGGNGGDEEPTVPSGDPEARSSRQHAWDETLPTDDDGNLVAPEHHVLLPLSLATDIDDDARTQVETAFQSLERAYEYSPEGLLFAVGYSHAYFDERVDSDPPIPEPTALTSMETPEFDTFDAVIHLASNHPHVVLEAEEALVGDVDAPNGTPMEATLSGVFEPAEPRRTGFLGPGLPTQHTDLDGVPDSIPEDAPFFMGFRSGFTESQAPEDRVTIQDGPFAGGTTMHISSLGLQLRTWFEQDSHFQRVAKMFSPEHAHNESVGEIGERLGTETGVAGDIAEATESHARNQGMVGHAQKAARARDEDGTPPLLRRDFNTVDGDQPGVHFVSLQRTVDEFVEVRDAMTGADLAGDGVGQRLNNGILQYIFVRRRGNFIVPPREDRSLPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWZ6</accession>
    <name>A0A1U7EWZ6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidyl-prolyl cis-trans isomerase</fullName>
            <ecNumber evidence="6">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">mtfK2</name>
        <name evidence="9" type="ordered locus">NP_3090A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5 6">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the FKBP-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323258.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWZ6"/>
    <dbReference type="STRING" id="348780.NP_3090A"/>
    <dbReference type="EnsemblBacteria" id="CAI49636">
        <property type="protein sequence ID" value="CAI49636"/>
        <property type="gene ID" value="NP_3090A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702948"/>
    <dbReference type="KEGG" id="nph:NP_3090A"/>
    <dbReference type="eggNOG" id="arCOG00980">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073526_0_0_2"/>
    <dbReference type="OrthoDB" id="8615at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054016">
        <property type="entry name" value="FKBP26_IF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048261">
        <property type="entry name" value="SlpA/SlyD-like_ins_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47861">
        <property type="entry name" value="FKBP-TYPE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE SLYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47861:SF2">
        <property type="entry name" value="LONG-TYPE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22199">
        <property type="entry name" value="FKBP26_IF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="8">
        <location>
            <begin position="32"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="46"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI49636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="317" mass="35051" checksum="5DB3EC4D9901E0DA" modified="2017-05-10" version="1">MSDESEAVEETDAAESEEADADTQDVDGLQDGDFIRIDYTARTVEEDQLVDTTDEDVAEEEGVADQGEFEPRVIVLGEGHLFPDVEDDIRGKEVGDEGSVVVESEEAFGEYDEEEVRTVSADKIDEDDRYPGARIQIDGQQGVLETIIGGRARVDFNHPLAGEDIEYNYEVLETVEGDLEQAEGLLGMFLDLDLDMWVETDEVEETRVEEPDEDDEDAEPETVTETVEKRTLYVESTPQLSMNQQWMMQKQQIAQQVIDLTDIDRILIQEILDGGGMGMGMGGMMGGMGGAGGEDVDLEEALEEADIDADEIADELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX09</accession>
    <name>A0A1U7EX09_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Taxis cluster protein CheC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cheC2</name>
        <name evidence="5" type="ordered locus">NP_3118A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323272.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX09"/>
    <dbReference type="STRING" id="348780.NP_3118A"/>
    <dbReference type="EnsemblBacteria" id="CAI49650">
        <property type="protein sequence ID" value="CAI49650"/>
        <property type="gene ID" value="NP_3118A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702600"/>
    <dbReference type="KEGG" id="nph:NP_3118A"/>
    <dbReference type="eggNOG" id="arCOG02381">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_654911_0_0_2"/>
    <dbReference type="OrthoDB" id="182374at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17911">
        <property type="entry name" value="CheC_ClassIII"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1550.10">
        <property type="entry name" value="CheC-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007597">
        <property type="entry name" value="CheC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028976">
        <property type="entry name" value="CheC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028051">
        <property type="entry name" value="CheX-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050992">
        <property type="entry name" value="CheZ_family_phosphatases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43693">
        <property type="entry name" value="PROTEIN PHOSPHATASE CHEZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43693:SF1">
        <property type="entry name" value="PROTEIN PHOSPHATASE CHEZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04509">
        <property type="entry name" value="CheC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13690">
        <property type="entry name" value="CheX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103039">
        <property type="entry name" value="CheC-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CheC-like protein" evidence="3">
        <location>
            <begin position="104"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Chemotaxis phosphatase CheX-like" evidence="4">
        <location>
            <begin position="251"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="389" mass="42290" checksum="38066F91CD48623A" modified="2017-05-10" version="1">MRIAINQLEEYNYLASRGAKQAARALSQMTGIKMRHEVTGVSLVSDEALADIFTGQKYVGVQVGLSGGIAGETVLIFDPNSAGRLRKRLQSRGGESTLAGGAFAELGNIMIGGFIDGWADHLGTEVDMTPPTYIEAAGTRILPPITRRDAEEYGVFLFESRLQAVNHDLSVPIYLIPEYSEFVKLLRAQPSGLAVPAEKLTVFNGFADQSARRASQQISQLTGLRTDVTVSQLRFISFSDISSEVGTNEYVSVVFELTGRPSGYFVVMFSEDSASRVASAMLPGGVDPDNGGMMQEAIKELGNIVTSGFIDGWANTLQSSIEHSPPEYIEDSGSNIIETVQRRLSDQQEYAFTIDTTITIDNRQAEVQLYVLPDTRELAEALSALPVQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX15</accession>
    <name>A0A1U7EX15_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transducer protein Htr24</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">htr24</name>
        <name evidence="9" type="ordered locus">NP_3134A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX15"/>
    <dbReference type="STRING" id="348780.NP_3134A"/>
    <dbReference type="EnsemblBacteria" id="CAI49658">
        <property type="protein sequence ID" value="CAI49658"/>
        <property type="gene ID" value="NP_3134A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3134A"/>
    <dbReference type="eggNOG" id="arCOG02320">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_18_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06225">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.950">
        <property type="entry name" value="Methyl-accepting chemotaxis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32089:SF112">
        <property type="entry name" value="LYSOZYME-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32089">
        <property type="entry name" value="METHYL-ACCEPTING CHEMOTAXIS PROTEIN MCPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158472">
        <property type="entry name" value="HAMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58104">
        <property type="entry name" value="Methyl-accepting chemotaxis protein (MCP) signaling domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0807">Transducer</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="56"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="8">
        <location>
            <begin position="81"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="8">
        <location>
            <begin position="154"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="7">
        <location>
            <begin position="226"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="517"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="128"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="227"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="519"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI49658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="550" mass="57785" checksum="A04C270150F5A545" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX62</accession>
    <name>A0A1U7EX62_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CRISPR-associated protein Csc1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">csc1</name>
        <name evidence="2" type="ordered locus">NP_3268A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323346.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX62"/>
    <dbReference type="STRING" id="348780.NP_3268A"/>
    <dbReference type="EnsemblBacteria" id="CAI49725">
        <property type="protein sequence ID" value="CAI49725"/>
        <property type="gene ID" value="NP_3268A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701709"/>
    <dbReference type="KEGG" id="nph:NP_3268A"/>
    <dbReference type="eggNOG" id="arCOG05134">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095955_0_0_2"/>
    <dbReference type="OrthoDB" id="259743at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09711">
        <property type="entry name" value="Csc1_I-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017576">
        <property type="entry name" value="CRISPR-assoc_prot_Csc1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03159">
        <property type="entry name" value="cas_Csc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26241">
        <property type="entry name" value="Cas_Csc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="247" mass="27488" checksum="EC8084BB365FD5FC" modified="2017-05-10" version="1">MEVLEATLTTHGKVGFASREVGRMADTDPYILNTALHYALGLAGGNYVDVTHQPTYIEDTADIVDEVYVTPATPVAVESESIRTEYLTTNRNARADTYATPNYPAEDDPTGKASQNLPTFERERALAPGNHFRFYVFPYGRTAKSAKETLPSYIRLGKKRGKGRVRYRIVEAERKSGTFSLGHPLSIYDHDAQPLGGVVMRQMQPTPLWFEGKFEADHYAIESPTESADRVYYPAGAKFLATKRGDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXE2</accession>
    <name>A0A1U7EXE2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">cytidine deaminase</fullName>
            <ecNumber evidence="4">3.5.4.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Cytidine aminohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">cdd</name>
        <name evidence="14" type="ordered locus">NP_3512A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">This enzyme scavenges exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>cytidine + H2O + H(+) = uridine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16069"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:17562"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.5" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323467.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXE2"/>
    <dbReference type="STRING" id="348780.NP_3512A"/>
    <dbReference type="EnsemblBacteria" id="CAI49847">
        <property type="protein sequence ID" value="CAI49847"/>
        <property type="gene ID" value="NP_3512A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702594"/>
    <dbReference type="KEGG" id="nph:NP_3512A"/>
    <dbReference type="eggNOG" id="arCOG04173">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097262_0_1_2"/>
    <dbReference type="OrthoDB" id="39143at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004126">
        <property type="term" value="F:cytidine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055086">
        <property type="term" value="P:nucleobase-containing small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072527">
        <property type="term" value="P:pyrimidine-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01283">
        <property type="entry name" value="cytidine_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000008">
        <property type="entry name" value="Cytidine deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050202">
        <property type="entry name" value="Cyt/Deoxycyt_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006262">
        <property type="entry name" value="Cyt_deam_tetra"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01354">
        <property type="entry name" value="cyt_deam_tetra"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004064">
        <property type="entry name" value="PRK05578.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11644">
        <property type="entry name" value="CYTIDINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11644:SF2">
        <property type="entry name" value="CYTIDINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="13">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="42"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR606262-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR606262-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR606262-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAI49847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="130" mass="13908" checksum="84DFACC98D41D278" modified="2017-05-10" version="1">MDDADLIDAARKAVEQSYAPYSEYYVGAAIATADGAVFSGCNIENANYSNSVHAEELALSKAVEAGHRSFDRIAVSSARRDGVTPCGMCRQSLAEFCGDDLRIICDGPDGRVEYTLGELLPETITASHLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXG6</accession>
    <name>A0A1U7EXG6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Oxidoreductase (Homolog to thioredoxin-disulfide reductase)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_3586A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXG6"/>
    <dbReference type="STRING" id="348780.NP_3586A"/>
    <dbReference type="EnsemblBacteria" id="CAI49884">
        <property type="protein sequence ID" value="CAI49884"/>
        <property type="gene ID" value="NP_3586A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3586A"/>
    <dbReference type="eggNOG" id="arCOG01301">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068376_1_1_2"/>
    <dbReference type="OrthoDB" id="168847at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-dep_OxRdtase_2_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050097">
        <property type="entry name" value="Ferredoxin-NADP_redctase_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48105">
        <property type="entry name" value="THIOREDOXIN REDUCTASE 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-dependent oxidoreductase 2 FAD-binding" evidence="3">
        <location>
            <begin position="9"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="258" mass="28204" checksum="60D1730E53935BF1" modified="2017-05-10" version="1">MADTSFDYDVVIVGGGPAGLSAGVFTARADFRTLVVEHNRSLLRKSAHLENYLGFPAGVRPTTFLEIAQDHATNAGCVYVSGEVVDIKHAHSEDHSGDAPETGAVDSGFIVSTADQQFETRYVIVASWSDCSFLDTLGVERGTEDGGHVPIIEVDADGRTSVDGVYAAGRITDQHHQAIVNAGHGAHVAIALIEEVNPAFWNDWVAIEGYYDSYDKEVPVGVEEISHEERERRIRDSRATMRRYFGEHSVNEPEDAMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXI6</accession>
    <name>A0A1U7EXI6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Major facilitator superfamily transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">tp22</name>
        <name evidence="8" type="ordered locus">NP_3610A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323516.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXI6"/>
    <dbReference type="STRING" id="348780.NP_3610A"/>
    <dbReference type="EnsemblBacteria" id="CAI49896">
        <property type="protein sequence ID" value="CAI49896"/>
        <property type="gene ID" value="NP_3610A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703298"/>
    <dbReference type="KEGG" id="nph:NP_3610A"/>
    <dbReference type="eggNOG" id="arCOG00130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052485_0_0_2"/>
    <dbReference type="OrthoDB" id="204590at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050189">
        <property type="entry name" value="MFS_Efflux_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43124:SF3">
        <property type="entry name" value="CHLORAMPHENICOL EFFLUX PUMP RV0191"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43124">
        <property type="entry name" value="PURINE EFFLUX PUMP PBUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="42"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="133"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="264"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="288"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="314"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="353"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="381"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="1"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="423" mass="43715" checksum="B03C0767A03ECC34" modified="2017-05-10" version="1">MELASNDRSIAAFTMLGHGVVHWFELAIPILLVVWLDAFDVSVAVIGLVVALGYAPFGLGALPAGVLVDRFGPKQPILWCLTGMSLSFAALAVAPSIYAVALCLLAWGIAASVYHPAGLALISTGVEDRGTVFAWHGIAGNLGIALGPFATATLLLFFEWQLVALALAVPGVVATAYGLSADFDSTAAVDDTPDDAGGSGRSSLVSDSKSLFASSFLLVFSIVTIVGLYYRGVLTYLPEILQGLPAMAGVALPAGIEELSLGDYFYVGLLVAGMAGQYVAGKLTRRMPVARGLVVVFGALAVLAVVFIPVSGLGLVAVLVYCAVLGFTLFAIEPFYQEAVAVYTPADSRGLSYGYTYLGMFGLGALNISFGGFLLEYLSLTAFFLSLSVIAVAGTAVAWRLITAPVGQAAPAAEADAASSDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXK2</accession>
    <name>A0A1U7EXK2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ArsR family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3596A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323509.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXK2"/>
    <dbReference type="STRING" id="348780.NP_3596A"/>
    <dbReference type="EnsemblBacteria" id="CAI49889">
        <property type="protein sequence ID" value="CAI49889"/>
        <property type="gene ID" value="NP_3596A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701818"/>
    <dbReference type="KEGG" id="nph:NP_3596A"/>
    <dbReference type="eggNOG" id="arCOG03065">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124803_4_0_2"/>
    <dbReference type="OrthoDB" id="290446at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="120"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="134" mass="14944" checksum="EC6478258478B9D5" modified="2017-05-10" version="1">MTNDPDDIFEVLSSDVSREILAAASVGPVSAQELENVCDASLPTIYRRLEMLQRYDFISEEQAVDPDGGQYKQYSTDLKEITIVIEDGGYDVDIRVRKDTVDKFGELFRDLREGHRESDASGPSESGSSTEDTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXM6</accession>
    <name>A0A1U7EXM6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein eL24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 8" type="primary">rpl24e</name>
        <name evidence="8" type="ordered locus">NP_3664A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 50S ribosomal subunit. Forms a cluster with proteins L3 and L14.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eL24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323541.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXM6"/>
    <dbReference type="STRING" id="348780.NP_3664A"/>
    <dbReference type="EnsemblBacteria" id="CAI49923">
        <property type="protein sequence ID" value="CAI49923"/>
        <property type="gene ID" value="NP_3664A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703135"/>
    <dbReference type="KEGG" id="nph:NP_3664A"/>
    <dbReference type="eggNOG" id="arCOG01950">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_152994_0_0_2"/>
    <dbReference type="OrthoDB" id="55506at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00472">
        <property type="entry name" value="Ribosomal_L24e_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.20">
        <property type="entry name" value="Ribosomal protein L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00773">
        <property type="entry name" value="Ribosomal_eL24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038630">
        <property type="entry name" value="L24e/L24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055345">
        <property type="entry name" value="Ribosomal_eL24-rel_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000988">
        <property type="entry name" value="Ribosomal_eL24-rel_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023442">
        <property type="entry name" value="Ribosomal_eL24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011017">
        <property type="entry name" value="TRASH_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF034186">
        <property type="entry name" value="PRK14891.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01246">
        <property type="entry name" value="Ribosomal_L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00746">
        <property type="entry name" value="TRASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01073">
        <property type="entry name" value="RIBOSOMAL_L24E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TRASH" evidence="7">
        <location>
            <begin position="7"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="52"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="57"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="107" mass="11809" checksum="ED9D5830397EE5CC" modified="2017-05-10" version="1">MVQKRTCDYSGQEIEPGTGTMYVKNDGTVLWFADSKCEKNYFMGREARDLEWTGGEEAAEDEAADEEDEAAEAADEEAADEAAEETPDLEAAEEEAADEEDEEEVQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXU2</accession>
    <name>A0A1U7EXU2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rad50/SbcC-type AAA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3882A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323649.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXU2"/>
    <dbReference type="STRING" id="348780.NP_3882A"/>
    <dbReference type="EnsemblBacteria" id="CAI50032">
        <property type="protein sequence ID" value="CAI50032"/>
        <property type="gene ID" value="NP_3882A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701901"/>
    <dbReference type="KEGG" id="nph:NP_3882A"/>
    <dbReference type="eggNOG" id="arCOG00369">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_329220_0_0_2"/>
    <dbReference type="OrthoDB" id="25344at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41259">
        <property type="entry name" value="DOUBLE-STRAND BREAK REPAIR RAD50 ATPASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41259:SF1">
        <property type="entry name" value="DOUBLE-STRAND BREAK REPAIR RAD50 ATPASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad50/SbcC-type AAA" evidence="2">
        <location>
            <begin position="5"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="193"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="331"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="386"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="501"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="723"/>
            <end position="764"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="959" mass="109053" checksum="C3627560929AA63C" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXU6</accession>
    <name>A0A1U7EXU6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3858A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323637.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXU6"/>
    <dbReference type="STRING" id="348780.NP_3858A"/>
    <dbReference type="EnsemblBacteria" id="CAI50020">
        <property type="protein sequence ID" value="CAI50020"/>
        <property type="gene ID" value="NP_3858A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701890"/>
    <dbReference type="KEGG" id="nph:NP_3858A"/>
    <dbReference type="eggNOG" id="arCOG03847">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1718286_0_0_2"/>
    <dbReference type="OrthoDB" id="258845at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10770">
        <property type="entry name" value="Hypothetical protein VC1899 like domain (Restriction endonuclease-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="163" mass="17929" checksum="4851B3A9C6075A16" modified="2017-05-10" version="1">MDHVWISSGSTTIEAMVNPIAAACVGDGIDEQFIPDTVYFLNNPGLGDSIDTASNLTRTILEQHGVAEPEIRIGTIDHETEFDAIVSHFRGAIEAAQDADAEIAVDVTPGRKFMSVIAFNAGTTFDVDHIFYFYLKSSKYYSRIYPDIPKPAAELYDFTEVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXV9</accession>
    <name>A0A1U7EXV9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3866A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323641.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXV9"/>
    <dbReference type="STRING" id="348780.NP_3866A"/>
    <dbReference type="EnsemblBacteria" id="CAI50024">
        <property type="protein sequence ID" value="CAI50024"/>
        <property type="gene ID" value="NP_3866A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701893"/>
    <dbReference type="KEGG" id="nph:NP_3866A"/>
    <dbReference type="eggNOG" id="ENOG502N62H">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2712643_0_0_2"/>
    <dbReference type="OrthoDB" id="350197at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="73" mass="8413" checksum="D74711F74989DAE4" modified="2017-05-10" version="1">MTELTDREHEVATNVWGEDTPINLREIDTEQLVAMHDRVSKAVQASYDDNDVESLGILLSLERKILDVLYERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXW0</accession>
    <name>A0A1U7EXW0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="13">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">NP_3868A</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14 20">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 20">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. UvrD subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="13"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323642.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXW0"/>
    <dbReference type="STRING" id="348780.NP_3868A"/>
    <dbReference type="EnsemblBacteria" id="CAI50025">
        <property type="protein sequence ID" value="CAI50025"/>
        <property type="gene ID" value="NP_3868A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701894"/>
    <dbReference type="KEGG" id="nph:NP_3868A"/>
    <dbReference type="eggNOG" id="arCOG00786">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG00798">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_302005_0_0_2"/>
    <dbReference type="OrthoDB" id="203178at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000725">
        <property type="term" value="P:recombinational repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17932">
        <property type="entry name" value="DEXQc_UvrD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.486.10">
        <property type="entry name" value="PCRA, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013986">
        <property type="entry name" value="DExx_box_DNA_helicase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038726">
        <property type="entry name" value="PDDEXK_AddAB-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070:SF2">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE SRS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12705">
        <property type="entry name" value="PDDEXK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00580">
        <property type="entry name" value="UvrD-helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51217">
        <property type="entry name" value="UVRD_HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6 15 18" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 15 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="16">
        <location>
            <begin position="4"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="UvrD-like helicase C-terminal" evidence="17">
        <location>
            <begin position="396"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="25"/>
            <end position="32"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAI50025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="960" mass="109064" checksum="58859BCC8CC1090B" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYI0</accession>
    <name>A0A1U7EYI0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">GufA family transport protein (Probable substrate zinc)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">gufA</name>
        <name evidence="6" type="synonym">tp45</name>
        <name evidence="6" type="ordered locus">NP_4402A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323907.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYI0"/>
    <dbReference type="STRING" id="348780.NP_4402A"/>
    <dbReference type="EnsemblBacteria" id="CAI50292">
        <property type="protein sequence ID" value="CAI50292"/>
        <property type="gene ID" value="NP_4402A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703323"/>
    <dbReference type="KEGG" id="nph:NP_4402A"/>
    <dbReference type="eggNOG" id="arCOG00576">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015114_1_0_2"/>
    <dbReference type="OrthoDB" id="339454at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF70">
        <property type="entry name" value="OS05G0316100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="106"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="137"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI50292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="266" mass="28048" checksum="FECBBFD7193B467D" modified="2017-05-10" version="1">MLLEGLLFVLAAGIFTAIVCGFGTLPFFFVDEISDRVTVALWGLAGGIMLFASLFGFIFEGLRDGTVFEVGAGLVVGVVVVVVADRIITGYEFEPRDIPDADFRKLVLIVGVLTVHSFPEGVALGVAFADLGIEGDLVLAGLAIPGLAIFITAAISIQNIPEGLAVAIPLHTYGIANWKIFGWAVFSSIPQPIGAGIAYVFVTTAREFLPFGFGFASGAMIFLVLHDIFPEGLDHGSDLPGHGRRELLVGIAVGVAIMLPVMLLTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYL2</accession>
    <name>A0A1U7EYL2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Carbamoyl phosphate synthase large chain</fullName>
            <ecNumber evidence="18">6.3.4.16</ecNumber>
            <ecNumber evidence="18">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Carbamoyl phosphate synthetase ammonia chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18 21" type="primary">carB</name>
        <name evidence="21" type="ordered locus">NP_4506A</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16 18">Large subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The large subunit (synthetase) binds the substrates ammonia (free or transferred from glutamine from the small subunit), hydrogencarbonate and ATP and carries out an ATP-coupled ligase reaction, activating hydrogencarbonate by forming carboxy phosphate which reacts with ammonia to form carbamoyl phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate + L-glutamate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 18">
            <text>hydrogencarbonate + NH4(+) + 2 ATP = carbamoyl phosphate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18029"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="18">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="18">Binds 4 Mg(2+) or Mn(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 18">Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 18">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17 18">Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.</text>
    </comment>
    <comment type="domain">
        <text evidence="18">The large subunit is composed of 2 ATP-grasp domains that are involved in binding the 2 ATP molecules needed for carbamoyl phosphate synthesis. The N-terminal ATP-grasp domain (referred to as the carboxyphosphate synthetic component) catalyzes the ATP-dependent phosphorylation of hydrogencarbonate to carboxyphosphate and the subsequent nucleophilic attack by ammonia to form a carbamate intermediate. The C-terminal ATP-grasp domain (referred to as the carbamoyl phosphate synthetic component) then catalyzes the phosphorylation of carbamate with the second ATP to form the end product carbamoyl phosphate. The reactive and unstable enzyme intermediates are sequentially channeled from one active site to the next through the interior of the protein over a distance of at least 96 A.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 18">Belongs to the CarB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.16" evidence="18"/>
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    <dbReference type="EMBL" id="CR936257">
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    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00062056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAI50344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="1077" mass="116890" checksum="EBFB64F2D746632B" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYQ1</accession>
    <name>A0A1U7EYQ1_NATPD</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">NP_4560A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323986.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYQ1"/>
    <dbReference type="EnsemblBacteria" id="CAI50371">
        <property type="protein sequence ID" value="CAI50371"/>
        <property type="gene ID" value="NP_4560A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702097"/>
    <dbReference type="KEGG" id="nph:NP_4560A"/>
    <dbReference type="eggNOG" id="arCOG06368">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1118238_0_0_2"/>
    <dbReference type="OrthoDB" id="156475at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="252" mass="27197" checksum="E6ED4BD9A209C6EE" modified="2017-05-10" version="1">MSDGGLVEGIKVDVVRLHETWMELVFPRQRTAVHEVLGKWQPRTRGDKIKYRLWAALGVPLVALLYPLLLVGFAARFTARRFNSARAHLGLAGVVVLTALLWGGLTALSRLRLAFEGSMAVGAASAVAVLSAALAAIFIKIGGRGTTVIFAYPFAMNAVFLPPVVAALYDPSLSGVLEQSETLAIFLLDNVLNVYGIDEFLRQFSLEGIGVVLMWVGIATPLGWFLGFVVTLADLIRPRSSDTDEESAEEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYQ6</accession>
    <name>A0A1U7EYQ6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4554A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323983.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYQ6"/>
    <dbReference type="STRING" id="348780.NP_4554A"/>
    <dbReference type="EnsemblBacteria" id="CAI50368">
        <property type="protein sequence ID" value="CAI50368"/>
        <property type="gene ID" value="NP_4554A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702096"/>
    <dbReference type="KEGG" id="nph:NP_4554A"/>
    <dbReference type="eggNOG" id="arCOG04667">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1880986_0_0_2"/>
    <dbReference type="OrthoDB" id="337273at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043927">
        <property type="entry name" value="DUF5778"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19090">
        <property type="entry name" value="DUF5778"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="134" mass="14652" checksum="2BCD8870D58E66F9" modified="2017-05-10" version="1">MDSAVEDDLYKRTKALLEPGDIELNGVIVDTDLTNDEEPTLHQATLDVGNVIAAAAGIEPTDTYVYSGNDDEEFGINQHQGLTLDGDEFVWECQQLLREGTYKVVFYYEASADQTAIVEGVEGLGFEATSVRGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYR1</accession>
    <name>A0A1U7EYR1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Acetyl-CoA C-acyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">acaB5</name>
        <name evidence="7" type="ordered locus">NP_4580A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.16" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323996.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYR1"/>
    <dbReference type="STRING" id="348780.NP_4580A"/>
    <dbReference type="EnsemblBacteria" id="CAI50381">
        <property type="protein sequence ID" value="CAI50381"/>
        <property type="gene ID" value="NP_4580A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702463"/>
    <dbReference type="KEGG" id="nph:NP_4580A"/>
    <dbReference type="eggNOG" id="arCOG01282">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031026_2_3_2"/>
    <dbReference type="OrthoDB" id="25212at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003988">
        <property type="term" value="F:acetyl-CoA C-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020610">
        <property type="entry name" value="Thiolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43365:SF1">
        <property type="entry name" value="ACETYL-COA C-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43365">
        <property type="entry name" value="BLR7806 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00099">
        <property type="entry name" value="THIOLASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="5">
        <location>
            <begin position="5"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="6">
        <location>
            <begin position="260"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI50381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="382" mass="40705" checksum="30ADCB53A1781492" modified="2017-05-10" version="1">MNNPVIVDAVRTPFGKRDGALADMHPQDLAAEPLVALRERNGFAPETIEDVIYGCVTPIDEQGLNIARLAPMVAGWGDGVPGVQLNRMCGSGQQAVNFAAGQVASGAHDVLIAGGVEHMTRVPMGSDGQSVTDTYFEHFDELTTQGEGAERIAETYDFSRRTLDELAADSQSRCGDAWEAGHYDEQVVSVDTEVGDERVELTRDEHMRPDTDAETLSSLPLSFRDPGEGVHHAGNASGIVDGASALLVTSEAAAEEHGWEPMARIVDSHVVGVDPVTMLTGPIPATEELLAQHDLTVDDIDRFEVNEAFAAVVEAWLDETGADWDRTNVWGGAIAHGHPLGATGAALLGKLPYQLAACDGQYGLCTMCIGFGQGIATIIERY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYS1</accession>
    <name>A0A1U7EYS1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF7322 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_4602A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYS1"/>
    <dbReference type="STRING" id="348780.NP_4602A"/>
    <dbReference type="EnsemblBacteria" id="CAI50392">
        <property type="protein sequence ID" value="CAI50392"/>
        <property type="gene ID" value="NP_4602A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_4602A"/>
    <dbReference type="eggNOG" id="arCOG06289">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1870658_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055746">
        <property type="entry name" value="DUF7322"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24008">
        <property type="entry name" value="DUF7322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="73"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="105"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7322" evidence="3">
        <location>
            <begin position="66"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="135"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="152" mass="16276" checksum="B991B4D87F98A1E3" modified="2017-05-10" version="1">MGTKAYPETNAFEDVDHTVSVNPLDEDNEGEVPFGEPSSAEQELAPDAPDASVQTFETESDFSSMSEVSSETLGAFGACFVYAHLALFLGAIGPMGWYFEGWSRAGPLLFAGGVVAGWLTYRRYRKWERQRATDDTAGSDTATAAADGEDAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYS6</accession>
    <name>A0A1U7EYS6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Glutamine--fructose-6-phosphate aminotransferase [isomerizing]</fullName>
            <ecNumber evidence="3 11">2.6.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">D-fructose-6-phosphate amidotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">GFAT</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Glucosamine-6-phosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Hexosephosphate aminotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">L-glutamine--D-fructose-6-phosphate amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">glmS1</name>
        <name evidence="11" type="synonym">glmS</name>
        <name evidence="14" type="ordered locus">NP_4654A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 11">Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>D-fructose 6-phosphate + L-glutamine = D-glucosamine 6-phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:13237"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="2.6.1.16"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.6.1.16" evidence="3 11"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324032.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYS6"/>
    <dbReference type="STRING" id="348780.NP_4654A"/>
    <dbReference type="EnsemblBacteria" id="CAI50418">
        <property type="protein sequence ID" value="CAI50418"/>
        <property type="gene ID" value="NP_4654A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702752"/>
    <dbReference type="KEGG" id="nph:NP_4654A"/>
    <dbReference type="eggNOG" id="arCOG00057">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012520_5_2_2"/>
    <dbReference type="OrthoDB" id="372195at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004360">
        <property type="term" value="F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006047">
        <property type="term" value="P:UDP-N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00714">
        <property type="entry name" value="GFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05009">
        <property type="entry name" value="SIS_GlmS_GlmD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000001">
        <property type="entry name" value="Glutamine--fructose-6-phosphate aminotransferase [isomerizing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000006">
        <property type="entry name" value="Glutamine--fructose-6-phosphate aminotransferase [isomerizing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00164">
        <property type="entry name" value="GlmS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005855">
        <property type="entry name" value="GFAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047084">
        <property type="entry name" value="GFAT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035466">
        <property type="entry name" value="GlmS/AgaS_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035490">
        <property type="entry name" value="GlmS/FrlB_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01135">
        <property type="entry name" value="glmS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001484">
        <property type="entry name" value="PRK00331.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937">
        <property type="entry name" value="GLUCOSAMINE--FRUCTOSE-6-PHOSPHATE AMINOTRANSFERASE, ISOMERIZING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937:SF0">
        <property type="entry name" value="GLUTAMINE--FRUCTOSE-6-PHOSPHATE TRANSAMINASE (ISOMERIZING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13522">
        <property type="entry name" value="GATase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11 14" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 11 14" id="KW-0808">Transferase</keyword>
    <feature type="initiator methionine" description="Removed" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="12">
        <location>
            <begin position="2"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="13">
        <location>
            <begin position="280"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="13">
        <location>
            <begin position="453"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; for GATase activity" evidence="11">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="active site" description="For Fru-6P isomerization activity" evidence="11">
        <location>
            <position position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAI50418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="603" mass="64511" checksum="ABDFCE2018EF1F89" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ23</accession>
    <name>A0A1U7EZ23_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ferredoxin (2Fe-2S)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ferA4</name>
        <name evidence="2" type="ordered locus">NP_4918A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324162.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ23"/>
    <dbReference type="STRING" id="348780.NP_4918A"/>
    <dbReference type="EnsemblBacteria" id="CAI50550">
        <property type="protein sequence ID" value="CAI50550"/>
        <property type="gene ID" value="NP_4918A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702704"/>
    <dbReference type="KEGG" id="nph:NP_4918A"/>
    <dbReference type="eggNOG" id="arCOG02987">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082632_10_2_2"/>
    <dbReference type="OrthoDB" id="180223at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="1">
        <location>
            <begin position="2"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="109" mass="11777" checksum="A75B8DD7083BAF81" modified="2017-05-10" version="1">MPTVTAFGREIECEEGAILRDILLNAGLSPHNGRSDLLNCRGLGTCGTCAVEIDGAVSNIGRRERSRLAVPPHDPESGLRLACQTRVLGDVTVTKYPGFWGQHTRCESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ65</accession>
    <name>A0A1U7EZ65_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">rRNA maturation endonuclease Nob1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">nob1</name>
        <name evidence="5" type="ordered locus">NP_4986A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
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            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="4">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI50584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="151" mass="17135" checksum="D4A02C73F2F6519B" modified="2017-05-10" version="1">MHVLDSSAFINDYTTDEQLATIPLVREELESEAGYRFDALEGSGMRIHIPDPETVERVKRAARETGDAETLSRTDIRLLATAFELDGVLVTDDYAMQNVADKLDTAVEVIAQEGIDERRDWNYQCQGCGRTFDEHHERCQICGSDLERKRP</sequence>
</entry>
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            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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    </gene>
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        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
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            <taxon>Halobacteriales</taxon>
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            <taxon>Natronomonas</taxon>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
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                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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        <property type="component" value="Chromosome"/>
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        <property type="entry name" value="DUF8015"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="62" mass="5999" checksum="3B312AD8D034FE7B" modified="2017-05-10" version="1">MPDTPTRYDGLLAAMPASMAGGAAAGWWSTAPLAAGLGLGSAVAAAFVAVSLFVVPPVPSRG</sequence>
</entry>
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    <accession>A0A1U7EZD0</accession>
    <name>A0A1U7EZD0_NATPD</name>
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        <submittedName>
            <fullName evidence="5">Histidine triad family protein (Homolog to bis(5'-nucleosyl)-tetraphosphatase)</fullName>
        </submittedName>
    </protein>
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        <name evidence="5" type="primary">hit2</name>
        <name evidence="5" type="ordered locus">NP_5090A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
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            <taxon>Natronomonas</taxon>
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    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAI50636.1"/>
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        <property type="gene ID" value="NP_5090A"/>
    </dbReference>
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    <dbReference type="KEGG" id="nph:NP_5090A"/>
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
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        <property type="entry name" value="Histidine_triad_HIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46648:SF1">
        <property type="entry name" value="ADENOSINE 5'-MONOPHOSPHORAMIDASE HNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46648">
        <property type="entry name" value="HIT FAMILY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00332">
        <property type="entry name" value="HISTRIAD"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="6"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="2 3">
        <location>
            <begin position="98"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="1">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR601310-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR601310-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI50636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="142" mass="15174" checksum="4BC107FB7F68A936" modified="2017-05-10" version="1">MSDRTIFEQIIDGDIPARIVHETETTLAFLDANPLAPGHTLVVPKEPYERLRDLPPELSADVFETVRTVTPAVQDAVDADATTIGINDGPEAGQEVPHLHVHIVPRFEGDGGGPIHGVAGKTPELGDEELDTIADDIAEGLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZD5</accession>
    <name>A0A1U7EZD5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5180A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324291.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A1U7EZD5"/>
    <dbReference type="STRING" id="348780.NP_5180A"/>
    <dbReference type="EnsemblBacteria" id="CAI50681">
        <property type="protein sequence ID" value="CAI50681"/>
        <property type="gene ID" value="NP_5180A"/>
    </dbReference>
    <dbReference type="GeneID" id="43500105"/>
    <dbReference type="KEGG" id="nph:NP_5180A"/>
    <dbReference type="HOGENOM" id="CLU_3178677_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="46" mass="5250" checksum="7E19EE3917504ABF" modified="2017-05-10" version="1">MSELLSGPGLFLVFLSLVLLAFPSAFLYMALRERRVFKRLSSRLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZG9</accession>
    <name>A0A1U7EZG9_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Transcription initiation factor IIB</fullName>
            <shortName evidence="10">TFIIB</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">tfbA8</name>
        <name evidence="10" type="synonym">tfb</name>
        <name evidence="14" type="ordered locus">NP_5186A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre-initiation complex (DNA-TBP-TFIIB).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the TFIIB family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324294.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZG9"/>
    <dbReference type="STRING" id="348780.NP_5186A"/>
    <dbReference type="EnsemblBacteria" id="CAI50684">
        <property type="protein sequence ID" value="CAI50684"/>
        <property type="gene ID" value="NP_5186A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703240"/>
    <dbReference type="KEGG" id="nph:NP_5186A"/>
    <dbReference type="eggNOG" id="arCOG01981">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043736_0_1_2"/>
    <dbReference type="OrthoDB" id="7429at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20549">
        <property type="entry name" value="CYCLIN_TFIIB_archaea_like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20550">
        <property type="entry name" value="CYCLIN_TFIIB_archaea_like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000023">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.170:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00383">
        <property type="entry name" value="TF2B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023484">
        <property type="entry name" value="TFIIB_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023486">
        <property type="entry name" value="TFIIB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001658">
        <property type="entry name" value="PRK00423.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF13">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="Zn_Ribbon_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00782">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51134">
        <property type="entry name" value="ZF_TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIB-type" evidence="13">
        <location>
            <begin position="27"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="repeat" description="1" evidence="10">
        <location>
            <begin position="144"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="repeat" description="2" evidence="10">
        <location>
            <begin position="238"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00053882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAI50684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="325" mass="36816" checksum="FD589E3C1C5E7DD5" modified="2017-05-10" version="1">MTRPTRQRERIRETEGETEDEAEDESAETTCPECSSTDIVKDGDRGELVCNDCGLVVEEDHIDPGPEWRAFNHSERQEKSRVGAPTTQTMHDKGLTTQIDWKDQDAYGRSISSEKRSQMHRLRKWQERIRTKDAGERNLQFALSEIDRMSSALGVPRSIREVASVIYRRALDDDLIRGRSIEGVATAALYAACRKEGIPRSLEEISEVSRVERKEIGRTYRYISQELGLEMEPVDPQKYVPRFCSELELSEEVQSKAKDIIETTAEKGLLSGKSPTGYAAAAIYAASLLCNEKKTQREVAEVAQVTEVTIRNRYQEQIEAMGIHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZK3</accession>
    <name>A0A1U7EZK3_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Putative [LysW]-lysine/[LysW]-ornithine hydrolase</fullName>
            <ecNumber evidence="8">3.5.1.130</ecNumber>
            <ecNumber evidence="8">3.5.1.132</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">argE</name>
        <name evidence="8" type="synonym">lysK</name>
        <name evidence="10" type="ordered locus">NP_5266A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the release of L-lysine from [LysW]-gamma-L-lysine and the release of L-ornithine from [LysW]-L-ornithine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>[amino-group carrier protein]-C-terminal-gamma-(L-lysyl)-L-glutamate + H2O = [amino-group carrier protein]-C-terminal-L-glutamate + L-lysine</text>
            <dbReference type="Rhea" id="RHEA:48684"/>
            <dbReference type="Rhea" id="RHEA-COMP:9693"/>
            <dbReference type="Rhea" id="RHEA-COMP:9715"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:78525"/>
            <dbReference type="ChEBI" id="CHEBI:78526"/>
            <dbReference type="EC" id="3.5.1.130"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>[amino-group carrier protein]-C-terminal-gamma-(L-ornithyl)-L-glutamate + H2O = [amino-group carrier protein]-C-terminal-L-glutamate + L-ornithine</text>
            <dbReference type="Rhea" id="RHEA:52676"/>
            <dbReference type="Rhea" id="RHEA-COMP:9693"/>
            <dbReference type="Rhea" id="RHEA-COMP:13328"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:78525"/>
            <dbReference type="ChEBI" id="CHEBI:136763"/>
            <dbReference type="EC" id="3.5.1.132"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <text evidence="8">Binds 2 Zn(2+) or Co(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis; L-arginine biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (Thermus route): step 5/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the peptidase M20A family. LysK subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.130" evidence="8"/>
    <dbReference type="EC" id="3.5.1.132" evidence="8"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324333.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZK3"/>
    <dbReference type="STRING" id="348780.NP_5266A"/>
    <dbReference type="EnsemblBacteria" id="CAI50724">
        <property type="protein sequence ID" value="CAI50724"/>
        <property type="gene ID" value="NP_5266A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702519"/>
    <dbReference type="KEGG" id="nph:NP_5266A"/>
    <dbReference type="eggNOG" id="arCOG01107">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021802_2_0_2"/>
    <dbReference type="OrthoDB" id="156068at2157"/>
    <dbReference type="UniPathway" id="UPA00033">
        <property type="reaction ID" value="UER00039"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00068"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01120">
        <property type="entry name" value="LysK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010175">
        <property type="entry name" value="LysK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050072">
        <property type="entry name" value="Peptidase_M20A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01902">
        <property type="entry name" value="dapE-lys-deAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003367">
        <property type="entry name" value="PRK04443.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808:SF28">
        <property type="entry name" value="[LYSW]-LYSINE_[LYSW]-ORNITHINE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808">
        <property type="entry name" value="ACETYLORNITHINE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0170">Cobalt</keyword>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="9">
        <location>
            <begin position="155"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI50724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="349" mass="37140" checksum="F57C491B47290DE3" modified="2017-05-10" version="1">MSESTPSPDEEARDLLETLVSIPSPTPEETECAEALAGFFESHGRDVFIDEVGNVRAPADDSVLYTSHIDTVPGDIPVRIEDGDGGPALWGRGSVDAKGPLTALAVAAVRTGVSFVGVVGEEVDSRGARHLVADREPPDAVVNGEPSGCDGITLGYRGILQGTYRARSASGHTSRPENNAIQDAVEWWSAVESRFEADDDTPVFERVTPKPVDIDGGVTDDGLAVETTLEAQLRVPPSMSTDAVRAAADEELETGTVTWNDAVEPTMQSPRTPVARAFRAAIRKQGGDPRLLRKTGTADMNIYAGEWDCPMVTYGPGDSDLDHAPDEHLPLAELDRSVAVLETVAEELA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZN6</accession>
    <name>A0A1U7EZN6_NATPD</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">NP_0379C</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CCI69575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
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    <dbReference type="EnsemblBacteria" id="CCI69575">
        <property type="protein sequence ID" value="CCI69575"/>
        <property type="gene ID" value="NP_0379C"/>
    </dbReference>
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    <dbReference type="eggNOG" id="arCOG10972">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_207582_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055947">
        <property type="entry name" value="DUF7525"/>
    </dbReference>
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        <property type="entry name" value="DUF7525"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCI69575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="62" mass="6095" checksum="C7B5349F9E3ED96A" modified="2017-05-10" version="1">METANSTDKGVGFSMLFGVIAVLGAVGLAVFGVTGDQLAAGWSFAVAVAAGGLSVAAYHLYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILV4</accession>
    <name>Q3ILV4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_6272A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007427.1"/>
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    <dbReference type="EnsemblBacteria" id="CAI50916">
        <property type="protein sequence ID" value="CAI50916"/>
        <property type="gene ID" value="NP_6272A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694663"/>
    <dbReference type="KEGG" id="nph:NP_6272A"/>
    <dbReference type="HOGENOM" id="CLU_1763925_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="147" mass="16469" checksum="7576B13BED0B038A" modified="2005-11-08" version="1">MNRRAIIVAGGVFFLSGCGERATDMSNETDHRQTTDEDPNDRDTLAGYGFEDCEYPFNSEKIGPDLESTLRDTDEYDAPIGRGTVLFQYRDGAADAVTTFAQTNGFEIRDRSEHVNYVRFVPDVCTVAEASHKEYITRIEQPISSPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILZ0</accession>
    <name>Q3ILZ0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type transport system ATP-binding protein (Probable substrate branched-chain amino acids)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">livF2</name>
        <name evidence="7" type="synonym">abc06a1</name>
        <name evidence="7" type="ordered locus">NP_6198A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7 8">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324477.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILZ0"/>
    <dbReference type="EnsemblBacteria" id="CAI50879">
        <property type="protein sequence ID" value="CAI50879"/>
        <property type="gene ID" value="NP_6198A"/>
    </dbReference>
    <dbReference type="GeneID" id="69102753"/>
    <dbReference type="KEGG" id="nph:NP_6198A"/>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015807">
        <property type="term" value="P:L-amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03224">
        <property type="entry name" value="ABC_TM1139_LivF_branched"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052156">
        <property type="entry name" value="BCAA_Transport_ATP-bd_LivF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43820:SF7">
        <property type="entry name" value="BRANCHED-CHAIN AMINO ACID TRANSPORT ATP-BINDING PROTEIN LIVF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43820">
        <property type="entry name" value="HIGH-AFFINITY BRANCHED-CHAIN AMINO ACID TRANSPORT ATP-BINDING PROTEIN LIVF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="16"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI50879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="248" mass="26249" checksum="E775AA4D755A9A0C" modified="2005-11-08" version="1">MSSKAELDTGDETATLVAEDIVTGYDGHEVLHGVSIQSHPGVTCIFGPNGSGKSTLLKSLNGVVPLWSGSVQYGDTDLTELGPDKIVGHGVATLPQGGGVFGTMTVEENLKLGAFSVSDKQKTKERKEDVLDAFPALEAKLDTKAKNLSGGQQMMVSLGRAMMTGADTYLMDEPSAGLAPALVDDAFDLIETLVDRGARVILIEQNVVAALRLADYIYILAEGELQFEGTPSELGAEEDLMELYLGIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM19</accession>
    <name>Q3IM19_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_6136A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324448.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM19"/>
    <dbReference type="EnsemblBacteria" id="CAI50848">
        <property type="protein sequence ID" value="CAI50848"/>
        <property type="gene ID" value="NP_6136A"/>
    </dbReference>
    <dbReference type="GeneID" id="43500107"/>
    <dbReference type="KEGG" id="nph:NP_6136A"/>
    <dbReference type="HOGENOM" id="CLU_3003247_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="56" mass="6614" checksum="FEB1265F6FA797E3" modified="2005-11-08" version="1">MTETERNVGELTDEELLAEYKSTVAFRRQEREWGEIAPELDMKIQAIEEEILERMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM20</accession>
    <name>Q3IM20_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">CopG domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_6132A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50846.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939912.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM20"/>
    <dbReference type="EnsemblBacteria" id="CAI50846">
        <property type="protein sequence ID" value="CAI50846"/>
        <property type="gene ID" value="NP_6132A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694618"/>
    <dbReference type="KEGG" id="nph:NP_6132A"/>
    <dbReference type="HOGENOM" id="CLU_161783_0_0_2"/>
    <dbReference type="OrthoDB" id="25654at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22231">
        <property type="entry name" value="RHH_NikR_HicB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="entry name" value="Met repressor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="Ribbon-helix-helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50846.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="98" mass="11030" checksum="647F763ECB1FD9CB" modified="2012-10-03" version="2">MSDADAGANDDPNMTTITLKIPEAFLDDLDATWRAEDFPSRSEFIRWALRDAVKHPTFSRKGWKDIAASEHQLATAEGRTYSSDEIRARLNKDMDAGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-01-23" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM36</accession>
    <name>PSTB3_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphate import ATP-binding protein PstB 3</fullName>
            <ecNumber evidence="1">7.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ABC phosphate transporter 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphate-transporting ATPase 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pstB3</name>
        <name type="synonym">abc15a</name>
        <name type="ordered locus">NP_6098A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>PL131</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the ABC transporter complex PstSACB involved in phosphate import. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>phosphate(out) + ATP + H2O = ADP + 2 phosphate(in) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24440"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.3.2.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The complex is composed of two ATP-binding proteins (PstB), two transmembrane proteins (PstC and PstA) and a solute-binding protein (PstS).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. Phosphate importer (TC 3.A.1.7) family.</text>
    </comment>
    <dbReference type="EC" id="7.3.2.1" evidence="1"/>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324431.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM36"/>
    <dbReference type="SMR" id="Q3IM36"/>
    <dbReference type="EnsemblBacteria" id="CAI50829">
        <property type="protein sequence ID" value="CAI50829"/>
        <property type="gene ID" value="NP_6098A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694669"/>
    <dbReference type="KEGG" id="nph:NP_6098A"/>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="OrthoDB" id="31298at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015415">
        <property type="term" value="F:ATPase-coupled phosphate ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03260">
        <property type="entry name" value="ABC_PstB_phosphate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005670">
        <property type="entry name" value="PstB-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00972">
        <property type="entry name" value="3a0107s01c2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43423">
        <property type="entry name" value="ABC TRANSPORTER I FAMILY MEMBER 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43423:SF1">
        <property type="entry name" value="ABC TRANSPORTER I FAMILY MEMBER 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51238">
        <property type="entry name" value="PSTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0592">Phosphate transport</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000272593" description="Phosphate import ATP-binding protein PstB 3">
        <location>
            <begin position="1"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="1">
        <location>
            <begin position="31"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="63"/>
            <end position="70"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="275" mass="30245" checksum="7D0CA90AE0CBDE02" modified="2005-11-08" version="1">MATQETDDSLISTDVQTDATERGDQPDETVVETKHLDVHYGDEQALDDVSIDIPENKVTALIGPSGCGKSTFLRCINRMNDQIDACRVDGKVVFKGKNVYDDDVDPVALRRKIGQVFQTPNPFPKSIRENVVYGLEVQGEPASDEDVERALRGAALWDEVNNQLDSSGLDLSGGQQQRLCIARAIAPDPEVILMDEPTSALDPVAASKIEDLIDELVEDYTVIIVTHNMQQAARISDKTAVFLTGGNLVEFDDTTNIFENPEDDRVEDYITGKFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM40</accession>
    <name>Q3IM40_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PemK family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_6090A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324427.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM40"/>
    <dbReference type="EnsemblBacteria" id="CAI50825">
        <property type="protein sequence ID" value="CAI50825"/>
        <property type="gene ID" value="NP_6090A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694613"/>
    <dbReference type="KEGG" id="nph:NP_6090A"/>
    <dbReference type="HOGENOM" id="CLU_157708_0_0_2"/>
    <dbReference type="OrthoDB" id="315488at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003477">
        <property type="entry name" value="PemK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011067">
        <property type="entry name" value="Plasmid_toxin/cell-grow_inhib"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02452">
        <property type="entry name" value="PemK_toxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50118">
        <property type="entry name" value="Cell growth inhibitor/plasmid maintenance toxic component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="109" mass="12152" checksum="B763FD5A7EE113A6" modified="2005-11-08" version="1">MAYSQGAIVIADDPFGNNPKRPYLILSNDHVPFHGQEYVAAVITTTARTEAVELTANRLERGQLPRTSYVSPWSVLTLKDWMITKQPAEASDATVDEVRQELDTYLQTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM72</accession>
    <name>Q3IM72_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CopG domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_6014A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM72"/>
    <dbReference type="EnsemblBacteria" id="CAI50787">
        <property type="protein sequence ID" value="CAI50787"/>
        <property type="gene ID" value="NP_6014A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_6014A"/>
    <dbReference type="HOGENOM" id="CLU_150442_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="143" mass="16175" checksum="E33E36F7FDABA992" modified="2005-11-08" version="1">MCKLCAYAETVITLCAEYAHRMSASDDPRRVHFQSPEYLVDRLDAIAELFDKDRTDLLVEAIREYIENTADSETFQELVATKYYDDQLAFETVKQLVGAETAQRLRLLKADLEDEPLDLAAPDDVDVYDGDATAVETTADDEQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMF3</accession>
    <name>TRUD_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable tRNA pseudouridine synthase D</fullName>
            <ecNumber evidence="1">5.4.99.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA pseudouridine(13) synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA pseudouridylate synthase D</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA-uridine isomerase D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">truD</name>
        <name type="ordered locus">NP_5228A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Could be responsible for synthesis of pseudouridine from uracil-13 in transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>uridine(13) in tRNA = pseudouridine(13) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42540"/>
            <dbReference type="Rhea" id="RHEA-COMP:10105"/>
            <dbReference type="Rhea" id="RHEA-COMP:10106"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase TruD family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.27" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324315.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMF3"/>
    <dbReference type="SMR" id="Q3IMF3"/>
    <dbReference type="STRING" id="348780.NP_5228A"/>
    <dbReference type="EnsemblBacteria" id="CAI50705">
        <property type="protein sequence ID" value="CAI50705"/>
        <property type="gene ID" value="NP_5228A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702304"/>
    <dbReference type="KEGG" id="nph:NP_5228A"/>
    <dbReference type="eggNOG" id="arCOG04252">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005281_4_1_2"/>
    <dbReference type="OrthoDB" id="1798at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160150">
        <property type="term" value="F:tRNA pseudouridine(13) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1510.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.20">
        <property type="entry name" value="TruD, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01082">
        <property type="entry name" value="TruD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001656">
        <property type="entry name" value="PsdUridine_synth_TruD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020119">
        <property type="entry name" value="PsdUridine_synth_TruD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011760">
        <property type="entry name" value="PsdUridine_synth_TruD_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042214">
        <property type="entry name" value="TruD_catalytic"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002158">
        <property type="entry name" value="PRK00984.2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00094">
        <property type="entry name" value="tRNA_TruD_broad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326:SF21">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE PUS7L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01142">
        <property type="entry name" value="TruD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037016">
        <property type="entry name" value="Pseudouridin_synth_euk_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50984">
        <property type="entry name" value="TRUD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01268">
        <property type="entry name" value="UPF0024"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000230160" description="Probable tRNA pseudouridine synthase D">
        <location>
            <begin position="1"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="TRUD" evidence="1">
        <location>
            <begin position="170"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01082"/>
        </source>
    </evidence>
    <sequence length="435" mass="47903" checksum="3A90970EE4F57952" modified="2005-11-08" version="1">MRDSHPVEQTVGIEYYVTETDGTGGRLRDRPADFRVREREAFGADCRPLDADPGSYPHLVFRATLREWDTNDFASAVSNALGVSRERVSWAGTKDKHAVTTQLFSVRHDDAALPDLDGADIEPIGRAGRPVLFGDLAGNEFELVIRDPDRPEHAEATAAELCDFGGGEAGVPNYFGTQRFGSRRPITHRVGLDVLDGDWEAAAVRYVCESSEREPERTQEVREGIDADRDWAAAGERLPGSLRFERAIANRLAEGAESPDDYRAALEELPSNLQRMFVNAAQSYVFNRILSERLRRGLPFDEPVVGDVVCFSDSDGNPDPDRTQTVTESRLETVRRHCERGRAFVTAPLVGTETVFGDGEPAEITREVLADVDVSPTDFELPGEFGSSGTRRAVLVTTDLTVEQEPLTLSFSLPKGSYATVVAREFLKADPEALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IML2</accession>
    <name>Q3IML2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KaiC domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_5110A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324256.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IML2"/>
    <dbReference type="STRING" id="348780.NP_5110A"/>
    <dbReference type="EnsemblBacteria" id="CAI50646">
        <property type="protein sequence ID" value="CAI50646"/>
        <property type="gene ID" value="NP_5110A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702259"/>
    <dbReference type="KEGG" id="nph:NP_5110A"/>
    <dbReference type="eggNOG" id="arCOG01171">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023669_2_2_2"/>
    <dbReference type="OrthoDB" id="49590at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014774">
        <property type="entry name" value="KaiC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010624">
        <property type="entry name" value="KaiC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637">
        <property type="entry name" value="UPF0273 PROTEIN TM_0370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637:SF1">
        <property type="entry name" value="UPF0273 PROTEIN TM_0370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06745">
        <property type="entry name" value="ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51146">
        <property type="entry name" value="KAIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KaiC" evidence="3">
        <location>
            <begin position="1"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="231" mass="25163" checksum="063DEEC14DA0EF37" modified="2005-11-08" version="1">MRVSSGVAGFDDLVAGGFPVGRLYVLSGPPGSGKTTFSAQFLVDGAKNGESCLFISMHESRADIAHDMSGYDIGFEQVLESDRLTFVDVFSSEGKRLFQSPGKHRDASGLINRLTGFIDSNDIDRVVVDSTMLLDHFLNDAESNIIQFLTSLKRTDATVLLISEMTDPSAYADEHYLAHGVVFLHNFLEDGGMQRGIQVLKMRGTAIDTDIHEVSFGDDGLSVGGRRLQTH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMY7</accession>
    <name>RL4_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">50S ribosomal protein L4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl4</name>
        <name type="ordered locus">NP_4856A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Forms part of the polypeptide exit tunnel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324131.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMY7"/>
    <dbReference type="SMR" id="Q3IMY7"/>
    <dbReference type="STRING" id="348780.NP_4856A"/>
    <dbReference type="EnsemblBacteria" id="CAI50519">
        <property type="protein sequence ID" value="CAI50519"/>
        <property type="gene ID" value="NP_4856A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703145"/>
    <dbReference type="KEGG" id="nph:NP_4856A"/>
    <dbReference type="eggNOG" id="arCOG04071">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026535_0_0_2"/>
    <dbReference type="OrthoDB" id="10737at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01328_A">
        <property type="entry name" value="Ribosomal_uL4_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002136">
        <property type="entry name" value="Ribosomal_uL4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023574">
        <property type="entry name" value="Ribosomal_uL4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013000">
        <property type="entry name" value="Ribosomal_uL4_euk/arc_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045240">
        <property type="entry name" value="Ribosomal_uL4_euk/arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019970">
        <property type="entry name" value="Ribosomall_uL4-arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03672">
        <property type="entry name" value="rpl4p_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19431">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00573">
        <property type="entry name" value="Ribosomal_L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52166">
        <property type="entry name" value="Ribosomal protein L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00939">
        <property type="entry name" value="RIBOSOMAL_L1E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000242470" description="Large ribosomal subunit protein uL4">
        <location>
            <begin position="1"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="44"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="92"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="248" mass="26772" checksum="0472CB1FC5D245B3" modified="2005-11-08" version="1">MKATVRDLDGDDAGEVELPAVFETDYRPDLIKRAVLAAQANRIQDTGTDEYAGLRTPAESPGSGRGMAHVPRQNGRAREVPQAVSGRPAHPPKAEKDRGLDINTKERKLATRSAIAATANADRVEERGHEFDEETDLPLVVSDEFEDLVKTQEAVDVLEALGVYDDIERAEDGKTVRAGRGTTRGRKYTEPKSVLVVTSDDQSLAARNLAGSDVATADEVNVEDLAPGTQAGRLTLWTESALAEVAER</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-09-11" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IN29</accession>
    <name>GCSH_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable glycine cleavage system H protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gcvH</name>
        <name type="ordered locus">NP_4772A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
        <text evidence="1">Binds 1 lipoyl cofactor covalently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GcvH family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324089.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IN29"/>
    <dbReference type="SMR" id="Q3IN29"/>
    <dbReference type="STRING" id="348780.NP_4772A"/>
    <dbReference type="EnsemblBacteria" id="CAI50477">
        <property type="protein sequence ID" value="CAI50477"/>
        <property type="gene ID" value="NP_4772A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702743"/>
    <dbReference type="KEGG" id="nph:NP_4772A"/>
    <dbReference type="eggNOG" id="arCOG01303">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097408_2_0_2"/>
    <dbReference type="OrthoDB" id="9810at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06848">
        <property type="entry name" value="GCS_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00272">
        <property type="entry name" value="GcvH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002930">
        <property type="entry name" value="GCV_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033753">
        <property type="entry name" value="GCV_H/Fam206"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017453">
        <property type="entry name" value="GCV_H_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00527">
        <property type="entry name" value="gcvH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002270">
        <property type="entry name" value="PRK01202.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11715">
        <property type="entry name" value="GLYCINE CLEAVAGE SYSTEM H PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11715:SF3">
        <property type="entry name" value="GLYCINE CLEAVAGE SYSTEM H PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01597">
        <property type="entry name" value="GCV_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0450">Lipoyl</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000302472" description="Probable glycine cleavage system H protein">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="2">
        <location>
            <begin position="24"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-lipoyllysine" evidence="1">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01066"/>
        </source>
    </evidence>
    <sequence length="126" mass="13919" checksum="2DF9211335269246" modified="2005-11-08" version="1">MSFDVPADRRYLESHEWAQPDDDVVRVGITDFAQDELGDIVFVELPSVGDRIEHEAEFGVIESIKAVSDLYAPVSGEVVAVNDDLEDAPELVNDDPFGDGWLLEVEADGDDYESLLTADEYEAQIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IN66</accession>
    <name>Q3IN66_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">NP_4694A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IN66"/>
    <dbReference type="EnsemblBacteria" id="CAI50438">
        <property type="protein sequence ID" value="CAI50438"/>
        <property type="gene ID" value="NP_4694A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_4694A"/>
    <dbReference type="eggNOG" id="arCOG01174">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023669_4_2_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051347">
        <property type="entry name" value="Circadian_clock_KaiC-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030665">
        <property type="entry name" value="KaiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014774">
        <property type="entry name" value="KaiC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010624">
        <property type="entry name" value="KaiC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42926">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42926:SF1">
        <property type="entry name" value="CIRCADIAN CLOCK OSCILLATOR PROTEIN KAIC 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06745">
        <property type="entry name" value="ATPase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039117">
        <property type="entry name" value="KaiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01874">
        <property type="entry name" value="DNAREPAIRADA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51146">
        <property type="entry name" value="KAIC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="KaiC" evidence="8">
        <location>
            <begin position="9"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="KaiC" evidence="8">
        <location>
            <begin position="247"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="477"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="487"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI50438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="496" mass="54046" checksum="80AC71F4824F267B" modified="2005-11-08" version="1">MSNPSPTMMQGDSGVVGLNQLLRGGYLRNRMYLIVGTHGTGKTTLCSHFVEAGLDAGETALFVHGEESASELRENAAQFGIDIAGAEFLDLGPDSAFFEKDETYDLVDASEVEGKRYTEQIREAITEIDPDRLVIDPITQLRYIEASDYHYRKRVLSLIRFLKEHEITVLGTTSVSDTHSTASEILSISDGVVRLSRSETGRRIEVEKNRGRGQQSGTHGVEIRAHGVEVFPKLTPPESPAERRGLDPVATGVGELDSLTGGGFEHGTTTFISGPPGVGKTTVGAYFLFWAAQQGETAAVYLFEERIETFEKRCRAIGLPIDALRDDGRLKLTRIEPNAMSAEEFANIVRTDVVDGGVTTAMIDGFSGYTTSIQGEQDTLKRDLHALLRHLNELDVAVFMTEAVHQITGLTSATSRNISPLADNLLFLTYVELGGSLGRVIGMLKKRTGSFESGIREFEITDEGVQLGAPLDHLQGLLEGTPSTRGHRLESSDTEP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-05-20" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q3INI7</accession>
    <name>RL10_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">50S ribosomal protein L10</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Acidic ribosomal protein P0 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl10</name>
        <name evidence="1" type="synonym">rplP0</name>
        <name type="ordered locus">NP_4450A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Forms a heptameric L10(L12)2(L12)2(L12)2 complex, where L10 forms an elongated spine to which the L12 dimers bind in a sequential fashion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323931.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3INI7"/>
    <dbReference type="SMR" id="Q3INI7"/>
    <dbReference type="STRING" id="348780.NP_4450A"/>
    <dbReference type="EnsemblBacteria" id="CAI50316">
        <property type="protein sequence ID" value="CAI50316"/>
        <property type="gene ID" value="NP_4450A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703119"/>
    <dbReference type="KEGG" id="nph:NP_4450A"/>
    <dbReference type="eggNOG" id="arCOG04288">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053173_0_0_2"/>
    <dbReference type="OrthoDB" id="30930at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05795">
        <property type="entry name" value="Ribosomal_P0_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00280">
        <property type="entry name" value="Ribosomal_uL10_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050323">
        <property type="entry name" value="Ribosomal_protein_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040637">
        <property type="entry name" value="Ribosomal_uL10-like_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043164">
        <property type="entry name" value="Ribosomal_uL10-like_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_uL10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022909">
        <property type="entry name" value="Ribosomal_uL10_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003097">
        <property type="entry name" value="PRK04019.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003098">
        <property type="entry name" value="PRK04019.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17777">
        <property type="entry name" value="RL10P_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="Ribosomal protein L10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000078823" description="Large ribosomal subunit protein uL10">
        <location>
            <begin position="1"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="286"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="286"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="307"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="354" mass="37197" checksum="2A6D9F47D9A22727" modified="2005-11-08" version="1">MSAEARKTETIPEWKQEEIDELVAFLERYESVGVVDITGIPSRQLQDMRRDLHGTAALRVSRNTLMERALNEGGDGLGELVEHVEGQVGLIGTNDNPFGLYQQLEESKTPAPINAGEVAPNDIVIPEGDTGVDPGPFVGDLQQVGANARIEGGSIKVVEDSTVLSAGEEVSSDLSNVLSELGIEPKEVGLDLRGVSSEGVLFSPEELDIDVESYRTDIESAASAARNLSVNAEYPTARTAPSMLAKAAGEAKSVGLSAAVESPDLADDLVSKADAQVRALAAQIDDEEALPEELQDVEQPAAADSAAEADDEDDTGNVEQTDESDADDADDADDADDADEEDGDGGDALGDMFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IP22</accession>
    <name>Q3IP22_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">M20 family amidohydrolase (Homolog to indole-3-acetyl-aspartate hydrolase)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_4078A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">The Mn(2+) ion enhances activity.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323746.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IP22"/>
    <dbReference type="STRING" id="348780.NP_4078A"/>
    <dbReference type="EnsemblBacteria" id="CAI50130">
        <property type="protein sequence ID" value="CAI50130"/>
        <property type="gene ID" value="NP_4078A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701962"/>
    <dbReference type="KEGG" id="nph:NP_4078A"/>
    <dbReference type="eggNOG" id="arCOG01108">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023257_2_1_2"/>
    <dbReference type="OrthoDB" id="247417at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016805">
        <property type="term" value="F:dipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071713">
        <property type="term" value="F:para-aminobenzoyl-glutamate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046657">
        <property type="term" value="P:folic acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017439">
        <property type="entry name" value="Amidohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052030">
        <property type="entry name" value="Peptidase_M20/M20A_hydrolases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01891">
        <property type="entry name" value="amidohydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30575">
        <property type="entry name" value="PEPTIDASE M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30575:SF3">
        <property type="entry name" value="PEPTIDASE M20 DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005962">
        <property type="entry name" value="Pept_M20D_amidohydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0464">Manganese</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="2">
        <location>
            <begin position="220"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="393"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR005962-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="423" mass="45525" checksum="F794FB9151500F18" modified="2005-11-08" version="1">MYVPRERLIELRRAFHQHPEPAWREFWTTAKVVSELRDIGVDELHIGREALSDERLAVPDEEELASWRERAREAGADPDLLEELADGWTGCVAAIDCGEGPTVGLRVDIDGLPREESTADDHIPAREGFRSETGAMHACGHDAHMTFGLGVAEAITETDFSGTLKLFFQPAEEVIGGGKPMAESGHLDDVDYLFAVHVGLDYPTGEVVAGIDDFLAITQFYTTFEGHPGHAGARPEEGRNAVQAMATAVQNLYGIERHSEGESRVNAGHVGGGTATNIIPEEAFIHGEVRGETTDVRDYTFERAEQVFEGAAVSHDCTVDIEIEGDAPSAESDAELVELVTDAARGHPDVERATRRGEIGGSEDATFLMQRVQDTGGLATYACIGTDHPGGHHTATFDVDEASLSLGVDVLADAIAATAETMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IPK0</accession>
    <name>Q3IPK0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">dolichyl-phosphooligosaccharide-protein glycotransferase</fullName>
            <ecNumber evidence="6">2.4.99.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Oligosaccharyl transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">aglB</name>
        <name evidence="22" type="ordered locus">NP_3720A</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>an archaeal dolichyl phosphooligosaccharide + [protein]-L-asparagine = an archaeal dolichyl phosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.</text>
            <dbReference type="EC" id="2.4.99.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the STT3 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.99.21" evidence="6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323568.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IPK0"/>
    <dbReference type="STRING" id="348780.NP_3720A"/>
    <dbReference type="CAZy" id="GT66">
        <property type="family name" value="Glycosyltransferase Family 66"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI49951">
        <property type="protein sequence ID" value="CAI49951"/>
        <property type="gene ID" value="NP_3720A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703218"/>
    <dbReference type="KEGG" id="nph:NP_3720A"/>
    <dbReference type="eggNOG" id="arCOG02043">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008803_0_0_2"/>
    <dbReference type="OrthoDB" id="82393at2157"/>
    <dbReference type="BRENDA" id="2.4.99.18">
        <property type="organism ID" value="3575"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004576">
        <property type="term" value="F:oligosaccharyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054479">
        <property type="entry name" value="AglB-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041154">
        <property type="entry name" value="AglB_P1"/>
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    <dbReference type="InterPro" id="IPR003674">
        <property type="entry name" value="Oligo_trans_STT3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026410">
        <property type="entry name" value="OlisacTrfase_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048307">
        <property type="entry name" value="STT3_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04154">
        <property type="entry name" value="archaeo_STT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872:SF1">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT STT3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22627">
        <property type="entry name" value="AglB_core-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18079">
        <property type="entry name" value="AglB_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02516">
        <property type="entry name" value="STT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 22" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 22" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="23"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="123"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="153"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="223"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="303"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="338"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="395"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="426"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="448"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="486"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="Oligosaccharyl transferase STT3 N-terminal" evidence="19">
        <location>
            <begin position="53"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="AglB-like core" evidence="21">
        <location>
            <begin position="521"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="Archaeal glycosylation protein B peripheral" evidence="20">
        <location>
            <begin position="800"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="901"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="925"/>
            <end position="957"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF18079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF22627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="CAI49951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="957" mass="104568" checksum="C82FA4DBDD8B4EE7" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IPM6</accession>
    <name>NDK_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleoside diphosphate kinase</fullName>
            <shortName evidence="1">NDK</shortName>
            <shortName evidence="1">NDP kinase</shortName>
            <ecNumber evidence="1">2.7.4.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Nucleoside-2-P kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ndk</name>
        <name type="ordered locus">NP_3666A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'-deoxyribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:44640"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-diphosphate + ATP = a ribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:18113"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NDK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.6" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323542.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IPM6"/>
    <dbReference type="SMR" id="Q3IPM6"/>
    <dbReference type="STRING" id="348780.NP_3666A"/>
    <dbReference type="EnsemblBacteria" id="CAI49924">
        <property type="protein sequence ID" value="CAI49924"/>
        <property type="gene ID" value="NP_3666A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702971"/>
    <dbReference type="KEGG" id="nph:NP_3666A"/>
    <dbReference type="eggNOG" id="arCOG04313">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060216_6_3_2"/>
    <dbReference type="OrthoDB" id="6874at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006228">
        <property type="term" value="P:UTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04413">
        <property type="entry name" value="NDPk_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.141:FF:000002">
        <property type="entry name" value="Nucleoside diphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="entry name" value="Nucleoside diphosphate kinase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00451">
        <property type="entry name" value="NDP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001564">
        <property type="entry name" value="Nucleoside_diP_kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001908">
        <property type="entry name" value="PRK00668.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11349">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01243">
        <property type="entry name" value="NUCDPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00562">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="Nucleoside diphosphate kinase, NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51374">
        <property type="entry name" value="NDPK_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0546">Nucleotide metabolism</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000226589" description="Nucleoside diphosphate kinase">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="active site" description="Pros-phosphohistidine intermediate" evidence="1">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00451"/>
        </source>
    </evidence>
    <sequence length="153" mass="17291" checksum="21C17C069CA9A013" modified="2005-11-08" version="1">MADTERTFVMVKPDGVQRGLIGDIVSRFEDRGLKLVAGKFMQIDDELAREHYAEHVDKPFFDELKEFITSGPVFAMVWEGQDAVAQVRTMMGETDPAESAPGTIRGDFGLDLGRNVIHGSDTEPGSAEREIGLFFDDDELQDYERIDEPWLYE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IPX0</accession>
    <name>DNLI_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA ligase</fullName>
            <ecNumber evidence="1">6.5.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Polydeoxyribonucleotide synthase [ATP]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lig</name>
        <name type="ordered locus">NP_3474A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.1" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323448.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IPX0"/>
    <dbReference type="SMR" id="Q3IPX0"/>
    <dbReference type="STRING" id="348780.NP_3474A"/>
    <dbReference type="EnsemblBacteria" id="CAI49828">
        <property type="protein sequence ID" value="CAI49828"/>
        <property type="gene ID" value="NP_3474A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702886"/>
    <dbReference type="KEGG" id="nph:NP_3474A"/>
    <dbReference type="eggNOG" id="arCOG01347">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005138_6_0_2"/>
    <dbReference type="OrthoDB" id="31274at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006273">
        <property type="term" value="P:lagging strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07901">
        <property type="entry name" value="Adenylation_DNA_ligase_Arch_LigB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07972">
        <property type="entry name" value="OBF_DNA_ligase_Arch_LigB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3260.10:FF:000007">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3260.10">
        <property type="entry name" value="DNA ligase, ATP-dependent, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00407">
        <property type="entry name" value="DNA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050191">
        <property type="entry name" value="ATP-dep_DNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022865">
        <property type="entry name" value="DNA_ligae_ATP-dep_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000977">
        <property type="entry name" value="DNA_ligase_ATP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012309">
        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012308">
        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036599">
        <property type="entry name" value="DNA_ligase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054890">
        <property type="entry name" value="LigA_Halo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00574">
        <property type="entry name" value="dnl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041331">
        <property type="entry name" value="LigA_Halo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674:SF7">
        <property type="entry name" value="DNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674">
        <property type="entry name" value="DNA LIGASE 1/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04675">
        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117018">
        <property type="entry name" value="ATP-dependent DNA ligase DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00697">
        <property type="entry name" value="DNA_LIGASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000049874" description="DNA ligase">
        <location>
            <begin position="1"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="active site" description="N6-AMP-lysine intermediate" evidence="1">
        <location>
            <position position="254"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="343"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="414"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="420"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00407"/>
        </source>
    </evidence>
    <sequence length="548" mass="58865" checksum="621F22FEEA73D814" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-26" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IQG6</accession>
    <name>AROA_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-phosphoshikimate 1-carboxyvinyltransferase</fullName>
            <ecNumber evidence="1">2.5.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">5-enolpyruvylshikimate-3-phosphate synthase</fullName>
            <shortName evidence="1">EPSP synthase</shortName>
            <shortName evidence="1">EPSPS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aroA</name>
        <name type="ordered locus">NP_3078A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1-carboxyvinyl)-3-phosphoshikimate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21256"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="EC" id="2.5.1.19"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:21257"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; chorismate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the EPSP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.19" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323252.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IQG6"/>
    <dbReference type="SMR" id="Q3IQG6"/>
    <dbReference type="STRING" id="348780.NP_3078A"/>
    <dbReference type="EnsemblBacteria" id="CAI49630">
        <property type="protein sequence ID" value="CAI49630"/>
        <property type="gene ID" value="NP_3078A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702526"/>
    <dbReference type="KEGG" id="nph:NP_3078A"/>
    <dbReference type="eggNOG" id="arCOG04134">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024321_0_0_2"/>
    <dbReference type="OrthoDB" id="43788at2157"/>
    <dbReference type="UniPathway" id="UPA00053"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003866">
        <property type="term" value="F:3-phosphoshikimate 1-carboxyvinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01556">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.10">
        <property type="entry name" value="Enolpyruvate transferase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00210">
        <property type="entry name" value="EPSP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001986">
        <property type="entry name" value="Enolpyruvate_Tfrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036968">
        <property type="entry name" value="Enolpyruvate_Tfrase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006264">
        <property type="entry name" value="EPSP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023193">
        <property type="entry name" value="EPSP_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
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        <property type="entry name" value="aroA"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="AROM/DEHYDROQUINATE SYNTHASE"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR21090:SF5">
        <property type="entry name" value="PENTAFUNCTIONAL AROM POLYPEPTIDE"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00275">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000505">
        <property type="entry name" value="EPSPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="EPT/RTPC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00104">
        <property type="entry name" value="EPSP_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00885">
        <property type="entry name" value="EPSP_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000071738" description="3-phosphoshikimate 1-carboxyvinyltransferase">
        <location>
            <begin position="1"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="320"/>
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        <location>
            <position position="20"/>
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        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
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        <location>
            <position position="20"/>
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        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
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            <position position="21"/>
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        <ligand>
            <name>3-phosphoshikimate</name>
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        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
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        <location>
            <position position="90"/>
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        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
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    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="118"/>
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        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
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        <ligand>
            <name>3-phosphoshikimate</name>
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        <ligand>
            <name>phosphoenolpyruvate</name>
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            <name>3-phosphoshikimate</name>
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        <location>
            <position position="351"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="392"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00210"/>
        </source>
    </evidence>
    <sequence length="438" mass="44955" checksum="21E5FC32FF60BEE8" modified="2005-11-08" version="1">MDAHITPSTVNGTVRAPPSKSYTHRAILAAGYGDGATVKNPLFSADTRATARAVEAYGGTTEQADDDLEVTGFDGTPGTPKDVINCANSGTTMRLTTATGALVDGLAVLTGDESLRSRPQGPLLDALDQLGARAESTRENGQAPLVVGGDIDGGELAIRGDVSSQYITGLLMAGAVTESGIDIELTTALKSAPYVDITLEVLADFGVDAEVIGGDDSEVRSAGADGFRVDGGQSYAPTDGTYHVPGDFSSMSYLLAAGALAADEEVVVAGAHPSAQGDAAIVSILESMGADIEWNRDDGRITVRQSSLSGVEVGVADTPDLLPTIAVLGAAADGETRITDCEHVRLKETDRVAAMATELERLGVETDEYEDELVVYGGGIDGGTVAGHDDHRIVMSLAVAGLVADGEVTIKNADHVDVSFPSFFETLADLGVGVETGA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-30" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IQL3</accession>
    <name>PURL_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribosylformylglycinamidine synthase subunit PurL</fullName>
            <shortName evidence="1">FGAM synthase</shortName>
            <ecNumber evidence="1">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Formylglycinamide ribonucleotide amidotransferase subunit II</fullName>
            <shortName evidence="1">FGAR amidotransferase II</shortName>
            <shortName evidence="1">FGAR-AT II</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glutamine amidotransferase PurL</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphoribosylformylglycinamidine synthase subunit II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purL</name>
        <name type="ordered locus">NP_2982A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + L-glutamine + ATP + H2O = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FGAMS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323206.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IQL3"/>
    <dbReference type="SMR" id="Q3IQL3"/>
    <dbReference type="STRING" id="348780.NP_2982A"/>
    <dbReference type="EnsemblBacteria" id="CAI49582">
        <property type="protein sequence ID" value="CAI49582"/>
        <property type="gene ID" value="NP_2982A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703073"/>
    <dbReference type="KEGG" id="nph:NP_2982A"/>
    <dbReference type="eggNOG" id="arCOG00641">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003100_0_1_2"/>
    <dbReference type="OrthoDB" id="8251at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02203">
        <property type="entry name" value="PurL_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02204">
        <property type="entry name" value="PurL_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00420">
        <property type="entry name" value="PurL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010074">
        <property type="entry name" value="PRibForGlyAmidine_synth_PurL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041609">
        <property type="entry name" value="PurL_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01736">
        <property type="entry name" value="FGAM_synth_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002290">
        <property type="entry name" value="PRK01213.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43555">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43555:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18072">
        <property type="entry name" value="FGAR-AT_linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001587">
        <property type="entry name" value="FGAM_synthase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000236677" description="Phosphoribosylformylglycinamidine synthase subunit PurL">
        <location>
            <begin position="1"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="34"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="86"/>
            <end position="89"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="305"/>
            <end position="307"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="480"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="517"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="518"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="520"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="713" mass="74250" checksum="BFF44EB3212E842A" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IRT7</accession>
    <name>Q3IRT7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tubulin-like protein CetZ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cetZ2</name>
        <name evidence="6" type="synonym">cetZ</name>
        <name evidence="9" type="synonym">ftsZ3</name>
        <name evidence="9" type="ordered locus">NP_2128A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in cell shape control.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the CetZ family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322783.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IRT7"/>
    <dbReference type="STRING" id="348780.NP_2128A"/>
    <dbReference type="EnsemblBacteria" id="CAI49155">
        <property type="protein sequence ID" value="CAI49155"/>
        <property type="gene ID" value="NP_2128A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702729"/>
    <dbReference type="KEGG" id="nph:NP_2128A"/>
    <dbReference type="eggNOG" id="arCOG02202">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058152_0_0_2"/>
    <dbReference type="OrthoDB" id="329751at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02202">
        <property type="entry name" value="CetZ_tubulin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01946">
        <property type="entry name" value="CetZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032907">
        <property type="entry name" value="CetZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048737">
        <property type="entry name" value="CetZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045061">
        <property type="entry name" value="FtsZ/CetZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314">
        <property type="entry name" value="CELL DIVISION PROTEIN FTSZ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314:SF10">
        <property type="entry name" value="TUBULIN-LIKE PROTEIN CETZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21011">
        <property type="entry name" value="CetZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00423">
        <property type="entry name" value="CELLDVISFTSZ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0133">Cell shape</keyword>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="8">
        <location>
            <begin position="2"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="357"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="363"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="10"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="110"/>
            <end position="112"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI49155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="396" mass="40751" checksum="B2B9D9D9D36D2009" modified="2005-11-08" version="1">MKLVAIGAGQAGGKVLDRLLEHDARHPGTFLAHAVAVNTAEPDLQALDAVPEESRLLIGQSTVGGHGTGTDPETGRECAREAESEIQSAIDHAPTSSIDGFVVIAGLGGGTGSGAAPVIAERLRAVYAEPVYGLGILPTADEGGLYSRNAADSLQAFVGATDGLLVFDNDAFKSSGETLSEGYGAINEEIATRFGTLFSAGELEALGDDIAESVVDASEIINTLEGLVSVGYASEELPDDAGDDGGLLGRVLGGSDDDSIGSDAVVQIPALVRRAALGKLTLPCNLASTSKALLIVAGPPEHLSRKGIDNARSWLESEIDCREVRAGDYPLPDADRVAALVVLSGVTDVPRIDRMQSQAVDAETAETSEAESEDAMQDLIEYGDDSDGDGATDGHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ISJ8</accession>
    <name>Q3ISJ8_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">fructose-bisphosphate aldolase</fullName>
            <ecNumber evidence="2">4.1.2.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">fba1</name>
        <name evidence="4" type="ordered locus">NP_1594A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DeoC/FbaB aldolase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.13" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322522.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ISJ8"/>
    <dbReference type="STRING" id="348780.NP_1594A"/>
    <dbReference type="EnsemblBacteria" id="CAI48888">
        <property type="protein sequence ID" value="CAI48888"/>
        <property type="gene ID" value="NP_1594A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702691"/>
    <dbReference type="KEGG" id="nph:NP_1594A"/>
    <dbReference type="eggNOG" id="arCOG04044">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057069_2_2_2"/>
    <dbReference type="OrthoDB" id="6329at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00958">
        <property type="entry name" value="DhnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002915">
        <property type="entry name" value="DeoC/FbaB/LacD_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050456">
        <property type="entry name" value="DeoC/FbaB_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041720">
        <property type="entry name" value="FbaB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47916:SF1">
        <property type="entry name" value="3-HYDROXY-5-PHOSPHONOOXYPENTANE-2,4-DIONE THIOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47916">
        <property type="entry name" value="FRUCTOSE-BISPHOSPHATE ALDOLASE CLASS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01791">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038992">
        <property type="entry name" value="Aldolase_Ia"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01133">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with dihydroxyacetone-P" evidence="3">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR038992-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="264" mass="28392" checksum="7B156FE73AC51594" modified="2005-11-08" version="1">MIPIDDTPLCRDGKALILAYDHGLEHGPVDFKDVPESADPERTFEAATHPAVTSVAVQKGVAEAYYPSYEDDVDLMVKLNGTSNLWMGEPDSAVNCSVDYAAELGASSVGFTLYGGSNKEVEMAEEFRDVHEAARDHGLPTVMWSYPRGQGLKNDTKPSTIAYAARLALELGADIAKVKHPGSKSAMEDAVRMAGKTKVVMSGGSKTTDREFLESVKQTIDAGGKGLAVGRNVWQRENPTRILDALEAVIFEEASVDEALDAAE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ISQ0</accession>
    <name>DAPA_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4-hydroxy-tetrahydrodipicolinate synthase</fullName>
            <shortName evidence="1">HTPA synthase</shortName>
            <ecNumber evidence="1">4.3.3.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dapA</name>
        <name type="ordered locus">NP_1490A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-aspartate 4-semialdehyde + pyruvate = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:34171"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="4.3.3.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DapA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Was originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.7" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322470.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ISQ0"/>
    <dbReference type="SMR" id="Q3ISQ0"/>
    <dbReference type="STRING" id="348780.NP_1490A"/>
    <dbReference type="EnsemblBacteria" id="CAI48836">
        <property type="protein sequence ID" value="CAI48836"/>
        <property type="gene ID" value="NP_1490A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702656"/>
    <dbReference type="KEGG" id="nph:NP_1490A"/>
    <dbReference type="eggNOG" id="arCOG04172">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049343_7_1_2"/>
    <dbReference type="OrthoDB" id="33636at2157"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00017"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008675">
        <property type="term" value="F:2-dehydro-3-deoxy-phosphogluconate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008840">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00950">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00418">
        <property type="entry name" value="DapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005263">
        <property type="entry name" value="DapA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002220">
        <property type="entry name" value="DapA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020624">
        <property type="entry name" value="Schiff_base-form_aldolases_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00674">
        <property type="entry name" value="dapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128:SF66">
        <property type="entry name" value="4-HYDROXY-2-OXOGLUTARATE ALDOLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128">
        <property type="entry name" value="DIHYDRODIPICOLINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00701">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001365">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00146">
        <property type="entry name" value="DHPICSNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01130">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00665">
        <property type="entry name" value="DHDPS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0457">Lysine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0704">Schiff base</keyword>
    <feature type="chain" id="PRO_1000050228" description="4-hydroxy-tetrahydrodipicolinate synthase">
        <location>
            <begin position="1"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="1">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <feature type="site" description="Part of a proton relay during catalysis" evidence="1">
        <location>
            <position position="48"/>
        </location>
    </feature>
    <feature type="site" description="Part of a proton relay during catalysis" evidence="1">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="294" mass="32041" checksum="EE661ADF6B230734" modified="2005-11-08" version="1">MTHDTFTGVFPAMTTPFDDEERIDHETLRDHAQRLEAAGVDGLVPVGTTGESATMSHDEHIEVVETVVDAVDDVPVIAGSGSNNTREALELSERAADAGADGLLLISPYYNIPEPEGMETHYRTVADAVDLPQIIYNVPGRTGRNIAVETAVSLAEHENIVGYKAASGDLNRVGEVIERTREENFEVLSGDDALTLPIIAQGGTGCISVSANVEPERTVALVGAALEGDFTRARELQYELGDLFRTLFIETNPIPINEAMDMRDIHSSTMRSPLNPLQPEHREQLREVLADLER</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-07-22" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ITN8</accession>
    <name>DTDA_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">D-aminoacyl-tRNA deacylase</fullName>
            <ecNumber evidence="1">3.1.1.96</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>D-tyrosyl-tRNA(Tyr) deacylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dtdA</name>
        <name type="ordered locus">NP_0808A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">D-aminoacyl-tRNA deacylase with broad substrate specificity. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a D-aminoacyl-tRNA + H2O = a tRNA + a D-alpha-amino acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13953"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:10124"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59871"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:79333"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>glycyl-tRNA(Ala) + H2O = tRNA(Ala) + glycine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53744"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:13640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DtdA deacylase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.96" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322131.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ITN8"/>
    <dbReference type="SMR" id="Q3ITN8"/>
    <dbReference type="STRING" id="348780.NP_0808A"/>
    <dbReference type="EnsemblBacteria" id="CAI48495">
        <property type="protein sequence ID" value="CAI48495"/>
        <property type="gene ID" value="NP_0808A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700968"/>
    <dbReference type="KEGG" id="nph:NP_0808A"/>
    <dbReference type="eggNOG" id="arCOG01616">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_610619_0_0_2"/>
    <dbReference type="OrthoDB" id="9863at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051499">
        <property type="term" value="F:D-aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106026">
        <property type="term" value="F:Gly-tRNA(Ala) deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019478">
        <property type="term" value="P:D-amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10700">
        <property type="entry name" value="AF0625-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.50">
        <property type="entry name" value="AF0625-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00562">
        <property type="entry name" value="Deacylase_DtdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018033">
        <property type="entry name" value="Deacylase_DtdA_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007508">
        <property type="entry name" value="DtdA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011435">
        <property type="entry name" value="PRK14866.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34667">
        <property type="entry name" value="D-AMINOACYL-TRNA DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34667:SF1">
        <property type="entry name" value="D-AMINOACYL-TRNA DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04414">
        <property type="entry name" value="tRNA_deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142535">
        <property type="entry name" value="AF0625-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000345225" description="D-aminoacyl-tRNA deacylase">
        <location>
            <begin position="1"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00562"/>
        </source>
    </evidence>
    <sequence length="441" mass="46763" checksum="9DF179D302829D83" modified="2005-11-08" version="1">MLAIVVSRADEASEHIGRKLRSLADWTEHEDERRADGAGGGTYYRTDRAELRIFDDLHIHLDGAASAFEEPDLLVFASRHSGDTGPLLTAHATGNFGPAEYGGGDGSLARAAPNALSAVRDAFETHAPDDYDVGIECTHHGPSTVGCSSLFVELGSGPDQWQDSEGAAAVARSILSLRGVEPHTERTVVGFGGGHYAPRFDRVLTDTDWGVGHVAADWSLTELGDPRDSRAVIAKAFQASGTEFALVDGDRPELEAVIEDLGFEVLSETFLQETTGVPLSLVGRLEDDCGPIDDGLRLGEPATGYDGEFVTEELPDELLDEANGVDRTAVREAADAVALAYGTADGGSLAVGPVALAAADDYRSLVESLAAVLDTKYDSVEIEASTVIAHREAFDPELARDAGVDEGPAFGKLSSGASVDVDGETVTPDDVHRVRERRFEF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IU15</accession>
    <name>PCNA_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA polymerase sliding clamp</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Proliferating cell nuclear antigen homolog</fullName>
            <shortName evidence="1">PCNA</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pcn</name>
        <name type="ordered locus">NP_0554A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Sliding clamp subunit that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase and other proteins to DNA during high-speed replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotrimer. The subunits circularize to form a toroid; DNA passes through its center. Replication factor C (RFC) is required to load the toroid on the DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PCNA family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322004.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IU15"/>
    <dbReference type="SMR" id="Q3IU15"/>
    <dbReference type="STRING" id="348780.NP_0554A"/>
    <dbReference type="EnsemblBacteria" id="CAI48368">
        <property type="protein sequence ID" value="CAI48368"/>
        <property type="gene ID" value="NP_0554A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703009"/>
    <dbReference type="KEGG" id="nph:NP_0554A"/>
    <dbReference type="eggNOG" id="arCOG00488">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043978_1_1_2"/>
    <dbReference type="OrthoDB" id="14749at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030337">
        <property type="term" value="F:DNA polymerase processivity factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006275">
        <property type="term" value="P:regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00577">
        <property type="entry name" value="PCNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00317">
        <property type="entry name" value="DNApol_clamp_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046938">
        <property type="entry name" value="DNA_clamp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000730">
        <property type="entry name" value="Pr_cel_nuc_antig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022649">
        <property type="entry name" value="Pr_cel_nuc_antig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022659">
        <property type="entry name" value="Pr_cel_nuc_antig_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022648">
        <property type="entry name" value="Pr_cel_nuc_antig_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002222">
        <property type="entry name" value="PRK01115.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352:SF0">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02747">
        <property type="entry name" value="PCNA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00705">
        <property type="entry name" value="PCNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00339">
        <property type="entry name" value="PCNACYCLIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55979">
        <property type="entry name" value="DNA clamp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01251">
        <property type="entry name" value="PCNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000019178" description="DNA polymerase sliding clamp">
        <location>
            <begin position="1"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00317"/>
        </source>
    </evidence>
    <sequence length="247" mass="26955" checksum="B627ED1BD3685C11" modified="2005-11-08" version="1">MFKAIVSADTLGAALDSVSVLVDECKVRLDEEGLTIRAVDPANVGMVDLELSASAFESYETDGGVIGVNLDRLEDIVGMADSGQLVHLDLDEETRKLHISLDGLEYTLALIDPDSIRQEPDLPDLDLSSEIVIEGADIDRAVTAADMVSDHIALGVDPDAEEFYVDAEGDTDDVHLELDREDLIDLTPGEARSLFSLDYLKDMNKAIPKDAEVTMELGEEFPVKMHFDFAEGDGHVTYMLAPRIQSD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IUM7</accession>
    <name>RS7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein uS7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">30S ribosomal protein S7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps7</name>
        <name type="ordered locus">NP_0124A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321792.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IUM7"/>
    <dbReference type="SMR" id="Q3IUM7"/>
    <dbReference type="STRING" id="348780.NP_0124A"/>
    <dbReference type="EnsemblBacteria" id="CAI48153">
        <property type="protein sequence ID" value="CAI48153"/>
        <property type="gene ID" value="NP_0124A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703186"/>
    <dbReference type="KEGG" id="nph:NP_0124A"/>
    <dbReference type="eggNOG" id="arCOG04254">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063975_0_0_2"/>
    <dbReference type="OrthoDB" id="45346at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14867">
        <property type="entry name" value="uS7_Eukaryote"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.455.10">
        <property type="entry name" value="Ribosomal protein S7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00480_A">
        <property type="entry name" value="Ribosomal_uS7_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000235">
        <property type="entry name" value="Ribosomal_uS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026018">
        <property type="entry name" value="Ribosomal_uS7_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020606">
        <property type="entry name" value="Ribosomal_uS7_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023798">
        <property type="entry name" value="Ribosomal_uS7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036823">
        <property type="entry name" value="Ribosomal_uS7_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005716">
        <property type="entry name" value="Ribosomal_uS7_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003106">
        <property type="entry name" value="PRK04027.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01028">
        <property type="entry name" value="uS7_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11205">
        <property type="entry name" value="RIBOSOMAL PROTEIN S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00177">
        <property type="entry name" value="Ribosomal_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002122">
        <property type="entry name" value="RPS7p_RPS7a_RPS5e_RPS7o"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47973">
        <property type="entry name" value="Ribosomal protein S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00052">
        <property type="entry name" value="RIBOSOMAL_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000226543" description="Small ribosomal subunit protein uS7">
        <location>
            <begin position="1"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="203" mass="22631" checksum="017E46233E1A2A64" modified="2005-11-08" version="1">MSSEAPEPDAPASTDDERVSAQLFGEWEIGEIEYDDPSTRRYITVTPIAHTMGRHSEKQFKKSEISIVERLINRLMQTDENTGKKQQATKIVREAFDIVAERTDENPVQVLVTAVENAGPREETVRLKYGGISVPKAVDVAPQRRVDLALKFIAEGTYNDSFKTTTPVEEALAHQLIGAANYDLQAYPVSQKEEQERVAAAAR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-12-06" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IUT9</accession>
    <name>YA57_NATPD</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein NP_1057A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">NP_1057A</name>
        <name type="ORF">NP1057E</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAJ32463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322255.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IUT9"/>
    <dbReference type="SMR" id="Q3IUT9"/>
    <dbReference type="EnsemblBacteria" id="CAJ32463">
        <property type="protein sequence ID" value="CAJ32463"/>
        <property type="gene ID" value="NP_1057E"/>
    </dbReference>
    <dbReference type="GeneID" id="3701042"/>
    <dbReference type="KEGG" id="nph:NP_1057E"/>
    <dbReference type="eggNOG" id="arCOG09322">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2695878_0_0_2"/>
    <dbReference type="OrthoDB" id="211838at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058337">
        <property type="entry name" value="DUF8024"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26067">
        <property type="entry name" value="DUF8024"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000076365" description="Uncharacterized protein NP_1057A">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="73" mass="7366" checksum="7C0F868BC7DE107E" modified="2005-11-08" version="1">MVEIQTGLRENTVGAVEQFAEIASIDPLVASMILSGAIIITVAVVAFGALTLGAIGASIKRGLSGSEEPNQPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETA7</accession>
    <name>A0A1U7ETA7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">3,4-dihydroxy-2-butanone 4-phosphate synthase</fullName>
            <shortName evidence="6">DHBP synthase</shortName>
            <ecNumber evidence="6">4.1.99.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ribB</name>
        <name evidence="7" type="ordered locus">NP_0074A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>D-ribulose 5-phosphate = (2S)-2-hydroxy-3-oxobutyl phosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18457"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="4.1.99.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="6">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="6">Binds 2 divalent metal cations per subunit. Magnesium or manganese.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DHBP synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.12" evidence="6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321767.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETA7"/>
    <dbReference type="STRING" id="348780.NP_0074A"/>
    <dbReference type="EnsemblBacteria" id="CAI48128">
        <property type="protein sequence ID" value="CAI48128"/>
        <property type="gene ID" value="NP_0074A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703104"/>
    <dbReference type="KEGG" id="nph:NP_0074A"/>
    <dbReference type="eggNOG" id="arCOG01320">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020273_3_2_2"/>
    <dbReference type="OrthoDB" id="25735at2157"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00399"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008686">
        <property type="term" value="F:3,4-dihydroxy-2-butanone-4-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.870.10">
        <property type="entry name" value="DHBP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000422">
        <property type="entry name" value="DHBP_synthase_RibB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00506">
        <property type="entry name" value="ribB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21327:SF46">
        <property type="entry name" value="3,4-DIHYDROXY-2-BUTANONE 4-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21327">
        <property type="entry name" value="GTP CYCLOHYDROLASE II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00926">
        <property type="entry name" value="DHBP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="YrdC/RibB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 6" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0686">Riboflavin biosynthesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="241" mass="25126" checksum="E47DA9EEC0BB2B67" modified="2017-05-10" version="1">MSRSSDTATADRGVDAAVSAFAAGQPVLVHDAADREGEVDLVYPAGAVAPADVARMRNDAGGLICVALSDAVCTAWELPFRQTLVDHPAGASHELDYDERSSFSLTVNHRDTFTGITDSDRALTIAELGAAADDIRRARTVAGEDATGAIAPDAEAFAERFRAPGHVHLLRGAPDLLDDREGHTELGLALAEAAGVAPAVVVCEMLDDETGEALSPTAARAYADRNGFPYVEGSDIVSRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETB7</accession>
    <name>A0A1U7ETB7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Dihydrolipoyl dehydrogenase</fullName>
            <ecNumber evidence="2 10">1.8.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">lpdA</name>
        <name evidence="13" type="ordered locus">NP_0104A</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + NAD(+) = N(6)-[(R)-lipoyl]-L-lysyl-[protein] + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15045"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="EC" id="1.8.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="10">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="10">The active site is a redox-active disulfide bond.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.4" evidence="2 10"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321782.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETB7"/>
    <dbReference type="STRING" id="348780.NP_0104A"/>
    <dbReference type="EnsemblBacteria" id="CAI48143">
        <property type="protein sequence ID" value="CAI48143"/>
        <property type="gene ID" value="NP_0104A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702889"/>
    <dbReference type="KEGG" id="nph:NP_0104A"/>
    <dbReference type="eggNOG" id="arCOG01068">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016755_0_1_2"/>
    <dbReference type="OrthoDB" id="27922at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004148">
        <property type="term" value="F:dihydrolipoyl dehydrogenase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006103">
        <property type="term" value="P:2-oxoglutarate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.30:FF:000001">
        <property type="entry name" value="Dihydrolipoyl dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050151">
        <property type="entry name" value="Class-I_Pyr_Nuc-Dis_Oxidored"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006258">
        <property type="entry name" value="Lipoamide_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01350">
        <property type="entry name" value="lipoamide_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22912:SF160">
        <property type="entry name" value="DIHYDROLIPOYL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22912">
        <property type="entry name" value="DISULFIDE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 10" id="KW-0274">FAD</keyword>
    <keyword evidence="3 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6 10" id="KW-0520">NAD</keyword>
    <keyword evidence="5 10 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 10" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="12">
        <location>
            <begin position="11"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="11">
        <location>
            <begin position="352"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAI48143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="474" mass="48932" checksum="2872BF9F6900EB69" modified="2017-05-10" version="1">MVVGDISTGTELLVVGGGPGGYVAAIRAGQLDVDVTLVEADAYGGTCLNEGCIPSKALIHVATLADEVESAEAMGLHARADVDMQGLVDWKDGVVDQLTSGVEKLCKANGVSLVEGRAEFVDGETARIAHGGEGQGSETVTFDSAVIATGSRPIELPGVEFDGEHILDSSDVLDLRSVPESMTVVGGGYIGMELSTMLAKLGCDVTIVEMLDDILPGYTEDITRLVRNRAESLGVAFSFGERATGWEPAGDGVRLDTETEDGETASYTAENVLVAVGREGAADTCNVEALGLEPRNDGTLETDATTQTSVDGIYAVGDVAGEPMLAHKAYREGHVAAEVVAGEPSRLDYQAIPSAVFTDPEIGTVGLTEAEAEAEGFDPVVGEMPLRASGRALTLGADEGFVRIVADAETGFVLGGQIVGPEAAELVAEVGLAIELGARLEDIAATVHTHPTLSEAVAEAAANARDSAIHTLNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETC3</accession>
    <name>A0A1U7ETC3_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Type-4 uracil-DNA glycosylase</fullName>
            <ecNumber evidence="3">3.2.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">udg1</name>
        <name evidence="13" type="ordered locus">NP_0084A</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil.</text>
            <dbReference type="EC" id="3.2.2.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the uracil-DNA glycosylase (UDG) superfamily. Type 4 (UDGa) family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.27" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321772.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETC3"/>
    <dbReference type="STRING" id="348780.NP_0084A"/>
    <dbReference type="EnsemblBacteria" id="CAI48133">
        <property type="protein sequence ID" value="CAI48133"/>
        <property type="gene ID" value="NP_0084A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700756"/>
    <dbReference type="KEGG" id="nph:NP_0084A"/>
    <dbReference type="eggNOG" id="arCOG00905">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044815_1_3_2"/>
    <dbReference type="OrthoDB" id="8612at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10030">
        <property type="entry name" value="UDG-F4_TTUDGA_SPO1dp_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="entry name" value="Uracil-DNA glycosylase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051536">
        <property type="entry name" value="UDG_Type-4/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005273">
        <property type="entry name" value="Ura-DNA_glyco_family4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00758">
        <property type="entry name" value="UDG_fam4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33693:SF1">
        <property type="entry name" value="TYPE-4 URACIL-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33693">
        <property type="entry name" value="TYPE-5 URACIL-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00986">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00987">
        <property type="entry name" value="UreE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="8 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uracil-DNA glycosylase-like" evidence="12">
        <location>
            <begin position="25"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAI48133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="194" mass="20874" checksum="26E6C9AC0E9D9182" modified="2017-05-10" version="1">MTDDVDVTDCRRCSELCASRSRIVNGVGPTDADLLFVGEAPGETEDEVGEPFVGRSGDVLDEGLRDRGLSRRDIRITNCVRCRPPDNRDPTAEERSNCRPYLDAEIAAVDPDVVVALGKVPAEHLLDRSVAVTEAAGAIYDARIDGTARRVLVCVHPAATLYDPSQREQFDAALDAAVGFLDDSAGGQSRLGEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETM2</accession>
    <name>A0A1U7ETM2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PUA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0340A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321899.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETM2"/>
    <dbReference type="STRING" id="348780.NP_0340A"/>
    <dbReference type="EnsemblBacteria" id="CAI48261">
        <property type="protein sequence ID" value="CAI48261"/>
        <property type="gene ID" value="NP_0340A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700823"/>
    <dbReference type="KEGG" id="nph:NP_0340A"/>
    <dbReference type="eggNOG" id="arCOG00985">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090468_1_1_2"/>
    <dbReference type="OrthoDB" id="27972at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21154">
        <property type="entry name" value="PUA_MJ1432-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.400.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022430">
        <property type="entry name" value="CHP03684"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016437">
        <property type="entry name" value="MCT-1/Tma20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03684">
        <property type="entry name" value="arCOG00985"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011154">
        <property type="entry name" value="PRK14560.1-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22798:SF0">
        <property type="entry name" value="MALIGNANT T-CELL-AMPLIFIED SEQUENCE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22798">
        <property type="entry name" value="MCT-1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005067">
        <property type="entry name" value="Tma_RNA-bind_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PUA" evidence="1">
        <location>
            <begin position="77"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="159" mass="17173" checksum="7F33514F07607A51" modified="2017-05-10" version="1">MQVKSRHHLRSDEIDALEERLADRLGVTPDGDSYERVEFDDVDREVVLVDGEPLVASFDDDLFLTVRGANEYAPDSHVVTVDSGAVSFVSDGANVMRPGITEATADIEPGDLVVIVEEAHGKALGVGRAEADGDDMVGDSGKVVETLHHVGDDLYEFHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETV7</accession>
    <name>A0A1U7ETV7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">TetR family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">NP_0534A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321994.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETV7"/>
    <dbReference type="STRING" id="348780.NP_0534A"/>
    <dbReference type="EnsemblBacteria" id="CAI48358">
        <property type="protein sequence ID" value="CAI48358"/>
        <property type="gene ID" value="NP_0534A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700888"/>
    <dbReference type="KEGG" id="nph:NP_0534A"/>
    <dbReference type="eggNOG" id="arCOG02643">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_15_3_2"/>
    <dbReference type="OrthoDB" id="135877at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.10">
        <property type="entry name" value="Tetracycline Repressor, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039538">
        <property type="entry name" value="BetI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050109">
        <property type="entry name" value="HTH-type_TetR-like_transc_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30055:SF234">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR BETI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30055">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR RUTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13977">
        <property type="entry name" value="TetR_C_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="Tetracyclin repressor-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="6">
        <location>
            <begin position="3"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="5">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="196" mass="22078" checksum="707CDC5BBEF01A2D" modified="2017-05-10" version="1">MVEETRERIMEATYRALCDNGYAALTMQDIADEGDCSKSLLHYHFDTKEELLASLLAYLLERFEERVSADPETPRDQLADLVGLFLFGDGERSREEHRAFHTALLELRAQAIHNETFREQLAENDERIHATVAAVVERGVEEGQFRPVDAERLAAHVLAAIQGARIRWVTLGNEAGLELVYGALVEGLIDGWLVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETW2</accession>
    <name>A0A1U7ETW2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7981 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0568A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322011.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETW2"/>
    <dbReference type="STRING" id="348780.NP_0568A"/>
    <dbReference type="EnsemblBacteria" id="CAI48375">
        <property type="protein sequence ID" value="CAI48375"/>
        <property type="gene ID" value="NP_0568A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700891"/>
    <dbReference type="KEGG" id="nph:NP_0568A"/>
    <dbReference type="eggNOG" id="arCOG06419">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_184943_2_1_2"/>
    <dbReference type="OrthoDB" id="307419at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058287">
        <property type="entry name" value="DUF7981"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25938">
        <property type="entry name" value="DUF7981"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7981" evidence="2">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="68" mass="7126" checksum="E06C80AA861625B9" modified="2017-05-10" version="1">MKPRRKSALLWGAVAALSFLVLHQGYLLAGGAFLGVGPVAAVTVAVFVGGAAVSYYAEGRFGLFGWRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETW4</accession>
    <name>A0A1U7ETW4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TRAP-type transport system periplasmic substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tp53a</name>
        <name evidence="1" type="ordered locus">NP_0544A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321999.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETW4"/>
    <dbReference type="STRING" id="348780.NP_0544A"/>
    <dbReference type="EnsemblBacteria" id="CAI48363">
        <property type="protein sequence ID" value="CAI48363"/>
        <property type="gene ID" value="NP_0544A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703339"/>
    <dbReference type="KEGG" id="nph:NP_0544A"/>
    <dbReference type="eggNOG" id="arCOG01801">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033215_0_0_2"/>
    <dbReference type="OrthoDB" id="27995at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd13567">
        <property type="entry name" value="PBP2_TtGluBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011852">
        <property type="entry name" value="TRAP_TAXI"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02122">
        <property type="entry name" value="TRAP_TAXI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42941">
        <property type="entry name" value="SLL1037 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42941:SF1">
        <property type="entry name" value="SLL1037 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16868">
        <property type="entry name" value="NMT1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="344" mass="35772" checksum="A4447BB2C1975E8F" modified="2017-05-10" version="1">MVDTSNLDRRSLLKGVGVAGIAGLAGCTGNGDGNGNGNGNGNGNGNGNGNGNGDDGAAGEAIGWHAGGTGGTYYPFSQELKDIVETETNHTLQVQSTGASVENVGSLASGDADFALIQNDIATFATEGRGIAAFEGNPIENLRGVATLYPETIHILTRPAADIETVSDLEGMRVNTGDSGSGTQVNALQILESAAGLTPDDFDEQNSEFAQAADQIRDGDVDAAFIVGGWPVGAIEELATTADVDLLELSDDERATLQEDAQWFTDDTVPGGTYPGIDEDTDTVSVQAMIATREEYDEDIVEEVTAAIFDNEDQITIQSEFVSRDTAQDGMPIELHPGAAAYFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETY9</accession>
    <name>A0A1U7ETY9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">2-isopropylmalate synthase / (R)-citramalate synthase</fullName>
            <ecNumber evidence="7">2.3.1.182</ecNumber>
            <ecNumber evidence="7">2.3.3.13</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">leuA2</name>
        <name evidence="7" type="synonym">cimA</name>
        <name evidence="7" type="ordered locus">NP_0624A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the alpha-IPM synthase/homocitrate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.182" evidence="7"/>
    <dbReference type="EC" id="2.3.3.13" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322039.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETY9"/>
    <dbReference type="STRING" id="348780.NP_0624A"/>
    <dbReference type="EnsemblBacteria" id="CAI48403">
        <property type="protein sequence ID" value="CAI48403"/>
        <property type="gene ID" value="NP_0624A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702877"/>
    <dbReference type="KEGG" id="nph:NP_0624A"/>
    <dbReference type="eggNOG" id="arCOG02092">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022158_0_1_2"/>
    <dbReference type="OrthoDB" id="6555at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003852">
        <property type="term" value="F:2-isopropylmalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.260:FF:000001">
        <property type="entry name" value="2-isopropylmalate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013709">
        <property type="entry name" value="2-isopropylmalate_synth_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054691">
        <property type="entry name" value="LeuA/HCS_post-cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036230">
        <property type="entry name" value="LeuA_allosteric_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002085">
        <property type="entry name" value="PRK00915.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42880:SF2">
        <property type="entry name" value="(R)-CITRAMALATE SYNTHASE CIMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42880">
        <property type="entry name" value="HOMOCITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22617">
        <property type="entry name" value="HCS_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08502">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00917">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110921">
        <property type="entry name" value="2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="6">
        <location>
            <begin position="19"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="511" mass="53675" checksum="2F95759C47596B52" modified="2017-05-10" version="1">MTTLFGDHPATAPLEDQDVQFLDTTLRDGEQAPGISLTPEEKADIARELDAANVSVIEAGSACTGPGERETIARVADLGLDARVTSFARGVKNDIDLAVDCGVDGVNLVVPASDRHVEQKVDTTHDAVVEKTVELVEYATDHGLWVEVLGEDGSRADLDFLEELMGAAIDAGADRICAPDTVGHATPDRMLDIYARLSELGPTSTHTHDDLGLAVTNALVGVAAGADLVHGTINGIGERAGNVAIEEVAIALDHGYGVETLETERLYDLGQLVSTKTGVPLAPNKAVVGENAFTHESGIHTDGTLKDDAMYEPYPPEKVGRERRLVLGKHAGRAGVEAALAEHDIEVTDDELSEVVDRVKRLGDRGKRVTDADLLTIAEEVQGREHDRRVELVDLTAASGSGTPTASVRLEVDGTERVASGTGSGPVDAAIAAVRSALGSAADAQLESYHVDAITGGTDAVVTVEVEMSRNDRTVTVAASDSDITTASVRAMVDALDRLLADESEPVLADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETZ0</accession>
    <name>A0A1U7ETZ0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DUF192 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0628A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48405.3"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939430.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETZ0"/>
    <dbReference type="STRING" id="348780.NP_0628A"/>
    <dbReference type="EnsemblBacteria" id="CAI48405">
        <property type="protein sequence ID" value="CAI48405"/>
        <property type="gene ID" value="NP_0628A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700910"/>
    <dbReference type="KEGG" id="nph:NP_0628A"/>
    <dbReference type="eggNOG" id="arCOG03113">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097039_4_2_2"/>
    <dbReference type="OrthoDB" id="64208at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1140">
        <property type="entry name" value="Protein of unknown function DUF192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003795">
        <property type="entry name" value="DUF192"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038695">
        <property type="entry name" value="Saro_0823-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37953">
        <property type="entry name" value="UPF0127 PROTEIN MJ1496"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37953:SF1">
        <property type="entry name" value="UPF0127 PROTEIN MJ1496"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02643">
        <property type="entry name" value="DUF192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48405.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="118" mass="12596" checksum="87C27355E08D9FBD" modified="2017-05-10" version="1">MRVVHETDGGGRPLATKVDVADSLVSQGLGLMFRRSIPDDYALVFPFDEAAPRTLHMVFVPFDIDAIWLEAGDVRAVERLGGWTGYGRAVADTVIELPAGAADAVSVGDRVRIDGLTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETZ5</accession>
    <name>A0A1U7ETZ5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Receiver/sensor/bat box HTH-10 family transcription regulator Bat (Homolog to bacterioopsin activator)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">bat</name>
        <name evidence="12" type="ordered locus">NP_0654A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322054.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETZ5"/>
    <dbReference type="STRING" id="348780.NP_0654A"/>
    <dbReference type="EnsemblBacteria" id="CAI48418">
        <property type="protein sequence ID" value="CAI48418"/>
        <property type="gene ID" value="NP_0654A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702548"/>
    <dbReference type="KEGG" id="nph:NP_0654A"/>
    <dbReference type="eggNOG" id="arCOG02276">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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        <location>
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            <position position="60"/>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI48418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="681" mass="73156" checksum="1A1667F14302959D" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU07</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">NP_0676A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
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            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
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    <dbReference type="EnsemblBacteria" id="CAI48429">
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        <property type="gene ID" value="NP_0676A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700926"/>
    <dbReference type="KEGG" id="nph:NP_0676A"/>
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="entry name" value="EMC6-arch"/>
    </dbReference>
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        <property type="entry name" value="EMC6_arch"/>
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    </dbReference>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="105" mass="10999" checksum="24606E3CE455088C" modified="2017-05-10" version="1">MATDTAERRIAHVRGVTVTAIACLAGVAAAVVSAAVADGAGDTLGLYVLVGGIIVQFPLLRVLEMAGLQLDVDDFGAKDYLYIAFMTFAMWFVCWTVLLTTGASV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU95</accession>
    <name>A0A1U7EU95_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nucleoside recognition protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0860A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
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            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322157.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU95"/>
    <dbReference type="STRING" id="348780.NP_0860A"/>
    <dbReference type="EnsemblBacteria" id="CAI48521">
        <property type="protein sequence ID" value="CAI48521"/>
        <property type="gene ID" value="NP_0860A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700980"/>
    <dbReference type="KEGG" id="nph:NP_0860A"/>
    <dbReference type="eggNOG" id="arCOG00360">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048086_0_0_2"/>
    <dbReference type="OrthoDB" id="51620at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038880">
        <property type="entry name" value="MJ0871-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38139">
        <property type="entry name" value="GATE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38139:SF1">
        <property type="entry name" value="NUCLEOSIDE TRANSPORTER_FEOB GTPASE GATE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="17"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="133"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="226"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="306"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="327" mass="33847" checksum="BE186826752A29DC" modified="2017-05-10" version="1">MEPSVGALLLGEVLPRILTIAAAIAIGVAFANLLVAFGAIQYIAALSKPLTGPANLPDEVGSAILTTAASTTAGYGMLAEYRDSGLLDDRATLVAVTINTFFGFVQHIFTFYAPVLIPILGLKVGVLYVSMRAGISLAITATGVLAGALLLSSRNVTPGAMSDGDSDTETPGGDSSPTREKLLDAGRSGLEKTWEILPRLAIVYTLVVVGTTYYDLESLTGGAADPLAALTGLPSAAVPVIVVYAFDTTSGALTIAPLIEDGVFTARTAVATMLIGGIVSFTVSTFKRSIPFQYGIWGAEFGTKVIIVNTSLKVLWIAIAVTLLLAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUA9</accession>
    <name>A0A1U7EUA9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Transport protein (Probable substrate ammonium)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">amtB</name>
        <name evidence="14" type="synonym">tp39</name>
        <name evidence="14" type="ordered locus">NP_0922A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in the uptake of ammonium/ammonia (NH(4)(+)/NH(3)). Transport is electrogenic.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the P(II) protein family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322188.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUA9"/>
    <dbReference type="STRING" id="348780.NP_0922A"/>
    <dbReference type="EnsemblBacteria" id="CAI48552">
        <property type="protein sequence ID" value="CAI48552"/>
        <property type="gene ID" value="NP_0922A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703315"/>
    <dbReference type="KEGG" id="nph:NP_0922A"/>
    <dbReference type="eggNOG" id="arCOG02305">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG04397">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_33_1_2"/>
    <dbReference type="OrthoDB" id="10960at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097272">
        <property type="term" value="P:ammonium homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018047">
        <property type="entry name" value="Ammonium_transpt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002187">
        <property type="entry name" value="N-reg_PII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017918">
        <property type="entry name" value="N-reg_PII_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730">
        <property type="entry name" value="AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730:SF6">
        <property type="entry name" value="AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00543">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00340">
        <property type="entry name" value="PIIGLNB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00938">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="GlnB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01219">
        <property type="entry name" value="AMMONIUM_TRANSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00638">
        <property type="entry name" value="PII_GLNB_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51343">
        <property type="entry name" value="PII_GLNB_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="211"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="258"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="286"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="310"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="388"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="13">
        <location>
            <begin position="23"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-UMP-tyrosine" evidence="10">
        <location>
            <position position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR602187-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAI48552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="586" mass="60614" checksum="7407A1215D4A1396" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUG8</accession>
    <name>A0A1U7EUG8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NUDIX family hydrolase / eIF-2B domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_1050A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2B alpha/beta/delta subunits family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322251.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUG8"/>
    <dbReference type="STRING" id="348780.NP_1050A"/>
    <dbReference type="EnsemblBacteria" id="CAI48616">
        <property type="protein sequence ID" value="CAI48616"/>
        <property type="gene ID" value="NP_1050A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703268"/>
    <dbReference type="KEGG" id="nph:NP_1050A"/>
    <dbReference type="eggNOG" id="arCOG01127">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_568173_0_0_2"/>
    <dbReference type="OrthoDB" id="27639at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046523">
        <property type="term" value="F:S-methyl-5-thioribose-1-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43475">
        <property type="entry name" value="METHYLTHIORIBOSE-1-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43475:SF3">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_2G14290)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="2">
        <location>
            <begin position="2"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="409" mass="43237" checksum="43B749B9C981DC55" modified="2017-05-10" version="1">METEHVVTCFLRHGTEVLFVERSDAVGTYAGRWGGVSGYTEGNPDEAARWEIDEEVGLLSAATLVRRGEPVTVDDDECGRRWMVHPYLFAVEHRDVTPNEEVAAVEWLQPPVIRDRETVPKLWAAYRAVGPAVETVRMDTDRGAAALSLRALEALRDRAAEVDDYAAVAETGRALLAARPSMAVVRNRISRVLSAAGRTPMAVCQAAMDACEAALEAGAEAASRAADSLGERVLTLSRSGTVEAAILEAEPSRVVVAESRPGREGVGTAERLAEAGIDVTVCADAAVTAALSTADIETVVFGADTVLADGSVINKVGSHPAALAAADTDIECLAVCSRDKMSPTTAADIETGPPEAVYDGDADVSVYNPAFERVPASLVDGIVTEEGRLPTAAAAAIAERHADHRDWRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUJ1</accession>
    <name>A0A1U7EUJ1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CbtB family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1096A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322275.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUJ1"/>
    <dbReference type="STRING" id="348780.NP_1096A"/>
    <dbReference type="EnsemblBacteria" id="CAI48639">
        <property type="protein sequence ID" value="CAI48639"/>
        <property type="gene ID" value="NP_1096A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701050"/>
    <dbReference type="KEGG" id="nph:NP_1096A"/>
    <dbReference type="eggNOG" id="arCOG06354">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2911619_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012667">
        <property type="entry name" value="CbtB_put"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09489">
        <property type="entry name" value="CbtB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="61" mass="6512" checksum="2B3CAEF57D06B0D8" modified="2017-05-10" version="1">MSSHETVIDRLELARSDLSLAQVAAALGFGAAIAFTLVFLQEPLVHDATHNFRHAAGIACH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUK7</accession>
    <name>A0A1U7EUK7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cobalt-precorrin-4 C11-methyltransferase</fullName>
            <ecNumber evidence="8">2.1.1.271</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cbiF</name>
        <name evidence="8" type="synonym">cobM</name>
        <name evidence="8" type="ordered locus">NP_1120A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.271" evidence="8"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322287.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUK7"/>
    <dbReference type="STRING" id="348780.NP_1120A"/>
    <dbReference type="EnsemblBacteria" id="CAI48651">
        <property type="protein sequence ID" value="CAI48651"/>
        <property type="gene ID" value="NP_1120A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702570"/>
    <dbReference type="KEGG" id="nph:NP_1120A"/>
    <dbReference type="eggNOG" id="arCOG00645">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011276_7_1_2"/>
    <dbReference type="OrthoDB" id="6633at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046026">
        <property type="term" value="F:precorrin-4 C11-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11641">
        <property type="entry name" value="Precorrin-4_C11-MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006362">
        <property type="entry name" value="Cbl_synth_CobM/CibF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050161">
        <property type="entry name" value="Siro_Cobalamin_biosynth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45790:SF4">
        <property type="entry name" value="COBALT-PRECORRIN-4 C(11)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45790">
        <property type="entry name" value="SIROHEME SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="3 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="7">
        <location>
            <begin position="37"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI48651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="293" mass="31380" checksum="7C2BBAB067177D0B" modified="2017-05-10" version="1">MTETDPQAAIDAERKKHAHERDERVYEHVAGDSQEGIPFIGAGPGNPRLLTVAGKEAVESADLVVHAGSLVNSELLEAYCDDAELVNSVGKDLEELVPLMRDAYEDGRSVVRLHSGDPAIYGAALEQMDALEHEGVPTYIVPGVTAAFAASATLRTQLTLNETANHVVFTRPQGKTLDPEDDHLAEFVEMGDVTACVYLGTHAVAETMERLVEEGADPETPVSVVYHASWPDEDIIEGTIGDIGEKVEAAGYRASALVLIGDAAAGAGYERSYLYGGWAKRGETESDTDDTNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUR8</accession>
    <name>A0A1U7EUR8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1288A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322370.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUR8"/>
    <dbReference type="STRING" id="348780.NP_1288A"/>
    <dbReference type="EnsemblBacteria" id="CAI48735">
        <property type="protein sequence ID" value="CAI48735"/>
        <property type="gene ID" value="NP_1288A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701102"/>
    <dbReference type="KEGG" id="nph:NP_1288A"/>
    <dbReference type="HOGENOM" id="CLU_2597842_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="79" mass="8541" checksum="0A371D20A122CD79" modified="2017-05-10" version="1">MSLRRRSAGLIDKAPWTVRVLGFTLAVSVAFLLCIGVLFAAFSLAESLFPSERLRSLFALGVFVVVVLGVTQALVVMSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUS1</accession>
    <name>A0A1U7EUS1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">rio1</name>
        <name evidence="14" type="synonym">pkn2</name>
        <name evidence="14" type="ordered locus">NP_1312A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322382.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUS1"/>
    <dbReference type="STRING" id="348780.NP_1312A"/>
    <dbReference type="EnsemblBacteria" id="CAI48747">
        <property type="protein sequence ID" value="CAI48747"/>
        <property type="gene ID" value="NP_1312A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701111"/>
    <dbReference type="KEGG" id="nph:NP_1312A"/>
    <dbReference type="eggNOG" id="arCOG01180">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018693_3_3_2"/>
    <dbReference type="OrthoDB" id="31344at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05145">
        <property type="entry name" value="RIO1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051272">
        <property type="entry name" value="RIO-type_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054876">
        <property type="entry name" value="RIO1_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018934">
        <property type="entry name" value="RIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000687">
        <property type="entry name" value="RIO_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018935">
        <property type="entry name" value="RIO_kinase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041310">
        <property type="entry name" value="Prot_Kin_Rio1_Halo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45723">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01163">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00090">
        <property type="entry name" value="RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01245">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RIO kinase" evidence="13">
        <location>
            <begin position="50"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAI48747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="289" mass="32528" checksum="49569D829C3D20B5" modified="2017-05-10" version="1">MTEDDGLLSADDGEQPGDEWEELDVSDTEADRIARAQDREFLEFRKRIKNTEQFKVEASVFDDATLGALYKLVQDSYVDAFGGPISTGKEANIYTALAPDGEVAVKVYRINASDFKQMRAYLEGDPRFEGIGSDKKKVVVAWTKKEYANLERARKAGVRVPEPIAVERNVLVMEYLGDDDRRAKRLNEVHIENPETTYEVVREYMRRLYDAGLVHGDLSEFNIVVHEGELCLLDLGQAVTVHHPNSREFLERDCENVAGFFARQGVDVDADDLLAHVTEPEPDPSGNAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUS7</accession>
    <name>A0A1U7EUS7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HNH endonuclease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1304A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUS7"/>
    <dbReference type="STRING" id="348780.NP_1304A"/>
    <dbReference type="EnsemblBacteria" id="CAI48743">
        <property type="protein sequence ID" value="CAI48743"/>
        <property type="gene ID" value="NP_1304A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_1304A"/>
    <dbReference type="eggNOG" id="arCOG03898">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1168602_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="214"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="237" mass="26186" checksum="AE9E2F3E8248EAF3" modified="2017-05-10" version="1">MELEPRADKRRTRDGQGPRWRVIREEVLERDDYTCQRCGYEQEAESGEPSKRLEAHFADAHGSPSLEQLDRLVTVCGPCHATLHGDDPAYGNRRDAAPMFPRPDAPPEVATMRSDRQHVCQRCQHVAESATELAAYTESGQAHVLCKPCAGALLAAGYDPESFEVAGALDTESLRERASEAPVRPVLLASGPVRESRPPETPTERFLYATPLRYVANPIGLTVLFILLGVVFSFAVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUU1</accession>
    <name>A0A1U7EUU1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1364A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322408.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUU1"/>
    <dbReference type="EnsemblBacteria" id="CAI48773">
        <property type="protein sequence ID" value="CAI48773"/>
        <property type="gene ID" value="NP_1364A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701127"/>
    <dbReference type="KEGG" id="nph:NP_1364A"/>
    <dbReference type="eggNOG" id="arCOG06228">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1507429_0_0_2"/>
    <dbReference type="OrthoDB" id="236176at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="178" mass="19546" checksum="215026BD6427F522" modified="2017-05-10" version="1">MGPLESLTDPAENEAVRAVAAAFAGMERDFRAAIQAHYAALGIDPPPDRPPAEERIDQLCLLVSHHFRGDLWGYFCAEQAPDALERPDDARAFAGQSDAEWEASMQQLAAAAPDDIDGSTRERAAAIIADRFGVGLDTFEREIVGWTPERTLRRALRGPMDTDIERLRRATAALEQSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUV5</accession>
    <name>A0A1U7EUV5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ArsA family ATPase</fullName>
            <ecNumber evidence="3">3.6.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">arsA2</name>
        <name evidence="3" type="synonym">tpa06</name>
        <name evidence="3" type="ordered locus">NP_1382A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arsA ATPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.-.-" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322417.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUV5"/>
    <dbReference type="STRING" id="348780.NP_1382A"/>
    <dbReference type="EnsemblBacteria" id="CAI48782">
        <property type="protein sequence ID" value="CAI48782"/>
        <property type="gene ID" value="NP_1382A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703353"/>
    <dbReference type="KEGG" id="nph:NP_1382A"/>
    <dbReference type="eggNOG" id="arCOG02849">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040761_4_0_2"/>
    <dbReference type="OrthoDB" id="46198at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02035">
        <property type="entry name" value="ArsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025723">
        <property type="entry name" value="ArsA/GET3_ATPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016300">
        <property type="entry name" value="ATPase_ArsA/GET3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00345">
        <property type="entry name" value="GET3_arsA_TRC40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803">
        <property type="entry name" value="ARSENICAL PUMP-DRIVING ATPASE ARSENITE-TRANSLOCATING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803:SF3">
        <property type="entry name" value="ATPASE GET3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02374">
        <property type="entry name" value="ArsA_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ArsA/GET3 Anion-transporting ATPase-like" evidence="2">
        <location>
            <begin position="2"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="317" mass="33763" checksum="6474E25565B7B2F7" modified="2017-05-10" version="1">MTRYVLYGGKGGVGKTTCAAATGIASGRRGEATLVVSTDPAHSLSDSFGVDVGPEPTAVAENCWAVEVDPESRMGRYRGHVSAALDELESLGITLGDDAIDDIADAGIAPGTDEAAALDLFVDYMDDPRYDRIVFDTAPTGHTLRLLELPAVLQSALGTLANVKSQMSSLADTVRGMFGTDENDDDGDSVDVDLQTLSERLERVGAALRDPERTAFRVVLVPETMAIRESERLFAELDAYGVPAGRAVVNKVIEDPTPGCERCQTQYADQQERLKTAAERFDVPIAVLPELDGEVQGLDAVETIADRLDAIGDGFTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUW9</accession>
    <name>A0A1U7EUW9_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7847 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_1372A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322412.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUW9"/>
    <dbReference type="STRING" id="348780.NP_1372A"/>
    <dbReference type="EnsemblBacteria" id="CAI48777">
        <property type="protein sequence ID" value="CAI48777"/>
        <property type="gene ID" value="NP_1372A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701130"/>
    <dbReference type="KEGG" id="nph:NP_1372A"/>
    <dbReference type="eggNOG" id="arCOG04389">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_975271_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057169">
        <property type="entry name" value="DUF7847"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25231">
        <property type="entry name" value="DUF7847"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="184"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7847" evidence="2">
        <location>
            <begin position="4"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="285" mass="29281" checksum="16EEA5404EAE7551" modified="2017-05-10" version="1">MGHALAALGDSIGTLRRTPAAAGLLGLLAIVVYSLYLGLWFVPYVGWLLSIVVFALGAGALYAVASTAIDEETASIAAAVDVIRERWLSLLGAYGMLILLFIGVLALVFVLLLVAAIAGAATMEVVDDPTAAGGFLILVAVLYVGLYLLYALVAQFVFPAVAIERTSAVDSVGTAYSVVRSAPVSVVGFTLLRVVIEYGFLILGVVGAAAVAYVSGGETLAETDPEAVDPTILAGAVDTVGIVVALGILLFGAALGQLLRITYTAAFYKRIREPGGSSAEAAEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUX8</accession>
    <name>A0A1U7EUX8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Iron-sulfur protein (4Fe-4S)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">NP_1400A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HdrC family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322426.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUX8"/>
    <dbReference type="STRING" id="348780.NP_1400A"/>
    <dbReference type="EnsemblBacteria" id="CAI48791">
        <property type="protein sequence ID" value="CAI48791"/>
        <property type="gene ID" value="NP_1400A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701137"/>
    <dbReference type="KEGG" id="nph:NP_1400A"/>
    <dbReference type="eggNOG" id="arCOG00332">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005304_1_0_2"/>
    <dbReference type="OrthoDB" id="42878at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.950.20">
        <property type="entry name" value="Transmembrane di-heme cytochromes, Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004017">
        <property type="entry name" value="Cys_rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051460">
        <property type="entry name" value="HdrC_iron-sulfur_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036197">
        <property type="entry name" value="NarG-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43255:SF1">
        <property type="entry name" value="IRON-SULFUR-BINDING OXIDOREDUCTASE FADF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43255">
        <property type="entry name" value="IRON-SULFUR-BINDING OXIDOREDUCTASE FADF-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02754">
        <property type="entry name" value="CCG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13187">
        <property type="entry name" value="Fer4_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103501">
        <property type="entry name" value="Respiratory nitrate reductase 1 gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="301"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="361"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI48791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="739" mass="82657" checksum="32EA3F07D8A73C5C" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV16</accession>
    <name>A0A1U7EV16_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1510A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322480.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV16"/>
    <dbReference type="STRING" id="348780.NP_1510A"/>
    <dbReference type="EnsemblBacteria" id="CAI48846">
        <property type="protein sequence ID" value="CAI48846"/>
        <property type="gene ID" value="NP_1510A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701164"/>
    <dbReference type="KEGG" id="nph:NP_1510A"/>
    <dbReference type="eggNOG" id="arCOG06401">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_206910_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055755">
        <property type="entry name" value="DUF7331"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24018">
        <property type="entry name" value="DUF7331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="60" mass="6400" checksum="7417D5123BC91BE0" modified="2017-05-10" version="1">MPEHNAPTEQDSTGAVEPAVADAVEATEAYETDEGVVFYDVENPLAWVQADHAVDLEAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV49</accession>
    <name>A0A1U7EV49_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glutamate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">gdhA2</name>
        <name evidence="10" type="ordered locus">NP_1582A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homohexamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 8">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322516.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV49"/>
    <dbReference type="STRING" id="348780.NP_1582A"/>
    <dbReference type="EnsemblBacteria" id="CAI48882">
        <property type="protein sequence ID" value="CAI48882"/>
        <property type="gene ID" value="NP_1582A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702749"/>
    <dbReference type="KEGG" id="nph:NP_1582A"/>
    <dbReference type="eggNOG" id="arCOG01352">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025763_1_2_2"/>
    <dbReference type="OrthoDB" id="6425at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004352">
        <property type="term" value="F:glutamate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006538">
        <property type="term" value="P:L-glutamate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01076">
        <property type="entry name" value="NAD_bind_1_Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006095">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006097">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033524">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014362">
        <property type="entry name" value="Glu_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033922">
        <property type="entry name" value="NAD_bind_Glu_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606:SF13">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02812">
        <property type="entry name" value="ELFV_dehydrog_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000185">
        <property type="entry name" value="Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00082">
        <property type="entry name" value="GLFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00074">
        <property type="entry name" value="GLFV_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal" evidence="9">
        <location>
            <begin position="183"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="351"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalysis" evidence="7">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI48882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="419" mass="45676" checksum="DA6CBBF409021AFC" modified="2017-05-10" version="1">MAEDVNPFESLQEQIDDAAEHLDVGDDVVERLKNPERVLETNLSVELDDGSIGVFRAYRSQFNGDRGPYKGGIRYHPDVDRDEVKALSGWMVYKCAVVDIPYGGGKGGIVIDPREYSTDELERITRAFAEELRPLIGEDRDIPAPDVNTGQREMNWIKDTYETLENTTAPGVITGKAIESGGSRGRVEATGRSTMLTAREAFDYLGKSLPGASVAVQGYGNAGSIAAKLLQDDHGANIVAVSDSSGAVYNPSGLDAHDVKAFKNETGSVSGYDDATEELTNEELLTLDVDLLVPAALENAIDADLAADVQADIIAEAANGPLTPAADDILTEKDVLVIPDILANAGGVTVSYFEWVQNRQRFYWTEERVNEELERHIVEAFDGLIDCYEERDLPNLRTAAYVVALRRVLSAYDEAGSWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVA9</accession>
    <name>A0A1U7EVA9_NATPD</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1716A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
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            <taxon>Halobacteria</taxon>
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            <taxon>Natronomonas</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="348780.NP_1716A"/>
    <dbReference type="EnsemblBacteria" id="CAI48949">
        <property type="protein sequence ID" value="CAI48949"/>
        <property type="gene ID" value="NP_1716A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_1716A"/>
    <dbReference type="eggNOG" id="arCOG08982">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_215620_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="43" mass="5268" checksum="17DCB63FE0983560" modified="2017-05-10" version="1">MHTVKRKTITIREDQEEWIQENHLNLSSFVQEQLDELIEQRDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVC4</accession>
    <name>A0A1U7EVC4_NATPD</name>
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        <submittedName>
            <fullName evidence="3">Peptidase M23 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_1702A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Methanobacteriota</taxon>
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            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
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            <taxon>Natronomonas</taxon>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322575.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A1U7EVC4"/>
    <dbReference type="STRING" id="348780.NP_1702A"/>
    <dbReference type="EnsemblBacteria" id="CAI48942">
        <property type="protein sequence ID" value="CAI48942"/>
        <property type="gene ID" value="NP_1702A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701230"/>
    <dbReference type="KEGG" id="nph:NP_1702A"/>
    <dbReference type="eggNOG" id="arCOG02979">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_942000_0_0_2"/>
    <dbReference type="OrthoDB" id="36515at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.70.10">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058817">
        <property type="entry name" value="DUF8155_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058468">
        <property type="entry name" value="DUF8155_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011055">
        <property type="entry name" value="Dup_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26482">
        <property type="entry name" value="DUF8155"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8155" evidence="1">
        <location>
            <begin position="3"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="DUF8155" evidence="2">
        <location>
            <begin position="144"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="272" mass="28805" checksum="F15ED14D81D4A4A0" modified="2017-05-10" version="1">MVTLPAAALDPYERFSRYNSPYPAHDDGCAVDLYPARKTAYAPVSGEVRSIRTVQCPEKPYGTSVDHLVCVDCGDHIARLLHVDPAVEVGEHIEVGDRIGTLIRSGFFARWVDHHLHVGFRDHGQNIERASGSLPLTVDVPVEGVGWSGYGTVIEAGQTYVVLDAPGGNEEGFVALTADDGTPLDGGLPHYDRGGAFGAFPKTVSLLGTEVGHEDGRGITWCDVEIRASGRAATGLSLFASRGSVSVKLVFGSGHDVAVGDELTIAIEPSEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVC9</accession>
    <name>A0A1U7EVC9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HAD superfamily hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1762A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVC9"/>
    <dbReference type="STRING" id="348780.NP_1762A"/>
    <dbReference type="EnsemblBacteria" id="CAI48972">
        <property type="protein sequence ID" value="CAI48972"/>
        <property type="gene ID" value="NP_1762A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701248"/>
    <dbReference type="KEGG" id="nph:NP_1762A"/>
    <dbReference type="eggNOG" id="arCOG02292">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1154322_0_0_2"/>
    <dbReference type="OrthoDB" id="31229at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008967">
        <property type="term" value="F:phosphoglycolate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050155">
        <property type="entry name" value="HAD-like_hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43434">
        <property type="entry name" value="PHOSPHOGLYCOLATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43434:SF1">
        <property type="entry name" value="PHOSPHOGLYCOLATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="207" mass="22574" checksum="CA11F47A1050BD8B" modified="2017-05-10" version="1">MTDSTYDRWVFDLDGTLVDVEPEYVRSVFDRVGDRLGYAFSERQADGIWHGLGGARNEALRSWGVAPEQFWTAFHEVEDPAARADATFLYADAAVVGDIDAPTALVTHCQSYLTEAVLSELDIEDWFDTVVCCTESTGWKPDPKPVELALSALDADGNGVLVGDGPHDIGAAWNAGLDGAHVERHSPEQRGLCVVGDHRLSRVDELA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVE1</accession>
    <name>A0A1U7EVE1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1784A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322616.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVE1"/>
    <dbReference type="EnsemblBacteria" id="CAI48983">
        <property type="protein sequence ID" value="CAI48983"/>
        <property type="gene ID" value="NP_1784A"/>
    </dbReference>
    <dbReference type="GeneID" id="54763312"/>
    <dbReference type="KEGG" id="nph:NP_1784A"/>
    <dbReference type="eggNOG" id="arCOG14540">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3194603_0_0_2"/>
    <dbReference type="OrthoDB" id="226402at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="47" mass="5213" checksum="F07456EE634493FC" modified="2017-05-10" version="1">MPRDEIEPMVPILPTDEQTETTARTDGGHQQRYHNGDVDAPLVPNLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVG1</accession>
    <name>A0A1U7EVG1_NATPD</name>
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        <submittedName>
            <fullName evidence="3">IS1341-type transposase ISNph24</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_1856A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322651.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVG1"/>
    <dbReference type="SMR" id="A0A1U7EVG1"/>
    <dbReference type="EnsemblBacteria" id="CAI49019">
        <property type="protein sequence ID" value="CAI49019"/>
        <property type="gene ID" value="NP_1856A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701278"/>
    <dbReference type="KEGG" id="nph:NP_1856A"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_3_1_2"/>
    <dbReference type="OrthoDB" id="168528at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas12f1-like TNB" evidence="2">
        <location>
            <begin position="277"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="350" mass="40187" checksum="3B6C4B99FFF520D4" modified="2017-05-10" version="1">MVEVSVETTFRNPSRSRRQEWQRATHILRECKQWLVDGWEDDSLASSVTTGDIDNPLYSALQNQAIRNAKSDYADESIEYTGEQPIEVNNQNWEIHTTENDSVVIGFPCISDWWYTPIHVHESIEEPVQALLDGDAEKTKLSVWREGGDWHCSFTVEYDEQQDGEETPIGVDVGHNYILAATPDNASAESFLVSGKEHKFVRRYYRSLRDSLQETGAHRARTRVGNKEYRRIQDMNHTLSKRLVEYASQFENPVIKLEDLENIRDGSEWNGVHSWPFYELQQFITYKAEKEGVCVEKVDPENTSQECSQCDELVGRDGSKFECPHCGYVRHADLNAAENISQREGDPCTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVI2</accession>
    <name>A0A1U7EVI2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Thiamine pyrophosphate-dependent enzyme (Homolog to acetolactate synthase / benzoylformate decarboxylase)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ilvB2</name>
        <name evidence="8" type="synonym">mdlC</name>
        <name evidence="8" type="ordered locus">NP_1904A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the TPP enzyme family.</text>
    </comment>
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        <property type="protein sequence ID" value="CAI49043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322675.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVI2"/>
    <dbReference type="STRING" id="348780.NP_1904A"/>
    <dbReference type="EnsemblBacteria" id="CAI49043">
        <property type="protein sequence ID" value="CAI49043"/>
        <property type="gene ID" value="NP_1904A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702866"/>
    <dbReference type="KEGG" id="nph:NP_1904A"/>
    <dbReference type="eggNOG" id="arCOG03656">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013748_3_1_2"/>
    <dbReference type="OrthoDB" id="6837at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02002">
        <property type="entry name" value="TPP_BFDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07035">
        <property type="entry name" value="TPP_PYR_POX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="THIAMINE PYROPHOSPHATE ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="7">
        <location>
            <begin position="6"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="5">
        <location>
            <begin position="201"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="6">
        <location>
            <begin position="426"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="362"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="564" mass="60231" checksum="7CB8146EBB25D41A" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVI7</accession>
    <name>A0A1U7EVI7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">tRNA (Guanine(37)-N(1))-methyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.228</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">trm5</name>
        <name evidence="7" type="ordered locus">NP_1886A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.228" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322666.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVI7"/>
    <dbReference type="STRING" id="348780.NP_1886A"/>
    <dbReference type="EnsemblBacteria" id="CAI49034">
        <property type="protein sequence ID" value="CAI49034"/>
        <property type="gene ID" value="NP_1886A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701291"/>
    <dbReference type="KEGG" id="nph:NP_1886A"/>
    <dbReference type="eggNOG" id="arCOG00033">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022610_0_1_2"/>
    <dbReference type="OrthoDB" id="8079at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052906">
        <property type="term" value="F:tRNA (guanine(37)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002939">
        <property type="term" value="P:tRNA N1-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.110">
        <property type="entry name" value="Met-10+ protein-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030382">
        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056743">
        <property type="entry name" value="TRM5-TYW2-like_MTfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056744">
        <property type="entry name" value="TRM5/TYW2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040601">
        <property type="entry name" value="Trm5a/b_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245:SF36">
        <property type="entry name" value="TRNA (GUANINE(37)-N1)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245">
        <property type="entry name" value="TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="TRM5-TYW2_MTfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18093">
        <property type="entry name" value="Trm5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25133">
        <property type="entry name" value="TYW2_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51684">
        <property type="entry name" value="SAM_MT_TRM5_TYW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SAM-dependent methyltransferase TRM5/TYW2-type" evidence="6">
        <location>
            <begin position="87"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="345" mass="38361" checksum="C34796701FB89594" modified="2017-05-10" version="1">MSVRCVRVPREDGETARGRIADADALADDYEIELDDGHLYLPIVESADEAALEAALSDLDADIVDRRPAARETQTLPADILGFEPSYERLGDIAIIDEDDPERARRVANAIADSELPIETVVNRASPVEGELRVREWDVLVGDTTETVHREYGCAFEVDIAEVYFSPRLATERHRIVSDVEADESVFDMFAGVGPFVIPAAKRGADCVGVDLNAAAIEYLRRNAERNSVADRITAIHGDVREVVSEPPDERSEAADSYDDWADRIVMNLPHSADEFLDTAVRLAGNDCLLHYYDIQHDSDPFGPGERAIREAAAPEYDVDIETRREVRSYAPHELNVCLDVRLTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVL0</accession>
    <name>A0A1U7EVL0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphate transport system permease protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">pstC1</name>
        <name evidence="8" type="synonym">abc14p2</name>
        <name evidence="8" type="ordered locus">NP_1980A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Part of the binding-protein-dependent transport system for phosphate; probably responsible for the translocation of the substrate across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322711.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVL0"/>
    <dbReference type="STRING" id="348780.NP_1980A"/>
    <dbReference type="EnsemblBacteria" id="CAI49081">
        <property type="protein sequence ID" value="CAI49081"/>
        <property type="gene ID" value="NP_1980A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702413"/>
    <dbReference type="KEGG" id="nph:NP_1980A"/>
    <dbReference type="eggNOG" id="arCOG00167">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033621_1_0_2"/>
    <dbReference type="OrthoDB" id="338493at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011864">
        <property type="entry name" value="Phosphate_PstC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02138">
        <property type="entry name" value="phosphate_pstC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42727:SF1">
        <property type="entry name" value="PHOSPHATE TRANSPORT SYSTEM PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42727">
        <property type="entry name" value="PHOSPHATE TRANSPORT SYSTEM PERMEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="77"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="115"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="267"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="7">
        <location>
            <begin position="78"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="302" mass="32352" checksum="9D820A3984107C0B" modified="2017-05-10" version="1">MSTTDITDDAVSDRATTIARVVFFACALVTVLTTFGIVLVLITGSIPFFREVSVIEFFTSTEWSPRIEPNSYGVLPLVWGTMMIVVGSAAIALPVGVLTAIYLSEYADRRTRKVLKPTLEILAGVPTIVYGFFAISFITPQIQRVFPETGTFNALSAAIVVGIMIIPMVSSLSEDAMSSVPDDLRNAGYGLGATKFEVSTQIVLPAAVSGILASFVLAISRAIGETMAVTLAAGMSPTITLDPLEEIQTMTAYMVEIGIGDVSVGSIGYQSLFAVGLLLFVMTLAMNVLSQLVRSRYREVYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVN7</accession>
    <name>A0A1U7EVN7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Triphosphoribosyl-dephospho-CoA synthase</fullName>
            <ecNumber evidence="2">2.4.2.52</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">citG</name>
        <name evidence="2" type="ordered locus">NP_2024A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.4.2.52" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322732.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVN7"/>
    <dbReference type="STRING" id="348780.NP_2024A"/>
    <dbReference type="EnsemblBacteria" id="CAI49103">
        <property type="protein sequence ID" value="CAI49103"/>
        <property type="gene ID" value="NP_2024A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701329"/>
    <dbReference type="KEGG" id="nph:NP_2024A"/>
    <dbReference type="eggNOG" id="arCOG04238">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063627_0_0_2"/>
    <dbReference type="OrthoDB" id="85890at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046917">
        <property type="term" value="F:triphosphoribosyl-dephospho-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4200.10">
        <property type="entry name" value="Triphosphoribosyl-dephospho-CoA protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002736">
        <property type="entry name" value="CitG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42280">
        <property type="entry name" value="CITG FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42280:SF1">
        <property type="entry name" value="CITG FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01874">
        <property type="entry name" value="CitG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="291" mass="30602" checksum="6C25C4D079B5FE4E" modified="2017-05-10" version="1">MAEESTDSRPAAPRRGPAQNAQLALLLEVAGTPKPGNVDRRRDYDDLRLEHFMAGAVGAMGGLAAAADTTPGVGECFERAVAGMAEQSAGNTQFGALLLLVPLVRAAATSRLTPAGTRTVVESTTVEDACGFYRAFEHVDVAVDDPPADADALDVRRGGDAAPELRERALTLADVMALSADRDGVAAELTGGFERSFTAASWLSDDDGPIYQRASRTFLRLLADEPDTFVVTRNGRAAAEEATARAQAVLEGREDADDLAAEFVDRDINPGTTADITAAALFIALQRGLEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVR8</accession>
    <name>A0A1U7EVR8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Taxis cluster protein CheC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cheC1</name>
        <name evidence="4" type="ordered locus">NP_2104A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322771.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVR8"/>
    <dbReference type="STRING" id="348780.NP_2104A"/>
    <dbReference type="EnsemblBacteria" id="CAI49143">
        <property type="protein sequence ID" value="CAI49143"/>
        <property type="gene ID" value="NP_2104A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702599"/>
    <dbReference type="KEGG" id="nph:NP_2104A"/>
    <dbReference type="eggNOG" id="arCOG02381">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_654911_0_0_2"/>
    <dbReference type="OrthoDB" id="182374at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17911">
        <property type="entry name" value="CheC_ClassIII"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1550.10">
        <property type="entry name" value="CheC-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007597">
        <property type="entry name" value="CheC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028976">
        <property type="entry name" value="CheC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050992">
        <property type="entry name" value="CheZ_family_phosphatases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43693">
        <property type="entry name" value="PROTEIN PHOSPHATASE CHEZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43693:SF1">
        <property type="entry name" value="PROTEIN PHOSPHATASE CHEZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04509">
        <property type="entry name" value="CheC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103039">
        <property type="entry name" value="CheC-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CheC-like protein" evidence="3">
        <location>
            <begin position="98"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="CheC-like protein" evidence="3">
        <location>
            <begin position="289"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="398" mass="43225" checksum="359837C03B2C0DB8" modified="2017-05-10" version="1">MQIDIDSLETYNTLARDGAQSAAQSLSQLTGIDTHVEVTNVSLMDPSDLEYEYIGGEFSGVEIGLEGALSGETVLTFDEAAQETITNVLAPGADEAMRRSSIKEVGNIMTSGFIDGWADHLDAQIDISTPTYLEGTGSDVLPASAADSDEQVFVFRSRVEAVSEAVDFHILLVPERDTLVDALDPDEDDSIPFEKFEVFNEMTMHGAEKAAENITTMTGLTTDVEVNRMTFVPIDDVPAEVGDRRYVGTVMEYEGTPSGYLVILFDQSSASSVVDALVPVETDGDWGEMEQSAMKELGNIMTSGFIDGWANVLQTSIDHSPPTFVADMGSSIMSPIVGRMARTQEHAFMLDSVINTDGDGVFRCEMFALPDEAELTDVLDDLLVERADETEVEPDEVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVT9</accession>
    <name>A0A1U7EVT9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Flagellar motor/biogenesis protein FlaI</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">flaI</name>
        <name evidence="4" type="ordered locus">NP_2158A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GSP E family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322798.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVT9"/>
    <dbReference type="STRING" id="348780.NP_2158A"/>
    <dbReference type="EnsemblBacteria" id="CAI49170">
        <property type="protein sequence ID" value="CAI49170"/>
        <property type="gene ID" value="NP_2158A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702715"/>
    <dbReference type="KEGG" id="nph:NP_2158A"/>
    <dbReference type="eggNOG" id="arCOG01817">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005379_2_2_2"/>
    <dbReference type="OrthoDB" id="33500at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01130">
        <property type="entry name" value="VirB11-like_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050921">
        <property type="entry name" value="T4SS_GSP_E_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30486:SF14">
        <property type="entry name" value="FLAGELLA ACCESSORY PROTEIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30486">
        <property type="entry name" value="TWITCHING MOTILITY PROTEIN PILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="4" id="KW-0282">Flagellum</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bacterial type II secretion system protein E" evidence="3">
        <location>
            <begin position="231"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="557" mass="63257" checksum="C170F3D556A6C84B" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVY4</accession>
    <name>A0A1U7EVY4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">APH family phosphotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2224A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322830.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVY4"/>
    <dbReference type="STRING" id="348780.NP_2224A"/>
    <dbReference type="EnsemblBacteria" id="CAI49203">
        <property type="protein sequence ID" value="CAI49203"/>
        <property type="gene ID" value="NP_2224A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701382"/>
    <dbReference type="KEGG" id="nph:NP_2224A"/>
    <dbReference type="eggNOG" id="arCOG04682">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007526_0_0_2"/>
    <dbReference type="OrthoDB" id="350437at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05154">
        <property type="entry name" value="ACAD10_11_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052898">
        <property type="entry name" value="ACAD10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041726">
        <property type="entry name" value="ACAD10_11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47829:SF1">
        <property type="entry name" value="HAD FAMILY PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47829">
        <property type="entry name" value="HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G12880)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoglycoside phosphotransferase" evidence="1">
        <location>
            <begin position="34"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="360" mass="41632" checksum="7AA99EE3AFDC28B1" modified="2017-05-10" version="1">MTDTDPEYFERIVDEEALRAYLTDALGPADGFDVRHHQEGHSNETLFVTWGDRELVIRRPPPGDTAENAHDVLREYRVVDALQDTDVRVPTTVVACDDHSVIGSDFYAMEKEAGDVLRDAEPDRFANPAAREQIGYELVDRLVEIHEVDYEAVGLEEGDFGYPPGFTERQVRRWSEQLTWAFEVTSDEREVNELYDVMEWLYDNVPGDDEYPNTLVHGDYKLDNVMFAPKDEPEIAAIFDWEMATLGDPFTDLGWMLSYWRQPKDPEPPTPSLTQTFMTEDGYPTRRELVDRYEQQTGFEFDNWRFYWVLATYKLAGLGEMFFRRYLEGNSADPMYPKMEHGVPALAEQALDIIDGEMTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW09</accession>
    <name>A0A1U7EW09_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Methylmalonyl-CoA mutase subunit A</fullName>
            <ecNumber evidence="4">5.4.99.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">mmcA1</name>
        <name evidence="4" type="ordered locus">NP_2320A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="5.4.99.2" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322878.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW09"/>
    <dbReference type="STRING" id="348780.NP_2320A"/>
    <dbReference type="EnsemblBacteria" id="CAI49251">
        <property type="protein sequence ID" value="CAI49251"/>
        <property type="gene ID" value="NP_2320A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702900"/>
    <dbReference type="KEGG" id="nph:NP_2320A"/>
    <dbReference type="eggNOG" id="arCOG04232">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009523_5_1_2"/>
    <dbReference type="OrthoDB" id="38408at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004494">
        <property type="term" value="F:methylmalonyl-CoA mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd03680">
        <property type="entry name" value="MM_CoA_mutase_ICM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.240">
        <property type="entry name" value="Methylmalonyl-CoA mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016176">
        <property type="entry name" value="Cbl-dep_enz_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006099">
        <property type="entry name" value="MeMalonylCoA_mutase_a/b_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006098">
        <property type="entry name" value="MMCoA_mutase_a_cat"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00641">
        <property type="entry name" value="acid_CoA_mut_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48101:SF1">
        <property type="entry name" value="METHYLMALONYL-COA MUTASE, LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48101">
        <property type="entry name" value="METHYLMALONYL-COA MUTASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01642">
        <property type="entry name" value="MM_CoA_mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51703">
        <property type="entry name" value="Cobalamin (vitamin B12)-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methylmalonyl-CoA mutase alpha/beta chain catalytic" evidence="3">
        <location>
            <begin position="33"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="561" mass="62364" checksum="1D6E9B89C12A60BB" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW44</accession>
    <name>A0A1U7EW44_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2426A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322930.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW44"/>
    <dbReference type="STRING" id="348780.NP_2426A"/>
    <dbReference type="EnsemblBacteria" id="CAI49304">
        <property type="protein sequence ID" value="CAI49304"/>
        <property type="gene ID" value="NP_2426A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701440"/>
    <dbReference type="KEGG" id="nph:NP_2426A"/>
    <dbReference type="eggNOG" id="arCOG11104">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1881139_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="135" mass="13969" checksum="C4BDBA27522997B6" modified="2017-05-10" version="1">MSDEDEYRFHPEDFEGDSDPTDETASVAPLVIVVCLGVGILLFLAGTVTDGLPVLGVEVDLVALSAAVFAVGLFAGSVMYLRQGRRVVGAVHGLGALGWTFLVFGTLLSEAVLLAAGVAVLVVGSVALLVISIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW46</accession>
    <name>A0A1U7EW46_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2360A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322897.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW46"/>
    <dbReference type="STRING" id="348780.NP_2360A"/>
    <dbReference type="EnsemblBacteria" id="CAI49271">
        <property type="protein sequence ID" value="CAI49271"/>
        <property type="gene ID" value="NP_2360A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701421"/>
    <dbReference type="KEGG" id="nph:NP_2360A"/>
    <dbReference type="HOGENOM" id="CLU_487160_0_0_2"/>
    <dbReference type="OrthoDB" id="384059at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="559" mass="62673" checksum="7D4692F6773BB769" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW76</accession>
    <name>A0A1U7EW76_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Isocitrate dehydrogenase (NADP(+))</fullName>
            <ecNumber evidence="4 13">1.1.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">icd</name>
        <name evidence="20" type="ordered locus">NP_2430A</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>D-threo-isocitrate + NADP(+) = 2-oxoglutarate + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:19629"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="16">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="16">Binds 1 Mg(2+) or Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.42" evidence="4 13"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322932.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW76"/>
    <dbReference type="STRING" id="348780.NP_2430A"/>
    <dbReference type="EnsemblBacteria" id="CAI49306">
        <property type="protein sequence ID" value="CAI49306"/>
        <property type="gene ID" value="NP_2430A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702856"/>
    <dbReference type="KEGG" id="nph:NP_2430A"/>
    <dbReference type="eggNOG" id="arCOG01164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031953_7_1_2"/>
    <dbReference type="OrthoDB" id="23624at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004450">
        <property type="term" value="F:isocitrate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006097">
        <property type="term" value="P:glyoxylate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004439">
        <property type="entry name" value="Isocitrate_DH_NADP_dimer_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005425">
        <property type="entry name" value="PRK07006.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00183">
        <property type="entry name" value="prok_nadp_idh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43504">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NADP]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43504:SF1">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NADP]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0329">Glyoxylate bypass</keyword>
    <keyword evidence="8 16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 16" id="KW-0464">Manganese</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 15" id="KW-0521">NADP</keyword>
    <keyword evidence="10 20" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="19">
        <location>
            <begin position="30"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="337"/>
            <end position="343"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="350"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="389"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="393"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="site" description="Critical for catalysis" evidence="17">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <feature type="site" description="Critical for catalysis" evidence="17">
        <location>
            <position position="227"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-succinyllysine" evidence="18">
        <location>
            <position position="97"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="18">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-acetyllysine" evidence="18">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="NCBIfam" id="TIGR00183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR604439-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR604439-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR604439-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR604439-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR604439-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAI49306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="418" mass="46218" checksum="D5F35F2919DCAD51" modified="2017-05-10" version="1">MGYDKVEVPAEGEKVAVDEDDELIVPDDPVIPIIHGDGIGTDVGPAAQKVLQAAADATGREINWMRVYAGESAREKYDENLPEDTIEALKEFKVSIKGPLTTPVGAGFRSLNVALRKKLDLYTNIRPTYHLDGVPSPVKQPEQMDMVTFRENTEDVYAGIEWEAGTDEVQEVKEFVEEQMGYDDVIHDGEVGIGVKPITEFGTKRLVRQAIDYALEKDRDSVTLVHKGNIMKFTEGAFRDWGYEVAREEYGEEVITEDTLWEERDGEPPEDAVVVNDRIADNMLQQILTRTDQYDVLAMPNLNGDYLSDACGAQIGGLGIAPGANIGDGRVLAEPVHGSAPKYAGQDKVNPTAMILSGRIMLEFMGWDDAADLVRDAVEQTIVDRKVTYDIERQVEDAEKLATSEYAEAVVDNLYDLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW86</accession>
    <name>A0A1U7EW86_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cox cluster protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2452A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322943.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW86"/>
    <dbReference type="STRING" id="348780.NP_2452A"/>
    <dbReference type="EnsemblBacteria" id="CAI49317">
        <property type="protein sequence ID" value="CAI49317"/>
        <property type="gene ID" value="NP_2452A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701447"/>
    <dbReference type="KEGG" id="nph:NP_2452A"/>
    <dbReference type="eggNOG" id="arCOG04643">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155016_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055963">
        <property type="entry name" value="DUF7541"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24396">
        <property type="entry name" value="DUF7541"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="136" mass="14005" checksum="69721C042E8D351B" modified="2017-05-10" version="1">MGTESDSERMADRYDKTSPWPLVIVLGLVLSEIGILFNVYPVAIAGLVMFVGSVSGIISEAGYVASPWRLVSGLGLFLAVVGALVIWTQLDAVSVDAAATAVAVPANDIVQRGVTIAATGGVLAIGGVVLPRLRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWA2</accession>
    <name>A0A1U7EWA2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cytochrome P450</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cyc</name>
        <name evidence="8" type="ordered locus">NP_2540A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322986.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWA2"/>
    <dbReference type="STRING" id="348780.NP_2540A"/>
    <dbReference type="EnsemblBacteria" id="CAI49361">
        <property type="protein sequence ID" value="CAI49361"/>
        <property type="gene ID" value="NP_2540A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702648"/>
    <dbReference type="KEGG" id="nph:NP_2540A"/>
    <dbReference type="eggNOG" id="arCOG02814">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_5_1_2"/>
    <dbReference type="OrthoDB" id="9881at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF50">
        <property type="entry name" value="BIFUNCTIONAL ALBAFLAVENONE MONOOXYGENASE_TERPENE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0349">Heme</keyword>
    <keyword evidence="5 7" id="KW-0408">Iron</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="448" mass="49572" checksum="3E90897CB930E19D" modified="2017-05-10" version="1">MSTRLPEPPEAGLLNALRFGTDTFRFLEGIQARFDDGTSVSIPGRPPLVVLTGPDLVGEALDRPEDFPRVPAQDAVTMIAENGLVQSEGALWSQQRSVMAPSFSGEQVTAYANTTGRRIEARADQWAAAGARTTDLHREMTSLTVRVASEILLGEDIGTERADQFHEWMQVAGEEFEFGIETVLPDWVPTPTSGEFEQAAAGIRELSEQLIERRRESLAAGERPDASDMLTMLIRAEDNPDIDYPENQIRDEVATFLIAGHETTALSLTYTLCLLSWHPEARRRVRQEADEALGDGPPTHDDLAELTYTRRVYDEALRLYPPAWGVFRQANGDVTLGQYTIPDGSAVIMPLWSIHRDGSYFEQPDTFDPDRWERRTPRAVDAYRPFSSGPHACIGRGFALAGSTLVLARLVRDFDIDVPESALDDLRLTPTLRPADGVEATIQPVGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWB2</accession>
    <name>A0A1U7EWB2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fumarylacetoacetase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hpcE3</name>
        <name evidence="4" type="ordered locus">NP_2562A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322997.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWB2"/>
    <dbReference type="STRING" id="348780.NP_2562A"/>
    <dbReference type="EnsemblBacteria" id="CAI49372">
        <property type="protein sequence ID" value="CAI49372"/>
        <property type="gene ID" value="NP_2562A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702799"/>
    <dbReference type="KEGG" id="nph:NP_2562A"/>
    <dbReference type="eggNOG" id="arCOG00235">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028458_4_2_2"/>
    <dbReference type="OrthoDB" id="6242at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018773">
        <property type="term" value="F:acetylpyruvate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="entry name" value="Fumarylacetoacetase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11820">
        <property type="entry name" value="ACYLPYRUVASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11820:SF7">
        <property type="entry name" value="ACYLPYRUVASE FAHD1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="FAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarylacetoacetase-like C-terminal" evidence="3">
        <location>
            <begin position="46"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="236" mass="26308" checksum="42C02319AA0CA63B" modified="2017-05-10" version="1">MKFARIDTDDGVFEGEYDDGTVHTDEGSYEPAEYELLAPCEPSVFYCVGRNFGEKVDQMDYEVPEVPDFFIKPPVSLHDPETPIPYPSFSEELTYAGELAAVIDTECKNVAEDEVDDVVRGYTILNDLDCLDQERRTARKAFDSSGPLGPVVADIDPVGLDMTTHINGELRQEDNTENMFIKPREVISFLSERFTLKPDDVISFGSPANPGLLERGDEIEIYYEGIGTLRNTVGGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWB5</accession>
    <name>A0A1U7EWB5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein-export membrane protein SecF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 11" type="primary">secF</name>
        <name evidence="11" type="ordered locus">NP_2524A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in protein export.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Part of the protein translocation apparatus. Forms a complex with SecD.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SecD/SecF family. SecF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939537.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWB5"/>
    <dbReference type="STRING" id="348780.NP_2524A"/>
    <dbReference type="EnsemblBacteria" id="CAI49353">
        <property type="protein sequence ID" value="CAI49353"/>
        <property type="gene ID" value="NP_2524A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703202"/>
    <dbReference type="KEGG" id="nph:NP_2524A"/>
    <dbReference type="eggNOG" id="arCOG03054">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060478_0_0_2"/>
    <dbReference type="OrthoDB" id="85411at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1640.10">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01464_A">
        <property type="entry name" value="SecF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022813">
        <property type="entry name" value="SecD/SecF_arch_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053476">
        <property type="entry name" value="SecD/SecF_export"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048634">
        <property type="entry name" value="SecD_SecF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024921">
        <property type="entry name" value="SecF_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006355">
        <property type="entry name" value="PRK08578.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041305">
        <property type="entry name" value="SecF_Halo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30081:SF8">
        <property type="entry name" value="PROTEIN TRANSLOCASE SUBUNIT SECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30081">
        <property type="entry name" value="PROTEIN-EXPORT MEMBRANE PROTEIN SEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02355">
        <property type="entry name" value="SecD_SecF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82866">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="226"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="256"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="Protein export membrane protein SecD/SecF C-terminal" evidence="10">
        <location>
            <begin position="121"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI49353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="289" mass="31007" checksum="D0B197276026BCEB" modified="2017-05-10" version="1">MDLSVPKVDYSDYTNRQLVTIPLAVLAFALAILAVATVVTGSPVALGMDFTGGAELQVETTDSQAEIEAQFDEPVESITQVGSPTSNTYIIEFQSGDYDDLGDLVAQAEAAGYEIQQEGERSASFAADSQQQALFGIAIAFAGMALLVAAMFRTFVPSIAVIASAFSDIMIPLALMRLFDIELTLGTVAALLMLIGYSVDSDLLLNNHVLRRHGDFYTSTFRAMRTGVTMTVTSIAAMIVMTVVASLLGIPLLPDVGLILVFGLLADLMNTYLLNVSLLRYYKYEGIAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWC0</accession>
    <name>A0A1U7EWC0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Pyridoxal phosphate-dependent aminotransferase</fullName>
            <ecNumber evidence="10">2.6.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">NP_2578A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="10"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323005.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWC0"/>
    <dbReference type="STRING" id="348780.NP_2578A"/>
    <dbReference type="EnsemblBacteria" id="CAI49380">
        <property type="protein sequence ID" value="CAI49380"/>
        <property type="gene ID" value="NP_2578A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702494"/>
    <dbReference type="KEGG" id="nph:NP_2578A"/>
    <dbReference type="eggNOG" id="arCOG00082">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027686_0_0_2"/>
    <dbReference type="OrthoDB" id="166535at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008453">
        <property type="term" value="F:alanine-glyoxylate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004760">
        <property type="term" value="F:L-serine-pyruvate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019265">
        <property type="term" value="P:glycine biosynthetic process, by transamination of glyoxylate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000027">
        <property type="entry name" value="Serine--pyruvate aminotransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024169">
        <property type="entry name" value="SP_NH2Trfase/AEP_transaminase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21152:SF40">
        <property type="entry name" value="ALANINE--GLYOXYLATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21152">
        <property type="entry name" value="AMINOTRANSFERASE CLASS V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000524">
        <property type="entry name" value="SPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="9">
        <location>
            <begin position="39"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI49380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="398" mass="42170" checksum="5CC15FD32AF80787" modified="2017-05-10" version="1">MDSIDVDELRPPERLLMGPGPSETHPRVLAAFGTPPVGHLDPGFVEMMDETQELLRATLGTENEWTIPVSGTGSAAMEAAIANLVAPGDTAVVPGNGYFGDRMAEMVRRAGGEVAVVETAWGEPLDPAAVRDACATHDPDVVGVVHAETSTGVLQPSMEEITAAAHDHGALVVADCVTSLGGVEFRMDEWGVDVAYSAGQKCLSCPPGASPLSVNDAAMEKILDRDEAVRSWYLDLSLLDEYWGDERAYHHTAPVNNVYALREALRLVQEEGLEARWQRHRRFSAALQAGLWELGLEPVVDENARLPSLTTARLPDGLTPGAVVATMRDDHNVEIATGLGAFADEAVRIGCMGHGARRGPILETLGALGETLVAHGVDVDPGTGVAAATAERSADEKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWC6</accession>
    <name>A0A1U7EWC6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">UPF0219 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_2608A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323020.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWC6"/>
    <dbReference type="STRING" id="348780.NP_2608A"/>
    <dbReference type="EnsemblBacteria" id="CAI49395">
        <property type="protein sequence ID" value="CAI49395"/>
        <property type="gene ID" value="NP_2608A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702957"/>
    <dbReference type="KEGG" id="nph:NP_2608A"/>
    <dbReference type="eggNOG" id="arCOG01767">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039592_7_1_2"/>
    <dbReference type="OrthoDB" id="9573at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002878">
        <property type="entry name" value="ChsH2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022002">
        <property type="entry name" value="ChsH2_Znr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052513">
        <property type="entry name" value="Thioester_dehydratase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34075">
        <property type="entry name" value="BLR3430 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34075:SF5">
        <property type="entry name" value="BLR3430 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01796">
        <property type="entry name" value="OB_ChsH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12172">
        <property type="entry name" value="zf-ChsH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ChsH2 rubredoxin-like zinc ribbon" evidence="3">
        <location>
            <begin position="344"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="ChsH2 C-terminal OB-fold" evidence="2">
        <location>
            <begin position="381"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="476" mass="48356" checksum="9DA044EB6020337D" modified="2017-05-10" version="1">MTGIAGVGAYAPRFRITAAAFEDAWGRFEAAGVEEKAVPAADEDALTMGYEAATRALDAAAVDATDIDWLGVASSRPPIAEEDVSARLGAMLGLSDAATRHVFTGSTRAGTRALWACLDAVDAGTATGLVVAADCPAGTPDDGIDHAAGAGAAAFVVGPDGGARIVDRAEYAAPYPGTRFRRTGEAETRGLGITEYDRQAFTEAVGAAADRLDAIDDPAAAAIQAPDGKLPYRAAGAAGVDAETIQAASTVHELGDLGAASVPVSLAKGLADGRESLLAVSYGSGAGADAFAVAADGDVPTRMALDGDASLSYAEYLRQRGVVTAGPPSGGGAYVSVPSWRRSLPQRYRLVAGRCAECGALSFPPEGACDDCNGLVAYDPVELSGEGTIEAVTTISQGGAPPEFAEQQDRAGDYAAAVVALDAPSGARSVSVPAMGTDAAPADFDVGDRVETTVRRIYTQEGVTRYGFKVRPMADK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWC7</accession>
    <name>A0A1U7EWC7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Stomatin family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_2594A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323013.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWC7"/>
    <dbReference type="STRING" id="348780.NP_2594A"/>
    <dbReference type="EnsemblBacteria" id="CAI49388">
        <property type="protein sequence ID" value="CAI49388"/>
        <property type="gene ID" value="NP_2594A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701501"/>
    <dbReference type="KEGG" id="nph:NP_2594A"/>
    <dbReference type="eggNOG" id="arCOG01915">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047969_3_0_2"/>
    <dbReference type="OrthoDB" id="141114at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03401">
        <property type="entry name" value="SPFH_prohibitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000163">
        <property type="entry name" value="Prohibitin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42911">
        <property type="entry name" value="MODULATOR OF FTSH PROTEASE HFLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42911:SF2">
        <property type="entry name" value="PROHIBITIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Band 7" evidence="4">
        <location>
            <begin position="34"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="273"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="204"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="280"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="295" mass="32719" checksum="390D0F560402FC4D" modified="2017-05-10" version="1">MADDILQEQFGPRLQAAAVAGIVLLLVVGIAFLFSVATVDEGDRGVKKVQGSVTGDVLEPGWHFPLVPFYHSVEYIEIRPQTYTMSGDVFEGDVAEEDAVDFRSADQQRVGADITVRYRVNEDGADEFHREWNTIDQYEQRLLRPETVDTVAREASALNATEANSDEGRELLGDIIADELRSQSPRYVDIESVQVRDIHFDPEFEQALEQVEIAQQEADAERTRAQGDADAERIRAEGEADALREVQEALTEENLALEQIRAYDEGTVYVTDGGTPVILDPEEGSLDDEGVTADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWC8</accession>
    <name>A0A1U7EWC8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">UspA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2596A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323014.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWC8"/>
    <dbReference type="STRING" id="348780.NP_2596A"/>
    <dbReference type="EnsemblBacteria" id="CAI49389">
        <property type="protein sequence ID" value="CAI49389"/>
        <property type="gene ID" value="NP_2596A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701502"/>
    <dbReference type="KEGG" id="nph:NP_2596A"/>
    <dbReference type="eggNOG" id="arCOG06441">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1754636_0_0_2"/>
    <dbReference type="OrthoDB" id="260697at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="133" mass="15031" checksum="E5C5AC06A0A20005" modified="2017-05-10" version="1">MTLVVAPVRYPLSPNSKVTLQTAIQVADDHDADLTVLHVNLYQNDRRVTRTELKNAVEAELGTVDRARYVVRPGFLVEETILDEVAAEDADYVVIGENQTSRWRRLVSRLVDDPNIEQFLRNELDCEVITAAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWF4</accession>
    <name>A0A1U7EWF4_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10 11">Methenyltetrahydromethanopterin cyclohydrolase</fullName>
            <ecNumber evidence="3 10 11">3.5.4.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 10">Methenyl-H4MPT cyclohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10 12" type="primary">mch</name>
        <name evidence="12" type="ordered locus">NP_2634A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the hydrolysis of methenyl-H(4)MPT(+) to 5-formyl-H(4)MPT.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O = N(5)-formyl-5,6,7,8-tetrahydromethanopterin + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19053"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58018"/>
            <dbReference type="ChEBI" id="CHEBI:58337"/>
            <dbReference type="EC" id="3.5.4.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the MCH family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.27" evidence="3 10 11"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323033.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWF4"/>
    <dbReference type="STRING" id="348780.NP_2634A"/>
    <dbReference type="EnsemblBacteria" id="CAI49408">
        <property type="protein sequence ID" value="CAI49408"/>
        <property type="gene ID" value="NP_2634A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702899"/>
    <dbReference type="KEGG" id="nph:NP_2634A"/>
    <dbReference type="eggNOG" id="arCOG02675">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_876031_0_0_2"/>
    <dbReference type="OrthoDB" id="105468at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018759">
        <property type="term" value="F:methenyltetrahydromethanopterin cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.340.11">
        <property type="entry name" value="Methenyltetrahydromethanopterin Cyclohydrolase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1030.10">
        <property type="entry name" value="Methenyltetrahydromethanopterin Cyclohydrolase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00486">
        <property type="entry name" value="McH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003209">
        <property type="entry name" value="METHMP_CycHdrlase"/>
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        <property type="entry name" value="one_C_mch"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="MCH"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Methenyltetrahydromethanopterin cyclohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 10" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006902"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="NCBIfam" id="TIGR03120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI49408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="313" mass="32702" checksum="50F86F5DE2E5B1FF" modified="2017-05-10" version="1">MESLNRMAVELVDEAVDFADELAIEIEELDNGAMVCDFGVEATGGIEAGLLLTEIQTGGLATVQTTVGEVADAPLTHVEVSSDHPGIAFLAAQKAGWEISVDGFEGLGSGPARALVAEEEEHQRLGYRDAADFAVLAIESDRLPTEAVAEQVAERAGVPTSSVFLPTFSTASITGSVALAARAGELAAFRLSELGYDPLDMLSATASAPVAPIAGDDETAMARTNDALAYGGRVHLTVDSDFERLSEATSTASDEFGTPFADIFESHEWDFQDLPVEVFAPAQVTFDVAGGGLDVVGELNEELLAESFGIESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWK6</accession>
    <name>A0A1U7EWK6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histidine kinase</fullName>
            <ecNumber evidence="3">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">NP_2742A</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323087.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWK6"/>
    <dbReference type="STRING" id="348780.NP_2742A"/>
    <dbReference type="EnsemblBacteria" id="CAI49462">
        <property type="protein sequence ID" value="CAI49462"/>
        <property type="gene ID" value="NP_2742A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701541"/>
    <dbReference type="KEGG" id="nph:NP_2742A"/>
    <dbReference type="eggNOG" id="arCOG02352">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02365">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02388">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_58_2"/>
    <dbReference type="OrthoDB" id="230688at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.100:FF:000001">
        <property type="entry name" value="Sensory transduction histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
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        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
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        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052162">
        <property type="entry name" value="Sensor_kinase/Photoreceptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
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        <property type="entry name" value="sensory_box"/>
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    </dbReference>
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        <property type="entry name" value="PAC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43304">
        <property type="entry name" value="PHYTOCHROME-LIKE PROTEIN CPH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="PAS" evidence="15">
        <location>
            <begin position="149"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="16">
        <location>
            <begin position="222"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="15">
        <location>
            <begin position="275"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="16">
        <location>
            <begin position="347"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="16">
        <location>
            <begin position="469"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="15">
        <location>
            <begin position="522"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="16">
        <location>
            <begin position="597"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="14">
        <location>
            <begin position="653"/>
            <end position="857"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAI49462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="857" mass="95907" checksum="C0D4AAD6C3D83191" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWL0</accession>
    <name>A0A1U7EWL0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">HTH-10 family transcription regulator</fullName>
        </submittedName>
    </protein>
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        <name evidence="5" type="ordered locus">NP_2794A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
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        <lineage>
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            <taxon>Methanobacteriati</taxon>
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            <taxon>Stenosarchaea group</taxon>
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            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EnsemblBacteria" id="CAI49488">
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        <property type="gene ID" value="NP_2794A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701562"/>
    <dbReference type="KEGG" id="nph:NP_2794A"/>
    <dbReference type="eggNOG" id="arCOG02274">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092678_0_0_2"/>
    <dbReference type="OrthoDB" id="194393at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
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    </dbReference>
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        <property type="entry name" value="HTH_bacterioopsin"/>
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        <property type="entry name" value="HVO_0513_N"/>
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    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236:SF1">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04967">
        <property type="entry name" value="HTH_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24278">
        <property type="entry name" value="HVO_0513_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="C-terminal effector domain of the bipartite response regulators"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HVO-0513-like N-terminal" evidence="4">
        <location>
            <begin position="17"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="HTH bat-type" evidence="3">
        <location>
            <begin position="160"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="216" mass="23708" checksum="622974BB5A89F068" modified="2017-05-10" version="1">MRYVKVSLVPEDGEIDPTGGTVAASPDHNYEAILHTNRLNDGTAVYLLKISGGPDGLDELFEASDNVLSYDISTLRDGVQAYLHTEPTDVGQALHDLTQRHELVIDMPIEYGPNGGISVASIGGEETVKEAIDDIPENLRVELERLSDYDPELQELSAMLTDRQREILDTAAELGYYQVPREATHQDIADHLGLSTTTVGEHLRKIEARMLSEIAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWN6</accession>
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        <recommendedName>
            <fullName evidence="4 5">Large ribosomal subunit protein eL18</fullName>
        </recommendedName>
    </protein>
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        <name evidence="5 7" type="primary">rpl18e</name>
        <name evidence="7" type="ordered locus">NP_2836A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eL18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
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    <dbReference type="STRING" id="348780.NP_2836A"/>
    <dbReference type="EnsemblBacteria" id="CAI49509">
        <property type="protein sequence ID" value="CAI49509"/>
        <property type="gene ID" value="NP_2836A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703128"/>
    <dbReference type="KEGG" id="nph:NP_2836A"/>
    <dbReference type="eggNOG" id="arCOG00780">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_146465_0_0_2"/>
    <dbReference type="OrthoDB" id="11309at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:structural constituent of ribosome"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="entry name" value="50S ribosomal protein L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal_eL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000039">
        <property type="entry name" value="Ribosomal_eL18"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal_eL18/eL18-A/B/_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022947">
        <property type="entry name" value="Ribosomal_eL18_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001196">
        <property type="entry name" value="Ribosomal_uL15_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003079">
        <property type="entry name" value="PRK04005.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10934">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10934:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00475">
        <property type="entry name" value="RIBOSOMAL_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01106">
        <property type="entry name" value="RIBOSOMAL_L18E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
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    <feature type="domain" description="Large ribosomal subunit protein uL15/eL18" evidence="6">
        <location>
            <begin position="64"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="117" mass="12646" checksum="6AFD8D6A6A701B08" modified="2017-05-10" version="1">MSSKTNPRLSSLIADLKSTARNGGGEVWSDVAERLEKPRSTHAEVNLGRIERYAREDETVIVPGKVLGSGALRKEVTVAAVDFSSTAQTKIDQVGDAIELEQALEDNPDGSNVRVIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWR0</accession>
    <name>A0A1U7EWR0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ion channel pore / TrkA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">pchA2</name>
        <name evidence="8" type="ordered locus">NP_2920A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a potassium transport system.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323175.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWR0"/>
    <dbReference type="STRING" id="348780.NP_2920A"/>
    <dbReference type="EnsemblBacteria" id="CAI49551">
        <property type="protein sequence ID" value="CAI49551"/>
        <property type="gene ID" value="NP_2920A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701599"/>
    <dbReference type="KEGG" id="nph:NP_2920A"/>
    <dbReference type="eggNOG" id="arCOG01960">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035216_0_0_2"/>
    <dbReference type="OrthoDB" id="43518at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015079">
        <property type="term" value="F:potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="entry name" value="Regulator of K+ conductance, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006036">
        <property type="entry name" value="K_uptake_TrkA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050721">
        <property type="entry name" value="Trk_Ktr_HKT_K-transport"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43833:SF13">
        <property type="entry name" value="POTASSIUM CHANNEL PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43833">
        <property type="entry name" value="POTASSIUM CHANNEL PROTEIN 2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00335">
        <property type="entry name" value="KUPTAKETRKA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="TrkA C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0630">Potassium</keyword>
    <keyword evidence="2" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="66"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="6">
        <location>
            <begin position="109"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="7">
        <location>
            <begin position="247"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="7">
        <location>
            <begin position="452"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="534" mass="58028" checksum="DCCCBF743AB8FE40" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWT0</accession>
    <name>A0A1U7EWT0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">KaiC-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2904A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323168.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWT0"/>
    <dbReference type="STRING" id="348780.NP_2904A"/>
    <dbReference type="EnsemblBacteria" id="CAI49543">
        <property type="protein sequence ID" value="CAI49543"/>
        <property type="gene ID" value="NP_2904A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701591"/>
    <dbReference type="KEGG" id="nph:NP_2904A"/>
    <dbReference type="eggNOG" id="arCOG02452">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102056_0_0_2"/>
    <dbReference type="OrthoDB" id="70318at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055927">
        <property type="entry name" value="DUF7504"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24336">
        <property type="entry name" value="DUF7504"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="197" mass="21450" checksum="8391E502CE193FF0" modified="2017-05-10" version="1">MSYSTTDLGNASAFDEGVSLLMTGASTVTNQRLLDAVAPEDGERAIVITMNMGAQQVVKELERRGADRDQLGIIDCTSTEADVEGVPVRQLSSPGDLTGISLEFAKLLDQDSEDESVRARIGFASVSTALMYAELRTMFRFLHVFTARIRSGDMLGVFAMDPSMHEDQAHNTIRAVFDCEAEVTDDDITVRGTGFEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWT8</accession>
    <name>A0A1U7EWT8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NRAMP family transport protein (Probable substrate divalent metal cation)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">mntH</name>
        <name evidence="7" type="synonym">tp36</name>
        <name evidence="7" type="ordered locus">NP_2956A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323193.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWT8"/>
    <dbReference type="STRING" id="348780.NP_2956A"/>
    <dbReference type="EnsemblBacteria" id="CAI49569">
        <property type="protein sequence ID" value="CAI49569"/>
        <property type="gene ID" value="NP_2956A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703312"/>
    <dbReference type="KEGG" id="nph:NP_2956A"/>
    <dbReference type="eggNOG" id="arCOG04531">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020088_6_3_2"/>
    <dbReference type="OrthoDB" id="211791at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015086">
        <property type="term" value="F:cadmium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034755">
        <property type="term" value="P:iron ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001046">
        <property type="entry name" value="NRAMP_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037982">
        <property type="entry name" value="Nramp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706:SF33">
        <property type="entry name" value="NATURAL RESISTANCE-ASSOCIATED MACROPHAGE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706">
        <property type="entry name" value="SOLUTE CARRIER PROTEIN FAMILY 11 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01566">
        <property type="entry name" value="Nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="38"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="123"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="227"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="268"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="314"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="337"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="378"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="401" mass="41351" checksum="3659273C0743DEE7" modified="2017-05-10" version="1">MGLLARLKAIGPGALVAAAFVGPGTVTTASVIGAEYAYLLVWTIAFSILATMVLQEMSARLGLITRDGLGEALRNEFSNPLAKWATVGLVVSAIGIGTAAFQTGNIVGGAAGLATITGVSENVWGPAIGLVAAALLWSGNYKLIERVFIALVAVMGVAFILNAIIVRPDLGALGGGLVPTIPDGSAYLIVGLIGTTVVGYNLFLHASTVQERWDDKSDLAECRTDTIAMVAVGGIITTAIVVTAAAVFPPGTEIDDVGEMANQLEPVFGGYALTFFAVGLFAAGFTSAMSAPLAGAYATAGALGWERDLTSMRFRAIWMTILGVGIVGSAMDLNPVSVIVFAQVANGLLLPILAVFLIYAMNNDDLLGEYTNSRLQNILGGLVTLVVVGIGLQTLYDVLVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWU8</accession>
    <name>A0A1U7EWU8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UspA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2976A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323203.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWU8"/>
    <dbReference type="STRING" id="348780.NP_2976A"/>
    <dbReference type="EnsemblBacteria" id="CAI49579">
        <property type="protein sequence ID" value="CAI49579"/>
        <property type="gene ID" value="NP_2976A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701613"/>
    <dbReference type="KEGG" id="nph:NP_2976A"/>
    <dbReference type="eggNOG" id="arCOG03050">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_19_1_2"/>
    <dbReference type="OrthoDB" id="281037at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF6">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN UP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="130" mass="13905" checksum="5029CD5B649C10A4" modified="2017-05-10" version="1">MTHIVLVIDDDEERARTAAEQVTDLDWDTDALDVTVLHVFTDNVEGATISQFGPAREARDVLEAAGVDVTLAETSGDPADKIVTYAADEDADVICLSGRKRSPAGKAVFGSVSQSVMLNSDRPVLFCPPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWW7</accession>
    <name>A0A1U7EWW7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Aldehyde dehydrogenase</fullName>
            <ecNumber evidence="7">1.2.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">aldH2</name>
        <name evidence="7" type="ordered locus">NP_3020A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.3" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323224.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWW7"/>
    <dbReference type="STRING" id="348780.NP_3020A"/>
    <dbReference type="EnsemblBacteria" id="CAI49601">
        <property type="protein sequence ID" value="CAI49601"/>
        <property type="gene ID" value="NP_3020A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702501"/>
    <dbReference type="KEGG" id="nph:NP_3020A"/>
    <dbReference type="eggNOG" id="arCOG01252">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_1_0_2"/>
    <dbReference type="OrthoDB" id="6342at2157"/>
    <dbReference type="BRENDA" id="1.2.1.3">
        <property type="organism ID" value="3575"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000007">
        <property type="entry name" value="NAD/NADP-dependent betaine aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044638">
        <property type="entry name" value="ALDH7A1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43521">
        <property type="entry name" value="ALPHA-AMINOADIPIC SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43521:SF1">
        <property type="entry name" value="ALPHA-AMINOADIPIC SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="6">
        <location>
            <begin position="12"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="505" mass="54983" checksum="B3A8F6E117C32961" modified="2017-05-10" version="1">MSVSEQYIDGGWTDGDGTETFESKNPATGEVVGTFRRGTDTDVSRAVAAAADAFEAWRTLSRIDRAEHLWEIYHRLQERHEELGEVVTKECGKEISEGKADVAEAWHMVEWAAGDARHPKGDVVPSEISSKDSYMRRKPRGVTACITPWNFPVAIPFWHMAVALVEGNTVVWKPAEQTPRCGEAVAELFDDAGVPDGVFNMVQGFGDAGAALVDDDRVDTVLFTGSAEVGHEIAEQVATAPGKRAACEMGGKNAVVITEHADLDIAVHAAVMSAFKTTGQRCVSSERLVVHTDIYEAFKERFVEIARTVAVGDPLSEETFMGPLIEADHYENVATYNQLARDEGVNVLVDRTALDSEAVPEGHDDGHWVGPFVYEAAPDADLRCTSEEVFGPHVALLEYDGGIERAVEIHNDTDYGLAGAVISEDYRQIHYYRDHADVGLAYANLPCIGAEVQLPFGGVGKSGNGYPSAREVIEAVTDRTAWTLNNARDIEMAQGLSADIRTQKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWY4</accession>
    <name>A0A1U7EWY4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ATP:cob(I)alamin adenosyltransferase</fullName>
            <ecNumber evidence="7">2.5.1.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pduO</name>
        <name evidence="7" type="ordered locus">NP_3008A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.5.1.17" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323219.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWY4"/>
    <dbReference type="STRING" id="348780.NP_3008A"/>
    <dbReference type="EnsemblBacteria" id="CAI49595">
        <property type="protein sequence ID" value="CAI49595"/>
        <property type="gene ID" value="NP_3008A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701625"/>
    <dbReference type="KEGG" id="nph:NP_3008A"/>
    <dbReference type="eggNOG" id="arCOG00489">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083486_0_0_2"/>
    <dbReference type="OrthoDB" id="4665at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008817">
        <property type="term" value="F:corrinoid adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1200.10:FF:000003">
        <property type="entry name" value="ATP:cob(I)alamin adenosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1200.10">
        <property type="entry name" value="Cobalamin adenosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016030">
        <property type="entry name" value="CblAdoTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036451">
        <property type="entry name" value="CblAdoTrfase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029499">
        <property type="entry name" value="PduO-typ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00636">
        <property type="entry name" value="PduO_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12213">
        <property type="entry name" value="CORRINOID ADENOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12213:SF0">
        <property type="entry name" value="CORRINOID ADENOSYLTRANSFERASE MMAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01923">
        <property type="entry name" value="Cob_adeno_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89028">
        <property type="entry name" value="Cobalamin adenosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cobalamin adenosyltransferase-like" evidence="6">
        <location>
            <begin position="3"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="178" mass="19744" checksum="E49566638A3804F8" modified="2017-05-10" version="1">MKIYTGRGDEGMTDLRDMSRVSKTSARIEAYGTVDEVNSLVGMVRPSGHDDVDEKLAAVQNHLHIIQADFANPDADDPDAPHIEADHVERLESWMDDFDDELDPLESFILPGGSDTGAKLHHARSVCRRAERRAVALASDEPVNDAAVAYLNRLSDALFVWARVVNKRDGVREESPSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX46</accession>
    <name>A0A1U7EX46_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">tRNA-t(6)A37 methylthiotransferase</fullName>
            <ecNumber evidence="11">2.8.4.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">NP_3198A</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:37075"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="Rhea" id="RHEA-COMP:11092"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:74420"/>
            <dbReference type="EC" id="2.8.4.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="11">Binds 1 or 2 [4Fe-4S] cluster. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the methylthiotransferase family. CDKAL1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.5" evidence="11"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323312.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX46"/>
    <dbReference type="STRING" id="348780.NP_3198A"/>
    <dbReference type="EnsemblBacteria" id="CAI49690">
        <property type="protein sequence ID" value="CAI49690"/>
        <property type="gene ID" value="NP_3198A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701682"/>
    <dbReference type="KEGG" id="nph:NP_3198A"/>
    <dbReference type="eggNOG" id="arCOG01358">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018697_4_2_2"/>
    <dbReference type="OrthoDB" id="372134at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035598">
        <property type="term" value="F:tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12160">
        <property type="entry name" value="Methylthiotransferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="entry name" value="tm_1862 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005839">
        <property type="entry name" value="Methylthiotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020612">
        <property type="entry name" value="Methylthiotransferase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013848">
        <property type="entry name" value="Methylthiotransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038135">
        <property type="entry name" value="Methylthiotransferase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006466">
        <property type="entry name" value="MiaB-like_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01578">
        <property type="entry name" value="MiaB-like-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00089">
        <property type="entry name" value="MiaB/RimO family radical SAM methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11918">
        <property type="entry name" value="RADICAL SAM PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11918:SF45">
        <property type="entry name" value="THREONYLCARBAMOYLADENOSINE TRNA METHYLTHIOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00919">
        <property type="entry name" value="UPF0004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51449">
        <property type="entry name" value="MTTASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01278">
        <property type="entry name" value="MTTASE_RADICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8 11" id="KW-0408">Iron</keyword>
    <keyword evidence="9 11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="MTTase N-terminal" evidence="13">
        <location>
            <begin position="2"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="14">
        <location>
            <begin position="117"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="12">
        <location>
            <begin position="351"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAI49690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="414" mass="45934" checksum="C7DED4B5A2AB9475" modified="2017-05-10" version="1">MATYHIETYGCTSNRGESRQIERKLRDAGHYRVDTPEKADVAILNTCTVVEKTERNMLRRAEELEAETADLIVTGCMALAQGDEFGDIDAQVLHWEDVPTAVTNGECPTTTPDAEPVLDGVVGILPIARGCMSNCSYCITKFATGRVDSPPVAENVEKARALVHAGAKELRITGQDTGVYGWDTGERKLPELLDRICDIDGEFRVRLGMANPGGVHGIHEELADVFAENEKLYNFIHAPVQSGSDDVLEDMRRQHRVEKFKDIVDAFDERLDHWTLSTDFIVGFPTEDEADHELSMELLAEVRPEKINVTRFSKRPGTDAADMKGLGGTIKKERSKAMTDLKMDICREVYGSMVGETREVLVVEQGTGDSVKCRDEAYRQVIVTDATEHDIDPGDMLDVTITDSETVYCFGEPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX65</accession>
    <name>A0A1U7EX65_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable glycosyltransferase, type 1</fullName>
            <ecNumber evidence="2">2.4.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">gth2</name>
        <name evidence="2" type="ordered locus">NP_3216A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.4.-.-" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323321.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX65"/>
    <dbReference type="STRING" id="348780.NP_3216A"/>
    <dbReference type="EnsemblBacteria" id="CAI49699">
        <property type="protein sequence ID" value="CAI49699"/>
        <property type="gene ID" value="NP_3216A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701686"/>
    <dbReference type="KEGG" id="nph:NP_3216A"/>
    <dbReference type="eggNOG" id="arCOG01407">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055069_0_0_2"/>
    <dbReference type="OrthoDB" id="17979at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03801">
        <property type="entry name" value="GT4_PimA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050194">
        <property type="entry name" value="Glycosyltransferase_grp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45947">
        <property type="entry name" value="SULFOQUINOVOSYL TRANSFERASE SQD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45947:SF3">
        <property type="entry name" value="SULFOQUINOVOSYL TRANSFERASE SQD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13692">
        <property type="entry name" value="Glyco_trans_1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase subfamily 4-like N-terminal" evidence="1">
        <location>
            <begin position="32"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="358" mass="39762" checksum="6628265A5875548A" modified="2017-05-10" version="1">MRVLNYLELEARLQRSGIGTATRQQRRALSPTDVEVVKTPWNGANPAEAAFRRAVGGRFFERVDLAHCNLVGPGSAAVARRAIRTGTPLVLHAHITREDFAGSFRGSRAVGPVLERYLRWFYSQADLVVCPSEHTKRVLSSYPVDAPVRVVTNGVDIESLAGHEALRDRYRSRFDLDGTVVFAVGNVFERKGLTAFCRLAERTDYEFVWFGPYDTGPHASPTVRRWVKDPPENVTFTGWVDDIRGAYAAGDIYLFPTKAENQGIAVLEAMACGNAVILRDIPVFEEFYTHGEDCLKCDTRSEFEAAIDRLASDPDLRARLGDTAATTAREHGLDRVAEELRDAYSDAQRLAGAEHTTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXC5</accession>
    <name>A0A1U7EXC5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Propionyl-CoA carboxylase carboxyltransferase component</fullName>
            <ecNumber evidence="3">6.4.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">pccB3</name>
        <name evidence="3" type="ordered locus">NP_3464A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.4.1.3" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323443.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXC5"/>
    <dbReference type="STRING" id="348780.NP_3464A"/>
    <dbReference type="EnsemblBacteria" id="CAI49823">
        <property type="protein sequence ID" value="CAI49823"/>
        <property type="gene ID" value="NP_3464A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702471"/>
    <dbReference type="KEGG" id="nph:NP_3464A"/>
    <dbReference type="eggNOG" id="arCOG02705">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018822_6_1_2"/>
    <dbReference type="OrthoDB" id="30579at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905202">
        <property type="term" value="C:methylcrotonoyl-CoA carboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004485">
        <property type="term" value="F:methylcrotonoyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004658">
        <property type="term" value="F:propionyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006552">
        <property type="term" value="P:L-leucine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045190">
        <property type="entry name" value="MCCB/AccD1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22855">
        <property type="entry name" value="ACETYL, PROPIONYL, PYRUVATE, AND GLUTACONYL CARBOXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22855:SF13">
        <property type="entry name" value="METHYLCROTONOYL-COA CARBOXYLASE BETA CHAIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="1">
        <location>
            <begin position="52"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="2">
        <location>
            <begin position="315"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="565" mass="60898" checksum="A33A8F38A0C71172" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXF0</accession>
    <name>A0A1U7EXF0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">KaiC domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3516A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323469.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXF0"/>
    <dbReference type="STRING" id="348780.NP_3516A"/>
    <dbReference type="EnsemblBacteria" id="CAI49849">
        <property type="protein sequence ID" value="CAI49849"/>
        <property type="gene ID" value="NP_3516A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701792"/>
    <dbReference type="KEGG" id="nph:NP_3516A"/>
    <dbReference type="eggNOG" id="arCOG01175">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069300_1_0_2"/>
    <dbReference type="OrthoDB" id="49711at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055549">
        <property type="entry name" value="DUF7125"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637:SF2">
        <property type="entry name" value="PROTEIN GVPD 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637">
        <property type="entry name" value="UPF0273 PROTEIN TM_0370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23442">
        <property type="entry name" value="DUF7125"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="208" mass="22385" checksum="E8F03F42E8EF00A8" modified="2017-05-10" version="1">MPERLRTGVDALDRQLEGGLPPGSVVALAAAPASQAELLLYELTATRETLYLSIDRTRDAVATSIDNAPTETGSPTVRDVAGEAPLDNAAKLVSALPEGANLIVDPVDPLERNEAARYRKFLNELKNHVTNTQGLAVLHCLDGRDVPRLRDTTAHMADVVFELETTIAGDRVENRLAVPKFRGGRALSDVLKLDLAAEVDIDTSRDIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXF1</accession>
    <name>A0A1U7EXF1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histidine kinase</fullName>
            <ecNumber evidence="3">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">NP_3536A</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323479.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXF1"/>
    <dbReference type="STRING" id="348780.NP_3536A"/>
    <dbReference type="EnsemblBacteria" id="CAI49859">
        <property type="protein sequence ID" value="CAI49859"/>
        <property type="gene ID" value="NP_3536A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701799"/>
    <dbReference type="KEGG" id="nph:NP_3536A"/>
    <dbReference type="eggNOG" id="arCOG02327">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_58_2"/>
    <dbReference type="OrthoDB" id="230688at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000156">
        <property type="term" value="F:phosphorelay response regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007234">
        <property type="term" value="P:osmosensory signaling via phosphorelay pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00075">
        <property type="entry name" value="HATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050351">
        <property type="entry name" value="BphY/WalK/GraS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031621">
        <property type="entry name" value="HisKA_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013656">
        <property type="entry name" value="PAS_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42878:SF7">
        <property type="entry name" value="SENSOR HISTIDINE KINASE GLRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42878">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16927">
        <property type="entry name" value="HisKA_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08448">
        <property type="entry name" value="PAS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="72"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="15">
        <location>
            <begin position="344"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="14">
        <location>
            <begin position="482"/>
            <end position="684"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAI49859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="685" mass="74378" checksum="25D5B9491AA05800" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXW9</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3886A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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            <taxon>Archaea</taxon>
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            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Gabara</strain>
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    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50034.1"/>
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    <dbReference type="InterPro" id="IPR036890">
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    <dbReference type="NCBIfam" id="NF047352">
        <property type="entry name" value="P_loop_sacsin"/>
        <property type="match status" value="1"/>
    </dbReference>
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            <end position="909"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1733"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="880"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1753"/>
            <end position="1762"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="1999" mass="223569" checksum="E697FAA2D5A652C3" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXZ8</accession>
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        <submittedName>
            <fullName evidence="2">Glyoxalase domain protein</fullName>
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    </protein>
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        <name evidence="2" type="ordered locus">NP_3948A</name>
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    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
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    <dbReference type="EMBL" id="CR936257">
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    <dbReference type="GeneID" id="3702756"/>
    <dbReference type="KEGG" id="nph:NP_3948A"/>
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    <dbReference type="HOGENOM" id="CLU_1352088_0_0_2"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Extradiol_RC_dioxygenase"/>
    </dbReference>
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        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
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        <property type="entry name" value="VOC_core"/>
    </dbReference>
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        <property type="entry name" value="RING-CLEAVING DIOXYGENASE MHQA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36110">
        <property type="entry name" value="RING-CLEAVING DIOXYGENASE MHQE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="22"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="203" mass="22026" checksum="0A86D038329456A5" modified="2017-05-10" version="1">MEHTPEHDVTAAPPESPVHTIGTDHMTVMGGDAASTIAFYRDLLGMPLVLEQPNLDAPELTHLFFDTGDGRILTFFVGEGRSPRPYRGAGNVHHLAFSIAPDSFEETKTALDDAGYGFNEFDRGAFHSLYTQDPSGLVVELVVEKYDFPPERRGEVLALAHEKRVEAGAGYVDDEHMQAALSELGLDSEPRFEGSAPTGAGVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY06</accession>
    <name>A0A1U7EY06_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Pyrroline-5-carboxylate reductase</fullName>
            <shortName evidence="4">P5C reductase</shortName>
            <shortName evidence="4">P5CR</shortName>
            <ecNumber evidence="4 5">1.5.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">PCA reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4 9" type="primary">proC</name>
        <name evidence="9" type="ordered locus">NP_3974A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the reduction of 1-pyrroline-5-carboxylate (PCA) to L-proline.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-proline + NAD(+) = (S)-1-pyrroline-5-carboxylate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14105"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-proline + NADP(+) = (S)-1-pyrroline-5-carboxylate + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the pyrroline-5-carboxylate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.2" evidence="4 5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323694.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY06"/>
    <dbReference type="STRING" id="348780.NP_3974A"/>
    <dbReference type="EnsemblBacteria" id="CAI50078">
        <property type="protein sequence ID" value="CAI50078"/>
        <property type="gene ID" value="NP_3974A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703056"/>
    <dbReference type="KEGG" id="nph:NP_3974A"/>
    <dbReference type="eggNOG" id="arCOG00455">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042344_0_2_2"/>
    <dbReference type="OrthoDB" id="25257at2157"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00361"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004735">
        <property type="term" value="F:pyrroline-5-carboxylate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3730.10:FF:000001">
        <property type="entry name" value="Pyrroline-5-carboxylate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.10">
        <property type="entry name" value="ProC C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01925">
        <property type="entry name" value="P5C_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029036">
        <property type="entry name" value="P5CR_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000304">
        <property type="entry name" value="Pyrroline-COOH_reductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00112">
        <property type="entry name" value="proC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645">
        <property type="entry name" value="PYRROLINE-5-CARBOXYLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645:SF0">
        <property type="entry name" value="PYRROLINE-5-CARBOXYLATE REDUCTASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14748">
        <property type="entry name" value="P5CR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000193">
        <property type="entry name" value="Pyrrol-5-carb_rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4 6" id="KW-0521">NADP</keyword>
    <keyword evidence="3 4 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase catalytic N-terminal" evidence="7">
        <location>
            <begin position="4"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase dimerisation" evidence="8">
        <location>
            <begin position="146"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="7"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="63"/>
            <end position="66"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="NCBIfam" id="TIGR00112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000193-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI50078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="257" mass="26970" checksum="2A57C1B887C87F23" modified="2017-05-10" version="1">MTSVSVIGCGNMGGALLRGLAESGNYTLTACDLDPEAREAVEEYAETTDDPAVAADADIVVVAVKPHIARDVVDDIELSSDQTLVTIAAGVSTDALADRTDATVVRVMPNLAAETRNMAAAVTESTVTDDVRSMLDDVGEFVDIDEELMDIATAVNGSGPAFVFYLIDAMRQAGVESGLDDEDARVLAAQTFKGAAETVLRSDEDIETLIDAVCSPNGTTIEGMDVLWDSDADEAVVDAVAAAERRSKELADESDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY45</accession>
    <name>A0A1U7EY45_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DUF371 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4060A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323737.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY45"/>
    <dbReference type="STRING" id="348780.NP_4060A"/>
    <dbReference type="EnsemblBacteria" id="CAI50121">
        <property type="protein sequence ID" value="CAI50121"/>
        <property type="gene ID" value="NP_4060A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701954"/>
    <dbReference type="KEGG" id="nph:NP_4060A"/>
    <dbReference type="eggNOG" id="arCOG04171">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135994_0_0_2"/>
    <dbReference type="OrthoDB" id="9265at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.630">
        <property type="entry name" value="mth639 domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007171">
        <property type="entry name" value="DUF371"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023131">
        <property type="entry name" value="Mth639-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40696:SF1">
        <property type="entry name" value="DUF371 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40696">
        <property type="entry name" value="DUF371 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04027">
        <property type="entry name" value="DUF371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="138" mass="14705" checksum="909C4FE9D98749AF" modified="2017-05-10" version="1">MEKVIRASGHENVSAEHSSTFELTSDDWLTPAGDCILGVEADTVPADFDDAFVEACQSHEATITATLRVGDETQVIEGHGHPELTFESDRSLVGRTSDYVDDRTVMVGASAAAADIDRAIADRLADGADIECRLAVDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYC6</accession>
    <name>A0A1U7EYC6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4298A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323856.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYC6"/>
    <dbReference type="STRING" id="348780.NP_4298A"/>
    <dbReference type="EnsemblBacteria" id="CAI50240">
        <property type="protein sequence ID" value="CAI50240"/>
        <property type="gene ID" value="NP_4298A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702024"/>
    <dbReference type="KEGG" id="nph:NP_4298A"/>
    <dbReference type="eggNOG" id="arCOG11980">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1159085_0_0_2"/>
    <dbReference type="OrthoDB" id="186030at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="227" mass="25158" checksum="DD57B002C1A61D7C" modified="2017-05-10" version="1">MSESTAESIVRRYVKQPWRDLIRIYYTNTPVWRWLKSGALVVLGFCLWTGGNVLLSVQPDWTFLHYVMSYGLIVVVWGPLTHFVIVPLTIRLRRTAEHPAVRWLAKRSGKIQFSLFVLVVIVVGAAAPGVMFLDFSVSIEGGGTAVGGELTCETADGLVSCHVADPQGIDHVVVTSGGETLATVEEEPFAFELDTDDIAETRTGKEFRVDYRNADGETVYRQVEHVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYC7</accession>
    <name>A0A1U7EYC7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">acetate--CoA ligase</fullName>
            <ecNumber evidence="2">6.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">acs1</name>
        <name evidence="10" type="ordered locus">NP_4240A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.1" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323827.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYC7"/>
    <dbReference type="STRING" id="348780.NP_4240A"/>
    <dbReference type="EnsemblBacteria" id="CAI50211">
        <property type="protein sequence ID" value="CAI50211"/>
        <property type="gene ID" value="NP_4240A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702484"/>
    <dbReference type="KEGG" id="nph:NP_4240A"/>
    <dbReference type="eggNOG" id="arCOG01529">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_3_6_2"/>
    <dbReference type="OrthoDB" id="371752at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006085">
        <property type="term" value="P:acetyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095:SF14">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="9">
        <location>
            <begin position="37"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="7">
        <location>
            <begin position="98"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="8">
        <location>
            <begin position="543"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="634"/>
            <end position="655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI50211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="655" mass="72823" checksum="D73E0F4E1A0E5D57" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYG2</accession>
    <name>A0A1U7EYG2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Sec-independent protein translocase protein TatA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">tatA1</name>
        <name evidence="11" type="ordered locus">NP_4348A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323881.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYG2"/>
    <dbReference type="STRING" id="348780.NP_4348A"/>
    <dbReference type="EnsemblBacteria" id="CAI50265">
        <property type="protein sequence ID" value="CAI50265"/>
        <property type="gene ID" value="NP_4348A"/>
    </dbReference>
    <dbReference type="GeneID" id="95969897"/>
    <dbReference type="KEGG" id="nph:NP_4348A"/>
    <dbReference type="eggNOG" id="arCOG02694">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1830641_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043953">
        <property type="term" value="P:protein transport by the Tat complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.3310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003369">
        <property type="entry name" value="TatA/B/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006312">
        <property type="entry name" value="TatA/E"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01411">
        <property type="entry name" value="tatAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42982">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42982:SF1">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02416">
        <property type="entry name" value="TatA_B_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="54"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="76"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="108"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI50265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="145" mass="15085" checksum="B439A8BB24C9FF79" modified="2017-05-10" version="1">MIDLLPLQFGIPGGMELLVVVLIAVLLFGADKVPKLARSTGEAMGEFQKGREEIEEELEEVKGGSSSSSPQTAVDRAVEQTKRAESGSAGGSTAADTAADEMSKSDDTESTDAEADDSDTDDTAESAEDDADEPVDIADAESKTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYG5</accession>
    <name>A0A1U7EYG5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Cyclase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4388A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323901.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYG5"/>
    <dbReference type="STRING" id="348780.NP_4388A"/>
    <dbReference type="EnsemblBacteria" id="CAI50285">
        <property type="protein sequence ID" value="CAI50285"/>
        <property type="gene ID" value="NP_4388A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702045"/>
    <dbReference type="KEGG" id="nph:NP_4388A"/>
    <dbReference type="eggNOG" id="arCOG02462">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030671_3_3_2"/>
    <dbReference type="OrthoDB" id="9014at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004061">
        <property type="term" value="F:arylformamidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019441">
        <property type="term" value="P:L-tryptophan catabolic process to kynurenine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.50">
        <property type="entry name" value="Putative cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007325">
        <property type="entry name" value="KFase/CYL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037175">
        <property type="entry name" value="KFase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31118">
        <property type="entry name" value="CYCLASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31118:SF12">
        <property type="entry name" value="CYCLASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04199">
        <property type="entry name" value="Cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102198">
        <property type="entry name" value="Putative cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="265" mass="28703" checksum="069A8C956255353E" modified="2017-05-10" version="1">MFADATIVDLSIGIEDGAASEPDPATIDRMDHREGAEWLAENLQAMGVDVSADDFPDGTALAWEEIEAITHTGTHMDAPYHYGPESGGDPARRIDDVPLDWCLGEAVVLDVRHLDGGDEITVPHLEAALSDLGYELSGDEIVLIQTGADELWGSAEYLTDFPGMGRDATLWLVEQGVRVIGTDAYGFDKPFLEMGRRFEANGESEELWPAHLAGREREYCQIEKMANLDALPRKTAVPLVAAPVVVEDGSAGWVRPLAVFPEGTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYR5</accession>
    <name>A0A1U7EYR5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4600A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324006.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYR5"/>
    <dbReference type="STRING" id="348780.NP_4600A"/>
    <dbReference type="EnsemblBacteria" id="CAI50391">
        <property type="protein sequence ID" value="CAI50391"/>
        <property type="gene ID" value="NP_4600A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702110"/>
    <dbReference type="KEGG" id="nph:NP_4600A"/>
    <dbReference type="eggNOG" id="arCOG06209">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2021435_0_0_2"/>
    <dbReference type="OrthoDB" id="209683at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="129" mass="14409" checksum="C71C9021528390F5" modified="2017-05-10" version="1">MSVADDDTPTFAIPERPVRRYPRSGGVEYDGETVFSLQPATARSDDALAAAIERVLDEGPYRYGDWFDLPMPVYIVHDDETGDTFRVAVRDGAIELHVLPGTEPPGLAAFYRRVRARSDHAWRVTTRTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYR8</accession>
    <name>A0A1U7EYR8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">WD40/YVTN domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_4608A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324010.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYR8"/>
    <dbReference type="STRING" id="348780.NP_4608A"/>
    <dbReference type="EnsemblBacteria" id="CAI50395">
        <property type="protein sequence ID" value="CAI50395"/>
        <property type="gene ID" value="NP_4608A"/>
    </dbReference>
    <dbReference type="GeneID" id="43500102"/>
    <dbReference type="KEGG" id="nph:NP_4608A"/>
    <dbReference type="eggNOG" id="arCOG02560">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_686276_0_0_2"/>
    <dbReference type="OrthoDB" id="167723at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051200">
        <property type="entry name" value="Host-pathogen_enzymatic-act"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011045">
        <property type="entry name" value="N2O_reductase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47197:SF3">
        <property type="entry name" value="DIHYDRO-HEME D1 DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47197">
        <property type="entry name" value="PROTEIN NIRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50974">
        <property type="entry name" value="Nitrous oxide reductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="38"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="243"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="49"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="249"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="474" mass="50069" checksum="565B8DB5EC155D67" modified="2017-05-10" version="1">MRDDPRPEQRLSPSQHVSRRRLIQGIGAGATVMLAGCAEENGNGATEQTPDDDDETGTEPDDGEEELTAAAYAFTDTAVVGLDTETEATAEVESVPNASWEDVIATPEYLFAVENGSSEVYVLDPGTDEITDRIPVGTNPVHGFYAESTDEVWVHSDDEAQFYVVDAAAPDSVEARVAVGDVGAGHGKVAATGDGRAVVTNMEAGGLFVVNLEAKETTEHIEFDADYHSHSHSGHQTLELLHDAGDDHDHDDDDHDHDDDDHDHSDGTHYVAYAPESGLAFVEELGGGHHQHSMSGSKETQRLTARGVSPLHGDDDGGDARTVVVDVDNAEVEGTIDAGGPTYGADAHELVSVIDGDDVHFVDSTDSHGHADETVTLSDASPAAVRFGADGAYTANPHSGDISILDIESYEELERLSVPGDHLDDMPSVIADETFATVAADTAVLVDLESQDPVQFDVSDIQTVGLLSAGAVTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYU5</accession>
    <name>A0A1U7EYU5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DUF11/DUF58 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_4646A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50414.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324028.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYU5"/>
    <dbReference type="STRING" id="348780.NP_4646A"/>
    <dbReference type="EnsemblBacteria" id="CAI50414">
        <property type="protein sequence ID" value="CAI50414"/>
        <property type="gene ID" value="NP_4646A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702132"/>
    <dbReference type="KEGG" id="nph:NP_4646A"/>
    <dbReference type="eggNOG" id="arCOG02742">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026973_1_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047589">
        <property type="entry name" value="DUF11_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002881">
        <property type="entry name" value="DUF58"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001434">
        <property type="entry name" value="OmcB-like_DUF11"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01451">
        <property type="entry name" value="B_ant_repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33608">
        <property type="entry name" value="BLL2464 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33608:SF6">
        <property type="entry name" value="BLL2464 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01345">
        <property type="entry name" value="DUF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01882">
        <property type="entry name" value="DUF58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF11" evidence="1">
        <location>
            <begin position="57"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="DUF58" evidence="2">
        <location>
            <begin position="199"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50414.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="456" mass="47789" checksum="83232E132A566965" modified="2017-05-10" version="1">MGTRHINRLNSGVTASATLVAAGVTFGSAALLLAAAVPITYLGYAAVSSVPDPEAKISVTRELTPRNPLPGRRVEVTLTVRNESSRTLADVRIVDQIPDNLELADGKASSAVTLQAGEATEITYTLRPRRGTYVFDDAWVRVRSLSAASIGTASIPVTGDTELECTVPLDGLPVYRETVATTGSVASDRGGNGTEFYATREYRHGDSLSRIDWRRYARTGELGTVLYREQETTNIIVLVDGREKAGVAPAPGQPGGLTLAAYAGLITSSAAANAGHNVGVVGLGVDSELPGVYRGPPAYVPPGNGADIGGRIARVCDAIAARGSGTELSTTDGDEHTAGDACTTKADGGTQNTQAAGVSIARLEALLPATAQLVVCTPAIDDEGIDLIAKLRQRGYPTAVVSPDVNDHDSIGARLADVRRQARLERLRRLDVAVTDWDPKTPLASAFNRGIDEVIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ35</accession>
    <name>A0A1U7EZ35_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7975 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4908A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50545.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939726.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ35"/>
    <dbReference type="STRING" id="348780.NP_4908A"/>
    <dbReference type="EnsemblBacteria" id="CAI50545">
        <property type="protein sequence ID" value="CAI50545"/>
        <property type="gene ID" value="NP_4908A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702198"/>
    <dbReference type="KEGG" id="nph:NP_4908A"/>
    <dbReference type="eggNOG" id="arCOG06416">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2115550_0_0_2"/>
    <dbReference type="OrthoDB" id="193911at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058281">
        <property type="entry name" value="DUF7975"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25930">
        <property type="entry name" value="DUF7975"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7975" evidence="1">
        <location>
            <begin position="3"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50545.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="114" mass="12830" checksum="4D8AAD69105C97EE" modified="2017-05-10" version="1">MGATDAEGRRRLLVEHILAVRRDGGTVTFVAGDTRVGYEDRRLRLDAAADERERLATLLSEYHVFKIKQPETRTADDGVVYLSAVTDAKHAADFVEALFRTVYGADEDYELRVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ38</accession>
    <name>A0A1U7EZ38_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DUF3368 domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4840A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ38"/>
    <dbReference type="STRING" id="348780.NP_4840A"/>
    <dbReference type="EnsemblBacteria" id="CAI50511">
        <property type="protein sequence ID" value="CAI50511"/>
        <property type="gene ID" value="NP_4840A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702191"/>
    <dbReference type="KEGG" id="nph:NP_4840A"/>
    <dbReference type="eggNOG" id="arCOG00717">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115769_0_1_2"/>
    <dbReference type="OrthoDB" id="323844at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021799">
        <property type="entry name" value="PIN-like_prokaryotic"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39550">
        <property type="entry name" value="SLL0658 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39550:SF1">
        <property type="entry name" value="SLL0658 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11848">
        <property type="entry name" value="DUF3368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="169" mass="18100" checksum="16305568E374AF32" modified="2017-05-10" version="1">MWVFDATPLIYLAKAEQLEMVAALEKRCVLPGSVYEEVVEAGIEQGYPDACRVEHAVTDGTLAVVEGEETPLLGRLREADGLTTADASVLACAATHDGVAVMDESYGRNIAAAEGIETRGTAYLVLLAVKRGTIDAEAGREAIDSMLDAGWYCSPDVYGKVVRKLESFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ73</accession>
    <name>A0A1U7EZ73_NATPD</name>
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        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="5" type="ordered locus">NP_5030A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324216.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ73"/>
    <dbReference type="STRING" id="348780.NP_5030A"/>
    <dbReference type="EnsemblBacteria" id="CAI50606">
        <property type="protein sequence ID" value="CAI50606"/>
        <property type="gene ID" value="NP_5030A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702234"/>
    <dbReference type="KEGG" id="nph:NP_5030A"/>
    <dbReference type="eggNOG" id="arCOG03052">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049558_1_0_2"/>
    <dbReference type="OrthoDB" id="201701at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055520">
        <property type="entry name" value="DUF7094"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055522">
        <property type="entry name" value="DUF7096"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056397">
        <property type="entry name" value="Fn3_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23375">
        <property type="entry name" value="DUF7094"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23379">
        <property type="entry name" value="DUF7096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23374">
        <property type="entry name" value="Fn3_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7096" evidence="4">
        <location>
            <begin position="1"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7094" evidence="3">
        <location>
            <begin position="226"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin-III type-like" evidence="2">
        <location>
            <begin position="343"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI50606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="420" mass="44502" checksum="BEC514673BB38DC4" modified="2017-05-10" version="1">MNGARAVLCALLICVATLGGVVIAADSPDGASAAEPTVSATDAPLPQTDGESDRLLSIVSEGDTAEYLDPPPGTIDRSGQETVIVDVSSAVGTNSDTIRSEFRQESLVAAFEEATTDAERNDIAADAATRLDERADALIAAERTAIAEYNDGAIGEREFLSRIAAIDRAADRTATTADWLRDDAETHLSDEAQQQVATDAAQLLRLQGPVRERTAAALGGDEPVRVNVDTREEAVVLATVDDGTYVREAVDPTAQTVHIEDRYDDDTVLALSRFQELYPWVDDARSGTSISLFNSDHPPTRIYQFEIPHTQGELMAYLDGGSADIIHETHRLDPESMPATTYEASDNGLALQVNATRGGGPLGIEVVDADTGDDVPATVRVNESEIGTADDGQLWTVSPRGAVTVTASHGGDSVSLETEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ74</accession>
    <name>A0A1U7EZ74_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Probable anaerobic dehydrogenase membrane anchor subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">NP_4958A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NrfD family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324182.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ74"/>
    <dbReference type="STRING" id="348780.NP_4958A"/>
    <dbReference type="EnsemblBacteria" id="CAI50570">
        <property type="protein sequence ID" value="CAI50570"/>
        <property type="gene ID" value="NP_4958A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702213"/>
    <dbReference type="KEGG" id="nph:NP_4958A"/>
    <dbReference type="eggNOG" id="arCOG02027">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045348_1_0_2"/>
    <dbReference type="OrthoDB" id="205836at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1630.10">
        <property type="entry name" value="Formate dehydrogenase/DMSO reductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052049">
        <property type="entry name" value="Electron_transfer_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005614">
        <property type="entry name" value="NrfD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34856">
        <property type="entry name" value="PROTEIN NRFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34856:SF2">
        <property type="entry name" value="PROTEIN NRFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03916">
        <property type="entry name" value="NrfD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="18"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="92"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="184"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="220"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="255"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="297"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="333"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI50570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="367" mass="37624" checksum="FF32B88D06FF1CEE" modified="2017-05-10" version="1">MPLEIIFNEPYTQWDWKIAQYVALIGLAGGAYLTAYVADVLSKRRESVEHGQLAKYGYLTGLLVIAISPPIMLSHLATPFRAMALPFTMSNLGSWMAIGSYLIAGFGLGALLMFGWTAFGKERPNRPAVADGGTDIASDGGTAETATGGRTVGGGVRGVANRVGLLDLLDRLADITRPSEKIRLGLGAFFGVLAAGVLLYSAMALGSGATNRVPLWDKTFLIPVQILSGLGAGLAAAVGLTALATRSPGRTLQSYALAASGLLVGSLLAVLATVTMLPGQAPAAAEAVNNMLTTYAGLFIGVAIVGGMVLPAIFLGGSALAQRSGALPDDVTAASYVFAGALAVAGKIALALVYILAAEFTPLPLPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZB2</accession>
    <name>A0A1U7EZB2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">mcm2</name>
        <name evidence="15" type="ordered locus">NP_5106A</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324254.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZB2"/>
    <dbReference type="STRING" id="348780.NP_5106A"/>
    <dbReference type="EnsemblBacteria" id="CAI50644">
        <property type="protein sequence ID" value="CAI50644"/>
        <property type="gene ID" value="NP_5106A"/>
    </dbReference>
    <dbReference type="GeneID" id="32155026"/>
    <dbReference type="KEGG" id="nph:NP_5106A"/>
    <dbReference type="eggNOG" id="arCOG00439">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG03146">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000995_7_2_2"/>
    <dbReference type="OrthoDB" id="6747at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
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        <property type="entry name" value="Cell division control protein 21"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DNA helicase"/>
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        <property type="entry name" value="DNA replication licensing factor (Mcm)"/>
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        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
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        <property type="entry name" value="Nucleic acid-binding proteins"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
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        <property type="entry name" value="NA-bd_OB-fold"/>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
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        <property type="entry name" value="intein_Nterm"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM4"/>
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    </dbReference>
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        <property type="entry name" value="HTH_3"/>
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        <property type="entry name" value="INTEIN"/>
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        <property type="entry name" value="MCMFAMILY"/>
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        <property type="entry name" value="HintC"/>
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        <property type="entry name" value="Hedgehog/intein (Hint) domain"/>
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        <property type="entry name" value="Nucleic acid-binding proteins"/>
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        <property type="entry name" value="HTH_CROC1"/>
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        <property type="entry name" value="INTEIN_N_TER"/>
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        <property type="entry name" value="MCM_2"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0651">Protein splicing</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="275"/>
            <end position="331"/>
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        <location>
            <begin position="495"/>
            <end position="545"/>
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    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="13">
        <location>
            <begin position="662"/>
            <end position="822"/>
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    </feature>
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        <location>
            <begin position="1009"/>
            <end position="1037"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAI50644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="1037" mass="115345" checksum="B3508B1532D995A4" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZE1</accession>
    <name>A0A1U7EZE1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Acetate--CoA ligase</fullName>
            <ecNumber evidence="2 6">6.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">acs4</name>
        <name evidence="11" type="ordered locus">NP_5132A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
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        <property type="protein sequence ID" value="CAI50657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324267.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A1U7EZE1"/>
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    <dbReference type="EnsemblBacteria" id="CAI50657">
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        <property type="gene ID" value="NP_5132A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702487"/>
    <dbReference type="KEGG" id="nph:NP_5132A"/>
    <dbReference type="eggNOG" id="arCOG04201">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_3_6_2"/>
    <dbReference type="OrthoDB" id="371752at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043955">
        <property type="term" value="F:3-hydroxypropionyl-CoA synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019427">
        <property type="term" value="P:acetyl-CoA biosynthetic process from acetate"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043427">
        <property type="term" value="P:carbon fixation by 3-hydroxypropionate cycle"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Acetyl-coenzyme A synthetase"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
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    </dbReference>
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        <property type="entry name" value="Ac_CoA_lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02188">
        <property type="entry name" value="Ac_CoA_lig_AcsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001208">
        <property type="entry name" value="PRK00174.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095:SF14">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="10">
        <location>
            <begin position="35"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="8">
        <location>
            <begin position="89"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="9">
        <location>
            <begin position="542"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="632"/>
            <end position="657"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="NCBIfam" id="TIGR02188"/>
        </source>
    </evidence>
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        <source>
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    <dbReference type="EnsemblBacteria" id="CAI50939">
        <property type="protein sequence ID" value="CAI50939"/>
        <property type="gene ID" value="NP_7046A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703483"/>
    <dbReference type="KEGG" id="nph:NP_3322A"/>
    <dbReference type="KEGG" id="nph:NP_7046A"/>
    <dbReference type="HOGENOM" id="CLU_293328_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="278"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="422"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="488"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="693"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="866"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="995"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="278"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="336"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="422"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="437"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="488"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="702"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="750"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="866"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="995"/>
            <end position="1006"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="1036" mass="112343" checksum="D999EC55742F6397" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILW5</accession>
    <name>Q3ILW5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ISH9-type transposase ISNph4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_6250A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324502.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILW5"/>
    <dbReference type="EnsemblBacteria" id="CAI50905">
        <property type="protein sequence ID" value="CAI50905"/>
        <property type="gene ID" value="NP_6250A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694655"/>
    <dbReference type="KEGG" id="nph:NP_6250A"/>
    <dbReference type="HOGENOM" id="CLU_088837_0_0_2"/>
    <dbReference type="OrthoDB" id="110773at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053520">
        <property type="entry name" value="Transposase_Tn903"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033579">
        <property type="entry name" value="transpos_IS5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS4-like" evidence="1">
        <location>
            <begin position="107"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="274" mass="31698" checksum="A5742C168110BBAA" modified="2005-11-08" version="1">MEVDLLDFVEQCRHLVKQALGKHAGEPASGGFARWKHVVLHCFRLEEDHSYRETPNRLEYMAEIRDVLDLDRDDLPDYSTIYKSFDRLKMWVWRALLRVSAQQHPQSGHAALDSTFFDRRSASSYYRQRSGSNVQTLKVTTLTDRESLAVLDVHISARWKHDTKTGPQVVRRNADDLLSVAADKAFHNWITKYEFYALGVEPLILQRGSRPLTVGYNALIRTKGYAQRWMAETSYSTTKRSLGDAVRALGWYRQFREIVLMFATSNIEPLCEPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILX9</accession>
    <name>Q3ILX9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">HTH-10 family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_6220A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4 5">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324488.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILX9"/>
    <dbReference type="EnsemblBacteria" id="CAI50890">
        <property type="protein sequence ID" value="CAI50890"/>
        <property type="gene ID" value="NP_6220A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694641"/>
    <dbReference type="KEGG" id="nph:NP_6220A"/>
    <dbReference type="HOGENOM" id="CLU_1275276_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007050">
        <property type="entry name" value="HTH_bacterioopsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236:SF1">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04967">
        <property type="entry name" value="HTH_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH bat-type" evidence="3">
        <location>
            <begin position="147"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="203" mass="23049" checksum="E50690F0AB026328" modified="2005-11-08" version="1">MDGMFTATVHFTQHRECILRELTADVERPVPIEIEELQNGVVTFVLQAGSHADAFQTELEAADHVKHVKRLNEENLLVTKPSCGAYSAIYQNHGTLQRSNIVNGRQREYNILVFQRSDLKDIIADLREFGTVTLGRLEEFKTTDSPLTARQQEVVSEALTRGYYEWPREITSEELAAELDISRATLHEHLRKAEQTLLSSEVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILZ6</accession>
    <name>Q3ILZ6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Aldehyde ferredoxin oxidoreductase</fullName>
            <ecNumber evidence="10">1.2.7.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">aor3</name>
        <name evidence="10" type="ordered locus">NP_6186A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10 11">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>tungstopterin</name>
            <dbReference type="ChEBI" id="CHEBI:30402"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AOR/FOR family.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.5" evidence="10"/>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324471.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILZ6"/>
    <dbReference type="EnsemblBacteria" id="CAI50873">
        <property type="protein sequence ID" value="CAI50873"/>
        <property type="gene ID" value="NP_6186A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694680"/>
    <dbReference type="KEGG" id="nph:NP_6186A"/>
    <dbReference type="HOGENOM" id="CLU_020364_1_0_2"/>
    <dbReference type="OrthoDB" id="30771at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033726">
        <property type="term" value="F:aldehyde ferredoxin oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.569.10">
        <property type="entry name" value="Aldehyde Ferredoxin Oxidoreductase Protein, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.599.10">
        <property type="entry name" value="Aldehyde Ferredoxin Oxidoreductase Protein, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.9.10">
        <property type="entry name" value="Aldehyde ferredoxin oxidoreductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013984">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013985">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013983">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036503">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001203">
        <property type="entry name" value="OxRdtase_Ald_Fedxn_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036021">
        <property type="entry name" value="Tungsten_al_ferr_oxy-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051919">
        <property type="entry name" value="W-dependent_AOR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30038">
        <property type="entry name" value="ALDEHYDE FERREDOXIN OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30038:SF7">
        <property type="entry name" value="TUNGSTEN-CONTAINING GLYCERALDEHYDE-3-PHOSPHATE:FERREDOXIN OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01314">
        <property type="entry name" value="AFOR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02730">
        <property type="entry name" value="AFOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00790">
        <property type="entry name" value="AFOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48310">
        <property type="entry name" value="Aldehyde ferredoxin oxidoreductase, C-terminal domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56228">
        <property type="entry name" value="Aldehyde ferredoxin oxidoreductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde ferredoxin oxidoreductase N-terminal" evidence="9">
        <location>
            <begin position="5"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI50873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="554" mass="60405" checksum="7D5991F8488BD3FB" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-28" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMM9</accession>
    <name>DPCKG_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GTP-dependent dephospho-CoA kinase</fullName>
            <ecNumber evidence="1">2.7.1.237</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Dephospho-coenzyme A kinase</fullName>
            <shortName evidence="1">DPCK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">NP_5076A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the GTP-dependent phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A (CoA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3'-dephospho-CoA + GTP = GDP + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61156"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="2.7.1.237"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP-dependent DPCK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.237" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324239.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMM9"/>
    <dbReference type="SMR" id="Q3IMM9"/>
    <dbReference type="STRING" id="348780.NP_5076A"/>
    <dbReference type="EnsemblBacteria" id="CAI50629">
        <property type="protein sequence ID" value="CAI50629"/>
        <property type="gene ID" value="NP_5076A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702247"/>
    <dbReference type="KEGG" id="nph:NP_5076A"/>
    <dbReference type="eggNOG" id="arCOG04076">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120795_0_0_2"/>
    <dbReference type="OrthoDB" id="15447at2157"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00590">
        <property type="entry name" value="Dephospho_CoA_kinase_GTP_dep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007164">
        <property type="entry name" value="GTP-dep_dephospho-CoA_kin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40732:SF1">
        <property type="entry name" value="GTP-DEPENDENT DEPHOSPHO-COA KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40732">
        <property type="entry name" value="UPF0218 PROTEIN TK1697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04019">
        <property type="entry name" value="DUF359"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006533">
        <property type="entry name" value="UCP006533"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000380063" description="GTP-dependent dephospho-CoA kinase">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00590"/>
        </source>
    </evidence>
    <sequence length="178" mass="19210" checksum="E952E47DE04B1BCD" modified="2005-11-08" version="1">MSKILAKLPTEMRGELKDPLGEIYTDTDELLADAGDPLVAVGDIVTYHLLEAGHRPAAAIVDGKTERERVERKVLSAIEEFDERIDVANPQSTITDDLLEALSTALDRPDPTVVVVDGEEDLASLPAVVAAPVGASVVYGQPGEGMVLVHVTDETQAECRDIIERMQSDYSQIESILS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMV8</accession>
    <name>KCY_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cytidylate kinase</fullName>
            <shortName evidence="1">CK</shortName>
            <ecNumber evidence="1">2.7.4.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cytidine monophosphate kinase</fullName>
            <shortName evidence="1">CMP kinase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cmk</name>
        <name type="ordered locus">NP_4914A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>CMP + ATP = CDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:11600"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>dCMP + ATP = dCDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:25094"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57566"/>
            <dbReference type="ChEBI" id="CHEBI:58593"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.25"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytidylate kinase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.25" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324160.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMV8"/>
    <dbReference type="SMR" id="Q3IMV8"/>
    <dbReference type="STRING" id="348780.NP_4914A"/>
    <dbReference type="EnsemblBacteria" id="CAI50548">
        <property type="protein sequence ID" value="CAI50548"/>
        <property type="gene ID" value="NP_4914A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702613"/>
    <dbReference type="KEGG" id="nph:NP_4914A"/>
    <dbReference type="eggNOG" id="arCOG01037">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079959_1_0_2"/>
    <dbReference type="OrthoDB" id="31096at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036430">
        <property type="term" value="F:CMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036431">
        <property type="term" value="F:dCMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006220">
        <property type="term" value="P:pyrimidine nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02020">
        <property type="entry name" value="CMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00239">
        <property type="entry name" value="Cytidyl_kinase_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011892">
        <property type="entry name" value="Cyt_kin_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011994">
        <property type="entry name" value="Cytidylate_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02173">
        <property type="entry name" value="cyt_kin_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13189">
        <property type="entry name" value="Cytidylate_kin2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000005682" description="Cytidylate kinase">
        <location>
            <begin position="1"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00239"/>
        </source>
    </evidence>
    <sequence length="192" mass="21385" checksum="5605D39E62C00A87" modified="2005-11-08" version="1">MLITISGPAGSGKSTVAAGLAESLGYEHVSGGDIFRDLADDRGLTPLELNKRAEEDDQIDRDLDRKQRDIAESRDDIVLESRLAGWMAGEHADFRIWLDAPLSVRAERIADREDKSVELAHNETKERGKSEALRYREYYNIDIEDRSIYDLALNTARLSPDGVRAVVESAVNAYAPDDDEGQTPVEGVTYEF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-09-05" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMX0</accession>
    <name>RL19E_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein eL19</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">50S ribosomal protein L19e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl19e</name>
        <name type="ordered locus">NP_4890A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324148.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMX0"/>
    <dbReference type="SMR" id="Q3IMX0"/>
    <dbReference type="STRING" id="348780.NP_4890A"/>
    <dbReference type="EnsemblBacteria" id="CAI50536">
        <property type="protein sequence ID" value="CAI50536"/>
        <property type="gene ID" value="NP_4890A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703129"/>
    <dbReference type="KEGG" id="nph:NP_4890A"/>
    <dbReference type="eggNOG" id="arCOG04089">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083919_1_1_2"/>
    <dbReference type="OrthoDB" id="11624at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01418">
        <property type="entry name" value="Ribosomal_L19e_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1650.10:FF:000001">
        <property type="entry name" value="Ribosomal protein L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.560">
        <property type="entry name" value="Single Heli x bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01475">
        <property type="entry name" value="Ribosomal_eL19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035970">
        <property type="entry name" value="60S_ribosomal_eL19_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039547">
        <property type="entry name" value="Ribosomal_eL19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033936">
        <property type="entry name" value="Ribosomal_eL19_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000196">
        <property type="entry name" value="Ribosomal_eL19_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015972">
        <property type="entry name" value="Ribosomal_eL19_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015973">
        <property type="entry name" value="Ribosomal_eL19_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015974">
        <property type="entry name" value="Ribosomal_eL19_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057259">
        <property type="entry name" value="Ribosomal_L19e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057260">
        <property type="entry name" value="Ribosomal_L19e_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006343">
        <property type="entry name" value="PRK08570.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01280">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25476">
        <property type="entry name" value="Ribosomal_L19e_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01416">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48140">
        <property type="entry name" value="Ribosomal protein L19 (L19e)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000419045" description="Large ribosomal subunit protein eL19">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="46"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="59"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="89"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="149" mass="17127" checksum="3A9727EB1840FC0E" modified="2005-11-08" version="1">MTDLKAQKRLAADILDVGENRVRFDPDAQAEIADAITREDIRELIEDGTIEAKTAKGNSRGRARKRQQKRAYGHKKGHGSRKGRSGGRQNEKEDWQSRIRAQRRELRELRDAGDISREQYRELYDMASGGEFDSVADLNRYIDDKHGEQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-16" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q3INV7</accession>
    <name>SURE_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">5'-nucleotidase SurE</fullName>
            <ecNumber evidence="1">3.1.3.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Nucleoside 5'-monophosphate phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">surE</name>
        <name type="ordered locus">NP_4208A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Nucleotidase that shows phosphatase activity on nucleoside 5'-monophosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-phosphate + H2O = a ribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:12484"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18254"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
            <dbReference type="EC" id="3.1.3.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="1">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SurE nucleotidase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.5" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323811.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3INV7"/>
    <dbReference type="SMR" id="Q3INV7"/>
    <dbReference type="STRING" id="348780.NP_4208A"/>
    <dbReference type="EnsemblBacteria" id="CAI50195">
        <property type="protein sequence ID" value="CAI50195"/>
        <property type="gene ID" value="NP_4208A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703224"/>
    <dbReference type="KEGG" id="nph:NP_4208A"/>
    <dbReference type="eggNOG" id="arCOG02303">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045192_1_3_2"/>
    <dbReference type="OrthoDB" id="26873at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008253">
        <property type="term" value="F:5'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1210.10">
        <property type="entry name" value="Survival protein SurE-like phosphatase/nucleotidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00060">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030048">
        <property type="entry name" value="SurE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002828">
        <property type="entry name" value="SurE-like_Pase/nucleotidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036523">
        <property type="entry name" value="SurE-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00087">
        <property type="entry name" value="surE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30457">
        <property type="entry name" value="5'-NUCLEOTIDASE SURE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30457:SF0">
        <property type="entry name" value="PHOSPHATASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G01070)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01975">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64167">
        <property type="entry name" value="SurE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000235680" description="5'-nucleotidase SurE">
        <location>
            <begin position="1"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="234"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="257" mass="26966" checksum="3532C616A2073451" modified="2005-11-08" version="1">MDVLLTNDDGIDAVGIRALYDALAEVADVTAVAPADDQSAVGRQLSRTVELHDHELGYAVEGTPADCVIAGLGALDLDPDIVVAGCNEGANLGEYVLGRSGTVSAAVEAAFFGVPAIAASVYFPAGDVTIEEFDPDKTDFAEASRAVRYLVDNAIGAGVFDAADYLNVNAPLPPETGHAPMEITEPSHVYEMDGERDGETVRIQDHIWERMAEGTIPDPPGTDRRAVVEGRVSVSPLTAPHPTTGHEGLAGLAEKYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q3INX3</accession>
    <name>Q3INX3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Probable archaetidylserine decarboxylase</fullName>
            <ecNumber evidence="12">4.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">psd</name>
        <name evidence="12" type="ordered locus">NP_4176A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.1.1.-" evidence="12"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323795.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3INX3"/>
    <dbReference type="STRING" id="348780.NP_4176A"/>
    <dbReference type="EnsemblBacteria" id="CAI50179">
        <property type="protein sequence ID" value="CAI50179"/>
        <property type="gene ID" value="NP_4176A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703060"/>
    <dbReference type="KEGG" id="nph:NP_4176A"/>
    <dbReference type="eggNOG" id="arCOG04470">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072492_1_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004609">
        <property type="term" value="F:phosphatidylserine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003817">
        <property type="entry name" value="PS_Dcarbxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033175">
        <property type="entry name" value="PSD-A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038088">
        <property type="entry name" value="anchor_synt_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003683">
        <property type="entry name" value="PRK05305.2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35809">
        <property type="entry name" value="ARCHAETIDYLSERINE DECARBOXYLASE PROENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35809:SF1">
        <property type="entry name" value="ARCHAETIDYLSERINE DECARBOXYLASE PROENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02666">
        <property type="entry name" value="PS_Dcarbxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="10" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI50179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="200" mass="21899" checksum="2563161B5D69759F" modified="2005-11-08" version="1">MFPSIPSVAPGTWRYARFPLVAAVVFAVLWPPLALACLAATVGVYWFHRDPERSPPPTGVVSPADGRVSVVRTEGQRVRVAVFMNVTDVHINRAPAAGTVTSVEHTPGGHLPAFSKKSDRNERVTVEFEEYELTLIAGTVARRVYPYVAAGDELDRGERLGHISFSSRADVLLPPEYDRSDIRVEEGQHVRAGETVIAAR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-26" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IPU7</accession>
    <name>PYRI_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aspartate carbamoyltransferase regulatory chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrI</name>
        <name type="ordered locus">NP_3520A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in allosteric regulation of aspartate carbamoyltransferase.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Contains catalytic and regulatory chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PyrI family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323471.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IPU7"/>
    <dbReference type="SMR" id="Q3IPU7"/>
    <dbReference type="STRING" id="348780.NP_3520A"/>
    <dbReference type="EnsemblBacteria" id="CAI49851">
        <property type="protein sequence ID" value="CAI49851"/>
        <property type="gene ID" value="NP_3520A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703088"/>
    <dbReference type="KEGG" id="nph:NP_3520A"/>
    <dbReference type="eggNOG" id="arCOG04229">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128576_0_0_2"/>
    <dbReference type="OrthoDB" id="7000at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009347">
        <property type="term" value="C:aspartate carbamoyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.20">
        <property type="entry name" value="Aspartate carbamoyltransferase regulatory subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.140">
        <property type="entry name" value="Aspartate carbamoyltransferase regulatory subunit, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00002">
        <property type="entry name" value="Asp_carb_tr_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020545">
        <property type="entry name" value="Asp_carbamoyltransf_reg_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002801">
        <property type="entry name" value="Asp_carbamoylTrfase_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020542">
        <property type="entry name" value="Asp_carbamoyltrfase_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036792">
        <property type="entry name" value="Asp_carbatrfase_reg_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036793">
        <property type="entry name" value="Asp_carbatrfase_reg_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00240">
        <property type="entry name" value="ATCase_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35805">
        <property type="entry name" value="ASPARTATE CARBAMOYLTRANSFERASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35805:SF1">
        <property type="entry name" value="ASPARTATE CARBAMOYLTRANSFERASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01948">
        <property type="entry name" value="PyrI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02748">
        <property type="entry name" value="PyrI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57825">
        <property type="entry name" value="Aspartate carbamoyltransferase, Regulatory-chain, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54893">
        <property type="entry name" value="Aspartate carbamoyltransferase, Regulatory-chain, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000321508" description="Aspartate carbamoyltransferase regulatory chain">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00002"/>
        </source>
    </evidence>
    <sequence length="153" mass="16439" checksum="0E3A48E3BEFB26F9" modified="2005-11-08" version="1">MTDTELRVSKIQKGTVIDHIAGGQALNVLAILGIDGTSGDEISVGMNVPSDRLGRKDIVKVEGRELSQNEVDVLSLIAPAATINIVRDFEVIEKHRVTRPETVEGVLSCPNANCITTENEPVDSRFEVLEAGVRCSYCGTIIRESLAAHISVA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IQ38</accession>
    <name>TRPD_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Anthranilate phosphoribosyltransferase</fullName>
            <ecNumber evidence="1">2.4.2.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trpD</name>
        <name type="ordered locus">NP_3338A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of the phosphoribosyl group of 5-phosphorylribose-1-pyrophosphate (PRPP) to anthranilate to yield N-(5'-phosphoribosyl)-anthranilate (PRA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N-(5-phospho-beta-D-ribosyl)anthranilate + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + anthranilate</text>
            <dbReference type="Rhea" id="RHEA:11768"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:18277"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium ions per monomer.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the anthranilate phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.18" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323380.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IQ38"/>
    <dbReference type="SMR" id="Q3IQ38"/>
    <dbReference type="STRING" id="348780.NP_3338A"/>
    <dbReference type="EnsemblBacteria" id="CAI49760">
        <property type="protein sequence ID" value="CAI49760"/>
        <property type="gene ID" value="NP_3338A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703411"/>
    <dbReference type="KEGG" id="nph:NP_3338A"/>
    <dbReference type="eggNOG" id="arCOG02012">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034315_2_1_2"/>
    <dbReference type="OrthoDB" id="8214at2157"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00041"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004048">
        <property type="term" value="F:anthranilate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1030.10:FF:000002">
        <property type="entry name" value="Anthranilate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1030.10">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.970.10">
        <property type="entry name" value="Transferase, Pyrimidine Nucleoside Phosphorylase, Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00211">
        <property type="entry name" value="TrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005940">
        <property type="entry name" value="Anthranilate_Pribosyl_Tfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000312">
        <property type="entry name" value="Glycosyl_Trfase_fam3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017459">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036320">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035902">
        <property type="entry name" value="Nuc_phospho_transferase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01245">
        <property type="entry name" value="trpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43285">
        <property type="entry name" value="ANTHRANILATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43285:SF2">
        <property type="entry name" value="ANTHRANILATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02885">
        <property type="entry name" value="Glycos_trans_3N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00591">
        <property type="entry name" value="Glycos_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52418">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47648">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="chain" id="PRO_0000227203" description="Anthranilate phosphoribosyltransferase">
        <location>
            <begin position="1"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="81"/>
            <end position="82"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="88"/>
            <end position="91"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="106"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00211"/>
        </source>
    </evidence>
    <sequence length="333" mass="34162" checksum="40B1BB01E362C5F0" modified="2005-11-08" version="1">MKDYIRRVSDGEDLTQAEAREAASLVFEEATEAQIGALLSALRAKGETEPEIAGFAEGMRAAARRIDPDRSPLVDTCGTGGDGHDTINVSTTSSFVVAGAGVPVAKHGNYSVSSSSGSADVLEEIGVTIDAEPPAVETCIEETGMGFMLAPVFHPAMKAVIGPRKELGMRTIFNVLGPLTNPADADAQVVGVYDEALVPTLADALSRMSVDRALVVHGAGLDEIGVHGESTVAEVDGESVEQYTVTPSDLGVDSHDLSAVAGGSPTENAADMRGILEGDVEGAKRDIILANAGAAIYIAGEADSLAAGVDAARESIDTGAAAAQLASLREFEP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-10-02" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IQT9</accession>
    <name>RS13_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein uS13</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">30S ribosomal protein S13</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps13</name>
        <name type="ordered locus">NP_2828A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. In the 70S ribosome it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits; these bridges are implicated in subunit movement.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Forms a loose heterodimer with protein S19. Forms two bridges to the 50S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323130.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IQT9"/>
    <dbReference type="SMR" id="Q3IQT9"/>
    <dbReference type="STRING" id="348780.NP_2828A"/>
    <dbReference type="EnsemblBacteria" id="CAI49505">
        <property type="protein sequence ID" value="CAI49505"/>
        <property type="gene ID" value="NP_2828A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703168"/>
    <dbReference type="KEGG" id="nph:NP_2828A"/>
    <dbReference type="eggNOG" id="arCOG01722">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103849_0_0_2"/>
    <dbReference type="OrthoDB" id="372127at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.910.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.910.10">
        <property type="entry name" value="30s ribosomal protein s13, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01315">
        <property type="entry name" value="Ribosomal_uS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027437">
        <property type="entry name" value="Rbsml_uS13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001892">
        <property type="entry name" value="Ribosomal_uS13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_uS13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019977">
        <property type="entry name" value="Ribosomal_uS13_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018269">
        <property type="entry name" value="Ribosomal_uS13_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003140">
        <property type="entry name" value="PRK04053.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03629">
        <property type="entry name" value="uS13_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S13/40S RIBOSOMAL PROTEIN S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871:SF3">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00416">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002134">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="S13-like H2TH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00646">
        <property type="entry name" value="RIBOSOMAL_S13_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50159">
        <property type="entry name" value="RIBOSOMAL_S13_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000306761" description="Small ribosomal subunit protein uS13">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="128"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="128"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="170" mass="19280" checksum="5D9E6159C53CE370" modified="2005-11-08" version="1">MSTEEPQADEEDEDLQYFVRIGQTDLDGTKSVERALTDMNGIGRRVARIIAEKAEVDRRDVLGALDEEKIDDVVAAVEEYGDEVPEWLTNHQKDFFTGETTHEIGNDLQMSRRQDINRMKKIDAYRGVRHKRGQKVRGQRTKSTGRTEGTIGVNVEAIKEEQAEDGGDEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IRT2</accession>
    <name>MOBA_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable molybdenum cofactor guanylyltransferase</fullName>
            <shortName evidence="1">MoCo guanylyltransferase</shortName>
            <ecNumber evidence="1">2.7.7.77</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">GTP:molybdopterin guanylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Mo-MPT guanylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Molybdopterin guanylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Molybdopterin-guanine dinucleotide synthase</fullName>
            <shortName evidence="1">MGD synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mobA</name>
        <name type="ordered locus">NP_2138A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transfers a GMP moiety from GTP to Mo-molybdopterin (Mo-MPT) cofactor (Moco or molybdenum cofactor) to form Mo-molybdopterin guanine dinucleotide (Mo-MGD) cofactor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Mo-molybdopterin + GTP + H(+) = Mo-molybdopterin guanine dinucleotide + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:34243"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
            <dbReference type="ChEBI" id="CHEBI:71310"/>
            <dbReference type="EC" id="2.7.7.77"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">The N-terminal domain determines nucleotide recognition and specific binding, while the C-terminal domain determines the specific binding to the target protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MobA family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.77" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322788.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IRT2"/>
    <dbReference type="SMR" id="Q3IRT2"/>
    <dbReference type="STRING" id="348780.NP_2138A"/>
    <dbReference type="EnsemblBacteria" id="CAI49160">
        <property type="protein sequence ID" value="CAI49160"/>
        <property type="gene ID" value="NP_2138A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702930"/>
    <dbReference type="KEGG" id="nph:NP_2138A"/>
    <dbReference type="eggNOG" id="arCOG01872">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055597_2_1_2"/>
    <dbReference type="OrthoDB" id="28434at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061603">
        <property type="term" value="F:molybdenum cofactor guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02503">
        <property type="entry name" value="MobA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00316">
        <property type="entry name" value="MobA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013482">
        <property type="entry name" value="Molybde_CF_guanTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19136">
        <property type="entry name" value="MOLYBDENUM COFACTOR GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19136:SF81">
        <property type="entry name" value="MOLYBDENUM COFACTOR GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000019127" description="Probable molybdenum cofactor guanylyltransferase">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="9"/>
            <end position="11"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00316"/>
        </source>
    </evidence>
    <sequence length="202" mass="22038" checksum="05417C8E3B4E4382" modified="2005-11-08" version="1">MGERTGVIVAGGQSTRMGDVDKTVVDVAGVPLVRRVANRLLAVVDHLVVNCRRDQQERLAAALEGLSPTFAVDEVPDRGPTAGIMTGLSTVETPYAAVVAADMPYLDPELLEYLFERADSHDAAVPRPEEWFEPLHAVYRPEPMAEACAEALEAADARIITPLSSLDHIVIERDAIVEHGSLRSFESVDTPEDLERARERLS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-02" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IRZ5</accession>
    <name>URE1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Urease subunit alpha</fullName>
            <ecNumber evidence="1">3.5.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Urea amidohydrolase subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ureC</name>
        <name type="ordered locus">NP_2010A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>urea + 2 H2O + H(+) = hydrogencarbonate + 2 NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:20557"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16199"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ni cation</name>
            <dbReference type="ChEBI" id="CHEBI:25516"/>
        </cofactor>
        <text evidence="1">Binds 2 nickel ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (urease route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="1">Carboxylation allows a single lysine to coordinate two nickel ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. Urease alpha subunit family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.5" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322725.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IRZ5"/>
    <dbReference type="SMR" id="Q3IRZ5"/>
    <dbReference type="STRING" id="348780.NP_2010A"/>
    <dbReference type="MEROPS" id="M38.982"/>
    <dbReference type="EnsemblBacteria" id="CAI49096">
        <property type="protein sequence ID" value="CAI49096"/>
        <property type="gene ID" value="NP_2010A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703448"/>
    <dbReference type="KEGG" id="nph:NP_2010A"/>
    <dbReference type="eggNOG" id="arCOG00698">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000980_0_0_2"/>
    <dbReference type="OrthoDB" id="42542at2157"/>
    <dbReference type="UniPathway" id="UPA00258">
        <property type="reaction ID" value="UER00370"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016812">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009039">
        <property type="term" value="F:urease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043419">
        <property type="term" value="P:urea catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00375">
        <property type="entry name" value="Urease_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01953">
        <property type="entry name" value="Urease_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002195">
        <property type="entry name" value="Dihydroorotase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011612">
        <property type="entry name" value="Urease_alpha_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050112">
        <property type="entry name" value="Urease_alpha_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017950">
        <property type="entry name" value="Urease_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005848">
        <property type="entry name" value="Urease_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017951">
        <property type="entry name" value="Urease_asu_c"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009686">
        <property type="entry name" value="PRK13207.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01792">
        <property type="entry name" value="urease_alph"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43440">
        <property type="entry name" value="UREASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43440:SF1">
        <property type="entry name" value="UREASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00449">
        <property type="entry name" value="Urease_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01752">
        <property type="entry name" value="UREASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00145">
        <property type="entry name" value="UREASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51368">
        <property type="entry name" value="UREASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0533">Nickel</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000234194" description="Urease subunit alpha">
        <location>
            <begin position="1"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="domain" description="Urease" evidence="1">
        <location>
            <begin position="135"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="322"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="via carbamate group" evidence="1">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="via carbamate group" evidence="1">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="1">
        <location>
            <position position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01953"/>
        </source>
    </evidence>
    <sequence length="570" mass="60891" checksum="2ECC4AA00BD3BC6E" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IS04</accession>
    <name>RS17E_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein eS17</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">30S ribosomal protein S17e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps17e</name>
        <name type="ordered locus">NP_1992A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS17 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322716.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IS04"/>
    <dbReference type="SMR" id="Q3IS04"/>
    <dbReference type="STRING" id="348780.NP_1992A"/>
    <dbReference type="EnsemblBacteria" id="CAI49087">
        <property type="protein sequence ID" value="CAI49087"/>
        <property type="gene ID" value="NP_1992A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701326"/>
    <dbReference type="KEGG" id="nph:NP_1992A"/>
    <dbReference type="eggNOG" id="arCOG01885">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_176720_1_0_2"/>
    <dbReference type="OrthoDB" id="52479at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.20">
        <property type="entry name" value="Ribosomal protein S17e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00511">
        <property type="entry name" value="Ribosomal_eS17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001210">
        <property type="entry name" value="Ribosomal_eS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018273">
        <property type="entry name" value="Ribosomal_eS17_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036401">
        <property type="entry name" value="Ribosomal_eS17_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002242">
        <property type="entry name" value="PRK01151.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10732">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10732:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00833">
        <property type="entry name" value="Ribosomal_S17e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116820">
        <property type="entry name" value="Rps17e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00712">
        <property type="entry name" value="RIBOSOMAL_S17E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000258604" description="Small ribosomal subunit protein eS17">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="64" mass="7352" checksum="50B6EB6DBE8CB924" modified="2005-11-08" version="1">MAIKPAYVKKTGTLLMDRYPKAFGDDFEHNKELVDELTNIESKSVRNRIAGYVTRKQRSPQQPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETA3</accession>
    <name>A0A1U7ETA3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0034A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321747.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETA3"/>
    <dbReference type="EnsemblBacteria" id="CAI48108">
        <property type="protein sequence ID" value="CAI48108"/>
        <property type="gene ID" value="NP_0034A"/>
    </dbReference>
    <dbReference type="GeneID" id="43500083"/>
    <dbReference type="KEGG" id="nph:NP_0034A"/>
    <dbReference type="HOGENOM" id="CLU_2191097_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="52"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="81"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="108" mass="11122" checksum="905E86BFA4F30B08" modified="2017-05-10" version="1">MSDAGERRPDGDTEARPGGDAEERPEATDSEAAEEPADETGRPDDIVTIRTALVGLGFLWLTAVAAAVTLLSSVYGVLSSGWYLVAAVAAAGVAVTAGRRTVRTFKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETA5</accession>
    <name>A0A1U7ETA5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">peptide-methionine (R)-S-oxide reductase</fullName>
            <ecNumber evidence="3">1.8.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">msrB</name>
        <name evidence="9" type="ordered locus">NP_0048A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-methionyl-[protein] + [thioredoxin]-disulfide + H2O = L-methionyl-(R)-S-oxide-[protein] + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:24164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12314"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:45764"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MsrB Met sulfoxide reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.12" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321754.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETA5"/>
    <dbReference type="STRING" id="348780.NP_0048A"/>
    <dbReference type="EnsemblBacteria" id="CAI48115">
        <property type="protein sequence ID" value="CAI48115"/>
        <property type="gene ID" value="NP_0048A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702946"/>
    <dbReference type="KEGG" id="nph:NP_0048A"/>
    <dbReference type="eggNOG" id="arCOG02815">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031040_8_5_2"/>
    <dbReference type="OrthoDB" id="5961at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033743">
        <property type="term" value="F:peptide-methionine (R)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030091">
        <property type="term" value="P:protein repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.20:FF:000001">
        <property type="entry name" value="Peptide methionine sulfoxide reductase MsrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.20">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028427">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002579">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00357">
        <property type="entry name" value="peptide-methionine (R)-S-oxide reductase MsrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10173">
        <property type="entry name" value="METHIONINE SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10173:SF52">
        <property type="entry name" value="METHIONINE-R-SULFOXIDE REDUCTASE B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01641">
        <property type="entry name" value="SelR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51790">
        <property type="entry name" value="MSRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MsrB" evidence="8">
        <location>
            <begin position="15"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI48115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="137" mass="15410" checksum="3897A4E74213A8ED" modified="2017-05-10" version="1">MSDTDGERNDIPDSDDEWRKRLSEEEYRILREAGTEPKFSGDYLDKGDDGVYRCAGCGATLFDSETKFDSNSGWPSFYAAEDDAVEFREDRSHGMVRTEVVCARCEGHLGHVFDDGPEPTGKRFCMNSVALDFDDGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETD0</accession>
    <name>A0A1U7ETD0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transport protein (Probable substrate zinc/cadmium/cobalt)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">tp37</name>
        <name evidence="8" type="ordered locus">NP_0120A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321790.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETD0"/>
    <dbReference type="STRING" id="348780.NP_0120A"/>
    <dbReference type="EnsemblBacteria" id="CAI48151">
        <property type="protein sequence ID" value="CAI48151"/>
        <property type="gene ID" value="NP_0120A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703313"/>
    <dbReference type="KEGG" id="nph:NP_0120A"/>
    <dbReference type="eggNOG" id="arCOG01480">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021126_0_1_2"/>
    <dbReference type="OrthoDB" id="290964at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006829">
        <property type="term" value="P:zinc ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1350">
        <property type="entry name" value="Cation efflux protein, cytoplasmic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040177">
        <property type="entry name" value="SLC30A9"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13414">
        <property type="entry name" value="HUEL-CATION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13414:SF9">
        <property type="entry name" value="PROTON-COUPLED ZINC ANTIPORTER SLC30A9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160240">
        <property type="entry name" value="Cation efflux protein cytoplasmic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="7">
        <location>
            <begin position="8"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI48151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="308" mass="33053" checksum="BBC69643B14D0FA4" modified="2017-05-10" version="1">MVSTRLVVVVSLVASAAIAVAKFIAWLATGNASMLSQVYYSLSDVGNQLLFLLGFRLSDAGASRKHPFGRGKEQYVFAFVVTMLLFGVTGYAAVREGYETIGTPARDVDVTINYIVLGVALLFESAAFYKSYQAVREEADTEGFRSLFDTFRRSKDAPLLTAATENLVAVVGVLVALAGVYLTDVTGNTTYDAIGSAVIGLLLMSVALGLAWESRGLLVGEGVTRRERRRLLDAIAAADGVNAVVDLRTMHLGPESVLVACEIDFDNDLDTRGIEAAIDAVEVAVRDVVPEANRIYVEAESRPVTADR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETD2</accession>
    <name>A0A1U7ETD2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-directed RNA polymerase subunit Rpo5</fullName>
            <ecNumber evidence="4">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">DNA-directed RNA polymerase subunit H</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4 6" type="primary">rpoH</name>
        <name evidence="4" type="synonym">rpo5</name>
        <name evidence="6" type="ordered locus">NP_0108A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the archaeal Rpo5/eukaryotic RPB5 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321784.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETD2"/>
    <dbReference type="STRING" id="348780.NP_0108A"/>
    <dbReference type="EnsemblBacteria" id="CAI48145">
        <property type="protein sequence ID" value="CAI48145"/>
        <property type="gene ID" value="NP_0108A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703158"/>
    <dbReference type="KEGG" id="nph:NP_0108A"/>
    <dbReference type="eggNOG" id="arCOG04258">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058320_4_0_2"/>
    <dbReference type="OrthoDB" id="30537at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.20">
        <property type="entry name" value="RPB5-like RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00025">
        <property type="entry name" value="RNApol_Rpo5_RPB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014381">
        <property type="entry name" value="Arch_Rpo5/euc_Rpb5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000783">
        <property type="entry name" value="RNA_pol_subH/Rpb5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020608">
        <property type="entry name" value="RNA_pol_subH/Rpb5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035913">
        <property type="entry name" value="RPB5-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007129">
        <property type="entry name" value="PRK09570.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10535">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10535:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01191">
        <property type="entry name" value="RNA_pol_Rpb5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55287">
        <property type="entry name" value="RPB5-like RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01110">
        <property type="entry name" value="RNA_POL_H_23KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 4 6" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4 6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RNA polymerase subunit H/Rpb5 C-terminal" evidence="5">
        <location>
            <begin position="2"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI48145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="75" mass="8601" checksum="36711A4412C58E20" modified="2017-05-10" version="1">MVDVSQHNMVPEHTVLDREEVEDVLAEYDIKRTDLPKIQRRDAALPDDAEPGNVVKIVRDSRTTDEAVVYRLVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETD5</accession>
    <name>A0A1U7ETD5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA-directed RNA polymerase subunit Rpo1C</fullName>
            <ecNumber evidence="10">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">DNA-directed RNA polymerase subunit A''</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10 13" type="primary">rpoA2</name>
        <name evidence="10" type="synonym">rpo1C</name>
        <name evidence="13" type="ordered locus">NP_0116A</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Forms part of the jaw domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="10"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321788.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETD5"/>
    <dbReference type="STRING" id="348780.NP_0116A"/>
    <dbReference type="EnsemblBacteria" id="CAI48149">
        <property type="protein sequence ID" value="CAI48149"/>
        <property type="gene ID" value="NP_0116A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703151"/>
    <dbReference type="KEGG" id="nph:NP_0116A"/>
    <dbReference type="eggNOG" id="arCOG03146">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG04256">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_351851_0_0_2"/>
    <dbReference type="OrthoDB" id="372142at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016539">
        <property type="term" value="P:intein-mediated protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00081">
        <property type="entry name" value="Hint"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06528">
        <property type="entry name" value="RNAP_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.16.10">
        <property type="entry name" value="Hedgehog/Intein (Hint) domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="entry name" value="Homing endonucleases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00411">
        <property type="entry name" value="RNApol_arch_Rpo1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003586">
        <property type="entry name" value="Hint_dom_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003587">
        <property type="entry name" value="Hint_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
        <property type="entry name" value="Hint_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006142">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030934">
        <property type="entry name" value="Intein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004042">
        <property type="entry name" value="Intein_endonuc_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006141">
        <property type="entry name" value="Intein_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004860">
        <property type="entry name" value="LAGLIDADG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012757">
        <property type="entry name" value="RPO1C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01443">
        <property type="entry name" value="intein_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01445">
        <property type="entry name" value="intein_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011491">
        <property type="entry name" value="PRK14898.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02389">
        <property type="entry name" value="RNA_pol_rpoA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376:SF32">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14528">
        <property type="entry name" value="LAGLIDADG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00379">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00305">
        <property type="entry name" value="HintC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00306">
        <property type="entry name" value="HintN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51294">
        <property type="entry name" value="Hedgehog/intein (Hint) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="Homing endonucleases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50818">
        <property type="entry name" value="INTEIN_C_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50819">
        <property type="entry name" value="INTEIN_ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50817">
        <property type="entry name" value="INTEIN_N_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 10 13" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4 10 13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="6" id="KW-0651">Protein splicing</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 10 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DOD-type homing endonuclease" evidence="12">
        <location>
            <begin position="281"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="498"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAI48149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="894" mass="97875" checksum="A7012F65829D7952" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETE1</accession>
    <name>A0A1U7ETE1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">NP_0144A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
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        </reaction>
    </comment>
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    <dbReference type="EMBL" id="CR936257">
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    </dbReference>
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    <dbReference type="AlphaFoldDB" id="A0A1U7ETE1"/>
    <dbReference type="STRING" id="348780.NP_0144A"/>
    <dbReference type="EnsemblBacteria" id="CAI48163">
        <property type="protein sequence ID" value="CAI48163"/>
        <property type="gene ID" value="NP_0144A"/>
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    <dbReference type="GeneID" id="3700770"/>
    <dbReference type="KEGG" id="nph:NP_0144A"/>
    <dbReference type="eggNOG" id="arCOG02358">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_71_2"/>
    <dbReference type="OrthoDB" id="106630at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
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    </dbReference>
    <dbReference type="GO" id="GO:0000155">
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000006">
        <property type="entry name" value="Sensor histidine kinase WalK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
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        <property type="entry name" value="HATPase_C_sf"/>
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        <property type="entry name" value="HATPase_dom"/>
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    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
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    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
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    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
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    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
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    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013656">
        <property type="entry name" value="PAS_4"/>
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    <dbReference type="InterPro" id="IPR052162">
        <property type="entry name" value="Sensor_kinase/Photoreceptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
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        <property type="entry name" value="sensory_box"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR43304:SF1">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43304">
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    <dbReference type="Pfam" id="PF02518">
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
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            <begin position="411"/>
            <end position="624"/>
        </location>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50113"/>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI48163.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="628" mass="69550" checksum="0350EB11814D6D34" modified="2017-05-10" version="1"> </sequence>
</entry>
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        </recommendedName>
    </protein>
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    <organism evidence="2 3">
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
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            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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    <dbReference type="EnsemblBacteria" id="CAI48181">
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        <property type="gene ID" value="NP_0180A"/>
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    <dbReference type="HOGENOM" id="CLU_1965627_0_0_2"/>
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    <dbReference type="Proteomes" id="UP000002698">
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    </dbReference>
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        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="127" mass="13624" checksum="FA648E62806B7C22" modified="2017-05-10" version="1">MRDRVAVAVFLLAVVAAAVGGSLLTTDAPPSAAPNADFAVTGDPADTVTVEHVGGDSLDSGAVRVLVYEDRPLIPDRTVHATTWEEPGLIQETDRLEVEDPRFESGQRLVVRWFGDEGPATLYETHL</sequence>
</entry>
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    <accession>A0A1U7ETL5</accession>
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        <name evidence="5" type="ordered locus">NP_0314A</name>
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    <organism evidence="5 6">
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    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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                <person name="Pfeiffer F."/>
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            <dbReference type="PubMed" id="16169924"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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        <property type="gene ID" value="NP_0314A"/>
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    <dbReference type="HOGENOM" id="CLU_043913_0_0_2"/>
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    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010212">
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031657">
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    </dbReference>
    <dbReference type="InterPro" id="IPR051231">
        <property type="entry name" value="SOSS-B"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005551">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13356:SF10">
        <property type="entry name" value="REPLICATION FACTOR-A PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16900">
        <property type="entry name" value="REPA_OB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="88"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="OB" evidence="3">
        <location>
            <begin position="175"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Replication protein A OB" evidence="4">
        <location>
            <begin position="272"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="367"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="384"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="400"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI48248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="467" mass="50046" checksum="E3AB8E3E8F534B86" modified="2017-05-10" version="1">MGAIEDIYEDLDADVSESEFREAVEAKVEQMGGLADEETAAMLIAHDLDGGEVETIADIEPGLEEVKFLGKVASVGELRTFERDGEDDEDGHVLNLDVADESGTVRVALWDDDAVAADEEVEPGQVFRIKGRPKDGYSGVEVSADRIEADEEAEIEVDLDGRTTADSLSMGQSDVTLRGVVLDTDAVRTFDRDDGSEGRVSNLTLGDETGKIRVTLWDEQADRAEALEAGQSVEIVDGYVREREGDLELHAGSRSDIDELDESVEFVPDATDIDSIAIDETVDIAGVVRSADPKRTFDRDDGSEGQVRNVRLQDNTGDIRVALWGEKADLDVGPGDEVFCADVEIQDGWQDDLEASAGWQSTVVVLDDDDPDRGGDSAGLDEFSGGSAAAHDGSDGSDPAADDEAASDAGDEPETVTGTVVQPADPVILDNGDETIHVETDAEVHLGQEVTVRGRRDGERFVAEDVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETU0</accession>
    <name>A0A1U7ETU0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Response regulator CheY</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cheY2</name>
        <name evidence="3" type="ordered locus">NP_0516A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321985.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETU0"/>
    <dbReference type="STRING" id="348780.NP_0516A"/>
    <dbReference type="EnsemblBacteria" id="CAI48349">
        <property type="protein sequence ID" value="CAI48349"/>
        <property type="gene ID" value="NP_0516A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700881"/>
    <dbReference type="KEGG" id="nph:NP_0516A"/>
    <dbReference type="eggNOG" id="arCOG02391">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_15_2"/>
    <dbReference type="OrthoDB" id="2830at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052048">
        <property type="entry name" value="ST_Response_Regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43228">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43228:SF1">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR ARR22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="4"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="120" mass="13238" checksum="DE2E32C86F286658" modified="2017-05-10" version="1">MTQRVLVVDDSSLQRTIITGALGDRFEVVGEAENGQEAVELFEEKEPDLVTMDIMMPEMDGVEATGEIKNRTDDTKVMMCTSVEQAEKMKEAVRAGADEYVTKPFDEDELLDTATTLVGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETY2</accession>
    <name>A0A1U7ETY2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0616A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322035.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETY2"/>
    <dbReference type="STRING" id="348780.NP_0616A"/>
    <dbReference type="EnsemblBacteria" id="CAI48399">
        <property type="protein sequence ID" value="CAI48399"/>
        <property type="gene ID" value="NP_0616A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700906"/>
    <dbReference type="KEGG" id="nph:NP_0616A"/>
    <dbReference type="eggNOG" id="ENOG502N5HC">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2447752_0_0_2"/>
    <dbReference type="OrthoDB" id="383202at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="89" mass="9563" checksum="81E4A8F15AD6D3ED" modified="2017-05-10" version="1">MSYAKQDTAPPTTRYAKSTTAVVVGWLAAYAAYRLAPTWSRLDIDELVPAFLTFGLVIGFGFALGVYGLFRGIETAVAAGRVRTADDVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU76</accession>
    <name>A0A1U7EU76_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DsrE domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0846A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322150.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU76"/>
    <dbReference type="STRING" id="348780.NP_0846A"/>
    <dbReference type="EnsemblBacteria" id="CAI48514">
        <property type="protein sequence ID" value="CAI48514"/>
        <property type="gene ID" value="NP_0846A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700973"/>
    <dbReference type="KEGG" id="nph:NP_0846A"/>
    <dbReference type="eggNOG" id="arCOG01670">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_167520_0_0_2"/>
    <dbReference type="OrthoDB" id="122708at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="117" mass="12915" checksum="FF9A44682DB0C477" modified="2017-05-10" version="1">MTKAAIVILAGTEHMSDTGRLVNGLEAAREFREAGDEIELIFDGAATQWVGELESEDHPYHDLYLSVKDEIEVCEYCVGAFEQEAAVSEAGVGRRDEHDGHPSIRSLVEDGYEVITF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUC7</accession>
    <name>A0A1U7EUC7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0950A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48566.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322202.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUC7"/>
    <dbReference type="EnsemblBacteria" id="CAI48566">
        <property type="protein sequence ID" value="CAI48566"/>
        <property type="gene ID" value="NP_0950A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701008"/>
    <dbReference type="KEGG" id="nph:NP_0950A"/>
    <dbReference type="eggNOG" id="arCOG11189">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1754755_0_0_2"/>
    <dbReference type="OrthoDB" id="234678at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48566.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="148" mass="15498" checksum="F502D92302056189" modified="2017-05-10" version="1">MPTRRRVLAVAAAATATAGCTALGDGENDETGDADSVRLEDLSVQNNHDEDHQIQLAVEADGDVLHLGTYELDADGGSETVDGEWSDDTDSYRVHARLDDGAIQTADVTEGVGEGTDCVRVLVRVDGTGDLAVWNGANCGDDADPENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUF9</accession>
    <name>A0A1U7EUF9_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">A-type ATP synthase subunit C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">atpC</name>
        <name evidence="7" type="ordered locus">NP_1026A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Has multiple subunits with at least A(3), B(3), C, D, E, F, H, I and proteolipid K(x).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the V-ATPase V0D/AC39 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48604.1"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PRK01198.1-1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006709"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
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            <dbReference type="EMBL" id="CAI48604.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="353" mass="39866" checksum="64451E2310555BC4" modified="2017-05-10" version="1">MNVETGGSSNYEYVIARVRSRRAKLFDEDDYRKLVRMGPGEIARFMEETEYETEMNALGTRHSGVDLIEYALNRNQSKHFGDLLTWADGSLYNYIANYLRKFDAWNVKTAIRGIYTGADSEDIEDDLIRTGEFSEQTLDRLLEATSMEELVEQLSGTIFGEPLEEALEEYEDQGVLVPLENAVDRAFFSHLLDDIGSVEPDSPKALYREFLEAEIDFRNLRNGLRIARSGADIDPSEYFIEGGQLFGPDELRQLAANTDELVARIRDSAYGDDLASALNELESADSLIQFEHALDAALLEYSGKLSNRYPLSVCPILAYILAKEREVENIRAIARGREAGLSVEEIENELVIQ</sequence>
</entry>
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    <accession>A0A1U7EUN7</accession>
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        <name evidence="1" type="ordered locus">NP_1212A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <lineage>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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    </dbReference>
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            <dbReference type="EMBL" id="CAI48697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="40" mass="4734" checksum="CC3E895D65BCA43F" modified="2017-05-10" version="1">MVLHTMTPNRFQERLEETRGRVDTDEVVAALSYVREDGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUT9</accession>
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    </protein>
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        <name evidence="1" type="ordered locus">NP_1300A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
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        <lineage>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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            <authorList>
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                <person name="Pfeiffer F."/>
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                <person name="Rodewald K."/>
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            </authorList>
            <dbReference type="PubMed" id="16169924"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="152" mass="16102" checksum="01285DDDA3AB28A5" modified="2017-05-10" version="1">MLSADELAGVVDLFGALSRPELSEALSELAYRRGEDPPEEIVEDAVAAFALVGFVDDGHRLFAPGPAAFPTLPEGAEDLPHILDQQPRSVDRAAVAEAAADRLRTEAACAATLGATDRAERLVDISYDLETWAALDLSGVRSRLDATLDGTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUY6</accession>
    <name>A0A1U7EUY6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ordered locus">NP_1446A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        </lineage>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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            <authorList>
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAI48814"/>
        <property type="gene ID" value="NP_1446A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701153"/>
    <dbReference type="KEGG" id="nph:NP_1446A"/>
    <dbReference type="eggNOG" id="arCOG08912">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="319931at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="83" mass="8740" checksum="2313A7C2E909FFAA" modified="2017-05-10" version="1">MDDIVATVRETLEADGYTVNSAGVNRGQVRVSLLEADASAAALRELVTDAVGESAVVGLSVANEAPEGEDDVRTVITFRYRDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV37</accession>
    <name>A0A1U7EV37_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Fe-S cluster assembly ATPase SufC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">sufC</name>
        <name evidence="5" type="synonym">abc31a</name>
        <name evidence="5" type="ordered locus">NP_1504A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48843.1"/>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
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    <dbReference type="STRING" id="348780.NP_1504A"/>
    <dbReference type="EnsemblBacteria" id="CAI48843">
        <property type="protein sequence ID" value="CAI48843"/>
        <property type="gene ID" value="NP_1504A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702456"/>
    <dbReference type="KEGG" id="nph:NP_1504A"/>
    <dbReference type="eggNOG" id="arCOG04236">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_48_1_2"/>
    <dbReference type="OrthoDB" id="18492at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03217">
        <property type="entry name" value="ABC_FeS_Assembly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010230">
        <property type="entry name" value="FeS-cluster_ATPase_SufC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43204">
        <property type="entry name" value="ABC TRANSPORTER I FAMILY MEMBER 6, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43204:SF1">
        <property type="entry name" value="ABC TRANSPORTER I FAMILY MEMBER 6, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="132"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="132"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI48843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="302" mass="33503" checksum="CFE6266CA1C18140" modified="2017-05-10" version="1">MATLEINNLHAKVAEDGGERILRGVDLEVASGEIHALMGPNGSGKSTTAKVIAGHPAYEVTEGEVLLHVEEDEFDDIDIDDDQLTWDLLDLEPNERAALGIFLAFQYPAEIEGVTMVNFLRTALNAKLEEREELFEEEEDSEDEDDEAGYENSPMEGPADEGEVGVAEFQEILQEKMELLDMDESFAQRYLNAGFSGGEKKQNEVLQAAILEPSIAVLDEIDSGLDIDRLQDVSDGINALRDEQGTGILQITHYQRILDYVEPDHVHVMLDGEIAKSGDASLAEELEDKGYDWVREEVYETA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV50</accession>
    <name>A0A1U7EV50_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphodiesterase domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1610A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322530.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV50"/>
    <dbReference type="STRING" id="348780.NP_1610A"/>
    <dbReference type="EnsemblBacteria" id="CAI48896">
        <property type="protein sequence ID" value="CAI48896"/>
        <property type="gene ID" value="NP_1610A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701200"/>
    <dbReference type="KEGG" id="nph:NP_1610A"/>
    <dbReference type="eggNOG" id="arCOG14836">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1431683_0_0_2"/>
    <dbReference type="OrthoDB" id="378816at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009097">
        <property type="entry name" value="Cyclic_Pdiesterase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55144">
        <property type="entry name" value="LigT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="21288" checksum="265FC03DC86DE7FB" modified="2017-05-10" version="1">MPTRYVTGWISRPIVGPNRVLTLRDHLYGWLGAPDPYRRADEPHVSLFGIRLPEQQRDAFEKRLDSFGAAVGSWTGTVDGYRFYPSLKNPMVVSLDVPVPQARVASPVADLVGAFDGVVDSRPTPPHVTLLMGGVRGEELQWAQVDETVRRRLRAVTGEAVSDRTRHTGSSPPEPLVDPAFPVTVGPPEVEWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV90</accession>
    <name>A0A1U7EV90_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1664A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322556.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV90"/>
    <dbReference type="STRING" id="348780.NP_1664A"/>
    <dbReference type="EnsemblBacteria" id="CAI48923">
        <property type="protein sequence ID" value="CAI48923"/>
        <property type="gene ID" value="NP_1664A"/>
    </dbReference>
    <dbReference type="GeneID" id="54763311"/>
    <dbReference type="KEGG" id="nph:NP_1664A"/>
    <dbReference type="eggNOG" id="arCOG06374">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_210687_0_0_2"/>
    <dbReference type="OrthoDB" id="211218at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="50" mass="5714" checksum="8AD45469CFD5FE0F" modified="2017-05-10" version="1">MTDTDDAIETFLAESETVLEEYDQGYADADATLRQLRQHIETLREAHDDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVB6</accession>
    <name>A0A1U7EVB6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dihydroorotase</fullName>
            <shortName evidence="4">DHOase</shortName>
            <ecNumber evidence="4">3.5.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 6" type="primary">pyrC</name>
        <name evidence="6" type="ordered locus">NP_1706A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the reversible cyclization of carbamoyl aspartate to dihydroorotate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24296"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="EC" id="3.5.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the metallo-dependent hydrolases superfamily. DHOase family. Class I DHOase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.3" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322577.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVB6"/>
    <dbReference type="STRING" id="348780.NP_1706A"/>
    <dbReference type="EnsemblBacteria" id="CAI48944">
        <property type="protein sequence ID" value="CAI48944"/>
        <property type="gene ID" value="NP_1706A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703082"/>
    <dbReference type="KEGG" id="nph:NP_1706A"/>
    <dbReference type="eggNOG" id="arCOG00689">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015572_1_1_2"/>
    <dbReference type="OrthoDB" id="50279at2157"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00117"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004038">
        <property type="term" value="F:allantoinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004151">
        <property type="term" value="F:dihydroorotase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006145">
        <property type="term" value="P:purine nucleobase catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01318">
        <property type="entry name" value="DHOase_IIb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00220_A">
        <property type="entry name" value="PyrC_classI_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004722">
        <property type="entry name" value="DHOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050138">
        <property type="entry name" value="DHOase/Allantoinase_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002195">
        <property type="entry name" value="Dihydroorotase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002668">
        <property type="entry name" value="PRK02382.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00857">
        <property type="entry name" value="pyrC_multi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43668">
        <property type="entry name" value="ALLANTOINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43668:SF2">
        <property type="entry name" value="ALLANTOINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00482">
        <property type="entry name" value="DIHYDROOROTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00483">
        <property type="entry name" value="DIHYDROOROTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 4" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="5">
        <location>
            <begin position="44"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="289"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="54"/>
            <end position="56"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI48944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="422" mass="45402" checksum="0AE0DAD9A557BA44" modified="2017-05-10" version="1">MLFSNATLADGRVRDVRVDGETIAEVGTGLEADGEVVDAEGKRLLPGMIDAHVHFRQPGYSHKETWETGSKSAAAGGVTAVFDQPNTDPPTIDADGYAEKASLATASLVDYGINGGVTENWSPTELLEEPIAALGEVFLADSTGDMGIGRERFAEALDHATDAGVLVTVHAEDATRFDESATERDNADAWSAYRTAAAEIDAIERACETADEHGADIHIAHTSTPEGADIAREAGMTCEVTPHHLFLSRDDLEELGTFGRMNPPLRSETRREAMFERLVDGDIDIVATDHAPHTRAEKDASIWDAPSGVPGVETVLPLLLDEARKGRISYERLRDVTAANVADIFDLPDKGRIEAGNDADLVVVDPDDAEPIRGDNLNSKCEWTPFEGRDGVFPELTMVRGTVVYRDGEFADAVGHGQNVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVC6</accession>
    <name>A0A1U7EVC6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1760A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322604.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVC6"/>
    <dbReference type="STRING" id="348780.NP_1760A"/>
    <dbReference type="EnsemblBacteria" id="CAI48971">
        <property type="protein sequence ID" value="CAI48971"/>
        <property type="gene ID" value="NP_1760A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701247"/>
    <dbReference type="KEGG" id="nph:NP_1760A"/>
    <dbReference type="eggNOG" id="arCOG11366">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2766216_0_0_2"/>
    <dbReference type="OrthoDB" id="228288at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="69" mass="7591" checksum="59B3DA0BC25E4600" modified="2017-05-10" version="1">MSDDYDIDPEFLYPSDADVLDISAGDDGKVHVRFVVPSPDTGDALELDAVVESIEESDTELPLDDELYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVD5</accession>
    <name>A0A1U7EVD5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC-type transport system permease protein (Probable substrate glutamine/glutamate/polar amino acids)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">abc04p2b</name>
        <name evidence="10" type="ordered locus">NP_1780A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322614.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVD5"/>
    <dbReference type="STRING" id="348780.NP_1780A"/>
    <dbReference type="EnsemblBacteria" id="CAI48981">
        <property type="protein sequence ID" value="CAI48981"/>
        <property type="gene ID" value="NP_1780A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702376"/>
    <dbReference type="KEGG" id="nph:NP_1780A"/>
    <dbReference type="eggNOG" id="arCOG01798">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019602_1_0_2"/>
    <dbReference type="OrthoDB" id="60458at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010065">
        <property type="entry name" value="AA_ABC_transptr_permease_3TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043429">
        <property type="entry name" value="ArtM/GltK/GlnP/TcyL/YhdX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01726">
        <property type="entry name" value="HEQRo_perm_3TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30614:SF0">
        <property type="entry name" value="L-CYSTINE TRANSPORT SYSTEM PERMEASE PROTEIN TCYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30614">
        <property type="entry name" value="MEMBRANE COMPONENT OF AMINO ACID ABC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="109"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="251"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="283"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="105"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI48981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="325" mass="35267" checksum="097180B0CEF0EB69" modified="2017-05-10" version="1">MTSRDDALGTSSLSSTGRTALLVVGIAFWGWLLTRWAYDWVINPGVGVSSREPFVPAGPFEAAGGWFEGLAADAGVLSTPLGWLAWLFDFIAFGVTVMPALAAGAWLTIVLTVLGMFFGIFIAVPLSVARVYGSRPLRWLSLAYTELIRGTPLLAQLFVLYYGLPLSGIIRELPGVGVGPVPRAAVWVAVIGFTINSSAYQAEYIRSALQSVDAGQLTAARSVGLSKTESIRHVILPQGLRYAIPGWTNEFIYLIKYSSLAAFIAVPELFRRARNIGSDTFRYLEMYLIVALFYLALVLTLSVLMDSFEKRVAIPGLGTAADDRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVH0</accession>
    <name>A0A1U7EVH0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1109 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1852A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49017.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939486.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVH0"/>
    <dbReference type="EnsemblBacteria" id="CAI49017">
        <property type="protein sequence ID" value="CAI49017"/>
        <property type="gene ID" value="NP_1852A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701277"/>
    <dbReference type="KEGG" id="nph:NP_1852A"/>
    <dbReference type="eggNOG" id="arCOG06332">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_989038_0_0_2"/>
    <dbReference type="OrthoDB" id="236970at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="10"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49017.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="281" mass="29997" checksum="E0776A5FC4BE4258" modified="2017-05-10" version="1">MNFNIDGGKLLYVLGVLFAFAALLYFVSDVVFGLSITVTALLLFVAFVAFLVAGLAIDRDALDVVAYAISGLSYAVFLGYVTARYGLGETGIFLLLAASAGLFVGLGYGVRQQSLDIDRRTAAYVAVVLVAVGVVFIGADLATGDVEYTVEMQDETTISVTDEHVERDRPRVTADLWTVTATNPSPFTRPVDLPSARGCLSGVAEFGDETFPVRYEPRSFGTPDRLDGGAERTHTLTGSVPVVADDPGERTYAVERAESCDVSHDEPTIVVVFEDDTAVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVH5</accession>
    <name>A0A1U7EVH5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Gamma-glutamyltransferase</fullName>
            <ecNumber evidence="1">2.3.2.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ggt</name>
        <name evidence="1" type="ordered locus">NP_1844A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.2.2" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVH5"/>
    <dbReference type="STRING" id="348780.NP_1844A"/>
    <dbReference type="EnsemblBacteria" id="CAI49013">
        <property type="protein sequence ID" value="CAI49013"/>
        <property type="gene ID" value="NP_1844A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_1844A"/>
    <dbReference type="eggNOG" id="arCOG04053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014813_3_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036374">
        <property type="term" value="F:glutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103068">
        <property type="term" value="F:leukotriene C4 gamma-glutamyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052896">
        <property type="entry name" value="GGT-like_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043138">
        <property type="entry name" value="GGT_lsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000101">
        <property type="entry name" value="GGT_peptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043137">
        <property type="entry name" value="GGT_ssub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00066">
        <property type="entry name" value="g_glut_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43881:SF5">
        <property type="entry name" value="GAMMA-GLUTAMYLTRANSPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43881">
        <property type="entry name" value="GAMMA-GLUTAMYLTRANSPEPTIDASE (AFU_ORTHOLOGUE AFUA_4G13580)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01019">
        <property type="entry name" value="G_glu_transpept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01210">
        <property type="entry name" value="GGTRANSPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="574" mass="61233" checksum="57B9A072EC0BB27C" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVH7</accession>
    <name>A0A1U7EVH7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transducer protein Htr26</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">htr26</name>
        <name evidence="9" type="ordered locus">NP_1860A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322653.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVH7"/>
    <dbReference type="STRING" id="348780.NP_1860A"/>
    <dbReference type="EnsemblBacteria" id="CAI49021">
        <property type="protein sequence ID" value="CAI49021"/>
        <property type="gene ID" value="NP_1860A"/>
    </dbReference>
    <dbReference type="GeneID" id="32154996"/>
    <dbReference type="KEGG" id="nph:NP_1860A"/>
    <dbReference type="eggNOG" id="arCOG02318">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_21_2_2"/>
    <dbReference type="OrthoDB" id="8523at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01068">
        <property type="entry name" value="globin_sensor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11386">
        <property type="entry name" value="MCP_signal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="entry name" value="Globins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.950">
        <property type="entry name" value="Methyl-accepting chemotaxis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004090">
        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044398">
        <property type="entry name" value="Globin-sensor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013656">
        <property type="entry name" value="PAS_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039379">
        <property type="entry name" value="Protoglobin_sensor_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32089:SF112">
        <property type="entry name" value="LYSOZYME-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32089">
        <property type="entry name" value="METHYL-ACCEPTING CHEMOTAXIS PROTEIN MCPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08448">
        <property type="entry name" value="PAS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11563">
        <property type="entry name" value="Protoglobin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00260">
        <property type="entry name" value="CHEMTRNSDUCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="Globin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58104">
        <property type="entry name" value="Methyl-accepting chemotaxis protein (MCP) signaling domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="PAS" evidence="7">
        <location>
            <begin position="246"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="8">
        <location>
            <begin position="321"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="6">
        <location>
            <begin position="384"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="14"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="361"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="525"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="14"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI49021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="661" mass="72459" checksum="3A1B76EF91F623BB" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVI0</accession>
    <name>A0A1U7EVI0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DUF1628 domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_1876A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322661.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVI0"/>
    <dbReference type="STRING" id="348780.NP_1876A"/>
    <dbReference type="EnsemblBacteria" id="CAI49029">
        <property type="protein sequence ID" value="CAI49029"/>
        <property type="gene ID" value="NP_1876A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701286"/>
    <dbReference type="KEGG" id="nph:NP_1876A"/>
    <dbReference type="eggNOG" id="arCOG02416">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1096737_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013373">
        <property type="entry name" value="Flagellin/pilin_N_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012859">
        <property type="entry name" value="Pilin_N_archaeal"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02537">
        <property type="entry name" value="arch_flag_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07790">
        <property type="entry name" value="Pilin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Archaeal Type IV pilin N-terminal" evidence="2">
        <location>
            <begin position="10"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="253" mass="26958" checksum="BD82AFAA1BA3A60A" modified="2017-05-10" version="1">MVPERRGAVPVTGVAFLVAITLILATTASVFVFGIGDDGIADEAVPSAAFDTATESTTLTLTHESGDTIDGDRLEVQGGVNTDVPETVAAGSRIETTPIEDEVRLVWHGDDISATLTTVETDSVDLSELSAFVIESFAVDDSDCPSGTANIDATIRNTGEVTHEQDIKLYLVYDDGEDELFETRTLELAPGETEEISETRSCNEFEDNPDAVRLETEDDEAVEDLAGFGVLVALIAIGLALVRFSRLGRSKNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVJ3</accession>
    <name>A0A1U7EVJ3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF457 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1862A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322654.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVJ3"/>
    <dbReference type="EnsemblBacteria" id="CAI49022">
        <property type="protein sequence ID" value="CAI49022"/>
        <property type="gene ID" value="NP_1862A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701280"/>
    <dbReference type="KEGG" id="nph:NP_1862A"/>
    <dbReference type="eggNOG" id="arCOG04664">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1357894_0_0_2"/>
    <dbReference type="OrthoDB" id="204671at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="205" mass="21792" checksum="F18906B8D8CCEF4F" modified="2017-05-10" version="1">MMLPTHVLSGMALALPLLVVAPEFAPVGLVAGFLGGLAPDLDMYFGHRKTLHYPVYYSVLAAVAVPVTLAAPSTVTIAMALFFLGAALHCVADVFGGGLELRPWEGTSERAVYDHLRGEWIAPRRTVGYDGSPADLLLMGVFAVPLLAVLDGPLWLVVLGTALVGAVYTLTRRHLAALAPIVVRALPSTVHPYVPARYRSEGRGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVK1</accession>
    <name>A0A1U7EVK1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Peptidase C26 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1950A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322696.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVK1"/>
    <dbReference type="STRING" id="348780.NP_1950A"/>
    <dbReference type="EnsemblBacteria" id="CAI49066">
        <property type="protein sequence ID" value="CAI49066"/>
        <property type="gene ID" value="NP_1950A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701316"/>
    <dbReference type="KEGG" id="nph:NP_1950A"/>
    <dbReference type="eggNOG" id="arCOG00088">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030756_5_0_2"/>
    <dbReference type="OrthoDB" id="3321at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01745">
        <property type="entry name" value="GATase1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011697">
        <property type="entry name" value="Peptidase_C26"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07722">
        <property type="entry name" value="Peptidase_C26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="226" mass="25122" checksum="FC2B3657BBC88D55" modified="2017-05-10" version="1">MRRLGVTQRVEVVEEYGERRDCLDQAWTELLESWEFRPFPLPNTVENVETYLDSLNLDGIVLTSGNDLSNLKNPDDPAPERDRFERAALEWAIDHKVPVCGVCRGLELLNDHFDGSLSPVSGHVAVDHGVSFGYEAIDFSEDADTVQLPSEIKTNSYHDYGIDPSGVADPLSVLGTAADGTVEALHHPELPVLGIMWHPERDPDRPESSRALDRKLFDALFGESGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVL6</accession>
    <name>A0A1U7EVL6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Pilin PilA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pilA1</name>
        <name evidence="4" type="ordered locus">NP_1956A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322699.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVL6"/>
    <dbReference type="STRING" id="348780.NP_1956A"/>
    <dbReference type="EnsemblBacteria" id="CAI49069">
        <property type="protein sequence ID" value="CAI49069"/>
        <property type="gene ID" value="NP_1956A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701319"/>
    <dbReference type="KEGG" id="nph:NP_1956A"/>
    <dbReference type="eggNOG" id="arCOG02416">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116126_0_0_2"/>
    <dbReference type="OrthoDB" id="118020at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013373">
        <property type="entry name" value="Flagellin/pilin_N_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012859">
        <property type="entry name" value="Pilin_N_archaeal"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02537">
        <property type="entry name" value="arch_flag_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38138:SF1">
        <property type="entry name" value="ARCHAEAL TYPE IV PILIN N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38138">
        <property type="entry name" value="VNG6441H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07790">
        <property type="entry name" value="Pilin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="Archaeal Type IV pilin N-terminal" evidence="3">
        <location>
            <begin position="15"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="159" mass="16816" checksum="3F5D320F9FDAD4F3" modified="2017-05-10" version="1">MNLSNLLNPETDDRRAVSPVIGVILMVAITVILAAVIGTFVLGLGDQVQETAPNAQISADNGTDNGNIETTFTHESGDSINAENLFVTGSAVDGERIRADDEDLEQNVSDTMRAGGQIVLNSDGSPGSFDPSGSDDIRLVWQSQEGERTSTLASFEYGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVM4</accession>
    <name>A0A1U7EVM4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Probable glycosyltransferase, type 1</fullName>
            <ecNumber evidence="3">2.4.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">gth5</name>
        <name evidence="3" type="ordered locus">NP_1928A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.4.-.-" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322687.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVM4"/>
    <dbReference type="STRING" id="348780.NP_1928A"/>
    <dbReference type="EnsemblBacteria" id="CAI49055">
        <property type="protein sequence ID" value="CAI49055"/>
        <property type="gene ID" value="NP_1928A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701308"/>
    <dbReference type="KEGG" id="nph:NP_1928A"/>
    <dbReference type="eggNOG" id="arCOG01403">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_0_3_2"/>
    <dbReference type="OrthoDB" id="131038at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03801">
        <property type="entry name" value="GT4_PimA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050194">
        <property type="entry name" value="Glycosyltransferase_grp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45947">
        <property type="entry name" value="SULFOQUINOVOSYL TRANSFERASE SQD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45947:SF3">
        <property type="entry name" value="SULFOQUINOVOSYL TRANSFERASE SQD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase subfamily 4-like N-terminal" evidence="2">
        <location>
            <begin position="15"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="1">
        <location>
            <begin position="185"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="354" mass="39775" checksum="9FF2C3E027072A46" modified="2017-05-10" version="1">MRVAIILKTFPPDVIGGMETQTKRMATELHHQGHDVTVFTKRFGDHDDSDVPYEVVRIQNWRVNPFVSDLTFLVFAMLALIRRRNEFDVLQCMMLYPVGFLGYVLNRLTGLPYFAWVRGGDYYFMNDVWWKRWMMRRVLDDTLVLAQSGKIRDDVVADFDDIECNIDILGNAVSIPDETASGEGVLYVGRLAPKKGIEYLIDAVAEINASLTIVGDGSERDRLERRAAEAGADVTFEGEVDPDDVGRYYRSAGVFALASTEGEGMPNVVLEAMSWGLPVVATDSGGLPSLIDTGYNGYLVPMRDSQALSAKIERLLADDQHRANLGENARRFVADYHSWEALCSELKTHYERVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVM7</accession>
    <name>A0A1U7EVM7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Aspartate-semialdehyde dehydrogenase</fullName>
            <ecNumber evidence="6">1.2.1.11</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">asd</name>
        <name evidence="6" type="ordered locus">NP_1988A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aspartate-semialdehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.11" evidence="6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322715.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVM7"/>
    <dbReference type="STRING" id="348780.NP_1988A"/>
    <dbReference type="EnsemblBacteria" id="CAI49085">
        <property type="protein sequence ID" value="CAI49085"/>
        <property type="gene ID" value="NP_1988A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702531"/>
    <dbReference type="KEGG" id="nph:NP_1988A"/>
    <dbReference type="eggNOG" id="arCOG00494">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049966_1_0_2"/>
    <dbReference type="OrthoDB" id="38238at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004073">
        <property type="term" value="F:aspartate-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18130">
        <property type="entry name" value="ASADH_C_arch_fung_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02315">
        <property type="entry name" value="ScASADH_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051823">
        <property type="entry name" value="ASADH-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005676">
        <property type="entry name" value="Asp_semi-ald_DH_pep-lack"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012280">
        <property type="entry name" value="Semialdhyde_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00978">
        <property type="entry name" value="asd_EA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006416">
        <property type="entry name" value="PRK08664.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46718">
        <property type="entry name" value="ASPARTATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46718:SF1">
        <property type="entry name" value="ASPARTATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02774">
        <property type="entry name" value="Semialdhyde_dhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000148">
        <property type="entry name" value="ASA_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Semialdehyde dehydrogenase NAD-binding" evidence="5">
        <location>
            <begin position="5"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="4">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000148-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI49085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="345" mass="36503" checksum="BBAFC31A03AA49B2" modified="2017-05-10" version="1">MSTTRVGVLGATGAVGQRLIQLLDPHEEFEVAALSASSASAGKTYREAAKWRVDTPIPESVADMTVVETDPDAVPDDIDLLFSSLPSDIGADVEPEFVEAGYVVSSNSSNGRMDDDIPLTIPEVNADHLGLIEVQRDERGWDGALIKNPNCSTITMVPALAALDEFGLENIHVSTLQAVSGAGYSGVTSMEIIDNVIPHIGGEAEKMETESRKLLGSFDGAGVDLHEADVSASCNRVPTLDGHLENVWADLAADPDPADVVEAFESYPSVDLPSSPDQLIEVFDEPDRPQPRMDRMLGDGMAIATGGVESTADGIQFNCLAHNTMRGAAGASVLNGELLLEEGYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVQ8</accession>
    <name>A0A1U7EVQ8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2084A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322761.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVQ8"/>
    <dbReference type="EnsemblBacteria" id="CAI49133">
        <property type="protein sequence ID" value="CAI49133"/>
        <property type="gene ID" value="NP_2084A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701348"/>
    <dbReference type="KEGG" id="nph:NP_2084A"/>
    <dbReference type="eggNOG" id="arCOG07990">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_179007_0_0_2"/>
    <dbReference type="OrthoDB" id="194703at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="91" mass="10093" checksum="ACF5FA60CA7811F4" modified="2017-05-10" version="1">MATAVKIDDETKSKLEELQADIRRKTGSKVTQQAILSKLVGSAIDSRSEVVDSFRKTDRSLSETAIQQFNTGQIASGIETDEENIDDALYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVS9</accession>
    <name>A0A1U7EVS9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cro/C1 family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_2146A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322792.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVS9"/>
    <dbReference type="STRING" id="348780.NP_2146A"/>
    <dbReference type="EnsemblBacteria" id="CAI49164">
        <property type="protein sequence ID" value="CAI49164"/>
        <property type="gene ID" value="NP_2146A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701363"/>
    <dbReference type="KEGG" id="nph:NP_2146A"/>
    <dbReference type="eggNOG" id="arCOG01863">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130237_0_0_2"/>
    <dbReference type="OrthoDB" id="11138at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004451">
        <property type="entry name" value="MJ0586"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058562">
        <property type="entry name" value="MJ0586_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00270">
        <property type="entry name" value="multiprotein bridging factor aMBF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10245:SF15">
        <property type="entry name" value="ENDOTHELIAL DIFFERENTIATION-RELATED FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10245">
        <property type="entry name" value="ENDOTHELIAL DIFFERENTIATION-RELATED FACTOR 1 MULTIPROTEIN BRIDGING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26602">
        <property type="entry name" value="HVO_2718_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="3">
        <location>
            <begin position="100"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="35"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="129"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="42"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="96"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="139"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="162"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="181" mass="18868" checksum="5CF22BF97709AB0C" modified="2017-05-10" version="1">MVQCEMCGAETGSPKTVKIEGAELDVCDDCADFGTEVQTQDTSSTSTKYSTSSSGSSSSGGSSGSSTSSSTGSGGGGGGGSRRDMFDEMDEVVQDYDDRIREAREAASMTQEELANSLNEKASLIRKLERGEVLPSDSVQRKLERELDISLSTGGGSDDNDWSGGSSTGKTTLGDVVKRKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVU2</accession>
    <name>A0A1U7EVU2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Taxis protein CheF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cheF2</name>
        <name evidence="2" type="ordered locus">NP_2162A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in taxis signal transduction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with chemotaxis (Che) proteins as well as flagella accessory (Fla) proteins.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322800.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVU2"/>
    <dbReference type="STRING" id="348780.NP_2162A"/>
    <dbReference type="EnsemblBacteria" id="CAI49172">
        <property type="protein sequence ID" value="CAI49172"/>
        <property type="gene ID" value="NP_2162A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701367"/>
    <dbReference type="KEGG" id="nph:NP_2162A"/>
    <dbReference type="eggNOG" id="arCOG02394">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063179_0_0_2"/>
    <dbReference type="OrthoDB" id="337296at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007381">
        <property type="entry name" value="CheF1/F2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42201">
        <property type="entry name" value="TAXIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42201:SF1">
        <property type="entry name" value="TAXIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04283">
        <property type="entry name" value="CheF-arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF026802">
        <property type="entry name" value="UCP026802"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR026802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="293" mass="31861" checksum="FD1F1A4CD891DCBF" modified="2017-05-10" version="1">MKEEAVADFVGRFARNPEGGVQGEPQSCRAVMSKKRLVVAGDGDERITVPLSRVVDVVVGNVPPNLRDLFDATVTVGYKTDEGTVETVLIEGDDSTMSKFQTVLFKCLLNGTKALVKHPAQLGGRVTDSPVRKAKISIESKQVRFKTSGDNFTIDITNVIDFERTERAPDGESRPTLVVKHADDGQVATSLVSPASSRKLNLLGRYFRIEYSELLNEVGQIDLSESEKRVLVTIYATGGDIDFKNVLDGSAAQATNVVNSLREKGLIEEEPTGLSLTSHGQVVVSQRLEDVNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVV6</accession>
    <name>A0A1U7EVV6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DUF35 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2216A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322826.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVV6"/>
    <dbReference type="STRING" id="348780.NP_2216A"/>
    <dbReference type="EnsemblBacteria" id="CAI49199">
        <property type="protein sequence ID" value="CAI49199"/>
        <property type="gene ID" value="NP_2216A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701379"/>
    <dbReference type="KEGG" id="nph:NP_2216A"/>
    <dbReference type="eggNOG" id="arCOG01285">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1922879_0_0_2"/>
    <dbReference type="OrthoDB" id="9573at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.30.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002878">
        <property type="entry name" value="ChsH2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022002">
        <property type="entry name" value="ChsH2_Znr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052513">
        <property type="entry name" value="Thioester_dehydratase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34075">
        <property type="entry name" value="BLR3430 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34075:SF5">
        <property type="entry name" value="BLR3430 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01796">
        <property type="entry name" value="OB_ChsH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12172">
        <property type="entry name" value="zf-ChsH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ChsH2 rubredoxin-like zinc ribbon" evidence="2">
        <location>
            <begin position="11"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="ChsH2 C-terminal OB-fold" evidence="1">
        <location>
            <begin position="48"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="121" mass="12789" checksum="CFC791E314E4BF19" modified="2017-05-10" version="1">MSDLTHNEWVSAVKDGELLGQTCPDCGATHGTPKAACPHCGSRDLETVALPTEGTVYTETTINVPPINIDERGYQVAVVAVGEARVMGRLVEEDVDIGDSVAVAGYDEDDTGNVTPRFEAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVW1</accession>
    <name>A0A1U7EVW1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">3-dehydroquinate dehydratase</fullName>
            <shortName evidence="4">3-dehydroquinase</shortName>
            <ecNumber evidence="4">4.2.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Type I DHQase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Type I dehydroquinase</fullName>
            <shortName evidence="4">DHQ1</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">aroD</name>
        <name evidence="5" type="ordered locus">NP_2240A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in the third step of the chorismate pathway, which leads to the biosynthesis of aromatic amino acids. Catalyzes the cis-dehydration of 3-dehydroquinate (DHQ) and introduces the first double bond of the aromatic ring to yield 3-dehydroshikimate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>3-dehydroquinate = 3-dehydroshikimate + H2O</text>
            <dbReference type="Rhea" id="RHEA:21096"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="EC" id="4.2.1.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the type-I 3-dehydroquinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.10" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322838.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVW1"/>
    <dbReference type="STRING" id="348780.NP_2240A"/>
    <dbReference type="EnsemblBacteria" id="CAI49211">
        <property type="protein sequence ID" value="CAI49211"/>
        <property type="gene ID" value="NP_2240A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702528"/>
    <dbReference type="KEGG" id="nph:NP_2240A"/>
    <dbReference type="eggNOG" id="arCOG02097">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064444_1_0_2"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00086"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003855">
        <property type="term" value="F:3-dehydroquinate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046279">
        <property type="term" value="P:3,4-dihydroxybenzoate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00502">
        <property type="entry name" value="DHQase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00214">
        <property type="entry name" value="AroD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001381">
        <property type="entry name" value="DHquinase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050146">
        <property type="entry name" value="Type-I_3-dehydroquinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43699">
        <property type="entry name" value="3-DEHYDROQUINATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43699:SF1">
        <property type="entry name" value="3-DEHYDROQUINATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01487">
        <property type="entry name" value="DHquinase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="2 4 5" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0704">Schiff base</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="4">
        <location>
            <position position="119"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="4">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="31"/>
            <end position="33"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="225" mass="23297" checksum="1D6400A695990D20" modified="2017-05-10" version="1">MAIPFETFTLAAATADLGDEPAAREHADCVEFRMDLAAEPRSALGSYDGELPLLVTNRPTWEGGEADDEGRLPALEAAVEHDAVGAVDIELAALEDNAAARSLADHAHGNDASVVVSTHDFEATPARSTLVDLLERAGTYGDVAKLAVTATDRSDALDVLAATHEATQKGTTVATMAMGEAGSHTRAVAPVYGSQIGYAPVDPAEATAPGQYDLATLRELVSSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVW6</accession>
    <name>A0A1U7EVW6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Probable rhomboid family protease</fullName>
            <ecNumber evidence="7">3.4.21.105</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">NP_2230A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.21.105" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322833.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVW6"/>
    <dbReference type="EnsemblBacteria" id="CAI49206">
        <property type="protein sequence ID" value="CAI49206"/>
        <property type="gene ID" value="NP_2230A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701384"/>
    <dbReference type="KEGG" id="nph:NP_2230A"/>
    <dbReference type="eggNOG" id="arCOG01771">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037598_0_0_2"/>
    <dbReference type="OrthoDB" id="205691at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="83"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="106"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="143"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="221"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="257"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="287"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="324"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="6">
        <location>
            <begin position="89"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="548" mass="58359" checksum="D7657ECC642C98B9" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVY1</accession>
    <name>A0A1U7EVY1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2264A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322850.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVY1"/>
    <dbReference type="STRING" id="348780.NP_2264A"/>
    <dbReference type="EnsemblBacteria" id="CAI49223">
        <property type="protein sequence ID" value="CAI49223"/>
        <property type="gene ID" value="NP_2264A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701395"/>
    <dbReference type="KEGG" id="nph:NP_2264A"/>
    <dbReference type="HOGENOM" id="CLU_193456_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058341">
        <property type="entry name" value="DUF8028"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26071">
        <property type="entry name" value="DUF8028"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="83" mass="8346" checksum="FD9A1D5EEB470F5B" modified="2017-05-10" version="1">MSSATPPRSTVHSPELQTALSALGRQLAAVVRAAAFWLAALLPLAVLAALVVGSLTQSPALLAGVLTLNVVCAVVGHGHSPEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW10</accession>
    <name>A0A1U7EW10_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Resolvase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_2354A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49268.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939817.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW10"/>
    <dbReference type="STRING" id="348780.NP_2354A"/>
    <dbReference type="EnsemblBacteria" id="CAI49268">
        <property type="protein sequence ID" value="CAI49268"/>
        <property type="gene ID" value="NP_2354A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703274"/>
    <dbReference type="KEGG" id="nph:NP_2354A"/>
    <dbReference type="eggNOG" id="arCOG03162">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010686_7_0_2"/>
    <dbReference type="OrthoDB" id="24728at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03768">
        <property type="entry name" value="SR_ResInv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="entry name" value="Resolvase, N-terminal catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006118">
        <property type="entry name" value="Recombinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050639">
        <property type="entry name" value="SSR_resolvase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30461">
        <property type="entry name" value="DNA-INVERTASE FROM LAMBDOID PROPHAGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30461:SF26">
        <property type="entry name" value="RESOLVASE HOMOLOG YNEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="Resolvase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00397">
        <property type="entry name" value="RECOMBINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="4">
        <location>
            <begin position="2"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49268.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="217" mass="24331" checksum="B628A4D477CFE675" modified="2017-05-10" version="1">MTVACYVRVSTADQNLDRQLESTSNYAQDELGAALGDIDTYRDKSTGTDTERRGYRDLVAQLEAGTVDAVVVHEISRLARSLQDLERTVSRVTDAGAEIHFVRDGLVFGDGKEKPMQRLQLQMLGAFAEWEARVKQMNTREGIAARQQNDEYHHGPAPLGFEKDDGRLIETDTYHEVVAVLDMVQKDELSKRKAAGRLDCSRSTIGRALDRRELYGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW15</accession>
    <name>A0A1U7EW15_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Homolog to DNA mismatch repair protein MutH</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">mutH</name>
        <name evidence="6" type="ordered locus">NP_2356A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW15"/>
    <dbReference type="EnsemblBacteria" id="CAI49269">
        <property type="protein sequence ID" value="CAI49269"/>
        <property type="gene ID" value="NP_2356A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702949"/>
    <dbReference type="KEGG" id="nph:NP_2356A"/>
    <dbReference type="HOGENOM" id="CLU_975272_0_0_2"/>
    <dbReference type="OrthoDB" id="275229at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
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    </dbReference>
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    </dbReference>
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        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22356">
        <property type="entry name" value="Sau3AI_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.600.10">
        <property type="entry name" value="DNA mismatch repair MutH/Restriction endonuclease, type II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011337">
        <property type="entry name" value="DNA_rep_MutH/RE_typeII_Sau3AI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037057">
        <property type="entry name" value="DNA_rep_MutH/T2_RE_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02976">
        <property type="entry name" value="MutH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair MutH/Type II restriction enzyme Sau3AI" evidence="5">
        <location>
            <begin position="58"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="188"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="195"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI49269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="285" mass="32055" checksum="4C1B117D3AD91C99" modified="2017-05-10" version="1">MFDLPSGPDAEQEDILRATNALLGRSFGEIDEEIQGMADDERARSKHGVANVIESGYFNIETNSSAEPDFGKAGIELKVTPLRLTGNNELVRPKERLVLCMCDYMEVVDADHWTDVPALRKKLEQVLIIWYIHIVGQDRRTYPIVWWTLWEPMTEPEWSEIIQSDFEICKKRILNGEVPSEKHTQLLGTCPKHGGGYDRENPEESPRSSRVAQDAHPTLDYAEKRGWSIGLGGCMDLFESATGLEKASRGRASGIELTALWGAAQERTPHDIPRFSDAINEDDDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW52</accession>
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        <submittedName>
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        </submittedName>
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    </gene>
    <organism evidence="2 3">
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        <lineage>
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            <taxon>Methanobacteriati</taxon>
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            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
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            <taxon>Natronomonas</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A1U7EW52"/>
    <dbReference type="STRING" id="348780.NP_2420A"/>
    <dbReference type="EnsemblBacteria" id="CAI49301">
        <property type="protein sequence ID" value="CAI49301"/>
        <property type="gene ID" value="NP_2420A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701438"/>
    <dbReference type="KEGG" id="nph:NP_2420A"/>
    <dbReference type="eggNOG" id="arCOG00691">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009942_6_1_2"/>
    <dbReference type="OrthoDB" id="8791at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01300">
        <property type="entry name" value="YtcJ_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013108">
        <property type="entry name" value="Amidohydro_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033932">
        <property type="entry name" value="YtcJ-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22642">
        <property type="entry name" value="IMIDAZOLONEPROPIONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22642:SF2">
        <property type="entry name" value="PROTEIN LONG AFTER FAR-RED 3"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase 3" evidence="1">
        <location>
            <begin position="52"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="501" mass="54372" checksum="C9739C69E03F51E6" modified="2017-05-10" version="1">MTRAADRIFENAEVHSLGESDEIHEAVAVRDGRIVRVGSDYEVEFLRGVDTDVVDCDGNVLLPGFIDAHTHLEILGRRLVRADLDTGTRTAALDRLHEAADGDDAWVLGYGYDESDWDDGRLLQRAELDSVSTDRPVVAFREDLHTASANSVVFERYGDELPEAGVERTDGEPTGVVREAAAERLRRETAPDRAETRQLVTAARDDAHERGVTGVHEMVRASEAPAVYRTMARDGELGLRVRLYYWADHLDAVEETGLVADCGSDLVEVGGIKAYADGSLGARTARLSEPYADADGRGEWLTDPERLRELAARVDDLGLQFAVHAIGDEAVGAVLSALPDDSDRRHRIEHAELLPGAAADFDAVASMQPNFLRWARAGGLYAARLGAERAATVDRFADLLAAGVPVAFGSDCMPLDPLYGVEQAVTAPEDSQRLTVTEALRAYTTGAAYAGHDEDRLGTIEVGKRADLVLLEESPWAVDDDAIADIDVTMTVVDGDVVYRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW85</accession>
    <name>A0A1U7EW85_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">YyaL family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2520A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
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            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW85"/>
    <dbReference type="STRING" id="348780.NP_2520A"/>
    <dbReference type="EnsemblBacteria" id="CAI49351">
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        <property type="gene ID" value="NP_2520A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701473"/>
    <dbReference type="KEGG" id="nph:NP_2520A"/>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014051_4_2_2"/>
    <dbReference type="OrthoDB" id="202131at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
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        <property type="entry name" value="Ssp411"/>
    </dbReference>
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        <property type="entry name" value="Ssp411-like_TRX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42899:SF1">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="507"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="538" mass="58582" checksum="BF5C19507B88BD4D" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWA5</accession>
    <name>A0A1U7EWA5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2566A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
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                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWA5"/>
    <dbReference type="STRING" id="348780.NP_2566A"/>
    <dbReference type="EnsemblBacteria" id="CAI49374">
        <property type="protein sequence ID" value="CAI49374"/>
        <property type="gene ID" value="NP_2566A"/>
    </dbReference>
    <dbReference type="GeneID" id="41345089"/>
    <dbReference type="KEGG" id="nph:NP_2566A"/>
    <dbReference type="HOGENOM" id="CLU_2930291_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053463">
        <property type="entry name" value="Brz_Regulator"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23454">
        <property type="entry name" value="Zn_ribbon_Brz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="60" mass="6474" checksum="B549FF782663FC8E" modified="2017-05-10" version="1">MPSATVACPYCETDNTVTVPDGVDIKKVVKSYRTGIRGDKNVSEAECAGGHHFGVKYRSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWC1</accession>
    <name>A0A1U7EWC1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GNAT family acetyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2588A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323010.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWC1"/>
    <dbReference type="STRING" id="348780.NP_2588A"/>
    <dbReference type="EnsemblBacteria" id="CAI49385">
        <property type="protein sequence ID" value="CAI49385"/>
        <property type="gene ID" value="NP_2588A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701499"/>
    <dbReference type="KEGG" id="nph:NP_2588A"/>
    <dbReference type="eggNOG" id="arCOG04563">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2550131_0_0_2"/>
    <dbReference type="OrthoDB" id="333505at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043854">
        <property type="entry name" value="DUF5816"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19133">
        <property type="entry name" value="DUF5816"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="78" mass="8925" checksum="C669BA5646613786" modified="2017-05-10" version="1">MEERTGPDGETLYLSREAERGNKAPFRVVYSDAEGTRRWGFFCTNCDTFDNAVDAMGRIKCNECANFHKAEEWDAAHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWG0</accession>
    <name>A0A1U7EWG0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Fla cluster protein FlaD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">flaD</name>
        <name evidence="5" type="ordered locus">NP_2686A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Archaeal flagellum</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323059.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWG0"/>
    <dbReference type="STRING" id="348780.NP_2686A"/>
    <dbReference type="EnsemblBacteria" id="CAI49434">
        <property type="protein sequence ID" value="CAI49434"/>
        <property type="gene ID" value="NP_2686A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702710"/>
    <dbReference type="KEGG" id="nph:NP_2686A"/>
    <dbReference type="eggNOG" id="arCOG02964">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_951929_0_0_2"/>
    <dbReference type="OrthoDB" id="121879at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097589">
        <property type="term" value="C:archaeal-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097588">
        <property type="term" value="P:archaeal or bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006752">
        <property type="entry name" value="Arch_fla_DE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052494">
        <property type="entry name" value="Flagella_assembly_related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40698:SF2">
        <property type="entry name" value="FLAGELLA-RELATED PROTEIN C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40698">
        <property type="entry name" value="FLAGELLA-RELATED PROTEIN E-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04659">
        <property type="entry name" value="Arch_fla_DE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0974">Archaeal flagellum</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Archaeal flagella protein FlaD/E" evidence="4">
        <location>
            <begin position="255"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="8"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="49"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="75"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="163"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="219"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="347" mass="37648" checksum="E1C4FD1FDB12B3CE" modified="2017-05-10" version="1">MSSQLNPRDYDLGELRDAVRETTRRERRNEQTRDEGLDDADGEGGQPIEPERSQQPRETKTETQGRRSNTGSQPERPERRDRRRGDEPAEYQPASQRRPGQTAAAERDDPETPAEQPQTSVGGAEDAEAESTEALTSSESEAGDAFADASSAQEDAFESPGGDDSPSAATPTASTADTSTAPSATAESEPSPGADAGDDTDSIADDPAAYLQSRHEKRRAAGREPETGPDTERSDPAIAQEDFELLARLSENSDDRPYLQRLPDEYSAQLEIFDWLDTMVAKAGHESTVEALAYYESVGWLSEESRAQLEDFADGLAAADAGGHPLTVADHRESLLYIARLAHRLGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWG7</accession>
    <name>A0A1U7EWG7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2688A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323060.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWG7"/>
    <dbReference type="STRING" id="348780.NP_2688A"/>
    <dbReference type="EnsemblBacteria" id="CAI49435">
        <property type="protein sequence ID" value="CAI49435"/>
        <property type="gene ID" value="NP_2688A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701525"/>
    <dbReference type="KEGG" id="nph:NP_2688A"/>
    <dbReference type="eggNOG" id="arCOG14725">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_808021_0_0_2"/>
    <dbReference type="OrthoDB" id="238756at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="343" mass="38169" checksum="0BA6F4EC85C5359C" modified="2017-05-10" version="1">MATQDARSTTLRDFNVDPNVVAREHTPSLFPRERFVRCTTCGVTVPYSVNAEGPIRRALDELSSIPCGRYELPDVSVGDHVPTVTPEHPTKGRSVVCESCGRSARLHGDREAVLHSLADIPCIDQLSYTELVNLVFSPTSFTPVLPDLGISNWYGRLGSRRSDVVRQFRHDRFQYTVYIKEQTDGTYTAALATSPNIDGDPVGRLRFPSDDPADPAARKTAIRLVTFLSATDPLTAGEFSKLQAAHEEVDRKQRKGWLDDAYGTAREKFSERFNDTPEAFTNAFGDSSADEREVIRRLVGSGGFTDMDEPAFAREILGRDHEFPGFIDFVADHHNWSPPVPEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWI5</accession>
    <name>A0A1U7EWI5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">YgfD family GTPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ygfD</name>
        <name evidence="6" type="ordered locus">NP_2712A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIMIBI class G3E GTPase family. ArgK/MeaB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323072.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWI5"/>
    <dbReference type="STRING" id="348780.NP_2712A"/>
    <dbReference type="EnsemblBacteria" id="CAI49447">
        <property type="protein sequence ID" value="CAI49447"/>
        <property type="gene ID" value="NP_2712A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702523"/>
    <dbReference type="KEGG" id="nph:NP_2712A"/>
    <dbReference type="eggNOG" id="arCOG01226">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043725_1_0_2"/>
    <dbReference type="OrthoDB" id="21324at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03114">
        <property type="entry name" value="MMAA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052040">
        <property type="entry name" value="GTPase/Isobutyryl-CoA_mutase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005129">
        <property type="entry name" value="GTPase_ArgK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00750">
        <property type="entry name" value="lao"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43087:SF1">
        <property type="entry name" value="LAO_AO TRANSPORT SYSTEM ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43087">
        <property type="entry name" value="LYSINE/ARGININE/ORNITHINE TRANSPORT SYSTEM KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03308">
        <property type="entry name" value="MeaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI49447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="353" mass="37906" checksum="9DAAF9B34E1EC482" modified="2017-05-10" version="1">MSDDETLIEELLAGKHRALARVITKIEDRSPGYRDLVSSLHAHTGNAEVIGITGSPGAGKSTLVDKMAATYRERGETVGVIAIDPSSPFTGGAVLGDRIRMASNVGDMDVFFRSMSARGSLGGLSTATTDAVKALDAFGKDKIIVETVGAGQNEIEVVKTADTVAVLVPPGSGDDVQMLKAGILEIADVFVVNKADLDGADRTVMELREMVEMRDNSPMAGHHGADTIDIGGGTDDPDGDADGWEPPIVETVAKSGDGVEEFLGVLADHVEWLKETGERQTQVEKRYAAEIRTLLREDTATLLEAEIEARGGLDQYVQQVLQRETDPYTVADDIVEPLADCVRKRREHEDDYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWI7</accession>
    <name>A0A1U7EWI7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">1,4-dihydroxy-2-naphthoate octaprenyltransferase</fullName>
            <shortName evidence="8">DHNA-octaprenyltransferase</shortName>
            <ecNumber evidence="8">2.5.1.74</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">menA1</name>
        <name evidence="8" type="synonym">menA</name>
        <name evidence="9" type="ordered locus">NP_2734A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Conversion of 1,4-dihydroxy-2-naphthoate (DHNA) to demethylmenaquinone (DMK).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>an all-trans-polyprenyl diphosphate + 1,4-dihydroxy-2-naphthoate + H(+) = a 2-demethylmenaquinol + CO2 + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:26478"/>
            <dbReference type="Rhea" id="RHEA-COMP:9563"/>
            <dbReference type="Rhea" id="RHEA-COMP:9564"/>
            <dbReference type="ChEBI" id="CHEBI:11173"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:55437"/>
            <dbReference type="ChEBI" id="CHEBI:58914"/>
            <dbReference type="EC" id="2.5.1.74"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Quinol/quinone metabolism; menaquinone biosynthesis; menaquinol from 1,4-dihydroxy-2-naphthoate: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the MenA family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.74" evidence="8"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49458.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_148215437.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWI7"/>
    <dbReference type="STRING" id="348780.NP_2734A"/>
    <dbReference type="EnsemblBacteria" id="CAI49458">
        <property type="protein sequence ID" value="CAI49458"/>
        <property type="gene ID" value="NP_2734A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702906"/>
    <dbReference type="KEGG" id="nph:NP_2734A"/>
    <dbReference type="eggNOG" id="arCOG00480">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043611_1_2_2"/>
    <dbReference type="OrthoDB" id="203724at2157"/>
    <dbReference type="UniPathway" id="UPA00079">
        <property type="reaction ID" value="UER00168"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046428">
        <property type="term" value="F:1,4-dihydroxy-2-naphthoate polyprenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009234">
        <property type="term" value="P:menaquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042371">
        <property type="term" value="P:vitamin K biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13962">
        <property type="entry name" value="PT_UbiA_UBIAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01937">
        <property type="entry name" value="MenA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004657">
        <property type="entry name" value="MenA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026046">
        <property type="entry name" value="UBIAD1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00751">
        <property type="entry name" value="menA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004751">
        <property type="entry name" value="PRK06080.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13929">
        <property type="entry name" value="1,4-DIHYDROXY-2-NAPHTHOATE OCTAPRENYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13929:SF0">
        <property type="entry name" value="UBIA PRENYLTRANSFERASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005355">
        <property type="entry name" value="UBIAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 8" id="KW-0474">Menaquinone biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="149"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="235"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="257"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="295"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI49458.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="312" mass="32427" checksum="816D249408F1E44F" modified="2017-05-10" version="1">MTAAASSTKAWLMAARPQTLPAGAAPVIVGTGLAVYHGVFAPLPALFALFGALLLQIGTNFANDYYDAVRGADTEDRDGFTRVTAGGLIEPAAVKRAMYATFGLSMVIGLYLVAVGGVPILVVGLASIVAGITYSGGPLPYGYRGLGDLFVFVFFGLVAVAGTYYVQAAAHGGWFPLWIPEGTLPAAAVVAGLPAAGFSTAILVVNNIRDRETDAEAGKHTLAVILGYRLSRIEWSLMAGLGYAIPVVFWLDGYPATVLLPLVTAPLAIAIGRTVWNRTDGEALNPALQRTGKLLFAHSVCFALGFAAPAVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWJ0</accession>
    <name>A0A1U7EWJ0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="17">3-hydroxyacyl-CoA dehydrogenase / enoyl-CoA hydratase</fullName>
            <ecNumber evidence="17">1.1.1.35</ecNumber>
            <ecNumber evidence="17">4.2.1.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">hbd2</name>
        <name evidence="17" type="synonym">fadA2</name>
        <name evidence="17" type="ordered locus">NP_2754A</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.35" evidence="17"/>
    <dbReference type="EC" id="4.2.1.17" evidence="17"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323093.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWJ0"/>
    <dbReference type="STRING" id="348780.NP_2754A"/>
    <dbReference type="EnsemblBacteria" id="CAI49468">
        <property type="protein sequence ID" value="CAI49468"/>
        <property type="gene ID" value="NP_2754A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702793"/>
    <dbReference type="KEGG" id="nph:NP_2754A"/>
    <dbReference type="eggNOG" id="arCOG00249">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010448_2_1_2"/>
    <dbReference type="OrthoDB" id="39812at2157"/>
    <dbReference type="UniPathway" id="UPA00659"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003857">
        <property type="term" value="F:(3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000009">
        <property type="entry name" value="Fatty oxidation complex, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.12.10:FF:000001">
        <property type="entry name" value="Probable enoyl-CoA hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006176">
        <property type="entry name" value="3-OHacyl-CoA_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006108">
        <property type="entry name" value="3HC_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23309">
        <property type="entry name" value="3-HYDROXYACYL-COA DEHYROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23309:SF49">
        <property type="entry name" value="PEROXISOMAL BIFUNCTIONAL ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00725">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02737">
        <property type="entry name" value="3HCDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 17" id="KW-0456">Lyase</keyword>
    <keyword evidence="13" id="KW-0511">Multifunctional enzyme</keyword>
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    <keyword evidence="10" id="KW-0576">Peroxisome</keyword>
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        <location>
            <begin position="8"/>
            <end position="187"/>
        </location>
    </feature>
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        <location>
            <begin position="190"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase C-terminal" evidence="15">
        <location>
            <begin position="300"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI49468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="651" mass="71050" checksum="B07647C8A19DE4C5" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWJ2</accession>
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        </submittedName>
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    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
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            <taxon>Stenosarchaea group</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="121" mass="11922" checksum="282984B693E1BD18" modified="2017-05-10" version="1">MSDTGSLRAEVAAAFDVGTWLFSTVAALVGFVVGLEAVGSTPGAVAAAFLAAAAATLLGRLDAPTPASLDDRLAGKTAVLVVAVVAGGLAIARPLAGIVVAVACYLTVWLRSFAPTAAPDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWK8</accession>
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        </submittedName>
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    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
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        <lineage>
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            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="CAI49479">
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        <property type="gene ID" value="NP_2776A"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="240954at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
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            <end position="48"/>
        </location>
    </feature>
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            <end position="73"/>
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        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="119" mass="11865" checksum="4A0E1F574BD87F3E" modified="2017-05-10" version="1">MSFAGHLHAIRYGSRSELAVAAAVVVGIGATAGHWLGLVVAGGLLGVVASSTERALVLGCYFGFFVLVAYGVVLSWYGVLGAVAGAFPLSVGLVAVSVLVPTATAAIVRYGTPVTTTDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWL3</accession>
    <name>A0A1U7EWL3_NATPD</name>
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        <submittedName>
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        </submittedName>
    </protein>
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    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S45 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
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    </dbReference>
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    <dbReference type="STRING" id="348780.NP_2778A"/>
    <dbReference type="EnsemblBacteria" id="CAI49480">
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    </dbReference>
    <dbReference type="GeneID" id="3701554"/>
    <dbReference type="KEGG" id="nph:NP_2778A"/>
    <dbReference type="eggNOG" id="arCOG04082">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011790_0_1_2"/>
    <dbReference type="OrthoDB" id="56056at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017000">
        <property type="term" value="P:antibiotic biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.439.10">
        <property type="entry name" value="Penicillin Amidohydrolase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014395">
        <property type="entry name" value="Pen/GL7ACA/AHL_acylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043147">
        <property type="entry name" value="Penicillin_amidase_A-knob"/>
    </dbReference>
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        <property type="entry name" value="Penicillin_amidase_dom1"/>
    </dbReference>
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        <property type="entry name" value="Penicillin_amidase_N_B-knob"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002692">
        <property type="entry name" value="S45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34218:SF4">
        <property type="entry name" value="ACYL-HOMOSERINE LACTONE ACYLASE QUIP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01804">
        <property type="entry name" value="Penicil_amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0865">Zymogen</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="230"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="230"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="813" mass="89333" checksum="804CF4028BD52E6C" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWP7</accession>
    <name>A0A1U7EWP7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DRTGG domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2856A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323144.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWP7"/>
    <dbReference type="STRING" id="348780.NP_2856A"/>
    <dbReference type="EnsemblBacteria" id="CAI49519">
        <property type="protein sequence ID" value="CAI49519"/>
        <property type="gene ID" value="NP_2856A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701574"/>
    <dbReference type="KEGG" id="nph:NP_2856A"/>
    <dbReference type="eggNOG" id="arCOG00099">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040984_0_0_2"/>
    <dbReference type="OrthoDB" id="50320at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd03109">
        <property type="entry name" value="DTBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1390.20">
        <property type="entry name" value="HprK N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010766">
        <property type="entry name" value="DRTGG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050500">
        <property type="entry name" value="Phos_Acetyltrans/Butyryltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028979">
        <property type="entry name" value="Ser_kin/Pase_Hpr-like_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43356">
        <property type="entry name" value="PHOSPHATE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43356:SF2">
        <property type="entry name" value="PHOSPHATE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13500">
        <property type="entry name" value="AAA_26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07085">
        <property type="entry name" value="DRTGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75138">
        <property type="entry name" value="HprK N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DRTGG" evidence="2">
        <location>
            <begin position="214"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="87"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="352" mass="37685" checksum="A483205D8E63FEA9" modified="2017-05-10" version="1">MTILVTSLEPSTGKTAVTLAVAETLRERDETVGYMKPKGTRLESAVGKTLDEDPKLARELLGLDAELHELEPIVYSPTFVTEVVRGREDEEALREQLETQFESLAEETDRMLLEGGDTLSTGRSVNLSDIDIAELLDATVVLVASYTEPKDVDELLDAADRLGDRLGGILFNDVPDAAFDSLATDVVPFLESRGVRVYGTVPRDETLSGSSVSEIAAELGADVLTNNVPTDGLVQRFVVGATSAGEVLSELRRVRDAALVTGGDRTDVQTAALEASGVECLVLTGGFKPSSAVVGRAESADVPVLAVRTDTKTTLDRLEALVRSGRTRDAETVDRMQSLLADYADIDAMTEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWR8</accession>
    <name>A0A1U7EWR8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Peroxiredoxin</fullName>
            <ecNumber evidence="5">1.11.1.15</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_2900A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.11.1.15" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323166.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWR8"/>
    <dbReference type="STRING" id="348780.NP_2900A"/>
    <dbReference type="EnsemblBacteria" id="CAI49541">
        <property type="protein sequence ID" value="CAI49541"/>
        <property type="gene ID" value="NP_2900A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701589"/>
    <dbReference type="KEGG" id="nph:NP_2900A"/>
    <dbReference type="eggNOG" id="arCOG00310">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_14_2_2"/>
    <dbReference type="OrthoDB" id="6924at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050455">
        <property type="entry name" value="Tpx_Peroxidase_subfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43110">
        <property type="entry name" value="THIOL PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43110:SF1">
        <property type="entry name" value="THIOL PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="2" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="4">
        <location>
            <begin position="2"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity" evidence="3">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="159" mass="16951" checksum="8B53AE82CEA7F73E" modified="2017-05-10" version="1">MVAEGDDAPDFTAPVRTSDGNISEFTLSEALADGPVVLAFFPGAFTSVCTGEMTTLRDRLDDIEAGGASLYGVSIDSPFSLGEFAEQNDLEFPLIGDTNKEVIDAYDVVMDFEDLGIEGVAKRSVFVVDEDGTVTYSWVSDDPGVEPDYDELEAAANDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWV8</accession>
    <name>A0A1U7EWV8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ba3-type terminal oxidase subunit CbaE</fullName>
            <ecNumber evidence="3">1.9.3.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cbaE</name>
        <name evidence="3" type="ordered locus">NP_2968A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.9.3.-" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323199.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWV8"/>
    <dbReference type="STRING" id="348780.NP_2968A"/>
    <dbReference type="EnsemblBacteria" id="CAI49575">
        <property type="protein sequence ID" value="CAI49575"/>
        <property type="gene ID" value="NP_2968A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702565"/>
    <dbReference type="KEGG" id="nph:NP_2968A"/>
    <dbReference type="eggNOG" id="arCOG10155">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_200163_0_0_2"/>
    <dbReference type="OrthoDB" id="170780at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058444">
        <property type="entry name" value="DUF8131"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26452">
        <property type="entry name" value="DUF8131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="DUF8131" evidence="2">
        <location>
            <begin position="5"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="67" mass="6638" checksum="CDC0E18130300ED4" modified="2017-05-10" version="1">MPIDALSPRQAAAVGALALIPVALYGYTLSATAAAFSTVNVLLTLAVLYIAMSPVEGGHHGDSGAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWZ7</accession>
    <name>A0A1U7EWZ7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UspA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3098A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323262.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWZ7"/>
    <dbReference type="STRING" id="348780.NP_3098A"/>
    <dbReference type="EnsemblBacteria" id="CAI49640">
        <property type="protein sequence ID" value="CAI49640"/>
        <property type="gene ID" value="NP_3098A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701651"/>
    <dbReference type="KEGG" id="nph:NP_3098A"/>
    <dbReference type="eggNOG" id="arCOG02053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_19_1_2"/>
    <dbReference type="OrthoDB" id="307404at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF6">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN UP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="3"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="156" mass="16463" checksum="A1C9E9781DCD87B6" modified="2017-05-10" version="1">MAIIAAVDSSDRAIAVVERASELSREYGVALHVVHVAEPTVDFREELIDVSADALNPEGGEVPKQRLDDLRTAAAETAAAAAADVDDLEAYEAVGLVGNPATAIKQYASEHDAEYIVVSGRKRHPLGQALFGSVTQALLLNANRPVVAVPHDRADS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX18</accession>
    <name>A0A1U7EX18_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Urease accessory protein UreH-like transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3138A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323282.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX18"/>
    <dbReference type="STRING" id="348780.NP_3138A"/>
    <dbReference type="EnsemblBacteria" id="CAI49660">
        <property type="protein sequence ID" value="CAI49660"/>
        <property type="gene ID" value="NP_3138A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701665"/>
    <dbReference type="KEGG" id="nph:NP_3138A"/>
    <dbReference type="eggNOG" id="arCOG04638">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032635_1_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039447">
        <property type="entry name" value="UreH-like_TM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42208:SF1">
        <property type="entry name" value="HEAVY METAL TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42208">
        <property type="entry name" value="HEAVY METAL TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13386">
        <property type="entry name" value="DsbD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="234"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Urease accessory protein UreH-like transmembrane" evidence="2">
        <location>
            <begin position="26"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="269" mass="27769" checksum="ACBD064E6D938004" modified="2017-05-10" version="1">MLSVQTCNDPSVSAVPGTETLSLGVFALVGLLAGAHCLGMCGPLVTVYSDRLGNDGRRDVLTLRQVRQHALFNLGRAVSYGLIGGIAGLAGSLVFVEAGHLTTAATEIHATMGLVVGLAIVGIGIAYIRHGGAGLPHLTIPLLGGQFSRLQQGMVARVDRWVAGPRIAGLGAVHGLFPCPIIYPAYLYAFVQASPAGGAAALVVLGLGTVPSLFLYGTLLGGLSATTRQRLHRLLGVAFVVLGYIPLQHGLAAMGVPLPHPPIPYYSPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX37</accession>
    <name>A0A1U7EX37_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Electron transfer flavoprotein alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">etfA</name>
        <name evidence="5" type="ordered locus">NP_3168A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ETF alpha-subunit/FixB family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323297.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX37"/>
    <dbReference type="STRING" id="348780.NP_3168A"/>
    <dbReference type="EnsemblBacteria" id="CAI49675">
        <property type="protein sequence ID" value="CAI49675"/>
        <property type="gene ID" value="NP_3168A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702678"/>
    <dbReference type="KEGG" id="nph:NP_3168A"/>
    <dbReference type="eggNOG" id="arCOG00447">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034178_0_1_2"/>
    <dbReference type="OrthoDB" id="307696at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01715">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1220:FF:000001">
        <property type="entry name" value="Electron transfer flavoprotein, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001308">
        <property type="entry name" value="ETF_a/FixB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033947">
        <property type="entry name" value="ETF_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014731">
        <property type="entry name" value="ETF_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153:SF1">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00766">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000089">
        <property type="entry name" value="Electra_flavoP_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Electron transfer flavoprotein alpha/beta-subunit N-terminal" evidence="4">
        <location>
            <begin position="3"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="322" mass="34142" checksum="14E069EBF20BF6C3" modified="2017-05-10" version="1">MTVLAITEHRRGELRDVSLELVSAGRELADELGGDLALAVIGGDVDAFADELNRDGVDTIYTVDEGEEFNHGVYTQAIEQLHDELDPTALLMPNSVNGLDYAPAVAQSLDIPLVTDVIGLEGDETVEVTREQYGGKVETTYDIDADQYALTVRPAEWPTAEADGDAAIEAFDADIDEDALGTTVNGFEEVGGGDVDISEADVLVSVGRGIEEEENLPLVEELADALDATLSSSRPIVDNGWLPANRQVGQSGKVVTPDVYIAIGISGAVQHVAGMKGSDTIVAINTDPNAPIYDIADYGIVDDLFEVVPELIEEFGGEAPDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX80</accession>
    <name>A0A1U7EX80_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CRISPR-associated endoribonuclease Cas6</fullName>
            <ecNumber evidence="3">3.1.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cas6</name>
        <name evidence="3" type="ordered locus">NP_3262A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.-.-" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323343.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX80"/>
    <dbReference type="STRING" id="348780.NP_3262A"/>
    <dbReference type="EnsemblBacteria" id="CAI49722">
        <property type="protein sequence ID" value="CAI49722"/>
        <property type="gene ID" value="NP_3262A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701706"/>
    <dbReference type="KEGG" id="nph:NP_3262A"/>
    <dbReference type="eggNOG" id="arCOG05135">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_975260_0_0_2"/>
    <dbReference type="OrthoDB" id="116088at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21141">
        <property type="entry name" value="Cas6_III-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019267">
        <property type="entry name" value="CRISPR-assoc_Cas6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045747">
        <property type="entry name" value="CRISPR-assoc_prot_Cas6_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10040">
        <property type="entry name" value="CRISPR_Cas6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRISPR-associated protein Cas6 C-terminal" evidence="2">
        <location>
            <begin position="155"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="290" mass="31418" checksum="B4679E4C37A22CE3" modified="2017-05-10" version="1">MASAGEKGTVVPQVRQVTVSLTPRTRFPVPKSDGYSVYSALLSVLSDVSEEIGSSVHDSPLGSLHNSGLLGPRGSSDRPHHKLLLPDETYELSLGIVHPEDGEVFQALVNALVLQNETVELSHGELEVREFESSNATHEELLAEASQYDDPTVEMTFQTATCIEEADGVTTMFPTRGAVFNSLLGKWNRSVPEELELDLTREEVDASVIEKPNDRSYQTHSVLVNRVKNDNGENRNIFRQGFTGECAYAFKGASESVENAVTALALFGEYSGVGSAVARGCGQVSTEVKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXH6</accession>
    <name>A0A1U7EXH6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Compatible solute transport protein (Probable substrate choline/glycine betaine)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">opuD</name>
        <name evidence="8" type="synonym">tp58</name>
        <name evidence="8" type="ordered locus">NP_3588A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
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    </organism>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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        <property type="protein sequence ID" value="CAI49885.1"/>
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    <dbReference type="EnsemblBacteria" id="CAI49885">
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        <property type="gene ID" value="NP_3588A"/>
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    <dbReference type="KEGG" id="nph:NP_3588A"/>
    <dbReference type="eggNOG" id="arCOG04540">
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    <dbReference type="OrthoDB" id="141573at2157"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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        <property type="entry name" value="BCCT"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-0813">Transport</keyword>
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        </location>
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        <location>
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            <begin position="486"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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        <source>
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        </source>
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            <dbReference type="EMBL" id="CAI49885.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
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    <sequence length="527" mass="55125" checksum="7639C99D512169D3" modified="2017-05-10" version="1"> </sequence>
</entry>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
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                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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            <end position="350"/>
        </location>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="350" mass="39308" checksum="9C09919D3C155DCB" modified="2017-05-10" version="1">MLYDAVAGLDLNVDEYSLERRERDTSSGFTRMTTIVSLRGDGCVGRGEDVTYDTDAHLSLRDSGTAFDITGEYTLDSFSEALSGIDVFHGETPGQTVFRNYRRWAFESAALDLALKQADTNLADRLDRSYDPVRFVVSTRLEEPPTGDRVLEWLDRDSDLEFKLDPTSEWPAEAIERLAATDAVRILDLKGQYHGTTVDQPADPELYERVIESFPEALIEDPELNEETEPLFEGHEPRVTWDYPIRGVDTVKELPWEPEWLNIKPSRFGSVRSLFDTLDYCREHGIQMFGGGQFELDVGREHLHAIASLFYPDAPNDVAPKAYNDPNPSGGLPSSPLTPPTAPSGLSWDR</sequence>
</entry>
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    <accession>A0A1U7EXK7</accession>
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        <lineage>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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        <property type="gene ID" value="NP_3672A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702918"/>
    <dbReference type="KEGG" id="nph:NP_3672A"/>
    <dbReference type="eggNOG" id="arCOG01877">
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <feature type="domain" description="Cobalamin-independent methionine synthase MetE N-terminal" evidence="1">
        <location>
            <begin position="87"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="327" mass="35706" checksum="44D33DCBDD43A383" modified="2017-05-10" version="1">MTEVLATTPGLYPLPDWAKDELSDLKGHQKDDLISGDESDAIVDAYERARADVLEWQTDAGIDRVVEGQLRWDDMLAHPLCVHDSVETRGIVRYYDNNNFYREPVVQDALTPDGDVADEIAAADGVNQAVLPGPYSLSELATDEHYGDDAAFLDAVADFLAAEAGALPDVETLFLLEPSLVTDAPDDGADERASEAIDAVADAVDAEVVVHTYWGSIPEKPYAHLMDADIDAIGFDFVTDHEGSVYNIQEYGTKDSIALGVVDGQNTLVETPEEIAERVEWVEEQVPVSEFEQLYVTSNTELFYLPTNKAEAKLNALATAAAEEVPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXM5</accession>
    <name>A0A1U7EXM5_NATPD</name>
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        <submittedName>
            <fullName evidence="2">DUF368 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3722A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXM5"/>
    <dbReference type="STRING" id="348780.NP_3722A"/>
    <dbReference type="EnsemblBacteria" id="CAI49952">
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        <property type="gene ID" value="NP_3722A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701847"/>
    <dbReference type="KEGG" id="nph:NP_3722A"/>
    <dbReference type="eggNOG" id="arCOG04565">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055621_0_0_2"/>
    <dbReference type="OrthoDB" id="313161at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007163">
        <property type="entry name" value="VCA0040-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37308">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37308:SF1">
        <property type="entry name" value="POLYPRENYL-PHOSPHATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04018">
        <property type="entry name" value="VCA0040-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="256"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="298"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="325" mass="33451" checksum="2CA3C5D4CC68F0C8" modified="2017-05-10" version="1">MSATPTGARAWLAVYLKGVCMGAADTVPGVSGGTIAVVLGIYERLIAALTSLDPKAATTLREIHTANGRARLAAELVRMDVPFLLVLGAGVLSAAATVATAMNVAVTQYPAPTYAFFFGLIAASAVVLYRYVDAGTPGRLLAAGVGFVFAFVVTDPSLAGDMPATAPMLFVAGAIAVSAMVLPGVSGAFLLLVLGQYEYVSGIPRRFVEGLLSGGGETFIEAAVAFGVFLGGALVGLFTVAYAVRAALKRYRQATLVFLVSLMVGALRLPVHEMAVGVETRPFDMAVLGIDTVPTPRSGAIVAVAAALGVAAVMVLDRYTEDLQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXN9</accession>
    <name>A0A1U7EXN9_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">NP_3700A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323559.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXN9"/>
    <dbReference type="STRING" id="348780.NP_3700A"/>
    <dbReference type="EnsemblBacteria" id="CAI49941">
        <property type="protein sequence ID" value="CAI49941"/>
        <property type="gene ID" value="NP_3700A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701842"/>
    <dbReference type="KEGG" id="nph:NP_3700A"/>
    <dbReference type="eggNOG" id="arCOG06218">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095957_0_0_2"/>
    <dbReference type="OrthoDB" id="170690at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022170">
        <property type="entry name" value="MUL1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12483">
        <property type="entry name" value="GIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="241"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="E3 Ubiquitin ligase MUL1-like" evidence="13">
        <location>
            <begin position="84"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF12483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAI49941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="260" mass="28004" checksum="5038A78E6A0952F8" modified="2017-05-10" version="1">MSAVGMVLGLLFAGGGVWLGWSGWQRRQERQTIAARETTDALSVTPGPTEVSGTAVTDDDRTLTAPFTDASCLLAEWQIEEWDTSGDNSSWQTLDTGTVAVPFAVDDGTGTVRVAPEGASIELTDPWRTETVEIDDEPPATVKQFLERDSTPGEATGSLLPSFGWGNEAGDRRYHQRLLRPGEEVYVHGTATRTDSRSFGDVAHEIRETADDGHRDAELFIVADGDEADVIASRRDAETRLLAGAITVALGATFFVASLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXP9</accession>
    <name>A0A1U7EXP9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3728A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49955.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939627.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXP9"/>
    <dbReference type="STRING" id="348780.NP_3728A"/>
    <dbReference type="EnsemblBacteria" id="CAI49955">
        <property type="protein sequence ID" value="CAI49955"/>
        <property type="gene ID" value="NP_3728A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701849"/>
    <dbReference type="KEGG" id="nph:NP_3728A"/>
    <dbReference type="eggNOG" id="arCOG08152">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_865003_0_0_2"/>
    <dbReference type="OrthoDB" id="383020at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="33"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49955.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="308" mass="33124" checksum="6BAB26D0646E3CC9" modified="2017-05-10" version="1">MRRRVLLSLVSGTAAALAGCADGMEEANDDDGGTPAATPAADDAAAIPEPQMTVEVPDKRQRGEPFSVRLTAENPADEPQAVSRQVSDVTADVTVGEFSSTLPPGETVVWESPPIVYQTIPDDGVVELDVGGDTVSVRIFEPLSVGASHTYEDGVTIHVEGIQTVRAYPHRIDDRRRWRHAGDGAQYVFVFVSATGVHRDGQVGTPPSKRGLLLRHGGQVFRPQTPRYTPLTGVPVPSEYTIDSWGATPRADVSAYDSVDIRRSALDGEHPDENLYPDAAAAPDETLWLFYEVSTEIPATGLRVLASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXV4</accession>
    <name>A0A1U7EXV4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ABC-type transport system periplasmic substrate-binding protein (Probable substrate copper)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">nosD2</name>
        <name evidence="4" type="synonym">abc12s</name>
        <name evidence="4" type="ordered locus">NP_3922A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXV4"/>
    <dbReference type="STRING" id="348780.NP_3922A"/>
    <dbReference type="EnsemblBacteria" id="CAI50052">
        <property type="protein sequence ID" value="CAI50052"/>
        <property type="gene ID" value="NP_3922A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3922A"/>
    <dbReference type="eggNOG" id="arCOG02519">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_426783_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008719">
        <property type="entry name" value="N2O_reductase_NosL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007742">
        <property type="entry name" value="NosD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051550">
        <property type="entry name" value="SCF-Subunits/Alg-Epimerases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22990">
        <property type="entry name" value="F-BOX ONLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22990:SF15">
        <property type="entry name" value="F-BOX ONLY PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05048">
        <property type="entry name" value="NosD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05573">
        <property type="entry name" value="NosL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160387">
        <property type="entry name" value="NosL/MerB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Periplasmic copper-binding protein NosD beta helix" evidence="3">
        <location>
            <begin position="408"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="302"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="666" mass="70197" checksum="82350BFFF949C50C" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXX2</accession>
    <name>A0A1U7EXX2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Thioredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">trxA7</name>
        <name evidence="3" type="ordered locus">NP_3914A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323665.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXX2"/>
    <dbReference type="STRING" id="348780.NP_3914A"/>
    <dbReference type="EnsemblBacteria" id="CAI50048">
        <property type="protein sequence ID" value="CAI50048"/>
        <property type="gene ID" value="NP_3914A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703429"/>
    <dbReference type="KEGG" id="nph:NP_3914A"/>
    <dbReference type="eggNOG" id="arCOG06181">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090892_1_0_2"/>
    <dbReference type="OrthoDB" id="115386at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02966">
        <property type="entry name" value="TlpA_like_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013740">
        <property type="entry name" value="Redoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050553">
        <property type="entry name" value="Thioredoxin_ResA/DsbE_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42852:SF13">
        <property type="entry name" value="PROTEIN DIPZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42852">
        <property type="entry name" value="THIOL:DISULFIDE INTERCHANGE PROTEIN DSBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08534">
        <property type="entry name" value="Redoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="27"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="188" mass="19301" checksum="31A6A77EFD253F43" modified="2017-05-10" version="1">MQRRTVLGGLAGAATLAVGGWAARRGPTAGDSVGNAAGDEDGDGSGGVPAQIETIDAPGSEAGSLVLEEEYPLVVTFFATWCSSCSREMSDLTAAHAEVGDQVPFLSLTGEVVGRSVSRAEVADWWREHDGAWSLGLDPDSQVMNQFSVIGIPSVVLVDADGEVVWSDSGHVDTDALVAEAATVADNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXY2</accession>
    <name>A0A1U7EXY2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3968A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323691.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXY2"/>
    <dbReference type="EnsemblBacteria" id="CAI50075">
        <property type="protein sequence ID" value="CAI50075"/>
        <property type="gene ID" value="NP_3968A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701926"/>
    <dbReference type="KEGG" id="nph:NP_3968A"/>
    <dbReference type="eggNOG" id="arCOG06417">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_982132_0_0_2"/>
    <dbReference type="OrthoDB" id="235231at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="259" mass="28132" checksum="5B4C38BA61731C80" modified="2017-05-10" version="1">MELSRRDALRAGGGLLAAAALSGCVEERVTRRETRVTDNSAWSLSTATEDVALSATEFDNYTERMADRYGDSGVWGSDGDPGEEFETAYVQRYAIKRETPGSPTESEFDLVPDNVDPEAPMLIADSCVALYRTDDGRHRYWLWAATDSTDSRLVRDVNVTEIAAGVRFRDAPLDDAAVPSALGDEATASLGTPPSASFPVRGGALDVTNVRDDTSVYVVEWGGELDGIQSVNGVCEVSHPGAYEFEWTMSLGYNFYETV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY96</accession>
    <name>A0A1U7EY96_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Beta-lactamase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4206A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323810.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY96"/>
    <dbReference type="STRING" id="348780.NP_4206A"/>
    <dbReference type="EnsemblBacteria" id="CAI50194">
        <property type="protein sequence ID" value="CAI50194"/>
        <property type="gene ID" value="NP_4206A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702004"/>
    <dbReference type="KEGG" id="nph:NP_4206A"/>
    <dbReference type="eggNOG" id="arCOG04567">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080903_0_0_2"/>
    <dbReference type="OrthoDB" id="169463at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="222" mass="23874" checksum="A9BEF5211D08F4D9" modified="2017-05-10" version="1">MPMKGSGEGELTVIDEFDGGVGWIAYPDETMRRASHAVESDGELWVLDPVDVDGLDDLLAEYAPVGGVAVLLDRHKRDAAAIAERHGVSVFVPEWMGGVVDDIDAPTERFSDELAGFSVRRLVDNPFWQEAALFDGETLVVPEALGTVDYFRTASEDLGVHPMLRPVPPKCLKEYDPERLLVGHGEGVLDAPAAAIQAAVDGARKGTLPLYAKNLAGLVGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYE7</accession>
    <name>A0A1U7EYE7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">IS1341-type transposase ISNph14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">NP_4358A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323886.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYE7"/>
    <dbReference type="STRING" id="348780.NP_4358A"/>
    <dbReference type="EnsemblBacteria" id="CAI50270">
        <property type="protein sequence ID" value="CAI50270"/>
        <property type="gene ID" value="NP_4358A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702040"/>
    <dbReference type="KEGG" id="nph:NP_4358A"/>
    <dbReference type="eggNOG" id="arCOG00684">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_0_1_2"/>
    <dbReference type="OrthoDB" id="33505at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021027">
        <property type="entry name" value="Transposase_put_HTH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12323">
        <property type="entry name" value="HTH_OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0815">Transposition</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Transposase putative helix-turn-helix" evidence="10">
        <location>
            <begin position="5"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="8">
        <location>
            <begin position="195"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="9">
        <location>
            <begin position="307"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI50270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="422" mass="48483" checksum="F621A56AEB9402E2" modified="2017-05-10" version="1">MKYSPRYRLFPTEEQRQAMDWQRDTVRQLYNHALREFNKIPEDAGTLRQRVWMVRDSIPELKDWWPDLKQVYSTVLQKAVERIRDNINSLGELKEQGYDVGALNWKAPREFRSFTYRQSGFELNKKSGPDGHALLQLKKLKGESREIPVRLHRDLPDHNGIKEVALKKDATGEWYASFTIDTDTPEKPEPGNIDAEDTVGLDLGVCNFIHDSDGRCVDRLNLSGDRERLEREQRSLSRKQKGSNNWEKQRQRVAEVHARMSNKKQDFKHKLAHFYTTEYDAVFVEDLDVKRMLESQQNARNKAEVGWRDFLTILEHHGDKNGCHVVEVEARGTTKECAECGVETAKPLWVREHSCPSCGFELDRDWNAALNVQQRGWNRLGVVHSEATPAETATAVDTLAVSASRVVETGSPCLKEAAQAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYF7</accession>
    <name>A0A1U7EYF7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">HTH domain protein / biotin--[acetyl-CoA-carboxylase] ligase</fullName>
            <ecNumber evidence="5">6.3.4.15</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">birA</name>
        <name evidence="5" type="ordered locus">NP_4378A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.3.4.15" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323896.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYF7"/>
    <dbReference type="STRING" id="348780.NP_4378A"/>
    <dbReference type="EnsemblBacteria" id="CAI50280">
        <property type="protein sequence ID" value="CAI50280"/>
        <property type="gene ID" value="NP_4378A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702554"/>
    <dbReference type="KEGG" id="nph:NP_4378A"/>
    <dbReference type="eggNOG" id="arCOG01940">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051096_0_0_2"/>
    <dbReference type="OrthoDB" id="46252at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004077">
        <property type="term" value="F:biotin--[biotin carboxyl-carrier protein] ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16442">
        <property type="entry name" value="BPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00978">
        <property type="entry name" value="Bifunct_BirA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030855">
        <property type="entry name" value="Bifunct_BirA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004408">
        <property type="entry name" value="Biotin_CoA_COase_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003142">
        <property type="entry name" value="BPL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013196">
        <property type="entry name" value="HTH_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008988">
        <property type="entry name" value="Transcriptional_repressor_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00121">
        <property type="entry name" value="birA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835">
        <property type="entry name" value="BIOTIN PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835:SF5">
        <property type="entry name" value="BIOTIN--PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02237">
        <property type="entry name" value="BPL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03099">
        <property type="entry name" value="BPL_LplA_LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08279">
        <property type="entry name" value="HTH_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50037">
        <property type="entry name" value="C-terminal domain of transcriptional repressors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="4">
        <location>
            <begin position="62"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI50280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="306" mass="32760" checksum="993E8258126495AB" modified="2017-05-10" version="1">MQQTRRQVLDALADGPVAGPELATRLDISRAAVWKHVEALREDGFDIESRNNGYVLVDVPEFGGAAVEHQLDASVDIEYHDRIASTNDRARELAADGAEGVCVLADEQTGARGRLDRPWESPAGGVWLSLVYRPDLPPSQVPVYTLAAAVAATAALREVGVSAEIKWPNDILVDGEKLVGILTEMEGEADRVSWVVVGIGINANIDAASVPDHATTVKEQVGDIDRGALTASIIDRLEALRNAPEDVPEAWRELSTTIGQIVRVETPGETVTGEAVGIEFPGTLVVETDDGKRRIAAGDCEHLRPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYG3</accession>
    <name>A0A1U7EYG3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transport protein (Probable substrate cationic amino acids)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cat5</name>
        <name evidence="8" type="synonym">tp05</name>
        <name evidence="8" type="ordered locus">NP_4372A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323893.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYG3"/>
    <dbReference type="STRING" id="348780.NP_4372A"/>
    <dbReference type="EnsemblBacteria" id="CAI50277">
        <property type="protein sequence ID" value="CAI50277"/>
        <property type="gene ID" value="NP_4372A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703282"/>
    <dbReference type="KEGG" id="nph:NP_4372A"/>
    <dbReference type="eggNOG" id="arCOG00009">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_15_8_2"/>
    <dbReference type="OrthoDB" id="200469at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050367">
        <property type="entry name" value="APC_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770">
        <property type="entry name" value="AMINO ACID TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770:SF7">
        <property type="entry name" value="MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="9"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="84"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="181"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="218"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="274"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="330"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="354"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="385"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="411"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="6">
        <location>
            <begin position="14"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="UspA" evidence="7">
        <location>
            <begin position="478"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI50277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="753" mass="81184" checksum="D756000A8D9015EE" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYL8</accession>
    <name>A0A1U7EYL8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4458A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323935.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYL8"/>
    <dbReference type="STRING" id="348780.NP_4458A"/>
    <dbReference type="EnsemblBacteria" id="CAI50320">
        <property type="protein sequence ID" value="CAI50320"/>
        <property type="gene ID" value="NP_4458A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702068"/>
    <dbReference type="KEGG" id="nph:NP_4458A"/>
    <dbReference type="eggNOG" id="arCOG09132">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_712931_0_0_2"/>
    <dbReference type="OrthoDB" id="236836at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="387" mass="39285" checksum="D5AFF8F270BDB7D3" modified="2017-05-10" version="1">MVDDTRRRAEDSPPDNRKLQPTRRTVLGATGAAAVAALAGCSGVLGEDPPAYADDMLAADGDGVFAVHLDAEWLRTDDGDRPYAEELPDAVDPSLGVDGTVAEVDPLIGYPIGGLLVASLTVSFGAAPYGFGDELLAPFGDDVPDTEAEPEDGSGDDIADGDEANGGADGDADAPADEDDGGTDIDESVRIDSILVADGVGVFRGAFDARAIVAASDGFELADERGAFDVYEGTDDGLFGTEGLAFAVADDVVVVSLTDEGDIDAALDIVAGDESGLTGDDDAAWALATAGEGHITLGAWGIDPDEAAAETAIEGGESLDAATGLVSSLTLGPEEGSATLAAVYPEGDAPERAAHENDIGTSASTRDIDIDGDRLTVDGRWRVPADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYQ2</accession>
    <name>A0A1U7EYQ2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycine cleavage system protein P alpha subunit</fullName>
            <ecNumber evidence="3">1.4.4.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">gcvP1</name>
        <name evidence="3" type="ordered locus">NP_4596A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.4.4.2" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324004.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYQ2"/>
    <dbReference type="STRING" id="348780.NP_4596A"/>
    <dbReference type="EnsemblBacteria" id="CAI50389">
        <property type="protein sequence ID" value="CAI50389"/>
        <property type="gene ID" value="NP_4596A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702744"/>
    <dbReference type="KEGG" id="nph:NP_4596A"/>
    <dbReference type="eggNOG" id="arCOG00077">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004620_0_2_2"/>
    <dbReference type="OrthoDB" id="17655at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004375">
        <property type="term" value="F:glycine dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009116">
        <property type="term" value="P:nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023010">
        <property type="entry name" value="GcvPA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049315">
        <property type="entry name" value="GDC-P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001696">
        <property type="entry name" value="PRK00451.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42806">
        <property type="entry name" value="GLYCINE CLEAVAGE SYSTEM P-PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42806:SF1">
        <property type="entry name" value="GLYCINE DEHYDROGENASE (DECARBOXYLATING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02347">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycine cleavage system P-protein N-terminal" evidence="2">
        <location>
            <begin position="8"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="442" mass="47554" checksum="D06EC28D18218BC9" modified="2017-05-10" version="1">MTANGSPYAPHTDAETEAMLSAVGVESEAALFDIPDSVRFDDTFGIESRSERDVRSLVDRQLGRNHDLTEFLGRGHYDHYVPSMVDDLAGRSEFLTSYTQYQPEIAQGFLQALFEYQSMVAELTGLAIANCSMYDAATALGEAATLAIRVREADGDRVLVPEQLREGKRRTLENYLIGHDVSVATYPMADGIADVDALDAAAGEETLAVYAETPTVRGVIEERLDEIGAIATANDALFVVGSDPVALSLLEKPADVGADVVVGEAATLGLPAAYGTGMGLFACRESFLRQVPGRLVGASEDANGHRAYTLTLQTREQHIRRERATSNICTNQAWVALRTAMTIAWHGPDGLVDLAESCVVRAREAAERLDALDGVAAPVHDRHHFREFLARTDQPARAVADDLAAAGYAVHAVDDHLLQVCVTETNADAIDGLVEAVTEAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYR7</accession>
    <name>A0A1U7EYR7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">S8 family serine protease</fullName>
            <ecNumber evidence="9">3.4.21.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">NP_4628A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="9"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324019.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYR7"/>
    <dbReference type="STRING" id="348780.NP_4628A"/>
    <dbReference type="EnsemblBacteria" id="CAI50405">
        <property type="protein sequence ID" value="CAI50405"/>
        <property type="gene ID" value="NP_4628A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702123"/>
    <dbReference type="KEGG" id="nph:NP_4628A"/>
    <dbReference type="eggNOG" id="arCOG00702">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011263_16_1_2"/>
    <dbReference type="OrthoDB" id="219562at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07490">
        <property type="entry name" value="Peptidases_S8_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034190">
        <property type="entry name" value="Peptidase_S8_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF11">
        <property type="entry name" value="CEREVISIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5 6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S8/S53" evidence="8">
        <location>
            <begin position="194"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="445"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="203"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="240"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI50405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="575" mass="60084" checksum="A7A30A9B501E7045" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYS8</accession>
    <name>A0A1U7EYS8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">IS200-type transposase ISNph7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4630A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324020.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYS8"/>
    <dbReference type="STRING" id="348780.NP_4630A"/>
    <dbReference type="EnsemblBacteria" id="CAI50406">
        <property type="protein sequence ID" value="CAI50406"/>
        <property type="gene ID" value="NP_4630A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702124"/>
    <dbReference type="KEGG" id="nph:NP_4630A"/>
    <dbReference type="eggNOG" id="arCOG02759">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101320_2_0_2"/>
    <dbReference type="OrthoDB" id="92826at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1290">
        <property type="entry name" value="Transposase IS200-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002686">
        <property type="entry name" value="Transposase_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036515">
        <property type="entry name" value="Transposase_17_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033573">
        <property type="entry name" value="transpos_IS200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33360">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33360:SF2">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01797">
        <property type="entry name" value="Y1_Tnp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01321">
        <property type="entry name" value="Y1_Tnp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143422">
        <property type="entry name" value="Transposase IS200-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS200-like" evidence="1">
        <location>
            <begin position="10"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="149" mass="17331" checksum="4005D88BF2CB89B4" modified="2017-05-10" version="1">MGETRSNHTVYNINYHFVWCPKSSKSKICVPRGISDPAQYRHSVLDEIEQSLEASFKDVCGEYNYEIPSLHISPDHVHLFLSAHPKYSPSKIARKIKSITAREMWQQHEHLLENYLWGGGFWEESYYIGTAGEVSSQTIEQYIERTEHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYX8</accession>
    <name>A0A1U7EYX8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">TspO family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tspO</name>
        <name evidence="7" type="ordered locus">NP_4704A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TspO/BZRP family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50443.3"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939699.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYX8"/>
    <dbReference type="STRING" id="348780.NP_4704A"/>
    <dbReference type="EnsemblBacteria" id="CAI50443">
        <property type="protein sequence ID" value="CAI50443"/>
        <property type="gene ID" value="NP_4704A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703359"/>
    <dbReference type="KEGG" id="nph:NP_4704A"/>
    <dbReference type="eggNOG" id="arCOG04434">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091805_2_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033013">
        <property type="term" value="P:tetrapyrrole metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15904">
        <property type="entry name" value="TSPO_MBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.100:FF:000001">
        <property type="entry name" value="translocator protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.100">
        <property type="entry name" value="TspO/MBR protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038330">
        <property type="entry name" value="TspO/MBR-related_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004307">
        <property type="entry name" value="TspO_MBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10057">
        <property type="entry name" value="PERIPHERAL-TYPE BENZODIAZEPINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10057:SF0">
        <property type="entry name" value="TRANSLOCATOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03073">
        <property type="entry name" value="TspO_MBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005859">
        <property type="entry name" value="PBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI50443.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="160" mass="17453" checksum="2E133B5409FAD2ED" modified="2017-05-10" version="1">MLRRDDLTRRDIIVGVGAVLGVTALGSAPAVGVGSETGWFERPWFYPPELLFPVVWTLLFVLMGVAVAIVWLRGTDKRAVRVALSTFAAQFVLNLAWTPTFFGLQRPDLGLVVVGALWVAILATIAAFGRVSRLAAALLVPYLGWVSFAFVLNYAIYAAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ50</accession>
    <name>A0A1U7EZ50_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF8135 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_4936A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324171.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ50"/>
    <dbReference type="STRING" id="348780.NP_4936A"/>
    <dbReference type="EnsemblBacteria" id="CAI50559">
        <property type="protein sequence ID" value="CAI50559"/>
        <property type="gene ID" value="NP_4936A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702206"/>
    <dbReference type="KEGG" id="nph:NP_4936A"/>
    <dbReference type="eggNOG" id="arCOG07556">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1544246_0_0_2"/>
    <dbReference type="OrthoDB" id="204982at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058448">
        <property type="entry name" value="DUF8135"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26456">
        <property type="entry name" value="DUF8135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8135" evidence="2">
        <location>
            <begin position="114"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="173" mass="18443" checksum="BAA6F8C8DAA73990" modified="2017-05-10" version="1">MSEDADDAAAGTADESDDAPEAAVEENDDPEAAVEENDDPAVAVDESLAEFELEPADDESLEELFTEVETPDVDGEELWEEVLADPAEATSEPAEAAEAAVAGEGAAVDSGETVVQKDKYCQRCEYFAAPPELACEHPGTEIAAVLDSKQFRVVDCPIVARREHAKALFPDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZC2</accession>
    <name>A0A1U7EZC2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-directed RNA polymerase subunit Rpo7</fullName>
            <ecNumber evidence="4">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">DNA-directed RNA polymerase subunit E</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rpoE1</name>
        <name evidence="4" type="synonym">rpo7</name>
        <name evidence="4" type="synonym">rpoE</name>
        <name evidence="7" type="ordered locus">NP_5080A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the RNA polymerase complex. Forms a stalk with Rpo4 that extends from the main structure.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="4">Forms 2 domains with an elongated structure; Rpo4 packs into the hinge region between the 2 domains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324241.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZC2"/>
    <dbReference type="STRING" id="348780.NP_5080A"/>
    <dbReference type="EnsemblBacteria" id="CAI50631">
        <property type="protein sequence ID" value="CAI50631"/>
        <property type="gene ID" value="NP_5080A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703155"/>
    <dbReference type="KEGG" id="nph:NP_5080A"/>
    <dbReference type="eggNOG" id="arCOG00675">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117966_0_0_2"/>
    <dbReference type="OrthoDB" id="7927at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04331">
        <property type="entry name" value="RNAP_E_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04460">
        <property type="entry name" value="S1_RpoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.120">
        <property type="entry name" value="RNA polymerase Rpb7-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00865">
        <property type="entry name" value="RNApol_arch_Rpo7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036898">
        <property type="entry name" value="RNA_pol_Rpb7-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004519">
        <property type="entry name" value="RNAP_E/RPC8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046399">
        <property type="entry name" value="RNApol_Rpo7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045113">
        <property type="entry name" value="Rpb7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005576">
        <property type="entry name" value="Rpb7-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006333">
        <property type="entry name" value="PRK08563.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00448">
        <property type="entry name" value="rpoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709:SF4">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II, III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03876">
        <property type="entry name" value="SHS2_Rpb7-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88798">
        <property type="entry name" value="N-terminal, heterodimerisation domain of RBP7 (RpoE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4 7" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4 7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
            <begin position="82"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="156"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="174"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI50631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="189" mass="20952" checksum="0465BCAB71B943CA" modified="2017-05-10" version="1">MYKRVRLKDTVEVPPRHLADVSPELVKRLLQDKLEGQMDEDVGSIVSVTAVHDIGDGAVLPNRPGVYYEAEFDAVTFDPQMQEVVDGEVVEVVNFGAFVGIGPVDGLLHVSQISDEYLAYDEENQQLASRDSNRVLSVGDSVRARIVTKSIDERNPRDSKIGLTAKQPGLGKHGWLEEERQRREAQAGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZJ6</accession>
    <name>A0A1U7EZJ6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5 6">Ornithine carbamoyltransferase</fullName>
            <shortName evidence="5">OTCase</shortName>
            <ecNumber evidence="2 5 6">2.1.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 10" type="primary">argF</name>
        <name evidence="10" type="ordered locus">NP_5268A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>carbamoyl phosphate + L-ornithine = L-citrulline + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19513"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. OTCase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.3" evidence="2 5 6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324334.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZJ6"/>
    <dbReference type="STRING" id="348780.NP_5268A"/>
    <dbReference type="EnsemblBacteria" id="CAI50725">
        <property type="protein sequence ID" value="CAI50725"/>
        <property type="gene ID" value="NP_5268A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702520"/>
    <dbReference type="KEGG" id="nph:NP_5268A"/>
    <dbReference type="eggNOG" id="arCOG00912">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043846_3_2_2"/>
    <dbReference type="OrthoDB" id="4696at2157"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00112"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004585">
        <property type="term" value="F:ornithine carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019240">
        <property type="term" value="P:citrulline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000008">
        <property type="entry name" value="Ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01109">
        <property type="entry name" value="OTCase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002292">
        <property type="entry name" value="Orn/put_carbamltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024904">
        <property type="entry name" value="OTCase_ArgI"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00658">
        <property type="entry name" value="orni_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001986">
        <property type="entry name" value="PRK00779.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753">
        <property type="entry name" value="ORNITHINE CARBAMOYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753:SF3">
        <property type="entry name" value="ORNITHINE TRANSCARBAMYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00102">
        <property type="entry name" value="OTCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aspartate/ornithine carbamoyltransferase carbamoyl-P binding" evidence="9">
        <location>
            <begin position="2"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Aspartate/ornithine carbamoyltransferase Asp/Orn-binding" evidence="8">
        <location>
            <begin position="145"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="47"/>
            <end position="50"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="125"/>
            <end position="128"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="219"/>
            <end position="220"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="253"/>
            <end position="254"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="NCBIfam" id="TIGR00658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI50725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="294" mass="31811" checksum="0AA8CEF5FF6552F8" modified="2017-05-10" version="1">MNFLNIDDLTREQLETVLTEAERLKAGETTADAMAGDTMAMIFEKPSTRTRVSFETGATQLGGHAIFLGPDDIHLGHGEPVKDTARALSRYVDVIMARVFDHADVEELGEYATVPVVNGLTDDAHPCQTLADLLTIREQFGGFDATVAWVGDGNNVCQSFVLGAAMVGLDVVVATPEAYSVDDAVLERAAELGGEPETTTDPEAAVDGADAVYTDVWVSMGEESEREEKLSVFESDGFRITEELLGDRPLLHCLPAHRGEEVTDAAIESDNAIVWDQAENRLHAQKGLLTYLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZR1</accession>
    <name>A0A1U7EZR1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SpoVT-AbrB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NP_3713F</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CDN71458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_083761678.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZR1"/>
    <dbReference type="EnsemblBacteria" id="CDN71458">
        <property type="protein sequence ID" value="CDN71458"/>
        <property type="gene ID" value="NP_3713F"/>
    </dbReference>
    <dbReference type="GeneID" id="32155015"/>
    <dbReference type="KEGG" id="nph:NP_3713F"/>
    <dbReference type="eggNOG" id="arCOG00818">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3302664_0_0_2"/>
    <dbReference type="OrthoDB" id="67352at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01439">
        <property type="entry name" value="lp_hng_hel_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04014">
        <property type="entry name" value="MazE_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89447">
        <property type="entry name" value="AbrB/MazE/MraZ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="1">
        <location>
            <begin position="13"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CDN71458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="42" mass="4850" checksum="CA882E29BC3E8666" modified="2017-05-10" version="1">MGQMECYPKMRQRGVVTIPEEVRDGLNLEEGDQLKLTVEKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM35</accession>
    <name>Q3IM35_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphate transport system permease protein PstA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">pstA2</name>
        <name evidence="10" type="synonym">abc15p1</name>
        <name evidence="10" type="ordered locus">NP_6100A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10 11">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324432.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM35"/>
    <dbReference type="EnsemblBacteria" id="CAI50830">
        <property type="protein sequence ID" value="CAI50830"/>
        <property type="gene ID" value="NP_6100A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694670"/>
    <dbReference type="KEGG" id="nph:NP_6100A"/>
    <dbReference type="HOGENOM" id="CLU_033621_2_1_2"/>
    <dbReference type="OrthoDB" id="11402at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005672">
        <property type="entry name" value="Phosphate_PstA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00974">
        <property type="entry name" value="3a0107s02c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43470:SF5">
        <property type="entry name" value="PHOSPHATE TRANSPORT SYSTEM PERMEASE PROTEIN PSTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43470">
        <property type="entry name" value="PHOSPHATE TRANSPORT SYSTEM PERMEASE PROTEIN PSTA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="86"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="124"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="270"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="87"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI50830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="303" mass="32625" checksum="02240CBC0F59D141" modified="2005-11-08" version="1">MSTPADTPTGEGLFDDVDLGWEHLKNRLFKYLLFGAAMFGVVALGVLIFDVITDFYVGLTEFNISLVDFVTRDGSRRPELSGFRGAIVASVMLMALTAFLSFFIGVGSAIYLEEYAPDNRITRLIEANLANLAGVPSIVYGLLALAALRNGIGLGPIVLVGGVALALLVMPIIIVSSQEALRAVPDSVRRGSYATGATQWQTIRKVVLPAALPGIFTGTILALARAIGETAPLLMVGALFVNRMPRGLMDRFSGMPTQIYSWAAEPSQHFIHLAAVGIVVLLTFMLVMNGIAVYLRNKYETEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM58</accession>
    <name>Q3IM58_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_6046A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50803.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM58"/>
    <dbReference type="EnsemblBacteria" id="CAI50803">
        <property type="protein sequence ID" value="CAI50803"/>
        <property type="gene ID" value="NP_6046A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_6046A"/>
    <dbReference type="HOGENOM" id="CLU_1458234_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50803.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="185" mass="20771" checksum="5DA0287B5836DF2B" modified="2005-11-08" version="1">MNIQLDPGVILTTTARHTHHAVMFNEDWQAITRDGDFVSTLRAVETEPDCAEIDADTVSNIVDALETHSVFATNLNDHERDVINAIELYADGLYSFDASIQWSYFWFCIENLLGEGRGSGRDTKNHLVQQDLLSTEDADKWKSAVDRIKHPDKGVDSDMTDISLPSAFKCREVASEVLLGRLTDR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMV2</accession>
    <name>ILVD_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dihydroxy-acid dehydratase</fullName>
            <shortName evidence="1">DAD</shortName>
            <ecNumber evidence="1">4.2.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ilvD</name>
        <name type="ordered locus">NP_4926A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Functions in the biosynthesis of branched-chain amino acids. Catalyzes the dehydration of (2R,3R)-2,3-dihydroxy-3-methylpentanoate (2,3-dihydroxy-3-methylvalerate) into 2-oxo-3-methylpentanoate (2-oxo-3-methylvalerate) and of (2R)-2,3-dihydroxy-3-methylbutanoate (2,3-dihydroxyisovalerate) into 2-oxo-3-methylbutanoate (2-oxoisovalerate), the penultimate precursor to L-isoleucine and L-valine, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:24809"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:24810"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate = (S)-3-methyl-2-oxopentanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:27694"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:27695"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="1">Binds 1 [2Fe-2S] cluster per subunit. This cluster acts as a Lewis acid cofactor.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the IlvD/Edd family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.9" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50554.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324166.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMV2"/>
    <dbReference type="SMR" id="Q3IMV2"/>
    <dbReference type="STRING" id="348780.NP_4926A"/>
    <dbReference type="EnsemblBacteria" id="CAI50554">
        <property type="protein sequence ID" value="CAI50554"/>
        <property type="gene ID" value="NP_4926A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702868"/>
    <dbReference type="KEGG" id="nph:NP_4926A"/>
    <dbReference type="eggNOG" id="arCOG04045">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014271_4_2_2"/>
    <dbReference type="OrthoDB" id="8674at2157"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00057"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00061"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004160">
        <property type="term" value="F:dihydroxy-acid dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.80:FF:000001">
        <property type="entry name" value="Dihydroxy-acid dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.80">
        <property type="entry name" value="IlvD/EDD C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00012">
        <property type="entry name" value="IlvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050165">
        <property type="entry name" value="DHAD_IlvD/Edd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042096">
        <property type="entry name" value="Dihydro-acid_dehy_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004404">
        <property type="entry name" value="DihydroxyA_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020558">
        <property type="entry name" value="DiOHA_6PGluconate_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056740">
        <property type="entry name" value="ILV_EDD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000581">
        <property type="entry name" value="ILV_EDD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037237">
        <property type="entry name" value="IlvD/EDD_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00110">
        <property type="entry name" value="ilvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002068">
        <property type="entry name" value="PRK00911.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21000">
        <property type="entry name" value="DIHYDROXY-ACID DEHYDRATASE DAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21000:SF5">
        <property type="entry name" value="DIHYDROXY-ACID DEHYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24877">
        <property type="entry name" value="ILV_EDD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00920">
        <property type="entry name" value="ILVD_EDD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143975">
        <property type="entry name" value="IlvD/EDD N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00886">
        <property type="entry name" value="ILVD_EDD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00887">
        <property type="entry name" value="ILVD_EDD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0001">2Fe-2S</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000225441" description="Dihydroxy-acid dehydratase">
        <location>
            <begin position="1"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="22"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="501"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="via carbamate group" evidence="1">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="475"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="1">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="593" mass="62779" checksum="0A2D44AA220C24CB" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMY2</accession>
    <name>RS3_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein uS3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">30S ribosomal protein S3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps3</name>
        <name type="ordered locus">NP_4866A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds the lower part of the 30S subunit head.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324136.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMY2"/>
    <dbReference type="SMR" id="Q3IMY2"/>
    <dbReference type="STRING" id="348780.NP_4866A"/>
    <dbReference type="EnsemblBacteria" id="CAI50524">
        <property type="protein sequence ID" value="CAI50524"/>
        <property type="gene ID" value="NP_4866A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703180"/>
    <dbReference type="KEGG" id="nph:NP_4866A"/>
    <dbReference type="eggNOG" id="arCOG04097">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058591_1_0_2"/>
    <dbReference type="OrthoDB" id="9126at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02411">
        <property type="entry name" value="KH-II_30S_S3_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.20:FF:000001">
        <property type="entry name" value="30S ribosomal protein S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.32">
        <property type="entry name" value="Ribosomal protein S3, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01309_A">
        <property type="entry name" value="Ribosomal_uS3_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036419">
        <property type="entry name" value="Ribosomal_S3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057258">
        <property type="entry name" value="Ribosomal_uS3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027488">
        <property type="entry name" value="Ribosomal_uS3_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001351">
        <property type="entry name" value="Ribosomal_uS3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018280">
        <property type="entry name" value="Ribosomal_uS3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005703">
        <property type="entry name" value="Ribosomal_uS3_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003219">
        <property type="entry name" value="PRK04191.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01008">
        <property type="entry name" value="uS3_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760">
        <property type="entry name" value="30S/40S RIBOSOMAL PROTEIN S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760:SF32">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00189">
        <property type="entry name" value="Ribosomal_S3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="Prokaryotic type KH domain (KH-domain type II)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54821">
        <property type="entry name" value="Ribosomal protein S3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50823">
        <property type="entry name" value="KH_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00548">
        <property type="entry name" value="RIBOSOMAL_S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000230746" description="Small ribosomal subunit protein uS3">
        <location>
            <begin position="1"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="KH type-2" evidence="1">
        <location>
            <begin position="17"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="207"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="207"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="272"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="305" mass="33393" checksum="D6681C14369CECB3" modified="2005-11-08" version="1">MADEHQFIHDGLQRTQIDEFFSEELSRAGYGGMEVAKTPMGTQIVLKAEKPGMVIGKGGKNIRKITTTLEEEFGLEDPQVDVQEVDEPDLNAQIVADRLANALERGWYFRKAGHTTIDRIMESGALGAEIILSGKVTGARSRVEKFNRGYIKHNGEPAESIVDSGVGTAVMKLGTIGVQVKIIPPEAELPDDFEVYEDIEVEDFVEEPEGDVEELLEAPEDAEAESVGADPEEVIEEETEAEAVEEVAEEEIIDEEGVEAETGDAPTSPPDAAEEPDEALDEDVEAEAEELLDEMEDETTDEEET</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-30" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMY5</accession>
    <name>RL2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">50S ribosomal protein L2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl2</name>
        <name type="ordered locus">NP_4860A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Forms a bridge to the 30S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324133.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMY5"/>
    <dbReference type="SMR" id="Q3IMY5"/>
    <dbReference type="STRING" id="348780.NP_4860A"/>
    <dbReference type="EnsemblBacteria" id="CAI50521">
        <property type="protein sequence ID" value="CAI50521"/>
        <property type="gene ID" value="NP_4860A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703130"/>
    <dbReference type="KEGG" id="nph:NP_4860A"/>
    <dbReference type="eggNOG" id="arCOG04067">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036235_0_1_2"/>
    <dbReference type="OrthoDB" id="5987at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000001">
        <property type="entry name" value="50S ribosomal protein L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000020">
        <property type="entry name" value="60S ribosomal protein L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.950.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.950.10">
        <property type="entry name" value="Ribosomal protein L2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01320_A">
        <property type="entry name" value="Ribosomal_uL2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002171">
        <property type="entry name" value="Ribosomal_uL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023672">
        <property type="entry name" value="Ribosomal_uL2_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022669">
        <property type="entry name" value="Ribosomal_uL2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022671">
        <property type="entry name" value="Ribosomal_uL2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014726">
        <property type="entry name" value="Ribosomal_uL2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022666">
        <property type="entry name" value="Ribosomal_uL2_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007180">
        <property type="entry name" value="PRK09612.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691">
        <property type="entry name" value="RIBOSOMAL PROTEIN L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03947">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00181">
        <property type="entry name" value="Ribosomal_L2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002158">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01383">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01382">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00467">
        <property type="entry name" value="RIBOSOMAL_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000237293" description="Large ribosomal subunit protein uL2">
        <location>
            <begin position="1"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="198"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="221"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="243" mass="25775" checksum="8C0B00E05B6E0B77" modified="2005-11-08" version="1">MGRRILGQRRGRGTSTFRAPSHRYKADLSHRNVEESDLVTGEVVDIEHDPARSAPLADVQFDDGDRRLVLAPEGVTVGDEIQIGVSAEIAPGNTMPLAEIPEGVPVCNVERQPGDGGKFARASGVSATLLTHDRNAAVVQLPSGEMRRLSPECRATIGVVAGGGRTEKPFVKAGNKHHKMKSRGGKWPRVRGVAMNAVDHPFGGGGRQHPGKPKSVSRDTPPGRKVGDIASKRTGRGGKGGQE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IN38</accession>
    <name>IF5A_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Translation initiation factor 5A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Hypusine-containing protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">eIF-5A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">eif5a</name>
        <name type="ordered locus">NP_4752A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Functions by promoting the formation of the first peptide bond.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-5A family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324080.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IN38"/>
    <dbReference type="SMR" id="Q3IN38"/>
    <dbReference type="STRING" id="348780.NP_4752A"/>
    <dbReference type="EnsemblBacteria" id="CAI50467">
        <property type="protein sequence ID" value="CAI50467"/>
        <property type="gene ID" value="NP_4752A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703270"/>
    <dbReference type="KEGG" id="nph:NP_4752A"/>
    <dbReference type="eggNOG" id="arCOG04277">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102600_3_0_2"/>
    <dbReference type="OrthoDB" id="23689at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045901">
        <property type="term" value="P:positive regulation of translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045905">
        <property type="term" value="P:positive regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04467">
        <property type="entry name" value="S1_aIF5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000038">
        <property type="entry name" value="Translation initiation factor 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00085">
        <property type="entry name" value="eIF_5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001884">
        <property type="entry name" value="IF5A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048670">
        <property type="entry name" value="IF5A-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020189">
        <property type="entry name" value="IF5A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019769">
        <property type="entry name" value="Trans_elong_IF5A_hypusine_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022847">
        <property type="entry name" value="Transl_elong_IF5A_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00037">
        <property type="entry name" value="eIF_5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003076">
        <property type="entry name" value="PRK03999.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11673">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR 5A FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21485">
        <property type="entry name" value="IF5A-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003025">
        <property type="entry name" value="eIF5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01376">
        <property type="entry name" value="eIF-5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00302">
        <property type="entry name" value="IF5A_HYPUSINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0385">Hypusine</keyword>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000259441" description="Translation initiation factor 5A">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="27"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="modified residue" description="Hypusine" evidence="1">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="124" mass="14008" checksum="EF1A567638535593" modified="2005-11-08" version="1">MPKEQKEVRDLQEGNYVMIEDVPSKITSYSTSKPGKHGSAKARVEGTGVFDGQKRNFTQPVDAKVWVPIVNRKQGQVVSVSGDDMQVMDLETYETITMRIPEDLAPESDDEIEYLEFEGQRKVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q3INB7</accession>
    <name>Q3INB7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable membrane transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">NP_4590A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324001.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3INB7"/>
    <dbReference type="STRING" id="348780.NP_4590A"/>
    <dbReference type="EnsemblBacteria" id="CAI50386">
        <property type="protein sequence ID" value="CAI50386"/>
        <property type="gene ID" value="NP_4590A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702107"/>
    <dbReference type="KEGG" id="nph:NP_4590A"/>
    <dbReference type="eggNOG" id="arCOG02050">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045498_0_1_2"/>
    <dbReference type="OrthoDB" id="203751at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43483">
        <property type="entry name" value="MEMBRANE TRANSPORTER PROTEIN HI_0806-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43483:SF3">
        <property type="entry name" value="MEMBRANE TRANSPORTER PROTEIN HI_0806-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="233"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="305"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI50386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="336" mass="33835" checksum="41774FDC9327053A" modified="2005-11-08" version="1">MELLGLTPQLAALFVGFGFMVGVFFGFFGMGGSFLVTPTLLILGYPAPVAIGSSMAFVFGTAVIATLKHHDVGQVDYKLGALMFVGIAVGIEAGKMLVFFLEALGRAEVVVGSAYVVLLAAIGGVFVRSAAGGDSADSADSEGGDGSEDDIPDIAKKIKSYTIPPMVTLTDGSKASMWTISGVGGGVGVVSGFLGVGGGFIRMPAIYYIIGVPLAAAVGTSLFGALMSGAVGAFTYGLSGVIDLGVVTALLVGSALGARIGSAATAYVDEDDVTIYFGVMLLLASIAVAAGELATWLDAPVLDTVSFVLLVGSAAFVTAVIFYYGATAVRNGTRVG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-09-23" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q3INH7</accession>
    <name>DGGGP_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Digeranylgeranylglyceryl phosphate synthase</fullName>
            <shortName evidence="1">DGGGP synthase</shortName>
            <shortName evidence="1">DGGGPS</shortName>
            <ecNumber evidence="1">2.5.1.42</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">(S)-2,3-di-O-geranylgeranylglyceryl phosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Geranylgeranylglycerol-phosphate geranylgeranyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">NP_4470A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Prenyltransferase that catalyzes the transfer of the geranylgeranyl moiety of geranylgeranyl diphosphate (GGPP) to the C2 hydroxyl of (S)-3-O-geranylgeranylglyceryl phosphate (GGGP). This reaction is the second ether-bond-formation step in the biosynthesis of archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>sn-3-O-(geranylgeranyl)glycerol 1-phosphate + (2E,6E,10E)-geranylgeranyl diphosphate = 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:18109"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57677"/>
            <dbReference type="ChEBI" id="CHEBI:58756"/>
            <dbReference type="ChEBI" id="CHEBI:58837"/>
            <dbReference type="EC" id="2.5.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UbiA prenyltransferase family. DGGGP synthase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.42" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323941.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3INH7"/>
    <dbReference type="SMR" id="Q3INH7"/>
    <dbReference type="STRING" id="348780.NP_4470A"/>
    <dbReference type="EnsemblBacteria" id="CAI50326">
        <property type="protein sequence ID" value="CAI50326"/>
        <property type="gene ID" value="NP_4470A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703434"/>
    <dbReference type="KEGG" id="nph:NP_4470A"/>
    <dbReference type="eggNOG" id="arCOG00476">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073311_1_1_2"/>
    <dbReference type="OrthoDB" id="11851at2157"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047295">
        <property type="term" value="F:geranylgeranylglycerol-phosphate geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13961">
        <property type="entry name" value="PT_UbiA_DGGGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1780">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01286">
        <property type="entry name" value="DGGGP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023547">
        <property type="entry name" value="DGGGP_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050475">
        <property type="entry name" value="Prenyltransferase_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009521">
        <property type="entry name" value="PRK12882.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42723">
        <property type="entry name" value="CHLOROPHYLL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42723:SF1">
        <property type="entry name" value="CHLOROPHYLL SYNTHASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000350714" description="Digeranylgeranylglyceryl phosphate synthase">
        <location>
            <begin position="1"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01286"/>
        </source>
    </evidence>
    <sequence length="277" mass="28508" checksum="4097BBBE8CBCE568" modified="2005-11-08" version="1">MERVRGLVELLRPGNAVAAGGLTFIGAFVAGGLSSPQSMAFAVVATVLATGAGNAINDYFDRDIDAINEPDRPIPRGAVSPRGALVYSVALFAVAVVLTLLLPWLAIAIAAINLVALVAYTEVFKGLPGVGNALVAYLTGSTFLYGGAAVGGDLAAVVVLFALAACATMAREIVKDVEDIDGDRAEGLRTLPIVIGERRSLYVAAGFVVVAVLSSPLPYLLGLFGWVYLVVLVPALCGLAAATWRSFSDPTTGQAWLKASMFAAAVAFVIGRLAVVA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-09-22" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IP35</accession>
    <name>THIM_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hydroxyethylthiazole kinase</fullName>
            <ecNumber evidence="1">2.7.1.50</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">4-methyl-5-beta-hydroxyethylthiazole kinase</fullName>
            <shortName evidence="1">TH kinase</shortName>
            <shortName evidence="1">Thz kinase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thiM</name>
        <name type="ordered locus">NP_4052A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-(2-hydroxyethyl)-4-methylthiazole + ATP = 4-methyl-5-(2-phosphooxyethyl)-thiazole + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24212"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17957"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58296"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.50"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Thz kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.50" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323733.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IP35"/>
    <dbReference type="SMR" id="Q3IP35"/>
    <dbReference type="STRING" id="348780.NP_4052A"/>
    <dbReference type="EnsemblBacteria" id="CAI50117">
        <property type="protein sequence ID" value="CAI50117"/>
        <property type="gene ID" value="NP_4052A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703251"/>
    <dbReference type="KEGG" id="nph:NP_4052A"/>
    <dbReference type="eggNOG" id="arCOG00019">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019943_0_1_2"/>
    <dbReference type="OrthoDB" id="214286at2157"/>
    <dbReference type="UniPathway" id="UPA00060">
        <property type="reaction ID" value="UER00139"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004417">
        <property type="term" value="F:hydroxyethylthiazole kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01170">
        <property type="entry name" value="THZ_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00228">
        <property type="entry name" value="Thz_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000417">
        <property type="entry name" value="Hyethyz_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006830">
        <property type="entry name" value="PRK09355.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02110">
        <property type="entry name" value="HK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000513">
        <property type="entry name" value="Thz_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01099">
        <property type="entry name" value="HYETHTZKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000383922" description="Hydroxyethylthiazole kinase">
        <location>
            <begin position="1"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00228"/>
        </source>
    </evidence>
    <sequence length="278" mass="28228" checksum="A3923D1647630C7C" modified="2005-11-08" version="1">MSDVDAAVDLAAALGDIDTATPLVNSVTNNVTVNDVANITLHWGGLPVMSDDAREVDDMVATADGCLINMGTVDEDGEESMVVAGTAANEGDKPLVVDPVGVGATPTRTRVANRLLDELDVTILNGNHGEITALAGDDADVRGVESVGEYADIAETALSCARTHDTVVVASGETDIVASPEEAYEITAGHPLMAQIVGTGCMLGVTLATFAAGMENATPLDAALAGTVGFGLAGEKAAEEGEYNGPASYKTAFLDTVAGLEAAPDDPAGRIERVLSVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IQ90</accession>
    <name>Q3IQ90_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Probable archaetidylserine synthase</fullName>
            <ecNumber evidence="4">2.7.8.38</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">assA</name>
        <name evidence="4" type="synonym">pgsA3</name>
        <name evidence="4" type="ordered locus">NP_3230A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
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        <property type="protein sequence ID" value="CAI49706.1"/>
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    <dbReference type="EnsemblBacteria" id="CAI49706">
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        <property type="gene ID" value="NP_3230A"/>
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    <dbReference type="GeneID" id="3703065"/>
    <dbReference type="KEGG" id="nph:NP_3230A"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:archaetidylserine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
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        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038086">
        <property type="entry name" value="anchor_synt_A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2 4" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="132"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="182"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="205"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="231" mass="23724" checksum="88966F8B6E714735" modified="2005-11-08" version="1">MQPRFLGQLGLADVVTVANAALGFIAAAVAVFDTELAARLILLAAIADGLDGVIARFRGGTPVGPILDSLADVASFGVAPAVLVYALASGGEVTLDEPLAVAAVVVAALYVAMAVVRLGFYLLEDDGAPETVGVQTTLAATVLSVLHLAGVTDPAVFVAATAAFAYLMVASITYPELYARDALILGGLQACVVLFPTAFGRAFPRVLLVFALGYLLLAPVAYWRSTEGKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IQF1</accession>
    <name>Q3IQF1_NATPD</name>
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        <submittedName>
            <fullName evidence="9">Type IV pilus biogenesis complex membrane subunit</fullName>
        </submittedName>
    </protein>
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        <name evidence="9" type="primary">pilC1</name>
        <name evidence="9" type="ordered locus">NP_3108A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323267.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IQF1"/>
    <dbReference type="STRING" id="348780.NP_3108A"/>
    <dbReference type="EnsemblBacteria" id="CAI49645">
        <property type="protein sequence ID" value="CAI49645"/>
        <property type="gene ID" value="NP_3108A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701655"/>
    <dbReference type="KEGG" id="nph:NP_3108A"/>
    <dbReference type="eggNOG" id="arCOG01808">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017646_0_0_2"/>
    <dbReference type="OrthoDB" id="12374at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.81.30">
        <property type="entry name" value="Type II secretion system (T2SS), domain F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056569">
        <property type="entry name" value="ArlJ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018076">
        <property type="entry name" value="T2SS_GspF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042094">
        <property type="entry name" value="T2SS_GspF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35402">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35402:SF1">
        <property type="entry name" value="TYPE II SECRETION SYSTEM PROTEIN GSPF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00482">
        <property type="entry name" value="T2SSF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="115"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="275"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="302"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="397"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="431"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="569"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="623"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="domain" description="Type II secretion system protein GspF" evidence="8">
        <location>
            <begin position="167"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Type II secretion system protein GspF" evidence="8">
        <location>
            <begin position="464"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="244"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI49645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="672" mass="73138" checksum="3B2AFCBF965798F6" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-04-08" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IRC9</accession>
    <name>COXX1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName>Protoheme IX farnesyltransferase 1</fullName>
            <ecNumber>2.5.1.141</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Heme B farnesyltransferase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Heme O synthase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">ctaB1</name>
        <name type="ordered locus">NP_2446A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>heme b + (2E,6E)-farnesyl diphosphate + H2O = Fe(II)-heme o + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:28070"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="ChEBI" id="CHEBI:60530"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.141"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Porphyrin-containing compound metabolism; heme O biosynthesis; heme O from protoheme: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Carbon 2 of the heme B porphyrin ring is defined according to the Fischer nomenclature.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the UbiA prenyltransferase family. Protoheme IX farnesyltransferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.141"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322940.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IRC9"/>
    <dbReference type="SMR" id="Q3IRC9"/>
    <dbReference type="STRING" id="348780.NP_2446A"/>
    <dbReference type="EnsemblBacteria" id="CAI49314">
        <property type="protein sequence ID" value="CAI49314"/>
        <property type="gene ID" value="NP_2446A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702642"/>
    <dbReference type="KEGG" id="nph:NP_2446A"/>
    <dbReference type="eggNOG" id="arCOG00479">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030009_1_1_2"/>
    <dbReference type="OrthoDB" id="131615at2157"/>
    <dbReference type="UniPathway" id="UPA00834">
        <property type="reaction ID" value="UER00712"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008495">
        <property type="term" value="F:protoheme IX farnesyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048034">
        <property type="term" value="P:heme O biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13957">
        <property type="entry name" value="PT_UbiA_Cox10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1780">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00154">
        <property type="entry name" value="CyoE_CtaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006369">
        <property type="entry name" value="Protohaem_IX_farnesylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01473">
        <property type="entry name" value="cyoE_ctaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003349">
        <property type="entry name" value="PRK04375.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43448">
        <property type="entry name" value="PROTOHEME IX FARNESYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43448:SF2">
        <property type="entry name" value="PROTOHEME IX FARNESYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0350">Heme biosynthesis</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000327204" description="Protoheme IX farnesyltransferase 1">
        <location>
            <begin position="1"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="251"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="276"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="300"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="321"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="377"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="398"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="433"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Unknown">
        <location>
            <begin position="1"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="137"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Protoheme IX prenyltransferase">
        <location>
            <begin position="187"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="454" mass="46819" checksum="6D7B6240C3F5AC27" modified="2005-11-08" version="1">MRTTGFSGLLSATVVGVYVLVVVGATAALADAAAACQGWPLCGGDLSDPSVPVAVLHRGVAAVVGLLVIATAVVGWSETTARVKTTLALALALYPAQVVLGALVATGSALANLHLFVAMAIFAVLVVGLAWHLEAETGSDDAPESPPELAPPVDEEPAATEQPALSGLARAKATARAYFELTKPRLMWLLCLVAGAGMVIAGTPTVRTVVFTLGGGVLAIGASGTFNHVLERDIDRRMERTSDRPIATHEVPVANALAFGGLLAVASLWAFLSVNLLAAALGLAAIAFYSVVYTLILKPNTVQNTVIGGAAGALPALIGYAAVTGTIGIGGLVLAAVIFLWTPAHFYNLALAYKDDYERGGFPMMPVVRGETETRKHIVFYLGATLLGAGALAAVTDLGWLYAATAVLAAGVFLWAVVELHYEQTDRAAFRSFHASNAYLGLVLVAILIDSLAV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IRY6</accession>
    <name>COFD_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">2-phospho-L-lactate transferase</fullName>
            <ecNumber evidence="1">2.7.8.28</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cofD</name>
        <name type="ordered locus">NP_2028A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of the 2-phospholactate moiety from (2S)-lactyl-2-diphospho-5'-guanosine to 7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO) with the formation of oxidized coenzyme F420-0 and GMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2S)-lactyl-2-diphospho-5'-guanosine + 7,8-didemethyl-8-hydroxy-5-deazariboflavin = oxidized coenzyme F420-0 + GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:63444"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:59435"/>
            <dbReference type="ChEBI" id="CHEBI:59904"/>
            <dbReference type="ChEBI" id="CHEBI:59907"/>
            <dbReference type="EC" id="2.7.8.28"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme F420 biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CofD family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.28" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322734.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IRY6"/>
    <dbReference type="SMR" id="Q3IRY6"/>
    <dbReference type="STRING" id="348780.NP_2028A"/>
    <dbReference type="EnsemblBacteria" id="CAI49105">
        <property type="protein sequence ID" value="CAI49105"/>
        <property type="gene ID" value="NP_2028A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701331"/>
    <dbReference type="KEGG" id="nph:NP_2028A"/>
    <dbReference type="eggNOG" id="arCOG04395">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055795_1_0_2"/>
    <dbReference type="OrthoDB" id="59563at2157"/>
    <dbReference type="UniPathway" id="UPA00071"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043743">
        <property type="term" value="F:LPPG:FO 2-phospho-L-lactate transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052645">
        <property type="term" value="P:F420-0 metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07186">
        <property type="entry name" value="CofD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10680">
        <property type="entry name" value="CofD-like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01257">
        <property type="entry name" value="CofD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002882">
        <property type="entry name" value="CofD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038136">
        <property type="entry name" value="CofD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010115">
        <property type="entry name" value="FbiA/CofD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01819">
        <property type="entry name" value="F420_cofD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43007">
        <property type="entry name" value="2-PHOSPHO-L-LACTATE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43007:SF1">
        <property type="entry name" value="2-PHOSPHO-L-LACTATE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01933">
        <property type="entry name" value="CofD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142338">
        <property type="entry name" value="CofD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000259480" description="2-phospho-L-lactate transferase">
        <location>
            <begin position="1"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>7,8-didemethyl-8-hydroxy-5-deazariboflavin</name>
            <dbReference type="ChEBI" id="CHEBI:59904"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01257"/>
        </source>
    </evidence>
    <sequence length="334" mass="35792" checksum="E62B29A122C81042" modified="2005-11-08" version="1">MVTFLAGGTGTPKLRYGAETVFDTADTPVVCNTGDDVELGGLVVCPDVDTVLFAAADRLDRETWWGIDGDTTETHEELRALADAADLDTGPRYLPDKKQTAGRDIARWRRFSGVAEFMEIGDTDRAVHITRTSLLDEGKRLTEATATLADALGVDHPVLPMSDDPVATLIDTDEGLVHFQEFWVHRRAEPTINGVEFRGADAAEPTPEVREALADPVVIGPSNPITSIGPMVALDGIRKALAETPVVAVSPFVEDRVFSGPADDLMAADGYAPSTAGVAEAYPFADAFVVDEADETPLERPTVRTDTELGSPADGERVCRAVREAFDRLGVEVA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ISB2</accession>
    <name>RS142_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein uS14B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">30S ribosomal protein S14 type Z 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps14b</name>
        <name evidence="1" type="synonym">rps142</name>
        <name type="ordered locus">NP_1768A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds 16S rRNA, required for the assembly of 30S particles.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS14 family. Zinc-binding uS14 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Unlike some other archaeal S14P proteins, this is unable to bind zinc.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322608.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ISB2"/>
    <dbReference type="SMR" id="Q3ISB2"/>
    <dbReference type="STRING" id="348780.NP_1768A"/>
    <dbReference type="EnsemblBacteria" id="CAI48975">
        <property type="protein sequence ID" value="CAI48975"/>
        <property type="gene ID" value="NP_1768A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703170"/>
    <dbReference type="KEGG" id="nph:NP_1768A"/>
    <dbReference type="eggNOG" id="arCOG00782">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_177289_2_1_2"/>
    <dbReference type="OrthoDB" id="5615at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.10">
        <property type="entry name" value="30s Ribosomal Protein S14, Chain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01364_A">
        <property type="entry name" value="Ribosomal_uS14_2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001209">
        <property type="entry name" value="Ribosomal_uS14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023676">
        <property type="entry name" value="Ribosomal_uS14_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039744">
        <property type="entry name" value="RIbosomal_uS14_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043140">
        <property type="entry name" value="Ribosomal_uS14_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004424">
        <property type="entry name" value="PRK05766.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12010">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12010:SF2">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00253">
        <property type="entry name" value="Ribosomal_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000269169" description="Small ribosomal subunit protein uS14B">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="49" mass="5812" checksum="76B3D6D61D990CCE" modified="2005-11-08" version="1">MGAQDPTDEDRHVCRDTGRKQGLVGKYDIWLCRQSFREMARDMGFKKYD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ISC7</accession>
    <name>PGP_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoglycolate phosphatase</fullName>
            <shortName evidence="1">PGP</shortName>
            <shortName evidence="1">PGPase</shortName>
            <ecNumber evidence="1">3.1.3.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">NP_1738A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the dephosphorylation of 2-phosphoglycolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-phosphoglycolate + H2O = glycolate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14369"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29805"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58033"/>
            <dbReference type="EC" id="3.1.3.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal SPP-like hydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.18" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322593.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ISC7"/>
    <dbReference type="SMR" id="Q3ISC7"/>
    <dbReference type="STRING" id="348780.NP_1738A"/>
    <dbReference type="EnsemblBacteria" id="CAI48960">
        <property type="protein sequence ID" value="CAI48960"/>
        <property type="gene ID" value="NP_1738A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701244"/>
    <dbReference type="KEGG" id="nph:NP_1738A"/>
    <dbReference type="eggNOG" id="arCOG01213">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044146_2_0_2"/>
    <dbReference type="OrthoDB" id="120822at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008967">
        <property type="term" value="F:phosphoglycolate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01427">
        <property type="entry name" value="HAD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07514">
        <property type="entry name" value="HAD_Pase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1070.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01419">
        <property type="entry name" value="GPH_hydrolase_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006382">
        <property type="entry name" value="PGPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01487">
        <property type="entry name" value="Pglycolate_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10000:SF8">
        <property type="entry name" value="HAD SUPERFAMILY HYDROLASE-LIKE, TYPE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10000">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08282">
        <property type="entry name" value="Hydrolase_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000024293" description="Phosphoglycolate phosphatase">
        <location>
            <begin position="1"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="8"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01419"/>
        </source>
    </evidence>
    <sequence length="226" mass="23777" checksum="8741CF45E5384F52" modified="2005-11-08" version="1">MVAPLAVDIDGTLTRPDKSIDPRVFDAIRAWDDHVVIATGKSFPYPVGLCEFLGMPLNVIAENGGAVYVEPAGEVVYNGDPEGAAAVAEEYVAAGYDLGWGAVDMVNRWRETELAVDRDQPLEPLVAIADDHGMDVVDTGYAYHVKDAGVDKATGLETVAELLGVVPSSFIAIGDSENDAELLELAGTGFAVANADAHARGAADAVTDASFADGFLEALDRARDNR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ISC9</accession>
    <name>PYRF_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Orotidine 5'-phosphate decarboxylase</fullName>
            <ecNumber evidence="1">4.1.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">OMP decarboxylase</fullName>
            <shortName evidence="1">OMPDCase</shortName>
            <shortName evidence="1">OMPdecase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrF</name>
        <name type="ordered locus">NP_1734A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>orotidine 5'-phosphate + H(+) = UMP + CO2</text>
            <dbReference type="Rhea" id="RHEA:11596"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="EC" id="4.1.1.23"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the OMP decarboxylase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.23" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322591.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ISC9"/>
    <dbReference type="SMR" id="Q3ISC9"/>
    <dbReference type="STRING" id="348780.NP_1734A"/>
    <dbReference type="EnsemblBacteria" id="CAI48958">
        <property type="protein sequence ID" value="CAI48958"/>
        <property type="gene ID" value="NP_1734A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703085"/>
    <dbReference type="KEGG" id="nph:NP_1734A"/>
    <dbReference type="eggNOG" id="arCOG00081">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060704_1_0_2"/>
    <dbReference type="OrthoDB" id="94124at2157"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00120"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004590">
        <property type="term" value="F:orotidine-5'-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04725">
        <property type="entry name" value="OMP_decarboxylase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01215">
        <property type="entry name" value="OMPdecase_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018089">
        <property type="entry name" value="OMPdecase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011995">
        <property type="entry name" value="OMPdecase_type-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001754">
        <property type="entry name" value="OMPdeCOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02127">
        <property type="entry name" value="pyrF_sub2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43375">
        <property type="entry name" value="OROTIDINE 5'-PHOSPHATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43375:SF1">
        <property type="entry name" value="OROTIDINE 5'-PHOSPHATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00215">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00934">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00156">
        <property type="entry name" value="OMPDECASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0210">Decarboxylase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000066482" description="Orotidine 5'-phosphate decarboxylase">
        <location>
            <begin position="1"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01215"/>
        </source>
    </evidence>
    <sequence length="269" mass="29255" checksum="D72248AD21ED11AB" modified="2005-11-08" version="1">MSFFETLADRIDDRDSVVSVGLDPDPDRLPEFLDADLPRWAFNRRVIDATHEHAACYKPNAAFYEDSDGWRALEETIAYAHGKGVPVLLDAKRGDIGNTARQYATLLDDDGLGADAITANPYMGRDTLEPYLSRADKGVFILCRTSNSGGADFQNLTVGNDKRLYEYVAQRCQEWNEHENVGLVVGATAPEELESVRDLVDLPFLVPGVGAQGGDAAAAVDHGLADGVGLVNSSRGIIFAGEGAADEDEYFRAVGAAAKRLKQRLNKHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ISE6</accession>
    <name>Q3ISE6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 7">16S rRNA aminocarboxypropyltransferase</fullName>
            <ecNumber evidence="7">2.5.1.157</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">NP_1700A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Aminocarboxypropyltransferase that catalyzes the aminocarboxypropyl transfer on pseudouridine corresponding to position 914 in M.jannaschii 16S rRNA. It constitutes the last step in biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>an N(1)-methylpseudouridine in rRNA + S-adenosyl-L-methionine = N(1)-methyl-N(3)-[(3S)-3-amino-3-carboxypropyl]pseudouridine in rRNA + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:63296"/>
            <dbReference type="Rhea" id="RHEA-COMP:11634"/>
            <dbReference type="Rhea" id="RHEA-COMP:16310"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74890"/>
            <dbReference type="ChEBI" id="CHEBI:146234"/>
            <dbReference type="EC" id="2.5.1.157"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TDD superfamily. TSR3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.157" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322574.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ISE6"/>
    <dbReference type="STRING" id="348780.NP_1700A"/>
    <dbReference type="EnsemblBacteria" id="CAI48941">
        <property type="protein sequence ID" value="CAI48941"/>
        <property type="gene ID" value="NP_1700A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701229"/>
    <dbReference type="KEGG" id="nph:NP_1700A"/>
    <dbReference type="eggNOG" id="arCOG04733">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035060_4_1_2"/>
    <dbReference type="OrthoDB" id="7441at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106388">
        <property type="term" value="F:rRNA small subunit aminocarboxypropyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01116">
        <property type="entry name" value="TSR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007209">
        <property type="entry name" value="RNaseL-inhib-like_metal-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022968">
        <property type="entry name" value="Tsr3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007177">
        <property type="entry name" value="Tsr3_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002621">
        <property type="entry name" value="PRK02287.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20426:SF0">
        <property type="entry name" value="18S RRNA AMINOCARBOXYPROPYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20426">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN TSR3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04068">
        <property type="entry name" value="Fer4_RLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04034">
        <property type="entry name" value="Ribo_biogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 7" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RNase L inhibitor RLI-like possible metal-binding" evidence="9">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="16S/18S rRNA aminocarboxypropyltransferase Tsr3 C-terminal" evidence="8">
        <location>
            <begin position="40"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00014114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI48941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="167" mass="18464" checksum="820D2B5D4B1339F6" modified="2005-11-08" version="1">MELHVRYEGDDDPDKCSARKLARFDLADLHRSPRATPAGIVLDPHADRALSPTDADGGAATDRLIALDCSWETAEAEAFRLDGPHRSLPFLVAANPVNYGTPFQLTTVEAFAGALCILGHRDHAETVLSKFRWGHTFLELNDEPLRRYADCTDSTDVLDVQDDYLEA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IUG3</accession>
    <name>NIKR_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative nickel-responsive regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">NP_0252A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transcriptional regulator.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
        <text evidence="1">Binds 1 nickel ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the transcriptional regulatory CopG/NikR family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321856.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IUG3"/>
    <dbReference type="SMR" id="Q3IUG3"/>
    <dbReference type="STRING" id="348780.NP_0252A"/>
    <dbReference type="EnsemblBacteria" id="CAI48217">
        <property type="protein sequence ID" value="CAI48217"/>
        <property type="gene ID" value="NP_0252A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700803"/>
    <dbReference type="KEGG" id="nph:NP_0252A"/>
    <dbReference type="eggNOG" id="arCOG01008">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113319_0_0_2"/>
    <dbReference type="OrthoDB" id="9459at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010045">
        <property type="term" value="P:response to nickel cation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22231">
        <property type="entry name" value="RHH_NikR_HicB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1150">
        <property type="entry name" value="ACT-like. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="entry name" value="Met repressor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00476">
        <property type="entry name" value="NikR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027271">
        <property type="entry name" value="Acetolactate_synth/TF_NikR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002145">
        <property type="entry name" value="CopG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050192">
        <property type="entry name" value="CopG/NikR_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022988">
        <property type="entry name" value="Ni_resp_reg_NikR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014864">
        <property type="entry name" value="TF_NikR_Ni-bd_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002815">
        <property type="entry name" value="PRK02967.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34719">
        <property type="entry name" value="NICKEL-RESPONSIVE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34719:SF3">
        <property type="entry name" value="NICKEL-RESPONSIVE REGULATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08753">
        <property type="entry name" value="NikR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01402">
        <property type="entry name" value="RHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="Ribbon-helix-helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0533">Nickel</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_1000014077" description="Putative nickel-responsive regulator">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00476"/>
        </source>
    </evidence>
    <sequence length="141" mass="15992" checksum="656DDE7C9A95D22A" modified="2005-11-08" version="1">MTVVSVSMPESLLDRLDEFAEEHGYTGRSEVVREASRNLLGEFEDKKLEGRELMGVVTVVFDHETSSAEERMMQIRHDHEHTVVSNVHNHVGDHYCMELFILEGELDEISTFVGKVRATSDILSVDYSVVPLDDFEADLAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IUT5</accession>
    <name>Q3IUT5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ATP-grasp fold protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0008A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321734.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IUT5"/>
    <dbReference type="STRING" id="348780.NP_0008A"/>
    <dbReference type="EnsemblBacteria" id="CAI48095">
        <property type="protein sequence ID" value="CAI48095"/>
        <property type="gene ID" value="NP_0008A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700738"/>
    <dbReference type="KEGG" id="nph:NP_0008A"/>
    <dbReference type="eggNOG" id="arCOG01589">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_982143_0_0_2"/>
    <dbReference type="OrthoDB" id="312280at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013651">
        <property type="entry name" value="ATP-grasp_RimK-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21621:SF0">
        <property type="entry name" value="BETA-CITRYLGLUTAMATE SYNTHASE B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21621">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 MODIFICATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08443">
        <property type="entry name" value="RimK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="2">
        <location>
            <begin position="89"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="289" mass="31737" checksum="F11D9F5F1B0BD47B" modified="2005-11-08" version="1">MLRLAVATDAETLDRIRDPLADHGIEAEHVPTTERAQPLGEPFTETAFDVGFVYPSRLMEGGVADAALDIPWVNGRDAILRSRNKAGALTRLQRADVPVPESVYLSNPVDDATLRAAFEAFDPPVVIKPNSTTRGVGIAKAHDLDSFLGICEYLSLVHDYRAADDRSFLVQEYLPDARDYRAMVVDGECVGAVERRLPDEERRAGRWKHNVHRGATAEGVELPAEHRRLAVATADALDIDWLGVDLLVSGDRAVVNETNARPTVDAATKYEPGFYDQLAALIRRTAEQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETA0</accession>
    <name>A0A1U7ETA0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UspA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0036A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321748.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETA0"/>
    <dbReference type="STRING" id="348780.NP_0036A"/>
    <dbReference type="EnsemblBacteria" id="CAI48109">
        <property type="protein sequence ID" value="CAI48109"/>
        <property type="gene ID" value="NP_0036A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700744"/>
    <dbReference type="KEGG" id="nph:NP_0036A"/>
    <dbReference type="eggNOG" id="arCOG02053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_11_0_2"/>
    <dbReference type="OrthoDB" id="105697at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF6">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN UP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006276">
        <property type="entry name" value="UspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="143" mass="15570" checksum="58FFCEEC4D7E855E" modified="2017-05-10" version="1">MYDRILLPTDGSAGTNPAVDQAVSLAEETGAELHALFVVEDIPYAPEMMDDQVEATLRTIGEEAIENIRSRAAEADVELVTEIRDGTPHRSILDYADDAGCDLIVMGTHGRSGLDRYLLGSVTERIVRTADVPVLTVRKTDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETD3</accession>
    <name>A0A1U7ETD3_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">rpoB2</name>
        <name evidence="12" type="ordered locus">NP_0110A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321785.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETD3"/>
    <dbReference type="STRING" id="348780.NP_0110A"/>
    <dbReference type="EnsemblBacteria" id="CAI48146">
        <property type="protein sequence ID" value="CAI48146"/>
        <property type="gene ID" value="NP_0110A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703153"/>
    <dbReference type="KEGG" id="nph:NP_0110A"/>
    <dbReference type="eggNOG" id="arCOG01762">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000524_5_3_2"/>
    <dbReference type="OrthoDB" id="371768at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1100.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="entry name" value="RNA polymerase Rpb2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037034">
        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007175">
        <property type="entry name" value="PRK09606.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 12" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4 12" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RNA polymerase beta subunit protrusion" evidence="10">
        <location>
            <begin position="19"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="9">
        <location>
            <begin position="177"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="11">
        <location>
            <begin position="422"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI48146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="521" mass="59135" checksum="5CAF3426BE33FB24" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETF0</accession>
    <name>A0A1U7ETF0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Acyl-CoA synthetase</fullName>
            <ecNumber evidence="7">6.2.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">acs5</name>
        <name evidence="7" type="ordered locus">NP_0164A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.-" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321812.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETF0"/>
    <dbReference type="STRING" id="348780.NP_0164A"/>
    <dbReference type="EnsemblBacteria" id="CAI48173">
        <property type="protein sequence ID" value="CAI48173"/>
        <property type="gene ID" value="NP_0164A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702488"/>
    <dbReference type="KEGG" id="nph:NP_0164A"/>
    <dbReference type="eggNOG" id="arCOG04201">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_10_2"/>
    <dbReference type="OrthoDB" id="193284at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016405">
        <property type="term" value="F:CoA-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015645">
        <property type="term" value="F:fatty acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004321">
        <property type="term" value="F:fatty-acyl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018486">
        <property type="entry name" value="Hemopexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051087">
        <property type="entry name" value="Mitochondrial_ACSM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43605:SF10">
        <property type="entry name" value="ACYL-COA SYNTHETASE MEDIUM CHAIN FAMILY MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43605">
        <property type="entry name" value="ACYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00024">
        <property type="entry name" value="HEMOPEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="5">
        <location>
            <begin position="38"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="6">
        <location>
            <begin position="456"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="544" mass="59480" checksum="B5FAFE6D6AE2E41C" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETF9</accession>
    <name>A0A1U7ETF9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Nitroreductase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_0176A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the nitroreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321818.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETF9"/>
    <dbReference type="STRING" id="348780.NP_0176A"/>
    <dbReference type="EnsemblBacteria" id="CAI48179">
        <property type="protein sequence ID" value="CAI48179"/>
        <property type="gene ID" value="NP_0176A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700780"/>
    <dbReference type="KEGG" id="nph:NP_0176A"/>
    <dbReference type="eggNOG" id="arCOG00288">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070764_4_5_2"/>
    <dbReference type="OrthoDB" id="287850at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02137">
        <property type="entry name" value="MhqN-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="entry name" value="NADH Oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43673">
        <property type="entry name" value="NAD(P)H NITROREDUCTASE YDGI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43673:SF10">
        <property type="entry name" value="NADH DEHYDROGENASE_NAD(P)H NITROREDUCTASE XCC3605-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="FMN-dependent nitroreductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="4">
        <location>
            <begin position="7"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="189"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="191"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="213"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI48179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="223" mass="25140" checksum="A304926543BC3719" modified="2017-05-10" version="1">MQFDELLRARRSVHQYSDESIDEATLETIFEAATLAPSGYNLQPWEFLALRDPERKQALREIAYDQEHVTEADTAVVVLGHTDPTEHAEPVFDDWLAKGYVPNEDVRDALLENVEGMAEMPEDERRIWTTRSTALAAMTLMYAAWDEGVASCPMEGFDAEALVEEFDIPDAYEPVMLITLGYPAADAADVENERKGRRPVDDVVHYDEFEPAETTSLAVPNKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETS1</accession>
    <name>A0A1U7ETS1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0454A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321954.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETS1"/>
    <dbReference type="STRING" id="348780.NP_0454A"/>
    <dbReference type="EnsemblBacteria" id="CAI48318">
        <property type="protein sequence ID" value="CAI48318"/>
        <property type="gene ID" value="NP_0454A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700860"/>
    <dbReference type="KEGG" id="nph:NP_0454A"/>
    <dbReference type="eggNOG" id="arCOG14874">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1718265_0_0_2"/>
    <dbReference type="OrthoDB" id="260878at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="152" mass="16888" checksum="156CC1C2392C086A" modified="2017-05-10" version="1">MGSHADRVADALREALREWRPDREWTTERYVGRTPVDVAGEGEPLALVELEWRRADPANNTVKLFRRLDADATERPAVVVQLFTGYYALADGGVSSKQRNAVFAGERLAQTVDAVDYEPLRLGIDPPKRGGTLPDGWRDAVEEAAAEVDDRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU19</accession>
    <name>A0A1U7EU19_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF5786 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0700A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322077.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU19"/>
    <dbReference type="EnsemblBacteria" id="CAI48441">
        <property type="protein sequence ID" value="CAI48441"/>
        <property type="gene ID" value="NP_0700A"/>
    </dbReference>
    <dbReference type="GeneID" id="54763306"/>
    <dbReference type="KEGG" id="nph:NP_0700A"/>
    <dbReference type="eggNOG" id="arCOG06346">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_211275_0_0_2"/>
    <dbReference type="OrthoDB" id="286231at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043902">
        <property type="entry name" value="DUF5786"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19099">
        <property type="entry name" value="DUF5786"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5786" evidence="2">
        <location>
            <begin position="3"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="55" mass="6394" checksum="F7380AD01CA2F700" modified="2017-05-10" version="1">MSMGAYDEDEHERREKKAATVDAAFDDERTNYEGEIEYEAGDSAEELLDQFKQMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU53</accession>
    <name>A0A1U7EU53_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PrsW family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0754A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48468.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU53"/>
    <dbReference type="STRING" id="348780.NP_0754A"/>
    <dbReference type="EnsemblBacteria" id="CAI48468">
        <property type="protein sequence ID" value="CAI48468"/>
        <property type="gene ID" value="NP_0754A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_0754A"/>
    <dbReference type="eggNOG" id="arCOG02985">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_820411_0_0_2"/>
    <dbReference type="OrthoDB" id="248468at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026898">
        <property type="entry name" value="PrsW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36844">
        <property type="entry name" value="PROTEASE PRSW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36844:SF1">
        <property type="entry name" value="PROTEASE PRSW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13367">
        <property type="entry name" value="PrsW-protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48468.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="352" mass="38114" checksum="B62F15BEE8151710" modified="2017-05-10" version="1">MKDPVEARADTDEDLYDVADWEPRTALDRLAIRTYQGAIRASRRLLVFLGFVVLFVIIALSGAGGLILEDPFVLGLAALSVVPAALLAAYIYRADITTREPLSLLLATFILAVLFAGFAAIINTAFIFLQLLPFFGLVLFFYLVVGPVEESVKLLAVWLYPYRDARFDSVVDGAVYGAVAGLGFAAIENVLYITQNLQTPAMVLATASANLLADFTETLAAGGEITAIRGLAGPGHVIYSAFAGYYLGLAKFNRENAGPIVAKGLLIAAFIHATYNTLVGIIPGVASLLVPGVPVLVLFFGFVVIYQGLFLYLLYRKLKRYEGAYRRGHDGQHRAVAFESELTEFDPDSGQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUB2</accession>
    <name>A0A1U7EUB2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0894A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322174.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUB2"/>
    <dbReference type="STRING" id="348780.NP_0894A"/>
    <dbReference type="EnsemblBacteria" id="CAI48538">
        <property type="protein sequence ID" value="CAI48538"/>
        <property type="gene ID" value="NP_0894A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700991"/>
    <dbReference type="KEGG" id="nph:NP_0894A"/>
    <dbReference type="HOGENOM" id="CLU_1965628_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="127" mass="13871" checksum="10F71E36791B35FD" modified="2017-05-10" version="1">MRRRTFLSTVAAGTIAASAGCSSEELGLGLDLDSPTGVIEAFLDAVDDEEEPADQYVHPDTDFEVDDSRAADAVADTAVTETIEPVEQDDDVSVVAVEFLGDHHEDVQFELRRDGNAWRLWDETTVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUI8</accession>
    <name>A0A1U7EUI8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">PQQ repeat protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_1104A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322279.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUI8"/>
    <dbReference type="STRING" id="348780.NP_1104A"/>
    <dbReference type="EnsemblBacteria" id="CAI48643">
        <property type="protein sequence ID" value="CAI48643"/>
        <property type="gene ID" value="NP_1104A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701053"/>
    <dbReference type="KEGG" id="nph:NP_1104A"/>
    <dbReference type="eggNOG" id="arCOG02490">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047517_0_0_2"/>
    <dbReference type="OrthoDB" id="8638at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002372">
        <property type="entry name" value="PQQ_rpt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34512">
        <property type="entry name" value="CELL SURFACE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34512:SF30">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN ASSEMBLY FACTOR BAMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13360">
        <property type="entry name" value="PQQ_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyrrolo-quinoline quinone repeat" evidence="2">
        <location>
            <begin position="31"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrrolo-quinoline quinone repeat" evidence="2">
        <location>
            <begin position="148"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="402" mass="42896" checksum="EB0A9200C198918D" modified="2017-05-10" version="1">MSSDLTLSATAALGDIDPCGSRQAGRRSGVTLDGDGPVVGRADGTVAAFTHDGTERWTVDGTGSAVTLVSTGDGVLVGERSERGRIQCRSRNGKRRWQHDAADDVGGPTKDTRFFLPMVVDAAVGSDGTCFVAARRYERRNGGRGFESSVYAFARDGTLRWRYDADASPIGVAPLDEGDGVAVAYNRCPGGHDSGLVVLDDNGSERWTWDPKPGADRRVGDVAVADGSVVVTSHADYRGYRLTDGSVEWAVDLGRPQSDGGVYTYPNHVHATASGAVFLTGNTFPQEGRETDERHENEQTAFGYTPDGKRRWQAAVGGFAHEVGTDGERMLVPTAQHFRDCDPTVHGWTLFDVADGPVAAASTDGVVTAAAVDGERGAVVEEPVRYHVDETLRGEYSLRMLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUM0</accession>
    <name>A0A1U7EUM0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">nicotinate phosphoribosyltransferase</fullName>
            <ecNumber evidence="2">6.3.4.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">pncB</name>
        <name evidence="10" type="ordered locus">NP_1174A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>5-phospho-alpha-D-ribose 1-diphosphate + nicotinate + ATP + H2O = nicotinate beta-D-ribonucleotide + ADP + phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:36163"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32544"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from nicotinate: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.21" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322314.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUM0"/>
    <dbReference type="STRING" id="348780.NP_1174A"/>
    <dbReference type="EnsemblBacteria" id="CAI48678">
        <property type="protein sequence ID" value="CAI48678"/>
        <property type="gene ID" value="NP_1174A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703039"/>
    <dbReference type="KEGG" id="nph:NP_1174A"/>
    <dbReference type="eggNOG" id="arCOG01481">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043773_0_0_2"/>
    <dbReference type="OrthoDB" id="371831at2157"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00457"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004516">
        <property type="term" value="F:nicotinate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004514">
        <property type="term" value="F:nicotinate-nucleotide diphosphorylase (carboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01571">
        <property type="entry name" value="NAPRTase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.20">
        <property type="entry name" value="Quinolinate phosphoribosyl transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053190">
        <property type="entry name" value="NAPRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035809">
        <property type="entry name" value="NAPRTase_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007229">
        <property type="entry name" value="Nic_PRibTrfase-Fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037128">
        <property type="entry name" value="Quinolinate_PRibosylTase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002638">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022412">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006415">
        <property type="entry name" value="PRK08662.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43202:SF1">
        <property type="entry name" value="NICOTINATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43202">
        <property type="entry name" value="NICOTINATE-NUCLEOTIDE PYROPHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01729">
        <property type="entry name" value="QRPTase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02749">
        <property type="entry name" value="QRPTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000484">
        <property type="entry name" value="NAPRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54675">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Quinolinate phosphoribosyl transferase N-terminal" evidence="9">
        <location>
            <begin position="17"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Quinolinate phosphoribosyl transferase C-terminal" evidence="8">
        <location>
            <begin position="112"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI48678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="378" mass="40288" checksum="A720A56B3D792613" modified="2017-05-10" version="1">MFDTVPGSAIESGRATDAYFLRTEEALEHAGRNPHVVAEVTADQFPTGEFELLAGLEDAARLLEGRDIDVDALPEGTLFDGGPVMRIEGPYLEFARLETSLLGFLSHPTGMATAALEARQAAPDTSLLSFGARHVHPSIAGTVDRSALIAGFDGFSHVAAGDILDREASGTMPHALMICFGPGNQEEAWQAFDEAVAPDVPRIALCDTYDDEVDEAVRAAELGFDSVRLDTTGSRRGDFRHIVRETRWKLDVAGHEDVDIFVSGGITPEAIRNLKDVADGFGVGSHITNADPVDFALDIVEVEGDPAAKRGKLSGTKAVYRTTDGGHHIALADADPPDDADPLLEPLIRDGDIVADVELSAAAERARADAERVGFGEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUM6</accession>
    <name>A0A1U7EUM6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DUF4147 domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_1162A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUM6"/>
    <dbReference type="STRING" id="348780.NP_1162A"/>
    <dbReference type="EnsemblBacteria" id="CAI48672">
        <property type="protein sequence ID" value="CAI48672"/>
        <property type="gene ID" value="NP_1162A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_1162A"/>
    <dbReference type="eggNOG" id="arCOG04170">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032279_1_1_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008887">
        <property type="term" value="F:glycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10180">
        <property type="entry name" value="Glycerate kinase, MOFRL-like N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1480.10">
        <property type="entry name" value="MOFRL domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037035">
        <property type="entry name" value="GK-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038614">
        <property type="entry name" value="GK_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007835">
        <property type="entry name" value="MOFRL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025286">
        <property type="entry name" value="MOFRL_assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039760">
        <property type="entry name" value="MOFRL_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12227">
        <property type="entry name" value="GLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12227:SF0">
        <property type="entry name" value="GLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13660">
        <property type="entry name" value="DUF4147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05161">
        <property type="entry name" value="MOFRL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82544">
        <property type="entry name" value="GckA/TtuD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MOFRL-associated" evidence="2">
        <location>
            <begin position="15"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="MOFRL" evidence="1">
        <location>
            <begin position="322"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="426" mass="42562" checksum="C2E63791C5A8B55E" modified="2017-05-10" version="1">MFDPLSTPSPAHELAVECLTAGVRAAQPKRAVERHCDIEGSTLRIGDGSYDLDAYDSLRILGGGKAADGLAAALEGLLGERLDGGIVVTGHRTAAPDRVTVREGNHPSPGPDSVDGATALLERAEAADGQTLVLAPMTGGGSALLCAPADGLSAADIRTVTEALLDAGASVDELNAVRRVCSEIKGGGLAAAAAPATVVGVVMSDVVGDDPAVVASGPTVPVEAAPDVAATVLDRYGVDAPAVRRWLSSATPESPSVAARNHVIASGWDAVDAARAHAAAAGYQTCVLSTHLEGEAEQSGRFHAAVATDVALRDVPVEPPAVILSGGETTVSVSGDGVGGPNMEFALAAAPKLPDEAVVGAVDTDGSDGSTDAAGALVDADTVDPQVARTALDENDSYRALADAGALLFSGSTGTNVNDLRVVVVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUN2</accession>
    <name>A0A1U7EUN2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chromosome partition protein Smc</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 9" type="primary">smc</name>
        <name evidence="9" type="ordered locus">NP_1216A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for chromosome condensation and partitioning.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="6">Contains large globular domains required for ATP hydrolysis at each terminus and a third globular domain forming a flexible hinge near the middle of the molecule. These domains are separated by coiled-coil structures.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SMC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322335.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUN2"/>
    <dbReference type="STRING" id="348780.NP_1216A"/>
    <dbReference type="EnsemblBacteria" id="CAI48699">
        <property type="protein sequence ID" value="CAI48699"/>
        <property type="gene ID" value="NP_1216A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703209"/>
    <dbReference type="KEGG" id="nph:NP_1216A"/>
    <dbReference type="eggNOG" id="arCOG00371">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001042_2_2_2"/>
    <dbReference type="OrthoDB" id="9143at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1060.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01894">
        <property type="entry name" value="Smc_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011890">
        <property type="entry name" value="SMC_prok"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02169">
        <property type="entry name" value="SMC_prok_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02168">
        <property type="entry name" value="SMC_prok_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43977">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="Smc hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="8">
        <location>
            <begin position="544"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="397"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="921"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="993"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="397"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="410"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="938"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="32"/>
            <end position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI48699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="1192" mass="134095" checksum="050E60C9FF37DA82" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUN8</accession>
    <name>A0A1U7EUN8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">IS1341-type transposase ISNph10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">NP_1210A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322332.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUN8"/>
    <dbReference type="STRING" id="348780.NP_1210A"/>
    <dbReference type="EnsemblBacteria" id="CAI48696">
        <property type="protein sequence ID" value="CAI48696"/>
        <property type="gene ID" value="NP_1210A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701084"/>
    <dbReference type="KEGG" id="nph:NP_1210A"/>
    <dbReference type="eggNOG" id="arCOG00684">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_0_1_2"/>
    <dbReference type="OrthoDB" id="33505at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021027">
        <property type="entry name" value="Transposase_put_HTH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF25">
        <property type="entry name" value="RNA-GUIDED DNA ENDONUCLEASE INSQ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12323">
        <property type="entry name" value="HTH_OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Transposase putative helix-turn-helix" evidence="11">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="9">
        <location>
            <begin position="187"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="10">
        <location>
            <begin position="303"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="215"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI48696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="413" mass="48003" checksum="A12C9EDBA018D100" modified="2017-05-10" version="1">MEHSHRYHAYPTQEVAERLERHLDVHRQLYNHIRWEYEQAPEDDKPSEYDQNNKLPGWKRKWPVFSELHSKAAQATVARFHRNLSNLRKKKEKGYNVGRLKRQAPTDYRSVTYNQSGFDLDEKRGHDRYAHVRFSKIGWVKIRYHRPIPDHATIKEVTFKKETTGEWFVSFGLETDDTDLPEKTDVESLNASNSVGIDLGILNYIHTSDGKTVDWLDLEDEYERLRREQRKLSRKERGSSNYEKQRLKVAKVKRHIKRKVLDYQHKITTWLVKEYAAVFVEELNVKGMLEQSHNARNKQDAAWRQFITLLEYKADLYGCHVVQVEARGTTKECASCGVETAKPIWVRKHSCPSCGFETDRDANAAMNVLQRGFSELGLGWPEDTPVETVTATDTPEFQCVSASHVVETGSLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUP3</accession>
    <name>A0A1U7EUP3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Assimilatory nitrate reductase</fullName>
            <ecNumber evidence="8">1.7.7.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">nasA2</name>
        <name evidence="8" type="synonym">narB2</name>
        <name evidence="8" type="ordered locus">NP_1244A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.7.7.2" evidence="8"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUP3"/>
    <dbReference type="STRING" id="348780.NP_1244A"/>
    <dbReference type="EnsemblBacteria" id="CAI48713">
        <property type="protein sequence ID" value="CAI48713"/>
        <property type="gene ID" value="NP_1244A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_1244A"/>
    <dbReference type="eggNOG" id="arCOG01491">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_13_4_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047889">
        <property type="term" value="F:ferredoxin-nitrate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.90">
        <property type="entry name" value="ADC-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.228.10">
        <property type="entry name" value="Dimethylsulfoxide Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006655">
        <property type="entry name" value="Mopterin_OxRdtase_prok_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054894">
        <property type="entry name" value="Nitr_red_NasA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050123">
        <property type="entry name" value="Prok_molybdopt-oxidoreductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041323">
        <property type="entry name" value="Nitr_red_NasA_Halo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43105:SF9">
        <property type="entry name" value="NADPH-FE(3+) OXIDOREDUCTASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43105">
        <property type="entry name" value="RESPIRATORY NITRATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04879">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00926">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53706">
        <property type="entry name" value="Formate dehydrogenase/DMSO reductase, domains 1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00490">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="7">
        <location>
            <begin position="2"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="584"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="653"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="672"/>
            <end position="689"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI48713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="695" mass="73374" checksum="84D1B70DE08637C3" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUS9</accession>
    <name>A0A1U7EUS9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Rpa-associated protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpap3</name>
        <name evidence="1" type="ordered locus">NP_1280A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322366.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUS9"/>
    <dbReference type="STRING" id="348780.NP_1280A"/>
    <dbReference type="EnsemblBacteria" id="CAI48731">
        <property type="protein sequence ID" value="CAI48731"/>
        <property type="gene ID" value="NP_1280A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701098"/>
    <dbReference type="KEGG" id="nph:NP_1280A"/>
    <dbReference type="eggNOG" id="arCOG02257">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080075_1_0_2"/>
    <dbReference type="OrthoDB" id="56523at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="196" mass="21856" checksum="3C2BD2F0462F390A" modified="2017-05-10" version="1">MSDSEPSREVARRAFAAEFNDATYVFKESDDERAPVYSLLPTGERANRVFIVGTLTETEDIGEDSEYWRGRIVDPTGTFFTYAGQYQPEATSVLREAETPAYVAVVGKPRTYETDSGEVNVSLRPESITVVDGATRDRWVAETAERTVERIEAFDSAHEYAAMAETQYDPDLSVYRDQAIAALEDLETDAEQPAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUT1</accession>
    <name>A0A1U7EUT1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">PtpS family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_1342A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322397.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUT1"/>
    <dbReference type="STRING" id="348780.NP_1342A"/>
    <dbReference type="EnsemblBacteria" id="CAI48762">
        <property type="protein sequence ID" value="CAI48762"/>
        <property type="gene ID" value="NP_1342A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701120"/>
    <dbReference type="KEGG" id="nph:NP_1342A"/>
    <dbReference type="eggNOG" id="arCOG02172">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111016_6_3_2"/>
    <dbReference type="OrthoDB" id="6529at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="6-pyruvoyl tetrahydropterin synthase/QueD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007115">
        <property type="entry name" value="6-PTP_synth/QueD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038418">
        <property type="entry name" value="6-PTP_synth/QueD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12589:SF7">
        <property type="entry name" value="6-PYRUVOYL TETRAHYDROBIOPTERIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12589">
        <property type="entry name" value="PYRUVOYL TETRAHYDROBIOPTERIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01242">
        <property type="entry name" value="PTPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI48762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="123" mass="13160" checksum="31F75F4EFEDD0216" modified="2017-05-10" version="1">MFTVTVTETFVAQHFLTLPDPPADEATLHSHTFEAEVTFRGPELGPHGYLLDIDAAREALSAAAETYRDETLNDHLDGNPSVERLAVALFADLSSLEAPAVTELTVAISEDDTAVASYTAAVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUT7</accession>
    <name>A0A1U7EUT7_NATPD</name>
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        <submittedName>
            <fullName evidence="3">CobC/GpmA family phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_1332A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.3.-" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322392.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUT7"/>
    <dbReference type="STRING" id="348780.NP_1332A"/>
    <dbReference type="EnsemblBacteria" id="CAI48757">
        <property type="protein sequence ID" value="CAI48757"/>
        <property type="gene ID" value="NP_1332A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702769"/>
    <dbReference type="KEGG" id="nph:NP_1332A"/>
    <dbReference type="eggNOG" id="arCOG01991">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033323_9_5_2"/>
    <dbReference type="OrthoDB" id="304253at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050275">
        <property type="entry name" value="PGM_Phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100">
        <property type="entry name" value="BROAD-SPECIFICITY PHOSPHATASE YOR283W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100:SF62">
        <property type="entry name" value="GLUCOSYL-3-PHOSPHOGLYCERATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000709">
        <property type="entry name" value="6PFK_2-Ptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="1">
        <location>
            <position position="9"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="8"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="204" mass="22944" checksum="E45D462C37B657CD" modified="2017-05-10" version="1">MTRIVAVRHGETDWNRNGRMQGWAPVPLNETGHEQAAAAASWLSDTYDIDRVIASDLHRTEQTAERILDATEPADVRFDPGWRERDLGVYQGLTYQDIESRFPEFGLGETAYEATLALPEGGESLRDMADRVTGQFETVRDRYAGETVLVVTHGGPLHVLLGYARDLSLPAALKNHHLDNCAVTEFRVAGREVEVVRENDTRWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUW1</accession>
    <name>A0A1U7EUW1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1390A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322421.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUW1"/>
    <dbReference type="EnsemblBacteria" id="CAI48786">
        <property type="protein sequence ID" value="CAI48786"/>
        <property type="gene ID" value="NP_1390A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701135"/>
    <dbReference type="KEGG" id="nph:NP_1390A"/>
    <dbReference type="eggNOG" id="arCOG04587">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1264542_0_0_2"/>
    <dbReference type="OrthoDB" id="212703at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055534">
        <property type="entry name" value="DUF7110"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23422">
        <property type="entry name" value="DUF7110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="210" mass="23590" checksum="4FBDFCBB13F07A4A" modified="2017-05-10" version="1">MTSRVYRLHSTLELQLEDVHEFFDGYDLPVEIEDVDVTRRNNTLIVSAIAAEDNISKYTPTAQLKASVTENRLYETEDGWQETPPEDHESGSTGSDDGPQWGNFSDGAQVQEEEVEVNSKLVEYACFKGDRETVLQNTALQYPMFEVLCDLAKYADSGTLTAIATVDGELEAVRIVDGEQREASIEVVEDPRERDSENAVNWRDNEFISG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUW5</accession>
    <name>A0A1U7EUW5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ACT domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1404A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322428.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUW5"/>
    <dbReference type="STRING" id="348780.NP_1404A"/>
    <dbReference type="EnsemblBacteria" id="CAI48793">
        <property type="protein sequence ID" value="CAI48793"/>
        <property type="gene ID" value="NP_1404A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701139"/>
    <dbReference type="KEGG" id="nph:NP_1404A"/>
    <dbReference type="eggNOG" id="arCOG02316">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100860_0_0_2"/>
    <dbReference type="OrthoDB" id="30884at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014424">
        <property type="entry name" value="UCP004897_ACT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004897">
        <property type="entry name" value="UCP004897_ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="1">
        <location>
            <begin position="97"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="167" mass="18507" checksum="8821E53FB840940D" modified="2017-05-10" version="1">MFDEIMRKFEDSPSQQAVIRLLLERGFSVNDDGRVVSGGIEIPNTGIAREIDVDRRVVDATTDAILDDDELRRIFQNISAIPSLMDLAPVLDLTVLTVRVGDADQPGIVAAVTSLLADNGVSIRQTISEDPEFTDEPKLYIVTDEQLDGELINEVRALPFVRKIELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUX3</accession>
    <name>A0A1U7EUX3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Aldehyde ferredoxin oxidoreductase</fullName>
            <ecNumber evidence="10">1.2.7.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">aor2</name>
        <name evidence="10" type="ordered locus">NP_1432A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>tungstopterin</name>
            <dbReference type="ChEBI" id="CHEBI:30402"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AOR/FOR family.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.5" evidence="10"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322442.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUX3"/>
    <dbReference type="STRING" id="348780.NP_1432A"/>
    <dbReference type="EnsemblBacteria" id="CAI48807">
        <property type="protein sequence ID" value="CAI48807"/>
        <property type="gene ID" value="NP_1432A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702508"/>
    <dbReference type="KEGG" id="nph:NP_1432A"/>
    <dbReference type="eggNOG" id="arCOG00706">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020364_1_0_2"/>
    <dbReference type="OrthoDB" id="30771at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033726">
        <property type="term" value="F:aldehyde ferredoxin oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.569.10">
        <property type="entry name" value="Aldehyde Ferredoxin Oxidoreductase Protein, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.599.10">
        <property type="entry name" value="Aldehyde Ferredoxin Oxidoreductase Protein, subunit A, domain 3"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.60.9.10">
        <property type="entry name" value="Aldehyde ferredoxin oxidoreductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ald_Fedxn_OxRdtase_dom2"/>
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        <property type="entry name" value="Ald_Fedxn_OxRdtase_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013983">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036503">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001203">
        <property type="entry name" value="OxRdtase_Ald_Fedxn_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036021">
        <property type="entry name" value="Tungsten_al_ferr_oxy-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051919">
        <property type="entry name" value="W-dependent_AOR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30038">
        <property type="entry name" value="ALDEHYDE FERREDOXIN OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30038:SF7">
        <property type="entry name" value="TUNGSTEN-CONTAINING GLYCERALDEHYDE-3-PHOSPHATE:FERREDOXIN OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01314">
        <property type="entry name" value="AFOR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02730">
        <property type="entry name" value="AFOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00790">
        <property type="entry name" value="AFOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48310">
        <property type="entry name" value="Aldehyde ferredoxin oxidoreductase, C-terminal domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Aldehyde ferredoxin oxidoreductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde ferredoxin oxidoreductase N-terminal" evidence="9">
        <location>
            <begin position="6"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI48807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="563" mass="60538" checksum="8B4216595C65B50A" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV64</accession>
    <name>A0A1U7EV64_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1602A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV64"/>
    <dbReference type="STRING" id="348780.NP_1602A"/>
    <dbReference type="EnsemblBacteria" id="CAI48892">
        <property type="protein sequence ID" value="CAI48892"/>
        <property type="gene ID" value="NP_1602A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_1602A"/>
    <dbReference type="HOGENOM" id="CLU_1943918_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="129" mass="13334" checksum="732BBEF6BE1AD7FB" modified="2017-05-10" version="1">MPPHTPRHDRQTLLATVGSVAAVAAAGCLATGDESDTGGESGGSPAGELGSPAAETGRHDVTASHEDIRSFIYQPPDPDDQSPGGPSPAGPKRARTVQRTTPARSRSGTDTNARHQCHFHITGTTDEPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV95</accession>
    <name>A0A1U7EV95_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Succinate-semialdehyde dehydrogenase</fullName>
            <ecNumber evidence="6">1.2.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">gabD</name>
        <name evidence="6" type="synonym">aldH1</name>
        <name evidence="6" type="ordered locus">NP_1686A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322567.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV95"/>
    <dbReference type="STRING" id="348780.NP_1686A"/>
    <dbReference type="EnsemblBacteria" id="CAI48934">
        <property type="protein sequence ID" value="CAI48934"/>
        <property type="gene ID" value="NP_1686A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702500"/>
    <dbReference type="KEGG" id="nph:NP_1686A"/>
    <dbReference type="eggNOG" id="arCOG01252">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_1_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006081">
        <property type="term" value="P:aldehyde metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07101">
        <property type="entry name" value="ALDH_SSADH2_GabD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000009">
        <property type="entry name" value="Aldehyde dehydrogenase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012394">
        <property type="entry name" value="Aldehyde_DH_NAD(P)"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006916">
        <property type="entry name" value="PRK09407.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036492">
        <property type="entry name" value="ALDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="5">
        <location>
            <begin position="30"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI48934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="525" mass="56315" checksum="1A1BA8685F3155AE" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVM9</accession>
    <name>A0A1U7EVM9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC-type transport system ATP-binding protein (Probable substrate urea/short-chain amides)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">urtD</name>
        <name evidence="5" type="synonym">abc10a2</name>
        <name evidence="5" type="ordered locus">NP_1998A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322719.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVM9"/>
    <dbReference type="STRING" id="348780.NP_1998A"/>
    <dbReference type="EnsemblBacteria" id="CAI49090">
        <property type="protein sequence ID" value="CAI49090"/>
        <property type="gene ID" value="NP_1998A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702398"/>
    <dbReference type="KEGG" id="nph:NP_1998A"/>
    <dbReference type="eggNOG" id="arCOG00927">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OrthoDB" id="44250at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03219">
        <property type="entry name" value="ABC_Mj1267_LivG_branched"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051120">
        <property type="entry name" value="ABC_AA/LPS_Transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45772">
        <property type="entry name" value="CONSERVED COMPONENT OF ABC TRANSPORTER FOR NATURAL AMINO ACIDS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45772:SF8">
        <property type="entry name" value="HIGH-AFFINITY BRANCHED-CHAIN AMINO ACID TRANSPORT ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="26"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="258" mass="28122" checksum="F94710473E23B4CD" modified="2017-05-10" version="1">MSTPDDPATRLQPAVKATGLDRDAILATERLTKNFGGLTAVDEVDFTVDEGELRCLIGPNGAGKSTLLELITGQLTPTEGRIYFDGMDLTQLPPHERIDAGLSVKFQSPHVYEDLTVAQNLQVPLQRVDSGDLEATTYETLDEIGLADRAETPAGDLSHGQQQRLEIGMATTLEPKLMLLDEPVAGMSVEETASVADLIRSLNDEGMAFVVIEHDMEFVREISDQVTVLNQGSIFRQGPIDDIESDPEVKRIYLGEDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVQ4</accession>
    <name>A0A1U7EVQ4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dihydropyrimidine dehydrogenase</fullName>
            <ecNumber evidence="2">1.3.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">dpd</name>
        <name evidence="2" type="ordered locus">NP_2026A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.3.1.2" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322733.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVQ4"/>
    <dbReference type="STRING" id="348780.NP_2026A"/>
    <dbReference type="EnsemblBacteria" id="CAI49104">
        <property type="protein sequence ID" value="CAI49104"/>
        <property type="gene ID" value="NP_2026A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701330"/>
    <dbReference type="KEGG" id="nph:NP_2026A"/>
    <dbReference type="eggNOG" id="arCOG00605">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1118194_0_0_2"/>
    <dbReference type="OrthoDB" id="145053at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082:SF36">
        <property type="entry name" value="DUS-LIKE FMN-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082">
        <property type="entry name" value="TRNA-DIHYDROURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUS-like FMN-binding" evidence="1">
        <location>
            <begin position="85"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="255" mass="26539" checksum="0BA0D2B80DD7A8BF" modified="2017-05-10" version="1">MFAPRLAVASLSGESDADWAAAAAPHVGCAFLGGLAIDAETRRAAAALAERDRTEFLPDDPIAFIDKQLAALAGTPVRPAFNVRSATVDPIVEAAAVCADRDAIVELNAHCRQSEMCAAGAGESLLKDDDRLCEQVRAAADTGATVSVKVRTEVDGVDLPALAAALSAAGASVLHVDAMDSEHVVGAVTEAAPNAFVVANNGVRDRKTTREYLDYGADAVSVGRPSDDPALLARVRRAVDEWFDSQRQTEVSADG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVS4</accession>
    <name>A0A1U7EVS4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cupin 2 barrel domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2080A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322759.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVS4"/>
    <dbReference type="STRING" id="348780.NP_2080A"/>
    <dbReference type="EnsemblBacteria" id="CAI49131">
        <property type="protein sequence ID" value="CAI49131"/>
        <property type="gene ID" value="NP_2080A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701346"/>
    <dbReference type="KEGG" id="nph:NP_2080A"/>
    <dbReference type="eggNOG" id="arCOG03003">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103066_3_2_2"/>
    <dbReference type="OrthoDB" id="241629at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053146">
        <property type="entry name" value="QDO-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36440">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_8G07350)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36440:SF1">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_8G07350)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin type-2" evidence="1">
        <location>
            <begin position="42"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="164" mass="17248" checksum="6C67063289034650" modified="2017-05-10" version="1">MGEFDTPTTGEATEAAAFEFLDTLSYVRIDGDATDGQRAVVEMRLREGHAPPMHIHREATETIHVLDGEIDVHTPDSVTSLDTGASAVLPNGEPHSLHAVTQATILASTSPAGFEQFIEAVGKPAATETVPTTPPSEDAIGRVSELAPAHDIEIVGPPPVEPPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVS8</accession>
    <name>A0A1U7EVS8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">LppX domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2126A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322782.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVS8"/>
    <dbReference type="STRING" id="348780.NP_2126A"/>
    <dbReference type="EnsemblBacteria" id="CAI49154">
        <property type="protein sequence ID" value="CAI49154"/>
        <property type="gene ID" value="NP_2126A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701358"/>
    <dbReference type="KEGG" id="nph:NP_2126A"/>
    <dbReference type="eggNOG" id="arCOG02470">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1044335_0_0_2"/>
    <dbReference type="OrthoDB" id="137725at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.50.20.10">
        <property type="entry name" value="Lipoprotein localisation LolA/LolB/LppX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029046">
        <property type="entry name" value="LolA/LolB/LppX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052944">
        <property type="entry name" value="Sporulation_related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37507">
        <property type="entry name" value="SPORULATION PROTEIN YDCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37507:SF2">
        <property type="entry name" value="SPORULATION PROTEIN YDCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89392">
        <property type="entry name" value="Prokaryotic lipoproteins and lipoprotein localization factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="266" mass="29445" checksum="2FA34CFF20C95D48" modified="2017-05-10" version="1">MATVRELLVVGLLAGALLAGASVLESPTGDAENEAPDPDTLWEATFVYSEALESIEGERTTIVETGNETRTRTVAVAERPYTDYRTAVLEAPLPGDEGEVYVSNATVNWWYNPTTNSADYFRPAEPFTDAEIRDARSENAEERRALVEVEYAGTETIADREAHVLDVAAANETATRGIELLVGDREFVFALDSVDPNDELLVVETRVWIDAEYDYPLKEQAVFIEDDDERHVYTERFESVTFNGDIGDDAFAFEPPEDAMVTDISG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVU9</accession>
    <name>A0A1U7EVU9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2188A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322813.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVU9"/>
    <dbReference type="STRING" id="348780.NP_2188A"/>
    <dbReference type="EnsemblBacteria" id="CAI49185">
        <property type="protein sequence ID" value="CAI49185"/>
        <property type="gene ID" value="NP_2188A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701374"/>
    <dbReference type="KEGG" id="nph:NP_2188A"/>
    <dbReference type="eggNOG" id="arCOG04569">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110566_0_0_2"/>
    <dbReference type="OrthoDB" id="204348at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043821">
        <property type="entry name" value="DUF5799"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19113">
        <property type="entry name" value="DUF5799"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="147" mass="15861" checksum="BB41AEFA493BEEB4" modified="2017-05-10" version="1">MTDWQDMVVGDRMAVDSEFASRVDDSQFTRQEWGLIMTATTFEIRNPEDETAATIVADTSELEGMMPQIEEVSQMDPMSQQGGGSGSDGGVVDSILGALGLGDDGDTDQEKVQAAERLVAAYADELQAHLEAEGRWDDVRAAAAENE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVZ9</accession>
    <name>A0A1U7EVZ9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2302A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322869.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVZ9"/>
    <dbReference type="STRING" id="348780.NP_2302A"/>
    <dbReference type="EnsemblBacteria" id="CAI49242">
        <property type="protein sequence ID" value="CAI49242"/>
        <property type="gene ID" value="NP_2302A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701402"/>
    <dbReference type="KEGG" id="nph:NP_2302A"/>
    <dbReference type="HOGENOM" id="CLU_1567200_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="59"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="138"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="170" mass="16132" checksum="7E7D72E3492BF0C3" modified="2017-05-10" version="1">MRPPLARDADSGSDRVRSRSPGVDAGLAAATVAACVLVSLVPALVVADVAGLFGPSVDPLGLTVGVVAAGVVVLPAAFGMGWFVRNAVPAVPGAGILAGGVATAGTYACGLGVCFLGLTAAGLVSGAGLLPAAADSAAVTAAAGGAAVAYTGWLAFPLGCLGGRLYTRTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW63</accession>
    <name>A0A1U7EW63_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC-type transport system ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">abc28a</name>
        <name evidence="6" type="ordered locus">NP_2442A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322938.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW63"/>
    <dbReference type="STRING" id="348780.NP_2442A"/>
    <dbReference type="EnsemblBacteria" id="CAI49312">
        <property type="protein sequence ID" value="CAI49312"/>
        <property type="gene ID" value="NP_2442A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702449"/>
    <dbReference type="KEGG" id="nph:NP_2442A"/>
    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OrthoDB" id="87732at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03230">
        <property type="entry name" value="ABC_DR_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050763">
        <property type="entry name" value="ABC_transporter_ATP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711:SF5">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN NATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="3"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI49312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="306" mass="32358" checksum="2ED9FD837252C54C" modified="2017-05-10" version="1">MVIRASDVRRQYGETVALDGVSLSVDAGEVFALIGPNGAGKTTLIRALTGTTAAEGDISVFGESPESVARHRIGLLPQEFTPAERLTARELIDYYGGLYDETRDTDAILDDVGLTDSAATAYENLSGGQKRRACVGTTLVNDPDLLILDEPTTGIDPRGRRDLWRLIEGLAEGGTTVLLTTHYMAEAEELADRVGMLADGELIALGTPSELVERHGGDSRLVVEADDGTAAKTALRDAGYELLPDERHVAVPAGPREIPSVVEALDDAGADYEGLVWRQPSLDDVYVALTGTDVAAGGEPVTEASP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW82</accession>
    <name>A0A1U7EW82_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Actinobacteria/chloroflexi VLRF1 release factor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2484A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322959.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW82"/>
    <dbReference type="STRING" id="348780.NP_2484A"/>
    <dbReference type="EnsemblBacteria" id="CAI49333">
        <property type="protein sequence ID" value="CAI49333"/>
        <property type="gene ID" value="NP_2484A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701460"/>
    <dbReference type="KEGG" id="nph:NP_2484A"/>
    <dbReference type="eggNOG" id="arCOG01743">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051790_0_0_2"/>
    <dbReference type="OrthoDB" id="124486at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="entry name" value="eRF1 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040783">
        <property type="entry name" value="VLRF1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18859">
        <property type="entry name" value="acVLRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Actinobacteria/chloroflexi VLRF1 release factor" evidence="2">
        <location>
            <begin position="175"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="8"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="292" mass="32660" checksum="436A9B54DE69ECA2" modified="2017-05-10" version="1">MLDDILGRTELKEQIAELEDERDRLAAQLEAESERRADAVSEKQAAQERINRLEDRIADLEGKLQQESESAEIGFRRKETLHGKRRDAVLSRVESFEAKPEGVLTAYVDDDVPDELAEQLGDRTPLVRRAAPCLVCVDDAGVLATALRPPVAPEPFVEWAGRPTVERSWFVPTGRHALAVVRSDIFAVGVYEGDERVDIRTVTSNVKSDHSKGGFSQSRFERIRDEQIQKHIKKASAALDDIDAERRYVVGDRRLIGEFDADATAAVNATGKPKPALEDAVREFWSVRLYGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWA9</accession>
    <name>A0A1U7EWA9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Probable anaerobic dehydrogenase iron-sulfur-binding subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">NP_2584A</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell envelope</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49383.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939543.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWA9"/>
    <dbReference type="STRING" id="348780.NP_2584A"/>
    <dbReference type="EnsemblBacteria" id="CAI49383">
        <property type="protein sequence ID" value="CAI49383"/>
        <property type="gene ID" value="NP_2584A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701497"/>
    <dbReference type="KEGG" id="nph:NP_2584A"/>
    <dbReference type="eggNOG" id="arCOG01501">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043374_5_2_2"/>
    <dbReference type="OrthoDB" id="2837at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009061">
        <property type="term" value="P:anaerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10556">
        <property type="entry name" value="SER_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43518">
        <property type="entry name" value="NITRATE REDUCTASE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43518:SF1">
        <property type="entry name" value="RESPIRATORY NITRATE REDUCTASE 1 BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13247">
        <property type="entry name" value="Fer4_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="12">
        <location>
            <begin position="26"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="12">
        <location>
            <begin position="161"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="12">
        <location>
            <begin position="195"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAI49383.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="401" mass="45439" checksum="A684581474C36FDF" modified="2017-05-10" version="1">MPQVENWQLGREMEYPYEEARPDDQWAGVFDLNKCIECQTCTLACKTTWTHEEGQEHMFWNNVETKPYGSHPVGWDQRLVEELGEMEWDEDGTYEGETIFEAKGAEWIGDGSDIKDDGSAADDMAGFRPSTDDWRYPNLGEDEPAGEEMTPNTHFDESTHPVWFFYLPRICNHCTYAACAGSCPAQAIYKREEDGVVLIDEDNCVAWQQCNEACPYKKAIYNMQEGISQKCVGCYPKTEEGKAPQCFESCIGKIRLHGYINTPDEANVGDPSDERVNPVDFLVHKKEIALPLYPQFGLEPNVYYIPPITAPTDYLTQMFGPGVEDAIEMYQAARDGEEPAVQALLQLMGSTEWSLTGFELDGDEAVGFYEGDEVGRVPITEPTAERDRYDEDEDVYRLDVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWE1</accession>
    <name>A0A1U7EWE1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TRAP-type transport system periplasmic substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tp54a</name>
        <name evidence="1" type="ordered locus">NP_2622A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323027.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWE1"/>
    <dbReference type="STRING" id="348780.NP_2622A"/>
    <dbReference type="EnsemblBacteria" id="CAI49402">
        <property type="protein sequence ID" value="CAI49402"/>
        <property type="gene ID" value="NP_2622A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703342"/>
    <dbReference type="KEGG" id="nph:NP_2622A"/>
    <dbReference type="eggNOG" id="arCOG01802">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_798367_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011852">
        <property type="entry name" value="TRAP_TAXI"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02122">
        <property type="entry name" value="TRAP_TAXI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42941">
        <property type="entry name" value="SLL1037 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42941:SF1">
        <property type="entry name" value="SLL1037 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16868">
        <property type="entry name" value="NMT1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="347" mass="37851" checksum="E62BA8AD4179702A" modified="2017-05-10" version="1">MDPQSSSQSVRMSRRSVLGAVGAGATAALAGCSGGQDGDPLRMRTSTEGTTAYAANQGIAAVINEHTDEIFAEAQTSPGTEANLGALSNEEAEMVYLQNWSAHEIAQGIGQIGDLDFQVAQVFHYYDLPWFFCTATDDIESLSDIESDHSVSPTPEGSGTAPALEHALDIATDGYERQSITYGEQGSAMQEGRLDVGAGTYLNFDIEPGWLQEMMSTVDLTILDVEDGTVDAWEDDDRLLIESFPGSDLEAGEATPAAVPETVYCPTFAYNFVSRADLDYDLVYEFLTVMHEHRMELEEYSAVLASLEDEAFWVENAYDDIPFHPAAADFYEEIGVWSDEFERADES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWF7</accession>
    <name>A0A1U7EWF7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="20">P-type transport ATPase (Probable substrate copper/metal cation)</fullName>
            <ecNumber evidence="20">3.6.3.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">copA</name>
        <name evidence="20" type="synonym">tpa01</name>
        <name evidence="20" type="ordered locus">NP_2668A</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.-" evidence="20"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323050.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWF7"/>
    <dbReference type="STRING" id="348780.NP_2668A"/>
    <dbReference type="EnsemblBacteria" id="CAI49425">
        <property type="protein sequence ID" value="CAI49425"/>
        <property type="gene ID" value="NP_2668A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703348"/>
    <dbReference type="KEGG" id="nph:NP_2668A"/>
    <dbReference type="eggNOG" id="arCOG01576">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02763">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001771_0_3_2"/>
    <dbReference type="OrthoDB" id="8588at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043682">
        <property type="term" value="F:P-type divalent copper transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055070">
        <property type="term" value="P:copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd02094">
        <property type="entry name" value="P-type_ATPase_Cu-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000001">
        <property type="entry name" value="ATPase copper transporting beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000020">
        <property type="entry name" value="Copper-exporting P-type ATPase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000005">
        <property type="entry name" value="Copper-exporting P-type ATPase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017969">
        <property type="entry name" value="Heavy-metal-associated_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006122">
        <property type="entry name" value="HMA_Cu_ion-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00003">
        <property type="entry name" value="copper ion binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520">
        <property type="entry name" value="ATP7, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520:SF8">
        <property type="entry name" value="P-TYPE CU(+) TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00942">
        <property type="entry name" value="CUATPASEI"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01047">
        <property type="entry name" value="HMA_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0186">Copper</keyword>
    <keyword evidence="9" id="KW-0187">Copper transport</keyword>
    <keyword evidence="20" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="162"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="256"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="411"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="460"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="19">
        <location>
            <begin position="3"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="19">
        <location>
            <begin position="71"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="137"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAI49425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="851" mass="88935" checksum="CADFFCA7849E5FD7" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWG4</accession>
    <name>A0A1U7EWG4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">S8 family serine protease</fullName>
            <ecNumber evidence="9">3.4.21.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">NP_2654A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="9"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323043.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWG4"/>
    <dbReference type="STRING" id="348780.NP_2654A"/>
    <dbReference type="EnsemblBacteria" id="CAI49418">
        <property type="protein sequence ID" value="CAI49418"/>
        <property type="gene ID" value="NP_2654A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701513"/>
    <dbReference type="KEGG" id="nph:NP_2654A"/>
    <dbReference type="eggNOG" id="arCOG00702">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_267338_0_0_2"/>
    <dbReference type="OrthoDB" id="103676at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035986">
        <property type="entry name" value="PKD_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF11">
        <property type="entry name" value="CEREVISIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49299">
        <property type="entry name" value="PKD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5 9" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="17"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="8">
        <location>
            <begin position="62"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1143"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI49418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="1193" mass="124620" checksum="836C87FE264090F4" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWM6</accession>
    <name>A0A1U7EWM6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Small CPxCG-related zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2806A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323119.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWM6"/>
    <dbReference type="STRING" id="348780.NP_2806A"/>
    <dbReference type="EnsemblBacteria" id="CAI49494">
        <property type="protein sequence ID" value="CAI49494"/>
        <property type="gene ID" value="NP_2806A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701564"/>
    <dbReference type="KEGG" id="nph:NP_2806A"/>
    <dbReference type="eggNOG" id="arCOG06221">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2629821_0_0_2"/>
    <dbReference type="OrthoDB" id="334705at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="79" mass="9164" checksum="42D30F3745604B9C" modified="2017-05-10" version="1">MTYTCRNCKRTFTNELQYELHRDTCSSEALVCECCGEQFSERRATRDGWHYECPNDDCDGTGIDDDLHSVRDFSVTTPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWQ5</accession>
    <name>A0A1U7EWQ5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Probable GTP 3',8-cyclase</fullName>
            <ecNumber evidence="11">4.1.99.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Molybdenum cofactor biosynthesis protein A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 13" type="primary">moaA</name>
        <name evidence="13" type="ordered locus">NP_2822A</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>GTP + AH2 + S-adenosyl-L-methionine = (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + A + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49576"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.1.99.22"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="11">Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 [4Fe-4S] cluster coordinated with 3 cysteines and the GTP-derived substrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the radical SAM superfamily. MoaA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.22" evidence="11"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323127.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWQ5"/>
    <dbReference type="STRING" id="348780.NP_2822A"/>
    <dbReference type="EnsemblBacteria" id="CAI49502">
        <property type="protein sequence ID" value="CAI49502"/>
        <property type="gene ID" value="NP_2822A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702922"/>
    <dbReference type="KEGG" id="nph:NP_2822A"/>
    <dbReference type="eggNOG" id="arCOG00930">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009273_0_1_2"/>
    <dbReference type="OrthoDB" id="6925at2157"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061799">
        <property type="term" value="F:cyclic pyranopterin monophosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061798">
        <property type="term" value="F:GTP 3',8'-cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01225_A">
        <property type="entry name" value="MoaA_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040064">
        <property type="entry name" value="MoaA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013485">
        <property type="entry name" value="MoaA_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000385">
        <property type="entry name" value="MoaA_NifB_PqqE_Fe-S-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010505">
        <property type="entry name" value="MoaA_twitch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050105">
        <property type="entry name" value="MoCo_biosynth_MoaA/MoaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02668">
        <property type="entry name" value="moaA_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001199">
        <property type="entry name" value="PRK00164.2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960:SF0">
        <property type="entry name" value="MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960">
        <property type="entry name" value="MOLYBDOPTERIN COFACTOR SYNTHESIS PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06463">
        <property type="entry name" value="Mob_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01383">
        <property type="entry name" value="cyclic_pyranopterin_phosphate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01386">
        <property type="entry name" value="main_SPASM_domain-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01305">
        <property type="entry name" value="MOAA_NIFB_PQQE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 11" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 11" id="KW-0408">Iron</keyword>
    <keyword evidence="6 11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 11 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="12">
        <location>
            <begin position="5"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="260"/>
            <end position="262"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAI49502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="326" mass="36683" checksum="8CC7151C45689923" modified="2017-05-10" version="1">MLEDGFGRETTGVRISLTDRCNFDCVYCHNEGLGDTRGPMEPAEDEMSTDDVIRFLEVAAEFDIQSAKFTGGEPMLREDLETIVRRTPDSIETSLTTNGTFLPDRATALVDAGLERVNVSQDALSPKAFQTVTKSSAYDDVMAGIDAALDAGLAPVKLNMVVFEQTAGYVPEMVDHVADNEGLQLQLIEYMPELAGRPEWAVDIERVHEWLADQADRVDRREMHGRNRYFVGEDDPDRPGGMVEIVDPVGNADFCANCHRVRVTHEGYLKGCLNRNDDLKSMGEMTKPEIREAFRETVDERVPYYGEYMVQDENGEWRFNEEYISA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWS4</accession>
    <name>A0A1U7EWS4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">MTH865 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2882A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323157.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWS4"/>
    <dbReference type="STRING" id="348780.NP_2882A"/>
    <dbReference type="EnsemblBacteria" id="CAI49532">
        <property type="protein sequence ID" value="CAI49532"/>
        <property type="gene ID" value="NP_2882A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701582"/>
    <dbReference type="KEGG" id="nph:NP_2882A"/>
    <dbReference type="eggNOG" id="arCOG02797">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_193458_0_0_2"/>
    <dbReference type="OrthoDB" id="335595at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.80">
        <property type="entry name" value="MTH865-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036825">
        <property type="entry name" value="MTH865-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024093">
        <property type="entry name" value="Uncharacterised_MTH865"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07747">
        <property type="entry name" value="MTH865"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69025">
        <property type="entry name" value="Hypothetical protein MTH865"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="84" mass="9287" checksum="C7510500DCA2E34D" modified="2017-05-10" version="1">MVDKDDLREQFEDAFEGADYPVNSPMDLVPALPQGPGTKFESGDFSMTAMELNTKAGGNQDFPYESSDELVEDIMNGLEEEGYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWT9</accession>
    <name>A0A1U7EWT9_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">dna2</name>
        <name evidence="23" type="ordered locus">NP_2944A</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323187.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWT9"/>
    <dbReference type="STRING" id="348780.NP_2944A"/>
    <dbReference type="DNASU" id="3701606"/>
    <dbReference type="EnsemblBacteria" id="CAI49563">
        <property type="protein sequence ID" value="CAI49563"/>
        <property type="gene ID" value="NP_2944A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701606"/>
    <dbReference type="KEGG" id="nph:NP_2944A"/>
    <dbReference type="eggNOG" id="arCOG00795">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005032_0_0_2"/>
    <dbReference type="OrthoDB" id="45637at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050534">
        <property type="entry name" value="Coronavir_polyprotein_1ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014808">
        <property type="entry name" value="DNA_replication_fac_Dna2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788:SF8">
        <property type="entry name" value="DNA-BINDING PROTEIN SMUBP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08696">
        <property type="entry name" value="Dna2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="16" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11 23" id="KW-0347">Helicase</keyword>
    <keyword evidence="10 23" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0408">Iron</keyword>
    <keyword evidence="14" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA replication factor Dna2 N-terminal" evidence="20">
        <location>
            <begin position="40"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="21">
        <location>
            <begin position="512"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="21">
        <location>
            <begin position="610"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="22">
        <location>
            <begin position="681"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF08696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF13086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="CAI49563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="883" mass="97302" checksum="AB08C30C7F7515BD" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWU1</accession>
    <name>A0A1U7EWU1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">N-methylhydantoinase (ATP-hydrolyzing) A</fullName>
            <ecNumber evidence="4">3.5.2.14</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hyuA1</name>
        <name evidence="4" type="ordered locus">NP_2954A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.2.14" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323192.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWU1"/>
    <dbReference type="STRING" id="348780.NP_2954A"/>
    <dbReference type="EnsemblBacteria" id="CAI49568">
        <property type="protein sequence ID" value="CAI49568"/>
        <property type="gene ID" value="NP_2954A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702854"/>
    <dbReference type="KEGG" id="nph:NP_2954A"/>
    <dbReference type="eggNOG" id="arCOG01511">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002157_1_2_2"/>
    <dbReference type="OrthoDB" id="8261at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017168">
        <property type="term" value="F:5-oxoprolinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047423">
        <property type="term" value="F:N-methylhydantoinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049517">
        <property type="entry name" value="ACX-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008040">
        <property type="entry name" value="Hydant_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002821">
        <property type="entry name" value="Hydantoinase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="5-OXOPROLINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365:SF23">
        <property type="entry name" value="HYPOTHETICAL 5-OXOPROLINASE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19278">
        <property type="entry name" value="Hydant_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05378">
        <property type="entry name" value="Hydant_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01968">
        <property type="entry name" value="Hydantoinase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydantoinase/oxoprolinase N-terminal" evidence="2">
        <location>
            <begin position="17"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Hydantoinase A/oxoprolinase" evidence="1">
        <location>
            <begin position="211"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="Acetophenone carboxylase-like C-terminal" evidence="3">
        <location>
            <begin position="521"/>
            <end position="661"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="680" mass="71519" checksum="0EC891A90B174F40" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWY5</accession>
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        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">NP_3066A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323246.1">
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWY5"/>
    <dbReference type="STRING" id="348780.NP_3066A"/>
    <dbReference type="EnsemblBacteria" id="CAI49624">
        <property type="protein sequence ID" value="CAI49624"/>
        <property type="gene ID" value="NP_3066A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701643"/>
    <dbReference type="KEGG" id="nph:NP_3066A"/>
    <dbReference type="eggNOG" id="arCOG07542">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_193443_0_0_2"/>
    <dbReference type="OrthoDB" id="304630at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058309">
        <property type="entry name" value="DUF7996"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25959">
        <property type="entry name" value="DUF7996"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="74" mass="7974" checksum="2A59700843940C9A" modified="2017-05-10" version="1">MSTPALGERVPDWALGSLLLAGTISIAGITDYFLTRAGYEMVGTVIWTLAYGAAFAVVWFVWLRHIDLTGPESG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX03</accession>
    <name>A0A1U7EX03_NATPD</name>
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        <recommendedName>
            <fullName evidence="2">GMP reductase</fullName>
            <ecNumber evidence="1">1.7.1.7</ecNumber>
        </recommendedName>
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    <gene>
        <name evidence="6" type="primary">guaB2</name>
        <name evidence="6" type="ordered locus">NP_3080A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.7.1.7" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX03"/>
    <dbReference type="STRING" id="348780.NP_3080A"/>
    <dbReference type="EnsemblBacteria" id="CAI49631">
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        <property type="gene ID" value="NP_3080A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702785"/>
    <dbReference type="KEGG" id="nph:NP_3080A"/>
    <dbReference type="eggNOG" id="arCOG00612">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022552_5_0_2"/>
    <dbReference type="OrthoDB" id="324877at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003920">
        <property type="term" value="F:GMP reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Inosine-5'-monophosphate dehydrogenase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001295">
        <property type="entry name" value="Dihydroorotate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050139">
        <property type="entry name" value="GMP_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001093">
        <property type="entry name" value="IMP_DH_GMPRt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43170">
        <property type="entry name" value="GMP REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43170:SF5">
        <property type="entry name" value="GMP REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="DHODEHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IMP dehydrogenase/GMP reductase" evidence="5">
        <location>
            <begin position="6"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI49631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="345" mass="35346" checksum="0266379E0946D8C3" modified="2017-05-10" version="1">MPERTGLSYDDVLLVPQRSPVDSRDNVELTTTLADGLTLSLPVTTAAMDTVTEAEMARAVGEAGGLGVLHRFLPAEEQAAMVASVADDGVPVAAAVGIAEPHTERAAALVEAGVDMLVVDVAHGHMERTLDVTAELASAFPETALCAGNVATPDGVADLAEAGADCVKVGVGPGSHCTTREVTGFGVPQFTAVDRCADAASAADVTVIADGGIQSSGDAVKSLLAGADAVMMGGYFAGCDESPGETVEIDGQTYKRSRGMSTAAAAEDREDKDVDVVADEGVEAVTEYVGPVNERLDEFAAGIRSGLSYAGAHDLETARENAAFMEVTPTTDARNAAHGVARKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX34</accession>
    <name>A0A1U7EX34_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3128A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
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    <dbReference type="STRING" id="348780.NP_3128A"/>
    <dbReference type="EnsemblBacteria" id="CAI49655">
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        <property type="gene ID" value="NP_3128A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701661"/>
    <dbReference type="KEGG" id="nph:NP_3128A"/>
    <dbReference type="eggNOG" id="arCOG02079">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028008_1_0_2"/>
    <dbReference type="OrthoDB" id="56770at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35902:SF3">
        <property type="entry name" value="NPCBM-ASSOCIATED, NEW3 DOMAIN OF ALPHA-GALACTOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35902">
        <property type="entry name" value="S-LAYER DOMAIN-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="507"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="529" mass="56041" checksum="968344A1F742741F" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX73</accession>
    <name>A0A1U7EX73_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable glycosyltransferase, type 1</fullName>
            <ecNumber evidence="2">2.4.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">gth1</name>
        <name evidence="2" type="ordered locus">NP_3218A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.4.-.-" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323322.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX73"/>
    <dbReference type="STRING" id="348780.NP_3218A"/>
    <dbReference type="EnsemblBacteria" id="CAI49700">
        <property type="protein sequence ID" value="CAI49700"/>
        <property type="gene ID" value="NP_3218A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701687"/>
    <dbReference type="KEGG" id="nph:NP_3218A"/>
    <dbReference type="eggNOG" id="arCOG01403">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_2_0_2"/>
    <dbReference type="OrthoDB" id="238665at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03817">
        <property type="entry name" value="GT4_UGDG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050194">
        <property type="entry name" value="Glycosyltransferase_grp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45947">
        <property type="entry name" value="SULFOQUINOVOSYL TRANSFERASE SQD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45947:SF3">
        <property type="entry name" value="SULFOQUINOVOSYL TRANSFERASE SQD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13692">
        <property type="entry name" value="Glyco_trans_1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase subfamily 4-like N-terminal" evidence="1">
        <location>
            <begin position="15"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="367" mass="39553" checksum="36F9FE497A4D2BDA" modified="2017-05-10" version="1">MRTVAAFTDTYLPTVNGVSYTASTWRDRWCRRGGRMPLVYPAADGYQPEDGEHPVGSVRFPWYEGFRLGRPAVPDAVERAAPDVVHAHTPFALGLAAARLAARSDAPLIVSYHTPVSEYAGYVSDAFADAVGVAADRYERWFLNRADAVVAPSQTAAERIDAEETPVYVVSNGVDTDRFRPVGEHAVTAFRDRYGLPDGPLVGYTGRHGREKRLGDVLAATAGLDAGVVIAGAGPATPELRRRTAAREDVRLLGFLERNELPAFYTALDAFAFPSPVETQGLVALEAIACGTPVVAADGGALPETVDSGETGCHFPVGDVRAFRTALRQTLARTDVLSDRCVARREQLAVERAIDRLGDVYETVTHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXD3</accession>
    <name>A0A1U7EXD3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Metal-dependent hydrolase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3476A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323449.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXD3"/>
    <dbReference type="STRING" id="348780.NP_3476A"/>
    <dbReference type="EnsemblBacteria" id="CAI49829">
        <property type="protein sequence ID" value="CAI49829"/>
        <property type="gene ID" value="NP_3476A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701783"/>
    <dbReference type="KEGG" id="nph:NP_3476A"/>
    <dbReference type="eggNOG" id="arCOG00497">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070010_0_1_2"/>
    <dbReference type="OrthoDB" id="337606at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050114">
        <property type="entry name" value="UPF0173_UPF0282_UlaG_hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43546:SF8">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43546">
        <property type="entry name" value="UPF0173 METAL-DEPENDENT HYDROLASE MJ1163-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13483">
        <property type="entry name" value="Lactamase_B_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="233" mass="25004" checksum="2A0E6CD8FD53C9A7" modified="2017-05-10" version="1">MVEHEGVTVSWLGYATLRIDDGETVVYVDPGRYGVLDGYDGGDGDVVCVSHGHHYDSAAIEQVAGDGATLVYFEGINTHHIDRDVARPASLSLSTRTVDAEADIAVGDAIIRTTAAYNDPDGPHVRPAGEPYHPKGRGCGFHITLGGVSVYWPGDTDVLEGHEHLDVDVFCPPIGGRYTMDRHEAADLAVALAPEIVLPVHYDTFDEIEADAAAFADELESRGVDVVIEPPTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXD9</accession>
    <name>A0A1U7EXD9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UPF0047 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3454A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0047 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323438.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXD9"/>
    <dbReference type="STRING" id="348780.NP_3454A"/>
    <dbReference type="EnsemblBacteria" id="CAI49818">
        <property type="protein sequence ID" value="CAI49818"/>
        <property type="gene ID" value="NP_3454A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701778"/>
    <dbReference type="KEGG" id="nph:NP_3454A"/>
    <dbReference type="eggNOG" id="arCOG04214">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096980_1_1_2"/>
    <dbReference type="OrthoDB" id="6663at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.460">
        <property type="entry name" value="YjbQ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001602">
        <property type="entry name" value="UPF0047_YjbQ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035917">
        <property type="entry name" value="YjbQ-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00149">
        <property type="entry name" value="TIGR00149_YjbQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30615">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YJBQ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30615:SF8">
        <property type="entry name" value="UPF0047 PROTEIN C4A8.02C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01894">
        <property type="entry name" value="YjbQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004681">
        <property type="entry name" value="UCP004681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111038">
        <property type="entry name" value="YjbQ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01314">
        <property type="entry name" value="UPF0047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="126" mass="13673" checksum="9ADE201DED6EE9F1" modified="2017-05-10" version="1">MPSFTVETDREIQCIDITDRVTEALPDDHDGVTTVASRHTTAAVAINEAEPRLLGDVEAFLRENVADEGWDHDALDGNADGHLRALLVGPSEQVPVADGSPDLGTWQSVLLVECDGPRSRTVTVRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXE3</accession>
    <name>A0A1U7EXE3_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA gyrase subunit A</fullName>
            <ecNumber evidence="9">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 12" type="primary">gyrA</name>
        <name evidence="12" type="ordered locus">NP_3500A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterotetramer composed of ParC and ParE.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="9">Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the type II topoisomerase GyrA/ParC subunit family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="9"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323461.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXE3"/>
    <dbReference type="STRING" id="348780.NP_3500A"/>
    <dbReference type="EnsemblBacteria" id="CAI49841">
        <property type="protein sequence ID" value="CAI49841"/>
        <property type="gene ID" value="NP_3500A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702789"/>
    <dbReference type="KEGG" id="nph:NP_3500A"/>
    <dbReference type="eggNOG" id="arCOG04367">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002977_6_1_2"/>
    <dbReference type="OrthoDB" id="371943at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009330">
        <property type="term" value="C:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00187">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.268.10:FF:000001">
        <property type="entry name" value="DNA gyrase subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.40:FF:000002">
        <property type="entry name" value="DNA gyrase subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.199.10:FF:000001">
        <property type="entry name" value="DNA gyrase subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.90:FF:000005">
        <property type="entry name" value="DNA topoisomerase 4 subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.90">
        <property type="entry name" value="DNA gyrase/topoisomerase IV, subunit A, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.199.10">
        <property type="entry name" value="Topoisomerase II, domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.268.10">
        <property type="entry name" value="Topoisomerase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01897">
        <property type="entry name" value="GyrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005743">
        <property type="entry name" value="GyrA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006691">
        <property type="entry name" value="GyrA/parC_rep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035516">
        <property type="entry name" value="Gyrase/topoIV_suA_C"/>
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    </dbReference>
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        <property type="match status" value="6"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Type II DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        </location>
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            <position position="130"/>
        </location>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00008263"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00063644"/>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS52040"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI49841.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="821" mass="90642" checksum="C6DD7E9DFBD1C3A0" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXI2</accession>
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        <submittedName>
            <fullName evidence="2">Major facilitator superfamily transport protein</fullName>
        </submittedName>
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        <name evidence="2" type="primary">tp59</name>
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        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <lineage>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
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        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
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        <location>
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            <end position="127"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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        </location>
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        </location>
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        <location>
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        </location>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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            <end position="393"/>
        </location>
    </feature>
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        <location>
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            <end position="424"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI49868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="430" mass="45408" checksum="E903A9222A898179" modified="2017-05-10" version="1">MPLGSRTLFRVTTETRHMDSTSPAGRTPTKAAVAFAVYVSVLTAAYYYNLTFIQLGLTDLGLERLGLSPERVAVGMGVLALVTLCTTLVSGYLMDRLGVGANPLSKFRVLFGIVVLQVATTYAVTAVTSFEQFLTWIALCSLLLGTAIPFAFSLLLDLVRPGIRGYAAGAVAGCAFFLAALFPFEWTIESFVPSAVAVLAPVALLLGVLSVPSIGDWVSGSRTDDSPGRQPVGVTQSGMLTAAFVGGVVLLFGAFFVDSLGFVRIIESEYVEASWQSSELGTRLLIAMTHVIGGLAAGVAYAKLRYHWLFVATFVLFAAAQFLYVYDAAVGGPAALAAVRPLLYVLAVSCYTTVAFALWPDLATPDRVGTYTAVGIGIGGWLATFTSTAAALVSERAEIALEVHLFIVGSVSVAFLLITGVLMYRAVQTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXL1</accession>
    <name>A0A1U7EXL1_NATPD</name>
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        <submittedName>
            <fullName evidence="3">Acetyl-CoA C-acetyltransferase small subunit</fullName>
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        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3652A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.9" evidence="3"/>
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        <property type="protein sequence ID" value="CAI49917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXL1"/>
    <dbReference type="STRING" id="348780.NP_3652A"/>
    <dbReference type="EnsemblBacteria" id="CAI49917">
        <property type="protein sequence ID" value="CAI49917"/>
        <property type="gene ID" value="NP_3652A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701834"/>
    <dbReference type="KEGG" id="nph:NP_3652A"/>
    <dbReference type="eggNOG" id="arCOG04614">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155008_0_0_2"/>
    <dbReference type="OrthoDB" id="213852at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003985">
        <property type="term" value="F:acetyl-CoA C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="entry name" value="ChsH2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01796">
        <property type="entry name" value="OB_ChsH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ChsH2 C-terminal OB-fold" evidence="2">
        <location>
            <begin position="31"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="120" mass="13199" checksum="2775B3DBDA9F6D35" modified="2017-05-10" version="1">MTMEAYRYPDGSITYPGHPVGPNGDEPVGTVDLSDHTAEVLTWTTSHATPVGVREPNHLAIVEFRVDGQAVRALGQLTTGDVAIGDEVRPVYHEELRDPEAGIRDPESQSWDGYRFEPVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXL6</accession>
    <name>A0A1U7EXL6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cold shock protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="5" type="primary">cspA2</name>
        <name evidence="5" type="ordered locus">NP_3636A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
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            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXL6"/>
    <dbReference type="STRING" id="348780.NP_3636A"/>
    <dbReference type="EnsemblBacteria" id="CAI49909">
        <property type="protein sequence ID" value="CAI49909"/>
        <property type="gene ID" value="NP_3636A"/>
    </dbReference>
    <dbReference type="GeneID" id="82180847"/>
    <dbReference type="KEGG" id="nph:NP_3636A"/>
    <dbReference type="eggNOG" id="arCOG02984">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117621_7_2_2"/>
    <dbReference type="OrthoDB" id="202422at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012156">
        <property type="entry name" value="Cold_shock_CspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050181">
        <property type="entry name" value="Cold_shock_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11544">
        <property type="entry name" value="COLD SHOCK DOMAIN CONTAINING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002599">
        <property type="entry name" value="Cold_shock_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00050">
        <property type="entry name" value="COLDSHOCK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CSD" evidence="4">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="68" mass="7407" checksum="D704C0FCFB07EB3C" modified="2017-05-10" version="1">MANGKVDFFNDTGGYGFISTDDGDLDDDEDVFFHMEDVGGEDLTEGTEVEFDIESSPKGPRAANVVRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXM0</accession>
    <name>A0A1U7EXM0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transducer protein Htr8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">htr8</name>
        <name evidence="8" type="ordered locus">NP_3638A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323529.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXM0"/>
    <dbReference type="STRING" id="348780.NP_3638A"/>
    <dbReference type="EnsemblBacteria" id="CAI49910">
        <property type="protein sequence ID" value="CAI49910"/>
        <property type="gene ID" value="NP_3638A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702850"/>
    <dbReference type="KEGG" id="nph:NP_3638A"/>
    <dbReference type="eggNOG" id="arCOG02320">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_18_2"/>
    <dbReference type="OrthoDB" id="8523at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.950">
        <property type="entry name" value="Methyl-accepting chemotaxis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004090">
        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32089:SF112">
        <property type="entry name" value="LYSOZYME-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32089">
        <property type="entry name" value="METHYL-ACCEPTING CHEMOTAXIS PROTEIN MCPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00260">
        <property type="entry name" value="CHEMTRNSDUCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58104">
        <property type="entry name" value="Methyl-accepting chemotaxis protein (MCP) signaling domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0807">Transducer</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="7">
        <location>
            <begin position="310"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="595"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="202"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="311"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="618" mass="65236" checksum="0C44572E98522123" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXP4</accession>
    <name>A0A1U7EXP4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3786A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323601.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXP4"/>
    <dbReference type="EnsemblBacteria" id="CAI49984">
        <property type="protein sequence ID" value="CAI49984"/>
        <property type="gene ID" value="NP_3786A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701863"/>
    <dbReference type="KEGG" id="nph:NP_3786A"/>
    <dbReference type="HOGENOM" id="CLU_2505050_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="85" mass="9156" checksum="DDB5C97BBD5E7C02" modified="2017-05-10" version="1">MSRRQTVDRAAVAVFAAFSIAGLFVFRAAMGWSVTDPYVPAGVHEALPATAWISVVPLFVLVGLLAAIFVYRLSGVPMPGSRYRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXS3</accession>
    <name>A0A1U7EXS3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">SWIM zinc finger domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_3842A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323629.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXS3"/>
    <dbReference type="STRING" id="348780.NP_3842A"/>
    <dbReference type="EnsemblBacteria" id="CAI50012">
        <property type="protein sequence ID" value="CAI50012"/>
        <property type="gene ID" value="NP_3842A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701882"/>
    <dbReference type="KEGG" id="nph:NP_3842A"/>
    <dbReference type="eggNOG" id="arCOG03429">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017027_0_0_2"/>
    <dbReference type="OrthoDB" id="41163at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04434">
        <property type="entry name" value="SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="3">
        <location>
            <begin position="50"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="307"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="324"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="670" mass="76881" checksum="30436DB890789F43" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXV3</accession>
    <name>A0A1U7EXV3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ATP-dependent helicase</fullName>
            <ecNumber evidence="8">3.6.4.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">NP_3872A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
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            <dbReference type="PubMed" id="16169924"/>
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</entry>
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        </source>
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        <source>
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    </evidence>
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<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY11</accession>
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            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
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            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
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        <text evidence="1">Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.</text>
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    <dbReference type="Proteomes" id="UP000002698">
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:heme binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
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    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="RHOD"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="2"/>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="16"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="8">
        <location>
            <begin position="157"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI50093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="795" mass="87119" checksum="31FE6023D879132A" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY24</accession>
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        </submittedName>
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    <organism evidence="1 2">
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            <taxon>Methanobacteriati</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
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            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
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        <property type="gene ID" value="NP_4016A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701941"/>
    <dbReference type="KEGG" id="nph:NP_4016A"/>
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    <dbReference type="HOGENOM" id="CLU_110996_0_0_2"/>
    <dbReference type="OrthoDB" id="324613at2157"/>
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    </dbReference>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="191" mass="20621" checksum="C7F144602FBC119E" modified="2017-05-10" version="1">MRGQAHTVEAFVAAVLIVGGLVFATQATAVTPLSASTSNQHIENQQEATVEGLLATAEREGMLQEAVLFWDPDEEEFQGVPDQGTYAAGPPNDFGEALNKTLLERQIAFNIDVHYAEPDGGVGTESMVRMGEPSDNAVAATRALGVYDSSELTSDGYEGEQLDGTEFYADDIDDNSELYTVIEVEVVVWRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY62</accession>
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        <submittedName>
            <fullName evidence="2">BsuPI domain protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">NP_4114A</name>
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    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <lineage>
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            <taxon>Methanobacteriati</taxon>
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            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="CAI50148"/>
        <property type="gene ID" value="NP_4114A"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2360">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="IPI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12690">
        <property type="entry name" value="BsuPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Intracellular proteinase inhibitor BsuPI" evidence="1">
        <location>
            <begin position="10"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="108" mass="11762" checksum="55F6C58E7EC80D39" modified="2017-05-10" version="1">MLDTALEVTVGDGVSFRFTVTNAGDTPVELTFRDACRADIEVREADTVVWRYSDGRAFAQATSTADLQPGETATFERSWPSPAPGDYTAEATLRVLEGDIRARTPFSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY71</accession>
    <name>A0A1U7EY71_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC-type transport system periplasmic substrate-binding protein (Probable substrate branched-chain amino acids)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">livJ1</name>
        <name evidence="3" type="synonym">abc05s</name>
        <name evidence="3" type="ordered locus">NP_4140A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323777.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY71"/>
    <dbReference type="STRING" id="348780.NP_4140A"/>
    <dbReference type="EnsemblBacteria" id="CAI50161">
        <property type="protein sequence ID" value="CAI50161"/>
        <property type="gene ID" value="NP_4140A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702381"/>
    <dbReference type="KEGG" id="nph:NP_4140A"/>
    <dbReference type="eggNOG" id="arCOG01021">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053087_0_0_2"/>
    <dbReference type="OrthoDB" id="21336at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051010">
        <property type="entry name" value="BCAA_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028081">
        <property type="entry name" value="Leu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30483">
        <property type="entry name" value="LEUCINE-SPECIFIC-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30483:SF6">
        <property type="entry name" value="PERIPLASMIC BINDING PROTEIN OF ABC TRANSPORTER FOR NATURAL AMINO ACIDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13458">
        <property type="entry name" value="Peripla_BP_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Leucine-binding protein" evidence="2">
        <location>
            <begin position="39"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-binding protein" evidence="2">
        <location>
            <begin position="247"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="434" mass="45077" checksum="14310CDDACA45A88" modified="2017-05-10" version="1">MTRHINRRNVLKAAGAASMVALAGCNGNGNGNGNGDGVAQTPDSVMVVGFPQSGIQLFRDYYGEFADDYDVDIIVPDGLIDSDLPGDVDNDMDNVIGTEPAADGPGADFFADSYEDEYGEAPSVFTSHAYDAMAVSVLAAVAAGENDGEAIRDRMATVANPNGEEFGPAELPEAVETVAAGDSINYQGASSSVDFDENGDIVSAAYDIVDFQGGQLEVVDSLELEAGEIGDVTAEDPVGVDEFEAMVGVLMPETGDLGPLGGPIRDGALLAATQINDADLNVSVDTSVEDTQTDPTAAISSAESLVNAGYPAVVGPAGSDQNFPVSTEVYVPNSVVGISPASTAPGVTELDDDGYIFRTAPSDALQGPVMAEVVMDRLGHDNTATLYLNDEYGQALEASYVNGFEDLGGEVFQQVSFEPEQPSYSSQWAEVLDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYA5</accession>
    <name>A0A1U7EYA5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Putative DNA 3'-5' helicase Rad25</fullName>
            <ecNumber evidence="9">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">rad25c</name>
        <name evidence="9" type="synonym">rad25</name>
        <name evidence="12" type="synonym">rhl</name>
        <name evidence="12" type="ordered locus">NP_4200A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 14">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the helicase family. RAD25/XPB subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="9"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50191.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323807.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYA5"/>
    <dbReference type="STRING" id="348780.NP_4200A"/>
    <dbReference type="EnsemblBacteria" id="CAI50191">
        <property type="protein sequence ID" value="CAI50191"/>
        <property type="gene ID" value="NP_4200A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703103"/>
    <dbReference type="KEGG" id="nph:NP_4200A"/>
    <dbReference type="eggNOG" id="arCOG00874">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008213_4_0_2"/>
    <dbReference type="OrthoDB" id="8379at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01489">
        <property type="entry name" value="Helicase_Rad25_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050615">
        <property type="entry name" value="ATP-dep_DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032438">
        <property type="entry name" value="ERCC3_RAD25_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030882">
        <property type="entry name" value="Helicase_Rad25_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11274:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION AND DNA REPAIR FACTOR IIH HELICASE SUBUNIT XPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11274">
        <property type="entry name" value="RAD25/XP-B DNA REPAIR HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16203">
        <property type="entry name" value="ERCC3_RAD25_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00851">
        <property type="entry name" value="XRODRMPGMNTB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 9 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="269"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="470"/>
            <end position="619"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI50191.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="624" mass="68867" checksum="3BEB66AFA800F514" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYE9</accession>
    <name>A0A1U7EYE9_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidoglycan-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4336A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323875.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYE9"/>
    <dbReference type="STRING" id="348780.NP_4336A"/>
    <dbReference type="EnsemblBacteria" id="CAI50259">
        <property type="protein sequence ID" value="CAI50259"/>
        <property type="gene ID" value="NP_4336A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702034"/>
    <dbReference type="KEGG" id="nph:NP_4336A"/>
    <dbReference type="eggNOG" id="arCOG06240">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132453_1_0_2"/>
    <dbReference type="OrthoDB" id="280951at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043860">
        <property type="entry name" value="DUF5822"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19139">
        <property type="entry name" value="DUF5822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="92" mass="9846" checksum="4566CC023D33EF85" modified="2017-05-10" version="1">MPTRVETTDPDGVDYGWVMQTTFVLTIIVGAPVVAALSLLVELPTWGQRANFAIRVGAVVWLCLGIGVFFYARRDAGAGQADSDGESGDETR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYJ4</accession>
    <name>A0A1U7EYJ4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4438A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323925.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYJ4"/>
    <dbReference type="STRING" id="348780.NP_4438A"/>
    <dbReference type="EnsemblBacteria" id="CAI50310">
        <property type="protein sequence ID" value="CAI50310"/>
        <property type="gene ID" value="NP_4438A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702063"/>
    <dbReference type="KEGG" id="nph:NP_4438A"/>
    <dbReference type="eggNOG" id="arCOG04645">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1639912_0_0_2"/>
    <dbReference type="OrthoDB" id="213717at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055945">
        <property type="entry name" value="DUF7523"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24367">
        <property type="entry name" value="DUF7523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="160" mass="16309" checksum="943AEB84290F2FD7" modified="2017-05-10" version="1">MTVAADTRAAVRERPFLETALRAGVVNYTAAARVLDVGDTEAVAAALRRYAEAADEYSPPKTDASVSMRSGIGRSDDNSNGLFSVGDTCFAADGGDLTAIVAEGDVEPGVLEPVLGRLRTADIGVEAAAAGDGYLAVVVGRRDGADAVRAVEGSLGQATH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYK1</accession>
    <name>A0A1U7EYK1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cysteine--tRNA ligase</fullName>
            <ecNumber evidence="12">6.1.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Cysteinyl-tRNA synthetase</fullName>
            <shortName evidence="12">CysRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12 15" type="primary">cysS</name>
        <name evidence="15" type="ordered locus">NP_4440A</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>tRNA(Cys) + L-cysteine + ATP = L-cysteinyl-tRNA(Cys) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:17773"/>
            <dbReference type="Rhea" id="RHEA-COMP:9661"/>
            <dbReference type="Rhea" id="RHEA-COMP:9679"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78517"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.16" evidence="12"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323926.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYK1"/>
    <dbReference type="SMR" id="A0A1U7EYK1"/>
    <dbReference type="STRING" id="348780.NP_4440A"/>
    <dbReference type="EnsemblBacteria" id="CAI50311">
        <property type="protein sequence ID" value="CAI50311"/>
        <property type="gene ID" value="NP_4440A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702655"/>
    <dbReference type="KEGG" id="nph:NP_4440A"/>
    <dbReference type="eggNOG" id="arCOG00486">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013528_0_2_2"/>
    <dbReference type="OrthoDB" id="9445at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004817">
        <property type="term" value="F:cysteine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006423">
        <property type="term" value="P:cysteinyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00672">
        <property type="entry name" value="CysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1910">
        <property type="entry name" value="Cysteine-tRNA ligase, C-terminal anti-codon recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00041">
        <property type="entry name" value="Cys_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015803">
        <property type="entry name" value="Cys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015273">
        <property type="entry name" value="Cys-tRNA-synt_Ia_DALR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024909">
        <property type="entry name" value="Cys-tRNA/MSH_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032678">
        <property type="entry name" value="tRNA-synt_1_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00435">
        <property type="entry name" value="cysS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890:SF3">
        <property type="entry name" value="CYSTEINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890">
        <property type="entry name" value="CYSTEINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09190">
        <property type="entry name" value="DALR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01406">
        <property type="entry name" value="tRNA-synt_1e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00983">
        <property type="entry name" value="TRNASYNTHCYS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00840">
        <property type="entry name" value="DALR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 12 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Cysteinyl-tRNA synthetase class Ia DALR" evidence="14">
        <location>
            <begin position="379"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="445"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="12">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="12">
        <location>
            <begin position="292"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAI50311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="497" mass="55384" checksum="78E38F2665D84994" modified="2017-05-10" version="1">MTLRVSNTLSGEREEFEPADPDNVLLYYCGLTVSDYAHLGHARTWVHVDVIHRWLEALGYDVRHVENITDVNEKIVARVGEPADGDTFGDDEGDVATHFIDQLLSDMRSLNLKRAEVYPRVSEHVPEIIDLIETLVERGYAYESNGSVYFDVTEFEEYGKLSNQRLEAVEAQGPETEQAEKRHPADFALWKAGGVSESALEEHRDADCEYATESPAGQTWASPWGEGRPGWHIECSAMSMTHLDDSIDIHMGGRDLVFPHHENEIAQSEAATGEQFARYWLHADLFQMDDEKMSSSLGNFIPVSEAVARFGVNPLRMFFLSASYNSTQTYSEAAIEEALERWDRLAAAYERAAAAADSPAAGTKVDSPLGEAVEAAEAAFTEAMNDDFNTREALSALFDLASAINSHLDDRDRYDYRALRRGIEAFERLGGDVLGFDFDGSADGEAALAGELVELLLEVREAEREAENYERADKIRDDIEALGVEIQDTDDGAEYRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYK8</accession>
    <name>A0A1U7EYK8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Malate synthase</fullName>
            <ecNumber evidence="6">2.3.3.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">aceB</name>
        <name evidence="6" type="ordered locus">NP_4430A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="2.3.3.9" evidence="6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323921.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYK8"/>
    <dbReference type="STRING" id="348780.NP_4430A"/>
    <dbReference type="EnsemblBacteria" id="CAI50306">
        <property type="protein sequence ID" value="CAI50306"/>
        <property type="gene ID" value="NP_4430A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702061"/>
    <dbReference type="KEGG" id="nph:NP_4430A"/>
    <dbReference type="eggNOG" id="arCOG00760">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_629476_0_0_2"/>
    <dbReference type="OrthoDB" id="191256at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004474">
        <property type="term" value="F:malate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006107">
        <property type="term" value="P:oxaloacetate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1560">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005000">
        <property type="entry name" value="Aldolase/citrate-lyase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053484">
        <property type="entry name" value="MS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059088">
        <property type="entry name" value="MSH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041315">
        <property type="entry name" value="malate_syn_AceB_Halo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32308:SF0">
        <property type="entry name" value="HPCH_HPAI ALDOLASE_CITRATE LYASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32308">
        <property type="entry name" value="LYASE BETA SUBUNIT, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G13030)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03328">
        <property type="entry name" value="HpcH_HpaI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25918">
        <property type="entry name" value="MSH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HpcH/HpaI aldolase/citrate lyase" evidence="4">
        <location>
            <begin position="40"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="Malate synthase H C-terminal" evidence="5">
        <location>
            <begin position="281"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25918"/>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="436" mass="48407" checksum="1F9D86B27070E065" modified="2017-05-10" version="1">MTLDKRTHDRTFVRTFFTTPTAEKGVEDSAKKLRSAIELRGMQAPDVWVPDNEDATAPNMRDEGAENIAEVVAESGADFPGEIHPRVVWHRDSPETRYQGFQHMLEIADPENGAIEHIDGFVIPEVGDIDDWKKADEFITIVENEYGLEEGSIAMSVIIESGSAELAMGKLREEMGKADNNLERLFLLVDGEVDYTKDMRAITPTGGLPEWKELRHNTSRAASAAGLISVDGPYDDIRDVEGYHDRITANNAMGMLGIWSLTPGQVVEANKSPLPPATGYWLIDADGREVELESEDGVEVYSGDRIELEEEDGGYELEVGSTEIFCESEEELADELLDLVDYVPSMDDIVDSMEEFEAAKEAGKGAIAMTRAATIEIDGVQVDVSSDRMWDEATYQALMTPVKLFQDVYENRPDQHDDLADLYSEDVVERAIEVGN</sequence>
</entry>
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        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
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            <dbReference type="PubMed" id="16169924"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="gene ID" value="NP_4536A"/>
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    <feature type="transmembrane region" description="Helical" evidence="1">
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            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI50359.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="74" mass="8415" checksum="853D4CDFADFBFB31" modified="2017-05-10" version="1">MVSTAERYGWAVLFAVLLALAIPWFLWGDSRTALGLPLWLWWHVGWLAASSVVFWVFTKRAWGIGIVEPAGEKR</sequence>
</entry>
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        <lineage>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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                <person name="Pfeiffer F."/>
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                <person name="Rodewald K."/>
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            <dbReference type="PubMed" id="16169924"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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    <dbReference type="STRING" id="348780.NP_4548A"/>
    <dbReference type="EnsemblBacteria" id="CAI50365">
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    </dbReference>
    <dbReference type="GeneID" id="3702585"/>
    <dbReference type="KEGG" id="nph:NP_4548A"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="75"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50365.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="134" mass="14409" checksum="578CBCA63429CB7E" modified="2017-05-10" version="1">MEDIFVGRIMSSPVATTQPETPIQEAAQTMLDQSINSLIIVGEDGHPEGILTSTDLVHVVADQTPIDDSRVEEYMNQVDTVTTANTSIQEAADTMMARGTHHLPVVEDEAGLIGILTTTDLTAYLSDNWAPSPS</sequence>
</entry>
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    <accession>A0A1U7EYV0</accession>
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            <fullName evidence="4">DUF4129 domain protein</fullName>
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    <organism evidence="4 5">
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
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            <taxon>Halobacteriales</taxon>
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    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
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            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50412.1"/>
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    <dbReference type="STRING" id="348780.NP_4642A"/>
    <dbReference type="EnsemblBacteria" id="CAI50412">
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        <property type="gene ID" value="NP_4642A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702130"/>
    <dbReference type="KEGG" id="nph:NP_4642A"/>
    <dbReference type="eggNOG" id="arCOG02085">
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    </dbReference>
    <dbReference type="eggNOG" id="arCOG02170">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG07873">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015922_0_0_2"/>
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        <property type="entry name" value="TgpA-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13559">
        <property type="entry name" value="DUF4129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="Protein-glutamine gamma-glutamyltransferase-like C-terminal" evidence="3">
        <location>
            <begin position="722"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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        </location>
    </feature>
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        <location>
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    </feature>
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        </location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
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            <end position="166"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
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            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="251"/>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
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        </location>
    </feature>
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        <location>
            <begin position="852"/>
            <end position="864"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF13559"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI50412.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="864" mass="90560" checksum="0734DCDEE42A4F98" modified="2017-05-10" version="1"> </sequence>
</entry>
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    <accession>A0A1U7EYX2</accession>
    <name>A0A1U7EYX2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">PQQ repeat protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="4" type="ordered locus">NP_4750A</name>
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    <organism evidence="4 5">
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    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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            <authorList>
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                <person name="Rodewald K."/>
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                <person name="Tittor J."/>
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            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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    <dbReference type="EnsemblBacteria" id="CAI50466">
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        <property type="gene ID" value="NP_4750A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702166"/>
    <dbReference type="KEGG" id="nph:NP_4750A"/>
    <dbReference type="eggNOG" id="arCOG02556">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027480_4_2_2"/>
    <dbReference type="OrthoDB" id="136681at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.480">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002372">
        <property type="entry name" value="PQQ_rpt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34512">
        <property type="entry name" value="CELL SURFACE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34512:SF30">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN ASSEMBLY FACTOR BAMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01011">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13360">
        <property type="entry name" value="PQQ_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyrrolo-quinoline quinone repeat" evidence="3">
        <location>
            <begin position="83"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrrolo-quinoline quinone repeat" evidence="2">
        <location>
            <begin position="169"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrrolo-quinoline quinone repeat" evidence="3">
        <location>
            <begin position="266"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="397" mass="40870" checksum="9918AB177534E06D" modified="2017-05-10" version="1">MRWTRRRVVAAIGAATTAAVAGCSDTDEDAENGTPAGQEHEQTPTPEQTPEPASAVPMVGYDATRTAAPPGVAGPAGAVEADWTFELDDRTTAAPVIADGTVYIGGDDLTVYAIDADDGTERWQYSTNGDVTAAAATDGDRVYVGSSDGNLYALADGDDEWVFETGDGIDSAPAVVDGTVYVGSGDRTVYAVDADSGQEQWHFDADDSVTGAPAVVGDTVYVGSRDSNVYAIAADDGTERWTFRTEGAGSVSPAVADGTVYVGCWDGRLYAIDAAEGEERWQLSLDGRVQASPAVADGTVYAASTGGDVVAATTDGEALWRTNTNSRIESAPTVAGGVVYLGSGDRNVYAFDAANDGEALWRVDVEGAVHAAPAVLDGTVYAGTYGGDVVALRNESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ09</accession>
    <name>A0A1U7EZ09_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Homolog to sodium/calcium antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tp44</name>
        <name evidence="7" type="ordered locus">NP_4778A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324092.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ09"/>
    <dbReference type="STRING" id="348780.NP_4778A"/>
    <dbReference type="EnsemblBacteria" id="CAI50480">
        <property type="protein sequence ID" value="CAI50480"/>
        <property type="gene ID" value="NP_4778A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703322"/>
    <dbReference type="KEGG" id="nph:NP_4778A"/>
    <dbReference type="eggNOG" id="arCOG02881">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007948_0_0_2"/>
    <dbReference type="OrthoDB" id="204563at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008273">
        <property type="term" value="F:calcium, potassium:sodium antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004481">
        <property type="entry name" value="K/Na/Ca-exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846:SF8">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YRBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846">
        <property type="entry name" value="SODIUM/POTASSIUM/CALCIUM EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="74"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="219"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="253"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="281"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="312"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="6">
        <location>
            <begin position="8"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="6">
        <location>
            <begin position="185"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI50480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="336" mass="34809" checksum="304663E161458204" modified="2017-05-10" version="1">MTSIGVLLGIGAVATAVVWVGSSALERASGRLARHYGLPRLVQGTILAALGSSAPEIASTVIATIRYGEFELGVGVVVGSAVFNILVIPALSALLADGALDTGREVVYKEAQFYMLSVAALFLVFALAVIYFPAEGDGLQGNVTRPLAVSLLLLYLLYIFVQYLDVSESNAKTTRLERRRLAIEWGLLVAGVGLIIAGVEGLVVAAIGLGEAFGTPSFLWGLTVVAAATSLPDALISVAAARIDEPAVSLGNVLGSNIFDLLVAIPAGVLLGGAVAVSFTAAVPMMAFLVVATVVLFAFARTGMEISNAEAWAMLLLYGSFIAWLLLESIGVIDIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ48</accession>
    <name>A0A1U7EZ48_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CopG domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4934A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324170.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ48"/>
    <dbReference type="STRING" id="348780.NP_4934A"/>
    <dbReference type="EnsemblBacteria" id="CAI50558">
        <property type="protein sequence ID" value="CAI50558"/>
        <property type="gene ID" value="NP_4934A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702205"/>
    <dbReference type="KEGG" id="nph:NP_4934A"/>
    <dbReference type="eggNOG" id="arCOG04566">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055067_0_0_2"/>
    <dbReference type="OrthoDB" id="178000at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="135"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="267" mass="29658" checksum="5BA5E6ACA3520A5C" modified="2017-05-10" version="1">MATEPAGGAEFSVSLPPSLAEWLDERAATLGMDREELLVQLVGSYRTAAEIGDEETKELMQSLDIEAVVDEAVADRLEAADDLGEIEGRLEAVETGLADNVEDLRSRVLQLRDLVQDRAHADHGHEEFDQLATRTEDLAGELETVRRDIEDLSQELTTVDDRFEDVEGKLDRLARVVLALRREIESISTTDAEILAHLRQRANRNGTTDAVCASCGERVSIGLLGEPLCPYCDVEFRDIEYADGRLRWFSTPRLVGPDTPKAEHDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ53</accession>
    <name>A0A1U7EZ53_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Probable tRNA pseudouridine synthase B</fullName>
            <ecNumber evidence="3">5.4.99.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">tRNA pseudouridine(55) synthase</fullName>
            <shortName evidence="3">Psi55 synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">tRNA pseudouridylate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">tRNA-uridine isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cbf5</name>
        <name evidence="3 5" type="synonym">truB</name>
        <name evidence="5" type="ordered locus">NP_4916A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>uridine(55) in tRNA = pseudouridine(55) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42532"/>
            <dbReference type="Rhea" id="RHEA-COMP:10101"/>
            <dbReference type="Rhea" id="RHEA-COMP:10102"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the pseudouridine synthase TruB family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.25" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324161.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ53"/>
    <dbReference type="STRING" id="348780.NP_4916A"/>
    <dbReference type="EnsemblBacteria" id="CAI50549">
        <property type="protein sequence ID" value="CAI50549"/>
        <property type="gene ID" value="NP_4916A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703421"/>
    <dbReference type="KEGG" id="nph:NP_4916A"/>
    <dbReference type="eggNOG" id="arCOG00987">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032087_3_0_2"/>
    <dbReference type="OrthoDB" id="35866at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160148">
        <property type="term" value="F:tRNA pseudouridine(55) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000495">
        <property type="term" value="P:box H/ACA sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031118">
        <property type="term" value="P:rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031120">
        <property type="term" value="P:snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01081">
        <property type="entry name" value="TruB_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012960">
        <property type="entry name" value="Dyskerin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002501">
        <property type="entry name" value="PsdUridine_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004802">
        <property type="entry name" value="tRNA_PsdUridine_synth_B_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026326">
        <property type="entry name" value="TruB_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032819">
        <property type="entry name" value="TruB_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00425">
        <property type="entry name" value="CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003280">
        <property type="entry name" value="PRK04270.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127">
        <property type="entry name" value="CENTROMERE/MICROTUBULE BINDING PROTEIN CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127:SF0">
        <property type="entry name" value="H_ACA RIBONUCLEOPROTEIN COMPLEX SUBUNIT DKC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08068">
        <property type="entry name" value="DKCLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16198">
        <property type="entry name" value="TruB_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01509">
        <property type="entry name" value="TruB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01136">
        <property type="entry name" value="DKCLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Dyskerin-like" evidence="4">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI50549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="292" mass="31139" checksum="D3E2BA39973BC084" modified="2017-05-10" version="1">MRERGPPADRDPETLLEFGVINLDKPPGPSAHQVAAWVRDMAGVEQAAHAGTLDPKVTGCLPVLTGTATRAAQVFDESRKGYVAVLELHGTAPSDLESTVAEFEGPLYQKPPRKSAVARRLRTRTVHRLDVLESEARRVLLSVECESGTYIRKLCHDIGLAVGTGGHMGPLRRTKTGGFDDRTLVTFEDFADGLAFWRDHDDPELLCDVVAPAERALEGLPRLTVAFSAASEIANGAPVYAPGVLAHELDGADEGSLVACYTPDGAAVCLGWLVGDPDAADGTVAELERVLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZC0</accession>
    <name>A0A1U7EZC0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcription initiation factor IIB</fullName>
            <shortName evidence="8">TFIIB</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">tfbA7</name>
        <name evidence="8" type="synonym">tfb</name>
        <name evidence="11" type="ordered locus">NP_5096A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre-initiation complex (DNA-TBP-TFIIB).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the TFIIB family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324249.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZC0"/>
    <dbReference type="STRING" id="348780.NP_5096A"/>
    <dbReference type="EnsemblBacteria" id="CAI50639">
        <property type="protein sequence ID" value="CAI50639"/>
        <property type="gene ID" value="NP_5096A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703239"/>
    <dbReference type="KEGG" id="nph:NP_5096A"/>
    <dbReference type="eggNOG" id="arCOG01981">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043736_0_0_2"/>
    <dbReference type="OrthoDB" id="7429at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00383">
        <property type="entry name" value="TF2B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023484">
        <property type="entry name" value="TFIIB_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF13">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="Zn_Ribbon_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="1" evidence="8">
        <location>
            <begin position="112"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="10">
        <location>
            <begin position="112"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="repeat" description="2" evidence="8">
        <location>
            <begin position="206"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="10">
        <location>
            <begin position="206"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="41"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI50639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="287" mass="31462" checksum="66D97FBE630FC219" modified="2017-05-10" version="1">MYTATRKACPECDGRLRSTDTETVCDECGLVVAEDELDRGPEWRSFSDDETNPERCGAPLTRSRHDRGLSTEIGRSTRLKGRKRRRVARMRRQHNRAQVSSKRERNQMHGFIEVRRLIGQLGLPEALQERACVLFESAQNEDLLQGRCIEGFAAAAVYVVCRTASVSRTLDEIVAAASATRDELTVAYDALNRELGIPTGPIAPTEYLARFASKLGLPSAVERTARELATDAEESGLAVGRDPSGVAAACLYTAAEEHDHDVTQAEAGDVAGVSAVTVRSTYHDLRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZC3</accession>
    <name>A0A1U7EZC3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">SSSF family transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">NP_5136A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324269.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZC3"/>
    <dbReference type="STRING" id="348780.NP_5136A"/>
    <dbReference type="EnsemblBacteria" id="CAI50659">
        <property type="protein sequence ID" value="CAI50659"/>
        <property type="gene ID" value="NP_5136A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702267"/>
    <dbReference type="KEGG" id="nph:NP_5136A"/>
    <dbReference type="eggNOG" id="arCOG01317">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018808_8_2_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11480">
        <property type="entry name" value="SLC5sbd_u4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019899">
        <property type="entry name" value="Na/solute_symporter_VC_2705"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050277">
        <property type="entry name" value="Sodium:Solute_Symporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03648">
        <property type="entry name" value="Na_symport_lg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48086:SF5">
        <property type="entry name" value="NA(+):SOLUTE SYMPORTER (SSF FAMILY)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48086">
        <property type="entry name" value="SODIUM/PROLINE SYMPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="188"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="269"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="306"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="365"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="413"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="437"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="469"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="507"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI50659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="577" mass="61429" checksum="E1B16D207E28108A" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZH1</accession>
    <name>A0A1U7EZH1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7311 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5202A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324302.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZH1"/>
    <dbReference type="STRING" id="348780.NP_5202A"/>
    <dbReference type="EnsemblBacteria" id="CAI50692">
        <property type="protein sequence ID" value="CAI50692"/>
        <property type="gene ID" value="NP_5202A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702292"/>
    <dbReference type="KEGG" id="nph:NP_5202A"/>
    <dbReference type="eggNOG" id="arCOG10216">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1745600_0_0_2"/>
    <dbReference type="OrthoDB" id="384457at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055735">
        <property type="entry name" value="DUF7311"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23993">
        <property type="entry name" value="DUF7311"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7311" evidence="1">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="149" mass="15085" checksum="FB3F547702839944" modified="2017-05-10" version="1">MIRVVLAAVLATALVGVSLPAAERAEADRNTALATGELAAVAETAEELAAENDPVTSGTGAASTDIIVDSPSGVFAAGGSLIIDSKLRWVPKDGPEQSVETAVRLNTTEPIQITGQSRLRLSLITGSDGEPTVSIERVHGGNGRAAAEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZH5</accession>
    <name>A0A1U7EZH5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5 6">RNA 3'-terminal phosphate cyclase</fullName>
            <shortName evidence="5">RNA cyclase</shortName>
            <shortName evidence="5">RNA-3'-phosphate cyclase</shortName>
            <ecNumber evidence="5 6">6.5.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 9" type="primary">rtcA</name>
        <name evidence="9" type="synonym">tpc</name>
        <name evidence="9" type="ordered locus">NP_5244A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 3'-end 3'-phospho-ribonucleotide-RNA + ATP = a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23976"/>
            <dbReference type="Rhea" id="RHEA-COMP:10463"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:83062"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.5.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.4" evidence="5 6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324323.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZH5"/>
    <dbReference type="STRING" id="348780.NP_5244A"/>
    <dbReference type="EnsemblBacteria" id="CAI50713">
        <property type="protein sequence ID" value="CAI50713"/>
        <property type="gene ID" value="NP_5244A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703356"/>
    <dbReference type="KEGG" id="nph:NP_5244A"/>
    <dbReference type="eggNOG" id="arCOG04125">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027882_0_0_2"/>
    <dbReference type="OrthoDB" id="7994at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003963">
        <property type="term" value="F:RNA-3'-phosphate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00200">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013791">
        <property type="entry name" value="RNA3'-term_phos_cycl_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023797">
        <property type="entry name" value="RNA3'_phos_cyclase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037136">
        <property type="entry name" value="RNA3'_phos_cyclase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000228">
        <property type="entry name" value="RNA3'_term_phos_cyc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017770">
        <property type="entry name" value="RNA3'_term_phos_cyc_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036553">
        <property type="entry name" value="RPTC_insert"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003246">
        <property type="entry name" value="PRK04204.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03399">
        <property type="entry name" value="RNA_3prim_cycl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096">
        <property type="entry name" value="RNA 3' TERMINAL PHOSPHATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096:SF0">
        <property type="entry name" value="RNA 3'-TERMINAL PHOSPHATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01137">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05189">
        <property type="entry name" value="RTC_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005378">
        <property type="entry name" value="RNA3'_term_phos_cycl_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="EPT/RTPC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52913">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase, RPTC, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase" evidence="7">
        <location>
            <begin position="9"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase insert" evidence="8">
        <location>
            <begin position="177"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="5">
        <location>
            <position position="305"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="NCBIfam" id="TIGR03399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI50713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="338" mass="34687" checksum="94180F9876B42B4E" modified="2017-05-10" version="1">MHEIDGSAGGGQMVRSAVSLSALSGEAVRVENVRGDRSTPGMRPQHVAAVEAAAALTDASVEGAEQGAETLVFDPGSVSATDVSVDVGTAGSIALVFDTVLPLATALSTPASVTVTGGTDVEWAPPLAYTRRVKLPVLAAHGLDAAIDVEQRGFYPAGGGEATLSLSPSSLSPLALTERGPLRRLSVVSVADDSLAEADVAERQAAAAVENIDVGAPASETVDYDDAACAGSSVTVVGVYEHTRVGFSALGEKGTPAETVAAEAAADFQRFHESAAAVDERLADQLLVYQALAGGTVRAPSATAHVETNRQVIEPFGYEFEFTRLDDTVRIERAAVDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZI3</accession>
    <name>A0A1U7EZI3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5290A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324345.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZI3"/>
    <dbReference type="STRING" id="348780.NP_5290A"/>
    <dbReference type="EnsemblBacteria" id="CAI50736">
        <property type="protein sequence ID" value="CAI50736"/>
        <property type="gene ID" value="NP_5290A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702322"/>
    <dbReference type="KEGG" id="nph:NP_5290A"/>
    <dbReference type="HOGENOM" id="CLU_2712949_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="72" mass="7126" checksum="E763F33469D7D53B" modified="2017-05-10" version="1">MSEPNSTYGKAGVGVLLLAAVVWSAAVVLAESGAAPDATTQGIGYVGLALLAVGMVAHVLLILSAYGPDDLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZI4</accession>
    <name>A0A1U7EZI4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Alpha/beta hydrolase fold protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_5282A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324341.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZI4"/>
    <dbReference type="STRING" id="348780.NP_5282A"/>
    <dbReference type="EnsemblBacteria" id="CAI50732">
        <property type="protein sequence ID" value="CAI50732"/>
        <property type="gene ID" value="NP_5282A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702318"/>
    <dbReference type="KEGG" id="nph:NP_5282A"/>
    <dbReference type="eggNOG" id="arCOG01646">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_367932_0_0_2"/>
    <dbReference type="OrthoDB" id="227610at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050955">
        <property type="entry name" value="Plant_Biomass_Hydrol_Est"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43037:SF5">
        <property type="entry name" value="FERULOYL ESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43037">
        <property type="entry name" value="UNNAMED PRODUCT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49344">
        <property type="entry name" value="CBD9-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="4">
        <location>
            <begin position="451"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="685"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI50732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="785" mass="86098" checksum="74E66296CD6BD66A" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZI5</accession>
    <name>A0A1U7EZI5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">NP_5274A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324337.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZI5"/>
    <dbReference type="STRING" id="348780.NP_5274A"/>
    <dbReference type="EnsemblBacteria" id="CAI50728">
        <property type="protein sequence ID" value="CAI50728"/>
        <property type="gene ID" value="NP_5274A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702315"/>
    <dbReference type="KEGG" id="nph:NP_5274A"/>
    <dbReference type="eggNOG" id="arCOG02369">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_58_2"/>
    <dbReference type="OrthoDB" id="342253at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00075">
        <property type="entry name" value="HATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013656">
        <property type="entry name" value="PAS_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050736">
        <property type="entry name" value="Sensor_HK_Regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43711:SF1">
        <property type="entry name" value="HISTIDINE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43711">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08448">
        <property type="entry name" value="PAS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="PAS" evidence="8">
        <location>
            <begin position="11"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="314"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI50728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="508" mass="55699" checksum="F2F0288A2C643869" modified="2017-05-10" version="1">MASPVTPDEGLVQAYERLVADAPFGVLTIDTDSTIRYANSAVTDLLGYDSEALAGAPLETIIPDRLRDSHTEAFEAYLETGERHLDWNNVELPALHRDGHEVRVSIAFRETPTDEETLFTGVFTDLSGGHRLRGAPQETIGTLHELYVIASDATLSFEQRRESILDLGCRYLDLEYGFVTEITSDEQVIVASVGDHDLLQPGERCPIEESYCRETISEDGFLAIADAAAEGFEDDPAYERFGLGSYIGGKLLVAGELFGTLCFASTAPRDRTFTDGERTFVELASRWLGYELQQRHVQRQLERQNDRLEEFASHASHDLRNPLSAAAGRLELAIEQHGDDEDLLAVKRSLEDADARIDEMLEFARAGNTVTDPKPVSLSEAAAAAWETLDTEGATLAVDDEVRLRGDRDRIERLFENLFRNAVEHAGEDATVRLGALPTPGFYVADDGPGIPAGDRETVFKSGFTTDAGGSGYGLSIVEEIATAHGWSVEACEDGDGARFEFTVTDRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZL1</accession>
    <name>A0A1U7EZL1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Small CPxCG-related zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_5316A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324358.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZL1"/>
    <dbReference type="STRING" id="348780.NP_5316A"/>
    <dbReference type="EnsemblBacteria" id="CAI50749">
        <property type="protein sequence ID" value="CAI50749"/>
        <property type="gene ID" value="NP_5316A"/>
    </dbReference>
    <dbReference type="GeneID" id="54763335"/>
    <dbReference type="KEGG" id="nph:NP_5316A"/>
    <dbReference type="eggNOG" id="arCOG04795">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3075224_0_0_2"/>
    <dbReference type="OrthoDB" id="189643at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055981">
        <property type="entry name" value="DUF7559"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24440">
        <property type="entry name" value="DUF7559"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="52" mass="5860" checksum="405F3E540CE292A0" modified="2017-05-10" version="1">MPKTLEVVCNREACDLDMMELHYRYSMPDDTGVSDFVCPYCGEANGLEAIEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZL6</accession>
    <name>A0A1U7EZL6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">STAS/SEC14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_5334A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324367.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZL6"/>
    <dbReference type="EnsemblBacteria" id="CAI50758">
        <property type="protein sequence ID" value="CAI50758"/>
        <property type="gene ID" value="NP_5334A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702337"/>
    <dbReference type="KEGG" id="nph:NP_5334A"/>
    <dbReference type="eggNOG" id="arCOG06336">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1998706_0_0_2"/>
    <dbReference type="OrthoDB" id="218982at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="124" mass="14008" checksum="CB82A8F1A9F551B5" modified="2017-05-10" version="1">MSEKQRGDGWTVEIDDGVMIWEFLPGMELSAFREDAYPVYEDLLDQYNVDGMVTVVKLDDAFNEQVFEVWEKSAQRAEQAGIERWAVVADGIKALSLRGKVETGGLDTLTTEDRTEAVEWARDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZM5</accession>
    <name>A0A1U7EZM5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF8053 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_5356A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324377.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZM5"/>
    <dbReference type="STRING" id="348780.NP_5356A"/>
    <dbReference type="EnsemblBacteria" id="CAI50769">
        <property type="protein sequence ID" value="CAI50769"/>
        <property type="gene ID" value="NP_5356A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702348"/>
    <dbReference type="KEGG" id="nph:NP_5356A"/>
    <dbReference type="eggNOG" id="arCOG07539">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_206273_0_0_2"/>
    <dbReference type="OrthoDB" id="201619at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058366">
        <property type="entry name" value="DUF8053"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26227">
        <property type="entry name" value="DUF8053"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8053" evidence="2">
        <location>
            <begin position="3"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="59" mass="6630" checksum="B6F8CF31350B1095" modified="2017-05-10" version="1">MPIRKLRQLDDDSAGVTLPKDDLRENGLLGDDGQIEDDQHVVIEQVDDGEWTVRTVDVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZN4</accession>
    <name>A0A1U7EZN4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_5376A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324387.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZN4"/>
    <dbReference type="STRING" id="348780.NP_5376A"/>
    <dbReference type="EnsemblBacteria" id="CAI50779">
        <property type="protein sequence ID" value="CAI50779"/>
        <property type="gene ID" value="NP_5376A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702357"/>
    <dbReference type="KEGG" id="nph:NP_5376A"/>
    <dbReference type="eggNOG" id="arCOG07569">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135997_0_0_2"/>
    <dbReference type="OrthoDB" id="256252at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055944">
        <property type="entry name" value="DUF7522"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24366">
        <property type="entry name" value="DUF7522"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="130" mass="14942" checksum="955EFF49F1EE21BB" modified="2017-05-10" version="1">MRRVSEDQPNQQLAEELQSICRAAVGDELRSITYFTDEDVEQLYLRSDLDRTADLFGFAELERNGFEADEKYRNSQLGDYRATIRLFDNGYLTRVTLNDHGVWVTTDSMSIDRFEELSESLRTALSSADG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZR2</accession>
    <name>A0A1U7EZR2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Small CPxCG-related zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_5331B</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CCI69588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZR2"/>
    <dbReference type="EnsemblBacteria" id="CCI69588">
        <property type="protein sequence ID" value="CCI69588"/>
        <property type="gene ID" value="NP_5331B"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_5331B"/>
    <dbReference type="HOGENOM" id="CLU_3371396_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCI69588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="34" mass="3571" checksum="EC3E2970DFDD46E3" modified="2017-05-10" version="1">MTCPVCGESSTKIYRCTSCGADLVETGTEGREDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILR9</accession>
    <name>Q3ILR9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3248A</name>
        <name evidence="2" type="ordered locus">NP_7072A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>PL23</name>
    </geneLocation>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
            <strain evidence="2">Gabara</strain>
            <plasmid evidence="2">PL23</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CR936259">
        <property type="protein sequence ID" value="CAI50952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323337.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILR9"/>
    <dbReference type="STRING" id="348780.NP_3248A"/>
    <dbReference type="EnsemblBacteria" id="CAI49715">
        <property type="protein sequence ID" value="CAI49715"/>
        <property type="gene ID" value="NP_3248A"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI50952">
        <property type="protein sequence ID" value="CAI50952"/>
        <property type="gene ID" value="NP_7072A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703495"/>
    <dbReference type="KEGG" id="nph:NP_3248A"/>
    <dbReference type="KEGG" id="nph:NP_7072A"/>
    <dbReference type="HOGENOM" id="CLU_2461860_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="88" mass="9877" checksum="6136A1BE178602AD" modified="2005-11-08" version="1">MTRGIAYRYAEKIRQEAIDIDQQRLEELIDEAIEEADLTVDYRARDEIQETVDRAIDESIDGDLTGILEAALAAYSDQATVGGREVSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILZ1</accession>
    <name>Q3ILZ1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC-type transport system ATP-binding protein (Probable substrate branched-chain amino acids)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">livG2</name>
        <name evidence="5" type="synonym">abc06a2</name>
        <name evidence="5" type="ordered locus">NP_6196A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5 6">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324476.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILZ1"/>
    <dbReference type="EnsemblBacteria" id="CAI50878">
        <property type="protein sequence ID" value="CAI50878"/>
        <property type="gene ID" value="NP_6196A"/>
    </dbReference>
    <dbReference type="GeneID" id="69102752"/>
    <dbReference type="KEGG" id="nph:NP_6196A"/>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03219">
        <property type="entry name" value="ABC_Mj1267_LivG_branched"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051120">
        <property type="entry name" value="ABC_AA/LPS_Transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032823">
        <property type="entry name" value="BCA_ABC_TP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45772:SF9">
        <property type="entry name" value="CONSERVED COMPONENT OF ABC TRANSPORTER FOR NATURAL AMINO ACIDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45772">
        <property type="entry name" value="CONSERVED COMPONENT OF ABC TRANSPORTER FOR NATURAL AMINO ACIDS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12399">
        <property type="entry name" value="BCA_ABC_TP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="6"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI50878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="245" mass="27076" checksum="65E34BF5179D4CB2" modified="2005-11-08" version="1">MSEPIIETENLTKRFGALVANDSISISVDRGEIRGVIGPNGSGKTTFFNNLTGFYTPDEGAVRFNGVDITGWEPHRIAREGMARTFQIVSPFRNMTVTENLLAVDAPDSVDNKRERAQEILEFLEIDHIADNDAEGMSGGQQKLLELGRVLMLDPDCILLDEPSAGVNPALEKRILEHIQELNDQGTTFVMVEHDMDVMRTIADTVTVLDQGKIIAEGSFDDVTGNDRVREAYLGREVDLEEVLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILZ9</accession>
    <name>Q3ILZ9_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">glutaryl-CoA dehydrogenase (ETF)</fullName>
            <ecNumber evidence="9">1.3.8.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">acd8</name>
        <name evidence="15" type="ordered locus">NP_6180A</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="15 16">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>glutaryl-CoA + oxidized [electron-transfer flavoprotein] + 2 H(+) = (2E)-butenoyl-CoA + reduced [electron-transfer flavoprotein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:13389"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57332"/>
            <dbReference type="ChEBI" id="CHEBI:57378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="EC" id="1.3.8.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid metabolism; lysine degradation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid metabolism; tryptophan metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.6" evidence="9"/>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324468.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILZ9"/>
    <dbReference type="EnsemblBacteria" id="CAI50870">
        <property type="protein sequence ID" value="CAI50870"/>
        <property type="gene ID" value="NP_6180A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694679"/>
    <dbReference type="KEGG" id="nph:NP_6180A"/>
    <dbReference type="HOGENOM" id="CLU_018204_8_0_2"/>
    <dbReference type="OrthoDB" id="275197at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000062">
        <property type="term" value="F:fatty-acyl-CoA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004361">
        <property type="term" value="F:glutaryl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046949">
        <property type="term" value="P:fatty-acyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000002">
        <property type="entry name" value="Acyl-CoA dehydrogenase fadE12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052033">
        <property type="entry name" value="Glutaryl-CoA_DH_mitochondrial"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42807">
        <property type="entry name" value="GLUTARYL-COA DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42807:SF1">
        <property type="entry name" value="GLUTARYL-COA DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0274">FAD</keyword>
    <keyword evidence="3 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6 11 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-0614">Plasmid</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="14">
        <location>
            <begin position="13"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="13">
        <location>
            <begin position="127"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="12">
        <location>
            <begin position="234"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAI50870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="387" mass="42625" checksum="B9A008B1AD70ADF7" modified="2005-11-08" version="1">MLDYLGLESDLDEEERLIQETARDFVEDNVAPDIGQHWIDGTFPTDLIPKMGELGFYAPNLEGYGSPNVSETAYGLLMQELEACDSGLRSMASVQGALVMYPIHAYGSEAQKEEWLPALGQGEAVGCFGLTEPEHGSNPTAMETQAEKDGDEYVLNGSKTWITNSPIADVAVVWARDTSADDTPVRGFLVETDRDGVTTNKIDEKLSLRASITGEIGLQNVRVPETNVLPEVEGMKGPLSCLTQARYGIAWGAIGAARDCFETAREYATDREQFGTPIGQFQLQQDKLAEMATQISLAQLLAHRLAELKERGDMRPQHVSMSKRNNVRMARNQSRIAREMLGGNGITADYSPMRHMANLETVYTYEGTHDIHTLILGEDLTGLQAYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="124" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM43</accession>
    <name>Q3IM43_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Sensor box histidine kinase</fullName>
            <ecNumber evidence="10">2.7.13.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">NP_6080A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10 11">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.13.3" evidence="10"/>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324424.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM43"/>
    <dbReference type="EnsemblBacteria" id="CAI50820">
        <property type="protein sequence ID" value="CAI50820"/>
        <property type="gene ID" value="NP_6080A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694705"/>
    <dbReference type="KEGG" id="nph:NP_6080A"/>
    <dbReference type="HOGENOM" id="CLU_000445_114_58_2"/>
    <dbReference type="OrthoDB" id="165911at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00075">
        <property type="entry name" value="HATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43065">
        <property type="entry name" value="SENSOR HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="PAS" evidence="8">
        <location>
            <begin position="13"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="8">
        <location>
            <begin position="123"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="8">
        <location>
            <begin position="242"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="9">
        <location>
            <begin position="313"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="370"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI50820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="582" mass="65283" checksum="2DFBC074F8324721" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM75</accession>
    <name>Q3IM75_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_6008A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324392.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM75"/>
    <dbReference type="EnsemblBacteria" id="CAI50784">
        <property type="protein sequence ID" value="CAI50784"/>
        <property type="gene ID" value="NP_6008A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694578"/>
    <dbReference type="KEGG" id="nph:NP_6008A"/>
    <dbReference type="HOGENOM" id="CLU_1109538_0_0_2"/>
    <dbReference type="OrthoDB" id="261502at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058394">
        <property type="entry name" value="DUF8081"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059118">
        <property type="entry name" value="PDDEXK_dom_halobact"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26297">
        <property type="entry name" value="DUF8081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26295">
        <property type="entry name" value="PDDEXK_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8081" evidence="2">
        <location>
            <begin position="43"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="PD-(D/E)XK nuclease-like" evidence="1">
        <location>
            <begin position="135"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="299" mass="33273" checksum="422B2E7F2AAF4F29" modified="2005-11-08" version="1">MISIQPLRSFRPVVVTSSNDGIAIPTKIILGAVVMPMESGDSFRVVIKKSALEANDAARRLCDKEGDCVAFDSEKEAEEMAAELTRDATGEIKIQQAAPNDPADVDAYLVHFPKQRKRTPDGSLQKGWTFDTTANQYGAIGETLITAPGTPAVKHFVKQDLGPLPSDEVGSRIRTEVKTDKEEFDWETLRSAVANDWINWTPDCEIRVLIGYPPAQVHQYFCEIKSGDASFERNQQGAMDAASKNVDVLKIRVTLEGLPNEYSVKFRRVGDSSPDVNWENLLERVTPFCRDQTDLSDFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IML1</accession>
    <name>Q3IML1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PfpI family protease</fullName>
            <ecNumber evidence="2">3.4.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5112A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.4.-.-" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324257.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IML1"/>
    <dbReference type="STRING" id="348780.NP_5112A"/>
    <dbReference type="EnsemblBacteria" id="CAI50647">
        <property type="protein sequence ID" value="CAI50647"/>
        <property type="gene ID" value="NP_5112A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702260"/>
    <dbReference type="KEGG" id="nph:NP_5112A"/>
    <dbReference type="eggNOG" id="arCOG00769">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_44_1_2"/>
    <dbReference type="OrthoDB" id="8348at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03139">
        <property type="entry name" value="GATase1_PfpI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002818">
        <property type="entry name" value="DJ-1/PfpI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052158">
        <property type="entry name" value="INH-QAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43130">
        <property type="entry name" value="ARAC-FAMILY TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43130:SF3">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR RV1931C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01965">
        <property type="entry name" value="DJ-1_PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DJ-1/PfpI" evidence="1">
        <location>
            <begin position="3"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="193" mass="20182" checksum="222900D6D26FF0C2" modified="2005-11-08" version="1">MNVDILLFDGFDELDAVGPYEVLRLAGRHGGDIAARLVTVEGVAAVEARNGLRVRTDGHPDDNADLLLVPGGGWNDSDAPGVRREHERGVVPEFIADRHADGVTVASVCTGAMLLAKAGLLDGRPATTHHTAHEDLSDAGANLRTDRFVDDGDVLTAGGITAGFDLALAIVEDHCGADIAAAVATELEYERAI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-28" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IPH3</accession>
    <name>GATE_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamyl-tRNA(Gln) amidotransferase subunit E</fullName>
            <shortName evidence="1">Glu-ADT subunit E</shortName>
            <ecNumber evidence="1">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gatE</name>
        <name type="ordered locus">NP_3774A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The GatDE system is specific for glutamate and does not act on aspartate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of GatD and GatE.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GatB/GatE family. GatE subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323595.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IPH3"/>
    <dbReference type="SMR" id="Q3IPH3"/>
    <dbReference type="STRING" id="348780.NP_3774A"/>
    <dbReference type="EnsemblBacteria" id="CAI49978">
        <property type="protein sequence ID" value="CAI49978"/>
        <property type="gene ID" value="NP_3774A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702739"/>
    <dbReference type="KEGG" id="nph:NP_3774A"/>
    <dbReference type="eggNOG" id="arCOG01719">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030702_0_0_2"/>
    <dbReference type="OrthoDB" id="7316at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004812">
        <property type="term" value="F:aminoacyl-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070681">
        <property type="term" value="P:glutaminyl-tRNAGln biosynthesis via transamidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.410:FF:000003">
        <property type="entry name" value="Glutamyl-tRNA(Gln) amidotransferase subunit E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.30">
        <property type="entry name" value="GAD-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.380">
        <property type="entry name" value="GatB domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00588">
        <property type="entry name" value="GatE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017959">
        <property type="entry name" value="Asn/Gln-tRNA_amidoTrfase_suB/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006075">
        <property type="entry name" value="Asn/Gln-tRNA_Trfase_suB/E_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018027">
        <property type="entry name" value="Asn/Gln_amidotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003789">
        <property type="entry name" value="Asn/Gln_tRNA_amidoTrase-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004115">
        <property type="entry name" value="GAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029351">
        <property type="entry name" value="GAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042114">
        <property type="entry name" value="GatB_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023168">
        <property type="entry name" value="GatB_Yqey_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004414">
        <property type="entry name" value="GatE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017958">
        <property type="entry name" value="Gln-tRNA_amidoTrfase_suB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00134">
        <property type="entry name" value="gatE_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003107">
        <property type="entry name" value="PRK04028.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659">
        <property type="entry name" value="GLUTAMYL-TRNA GLN AMIDOTRANSFERASE SUBUNIT B MITOCHONDRIAL AND PROKARYOTIC PET112-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659:SF2">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02938">
        <property type="entry name" value="GAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02934">
        <property type="entry name" value="GatB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02637">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00845">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55261">
        <property type="entry name" value="GAD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89095">
        <property type="entry name" value="GatB/YqeY motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01234">
        <property type="entry name" value="GATB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000146944" description="Glutamyl-tRNA(Gln) amidotransferase subunit E">
        <location>
            <begin position="1"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00588"/>
        </source>
    </evidence>
    <sequence length="617" mass="66964" checksum="859331C3248953E5" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IPJ1</accession>
    <name>PSA_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Proteasome subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">20S proteasome alpha subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Proteasome core protein PsmA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">psmA</name>
        <name type="ordered locus">NP_3738A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped at one or both ends by the proteasome regulatory ATPase, PAN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323577.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IPJ1"/>
    <dbReference type="SMR" id="Q3IPJ1"/>
    <dbReference type="STRING" id="348780.NP_3738A"/>
    <dbReference type="EnsemblBacteria" id="CAI49960">
        <property type="protein sequence ID" value="CAI49960"/>
        <property type="gene ID" value="NP_3738A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703061"/>
    <dbReference type="KEGG" id="nph:NP_3738A"/>
    <dbReference type="eggNOG" id="arCOG00971">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_4_1_2"/>
    <dbReference type="OrthoDB" id="9421at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03756">
        <property type="entry name" value="proteasome_alpha_archeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000004">
        <property type="entry name" value="Proteasome subunit alpha type-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00289_A">
        <property type="entry name" value="Proteasome_A_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019982">
        <property type="entry name" value="Proteasome_asu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03633">
        <property type="entry name" value="arc_protsome_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003075">
        <property type="entry name" value="PRK03996.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0647">Proteasome</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000021793" description="Proteasome subunit alpha">
        <location>
            <begin position="1"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00289"/>
        </source>
    </evidence>
    <sequence length="255" mass="28002" checksum="E4FABF1D191E294C" modified="2005-11-08" version="1">MQGQSQQQAYDRGITIFSPDGRLYQVEYAREAVKRGTASIGIRTEDGVVLVVDKRIRSPLMERTSVEKIHKADDHIGIASAGHVADARQLIDFARRQAQVNQLRYDEPIGVETLTKAVTDHIQQYTQVGGARPFGVALIIGGIEDGEPRLYETDPSGTPYEWQALAVGADRGDIQEYLEEHYSESLDTEAGIGLALEALASVNDDSLTPEGIGLATVTVDDDDGFSQLSDEEIEAHLEERDLLEAEDDADEDDEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-09-11" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IPU8</accession>
    <name>PYRB_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aspartate carbamoyltransferase catalytic subunit</fullName>
            <ecNumber evidence="1">2.1.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Aspartate transcarbamylase</fullName>
            <shortName evidence="1">ATCase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrB</name>
        <name type="ordered locus">NP_3518A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of carbamoyl phosphate and aspartate to form carbamoyl aspartate and inorganic phosphate, the committed step in the de novo pyrimidine nucleotide biosynthesis pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20013"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterooligomer of catalytic and regulatory chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. ATCase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.2" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323470.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IPU8"/>
    <dbReference type="SMR" id="Q3IPU8"/>
    <dbReference type="STRING" id="348780.NP_3518A"/>
    <dbReference type="EnsemblBacteria" id="CAI49850">
        <property type="protein sequence ID" value="CAI49850"/>
        <property type="gene ID" value="NP_3518A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703081"/>
    <dbReference type="KEGG" id="nph:NP_3518A"/>
    <dbReference type="eggNOG" id="arCOG00911">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043846_1_2_2"/>
    <dbReference type="OrthoDB" id="7792at2157"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00116"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004070">
        <property type="term" value="F:aspartate carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000001">
        <property type="entry name" value="Aspartate carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000002">
        <property type="entry name" value="Aspartate carbamoyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00001">
        <property type="entry name" value="Asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002082">
        <property type="entry name" value="Asp_carbamoyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00670">
        <property type="entry name" value="asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002032">
        <property type="entry name" value="PRK00856.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753:SF6">
        <property type="entry name" value="ASPARTATE CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753">
        <property type="entry name" value="ORNITHINE CARBAMOYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00101">
        <property type="entry name" value="ATCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000301649" description="Aspartate carbamoyltransferase catalytic subunit">
        <location>
            <begin position="1"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00001"/>
        </source>
    </evidence>
    <sequence length="300" mass="33221" checksum="3398F320C2DCC573" modified="2005-11-08" version="1">MRHDHLLSAKQLSRGDIEGVLDRAAEIAADPDAVAEKHSDKLLGLLFFEPSTRTKMSFDTAMKRLGGDTVDMGSVDSSSVKKGESLADTVRVIEGYTDAIVLRHPSEGSPKLASEFVDVPVINAGDGAGQHPTQTLLDMYTIRENAGLDDLTIGIMGDLKYGRTVHSLAHALTNFDVSQHFISPESLQLPRSVRYDLHESGAQVREHTDIESVLETLDVLYVTRIQRERFPDENEYRQVAGEYRIDADLLESAKDDLTVMHPLPRVSEIAADVDETEHATYFKQAHNGVPVRMALLDEML</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IQI5</accession>
    <name>Q3IQI5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC-type transport system periplasmic substrate-binding protein (Probable substrate tungstate)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">tupA</name>
        <name evidence="2" type="synonym">abc22s</name>
        <name evidence="2" type="ordered locus">NP_3040A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IQI5"/>
    <dbReference type="STRING" id="348780.NP_3040A"/>
    <dbReference type="EnsemblBacteria" id="CAI49611">
        <property type="protein sequence ID" value="CAI49611"/>
        <property type="gene ID" value="NP_3040A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3040A"/>
    <dbReference type="eggNOG" id="arCOG00229">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061511_0_0_2"/>
    <dbReference type="OrthoDB" id="14917at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052738">
        <property type="entry name" value="ABC-Tungstate_binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024370">
        <property type="entry name" value="PBP_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37945">
        <property type="entry name" value="EXTRACELLULAR TUNGSTATE BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37945:SF1">
        <property type="entry name" value="EXTRACELLULAR TUNGSTATE BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12849">
        <property type="entry name" value="PBP_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PBP" evidence="1">
        <location>
            <begin position="37"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="301" mass="32003" checksum="A4DA56322F15BB50" modified="2005-11-08" version="1">MQRREFLAGGAGLTTAFTAGCADSDRANVGIADETLTLATTTSTYDTGLLDAVTLAFEDRYGVSVRTLDQGTGEALATGRRGDADVVMVHARSLEDEFIADGHGINRRDLMFNDFVVVGPDDDPAGIADLEDVTAAFATIAETASAFVSRGDNSGTHAKELAIWEAAEADPAGEWYREAGAGMGVVLNQANQTPAYALADRGTYIAQRSQLELSIQVEGPIEGGPELLANPYGVIAVNPAVHDAVNYDLAMAYIGFLTSPEGQAVIEEYTVAGEQLFFPRAVAEEPNFQQYVPEGWQPTEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-02" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IRZ4</accession>
    <name>URE3_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Urease subunit gamma</fullName>
            <ecNumber evidence="1">3.5.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Urea amidohydrolase subunit gamma</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ureA</name>
        <name type="ordered locus">NP_2012A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>urea + 2 H2O + H(+) = hydrogencarbonate + 2 NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:20557"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16199"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (urease route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the urease gamma subunit family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.5" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322726.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IRZ4"/>
    <dbReference type="SMR" id="Q3IRZ4"/>
    <dbReference type="STRING" id="348780.NP_2012A"/>
    <dbReference type="EnsemblBacteria" id="CAI49097">
        <property type="protein sequence ID" value="CAI49097"/>
        <property type="gene ID" value="NP_2012A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703450"/>
    <dbReference type="KEGG" id="nph:NP_2012A"/>
    <dbReference type="eggNOG" id="arCOG04528">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145825_1_0_2"/>
    <dbReference type="OrthoDB" id="42431at2157"/>
    <dbReference type="UniPathway" id="UPA00258">
        <property type="reaction ID" value="UER00370"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009039">
        <property type="term" value="F:urease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043419">
        <property type="term" value="P:urea catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00390">
        <property type="entry name" value="Urease_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.280.10">
        <property type="entry name" value="Urease, gamma-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00739">
        <property type="entry name" value="Urease_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012010">
        <property type="entry name" value="Urease_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002026">
        <property type="entry name" value="Urease_gamma/gamma-beta_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036463">
        <property type="entry name" value="Urease_gamma_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050069">
        <property type="entry name" value="Urease_subunit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009712">
        <property type="entry name" value="PRK13241.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00193">
        <property type="entry name" value="urease_gam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33569">
        <property type="entry name" value="UREASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33569:SF1">
        <property type="entry name" value="UREASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00547">
        <property type="entry name" value="Urease_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001223">
        <property type="entry name" value="Urease_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54111">
        <property type="entry name" value="Urease, gamma-subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000234221" description="Urease subunit gamma">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00739"/>
        </source>
    </evidence>
    <sequence length="109" mass="12549" checksum="1674FF7837637096" modified="2005-11-08" version="1">MNLTPKERERLTIFMAAELARRRKERGVKLNHPETVAYISDWACERAREGMSVAEIRQEATALLTREDVMDGVPELVDMVQVEPMFPDGTKLVTIHDPIRADTREQLEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IT31</accession>
    <name>GGGPS_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Geranylgeranylglyceryl phosphate synthase</fullName>
            <shortName evidence="1">GGGP synthase</shortName>
            <shortName evidence="1">GGGPS</shortName>
            <ecNumber evidence="1">2.5.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">(S)-3-O-geranylgeranylglyceryl phosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphoglycerol geranylgeranyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">NP_1224A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Prenyltransferase that catalyzes the transfer of the geranylgeranyl moiety of geranylgeranyl diphosphate (GGPP) to the C3 hydroxyl of sn-glycerol-1-phosphate (G1P). This reaction is the first ether-bond-formation step in the biosynthesis of archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>sn-glycerol 1-phosphate + (2E,6E,10E)-geranylgeranyl diphosphate = sn-3-O-(geranylgeranyl)glycerol 1-phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23404"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57677"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:58756"/>
            <dbReference type="EC" id="2.5.1.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GGGP/HepGP synthase family. Group I subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.41" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322339.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IT31"/>
    <dbReference type="SMR" id="Q3IT31"/>
    <dbReference type="STRING" id="348780.NP_1224A"/>
    <dbReference type="EnsemblBacteria" id="CAI48703">
        <property type="protein sequence ID" value="CAI48703"/>
        <property type="gene ID" value="NP_1224A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701088"/>
    <dbReference type="KEGG" id="nph:NP_1224A"/>
    <dbReference type="eggNOG" id="arCOG01085">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095211_0_0_2"/>
    <dbReference type="OrthoDB" id="49758at2157"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120536">
        <property type="term" value="F:heptaprenylglyceryl phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047294">
        <property type="term" value="F:phosphoglycerol geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02812">
        <property type="entry name" value="PcrB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.390">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00112">
        <property type="entry name" value="GGGP_HepGP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039074">
        <property type="entry name" value="GGGP/HepGP_synthase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038597">
        <property type="entry name" value="GGGP/HepGP_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008205">
        <property type="entry name" value="GGGP_HepGP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026417">
        <property type="entry name" value="GGGPS_halobacteria"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01768">
        <property type="entry name" value="GGGP-family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04147">
        <property type="entry name" value="GGGPS_Halobact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003199">
        <property type="entry name" value="PRK04169.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40029">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40029:SF2">
        <property type="entry name" value="HEPTAPRENYLGLYCERYL PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01884">
        <property type="entry name" value="PcrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000231309" description="Geranylgeranylglyceryl phosphate synthase">
        <location>
            <begin position="1"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>sn-glycerol 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="162"/>
            <end position="167"/>
        </location>
        <ligand>
            <name>sn-glycerol 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>sn-glycerol 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="212"/>
            <end position="213"/>
        </location>
        <ligand>
            <name>sn-glycerol 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00112"/>
        </source>
    </evidence>
    <sequence length="235" mass="25546" checksum="F25A66D97B17E4F0" modified="2005-11-08" version="1">MSAPWAEWDHIVKIDPDKTLADGETFQDVCATGTDALEIGGTTGMTEEKMARVVEATAAHDIPVYIEPSNVGAVVHDEDLDGYFVPTVLNAGDVFWTTGAHKEWVRLDGDIDWDRTFTEAYIVLNPDASVADYTEADCNLDIDEVAAYAEVAEKMFGQEIVYVEYSGMLGDPEMVQAATDATEEATVFYGGGIRDYESAYTMRQHADTVIVGDLVHDEGADAVRETVDGAKDAAD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ITD1</accession>
    <name>AATE_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">A-type ATP synthase subunit E</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpE</name>
        <name type="ordered locus">NP_1024A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Has multiple subunits with at least A(3), B(3), C, D, E, F, H, I and proteolipid K(x).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase E subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322238.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ITD1"/>
    <dbReference type="SMR" id="Q3ITD1"/>
    <dbReference type="STRING" id="348780.NP_1024A"/>
    <dbReference type="EnsemblBacteria" id="CAI48603">
        <property type="protein sequence ID" value="CAI48603"/>
        <property type="gene ID" value="NP_1024A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702542"/>
    <dbReference type="KEGG" id="nph:NP_1024A"/>
    <dbReference type="eggNOG" id="arCOG00869">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120786_0_0_2"/>
    <dbReference type="OrthoDB" id="4691at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033178">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, catalytic domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2320.30">
        <property type="entry name" value="ATP synthase, E subunit, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.620">
        <property type="entry name" value="F1F0 ATP synthase subunit B, membrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00311">
        <property type="entry name" value="ATP_synth_E_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038495">
        <property type="entry name" value="ATPase_E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002842">
        <property type="entry name" value="ATPase_V1_Esu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002629">
        <property type="entry name" value="PRK02292.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45715">
        <property type="entry name" value="ATPASE H+-TRANSPORTING V1 SUBUNIT E1A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01991">
        <property type="entry name" value="vATP-synt_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160527">
        <property type="entry name" value="V-type ATPase subunit E-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_1000059420" description="A-type ATP synthase subunit E">
        <location>
            <begin position="1"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="50"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="192" mass="21656" checksum="A5AD40EC7DAEFF2A" modified="2005-11-08" version="1">MSLDTVVEDIRDEARARADEIRSEGEERAEEIIDEAEREADDIVDEAEREAERKISQERDQKLSSAKLEAKQARLEARREVLEEVHDDVEAQIADIDGDEREALTRSLLDAAAEEFDGDSVRVHGHEDDADLLEGIVADYDGFEVGEPVDCLGGVVVESDASRVRVNNTFDSILEDVWEENLREISARLFEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-05-20" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ITR7</accession>
    <name>FAU1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable ribonuclease FAU-1</fullName>
            <ecNumber evidence="1">3.1.26.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">RNA-binding protein FAU-1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fau-1</name>
        <name type="ordered locus">NP_0750A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probable RNase involved in rRNA stability through maturation and/or degradation of precursor rRNAs. Binds to RNA in loop regions with AU-rich sequences.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAU-1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.-" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322102.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ITR7"/>
    <dbReference type="SMR" id="Q3ITR7"/>
    <dbReference type="STRING" id="348780.NP_0750A"/>
    <dbReference type="EnsemblBacteria" id="CAI48466">
        <property type="protein sequence ID" value="CAI48466"/>
        <property type="gene ID" value="NP_0750A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700951"/>
    <dbReference type="KEGG" id="nph:NP_0750A"/>
    <dbReference type="eggNOG" id="arCOG04307">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044303_0_0_2"/>
    <dbReference type="OrthoDB" id="84798at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016891">
        <property type="term" value="F:RNA endonuclease activity producing 5'-phosphomonoesters, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.380.10">
        <property type="entry name" value="FomD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01910">
        <property type="entry name" value="RNA_binding_AU_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007295">
        <property type="entry name" value="DUF402"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035930">
        <property type="entry name" value="FomD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050212">
        <property type="entry name" value="Ntdp-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016730">
        <property type="entry name" value="RNA-bd_FAU-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39159">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39159:SF1">
        <property type="entry name" value="UPF0374 PROTEIN YGAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04167">
        <property type="entry name" value="DUF402"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018644">
        <property type="entry name" value="RNA-binding_FAU-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159234">
        <property type="entry name" value="FomD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <feature type="chain" id="PRO_0000334201" description="Probable ribonuclease FAU-1">
        <location>
            <begin position="1"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="1">
        <location>
            <begin position="89"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01910"/>
        </source>
    </evidence>
    <sequence length="461" mass="49556" checksum="CD523053114DEF3B" modified="2005-11-08" version="1">MNVRVRGIYTTALTELLGGEYDIVQASPPIQRRFEASFPVAVADATVRTSDDRLGAGIAGTADAVDAVGETLSALGRDTFRWTDPAPRGAVFYGEVTETLGSGAVVDLGTVDGDSVEGFLPYNRVEGYVDEGDSYRVQVASPEPPWGDARPSLATALRIPGGLVELRRGSEGGLSETARMADLLPVDPPEGWTPNWSRAADDASLEAMEAALRRASDRAETVMEAVVDADRDEPGRIVAPQAGAWLLFGRESRFELDEYRRQIETTMPGHHRTKAATTAASAAVDFAEALCEPDGAFPFDVVTRQFGPTEGDDVTISHGKPDGRTISLGRGEVTDRDPEAESITVEREMSGGGTYDAIGVKREAGDIATTTFVEGRWWYPTVYCSADGERRGTYVNICTPLEVFPDTVRYVDLHVDVVKGPDGDVRRVDDDELDAAVEAGEISEPLAERAREVAAAIKKAL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IU78</accession>
    <name>PYRD_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dihydroorotate dehydrogenase (quinone)</fullName>
            <ecNumber evidence="1">1.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DHOdehase</fullName>
            <shortName evidence="1">DHOD</shortName>
            <shortName evidence="1">DHODase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Dihydroorotate oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrD</name>
        <name type="ordered locus">NP_0428A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-dihydroorotate + a quinone = orotate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:30187"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="1">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.2" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321941.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IU78"/>
    <dbReference type="SMR" id="Q3IU78"/>
    <dbReference type="STRING" id="348780.NP_0428A"/>
    <dbReference type="EnsemblBacteria" id="CAI48305">
        <property type="protein sequence ID" value="CAI48305"/>
        <property type="gene ID" value="NP_0428A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703083"/>
    <dbReference type="KEGG" id="nph:NP_0428A"/>
    <dbReference type="eggNOG" id="arCOG00603">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013640_2_0_2"/>
    <dbReference type="OrthoDB" id="36608at2157"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00946"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106430">
        <property type="term" value="F:dihydroorotate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04738">
        <property type="entry name" value="DHOD_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00225">
        <property type="entry name" value="DHO_dh_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050074">
        <property type="entry name" value="DHO_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012135">
        <property type="entry name" value="Dihydroorotate_DH_1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005719">
        <property type="entry name" value="Dihydroorotate_DH_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005720">
        <property type="entry name" value="Dihydroorotate_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001295">
        <property type="entry name" value="Dihydroorotate_DH_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003645">
        <property type="entry name" value="PRK05286.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003652">
        <property type="entry name" value="PRK05286.2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01036">
        <property type="entry name" value="pyrD_sub2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48109:SF4">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE (QUINONE), MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48109">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE (QUINONE), MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01180">
        <property type="entry name" value="DHO_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000164">
        <property type="entry name" value="DHO_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00911">
        <property type="entry name" value="DHODEHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00912">
        <property type="entry name" value="DHODEHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0288">FMN</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000024190" description="Dihydroorotate dehydrogenase (quinone)">
        <location>
            <begin position="1"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="249"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="67"/>
            <end position="71"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="116"/>
            <end position="120"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="242"/>
            <end position="243"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="312"/>
            <end position="313"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="350" mass="37145" checksum="A7C2BBEFBE2C5FB5" modified="2005-11-08" version="1">MGLYQLLKPALFQLDAETAHGLGHRVLDGIQGTPLERIVADYCTVVDPRLRVDAFGQTFPNPVGVAAGFDKNAEIPDALGALGFGHVEVGGVTAEPQAGNPRPRMFRLAEDRALINRMGFNNDGADIVGERLAATDCRVPVGVNIGKSKSAANDDAEADYQYTYERVADGGDYFVVNVSSPNTPGLRELQSRDRLESILGTLQDDGASPLLVKLSPDLTDAAIEDAIEVVEDLGLDGIIATNTTTKRPASLHSDAADEEGGLSGAPITDESTDIVRFIAKRTDKPIVGVGGIDDAESAYEKIRNGASVVQLYTGLVYEGPTIARDINRGLLKLLERDGFASVEDAVGVDI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IUI0</accession>
    <name>DNAK_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Chaperone protein DnaK</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">HSP70</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Heat shock 70 kDa protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Heat shock protein 70</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dnaK</name>
        <name type="ordered locus">NP_0218A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Acts as a chaperone.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321839.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IUI0"/>
    <dbReference type="SMR" id="Q3IUI0"/>
    <dbReference type="STRING" id="348780.NP_0218A"/>
    <dbReference type="EnsemblBacteria" id="CAI48200">
        <property type="protein sequence ID" value="CAI48200"/>
        <property type="gene ID" value="NP_0218A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702669"/>
    <dbReference type="KEGG" id="nph:NP_0218A"/>
    <dbReference type="eggNOG" id="arCOG03060">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005965_2_4_2"/>
    <dbReference type="OrthoDB" id="9944at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10234">
        <property type="entry name" value="ASKHA_NBD_HSP70_DnaK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000014">
        <property type="entry name" value="Chaperone protein DnaK HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000071">
        <property type="entry name" value="Molecular chaperone DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000003">
        <property type="entry name" value="Molecular chaperone DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00332">
        <property type="entry name" value="DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012725">
        <property type="entry name" value="Chaperone_DnaK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02350">
        <property type="entry name" value="prok_dnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000226036" description="Chaperone protein DnaK">
        <location>
            <begin position="1"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="488"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="579"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="492"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="523"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="584"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="636"/>
            <end position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="656" mass="69687" checksum="218117DC128BF072" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-05-14" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>X1W3M3</accession>
    <name>X1W3M3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_6146A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324452.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="X1W3M3"/>
    <dbReference type="EnsemblBacteria" id="CAI50853">
        <property type="protein sequence ID" value="CAI50853"/>
        <property type="gene ID" value="NP_6146A"/>
    </dbReference>
    <dbReference type="GeneID" id="43500108"/>
    <dbReference type="KEGG" id="nph:NP_6146A"/>
    <dbReference type="HOGENOM" id="CLU_3003248_0_0_2"/>
    <dbReference type="OrthoDB" id="256286at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="56" mass="6673" checksum="0A69269CF244487B" modified="2014-05-14" version="1">MGEGDSTNVNIELEQEDFEFLKEYKDEMGWTWEGLLKAGTPFREWKQQQYANDNPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ET83</accession>
    <name>A0A1U7ET83_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HNH nuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0002A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321731.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ET83"/>
    <dbReference type="STRING" id="348780.NP_0002A"/>
    <dbReference type="EnsemblBacteria" id="CAI48092">
        <property type="protein sequence ID" value="CAI48092"/>
        <property type="gene ID" value="NP_0002A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700735"/>
    <dbReference type="KEGG" id="nph:NP_0002A"/>
    <dbReference type="eggNOG" id="arCOG09398">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_755705_0_0_2"/>
    <dbReference type="OrthoDB" id="192298at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="228"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="366" mass="38922" checksum="75C17E0D759CB37F" modified="2017-05-10" version="1">MTDLDALAIEPLAGPPSEWSETVRSLCVHVSDDAPSASAAASWLCDRGAASRAAAERQLRFLDDLGLLYVDEECRLDERGRRLLDAAPDTEPVVLYRALSSVRGFDAAVDALGVRPLTDIEILDLLGTELDAELEDTDAVEPLLQWLVALGYLTRDGGLNELTRAGKRRSEGIDGFRERADTDGEANGTDTTSAVEASADDGLTTAPGGESHRATDTAVVADADTAADADTAAADTDTDAGAVPDAGTDSASETPPETSLLDAYDYRCMVCGDRRRRASDEGYAELHRPMPPAEGGPETAANAVVVCPNHRADLEHGLVRIDPQTLDIQHAYEDSVDGHRLTVADDHDLGAAYLAYHNDVVADIDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETC1</accession>
    <name>A0A1U7ETC1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0215 protein NP_0086A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0086A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0215 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321773.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETC1"/>
    <dbReference type="STRING" id="348780.NP_0086A"/>
    <dbReference type="EnsemblBacteria" id="CAI48134">
        <property type="protein sequence ID" value="CAI48134"/>
        <property type="gene ID" value="NP_0086A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700757"/>
    <dbReference type="KEGG" id="nph:NP_0086A"/>
    <dbReference type="eggNOG" id="arCOG00928">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095956_0_0_2"/>
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        <property type="entry name" value="Endo_dU"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="170"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="206" mass="22261" checksum="CCEC82DC489830ED" modified="2017-05-10" version="1">MKPGRRAVGIAESFRGTDDGTTESTLAGAVVRADRTVDDFVFGTCTVGGSDGTGAVADLWERLDRPDVQYVFLAGVALAWYNVVDLQVLADRIDRPVVAVSFEASDGLESAIEDAFDGDARDDRLATYRSLPPRRRLQADPPLFARSVGLGADETDRVVDAYTHERRPDPLRVAKRLAGRADDARRGGRLPAESGGVNTDGDDRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETD1</accession>
    <name>A0A1U7ETD1_NATPD</name>
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        <recommendedName>
            <fullName evidence="13">DNA-directed RNA polymerase subunit Rpo1N</fullName>
            <ecNumber evidence="13">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
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        </alternativeName>
    </protein>
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        <name evidence="13 18" type="primary">rpoA1</name>
        <name evidence="13" type="synonym">rpo1N</name>
        <name evidence="18" type="ordered locus">NP_0114A</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Forms the clamp head domain.</text>
    </comment>
    <comment type="function">
        <text evidence="14">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="13">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 13 14">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="13"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETD1"/>
    <dbReference type="STRING" id="348780.NP_0114A"/>
    <dbReference type="EnsemblBacteria" id="CAI48148">
        <property type="protein sequence ID" value="CAI48148"/>
        <property type="gene ID" value="NP_0114A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_0114A"/>
    <dbReference type="eggNOG" id="arCOG04257">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000487_3_1_2"/>
    <dbReference type="OrthoDB" id="371812at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0003677">
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="entry name" value="RNAP_archeal_A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.180">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNA polymerase II, clamp domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="RNA polymerase Rpb1, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNApol_arch_Rpo1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007080">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
    </dbReference>
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        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
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        <property type="entry name" value="RNA_pol_Rpb1_3_sf"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
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    </dbReference>
    <dbReference type="InterPro" id="IPR044893">
        <property type="entry name" value="RNA_pol_Rpb1_clamp_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038120">
        <property type="entry name" value="Rpb1_funnel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012758">
        <property type="entry name" value="RPO1N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006336">
        <property type="entry name" value="PRK08566.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02390">
        <property type="entry name" value="RNA_pol_rpoA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376:SF32">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04997">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05000">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 13 14" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="8 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 13 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 13 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 13 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase N-terminal" evidence="17">
        <location>
            <begin position="359"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1027"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="141"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1028"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="609"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="611"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="613"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00053389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAI48148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="1059" mass="118567" checksum="535E0256F00FFA46" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETF3</accession>
    <name>A0A1U7ETF3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC-type transport system ATP-binding protein (Probable substrate branched-chain amino acids)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">livG5</name>
        <name evidence="5" type="synonym">abc09a2</name>
        <name evidence="5" type="ordered locus">NP_0168A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A1U7ETF3"/>
    <dbReference type="STRING" id="348780.NP_0168A"/>
    <dbReference type="EnsemblBacteria" id="CAI48175">
        <property type="protein sequence ID" value="CAI48175"/>
        <property type="gene ID" value="NP_0168A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702393"/>
    <dbReference type="KEGG" id="nph:NP_0168A"/>
    <dbReference type="eggNOG" id="arCOG00926">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OrthoDB" id="44250at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03219">
        <property type="entry name" value="ABC_Mj1267_LivG_branched"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051120">
        <property type="entry name" value="ABC_AA/LPS_Transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45772:SF2">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45772">
        <property type="entry name" value="CONSERVED COMPONENT OF ABC TRANSPORTER FOR NATURAL AMINO ACIDS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI48175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="259" mass="28915" checksum="FE05D605C7D6310A" modified="2017-05-10" version="1">MLEARNLVKEFGKLRATDDVSLQFGTESGEMVFIVGPNGAGKTTLINLLTGLLEPDEGSVVMHEQTDDGTVERDITEMRPDRRVKEGLVRSFQIVHLFEEMTVRENVRTAALSNHDKFTKVRSLDDEHEAVEADVDALLEQFRLEDVADEVAETLPHGDRKLLDVAISFALDPNYLLLDEPTSGVGAEEKEYVIETVVEASKEQNVSTVTIEHDMDIVRTYADRMVALVEGEVFREGPPTLLDTDDDLRQTLLGVSEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETH5</accession>
    <name>A0A1U7ETH5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DNA repair helicase Rad25</fullName>
            <ecNumber evidence="7">3.6.4.12</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rad25a</name>
        <name evidence="7" type="ordered locus">NP_0212A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.4.12" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321836.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETH5"/>
    <dbReference type="STRING" id="348780.NP_0212A"/>
    <dbReference type="EnsemblBacteria" id="CAI48197">
        <property type="protein sequence ID" value="CAI48197"/>
        <property type="gene ID" value="NP_0212A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700790"/>
    <dbReference type="KEGG" id="nph:NP_0212A"/>
    <dbReference type="eggNOG" id="arCOG00874">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008213_6_0_2"/>
    <dbReference type="OrthoDB" id="11644at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17926">
        <property type="entry name" value="DEXHc_RE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050615">
        <property type="entry name" value="ATP-dep_DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040699">
        <property type="entry name" value="XPB_DRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11274:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION AND DNA REPAIR FACTOR IIH HELICASE SUBUNIT XPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11274">
        <property type="entry name" value="RAD25/XP-B DNA REPAIR HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18458">
        <property type="entry name" value="XPB_DRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="85"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="321"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="450" mass="50098" checksum="745B53CA0E2834AE" modified="2017-05-10" version="1">MVTLRYDEGTVRIDGDGTERLASLSFVEADPRSESHRAPAFRYPFLRRTCRDRGLAVEDRVLDTPAVDGLSTTYELRGYQREALDAWRSHGDRGVVVLPTGSGKTVLAVGAIAALETPTLVVVPTVDLLDQWHEELSAEFGDATAVGRLGGGVQRIEPLTVATYDSAYLRAEDLGNRFGLVVFDEVHHLGGEGYRDAARLSAAPARLGLTATFERPDGEHEVIETLVGPVVYERSAEDLAGDHLADYDIRRLEVPLTPEERDIYERHQQTFTEYLASSDIQLRSGSDYQELVKRSGSDPKAREALLAKQRAREVMMNAERKLTVLADILDRHDGDRVIVFTAYTDLVYDIAERFLIPPITSEMGTDERREILDRFRRGDYSRVVAANVLDEGVDVPDANVAVVLSGSGSQREFTQRLGRVLRPSDDGGRALLYEVVAEETAEERVARRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETH8</accession>
    <name>A0A1U7ETH8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0230A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321845.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETH8"/>
    <dbReference type="STRING" id="348780.NP_0230A"/>
    <dbReference type="EnsemblBacteria" id="CAI48206">
        <property type="protein sequence ID" value="CAI48206"/>
        <property type="gene ID" value="NP_0230A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700795"/>
    <dbReference type="KEGG" id="nph:NP_0230A"/>
    <dbReference type="HOGENOM" id="CLU_2340260_0_0_2"/>
    <dbReference type="OrthoDB" id="242071at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="97" mass="10139" checksum="FAE3DB22C181BFE0" modified="2017-05-10" version="1">MANDDDRQPLFGPELFVPTEFLADNMDHNVDVTGATSLEVEHSLDIDVPVRRIEVGVIAFAVLVVLLGVFAPVGATVQVSAALGAVAAGNLLVRTLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETM0</accession>
    <name>A0A1U7ETM0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0344A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48263.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939404.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETM0"/>
    <dbReference type="EnsemblBacteria" id="CAI48263">
        <property type="protein sequence ID" value="CAI48263"/>
        <property type="gene ID" value="NP_0344A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700825"/>
    <dbReference type="KEGG" id="nph:NP_0344A"/>
    <dbReference type="eggNOG" id="arCOG09162">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2985767_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48263.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="59" mass="6407" checksum="B500336456744579" modified="2017-05-10" version="1">MPDTVTPVTPKKTSLGNKILDSVIGIGAMLLLVPLAPFLILIWLWDRFRSDGDDGPADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETP0</accession>
    <name>A0A1U7ETP0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NUDIX family hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_0392A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321925.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETP0"/>
    <dbReference type="STRING" id="348780.NP_0392A"/>
    <dbReference type="EnsemblBacteria" id="CAI48287">
        <property type="protein sequence ID" value="CAI48287"/>
        <property type="gene ID" value="NP_0392A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702987"/>
    <dbReference type="KEGG" id="nph:NP_0392A"/>
    <dbReference type="eggNOG" id="arCOG01073">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062658_8_1_2"/>
    <dbReference type="OrthoDB" id="192849at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006753">
        <property type="term" value="P:nucleoside phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019693">
        <property type="term" value="P:ribose phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03424">
        <property type="entry name" value="NUDIX_ADPRase_Nudt5_UGPPase_Nudt14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11839:SF18">
        <property type="entry name" value="NUDIX HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11839">
        <property type="entry name" value="UDP/ADP-SUGAR PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="3">
        <location>
            <begin position="48"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="194" mass="21388" checksum="04668C4CC6FE0998" modified="2017-05-10" version="1">MTDDYSPADHDWTVIESETEYETGWYTGGYDLVELPDGRTKRYYWAELPPATVIVAVLDDPAALGFDSEERSVVMVEQFRPTIRKLCRELPAGIVESGEDFAEAGRRELEEEAGLVADSVDLLSSFWCSTGVLRHSRGIVYADDLSVGERRLDGNEFLDVMTVPVEAALDVARGPPANDATIEGLLLADEADLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETQ8</accession>
    <name>A0A1U7ETQ8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Homolog to DNA topoisomerase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0446A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321950.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETQ8"/>
    <dbReference type="STRING" id="348780.NP_0446A"/>
    <dbReference type="EnsemblBacteria" id="CAI48314">
        <property type="protein sequence ID" value="CAI48314"/>
        <property type="gene ID" value="NP_0446A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700858"/>
    <dbReference type="KEGG" id="nph:NP_0446A"/>
    <dbReference type="eggNOG" id="arCOG01305">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1131576_0_0_2"/>
    <dbReference type="OrthoDB" id="190320at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.180.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048302">
        <property type="entry name" value="NucS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049173">
        <property type="entry name" value="NucS_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21003">
        <property type="entry name" value="NucS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease NucS N-terminal PH-like" evidence="1">
        <location>
            <begin position="3"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="240" mass="25386" checksum="B724E5B1EC5818FD" modified="2017-05-10" version="1">MPIRTITGDCLVRFEGPRDRTVRGTVLAVVKPDNTVLVHDIDGYQPAAWLTRASELSLTRDPLWLVAVDGDETLRIEAVGDVTVTEHEATVAGTPVGNCRCGGDLVRSGGGVSCLDCGERFGLPSGATTTGDDCSCGLPTVRVDRGEPFELCLDPDCGSLLEAVRAAFEGEWACPECGSDLDIIRRGGLLAGCEQYPDCETAFSIPDGVVDGRCGCGLPVFETATGRRCLDTDCSVGRDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETR3</accession>
    <name>A0A1U7ETR3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Probable coiled-coil protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_0440A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321947.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETR3"/>
    <dbReference type="STRING" id="348780.NP_0440A"/>
    <dbReference type="EnsemblBacteria" id="CAI48311">
        <property type="protein sequence ID" value="CAI48311"/>
        <property type="gene ID" value="NP_0440A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700857"/>
    <dbReference type="KEGG" id="nph:NP_0440A"/>
    <dbReference type="eggNOG" id="arCOG04573">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_363958_0_0_2"/>
    <dbReference type="OrthoDB" id="242713at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1480.30">
        <property type="entry name" value="Designed four-helix bundle protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47357">
        <property type="entry name" value="COP1-INTERACTIVE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47357:SF1">
        <property type="entry name" value="SPINDLE POLE BODY COMPONENT 110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58104">
        <property type="entry name" value="Methyl-accepting chemotaxis protein (MCP) signaling domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="Methyl-accepting transducer" evidence="4">
        <location>
            <begin position="519"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="92"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="128"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="653"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="513"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="727"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="141"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="178"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="218"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="245"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="265"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="286"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="298"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="316"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="347"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="386"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI48311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="767" mass="79346" checksum="E718E18138405FFC" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETZ3</accession>
    <name>A0A1U7ETZ3_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF8142 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0638A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322046.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETZ3"/>
    <dbReference type="STRING" id="348780.NP_0638A"/>
    <dbReference type="EnsemblBacteria" id="CAI48410">
        <property type="protein sequence ID" value="CAI48410"/>
        <property type="gene ID" value="NP_0638A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700915"/>
    <dbReference type="KEGG" id="nph:NP_0638A"/>
    <dbReference type="eggNOG" id="arCOG07498">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_174506_2_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058455">
        <property type="entry name" value="DUF8142"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26465">
        <property type="entry name" value="DUF8142"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="DUF8142" evidence="2">
        <location>
            <begin position="8"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="72" mass="7590" checksum="FC89DB86D074E546" modified="2017-05-10" version="1">MTEAADAADESPSRAKTATVVILPFLLLGLGNVVLILQWGLNLVWGLLLVPPVLFVSALGWLAVRGGIGEEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU03</accession>
    <name>A0A1U7EU03_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Halorhodopsin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">hop</name>
        <name evidence="12" type="ordered locus">NP_0642A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal/bacterial/fungal opsin family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322048.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU03"/>
    <dbReference type="STRING" id="348780.NP_0642A"/>
    <dbReference type="EnsemblBacteria" id="CAI48412">
        <property type="protein sequence ID" value="CAI48412"/>
        <property type="gene ID" value="NP_0642A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702828"/>
    <dbReference type="KEGG" id="nph:NP_0642A"/>
    <dbReference type="eggNOG" id="arCOG02811">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054785_5_1_2"/>
    <dbReference type="OrthoDB" id="186372at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009881">
        <property type="term" value="F:photoreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007602">
        <property type="term" value="P:phototransduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15243">
        <property type="entry name" value="7tm_Halorhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001425">
        <property type="entry name" value="Arc/bac/fun_rhodopsins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018229">
        <property type="entry name" value="Rhodopsin_retinal_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28286">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28286:SF2">
        <property type="entry name" value="BACTERIORHODOPSIN _OPSIN, NOPA (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01036">
        <property type="entry name" value="Bac_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00251">
        <property type="entry name" value="BACTRLOPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01021">
        <property type="entry name" value="Bac_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00950">
        <property type="entry name" value="BACTERIAL_OPSIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0157">Chromophore</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0681">Retinal protein</keyword>
    <keyword evidence="4" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="216"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="245"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI48412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="291" mass="30963" checksum="6E801B4D74782D5E" modified="2017-05-10" version="1">MTETLPPVTESAVALQAEVTQRELFEFVLNDPLLASSLYINIALAGLSILLFVFMTRGLDDPRAKLIAVSTILVPVVSIASYTGLASGLTISVLEMPAGHFAEGSSVMLGGEEVDGVVTMWGRYLTWALSTPMILLALGLLAGSNATKLFTAITFDIAMCVTGLAAALTTSSHLMRWFWYAISCACFIVVLYILLVEWAQDAKAAGTADIFSTLKLLTVVMWLGYPIVWALGVEGVAVLPVGYTSWAYSALDIVAKYIFAFLLLNYLTSNEGVVSGSILDVPSASGAPADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU56</accession>
    <name>A0A1U7EU56_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alpha/beta hydrolase fold protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0792A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322123.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU56"/>
    <dbReference type="STRING" id="348780.NP_0792A"/>
    <dbReference type="ESTHER" id="natpd-q3itp6">
        <property type="family name" value="6_AlphaBeta_hydrolase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI48487">
        <property type="protein sequence ID" value="CAI48487"/>
        <property type="gene ID" value="NP_0792A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700965"/>
    <dbReference type="KEGG" id="nph:NP_0792A"/>
    <dbReference type="eggNOG" id="arCOG01648">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_50_1_2"/>
    <dbReference type="OrthoDB" id="111592at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050266">
        <property type="entry name" value="AB_hydrolase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798:SF31">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN YCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798">
        <property type="entry name" value="MONOACYLGLYCEROL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="25"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="259" mass="27931" checksum="EC1B8692CBCE1966" modified="2017-05-10" version="1">MPTVTTDDTTLYYERSDHDGPTVAFIPDVGFGPWVWGWQAPSLSGRYETLIYAQRGTDGSDVAGPYTIDRLAADLEAVLADAGVRRVHLVGAGLGAMVALRYGHAYSRARSLSLFGAAADGDRIDAEALEALHPADQTRYRESLSLAFTDRFLVETGATDDIVEWRRAEDATGEALAGHREAALSFEARPLYEFSLPTLVCHGVDDPVVPQAAGEALAKDLPHGRFEPVEGKRCCYIEQSVAVTDALDGFIDAVDADAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUC4</accession>
    <name>A0A1U7EUC4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Small CPxCG-related zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0928A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322191.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUC4"/>
    <dbReference type="STRING" id="348780.NP_0928A"/>
    <dbReference type="EnsemblBacteria" id="CAI48555">
        <property type="protein sequence ID" value="CAI48555"/>
        <property type="gene ID" value="NP_0928A"/>
    </dbReference>
    <dbReference type="GeneID" id="54763309"/>
    <dbReference type="KEGG" id="nph:NP_0928A"/>
    <dbReference type="eggNOG" id="arCOG06423">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2968402_0_0_2"/>
    <dbReference type="OrthoDB" id="154591at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049695">
        <property type="entry name" value="HVO_0416-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041910">
        <property type="entry name" value="HVO_0416"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="55" mass="6135" checksum="3C62290AE3141DF6" modified="2017-05-10" version="1">MAATNDIFDDFLDDRGHETESPTWEEDYNKKRCPDCGGLHGLDASVCSVCGWQPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUG0</accession>
    <name>A0A1U7EUG0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">CobW domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">NP_1036A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SIMIBI class G3E GTPase family. ZNG1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322244.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUG0"/>
    <dbReference type="STRING" id="348780.NP_1036A"/>
    <dbReference type="EnsemblBacteria" id="CAI48609">
        <property type="protein sequence ID" value="CAI48609"/>
        <property type="gene ID" value="NP_1036A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701033"/>
    <dbReference type="KEGG" id="nph:NP_1036A"/>
    <dbReference type="eggNOG" id="arCOG01233">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017452_2_0_2"/>
    <dbReference type="OrthoDB" id="359387at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03112">
        <property type="entry name" value="CobW-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1220.10">
        <property type="entry name" value="CobW-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036627">
        <property type="entry name" value="CobW-likC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011629">
        <property type="entry name" value="CobW-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051927">
        <property type="entry name" value="Zn_Chap_cDPG_Synth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43603">
        <property type="entry name" value="COBW DOMAIN-CONTAINING PROTEIN DDB_G0274527"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43603:SF1">
        <property type="entry name" value="ZINC-REGULATED GTPASE METALLOPROTEIN ACTIVATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07683">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00833">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobW C-terminal" evidence="6">
        <location>
            <begin position="256"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="401" mass="44596" checksum="68CAEB9259C1A0D6" modified="2017-05-10" version="1">MSDTIPVTVLSGALGAGKTTTLNHVLTADHGLEVAVLVNDMGEVNVDVDHVDQQSQLSQTDEEVIEMSNGCICCRLRGDMLDAVGSLAASREFDYLLVESSGISEPIPVAQTFTLGFEDADYDPTTDYHLDTMVSVVDAHAMYEGFDSGAALADETAGGGTDRVPEEVLMDQIEFCDVLLLNKCDLVPDDELDAIEAVLETLQPRAEILRTEFGAVDPTDILDTGRFDFEMARDSAGWKHELQHDHHHDPSEEHGVESFVFRANRPVHPERFESLLYDLPESVIRAKGFFWSAGREDISMGLDKAGQSVRAGPNGRWIATLPPQEQRRYLQALPGLEDEWDDQWGDRGAELVFIGRNYDPQQLEARLEACLLTDDEMDDDWSRYPDPFGTERQREFALADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUH6</accession>
    <name>A0A1U7EUH6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_1046A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322249.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUH6"/>
    <dbReference type="STRING" id="348780.NP_1046A"/>
    <dbReference type="EnsemblBacteria" id="CAI48614">
        <property type="protein sequence ID" value="CAI48614"/>
        <property type="gene ID" value="NP_1046A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701037"/>
    <dbReference type="KEGG" id="nph:NP_1046A"/>
    <dbReference type="eggNOG" id="arCOG03919">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059308_0_0_2"/>
    <dbReference type="OrthoDB" id="71082at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="41"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="254" mass="26819" checksum="7CBB32B92E805A27" modified="2017-05-10" version="1">MDAPLVAGVIALGMVHGVLPDHGWPIAAVYALDQPRQWVSGTVAALVLGVGHLISSVALVAAYVSFSAFASFAEGPWMKPLAGGLLVLLGIYEYVSGGHVHEHGDGEDAHHDHDHGHGGDHGSHEHSDVHHEHGADNIVGTVRSYLPDGGGHAHLDESDAERGLRTLGVTALVLGFAHEEPIQVLAICAGTEFCLELMLIYSVAVIVAILVPTLLLIAGYQTHRERIEQYTPYLPTLTAAVLVIVGLGFITGVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUN1</accession>
    <name>A0A1U7EUN1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Probable glycosyltransferase, type 2</fullName>
            <ecNumber evidence="5">2.4.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">gtl4</name>
        <name evidence="5" type="ordered locus">NP_1180A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.-.-" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322317.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUN1"/>
    <dbReference type="STRING" id="348780.NP_1180A"/>
    <dbReference type="EnsemblBacteria" id="CAI48681">
        <property type="protein sequence ID" value="CAI48681"/>
        <property type="gene ID" value="NP_1180A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701077"/>
    <dbReference type="KEGG" id="nph:NP_1180A"/>
    <dbReference type="eggNOG" id="arCOG01388">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077872_0_0_2"/>
    <dbReference type="OrthoDB" id="196370at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43179:SF12">
        <property type="entry name" value="GALACTOFURANOSYLTRANSFERASE GLFT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43179">
        <property type="entry name" value="RHAMNOSYLTRANSFERASE WBBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="4">
        <location>
            <begin position="4"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI48681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="299" mass="32118" checksum="A861AE68ABA051C9" modified="2017-05-10" version="1">MEVSVVVPTLNDREELRGCLDALAAESPAEVVVVNGPSTDGTSGMVRDRDDVAVLVEVDDRNVNAARNAGLDRARGDVVAFVNPSLTVESGWHGAVVDALSDADAVTGPTHEQLQAGVATDTAESRRIRGRTVTYFNGGNVAFTDETLEDIDGFDERLDVGGARDAAHRIAGVDREVAWSAEMCVAREAAADGGRTERGWRSRYRSLTYRLAKNYGPHPTVVIRTLRHALDDAASAFRDVARGDAKPTVWLGNGRDVAVGGVSGFTDGLRARYADRSPARNPAGWSDRTDRAVAVYDRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUN5</accession>
    <name>A0A1U7EUN5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">5'-deoxynucleotidase</fullName>
            <ecNumber evidence="5">3.1.3.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">NP_1206A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyribonucleoside 5'-phosphate + H2O = a 2'-deoxyribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:36167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18274"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:65317"/>
            <dbReference type="EC" id="3.1.3.89"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.89" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322330.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUN5"/>
    <dbReference type="STRING" id="348780.NP_1206A"/>
    <dbReference type="EnsemblBacteria" id="CAI48694">
        <property type="protein sequence ID" value="CAI48694"/>
        <property type="gene ID" value="NP_1206A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701082"/>
    <dbReference type="KEGG" id="nph:NP_1206A"/>
    <dbReference type="eggNOG" id="arCOG04311">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039453_1_1_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002953">
        <property type="term" value="F:5'-deoxynucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039356">
        <property type="entry name" value="YfbR/HDDC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11845">
        <property type="entry name" value="5'-DEOXYNUCLEOTIDASE HDDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11845:SF13">
        <property type="entry name" value="5'-DEOXYNUCLEOTIDASE HDDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13023">
        <property type="entry name" value="HD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD/PDEase" evidence="8">
        <location>
            <begin position="33"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI48694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="198" mass="22248" checksum="8E7C0215BFF284D0" modified="2017-05-10" version="1">MTGDIDAALSALADAYALKDERRTGWQLRAVENPESVAAHSWGVATLVVRFCPDDLDRERALSLAVVHDIAEAEVGDIPTRADPDADTVDDEEKVRRERAALSGPLAGLGDDIRELWEAYERRDSPEARFVKDMDLLDTCLQALVYERDGRYDADEANPHFEAYDGLDEFFATSEPRLSTDRGRDLFERVRARYEALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUU8</accession>
    <name>A0A1U7EUU8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">CDP-alcohol 1-archaetidyltransferase</fullName>
            <ecNumber evidence="4">2.7.8.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pgsA1</name>
        <name evidence="4" type="ordered locus">NP_1334A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.-" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322393.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUU8"/>
    <dbReference type="STRING" id="348780.NP_1334A"/>
    <dbReference type="EnsemblBacteria" id="CAI48758">
        <property type="protein sequence ID" value="CAI48758"/>
        <property type="gene ID" value="NP_1334A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701116"/>
    <dbReference type="KEGG" id="nph:NP_1334A"/>
    <dbReference type="eggNOG" id="arCOG00672">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082036_0_0_2"/>
    <dbReference type="OrthoDB" id="331608at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016780">
        <property type="term" value="F:phosphotransferase activity, for other substituted phosphate groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2 4" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="69"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="132"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="217"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="250" mass="26121" checksum="DA6AFA49FAE7172F" modified="2017-05-10" version="1">MDALPVSDAPVIRPRFVALGAFGAGVPVLAALRVSVLWAAIAVAVWLCILGYAWHLLGVTRRSERAPWVRLGVANAVTLFRGWLLALLAGAAVVSAPPMGLVTSLFVLAVSLDAVDGAIARRTHETTLGARLDAAMDALAVLVGSVVAVSVGALPWWYLVAGGVWYAYAGSLSLRKRAGRPVFSLPESRLRPAIGVSQFLIIGVALAPVAVLSVPDVVVAIALGALCVSFARDWAAATGRLGECQEQRDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUW0</accession>
    <name>A0A1U7EUW0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Coenzyme A biosynthesis bifunctional protein CoaBC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">DNA/pantothenate metabolism flavoprotein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Phosphopantothenoylcysteine synthetase/decarboxylase</fullName>
            <shortName evidence="3">PPCS-PPCDC</shortName>
        </alternativeName>
        <domain>
            <recommendedName>
                <fullName evidence="3">Phosphopantothenoylcysteine decarboxylase</fullName>
                <shortName evidence="3">PPC decarboxylase</shortName>
                <shortName evidence="3">PPC-DC</shortName>
                <ecNumber evidence="3">4.1.1.36</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="3">CoaC</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="3">Phosphopantothenate--cysteine ligase</fullName>
                <ecNumber evidence="3">6.3.2.5</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="3">CoaB</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="3">Phosphopantothenoylcysteine synthetase</fullName>
                <shortName evidence="3">PPC synthetase</shortName>
                <shortName evidence="3">PPC-S</shortName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="3 6" type="primary">coaBC</name>
        <name evidence="6" type="ordered locus">NP_1374A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes two sequential steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine. In the second step the latter compound is decarboxylated to form 4'-phosphopantotheine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(R)-4'-phosphopantothenate + L-cysteine + CTP = N-[(R)-4-phosphopantothenoyl]-L-cysteine + CMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19397"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:59458"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="6.3.2.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>N-[(R)-4-phosphopantothenoyl]-L-cysteine + H(+) = (R)-4'-phosphopantetheine + CO2</text>
            <dbReference type="Rhea" id="RHEA:16793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:59458"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="EC" id="4.1.1.36"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="3">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the PPC synthetase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.36" evidence="3"/>
    <dbReference type="EC" id="6.3.2.5" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322413.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUW0"/>
    <dbReference type="STRING" id="348780.NP_1374A"/>
    <dbReference type="EnsemblBacteria" id="CAI48778">
        <property type="protein sequence ID" value="CAI48778"/>
        <property type="gene ID" value="NP_1374A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702664"/>
    <dbReference type="KEGG" id="nph:NP_1374A"/>
    <dbReference type="eggNOG" id="arCOG01704">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033319_0_3_2"/>
    <dbReference type="OrthoDB" id="10536at2157"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071513">
        <property type="term" value="C:phosphopantothenoylcysteine decarboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:FMN binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004632">
        <property type="term" value="F:phosphopantothenate--cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004633">
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015941">
        <property type="term" value="P:pantothenate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10300">
        <property type="entry name" value="CoaB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="entry name" value="Flavin prenyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02225">
        <property type="entry name" value="CoaBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035929">
        <property type="entry name" value="CoaB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005252">
        <property type="entry name" value="CoaBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007085">
        <property type="entry name" value="DNA/pantothenate-metab_flavo_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00521">
        <property type="entry name" value="coaBC_dfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359">
        <property type="entry name" value="HOMO-OLIGOMERIC FLAVIN CONTAINING CYS DECARBOXYLASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359:SF6">
        <property type="entry name" value="PHOSPHOPANTOTHENOYLCYSTEINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04127">
        <property type="entry name" value="DFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102645">
        <property type="entry name" value="CoaB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="Homo-oligomeric flavin-containing Cys decarboxylases, HFCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="3 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 3 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavoprotein" evidence="4">
        <location>
            <begin position="6"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="DNA/pantothenate metabolism flavoprotein C-terminal" evidence="5">
        <location>
            <begin position="179"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphopantothenoylcysteine decarboxylase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphopantothenate--cysteine ligase" evidence="3">
        <location>
            <begin position="184"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI48778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="385" mass="40009" checksum="451A51CD75514DA0" modified="2017-05-10" version="1">MLAGTNVALGVSGSIAAVKTVELAHELRRQGAAVRAVMTDAARGIVHPWAVEFATDNPVVTEITGSVEHVELCGYDGWADVLLLAPATANTVGKVAAAVDDTPVTTTATTALGSDVPIVVAPAMHEPMYDHPGVLEAIETVEAWGVDFIDPRIEEGKAKIASEEAIVLGVARAASDQPLAGRHVVVTSGATSESIDPIRVLTNRSSGRTGREVAKACYVRGADVTLVHDGETVPYADVHRVESAAEMLDAVQAVADGADALVSAAAIGDYTVETADEKIRSGQTLTLELEPTPKLIDSVREAHPGLAIVGFKAETSGDDDAMVGKARETLDRADLDFVVANDASVMGESRTRVLFVDETDVTAHEGDKAAIGGHIADELASVLEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUY0</accession>
    <name>A0A1U7EUY0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Peroxiredoxin domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_1418A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322435.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUY0"/>
    <dbReference type="STRING" id="348780.NP_1418A"/>
    <dbReference type="EnsemblBacteria" id="CAI48800">
        <property type="protein sequence ID" value="CAI48800"/>
        <property type="gene ID" value="NP_1418A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701143"/>
    <dbReference type="KEGG" id="nph:NP_1418A"/>
    <dbReference type="eggNOG" id="arCOG00315">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_14_2_2"/>
    <dbReference type="OrthoDB" id="334647at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016209">
        <property type="term" value="F:antioxidant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050455">
        <property type="entry name" value="Tpx_Peroxidase_subfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43110">
        <property type="entry name" value="THIOL PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43110:SF1">
        <property type="entry name" value="THIOL PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="16"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="171" mass="19128" checksum="AB678CAF5440A242" modified="2017-05-10" version="1">MNLDFDVVELPVADPPSPGEDAPDFTRPLVTDEYWEDTSLAELTDDGPVVLVCHTMDGDFPATYIWQEIRDRNWDDYDAAVVGLSISSPYEHARFIDEWNLHAFRLFSDPANGVGEAYGISHDLDGMAGIEEPRPAVFVIDSEGTVQYTWAAAEWPEFPPYDDIEAAIESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV43</accession>
    <name>A0A1U7EV43_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Small CPxCG-related zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1566A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322508.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV43"/>
    <dbReference type="STRING" id="348780.NP_1566A"/>
    <dbReference type="EnsemblBacteria" id="CAI48874">
        <property type="protein sequence ID" value="CAI48874"/>
        <property type="gene ID" value="NP_1566A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701186"/>
    <dbReference type="KEGG" id="nph:NP_1566A"/>
    <dbReference type="eggNOG" id="arCOG06412">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2802377_0_0_2"/>
    <dbReference type="OrthoDB" id="284396at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055982">
        <property type="entry name" value="DUF7560"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24441">
        <property type="entry name" value="DUF7560"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="67" mass="6957" checksum="D552B2791BCEB9C4" modified="2017-05-10" version="1">MSQGDADATFVCPECRSEVPVTPPMRTALLDEGCVVCGAAVSADAFGTVATGRRESAPEARHPTHPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV62</accession>
    <name>A0A1U7EV62_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Threonine synthase</fullName>
            <ecNumber evidence="3">4.2.3.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">thrC3</name>
        <name evidence="3" type="ordered locus">NP_1632A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.2.3.1" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322541.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV62"/>
    <dbReference type="STRING" id="348780.NP_1632A"/>
    <dbReference type="EnsemblBacteria" id="CAI48907">
        <property type="protein sequence ID" value="CAI48907"/>
        <property type="gene ID" value="NP_1632A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703255"/>
    <dbReference type="KEGG" id="nph:NP_1632A"/>
    <dbReference type="eggNOG" id="arCOG01434">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028142_4_1_2"/>
    <dbReference type="OrthoDB" id="341080at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004795">
        <property type="term" value="F:threonine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="2">
        <location>
            <begin position="64"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="164"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="388" mass="40543" checksum="7126E2E4F6BF1533" modified="2017-05-10" version="1">MATFECTDCGTVDDDVPTRCPDCAGVLNAVYDYDTIDLTQAALADRDGTWKHHELLPFVPDGIGEGTTPLVAAPRLADELGVGQVFIKNEAHNPTGTITDRAAALVAAAAERADADTLALPTTGNGGQSAAAYAARAGLLSRSFVPSRANFINKAMINVHGGDMRVVEGRYDDAREAYQTAREELPAADEEAWFPAGPFDSPFRHEGLKPLYYELVEQLDWTVPDAVVAPTGHGEAVAGLWKGAREFESVGLTESTPRLYAAQPDGCAPIVDALDTDSDPEPWSVPDTVVGSLEIPDPAGGLPAVEAVRASGGEGVAVDDQDLLDSAATVAQHEGLEVSIACGAAAAGAWDLAEEGAFDADDTVVLVNTGTGNKDADIVRSRLMSQGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV63</accession>
    <name>A0A1U7EV63_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1612A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322531.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV63"/>
    <dbReference type="STRING" id="348780.NP_1612A"/>
    <dbReference type="EnsemblBacteria" id="CAI48897">
        <property type="protein sequence ID" value="CAI48897"/>
        <property type="gene ID" value="NP_1612A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701201"/>
    <dbReference type="KEGG" id="nph:NP_1612A"/>
    <dbReference type="eggNOG" id="arCOG04784">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2433758_0_0_2"/>
    <dbReference type="OrthoDB" id="333484at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054623">
        <property type="entry name" value="HAH_0734-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045545">
        <property type="entry name" value="HAH_0734_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23384">
        <property type="entry name" value="DUF7098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="89" mass="9999" checksum="97A6F76E7B8EF241" modified="2017-05-10" version="1">MKQLIIHGDPGIRKGAVIEHDGTEYVCFSISRQGEWHGPEEVQLWCTVGTEDEREAFERREFIPMRLETVSVDADDVTVVKAKADPATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVB3</accession>
    <name>A0A1U7EVB3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">GNAT family acetyltransferase</fullName>
            <ecNumber evidence="5">2.3.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_1712A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.-" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322580.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVB3"/>
    <dbReference type="STRING" id="348780.NP_1712A"/>
    <dbReference type="EnsemblBacteria" id="CAI48947">
        <property type="protein sequence ID" value="CAI48947"/>
        <property type="gene ID" value="NP_1712A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701233"/>
    <dbReference type="KEGG" id="nph:NP_1712A"/>
    <dbReference type="eggNOG" id="arCOG00834">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102818_0_0_2"/>
    <dbReference type="OrthoDB" id="51421at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050832">
        <property type="entry name" value="Bact_Acetyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43877:SF2">
        <property type="entry name" value="AMINOALKYLPHOSPHONATE N-ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43877">
        <property type="entry name" value="AMINOALKYLPHOSPHONATE N-ACETYLTRANSFERASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="4">
        <location>
            <begin position="28"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI48947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="178" mass="20329" checksum="1DD62445EAAADE5B" modified="2017-05-10" version="1">MTREYPTEIAGPYEAPPRETEDRDERRIEVRRYDDEFERLIDMYRAFDPEDRAQGIPPANESAIRNWLEQLLGDDDNPGINVIAWHDDEPIGHAMLIPDRHGAYELAIFVLHEYQGSGIGTELIQALLGAGREEGVERVWLTVERWNTPATALYRKVGFESTGDGGFEIEMAARLVDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVB9</accession>
    <name>A0A1U7EVB9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Homolog to translation elongation factor aEF-1 alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">NP_1740A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322594.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVB9"/>
    <dbReference type="STRING" id="348780.NP_1740A"/>
    <dbReference type="EnsemblBacteria" id="CAI48961">
        <property type="protein sequence ID" value="CAI48961"/>
        <property type="gene ID" value="NP_1740A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701245"/>
    <dbReference type="KEGG" id="nph:NP_1740A"/>
    <dbReference type="eggNOG" id="arCOG01562">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012821_1_2_2"/>
    <dbReference type="OrthoDB" id="30874at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03694">
        <property type="entry name" value="GTPBP_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03708">
        <property type="entry name" value="GTPBP_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050055">
        <property type="entry name" value="EF-Tu_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004160">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721">
        <property type="entry name" value="ELONGATION FACTOR TU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721:SF9">
        <property type="entry name" value="GTP-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03143">
        <property type="entry name" value="GTP_EFTU_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 7" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="6">
        <location>
            <begin position="124"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="534" mass="57230" checksum="9123722EA07682C4" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVG2</accession>
    <name>A0A1U7EVG2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF8052 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1830A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49006.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939483.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVG2"/>
    <dbReference type="STRING" id="348780.NP_1830A"/>
    <dbReference type="EnsemblBacteria" id="CAI49006">
        <property type="protein sequence ID" value="CAI49006"/>
        <property type="gene ID" value="NP_1830A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701268"/>
    <dbReference type="KEGG" id="nph:NP_1830A"/>
    <dbReference type="eggNOG" id="arCOG04724">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1253564_0_0_2"/>
    <dbReference type="OrthoDB" id="210068at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058365">
        <property type="entry name" value="DUF8052"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26226">
        <property type="entry name" value="DUF8052"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8052" evidence="1">
        <location>
            <begin position="23"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49006.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="202" mass="22731" checksum="BCB6CB541EBF465F" modified="2017-05-10" version="1">MSQTETEAGAEAPAGDDIPVWDDEYIEAVSKRLCHHYDLEKNRRVEGTTFSLYGEMVLENTKHFLHPAITIARHESREHLFVQRVAELTEADLDRLVATGHALADEWIDADGEHYSTEFTFVTVVPSLSEDVRSAVQSLDERTLLKYGYHGHYEVNLIVVAPDAGELVANEGVDVAEAFATWDEIERTEPGLLQLVARRLQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVG5</accession>
    <name>A0A1U7EVG5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF8107 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_1824A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322635.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVG5"/>
    <dbReference type="EnsemblBacteria" id="CAI49003">
        <property type="protein sequence ID" value="CAI49003"/>
        <property type="gene ID" value="NP_1824A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701265"/>
    <dbReference type="KEGG" id="nph:NP_1824A"/>
    <dbReference type="eggNOG" id="arCOG07499">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_200164_0_0_2"/>
    <dbReference type="OrthoDB" id="214676at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058420">
        <property type="entry name" value="DUF8107"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26409">
        <property type="entry name" value="DUF8107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="DUF8107" evidence="3">
        <location>
            <begin position="8"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF26409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="77" mass="8487" checksum="7A13E31E9545ACAC" modified="2017-05-10" version="1">MSQGESNDAEKDSGSLKEGLSESAGDPRVHLVMNLLLSAMFVYTLLWGLDFLGTLEFTRLRFAFGTVVMMGLTRTLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVM3</accession>
    <name>A0A1U7EVM3_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CTP synthetase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1972A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322707.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVM3"/>
    <dbReference type="STRING" id="348780.NP_1972A"/>
    <dbReference type="EnsemblBacteria" id="CAI49077">
        <property type="protein sequence ID" value="CAI49077"/>
        <property type="gene ID" value="NP_1972A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701324"/>
    <dbReference type="KEGG" id="nph:NP_1972A"/>
    <dbReference type="eggNOG" id="arCOG04783">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170794_0_0_2"/>
    <dbReference type="OrthoDB" id="302988at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055550">
        <property type="entry name" value="DUF7126"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23443">
        <property type="entry name" value="DUF7126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="106" mass="10910" checksum="B91EF42F7432CBC6" modified="2017-05-10" version="1">MSEVVIAGPDPEGLGEALEAEGASVSHADGTATRPDLEDAGIVDADVFVVTDAGLATSVPIAVDSNPDIRIVMYTRDSVPEFVKGQAGHIIDPAILDPETVAEELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVN6</accession>
    <name>A0A1U7EVN6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UspA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2034A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322737.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVN6"/>
    <dbReference type="STRING" id="348780.NP_2034A"/>
    <dbReference type="EnsemblBacteria" id="CAI49108">
        <property type="protein sequence ID" value="CAI49108"/>
        <property type="gene ID" value="NP_2034A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701333"/>
    <dbReference type="KEGG" id="nph:NP_2034A"/>
    <dbReference type="eggNOG" id="arCOG02053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_11_0_2"/>
    <dbReference type="OrthoDB" id="105697at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF6">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN UP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006276">
        <property type="entry name" value="UspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="166" mass="17081" checksum="31BAFCE3CD108CF9" modified="2017-05-10" version="1">MYDTILVPTDGGGGIGRVIDHAAEIAAAHGAALQFLYVLNTDSAAVSAAAVGGEAHELLRSQGEEALADADARVDVPTETALLEGQPADRIVEYADRTGCDLIVMGTHGRGGLGRLLLGSVAERVVRLSSQPVLTVHVDDSADNDKTSLEEVSPVTVDPAVSGVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVN8</accession>
    <name>A0A1U7EVN8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cytochrome c-type biogenesis protein CcdA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ccdA</name>
        <name evidence="8" type="ordered locus">NP_2040A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DsbD family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322740.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
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    <dbReference type="STRING" id="348780.NP_2040A"/>
    <dbReference type="EnsemblBacteria" id="CAI49111">
        <property type="protein sequence ID" value="CAI49111"/>
        <property type="gene ID" value="NP_2040A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702587"/>
    <dbReference type="KEGG" id="nph:NP_2040A"/>
    <dbReference type="eggNOG" id="arCOG02398">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="115386at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003834">
        <property type="entry name" value="Cyt_c_assmbl_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051790">
        <property type="entry name" value="Cytochrome_c-biogenesis_DsbD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31272:SF9">
        <property type="entry name" value="BLL1027 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31272">
        <property type="entry name" value="CYTOCHROME C-TYPE BIOGENESIS PROTEIN HI_1454-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DsbD_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
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    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="38"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome C biogenesis protein transmembrane" evidence="7">
        <location>
            <begin position="5"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="225" mass="22922" checksum="AF366AAB799B6321" modified="2017-05-10" version="1">MEVPTLSVVFIAGVATILTPCCLPLLPPLLSGSVGHRLRPIAIVAGSLVSFTALGVVVGTLGSFSPDSLRTPAFLAIVAFGAVMVDDDLNRIYSTYASRLSGWLTGSFSTLDADRRPLASAFSLGTLLGVIWLPCVGPVLGAVLAYAATTGAAAESGLLLFVYGTGFAVPLLGVAYGGKIAGRSVVSRLGAVGGTETLRRIAGGILLVTGVALLFDFDRVLLSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVS5</accession>
    <name>A0A1U7EVS5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2150A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322794.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVS5"/>
    <dbReference type="STRING" id="348780.NP_2150A"/>
    <dbReference type="EnsemblBacteria" id="CAI49166">
        <property type="protein sequence ID" value="CAI49166"/>
        <property type="gene ID" value="NP_2150A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701365"/>
    <dbReference type="KEGG" id="nph:NP_2150A"/>
    <dbReference type="eggNOG" id="arCOG14737">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_884593_0_0_2"/>
    <dbReference type="OrthoDB" id="242327at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="205"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="277"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="277"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="314" mass="33474" checksum="B0195B303D96295C" modified="2017-05-10" version="1">MATGSAAGDEPTLLGRLPSVRTAGQTLLRSLVFAPGRSAAVLLVALPYLWLGYYVVGVAAGRVGDPIIAGDWLTLMFTSYALVSSLALAHRILQTGRFGLSESYLVDVVVLVWLTTFYFVWMLAREAVSATTTVVELYAPVLHGEPTAVVWAAATVLTTAVAAGVVVHPREGSKLFAGEFRTALVTLPATVTALTLLVRPGGDSLLWPVLIGLFLGTLLGGLIRIHIVASAASKGVFAALSLFVWAFGTLLWVLIHRRRPPNWRVVLTHVAFGTTDRNADSTDRDTDGTIVQERQNRPGDTPAMSSDGGDPTRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVV9</accession>
    <name>A0A1U7EVV9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2196A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322817.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVV9"/>
    <dbReference type="STRING" id="348780.NP_2196A"/>
    <dbReference type="EnsemblBacteria" id="CAI49189">
        <property type="protein sequence ID" value="CAI49189"/>
        <property type="gene ID" value="NP_2196A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701375"/>
    <dbReference type="KEGG" id="nph:NP_2196A"/>
    <dbReference type="eggNOG" id="arCOG09024">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159740_0_0_2"/>
    <dbReference type="OrthoDB" id="306312at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058742">
        <property type="entry name" value="DUF7989"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25951">
        <property type="entry name" value="DUF7989"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="42"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="80" mass="8493" checksum="29348A2B043F1577" modified="2017-05-10" version="1">MTENTEPTTDDAETLGEMSHTNPYTGRVFGDTQTYSRGKTVAADGGTADAADAETLADVRHTPPEGSEGAQRAYDRGTNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVW8</accession>
    <name>A0A1U7EVW8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TRAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2242A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322839.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVW8"/>
    <dbReference type="STRING" id="348780.NP_2242A"/>
    <dbReference type="EnsemblBacteria" id="CAI49212">
        <property type="protein sequence ID" value="CAI49212"/>
        <property type="gene ID" value="NP_2242A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701389"/>
    <dbReference type="KEGG" id="nph:NP_2242A"/>
    <dbReference type="eggNOG" id="arCOG04655">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1976598_0_0_2"/>
    <dbReference type="OrthoDB" id="240517at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="126" mass="13765" checksum="0BC7C0B0716B404E" modified="2017-05-10" version="1">MVQSLVIGGVVALVVFVMLLGLFRRSRTPKTAAQKKRDAAHREAQQRPPEEAAEKGKTYELVVKETQYDRVPPEVRGTINGLQTFVRDIPDPGGSGALSVGDTIRVQVTDYGSNRTTAQARFIGRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW04</accession>
    <name>A0A1U7EW04_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NADH dehydrogenase-like complex subunit N</fullName>
            <ecNumber evidence="7">1.6.5.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">nuoN</name>
        <name evidence="7" type="ordered locus">NP_2314A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ND/Mrp_TM"/>
    </dbReference>
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        <property type="entry name" value="NDH_I_N"/>
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    </dbReference>
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        <property type="entry name" value="NADH DEHYDROGENASE"/>
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    </dbReference>
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        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
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            <begin position="6"/>
            <end position="24"/>
        </location>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="5">
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    <feature type="transmembrane region" description="Helical" evidence="5">
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="205"/>
            <end position="231"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="243"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="278"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="306"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="345"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="383"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="408"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="458"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:quinone oxidoreductase/Mrp antiporter transmembrane" evidence="6">
        <location>
            <begin position="126"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="495" mass="51304" checksum="096F60128A8D658E" modified="2017-05-10" version="1">MVSDWLLLAPPAAFITAALALFVVDAISSDETSGWLLSGVTTAGGLAAAAIAGVLLLSETAAGGVELFGGQLVVDGMSLLFTFIFGSVTALVAVASLDYIRDLPHKAEYYLLISLAATGMSLMASANSLVVVFISLELASLPSYALVAYLKNNRGSVEAGLKYFLIGALSSGIMLYGISLIYATTGSFLLGDIAASVGEAASTHPGVFGVGLLMVIGGFAFKTASVPFHFWAPDAYEGAPAPVAAFLSSASKAAGFVIAFRVLLVGFPIQEVAATVDWVLLFQILAVVTMTVGNFAAATQSNVKRMLAYSSIGHAGYVLIGLAALTNVGGPTAEATVLGASMLHLLVYGFMNTGAFLFVAMVEHWDVGRTFEDYNGLAREAPLACVAMTIFLFSLAGLPVGGGFLSKYYLVVGAVGTGVWWLAVVLAINSALSLYYYSRVVKAMWIESPAESLTIERYPTALYAAVMAAAAVTVLLLPGLFVFDGAAFDAAAALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW13</accession>
    <name>A0A1U7EW13_NATPD</name>
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        <recommendedName>
            <fullName evidence="4">Ribbon-helix-helix protein CopG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2330A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
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            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
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            <taxon>Natronomonas</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49256.1"/>
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    <dbReference type="STRING" id="348780.NP_2330A"/>
    <dbReference type="EnsemblBacteria" id="CAI49256">
        <property type="protein sequence ID" value="CAI49256"/>
        <property type="gene ID" value="NP_2330A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701408"/>
    <dbReference type="KEGG" id="nph:NP_2330A"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Met repressor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="226" mass="25430" checksum="ED065E9766B12921" modified="2017-05-10" version="1">MSKDIEQNPTRISVNPKSAFDVGELDDIADDKDMSRAALLREIIGDYVDEHTDDDGEVTNPDLAKPEHPDLRDAFMTLLDVSDHPRGPRRISVDEAEDALYSTDCAKSSVRRRYLEPLREDGYIAVKCGRITVRRRTVEEVERRQETADRQADTLAKSEPTNDDDRGDGPVMDELDKEHRKLIKSRRADIDPALDVIGWACENTVWDHGNQTAVRADGGEVTNDGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW16</accession>
    <name>A0A1U7EW16_NATPD</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
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        <name evidence="1" type="ordered locus">NP_2336A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
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    <dbReference type="STRING" id="348780.NP_2336A"/>
    <dbReference type="EnsemblBacteria" id="CAI49259">
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        <property type="gene ID" value="NP_2336A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701411"/>
    <dbReference type="KEGG" id="nph:NP_2336A"/>
    <dbReference type="HOGENOM" id="CLU_2821026_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="66" mass="6770" checksum="37C4618492FE5DAD" modified="2017-05-10" version="1">MSRNDVTGMALSAVLALSALSWTAYGLFGFDVFSLMGPEMTGGLAMIIGGLGAYDAADTFGIIDNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW69</accession>
    <name>A0A1U7EW69_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2488A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322961.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW69"/>
    <dbReference type="STRING" id="348780.NP_2488A"/>
    <dbReference type="EnsemblBacteria" id="CAI49335">
        <property type="protein sequence ID" value="CAI49335"/>
        <property type="gene ID" value="NP_2488A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701462"/>
    <dbReference type="KEGG" id="nph:NP_2488A"/>
    <dbReference type="eggNOG" id="arCOG03053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1072064_0_0_2"/>
    <dbReference type="OrthoDB" id="241821at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="242"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="259" mass="26450" checksum="FD6ACAE3BB49AAD8" modified="2017-05-10" version="1">MSGTALRVGVLAILLAGAIALGGFAAAPTAAQSVSENASVGAEISAFMQTTDAATERDVDDGMFETAFDRAEDRAEREALVEQRRQHLQDRFQRLADRRATLGAEPTVRNRSLAARIDAGASGIERSVNHTKRSAADAGVDAESLDELRANASRLRGPEVAELARGLAGPPDDRGPPGERGPSDGDSAGPGAGDRGPGTDEEARGGPAERGGPGGGSAAESTPDERSDADPDARDNHADFTPDGPSDGQPGDRPGGPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW99</accession>
    <name>A0A1U7EW99_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2538A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322985.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW99"/>
    <dbReference type="STRING" id="348780.NP_2538A"/>
    <dbReference type="EnsemblBacteria" id="CAI49360">
        <property type="protein sequence ID" value="CAI49360"/>
        <property type="gene ID" value="NP_2538A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702740"/>
    <dbReference type="KEGG" id="nph:NP_2538A"/>
    <dbReference type="eggNOG" id="arCOG00357">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037276_1_0_2"/>
    <dbReference type="OrthoDB" id="5875at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01899">
        <property type="entry name" value="Ygr210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013646">
        <property type="entry name" value="YGR210-like_G4"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007171">
        <property type="entry name" value="PRK09602.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305">
        <property type="entry name" value="OBG GTPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305:SF1">
        <property type="entry name" value="OBG-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08438">
        <property type="entry name" value="YGR210-like_G4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="2">
        <location>
            <begin position="3"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="395" mass="43479" checksum="90F09E063338EBC7" modified="2017-05-10" version="1">MSYTIGLVGKPSVGKSTFFNAATMNDVPEGAYPFTTIDPSMGEAYVRVECAAPEFDHSCTPNHGYCADGMRFVPTKLVDVAGLVPGAHEGKGLGNQFLTDLNEADVLIHVVDFTGETDLEGEPTEDHDPRDDIDFLENELDMWYLDILEKGIERYESGYHGTERDIEADLAEQMSAFGIGEDELKQVILALDLPLAPEPWDAADRERLAREIRIRTKPMVIAANKMDTEAAQENWEPITSDPDYEHLTFVPVSAHAEKALKQGDEQDVLSYRPGDDGFEITADLPDEKQAGLEQIREFIDDFGGTGVQSALETALFDELDAIAVFPGARKPQDDGTFLQDCFVLPGGSTAEEFAYFLHTDIGDGFLHAHDVRSGRQVGADTELSHRDVVEITTTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWD4</accession>
    <name>A0A1U7EWD4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Transducer protein Htr30</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">htr30</name>
        <name evidence="12" type="ordered locus">NP_2610A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWD4"/>
    <dbReference type="STRING" id="348780.NP_2610A"/>
    <dbReference type="EnsemblBacteria" id="CAI49396">
        <property type="protein sequence ID" value="CAI49396"/>
        <property type="gene ID" value="NP_2610A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_2610A"/>
    <dbReference type="eggNOG" id="arCOG02318">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_116_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11386">
        <property type="entry name" value="MCP_signal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.950">
        <property type="entry name" value="Methyl-accepting chemotaxis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004090">
        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32089:SF112">
        <property type="entry name" value="LYSOZYME-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32089">
        <property type="entry name" value="METHYL-ACCEPTING CHEMOTAXIS PROTEIN MCPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00260">
        <property type="entry name" value="CHEMTRNSDUCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58104">
        <property type="entry name" value="Methyl-accepting chemotaxis protein (MCP) signaling domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="PAC" evidence="9">
        <location>
            <begin position="233"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="11">
        <location>
            <begin position="294"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="10">
        <location>
            <begin position="341"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="8">
        <location>
            <begin position="350"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="9"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="270"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="386"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI49396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="621" mass="66125" checksum="887DD7B1873FABF6" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWH5</accession>
    <name>A0A1U7EWH5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7992 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2696A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49439.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939545.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWH5"/>
    <dbReference type="EnsemblBacteria" id="CAI49439">
        <property type="protein sequence ID" value="CAI49439"/>
        <property type="gene ID" value="NP_2696A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701528"/>
    <dbReference type="KEGG" id="nph:NP_2696A"/>
    <dbReference type="eggNOG" id="arCOG04577">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142036_0_0_2"/>
    <dbReference type="OrthoDB" id="165952at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058305">
        <property type="entry name" value="DUF7992"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25955">
        <property type="entry name" value="DUF7992"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7992" evidence="2">
        <location>
            <begin position="3"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49439.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="150" mass="17366" checksum="68BB31F23D5951AB" modified="2017-05-10" version="1">MGLDVTPPDPPALPEQDTDEYEDVDVQGTDYRRDELETFLHNGAWEEAFERWAAETDLEERAYDIADDLGMFEAFDFFWDDFADRVGYHAPGIPEDWKERELHPELSSWETVSGINAAMAEFGDVVSRTLKEEYVDWEADYDAPDDLPDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX42</accession>
    <name>A0A1U7EX42_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">HAD superfamily hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_3182A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HAD-like hydrolase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323304.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX42"/>
    <dbReference type="STRING" id="348780.NP_3182A"/>
    <dbReference type="EnsemblBacteria" id="CAI49682">
        <property type="protein sequence ID" value="CAI49682"/>
        <property type="gene ID" value="NP_3182A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701679"/>
    <dbReference type="KEGG" id="nph:NP_3182A"/>
    <dbReference type="eggNOG" id="arCOG02291">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_8_2_2"/>
    <dbReference type="OrthoDB" id="131325at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050124">
        <property type="term" value="F:N-acylneuraminate-9-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046380">
        <property type="term" value="P:N-acetylneuraminate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051400">
        <property type="entry name" value="HAD-like_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46470">
        <property type="entry name" value="N-ACYLNEURAMINATE-9-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46470:SF3">
        <property type="entry name" value="N-ACYLNEURAMINATE-9-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="218" mass="23151" checksum="64763C92ADB79A2B" modified="2017-05-10" version="1">MTVGYLFDFDRTLVTYEPDIPGIFRNACEAVGVEPPDDAADIIRTGYVETFCAFDESPYLGAARAARDAGLDVDPERLAEAHIEAELAATTVPDGVGDLLASLSSVGIVTNGYGPVQRRKLAETGLEPHVDAVVCADDVEAFKPDNAPFDAITAALPAGDYVMVGDSVEYDIRPAAERGYRTVYVGDGDAAEADICLSSPAHLPALPRRLDAVEPTDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX49</accession>
    <name>A0A1U7EX49_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">KEOPS complex Pcc1-like subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3208A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323317.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX49"/>
    <dbReference type="STRING" id="348780.NP_3208A"/>
    <dbReference type="EnsemblBacteria" id="CAI49695">
        <property type="protein sequence ID" value="CAI49695"/>
        <property type="gene ID" value="NP_3208A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701684"/>
    <dbReference type="KEGG" id="nph:NP_3208A"/>
    <dbReference type="eggNOG" id="arCOG01354">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_169408_1_0_2"/>
    <dbReference type="OrthoDB" id="107316at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011470">
        <property type="entry name" value="PRK14887.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="75" mass="8136" checksum="BB260330A93B9D4D" modified="2017-05-10" version="1">MTQRRLTIRTDHDRPEVVAAAVAADNTAELSTHAEDGTVETTIERETTGGLRTTADDYVCNLIVAQQTTDTTTQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX61</accession>
    <name>A0A1U7EX61_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">Methionine aminopeptidase</fullName>
            <shortName evidence="8">MAP</shortName>
            <shortName evidence="8">MetAP</shortName>
            <ecNumber evidence="8 9">3.4.11.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Peptidase M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 11" type="primary">map</name>
        <name evidence="11" type="ordered locus">NP_3190A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8 9">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="8">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the peptidase M24A family. Methionine aminopeptidase archaeal type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="8 9"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323308.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX61"/>
    <dbReference type="STRING" id="348780.NP_3190A"/>
    <dbReference type="EnsemblBacteria" id="CAI49686">
        <property type="protein sequence ID" value="CAI49686"/>
        <property type="gene ID" value="NP_3190A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702897"/>
    <dbReference type="KEGG" id="nph:NP_3190A"/>
    <dbReference type="eggNOG" id="arCOG01001">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015857_7_0_2"/>
    <dbReference type="OrthoDB" id="372008at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004239">
        <property type="term" value="F:initiator methionyl aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01975">
        <property type="entry name" value="MetAP_2_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050247">
        <property type="entry name" value="Met_Aminopeptidase_Type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028595">
        <property type="entry name" value="MetAP_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002468">
        <property type="entry name" value="Pept_M24A_MAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00501">
        <property type="entry name" value="met_pdase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45777">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45777:SF2">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 9" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="7 8 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M24" evidence="10">
        <location>
            <begin position="11"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI49686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="297" mass="32365" checksum="A2E1F9EBD3A87ED5" modified="2017-05-10" version="1">MSVDLSDEKFEKHREAGEILAQVREEAAERVEVGVSHLDVAEYAEERIRELGGEPAFPVNISIDEEAAHATPAPDDESEFGEEMVNLDIGVHVDGWLADTAVTVDLSGNAELAEASAEALEAAIDVVEPGVHTGEIGAEIESVIDGYGYNPVVNLSGHGLGHWEQHTEPNIPNRGVQQGVELEVGDVVAIEPFATDGGGKVSEGATEEIYALEREASVRDRGARQALEQIVEEFKTLPFARRWLETSRAEMALRRLKQKNVVHGYPVLKEEDGCLVSQKEHTVLVTEDGCEVTTRPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXD0</accession>
    <name>A0A1U7EXD0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">HTH/CBS domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cbs8</name>
        <name evidence="5" type="ordered locus">NP_3466A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323444.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXD0"/>
    <dbReference type="STRING" id="348780.NP_3466A"/>
    <dbReference type="EnsemblBacteria" id="CAI49824">
        <property type="protein sequence ID" value="CAI49824"/>
        <property type="gene ID" value="NP_3466A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701780"/>
    <dbReference type="KEGG" id="nph:NP_3466A"/>
    <dbReference type="eggNOG" id="arCOG00608">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116133_0_0_2"/>
    <dbReference type="OrthoDB" id="30763at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017158">
        <property type="entry name" value="Tscrpt-reg_CBS-contain_prd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF4">
        <property type="entry name" value="CRO-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037253">
        <property type="entry name" value="HTH_CBS_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="3">
        <location>
            <begin position="9"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="70"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="180" mass="19416" checksum="B58B754D536F86AC" modified="2017-05-10" version="1">MDLPTPADLKERRTELELTQSELADYADVSQPLIARIEGGDVDPRLSTLRRIVNALDEAESAVMHAADIMHEGVVSVAPDDSVREAIDVMVTEGYSQLPVIRDGRPQGIISNSDIRQLDSESAGDLPVAEAMREAITTVEPDATLDEVNAHLNHQDAVMVVEDGQLQGIVTEADVAAHIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXH2</accession>
    <name>A0A1U7EXH2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cupin 2 barrel domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3534A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323478.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXH2"/>
    <dbReference type="STRING" id="348780.NP_3534A"/>
    <dbReference type="EnsemblBacteria" id="CAI49858">
        <property type="protein sequence ID" value="CAI49858"/>
        <property type="gene ID" value="NP_3534A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701798"/>
    <dbReference type="KEGG" id="nph:NP_3534A"/>
    <dbReference type="eggNOG" id="arCOG03008">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149791_1_0_2"/>
    <dbReference type="OrthoDB" id="199885at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051610">
        <property type="entry name" value="GPI/OXD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35848:SF6">
        <property type="entry name" value="CUPIN TYPE-2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35848">
        <property type="entry name" value="OXALATE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin type-2" evidence="2">
        <location>
            <begin position="46"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="104" mass="11265" checksum="61215A14196B85E9" modified="2017-05-10" version="1">MTHVPRAELLQQLDGADSDSARVVDTESMTVGLKRYREGPANEKGLREHTDDELYYILDGSGTIAVGDAVHSVAPGDVLYVEKGTSHDVQEVDEALTVLKVLAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXI9</accession>
    <name>A0A1U7EXI9_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAD-dependent urate hydroxylase HpyO/Asp monooxygenase CreE-like FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3570A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49876.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_198408086.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXI9"/>
    <dbReference type="STRING" id="348780.NP_3570A"/>
    <dbReference type="EnsemblBacteria" id="CAI49876">
        <property type="protein sequence ID" value="CAI49876"/>
        <property type="gene ID" value="NP_3570A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701811"/>
    <dbReference type="KEGG" id="nph:NP_3570A"/>
    <dbReference type="eggNOG" id="arCOG11370">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014633_2_0_2"/>
    <dbReference type="OrthoDB" id="201607at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038732">
        <property type="entry name" value="HpyO/CreE_NAD-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38663">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38663:SF1">
        <property type="entry name" value="L-ORNITHINE N(5)-MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13454">
        <property type="entry name" value="NAD_binding_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-dependent urate hydroxylase HpyO/Asp monooxygenase CreE-like FAD/NAD(P)-binding" evidence="1">
        <location>
            <begin position="14"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49876.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="415" mass="44410" checksum="94A0075BEF8F665B" modified="2017-05-10" version="1">MTDTSMPDEQAVTIVGSGVHGVHLAIRLLSDDVVSRDRLCLVDPSGLLASFRRKSRACGMASLRSPFVHQLDADPFSLRDFAQKHGREDELVASEDGADRPTTELFFDHADRLCEQHGLESLRVDAAVTGLSDHGAHVRVETTAGSFSSAWCVLAVGYGGSYSMPAWADGLPSSAPVAHVWDGCFEPSAVGDSERLGIIGSGVTGVQLAASLACPERDVVVFARSPFRTQRREAGTDWMHFSDALEALHSYPPASRGRERVVADARYDGSVPPYAMARFRRAVDRGWITVEATEVVDATAAGGTVVVTCRDGRADWFDRLICATGFGTPYEGTLLSRLRNETSLATGYLGAPALDDDSLRWRRADGTRSRVAVSGAAAQQVLGPFARNIVGARQAGNILADVIETEPAAPPTIRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXJ7</accession>
    <name>A0A1U7EXJ7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC-type transport system permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">abc24p</name>
        <name evidence="9" type="ordered locus">NP_3644A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AzlC family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323531.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXJ7"/>
    <dbReference type="STRING" id="348780.NP_3644A"/>
    <dbReference type="EnsemblBacteria" id="CAI49913">
        <property type="protein sequence ID" value="CAI49913"/>
        <property type="gene ID" value="NP_3644A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702441"/>
    <dbReference type="KEGG" id="nph:NP_3644A"/>
    <dbReference type="eggNOG" id="arCOG04452">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065777_2_1_2"/>
    <dbReference type="OrthoDB" id="170360at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903785">
        <property type="term" value="P:L-valine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011606">
        <property type="entry name" value="Brnchd-chn_aa_trnsp_permease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34979">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YGAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34979:SF1">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YGAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03591">
        <property type="entry name" value="AzlC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="132"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="164"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI49913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="227" mass="23399" checksum="787C3F617E061ECB" modified="2017-05-10" version="1">MAVSFERDGIVAGFRTGLPVAIGVGAYGIAFGVLARQSGLSVAEATLMSATVVAGASQLVAVELWGTPLPVVTILVTTLVINLRYVLMGASLRPWFKSLTPAQAYGSSFFITDETWALSIADLRSETGRGAFLLGAGLALWSLWVLTTAIGAAAGSAVTDPERFGLDFVLTAIFVILAVGLWRGRNDTAPWAAAAAVAVAAEAAAPGQWYILFGGLAGSLVAVVQRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXJ8</accession>
    <name>A0A1U7EXJ8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Probable RfbX family transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tp56</name>
        <name evidence="7" type="ordered locus">NP_3624A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323523.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXJ8"/>
    <dbReference type="STRING" id="348780.NP_3624A"/>
    <dbReference type="EnsemblBacteria" id="CAI49903">
        <property type="protein sequence ID" value="CAI49903"/>
        <property type="gene ID" value="NP_3624A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703345"/>
    <dbReference type="KEGG" id="nph:NP_3624A"/>
    <dbReference type="eggNOG" id="arCOG02209">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022017_5_1_2"/>
    <dbReference type="OrthoDB" id="19148at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd13128">
        <property type="entry name" value="MATE_Wzx_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050833">
        <property type="entry name" value="Poly_Biosynth_Transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002797">
        <property type="entry name" value="Polysacc_synth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30250:SF27">
        <property type="entry name" value="POLYSACCHARIDE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30250">
        <property type="entry name" value="PST FAMILY PREDICTED COLANIC ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01943">
        <property type="entry name" value="Polysacc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="90"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="166"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="253"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="312"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="342"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="383"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="436"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="463"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="505" mass="53830" checksum="28715D3B8C70E6D7" modified="2017-05-10" version="1">MSEQRKSRRASLATIVRGGSLYTVGKVVSDVGEFLLHLLVSRWLGAGLYGLFAYGKTLVFTALLLTNLGSDKSIVRYLPKYESNPEKRRFVLALAWVTSLGGGFAVASGLFVFAPTVAGVTLDQPGFVPVLRLFAVVLFVDTAANLLYATFRALEVIEYEVLSKRLLKPVLRVLAVGSAVLIGGTIYSVVVAMVVASVATLAVAGYLFVTRLEIRPRLRSPAGTRETVRDYYNYSLPLTAKDAGTVMQSRVDVLMVGVFLSSTAVGVYNVSVLLASVLYVPLLAANQLFAPVISRLQSQGRTADMQAIYRAVTRWIFTASLFFAAVLVVFRVELLGLFGPEFTAGTTVLVLFVGAQLCNAAVGPSGDLLMMTDHQYAVMLNEWVFGVANVVLTVVFIQFFGFVGAALASAGVLLARNLTKLTEVWYLERMQPYGRAFGKPLAAGGVAAAGMVGTHAATAAAGFGAWTGVAVGVAVGIGLYAGGLWMAGFEEVDYELYAQLVGSGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXK1</accession>
    <name>A0A1U7EXK1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glyoxalase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3594A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323508.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXK1"/>
    <dbReference type="STRING" id="348780.NP_3594A"/>
    <dbReference type="EnsemblBacteria" id="CAI49888">
        <property type="protein sequence ID" value="CAI49888"/>
        <property type="gene ID" value="NP_3594A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701817"/>
    <dbReference type="KEGG" id="nph:NP_3594A"/>
    <dbReference type="eggNOG" id="arCOG02708">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1912402_0_0_2"/>
    <dbReference type="OrthoDB" id="6111at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004493">
        <property type="term" value="F:methylmalonyl-CoA epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046491">
        <property type="term" value="P:L-methylmalonyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051785">
        <property type="entry name" value="MMCE/EMCE_epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43048">
        <property type="entry name" value="METHYLMALONYL-COA EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43048:SF3">
        <property type="entry name" value="METHYLMALONYL-COA EPIMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="2">
        <location>
            <begin position="5"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="132" mass="14411" checksum="3FCA6E27F03C3B91" modified="2017-05-10" version="1">MTPESFFHIALKVDDVDEGLAFYRQHFDAELIERGHADDGDGATAVNHVAVAVADKRVYLFDRAPYEAAGLVDERPTGFLHFGYVVDDVEAACADLAASGVEFIMEPSVFGDLKIAFVTDPAGVRIELLEHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXK4</accession>
    <name>A0A1U7EXK4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Small CPxCG-related zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3688A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323553.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXK4"/>
    <dbReference type="STRING" id="348780.NP_3688A"/>
    <dbReference type="EnsemblBacteria" id="CAI49935">
        <property type="protein sequence ID" value="CAI49935"/>
        <property type="gene ID" value="NP_3688A"/>
    </dbReference>
    <dbReference type="GeneID" id="32155014"/>
    <dbReference type="KEGG" id="nph:NP_3688A"/>
    <dbReference type="eggNOG" id="arCOG01989">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_196471_0_0_2"/>
    <dbReference type="OrthoDB" id="35104at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044720">
        <property type="entry name" value="HVO_2753-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049683">
        <property type="entry name" value="HVO_2753-like_euryarch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011668">
        <property type="entry name" value="HVO_2753-like_ZBP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041909">
        <property type="entry name" value="HVO_2753"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011481">
        <property type="entry name" value="PRK14890.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40733:SF1">
        <property type="entry name" value="SMALL ZINC FINGER PROTEIN HVO-2753-LIKE ZINC-BINDING POCKET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40733">
        <property type="entry name" value="ZINC-RIBBON RNA-BINDING PROTEIN INVOLVED IN TRANSLATION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07754">
        <property type="entry name" value="HVO_2753_ZBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Small zinc finger protein HVO-2753-like zinc-binding pocket" evidence="1">
        <location>
            <begin position="13"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="60" mass="6531" checksum="FFE2F315B3B16F5C" modified="2017-05-10" version="1">MSSTSDKRRDHSCISCGISISGTDAAKFDCPECGTLIYRCSKCRKQSNLYECPDCGFTGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXL2</accession>
    <name>A0A1U7EXL2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DUF2078 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_3614A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323518.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXL2"/>
    <dbReference type="EnsemblBacteria" id="CAI49898">
        <property type="protein sequence ID" value="CAI49898"/>
        <property type="gene ID" value="NP_3614A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701824"/>
    <dbReference type="KEGG" id="nph:NP_3614A"/>
    <dbReference type="eggNOG" id="arCOG03912">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1901997_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018649">
        <property type="entry name" value="SHOCT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09851">
        <property type="entry name" value="SHOCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="SHOCT" evidence="3">
        <location>
            <begin position="75"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="133" mass="14813" checksum="4AD58ECA8137DB26" modified="2017-05-10" version="1">MDEEDTEPLVTHEMLGYMLGFGLDFILLAGLLWVVGVVPGVVFAGVTGFFAVVYTLWIAWRWHSRRRDDSADREPVDALKERYVAGELSEAEFEAELSRLMDAGPDADGREQKTSDRTAAETASDRQEAAESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXM2</accession>
    <name>A0A1U7EXM2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">tRNA-specific adenosine deaminase</fullName>
            <ecNumber evidence="1">3.5.4.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3680A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.4.-" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323549.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXM2"/>
    <dbReference type="STRING" id="348780.NP_3680A"/>
    <dbReference type="EnsemblBacteria" id="CAI49931">
        <property type="protein sequence ID" value="CAI49931"/>
        <property type="gene ID" value="NP_3680A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701838"/>
    <dbReference type="KEGG" id="nph:NP_3680A"/>
    <dbReference type="eggNOG" id="arCOG04130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076814_1_0_2"/>
    <dbReference type="OrthoDB" id="7902at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.280">
        <property type="entry name" value="AF1531-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007003">
        <property type="entry name" value="DUF655"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40734:SF1">
        <property type="entry name" value="DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40734">
        <property type="entry name" value="TRNA-SPECIFIC ADENOSINE DEAMINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04919">
        <property type="entry name" value="DUF655"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160975">
        <property type="entry name" value="AF1531-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="191" mass="21587" checksum="590D4312FD36A92B" modified="2017-05-10" version="1">MSDDGDDTAARTAVVLDFLPRGSPSDDRPQYEKSPVAFAVGEEDFQLVEAALTDDAGINIGDRIEIDPVGDNVKDLRTVEYRDLTSTAESELEYAIESIIDADEAQFVDFYNDAQPITTRLHVLNLLPGIGKKLRNNVLDARKRQPFEDFDDIEERVSGLHDPKGVLIERITEELQEDDLKYRIFARRDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXN7</accession>
    <name>A0A1U7EXN7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Zinc-containing ferredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ferB</name>
        <name evidence="10" type="ordered locus">NP_3692A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6 7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6 7">Binds 1 zinc ion.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6 7">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
        <text evidence="6 7">Binds 1 [3Fe-4S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6 7">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6 7">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323555.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXN7"/>
    <dbReference type="STRING" id="348780.NP_3692A"/>
    <dbReference type="EnsemblBacteria" id="CAI49937">
        <property type="protein sequence ID" value="CAI49937"/>
        <property type="gene ID" value="NP_3692A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702706"/>
    <dbReference type="KEGG" id="nph:NP_3692A"/>
    <dbReference type="eggNOG" id="arCOG04548">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_178207_0_0_2"/>
    <dbReference type="OrthoDB" id="23833at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
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    </dbReference>
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        <location>
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            <end position="66"/>
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            <end position="104"/>
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        <location>
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            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
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        <location>
            <position position="46"/>
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        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
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        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
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        </ligand>
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        <location>
            <position position="56"/>
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        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
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        <location>
            <position position="84"/>
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        <ligand>
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            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
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            <position position="90"/>
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            <name>[4Fe-4S] cluster</name>
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            <position position="94"/>
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        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR000068-1"/>
        </source>
    </evidence>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI49937.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="109" mass="12046" checksum="8BF3A6FFB68FCF01" modified="2017-05-10" version="1">MAIDPNFEETREREGQERGVDVWGPTDPPEQLGIHGTHVAVDLDICVGDGACLEDCPVDVFEWIDTPDHPESEQKVTPADEDQCIDCMLCVDVCPVDAIDVDPGRENRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXV1</accession>
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        <name evidence="6" type="ordered locus">NP_3876A</name>
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        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
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        <property type="entry name" value="ATP-DEPENDENT HELICASE HRQ1"/>
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        <location>
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            <end position="308"/>
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        <location>
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            <end position="1127"/>
        </location>
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        <source>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI50029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="1767" mass="200663" checksum="48FAE6AE96A6CE25" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXZ2</accession>
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        <submittedName>
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        </submittedName>
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        <name evidence="1" type="primary">phr4</name>
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    </gene>
    <organism evidence="1 2">
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            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
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            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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    <dbReference type="EnsemblBacteria" id="CAI50069">
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        <property type="gene ID" value="NP_3956A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701921"/>
    <dbReference type="KEGG" id="nph:NP_3956A"/>
    <dbReference type="eggNOG" id="arCOG02839">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031632_1_0_2"/>
    <dbReference type="OrthoDB" id="371734at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Deoxyribodipyrimidine photolyase-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.579.10">
        <property type="entry name" value="DNA Cyclobutane Dipyrimidine Photolyase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Crypto/Photolyase_FAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007357">
        <property type="entry name" value="PhrB-like"/>
    </dbReference>
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        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052551">
        <property type="entry name" value="UV-DNA_repair_photolyase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38657">
        <property type="entry name" value="SLR1343 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38657:SF1">
        <property type="entry name" value="SLR1343 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04244">
        <property type="entry name" value="DPRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48173">
        <property type="entry name" value="Cryptochrome/photolyase FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="511" mass="57727" checksum="81084D42D2A0CD63" modified="2017-05-10" version="1">MTVLVLGDCLTEFGPLASDARSTDERVLCIEARAFARRKPYHPHKLTLVFSAMRHFRDRLREAGYTVDYRRVETFAEGLDAHFAAHPEDHIVTVRRTAHGATDRLQRLVANRGGTVEFVADPRFHCSREEFDAWADGDPPYRHESFYRHMRRETGYLMDGDEPVGGEWNFDDENREFPGPEYVPPEPPQFEPDETTREVREWVDATFGEDGYDDAPYGGAWADPEPFSWPVTREGALQALEAFIEERLPTFGPYQDAMLGDEWAMNHALLSSSLNLGLLSPSEVIEAALAAFEEGSVSIASVEGFLRQVLGWREFVRHAYRRTPGMAAANQLGAAEPLPEFFWTGDTDMACVADAVDGVRTRGYAHHIERLMVLSNFATLYGVEPSRLNEWFHAAFVDAYHWVTTPNVVGMGTFGTDTLSTKPYVASANYIDRMSDHCSGCPYYKTKTTGDGACPFNALYWDFLGRNESQLRSNHRMGLVYSHYDDKSDGEREAIADRAETLRQRARNGTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXZ6</accession>
    <name>A0A1U7EXZ6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Rpa-associated phosphoesterase</fullName>
            <ecNumber evidence="2">3.1.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rpe</name>
        <name evidence="2" type="ordered locus">NP_3964A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.-.-" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323689.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXZ6"/>
    <dbReference type="STRING" id="348780.NP_3964A"/>
    <dbReference type="EnsemblBacteria" id="CAI50073">
        <property type="protein sequence ID" value="CAI50073"/>
        <property type="gene ID" value="NP_3964A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701924"/>
    <dbReference type="KEGG" id="nph:NP_3964A"/>
    <dbReference type="eggNOG" id="arCOG01150">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075478_0_0_2"/>
    <dbReference type="OrthoDB" id="10013at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07391">
        <property type="entry name" value="MPP_PF1019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024173">
        <property type="entry name" value="Pesterase_MJ0037-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39323">
        <property type="entry name" value="BLR1149 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39323:SF1">
        <property type="entry name" value="BLR1149 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000887">
        <property type="entry name" value="Pesterase_MJ0037"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="1">
        <location>
            <begin position="19"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="244" mass="26175" checksum="6B22C0B9D52F454B" modified="2017-05-10" version="1">MLEPVFGEPLAVADCGPERVLVVADYHAGIEVGLRADGVELRSAAEKRRKRLLSAIESELVDRCLFLGDLGDSIGSPSRTERAELRELLSAVTDHVPVTVTKGNHDGGIESITEAFDAVNVVDGRGTTIGSVGFCHGHTWPSASVIGADVVVVGHEHPLVRLEDEVGGGRTERVWLRGRIDPAGFPDNDTVGDRLVVCPAFNERSGGTRINERDEFLSPFLPDGLADGDAYLLDGTRLGPYRDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY05</accession>
    <name>A0A1U7EY05_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GNAT family acetyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3988A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY05"/>
    <dbReference type="STRING" id="348780.NP_3988A"/>
    <dbReference type="EnsemblBacteria" id="CAI50085">
        <property type="protein sequence ID" value="CAI50085"/>
        <property type="gene ID" value="NP_3988A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3988A"/>
    <dbReference type="eggNOG" id="arCOG00848">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102053_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051556">
        <property type="entry name" value="N-term/lysine_N-AcTrnsfr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42919:SF35">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42919">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="7"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="165" mass="18819" checksum="4B0A8F92FB10E55F" modified="2017-05-10" version="1">MAVSVTIDTRTIGAGDDALVESAWELKERIRRAEGVLKQRRDFFTDAYRRARVYALVEPGYDDEQLVGFAATRRDGYILFLAVDPNYRDRGFGSQLVAAVADDHSSVTCHARTTNEPALDFYRSLGFEIERRIDDYYEDHGDAYYLRLGENESLTTKLSRFMPGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYI3</accession>
    <name>A0A1U7EYI3_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">LppX domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4390A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYI3"/>
    <dbReference type="STRING" id="348780.NP_4390A"/>
    <dbReference type="EnsemblBacteria" id="CAI50286">
        <property type="protein sequence ID" value="CAI50286"/>
        <property type="gene ID" value="NP_4390A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_4390A"/>
    <dbReference type="eggNOG" id="arCOG06249">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1109524_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.50.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="246" mass="26267" checksum="2D22A4FF3D37F947" modified="2017-05-10" version="1">MLDTAVERVHVVLAVVGCLLLAGCAGFGGTEGGTETESPSVNATALAANTDAVETYSVEITRTLQSTGANQTVEVDGAVDRENEAARLVQTTETAFRDEPRRTEQYVHEGTLYTSSDGEWEQSELEDWTTVDHLASAIPGPLASDDLEPIRTETVAGTETTMYEANITRGQAADLAGVDGTGHVPMTIEDYVYYVLVDDETDTLYGADLRLEVSQGGDPAFVTIETTITDRDGPVDVELPEEATAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYJ2</accession>
    <name>A0A1U7EYJ2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glyoxalase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4460A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323936.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYJ2"/>
    <dbReference type="STRING" id="348780.NP_4460A"/>
    <dbReference type="EnsemblBacteria" id="CAI50321">
        <property type="protein sequence ID" value="CAI50321"/>
        <property type="gene ID" value="NP_4460A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702758"/>
    <dbReference type="KEGG" id="nph:NP_4460A"/>
    <dbReference type="eggNOG" id="arCOG02708">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1052141_0_0_2"/>
    <dbReference type="OrthoDB" id="134577at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004462">
        <property type="term" value="F:lactoylglutathione lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019243">
        <property type="term" value="P:methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46036">
        <property type="entry name" value="LACTOYLGLUTATHIONE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46036:SF5">
        <property type="entry name" value="LACTOYLGLUTATHIONE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="4"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="130"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="251" mass="28720" checksum="1EBAE72DE7E34F8E" modified="2017-05-10" version="1">MDGILDHTMIRVEDLDASLEWYTTHLNYEEKGRWEAETFTNVYLGPEDAGDAAARLELTYNHDGRSYTMGDAWGHIAVRVPDVEAAYDELMQSGVEDYRPPAENPGYAFVTDPDGHEVEIVERDYGTKWSLDHTMLRVEDVNAALGYWIRTFEYEPTGRWEADSFANYFVSPQDAPAEAMDIELTYNYDGRSYTMGDAWGHVAVRCADLQSAWETLQEREAPDYRDPESCDNRYAFTKAPDGHEIEILPTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYK2</accession>
    <name>A0A1U7EYK2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4482A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323947.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYK2"/>
    <dbReference type="STRING" id="348780.NP_4482A"/>
    <dbReference type="EnsemblBacteria" id="CAI50332">
        <property type="protein sequence ID" value="CAI50332"/>
        <property type="gene ID" value="NP_4482A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702075"/>
    <dbReference type="KEGG" id="nph:NP_4482A"/>
    <dbReference type="eggNOG" id="arCOG04759">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130786_0_0_2"/>
    <dbReference type="OrthoDB" id="201266at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043835">
        <property type="entry name" value="DUF5811"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19128">
        <property type="entry name" value="DUF5811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="109" mass="11102" checksum="70090441B3E2AD51" modified="2017-05-10" version="1">MHGNTPYAGAPAADAEPTPEHRRALRRELVSVAASTRDLLSDEFVVGGEVADGGDGSLRATIAIRPPAGGVVSAGLDPEDNTETVARKLAAGAVFEAKRAGRDVKPTAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYN0</accession>
    <name>A0A1U7EYN0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4518A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323965.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYN0"/>
    <dbReference type="EnsemblBacteria" id="CAI50350">
        <property type="protein sequence ID" value="CAI50350"/>
        <property type="gene ID" value="NP_4518A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702087"/>
    <dbReference type="KEGG" id="nph:NP_4518A"/>
    <dbReference type="eggNOG" id="arCOG04774">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1302596_0_0_2"/>
    <dbReference type="OrthoDB" id="192229at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046147">
        <property type="entry name" value="DUF6149"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19646">
        <property type="entry name" value="DUF6149"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="199" mass="22199" checksum="3E04273F098A28CD" modified="2017-05-10" version="1">MKLYQNPRHWASKQALTTPGLRGAANYALVKLHTKIFLGKADADRREERRDHLDDLFDATIDAYVAALEADYTEAEAREITHIQANFDFFNHGWTEMMEIPADELEPHYRRYESFFQEHGITIDDPLGEFRPAGGLTSAPETPEKLDEPAFENAIAGFADDVYVETADGETVVGGDTEPPEDVDPTAVPGVDDEEAQAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYN1</accession>
    <name>A0A1U7EYN1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF7847 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_4512A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323962.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYN1"/>
    <dbReference type="STRING" id="348780.NP_4512A"/>
    <dbReference type="EnsemblBacteria" id="CAI50347">
        <property type="protein sequence ID" value="CAI50347"/>
        <property type="gene ID" value="NP_4512A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702084"/>
    <dbReference type="KEGG" id="nph:NP_4512A"/>
    <dbReference type="eggNOG" id="arCOG03934">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084120_0_0_2"/>
    <dbReference type="OrthoDB" id="205869at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057169">
        <property type="entry name" value="DUF7847"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25231">
        <property type="entry name" value="DUF7847"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="152"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="208"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="238"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7847" evidence="3">
        <location>
            <begin position="3"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="269"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="296" mass="31157" checksum="0833D71735F38270" modified="2017-05-10" version="1">MSLDIGQALREGASRTVEKNGLTLAAVFAGIALLTTVLFQTLTVGIAEAMLDFVQELSAEEMQEAGMSEAEFEEMTAELEAAAEEFRDTAPLALGLPVGAAAGGLLVLALVHEAARLIAVRVFATDSSDEVTSDLVTDNILLATLNGFIGGIVVWGLIIVGLVFIIIPGIILAVLFYFLRQEIALNDKNFVQAMADSWRITKGHRIEVFLIGFIVVLVSRLEEISSTAVGVVSTPAGAVAAAIVGGLLAAFGTAVVTRAYVQINDDDASDADVDETTDDIEDDPYDAALGPDDLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYQ4</accession>
    <name>A0A1U7EYQ4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">IS1341-type transposase ISNph28</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">NP_4546A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323979.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYQ4"/>
    <dbReference type="STRING" id="348780.NP_4546A"/>
    <dbReference type="EnsemblBacteria" id="CAI50364">
        <property type="protein sequence ID" value="CAI50364"/>
        <property type="gene ID" value="NP_4546A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702095"/>
    <dbReference type="KEGG" id="nph:NP_4546A"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_3_3_2"/>
    <dbReference type="OrthoDB" id="210698at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF26">
        <property type="entry name" value="TRANSPOSASE, PROBABLY IS605-TNPB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="7">
        <location>
            <begin position="179"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="8">
        <location>
            <begin position="297"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="389"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI50364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="414" mass="47090" checksum="D7ECF18FC8A5DB8D" modified="2017-05-10" version="1">MEKRRTVPVKLDVDSHSAALLEDTVDEFLWAANYTTRHAFKGEYVTTSKTTLHDDTYEDVRDQTALHSNHVQAARNKAAEACKSVVARWKNGKKASMPHFTSPHLVYDHRTATFHDEYVSLATVDGRIETDYVLPDKTRDTPHTDYFFSDEYETAGAELHYTNGNWMLHIHCKTDVESNTPEQATPENGTVLGVDLGVNNLAVASTGTFWTGDEFDHWRREYEKRRGDLQECGTRWAHENMQAVGRKEEGRFKQTLHRISNELVAEAHKYECSVIAFEELTDIRERTGASWGHKWAFNRLYEYVEYKTKEYGITVAQVDPENTSRRCSTCGFTHPDNRESESFECLKCGYENHADYNAAKNIGLRYLRRNQTGGDEGAPVGVRLNSGMLNANGEYESPADDSARAGVHAESQRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYT3</accession>
    <name>A0A1U7EYT3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_4648A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYT3"/>
    <dbReference type="STRING" id="348780.NP_4648A"/>
    <dbReference type="EnsemblBacteria" id="CAI50415">
        <property type="protein sequence ID" value="CAI50415"/>
        <property type="gene ID" value="NP_4648A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_4648A"/>
    <dbReference type="eggNOG" id="arCOG09115">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_504927_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055941">
        <property type="entry name" value="DUF7519"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24363">
        <property type="entry name" value="DUF7519"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="25"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="49"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="234"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="282"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="322"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="387"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="423"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="482"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="515"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="539" mass="54442" checksum="90EFF270824B78C3" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYT6</accession>
    <name>A0A1U7EYT6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">UTP--glucose-1-phosphate uridylyltransferase AglF</fullName>
            <ecNumber evidence="4">2.7.7.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">aglF</name>
        <name evidence="4" type="synonym">graD3</name>
        <name evidence="4" type="ordered locus">NP_4674A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.9" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324042.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYT6"/>
    <dbReference type="STRING" id="348780.NP_4674A"/>
    <dbReference type="EnsemblBacteria" id="CAI50428">
        <property type="protein sequence ID" value="CAI50428"/>
        <property type="gene ID" value="NP_4674A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702772"/>
    <dbReference type="KEGG" id="nph:NP_4674A"/>
    <dbReference type="eggNOG" id="arCOG00664">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_2_0_2"/>
    <dbReference type="OrthoDB" id="15372at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003983">
        <property type="term" value="F:UTP:glucose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd04181">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053799">
        <property type="entry name" value="AglF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050065">
        <property type="entry name" value="GlmU-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041313">
        <property type="entry name" value="UDPGP_AglF_Halo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43584:SF8">
        <property type="entry name" value="N-ACETYLMURAMATE ALPHA-1-PHOSPHATE URIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43584">
        <property type="entry name" value="NUCLEOTIDYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="3">
        <location>
            <begin position="3"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="248" mass="27955" checksum="C171D5A7D8A476B7" modified="2017-05-10" version="1">MQAVVLAAGEGTRLRPLTEDKPKGMVEIDDKPILTHCFDQLVDLGADELVVVVGYMKEVIIDHYGDEYRDVPITYTHQRDQQGLAHALLTVEEHIDDDFMLMLGDNIFQANLEDVVRRQQEERADAAFLVEEVPWDEASRYGVCDTNQYGEITDVIEKPDDPPSNLVMTGFYTFTPAIFHACHLVQPSDRGEYEISEAIDLLLQSGRTIDAIGLEGWRIDVGYPEDRDEAERRLQNAAADETESVAVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ30</accession>
    <name>A0A1U7EZ30_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4850A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324128.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ30"/>
    <dbReference type="STRING" id="348780.NP_4850A"/>
    <dbReference type="EnsemblBacteria" id="CAI50516">
        <property type="protein sequence ID" value="CAI50516"/>
        <property type="gene ID" value="NP_4850A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702196"/>
    <dbReference type="KEGG" id="nph:NP_4850A"/>
    <dbReference type="eggNOG" id="arCOG11365">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1718268_0_0_2"/>
    <dbReference type="OrthoDB" id="206018at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="152" mass="15651" checksum="8E24265156353DD4" modified="2017-05-10" version="1">MDERRCTRRQVGAGVIGGLSVLVAGCLGNGDGNSSHGDGSYSVDIIDRDTDKAVADYHGHWHGDLPSVPVGEHISLGAVAEDDEGNEVTLGDDGLQLAAREVGDGGILSTDSHGNHVHINGDAAGETAVVVALHDGDHVEWESSAPIDVVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ36</accession>
    <name>A0A1U7EZ36_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">UPF0175 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4838A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50510.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939716.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ36"/>
    <dbReference type="STRING" id="348780.NP_4838A"/>
    <dbReference type="EnsemblBacteria" id="CAI50510">
        <property type="protein sequence ID" value="CAI50510"/>
        <property type="gene ID" value="NP_4838A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702190"/>
    <dbReference type="KEGG" id="nph:NP_4838A"/>
    <dbReference type="eggNOG" id="arCOG04629">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_175991_0_0_2"/>
    <dbReference type="OrthoDB" id="269969at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50510.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="96" mass="10699" checksum="E244F70FF0681837" modified="2017-05-10" version="1">MGTISARVPDELESELEAYLESETLDRSTAVRKLLSEGLEQWRREQALEALEAGEMTVSAAAEQAGLSVWELARLAEEHDITWVDSDHLEADLESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ47</accession>
    <name>A0A1U7EZ47_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">Protein translocase subunit SecY</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Protein transport protein SEC61 subunit alpha homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10 15" type="primary">secY</name>
        <name evidence="15" type="ordered locus">NP_4900A</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 11">The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the Sec protein translocase complex. Heterotrimer consisting of alpha (SecY), beta (SecG) and gamma (SecE) subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. May interact with SecDF, and other proteins may be involved.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10 13">Belongs to the SecY/SEC61-alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324153.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ47"/>
    <dbReference type="STRING" id="348780.NP_4900A"/>
    <dbReference type="EnsemblBacteria" id="CAI50541">
        <property type="protein sequence ID" value="CAI50541"/>
        <property type="gene ID" value="NP_4900A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703204"/>
    <dbReference type="KEGG" id="nph:NP_4900A"/>
    <dbReference type="eggNOG" id="arCOG04169">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031763_3_0_2"/>
    <dbReference type="OrthoDB" id="371914at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3370.10">
        <property type="entry name" value="SecY subunit domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01465">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026593">
        <property type="entry name" value="SecY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002208">
        <property type="entry name" value="SecY/SEC61-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030659">
        <property type="entry name" value="SecY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023201">
        <property type="entry name" value="SecY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019561">
        <property type="entry name" value="Translocon_Sec61/SecY_plug_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00967">
        <property type="entry name" value="3a0501s007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006341">
        <property type="entry name" value="PRK08568.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10906">
        <property type="entry name" value="SECY/SEC61-ALPHA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10559">
        <property type="entry name" value="Plug_translocon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00344">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004557">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00303">
        <property type="entry name" value="SECYTRNLCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103491">
        <property type="entry name" value="Preprotein translocase SecY subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00755">
        <property type="entry name" value="SECY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00756">
        <property type="entry name" value="SECY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10 12" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 10 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="72"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="117"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="147"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="182"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="245"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="282"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="366"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="427"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="Translocon Sec61/SecY plug" evidence="14">
        <location>
            <begin position="38"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF10559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAI50541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="491" mass="53387" checksum="A8570E3E71F81529" modified="2017-05-10" version="1">MSWKEAAEPVLTRLPAVKRPDRHVPFKRKLGWTAGVLVLYFFLTNVGIYGLGQGEDIFGQFRTILAGEQGSLLQVGIGPIVTASIVLQLLGGANLLGLNTDENPRDQALYQGLQKTLVIVMTALTAFPMVFAGFLQPSQAVADSLGISTGALGWIMFLQIFLGGMLILYMDEVISKWGVGSGIGLFIIAGVSQRLIGGFIAWSGLDAGYVGFFPRWYGILTGQVEMGPVLTQTGLFDLFLGPGELLALITTLLIFGIVVYAESVRVEIPLSHSRVKGARGRFPVKLIYASVLPMILVRALQANIQFLGRILNNQWAGMPAVIGEYSGSEQGFAEPTGGLFYYLAPIYSPEDWMWWLGETAAAPWQIMLRVGVDLTFMIVGGGIFAIFWVETTGMGPRATAEQIQRSGMQIPGFRQNPGTTEKVLERYIPQVTVIGGALVGLLAVMANMLGTIGQVTGTGLLLTVSITYKLYEEIAEEQLMEMHPMMREMFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ57</accession>
    <name>A0A1U7EZ57_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Small CPxCG-related zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4928A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324167.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ57"/>
    <dbReference type="STRING" id="348780.NP_4928A"/>
    <dbReference type="EnsemblBacteria" id="CAI50555">
        <property type="protein sequence ID" value="CAI50555"/>
        <property type="gene ID" value="NP_4928A"/>
    </dbReference>
    <dbReference type="GeneID" id="41345111"/>
    <dbReference type="KEGG" id="nph:NP_4928A"/>
    <dbReference type="eggNOG" id="arCOG09181">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_217069_0_0_2"/>
    <dbReference type="OrthoDB" id="280213at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055553">
        <property type="entry name" value="DUF7129"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033497">
        <property type="entry name" value="rubre_like_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="45" mass="4937" checksum="B131953D2DD83C6D" modified="2017-05-10" version="1">MRPISTASKADIYECFDCGVRIETTVAGRCDCGGEFVHLGRSRDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ71</accession>
    <name>A0A1U7EZ71_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Cob(I)alamin adenosyltransferase</fullName>
            <ecNumber evidence="1">2.5.1.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cobA</name>
        <name evidence="1" type="synonym">cobO</name>
        <name evidence="1" type="ordered locus">NP_4974A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.5.1.17" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324189.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ71"/>
    <dbReference type="STRING" id="348780.NP_4974A"/>
    <dbReference type="EnsemblBacteria" id="CAI50578">
        <property type="protein sequence ID" value="CAI50578"/>
        <property type="gene ID" value="NP_4974A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702620"/>
    <dbReference type="KEGG" id="nph:NP_4974A"/>
    <dbReference type="eggNOG" id="arCOG04678">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088595_2_0_2"/>
    <dbReference type="OrthoDB" id="24392at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008817">
        <property type="term" value="F:corrinoid adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003724">
        <property type="entry name" value="CblAdoTrfase_CobA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46638">
        <property type="entry name" value="CORRINOID ADENOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46638:SF1">
        <property type="entry name" value="CORRINOID ADENOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02572">
        <property type="entry name" value="CobA_CobO_BtuR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015617">
        <property type="entry name" value="Adensltrnsf_CobA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="221" mass="24065" checksum="7D228DC53F5C6621" modified="2017-05-10" version="1">MTDRPTPGRGRTPTAQAIDPAAPDEFGLTQVWWGDGKGKTTATLGMAFRAAGHGFRVHVLQFMKGGADSVADVRGEYNAITAVPGISYENLGHYGWHAMNDGTEERDHAAEAEAGLDRAHELVAAADDADLTSPFDPDSDPDDGVHMLVFDEILYAADRELVDPEAVVRLVEEKPENLELVLSGSHSRPEYLIESADLVTNVRKEKHPIDDGQRARMGTEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ94</accession>
    <name>A0A1U7EZ94_NATPD</name>
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        <submittedName>
            <fullName evidence="4">Formyltetrahydrofolate deformylase</fullName>
            <ecNumber evidence="4">3.5.1.10</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">purU</name>
        <name evidence="4" type="ordered locus">NP_5006A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.1.10" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50594.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ94"/>
    <dbReference type="STRING" id="348780.NP_5006A"/>
    <dbReference type="EnsemblBacteria" id="CAI50594">
        <property type="protein sequence ID" value="CAI50594"/>
        <property type="gene ID" value="NP_5006A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_5006A"/>
    <dbReference type="eggNOG" id="arCOG02826">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038395_3_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008864">
        <property type="term" value="F:formyltetrahydrofolate deformylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.170">
        <property type="entry name" value="Formyl transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004810">
        <property type="entry name" value="PurU"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004684">
        <property type="entry name" value="PRK06027.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42706">
        <property type="entry name" value="FORMYLTETRAHYDROFOLATE DEFORMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42706:SF1">
        <property type="entry name" value="FORMYLTETRAHYDROFOLATE DEFORMYLASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13740">
        <property type="entry name" value="ACT_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036480">
        <property type="entry name" value="FormyFH4_hydr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01575">
        <property type="entry name" value="FFH4HYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="Formyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="3">
        <location>
            <begin position="7"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50594.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="318" mass="35382" checksum="04952B3B8E825405" modified="2017-05-10" version="1">MTREYTEITVVGDDDTGLIARVTTLLFERDINIEDLDQAVREGIFRMTMSVDTAGMSCTESELRTALSELGDELGVDIQVRFPADRETRTIAVLVTKESHCLEALFEAWADGELGADISVIIGNHDDLEPLADHYGVDFYNIGDEKGTPDEERLLELLDDYDTDLIVLARYMRILSPNVVFRYEDRIINIHPSLLPAFPGAEAYRQAREEGVRIAGVTAHYVTTDLDQGPIITQRAFNAPAGASTEELERRGQPLEAEALLEAVQLHLDNATHVYHGRTELRDDVDTDDYQLGLPESFDEMVPDRPVDGLGEAVSGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZA7</accession>
    <name>A0A1U7EZA7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein eS31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rps31e</name>
        <name evidence="6" type="synonym">rps27ae</name>
        <name evidence="8" type="ordered locus">NP_5072A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the eukaryotic ribosomal protein eS31 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324237.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZA7"/>
    <dbReference type="STRING" id="348780.NP_5072A"/>
    <dbReference type="EnsemblBacteria" id="CAI50627">
        <property type="protein sequence ID" value="CAI50627"/>
        <property type="gene ID" value="NP_5072A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703177"/>
    <dbReference type="KEGG" id="nph:NP_5072A"/>
    <dbReference type="eggNOG" id="arCOG04183">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_179743_2_0_2"/>
    <dbReference type="OrthoDB" id="25142at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00777">
        <property type="entry name" value="Ribosomal_eS31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002906">
        <property type="entry name" value="Ribosomal_eS31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022845">
        <property type="entry name" value="Ribosomal_eS31_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001669">
        <property type="entry name" value="PRK00432.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01599">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01402">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Small ribosomal subunit protein eS31" evidence="7">
        <location>
            <begin position="3"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="6">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI50627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="44" mass="5209" checksum="EB15A00ED7F3DBD0" modified="2017-05-10" version="1">MARYEFYNEDGTTDREQCPRCGDTFLGEYGDRKHCGKCSYTEFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZD3</accession>
    <name>A0A1U7EZD3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcription regulator ThiR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">thiR</name>
        <name evidence="2" type="ordered locus">NP_5168A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324285.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZD3"/>
    <dbReference type="STRING" id="348780.NP_5168A"/>
    <dbReference type="EnsemblBacteria" id="CAI50675">
        <property type="protein sequence ID" value="CAI50675"/>
        <property type="gene ID" value="NP_5168A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702279"/>
    <dbReference type="KEGG" id="nph:NP_5168A"/>
    <dbReference type="eggNOG" id="arCOG00021">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054903_1_0_2"/>
    <dbReference type="OrthoDB" id="42697at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019293">
        <property type="entry name" value="ThiN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40730:SF5">
        <property type="entry name" value="HTH CRO_C1-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40730">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR PROTEIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10120">
        <property type="entry name" value="ThiN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="20"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="298" mass="31619" checksum="8EFF37B4A2CB9023" modified="2017-05-10" version="1">MKFIEELVVEEFLPTFRTELAGALRERGLTQSEVAGLLGISQSAVSKYAHGEVETNPDIAADDRFSELVDRLADGLASGEMSRVQALVETEVLIRQLERGDLLARLHEDAYPPLADYEGPLDIHDPDGELRMTERVRSSVRRGVRTLENTSGFAGLVPNVGSNLVEALPEAETIDDVAGVPGRILDIKGQATVPADPEFGVSGHVAKLLLTAREAGVDARAALNIRYDESIVAELEAAGYETAAFDAEADLDAAVQAAIAGSASVDVLYQTGGFGVEPVCYLLGSDASAVVAAVRELC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZG5</accession>
    <name>A0A1U7EZG5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Homolog to NAD-dependent epimerase/dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5218A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZG5"/>
    <dbReference type="STRING" id="348780.NP_5218A"/>
    <dbReference type="EnsemblBacteria" id="CAI50700">
        <property type="protein sequence ID" value="CAI50700"/>
        <property type="gene ID" value="NP_5218A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_5218A"/>
    <dbReference type="eggNOG" id="arCOG03095">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_11_4_2"/>
    <dbReference type="OrthoDB" id="312526at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05266">
        <property type="entry name" value="SDR_a4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051783">
        <property type="entry name" value="NAD(P)-dependent_oxidoreduct"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48079:SF6">
        <property type="entry name" value="NAD(P)-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48079">
        <property type="entry name" value="PROTEIN YEEZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="1">
        <location>
            <begin position="10"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="297" mass="32474" checksum="80575CEE048A58D7" modified="2017-05-10" version="1">MTMRVGILGCGYVGLELARQLRDTHDVVGMRRSEAGLEAVEAAGAKPIQGDLTDPESLSALPSLDAVVFAASSGGRGADAAERVYVDGLRTVIETLGERSDPPERLVYTSSTGVYGDHDGDWVDESTPLSPTTDKTQVLAEAERLARERTADYGISGGVARFAGLYGPDRYRLNRYLDGPVTDGYLNMIHRDDAAGVVRFMLTTATESEVLVAVDDEPVDKWAFADWLADECGVESPPKRTKEERLEDDSLSEAARRRILTSKRCSNDRVRELGYSFSYPTYREGYQAAVDAYRAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZK2</accession>
    <name>A0A1U7EZK2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF8001 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5278A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324339.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZK2"/>
    <dbReference type="STRING" id="348780.NP_5278A"/>
    <dbReference type="EnsemblBacteria" id="CAI50730">
        <property type="protein sequence ID" value="CAI50730"/>
        <property type="gene ID" value="NP_5278A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702317"/>
    <dbReference type="KEGG" id="nph:NP_5278A"/>
    <dbReference type="eggNOG" id="arCOG08136">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_175997_0_0_2"/>
    <dbReference type="OrthoDB" id="258836at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058314">
        <property type="entry name" value="DUF8001"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26008">
        <property type="entry name" value="DUF8001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8001" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="79" mass="8971" checksum="BF5379547EC39198" modified="2017-05-10" version="1">MSGTTVIEPGECSIEELLARLRDGERFVVRTEILGSTEELTLRFDGETYYCDSPTILHKHTDEAEMRTCIEDLGFADEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZK4</accession>
    <name>A0A1U7EZK4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">tRNA 2-thiolation protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">tuc1</name>
        <name evidence="6" type="ordered locus">NP_5322A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324361.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZK4"/>
    <dbReference type="STRING" id="348780.NP_5322A"/>
    <dbReference type="EnsemblBacteria" id="CAI50752">
        <property type="protein sequence ID" value="CAI50752"/>
        <property type="gene ID" value="NP_5322A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702332"/>
    <dbReference type="KEGG" id="nph:NP_5322A"/>
    <dbReference type="eggNOG" id="arCOG00042">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026481_1_1_2"/>
    <dbReference type="OrthoDB" id="33422at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002144">
        <property type="term" value="C:cytosolic tRNA wobble base thiouridylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01713">
        <property type="entry name" value="CTU1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056369">
        <property type="entry name" value="CTU1-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000541">
        <property type="entry name" value="Ncs6/Tuc1/Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035107">
        <property type="entry name" value="tRNA_thiolation_TtcA_Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054306">
        <property type="entry name" value="TtuA-like_LIM_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00269">
        <property type="entry name" value="TIGR00269 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF047749">
        <property type="entry name" value="tRNAThiolNcsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11807">
        <property type="entry name" value="ATPASES OF THE PP SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11807:SF12">
        <property type="entry name" value="CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22082">
        <property type="entry name" value="TtuA_LIM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004976">
        <property type="entry name" value="ATPase_YdaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="2-thiouridine synthetase TtuA-like N-terminal LIM" evidence="5">
        <location>
            <begin position="2"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA(Ile)-lysidine/2-thiocytidine synthase N-terminal" evidence="4">
        <location>
            <begin position="56"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="3"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="6"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="59"/>
            <end position="61"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR004976-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR004976-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF22082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI50752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="325" mass="35678" checksum="5102B62672496703" modified="2017-05-10" version="1">MQCDKCGGEAVMHAAYSGMHLCAEHFCRSVDSRVRKRIRDDALLPESASPDDPQTWLIGLSGGKDSVVLTHILDETFAADPRVELVALTIHEGIEGYRDASLEACRELTTDLDIEHAVVNYADEYGVEMDNVAEDDPLGMAPCAYCGVFRRDALSAYADEYGADKLLTGHNLDDEAQTAMMNLLSGDIKQVAKHFDASLGSFEDRAVDGDPFVPRAKPLRDIPEKEVALYAHLKELPSHMAECPHASEAYRGEIQDHLHELEDEHPGTRHSIMSGYEELARLAAEAYRDDGDGPRGECDRCGAPTDNEVCRKCELVDAVDGVADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZL4</accession>
    <name>A0A1U7EZL4_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Probable cobalamin biosynthesis protein CobD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">cbiB</name>
        <name evidence="11" type="synonym">cobD</name>
        <name evidence="12" type="ordered locus">NP_5308A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cell membrane</location>
            <topology evidence="2 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the CobD/CbiB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324354.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZL4"/>
    <dbReference type="STRING" id="348780.NP_5308A"/>
    <dbReference type="EnsemblBacteria" id="CAI50745">
        <property type="protein sequence ID" value="CAI50745"/>
        <property type="gene ID" value="NP_5308A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702567"/>
    <dbReference type="KEGG" id="nph:NP_5308A"/>
    <dbReference type="eggNOG" id="arCOG04274">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054212_0_2_2"/>
    <dbReference type="OrthoDB" id="46105at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015420">
        <property type="term" value="F:ABC-type vitamin B12 transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048472">
        <property type="term" value="F:threonine-phosphate decarboxylase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00024">
        <property type="entry name" value="CobD_CbiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004485">
        <property type="entry name" value="Cobalamin_biosynth_CobD/CbiB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34308">
        <property type="entry name" value="COBALAMIN BIOSYNTHESIS PROTEIN CBIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34308:SF1">
        <property type="entry name" value="COBALAMIN BIOSYNTHESIS PROTEIN CBIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03186">
        <property type="entry name" value="CobD_Cbib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="12" id="KW-0436">Ligase</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="287"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI50745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="304" mass="30300" checksum="66BE2B11159499F9" modified="2017-05-10" version="1">MNGSGLLLAVAVAAALEALVGEPPARIHPVALLGRAVAPLGERSFSWPALAGTVAAVGVPAAAAGVAYLTVAAAAALGAAAAAVTAGLVLWTTTSLRLLLSSAADVIEASDNDPAAAKAALPALAGRDAASLSPPLLRSAAVESLAENLSDGLVGPLSAFVVLSFVSLPAAAAAAAAFKAVNTMDSMFGYPGRFGWASARLDDLMMFVPARLSAALLGPAAADPDAPVRARRYADKPASPNAGWPMGTLAAALNVRLEKPDSYRLNELASLPAAADGQRALSATRRAGIAAYVIAALVGVVQWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZN5</accession>
    <name>A0A1U7EZN5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_5350A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZN5"/>
    <dbReference type="EnsemblBacteria" id="CAI50766">
        <property type="protein sequence ID" value="CAI50766"/>
        <property type="gene ID" value="NP_5350A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_5350A"/>
    <dbReference type="HOGENOM" id="CLU_3362600_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="35" mass="3538" checksum="402CE8155EBA06B6" modified="2017-05-10" version="1">MQLLDRHLRAEEVALYLAGAATALAGAALASGVTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZP3</accession>
    <name>A0A1U7EZP3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_5367B</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CCI69589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZP3"/>
    <dbReference type="STRING" id="348780.NP_5367B"/>
    <dbReference type="EnsemblBacteria" id="CCI69589">
        <property type="protein sequence ID" value="CCI69589"/>
        <property type="gene ID" value="NP_5367B"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_5367B"/>
    <dbReference type="HOGENOM" id="CLU_3075450_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCI69589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="52" mass="5896" checksum="47C8338CAA461563" modified="2017-05-10" version="1">MPPGQPVHRCDGRISVVCQCCGRRFKSPIFDGEHDNYCSDGCRRRAEWGVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZR6</accession>
    <name>A0A1U7EZR6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5227G</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CCI69587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_169303789.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZR6"/>
    <dbReference type="STRING" id="348780.NP_5227G"/>
    <dbReference type="EnsemblBacteria" id="CCI69587">
        <property type="protein sequence ID" value="CCI69587"/>
        <property type="gene ID" value="NP_5227G"/>
    </dbReference>
    <dbReference type="GeneID" id="54763331"/>
    <dbReference type="KEGG" id="nph:NP_5227G"/>
    <dbReference type="eggNOG" id="arCOG14540">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3194603_0_0_2"/>
    <dbReference type="OrthoDB" id="226402at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCI69587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="45" mass="5068" checksum="35659175C9FA08BF" modified="2017-05-10" version="1">MTNDEIEPLVPEMPRQSSTDPVRADGGRRPRYDNAEVDAPLVPQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILS3</accession>
    <name>Q3ILS3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3240A</name>
        <name evidence="2" type="ordered locus">NP_7064A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>PL23</name>
    </geneLocation>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
            <strain evidence="2">Gabara</strain>
            <plasmid evidence="2">PL23</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CR936259">
        <property type="protein sequence ID" value="CAI50948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323333.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILS3"/>
    <dbReference type="STRING" id="348780.NP_3240A"/>
    <dbReference type="EnsemblBacteria" id="CAI49711">
        <property type="protein sequence ID" value="CAI49711"/>
        <property type="gene ID" value="NP_3240A"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI50948">
        <property type="protein sequence ID" value="CAI50948"/>
        <property type="gene ID" value="NP_7064A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703491"/>
    <dbReference type="KEGG" id="nph:NP_3240A"/>
    <dbReference type="KEGG" id="nph:NP_7064A"/>
    <dbReference type="HOGENOM" id="CLU_2784216_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="68" mass="7443" checksum="A07BB851962500E9" modified="2005-11-08" version="1">MASNRTVETHIHYTVDSEGRPQRAYHSISEARAAVESDDYWHSNEVDYVPDVPLIEAGSDSKLPGGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILS4</accession>
    <name>Q3ILS4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">XerC/D-like integrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">NP_3238A</name>
        <name evidence="9" type="ordered locus">NP_7062A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9 10">
        <name>PL23</name>
    </geneLocation>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
            <strain evidence="9">Gabara</strain>
            <plasmid evidence="9">PL23</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CR936259">
        <property type="protein sequence ID" value="CAI50947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323332.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILS4"/>
    <dbReference type="STRING" id="348780.NP_3238A"/>
    <dbReference type="EnsemblBacteria" id="CAI49710">
        <property type="protein sequence ID" value="CAI49710"/>
        <property type="gene ID" value="NP_3238A"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI50947">
        <property type="protein sequence ID" value="CAI50947"/>
        <property type="gene ID" value="NP_7062A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703490"/>
    <dbReference type="KEGG" id="nph:NP_3238A"/>
    <dbReference type="KEGG" id="nph:NP_7062A"/>
    <dbReference type="eggNOG" id="arCOG01250">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045500_0_0_2"/>
    <dbReference type="OrthoDB" id="198497at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00397">
        <property type="entry name" value="DNA_BRE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004107">
        <property type="entry name" value="Integrase_SAM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050090">
        <property type="entry name" value="Tyrosine_recombinase_XerCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30349:SF41">
        <property type="entry name" value="INTEGRASE_RECOMBINASE PROTEIN MJ0367-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30349">
        <property type="entry name" value="PHAGE INTEGRASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02899">
        <property type="entry name" value="Phage_int_SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core-binding (CB)" evidence="7">
        <location>
            <begin position="10"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="6">
        <location>
            <begin position="117"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="301"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="301"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="320"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI50947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="354" mass="41251" checksum="14322B4FFAE66EAB" modified="2005-11-08" version="1">MTRRSDPEALSVSDAVKRFVRRMRRDKAESTVRTYRLDLRQFVQWCDAEGIEQVSELEKIDFELYQDDRAAEIEPTSLENQMGLLKRFVKFCEDIGALPEGFHESVHVPRATVEEQSRDEKLSTEDATELLLFFRDKSNDLFGSKWHAILEVIWHTGARIGGIRALDLEDFDSEFHVLEFRHRPRQGTPLKNKQDGERDVAILPVVAEALECYIEEHRIEQHDEEGRAPLFTTRSQQARPSTNTVRAWVYQATQPCWHTDEPCPHDKRREFCDWTSSAASSKCPSSRSPHAVRTGAITFHRDRGFDPNDTSERVNASLRTIEKHYDKAARREKMENRRRPQLEKLSLNNESERE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILS5</accession>
    <name>Q3ILS5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3334A</name>
        <name evidence="2" type="ordered locus">NP_7058A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>PL23</name>
    </geneLocation>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
            <strain evidence="2">Gabara</strain>
            <plasmid evidence="2">PL23</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CR936259">
        <property type="protein sequence ID" value="CAI50945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323379.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILS5"/>
    <dbReference type="STRING" id="348780.NP_3334A"/>
    <dbReference type="EnsemblBacteria" id="CAI49758">
        <property type="protein sequence ID" value="CAI49758"/>
        <property type="gene ID" value="NP_3334A"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI50945">
        <property type="protein sequence ID" value="CAI50945"/>
        <property type="gene ID" value="NP_7058A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703489"/>
    <dbReference type="KEGG" id="nph:NP_3334A"/>
    <dbReference type="KEGG" id="nph:NP_7058A"/>
    <dbReference type="HOGENOM" id="CLU_1623508_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="163" mass="18529" checksum="45FF8108B3E201D1" modified="2005-11-08" version="1">MSLSRTPGPVDHEADCEYPCCDEDAVACVPDPGTIAGKRFCDFHLALWADCDRGDWDILDQLDVRHLAADDSFLKLDDAPPELERKVRYERIGVDHRGLAHYIVPGRRDDEAETIIVVDADLELVDSGTIPAHATLEDWVDHIKTDRRWVEVDDRFHPRRGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILU7</accession>
    <name>Q3ILU7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3290A</name>
        <name evidence="3" type="ordered locus">NP_7014A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>PL23</name>
    </geneLocation>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
            <strain evidence="3">Gabara</strain>
            <plasmid evidence="3">PL23</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CR936259">
        <property type="protein sequence ID" value="CAI50923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323357.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILU7"/>
    <dbReference type="STRING" id="348780.NP_3290A"/>
    <dbReference type="EnsemblBacteria" id="CAI49736">
        <property type="protein sequence ID" value="CAI49736"/>
        <property type="gene ID" value="NP_3290A"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI50923">
        <property type="protein sequence ID" value="CAI50923"/>
        <property type="gene ID" value="NP_7014A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703467"/>
    <dbReference type="KEGG" id="nph:NP_3290A"/>
    <dbReference type="KEGG" id="nph:NP_7014A"/>
    <dbReference type="HOGENOM" id="CLU_2217130_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="106" mass="10564" checksum="60BB210EA9070656" modified="2005-11-08" version="1">MAGQSQSYVSKGAGAVEGVSNRTGLSEARIMGTVRVIGGAMIGIAILALVLTEVFTLEAMDIQEGPFSGVVDSLESTGGAALGLLVIGLLVAAANRVMGFFGSGGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILV1</accession>
    <name>Q3ILV1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Type II toxin-antitoxin system HicB family antitoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3282A</name>
        <name evidence="3" type="ordered locus">NP_7006A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>PL23</name>
    </geneLocation>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
            <strain evidence="3">Gabara</strain>
            <plasmid evidence="3">PL23</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CR936259">
        <property type="protein sequence ID" value="CAI50919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323353.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILV1"/>
    <dbReference type="STRING" id="348780.NP_3282A"/>
    <dbReference type="EnsemblBacteria" id="CAI49732">
        <property type="protein sequence ID" value="CAI49732"/>
        <property type="gene ID" value="NP_3282A"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI50919">
        <property type="protein sequence ID" value="CAI50919"/>
        <property type="gene ID" value="NP_7006A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703463"/>
    <dbReference type="KEGG" id="nph:NP_3282A"/>
    <dbReference type="KEGG" id="nph:NP_7006A"/>
    <dbReference type="eggNOG" id="arCOG02413">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_181226_0_0_2"/>
    <dbReference type="OrthoDB" id="201961at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055811">
        <property type="entry name" value="DUF7387"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035069">
        <property type="entry name" value="TTHA1013/TTHA0281-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24113">
        <property type="entry name" value="DUF7387"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143100">
        <property type="entry name" value="TTHA1013/TTHA0281-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="84" mass="9025" checksum="50ED7D164648B01C" modified="2005-11-08" version="1">MATVSEPPAGVEFVHEDDGRVTARHVESGVASFGDTEAEALRELADALDSHFGDGERIDDPEAYLEDQGIDVEIGENGSPPWLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILX6</accession>
    <name>Q3ILX6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TRAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_6228A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324491.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILX6"/>
    <dbReference type="EnsemblBacteria" id="CAI50894">
        <property type="protein sequence ID" value="CAI50894"/>
        <property type="gene ID" value="NP_6228A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694645"/>
    <dbReference type="KEGG" id="nph:NP_6228A"/>
    <dbReference type="HOGENOM" id="CLU_121271_0_0_2"/>
    <dbReference type="OrthoDB" id="28569at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TRAM" evidence="2">
        <location>
            <begin position="78"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="139" mass="14909" checksum="88B4BC22E47FCA63" modified="2005-11-08" version="1">MTEIPDPLRTLYSASIEKQDGTYTIEVPLQEIEHGAVEPGETYRIAILESTETDTSSQQPSRPAPAPTADADEPTGPPVDEGEIREVTVETVGDQGDGIAKVERGYVIIVPDAAPGDEPTVEIQQVRENVAFADVVGHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILY1</accession>
    <name>Q3ILY1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">N-methylhydantoinase (ATP-hydrolyzing) B</fullName>
            <ecNumber evidence="3">3.5.2.14</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">hyuB2</name>
        <name evidence="3" type="ordered locus">NP_6216A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.2.14" evidence="3"/>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324486.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILY1"/>
    <dbReference type="EnsemblBacteria" id="CAI50888">
        <property type="protein sequence ID" value="CAI50888"/>
        <property type="gene ID" value="NP_6216A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694595"/>
    <dbReference type="KEGG" id="nph:NP_6216A"/>
    <dbReference type="HOGENOM" id="CLU_020413_1_0_2"/>
    <dbReference type="OrthoDB" id="8261at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017168">
        <property type="term" value="F:5-oxoprolinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047423">
        <property type="term" value="F:N-methylhydantoinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003692">
        <property type="entry name" value="Hydantoinase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="5-OXOPROLINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365:SF23">
        <property type="entry name" value="HYPOTHETICAL 5-OXOPROLINASE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02538">
        <property type="entry name" value="Hydantoinase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydantoinase B/oxoprolinase" evidence="2">
        <location>
            <begin position="15"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="566"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="574"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="618" mass="68208" checksum="CA2896EB4A35D90F" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM41</accession>
    <name>Q3IM41_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">HTH domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_6088A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324426.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM41"/>
    <dbReference type="EnsemblBacteria" id="CAI50824">
        <property type="protein sequence ID" value="CAI50824"/>
        <property type="gene ID" value="NP_6088A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694612"/>
    <dbReference type="KEGG" id="nph:NP_6088A"/>
    <dbReference type="HOGENOM" id="CLU_142662_0_0_2"/>
    <dbReference type="OrthoDB" id="195563at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="2">
        <location>
            <begin position="25"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="122" mass="13350" checksum="557AEDD0464C6BE3" modified="2005-11-08" version="1">MPVDFETHEPGNPRVDLSEGTNARRLLEFLLKTPSVGYTPAELAEETGVPRGSVGPTLNRLEAAGLVRHKEPYWAAAEDDRIAAATAAFIGVETVASTYSDDWYAQNDGWDEELPDLSDEET</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM63</accession>
    <name>Q3IM63_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">3-oxoacid CoA-transferase alpha subunit</fullName>
            <ecNumber evidence="2">2.8.3.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_6034A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.8.3.-" evidence="2"/>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324404.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM63"/>
    <dbReference type="EnsemblBacteria" id="CAI50797">
        <property type="protein sequence ID" value="CAI50797"/>
        <property type="gene ID" value="NP_6034A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694591"/>
    <dbReference type="KEGG" id="nph:NP_6034A"/>
    <dbReference type="HOGENOM" id="CLU_049557_0_0_2"/>
    <dbReference type="OrthoDB" id="301771at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008410">
        <property type="term" value="F:CoA-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1080.10">
        <property type="entry name" value="Glutaconate Coenzyme A-transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004165">
        <property type="entry name" value="CoA_trans_fam_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13707:SF60">
        <property type="entry name" value="ACETATE COA-TRANSFERASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13707">
        <property type="entry name" value="KETOACID-COENZYME A TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01144">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00882">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="340" mass="37612" checksum="1D1DC219644E7417" modified="2005-11-08" version="1">MNVIDEGEGELVGWEHPDEMRAWNQEHRSRAFEDKRTTAAEAVDQFVDDGDLLASGGFGHVRIATPILHEIIRQEVTDLTLSGKTTVFDADLLIAAGAVSKVETAYCFAHETRGLAPAGRRRVEEGDVEVVAEASNATLQWRFLAAKMGVPFIPTRVLAGTDTFEKSSAKIVEDPWTGEPITLVPACYPDVVCIHVTKADKYGNAVIDGISVEDPELAGAAKRLILTAEEIVDSDELRSRSEDVEIPYFLVDAVVEAPYGSHPGEMPYQYYFDEDHLEEWMELTETESGIDEYLDRYVTGTDDFEEYLEAIGGETRLAELEAIEKYESEEASSHDGGDRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM67</accession>
    <name>Q3IM67_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_6024A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM67"/>
    <dbReference type="EnsemblBacteria" id="CAI50792">
        <property type="protein sequence ID" value="CAI50792"/>
        <property type="gene ID" value="NP_6024A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_6024A"/>
    <dbReference type="HOGENOM" id="CLU_220700_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="34" mass="3677" checksum="3FA9ABECA1A74153" modified="2005-11-08" version="1">MSLDDAVEEALATYGMSRSEEAEETGRTVATLQD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMI4</accession>
    <name>DCD_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">dCTP deaminase</fullName>
            <ecNumber evidence="1">3.5.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Deoxycytidine triphosphate deaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dcd</name>
        <name type="ordered locus">NP_5166A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the deamination of dCTP to dUTP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>dCTP + H2O + H(+) = dUTP + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:22680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
            <dbReference type="ChEBI" id="CHEBI:61555"/>
            <dbReference type="EC" id="3.5.4.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the dCTP deaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.13" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324284.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMI4"/>
    <dbReference type="SMR" id="Q3IMI4"/>
    <dbReference type="STRING" id="348780.NP_5166A"/>
    <dbReference type="EnsemblBacteria" id="CAI50674">
        <property type="protein sequence ID" value="CAI50674"/>
        <property type="gene ID" value="NP_5166A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702659"/>
    <dbReference type="KEGG" id="nph:NP_5166A"/>
    <dbReference type="eggNOG" id="arCOG04048">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087476_2_1_2"/>
    <dbReference type="OrthoDB" id="33242at2157"/>
    <dbReference type="UniPathway" id="UPA00610">
        <property type="reaction ID" value="UER00665"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008829">
        <property type="term" value="F:dCTP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006226">
        <property type="term" value="P:dUMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006229">
        <property type="term" value="P:dUTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015949">
        <property type="term" value="P:nucleobase-containing small molecule interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.40.10:FF:000005">
        <property type="entry name" value="dCTP deaminase, dUMP-forming"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00146">
        <property type="entry name" value="dCTP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011962">
        <property type="entry name" value="dCTP_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02274">
        <property type="entry name" value="dCTP_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42680">
        <property type="entry name" value="DCTP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42680:SF3">
        <property type="entry name" value="DCTP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22769">
        <property type="entry name" value="DCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="dUTPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0546">Nucleotide metabolism</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000009766" description="dCTP deaminase">
        <location>
            <begin position="1"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="159"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="160"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="174"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="186"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="105"/>
            <end position="110"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="131"/>
            <end position="133"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="195" mass="21446" checksum="D33EC4A1AEFCDA7A" modified="2005-11-08" version="1">MILSDADIRRRLDEGDLAIEPLDDPELQVQPASVDLRLGREFLEFQRANISCIHPTSEREVDEYVDETYVEEGEEFVLHPGDFVLGTTKERVEIPPDLIAHVEGRSSLGRLAIVVHATAGLCDPGYQGQITLELSNLGTAPVALSPGMRISQLTFTELKSPAERPYGAERGSKYQGQTGPQASRIQGDREFGGDQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-12-06" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMW6</accession>
    <name>RL15_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL15</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">50S ribosomal protein L15</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl15</name>
        <name type="ordered locus">NP_4898A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMW6"/>
    <dbReference type="SMR" id="Q3IMW6"/>
    <dbReference type="STRING" id="348780.NP_4898A"/>
    <dbReference type="EnsemblBacteria" id="CAI50540">
        <property type="protein sequence ID" value="CAI50540"/>
        <property type="gene ID" value="NP_4898A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_4898A"/>
    <dbReference type="eggNOG" id="arCOG00779">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109163_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.990.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01341">
        <property type="entry name" value="Ribosomal_uL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027386">
        <property type="entry name" value="Rbsml_uL15_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030878">
        <property type="entry name" value="Ribosomal_uL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11721">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11721:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000104868" description="Large ribosomal subunit protein uL15">
        <location>
            <begin position="1"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="135"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="33"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="142"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="163" mass="17918" checksum="35A75C50D5C8C09C" modified="2005-11-08" version="1">MSKKRRQRGSRTHGGGSHKNRRGAGHRGGRGNAGRDKHEFHNHEPLGKSGFKRPQKTRRDVETVNLRELDEDIAVLVEDGIAEESDDGYVVDARDVVEDGYEADVVKVLGAGTVYNELEIVADAFSESAEQALEVADGEAVLSERGEELEAEKDSTDEEDEES</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-11-28" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMX2</accession>
    <name>RL6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl6</name>
        <name type="ordered locus">NP_4886A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324146.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMX2"/>
    <dbReference type="SMR" id="Q3IMX2"/>
    <dbReference type="STRING" id="348780.NP_4886A"/>
    <dbReference type="EnsemblBacteria" id="CAI50534">
        <property type="protein sequence ID" value="CAI50534"/>
        <property type="gene ID" value="NP_4886A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703147"/>
    <dbReference type="KEGG" id="nph:NP_4886A"/>
    <dbReference type="eggNOG" id="arCOG04090">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065464_0_0_2"/>
    <dbReference type="OrthoDB" id="7144at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.930.12:FF:000008">
        <property type="entry name" value="50S ribosomal protein L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.930.12">
        <property type="entry name" value="Ribosomal protein L6, alpha-beta domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01365_A">
        <property type="entry name" value="Ribosomal_uL6_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000702">
        <property type="entry name" value="Ribosomal_uL6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036789">
        <property type="entry name" value="Ribosomal_uL6-like_a/b-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020040">
        <property type="entry name" value="Ribosomal_uL6_a/b-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019907">
        <property type="entry name" value="Ribosomal_uL6_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004037">
        <property type="entry name" value="PRK05518.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03653">
        <property type="entry name" value="uL6_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655:SF16">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655">
        <property type="entry name" value="60S/50S RIBOSOMAL PROTEIN L6/L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00347">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002162">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56053">
        <property type="entry name" value="Ribosomal protein L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000260993" description="Large ribosomal subunit protein uL6">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="177" mass="19587" checksum="C99E68E8A1ED9F45" modified="2005-11-08" version="1">MRTEIEIPDEVTATMDHLEVTVEGPNGSVTRRLWYPDITVDVEDGAVVIETDDDNAKTRSTVGTFESHVTNMFHGVTEGWEYSMEVFYSHFPMQVSVEGGDIVIENFLGEKAPRKTPVRGDTSVEVDGEELTVSGPSIEDVGQTAADIEQLTRVSDKDTRVFQDGVYITETPDRGDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q3INT0</accession>
    <name>Q3INT0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Smc-like protein Sph2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">sph2</name>
        <name evidence="6" type="ordered locus">NP_4262A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the Sph1/Sph2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323838.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3INT0"/>
    <dbReference type="STRING" id="348780.NP_4262A"/>
    <dbReference type="EnsemblBacteria" id="CAI50222">
        <property type="protein sequence ID" value="CAI50222"/>
        <property type="gene ID" value="NP_4262A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702014"/>
    <dbReference type="KEGG" id="nph:NP_4262A"/>
    <dbReference type="eggNOG" id="arCOG00373">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_409187_0_0_2"/>
    <dbReference type="OrthoDB" id="241568at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045487">
        <property type="entry name" value="ASRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32114">
        <property type="entry name" value="ABC TRANSPORTER ABCH.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32114:SF2">
        <property type="entry name" value="ABC TRANSPORTER ABCH.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad50/SbcC-type AAA" evidence="5">
        <location>
            <begin position="12"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="351"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="236"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00049666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI50222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="644" mass="73364" checksum="14D69FFE030985F7" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-02-06" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ISC1</accession>
    <name>PHNC1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphonates import ATP-binding protein PhnC 1</fullName>
            <ecNumber evidence="1">7.3.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">phnC1</name>
        <name type="synonym">abc17a</name>
        <name type="ordered locus">NP_1750A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the ABC transporter complex PhnCDE involved in phosphonates import. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>phosphonate(out) + ATP + H2O = phosphonate(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16215"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.3.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The complex is composed of two ATP-binding proteins (PhnC), two transmembrane proteins (PhnE) and a solute-binding protein (PhnD).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. Phosphonates importer (TC 3.A.1.9.1) family.</text>
    </comment>
    <dbReference type="EC" id="7.3.2.2" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322599.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ISC1"/>
    <dbReference type="SMR" id="Q3ISC1"/>
    <dbReference type="STRING" id="348780.NP_1750A"/>
    <dbReference type="EnsemblBacteria" id="CAI48966">
        <property type="protein sequence ID" value="CAI48966"/>
        <property type="gene ID" value="NP_1750A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702420"/>
    <dbReference type="KEGG" id="nph:NP_1750A"/>
    <dbReference type="eggNOG" id="arCOG00206">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="OrthoDB" id="302885at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015416">
        <property type="term" value="F:ABC-type phosphonate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050086">
        <property type="entry name" value="MetN_ABC_transporter-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43166">
        <property type="entry name" value="AMINO ACID IMPORT ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43166:SF6">
        <property type="entry name" value="PHOSPHONATES IMPORT ATP-BINDING PROTEIN PHNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51249">
        <property type="entry name" value="PHNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0918">Phosphonate transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000274773" description="Phosphonates import ATP-binding protein PhnC 1">
        <location>
            <begin position="1"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="1">
        <location>
            <begin position="2"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01713"/>
        </source>
    </evidence>
    <sequence length="233" mass="24796" checksum="3CC354CCEA3D9A86" modified="2005-11-08" version="1">MLSVSGLTKRYGDERALDDVSVDLQRGELTVLVGRSGAGKTTLLRCLDGLEQPDDGTIELDGRAPATTDVALVFQAGALVDSKSALSNVLDGALGRLPSWRELLGVYPADEKRAAIARLHDVGLGGYADRRVGTLSGGQRQRVGIARALQQEPAVLLADEPVASLDPETGRDVLERVAAVVRDDDLIGVVSLHQPRLAEPIADRYLGLADGRLVLDRPAAALDREDITAVYDD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-21" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ISP9</accession>
    <name>DAPB_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4-hydroxy-tetrahydrodipicolinate reductase</fullName>
            <shortName evidence="1">HTPA reductase</shortName>
            <ecNumber evidence="1">1.17.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dapB</name>
        <name type="ordered locus">NP_1492A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-2,3,4,5-tetrahydrodipicolinate + NAD(+) + H2O = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:35323"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="EC" id="1.17.1.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-2,3,4,5-tetrahydrodipicolinate + NADP(+) + H2O = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:35331"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="EC" id="1.17.1.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 4/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DapB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Was originally thought to be a dihydrodipicolinate reductase (DHDPR), catalyzing the conversion of dihydrodipicolinate to tetrahydrodipicolinate. However, it was shown in E.coli that the substrate of the enzymatic reaction is not dihydrodipicolinate (DHDP) but in fact (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid (HTPA), the product released by the DapA-catalyzed reaction.</text>
    </comment>
    <dbReference type="EC" id="1.17.1.8" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322471.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ISP9"/>
    <dbReference type="SMR" id="Q3ISP9"/>
    <dbReference type="STRING" id="348780.NP_1492A"/>
    <dbReference type="EnsemblBacteria" id="CAI48837">
        <property type="protein sequence ID" value="CAI48837"/>
        <property type="gene ID" value="NP_1492A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702657"/>
    <dbReference type="KEGG" id="nph:NP_1492A"/>
    <dbReference type="eggNOG" id="arCOG04393">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047479_2_2_2"/>
    <dbReference type="OrthoDB" id="195035at2157"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00018"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008839">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate reductase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016726">
        <property type="term" value="F:oxidoreductase activity, acting on CH or CH2 groups, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02274">
        <property type="entry name" value="DHDPR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00102">
        <property type="entry name" value="DapB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022663">
        <property type="entry name" value="DapB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000846">
        <property type="entry name" value="DapB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022664">
        <property type="entry name" value="DapB_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023940">
        <property type="entry name" value="DHDPR_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00036">
        <property type="entry name" value="dapB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20836:SF0">
        <property type="entry name" value="4-HYDROXY-TETRAHYDRODIPICOLINATE REDUCTASE 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20836">
        <property type="entry name" value="DIHYDRODIPICOLINATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05173">
        <property type="entry name" value="DapB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01113">
        <property type="entry name" value="DapB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000161">
        <property type="entry name" value="DHPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01298">
        <property type="entry name" value="DAPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword id="KW-0457">Lysine biosynthesis</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000228408" description="4-hydroxy-tetrahydrodipicolinate reductase">
        <location>
            <begin position="1"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="140"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="144"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="84"/>
            <end position="86"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="108"/>
            <end position="111"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>(S)-2,3,4,5-tetrahydrodipicolinate</name>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="150"/>
            <end position="151"/>
        </location>
        <ligand>
            <name>(S)-2,3,4,5-tetrahydrodipicolinate</name>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="246" mass="26301" checksum="AA1E41CDC11BAEC3" modified="2005-11-08" version="1">MNVVVVGATGRTGSEIVAEASERGHDVSGVARSQTSVGDVRVYPTEEFPELLDNADAVIDFTVPEATREHVQAAAAAGVPYVIGTTGFDDDGMAALRTASESTAVLKASNFARGVQALLRVVEAGVEALPEYDIELTETHHNGKRDAPSGTANTILDVVDETRDEALDRTHGREGEHRRGDDEVGVHVRRAGTVRGEHELLLAGNDEVLSLTHRAESRRVFAAGAVDAAEWLADRSAGWYDFEDTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ISW4</accession>
    <name>Q3ISW4_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase</fullName>
            <ecNumber evidence="4">2.5.1.114</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">tRNA wyosine derivatives biosynthesis protein Taw2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4 6" type="primary">taw2</name>
        <name evidence="6" type="ordered locus">NP_1360A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">S-adenosyl-L-methionine-dependent transferase that acts as a component of the wyosine derivatives biosynthesis pathway. Catalyzes the transfer of the alpha-amino-alpha-carboxypropyl (acp) group from S-adenosyl-L-methionine to 4-demethylwyosine (imG-14), forming 7-aminocarboxypropyl-demethylwyosine (wybutosine-86) at position 37 of tRNA(Phe).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>4-demethylwyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine = 4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36355"/>
            <dbReference type="Rhea" id="RHEA-COMP:10164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10378"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64315"/>
            <dbReference type="ChEBI" id="CHEBI:73550"/>
            <dbReference type="EC" id="2.5.1.114"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.114" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322406.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ISW4"/>
    <dbReference type="STRING" id="348780.NP_1360A"/>
    <dbReference type="EnsemblBacteria" id="CAI48771">
        <property type="protein sequence ID" value="CAI48771"/>
        <property type="gene ID" value="NP_1360A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701126"/>
    <dbReference type="KEGG" id="nph:NP_1360A"/>
    <dbReference type="eggNOG" id="arCOG10124">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022610_0_2_2"/>
    <dbReference type="OrthoDB" id="8079at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102522">
        <property type="term" value="F:tRNA 4-demethylwyosine alpha-amino-alpha-carboxypropyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.110">
        <property type="entry name" value="Met-10+ protein-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01922">
        <property type="entry name" value="TYW2_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030382">
        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056743">
        <property type="entry name" value="TRM5-TYW2-like_MTfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056744">
        <property type="entry name" value="TRM5/TYW2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030867">
        <property type="entry name" value="TYW2_archaea"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245">
        <property type="entry name" value="TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245:SF41">
        <property type="entry name" value="TRNA(PHE) (4-DEMETHYLWYOSINE(37)-C(7)) AMINOCARBOXYPROPYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="TRM5-TYW2_MTfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25133">
        <property type="entry name" value="TYW2_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51684">
        <property type="entry name" value="SAM_MT_TRM5_TYW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1 4 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SAM-dependent methyltransferase TRM5/TYW2-type" evidence="5">
        <location>
            <begin position="95"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI48771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="345" mass="36516" checksum="1188901279A17515" modified="2005-11-08" version="1">MTDGSADADLAAIVEKPQTQTVIEALRNEGRYDPSRRIEAHGDDHVAVPVSAPPTDTAVERVEHVELPPRSRDLADLLAAHGVDAATIEAAPSSWAVIGSIILVDFGDVSAPETLDADERTLVAETLLELHANADTVLARGGVSGRCRDPDVAVVAGIGDTETVHVEHGTKYAIDFESTMFSPGNKAERARMGEVVTPGERVFDMFAGIGYFTLPMARSGATVTAAEIDPDAYRLLVENLQLNGVSDAVRPVLGDCRDVATTADRVVMGYYDAHEYLDAALSALAPDGVVHLHEATPEPAFPARPRDRLTEAAGAAGRTVEILATRTVKSHSAGVVHGVVDARVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ITE2</accession>
    <name>Q3ITE2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoesterase</fullName>
            <ecNumber evidence="1">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_1002A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallophosphoesterase superfamily. YfcE family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322227.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ITE2"/>
    <dbReference type="STRING" id="348780.NP_1002A"/>
    <dbReference type="EnsemblBacteria" id="CAI48592">
        <property type="protein sequence ID" value="CAI48592"/>
        <property type="gene ID" value="NP_1002A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701028"/>
    <dbReference type="KEGG" id="nph:NP_1002A"/>
    <dbReference type="eggNOG" id="arCOG01141">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063749_4_1_2"/>
    <dbReference type="OrthoDB" id="9959at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00841">
        <property type="entry name" value="MPP_YfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053193">
        <property type="entry name" value="MetalloPDE_YfcE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041802">
        <property type="entry name" value="MPP_YfcE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000979">
        <property type="entry name" value="Phosphodiesterase_MJ0936/Vps29"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00040">
        <property type="entry name" value="yfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43165">
        <property type="entry name" value="METALLOPHOSPHOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43165:SF1">
        <property type="entry name" value="PHOSPHODIESTERASE MJ0936"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU362039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="170" mass="18413" checksum="41AF988D4A6C9FC3" modified="2005-11-08" version="1">MHVGVISDTHDNVPAARAAMDRFEEEGVDTVVHCGDFVAPPLIPFLDRDGIAVHAVRGNNDGEREGLVDAFDALDGGTFHGRFAELTLGGREFAVLHGEERPVIDALAASGEYDYVLHGHWHVREERTVGETTVLNPGAHFPTVADEHRTVAVVDTGADEITFHAIDGER</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IU60</accession>
    <name>PDXT_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyridoxal 5'-phosphate synthase subunit PdxT</fullName>
            <ecNumber evidence="1">4.3.3.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Pdx2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Pyridoxal 5'-phosphate synthase glutaminase subunit</fullName>
            <ecNumber evidence="1">3.5.1.2</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pdxT</name>
        <name type="ordered locus">NP_0464A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + phosphate + 3 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31507"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="4.3.3.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; pyridoxal 5'-phosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">In the presence of PdxS, forms a dodecamer of heterodimers. Only shows activity in the heterodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaminase PdxT/SNO family.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.6" evidence="1"/>
    <dbReference type="EC" id="3.5.1.2" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321959.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IU60"/>
    <dbReference type="SMR" id="Q3IU60"/>
    <dbReference type="STRING" id="348780.NP_0464A"/>
    <dbReference type="EnsemblBacteria" id="CAI48323">
        <property type="protein sequence ID" value="CAI48323"/>
        <property type="gene ID" value="NP_0464A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700865"/>
    <dbReference type="KEGG" id="nph:NP_0464A"/>
    <dbReference type="eggNOG" id="arCOG00034">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069674_2_0_2"/>
    <dbReference type="OrthoDB" id="26717at2157"/>
    <dbReference type="UniPathway" id="UPA00245"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903600">
        <property type="term" value="C:glutaminase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036381">
        <property type="term" value="F:pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006543">
        <property type="term" value="P:L-glutamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008614">
        <property type="term" value="P:pyridoxine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01749">
        <property type="entry name" value="GATase1_PB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000010">
        <property type="entry name" value="uncharacterized protein LOC100176842 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01615">
        <property type="entry name" value="PdxT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002161">
        <property type="entry name" value="PdxT/SNO"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03800">
        <property type="entry name" value="PLP_synth_Pdx2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31559">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31559:SF0">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01174">
        <property type="entry name" value="SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005639">
        <property type="entry name" value="Glut_amidoT_SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51130">
        <property type="entry name" value="PDXT_SNO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000255824" description="Pyridoxal 5'-phosphate synthase subunit PdxT">
        <location>
            <begin position="1"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="53"/>
            <end position="55"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="140"/>
            <end position="141"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01615"/>
        </source>
    </evidence>
    <sequence length="192" mass="20379" checksum="67874119D4442DA3" modified="2005-11-08" version="1">MLKAGVIAVQGDVAEHADAIRRAADRHGEDCTVESIRSAGVVPECDLLLLPGGESTTISRLLAEEGIDEEIEAFAAAGKPLLATCAGLIVASRDAKDDRVSTLDILDVSVDRNAFGRQKDSFEAAIDVEGLDEPFPAVFIRAPLIDEVDAAVETLAAVDDRPVAVRQDNVVGTSFHPELTDDSRIHGLAFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ET95</accession>
    <name>A0A1U7ET95_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-L-isoaspartate(D-aspartate) O-methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.77</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">pimT2</name>
        <name evidence="11" type="ordered locus">NP_0020A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>[protein]-L-isoaspartate + S-adenosyl-L-methionine = [protein]-L-isoaspartate alpha-methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:12705"/>
            <dbReference type="Rhea" id="RHEA-COMP:12143"/>
            <dbReference type="Rhea" id="RHEA-COMP:12144"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90596"/>
            <dbReference type="ChEBI" id="CHEBI:90598"/>
            <dbReference type="EC" id="2.1.1.77"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.77" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321740.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ET95"/>
    <dbReference type="STRING" id="348780.NP_0020A"/>
    <dbReference type="EnsemblBacteria" id="CAI48101">
        <property type="protein sequence ID" value="CAI48101"/>
        <property type="gene ID" value="NP_0020A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703034"/>
    <dbReference type="KEGG" id="nph:NP_0020A"/>
    <dbReference type="eggNOG" id="arCOG00976">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055432_2_0_2"/>
    <dbReference type="OrthoDB" id="194891at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004719">
        <property type="term" value="F:protein-L-isoaspartate (D-aspartate) O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000682">
        <property type="entry name" value="PCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579:SF0">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE(D-ASPARTATE) O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01135">
        <property type="entry name" value="PCMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 11" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI48101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="246" mass="26769" checksum="C4A8CBB4C277DDF5" modified="2017-05-10" version="1">MDPAVLRDDMVDSLEHEAKAVVRSERVSVAMRAVPREPFVEDESLAYADRTGEQLGTRVLSPSAAGRLLEALDVEPDDSVLVVGAGVGYTAAVLAALTDETEVHAVDIDRRLVAAARSNLATAGYPGVLVDCRDGADGLPEYAPYDRILVEAAVVEPPRALLGQLADGGRLVAPLGNGAQELVAIEGGERVAEYGTVAFRPLLVDGERADTVERNRTRREDREHAQRAAERRRGWEQSWIDWDRHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETG7</accession>
    <name>A0A1U7ETG7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PhoU/TrkA-C domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pchB</name>
        <name evidence="2" type="ordered locus">NP_0220A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321840.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETG7"/>
    <dbReference type="STRING" id="348780.NP_0220A"/>
    <dbReference type="EnsemblBacteria" id="CAI48201">
        <property type="protein sequence ID" value="CAI48201"/>
        <property type="gene ID" value="NP_0220A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700792"/>
    <dbReference type="KEGG" id="nph:NP_0220A"/>
    <dbReference type="eggNOG" id="arCOG01963">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057266_0_0_2"/>
    <dbReference type="OrthoDB" id="85913at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="entry name" value="Phosphate transport system protein phou homolog 2, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="entry name" value="Regulator of K+ conductance, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050144">
        <property type="entry name" value="AAE_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026022">
        <property type="entry name" value="PhoU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30445">
        <property type="entry name" value="K(+)_H(+) ANTIPORTER SUBUNIT KHTT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30445:SF8">
        <property type="entry name" value="K(+)_H(+) ANTIPORTER SUBUNIT KHTT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01895">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109755">
        <property type="entry name" value="PhoU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="TrkA C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RCK C-terminal" evidence="1">
        <location>
            <begin position="117"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="1">
        <location>
            <begin position="315"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="402" mass="43341" checksum="BBE334F0496311EC" modified="2017-05-10" version="1">MTGFDEGAQPRTVDYEPRSVKDLLVEMKDTSELLIDLAYSAVLHQNDELASEVLALEEKMDVLQMRARMSLMLAVRNPKEAEALAPVLGIVAGADHVSDAAADIAKIVLDDIGLPESMRAALPEAVETLVRGTVAPASSYTGRTLEGINLESETGVRVIGIRRGDEWILNPGPETKLAAEDTVFLRGPEQRLDEVYMTLTGTEYESTEPPEPDIDDLERAVDSIILMKNLSELAVDLAYGSVLFDSDELAAEVRNLEVEVDALRSRFEAWLLGAAADADDPVALRGLIYLGVSTETISDAALDISEGVLRGLTVHPVVELAVQESDELLVRVAVEEGSDLDGTDIEAGVPVTELGMNVIAVRRPDEGWLLVSDIDLTLQPGDVLIAKGTRTSAAEFRELAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETI1</accession>
    <name>A0A1U7ETI1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DUF2270 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0240A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321850.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETI1"/>
    <dbReference type="STRING" id="348780.NP_0240A"/>
    <dbReference type="EnsemblBacteria" id="CAI48211">
        <property type="protein sequence ID" value="CAI48211"/>
        <property type="gene ID" value="NP_0240A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700799"/>
    <dbReference type="KEGG" id="nph:NP_0240A"/>
    <dbReference type="eggNOG" id="arCOG08962">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089301_0_0_2"/>
    <dbReference type="OrthoDB" id="307287at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014470">
        <property type="entry name" value="UCP01500"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10028">
        <property type="entry name" value="DUF2270"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015000">
        <property type="entry name" value="UCP01500"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="77"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="235" mass="26694" checksum="77D0B1BF8D06151E" modified="2017-05-10" version="1">MDDEHSFDPDTEEGREVAAKAGEESSDFLSLLPHYYRGEVSQMSTRISRLDLTVDWAIGLIAAVLALSFASPDSPPYFLLIGMLALTIFLGFDIRRYRSYDAARSRVRMIEENVFANAFNPNGTVHDHWRSELAEDLRKPTLKVSVREAVTRRLRRVYFPLLSLLFLAWVFRITIFVPGEQWHETASVPGIPGTVVVGIVVGYYFATAALTFWPGQRQAKGEYYGVDPGRWKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETQ1</accession>
    <name>A0A1U7ETQ1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nonhistone chromosomal protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_0424A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Protects DNA against thermal denaturation and modulates transcription.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321939.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETQ1"/>
    <dbReference type="STRING" id="348780.NP_0424A"/>
    <dbReference type="EnsemblBacteria" id="CAI48303">
        <property type="protein sequence ID" value="CAI48303"/>
        <property type="gene ID" value="NP_0424A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700851"/>
    <dbReference type="KEGG" id="nph:NP_0424A"/>
    <dbReference type="eggNOG" id="arCOG04743">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_154331_0_0_2"/>
    <dbReference type="OrthoDB" id="144708at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042262">
        <property type="term" value="P:DNA protection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.470.10">
        <property type="entry name" value="Chromosomal protein MC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008674">
        <property type="entry name" value="MC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036620">
        <property type="entry name" value="MC1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05854">
        <property type="entry name" value="MC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102875">
        <property type="entry name" value="Chromosomal protein MC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="105" mass="11920" checksum="BDDC361BEDA8A992" modified="2017-05-10" version="1">MAPDDDDDKRNFALREQNGDEPSVFSGRTPRQAALKAARRLDPAGSEDNADETELRLREKGTKKVHIYQGWAWEETAPNDSPDWMPEEITEANVSKEGIQHLEEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETR6</accession>
    <name>A0A1U7ETR6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0456A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321955.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETR6"/>
    <dbReference type="EnsemblBacteria" id="CAI48319">
        <property type="protein sequence ID" value="CAI48319"/>
        <property type="gene ID" value="NP_0456A"/>
    </dbReference>
    <dbReference type="GeneID" id="54763305"/>
    <dbReference type="KEGG" id="nph:NP_0456A"/>
    <dbReference type="eggNOG" id="arCOG11363">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_202927_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057181">
        <property type="entry name" value="DUF7859"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25258">
        <property type="entry name" value="DUF7859"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="54" mass="6060" checksum="D107467152292338" modified="2017-05-10" version="1">MSLSRPPSTALFVDVDPVLVGIITVLLLIVFGIYLFIRRTLLSLSEGMREGKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETX8</accession>
    <name>A0A1U7ETX8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Probable secreted glycoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0608A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322031.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
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                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Phosphorylates (R)-pantoate to form (R)-4-phosphopantoate in the CoA biosynthesis pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-pantoate + ATP = (R)-4-phosphopantoate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28246"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61294"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.169"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GHMP kinase family. PoK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.169" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322149.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU77"/>
    <dbReference type="STRING" id="348780.NP_0844A"/>
    <dbReference type="EnsemblBacteria" id="CAI48513">
        <property type="protein sequence ID" value="CAI48513"/>
        <property type="gene ID" value="NP_0844A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700972"/>
    <dbReference type="KEGG" id="nph:NP_0844A"/>
    <dbReference type="eggNOG" id="arCOG04263">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081191_0_0_2"/>
    <dbReference type="OrthoDB" id="85822at2157"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02223">
        <property type="entry name" value="Pantoate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012043">
        <property type="entry name" value="PoK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42282:SF1">
        <property type="entry name" value="PANTOATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42282">
        <property type="entry name" value="PANTOATE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016896">
        <property type="entry name" value="GHMP_arc_MJ0969"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="1 3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="2">
        <location>
            <begin position="67"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="272" mass="27346" checksum="EFADDB479B538FD4" modified="2017-05-10" version="1">MTDAATAFVPGHITGFFSAHPDDDPTKAGSRGAGVALDSGVTVRLEPGDGVELNGESADIEAVDRVLGALRATARVVVETDLPVSAGFGVSGGAALGTALAANAVFDRGLSENELVTIAHGAEVQAGTGLGDVVAQARGGFPVRLEPGAPAHGAMDGIPAVRDIEYVTFGELSTADVLSDNTDELTAAGQRALSSLVAEPTVETFMEASRRFAREAGLLTPPVEEAIESVADADGEASMAMLGETVFALDGGLSAAGYDATQCAVCLPGAHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU92</accession>
    <name>A0A1U7EU92_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA polymerase beta</fullName>
            <ecNumber evidence="3">2.7.7.7</ecNumber>
            <ecNumber evidence="4">4.2.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">5'-deoxyribose-phosphate lyase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">AP lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">polX</name>
        <name evidence="25" type="ordered locus">NP_0854A</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">Repair polymerase that plays a key role in base-excision repair. During this process, the damaged base is excised by specific DNA glycosylases, the DNA backbone is nicked at the abasic site by an apurinic/apyrimidic (AP) endonuclease, and POLB removes 5'-deoxyribose-phosphate from the preincised AP site acting as a 5'-deoxyribose-phosphate lyase (5'-dRP lyase); through its DNA polymerase activity, it adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. It is also able to cleave sugar-phosphate bonds 3' to an intact AP site, acting as an AP lyase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21 27">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>a 5'-end 2'-deoxyribose-2'-deoxyribonucleotide-DNA = (2E,4S)-4-hydroxypenten-2-al-5-phosphate + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:76255"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:18657"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:195194"/>
            <dbReference type="ChEBI" id="CHEBI:195195"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="3"/>
    <dbReference type="EC" id="4.2.99.18" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322154.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU92"/>
    <dbReference type="STRING" id="348780.NP_0854A"/>
    <dbReference type="EnsemblBacteria" id="CAI48518">
        <property type="protein sequence ID" value="CAI48518"/>
        <property type="gene ID" value="NP_0854A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700977"/>
    <dbReference type="KEGG" id="nph:NP_0854A"/>
    <dbReference type="eggNOG" id="arCOG00305">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017729_1_0_2"/>
    <dbReference type="OrthoDB" id="8999at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051575">
        <property type="term" value="F:5'-deoxyribose-5-phosphate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042578">
        <property type="term" value="F:phosphoric ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00141">
        <property type="entry name" value="NT_POLXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07436">
        <property type="entry name" value="PHP_PolX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.110">
        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.210.10">
        <property type="entry name" value="DNA polymerase, thumb domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002054">
        <property type="entry name" value="DNA-dir_DNA_pol_X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027421">
        <property type="entry name" value="DNA_pol_lamdba_lyase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037160">
        <property type="entry name" value="DNA_Pol_thumb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002008">
        <property type="entry name" value="DNA_pol_X_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010996">
        <property type="entry name" value="HHH_MUS81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004013">
        <property type="entry name" value="PHP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050243">
        <property type="entry name" value="PHP_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003141">
        <property type="entry name" value="Pol/His_phosphatase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029398">
        <property type="entry name" value="PolB_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022311">
        <property type="entry name" value="PolX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047967">
        <property type="entry name" value="PolX_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006375">
        <property type="entry name" value="PRK08609.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36928">
        <property type="entry name" value="PHOSPHATASE YCDX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36928:SF1">
        <property type="entry name" value="PHOSPHATASE YCDX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14791">
        <property type="entry name" value="DNA_pol_B_thumb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14520">
        <property type="entry name" value="HHH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14716">
        <property type="entry name" value="HHH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02811">
        <property type="entry name" value="PHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005047">
        <property type="entry name" value="UCP005047_YshC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00870">
        <property type="entry name" value="DNAPOLXBETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00481">
        <property type="entry name" value="POLIIIAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00483">
        <property type="entry name" value="POLXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47802">
        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="PHP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7" id="KW-0237">DNA synthesis</keyword>
    <keyword evidence="13 25" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="9 25" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0915">Sodium</keyword>
    <keyword evidence="8 25" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase X" evidence="24">
        <location>
            <begin position="1"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Helix-hairpin-helix DNA-binding motif class 1" evidence="22">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="Helix-hairpin-helix DNA-binding motif class 1" evidence="22">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Helix-hairpin-helix DNA-binding motif class 1" evidence="22">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Polymerase/histidinol phosphatase N-terminal" evidence="23">
        <location>
            <begin position="351"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00035717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00035726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00044678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00045548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="SMART" id="SM00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="CAI48518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="580" mass="61988" checksum="1DC7B7158CFD39C3" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUD5</accession>
    <name>A0A1U7EUD5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MaoC domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">maoC4</name>
        <name evidence="2" type="ordered locus">NP_0916A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322185.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUD5"/>
    <dbReference type="STRING" id="348780.NP_0916A"/>
    <dbReference type="EnsemblBacteria" id="CAI48549">
        <property type="protein sequence ID" value="CAI48549"/>
        <property type="gene ID" value="NP_0916A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702689"/>
    <dbReference type="KEGG" id="nph:NP_0916A"/>
    <dbReference type="eggNOG" id="arCOG00775">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094876_0_1_2"/>
    <dbReference type="OrthoDB" id="209979at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03451">
        <property type="entry name" value="FkbR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048274">
        <property type="entry name" value="MC_hydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052342">
        <property type="entry name" value="MCH/BMMD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43664:SF1">
        <property type="entry name" value="BETA-METHYLMALYL-COA DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43664">
        <property type="entry name" value="MONOAMINE OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19315">
        <property type="entry name" value="MC_hydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="210" mass="23162" checksum="9229FEF78AA3B18F" modified="2017-05-10" version="1">MERSDGDHGPVDGDAAADDRRLVAGWQGRYYEDFAVGDVYKHPYGRTVTETDNVWLTNLTMNVNPMHFNAAYASATEFGERLVDGLYIIALAVGMSVADVSANATANLGYDRVRHHAPVFHGDTIFAESEVLEKRESDSRAHVGIVTTELRAYNQDDELVLSLERTPMVLKREAAEPSAARPTGWPDGVGTQPEDVAGEVVGRRRREDRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUD9</accession>
    <name>A0A1U7EUD9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Rhodanese domain protein / beta-lactamase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0980A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322216.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUD9"/>
    <dbReference type="STRING" id="348780.NP_0980A"/>
    <dbReference type="EnsemblBacteria" id="CAI48581">
        <property type="protein sequence ID" value="CAI48581"/>
        <property type="gene ID" value="NP_0980A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701018"/>
    <dbReference type="KEGG" id="nph:NP_0980A"/>
    <dbReference type="eggNOG" id="arCOG00517">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030571_7_2_2"/>
    <dbReference type="OrthoDB" id="9180at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050313">
        <property type="term" value="F:sulfur dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070813">
        <property type="term" value="P:hydrogen sulfide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07724">
        <property type="entry name" value="POD-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051682">
        <property type="entry name" value="Mito_Persulfide_Diox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044528">
        <property type="entry name" value="POD-like_MBL-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43084">
        <property type="entry name" value="PERSULFIDE DIOXYGENASE ETHE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43084:SF1">
        <property type="entry name" value="PERSULFIDE DIOXYGENASE ETHE1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="29"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="399" mass="43177" checksum="14F1FD4531470E86" modified="2017-05-10" version="1">MDPEAMDFPTPDATVESTTPRELKERIDAGEPVTLLDARMESDYEEWRIDGDTVESINIPYFEFLDEDIDDDVLASVPDDEHVTVLCAKGGASEYVAGALADRGYDVDHLADGMNGWASIYEPHEVTGYDGAGTLLQYQRPSSGCLGYFLYHDGEAAVIDPLRAFTDRYLDDADELGVELRYAFDTHVHADHVSGLRDLAAEGVEAVLPGPAADRGVTYTDEVTLTQDGDTFPVGEATIETVFTPGHTTGMTSYLVDEGFLATGDGLFIESVARPDLEEGDEGAPDAARTLYQSLQERVRTLPDETLIGGAHFSDAAAPASDGTYTATLGALDSRMDALSMDEDEFVDLVLSDMPPRPANYEDIIETNLGQREIDDEEAFTLELGPNNCAASQDALTGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUF0</accession>
    <name>A0A1U7EUF0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DUF502 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0986A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322219.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUF0"/>
    <dbReference type="STRING" id="348780.NP_0986A"/>
    <dbReference type="EnsemblBacteria" id="CAI48584">
        <property type="protein sequence ID" value="CAI48584"/>
        <property type="gene ID" value="NP_0986A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701021"/>
    <dbReference type="KEGG" id="nph:NP_0986A"/>
    <dbReference type="eggNOG" id="arCOG04755">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068050_3_0_2"/>
    <dbReference type="OrthoDB" id="156682at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007462">
        <property type="entry name" value="COV1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31876">
        <property type="entry name" value="COV-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31876:SF26">
        <property type="entry name" value="PROTEIN LIKE COV 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04367">
        <property type="entry name" value="DUF502"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="214" mass="23339" checksum="DC85ADF154C0D92E" modified="2017-05-10" version="1">MLSSSWKRDIASGLIVLVPLLVTAYVVAFIYQAIANLPLFEEALGGLPVAVRVVVTLVLFVLLVLSVGYLMRTTAGDLLEEALDNVMNRLPGLRVVYNASKMAVETAVSGTDDLQTPVKLETWNGMRMTAFKTGQQTEDGRDMLFLPTAPNITTGFVIEVEPDRYTELDESVEEALTRVLSAGFGERNTQSIQIDVTEGPPTAKTEKRPSSDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUN9</accession>
    <name>A0A1U7EUN9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable S-adenosylmethionine-dependent methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1178A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322316.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUN9"/>
    <dbReference type="STRING" id="348780.NP_1178A"/>
    <dbReference type="EnsemblBacteria" id="CAI48680">
        <property type="protein sequence ID" value="CAI48680"/>
        <property type="gene ID" value="NP_1178A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701076"/>
    <dbReference type="KEGG" id="nph:NP_1178A"/>
    <dbReference type="eggNOG" id="arCOG01794">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102820_0_0_2"/>
    <dbReference type="OrthoDB" id="190449at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="1">
        <location>
            <begin position="49"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="236" mass="26712" checksum="72A24E5C669C522D" modified="2017-05-10" version="1">MKGQEWYQEDDIAEAYDDKRFSGGGRYIDRREKEAVLDALAPVDGKRILEVACGTGRFTVMLAERGADIVGMDISEAMLEQGRAKARAAGVEETLSFLQGDAGRLPFPDNHFDAVFAMRFFHLAPDPEGFIKEMRRVTKDQIFFDTFKRYSARSIYNWALPMGSRLYSTREAKELVVGADLELADAAHDFIVPYGFYRAVPRWLARPFRMADTTIGRTTAGDRLASVSYWDARIPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUS6</accession>
    <name>A0A1U7EUS6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Thioredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">trxA1</name>
        <name evidence="6" type="ordered locus">NP_1310A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the thioredoxin family. Plant H-type subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322381.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUS6"/>
    <dbReference type="STRING" id="348780.NP_1310A"/>
    <dbReference type="EnsemblBacteria" id="CAI48746">
        <property type="protein sequence ID" value="CAI48746"/>
        <property type="gene ID" value="NP_1310A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703422"/>
    <dbReference type="KEGG" id="nph:NP_1310A"/>
    <dbReference type="eggNOG" id="arCOG01972">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090389_10_4_2"/>
    <dbReference type="OrthoDB" id="35385at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005746">
        <property type="entry name" value="Thioredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050620">
        <property type="entry name" value="Thioredoxin_H-type-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10438">
        <property type="entry name" value="THIOREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10438:SF468">
        <property type="entry name" value="THIOREDOXIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000077">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="5">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="13"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="16"/>
        </location>
    </feature>
    <feature type="site" description="Deprotonates C-terminal active site Cys" evidence="3">
        <location>
            <position position="7"/>
        </location>
    </feature>
    <feature type="site" description="Contributes to redox potential value" evidence="3">
        <location>
            <position position="14"/>
        </location>
    </feature>
    <feature type="site" description="Contributes to redox potential value" evidence="3">
        <location>
            <position position="15"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="4">
        <location>
            <begin position="13"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000077-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000077-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI48746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="88" mass="9828" checksum="73692C89A137ECC0" modified="2017-05-10" version="1">MSVTLKDFYADWCGPCKTQDPILEDLEADYPDVDFEKVDVDAAQDVANEYQVRSLPTLIVENDDGIVERFVGVTQRDDLEDALDEAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUT3</accession>
    <name>A0A1U7EUT3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oxidoreductase (Homolog to zinc-containing alcohol dehydrogenase / threonine 3-dehydrogenase)</fullName>
            <ecNumber evidence="7">1.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">NP_1340A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.-" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322396.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUT3"/>
    <dbReference type="STRING" id="348780.NP_1340A"/>
    <dbReference type="EnsemblBacteria" id="CAI48761">
        <property type="protein sequence ID" value="CAI48761"/>
        <property type="gene ID" value="NP_1340A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701119"/>
    <dbReference type="KEGG" id="nph:NP_1340A"/>
    <dbReference type="eggNOG" id="arCOG01621">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_9_0_2"/>
    <dbReference type="OrthoDB" id="168897at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08255">
        <property type="entry name" value="2-desacetyl-2-hydroxyethyl_bacteriochlorophyllide_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43350:SF19">
        <property type="entry name" value="D-GULOSIDE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43350">
        <property type="entry name" value="NAD-DEPENDENT ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="6">
        <location>
            <begin position="162"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="338" mass="36588" checksum="9A04410F7CF50ACD" modified="2017-05-10" version="1">MSTTDGRAVQFIGPEDVDVVPLQSPEPDPEGVVVETLVSAISAGTELLVYHGDVDSETLADESLPALDGTLSYPVRYGYSAVGRVVATGAEVAPAWHDRTVFAFNPHESHFRAEPEALHVVPEGVSPEQAALFANVETAVNFALDASPRLGERVAVFGQGVVGLLTTALLSELPLETLVAVEPHPFRRQLAARMGADETVDPTAENAAAAVEELTDGVDVAIEVSGQPQTLESAVEATRYDGRVLVGSWYGTKREPLSLGDHYHRGRISIESSQVSTIDPSLRGRWDRTRRRKTAWRRLQSLDLGPLITHRVDVAEAGSAYRQLTEHPNETLQVLLTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUV0</accession>
    <name>A0A1U7EUV0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sec-independent protein translocase protein TatC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tatC2</name>
        <name evidence="5" type="synonym">tatC</name>
        <name evidence="7" type="ordered locus">NP_1350A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Forms a complex with TatA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TatC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322401.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUV0"/>
    <dbReference type="STRING" id="348780.NP_1350A"/>
    <dbReference type="EnsemblBacteria" id="CAI48766">
        <property type="protein sequence ID" value="CAI48766"/>
        <property type="gene ID" value="NP_1350A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703228"/>
    <dbReference type="KEGG" id="nph:NP_1350A"/>
    <dbReference type="eggNOG" id="arCOG04736">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_359694_0_0_2"/>
    <dbReference type="OrthoDB" id="15305at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033281">
        <property type="term" value="C:TAT protein transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009977">
        <property type="term" value="F:proton motive force dependent protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043953">
        <property type="term" value="P:protein transport by the Tat complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00902">
        <property type="entry name" value="TatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002033">
        <property type="entry name" value="TatC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30371">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30371:SF0">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATC, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00902">
        <property type="entry name" value="TatC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01840">
        <property type="entry name" value="TATCFAMILY"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="34"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="121"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="207"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="272"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="322"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="500"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="552"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="592"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="644"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="681"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="703"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="422"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="422"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="741" mass="80337" checksum="E9BD172554679755" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUW2</accession>
    <name>A0A1U7EUW2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1406A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322429.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUW2"/>
    <dbReference type="EnsemblBacteria" id="CAI48794">
        <property type="protein sequence ID" value="CAI48794"/>
        <property type="gene ID" value="NP_1406A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701140"/>
    <dbReference type="KEGG" id="nph:NP_1406A"/>
    <dbReference type="eggNOG" id="arCOG04723">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104046_0_0_2"/>
    <dbReference type="OrthoDB" id="338444at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043868">
        <property type="entry name" value="DUF5828"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19146">
        <property type="entry name" value="DUF5828"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="235" mass="26284" checksum="90BCFD0A59129BDA" modified="2017-05-10" version="1">MDIEESVSGFKARGGWGDIVEHGERITRALRELDAEEDHDVDGDALEEWDEWRPKADERLGEDVSRKTAEQASVGEGEGEKAGKDPDEDLKTAGEKLSDSYESLEEPGEAVDEWRESINYVARAADSATRKALRKVEGSVYRNVMTQMSPYYFDNELVSANLTRGDSAADDYIFEVNINDDDLKIRVSNKLADYQTSVERWHVDTPKDTDIAEAVEGHEPPQDEPTDGGSDPKRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUZ2</accession>
    <name>A0A1U7EUZ2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type transport system ATP-binding protein (Probable substrate branched-chain amino acids)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">livF3</name>
        <name evidence="7" type="synonym">abc07a1</name>
        <name evidence="7" type="ordered locus">NP_1472A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322462.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUZ2"/>
    <dbReference type="STRING" id="348780.NP_1472A"/>
    <dbReference type="EnsemblBacteria" id="CAI48827">
        <property type="protein sequence ID" value="CAI48827"/>
        <property type="gene ID" value="NP_1472A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702382"/>
    <dbReference type="KEGG" id="nph:NP_1472A"/>
    <dbReference type="eggNOG" id="arCOG00924">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OrthoDB" id="97750at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015807">
        <property type="term" value="P:L-amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03224">
        <property type="entry name" value="ABC_TM1139_LivF_branched"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052156">
        <property type="entry name" value="BCAA_Transport_ATP-bd_LivF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43820">
        <property type="entry name" value="HIGH-AFFINITY BRANCHED-CHAIN AMINO ACID TRANSPORT ATP-BINDING PROTEIN LIVF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43820:SF4">
        <property type="entry name" value="HIGH-AFFINITY BRANCHED-CHAIN AMINO ACID TRANSPORT ATP-BINDING PROTEIN LIVF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="2"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="234" mass="25909" checksum="62619C0FFD50EBAF" modified="2017-05-10" version="1">MLEVDNVSISYGKTSAVSDVSLTVEEGEIVGIIGPNGAGKTTLLDAISGFKGYEGSIRFGGEEIRGLTEQEIVQRGLVYCTEDRDLFPFFSVHENLLMGAQFREDRDAVQEDLEFVYDLFPRLDERRDQHAETMSGGEQQMLAVGRSLMSDPDLLMLDEPTLGLAPVIIEDIKEAIERLSDAGLTILLAEQNSTFALDHAERLCLLERGEITRQGDAETLKNDDYVRDAYIGVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV10</accession>
    <name>A0A1U7EV10_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NP_1176A family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1522A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV10"/>
    <dbReference type="STRING" id="348780.NP_1522A"/>
    <dbReference type="EnsemblBacteria" id="CAI48852">
        <property type="protein sequence ID" value="CAI48852"/>
        <property type="gene ID" value="NP_1522A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_1522A"/>
    <dbReference type="eggNOG" id="arCOG00394">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_176006_1_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="111" mass="12263" checksum="896489B0DB1B6524" modified="2017-05-10" version="1">MSATDTKAVESDGWEAVAELPPSAKLVAKVLEYNDRLTQSQLAEETMLPPRTVRYGLNRLEEVDAVDSRFSFTDARKRVYTLAVEDEAKQDGTRGTKRDGTRSGSVLPADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV31</accession>
    <name>A0A1U7EV31_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">HTH-10 family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_1532A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322491.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV31"/>
    <dbReference type="STRING" id="348780.NP_1532A"/>
    <dbReference type="EnsemblBacteria" id="CAI48857">
        <property type="protein sequence ID" value="CAI48857"/>
        <property type="gene ID" value="NP_1532A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701172"/>
    <dbReference type="KEGG" id="nph:NP_1532A"/>
    <dbReference type="eggNOG" id="arCOG02274">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101251_0_0_2"/>
    <dbReference type="OrthoDB" id="51502at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007050">
        <property type="entry name" value="HTH_bacterioopsin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236:SF1">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04967">
        <property type="entry name" value="HTH_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH bat-type" evidence="3">
        <location>
            <begin position="151"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="219" mass="23551" checksum="6243E6C5785E6F58" modified="2017-05-10" version="1">MTLARIRVDLPGDAWVSTVSRSFPDATFRLLATMPGADHGEAVVSVEADRPEAVLDAMADCDPDHEPSPVSQTDRGLTVQLDTAQPEPLRAASESGLPVEPPCRIQNGTATVDLRGSHDRLSAFGQQLDAAGLDYEVEFVGGFEDGADRLLTDTQRELLMTAIDLGYYDTPRGCTLTELAAHLDIAKSTCSETLQRAEGNLIKQFAREHFSAPAQPASD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV33</accession>
    <name>A0A1U7EV33_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Alternative heme synthase AhbD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ahbD</name>
        <name evidence="8" type="synonym">cmo1</name>
        <name evidence="8" type="ordered locus">NP_1546A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322498.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV33"/>
    <dbReference type="STRING" id="348780.NP_1546A"/>
    <dbReference type="EnsemblBacteria" id="CAI48864">
        <property type="protein sequence ID" value="CAI48864"/>
        <property type="gene ID" value="NP_1546A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702614"/>
    <dbReference type="KEGG" id="nph:NP_1546A"/>
    <dbReference type="eggNOG" id="arCOG00938">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009273_4_0_2"/>
    <dbReference type="OrthoDB" id="30736at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21123">
        <property type="entry name" value="SPASM_MftC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017200">
        <property type="entry name" value="PqqE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050377">
        <property type="entry name" value="Radical_SAM_PqqE_MftC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04053">
        <property type="entry name" value="TIGR04053 family radical SAM/SPASM domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228">
        <property type="entry name" value="RADICAL SAM DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228:SF34">
        <property type="entry name" value="TUNGSTEN-CONTAINING ALDEHYDE FERREDOXIN OXIDOREDUCTASE COFACTOR MODIFYING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037420">
        <property type="entry name" value="PQQ_syn_pqqE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01386">
        <property type="entry name" value="main_SPASM_domain-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="16"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI48864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="371" mass="39699" checksum="E9D22F0F13A413D7" modified="2017-05-10" version="1">MSTGAPIPSPRDIDTSQRPFVLIWEVTQACELACDHCRADAKPSRHPDELTTAEGKRLLDSAAEFGDGQLVVLSGGDPMKRDDLVELVRYGTKQGLRMTLTPSGTEALTPDNIAALVDAGLQRMALSVDAPATAHDRFRGEAGSFERTREAAEAARDAGIPLQTNTTVCATTVETLPAVREFVAEVGAVLWSVFFLVPVGRGQILDPIQPERAESVLEWLQDVSEAAPFGVKTTEAPHYRRVALQRNGSATDAPDADAIGRRGGIIAGDGFAFVSHTGELFPSGFLPKSAGNVTDSDLVSLYQDSTLFESLRDRDRLKGKCGACEFRHVCGGSRSRAYAVTGDPLASDPLCAYRPSGYDGPLPDRQAAADR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV60</accession>
    <name>A0A1U7EV60_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">LysE family transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tp10</name>
        <name evidence="7" type="ordered locus">NP_1634A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322542.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV60"/>
    <dbReference type="STRING" id="348780.NP_1634A"/>
    <dbReference type="EnsemblBacteria" id="CAI48908">
        <property type="protein sequence ID" value="CAI48908"/>
        <property type="gene ID" value="NP_1634A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703286"/>
    <dbReference type="KEGG" id="nph:NP_1634A"/>
    <dbReference type="eggNOG" id="arCOG01947">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087840_1_2_2"/>
    <dbReference type="OrthoDB" id="121309at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001123">
        <property type="entry name" value="LeuE-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38825">
        <property type="entry name" value="LYSINE EXPORTER PROTEIN (LYSE/YGGA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38825:SF2">
        <property type="entry name" value="LYSINE TRANSPORTER LYSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01810">
        <property type="entry name" value="LysE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="216" mass="22096" checksum="7287A0554B0ECF17" modified="2017-05-10" version="1">MSLLTTLLAGVLFGLALAAPPGPMNAIIAEESVQRGWTAGFRAGLGAMAADVLFFGLAVVGLVAVVDRSPTLQRGLFLVGGLLMLYFAVGAARSADGQFDGTTPDRSRGFSKTFLLSLTNPYQILFWLTAGVGLLTPGVVDVLAAATERLSGLVVVETGHPALVAGLFGGVAAWILVFPAALVAARSRIDVLAPAIAYGSAAVLGGFGLYFLIAAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV68</accession>
    <name>A0A1U7EV68_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Acyl-CoA dehydrogenase</fullName>
            <ecNumber evidence="10">1.3.8.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">acd5</name>
        <name evidence="10" type="ordered locus">NP_1596A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.-" evidence="10"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322523.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV68"/>
    <dbReference type="STRING" id="348780.NP_1596A"/>
    <dbReference type="EnsemblBacteria" id="CAI48889">
        <property type="protein sequence ID" value="CAI48889"/>
        <property type="gene ID" value="NP_1596A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702480"/>
    <dbReference type="KEGG" id="nph:NP_1596A"/>
    <dbReference type="eggNOG" id="arCOG01707">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_0_2_2"/>
    <dbReference type="OrthoDB" id="275197at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01158">
        <property type="entry name" value="SCAD_SBCAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000009">
        <property type="entry name" value="Acyl-CoA dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000002">
        <property type="entry name" value="Acyl-CoA dehydrogenase FadE19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000004">
        <property type="entry name" value="Acyl-CoA dehydrogenase FadE25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF12">
        <property type="entry name" value="ISOVALERYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016578">
        <property type="entry name" value="HsaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0274">FAD</keyword>
    <keyword evidence="3 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 6 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="9">
        <location>
            <begin position="6"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="8">
        <location>
            <begin position="122"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="7">
        <location>
            <begin position="230"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI48889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="381" mass="41418" checksum="F97DBBE7DAB2B815" modified="2017-05-10" version="1">MDFALTAEQRQIKDMVSEFVDEEVVPVADELDKNDEFPWDLVDELAELGLMGMPFPEEYGGAGLDYHAYPAALEEIARGSGGLGTIVAAHISLAGNMVYKFGNDEQKEEYLTPLAQGEEIGAFALSEAGAGSDVPAMGTTAEKDGDEYVINGDKLWISNGSVADTVTVFAKTDPEAGNKGISSFIVRPDTDDGFYVEGTEDKLGDKGCPTAELRFDDLRIPESRLLGDEGRGFVQALKTLNGGRITIAARSVGIAQAALDEAADYAQDREQFDQPISDFQAIQHKLADMDTKTRAARLLMHDAADKKIRGESFAKEAAQAKLYASEVSREVANEGIQIHGGYGYTKDFPAERFYRDAKLNEIYEGTSEVLRNTIAAQLLEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVB4</accession>
    <name>A0A1U7EVB4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1680A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVB4"/>
    <dbReference type="EnsemblBacteria" id="CAI48931">
        <property type="protein sequence ID" value="CAI48931"/>
        <property type="gene ID" value="NP_1680A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_1680A"/>
    <dbReference type="eggNOG" id="arCOG06816">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_914043_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="130"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="304" mass="32712" checksum="AA4212AA4AD1F538" modified="2017-05-10" version="1">MATRRRFMCMGSTTVGLVALSGCLGAEGAEEVDTAMELLDENFEQFEAFDERDDLPDEAALEAIETRLDEAESHLEDAAAVADDDEVLAAIELGGAFVAFQRALVRVVGGFVELEHGMDSFDAYIDAERFDDAIAEAELLQTTVDELEAAVDDALAAIDAVDDSQLEAEDRISVTLTKSDLERIASEASALEEVIGGLGDLADGLQAITDGFEALEAEDFDTARDAFSAAEEELAPVVDRYTRLEDDADLPAHMEADVIGLSCVAEAMRDAATLAEEGATAGMNEEWDTFDDRLEQAEEAMDRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVD1</accession>
    <name>A0A1U7EVD1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Glutamate synthase large subunit</fullName>
            <ecNumber evidence="19">1.4.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">gltB</name>
        <name evidence="19" type="ordered locus">NP_1794A</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="16">Amino-acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glutamate synthase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.-.-" evidence="19"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322620.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="STRING" id="348780.NP_1794A"/>
    <dbReference type="EnsemblBacteria" id="CAI48988">
        <property type="protein sequence ID" value="CAI48988"/>
        <property type="gene ID" value="NP_1794A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702760"/>
    <dbReference type="KEGG" id="nph:NP_1794A"/>
    <dbReference type="eggNOG" id="arCOG04553">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_8_2_2"/>
    <dbReference type="OrthoDB" id="211693at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015930">
        <property type="term" value="F:glutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019676">
        <property type="term" value="P:ammonia assimilation cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00982">
        <property type="entry name" value="gltB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00713">
        <property type="entry name" value="GltS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02808">
        <property type="entry name" value="GltS_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.60:FF:000001">
        <property type="entry name" value="Glutamate synthase, large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000001">
        <property type="entry name" value="Glutamate synthase, large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.60">
        <property type="entry name" value="Glutamate synthase, alpha subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050711">
        <property type="entry name" value="ET-N_metabolism_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002489">
        <property type="entry name" value="Glu_synth_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036485">
        <property type="entry name" value="Glu_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006982">
        <property type="entry name" value="Glu_synth_centr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002932">
        <property type="entry name" value="Glu_synthdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008730">
        <property type="entry name" value="PRK11750.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11938">
        <property type="entry name" value="FAD NADPH DEHYDROGENASE/OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11938:SF133">
        <property type="entry name" value="GLUTAMATE SYNTHASE (NADH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00310">
        <property type="entry name" value="GATase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04898">
        <property type="entry name" value="Glu_syn_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01645">
        <property type="entry name" value="Glu_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01493">
        <property type="entry name" value="GXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69336">
        <property type="entry name" value="Alpha subunit of glutamate synthase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0288">FMN</keyword>
    <keyword evidence="14" id="KW-0314">Glutamate biosynthesis</keyword>
    <keyword evidence="10" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="18">
        <location>
            <begin position="23"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1478"/>
            <end position="1508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1487"/>
            <end position="1501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAI48988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="1508" mass="164708" checksum="8B5D31AED14CD495" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVK4</accession>
    <name>A0A1U7EVK4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyltransferase AglG</fullName>
            <ecNumber evidence="3">2.4.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">aglG</name>
        <name evidence="3" type="synonym">gtl2</name>
        <name evidence="3" type="ordered locus">NP_1916A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.4.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322681.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVK4"/>
    <dbReference type="STRING" id="348780.NP_1916A"/>
    <dbReference type="EnsemblBacteria" id="CAI49049">
        <property type="protein sequence ID" value="CAI49049"/>
        <property type="gene ID" value="NP_1916A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702779"/>
    <dbReference type="KEGG" id="nph:NP_1916A"/>
    <dbReference type="eggNOG" id="arCOG01387">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025996_19_2_2"/>
    <dbReference type="OrthoDB" id="324632at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00761">
        <property type="entry name" value="Glyco_tranf_GTA_type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053553">
        <property type="entry name" value="GDP_glucuronosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050834">
        <property type="entry name" value="Glycosyltransf_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041394">
        <property type="entry name" value="GtaseAglG_Halo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43685">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43685:SF2">
        <property type="entry name" value="GLYCOSYLTRANSFERASE 2-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="277"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="2">
        <location>
            <begin position="4"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="308" mass="34658" checksum="39A65369698BE563" modified="2017-05-10" version="1">MDVSVVVCVYSMDRFESFVEAVESVLAQTYDPIEAILVVDGNQAVYERVADRFGDRDDVVVHCNEENRGVSVSRTHGAELASGDVVAFIDDDAVAEPDWIEHLVEAYEKHDAVAVGGRMTGEWLAGRPSFLPDEFDWLVGVTHRGFADGRTEVRNTFESNISFRREVFLDLGGFDPELGPTGEEYRHSEGAEIGARLEAEYDRGVMYIPEAIVRHKVFEHRTRLSWLCRRAFEQGVSKRAMKRRTDGSSAEEFGFLRSLFLEHLPRRLRELIDEPSVAGVAQIVMLFVFTGLVGVGYLFESAWGIVSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVL5</accession>
    <name>A0A1U7EVL5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphate transport system permease protein PstA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">pstA1</name>
        <name evidence="10" type="synonym">abc14p1</name>
        <name evidence="10" type="ordered locus">NP_1982A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322712.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVL5"/>
    <dbReference type="STRING" id="348780.NP_1982A"/>
    <dbReference type="EnsemblBacteria" id="CAI49082">
        <property type="protein sequence ID" value="CAI49082"/>
        <property type="gene ID" value="NP_1982A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702412"/>
    <dbReference type="KEGG" id="nph:NP_1982A"/>
    <dbReference type="eggNOG" id="arCOG00168">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033621_2_2_2"/>
    <dbReference type="OrthoDB" id="11402at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005672">
        <property type="entry name" value="Phosphate_PstA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00974">
        <property type="entry name" value="3a0107s02c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43470:SF5">
        <property type="entry name" value="PHOSPHATE TRANSPORT SYSTEM PERMEASE PROTEIN PSTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43470">
        <property type="entry name" value="PHOSPHATE TRANSPORT SYSTEM PERMEASE PROTEIN PSTA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="81"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="156"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="268"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="85"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI49082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="301" mass="31860" checksum="617454AC4BCCED9D" modified="2017-05-10" version="1">MAADDRLADLTAGDDLARKRLYGRVFVAVCLISTLVGVVALGLLVADLIYEARGWLTIDFLTHPPSWFPENYQPGGRGAGLYPAIIGSVFLIALTALFTIFLGVGAAIYLEEYAPDNRLTRFIEANIANLAGVPSIVYGLLGLAIFVRAAGLGSSLLAGALTLTLLILPIVIVSAQEAIRAVPDSRRQAAYGVGATKWQVTRDVVLPSALPGIMTGTILSLSRAIGETAPILMVGAATFMIAPPDSLLGPFSAMPMQVFEWAKQPDPAFQHIAAAGSIVLLTVLVLMNATAILIRNKYDTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVQ6</accession>
    <name>A0A1U7EVQ6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flagellin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">flg1</name>
        <name evidence="7" type="ordered locus">NP_2086A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Flagellin is the subunit protein which polymerizes to form the filaments of archaeal flagella.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Archaeal flagellum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the archaeal flagellin family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322762.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVQ6"/>
    <dbReference type="STRING" id="348780.NP_2086A"/>
    <dbReference type="EnsemblBacteria" id="CAI49134">
        <property type="protein sequence ID" value="CAI49134"/>
        <property type="gene ID" value="NP_2086A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702718"/>
    <dbReference type="KEGG" id="nph:NP_2086A"/>
    <dbReference type="eggNOG" id="arCOG01829">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051124_1_0_2"/>
    <dbReference type="OrthoDB" id="102632at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097589">
        <property type="term" value="C:archaeal-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097588">
        <property type="term" value="P:archaeal or bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013373">
        <property type="entry name" value="Flagellin/pilin_N_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002774">
        <property type="entry name" value="Flagellin_arc-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02537">
        <property type="entry name" value="arch_flag_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35903">
        <property type="entry name" value="FLAGELLIN B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35903:SF1">
        <property type="entry name" value="FLAGELLIN B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01917">
        <property type="entry name" value="Flagellin_arch-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0974">Archaeal flagellum</keyword>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="7" id="KW-0282">Flagellum</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="200"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="215" mass="22720" checksum="CE23CAD9DD1CCA9F" modified="2017-05-10" version="1">MFETLTETKERGQVGIGTLIVFIALVLVAAIAAGVLINTAGFLQTQAQATGEESTEQVSTNLVYLSTTGEVVEDGTGSLVGIDQFETTVQLGPGSSAIDLNDTTISVFTDDGESEEFDGVDEGDSLTDDGIEFDDTDRDSSVLSTDATDDDATATIVFGLDDASDGINPEDDRFDEGTTVDIVITTAEGTQAENSFIIEDPLDPQLDDEDEIRLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVT3</accession>
    <name>A0A1U7EVT3_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Profilin fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2124A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322781.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVT3"/>
    <dbReference type="STRING" id="348780.NP_2124A"/>
    <dbReference type="EnsemblBacteria" id="CAI49153">
        <property type="protein sequence ID" value="CAI49153"/>
        <property type="gene ID" value="NP_2124A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701357"/>
    <dbReference type="KEGG" id="nph:NP_2124A"/>
    <dbReference type="eggNOG" id="arCOG08116">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1140594_0_0_2"/>
    <dbReference type="OrthoDB" id="200571at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058872">
        <property type="entry name" value="Halo_prof"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26420">
        <property type="entry name" value="Halo_prof"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Profilin fold" evidence="1">
        <location>
            <begin position="18"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Profilin fold" evidence="1">
        <location>
            <begin position="136"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="243" mass="26568" checksum="EED894D493C37314" modified="2017-05-10" version="1">MAGEGLDIDIDITDIAAVADAREQLLAAVADHAGEIAADIARLDGGDYGSRSFSTDRGQWTLKHERGTLQYLRYEPQGGGETYVVSTQRPAAPEPLSDALADYDAFVTAYNDYVDRVAGLLDDVDDEFPAVRSTADVAAERDRIVDGIERACHRMAEGLYRYEGTEYGTFSARVDSTRWELKRETERVSYLRVGGSNGIYLLSQYGQPSAADVRSHAPEFGGFVAAYNDHVAELAADLETIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVU6</accession>
    <name>A0A1U7EVU6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chemotaxis protein CheA</fullName>
            <ecNumber evidence="3">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">cheA</name>
        <name evidence="18" type="ordered locus">NP_2172A</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322805.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVU6"/>
    <dbReference type="STRING" id="348780.NP_2172A"/>
    <dbReference type="EnsemblBacteria" id="CAI49177">
        <property type="protein sequence ID" value="CAI49177"/>
        <property type="gene ID" value="NP_2172A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702597"/>
    <dbReference type="KEGG" id="nph:NP_2172A"/>
    <dbReference type="eggNOG" id="arCOG04403">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000650_3_6_2"/>
    <dbReference type="OrthoDB" id="293137at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16916">
        <property type="entry name" value="HATPase_CheA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00088">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000016">
        <property type="entry name" value="Chemotaxis protein CheA, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.560">
        <property type="entry name" value="Histidine kinase CheA-like, homodimeric domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.160">
        <property type="entry name" value="HPT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051315">
        <property type="entry name" value="Bact_Chemotaxis_CheA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004105">
        <property type="entry name" value="CheA-like_dim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037006">
        <property type="entry name" value="CheA-like_homodim_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010808">
        <property type="entry name" value="CheA_P2-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036061">
        <property type="entry name" value="CheW-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002545">
        <property type="entry name" value="CheW-lke_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035891">
        <property type="entry name" value="CheY-binding_CheA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036641">
        <property type="entry name" value="HPT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008207">
        <property type="entry name" value="Sig_transdc_His_kin_Hpt_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43395:SF10">
        <property type="entry name" value="CHEMOTAXIS PROTEIN CHEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43395">
        <property type="entry name" value="SENSOR HISTIDINE KINASE CHEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01584">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02895">
        <property type="entry name" value="H-kinase_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01627">
        <property type="entry name" value="Hpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07194">
        <property type="entry name" value="P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00260">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01231">
        <property type="entry name" value="H-kinase_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00073">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50341">
        <property type="entry name" value="CheW-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55052">
        <property type="entry name" value="CheY-binding domain of CheA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47226">
        <property type="entry name" value="Histidine-containing phosphotransfer domain, HPT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50851">
        <property type="entry name" value="CHEW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50894">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 13" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="HPt" evidence="17">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="15">
        <location>
            <begin position="392"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="CheW-like" evidence="16">
        <location>
            <begin position="610"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="127"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="263"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="137"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="271"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="298"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="340"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphohistidine" evidence="13">
        <location>
            <position position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAI49177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="759" mass="81125" checksum="CC31A8F61776C17C" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW11</accession>
    <name>A0A1U7EW11_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">CBS/parB domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cbs10</name>
        <name evidence="5" type="ordered locus">NP_2318A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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    <dbReference type="RefSeq" id="WP_011322877.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A1U7EW11"/>
    <dbReference type="STRING" id="348780.NP_2318A"/>
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        <property type="gene ID" value="NP_2318A"/>
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    <dbReference type="GeneID" id="3701404"/>
    <dbReference type="KEGG" id="nph:NP_2318A"/>
    <dbReference type="eggNOG" id="arCOG00622">
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    <dbReference type="HOGENOM" id="CLU_982154_0_0_2"/>
    <dbReference type="OrthoDB" id="89900at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd04610">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1530.10">
        <property type="entry name" value="Conserved hypothetical protein from pyrococcus furiosus pfu- 392566-001, ParB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003115">
        <property type="entry name" value="ParB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016427">
        <property type="entry name" value="UCP004699_CBS/ParB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004699">
        <property type="entry name" value="UCP004699_CBS_ParB"/>
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    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00470">
        <property type="entry name" value="ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="ParB/Sulfiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="20"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="79"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="271" mass="30079" checksum="497F10DDDB9248B9" modified="2017-05-10" version="1">MDAPDDGLDSRDKPKVDDYMTRDVATVSPDDTVEDVAQRIADSDSHSGYPVCDGRTVDGFVSARDLLLAEDHEPIFKVMTEDIIVAHPDMKLTDAARVILRSGIQQLPVVDDAGNLVGIISNADVIRSQIERATPEKVGKLKETLENIHGVEATEDRRTVELNSLTPTQTKVYTDELEGRSYELENGLAEPLVVIDNAGELLLADGHHRVKAASRLGIEEMDAYVIVLDRRVELGMAETARKCDLERIEDIQEVDYAHHPLVETTERLQEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW26</accession>
    <name>A0A1U7EW26_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DHH/RecJ family phosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2316A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322876.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW26"/>
    <dbReference type="STRING" id="348780.NP_2316A"/>
    <dbReference type="EnsemblBacteria" id="CAI49249">
        <property type="protein sequence ID" value="CAI49249"/>
        <property type="gene ID" value="NP_2316A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701403"/>
    <dbReference type="KEGG" id="nph:NP_2316A"/>
    <dbReference type="eggNOG" id="arCOG01566">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046377_1_1_2"/>
    <dbReference type="OrthoDB" id="350705at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1640.10">
        <property type="entry name" value="inorganic pyrophosphatase (n-terminal core)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051319">
        <property type="entry name" value="Oligoribo/pAp-PDE_c-di-AMP_PDE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47618">
        <property type="entry name" value="BIFUNCTIONAL OLIGORIBONUCLEASE AND PAP PHOSPHATASE NRNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47618:SF1">
        <property type="entry name" value="BIFUNCTIONAL OLIGORIBONUCLEASE AND PAP PHOSPHATASE NRNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="DHH phosphoesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDH" evidence="1">
        <location>
            <begin position="153"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="DHHA1" evidence="2">
        <location>
            <begin position="355"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="482" mass="51139" checksum="94B4F92A4E4CF2CC" modified="2017-05-10" version="1">MDRLVLGCAPGTRQFVEQLAEQPASLLVLVGDEARADTLRDSGIDTRVVDPTDPAAIRTVAGDAASVVVAPEASPEAAAIADAARSAYPDTFLLACFGRQPSEPVRSEVAAVADRVVDGLAETRDKLLAAVGEGGLRTRKLQHVLRTIDGPLAVVAHDNPDPDAIGSAIGLKRIAEAAGVEATACYYGDINHQENRALVNLLEFDLRSLDADCDPSSFGGIALVDHSRPGANDGLPPEADIDIVIDHHPPRQPIQARFVDLRSDVGATCTLVAGYLRELGIPIDENLASGLLYGIWTDTQSFSRGVARADFEAAAALIETADREALQRIESPSVTAGTLETVQRAISNRTVRDDVLVTCVGQVSDRDALSQAADKLLNMQDISTTLVCGYTDETVFISARARAADLDLGETLREAFGQIGSAGGHADMAGAQIPVGILLDDADERERRSVIEDVITERFFEAVGMTPDYVPFVYADTFGGVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW94</accession>
    <name>A0A1U7EW94_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein-export membrane protein SecD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 12" type="primary">secD</name>
        <name evidence="12" type="ordered locus">NP_2522A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in protein export.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Part of the protein translocation apparatus. Forms a complex with SecF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SecD/SecF family. SecD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322978.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW94"/>
    <dbReference type="STRING" id="348780.NP_2522A"/>
    <dbReference type="EnsemblBacteria" id="CAI49352">
        <property type="protein sequence ID" value="CAI49352"/>
        <property type="gene ID" value="NP_2522A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703200"/>
    <dbReference type="KEGG" id="nph:NP_2522A"/>
    <dbReference type="eggNOG" id="arCOG03055">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007894_5_0_2"/>
    <dbReference type="OrthoDB" id="146638at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3400">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1640.10">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01463_A">
        <property type="entry name" value="SecD_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022813">
        <property type="entry name" value="SecD/SecF_arch_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024912">
        <property type="entry name" value="SecD_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048634">
        <property type="entry name" value="SecD_SecF_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006215">
        <property type="entry name" value="PRK08343.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30081:SF1">
        <property type="entry name" value="PROTEIN TRANSLOCASE SUBUNIT SECD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30081">
        <property type="entry name" value="PROTEIN-EXPORT MEMBRANE PROTEIN SEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02355">
        <property type="entry name" value="SecD_SecF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82866">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="405"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="436"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="461"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="502"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="528"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="Protein export membrane protein SecD/SecF C-terminal" evidence="11">
        <location>
            <begin position="397"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="34"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="34"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI49352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="562" mass="60071" checksum="D409242C2664C265" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWA1</accession>
    <name>A0A1U7EWA1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2532A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322982.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWA1"/>
    <dbReference type="EnsemblBacteria" id="CAI49357">
        <property type="protein sequence ID" value="CAI49357"/>
        <property type="gene ID" value="NP_2532A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701477"/>
    <dbReference type="KEGG" id="nph:NP_2532A"/>
    <dbReference type="eggNOG" id="arCOG10817">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080639_0_0_2"/>
    <dbReference type="OrthoDB" id="197051at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="218"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="300" mass="30480" checksum="3B8265BECC5EA9A3" modified="2017-05-10" version="1">MSSGTERDPSRPPAVRRREDLDGCGCLRCRGLDYAVVSRSFGAAFIWGGRLLRRRPSVLLVFVLVGVAQQLSLLVPMEPGIAAAFVGIAGVFFGRGYAGLVGAGDLRGSDARFPPVVAAATRLPAALGAFGVAALGAAAVAVGLVIAGSRAGPALATTLGVGGGRVFDALVLAAAVGVVVYVLVKCCFVPEACFIGGYGPLESIRVSWALTTMHRRKAVALTAGLVALFAVSVALEAGVDAGRPVVISVTFAGTTVPVRSIGLSTATVPRLAADAGLSAVYYLVFAHQYVQGLVDEAAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWE5</accession>
    <name>A0A1U7EWE5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transport protein (Probable substrate zinc)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">tp46</name>
        <name evidence="2" type="ordered locus">NP_2636A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323034.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWE5"/>
    <dbReference type="SMR" id="A0A1U7EWE5"/>
    <dbReference type="STRING" id="348780.NP_2636A"/>
    <dbReference type="EnsemblBacteria" id="CAI49409">
        <property type="protein sequence ID" value="CAI49409"/>
        <property type="gene ID" value="NP_2636A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703324"/>
    <dbReference type="KEGG" id="nph:NP_2636A"/>
    <dbReference type="eggNOG" id="arCOG00576">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_896064_0_0_2"/>
    <dbReference type="OrthoDB" id="11839at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF205">
        <property type="entry name" value="ZINC TRANSPORTER ZUPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="221"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="284"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="307" mass="31904" checksum="6B658146F953C248" modified="2017-05-10" version="1">MDSDSAPLSGLPDWVLAFGPFAIIGVLIGASFAALSALEFDWLAASGTAEILAVMTVLGFVAGILPVVVGMLWFPYVRRLDVVWVHAILAFSAGILAYIAFEMADEALEAGAEVSTSLLAESVILVSAGGTIVLMEAFSRWRRNRSSGSDRAGLRVAYLVAIGLGLHSVGEGLAIGTAFVLGESGLVVLLTIGFVIHNVTEGPAVISAVAHDAETPPLRHFALLGLLAGGGVIVGGWLGSFVDSALVAATCFAIAFGAIVQVIWEMRGLIRADADALITKRTAVAFIVGVIVMFTLEDVIVEAWLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWE6</accession>
    <name>A0A1U7EWE6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">CBS domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cbs5</name>
        <name evidence="4" type="ordered locus">NP_2638A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323035.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWE6"/>
    <dbReference type="STRING" id="348780.NP_2638A"/>
    <dbReference type="EnsemblBacteria" id="CAI49410">
        <property type="protein sequence ID" value="CAI49410"/>
        <property type="gene ID" value="NP_2638A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702583"/>
    <dbReference type="KEGG" id="nph:NP_2638A"/>
    <dbReference type="eggNOG" id="arCOG00606">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040681_12_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="7"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="105"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="167" mass="17949" checksum="0738BC7563FDA9A0" modified="2017-05-10" version="1">MQARDIMTDEVETVAPDDDVGDVLTRLARANFNGFPVVEDGLLVGIVTQGDLVDLFQPSDRTLWIPVGFPPFLESLTYGVDLSWDEFDLGRDMAKNAGRPVSEVMTEDVVTVGPDADLDAVLALLADRDRDINRLPVVDGAGVVLGIIAREDLLAALKAVRDAGDAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWF5</accession>
    <name>A0A1U7EWF5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">NP_2656A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323044.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="STRING" id="348780.NP_2656A"/>
    <dbReference type="EnsemblBacteria" id="CAI49419">
        <property type="protein sequence ID" value="CAI49419"/>
        <property type="gene ID" value="NP_2656A"/>
    </dbReference>
    <dbReference type="GeneID" id="32155004"/>
    <dbReference type="KEGG" id="nph:NP_2656A"/>
    <dbReference type="eggNOG" id="arCOG02354">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02376">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02389">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06408">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06796">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_248708_0_0_2"/>
    <dbReference type="OrthoDB" id="3369at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004673">
        <property type="term" value="F:protein histidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00075">
        <property type="entry name" value="HATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="CDD" id="cd00156">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013656">
        <property type="entry name" value="PAS_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052162">
        <property type="entry name" value="Sensor_kinase/Photoreceptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43304:SF1">
        <property type="entry name" value="PAC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43304">
        <property type="entry name" value="PHYTOCHROME-LIKE PROTEIN CPH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08448">
        <property type="entry name" value="PAS_4"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        </location>
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        </location>
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        </location>
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        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
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        <source>
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        </source>
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        <source>
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    </evidence>
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        </source>
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            <dbReference type="PROSITE" id="PS50110"/>
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        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="1492" mass="170013" checksum="C0F2A1021E7ABE47" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWG1</accession>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
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                <person name="Tittor J."/>
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            </authorList>
            <dbReference type="PubMed" id="16169924"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="388" mass="41814" checksum="4B7282D74E278B54" modified="2017-05-10" version="1">MASTAEQAPVGISATELKRLFDSGERFVLVDTRDRESFESWRLADARQYLYKPHHEFDCEAFEAETGIGSEESVVTICAKGKSSRALAEELAAAGYDDVRYVEDGMRAWSAVYDAVDIDAGGDADIVQIQRRAKGCLGYLVADPEAGVAAAVDPTRHTDEFVAAAAERGWQIERVLDTHIHADHISGGRQLADELSVPYHLGERVSDRDVGVDYEPLARNEVVTVGDVDLKAVFTPGHTSGMASYLVDENALLTGDTVFVDSVGRTELQFGAADADTGAKLLYESLHGTILAEPDSVTVLPGHFHVGDDGSYGIDPGTPAATTVAKLRTELPVLELDRASFVATVRDAAPEKPPNYERIIAINRGRASPDDEVEAIELELGPNRCAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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        </submittedName>
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        <name evidence="3" type="ordered locus">NP_2718A</name>
    </gene>
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        <lineage>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="348780.NP_2718A"/>
    <dbReference type="EnsemblBacteria" id="CAI49450">
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        <property type="gene ID" value="NP_2718A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_2718A"/>
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    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02109">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR43032:SF4">
        <property type="entry name" value="OXIDOREDUCTASE MOLYBDOPTERIN-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Oxidored_molyb"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Oxidoreductase molybdopterin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidoreductase molybdopterin-binding" evidence="2">
        <location>
            <begin position="33"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="195" mass="21797" checksum="704360AC8A7A40F5" modified="2017-05-10" version="1">MRDVTDLYESFGEDRLPPGQRETSEFPVLSKGETPSVPDDWTLEVWGAVDEPCSLSAAELADLGAETQRQDFHCVTGWSRLGCVFTGVPFPTLADHAGVQPSATHVMFHAHDGYTTDLPLSACDRREVLFAWELDGEPLPADHGGPLRVVVPHRYAYKGAKWVSGVEFLTEPERGYWEKRGYSQTANPWNEERYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWI6</accession>
    <name>A0A1U7EWI6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2720A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323076.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWI6"/>
    <dbReference type="STRING" id="348780.NP_2720A"/>
    <dbReference type="EnsemblBacteria" id="CAI49451">
        <property type="protein sequence ID" value="CAI49451"/>
        <property type="gene ID" value="NP_2720A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701536"/>
    <dbReference type="KEGG" id="nph:NP_2720A"/>
    <dbReference type="eggNOG" id="arCOG04610">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2713016_0_0_2"/>
    <dbReference type="OrthoDB" id="303497at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22231">
        <property type="entry name" value="RHH_NikR_HicB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055544">
        <property type="entry name" value="DUF7120"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23434">
        <property type="entry name" value="DUF7120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="Ribbon-helix-helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="70" mass="7955" checksum="D1E3F7957907D597" modified="2017-05-10" version="1">MPKVQLTIPEHLEMQIAQLVEEGEFVNREEAIEELLSTGLRAYKTSGPMDDEDPGLEEDGMMGHDDEYVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWJ3</accession>
    <name>A0A1U7EWJ3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">O-succinylbenzoate--CoA ligase</fullName>
            <ecNumber evidence="7">6.2.1.26</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">menE</name>
        <name evidence="7" type="synonym">acs12</name>
        <name evidence="7" type="ordered locus">NP_2738A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.26" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323085.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWJ3"/>
    <dbReference type="STRING" id="348780.NP_2738A"/>
    <dbReference type="EnsemblBacteria" id="CAI49460">
        <property type="protein sequence ID" value="CAI49460"/>
        <property type="gene ID" value="NP_2738A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702911"/>
    <dbReference type="KEGG" id="nph:NP_2738A"/>
    <dbReference type="eggNOG" id="arCOG00856">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_0_2"/>
    <dbReference type="OrthoDB" id="35688at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008756">
        <property type="term" value="F:o-succinylbenzoate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009234">
        <property type="term" value="P:menaquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050237">
        <property type="entry name" value="ATP-dep_AMP-bd_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010192">
        <property type="entry name" value="MenE"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01923">
        <property type="entry name" value="menE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43767">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43767:SF1">
        <property type="entry name" value="NONRIBOSOMAL PEPTIDE SYNTHASE PES1 (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0474">Menaquinone biosynthesis</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="5">
        <location>
            <begin position="7"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="6">
        <location>
            <begin position="400"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="488" mass="51212" checksum="0420BB0B947A7E46" modified="2017-05-10" version="1">MRDWLAVRADATPGATALAVADGPDRSYDELDERVEVLAGRLAALGVGVGDTVAVCLETRHEFVVVVHALQRLGAVLVPLHTEQTPPELRTRLSAVPPEAAVCGAATEAALTDAAETTVVTVDEPTADARPLSGVTPEPFDLPEWALDEPLAVLFTSGTTGEPKGVVLTLGNVLASATASAFRLGVQRGDCWHVPLPMYHMGGLAPVYRSVLYGTALSVQSTFDAEATAERLRNVEATAVSLVPTMLERLIAVGDLPDLRFALVGGAPCPASLVERAHDRGVPVAPTYGMTEAASQIATAAPSMARAHPHSVGYPLMFAEVTVVNDEGVGCEPGTVGDIVVDGPMVTPGYLDDETTAARFTARGLRTGDRGYRDDDGRLHVVGRSDDTIVTGGENVDPTEVEAALRAHSAVDACAVVGLADDEWGQRVAALVVSDGDDTTPDALREHCRERLAGYKLPRTIGVVDALPRTASGTVDRDAARDRLSECQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWJ5</accession>
    <name>A0A1U7EWJ5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DUF123 domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2746A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWJ5"/>
    <dbReference type="STRING" id="348780.NP_2746A"/>
    <dbReference type="EnsemblBacteria" id="CAI49464">
        <property type="protein sequence ID" value="CAI49464"/>
        <property type="gene ID" value="NP_2746A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_2746A"/>
    <dbReference type="eggNOG" id="arCOG00463">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115699_0_0_2"/>
    <dbReference type="OrthoDB" id="17296at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd10441">
        <property type="entry name" value="GIY-YIG_COG1833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002837">
        <property type="entry name" value="DUF123"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37460">
        <property type="entry name" value="ENDONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37460:SF1">
        <property type="entry name" value="ENDONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01986">
        <property type="entry name" value="DUF123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="135" mass="14360" checksum="14D2E2F07A977450" modified="2017-05-10" version="1">MSGGTYTLILELESPVTVEIGALGTHRLPAGQYAYTGSALGTGGFSRVDRHYELAAGDRSVRHWHIDYLLGHEAVSLRGDVRTPDADIECAVAADLPDGPVDGFGASDCNCRSHLSAGDDIRDRVAAAHRRATIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWU6</accession>
    <name>A0A1U7EWU6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF2249 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2948A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323189.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWU6"/>
    <dbReference type="STRING" id="348780.NP_2948A"/>
    <dbReference type="EnsemblBacteria" id="CAI49565">
        <property type="protein sequence ID" value="CAI49565"/>
        <property type="gene ID" value="NP_2948A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701608"/>
    <dbReference type="KEGG" id="nph:NP_2948A"/>
    <dbReference type="eggNOG" id="arCOG03929">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_157868_1_0_2"/>
    <dbReference type="OrthoDB" id="281801at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018720">
        <property type="entry name" value="DUF2249"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10006">
        <property type="entry name" value="DUF2249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2249" evidence="1">
        <location>
            <begin position="10"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="75" mass="8348" checksum="841209DD1FD2F3E0" modified="2017-05-10" version="1">MAPDTAAETVLDVRDIEGEPFDDIMAALTELGADETLLLINSFEPKPLYGVLEERGFIYDTSQTDGTWRIEIEQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWW9</accession>
    <name>A0A1U7EWW9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3006A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49594.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323218.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWW9"/>
    <dbReference type="STRING" id="348780.NP_3006A"/>
    <dbReference type="EnsemblBacteria" id="CAI49594">
        <property type="protein sequence ID" value="CAI49594"/>
        <property type="gene ID" value="NP_3006A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701624"/>
    <dbReference type="KEGG" id="nph:NP_3006A"/>
    <dbReference type="eggNOG" id="arCOG15171">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2911624_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49594.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="61" mass="6417" checksum="2C865F61ADD0C95C" modified="2017-05-10" version="1">MSSLVQDDSTRAATFFAAAFLLTALGGLLIGEFRVGVQWGLGLGAAFAVFAYFFITPAPDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWY1</accession>
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        <recommendedName>
            <fullName evidence="2">peptidylprolyl isomerase</fullName>
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        </recommendedName>
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        <name evidence="8" type="primary">ppiA</name>
        <name evidence="8" type="ordered locus">NP_3056A</name>
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        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <lineage>
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            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
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            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWY1"/>
    <dbReference type="STRING" id="348780.NP_3056A"/>
    <dbReference type="EnsemblBacteria" id="CAI49619">
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        <property type="gene ID" value="NP_3056A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703045"/>
    <dbReference type="KEGG" id="nph:NP_3056A"/>
    <dbReference type="eggNOG" id="arCOG04767">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_16_3_2"/>
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    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="cyclophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000028">
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625:SF4">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE DOMAIN AND WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001467">
        <property type="entry name" value="Peptidylpro_ismrse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="7">
        <location>
            <begin position="22"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="167"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="178"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="191" mass="20564" checksum="969C217337785C27" modified="2017-05-10" version="1">MSDDASVSDPENPTATIATTHGDITVELFEQRAPRTVENFIGLATGANDYEGTDIEPGTGAWEDPETGEKRIDPLYDDVPFHRVIEDFMIQGGDPTGTGRGGPGYQFDDEFHDELSHDGPGILSMANSGPDTNGSQFFITLDAQPHLDGRHAVFGQVIDGMDVVEEVGSLPTGRNDDPQEDVSIESVDVDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX00</accession>
    <name>A0A1U7EX00_NATPD</name>
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        <submittedName>
            <fullName evidence="9">Transducer protein Htr23</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">htr23</name>
        <name evidence="9" type="ordered locus">NP_3122A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
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        <property type="protein sequence ID" value="CAI49652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX00"/>
    <dbReference type="STRING" id="348780.NP_3122A"/>
    <dbReference type="EnsemblBacteria" id="CAI49652">
        <property type="protein sequence ID" value="CAI49652"/>
        <property type="gene ID" value="NP_3122A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702838"/>
    <dbReference type="KEGG" id="nph:NP_3122A"/>
    <dbReference type="eggNOG" id="arCOG02320">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_18_2"/>
    <dbReference type="OrthoDB" id="8523at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06225">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.950">
        <property type="entry name" value="Methyl-accepting chemotaxis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004090">
        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
    </dbReference>
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        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32089:SF112">
        <property type="entry name" value="LYSOZYME-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32089">
        <property type="entry name" value="METHYL-ACCEPTING CHEMOTAXIS PROTEIN MCPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00260">
        <property type="entry name" value="CHEMTRNSDUCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158472">
        <property type="entry name" value="HAMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58104">
        <property type="entry name" value="Methyl-accepting chemotaxis protein (MCP) signaling domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0807">Transducer</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="15"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="45"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="8">
        <location>
            <begin position="70"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="8">
        <location>
            <begin position="143"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="7">
        <location>
            <begin position="215"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="506"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="117"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="205"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="293"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="518"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="563"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI49652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="602" mass="61690" checksum="D4FB656398A34779" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX40</accession>
    <name>A0A1U7EX40_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATP phosphoribosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3148A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323287.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX40"/>
    <dbReference type="STRING" id="348780.NP_3148A"/>
    <dbReference type="EnsemblBacteria" id="CAI49665">
        <property type="protein sequence ID" value="CAI49665"/>
        <property type="gene ID" value="NP_3148A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701669"/>
    <dbReference type="KEGG" id="nph:NP_3148A"/>
    <dbReference type="eggNOG" id="arCOG04572">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_932567_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058892">
        <property type="entry name" value="HisG-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25936">
        <property type="entry name" value="HisG_halo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="289" mass="31952" checksum="E684BD3710CFD06D" modified="2017-05-10" version="1">MTTRVAVPRKGRPLEAVLERVASLAGVEDRADAIASTLRYEKAVTKGNQTDERSVYDRLADYSDTDTYAPEYTLFRDARPGMPRRIVFDSWRVPVEDGELHLVGREEPFRALRKHEFALGFDAADLVLEEVVGIEPEPLGSLSELNDRIDPRDTDVRVAGGLGDTVYHTLLATPDVSESPTREFIEDYEGPLCISPRYERLVEAVLGTDAMEGVSFRYPGEREEEAAIASVGMGVYLTVTGSTARDHGLRVGEQLFPSETVLLENPPERSADTDALLEQLVGSRLTTPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX67</accession>
    <name>A0A1U7EX67_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Anthranilate synthase component 1</fullName>
            <ecNumber evidence="11">4.1.3.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">trpE1</name>
        <name evidence="11" type="synonym">trpE</name>
        <name evidence="14" type="ordered locus">NP_3342A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21732"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="4.1.3.27"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE).</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the anthranilate synthase component I family.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.27" evidence="11"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323382.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX67"/>
    <dbReference type="STRING" id="348780.NP_3342A"/>
    <dbReference type="EnsemblBacteria" id="CAI49762">
        <property type="protein sequence ID" value="CAI49762"/>
        <property type="gene ID" value="NP_3342A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703414"/>
    <dbReference type="KEGG" id="nph:NP_3342A"/>
    <dbReference type="eggNOG" id="arCOG02014">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006493_9_0_2"/>
    <dbReference type="OrthoDB" id="25514at2157"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00040"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004049">
        <property type="term" value="F:anthranilate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.120.10">
        <property type="entry name" value="Anthranilate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005801">
        <property type="entry name" value="ADC_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019999">
        <property type="entry name" value="Anth_synth_I-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006805">
        <property type="entry name" value="Anth_synth_I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010116">
        <property type="entry name" value="Anthranilate_synth_I_arc_typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015890">
        <property type="entry name" value="Chorismate_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01820">
        <property type="entry name" value="TrpE-arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236">
        <property type="entry name" value="AMINOBENZOATE/ANTHRANILATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236:SF9">
        <property type="entry name" value="ANTHRANILATE SYNTHASE COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04715">
        <property type="entry name" value="Anth_synt_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00425">
        <property type="entry name" value="Chorismate_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00095">
        <property type="entry name" value="ANTSNTHASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56322">
        <property type="entry name" value="ADC synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8 11" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="9 11 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Anthranilate synthase component I N-terminal" evidence="13">
        <location>
            <begin position="30"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Chorismate-utilising enzyme C-terminal" evidence="12">
        <location>
            <begin position="249"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAI49762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="531" mass="57464" checksum="9F131B413434C8B7" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX94</accession>
    <name>A0A1U7EX94_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable secreted glycoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3376A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323399.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX94"/>
    <dbReference type="STRING" id="348780.NP_3376A"/>
    <dbReference type="EnsemblBacteria" id="CAI49779">
        <property type="protein sequence ID" value="CAI49779"/>
        <property type="gene ID" value="NP_3376A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701755"/>
    <dbReference type="KEGG" id="nph:NP_3376A"/>
    <dbReference type="eggNOG" id="arCOG02087">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG03256">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_266656_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1120">
        <property type="entry name" value="Carboxypeptidase-like, regulatory domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013784">
        <property type="entry name" value="Carb-bd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008965">
        <property type="entry name" value="CBM2/CBM3_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13620">
        <property type="entry name" value="CarboxypepD_reg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49384">
        <property type="entry name" value="Carbohydrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49452">
        <property type="entry name" value="Starch-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="582"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1056"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1121"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="586"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1056"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1131"/>
            <end position="1140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="1184" mass="124380" checksum="F3F601A895B9B181" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX99</accession>
    <name>A0A1U7EX99_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Dolichyl-phosphate hexosyltransferase AglJ</fullName>
            <ecNumber evidence="3">2.4.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">aglJ2</name>
        <name evidence="3" type="synonym">gtl6</name>
        <name evidence="3" type="ordered locus">NP_3366A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.4.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49774.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX99"/>
    <dbReference type="STRING" id="348780.NP_3366A"/>
    <dbReference type="EnsemblBacteria" id="CAI49774">
        <property type="protein sequence ID" value="CAI49774"/>
        <property type="gene ID" value="NP_3366A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3366A"/>
    <dbReference type="eggNOG" id="arCOG00894">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033536_7_3_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04179">
        <property type="entry name" value="DPM_DPG-synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026456">
        <property type="entry name" value="GCTrfase_AglJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050256">
        <property type="entry name" value="Glycosyltransferase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04182">
        <property type="entry name" value="glyco_TIGR04182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48090:SF7">
        <property type="entry name" value="RFBJ PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48090">
        <property type="entry name" value="UNDECAPRENYL-PHOSPHATE 4-DEOXY-4-FORMAMIDO-L-ARABINOSE TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="229"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="2">
        <location>
            <begin position="8"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49774.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="312" mass="33885" checksum="C32DDAED80B34504" modified="2017-05-10" version="1">MPDRTDVCVLLPTLNESETIGDVVGGFYDHGFEHVLVVDGDSTDGTRAIAREHGARVITQSGSGKGQAVREGVEQIDQPYVLMADGDGTYRPEDADKMLTPLFAGRAEHVIGDRFADMEAGAMTRLNAVGNRLINRLFATIHGRDLQDILSGYRAFTTQSFERSKLSADGFGIETELAVECVRRGVSTAVVPIRYEARPGGSETNLHPLKDGGRILLTLYRLARMNNPIFYFGSVAAVGTAGGVALGAFVGYRYLTAGVSHEVLALLAAFLVLLSVQLLMFGVLSDIIVSIHREQTRRLDDIADQLEEQNDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXB2</accession>
    <name>A0A1U7EXB2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC-type transport system periplasmic substrate-binding protein (Probable substrate branched-chain amino acids)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">livJ4</name>
        <name evidence="3" type="synonym">abc08s</name>
        <name evidence="3" type="ordered locus">NP_3424A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323423.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXB2"/>
    <dbReference type="STRING" id="348780.NP_3424A"/>
    <dbReference type="EnsemblBacteria" id="CAI49803">
        <property type="protein sequence ID" value="CAI49803"/>
        <property type="gene ID" value="NP_3424A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702391"/>
    <dbReference type="KEGG" id="nph:NP_3424A"/>
    <dbReference type="eggNOG" id="arCOG01024">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027128_1_4_2"/>
    <dbReference type="OrthoDB" id="264684at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06330">
        <property type="entry name" value="PBP1_As_SBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051010">
        <property type="entry name" value="BCAA_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028081">
        <property type="entry name" value="Leu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30483:SF37">
        <property type="entry name" value="ABC TRANSPORTER SUBSTRATE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30483">
        <property type="entry name" value="LEUCINE-SPECIFIC-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13458">
        <property type="entry name" value="Peripla_BP_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Leucine-binding protein" evidence="2">
        <location>
            <begin position="50"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="447" mass="48973" checksum="4D701DC48173F4AF" modified="2017-05-10" version="1">MTDNRIRSSRRRLLKGAAAAGGIGAFAGCALLEEAGEEEQLDGIPDEPFRIVHFTFESGPPAFYGDEMINAVDMMVDRINDRGGLLGEREIEIVDRIDEDAGVESMRGSVRQIAQNDEADMLMGLISSANALSVVPAAEENQIPFLVTVPGTYQLFEENPDLEYAVRTAGTNAAGAIGGARIVEQMDDVETVVTVDQDYAWGHDHRDMFVEALERIRPDIEVLETRTPEPFISDYSAHISAVQDLEPDVVFSSLWGGDISTFTSQAVDDGLFDDIGTGVFVGDTGAPLLRALGDDMPENVIMGGRGGYQPNYRYETAEDHQEFVDDYFDRYGDMPGWGAYHDWVAFQALEAGVEQAVQLTGNWPTGSQLMSSIRGLSFETTHGSHVMAQAGGRQASAPGVFGQPTPDHDFPVDHHLLTDYEFIPAHECNPPEGMDTFEWVEQIEPVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXF8</accession>
    <name>A0A1U7EXF8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uridine phosphorylase</fullName>
            <ecNumber evidence="5">2.4.2.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">udp1</name>
        <name evidence="5" type="ordered locus">NP_3510A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PNP/UDP phosphorylase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.3" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323466.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXF8"/>
    <dbReference type="STRING" id="348780.NP_3510A"/>
    <dbReference type="EnsemblBacteria" id="CAI49846">
        <property type="protein sequence ID" value="CAI49846"/>
        <property type="gene ID" value="NP_3510A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703440"/>
    <dbReference type="KEGG" id="nph:NP_3510A"/>
    <dbReference type="eggNOG" id="arCOG01324">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068457_0_0_2"/>
    <dbReference type="OrthoDB" id="372263at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004850">
        <property type="term" value="F:uridine phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009164">
        <property type="term" value="P:nucleoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17767">
        <property type="entry name" value="UP_EcUdp-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018016">
        <property type="entry name" value="Nucleoside_phosphorylase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43691">
        <property type="entry name" value="URIDINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43691:SF13">
        <property type="entry name" value="URIDINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01232">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside phosphorylase" evidence="4">
        <location>
            <begin position="19"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="267" mass="28148" checksum="C51D297A83E08992" modified="2017-05-10" version="1">MSDDQYHIELSDGDVDGPVLLPGDPDRIDIIADCWDNAEPLADHREYRTAAGEYDGEPLAATSTGIGSPSAAIAVEELARVGAETFIRVGSCGTIQPEVEVGDLVITTAAVRKEGTSDEYIRADYPASAHDEVVTALVTAAERLGHEYHLGVTCSTDSFYAGQARDGVGGFRAAGAEAELEALREAGVLNFEMEASTVLTLAGLYGLRAGAVCTVYADRTTGEFRTDGQQKAAETASLAAALLSKMDRLKNAAGVDRWHAGLSLDDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXQ7</accession>
    <name>A0A1U7EXQ7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">dihydropteroate synthase</fullName>
            <ecNumber evidence="4">2.5.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">folP2</name>
        <name evidence="10" type="ordered locus">NP_3770A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(7,8-dihydropterin-6-yl)methyl diphosphate + 4-aminobenzoate = 7,8-dihydropteroate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19949"/>
            <dbReference type="ChEBI" id="CHEBI:17836"/>
            <dbReference type="ChEBI" id="CHEBI:17839"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:72950"/>
            <dbReference type="EC" id="2.5.1.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate: step 1/2.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.15" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323593.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXQ7"/>
    <dbReference type="STRING" id="348780.NP_3770A"/>
    <dbReference type="EnsemblBacteria" id="CAI49976">
        <property type="protein sequence ID" value="CAI49976"/>
        <property type="gene ID" value="NP_3770A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702725"/>
    <dbReference type="KEGG" id="nph:NP_3770A"/>
    <dbReference type="eggNOG" id="arCOG01978">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008023_3_0_2"/>
    <dbReference type="OrthoDB" id="371861at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004156">
        <property type="term" value="F:dihydropteroate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="entry name" value="Dihydropteroate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045031">
        <property type="entry name" value="DHP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006390">
        <property type="entry name" value="DHP_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000489">
        <property type="entry name" value="Pterin-binding_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01496">
        <property type="entry name" value="DHPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20941">
        <property type="entry name" value="FOLATE SYNTHESIS PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20941:SF1">
        <property type="entry name" value="FOLIC ACID SYNTHESIS PROTEIN FOL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00809">
        <property type="entry name" value="Pterin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="Dihydropteroate synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50972">
        <property type="entry name" value="PTERIN_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pterin-binding" evidence="9">
        <location>
            <begin position="17"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI49976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="378" mass="40898" checksum="BABBF849A6E310AE" modified="2017-05-10" version="1">MRTVDAAGLQIGDDHPPRIMGVLNVSEESPYDPSVFDEPDEAARYVDEDLIGEGADIVDVGLESANKKFEVLSADQELDRLDTAVETLRSTSGDAVWSIETRYHEVAEAALDAGFDMVNDICGFADPEMTTVCAEYDVAVSKMASPPDLERPGAIDDVDDIYAALEQNGFTEKTILDPAFGGWSETKTIEDDRETFRRLREFRAYGRPLLVSINRKNFLRDIAGRDTEAALPVSLAATSMAVERGAHVIRTHDVAETRDAALVGHEFTPERANGSGFEALDVTTDRELARHLGRTVDDAPEPSVLPHAVAFGLPSGPAAYLGEQARTVGLETYHGDRLVVVGTADGFKTLRTAIEDDADAPQRASEAITELSAQFGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXT0</accession>
    <name>A0A1U7EXT0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">HTH domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3804A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49993.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939633.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXT0"/>
    <dbReference type="EnsemblBacteria" id="CAI49993">
        <property type="protein sequence ID" value="CAI49993"/>
        <property type="gene ID" value="NP_3804A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701870"/>
    <dbReference type="KEGG" id="nph:NP_3804A"/>
    <dbReference type="eggNOG" id="arCOG02808">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071220_0_1_2"/>
    <dbReference type="OrthoDB" id="330490at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013561">
        <property type="entry name" value="FilR1_middle_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057527">
        <property type="entry name" value="HVO_A0261-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08350">
        <property type="entry name" value="FilR1_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25213">
        <property type="entry name" value="HVO_A0261_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HVO-A0261-like N-terminal" evidence="2">
        <location>
            <begin position="7"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Methanogenesis regulatory protein FilR1 middle" evidence="1">
        <location>
            <begin position="122"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49993.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="262" mass="28720" checksum="05B63B30CEC58AA9" modified="2017-05-10" version="1">MDDALAEMEFLARSGNRIAVLEAVSERPHTRSELAERTGASQATLGRILRDFEERNWLARTDGAYVATPTGNLVADGVLDLRKRLETERRLRPVVGMLPDELDVDLRRLRTATLTTPTETRPSAPVTRSLDILREAGHIRAVSRALNDRTLRLLRRRAVADGCTVDGVFAARAIEALVDDDRLRPKLRELVAAESADIHVYEGPLSLAVTLADGTVSLLLRDDDGVVQAALDTDDEAVAEWAASVHEQHRTAAEPLSPADIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY18</accession>
    <name>A0A1U7EY18_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Deoxyribodipyrimidine photolyase</fullName>
            <ecNumber evidence="10">4.1.99.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">phr2</name>
        <name evidence="10" type="ordered locus">NP_4042A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="5">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DNA photolyase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.3" evidence="10"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323728.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY18"/>
    <dbReference type="STRING" id="348780.NP_4042A"/>
    <dbReference type="EnsemblBacteria" id="CAI50112">
        <property type="protein sequence ID" value="CAI50112"/>
        <property type="gene ID" value="NP_4042A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703031"/>
    <dbReference type="KEGG" id="nph:NP_4042A"/>
    <dbReference type="eggNOG" id="arCOG02840">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010348_2_0_2"/>
    <dbReference type="OrthoDB" id="11721at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003904">
        <property type="term" value="F:deoxyribodipyrimidine photo-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.579.10:FF:000003">
        <property type="entry name" value="Deoxyribodipyrimidine photo-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.579.10">
        <property type="entry name" value="DNA Cyclobutane Dipyrimidine Photolyase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036134">
        <property type="entry name" value="Crypto/Photolyase_FAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036155">
        <property type="entry name" value="Crypto/Photolyase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005101">
        <property type="entry name" value="Cryptochr/Photolyase_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002081">
        <property type="entry name" value="Cryptochrome/DNA_photolyase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018394">
        <property type="entry name" value="DNA_photolyase_1_CS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006050">
        <property type="entry name" value="DNA_photolyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11455">
        <property type="entry name" value="CRYPTOCHROME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11455:SF9">
        <property type="entry name" value="CRYPTOCHROME CIRCADIAN CLOCK 5 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00875">
        <property type="entry name" value="DNA_photolyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03441">
        <property type="entry name" value="FAD_binding_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00147">
        <property type="entry name" value="DNAPHOTLYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48173">
        <property type="entry name" value="Cryptochrome/photolyase FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52425">
        <property type="entry name" value="Cryptochrome/photolyase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00394">
        <property type="entry name" value="DNA_PHOTOLYASES_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00691">
        <property type="entry name" value="DNA_PHOTOLYASES_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51645">
        <property type="entry name" value="PHR_CRY_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0157">Chromophore</keyword>
    <keyword evidence="3 5 7" id="KW-0274">FAD</keyword>
    <keyword evidence="2 5 7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Photolyase/cryptochrome alpha/beta" evidence="9">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="429"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="221"/>
            <end position="225"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="264"/>
            <end position="271"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="361"/>
            <end position="363"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="site" description="Electron transfer via tryptophanyl radical" evidence="6">
        <location>
            <position position="295"/>
        </location>
    </feature>
    <feature type="site" description="Electron transfer via tryptophanyl radical" evidence="6">
        <location>
            <position position="348"/>
        </location>
    </feature>
    <feature type="site" description="Electron transfer via tryptophanyl radical" evidence="6">
        <location>
            <position position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602081-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602081-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI50112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="462" mass="52447" checksum="B2D49983DA13D200" modified="2017-05-10" version="1">MRIHWHRRDLRVADNRGLADAPGRPVGLFVFDEAVLDHAAPPRVAFLLDALSSLRAAYRDRGSELFIRRGDPANVLSALADDLGVDVVSWNRDYSGLAADRDDRVADVLDEVGVTPQRFHDAICHPPGTITTNAGDPYSVYSYFWKKWRDREKDDPYPVPALADATDETTLPTLADLGFDEPTAELPKAGTAPARGRLAAFLEDDIYRYEEARDYPATECTSRLSADLKFGTIGIRAVDKQVREAIAAAETDSERESAAEFRSQLAWREFYTHVLAFNPEVVTENYKRYEHDIDWEDDPELLAAWKRGETGYPIVDAGMRQLQAEAYMHNRVRMIVASFLTKDLLIDWRHGYAHFRDRLVDHDTANDNGGWQWAASTGTDAQPYFRIFNPMTQGERYDPDAEYIREYVPELSGVEADVIHSWHELSPTQRANAAPEYPQPVVDHSQRREQALDMFEQARGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY63</accession>
    <name>A0A1U7EY63_NATPD</name>
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        <submittedName>
            <fullName evidence="4">DUF2110 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_4104A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY63"/>
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    <dbReference type="EnsemblBacteria" id="CAI50143">
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        <property type="gene ID" value="NP_4104A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701973"/>
    <dbReference type="KEGG" id="nph:NP_4104A"/>
    <dbReference type="eggNOG" id="arCOG04612">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082927_0_0_2"/>
    <dbReference type="OrthoDB" id="120309at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056757">
        <property type="entry name" value="DUF2110_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056756">
        <property type="entry name" value="DUF2110_central"/>
    </dbReference>
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        <property type="entry name" value="DUF2110_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016757">
        <property type="entry name" value="UCP019322"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09883">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UCP019322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2110" evidence="2">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2110" evidence="1">
        <location>
            <begin position="75"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2110" evidence="3">
        <location>
            <begin position="156"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09883"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF24872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="223" mass="24204" checksum="7F92F38528B409DE" modified="2017-05-10" version="1">MVVLATKLYVSGESQRRAMDSLESLVSNVLDGLDVEFTLGLRDDDFPSVTVTGEDAPVARNLLEEEWGAIVPHREPGGVYVGTLESWDEDGLVLDAGEAVRIPGDELGLGQGSPDQIRKRFGLVQHMPLRFVEGGDDPARLADAERDRLYDWTRGNGRVNANSTTRAQLRATVNRAGHAQDIVTVERLGLLECSVVCREDTDAPGLLADIGPYLEAELLAVVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY83</accession>
    <name>A0A1U7EY83_NATPD</name>
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        <recommendedName>
            <fullName evidence="3">SepF protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="1" type="ordered locus">NP_4202A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="gene ID" value="NP_4202A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702002"/>
    <dbReference type="KEGG" id="nph:NP_4202A"/>
    <dbReference type="eggNOG" id="arCOG04568">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_177453_0_0_2"/>
    <dbReference type="OrthoDB" id="311695at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043931">
        <property type="entry name" value="DUF5779"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19091">
        <property type="entry name" value="DUF5779"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="96" mass="10440" checksum="C220AC6DBE43177F" modified="2017-05-10" version="1">MGDSDLDLQTAEDELDDLDTGGDVVLGVLDGTTDPDEWVAHVENDDVLVLNIEGDLSELAAGFARDIRELGGELMHFRDFLVISPPGRDIDTDRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY97</accession>
    <name>A0A1U7EY97_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF63 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4190A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323802.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY97"/>
    <dbReference type="STRING" id="348780.NP_4190A"/>
    <dbReference type="EnsemblBacteria" id="CAI50186">
        <property type="protein sequence ID" value="CAI50186"/>
        <property type="gene ID" value="NP_4190A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701999"/>
    <dbReference type="KEGG" id="nph:NP_4190A"/>
    <dbReference type="eggNOG" id="arCOG02177">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_718876_0_0_2"/>
    <dbReference type="OrthoDB" id="84937at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002749">
        <property type="entry name" value="DUF63"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40700:SF1">
        <property type="entry name" value="DUF63 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40700">
        <property type="entry name" value="HYPOTHETICAL MEMBRANE PROTEIN, CONSERVED, DUF63 FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01889">
        <property type="entry name" value="DUF63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="118"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="225"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="319"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="349"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="375" mass="40375" checksum="AE0BB767AAE68B44" modified="2017-05-10" version="1">MDSSDFDLARMWLAVFVGGIVAIVAAAAAFPRRVYNGFLWQYFWGPVDADAHGAVCAVRSGGTTERLYSRAACSGAEGFVAEPGYTTVSTVSYAVVLVFMLIGVLFLLRRLDIEMSPAFYFALFPYMLLGGALRTVEDVNVAFRGEAVGMLIPYPAVALIISPFIYFVMFGFTLAALVGGVWLEGRGTLSRSETFLGGIGTAAVVAVVGWLLYVAGTTDIVEFYLLVPTIVLGGATLTTLVFWWVTERFVPGINEGTGAMGALVVWGHAVDGVANVLSLDWGEQLVGVPYGSKHVINEATVRITGAIQPQWLTDAVGSAWPFFFIKVIAAVVVVWVFNEEIFEESPRYAYLLFIAVLAVGLGPGTRDMLRATLAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYB1</accession>
    <name>A0A1U7EYB1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent dethiobiotin synthetase BioD</fullName>
            <ecNumber evidence="1">6.3.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DTB synthetase</fullName>
            <shortName evidence="1">DTBS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Dethiobiotin synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1 2" type="primary">bioD</name>
        <name evidence="2" type="ordered locus">NP_4230A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA, also called 7,8-diammoniononanoate) to form a ureido ring.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(7R,8S)-7,8-diammoniononanoate + CO2 + ATP = (4R,5S)-dethiobiotin + ADP + phosphate + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15805"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:149469"/>
            <dbReference type="ChEBI" id="CHEBI:149473"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the dethiobiotin synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.3" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323822.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYB1"/>
    <dbReference type="STRING" id="348780.NP_4230A"/>
    <dbReference type="EnsemblBacteria" id="CAI50206">
        <property type="protein sequence ID" value="CAI50206"/>
        <property type="gene ID" value="NP_4230A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702552"/>
    <dbReference type="KEGG" id="nph:NP_4230A"/>
    <dbReference type="eggNOG" id="arCOG00100">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072551_3_1_2"/>
    <dbReference type="OrthoDB" id="201569at2157"/>
    <dbReference type="UniPathway" id="UPA00078">
        <property type="reaction ID" value="UER00161"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004141">
        <property type="term" value="F:dethiobiotin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03109">
        <property type="entry name" value="DTBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00336">
        <property type="entry name" value="BioD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004472">
        <property type="entry name" value="DTB_synth_BioD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00347">
        <property type="entry name" value="bioD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43210">
        <property type="entry name" value="DETHIOBIOTIN SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43210:SF5">
        <property type="entry name" value="DETHIOBIOTIN SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13500">
        <property type="entry name" value="AAA_26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006755">
        <property type="entry name" value="DTB_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0093">Biotin biosynthesis</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="1">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="11"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="106"/>
            <end position="109"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="168"/>
            <end position="169"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="223" mass="23439" checksum="657E4D4C863B187B" modified="2017-05-10" version="1">MTIAVVGTDTGVGKTVVTAGLVAWLREQGRKARAVKPVQTGYPEDDDADYVRNICGEAAAATRLRTFEKPLAPAVAARRADSTIAFDDVVAATRTALAEADIGVVEGAGGLRVPLATDGDQEIVDLVAALDVPALVVARSGLGTLNHTALTVETLRRRSVPVAGVALNRYTAASVAERTNPDELERMCDCPVETLPERPSPAPTAVRQLGEAVATMPNMNEIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYD5</accession>
    <name>A0A1U7EYD5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">RimK family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">NP_4274A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50228.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939665.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYD5"/>
    <dbReference type="STRING" id="348780.NP_4274A"/>
    <dbReference type="EnsemblBacteria" id="CAI50228">
        <property type="protein sequence ID" value="CAI50228"/>
        <property type="gene ID" value="NP_4274A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702016"/>
    <dbReference type="KEGG" id="nph:NP_4274A"/>
    <dbReference type="eggNOG" id="arCOG01589">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_609183_0_0_2"/>
    <dbReference type="OrthoDB" id="200216at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008503">
        <property type="entry name" value="Asp_endopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013651">
        <property type="entry name" value="ATP-grasp_RimK-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004666">
        <property type="entry name" value="Rp_bS6_RimK/Lys_biosynth_LsyX"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00768">
        <property type="entry name" value="rimK_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21621:SF0">
        <property type="entry name" value="BETA-CITRYLGLUTAMATE SYNTHASE B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21621">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 MODIFICATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08443">
        <property type="entry name" value="RimK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05618">
        <property type="entry name" value="Zn_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="5">
        <location>
            <begin position="103"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI50228.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="449" mass="48330" checksum="70582AF719631615" modified="2017-05-10" version="1">MSDVLSVGVLSLHSSKESKAILNAVDDLGYDTEWLRTENTAVDITDGSVTLEPDVDIVVNRLLLSKEEQPAEALGLATMLERARPMLNTPTATMTAMHKFATGTALAEADIPVPDAYMALSSDLLNERRGQFGPEVVYKTAIGTHGGGTWKLDTESTVNPMVGSRQAFLQELIEHDEHRHHDLRVYVVGERIVGAMNRYAPDGEWRTNVALGGSVEDATDELPDEAVSMAKRAADEVGLDYAGVDIVQGEDGYYVLEVNPTAGFRGLYKATGRSPAPHIARLAIERAGGDVDAERVAELSATLDDSRPACMPPKRQPEPVDPPVIGYTEEVVVMGTSGQETVTAKSDTGATRTSIDARLAADIGTGPIKDIVKIKSGSLKSGKSRPVVDVVVGIGGKQHTVSASVEDRSHMDYPLLLGRDILEHYHVDVRKRIDDGERSAPDGTTLEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYE1</accession>
    <name>A0A1U7EYE1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_4304A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323859.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYE1"/>
    <dbReference type="STRING" id="348780.NP_4304A"/>
    <dbReference type="EnsemblBacteria" id="CAI50243">
        <property type="protein sequence ID" value="CAI50243"/>
        <property type="gene ID" value="NP_4304A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702026"/>
    <dbReference type="KEGG" id="nph:NP_4304A"/>
    <dbReference type="eggNOG" id="arCOG09048">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2565833_0_0_2"/>
    <dbReference type="OrthoDB" id="242711at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="85" mass="8804" checksum="8DBD6072448EFE4F" modified="2017-05-10" version="1">MAEFTFLEVHLRDANLHAGRSDETADQPVGQSAEQLEPDSDGSRLGAVLAALIGVFFLAAVAYAVKKAVGGEGATDDDTAIADDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYG8</accession>
    <name>A0A1U7EYG8_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Succinate--CoA ligase [ADP-forming] subunit alpha</fullName>
            <ecNumber evidence="5">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Succinyl-CoA synthetase subunit alpha</fullName>
            <shortName evidence="5">SCS-alpha</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 10" type="primary">sucD</name>
        <name evidence="10" type="ordered locus">NP_4354A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 8">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and nucleotide specificity is provided by the beta subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>GTP + succinate + CoA = succinyl-CoA + GDP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:22120"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetate + ATP + CoA = acetyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15081"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 8">
            <text>succinate + ATP + CoA = succinyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5 8">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 8">Heterotetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 7">Belongs to the succinate/malate CoA ligase alpha subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323884.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYG8"/>
    <dbReference type="STRING" id="348780.NP_4354A"/>
    <dbReference type="EnsemblBacteria" id="CAI50268">
        <property type="protein sequence ID" value="CAI50268"/>
        <property type="gene ID" value="NP_4354A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703220"/>
    <dbReference type="KEGG" id="nph:NP_4354A"/>
    <dbReference type="eggNOG" id="arCOG01339">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052104_0_0_2"/>
    <dbReference type="OrthoDB" id="55711at2157"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009361">
        <property type="term" value="C:succinate-CoA ligase complex (ADP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043758">
        <property type="term" value="F:acetate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004776">
        <property type="term" value="F:succinate-CoA ligase (GDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.261:FF:000006">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000277">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01988">
        <property type="entry name" value="Succ_CoA_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017440">
        <property type="entry name" value="Cit_synth/succinyl-CoA_lig_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033847">
        <property type="entry name" value="Citrt_syn/SCS-alpha_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005810">
        <property type="entry name" value="CoA_lig_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004230">
        <property type="entry name" value="PRK05678.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01019">
        <property type="entry name" value="sucCoAalpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11117:SF2">
        <property type="entry name" value="SUCCINATE--COA LIGASE [ADP_GDP-FORMING] SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11117">
        <property type="entry name" value="SUCCINYL-COA LIGASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02629">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001553">
        <property type="entry name" value="SucCS_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01798">
        <property type="entry name" value="SCOASYNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01216">
        <property type="entry name" value="SUCCINYL_COA_LIG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00399">
        <property type="entry name" value="SUCCINYL_COA_LIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 8" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="CoA-binding" evidence="9">
        <location>
            <begin position="4"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="5 6">
        <location>
            <position position="247"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="17"/>
            <end position="20"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="96"/>
            <end position="98"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit beta</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001553-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI50268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="289" mass="29739" checksum="D33B562755D8746E" modified="2017-05-10" version="1">MSILVDNDTRVVVQGITGGEGKFHTGQMLEYGTNVVAGAVPGRGGQEVEGVPVYDTVHKAAREEDANAAVVFVPPAFAADALFEALDSPVDLVVAITEGIPQQDMAKVYRKLRETDTNLIGPNCPGLITPGEAKLGILPGNIFAEGNVGLVSRSGTLTYQVVDSLTQRGIGQTTAVGIGGDPIIGTDFVDALEMFENDPDTEAVVMCGEIGGEDEEEAAEFIGANMDTPVAGFIAGRTAPPGKRMGHAGAIVSGSGTGTAESKINALNNAGVPVGDTPEEVADNIESFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYM2</accession>
    <name>A0A1U7EYM2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF1405 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4530A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323971.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYM2"/>
    <dbReference type="STRING" id="348780.NP_4530A"/>
    <dbReference type="EnsemblBacteria" id="CAI50356">
        <property type="protein sequence ID" value="CAI50356"/>
        <property type="gene ID" value="NP_4530A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702089"/>
    <dbReference type="KEGG" id="nph:NP_4530A"/>
    <dbReference type="eggNOG" id="arCOG02940">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1122605_0_0_2"/>
    <dbReference type="OrthoDB" id="160626at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009845">
        <property type="entry name" value="DUF1405"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40042:SF1">
        <property type="entry name" value="DUF1405 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40042">
        <property type="entry name" value="HYPOTHETICAL MEMBRANE SPANNING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07187">
        <property type="entry name" value="DUF1405"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="232" mass="25843" checksum="CA5592ED3513CEC7" modified="2017-05-10" version="1">MNLRRYLESDVPRDDLPWYLAPLPAALENLALNLAWLVVAVNLAGTAFGFYYYSPQLAQTPVEMWVFVPDSPMATLFMALALASWKLGRPQQWLIALAFFGNIILGGWTVWVHLAFWEQFSYLHPAMRQFLIWSHAAMVAQAFLLHRIGDFSPWAVGAATLWYGVDTTVDYFIPVRGDLHHTFLPADREAAMFLGADALGVAAAGAVLLFVVSVYLALTTHVATLRQRSTAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYP5</accession>
    <name>A0A1U7EYP5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Bifunctional short chain isoprenyl diphosphate synthase</fullName>
            <ecNumber evidence="4">2.5.1.10</ecNumber>
            <ecNumber evidence="4">2.5.1.29</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">idsA1</name>
        <name evidence="4" type="ordered locus">NP_4556A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.10" evidence="4"/>
    <dbReference type="EC" id="2.5.1.29" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323984.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYP5"/>
    <dbReference type="STRING" id="348780.NP_4556A"/>
    <dbReference type="EnsemblBacteria" id="CAI50369">
        <property type="protein sequence ID" value="CAI50369"/>
        <property type="gene ID" value="NP_4556A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702860"/>
    <dbReference type="KEGG" id="nph:NP_4556A"/>
    <dbReference type="eggNOG" id="arCOG01726">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014015_2_1_2"/>
    <dbReference type="OrthoDB" id="26738at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004337">
        <property type="term" value="F:(2E,6E)-farnesyl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004311">
        <property type="term" value="F:geranylgeranyl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001:SF44">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="353" mass="39008" checksum="EB0E88FB21996540" modified="2017-05-10" version="1">MQRVLESWRPAVDETIESVLPRTVDDAYLTSFFGEATYAYDPSALQHALSDPVWELLDRGGKRWRAVLFLLLVEALDDDPDAYLEYAAIPEILHNGTIIVDDVEDGATHRRGDPALHHTHGTDIALNAGNAMYFLPLKIITHNPADLDADTRLAAYEMLMYELNRTHLGQGMDIHWHNQQEIDITEDQYLEMCACKTGCLARIVARLAAIVTNNEAAESDLAAYAEEMAIAFQIADDVLDVEYALEEGGDFGKGVGNDIREGKKTLLTIHAARNASPEAAARLESILWAAENTDEEVAEAIEILESTGSVEYARETAEELSTSAKAHLDDLGLQPDPEAKLRGFADFVVERDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYQ8</accession>
    <name>A0A1U7EYQ8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Probable oxidoreductase (Aldo-keto reductase family protein)</fullName>
            <ecNumber evidence="6">1.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">NP_4562A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYQ8"/>
    <dbReference type="STRING" id="348780.NP_4562A"/>
    <dbReference type="EnsemblBacteria" id="CAI50372">
        <property type="protein sequence ID" value="CAI50372"/>
        <property type="gene ID" value="NP_4562A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_4562A"/>
    <dbReference type="eggNOG" id="arCOG01619">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_0_1_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000002">
        <property type="entry name" value="2,5-diketo-D-gluconic acid reductase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827">
        <property type="entry name" value="2,5-DIKETO-D-GLUCONIC ACID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827:SF3">
        <property type="entry name" value="NADP-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="5">
        <location>
            <begin position="15"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI50372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="273" mass="30368" checksum="AF55F1B04F7233E7" modified="2017-05-10" version="1">MTDAQPPVHDGMPALGLGTWQNEDPDQCAESVRTALEMGYRHIDTAQIYGNEEAVGRGIAAADVDREDIFLATKVWIDSLEYDDVLETTAESLDRLGTDYLDLLYVHWPARTYDPEETLPALEELREDGVIERIGVSNFEPEHIETAQEVLDAPLFANQVEIHPLLQQQRLREFCDENGVELVAYSPLARGDVFGNEVLSDIADDHGVSEAQVSLAWLRQHGLTAIPKATGEGHIADNLASLELSLSESELSRIDDIDDEQRFVDPDFGPDAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYR0</accession>
    <name>A0A1U7EYR0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tritrans,polycis-undecaprenyl-diphosphate synthase (geranylgeranyl-diphosphate specific)</fullName>
            <ecNumber evidence="2">2.5.1.89</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Undecaprenyl diphosphate synthase</fullName>
            <shortName evidence="2">UDS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Undecaprenyl pyrophosphate synthase</fullName>
            <shortName evidence="2">UPP synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">uppS1</name>
        <name evidence="2" type="synonym">uppS</name>
        <name evidence="4" type="ordered locus">NP_4550A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the sequential condensation of isopentenyl diphosphate (IPP) with geranylgeranyl diphosphate (GGPP) to yield (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30E,34E,38E)-undecaprenyl diphosphate (tritrans,heptacis-UPP). It is probably the precursor of glycosyl carrier lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>geranylgeranyl diphosphate + 7 isopentenyl diphosphate = tri-trans,hepta-cis-undecaprenyl diphosphate + 7 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:27622"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57533"/>
            <dbReference type="ChEBI" id="CHEBI:60388"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="2.5.1.89"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.89" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323981.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYR0"/>
    <dbReference type="STRING" id="348780.NP_4550A"/>
    <dbReference type="EnsemblBacteria" id="CAI50366">
        <property type="protein sequence ID" value="CAI50366"/>
        <property type="gene ID" value="NP_4550A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703446"/>
    <dbReference type="KEGG" id="nph:NP_4550A"/>
    <dbReference type="eggNOG" id="arCOG01532">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038505_0_6_2"/>
    <dbReference type="OrthoDB" id="8293at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045547">
        <property type="term" value="F:ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016094">
        <property type="term" value="P:polyprenol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00475">
        <property type="entry name" value="Cis_IPPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="entry name" value="Decaprenyl diphosphate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01139">
        <property type="entry name" value="ISPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001441">
        <property type="entry name" value="UPP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018520">
        <property type="entry name" value="UPP_synth-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00055">
        <property type="entry name" value="uppS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291:SF43">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX SUBUNIT DHDDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01255">
        <property type="entry name" value="Prenyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="Undecaprenyl diphosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01066">
        <property type="entry name" value="UPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2 4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="285"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="32"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="33"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="77"/>
            <end position="79"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="208"/>
            <end position="210"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="306" mass="34204" checksum="95A851256DD7B24D" modified="2017-05-10" version="1">MAPGAGYLSRLYERLLEREVSAAPSHVAVIQDGNRRYAETQGEAATAGHRAGAETTERVLEWCADLGVEELTLYAFSTENFERPPDEREALFDLIAEKLREFADHEEVHDREVRIRAIGQTHRLPDRVREAAAYAERRTEGYDALRLNIALAYGGRAELLDVARDIAAEAAAGDLDPEAVTVDEIESRLTAGPTQAVDLIIRTGGDERTSNFLPWHANGNEAAVYFCTPYWPEFSRIDFLRAIRTYEAREASWERTRAKRAAALVRAFGDRELAEARRVVGQFRGSVPEGELDEVEDPSDRDPSAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYR3</accession>
    <name>A0A1U7EYR3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Ferredoxin (2Fe-2S)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ferA2</name>
        <name evidence="11" type="ordered locus">NP_4568A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 2Fe2S plant-type ferredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323990.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYR3"/>
    <dbReference type="STRING" id="348780.NP_4568A"/>
    <dbReference type="EnsemblBacteria" id="CAI50375">
        <property type="protein sequence ID" value="CAI50375"/>
        <property type="gene ID" value="NP_4568A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702702"/>
    <dbReference type="KEGG" id="nph:NP_4568A"/>
    <dbReference type="eggNOG" id="arCOG02842">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082632_7_3_2"/>
    <dbReference type="OrthoDB" id="212183at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43112">
        <property type="entry name" value="FERREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43112:SF3">
        <property type="entry name" value="FERREDOXIN-2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="10">
        <location>
            <begin position="4"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="86"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI50375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="112" mass="12045" checksum="6E8BDF5F9F44CA20" modified="2017-05-10" version="1">MTDYTVEFVGTGETITVSDKDTILSRCIEEGIAQEFSCRVGMCLACSAEIIEGEVTQPAARGLTDAEREDYALTCMARPQSDLKLDRGKYPPSIEDEVDVDADGTDAAAADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYX1</accession>
    <name>A0A1U7EYX1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">DedA family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">NP_4714A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324062.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYX1"/>
    <dbReference type="STRING" id="348780.NP_4714A"/>
    <dbReference type="EnsemblBacteria" id="CAI50448">
        <property type="protein sequence ID" value="CAI50448"/>
        <property type="gene ID" value="NP_4714A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702154"/>
    <dbReference type="KEGG" id="nph:NP_4714A"/>
    <dbReference type="eggNOG" id="arCOG03117">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1551866_0_0_2"/>
    <dbReference type="OrthoDB" id="204088at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051311">
        <property type="entry name" value="DedA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="VTT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42709">
        <property type="entry name" value="ALKALINE PHOSPHATASE LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42709:SF6">
        <property type="entry name" value="UNDECAPRENYL PHOSPHATE TRANSPORTER A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="VTT_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="VTT" evidence="7">
        <location>
            <begin position="43"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI50448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="168" mass="18453" checksum="D71C13A673DAF1F6" modified="2017-05-10" version="1">MLETLADVILSVLRYVGLPALFVFFIMKGAFIAKPIPTTVILPGYVLVVSADRIETATIILVSTVGFVAGQLLVYYLARREGLSSMQSSSRIHIPSERIDQAERWFKRYGGLGVFVTNFVPYLRGLIFIPAGVTRYPVLPLAFFAFASTLIYHTVIVAIAVGAVRAIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ15</accession>
    <name>A0A1U7EZ15_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cro/C1 family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4796A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324101.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ15"/>
    <dbReference type="STRING" id="348780.NP_4796A"/>
    <dbReference type="EnsemblBacteria" id="CAI50489">
        <property type="protein sequence ID" value="CAI50489"/>
        <property type="gene ID" value="NP_4796A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702179"/>
    <dbReference type="KEGG" id="nph:NP_4796A"/>
    <dbReference type="eggNOG" id="arCOG04060">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077869_0_0_2"/>
    <dbReference type="OrthoDB" id="371772at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017271">
        <property type="entry name" value="Tscrpt_reg_HTH_MJ1545_prd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037724">
        <property type="entry name" value="TF_HTH_MJ1545_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="26"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="234" mass="25616" checksum="04FC7EC7D7645978" modified="2017-05-10" version="1">MREPRAELAERIAGEVTLSDDPGATLRKWREEFDVAQTTLADELDVSASVVSDYESGRRGNPGIQIVQRVVEGLLTVDERRGGDRVRQHARVLSAGFDSDVVYDLREYPATVPIPRFHEAIGAEPVTEGQAETVAGHTVINSVEAIKRLSSEEFYRLYGQSTNRALVFTDVTRGESPLVALRVVTPTPSAVILHGLDADDLWEHAPALARADGVSLATTTMPLEALLEELRSFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ19</accession>
    <name>A0A1U7EZ19_NATPD</name>
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        <submittedName>
            <fullName evidence="2">Cupin 2 barrel domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4800A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324103.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ19"/>
    <dbReference type="STRING" id="348780.NP_4800A"/>
    <dbReference type="EnsemblBacteria" id="CAI50491">
        <property type="protein sequence ID" value="CAI50491"/>
        <property type="gene ID" value="NP_4800A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702180"/>
    <dbReference type="KEGG" id="nph:NP_4800A"/>
    <dbReference type="eggNOG" id="arCOG04684">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2243939_0_0_2"/>
    <dbReference type="OrthoDB" id="211197at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin type-2" evidence="1">
        <location>
            <begin position="31"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="104" mass="11140" checksum="3CAD5F1DE76922AD" modified="2017-05-10" version="1">MTLNELPELSPADGEVETAELVVEDDVLVKLFALGPGGAFDPHVHDDCENVFHLLDGEVIVTQDDTEEHIEAPAVVHNPRGIEHGARNDGDEPALLTASLCPLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ22</accession>
    <name>A0A1U7EZ22_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Trk-type transport system (Probable substrate potassium)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">trkH1</name>
        <name evidence="13" type="synonym">tp29u2a</name>
        <name evidence="13" type="ordered locus">NP_4904A</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TrkH potassium transport family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324155.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ22"/>
    <dbReference type="STRING" id="348780.NP_4904A"/>
    <dbReference type="EnsemblBacteria" id="CAI50543">
        <property type="protein sequence ID" value="CAI50543"/>
        <property type="gene ID" value="NP_4904A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703305"/>
    <dbReference type="KEGG" id="nph:NP_4904A"/>
    <dbReference type="eggNOG" id="arCOG04145">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030708_0_2_2"/>
    <dbReference type="OrthoDB" id="111943at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015379">
        <property type="term" value="F:potassium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003445">
        <property type="entry name" value="Cat_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004772">
        <property type="entry name" value="TrkH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32024">
        <property type="entry name" value="TRK SYSTEM POTASSIUM UPTAKE PROTEIN TRKG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32024:SF2">
        <property type="entry name" value="TRK SYSTEM POTASSIUM UPTAKE PROTEIN TRKG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02386">
        <property type="entry name" value="TrkH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006247">
        <property type="entry name" value="TrkH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="43"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="72"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="137"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="246"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="282"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="344"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="406"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="475"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAI50543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="500" mass="53872" checksum="89CD316BDDE70FB4" modified="2017-05-10" version="1">MVRIRVDWRASLDLTGRVLLYLAAPLCFPLVLAVWYGEALVPFLAAIAVTLALGASLRTLPGDPGNLGPREAFLAVALIWLLVAAVGAIPFIVAGVGTIAHPVNAMFESMSGLTTTGATVLQEFDHHSRSVLMWRQLIQWLGGLGILVLATAILSEIGVGGAQLMERESQTKNVSKLTPRISDTAKLIWGLYVGITLLAVGVYYGLHLAGVAPEMDLYNAIAHAFTSVATAGFSPEPLSIGAFEPIVQWAIIPFMIAGSTSFVLMYIAINGEPMRLLRNDEFHFYLGTLGLFSAIVVAVLVFDGTIDFSSEETIRHALFNVTSIVTTTGYASTDFELWSAAAKYVLFLSMFTGGMVGSTTCSVKSLRWLVALKSFRRNLFTSVHPEAVRPVRLSGSPVDEETIRDIYAYLLLVVVIFAVFAVFVAVDADRAGIGLSAFESMGAAASTFLNIGPAFGEAGPYGTYDIFPMSTKVAMILLMWIGRIEIIPVLVLLTAAFWRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ82</accession>
    <name>A0A1U7EZ82_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Oligoendopeptidase PepF</fullName>
            <ecNumber evidence="9">3.4.24.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pepF</name>
        <name evidence="9" type="ordered locus">NP_5040A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="9"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324221.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ82"/>
    <dbReference type="STRING" id="348780.NP_5040A"/>
    <dbReference type="EnsemblBacteria" id="CAI50611">
        <property type="protein sequence ID" value="CAI50611"/>
        <property type="gene ID" value="NP_5040A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703014"/>
    <dbReference type="KEGG" id="nph:NP_5040A"/>
    <dbReference type="eggNOG" id="arCOG04758">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021290_2_0_2"/>
    <dbReference type="OrthoDB" id="275607at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:metalloendopeptidase activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:peptide metabolic process"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Oligopeptidase f, N-terminal domain"/>
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        <property type="entry name" value="putative peptidase helix hairpin domain like"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Peptidase_M3B"/>
    </dbReference>
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        <property type="entry name" value="pepF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
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    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Oligopeptidase F N-terminal" evidence="8">
        <location>
            <begin position="114"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M3A/M3B catalytic" evidence="7">
        <location>
            <begin position="204"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI50611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="597" mass="68102" checksum="EC698517119646F2" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZA2</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5086A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
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            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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    <dbReference type="EnsemblBacteria" id="CAI50634">
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        <property type="gene ID" value="NP_5086A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702249"/>
    <dbReference type="KEGG" id="nph:NP_5086A"/>
    <dbReference type="eggNOG" id="arCOG07553">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2930294_0_0_2"/>
    <dbReference type="OrthoDB" id="260782at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="entry name" value="DUF7331"/>
    </dbReference>
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        <property type="entry name" value="DUF7331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50634.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="60" mass="6554" checksum="85356DAF28A6208E" modified="2017-05-10" version="1">MSNAPTDTDDGADSRIDPPTTPLDDGQYGHLSLDNGEVVIYDREEPEMWLQSDFAVDLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZC1</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5100A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZC1"/>
    <dbReference type="STRING" id="348780.NP_5100A"/>
    <dbReference type="EnsemblBacteria" id="CAI50641">
        <property type="protein sequence ID" value="CAI50641"/>
        <property type="gene ID" value="NP_5100A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702254"/>
    <dbReference type="KEGG" id="nph:NP_5100A"/>
    <dbReference type="eggNOG" id="arCOG08972">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1222559_0_0_2"/>
    <dbReference type="OrthoDB" id="241839at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057178">
        <property type="entry name" value="DUF7856"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25254">
        <property type="entry name" value="DUF7856"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="32"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="226" mass="24289" checksum="DC260B9FF1BC366D" modified="2017-05-10" version="1">MSLYDHVAVIAPGVTLDRRAALAAAARSNGHKASVADDIERAREQLQSLSASVPTRAAARRRVAETADRLEAERERVATLRGRLEAGDDVADTYRQAIADLSEAETDHAAAKERLDAARERAREARDVRQRRLRLEDELGNLERAARAELAEAVRPAADDAVAALPGCGATTFDGAGPVPAALALARVGSLERPLTLACRHFATSGDAEAWLGVPVVSLRPMVYRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZC4</accession>
    <name>A0A1U7EZC4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Archaea-specific helicase AshA</fullName>
            <ecNumber evidence="7">3.6.4.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ashA</name>
        <name evidence="7" type="ordered locus">NP_5144A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.4.-" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324273.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZC4"/>
    <dbReference type="STRING" id="348780.NP_5144A"/>
    <dbReference type="EnsemblBacteria" id="CAI50663">
        <property type="protein sequence ID" value="CAI50663"/>
        <property type="gene ID" value="NP_5144A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702271"/>
    <dbReference type="KEGG" id="nph:NP_5144A"/>
    <dbReference type="eggNOG" id="arCOG00554">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010611_1_0_2"/>
    <dbReference type="OrthoDB" id="39583at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF1">
        <property type="entry name" value="ATP-DEPENDENT HELICASE MJ1401-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="228"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="421"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI50663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="674" mass="74827" checksum="F74CDF7E0EE2769A" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZF1</accession>
    <name>A0A1U7EZF1_NATPD</name>
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        <submittedName>
            <fullName evidence="3">CopG domain protein</fullName>
        </submittedName>
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        <name evidence="3" type="ordered locus">NP_5152A</name>
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    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <lineage>
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            <taxon>Halobacteriales</taxon>
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            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324277.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZF1"/>
    <dbReference type="STRING" id="348780.NP_5152A"/>
    <dbReference type="EnsemblBacteria" id="CAI50667">
        <property type="protein sequence ID" value="CAI50667"/>
        <property type="gene ID" value="NP_5152A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702274"/>
    <dbReference type="KEGG" id="nph:NP_5152A"/>
    <dbReference type="eggNOG" id="arCOG04800">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121270_0_0_2"/>
    <dbReference type="OrthoDB" id="238179at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002145">
        <property type="entry name" value="CopG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058459">
        <property type="entry name" value="DUF8146"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26471">
        <property type="entry name" value="DUF8146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01402">
        <property type="entry name" value="RHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribbon-helix-helix protein CopG" evidence="2">
        <location>
            <begin position="6"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="86"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="141" mass="16221" checksum="F9118B3D334CA350" modified="2017-05-10" version="1">MGNKNKTISFRVNEEAFETLREIAEERDISLSAVFRDYVDTLVAHDGQVRVVPEHELKSGSASETADAAESFPPKVEVPKSFVREHERLELEAEHLREQLEEYKQYVTKLSQQVEEQEAEDVIQLEDLDGDESEEEPFRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZK8</accession>
    <name>A0A1U7EZK8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_5284A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZK8"/>
    <dbReference type="STRING" id="348780.NP_5284A"/>
    <dbReference type="EnsemblBacteria" id="CAI50733">
        <property type="protein sequence ID" value="CAI50733"/>
        <property type="gene ID" value="NP_5284A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702319"/>
    <dbReference type="KEGG" id="nph:NP_5284A"/>
    <dbReference type="eggNOG" id="arCOG06262">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1222560_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045396">
        <property type="entry name" value="DUF6517"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20127">
        <property type="entry name" value="DUF6517"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="226" mass="24473" checksum="006A8ECD49290EB3" modified="2017-05-10" version="1">MRINVAAFAVLAVVVTAGCAGALLGEDLDFESGETVIEESTAKDAGYELQLSEREEYSQTIDVGDDGNQTNVTVKSHMARYGQVDDDMPAGTGPVQVGTVTIPEATIAGQSVNPLLQMDTYELAARYVGGFDESGFEEHDNYTLQPFDDDSDANVTVFRVEPDEPDAEPTAFIHVLQTQRDDTGDTIVGYVMYPVDEEESETAERLLGALEYTPPEELEDSDAEDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZP9</accession>
    <name>A0A1U7EZP9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3642A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CCI69573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939621.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZP9"/>
    <dbReference type="EnsemblBacteria" id="CCI69573">
        <property type="protein sequence ID" value="CCI69573"/>
        <property type="gene ID" value="NP_3642A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701833"/>
    <dbReference type="KEGG" id="nph:NP_3642A"/>
    <dbReference type="eggNOG" id="arCOG06216">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159080_0_0_2"/>
    <dbReference type="OrthoDB" id="210343at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043828">
        <property type="entry name" value="DUF5805"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19121">
        <property type="entry name" value="DUF5805"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCI69573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="137" mass="15216" checksum="190A01B8DF4C47E7" modified="2017-05-10" version="1">MANGERVPVQTYVTEAQRDIWEREAKQRDMSRAEYVRTMVQAGRRSFELSETGTDTNGDSGNAGDAQVSDATPGVDGLEDRILDVLENEGVADWETLVAGVTDDIESRLDTTLERLQADNRVRYSGRRGGYTVVDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZQ7</accession>
    <name>A0A1U7EZQ7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Small CPxCG-related zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3821C</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CCI69583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZQ7"/>
    <dbReference type="EnsemblBacteria" id="CCI69583">
        <property type="protein sequence ID" value="CCI69583"/>
        <property type="gene ID" value="NP_3821C"/>
    </dbReference>
    <dbReference type="GeneID" id="54763324"/>
    <dbReference type="KEGG" id="nph:NP_3821C"/>
    <dbReference type="eggNOG" id="arCOG06428">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_210071_1_0_2"/>
    <dbReference type="OrthoDB" id="195311at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055985">
        <property type="entry name" value="DUF7563"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24444">
        <property type="entry name" value="DUF7563"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCI69583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="52" mass="5868" checksum="640DD07A192B8FE8" modified="2017-05-10" version="1">MPRCSNCDAFVTDSYVRVFAPQGMSTVRVCPNCEDKLRDGAEVREARSTRQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILS7</accession>
    <name>Q3ILS7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Homolog to HGPV1-ORF14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3330A</name>
        <name evidence="4" type="ordered locus">NP_7054A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4 5">
        <name>PL23</name>
    </geneLocation>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
            <strain evidence="4">Gabara</strain>
            <plasmid evidence="4">PL23</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49756.3"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CR936259">
        <property type="protein sequence ID" value="CAI50943.3"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939594.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILS7"/>
    <dbReference type="STRING" id="348780.NP_3330A"/>
    <dbReference type="EnsemblBacteria" id="CAI49756">
        <property type="protein sequence ID" value="CAI49756"/>
        <property type="gene ID" value="NP_3330A"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI50943">
        <property type="protein sequence ID" value="CAI50943"/>
        <property type="gene ID" value="NP_7054A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703487"/>
    <dbReference type="KEGG" id="nph:NP_3330A"/>
    <dbReference type="KEGG" id="nph:NP_7054A"/>
    <dbReference type="eggNOG" id="arCOG06319">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_504919_0_0_2"/>
    <dbReference type="OrthoDB" id="316855at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057167">
        <property type="entry name" value="DUF7845"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25227">
        <property type="entry name" value="DUF7845"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7845" evidence="2">
        <location>
            <begin position="47"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49756.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50943.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="624" mass="70088" checksum="2BA860A5CCEF0A6C" modified="2012-10-31" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM11</accession>
    <name>Q3IM11_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_6154A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324456.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM11"/>
    <dbReference type="EnsemblBacteria" id="CAI50857">
        <property type="protein sequence ID" value="CAI50857"/>
        <property type="gene ID" value="NP_6154A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694629"/>
    <dbReference type="KEGG" id="nph:NP_6154A"/>
    <dbReference type="HOGENOM" id="CLU_1340763_0_0_2"/>
    <dbReference type="OrthoDB" id="300048at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058700">
        <property type="entry name" value="PIN_com-containing_halobact"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26499">
        <property type="entry name" value="PIN_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="204" mass="23007" checksum="3C60913B4BA4F77A" modified="2005-11-08" version="1">MASEARYPILADTSSLIAVANTTQWERLKDAIHLTTTNVCKHELQNHVNSNKYPPEGSRKHYLKRGGRRVLEHLDEDSSPWSCVTVVPRPHGPDAGEQSLKQELSEHGSSYQIVTILDSAARRSIRRLIDDNGYDIDVVGPPYLLYILLDNDLISKPEFCEATVEMIRTEGWTGYEVVKNAWSSIPVDCSEILDDEYDEALPPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM22</accession>
    <name>Q3IM22_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transducer protein Htr32</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">htr32</name>
        <name evidence="9" type="ordered locus">NP_6128A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9 10">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324445.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM22"/>
    <dbReference type="EnsemblBacteria" id="CAI50844">
        <property type="protein sequence ID" value="CAI50844"/>
        <property type="gene ID" value="NP_6128A"/>
    </dbReference>
    <dbReference type="GeneID" id="28378317"/>
    <dbReference type="KEGG" id="nph:NP_6128A"/>
    <dbReference type="HOGENOM" id="CLU_341856_0_0_2"/>
    <dbReference type="OrthoDB" id="8523at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06225">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11386">
        <property type="entry name" value="MCP_signal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.950">
        <property type="entry name" value="Methyl-accepting chemotaxis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004090">
        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028081">
        <property type="entry name" value="Leu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019968">
        <property type="entry name" value="Urea_ABC_transptr_substrate-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03669">
        <property type="entry name" value="urea_ABC_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47628">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47628:SF1">
        <property type="entry name" value="ALIPHATIC AMIDASE EXPRESSION-REGULATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13458">
        <property type="entry name" value="Peripla_BP_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00260">
        <property type="entry name" value="CHEMTRNSDUCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58104">
        <property type="entry name" value="Methyl-accepting chemotaxis protein (MCP) signaling domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="2 4" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="HAMP" evidence="8">
        <location>
            <begin position="446"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="7">
        <location>
            <begin position="518"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="514"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="810"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="596"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="659"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="514"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI50844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="829" mass="89551" checksum="CE1AFE04071BB906" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM28</accession>
    <name>Q3IM28_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">IS1341-type transposase ISNph13</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">NP_6114A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10 11">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324439.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM28"/>
    <dbReference type="EnsemblBacteria" id="CAI50837">
        <property type="protein sequence ID" value="CAI50837"/>
        <property type="gene ID" value="NP_6114A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694615"/>
    <dbReference type="KEGG" id="nph:NP_6114A"/>
    <dbReference type="HOGENOM" id="CLU_032903_0_1_2"/>
    <dbReference type="OrthoDB" id="33505at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021027">
        <property type="entry name" value="Transposase_put_HTH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12323">
        <property type="entry name" value="HTH_OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0815">Transposition</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Transposase putative helix-turn-helix" evidence="9">
        <location>
            <begin position="6"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="7">
        <location>
            <begin position="189"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="8">
        <location>
            <begin position="307"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI50837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="426" mass="48932" checksum="817BECFC53C58750" modified="2005-11-08" version="1">MDYSPRFRLLPTSRQRELLGWQRDTVRQVYNHALYRLDQLPERADQTVRQRVWSVRDELPDWKTKWEEWSSVYSTVLQAAVERIYHSITGLAQQRDNGHKIGDLNWKKPREFQSFTYRQRGFELDNKSGPEGRGLLTLKKVRGKTLEIPIRIHRDIDPDDIKHVTVKQEATGAWYTSFSIDRDEPEKPDPEDIDPEDTVGLDLGVLNFIHDSDGRSINRLGLSDDRERLEREHRSLSRKEYESNNWEKQRQRVAEVHMDMRNKKRDYKHKLAHAYTTEYDAVFVEDLDVKPMLEDDGNARNKAEVGWRDFIRILKHHGRKHGCHVVEVEPAGTTKECASCGVETAKPLWVREHSCPACGFELGRDFNASLNVLSRGLAKLGVGHSEATTPAETATAVDSVSVSASRVVESGSPCLKEPAKQASRQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM38</accession>
    <name>Q3IM38_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PhoU domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">phoU4</name>
        <name evidence="2" type="synonym">abc15r2</name>
        <name evidence="2" type="ordered locus">NP_6094A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324429.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM38"/>
    <dbReference type="EnsemblBacteria" id="CAI50827">
        <property type="protein sequence ID" value="CAI50827"/>
        <property type="gene ID" value="NP_6094A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694673"/>
    <dbReference type="KEGG" id="nph:NP_6094A"/>
    <dbReference type="HOGENOM" id="CLU_069302_0_0_2"/>
    <dbReference type="OrthoDB" id="40991at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:intracellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045936">
        <property type="term" value="P:negative regulation of phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="entry name" value="Phosphate transport system protein phou homolog 2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028366">
        <property type="entry name" value="PhoU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026022">
        <property type="entry name" value="PhoU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930">
        <property type="entry name" value="PHOSPHATE-SPECIFIC TRANSPORT SYSTEM ACCESSORY PROTEIN PHOU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930:SF2">
        <property type="entry name" value="PHOU DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01895">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00966">
        <property type="entry name" value="SpoVT_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109755">
        <property type="entry name" value="PhoU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="1">
        <location>
            <begin position="18"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="336" mass="37163" checksum="12485479DDD2A71A" modified="2005-11-08" version="1">MTDRTLPDGGPIERKVQVTGGSTYTVSIPKFWANDRDIGSGSPVYLYPFDDRLVVARPDADGQAQRTHIDVDTVGDDAIKKQVEAAYAAGVDEIAVTSDTGFSAVQRRTATRAITNLVGMEIASETERELLAKSLLDPAEISLDDTVDQIRQIALSMHENAVSSVLSSETDEEMTAHIISRDDDVDRLFALVSRQFCRILTDIREVETLRAGRRTVFTQFRVARQLERIADHAERIADVGSRLEAPPSNELASAFEAVAADARRVVRTALDGNTGEALLRRDSVDERIDELDRRLYEDPPPEVYLYGRLLESIRRTAGYGGNIAEIITLDRIMGDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM46</accession>
    <name>Q3IM46_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">UPF0061 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">NP_6074A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10 11">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SELO family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324421.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM46"/>
    <dbReference type="EnsemblBacteria" id="CAI50817">
        <property type="protein sequence ID" value="CAI50817"/>
        <property type="gene ID" value="NP_6074A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694702"/>
    <dbReference type="KEGG" id="nph:NP_6074A"/>
    <dbReference type="HOGENOM" id="CLU_010245_4_1_2"/>
    <dbReference type="OrthoDB" id="315286at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070733">
        <property type="term" value="F:AMPylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00692">
        <property type="entry name" value="SelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003846">
        <property type="entry name" value="SelO"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000658">
        <property type="entry name" value="PRK00029.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32057">
        <property type="entry name" value="PROTEIN ADENYLYLTRANSFERASE SELO, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32057:SF14">
        <property type="entry name" value="PROTEIN ADENYLYLTRANSFERASE SELO, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02696">
        <property type="entry name" value="SelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="440"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI50817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="464" mass="52871" checksum="605723CEC4EADCA1" modified="2005-11-08" version="1">MAFSFDTSYKDLDSKLYSGATPKNIDDPEVLVLNADLCDALGLNKEDLDSQLLSGQDTLEAPIAQAYAGHQFGNFTVLGDGRAMVLGEHVHDGSRYDIQLKGSGRTPYSGRGDGNATVSSMLREYIYSYSMQNLGIKTSRSLSVVETEESVQRRRTEPGAVLVRVMNSHIRYGTFQYVAHRAPEELASFTDYVIDRHYPHLDGADKKYVKFFDEVMRSSIDMVVDWMRVGFIHGVMNTDNMSIDGETFDYGPCAFMNYYDEDTVFSSVDKRGRYSFGSQRDILKWNLERFAESLKPLFDESAHGFDELEATLGNFDETFDAKYYSMMRKKLGIKSDGEKPLIDRFVDWLRESGADYTNTFLELENPGTFAAPVYSSTEFNHLQSELAAVGLDKQLMAEANPRYIPRNYLVEEALNEYLEKKDLSKFRRLLDVLENPYESKDVDSQFQQPPPEEFDSKYTTYCNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM51</accession>
    <name>Q3IM51_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sensor box histidine kinase</fullName>
            <ecNumber evidence="8">2.7.13.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">NP_6064A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8 9">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.13.3" evidence="8"/>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324416.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM51"/>
    <dbReference type="EnsemblBacteria" id="CAI50812">
        <property type="protein sequence ID" value="CAI50812"/>
        <property type="gene ID" value="NP_6064A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694697"/>
    <dbReference type="KEGG" id="nph:NP_6064A"/>
    <dbReference type="HOGENOM" id="CLU_000445_114_58_2"/>
    <dbReference type="OrthoDB" id="230688at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004673">
        <property type="term" value="F:protein histidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47429">
        <property type="entry name" value="PROTEIN TWIN LOV 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47429:SF2">
        <property type="entry name" value="PROTEIN TWIN LOV 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0157">Chromophore</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0288">FMN</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="6">
        <location>
            <begin position="128"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="7">
        <location>
            <begin position="204"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="6">
        <location>
            <begin position="253"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="5">
        <location>
            <begin position="382"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI50812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="596" mass="65502" checksum="94318C33F55C87A6" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM65</accession>
    <name>Q3IM65_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">HTH-10 family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_6030A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5 6">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM65"/>
    <dbReference type="EnsemblBacteria" id="CAI50795">
        <property type="protein sequence ID" value="CAI50795"/>
        <property type="gene ID" value="NP_6030A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_6030A"/>
    <dbReference type="HOGENOM" id="CLU_099316_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007050">
        <property type="entry name" value="HTH_bacterioopsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056486">
        <property type="entry name" value="HVO_2525_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236:SF1">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04967">
        <property type="entry name" value="HTH_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24279">
        <property type="entry name" value="HVO_2525_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="Sigma3 and sigma4 domains of RNA polymerase sigma factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HVO-2525 N-terminal" evidence="4">
        <location>
            <begin position="4"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="HTH bat-type" evidence="3">
        <location>
            <begin position="171"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI50795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="250" mass="28569" checksum="57B145AFB0CA41EC" modified="2005-11-08" version="1">MKSLKLDMVQYDCPYIRTSKSHDVAFRTQHWEFNQANRTLETRLLTIGEDSEELHSALEALNSCEMLQDYDLLSRKRNTALLRSRIDETDAMRTIRKNGGYVTGPFVIRNGSEIWNVGFDRGQAAGDALSELDRSNEFSIKSDDSVHIDDFFDILQNVESLGSLLRTLQSLTDRERETLETAVEDGYFATPRGTTLDGIGEEFDISRMGASKNLRRSQRKVLRKVIHVLNDVEAQMELQEEGPETQELDE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IQC4</accession>
    <name>TRPA_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tryptophan synthase alpha chain</fullName>
            <ecNumber evidence="1">4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trpA</name>
        <name type="ordered locus">NP_3162A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text>The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + L-tryptophan + H2O</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TrpA family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323294.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IQC4"/>
    <dbReference type="SMR" id="Q3IQC4"/>
    <dbReference type="STRING" id="348780.NP_3162A"/>
    <dbReference type="EnsemblBacteria" id="CAI49672">
        <property type="protein sequence ID" value="CAI49672"/>
        <property type="gene ID" value="NP_3162A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703407"/>
    <dbReference type="KEGG" id="nph:NP_3162A"/>
    <dbReference type="eggNOG" id="arCOG01086">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016734_0_0_2"/>
    <dbReference type="OrthoDB" id="25658at2157"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00044"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04724">
        <property type="entry name" value="Tryptophan_synthase_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000037">
        <property type="entry name" value="Tryptophan synthase alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00131">
        <property type="entry name" value="Trp_synth_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018204">
        <property type="entry name" value="Trp_synthase_alpha_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002028">
        <property type="entry name" value="Trp_synthase_suA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00262">
        <property type="entry name" value="trpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43406:SF1">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE ALPHA CHAIN, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43406">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE, ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00290">
        <property type="entry name" value="Trp_syntA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00167">
        <property type="entry name" value="TRP_SYNTHASE_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="chain" id="PRO_1000018236" description="Tryptophan synthase alpha chain">
        <location>
            <begin position="1"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="42"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00131"/>
        </source>
    </evidence>
    <sequence length="277" mass="29360" checksum="6A3BD9CAA2E076EE" modified="2005-11-08" version="1">MSLEDAFDEPAFVPYLAAGDPDFESSLAYVEALARGGADVIELGLPFSEPIAEGPTIQQAVVRSLEGGMTPERFFEFVETLDVDVPLVCMTYYNLIYQYGPAGSQPVVPFVERAAEVGLKGFVVPDLPAEEAGPLREACDEYGLDLVFIVAPTTAGDRLERMMEQVSGYVYVQARLGVTGAREDVSDRTAETLARLEAYDVPKAVGFGISSGEQAEAVVAAGADGVIVGSALVDIVAEGHENGDAPDVVADRLESLARELKSGAVAGKQRQPGPERT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-01-10" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IQR5</accession>
    <name>HISX_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Histidinol dehydrogenase</fullName>
            <shortName evidence="1">HDH</shortName>
            <ecNumber evidence="1">1.1.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisD</name>
        <name type="ordered locus">NP_2876A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the sequential NAD-dependent oxidations of L-histidinol to L-histidinaldehyde and then to L-histidine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-histidinol + 2 NAD(+) + H2O = L-histidine + 2 NADH + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:20641"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:57699"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 9/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the histidinol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.23" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323154.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IQR5"/>
    <dbReference type="SMR" id="Q3IQR5"/>
    <dbReference type="STRING" id="348780.NP_2876A"/>
    <dbReference type="EnsemblBacteria" id="CAI49529">
        <property type="protein sequence ID" value="CAI49529"/>
        <property type="gene ID" value="NP_2876A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702817"/>
    <dbReference type="KEGG" id="nph:NP_2876A"/>
    <dbReference type="eggNOG" id="arCOG04352">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006732_3_0_2"/>
    <dbReference type="OrthoDB" id="36308at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00014"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004399">
        <property type="term" value="F:histidinol dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06572">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1980:FF:000001">
        <property type="entry name" value="Histidinol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1980:FF:000026">
        <property type="entry name" value="Histidinol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1980">
        <property type="entry name" value="Nitrogenase molybdenum iron protein domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01024">
        <property type="entry name" value="HisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001692">
        <property type="entry name" value="Histidinol_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022695">
        <property type="entry name" value="Histidinol_DH_monofunct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012131">
        <property type="entry name" value="Hstdl_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00069">
        <property type="entry name" value="hisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21256:SF2">
        <property type="entry name" value="HISTIDINE BIOSYNTHESIS TRIFUNCTIONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21256">
        <property type="entry name" value="HISTIDINOL DEHYDROGENASE HDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00815">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000099">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00083">
        <property type="entry name" value="HOLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00611">
        <property type="entry name" value="HISOL_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000135900" description="Histidinol dehydrogenase">
        <location>
            <begin position="1"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="320"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="321"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="354"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="354"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="408"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="413"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="413"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01024"/>
        </source>
    </evidence>
    <sequence length="422" mass="44465" checksum="EDC37AE445D0D7F5" modified="2005-11-08" version="1">MNVSAVADLSPEARAALFERDAGIEDARESVRDIIGRVREEGDVALRSYAKEFDDVDIGTLAVTDEAERAYDDLDDDLRDAIETAAENIRTFHERQVPDDWREQFDGRELGRRYRPLERVGVYAPGGTAAYPSSVLMGVIPAVVAGVDHVAVATPPAEELPAATLAAAHVAGADAVYQVGGAQAIAALAYGTESVSAVQKVVGPGNRWVTAAKAEVRGDVEIDFLAGPSELLVVCDDTADPELIAADMVAQAEHDPNASVVCVSDDEATAEAVADACERQATARERTEAIESALENDASAVLCARSMSEAVLFAEEYAAEHLSIVAADEEALLDRIDSAGSVFLGGFSPVAVGDYASGTNHVLPTGGLAKVAGGLSVDHFVRSTTVQKLDEDALSSLRETVTTLARAEGLEAHAESVDKRFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IRS9</accession>
    <name>Q3IRS9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Probable archaetidylglycerolphosphate synthase</fullName>
            <ecNumber evidence="4">2.7.8.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">agsA</name>
        <name evidence="4" type="synonym">pgsA2</name>
        <name evidence="4" type="ordered locus">NP_2144A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.-" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322791.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IRS9"/>
    <dbReference type="STRING" id="348780.NP_2144A"/>
    <dbReference type="DNASU" id="3703021"/>
    <dbReference type="EnsemblBacteria" id="CAI49163">
        <property type="protein sequence ID" value="CAI49163"/>
        <property type="gene ID" value="NP_2144A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703021"/>
    <dbReference type="KEGG" id="nph:NP_2144A"/>
    <dbReference type="eggNOG" id="arCOG00670">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080384_1_2_2"/>
    <dbReference type="OrthoDB" id="9904at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016780">
        <property type="term" value="F:phosphotransferase activity, for other substituted phosphate groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2 4" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="33"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="104"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="209" mass="21678" checksum="4852720765477BBF" modified="2005-11-08" version="1">MTLDKLRPVAERVLNPFVAAADRVGLTPDGVSVIAFGFAVAAAAAYTAAGTAVVGVGPDPALYLLGALFVLGNGWMDLLDGALARRQGTDSKAGDLLDHVLDRYADIVVVAGLAAGAESYLLGLLAVTGVLMTSYLGTQAQAVGLDRVYGGLVGRADRLAVIGIVTAIAAGYPEPLWTLSLVEWLLVFLAVVGHLTALQRFYHSMRALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ISN6</accession>
    <name>Q3ISN6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA double-strand break repair protein Mre11</fullName>
            <ecNumber evidence="9">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 12" type="primary">mre11</name>
        <name evidence="12" type="ordered locus">NP_1518A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of the Rad50/Mre11 complex, which is involved in the early steps of DNA double-strand break (DSB) repair. Mre11 binds to DSB ends and has both double-stranded 3'-5' exonuclease activity and single-stranded endonuclease activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="9">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">Nuclease activity is regulated by Rad50.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer. Forms a heterotetramer composed of two Mre11 subunits and two Rad50 subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the MRE11/RAD32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="9"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322484.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ISN6"/>
    <dbReference type="STRING" id="348780.NP_1518A"/>
    <dbReference type="EnsemblBacteria" id="CAI48850">
        <property type="protein sequence ID" value="CAI48850"/>
        <property type="gene ID" value="NP_1518A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701167"/>
    <dbReference type="KEGG" id="nph:NP_1518A"/>
    <dbReference type="eggNOG" id="arCOG00397">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026621_3_0_2"/>
    <dbReference type="OrthoDB" id="11638at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045027">
        <property type="term" value="F:DNA end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000403">
        <property type="term" value="F:Y-form DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00840">
        <property type="entry name" value="MPP_Mre11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02044">
        <property type="entry name" value="Mre11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050535">
        <property type="entry name" value="DNA_Repair-Maintenance_Comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032885">
        <property type="entry name" value="Mre11_archaea-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054879">
        <property type="entry name" value="Mre11_Halo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041796">
        <property type="entry name" value="Mre11_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041030">
        <property type="entry name" value="Mre11_Halo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30337">
        <property type="entry name" value="COMPONENT OF ATP-DEPENDENT DSDNA EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30337:SF0">
        <property type="entry name" value="NUCLEASE SBCCD SUBUNIT D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6 9" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5 9 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 9" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="11">
        <location>
            <begin position="3"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="364"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="371"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="427"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="9">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI48850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="451" mass="49180" checksum="8D40DEC1248CC42E" modified="2005-11-08" version="1">MTRVLHTGDTHIGYRQYHTPERREDFLSAFRQVADDAVEMDVDAVVHAGDLFHDRRPGLVDLLGTVDILETLDSAGIPFLAIVGNHETKRDAQWLDLFETMGLATRLGDEPVPVGETAFYGLDFVPRNQRDDLEYEFATHDCEHAALVSHGLFEPLVPDYGNVEWDIEAVLEASNIDFDAVLLGDEHAATKQEVADTWVTYCGSTERTSASERDDRGYNLVTFDGAVRISRRGIKTRPFVFVDLELGATEGFNYVRDRLLEYDLDDAVVRVTLDGDGEDVSPARIESFATDEGALVARVTDNRTIDVDADAPDIAFADPDKAARERVRELGLSEMGRQLDETVRDGEIADSNLSDEIERRVESALEDGGLEDIAGTAADADTGDNGEAGDSGATTGSESERADGKTGPSDTGDAGETGDARTEAESNESSQTQETDGQPAENGQSSMEEYL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ITB2</accession>
    <name>HIS1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP phosphoribosyltransferase</fullName>
            <shortName evidence="1">ATP-PRT</shortName>
            <shortName evidence="1">ATP-PRTase</shortName>
            <ecNumber evidence="1">2.4.2.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisG</name>
        <name type="ordered locus">NP_1062A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of ATP and 5-phosphoribose 1-diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of HisG enzymatic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + ATP</text>
            <dbReference type="Rhea" id="RHEA:18473"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:73183"/>
            <dbReference type="EC" id="2.4.2.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Feedback inhibited by histidine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP phosphoribosyltransferase family. Long subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.17" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322258.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ITB2"/>
    <dbReference type="SMR" id="Q3ITB2"/>
    <dbReference type="STRING" id="348780.NP_1062A"/>
    <dbReference type="EnsemblBacteria" id="CAI48622">
        <property type="protein sequence ID" value="CAI48622"/>
        <property type="gene ID" value="NP_1062A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702820"/>
    <dbReference type="KEGG" id="nph:NP_1062A"/>
    <dbReference type="eggNOG" id="arCOG02208">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038115_1_1_2"/>
    <dbReference type="OrthoDB" id="33116at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003879">
        <property type="term" value="F:ATP phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.120:FF:000002">
        <property type="entry name" value="ATP phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000008">
        <property type="entry name" value="ATP phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00079">
        <property type="entry name" value="HisG_Long"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020621">
        <property type="entry name" value="ATP-PRT_HisG_long"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013820">
        <property type="entry name" value="ATP_PRibTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018198">
        <property type="entry name" value="ATP_PRibTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001348">
        <property type="entry name" value="ATP_PRibTrfase_HisG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013115">
        <property type="entry name" value="HisG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00070">
        <property type="entry name" value="hisG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03455">
        <property type="entry name" value="HisG_C-term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21403:SF10">
        <property type="entry name" value="ATP PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21403">
        <property type="entry name" value="ATP PHOSPHORIBOSYLTRANSFERASE ATP-PRTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01634">
        <property type="entry name" value="HisG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08029">
        <property type="entry name" value="HisG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="GlnB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01316">
        <property type="entry name" value="ATP_P_PHORIBOSYLTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000004480" description="ATP phosphoribosyltransferase">
        <location>
            <begin position="1"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00079"/>
        </source>
    </evidence>
    <sequence length="281" mass="30175" checksum="B5E7FE702EF48A77" modified="2005-11-08" version="1">MRIAVPNKGRLHEPALELLERAGLHVQDGSDRKLYADTVDPEVSVLFARAADIPEYVADGAAALGITGLDQERESETDLVDLLDLEFGSCRLVLASPEDGGVSAPEELSGGTVATEFPKITERYFEELGVRPDIVEVSGATELTPHVDIADAIVDITSTGTTLRMNRLEVVDEVLESSVRLFADPAVADDPKVEQVTTAFRSVLDADGKRYLMMNVPEDSLDAVEAEIPGMGGPTVMDVAGQDTVAVHVVVDEREVFEVIPKLKAAGATDILVTEIERLVP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-26" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ITC7</accession>
    <name>AATB_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">A-type ATP synthase subunit B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpB</name>
        <name type="ordered locus">NP_1032A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane. The B chain is a regulatory subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Has multiple subunits with at least A(3), B(3), C, D, E, F, H, I and proteolipid K(x).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322242.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ITC7"/>
    <dbReference type="SMR" id="Q3ITC7"/>
    <dbReference type="STRING" id="348780.NP_1032A"/>
    <dbReference type="EnsemblBacteria" id="CAI48607">
        <property type="protein sequence ID" value="CAI48607"/>
        <property type="gene ID" value="NP_1032A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702539"/>
    <dbReference type="KEGG" id="nph:NP_1032A"/>
    <dbReference type="eggNOG" id="arCOG00865">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022916_0_0_2"/>
    <dbReference type="OrthoDB" id="32941at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033178">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, catalytic domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18112">
        <property type="entry name" value="ATP-synt_V_A-type_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18118">
        <property type="entry name" value="ATP-synt_V_A-type_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01135">
        <property type="entry name" value="V_A-ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00310">
        <property type="entry name" value="ATP_synth_B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005724">
        <property type="entry name" value="ATPase_A1-cplx_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022879">
        <property type="entry name" value="V-ATPase_su_B/beta"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01041">
        <property type="entry name" value="ATP_syn_B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003235">
        <property type="entry name" value="PRK04196.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389:SF4">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039114">
        <property type="entry name" value="V-ATPsynth_beta/V-ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47917">
        <property type="entry name" value="C-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000322506" description="A-type ATP synthase subunit B">
        <location>
            <begin position="1"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00310"/>
        </source>
    </evidence>
    <sequence length="471" mass="52364" checksum="25F5ABA4EBCCB0B9" modified="2005-11-08" version="1">MQKEYKTITEISGPLVFAEVDEPVGYDEMVEIETPSGETRRGQVLESTSEFVAIQVFEGTSGIDRNSSVRFLGETLQMPLTEELLGRVLSGSGEPIDGGPEIEPDEEREIVGAAINPTAREYPEEFIQTGVSAIDGMNTLIRGQKLPIFSGSGLPHNELALQIARQASVPEEESGDDEAGSEFAVIFGAMGITQEEANEFMDDFERTGALERSVVFMNLADDPAVERTVTPRMALTTAEYLAFEEGYHVLVILTDMTNYCEALREIGAAREEVPGRRGYPGYMYTDLAQLYERAGRIEGREGSVTQIPILTMPGDDDTHPIPDLTGYITEGQIYIDRNLNSQGIEPPINVLPSLSRLMDDGIGEGFTREDHPDVSDQAYAAYAEGEDLRDLVNIVGREALSERDNKYLDFADRFEEEFVQQGYDTNRDVEETLDLAWELLSDLPKEELNRIDEEAIEEYYVEDEQAAQTAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ITM3</accession>
    <name>Q3ITM3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Mrp-type sodium/proton antiporter system subunit G</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">mrpG</name>
        <name evidence="2" type="synonym">tp49c</name>
        <name evidence="2" type="ordered locus">NP_0838A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322146.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ITM3"/>
    <dbReference type="STRING" id="348780.NP_0838A"/>
    <dbReference type="EnsemblBacteria" id="CAI48510">
        <property type="protein sequence ID" value="CAI48510"/>
        <property type="gene ID" value="NP_0838A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703328"/>
    <dbReference type="KEGG" id="nph:NP_0838A"/>
    <dbReference type="eggNOG" id="arCOG03082">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121334_0_6_2"/>
    <dbReference type="OrthoDB" id="19138at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005133">
        <property type="entry name" value="PhaG_MnhG_YufB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01300">
        <property type="entry name" value="CPA3_mnhG_phaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009314">
        <property type="entry name" value="PRK12674.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34703">
        <property type="entry name" value="ANTIPORTER SUBUNIT MNHG2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34703:SF1">
        <property type="entry name" value="ANTIPORTER SUBUNIT MNHG2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03334">
        <property type="entry name" value="PhaG_MnhG_YufB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="118" mass="12146" checksum="F8C36BFC3C74149F" modified="2005-11-08" version="1">MTLVTALIVLLVVLGVFFTLVAAVGVLRLPDVYTRSHAATKADTLGAGFAIAAVGLYFGTAGELVRAGLLLVFIYVTNPTAAHAITRAAYSQDVDVWTVDDNDTGTDTASAADGGDSQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-07-10" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IU54</accession>
    <name>DP2L_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA polymerase II large subunit</fullName>
            <shortName evidence="2">Pol II</shortName>
            <ecNumber evidence="2">2.7.7.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Exodeoxyribonuclease large subunit</fullName>
            <ecNumber evidence="2">3.1.11.1</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">polC</name>
        <name type="synonym">polA2</name>
        <name type="ordered locus">NP_0476A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer of a large subunit and a small subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal DNA polymerase II family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="2"/>
    <dbReference type="EC" id="3.1.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321965.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IU54"/>
    <dbReference type="SMR" id="Q3IU54"/>
    <dbReference type="STRING" id="348780.NP_0476A"/>
    <dbReference type="EnsemblBacteria" id="CAI48329">
        <property type="protein sequence ID" value="CAI48329"/>
        <property type="gene ID" value="NP_0476A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703041"/>
    <dbReference type="KEGG" id="nph:NP_0476A"/>
    <dbReference type="eggNOG" id="arCOG04447">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001154_0_0_2"/>
    <dbReference type="OrthoDB" id="7529at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008310">
        <property type="term" value="F:single-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00324">
        <property type="entry name" value="DNApol_II_L_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004475">
        <property type="entry name" value="PolC_DP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056172">
        <property type="entry name" value="PolC_DP2_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056171">
        <property type="entry name" value="PolC_DP2_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016033">
        <property type="entry name" value="PolC_DP2_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00354">
        <property type="entry name" value="polC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003103">
        <property type="entry name" value="PRK04023.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42210">
        <property type="entry name" value="DNA POLYMERASE II LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42210:SF1">
        <property type="entry name" value="DNA POLYMERASE II LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24846">
        <property type="entry name" value="PolC_DP2_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24844">
        <property type="entry name" value="PolC_DP2_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03833">
        <property type="entry name" value="PolC_DP2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016275">
        <property type="entry name" value="PolC_DP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword id="KW-0269">Exonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000294698" description="DNA polymerase II large subunit">
        <location>
            <begin position="1"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="281"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="534"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="286"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="1198" mass="132156" checksum="0F886B4A2B484FFA" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IUE8</accession>
    <name>METXA_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Homoserine O-acetyltransferase</fullName>
            <shortName evidence="1 3">HAT</shortName>
            <ecNumber evidence="1 2">2.3.1.31</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Homoserine transacetylase</fullName>
            <shortName evidence="1">HTA</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1 3" type="primary">metXA</name>
        <name type="ordered locus">NP_0282A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2017" name="Nat. Chem. Biol." volume="13" first="858" last="866">
            <title>Parallel evolution of non-homologous isofunctional enzymes in methionine biosynthesis.</title>
            <authorList>
                <person name="Bastard K."/>
                <person name="Perret A."/>
                <person name="Mariage A."/>
                <person name="Bessonnet T."/>
                <person name="Pinet-Turpault A."/>
                <person name="Petit J.L."/>
                <person name="Darii E."/>
                <person name="Bazire P."/>
                <person name="Vergne-Vaxelaire C."/>
                <person name="Brewee C."/>
                <person name="Debard A."/>
                <person name="Pellouin V."/>
                <person name="Besnard-Gonnet M."/>
                <person name="Artiguenave F."/>
                <person name="Medigue C."/>
                <person name="Vallenet D."/>
                <person name="Danchin A."/>
                <person name="Zaparucha A."/>
                <person name="Weissenbach J."/>
                <person name="Salanoubat M."/>
                <person name="de Berardinis V."/>
            </authorList>
            <dbReference type="PubMed" id="28581482"/>
            <dbReference type="DOI" id="10.1038/nchembio.2397"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2">Transfers an acetyl group from acetyl-CoA to L-homoserine, forming acetyl-L-homoserine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>L-homoserine + acetyl-CoA = O-acetyl-L-homoserine + CoA</text>
            <dbReference type="Rhea" id="RHEA:13701"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57716"/>
            <dbReference type="EC" id="2.3.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; O-acetyl-L-homoserine from L-homoserine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. MetX family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.31" evidence="1 2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321871.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IUE8"/>
    <dbReference type="SMR" id="Q3IUE8"/>
    <dbReference type="STRING" id="348780.NP_0282A"/>
    <dbReference type="ESTHER" id="natpd-metx">
        <property type="family name" value="Homoserine_transacetylase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI48232">
        <property type="protein sequence ID" value="CAI48232"/>
        <property type="gene ID" value="NP_0282A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702915"/>
    <dbReference type="KEGG" id="nph:NP_0282A"/>
    <dbReference type="eggNOG" id="arCOG00627">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028760_1_2_2"/>
    <dbReference type="OrthoDB" id="295172at2157"/>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00074"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004414">
        <property type="term" value="F:homoserine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009092">
        <property type="term" value="P:homoserine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00296">
        <property type="entry name" value="MetX_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008220">
        <property type="entry name" value="HAT_MetX-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01392">
        <property type="entry name" value="homoserO_Ac_trn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001209">
        <property type="entry name" value="PRK00175.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32268">
        <property type="entry name" value="HOMOSERINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32268:SF11">
        <property type="entry name" value="HOMOSERINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000443">
        <property type="entry name" value="Homoser_Ac_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0486">Methionine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000231893" description="Homoserine O-acetyltransferase">
        <location>
            <begin position="1"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="37"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="318"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="351"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="352"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="28581482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="28581482"/>
        </source>
    </evidence>
    <sequence length="401" mass="43429" checksum="64B15DBFED425613" modified="2005-11-08" version="1">MSEQQTASLGEFTFECGESIPDLELAYETYGEFTGDNAVLVCHALTGSAHVAGGRRRSDTSGQAHAWWDDIVGPGKAVDTTDYYVICANIPGSCYGSSGPPSTNPETGEPWGTTFPAVTVGDWTRAQRRLLDHLGVPHLHAVVGGSVGGMNVLDWAKRHPDHVNRVAVVAAAARLDPQCLALDAIARRAITTDPNWNGGDYYGEDPPVEGLALARQIGHVMYLSKSSMQDKFGRRSSGIDAGRDSFEMDPAAGFFPYREVESYLDYQGEKFARRFDANSYLYLTRAMDNYDLASGYESDRDALAAFDGEALLISFTGDWHFTVEQSEAVADAFRESGTDTAHHVVDSDHGHDAFLVEPDRVGPPLDDFLVDGVAGKAVSDTTPDDDDGDEFAPVHTSLFSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IUF3</accession>
    <name>Q3IUF3_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">D-3-phosphoglycerate dehydrogenase</fullName>
            <ecNumber evidence="3 10">1.1.1.95</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">serA1</name>
        <name evidence="14" type="ordered locus">NP_0272A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>(2R)-3-phosphoglycerate + NAD(+) = 3-phosphooxypyruvate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12641"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18110"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="EC" id="1.1.1.95"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.95" evidence="3 10"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321866.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IUF3"/>
    <dbReference type="STRING" id="348780.NP_0272A"/>
    <dbReference type="EnsemblBacteria" id="CAI48227">
        <property type="protein sequence ID" value="CAI48227"/>
        <property type="gene ID" value="NP_0272A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703205"/>
    <dbReference type="KEGG" id="nph:NP_0272A"/>
    <dbReference type="eggNOG" id="arCOG01754">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019796_8_1_2"/>
    <dbReference type="OrthoDB" id="7437at2157"/>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00196"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004617">
        <property type="term" value="F:phosphoglycerate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04902">
        <property type="entry name" value="ACT_3PGDH-xct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12173">
        <property type="entry name" value="PGDH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.90:FF:000003">
        <property type="entry name" value="D-3-phosphoglycerate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000021">
        <property type="entry name" value="D-3-phosphoglycerate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.260:FF:000008">
        <property type="entry name" value="D-3-phosphoglycerate dehydrogenase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.90">
        <property type="entry name" value="D-3-phosphoglycerate dehydrogenase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029009">
        <property type="entry name" value="ASB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050857">
        <property type="entry name" value="D-2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006236">
        <property type="entry name" value="PGDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045626">
        <property type="entry name" value="PGDH_ASB_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01327">
        <property type="entry name" value="PGDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42789">
        <property type="entry name" value="D-ISOMER SPECIFIC 2-HYDROXYACID DEHYDROGENASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G10090)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42789:SF1">
        <property type="entry name" value="D-ISOMER SPECIFIC 2-HYDROXYACID DEHYDROGENASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G10090)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19304">
        <property type="entry name" value="PGDH_inter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143548">
        <property type="entry name" value="Serine metabolism enzymes domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00670">
        <property type="entry name" value="D_2_HYDROXYACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0520">NAD</keyword>
    <keyword evidence="6 10 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0718">Serine biosynthesis</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="11">
        <location>
            <begin position="3"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="12">
        <location>
            <begin position="105"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="D-3-phosphoglycerate dehydrogenase ASB" evidence="13">
        <location>
            <begin position="323"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF19304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAI48227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="526" mass="55551" checksum="4FDABC8F443A01A5" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IUK9</accession>
    <name>Q3IUK9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Type IV pilus biogenesis complex ATPase subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">pilB2</name>
        <name evidence="3" type="ordered locus">NP_0160A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GSP E family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321810.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IUK9"/>
    <dbReference type="STRING" id="348780.NP_0160A"/>
    <dbReference type="EnsemblBacteria" id="CAI48171">
        <property type="protein sequence ID" value="CAI48171"/>
        <property type="gene ID" value="NP_0160A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700778"/>
    <dbReference type="KEGG" id="nph:NP_0160A"/>
    <dbReference type="eggNOG" id="arCOG01818">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023388_0_0_2"/>
    <dbReference type="OrthoDB" id="262046at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050921">
        <property type="entry name" value="T4SS_GSP_E_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30486:SF14">
        <property type="entry name" value="FLAGELLA ACCESSORY PROTEIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30486">
        <property type="entry name" value="TWITCHING MOTILITY PROTEIN PILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bacterial type II secretion system protein E" evidence="2">
        <location>
            <begin position="379"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="720" mass="80316" checksum="4A6B0177D74E9806" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-04-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1Q2UA73</accession>
    <name>A0A1Q2UA73_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2344A</name>
        <name evidence="2" type="ordered locus">NP_5346A</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
            <strain evidence="1">Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49263.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50764.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1Q2UA73"/>
    <dbReference type="EnsemblBacteria" id="CAI49263">
        <property type="protein sequence ID" value="CAI49263"/>
        <property type="gene ID" value="NP_2344A"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI50764">
        <property type="protein sequence ID" value="CAI50764"/>
        <property type="gene ID" value="NP_5346A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_2344A"/>
    <dbReference type="KEGG" id="nph:NP_5346A"/>
    <dbReference type="HOGENOM" id="CLU_3408365_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49263.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50764.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="29" mass="3001" checksum="B8A0DA0B49A6E14E" modified="2017-04-12" version="1">MLRLLGTVAAVGLSAWALETAGYIDVLPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETD9</accession>
    <name>A0A1U7ETD9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Receiver box response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_0140A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48161.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321800.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETD9"/>
    <dbReference type="STRING" id="348780.NP_0140A"/>
    <dbReference type="EnsemblBacteria" id="CAI48161">
        <property type="protein sequence ID" value="CAI48161"/>
        <property type="gene ID" value="NP_0140A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700768"/>
    <dbReference type="KEGG" id="nph:NP_0140A"/>
    <dbReference type="eggNOG" id="arCOG02589">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_17_2"/>
    <dbReference type="OrthoDB" id="9652at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17557">
        <property type="entry name" value="REC_Rcp-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052893">
        <property type="entry name" value="TCS_response_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44520:SF2">
        <property type="entry name" value="RESPONSE REGULATOR RCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44520">
        <property type="entry name" value="RESPONSE REGULATOR RCP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="26"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48161.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="159" mass="17445" checksum="C2447523E0130816" modified="2017-05-10" version="1">MPEPADVGDESAPSGGAEAAAVEPASILLVEDNPGDVKLTKKAFEKAALANHIDVAADGVEAMTYLRDDDRDRPDIVLLDLKLPRMSGHEVLEEIKSDPDLRRIPVVILTSSESERDIVDSYDDHANAYLTKPVTFDGFRDVVKRIEGFWFCVATLPPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETI6</accession>
    <name>A0A1U7ETI6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">P-type transport ATPase (Probable substrate zinc/cadmium)</fullName>
            <ecNumber evidence="12">3.6.3.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">cadA</name>
        <name evidence="12" type="synonym">tpa02</name>
        <name evidence="12" type="ordered locus">NP_0258A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.-" evidence="12"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48220.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_148215418.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETI6"/>
    <dbReference type="STRING" id="348780.NP_0258A"/>
    <dbReference type="EnsemblBacteria" id="CAI48220">
        <property type="protein sequence ID" value="CAI48220"/>
        <property type="gene ID" value="NP_0258A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703349"/>
    <dbReference type="KEGG" id="nph:NP_0258A"/>
    <dbReference type="eggNOG" id="arCOG01576">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001771_6_4_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled monoatomic cation transmembrane transporter activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000002">
        <property type="entry name" value="Copper-transporting ATPase 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
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    </dbReference>
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        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
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        <property type="entry name" value="HAD superfamily/HAD-like"/>
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        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
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        <property type="entry name" value="ATPase_P-typ_P_site"/>
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        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
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        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
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        <property type="entry name" value="ATPase_P-type_domA"/>
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    <dbReference type="InterPro" id="IPR051014">
        <property type="entry name" value="Cation_Transport_ATPase_IB"/>
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        <property type="entry name" value="HAD-like_sf"/>
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    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
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    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
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    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
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    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01512">
        <property type="entry name" value="ATPase-IB2_Cd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48085">
        <property type="entry name" value="CADMIUM/ZINC-TRANSPORTING ATPASE HMA2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48085:SF5">
        <property type="entry name" value="CADMIUM_ZINC-TRANSPORTING ATPASE HMA4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00941">
        <property type="entry name" value="CDATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="88"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="322"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="352"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="698"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="11">
        <location>
            <begin position="4"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI48220.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="742" mass="77107" checksum="CFDF155780A561CE" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETN0</accession>
    <name>A0A1U7ETN0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">hydroxymethylglutaryl-CoA reductase (NADPH)</fullName>
            <ecNumber evidence="2">1.1.1.34</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">hmgA2</name>
        <name evidence="7" type="synonym">mvaA2</name>
        <name evidence="7" type="ordered locus">NP_0368A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(R)-mevalonate + 2 NADP(+) + CoA = (3S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15989"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36464"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.34"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HMG-CoA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.34" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321913.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETN0"/>
    <dbReference type="STRING" id="348780.NP_0368A"/>
    <dbReference type="EnsemblBacteria" id="CAI48275">
        <property type="protein sequence ID" value="CAI48275"/>
        <property type="gene ID" value="NP_0368A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702956"/>
    <dbReference type="KEGG" id="nph:NP_0368A"/>
    <dbReference type="eggNOG" id="arCOG04260">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG05512">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001734_2_2_2"/>
    <dbReference type="OrthoDB" id="10981at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004420">
        <property type="term" value="F:hydroxymethylglutaryl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015936">
        <property type="term" value="P:coenzyme A metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00643">
        <property type="entry name" value="HMG-CoA_reductase_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.770.10">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl-coenzyme A Reductase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.420">
        <property type="entry name" value="Hydroxymethylglutaryl-CoA reductase, class I/II, NAD/NADP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057868">
        <property type="entry name" value="HMG-CoA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002202">
        <property type="entry name" value="HMG_CoA_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023074">
        <property type="entry name" value="HMG_CoA_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004554">
        <property type="entry name" value="HMG_CoA_Rdtase_eu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009023">
        <property type="entry name" value="HMG_CoA_Rdtase_NAD(P)-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009029">
        <property type="entry name" value="HMG_CoA_Rdtase_sub-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572:SF24">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00368">
        <property type="entry name" value="HMG-CoA_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25653">
        <property type="entry name" value="HMG-CoA_red_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00071">
        <property type="entry name" value="HMGCOARDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55035">
        <property type="entry name" value="NAD-binding domain of HMG-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56542">
        <property type="entry name" value="Substrate-binding domain of HMG-CoA reductase"/>
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    </dbReference>
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        <property type="entry name" value="HMG_COA_REDUCTASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydroxymethylglutaryl-CoA reductase-like" evidence="6">
        <location>
            <begin position="33"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="539" mass="58473" checksum="38DBB70ABD00FDA0" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETN6</accession>
    <name>A0A1U7ETN6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sugar nucleotidyltransferase</fullName>
            <ecNumber evidence="3">2.7.7.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">graD7</name>
        <name evidence="3" type="ordered locus">NP_0384A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.-" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321921.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETN6"/>
    <dbReference type="STRING" id="348780.NP_0384A"/>
    <dbReference type="EnsemblBacteria" id="CAI48283">
        <property type="protein sequence ID" value="CAI48283"/>
        <property type="gene ID" value="NP_0384A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702776"/>
    <dbReference type="KEGG" id="nph:NP_0384A"/>
    <dbReference type="eggNOG" id="arCOG00666">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_4_0_2"/>
    <dbReference type="OrthoDB" id="15372at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04181">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056818">
        <property type="entry name" value="GlmU/GlgC-like_hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42883">
        <property type="entry name" value="GLUCOSE-1-PHOSPHATE THYMIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42883:SF2">
        <property type="entry name" value="THYMIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24894">
        <property type="entry name" value="Hexapep_GlmU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-1-phosphate adenylyltransferase/Bifunctional protein GlmU-like C-terminal hexapeptide" evidence="2">
        <location>
            <begin position="244"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="322" mass="35360" checksum="3AB03ED04D5E786E" modified="2017-05-10" version="1">MKAVVLAGGYATRLWPITKHRPKMLLPVGETTVIDRIFSALEADERIEEVYVSTNERFADAFEQHLADQSFEKPVVSVEETTEEAEKFGVVGALGQLVEREGIDDDLLVVAGDNLIGFDLSEFIDYFQQKETAVLAAYDVGDREKAKSYGLVELDGDRVVDFQEKPAEPNSTLVSIACYAFPQESIRFEEYLSGGNNPDEPGWFIQWLQNQEPVSAFVFDEPWFDIGTPESYLEAVAWTLGGDSVIAESATVERSTIGENVQVMAGAEIADATLDRSVVFEDAVVEGCEIHETIIDEGTHLEGIDLAGALIGAHTQLTNGVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETT3</accession>
    <name>A0A1U7ETT3_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA polymerase II small subunit</fullName>
            <shortName evidence="15">Pol II</shortName>
            <ecNumber evidence="15">2.7.7.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Exodeoxyribonuclease small subunit</fullName>
            <ecNumber evidence="15">3.1.11.1</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">polD1</name>
        <name evidence="15" type="synonym">polB</name>
        <name evidence="18" type="ordered locus">NP_0482A</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 15">Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3' to 5' direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 15">Heterodimer of a large subunit and a small subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the DNA polymerase delta/II small subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="15"/>
    <dbReference type="EC" id="3.1.11.1" evidence="15"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321968.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETT3"/>
    <dbReference type="STRING" id="348780.NP_0482A"/>
    <dbReference type="EnsemblBacteria" id="CAI48332">
        <property type="protein sequence ID" value="CAI48332"/>
        <property type="gene ID" value="NP_0482A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703040"/>
    <dbReference type="KEGG" id="nph:NP_0482A"/>
    <dbReference type="eggNOG" id="arCOG04455">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027850_0_0_2"/>
    <dbReference type="OrthoDB" id="372039at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042575">
        <property type="term" value="C:DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008310">
        <property type="term" value="F:single-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07386">
        <property type="entry name" value="MPP_DNA_pol_II_small_archeal_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04490">
        <property type="entry name" value="PolII_SU_OBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.50:FF:000003">
        <property type="entry name" value="DNA polymerase II small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00325">
        <property type="entry name" value="DNApol_II_A_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007185">
        <property type="entry name" value="DNA_pol_a/d/e_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024826">
        <property type="entry name" value="DNA_pol_delta/II_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011149">
        <property type="entry name" value="Pol2_small_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003116">
        <property type="entry name" value="PRK04036.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003118">
        <property type="entry name" value="PRK04036.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10416">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10416:SF0">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04042">
        <property type="entry name" value="DNA_pol_E_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000803">
        <property type="entry name" value="Arc_Pol2_small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0235">DNA replication</keyword>
    <keyword evidence="11 15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 15 18" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="9 15 18" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="8 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7 15" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5 15 18" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15 18" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA polymerase alpha/delta/epsilon subunit B" evidence="17">
        <location>
            <begin position="331"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="61"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="74"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="87"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="119"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="173"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAI48332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="583" mass="62689" checksum="71BAEE113E3D862D" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETV2</accession>
    <name>A0A1U7ETV2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TRAP-type transport system protein small subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tp53b</name>
        <name evidence="1" type="ordered locus">NP_0542A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321998.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETV2"/>
    <dbReference type="STRING" id="348780.NP_0542A"/>
    <dbReference type="EnsemblBacteria" id="CAI48362">
        <property type="protein sequence ID" value="CAI48362"/>
        <property type="gene ID" value="NP_0542A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703340"/>
    <dbReference type="KEGG" id="nph:NP_0542A"/>
    <dbReference type="eggNOG" id="arCOG02990">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110564_0_0_2"/>
    <dbReference type="OrthoDB" id="212141at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015001">
        <property type="entry name" value="DUF1850"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014451">
        <property type="entry name" value="UCP008455"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08905">
        <property type="entry name" value="DUF1850"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008455">
        <property type="entry name" value="UCP008455"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="162" mass="17371" checksum="49B25E9E74E1B55F" modified="2017-05-10" version="1">MPRRAWLPIAALSVLVVASAAGIVVSERDVGTQTLVVEDATDGTELLAVAVEPGSEVAIEYTHSVERTPVVDVYTVDDGELVMTRSEFQSFGAGLPSRADVALEDGVFVYEPPPKRYAQLTVATGHIADHDLVVDGERYDLAALADGESITLDLEHRLFHGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETX5</accession>
    <name>A0A1U7ETX5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ribose-phosphate diphosphokinase</fullName>
            <ecNumber evidence="1">2.7.6.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">prsA</name>
        <name evidence="11" type="ordered locus">NP_0606A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 13">
            <text>D-ribose 5-phosphate + ATP = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the ribose-phosphate pyrophosphokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322030.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETX5"/>
    <dbReference type="STRING" id="348780.NP_0606A"/>
    <dbReference type="EnsemblBacteria" id="CAI48394">
        <property type="protein sequence ID" value="CAI48394"/>
        <property type="gene ID" value="NP_0606A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703059"/>
    <dbReference type="KEGG" id="nph:NP_0606A"/>
    <dbReference type="eggNOG" id="arCOG00067">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033546_2_2_2"/>
    <dbReference type="OrthoDB" id="371997at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002189">
        <property type="term" value="C:ribose phosphate diphosphokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210:SF32">
        <property type="entry name" value="RIBOSE-PHOSPHATE PYROPHOSPHOKINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01400">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 8" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribose-phosphate pyrophosphokinase N-terminal" evidence="10">
        <location>
            <begin position="2"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="9">
        <location>
            <begin position="150"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI48394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="277" mass="29053" checksum="9D5ADC853F89FE1E" modified="2017-05-10" version="1">MIVPGSDTQELAAALSAALDEPLAAVEYDRFADGERLVQVPETDDRAVVVASTVSDSAHVELLQLQDAARQQAEEVVTVLPYMGYARQDAAFEDGQPVSARAIARAVATGTDRVLTVNPHETAVCDFFDVPATPVDAAGRLAEPLPSLSDPLFLSPDAGAIGLAETVSDAYGDGEVDYFEKTRTSDTEVEIEPSDATTTGRDIVLVDDIVATGSTMSTAVAQLTAPARVFVTCVHPMLAANARTKLERAGVDAVYGTDTIERPVSAVSVAPAIAEHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETY4</accession>
    <name>A0A1U7ETY4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Origin-associated GTP-binding protein OapA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">oapA</name>
        <name evidence="2" type="ordered locus">NP_0594A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322024.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETY4"/>
    <dbReference type="STRING" id="348780.NP_0594A"/>
    <dbReference type="EnsemblBacteria" id="CAI48388">
        <property type="protein sequence ID" value="CAI48388"/>
        <property type="gene ID" value="NP_0594A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700900"/>
    <dbReference type="KEGG" id="nph:NP_0594A"/>
    <dbReference type="eggNOG" id="arCOG00354">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1399745_0_0_2"/>
    <dbReference type="OrthoDB" id="311119at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00880">
        <property type="entry name" value="Era_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42714">
        <property type="entry name" value="TRNA MODIFICATION GTPASE GTPBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42714:SF2">
        <property type="entry name" value="TRNA MODIFICATION GTPASE GTPBP3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="1">
        <location>
            <begin position="26"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="213" mass="23715" checksum="965E310C0FBB6C94" modified="2017-05-10" version="1">MGLLTDLKSSISRATSSLFAGDETKRIGIYGPPNAGKTTLANRITRDWTGDAVGPESHVPHETRRARRKENVEIKRNGKSVTIDVVDTPGVTTKVDYKEFLDHDIDKDDAVRRSREATEGVAEAMHWLREDVDGVIYVLDATEDPFTQVNTMLIGIIESQDLPVLIFANKIDLEDANVQRISNAFPQHETVPLSALEGDNMDEVYDKIAEYFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU02</accession>
    <name>A0A1U7EU02_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UspA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0658A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322056.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU02"/>
    <dbReference type="STRING" id="348780.NP_0658A"/>
    <dbReference type="EnsemblBacteria" id="CAI48420">
        <property type="protein sequence ID" value="CAI48420"/>
        <property type="gene ID" value="NP_0658A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700919"/>
    <dbReference type="KEGG" id="nph:NP_0658A"/>
    <dbReference type="eggNOG" id="arCOG02053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_11_4_2"/>
    <dbReference type="OrthoDB" id="105697at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF24">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="2"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="139" mass="14337" checksum="1C8BEAE0402F6B1B" modified="2017-05-10" version="1">MHVLVPVDGSGPSDDALAYALDQFGSAEITAVYVMDPVDGATTWGPGSGDDWLSAAESRAEEVLESAREQATAADTAVTTASVVGRPARAIVDYAEDNDIDHIVVGSHGRSGVSRMLLGSVAETVVRRSPVPVTVARSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU22</accession>
    <name>A0A1U7EU22_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF4393 domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0718A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322086.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU22"/>
    <dbReference type="STRING" id="348780.NP_0718A"/>
    <dbReference type="EnsemblBacteria" id="CAI48450">
        <property type="protein sequence ID" value="CAI48450"/>
        <property type="gene ID" value="NP_0718A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700941"/>
    <dbReference type="KEGG" id="nph:NP_0718A"/>
    <dbReference type="HOGENOM" id="CLU_637135_0_0_2"/>
    <dbReference type="OrthoDB" id="234612at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025506">
        <property type="entry name" value="Abi_alpha"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14337">
        <property type="entry name" value="Abi_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="456" mass="48449" checksum="34F3E11744B496EB" modified="2017-05-10" version="1">MTDDQPSDDDPSSSPPPDDHPNSGPPADDGRDSDLPSGDGPSATPTASPSDLERALSVLETAVESERVGESRLERLLAALERVVATPAGADPETVAEFLSLLEGALADAGRLNEVDFDGLLSVLETAAAAVPSVDETAAEEVLGVLERGLRDPNSLEQTDIETLNEQIERLVAGATDPTTGGIDRLFPESGPTEGVTNAFRIAHIVTAMTQRATGHAVESGLRTGTRMGYAAATAESPAALLTEARAVALDELRRSGVDIGDERQAWLEAHEDALIADRPVTEEQLRARGERLLSKSATLGRPEGPHPAFGAIIDQLAEDEARILRLLATDGAQPTVDIYERGYIPFNARLVAAALTKLGSDAGCRTPKRVPLYVQNLDRLGLVTLSEEPVENLKIYQVLEAQPHVEQARQGVTRPKTDYGSIRLTELGAAFCEACFPVDVADSPPATELRRDGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU23</accession>
    <name>A0A1U7EU23_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DUF2078 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0692A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322073.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU23"/>
    <dbReference type="STRING" id="348780.NP_0692A"/>
    <dbReference type="EnsemblBacteria" id="CAI48437">
        <property type="protein sequence ID" value="CAI48437"/>
        <property type="gene ID" value="NP_0692A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700932"/>
    <dbReference type="KEGG" id="nph:NP_0692A"/>
    <dbReference type="eggNOG" id="arCOG03909">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155662_0_0_2"/>
    <dbReference type="OrthoDB" id="242726at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018649">
        <property type="entry name" value="SHOCT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09851">
        <property type="entry name" value="SHOCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="SHOCT" evidence="2">
        <location>
            <begin position="82"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="111" mass="12056" checksum="A3D40EA33674D36A" modified="2017-05-10" version="1">MATNDTLLRAVLVVIVAVLAVPLVMMTVAMPMAGVMHGGMWGNGTGMGGLWWLWSLLPLAVLIALGYGGYRLLVADGSDDSAMQELRTAYARGDLTDEEFETRRQRLKNSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU30</accession>
    <name>A0A1U7EU30_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PHP domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0722A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322088.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU30"/>
    <dbReference type="STRING" id="348780.NP_0722A"/>
    <dbReference type="DNASU" id="3700943"/>
    <dbReference type="EnsemblBacteria" id="CAI48452">
        <property type="protein sequence ID" value="CAI48452"/>
        <property type="gene ID" value="NP_0722A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700943"/>
    <dbReference type="KEGG" id="nph:NP_0722A"/>
    <dbReference type="eggNOG" id="arCOG00302">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067347_1_0_2"/>
    <dbReference type="OrthoDB" id="196608at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004013">
        <property type="entry name" value="PHP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052018">
        <property type="entry name" value="PHP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003141">
        <property type="entry name" value="Pol/His_phosphatase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42924">
        <property type="entry name" value="EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42924:SF18">
        <property type="entry name" value="POLYMERASE_HISTIDINOL PHOSPHATASE N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02811">
        <property type="entry name" value="PHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00481">
        <property type="entry name" value="POLIIIAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="PHP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polymerase/histidinol phosphatase N-terminal" evidence="1">
        <location>
            <begin position="4"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="256" mass="27623" checksum="5E80B848FCF1EA01" modified="2017-05-10" version="1">MVVADLHVHTQNSDGSLTLGAIPEAAKAAGLSTVAVTDHDRLHPEMRTPVAELDGITVVHGIELRVEADDQRVDLLGYGVRRTEALSALVDRLQTDRKERGAAIIDRVEAELGTELPVEPRAGLGRPHIAAAIEQVSDYSYGAAFEELIGDGRPCYVARDVPDFETGVSVLDAACGVVSLAHPHRYADTEAALSLCADLDAVERHYPYDRAVDHRPVERAIERYDLLVTGGTDAHGETLGVAGLDGRDYRTFRTAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU41</accession>
    <name>A0A1U7EU41_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">NP_0730A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322092.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU41"/>
    <dbReference type="STRING" id="348780.NP_0730A"/>
    <dbReference type="EnsemblBacteria" id="CAI48456">
        <property type="protein sequence ID" value="CAI48456"/>
        <property type="gene ID" value="NP_0730A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700947"/>
    <dbReference type="KEGG" id="nph:NP_0730A"/>
    <dbReference type="eggNOG" id="arCOG02332">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_58_2"/>
    <dbReference type="OrthoDB" id="8127at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050736">
        <property type="entry name" value="Sensor_HK_Regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43711:SF1">
        <property type="entry name" value="HISTIDINE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43711">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Histidine kinase" evidence="8">
        <location>
            <begin position="310"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="338"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI48456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="504" mass="54546" checksum="5952630AA693D1CE" modified="2017-05-10" version="1">MQSRDGQGMQSSAASATVLCAVADGAEPVASALDDGFRVRTLGPDSDVLDVLDGGDIDCLVADEHVLSNIEAISEHVGHVIVFSGSVAPRLSQAAARRSGWDVVYRDAAPVETPGTEVDRLHERLETMFQGSTEDIGDTVLEVARLLMSAAPDEVDTRIEWGLESLGEALDADRCVLYAYGEDDRFSRVSGWQRTGAASPPDAVPVEQFPDADTLLQFSTVSVAAPPSEPPAAEYRSRHDIGTFVAVPVVIDWELEYVLAACRSGTEPWPASVQRQLRTAGELVGHTLRRHRRRNEIERKNERLERFASVISHDLQNPLNVILGYADLAMESRDPDDIERIATAAERMETILEELQTLVQESRALGETEPVAISTVVERARSSVAMPKATVDIEEIGVAEADPGRLRQAFENLLRNAVEHAGPDVTVRVVPINGGFAVEDDGPGIHPDKREAVFEEGHTDGDGTGLGLSIVRTVVEAHGWSVTATDAEDGGARFEVTDVDFHEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU54</accession>
    <name>A0A1U7EU54_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC-type transport system ATP-binding protein (Probable substrate dipeptide/oligopeptide)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">dppD1</name>
        <name evidence="10" type="synonym">abc01a1</name>
        <name evidence="10" type="ordered locus">NP_0764A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322109.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU54"/>
    <dbReference type="STRING" id="348780.NP_0764A"/>
    <dbReference type="EnsemblBacteria" id="CAI48473">
        <property type="protein sequence ID" value="CAI48473"/>
        <property type="gene ID" value="NP_0764A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702362"/>
    <dbReference type="KEGG" id="nph:NP_0764A"/>
    <dbReference type="eggNOG" id="arCOG00181">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_2"/>
    <dbReference type="OrthoDB" id="18209at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03257">
        <property type="entry name" value="ABC_NikE_OppD_transporters"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050388">
        <property type="entry name" value="ABC_Ni/Peptide_Import"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43297:SF14">
        <property type="entry name" value="ATPASE AAA-TYPE CORE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43297">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORT ATP-BINDING PROTEIN APPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="6"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI48473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="388" mass="42226" checksum="766BEFB41A7A9795" modified="2017-05-10" version="1">MSETKLDVRDLQTRFYTDDGQVNAVDGVSFTVESGEVFGIVGESGSGKSVTALSLIDLVESPGRIDGGEIWFRSPEMADRLADDSPAAVDGEMVDIRQLDDRERRSLRGAEFATIFQDPMSSFNPSLTVGEQIAEAVEAQKRAQANPRSTRSRTQGYGFASFVADSLLPRRDYVSEDATTRAVELLELVGIPDPADRADEYPHQFSGGMLQRAMIAQALASEPSVLVADEPTTALDVTIQAQVLDLLADLQAETGMTTVLITHNLGVVARMCDRVGVMYAGELVERGTLGEVFDESVHPYTEGLLGSIPDLDGESGRLSPIEGNVPSLIDSEMESRCYFADRCPKAMEECLDKPPEYEVTDDHTAMCYLADHDYDPGNALPEGYFDAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU57</accession>
    <name>A0A1U7EU57_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glutamine amidotransferase (Homolog to GMP synthase subunit A)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">guaAa2</name>
        <name evidence="2" type="ordered locus">NP_0794A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322124.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU57"/>
    <dbReference type="STRING" id="348780.NP_0794A"/>
    <dbReference type="EnsemblBacteria" id="CAI48488">
        <property type="protein sequence ID" value="CAI48488"/>
        <property type="gene ID" value="NP_0794A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702781"/>
    <dbReference type="KEGG" id="nph:NP_0794A"/>
    <dbReference type="eggNOG" id="arCOG00090">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054974_4_1_2"/>
    <dbReference type="OrthoDB" id="7388at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01741">
        <property type="entry name" value="GATase1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044992">
        <property type="entry name" value="ChyE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42695">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE YLR126C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42695:SF5">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE YLR126C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="1">
        <location>
            <begin position="42"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="234" mass="26008" checksum="463D876DB59A1031" modified="2017-05-10" version="1">MRPRIALLDASHNDPTTPRNFRRELDATVERFSVVDGEFPSTRRFDGLVVSGSRASVYWDEPWITATREWVRNAVADGLSALGICWGHQLLADALGGTVEPMGEYELGYRTVSHGGAGLFDGVPEEFTVFTTHSDAVTELPPGAELIATNDYGVHGFRHGNVYGLQSHPEYDPETAESVVRGKDHLPETRIERVCEEITPAAYERAQPAKRAFDNFLQVVERNRTRPTPQVADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU73</accession>
    <name>A0A1U7EU73_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">anthranilate synthase</fullName>
            <ecNumber evidence="3">4.1.3.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pabB</name>
        <name evidence="9" type="synonym">trpE2</name>
        <name evidence="9" type="ordered locus">NP_0802A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21732"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="4.1.3.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the anthranilate synthase component I family.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.27" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322128.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU73"/>
    <dbReference type="STRING" id="348780.NP_0802A"/>
    <dbReference type="EnsemblBacteria" id="CAI48492">
        <property type="protein sequence ID" value="CAI48492"/>
        <property type="gene ID" value="NP_0802A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703415"/>
    <dbReference type="KEGG" id="nph:NP_0802A"/>
    <dbReference type="eggNOG" id="arCOG02014">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006493_9_3_2"/>
    <dbReference type="OrthoDB" id="25514at2157"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00040"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004049">
        <property type="term" value="F:anthranilate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.120.10">
        <property type="entry name" value="Anthranilate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005801">
        <property type="entry name" value="ADC_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019999">
        <property type="entry name" value="Anth_synth_I-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006805">
        <property type="entry name" value="Anth_synth_I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015890">
        <property type="entry name" value="Chorismate_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236">
        <property type="entry name" value="AMINOBENZOATE/ANTHRANILATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236:SF9">
        <property type="entry name" value="ANTHRANILATE SYNTHASE COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04715">
        <property type="entry name" value="Anth_synt_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00425">
        <property type="entry name" value="Chorismate_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00095">
        <property type="entry name" value="ANTSNTHASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56322">
        <property type="entry name" value="ADC synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Anthranilate synthase component I N-terminal" evidence="8">
        <location>
            <begin position="32"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Chorismate-utilising enzyme C-terminal" evidence="7">
        <location>
            <begin position="218"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI48492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="496" mass="54022" checksum="8EE7449A3A1317B8" modified="2017-05-10" version="1">MRVVTDRASFRSAAADAPDSARVPVELRVTVADPFEAYRRARDGDGGDIYLETTGGQSGWGYFATDPRERTTVRASAVASDGESPSLEAVDSLLEGTTLERGGCDVPYPCGVFGWLSYDIARELESLPDDTRDDRGLPRLQVATYDRVAAWEEPRSEETTLRVTACPKVGAYDSMSAAYEHGVERARDLIAKATNGDPSVGQPPVSGTEATFESDCTRAAFADRVRRVKSYIRDGETFQANISQRLSAPATVHPVSAYAALRRVNPAPYSALVEFPGVDLVSASPELLLDVDGDRLLTEPIAGTRPRGETQTEDERLEADLLGDEKERAEHAMLVDLERNDLGKVSEYGSVDVREYRRVDRYSEVMHLVSLAEGTRRADCSLADAVGAVFPGGTITGAPKPRTMEIIDEVETTRRGPYTGAIGLFGFDERATLNIVIRTLVRQGDSYYLRVGAGIVDDSDPDAEYDETLDKGRALVTAIDEALAERATFTMEAAKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUB6</accession>
    <name>A0A1U7EUB6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0932A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322193.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUB6"/>
    <dbReference type="STRING" id="348780.NP_0932A"/>
    <dbReference type="EnsemblBacteria" id="CAI48557">
        <property type="protein sequence ID" value="CAI48557"/>
        <property type="gene ID" value="NP_0932A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701003"/>
    <dbReference type="KEGG" id="nph:NP_0932A"/>
    <dbReference type="eggNOG" id="arCOG08183">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2695879_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055955">
        <property type="entry name" value="DUF7533"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24377">
        <property type="entry name" value="DUF7533"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="76" mass="8138" checksum="7B58BF8244DAE3D1" modified="2017-05-10" version="1">MTLGIVRTLQLAATLVVAGPVGLVGVFNLIEGNHLHGVAFLAAAVAIVAVSEYVYLRLTDRTVGRLRRIGGRLRRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUC2</accession>
    <name>A0A1U7EUC2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Probable oxidoreductase (Short-chain dehydrogenase family)</fullName>
            <ecNumber evidence="3">1.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0892A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322173.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUC2"/>
    <dbReference type="STRING" id="348780.NP_0892A"/>
    <dbReference type="EnsemblBacteria" id="CAI48537">
        <property type="protein sequence ID" value="CAI48537"/>
        <property type="gene ID" value="NP_0892A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700990"/>
    <dbReference type="KEGG" id="nph:NP_0892A"/>
    <dbReference type="eggNOG" id="arCOG01263">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_1_2"/>
    <dbReference type="OrthoDB" id="7442at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44196">
        <property type="entry name" value="DEHYDROGENASE/REDUCTASE SDR FAMILY MEMBER 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44196:SF3">
        <property type="entry name" value="SHORT CHAIN DEHYDROGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="233" mass="24632" checksum="A773A834DB925BA6" modified="2017-05-10" version="1">MPTAVIVGASSGIGRALAEELADDYELGLAARRLDELRAVGESIGGAHVAAIDVTEPNARERFDALADSLDGVDVVVISAGIARYNDALEWDPERETVAVNAAGFTAIATAAIDHFEATGGGQLVGISSVAAEVGSPTLPAYSASKAYVSRYLEGLRYRAGEDIAVTDVRPGFVDTDMSPDADRFWECSPETAAAGIARAIEREPAVAYVPRRWWLVSKLLGVLPDRLRAEAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUI1</accession>
    <name>A0A1U7EUI1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7967 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1084A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322269.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUI1"/>
    <dbReference type="STRING" id="348780.NP_1084A"/>
    <dbReference type="EnsemblBacteria" id="CAI48633">
        <property type="protein sequence ID" value="CAI48633"/>
        <property type="gene ID" value="NP_1084A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701049"/>
    <dbReference type="KEGG" id="nph:NP_1084A"/>
    <dbReference type="eggNOG" id="arCOG03917">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153002_0_0_2"/>
    <dbReference type="OrthoDB" id="156620at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058273">
        <property type="entry name" value="DUF7967"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25921">
        <property type="entry name" value="DUF7967"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7967" evidence="1">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="87" mass="10141" checksum="914BA520AC91FE40" modified="2017-05-10" version="1">MTDTERLWLVEREYGDESLVELVYATPDGSRCRVKHLSVQLLRKKDVTAAIEVDPEKLDAVRDEATQQRYAEEVQRVRENHDPDETV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUM5</accession>
    <name>A0A1U7EUM5_NATPD</name>
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        <submittedName>
            <fullName evidence="7">Thermosome subunit 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ths3</name>
        <name evidence="7" type="ordered locus">NP_1182A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUM5"/>
    <dbReference type="STRING" id="348780.NP_1182A"/>
    <dbReference type="EnsemblBacteria" id="CAI48682">
        <property type="protein sequence ID" value="CAI48682"/>
        <property type="gene ID" value="NP_1182A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703259"/>
    <dbReference type="KEGG" id="nph:NP_1182A"/>
    <dbReference type="eggNOG" id="arCOG01257">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008891_7_3_2"/>
    <dbReference type="OrthoDB" id="9362at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Chaperone_TCP-1"/>
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        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="526" mass="54861" checksum="6419FE9DE0052B46" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUM7</accession>
    <name>A0A1U7EUM7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Probable oxidoreductase (Short-chain dehydrogenase family)</fullName>
            <ecNumber evidence="4">1.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_1186A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322320.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUM7"/>
    <dbReference type="STRING" id="348780.NP_1186A"/>
    <dbReference type="EnsemblBacteria" id="CAI48684">
        <property type="protein sequence ID" value="CAI48684"/>
        <property type="gene ID" value="NP_1186A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701078"/>
    <dbReference type="KEGG" id="nph:NP_1186A"/>
    <dbReference type="eggNOG" id="arCOG01259">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_10_2"/>
    <dbReference type="OrthoDB" id="161871at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketoreductase" evidence="3">
        <location>
            <begin position="7"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="240" mass="25161" checksum="E8A8EB4C7D54276B" modified="2017-05-10" version="1">MGRLDDETVIVTGASRGLGASMAKRFAREGANTVLAARSEDQLAAVADETDGETLVCPTDVTDEASVAALVDATVDAYGDLTGLVNNAAVGLLSLYDERRQTTDIDADDWRLIMEVNATGPFLCSKHAVPEMEAGNIINISSGLGRRGEAGWAPYVASKWALEGFTRTLADEVAPEINVNALDPGGRAKTGFWDHLSADEQASILPADVMDDAAVMLLAQGPGGVTAASLPAQDWEDRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUN4</accession>
    <name>A0A1U7EUN4_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7119 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_1152A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322303.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUN4"/>
    <dbReference type="STRING" id="348780.NP_1152A"/>
    <dbReference type="EnsemblBacteria" id="CAI48667">
        <property type="protein sequence ID" value="CAI48667"/>
        <property type="gene ID" value="NP_1152A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701068"/>
    <dbReference type="KEGG" id="nph:NP_1152A"/>
    <dbReference type="eggNOG" id="arCOG06352">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1197624_0_0_2"/>
    <dbReference type="OrthoDB" id="169823at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2830">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055543">
        <property type="entry name" value="DUF7119"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23433">
        <property type="entry name" value="DUF7119"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109709">
        <property type="entry name" value="KorB DNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7119" evidence="2">
        <location>
            <begin position="37"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="217" mass="23397" checksum="30E79A7A40693817" modified="2017-05-10" version="1">MTPQPDGEPPTDRESPVGEPVIRSDPRVAGEQAVQFDPDDPESLNEAADVVRRFATNTAGSKDNVFMLRGAAACAALVRGTGSYKAAAERAGGEATVSFIRKWARVHDLPRSIRRHVALGEIAPTAAKHIARVSGESRFLLAWATLDHDLTVREVRAIASRVNEGASVDEALAAEGIDLGQLSVSLPPETYRELRRHAALEETDPDEIVARALAEYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUP2</accession>
    <name>A0A1U7EUP2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4083 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1242A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322348.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUP2"/>
    <dbReference type="EnsemblBacteria" id="CAI48712">
        <property type="protein sequence ID" value="CAI48712"/>
        <property type="gene ID" value="NP_1242A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701093"/>
    <dbReference type="KEGG" id="nph:NP_1242A"/>
    <dbReference type="HOGENOM" id="CLU_2911622_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="61" mass="6955" checksum="0F4FBC1D0EC2C9FA" modified="2017-05-10" version="1">MLPLQMSFSGAELFVLFLILLLLLAPIAVAVAVLVYLRSKRNDRLEALEERIERLEAEQDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUP9</accession>
    <name>A0A1U7EUP9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PhzF family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">phzF</name>
        <name evidence="2" type="ordered locus">NP_1202A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322328.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUP9"/>
    <dbReference type="STRING" id="348780.NP_1202A"/>
    <dbReference type="EnsemblBacteria" id="CAI48692">
        <property type="protein sequence ID" value="CAI48692"/>
        <property type="gene ID" value="NP_1202A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703027"/>
    <dbReference type="KEGG" id="nph:NP_1202A"/>
    <dbReference type="eggNOG" id="arCOG02256">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048756_0_2_2"/>
    <dbReference type="OrthoDB" id="105902at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.10">
        <property type="entry name" value="Diaminopimelate Epimerase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003719">
        <property type="entry name" value="Phenazine_PhzF-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00654">
        <property type="entry name" value="PhzF_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13774:SF39">
        <property type="entry name" value="BIOSYNTHESIS PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13774">
        <property type="entry name" value="PHENAZINE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02567">
        <property type="entry name" value="PhzC-PhzF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016184">
        <property type="entry name" value="PhzC_PhzF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54506">
        <property type="entry name" value="Diaminopimelate epimerase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="300" mass="31013" checksum="A403F56BDC2C2F05" modified="2017-05-10" version="1">METRRALLVDAFTTDPLAGNPAGVVPDADGLADDQLQAIAAELGASETAFVLESDDADHRLRFFTPTTEVDLCGHATVAAHAWLAETGRLDDGSYTVETDAGAVAVEIDSGTVWLSQRPAEVAAVELDYERLATALGADPATLTDVGADMPLATASTGLPYLVVPVNFLEGLSALDPNADAVASLSDELDVAGVYAFTFDTLGAETTLHARMFAPALGVDEDPVTGTAAAAVGAYLRAFEAFDGELPAEVVVEQGHFIDRPGKVRVQAQADPVTVGGEAATAFDGELSVPEPGDDDIIEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUQ1</accession>
    <name>A0A1U7EUQ1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DoxX domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">NP_1248A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322351.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUQ1"/>
    <dbReference type="STRING" id="348780.NP_1248A"/>
    <dbReference type="DNASU" id="3701094"/>
    <dbReference type="EnsemblBacteria" id="CAI48715">
        <property type="protein sequence ID" value="CAI48715"/>
        <property type="gene ID" value="NP_1248A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701094"/>
    <dbReference type="KEGG" id="nph:NP_1248A"/>
    <dbReference type="eggNOG" id="arCOG03070">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058421_8_2_2"/>
    <dbReference type="OrthoDB" id="340328at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032808">
        <property type="entry name" value="DoxX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07681">
        <property type="entry name" value="DoxX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="105"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI48715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="126" mass="13530" checksum="23EF71BBEC160826" modified="2017-05-10" version="1">MVDDNTDQSLSFGRLLFGAGLALQASEDFREMDETVEYAESAGVPVPDVLAPLASGMMLVGGIGVALWRFPRIATGAVVTFLAVVTPTMHDFWNAEPGDRSGERLAFFGNLAMLGAALAYLRKAYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUZ6</accession>
    <name>A0A1U7EUZ6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC-type transport system periplasmic substrate-binding protein (Probable substrate branched-chain amino acids)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">livJ3</name>
        <name evidence="3" type="synonym">abc07s</name>
        <name evidence="3" type="ordered locus">NP_1464A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUZ6"/>
    <dbReference type="STRING" id="348780.NP_1464A"/>
    <dbReference type="EnsemblBacteria" id="CAI48823">
        <property type="protein sequence ID" value="CAI48823"/>
        <property type="gene ID" value="NP_1464A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_1464A"/>
    <dbReference type="eggNOG" id="arCOG01020">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027128_0_0_2"/>
    <dbReference type="OrthoDB" id="200499at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06345">
        <property type="entry name" value="PBP1_ABC_ligand_binding-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051010">
        <property type="entry name" value="BCAA_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028081">
        <property type="entry name" value="Leu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30483">
        <property type="entry name" value="LEUCINE-SPECIFIC-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30483:SF6">
        <property type="entry name" value="PERIPLASMIC BINDING PROTEIN OF ABC TRANSPORTER FOR NATURAL AMINO ACIDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13458">
        <property type="entry name" value="Peripla_BP_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Leucine-binding protein" evidence="2">
        <location>
            <begin position="64"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="441" mass="47602" checksum="912AC0D8FCB8A650" modified="2017-05-10" version="1">MVNNCKGDSRNVRSAAGRSVGRRRFLKATGAGAVTLGLAGCADNGGEFDDSTFKVGHLAPLELDMGAGSERSADIAVDELNANGGVMDTDVELIHADTSAIPSEATRQAETLVQDENVDMLIGAHASEVVLAILDFVAESEIPFLVTGSAAPEVSTNYMGDDYDRYEMIFRPGPANSSYQVDELAGYAEYLNDEHGWTTFAQLAEQAAWTQFFTDNLPPALEDRGFDVEYNERISSETDDFSPILDAIEATGADAVFKQFSLLPGTGMLSEWRNSEYPFGQEGVSVPSMSPEYWDDTDGGCQYETTAESGGAGASPITDLTQPFTEEYEAVADGERPTLPMYMGYCTYDGLMLYAEAVERAGTFDYRNNLQDILDELRATDFTGTTGQIVFGEEGSEFPNDVIAGPDGGAGFPVTQWIDGSKEIVYPRDIASADHQAAPWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV23</accession>
    <name>A0A1U7EV23_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Fe-coproporphyrin synthase AhbC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ahbC</name>
        <name evidence="8" type="synonym">cmo2</name>
        <name evidence="8" type="ordered locus">NP_1542A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322496.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV23"/>
    <dbReference type="STRING" id="348780.NP_1542A"/>
    <dbReference type="EnsemblBacteria" id="CAI48862">
        <property type="protein sequence ID" value="CAI48862"/>
        <property type="gene ID" value="NP_1542A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702615"/>
    <dbReference type="KEGG" id="nph:NP_1542A"/>
    <dbReference type="eggNOG" id="arCOG00938">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009273_4_0_2"/>
    <dbReference type="OrthoDB" id="30736at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21123">
        <property type="entry name" value="SPASM_MftC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000325">
        <property type="entry name" value="Radical SAM domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023885">
        <property type="entry name" value="4Fe4S-binding_SPASM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017200">
        <property type="entry name" value="PqqE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027626">
        <property type="entry name" value="Pseudo_SAM_Halo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050377">
        <property type="entry name" value="Radical_SAM_PqqE_MftC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04347">
        <property type="entry name" value="pseudo_SAM_Halo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04085">
        <property type="entry name" value="rSAM_more_4Fe4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228:SF7">
        <property type="entry name" value="PQQA PEPTIDE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228">
        <property type="entry name" value="RADICAL SAM DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13186">
        <property type="entry name" value="SPASM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037420">
        <property type="entry name" value="PQQ_syn_pqqE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01386">
        <property type="entry name" value="main_SPASM_domain-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="34"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI48862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="400" mass="43916" checksum="A668F43C03AD8D53" modified="2017-05-10" version="1">MISVSTLLCDHAADGNQLRYNVDTGTDNPVSEEPQRRPVVVWNTTNRCNLKCVHCYAGAETEAKAGELTTTEGKRLLDDLADYGAPVVLFSGGEPMVREDLTELVAYAADAGIRPVLSTNGTLLTEERARELKEAGLAYAGVSVDGLAERNDEFRGVDGAFQAAVEGIESCLDVGLKTGLRYTVTGQNVDDLDGVVDLLTDVGLDRFCFYHLSYSGRGETLSDIDLDAAERREVVKRICDRTLRSHRNGDEIETLLVGNYADAAYIVEYARREFGDEAAERVYKRLEQNGGDPTGERVAAVDCLGNVHPTQFWRTYSLGNVRDRPFSDIWEDESNPLLRAVRNRPENLTGKCADCQYKEICRGGSRLRALASGNGLFGPDPQCYLDEAERQGTRPPASAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV48</accession>
    <name>A0A1U7EV48_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF2249 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1550A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322500.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV48"/>
    <dbReference type="STRING" id="348780.NP_1550A"/>
    <dbReference type="EnsemblBacteria" id="CAI48866">
        <property type="protein sequence ID" value="CAI48866"/>
        <property type="gene ID" value="NP_1550A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701178"/>
    <dbReference type="KEGG" id="nph:NP_1550A"/>
    <dbReference type="eggNOG" id="arCOG03928">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_146484_1_0_2"/>
    <dbReference type="OrthoDB" id="198577at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018720">
        <property type="entry name" value="DUF2249"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10006">
        <property type="entry name" value="DUF2249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2249" evidence="1">
        <location>
            <begin position="4"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="76" mass="8607" checksum="9E6F5A731950EF9F" modified="2017-05-10" version="1">MGETLDVRDIPPSDRHPKIHAAFEALAAGETLTIVNDHEPKPLFYEFQAEVESFDADGYEVEQASPNEFRAHFPKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV53</accession>
    <name>A0A1U7EV53_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Sirohydrochlorin cobaltochelatase</fullName>
            <ecNumber evidence="5">4.99.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cbiX2</name>
        <name evidence="5" type="ordered locus">NP_1588A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.99.1.3" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322519.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV53"/>
    <dbReference type="STRING" id="348780.NP_1588A"/>
    <dbReference type="EnsemblBacteria" id="CAI48885">
        <property type="protein sequence ID" value="CAI48885"/>
        <property type="gene ID" value="NP_1588A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701194"/>
    <dbReference type="KEGG" id="nph:NP_1588A"/>
    <dbReference type="eggNOG" id="arCOG02246">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096061_0_0_2"/>
    <dbReference type="OrthoDB" id="11653at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016852">
        <property type="term" value="F:sirohydrochlorin cobaltochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03416">
        <property type="entry name" value="CbiX_SirB_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1400">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002762">
        <property type="entry name" value="CbiX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050963">
        <property type="entry name" value="Sirohydro_Cobaltochel/CbiX"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002670">
        <property type="entry name" value="PRK02395.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002671">
        <property type="entry name" value="PRK02395.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33542">
        <property type="entry name" value="SIROHYDROCHLORIN FERROCHELATASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33542:SF3">
        <property type="entry name" value="SIROHYDROCHLORIN FERROCHELATASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01903">
        <property type="entry name" value="CbiX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53800">
        <property type="entry name" value="Chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="4" id="KW-0170">Cobalt</keyword>
    <keyword evidence="3 5" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI48885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="296" mass="32471" checksum="7A6C018BAAFBA922" modified="2017-05-10" version="1">MQALVIVAHGSHLNPDASTPTYDHADTIRETGVFDEVQTAFWKEEPSFREALRTVESDEVYVVPLFISEGYFTEEVIPRELRLDGWETAEWDSDGTSATHATLTAADVDKTVHYCGPVGTHDAMTDVVVRRAETVTDDPGVGDGFGLAVVGHGTERNENSAKAIEYHADRVAEMDRFDEVKALFMDEDPEIDDVTDFFETDDIVVVPLFVADGYHTQEDIPEDMGLTDDYRTGWETPADVDGHHIWYAGAVGTEGLMADVIIERAADAGADVGDAIEEVRRRTKVATTDVNASAGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV73</accession>
    <name>A0A1U7EV73_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HD family hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1636A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322543.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV73"/>
    <dbReference type="STRING" id="348780.NP_1636A"/>
    <dbReference type="EnsemblBacteria" id="CAI48909">
        <property type="protein sequence ID" value="CAI48909"/>
        <property type="gene ID" value="NP_1636A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701207"/>
    <dbReference type="KEGG" id="nph:NP_1636A"/>
    <dbReference type="eggNOG" id="arCOG01860">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036524_1_0_2"/>
    <dbReference type="OrthoDB" id="17914at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006675">
        <property type="entry name" value="HDIG_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00277">
        <property type="entry name" value="HDIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33594:SF1">
        <property type="entry name" value="HD_PDEASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33594">
        <property type="entry name" value="SUPERFAMILY HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G03035)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="50"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="232" mass="26243" checksum="2DC047C68B029236" modified="2017-05-10" version="1">MGVEIRESPVSDEEFEEMKRFVRDYLAASVESEDEGGRMRWYPWHSAEYRFNHILNVTELASKIAAEEGANVDVTRVAALFHDIAKLEAEQDVHAEAGARVAREYLRSRTDYPESFIDQVCAAVADHSYEGDLDTMPLETQCLMEADLLDKVGANGTMLMLLRMGYEARTHMDANEMVGRVLERGEDAIARVRSDTAQSIAHQRIKRVRWFKEWLEGEVAGMDPEDGAAAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVA3</accession>
    <name>A0A1U7EVA3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1688A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322568.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVA3"/>
    <dbReference type="STRING" id="348780.NP_1688A"/>
    <dbReference type="EnsemblBacteria" id="CAI48935">
        <property type="protein sequence ID" value="CAI48935"/>
        <property type="gene ID" value="NP_1688A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701224"/>
    <dbReference type="KEGG" id="nph:NP_1688A"/>
    <dbReference type="eggNOG" id="arCOG00505">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061385_1_0_2"/>
    <dbReference type="OrthoDB" id="197151at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07726">
        <property type="entry name" value="ST1585-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050855">
        <property type="entry name" value="NDM-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037482">
        <property type="entry name" value="ST1585_MBL-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42951:SF4">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE MBLAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42951">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="27"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="298" mass="32452" checksum="15807CF3E158C7C8" modified="2017-05-10" version="1">MGIGDVRSVESVVDCYYVDTGMYGTDGYGAVYIYDTERPALVDTGIGTHYDRILDALDAVGIGRSELEYIIPTHAHLDHAGGAGFLVEETAADVYTPEAAAQFLIDPSGLWEGTKRAVGEQIQFYTEPRPVPESRLEPLADGATLDLGDRELVLHDAPGHAFHQGILHEPDDAVVFAADAAGIYVPELDTVRETSPPADFDLEGVVEDAELIADLDPETICYGHFGPAPAADRIETYIAVITDWVESVEAARERLEDDEAVIEHFVEQSTIDEVWGETKGRAEVAMNVRGVLQYLDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVE0</accession>
    <name>A0A1U7EVE0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC-type transport system ATP-binding protein (Probable substrate glutamine/glutamate/polar amino acids)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">abc04a</name>
        <name evidence="10" type="ordered locus">NP_1778A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322613.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVE0"/>
    <dbReference type="STRING" id="348780.NP_1778A"/>
    <dbReference type="EnsemblBacteria" id="CAI48980">
        <property type="protein sequence ID" value="CAI48980"/>
        <property type="gene ID" value="NP_1778A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702373"/>
    <dbReference type="KEGG" id="nph:NP_1778A"/>
    <dbReference type="eggNOG" id="arCOG00923">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="OrthoDB" id="10909at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015424">
        <property type="term" value="F:ABC-type amino acid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03262">
        <property type="entry name" value="ABC_HisP_GlnQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030679">
        <property type="entry name" value="ABC_ATPase_HisP-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050086">
        <property type="entry name" value="MetN_ABC_transporter-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43166">
        <property type="entry name" value="AMINO ACID IMPORT ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43166:SF9">
        <property type="entry name" value="GLUTAMATE_ASPARTATE IMPORT ATP-BINDING PROTEIN GLTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039085">
        <property type="entry name" value="ABC_ATPase_HisP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="5"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI48980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="242" mass="26310" checksum="32780C8C6AD4FA21" modified="2017-05-10" version="1">MTELLSFDDVSKSYGDEQVLDGVSFAMEQSDVEVIIGPSGSGKSTMLRCVNRLTDIDDGAIYLDGHSIHDIGVNDLRRRVGMVFQDFNLFAHRTARGNITLGLRKVRGFSKEDAVAAADEHLERVGLADQAASYPAELSGGQKQRVGIARALAMDPELLLFDEPTSALDPELIGDVLSVMGDLADEGMTMLCVTHEMSFARSVADTITFLDDGRIAERGPPEQLFEAPETDRARDFLGEIDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVI5</accession>
    <name>A0A1U7EVI5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_1864A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322655.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVI5"/>
    <dbReference type="EnsemblBacteria" id="CAI49023">
        <property type="protein sequence ID" value="CAI49023"/>
        <property type="gene ID" value="NP_1864A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701281"/>
    <dbReference type="KEGG" id="nph:NP_1864A"/>
    <dbReference type="eggNOG" id="arCOG03920">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032880_0_0_2"/>
    <dbReference type="OrthoDB" id="169315at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045466">
        <property type="entry name" value="DUF6498"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20108">
        <property type="entry name" value="DUF6498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="281"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="305"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="428"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="456"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="228"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="228"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="479" mass="51315" checksum="8AACF92A26A1D699" modified="2017-05-10" version="1">MPSPGPPRGTTRPLTFAPMFLANLLPLVGVVWLDWSAETLVIIYVVEVLVVFPLAGLKALFAQQPPKEDRESGVISVTESDLVDKHGSVTIHERLPPVYPRNLPFASNVIAFTVWVAIFVGVAISGLGPLADAVHIPTVLVAVGGLLIGHVVELWREYFGQRRYEDVSPYAVVETPARQAFLLMFLLIVLPQTQPTAGTFVLGAFVFVKLLVDWSGFRAERGGGGRLTGWLSGPEPDAEASAAAPEPPSVPDADPSEVVATSRRSAALAAASAALTKRAPFHVPYLFVLWVLVSAFGLGGDASPLAALAVTGIIIGLFGVIVAGDVLEEILRHDAMEYRRCGDHLVAYDTRLETPQWAAPVDTLRGVDLVCDRFADRYYGTRTVTVTTGWGDSETERLVGPVDDPETLVAAFELPISDTDLTPFDRRVGAAVVAGAVLVVVAAAAMAFLPSVPPGGLFYLFFLFPVVALTLRGVWRLGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVQ0</accession>
    <name>A0A1U7EVQ0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CopG domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2068A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322754.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVQ0"/>
    <dbReference type="STRING" id="348780.NP_2068A"/>
    <dbReference type="EnsemblBacteria" id="CAI49125">
        <property type="protein sequence ID" value="CAI49125"/>
        <property type="gene ID" value="NP_2068A"/>
    </dbReference>
    <dbReference type="GeneID" id="43500087"/>
    <dbReference type="KEGG" id="nph:NP_2068A"/>
    <dbReference type="HOGENOM" id="CLU_3163228_0_0_2"/>
    <dbReference type="OrthoDB" id="382386at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="47" mass="5115" checksum="C81EB2B0DD1DF98E" modified="2017-05-10" version="1">MTSRDEAVDVEVAFEEGTLLQVDMLRQQPGYETRSDVVAAAIEAASD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVQ5</accession>
    <name>A0A1U7EVQ5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Fla cluster protein FlaG</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">flaG1</name>
        <name evidence="1" type="ordered locus">NP_2096A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322767.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVQ5"/>
    <dbReference type="STRING" id="348780.NP_2096A"/>
    <dbReference type="EnsemblBacteria" id="CAI49139">
        <property type="protein sequence ID" value="CAI49139"/>
        <property type="gene ID" value="NP_2096A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702712"/>
    <dbReference type="KEGG" id="nph:NP_2096A"/>
    <dbReference type="eggNOG" id="arCOG01822">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134827_0_0_2"/>
    <dbReference type="OrthoDB" id="183655at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097588">
        <property type="term" value="P:archaeal or bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002774">
        <property type="entry name" value="Flagellin_arc-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42200:SF2">
        <property type="entry name" value="ARCHAEAL FLAGELLA-RELATED PROTEIN F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42200">
        <property type="entry name" value="ARCHAEAL FLAGELLA-RELATED PROTEIN F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01917">
        <property type="entry name" value="Flagellin_arch-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="166" mass="17480" checksum="DD076C46807B2A3D" modified="2017-05-10" version="1">MSGISASTLIIFIASILVAAGVAGTLVTTVGDISTSAESQGDAISESINTDIEILNDGRGSSFYQEDVENNDGETVDALITLYARNAGSTSLHQDPDRINVLVNGQLITSENLNLESPNGGDDEVWAEDEVLEFEITLDEELDGSGNRVSISVDGSERAIEFPAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVR3</accession>
    <name>A0A1U7EVR3_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flagellin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">flg2</name>
        <name evidence="7" type="ordered locus">NP_2088A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Flagellin is the subunit protein which polymerizes to form the filaments of archaeal flagella.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Archaeal flagellum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the archaeal flagellin family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322763.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVR3"/>
    <dbReference type="STRING" id="348780.NP_2088A"/>
    <dbReference type="EnsemblBacteria" id="CAI49135">
        <property type="protein sequence ID" value="CAI49135"/>
        <property type="gene ID" value="NP_2088A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702719"/>
    <dbReference type="KEGG" id="nph:NP_2088A"/>
    <dbReference type="eggNOG" id="arCOG01829">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051124_1_0_2"/>
    <dbReference type="OrthoDB" id="102632at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097589">
        <property type="term" value="C:archaeal-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097588">
        <property type="term" value="P:archaeal or bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013373">
        <property type="entry name" value="Flagellin/pilin_N_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002774">
        <property type="entry name" value="Flagellin_arc-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02537">
        <property type="entry name" value="arch_flag_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35903">
        <property type="entry name" value="FLAGELLIN B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35903:SF1">
        <property type="entry name" value="FLAGELLIN B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01917">
        <property type="entry name" value="Flagellin_arch-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0974">Archaeal flagellum</keyword>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="7" id="KW-0282">Flagellum</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="107"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="183"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="207" mass="21856" checksum="B6B231FFD1D9E3E5" modified="2017-05-10" version="1">MFEQFTESEERGQVGIGTLIVFIALVLVAAIAAGVLINTAGFLQTQAQATGEESTEQVSTNLVFLSTTGTVSDAEDGIDSFNTTVQLGPGSSAIDLNDTTISVFTDDGQSQEFSGVDEDTDNNDGGIEFEDDSRDSSVLSTDSTDDDATATIEFDIGDANELSEPFEEGTTVDIVITTSEGTQAENSFIIEDPLDPQLEGGDEIRLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVR9</accession>
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        <submittedName>
            <fullName evidence="6">ArsR family transcription regulator</fullName>
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        <name evidence="6" type="ordered locus">NP_2114A</name>
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        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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            <taxon>Halobacteriales</taxon>
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            <taxon>Natronomonas</taxon>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
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        <property type="gene ID" value="NP_2114A"/>
    </dbReference>
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012318">
        <property type="entry name" value="HTH_CRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051011">
        <property type="entry name" value="Metal_resp_trans_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43132">
        <property type="entry name" value="ARSENICAL RESISTANCE OPERON REPRESSOR ARSR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43132:SF6">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REPRESSOR CZRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12840">
        <property type="entry name" value="HTH_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25212">
        <property type="entry name" value="HVO_A0114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00419">
        <property type="entry name" value="HTH_CRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="5">
        <location>
            <begin position="122"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI49148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="214" mass="21698" checksum="9992C7BEDBEF46C8" modified="2017-05-10" version="1">MDRQHVAVLVAVCMALALVPAGVAAAEEPAEAVAAATGPTVDATVTDRYRAGASAGLSRVDTDGVLEQPTRATIYEAIDESPGIDLAGLAAAADVTKSTARYHVDVLRDAGLVAGVEVAGALRFAPAELDAEVAGVLETDAPGTVLTAVADHEPASVRTLAEATDRAPSTVSHHLSELEARGLVERERRGEAVLTSLAPPARDALGAPPATADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVT6</accession>
    <name>A0A1U7EVT6_NATPD</name>
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        <submittedName>
            <fullName evidence="8">Flagellar motor/biogenesis protein FlaJ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">flaJ</name>
        <name evidence="8" type="ordered locus">NP_2160A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322799.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVT6"/>
    <dbReference type="STRING" id="348780.NP_2160A"/>
    <dbReference type="EnsemblBacteria" id="CAI49171">
        <property type="protein sequence ID" value="CAI49171"/>
        <property type="gene ID" value="NP_2160A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702716"/>
    <dbReference type="KEGG" id="nph:NP_2160A"/>
    <dbReference type="eggNOG" id="arCOG01809">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_479528_0_0_2"/>
    <dbReference type="OrthoDB" id="141855at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056569">
        <property type="entry name" value="ArlJ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018076">
        <property type="entry name" value="T2SS_GspF_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004704">
        <property type="entry name" value="PRK06041.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35402:SF2">
        <property type="entry name" value="FLAGELLA ACCESSORY PROTEIN J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35402">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00482">
        <property type="entry name" value="T2SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="8" id="KW-0282">Flagellum</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="209"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="237"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="321"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="463"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="515"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="547"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="domain" description="Type II secretion system protein GspF" evidence="7">
        <location>
            <begin position="97"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="576" mass="64846" checksum="FA4E16B901E6E769" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVV3</accession>
    <name>A0A1U7EVV3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">PQQ repeat protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2186A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322812.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVV3"/>
    <dbReference type="STRING" id="348780.NP_2186A"/>
    <dbReference type="EnsemblBacteria" id="CAI49184">
        <property type="protein sequence ID" value="CAI49184"/>
        <property type="gene ID" value="NP_2186A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701373"/>
    <dbReference type="KEGG" id="nph:NP_2186A"/>
    <dbReference type="eggNOG" id="arCOG02556">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027480_4_1_2"/>
    <dbReference type="OrthoDB" id="136681at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.630">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002372">
        <property type="entry name" value="PQQ_rpt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34512">
        <property type="entry name" value="CELL SURFACE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34512:SF30">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN ASSEMBLY FACTOR BAMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13360">
        <property type="entry name" value="PQQ_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyrrolo-quinoline quinone repeat" evidence="2">
        <location>
            <begin position="139"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrrolo-quinoline quinone repeat" evidence="2">
        <location>
            <begin position="245"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="389" mass="40671" checksum="480B243E61BD85D3" modified="2017-05-10" version="1">MDRRVTRRGVLAAAGIALAGCTDNGDDQPVRTPLPDEEREPLSNTWRSLGRDDGNTGAVAGIAGPYIEVAAEWQFDVGGAVIGGPVFDENTLYAGTADGEVYALDTHVGAERWSFSADGPVSTTPVVDDGLVAVGSSDGHIYGIDADDGTELWRFDTAVRVDSSPAIDDRYVYAGSVSNAAYAIDRTNGELHWRFDADGSVLASPAVGDETVYVASTDHNVYAIDPDSGEADWQFTTGGEISVAPTLVGDRLYVGSRDSSVYAIDTGSGEGRWSFGLEAAVASSPAVLGDRLFVGAADHNLYALGIGDDSPEGLWRSPTPGAAVGNPAVTDDHVFFGGRDGAVRALRTDGGDRSWEHDVDGGIQGGPALTDRWLYVGTDAGTVYAFRAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVV5</accession>
    <name>A0A1U7EVV5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PaaY family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2208A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322822.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVV5"/>
    <dbReference type="STRING" id="348780.NP_2208A"/>
    <dbReference type="EnsemblBacteria" id="CAI49195">
        <property type="protein sequence ID" value="CAI49195"/>
        <property type="gene ID" value="NP_2208A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701377"/>
    <dbReference type="KEGG" id="nph:NP_2208A"/>
    <dbReference type="eggNOG" id="arCOG01849">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064827_4_2_2"/>
    <dbReference type="OrthoDB" id="10940at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd04645">
        <property type="entry name" value="LbH_gamma_CA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047324">
        <property type="entry name" value="LbH_gamma_CA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050484">
        <property type="entry name" value="Transf_Hexapept/Carb_Anhydrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13061">
        <property type="entry name" value="DYNACTIN SUBUNIT P25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13061:SF29">
        <property type="entry name" value="GAMMA CARBONIC ANHYDRASE-LIKE 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="177" mass="18850" checksum="5CB49905A4005866" modified="2017-05-10" version="1">MLRSFDGMEPTVHEDAYVDPAAVVIGDVTIEKDASVWPNVTLRGDHGEIILREGANVQDNAVLHEGTEIGPYATVGHTAIVHSAAVERRALVGMSATVLDGSVVGERAMVGANSLVTEGTDIEPETLYAGTPAEKLKDVEESPWAYAGDRYVELSREHIESSEVLDDDYSGSAPTDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVZ4</accession>
    <name>A0A1U7EVZ4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="13">NADH dehydrogenase-like complex subunit B</fullName>
            <ecNumber evidence="13">1.6.5.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">nuoB</name>
        <name evidence="13" type="ordered locus">NP_2294A</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the complex I 20 kDa subunit family.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.-" evidence="13"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322865.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVZ4"/>
    <dbReference type="STRING" id="348780.NP_2294A"/>
    <dbReference type="EnsemblBacteria" id="CAI49238">
        <property type="protein sequence ID" value="CAI49238"/>
        <property type="gene ID" value="NP_2294A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702990"/>
    <dbReference type="KEGG" id="nph:NP_2294A"/>
    <dbReference type="eggNOG" id="arCOG01554">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055737_6_0_2"/>
    <dbReference type="OrthoDB" id="5740at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12280:FF:000006">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 20 kDa subunit,mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01356">
        <property type="entry name" value="NDH1_NuoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006138">
        <property type="entry name" value="NADH_UQ_OxRdtase_20Kd_su"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01957">
        <property type="entry name" value="nuoB_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005012">
        <property type="entry name" value="PRK06411.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11995">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11995:SF14">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56770">
        <property type="entry name" value="HydA/Nqo6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8 10" id="KW-0408">Iron</keyword>
    <keyword evidence="9 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0520">NAD</keyword>
    <keyword evidence="7 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase-like 20kDa subunit" evidence="12">
        <location>
            <begin position="64"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAI49238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="227" mass="25518" checksum="7F08725BCB7E65D5" modified="2017-05-10" version="1">MSSEKLHDTTSTQQARMGDGVDDRFNSKLREAFGSTPFILTKFDKFMNWVRGNSMFMLQFGIACCSIEMIHTYAIKHDLDRFGAGVPRASPRQADVIIVPGTIVSKFAPRMKRVYDQMPEPKFVVGMGSCTISGGPFQEGYNVIKGAEEVIPVDIHVPGCPPRPEALIYGIAKLQERIRNGETSPVTVKPYELEEFGDLERDELVQHLADQIDEEDLVMRYNWADSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVZ5</accession>
    <name>A0A1U7EVZ5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Transcription initiation factor IIB</fullName>
            <shortName evidence="10">TFIIB</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">tfbA3</name>
        <name evidence="10" type="synonym">tfb</name>
        <name evidence="14" type="ordered locus">NP_2246A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre-initiation complex (DNA-TBP-TFIIB).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the TFIIB family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322841.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVZ5"/>
    <dbReference type="STRING" id="348780.NP_2246A"/>
    <dbReference type="EnsemblBacteria" id="CAI49214">
        <property type="protein sequence ID" value="CAI49214"/>
        <property type="gene ID" value="NP_2246A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703235"/>
    <dbReference type="KEGG" id="nph:NP_2246A"/>
    <dbReference type="eggNOG" id="arCOG01981">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043736_0_0_2"/>
    <dbReference type="OrthoDB" id="7429at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20549">
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            <begin position="20"/>
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    <feature type="binding site" evidence="10">
        <location>
            <position position="24"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
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    <feature type="binding site" evidence="10">
        <location>
            <position position="27"/>
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        <ligand>
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        <source>
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    </evidence>
    <sequence length="319" mass="35544" checksum="7D1EF83D416CB6C3" modified="2017-05-10" version="1">MSEHIRTRTTETETEDERAETSGCPECGGKLAADTEHGETVCRDCGLVVEEDEIDRGPEWRAFDSAEKDEKSRVGAPTTNMMHDKGLSTNIDWRDQDAYGNSLDSRQRQKMQRLRKWNERFRTRDSKERNLKQALGEIDRMASALGLPDNVRETASVIYRRALDENLLPGRSIEGVSTASVYAAARQAGVPRSLDEVAGVSRVEKSEIARTYRYVVRELGLEVQPADPESYVPRFASELELSEEAENRARQLLQNAKEQGVHSGKSPVGLAAAAVYAASLLTNEKTTQAAVSEVADISEVTIRNRYHELLEAEQDLAFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVZ8</accession>
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        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
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            <dbReference type="PubMed" id="16169924"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
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    </dbReference>
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            <end position="52"/>
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            <begin position="59"/>
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        <source>
            <dbReference type="EMBL" id="CAI49243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="91" mass="9688" checksum="E6A1B1804DB2B50A" modified="2017-05-10" version="1">MAVVPLETLAFLLFALLTVGASLGVVLARDVWHSALFLGAALFSVAVHYVMLRAEFLAVIQVLVYVGGVLILITFAVMLVRQDNTGGEALT</sequence>
</entry>
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    <accession>A0A1U7EW08</accession>
    <name>A0A1U7EW08_NATPD</name>
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        <recommendedName>
            <fullName evidence="2">DUF7847 domain-containing protein</fullName>
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        <name evidence="3" type="ordered locus">NP_2326A</name>
    </gene>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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                <person name="Pfeiffer F."/>
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            </authorList>
            <dbReference type="PubMed" id="16169924"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAI49254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A1U7EW08"/>
    <dbReference type="STRING" id="348780.NP_2326A"/>
    <dbReference type="EnsemblBacteria" id="CAI49254">
        <property type="protein sequence ID" value="CAI49254"/>
        <property type="gene ID" value="NP_2326A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701406"/>
    <dbReference type="KEGG" id="nph:NP_2326A"/>
    <dbReference type="eggNOG" id="arCOG03934">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084120_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057169">
        <property type="entry name" value="DUF7847"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25231">
        <property type="entry name" value="DUF7847"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
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    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7847" evidence="2">
        <location>
            <begin position="3"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="248" mass="25315" checksum="E6D31EFB7DCA2649" modified="2017-05-10" version="1">MALPIGQTIADGIERAATKTGLVLAVVLIAYQLPLVAATNTLFEALLPAEAAAEADIGLTLPLSADAAAAIAVLGYLFGVAVAVVAARALARPHAALSTFPPSLYTRRILRATLAALVAGLLATVAIGVGTLLLVIPGLFLLVSFAFYLVVIGVEDEGPLSSLRRSWQLASGNRWRLFALILIVGIAVGLVSSVVSIAAAVTGSMVASEVASILVAGFLGIVGYGVIIEAYLWLRENDDPAAAATASM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW28</accession>
    <name>A0A1U7EW28_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">PadR family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2366A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322900.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW28"/>
    <dbReference type="STRING" id="348780.NP_2366A"/>
    <dbReference type="EnsemblBacteria" id="CAI49274">
        <property type="protein sequence ID" value="CAI49274"/>
        <property type="gene ID" value="NP_2366A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701424"/>
    <dbReference type="KEGG" id="nph:NP_2366A"/>
    <dbReference type="eggNOG" id="arCOG00006">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151709_0_0_2"/>
    <dbReference type="OrthoDB" id="262522at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator PadR N-terminal" evidence="2">
        <location>
            <begin position="33"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="118" mass="12756" checksum="22307AA550D1C7B4" modified="2017-05-10" version="1">MGSETTAGEARTDTDALPNKKPADLSKFQLRILAVLADAGDAPKGVTVKRRLQEYYSSDVNPGQLYPNLNELVETGFVAKGQKDKRTNAYEITNDGQQALTSEVKWLAARTAGVAEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW47</accession>
    <name>A0A1U7EW47_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nicotinate-nucleotide pyrophosphorylase [carboxylating]</fullName>
            <ecNumber evidence="6">2.4.2.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Quinolinate phosphoribosyltransferase [decarboxylating]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">nadC</name>
        <name evidence="9" type="ordered locus">NP_2414A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the catabolism of quinolinic acid (QA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>nicotinate beta-D-ribonucleotide + CO2 + diphosphate = quinolinate + 5-phospho-alpha-D-ribose 1-diphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:12733"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29959"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from quinolinate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Hexamer formed by 3 homodimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the NadC/ModD family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.19" evidence="6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322924.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW47"/>
    <dbReference type="STRING" id="348780.NP_2414A"/>
    <dbReference type="EnsemblBacteria" id="CAI49298">
        <property type="protein sequence ID" value="CAI49298"/>
        <property type="gene ID" value="NP_2414A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702963"/>
    <dbReference type="KEGG" id="nph:NP_2414A"/>
    <dbReference type="eggNOG" id="arCOG01482">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039622_2_0_2"/>
    <dbReference type="OrthoDB" id="115072at2157"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00331"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004514">
        <property type="term" value="F:nicotinate-nucleotide diphosphorylase (carboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034213">
        <property type="term" value="P:quinolinate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01572">
        <property type="entry name" value="QPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000030">
        <property type="entry name" value="Nicotinate-nucleotide pyrophosphorylase, carboxylating"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.20">
        <property type="entry name" value="Quinolinate phosphoribosyl transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004393">
        <property type="entry name" value="NadC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027277">
        <property type="entry name" value="NadC/ModD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037128">
        <property type="entry name" value="Quinolinate_PRibosylTase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002638">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022412">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00078">
        <property type="entry name" value="nadC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32179">
        <property type="entry name" value="NICOTINATE-NUCLEOTIDE PYROPHOSPHORYLASE [CARBOXYLATING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32179:SF3">
        <property type="entry name" value="NICOTINATE-NUCLEOTIDE PYROPHOSPHORYLASE [CARBOXYLATING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01729">
        <property type="entry name" value="QRPTase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02749">
        <property type="entry name" value="QRPTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006250">
        <property type="entry name" value="NadC_ModD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54675">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3 6" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Quinolinate phosphoribosyl transferase N-terminal" evidence="8">
        <location>
            <begin position="28"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Quinolinate phosphoribosyl transferase C-terminal" evidence="7">
        <location>
            <begin position="101"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR006250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI49298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="272" mass="28140" checksum="B8F4C018BDB98241" modified="2017-05-10" version="1">MRIEQSQVERWLREDLGHHDVTNEVPGQTTGRLVATEPGVLAGLEAAAAVFEYLGVDIESRRSAGDAVEAGETVLRVSGAASDVLRGERVAVNIAGHASGVATKTRTAVERARAVAPDVAIAGTRKTTPGLRGVEKRAIAAGGGDTHRLDLSHMVMVKDNHIAEMGVETAIERFREQASFATQIEAEAETAEMAVAAAEAGADIVLLDNMSPSETADAAAAVPDGTLTEASGGITLNDVVDYAGTGVDIISLGSLTHSAGALDFSFRTGGDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW74</accession>
    <name>A0A1U7EW74_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ArsR family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">NP_2490A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322962.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW74"/>
    <dbReference type="STRING" id="348780.NP_2490A"/>
    <dbReference type="EnsemblBacteria" id="CAI49336">
        <property type="protein sequence ID" value="CAI49336"/>
        <property type="gene ID" value="NP_2490A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701463"/>
    <dbReference type="KEGG" id="nph:NP_2490A"/>
    <dbReference type="eggNOG" id="arCOG01684">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090889_0_0_2"/>
    <dbReference type="OrthoDB" id="9623at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000591">
        <property type="entry name" value="Archaeal heat shock regulator, ArsR family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056346">
        <property type="entry name" value="HTH_Cmi2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051081">
        <property type="entry name" value="HTH_MetalResp_TranReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033788">
        <property type="entry name" value="HTH_metalloreg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33154">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ARSR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33154:SF33">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR SDPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24270">
        <property type="entry name" value="HTH_Cmi2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="115"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="133"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI49336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="265" mass="29022" checksum="4446254308055DF0" modified="2017-05-10" version="1">MDSAALLDLLGNENRRRILRLLARRPCYVTEISDYLGVSPKAVIDHLRKLEEAGLVESHVDDGRRKYFSIARNLRLEVNVTPYDFGTKSAYSASPNLDITSWKHVSLHVERATQAAAELNSEEETEDTDIRTDAAGASDSPPADVADGASDSDSPGDADQPPNADLAGLAADLEQLERLENELSMAQRWVQGRLTETLDRISDHFDGVDGRFYAELLRGLATGPEPPDRLAQRVDAPPDVVADALEGLAAHGVVERDGDQWRLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWG6</accession>
    <name>A0A1U7EWG6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2678A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323055.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWG6"/>
    <dbReference type="STRING" id="348780.NP_2678A"/>
    <dbReference type="EnsemblBacteria" id="CAI49430">
        <property type="protein sequence ID" value="CAI49430"/>
        <property type="gene ID" value="NP_2678A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701522"/>
    <dbReference type="KEGG" id="nph:NP_2678A"/>
    <dbReference type="eggNOG" id="arCOG04637">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_181982_0_0_2"/>
    <dbReference type="OrthoDB" id="311268at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055967">
        <property type="entry name" value="DUF7545"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24411">
        <property type="entry name" value="DUF7545"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="94" mass="10494" checksum="47DFEC4725C1FCA9" modified="2017-05-10" version="1">MSDTTVTLHIEGPNSEDEVTLPAEMLELMRESDESDAEIVGDLALFSCAQRIHATVHHAEGGDTEEYRAIEEETMELFEERFGASYGELTGHQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWJ9</accession>
    <name>A0A1U7EWJ9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2748A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323090.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWJ9"/>
    <dbReference type="EnsemblBacteria" id="CAI49465">
        <property type="protein sequence ID" value="CAI49465"/>
        <property type="gene ID" value="NP_2748A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701544"/>
    <dbReference type="KEGG" id="nph:NP_2748A"/>
    <dbReference type="eggNOG" id="arCOG11367">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2032929_0_0_2"/>
    <dbReference type="OrthoDB" id="275687at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055898">
        <property type="entry name" value="DUF7475"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24287">
        <property type="entry name" value="DUF7475"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="121" mass="13227" checksum="721FBBCA091C675E" modified="2017-05-10" version="1">MTRRTLADLRLPNLLFICLTLSLAGIHLYLGLTAPFIEPGRAGQFALIGVAFLAGVVAYFTPYWRPVLYLLGVVFAVFLAGVWAFAGFEHARVGIVTGVVATAFVMLALYLFVREEFRAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX10</accession>
    <name>A0A1U7EX10_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Homolog to rhodopsin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3132A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323279.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX10"/>
    <dbReference type="STRING" id="348780.NP_3132A"/>
    <dbReference type="EnsemblBacteria" id="CAI49657">
        <property type="protein sequence ID" value="CAI49657"/>
        <property type="gene ID" value="NP_3132A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701663"/>
    <dbReference type="KEGG" id="nph:NP_3132A"/>
    <dbReference type="eggNOG" id="arCOG02810">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1182857_0_0_2"/>
    <dbReference type="OrthoDB" id="330248at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="237" mass="25142" checksum="E47C070CAFC995FB" modified="2017-05-10" version="1">MTEALATDATVYRLFAAAYAVAAVVVLAATTRLPDRHRKYGYAFVGLFSLTAITSVVWSAGFGAVNGAGIPRLLEDYVAYLGIFAAIAYIGGASRRIIVLTAASMFAIRTFFELGSVLDGALGLAMLSLVVVGFVVLTSLLLWLGRQSRGTISESRYLLFWKTRNLLLFLFGMLIIVAVLPILGVLDGFSGNILVEYVDLLIRAGFAGFVLNNLDVLDERSAGGAVDLGDTSAAAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX53</accession>
    <name>A0A1U7EX53_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DHH/RecJ family phosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3224A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323325.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX53"/>
    <dbReference type="STRING" id="348780.NP_3224A"/>
    <dbReference type="EnsemblBacteria" id="CAI49703">
        <property type="protein sequence ID" value="CAI49703"/>
        <property type="gene ID" value="NP_3224A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701690"/>
    <dbReference type="KEGG" id="nph:NP_3224A"/>
    <dbReference type="eggNOG" id="arCOG00429">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018957_0_0_2"/>
    <dbReference type="OrthoDB" id="5596at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04487">
        <property type="entry name" value="RecJ_OBF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04473">
        <property type="entry name" value="S1_RecJ_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1640.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="DHH phosphoesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S1 motif" evidence="1">
        <location>
            <begin position="33"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="635" mass="69836" checksum="7C457F0A0F57492E" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX58</accession>
    <name>A0A1U7EX58_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Homolog to phage PhiH1 repressor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3254A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49718.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939584.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX58"/>
    <dbReference type="EnsemblBacteria" id="CAI49718">
        <property type="protein sequence ID" value="CAI49718"/>
        <property type="gene ID" value="NP_3254A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701703"/>
    <dbReference type="KEGG" id="nph:NP_3254A"/>
    <dbReference type="eggNOG" id="arCOG03924">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161782_3_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49718.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="102" mass="11608" checksum="915ACC5B942028C4" modified="2017-05-10" version="1">MRPRVPWMNETDDAILEFYLKLDEADGLRVALPPTAVWYNLVKKEALLDKGSSTISRRMSRLDKIGLLNLVDSDRAYYQFTDKGAAYLAGDLEADELELPDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX83</accession>
    <name>A0A1U7EX83_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF7115 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_3354A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323388.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX83"/>
    <dbReference type="EnsemblBacteria" id="CAI49768">
        <property type="protein sequence ID" value="CAI49768"/>
        <property type="gene ID" value="NP_3354A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701746"/>
    <dbReference type="KEGG" id="nph:NP_3354A"/>
    <dbReference type="eggNOG" id="arCOG04660">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058951_0_0_2"/>
    <dbReference type="OrthoDB" id="307384at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055539">
        <property type="entry name" value="DUF7115"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23428">
        <property type="entry name" value="DUF7115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7115" evidence="3">
        <location>
            <begin position="3"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="206"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="300"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="339" mass="36192" checksum="6F49577363CD67A3" modified="2017-05-10" version="1">MDDLPELLADAVAGGTVIDIIDIGGGDTIAVTRSATHVFRSDGLLKDESVETFGHDVDRIGVKTKRRKSSIQLESIDASESFTVPSKIADAVVEATLEGVLRTTGVVAADEELQTQFRFSELTLVVTDRQLLKHVGSAVWDDEYEAFRYDELTGLDFEKGSVATQVIVETDSRRQRVKVPNEHAGSVQQVVQTAVFEYHGVSSLSGLQAKLDPETEPEPVGGDEAADADEAEATTSPEENGGQDDDDGFVSADWSPPADQDPARVTPNSDVDGNDAAAGAAESQPTADATTATPDAAADVEALSQQVEALAAKVDRQTELLEAQQETIEQLVDELRRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX89</accession>
    <name>A0A1U7EX89_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Vitamin B12-dependent ribonucleotide reductase</fullName>
            <ecNumber evidence="3 13">1.17.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">nrdJ</name>
        <name evidence="18" type="ordered locus">NP_3346A</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 13">Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + [thioredoxin]-disulfide + H2O = a ribonucleoside 5'-diphosphate + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:23252"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="EC" id="1.17.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>adenosylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the ribonucleoside diphosphate reductase class-2 family.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="3 13"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49764.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939838.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX89"/>
    <dbReference type="STRING" id="348780.NP_3346A"/>
    <dbReference type="EnsemblBacteria" id="CAI49764">
        <property type="protein sequence ID" value="CAI49764"/>
        <property type="gene ID" value="NP_3346A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702984"/>
    <dbReference type="KEGG" id="nph:NP_3346A"/>
    <dbReference type="eggNOG" id="arCOG04276">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000404_2_1_2"/>
    <dbReference type="OrthoDB" id="6188at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009263">
        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02888">
        <property type="entry name" value="RNR_II_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050862">
        <property type="entry name" value="RdRp_reductase_class-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000788">
        <property type="entry name" value="RNR_lg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013509">
        <property type="entry name" value="RNR_lsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013344">
        <property type="entry name" value="RNR_NrdJ/NrdZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024434">
        <property type="entry name" value="TSCPD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02504">
        <property type="entry name" value="NrdJ_Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43371:SF1">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43371">
        <property type="entry name" value="VITAMIN B12-DEPENDENT RIBONUCLEOTIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02867">
        <property type="entry name" value="Ribonuc_red_lgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00317">
        <property type="entry name" value="Ribonuc_red_lgN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12637">
        <property type="entry name" value="TSCPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01183">
        <property type="entry name" value="RIBORDTASEM1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51998">
        <property type="entry name" value="PFL-like glycyl radical enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0846">Cobalamin</keyword>
    <keyword evidence="10 13" id="KW-0170">Cobalt</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 13" id="KW-0237">DNA synthesis</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 13 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribonucleotide reductase large subunit N-terminal" evidence="15">
        <location>
            <begin position="30"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Ribonucleotide reductase large subunit C-terminal" evidence="16">
        <location>
            <begin position="175"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="domain" description="TSCPD" evidence="17">
        <location>
            <begin position="847"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="967"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="971"/>
            <end position="980"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU364064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF12637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAI49764.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="1041" mass="114674" checksum="33C7D53A3AF29FA1" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXF2</accession>
    <name>A0A1U7EXF2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Glycoside hydrolase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3522A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
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    </dbReference>
    <dbReference type="GO" id="GO:0016787">
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        <property type="project" value="UniProtKB-KW"/>
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        <source>
            <dbReference type="EMBL" id="CAI49852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="288" mass="32823" checksum="20EFE005F9ACF365" modified="2017-05-10" version="1">MYGVVTRNEEETRWDDFDRAFYEVKDVSGRSSEPAAETVSMVSCFGDNAAAEENPELVPVTPDGERATREQTYFDWAYICPTHDDYRAGLLEIIADCADVTPDVRLDDVGFPRPEYCYCDRCNEHFESWVRNRHEAGEGPPPEETGVEDRYEWRSEVITSFVAEAADRIPGRTYLTLYPDPYEGHLYERAGLDIGALESYVDEFIVPLYDTSYGTTYWLEAIAKGFESRLDTPFSIELYAVDVDIDNLIHATEVAEQYGESVLFGYDAANGRAALRRRRADAREGVEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
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        </submittedName>
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        <name evidence="2" type="ordered locus">NP_3550A</name>
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        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <lineage>
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            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXI1"/>
    <dbReference type="STRING" id="348780.NP_3550A"/>
    <dbReference type="EnsemblBacteria" id="CAI49866">
        <property type="protein sequence ID" value="CAI49866"/>
        <property type="gene ID" value="NP_3550A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701803"/>
    <dbReference type="KEGG" id="nph:NP_3550A"/>
    <dbReference type="eggNOG" id="arCOG13469">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2079488_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="117" mass="11504" checksum="3F7A546CB855ED54" modified="2017-05-10" version="1">MSEPDGARRLRGGGVGSVLGGLAVGAFALVASYPTAAGAVLGIGIAGFVAERGRSLDSRISLGFVAVGGIGLLEATGTTAVGIDPFLLASFGVTFGLIDIGLSSVLGRAKNRSNGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXJ0</accession>
    <name>A0A1U7EXJ0_NATPD</name>
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        <submittedName>
            <fullName evidence="11">Kef-type transport system</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">kef5</name>
        <name evidence="11" type="synonym">tp41b</name>
        <name evidence="11" type="ordered locus">NP_3604A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family.</text>
    </comment>
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        <property type="protein sequence ID" value="CAI49893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A1U7EXJ0"/>
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    <dbReference type="EnsemblBacteria" id="CAI49893">
        <property type="protein sequence ID" value="CAI49893"/>
        <property type="gene ID" value="NP_3604A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703319"/>
    <dbReference type="KEGG" id="nph:NP_3604A"/>
    <dbReference type="eggNOG" id="arCOG01955">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="43518at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42751">
        <property type="entry name" value="SODIUM/HYDROGEN EXCHANGER FAMILY/TRKA DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42751:SF3">
        <property type="entry name" value="SODIUM_GLUTAMATE SYMPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
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            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
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            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="320"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="349"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="9">
        <location>
            <begin position="15"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="10">
        <location>
            <begin position="406"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="533"/>
            <end position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI49893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="576" mass="61475" checksum="C0B36099DA64984E" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXP5</accession>
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        <submittedName>
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        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">NP_3776A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
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            <taxon>Stenosarchaea group</taxon>
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            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323596.1">
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    <dbReference type="AlphaFoldDB" id="A0A1U7EXP5"/>
    <dbReference type="STRING" id="348780.NP_3776A"/>
    <dbReference type="EnsemblBacteria" id="CAI49979">
        <property type="protein sequence ID" value="CAI49979"/>
        <property type="gene ID" value="NP_3776A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701862"/>
    <dbReference type="KEGG" id="nph:NP_3776A"/>
    <dbReference type="eggNOG" id="arCOG00008">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130789_0_0_2"/>
    <dbReference type="OrthoDB" id="56053at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator PadR N-terminal" evidence="1">
        <location>
            <begin position="76"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="166" mass="18031" checksum="01607A1AA26798E7" modified="2017-05-10" version="1">MTEMDPTAQLGSAESDQSITVSQLRAELDGTGQSEAPGTAISEVVDEVEASLFGTDGFSFEETHVKSSLDELLLSLVALRSSDTHGKQLLDDLAQEFDTILSPGTVYPRLHDLCDEDVLEQRELVKTKEYTLDDATEAQSDIAEAARQHLALGMVLRAALEEGDFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXQ3</accession>
    <name>A0A1U7EXQ3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Homolog to phage PhiH1 repressor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3820A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323618.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXQ3"/>
    <dbReference type="STRING" id="348780.NP_3820A"/>
    <dbReference type="EnsemblBacteria" id="CAI50001">
        <property type="protein sequence ID" value="CAI50001"/>
        <property type="gene ID" value="NP_3820A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701873"/>
    <dbReference type="KEGG" id="nph:NP_3820A"/>
    <dbReference type="eggNOG" id="arCOG03924">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2420137_0_0_2"/>
    <dbReference type="OrthoDB" id="285635at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005471">
        <property type="entry name" value="Tscrpt_reg_IclR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09339">
        <property type="entry name" value="HTH_IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH iclR-type" evidence="1">
        <location>
            <begin position="20"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="91" mass="10349" checksum="89CA52E1433DF171" modified="2017-05-10" version="1">MATNHSPVRIDDQLHPADERILEYFAEEPPDYVPLAANRLGMHLGYVERRVETLVENGLLEPVSQERIYTITDRGQRCVESETALRADGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXQ8</accession>
    <name>A0A1U7EXQ8_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7969 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3756A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXQ8"/>
    <dbReference type="STRING" id="348780.NP_3756A"/>
    <dbReference type="EnsemblBacteria" id="CAI49969">
        <property type="protein sequence ID" value="CAI49969"/>
        <property type="gene ID" value="NP_3756A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3756A"/>
    <dbReference type="eggNOG" id="arCOG06411">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140135_0_0_2"/>
    <dbReference type="OrthoDB" id="313263at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058275">
        <property type="entry name" value="DUF7969"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25923">
        <property type="entry name" value="DUF7969"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7969" evidence="1">
        <location>
            <begin position="3"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="94" mass="10208" checksum="3F9CDA21D89DCB59" modified="2017-05-10" version="1">MPAVSYYCPRCETTVELQRDGYLADKSVTPYPLDGWDYVTADEDYDTPEADGVRFVCGEDDATRDPGAAGCGEPFYLSFVKHGTTPAGAALDSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXR8</accession>
    <name>A0A1U7EXR8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BolA family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">bolA</name>
        <name evidence="2" type="ordered locus">NP_3780A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BolA/IbaG family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323598.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXR8"/>
    <dbReference type="STRING" id="348780.NP_3780A"/>
    <dbReference type="EnsemblBacteria" id="CAI49981">
        <property type="protein sequence ID" value="CAI49981"/>
        <property type="gene ID" value="NP_3780A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702556"/>
    <dbReference type="KEGG" id="nph:NP_3780A"/>
    <dbReference type="eggNOG" id="arCOG04647">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109462_4_2_2"/>
    <dbReference type="OrthoDB" id="155092at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.90">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002634">
        <property type="entry name" value="BolA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036065">
        <property type="entry name" value="BolA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050961">
        <property type="entry name" value="BolA/IbaG_stress_morph_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46229">
        <property type="entry name" value="BOLA TRANSCRIPTION REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46229:SF2">
        <property type="entry name" value="BOLA-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01722">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003113">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82657">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="77" mass="8478" checksum="D28CA3A72C097730" modified="2017-05-10" version="1">MTPEDVEAEIEAALEDCEATVSRARGKHDDDHLEATVVSPAFEELSLVDQHQLVYDALGEAMTTDIHALELTTRTPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXS0</accession>
    <name>A0A1U7EXS0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Probable tRNA sulfurtransferase</fullName>
            <ecNumber evidence="8">2.8.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Sulfur carrier protein ThiS sulfurtransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Thiamine biosynthesis protein ThiI</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">tRNA 4-thiouridine synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 11" type="primary">thiI</name>
        <name evidence="11" type="ordered locus">NP_3782A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>[ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + a uridine in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + ATP + H(+) = [ThiI sulfur-carrier protein]-L-cysteine + a 4-thiouridine in tRNA + 2 oxidized [2Fe-2S]-[ferredoxin] + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:24176"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:13337"/>
            <dbReference type="Rhea" id="RHEA-COMP:13338"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:13340"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:136798"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.8.1.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>[ThiS sulfur-carrier protein]-C-terminal Gly-Gly-AMP + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] + AH2 = [ThiS sulfur-carrier protein]-C-terminal-Gly-aminoethanethioate + L-cysteinyl-[cysteine desulfurase] + A + AMP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:43340"/>
            <dbReference type="Rhea" id="RHEA-COMP:12157"/>
            <dbReference type="Rhea" id="RHEA-COMP:12158"/>
            <dbReference type="Rhea" id="RHEA-COMP:12910"/>
            <dbReference type="Rhea" id="RHEA-COMP:19908"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:90618"/>
            <dbReference type="ChEBI" id="CHEBI:232372"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the ThiI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.4" evidence="8"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323599.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXS0"/>
    <dbReference type="STRING" id="348780.NP_3782A"/>
    <dbReference type="EnsemblBacteria" id="CAI49982">
        <property type="protein sequence ID" value="CAI49982"/>
        <property type="gene ID" value="NP_3782A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703249"/>
    <dbReference type="KEGG" id="nph:NP_3782A"/>
    <dbReference type="eggNOG" id="arCOG00038">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037952_4_0_2"/>
    <dbReference type="OrthoDB" id="372227at2157"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004810">
        <property type="term" value="F:CCA tRNA nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140741">
        <property type="term" value="F:tRNA-uracil-4 sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052837">
        <property type="term" value="P:thiazole biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002937">
        <property type="term" value="P:tRNA 4-thiouridine biosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11716">
        <property type="entry name" value="THUMP_ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2130.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00021">
        <property type="entry name" value="ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020536">
        <property type="entry name" value="ThiI_AANH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054173">
        <property type="entry name" value="ThiI_fer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049962">
        <property type="entry name" value="THUMP_ThiI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003720">
        <property type="entry name" value="tRNA_STrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050102">
        <property type="entry name" value="tRNA_sulfurtransferase_ThiI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43209">
        <property type="entry name" value="TRNA SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43209:SF1">
        <property type="entry name" value="TRNA SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02568">
        <property type="entry name" value="ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22025">
        <property type="entry name" value="ThiI_fer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143437">
        <property type="entry name" value="THUMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 8" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 8" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="THUMP" evidence="10">
        <location>
            <begin position="58"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="178"/>
            <end position="179"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI49982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="383" mass="40580" checksum="7B3DAE9A1E0FDCF1" modified="2017-05-10" version="1">MDCTVVRYGELGVKGQSVRRRMETRLRENIAAALESDGISGSVERRHGRLVVHTTAPEAAARAVTAVPGVASASPAVRVSPEAAEIEAALADLARGREFDSYAVRASRARDDHPFTSPELEQSGGAAVGEVTDATVDLEAPAVTFEADVRPDVAYVFAARHEGPGGLPVGTQAPLVALVSGGIDSPVAAAEMLRRGAPVIPVYVDLGDYGGVDHRARAISTCERLAARAPNTDCRPYVVDGGDTLDDIASALDRGRMLSVRRYFFAVAEAIAEIEDAAGIVTGEALGQKSSQTARNLQVTSAAVELPVHRPLLSMDKPEITERARKLGTYEEATIQAGCNRLAPDTPETDGRLEKLRDLEPDNLLERAREDALAATRVSLSDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXV0</accession>
    <name>A0A1U7EXV0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DUF790 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3848A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323632.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXV0"/>
    <dbReference type="STRING" id="348780.NP_3848A"/>
    <dbReference type="EnsemblBacteria" id="CAI50015">
        <property type="protein sequence ID" value="CAI50015"/>
        <property type="gene ID" value="NP_3848A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701885"/>
    <dbReference type="KEGG" id="nph:NP_3848A"/>
    <dbReference type="eggNOG" id="arCOG04356">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038336_0_0_2"/>
    <dbReference type="OrthoDB" id="57367at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008508">
        <property type="entry name" value="Bax1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39640:SF1">
        <property type="entry name" value="DUF790 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39640">
        <property type="entry name" value="VNG6129C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05626">
        <property type="entry name" value="DUF790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019435">
        <property type="entry name" value="UCP019435"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="450" mass="51429" checksum="F67C24B4E78D3254" modified="2017-05-10" version="1">MLTADLARSRTYEDTITPLFIDTDDQRYRDTAEELIHIFEEHLGGPKGELEDTIDQLTAADTDYKIVQGLAKLLKDECEFETVAPVAPQRIREELFAVANDNYPIVRQPTLGEDTKSIWVYSTVAERLGITLEECYRGMYADLEENQQLVRFGHRVSDEYEGVEDSTTTTRLTGNSEESYAEDTVTVDWLLTRYNLALAQAVLYDATEMRVRVWDSFATVFSYVKLFGLMHRIYPIDRDGNRVPSTDVADGYEAVLDGAVSLFSKSRKYGIRMANFLPALPLCDRWEMEADILDDDSGPSSRTLSFELDHTEGLSSHYSAQSDFDSDVERTLAQKWERANTDWELIREDDVLDLGAEVMLPDFAVEHPDGRRVLFEIVGFWTPEYLDEKLAKIRDADRDNLIVAVSERLDCSADDFEGMDDRVLWFKSGIHVYDVVELAEEYAVETTLEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY32</accession>
    <name>A0A1U7EY32_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Pyruvate--ferredoxin oxidoreductase beta subunit</fullName>
            <ecNumber evidence="5">1.2.7.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">porB</name>
        <name evidence="5" type="ordered locus">NP_4044A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="1.2.7.1" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323729.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY32"/>
    <dbReference type="STRING" id="348780.NP_4044A"/>
    <dbReference type="EnsemblBacteria" id="CAI50113">
        <property type="protein sequence ID" value="CAI50113"/>
        <property type="gene ID" value="NP_4044A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703044"/>
    <dbReference type="KEGG" id="nph:NP_4044A"/>
    <dbReference type="eggNOG" id="arCOG01599">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048564_0_0_2"/>
    <dbReference type="OrthoDB" id="296931at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03375">
        <property type="entry name" value="TPP_OGFOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051457">
        <property type="entry name" value="2-oxoacid:Fd_oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011896">
        <property type="entry name" value="OFOB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02177">
        <property type="entry name" value="PorB_KorB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48084:SF4">
        <property type="entry name" value="2-OXOGLUTARATE OXIDOREDUCTASE SUBUNIT KORB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48084">
        <property type="entry name" value="2-OXOGLUTARATE OXIDOREDUCTASE SUBUNIT KORB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="4">
        <location>
            <begin position="61"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="140"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI50113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="311" mass="34418" checksum="691BA6FABE3F025B" modified="2017-05-10" version="1">MSAFNAIDDSEDVERDDFTPGIEPQATWCPGCGDFAVLKALKGAMAELGKNPEEVLLVTGIGCSGKLSSYFESYGFHTLHGRALPIARAAKLANPDLEVIAAGGDGDGYGIGGNHFAHTARENHDITYIVFNNEIFGLTKGQTSPTSPKGHKSKTQPHGSAKDPIRPLSMGLTSGASYVSRTAAVNPNQAQEILVEAIEHNGFSHIDFLTQCPTWNKDAKQYVPYIDVQQSDEYGFDHTDRREASEMMYEAESALYEGEVLTGRFYHDDDRLSYGEEKRAVGEMPERPLAEQYHEDDYEWERSYDLLDAHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY33</accession>
    <name>A0A1U7EY33_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Acyl-CoA dehydrogenase</fullName>
            <ecNumber evidence="10">1.3.8.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">acd10</name>
        <name evidence="10" type="ordered locus">NP_4050A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.-" evidence="10"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323732.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY33"/>
    <dbReference type="STRING" id="348780.NP_4050A"/>
    <dbReference type="EnsemblBacteria" id="CAI50116">
        <property type="protein sequence ID" value="CAI50116"/>
        <property type="gene ID" value="NP_4050A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702474"/>
    <dbReference type="KEGG" id="nph:NP_4050A"/>
    <dbReference type="eggNOG" id="arCOG01707">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_3_5_2"/>
    <dbReference type="OrthoDB" id="275197at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00567">
        <property type="entry name" value="ACAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000001">
        <property type="entry name" value="Acyl-CoA dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000002">
        <property type="entry name" value="Acyl-CoA dehydrogenase fadE12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF12">
        <property type="entry name" value="ISOVALERYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016578">
        <property type="entry name" value="HsaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0274">FAD</keyword>
    <keyword evidence="3 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 6 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="9">
        <location>
            <begin position="11"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="8">
        <location>
            <begin position="126"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="7">
        <location>
            <begin position="234"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI50116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="384" mass="42258" checksum="9084F2DCF925127B" modified="2017-05-10" version="1">MELLDNSVVPEHAHDIKQEAREFAAEHIAPNAADYYESGEYPWDVLEAGMDAGLVAQDISEEYGGRGLDLHEILAMAEEFYRADAGIALTLQLASFGAQILQTHGSDDQKETFLRPVAENDQLTGLAVSEPDTGSDLTGMETTAEKDGDEWVLNGEKYWVGNGVEADWVTLYARTGDNPDDPYGNFSLFVVPTDTDGYDAEHIPEKMGFRASKQAHIELNGARIPEDHLIGVEGAGFYMLAEFFNHGRVVVGGHGLGLAAAAIEETADFVHDRTAFGRDVAGFQKVQHILADMMTEFQAARTLNWQAATRVEENDNAGFWAAMAKVKSTETANDCAERGMQLHGGRSVLTKNRISRVYRDVRIPVIYEGANEIQRNLIYGQGPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY38</accession>
    <name>A0A1U7EY38_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Superoxide dismutase</fullName>
            <ecNumber evidence="4 10">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">sod</name>
        <name evidence="13" type="ordered locus">NP_4040A</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Destroys radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the iron/manganese superoxide dismutase family.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="4 10"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323727.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY38"/>
    <dbReference type="STRING" id="348780.NP_4040A"/>
    <dbReference type="EnsemblBacteria" id="CAI50111">
        <property type="protein sequence ID" value="CAI50111"/>
        <property type="gene ID" value="NP_4040A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703210"/>
    <dbReference type="KEGG" id="nph:NP_4040A"/>
    <dbReference type="eggNOG" id="arCOG04147">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031625_2_1_2"/>
    <dbReference type="OrthoDB" id="32917at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.55.40.20:FF:000004">
        <property type="entry name" value="Superoxide dismutase [Fe]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.990">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.40.20">
        <property type="entry name" value="Iron/manganese superoxide dismutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050265">
        <property type="entry name" value="Fe/Mn_Superoxide_Dismutase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001189">
        <property type="entry name" value="Mn/Fe_SOD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019833">
        <property type="entry name" value="Mn/Fe_SOD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019832">
        <property type="entry name" value="Mn/Fe_SOD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019831">
        <property type="entry name" value="Mn/Fe_SOD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036324">
        <property type="entry name" value="Mn/Fe_SOD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036314">
        <property type="entry name" value="SOD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054865">
        <property type="entry name" value="Superox_dis_Halo"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041312">
        <property type="entry name" value="Superox_dis_Halo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11404">
        <property type="entry name" value="SUPEROXIDE DISMUTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11404:SF6">
        <property type="entry name" value="SUPEROXIDE DISMUTASE [MN], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02777">
        <property type="entry name" value="Sod_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00081">
        <property type="entry name" value="Sod_Fe_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000349">
        <property type="entry name" value="SODismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01703">
        <property type="entry name" value="MNSODISMTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54719">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46609">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00088">
        <property type="entry name" value="SOD_MN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Manganese/iron superoxide dismutase N-terminal" evidence="11">
        <location>
            <begin position="8"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Manganese/iron superoxide dismutase C-terminal" evidence="12">
        <location>
            <begin position="94"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000349-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAI50111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="201" mass="22407" checksum="F8B55D27572B32AE" modified="2017-05-10" version="1">MPEHSNPELPPLPYDYDALEPSISEQVVTWHHDTHHQGYVNGLESAEETLAENREAGEFGGSAAAIRNVTHNGSGHYLHTLFWENMDPNGGGEPSGDLADRIEEDFGSYEGWKGEFKAAAGAAGGWALLVYDPVADQLRNLVVDKHDQGALWGSHPILALDVWEHSYYYDYGPDRGEFIDGFFEVVDWDNVAEQYEKATQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY80</accession>
    <name>A0A1U7EY80_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">MTH865 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4192A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323803.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY80"/>
    <dbReference type="STRING" id="348780.NP_4192A"/>
    <dbReference type="EnsemblBacteria" id="CAI50187">
        <property type="protein sequence ID" value="CAI50187"/>
        <property type="gene ID" value="NP_4192A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702000"/>
    <dbReference type="KEGG" id="nph:NP_4192A"/>
    <dbReference type="eggNOG" id="arCOG02797">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_193458_0_0_2"/>
    <dbReference type="OrthoDB" id="335595at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.80">
        <property type="entry name" value="MTH865-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036825">
        <property type="entry name" value="MTH865-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024093">
        <property type="entry name" value="Uncharacterised_MTH865"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07747">
        <property type="entry name" value="MTH865"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69025">
        <property type="entry name" value="Hypothetical protein MTH865"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="83" mass="9105" checksum="AE6C31E38F3AE485" modified="2017-05-10" version="1">MADKDDLREQMIDAFEGADYPINSPMDLVPALPQGPGTKFESGDFSMTAMELNTKLGGGEFPYDSAEAFVDDVIEQLEDQDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY89</accession>
    <name>A0A1U7EY89_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Diadenylate cyclase</fullName>
            <shortName evidence="6">DAC</shortName>
            <ecNumber evidence="6">2.7.7.85</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Cyclic-di-AMP synthase</fullName>
            <shortName evidence="6">c-di-AMP synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">dacZ</name>
        <name evidence="8" type="ordered locus">NP_4194A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Diadenylate cyclase that catalyzes the condensation of 2 ATP molecules into cyclic di-AMP (c-di-AMP). c-di-AMP is a second messenger for intracellular signal transduction involved in the control of important regulatory processes such as osmoregulation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>2 ATP = 3',3'-c-di-AMP + 2 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:35655"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:71500"/>
            <dbReference type="EC" id="2.7.7.85"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the adenylate cyclase family. DacZ subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.85" evidence="6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323804.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY89"/>
    <dbReference type="STRING" id="348780.NP_4194A"/>
    <dbReference type="EnsemblBacteria" id="CAI50188">
        <property type="protein sequence ID" value="CAI50188"/>
        <property type="gene ID" value="NP_4194A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702001"/>
    <dbReference type="KEGG" id="nph:NP_4194A"/>
    <dbReference type="eggNOG" id="arCOG04453">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063222_0_0_2"/>
    <dbReference type="OrthoDB" id="85944at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106408">
        <property type="term" value="F:diadenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1700.10">
        <property type="entry name" value="DNA integrity scanning protein, DisA, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00840">
        <property type="entry name" value="DacZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014499">
        <property type="entry name" value="DAC_DacZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045586">
        <property type="entry name" value="DacZ_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053542">
        <property type="entry name" value="Diadenylate_Cyclase_DacZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050338">
        <property type="entry name" value="DisA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036888">
        <property type="entry name" value="DNA_integrity_DisA_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003390">
        <property type="entry name" value="DNA_integrity_scan_DisA_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041371">
        <property type="entry name" value="Diadnylcyc_Halo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34185">
        <property type="entry name" value="DIADENYLATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34185:SF1">
        <property type="entry name" value="DIADENYLATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02457">
        <property type="entry name" value="DAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19294">
        <property type="entry name" value="DACZ_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019073">
        <property type="entry name" value="UCP019073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143597">
        <property type="entry name" value="YojJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51794">
        <property type="entry name" value="DAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAC" evidence="7">
        <location>
            <begin position="107"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI50188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="267" mass="28912" checksum="BAF14A041F46DE57" modified="2017-05-10" version="1">MSELSDLLDDIVADVDGVFLFSPSGSFYERFGESDPVVVAPENDVGADRYVELPLAFKDVSERIRFGIEGGLEHEFVAEGDQVACAVALFDDDIDTVTRVRAGDFDQSGVYDLFVNSRAEPEVIRNVFEVAIELGKKGQKGKRVGALFVVGDAGKVMNKSRPLSYNPFEKSHVHVGDPIVDVMLKEFSRLDGAFVISDSGKIVSAYRYLEPSAEGVDIPKGLGARHMAAGAITRDTNAVAVVLSESDGLVRAFSGGEIILELDPEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYB9</accession>
    <name>A0A1U7EYB9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">HTH-10 family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_4286A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323850.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYB9"/>
    <dbReference type="STRING" id="348780.NP_4286A"/>
    <dbReference type="EnsemblBacteria" id="CAI50234">
        <property type="protein sequence ID" value="CAI50234"/>
        <property type="gene ID" value="NP_4286A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702021"/>
    <dbReference type="KEGG" id="nph:NP_4286A"/>
    <dbReference type="eggNOG" id="arCOG02272">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101251_0_0_2"/>
    <dbReference type="OrthoDB" id="51502at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007050">
        <property type="entry name" value="HTH_bacterioopsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236:SF1">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04967">
        <property type="entry name" value="HTH_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH bat-type" evidence="3">
        <location>
            <begin position="150"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="218" mass="24219" checksum="4E1323A7355C70C7" modified="2017-05-10" version="1">MPRATLTLTIPEAIWMGELSRTYPETRLRVLSAVKNDEGGVALVELTSPHEEAVISAAREYDSVAAVEVLNDEAEGLLLQLETTMPLLLEPLQRSGVPLEMPFNVQDGEVVWEVTTSRERLSMLGDQLESLGISFTVESIYQQVESEQLLTDHQWDVLSAAVECGYYDTPRSCTQEELADELGIAKSTCSETLHRAEEQIIKRFLSNHEDKSSVAPTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYH8</accession>
    <name>A0A1U7EYH8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4424A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323918.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYH8"/>
    <dbReference type="EnsemblBacteria" id="CAI50303">
        <property type="protein sequence ID" value="CAI50303"/>
        <property type="gene ID" value="NP_4424A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702059"/>
    <dbReference type="KEGG" id="nph:NP_4424A"/>
    <dbReference type="eggNOG" id="arCOG06324">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109224_0_0_2"/>
    <dbReference type="OrthoDB" id="214734at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055808">
        <property type="entry name" value="DUF7384"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24109">
        <property type="entry name" value="DUF7384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="160" mass="16990" checksum="94C761300639F82F" modified="2017-05-10" version="1">MITDGHEPDPTRLVADADVLAADLLVGGPARAALDEWRRHSWLDLVVTAPLCADAAAVIESLADETLAADWRGRLEQEAVVVDQPAGDHPALAAAYRGDGAHILSLDERLQSAEAGANLRGMMSVSVRSPDAFATVFDAAVVYESTFDEPYPGADRDPRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYL3</accession>
    <name>A0A1U7EYL3_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF8112 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4486A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYL3"/>
    <dbReference type="STRING" id="348780.NP_4486A"/>
    <dbReference type="EnsemblBacteria" id="CAI50334">
        <property type="protein sequence ID" value="CAI50334"/>
        <property type="gene ID" value="NP_4486A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_4486A"/>
    <dbReference type="eggNOG" id="arCOG10929">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1318571_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058425">
        <property type="entry name" value="DUF8112"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26417">
        <property type="entry name" value="DUF8112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8112" evidence="1">
        <location>
            <begin position="34"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="208" mass="22645" checksum="797307698347064E" modified="2017-05-10" version="1">MTGDRLFVDCDPGATVTITDRLEGERIPRAELDLAVACDHCGAAIEAGAHVTLYASDTDYGPTDRDGTFDVARTYCAGCDRQSIRLPCRGAVEAVFAATVDDEWRLDGVELRDRSLSDDGVEWKPPAVWRRLVGVDLDAYLQHPKTNGMTAAPEDVYDTLERLGVDLSTFIEDGQLRLEGSAEKAAQETIEAHLSEGAKTVRETVSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYN2</accession>
    <name>A0A1U7EYN2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cold shock protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cspA4</name>
        <name evidence="4" type="ordered locus">NP_4552A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323982.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYN2"/>
    <dbReference type="STRING" id="348780.NP_4552A"/>
    <dbReference type="EnsemblBacteria" id="CAI50367">
        <property type="protein sequence ID" value="CAI50367"/>
        <property type="gene ID" value="NP_4552A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702639"/>
    <dbReference type="KEGG" id="nph:NP_4552A"/>
    <dbReference type="eggNOG" id="arCOG02983">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117621_7_2_2"/>
    <dbReference type="OrthoDB" id="202422at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012156">
        <property type="entry name" value="Cold_shock_CspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050181">
        <property type="entry name" value="Cold_shock_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11544">
        <property type="entry name" value="COLD SHOCK DOMAIN CONTAINING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002599">
        <property type="entry name" value="Cold_shock_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00050">
        <property type="entry name" value="COLDSHOCK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CSD" evidence="3">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="64" mass="7189" checksum="07A9504C80E42B62" modified="2017-05-10" version="1">MAKGEVDFFNDTGGYGFIDTDESDEDVFFHMEDIGGPDLEEGQEVEFDIEQADKGPRAKNLTRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYQ0</accession>
    <name>A0A1U7EYQ0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Disulfide bond formation protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">bdbC</name>
        <name evidence="13" type="ordered locus">NP_4526A</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DsbB family. BdbC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323969.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYQ0"/>
    <dbReference type="STRING" id="348780.NP_4526A"/>
    <dbReference type="EnsemblBacteria" id="CAI50354">
        <property type="protein sequence ID" value="CAI50354"/>
        <property type="gene ID" value="NP_4526A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702549"/>
    <dbReference type="KEGG" id="nph:NP_4526A"/>
    <dbReference type="eggNOG" id="arCOG06168">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128688_0_0_2"/>
    <dbReference type="OrthoDB" id="213767at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1550.10">
        <property type="entry name" value="DsbB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003752">
        <property type="entry name" value="DiS_bond_form_DsbB/BdbC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012187">
        <property type="entry name" value="Disulphide_bond_form_BdbC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023380">
        <property type="entry name" value="DsbB-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43469">
        <property type="entry name" value="DISULFIDE FORMATION PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43469:SF1">
        <property type="entry name" value="SPBETA PROPHAGE-DERIVED DISULFIDE BOND FORMATION PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02600">
        <property type="entry name" value="DsbB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036659">
        <property type="entry name" value="BdbC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158442">
        <property type="entry name" value="DsbB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAI50354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="135" mass="14357" checksum="4D7D8A365F161253" modified="2017-05-10" version="1">MRRRLLAVGTLVAAVATLGSLYFSDVAGFVPCDLCWYQRILMYPLVVVFGVAAVESRVGVWRTALPLSGLGVVVSAYHTAIQLTPETTCTVGGGCTAIYWRGLGVFTIPRLALVAFLLLAALSVALAVVERRVDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYT4</accession>
    <name>A0A1U7EYT4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="13">CopCD domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">NP_4610A</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324011.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYT4"/>
    <dbReference type="STRING" id="348780.NP_4610A"/>
    <dbReference type="EnsemblBacteria" id="CAI50396">
        <property type="protein sequence ID" value="CAI50396"/>
        <property type="gene ID" value="NP_4610A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702115"/>
    <dbReference type="KEGG" id="nph:NP_4610A"/>
    <dbReference type="eggNOG" id="arCOG11365">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_414274_0_0_2"/>
    <dbReference type="OrthoDB" id="206320at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006825">
        <property type="term" value="P:copper ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046688">
        <property type="term" value="P:response to copper ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032694">
        <property type="entry name" value="CopC/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007348">
        <property type="entry name" value="CopC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008457">
        <property type="entry name" value="Cu-R_CopD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014755">
        <property type="entry name" value="Cu-Rt/internalin_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34820">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YEBZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34820:SF4">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YEBZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04234">
        <property type="entry name" value="CopC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05425">
        <property type="entry name" value="CopD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0186">Copper</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="193"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="235"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="268"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="307"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="347"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="381"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="446"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="CopC" evidence="11">
        <location>
            <begin position="27"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Copper resistance protein D" evidence="12">
        <location>
            <begin position="342"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="587"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="606"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF05425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAI50396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="659" mass="67202" checksum="A82AC5D246518667" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYX0</accession>
    <name>A0A1U7EYX0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4682A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324046.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYX0"/>
    <dbReference type="EnsemblBacteria" id="CAI50432">
        <property type="protein sequence ID" value="CAI50432"/>
        <property type="gene ID" value="NP_4682A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702140"/>
    <dbReference type="KEGG" id="nph:NP_4682A"/>
    <dbReference type="eggNOG" id="arCOG06398">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_179746_1_0_2"/>
    <dbReference type="OrthoDB" id="294990at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045397">
        <property type="entry name" value="TumE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20126">
        <property type="entry name" value="TumE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="93" mass="11022" checksum="572C7346D057FE1A" modified="2017-05-10" version="1">MGHTVIEDVEDRVGNRVIRRKIIKTDDPQYPSGYRYALHYGYTDGRDTILRYDNENETVGRHECHTPEGVTEIEFPGMMDLRDRFRKEIEHNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ31</accession>
    <name>A0A1U7EZ31_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Beta-ribofuranosylaminobenzene 5'-phosphate synthase</fullName>
            <shortName evidence="2">Beta-RFA-P synthase</shortName>
            <ecNumber evidence="2">2.4.2.54</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_4930A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the condensation of 4-aminobenzoate (pABA) with 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to produce beta-ribofuranosylaminobenzene 5'-phosphate (beta-RFA-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>5-phospho-alpha-D-ribose 1-diphosphate + 4-hydroxybenzoate + H(+) = 4-(beta-D-ribofuranosyl)phenol 5'-phosphate + CO2 + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:48556"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17879"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:82767"/>
            <dbReference type="EC" id="2.4.2.54"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; 5,6,7,8-tetrahydromethanopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta-RFA-P synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.54" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324168.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ31"/>
    <dbReference type="STRING" id="348780.NP_4930A"/>
    <dbReference type="EnsemblBacteria" id="CAI50556">
        <property type="protein sequence ID" value="CAI50556"/>
        <property type="gene ID" value="NP_4930A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702203"/>
    <dbReference type="KEGG" id="nph:NP_4930A"/>
    <dbReference type="eggNOG" id="arCOG01026">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061764_0_0_2"/>
    <dbReference type="OrthoDB" id="85156at2157"/>
    <dbReference type="UniPathway" id="UPA00065"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043793">
        <property type="term" value="F:beta-ribofuranosylaminobenzene 5'-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004422">
        <property type="entry name" value="RFAP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00144">
        <property type="entry name" value="beta_RFAP_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861:SF6">
        <property type="entry name" value="BETA-RIBOFURANOSYLPHENOL 5'-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861">
        <property type="entry name" value="HOMOSERINE/4-DIPHOSPHOCYTIDYL-2-C-METHYL-D-ERYTHRITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004884">
        <property type="entry name" value="Sugar_kin_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="3">
        <location>
            <begin position="58"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR004884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="323" mass="33737" checksum="B3152BA04E6677EA" modified="2017-05-10" version="1">MRVRVETGGRVHIGFQNLSLAHERLYGGVGLAIDEPRAVVEAEPASTVECDDAVVATYAERACSELGVNGARVEVCERLPRHVGLGSGTQLALATLTAVARAHDVALTVRKRAPALGRGGRSGVGVATFEAGGFVVDAGHPTERFTTDPPAAGSWTVPEVVARRRVPDDWRLLLVLPDAAPGRSGDDEDESIRAVVEDADPGVADELAGVLVRRLLPAVAEGRLDAFGDAVGAFGRLNGAWYADQQGGVYRPPAGRIIDRLSDCPAVRGLGQSSWGPAVYGVTDASMAADATADARAALSELGVDARVQVVEPRNEGARVETL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ78</accession>
    <name>A0A1U7EZ78_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Iron-sulfur protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">NP_4970A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50576.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939735.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ78"/>
    <dbReference type="SMR" id="A0A1U7EZ78"/>
    <dbReference type="STRING" id="348780.NP_4970A"/>
    <dbReference type="EnsemblBacteria" id="CAI50576">
        <property type="protein sequence ID" value="CAI50576"/>
        <property type="gene ID" value="NP_4970A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702216"/>
    <dbReference type="KEGG" id="nph:NP_4970A"/>
    <dbReference type="eggNOG" id="arCOG02237">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_388139_0_0_2"/>
    <dbReference type="OrthoDB" id="23478at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050572">
        <property type="entry name" value="Fe-S_Ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003813">
        <property type="entry name" value="MvhD/FlpD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687">
        <property type="entry name" value="ADENYLYLSULFATE REDUCTASE, BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687:SF4">
        <property type="entry name" value="BLR5484 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02662">
        <property type="entry name" value="FlpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="311"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="331"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="603"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI50576.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="711" mass="74987" checksum="A3415E9A025C9CB9" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ98</accession>
    <name>A0A1U7EZ98_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Aldehyde ferredoxin oxidoreductase</fullName>
            <ecNumber evidence="11">1.2.7.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">aor4</name>
        <name evidence="11" type="ordered locus">NP_5016A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>tungstopterin</name>
            <dbReference type="ChEBI" id="CHEBI:30402"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AOR/FOR family.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.5" evidence="11"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324209.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ98"/>
    <dbReference type="STRING" id="348780.NP_5016A"/>
    <dbReference type="EnsemblBacteria" id="CAI50599">
        <property type="protein sequence ID" value="CAI50599"/>
        <property type="gene ID" value="NP_5016A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702509"/>
    <dbReference type="KEGG" id="nph:NP_5016A"/>
    <dbReference type="eggNOG" id="arCOG00706">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020364_1_0_2"/>
    <dbReference type="OrthoDB" id="30771at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033726">
        <property type="term" value="F:aldehyde ferredoxin oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.569.10">
        <property type="entry name" value="Aldehyde Ferredoxin Oxidoreductase Protein, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.599.10">
        <property type="entry name" value="Aldehyde Ferredoxin Oxidoreductase Protein, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.9.10">
        <property type="entry name" value="Aldehyde ferredoxin oxidoreductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013984">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013985">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013983">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036503">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001203">
        <property type="entry name" value="OxRdtase_Ald_Fedxn_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036021">
        <property type="entry name" value="Tungsten_al_ferr_oxy-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051919">
        <property type="entry name" value="W-dependent_AOR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30038">
        <property type="entry name" value="ALDEHYDE FERREDOXIN OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30038:SF0">
        <property type="entry name" value="TUNGSTEN-CONTAINING ALDEHYDE FERREDOXIN OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01314">
        <property type="entry name" value="AFOR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02730">
        <property type="entry name" value="AFOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00790">
        <property type="entry name" value="AFOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48310">
        <property type="entry name" value="Aldehyde ferredoxin oxidoreductase, C-terminal domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56228">
        <property type="entry name" value="Aldehyde ferredoxin oxidoreductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde ferredoxin oxidoreductase N-terminal" evidence="10">
        <location>
            <begin position="7"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="625"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI50599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="646" mass="69600" checksum="8FDD6A0EE66632E5" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZH0</accession>
    <name>A0A1U7EZH0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ribonuclease H, type 1</fullName>
            <ecNumber evidence="2">3.1.26.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rnhA2</name>
        <name evidence="2" type="ordered locus">NP_5188A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.26.4" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324295.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZH0"/>
    <dbReference type="STRING" id="348780.NP_5188A"/>
    <dbReference type="DNASU" id="3703110"/>
    <dbReference type="EnsemblBacteria" id="CAI50685">
        <property type="protein sequence ID" value="CAI50685"/>
        <property type="gene ID" value="NP_5188A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703110"/>
    <dbReference type="KEGG" id="nph:NP_5188A"/>
    <dbReference type="eggNOG" id="arCOG02942">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1375506_0_0_2"/>
    <dbReference type="OrthoDB" id="52651at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd09279">
        <property type="entry name" value="RNase_HI_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049863">
        <property type="entry name" value="RnhA-like_halobact"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041306">
        <property type="entry name" value="RnaseHI_Halo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46387">
        <property type="entry name" value="POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46387:SF2">
        <property type="entry name" value="RIBONUCLEASE HI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13456">
        <property type="entry name" value="RVT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-1" evidence="1">
        <location>
            <begin position="64"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="195" mass="21358" checksum="DF97E4D78D0DC998" modified="2017-05-10" version="1">MPVIECDPERARERLVEAGVEVEPGNTDHERWRTEYAGGTAVAYDDKVVLQGDSARLAGLLQDGGGRAHIYFDGASRGNPGPAAIGWVIVTDDGIVAEGSERIGRATNNQAEYEALIEALAAAADYAFDEAVVKGDSQLIVKQVRGEWDTNDPTLREKRVRVRKLLEEFSEWSLDHVPREINDRADDLANEALDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZJ1</accession>
    <name>A0A1U7EZJ1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_5276A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324338.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZJ1"/>
    <dbReference type="STRING" id="348780.NP_5276A"/>
    <dbReference type="EnsemblBacteria" id="CAI50729">
        <property type="protein sequence ID" value="CAI50729"/>
        <property type="gene ID" value="NP_5276A"/>
    </dbReference>
    <dbReference type="GeneID" id="54763334"/>
    <dbReference type="KEGG" id="nph:NP_5276A"/>
    <dbReference type="eggNOG" id="arCOG07553">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3039159_0_0_2"/>
    <dbReference type="OrthoDB" id="260782at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055755">
        <property type="entry name" value="DUF7331"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24018">
        <property type="entry name" value="DUF7331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="54" mass="5762" checksum="C12AD9F5727FA266" modified="2017-05-10" version="1">MSAPPQIDDGDRGVDNGERCADIVLETGEVVIYDTDNHTAWVQSDTAVARDAAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZJ8</accession>
    <name>A0A1U7EZJ8_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Putative [LysW]-aminoadipate semialdehyde/glutamate semialdehyde transaminase</fullName>
            <ecNumber evidence="6">2.6.1.118</ecNumber>
            <ecNumber evidence="6">2.6.1.124</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">argD</name>
        <name evidence="6" type="synonym">lysJ</name>
        <name evidence="8" type="ordered locus">NP_5264A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in both the arginine and lysine biosynthetic pathways.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[amino-group carrier protein]-C-terminal-gamma-(L-lysyl)-L-glutamate + 2-oxoglutarate = [amino-group carrier protein]-C-terminal-N-(1-carboxy-5-oxopentan-1-yl)-L-glutamine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:41952"/>
            <dbReference type="Rhea" id="RHEA-COMP:9714"/>
            <dbReference type="Rhea" id="RHEA-COMP:9715"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:78501"/>
            <dbReference type="ChEBI" id="CHEBI:78526"/>
            <dbReference type="EC" id="2.6.1.118"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[amino-group carrier protein]-C-terminal-gamma-(L-ornithyl)-L-glutamate + 2-oxoglutarate = [amino-group carrier protein]-C-terminal-gamma-(L-glutamyl-5-semialdehyde)-L-glutamate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:52672"/>
            <dbReference type="Rhea" id="RHEA-COMP:13327"/>
            <dbReference type="Rhea" id="RHEA-COMP:13328"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:136761"/>
            <dbReference type="ChEBI" id="CHEBI:136763"/>
            <dbReference type="EC" id="2.6.1.124"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
        <text evidence="6">Binds 1 pyridoxal phosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid biosynthesis; L-arginine biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (Thermus route): step 4/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. LysJ subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.118" evidence="6"/>
    <dbReference type="EC" id="2.6.1.124" evidence="6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324332.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZJ8"/>
    <dbReference type="STRING" id="348780.NP_5264A"/>
    <dbReference type="EnsemblBacteria" id="CAI50723">
        <property type="protein sequence ID" value="CAI50723"/>
        <property type="gene ID" value="NP_5264A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702518"/>
    <dbReference type="KEGG" id="nph:NP_5264A"/>
    <dbReference type="eggNOG" id="arCOG00914">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_10_0_2"/>
    <dbReference type="OrthoDB" id="85346at2157"/>
    <dbReference type="UniPathway" id="UPA00033">
        <property type="reaction ID" value="UER00038"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00068"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000004">
        <property type="entry name" value="Acetylornithine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02084">
        <property type="entry name" value="LysJ_aminotrans_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004636">
        <property type="entry name" value="AcOrn/SuccOrn_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050103">
        <property type="entry name" value="Class-III_PLP-dep_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037537">
        <property type="entry name" value="LysJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00707">
        <property type="entry name" value="argD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986:SF79">
        <property type="entry name" value="ACETYLORNITHINE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986">
        <property type="entry name" value="AMINOTRANSFERASE CLASS III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 6 8" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="5 6 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6 8" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="95"/>
            <end position="96"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="207"/>
            <end position="210"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="6">
        <location>
            <position position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI50723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="375" mass="39348" checksum="DCF5B5BE6596B702" modified="2017-05-10" version="1">MSGFVFSEKPIGIERGEGVYLYDTKGNEYLDFGASYACTPVGHCHPDVVAATTEQLERLMYVQASYPNAPRTALYEQLATVAPGDIDNVWLCNSGTEANEAALKFARHATGREKIVATMQGFHGRTMGTLAATWKDKYRDGFEPLAGGFEFVPYGDAEAMADAVDEDTAAVIVEPVQGEGGINPAPNGYLQSVRETTESAGAAMVLDEIQTGLGRTGTLWAAEEYGVVPDILTTAKGLGSGLPIGATLVRDWVAADCGNHGSTFSGGPVTTAAAGATLDVITDEDLPSHAAELGTYLVEALEAAIGDEVREVRGNGLMVGVEVKRGANPILKELALNHDILALPAGRSVVRLLPPLVIDRGDADAVVDALEAIIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZK9</accession>
    <name>A0A1U7EZK9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5312A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <lineage>
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            <taxon>Natronomonas</taxon>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
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            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
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        <property type="taxonomic scope" value="Archaea"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Photosystem II 12 kDa extrinsic protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="rSAM_horseshoe"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
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        <location>
            <begin position="184"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="572" mass="62532" checksum="DED4C0336FAA5B1E" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZQ1</accession>
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    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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            <authorList>
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
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            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
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        <property type="gene ID" value="NP_5368A"/>
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        <property type="component" value="Chromosome"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="INTEGRASE_RECOMBINASE PROTEIN MJ0367-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30349">
        <property type="entry name" value="PHAGE INTEGRASE-RELATED"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF02899">
        <property type="entry name" value="Phage_int_SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="8"/>
            <end position="97"/>
        </location>
    </feature>
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        <location>
            <begin position="121"/>
            <end position="343"/>
        </location>
    </feature>
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        <location>
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            <end position="297"/>
        </location>
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        <location>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI50775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="349" mass="39890" checksum="B68CDC887DE72B9C" modified="2017-05-10" version="1">MSDDLEPIAPAEAVEMYIEARQDDCTENTIEGQYYRLQAFLAWCDEEDITNLNELDGRDLYAYRVWRREGGYSDTELAGATLRGDLATLRAFLRFCGEVEAVPPEFFDRVPLPSVSGGADVSASTLDPDRAQAILEYLQQFEYASKRHVIVLLLWHAGCRVGALRALDVDDLDLAGDRPNATGPGIKFVHRPDEGTPLKNKRKSERWNTISEGVANVIEDYIASRRTEAEDDYGRRPLISTRYGRMSRSAIRQELYRVTRPCWYNDGCPHDRDPDECEATDDGSMSKCPSSRSPHDVRSGRLTFYRLREVDEKVVSDRMDASEEILDKHYDRRSERQKAEQRRSHLPDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILR8</accession>
    <name>Q3ILR8_NATPD</name>
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        <recommendedName>
            <fullName evidence="4">Small CPxCG-related zinc finger protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="1" type="ordered locus">NP_3250A</name>
        <name evidence="2" type="ordered locus">NP_7074A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
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        <lineage>
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            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
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        <name>PL23</name>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
            <strain evidence="2">Gabara</strain>
            <plasmid evidence="2">PL23</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="CAI50953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILR8"/>
    <dbReference type="STRING" id="348780.NP_3250A"/>
    <dbReference type="EnsemblBacteria" id="CAI49716">
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        <property type="gene ID" value="NP_3250A"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI50953">
        <property type="protein sequence ID" value="CAI50953"/>
        <property type="gene ID" value="NP_7074A"/>
    </dbReference>
    <dbReference type="GeneID" id="77383471"/>
    <dbReference type="KEGG" id="nph:NP_3250A"/>
    <dbReference type="KEGG" id="nph:NP_7074A"/>
    <dbReference type="HOGENOM" id="CLU_3263903_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="41" mass="4545" checksum="89BF12027B396DF5" modified="2005-11-08" version="1">MSTTAEPVTVTCGLYDAEFEVIVDDKSDIQHCPRCGRSVDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILT5</accession>
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        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3314A</name>
        <name evidence="4" type="ordered locus">NP_7038A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
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            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
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        <name>PL23</name>
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    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
            <strain evidence="4">Gabara</strain>
            <plasmid evidence="4">PL23</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CR936259">
        <property type="protein sequence ID" value="CAI50935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323369.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILT5"/>
    <dbReference type="STRING" id="348780.NP_3314A"/>
    <dbReference type="EnsemblBacteria" id="CAI49748">
        <property type="protein sequence ID" value="CAI49748"/>
        <property type="gene ID" value="NP_3314A"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI50935">
        <property type="protein sequence ID" value="CAI50935"/>
        <property type="gene ID" value="NP_7038A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703479"/>
    <dbReference type="KEGG" id="nph:NP_3314A"/>
    <dbReference type="KEGG" id="nph:NP_7038A"/>
    <dbReference type="HOGENOM" id="CLU_1881140_0_0_2"/>
    <dbReference type="OrthoDB" id="289957at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="135" mass="14010" checksum="EE11EDAA4DCA00B9" modified="2005-11-08" version="1">MRTIGVVAFVLFLGFSVSMLSMSGIGPAIFGQDTDDEVGLGPQEDLEQAADDASVDEGDEGGIGADVAAEDEPTTIGFVLEGAQFAVGLVASIAALPFTLMNLGLPRFFAIPLGMVLQIVGAVGLFQFLTGREWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILW3</accession>
    <name>Q3ILW3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_6254A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324504.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILW3"/>
    <dbReference type="EnsemblBacteria" id="CAI50907">
        <property type="protein sequence ID" value="CAI50907"/>
        <property type="gene ID" value="NP_6254A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694657"/>
    <dbReference type="KEGG" id="nph:NP_6254A"/>
    <dbReference type="HOGENOM" id="CLU_1202649_0_0_2"/>
    <dbReference type="OrthoDB" id="206010at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="230" mass="24657" checksum="CF3747EEDC660A25" modified="2005-11-08" version="1">MERRAFLMATGGAATIAGCTTGGNTEEENGDVEYADDREASIEIADSTVIGASDDNVADPWLQVDVENGTDVPHGGIRIESEVLDDDGTVLASNEHITSYLPPETTLRYFHEGAFEMDEFDSLEHDVTDAHAQVRSTKLEDITVTNTSLSAGGDLINVVGDLDLADSDHDRVVVIAPIWDEAGRLRGTGTYIENSPTESFEFSADSSGFRAPGGAEPIDSYDVYAFDGFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM03</accession>
    <name>Q3IM03_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_6172A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324464.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM03"/>
    <dbReference type="EnsemblBacteria" id="CAI50866">
        <property type="protein sequence ID" value="CAI50866"/>
        <property type="gene ID" value="NP_6172A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694635"/>
    <dbReference type="KEGG" id="nph:NP_6172A"/>
    <dbReference type="HOGENOM" id="CLU_142037_0_0_2"/>
    <dbReference type="OrthoDB" id="205962at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058276">
        <property type="entry name" value="DUF7970"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25925">
        <property type="entry name" value="DUF7970"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="56"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="149" mass="15873" checksum="639F9811E4D0A897" modified="2005-11-08" version="1">MGGMKEGAGSDPFADDADVDADAETDTSTDAGADPTPEAGAGTDTDPLSSTETDPSAATTGGAATSGQSRGQPYIHVRDKVKEGREDHPVPLRAHNRDRIADLQQAVEAELGEDVYKSDLLEAAIEHAIENPQDIAEMLETERYGYNWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM05</accession>
    <name>Q3IM05_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ribonucleoside-diphosphate reductase beta subunit</fullName>
            <ecNumber evidence="2">1.17.4.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">nrdB1</name>
        <name evidence="2" type="ordered locus">NP_6166A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribonucleoside diphosphate reductase small chain family.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="2"/>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324462.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM05"/>
    <dbReference type="EnsemblBacteria" id="CAI50863">
        <property type="protein sequence ID" value="CAI50863"/>
        <property type="gene ID" value="NP_6166A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694598"/>
    <dbReference type="KEGG" id="nph:NP_6166A"/>
    <dbReference type="HOGENOM" id="CLU_053322_0_0_2"/>
    <dbReference type="OrthoDB" id="209677at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009263">
        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01049">
        <property type="entry name" value="RNRR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.620.20">
        <property type="entry name" value="Ribonucleotide Reductase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012348">
        <property type="entry name" value="RNR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033909">
        <property type="entry name" value="RNR_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000358">
        <property type="entry name" value="RNR_small_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00268">
        <property type="entry name" value="Ribonuc_red_sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="336" mass="39319" checksum="71934B06D24C55C7" modified="2005-11-08" version="1">MTESKTTGRDTETDIFSERTQLKPYEYADFLDYKDAIRNSYWVHTEFNFSGDVQDFRTNTTPAEKTVIKRTMLAIAQIEVQVKTFWADIYDEMPKTEVGNVGMTFAESEVRHMDAYSHLLDILGITEDFEEVTDVPAIEDRIEYLDKYLEKSESDDKQEYVMSILLFSTFVEHVSLFSQFLIMMSFDKYKKKFKGIANAVEATSKEEQIHGLFGVELVETIREENPDLFDEDFEEEVQAACQRAFEAEMKILDWIFGEGELEFLPRTHVDAFLRDRFNQSLENVGVEPIFEPDDGLLEETRWFDEDIMMTKDNDFFSKRSTTYNKHAQSVTAEDMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM62</accession>
    <name>Q3IM62_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="14">SSSF family transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">tp09</name>
        <name evidence="14" type="ordered locus">NP_6036A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="14 15">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324405.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM62"/>
    <dbReference type="EnsemblBacteria" id="CAI50798">
        <property type="protein sequence ID" value="CAI50798"/>
        <property type="gene ID" value="NP_6036A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694604"/>
    <dbReference type="KEGG" id="nph:NP_6036A"/>
    <dbReference type="HOGENOM" id="CLU_018808_15_2_2"/>
    <dbReference type="OrthoDB" id="9779at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046942">
        <property type="term" value="P:carboxylic acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018212">
        <property type="entry name" value="Na/solute_symporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050277">
        <property type="entry name" value="Sodium:Solute_Symporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48086">
        <property type="entry name" value="SODIUM/PROLINE SYMPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48086:SF3">
        <property type="entry name" value="SODIUM_PROLINE SYMPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00456">
        <property type="entry name" value="NA_SOLUT_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0614">Plasmid</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="11" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="145"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="243"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="282"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="336"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="380"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="405"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="441"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="467"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAI50798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="504" mass="52668" checksum="473B3D32C5096514" modified="2005-11-08" version="1">MMDLVVPGFVALFILSVFGVGLYSKRLIDSSDEFYGATKMFGAAVITLASMSGIMSAFGFIGGPGLVYEMGTSSLWMTFASGLGFAMAYWIIGKRVRGMADVADIGTLGDIADERFNSGAIRAMLALILFIACWAYLASQVAGGGYVLSVILGISIEAAVWLVFGLIILYVAVGGMASAQLAGAYTGAVMLIGVVGVVVGFFQIAGGMGSTTVTVVEAGELTGEDVTKAFTPQLLDGWGLAEGAAPGLLLIWPIVFSIGVMGQPQVLQRMFSIDDPKGLRTVGLVSGVTYAVGSLLWMLIGFVALYMVATGTIEPLVDPDMAAFEFVERLHIVLQMIIYAGLVAAIMSTAAFFLSVASGAIARDFVRAMGWKVSERRETWLGRAAVLGVGVGSVLFGLYGGHLVAILGTFGWGTFVSGTVPAVVVGLLWKGASREGVTAGLASALILNITLLAATQLGFTFPIGMDFYFIAIAVSISVTIFVSFLTDGASGENVPEHVKPIFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMH6</accession>
    <name>Q3IMH6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA repair protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">nreA</name>
        <name evidence="4" type="ordered locus">NP_5182A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in DNA damage repair.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Nre family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324292.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMH6"/>
    <dbReference type="STRING" id="348780.NP_5182A"/>
    <dbReference type="EnsemblBacteria" id="CAI50682">
        <property type="protein sequence ID" value="CAI50682"/>
        <property type="gene ID" value="NP_5182A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702285"/>
    <dbReference type="KEGG" id="nph:NP_5182A"/>
    <dbReference type="eggNOG" id="arCOG04269">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039703_0_0_2"/>
    <dbReference type="OrthoDB" id="6609at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02096">
        <property type="entry name" value="Nre"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033167">
        <property type="entry name" value="Nre"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006979">
        <property type="entry name" value="Nre_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053546">
        <property type="entry name" value="Nre_DNA_repair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006978">
        <property type="entry name" value="Nre_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041387">
        <property type="entry name" value="DNArepr_NreA_Halo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38136">
        <property type="entry name" value="DNA REPAIR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38136:SF2">
        <property type="entry name" value="DNA REPAIR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04895">
        <property type="entry name" value="Nre_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04894">
        <property type="entry name" value="Nre_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="1" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Archaeal Nre N-terminal" evidence="2">
        <location>
            <begin position="21"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="Archaeal Nre C-terminal" evidence="3">
        <location>
            <begin position="311"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="429" mass="47422" checksum="F920CCF5366678D6" modified="2005-11-08" version="1">MRLDEYVDDIEQDDSVALRKLAEEKSYAVTEYIDDARTRFDETLTDDTLVGSTAPSVFVGRSSYPNVSAGVLAPVGDENDAEAYATTGEWYQQGFSIDDVFERRTGLLNSTRRSNVDVYDVWDGFVGTQREVALADDPVDIELGLDDRPGFDLDGPLEDIAAPRGPRAFAESARVTENPSVPRAIEKTLSDDDWRAEGAITYLYRRGFDVYDINNVLSVGALGEAENRRLVPTRWSITAVDDTVGQYLRGTIRNAPPIDRTLVWHTSYMGNDYWVVAAPGDWEFELLELKAPGSIWNPDPDGQLYMAADNEGYDGRSGYVEETSGAYYAARLGVLEALADRDRQAKVFVVRYATEAYWAPVGVWQIRESVRDAFDGEPAVAETFHDAVRQLVPQLPVSMAALRRKSTMAAGVQADLSAFGAGGDSSRSR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMP5</accession>
    <name>SYH_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Histidine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Histidyl-tRNA synthetase</fullName>
            <shortName evidence="1">HisRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisS</name>
        <name type="ordered locus">NP_5044A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(His) + L-histidine + ATP = L-histidyl-tRNA(His) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17313"/>
            <dbReference type="Rhea" id="RHEA-COMP:9665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78527"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.21"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.21" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324223.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMP5"/>
    <dbReference type="SMR" id="Q3IMP5"/>
    <dbReference type="STRING" id="348780.NP_5044A"/>
    <dbReference type="EnsemblBacteria" id="CAI50613">
        <property type="protein sequence ID" value="CAI50613"/>
        <property type="gene ID" value="NP_5044A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702823"/>
    <dbReference type="KEGG" id="nph:NP_5044A"/>
    <dbReference type="eggNOG" id="arCOG00404">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025113_3_1_2"/>
    <dbReference type="OrthoDB" id="8659at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004821">
        <property type="term" value="F:histidine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006427">
        <property type="term" value="P:histidyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00773">
        <property type="entry name" value="HisRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00859">
        <property type="entry name" value="HisRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00127">
        <property type="entry name" value="His_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015807">
        <property type="entry name" value="His-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004516">
        <property type="entry name" value="HisRS/HisZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033656">
        <property type="entry name" value="HisRS_anticodon"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00442">
        <property type="entry name" value="hisS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43707:SF1">
        <property type="entry name" value="HISTIDINE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43707">
        <property type="entry name" value="HISTIDYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001549">
        <property type="entry name" value="His-tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000016400" description="Histidine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="412"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="422"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="432" mass="47682" checksum="43AB7C88C0308D3F" modified="2005-11-08" version="1">MYDGLKGFREFYPGEMSARREVTDTIETRARRYGFREVGTPALERTQLYVDKSGEEIVEELYAFEDKGGREVSLTPELTPTVARMVVAKQQALAKPVKWFSTRPFWRYEQVQQGRFREFYQTNIDIFGSERPEADAEVLATAADALTDLGLDGADFEFRVSHRDILGGLLAAMDSDVDTTDAIRAVDKREKIDDEEYRSLLVGAGLTHEEADTFDSLLRTDDLSELVEFAGTDRVEAAVENLQAVLDAAADFGVREFCSVSLETARGLDYYTGVVFECFDTSGEVSRSVFGGGRYDDLIESFGGQPTPAVGVGIGHETLSLLCQRAGVWPAEELSTDYYVLTVGDTRDVALDVARELRALGHVVETDLSDRGFGDQLSYADGINAETVVVVGEQDLEDDAVTLKDMASGDQTQVPLAAFPPEEGRPTYDDYA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMX8</accession>
    <name>RL14_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl14</name>
        <name type="ordered locus">NP_4874A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to 23S rRNA. Forms part of two intersubunit bridges in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Forms a cluster with proteins L3 and L24e, part of which may contact the 16S rRNA in 2 intersubunit bridges.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324140.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMX8"/>
    <dbReference type="SMR" id="Q3IMX8"/>
    <dbReference type="STRING" id="348780.NP_4874A"/>
    <dbReference type="EnsemblBacteria" id="CAI50528">
        <property type="protein sequence ID" value="CAI50528"/>
        <property type="gene ID" value="NP_4874A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703124"/>
    <dbReference type="KEGG" id="nph:NP_4874A"/>
    <dbReference type="eggNOG" id="arCOG04095">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095071_3_0_2"/>
    <dbReference type="OrthoDB" id="23569at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00337">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.150.20:FF:000007">
        <property type="entry name" value="50S ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.150.20">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01367">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000218">
        <property type="entry name" value="Ribosomal_uL14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019971">
        <property type="entry name" value="Ribosomal_uL14_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019972">
        <property type="entry name" value="Ribosomal_uL14_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036853">
        <property type="entry name" value="Ribosomal_uL14_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006344">
        <property type="entry name" value="PRK08571.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03673">
        <property type="entry name" value="uL14_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761">
        <property type="entry name" value="50S/60S RIBOSOMAL PROTEIN L14/L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761:SF8">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00238">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01374">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50193">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00049">
        <property type="entry name" value="RIBOSOMAL_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000266609" description="Large ribosomal subunit protein uL14">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="132" mass="14243" checksum="F8E436CCE6BE6FEE" modified="2005-11-08" version="1">MEALNADVTQGLEKGSLITCADNTGARELKVTSISGYSGTKNRHPKAGLGDKITVSVTKGTPEMRRQVLEAVVIRQRKPIRRPDGTRVKFEDNAAVIVDENEDPRGTELKGPIAREVAERFGSIASTATIIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IN37</accession>
    <name>Q3IN37_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">GTP cyclohydrolase 1 domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_4756A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP cyclohydrolase I type 2/NIF3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324081.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IN37"/>
    <dbReference type="STRING" id="348780.NP_4756A"/>
    <dbReference type="EnsemblBacteria" id="CAI50469">
        <property type="protein sequence ID" value="CAI50469"/>
        <property type="gene ID" value="NP_4756A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702167"/>
    <dbReference type="KEGG" id="nph:NP_4756A"/>
    <dbReference type="eggNOG" id="arCOG04454">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037423_3_0_2"/>
    <dbReference type="OrthoDB" id="85198at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1390.30:FF:000001">
        <property type="entry name" value="GTP cyclohydrolase 1 type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1390.30">
        <property type="entry name" value="NIF3 (NGG1p interacting factor 3)-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002678">
        <property type="entry name" value="DUF34/NIF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036069">
        <property type="entry name" value="DUF34/NIF3_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00486">
        <property type="entry name" value="YbgI_SA1388"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13799:SF14">
        <property type="entry name" value="GTP CYCLOHYDROLASE 1 TYPE 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13799">
        <property type="entry name" value="NGG1 INTERACTING FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01784">
        <property type="entry name" value="DUF34_NIF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102705">
        <property type="entry name" value="NIF3 (NGG1p interacting factor 3)-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR602678-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="254" mass="27134" checksum="33EF58E015FF2D83" modified="2005-11-08" version="1">MQLETLCSRLDDRLNHDDYADVDASANGLQVGDDDRTVERVAVAVDGVEATVDAAAEAGADLLVVHHGFVWGGLDRVTGREYDRIAACIENDVPLYVSHLPLDGHPELGNAAGLAEFLDCTVTEPFGDYGGVTIGQQAQAETPYTVATLRERLGDLDTGGQPVQVLDFGPETIEDIAIVTGSGTDWIREAEAAGVDALVTGEGKQKAYHEAREAGISVFLAGHYATETFGVRRIAEPIEEWGLETTFLDHPTGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q3INS9</accession>
    <name>Q3INS9_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">succinate dehydrogenase</fullName>
            <ecNumber evidence="4">1.3.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">sdhA</name>
        <name evidence="15" type="ordered locus">NP_4264A</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3INS9"/>
    <dbReference type="STRING" id="348780.NP_4264A"/>
    <dbReference type="EnsemblBacteria" id="CAI50223">
        <property type="protein sequence ID" value="CAI50223"/>
        <property type="gene ID" value="NP_4264A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_4264A"/>
    <dbReference type="eggNOG" id="arCOG00571">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014312_6_2_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.700.10:FF:000001">
        <property type="entry name" value="Mitochondrial succinate dehydrogenase flavoprotein subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.100:FF:000001">
        <property type="entry name" value="Succinate dehydrogenase flavoprotein subunit (SdhA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.100">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase flavoprotein-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-dep_OxRdtase_2_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003952">
        <property type="entry name" value="FRD_SDH_FAD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037099">
        <property type="entry name" value="Fum_R/Succ_DH_flav-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015939">
        <property type="entry name" value="Fum_Rdtase/Succ_DH_flav-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030664">
        <property type="entry name" value="SdhA/FrdA/AprA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE 2 FLAVOPROTEIN SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632:SF51">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02910">
        <property type="entry name" value="Succ_DH_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000171">
        <property type="entry name" value="SDHA_APRA_LASPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46977">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00504">
        <property type="entry name" value="FRD_SDH_FAD_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FAD-dependent oxidoreductase 2 FAD-binding" evidence="13">
        <location>
            <begin position="7"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="Fumarate reductase/succinate dehydrogenase flavoprotein-like C-terminal" evidence="14">
        <location>
            <begin position="482"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="406"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000171-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAI50223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="611" mass="67105" checksum="899361887955FD7F" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IQA0</accession>
    <name>RS3A_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein eS1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">30S ribosomal protein S3Ae</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Ribosomal protein S1e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps3ae</name>
        <name type="ordered locus">NP_3210A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323318.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IQA0"/>
    <dbReference type="SMR" id="Q3IQA0"/>
    <dbReference type="STRING" id="348780.NP_3210A"/>
    <dbReference type="EnsemblBacteria" id="CAI49696">
        <property type="protein sequence ID" value="CAI49696"/>
        <property type="gene ID" value="NP_3210A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703181"/>
    <dbReference type="KEGG" id="nph:NP_3210A"/>
    <dbReference type="eggNOG" id="arCOG04186">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062507_1_0_2"/>
    <dbReference type="OrthoDB" id="30639at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00359">
        <property type="entry name" value="Ribosomal_eS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001593">
        <property type="entry name" value="Ribosomal_eS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030838">
        <property type="entry name" value="Ribosomal_eS1_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003142">
        <property type="entry name" value="PRK04057.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11830">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01015">
        <property type="entry name" value="Ribosomal_S3Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01397">
        <property type="entry name" value="Ribosomal_S3Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_1000005197" description="Small ribosomal subunit protein eS1">
        <location>
            <begin position="1"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="206" mass="23316" checksum="50C77E9AC47CC3C5" modified="2005-11-08" version="1">MSERSVSKQKQEKRWYTVMAPEIFDRAELGETPADEPEQVYDRTVQTTLGELQNDPSENNTKLTFQISDVGSDTAYTDFVQHELTRDYLRSLTRRGTSKVDAFVTVLTTDDYRVQVQPVAYTTKSADRSQEQAIRSTMVDLVEESAADNTFADLIDSIVEGRLSSAIYNEAKTIYPLRRVEIQKTRLQASPEEVAAEEEASVDVDD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ITA0</accession>
    <name>UVRC_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UvrABC system protein C</fullName>
            <shortName evidence="1">Protein UvrC</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Excinuclease ABC subunit C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">uvrC</name>
        <name type="ordered locus">NP_1086A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrC both incises the 5' and 3' sides of the lesion. The N-terminal half is responsible for the 3' incision and the C-terminal half is responsible for the 5' incision.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with UvrB in an incision complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UvrC family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322270.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ITA0"/>
    <dbReference type="SMR" id="Q3ITA0"/>
    <dbReference type="STRING" id="348780.NP_1086A"/>
    <dbReference type="EnsemblBacteria" id="CAI48634">
        <property type="protein sequence ID" value="CAI48634"/>
        <property type="gene ID" value="NP_1086A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703459"/>
    <dbReference type="KEGG" id="nph:NP_1086A"/>
    <dbReference type="eggNOG" id="arCOG00873">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG04753">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014841_3_1_2"/>
    <dbReference type="OrthoDB" id="121419at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009380">
        <property type="term" value="C:excinuclease repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009381">
        <property type="term" value="F:excinuclease ABC activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd10434">
        <property type="entry name" value="GIY-YIG_UvrC_Cho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1440.10:FF:000001">
        <property type="entry name" value="UvrABC system protein C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1440.10">
        <property type="entry name" value="GIY-YIG endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.10">
        <property type="entry name" value="UVR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.340">
        <property type="entry name" value="UvrC, RNAse H endonuclease domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00203">
        <property type="entry name" value="UvrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000305">
        <property type="entry name" value="GIY-YIG_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035901">
        <property type="entry name" value="GIY-YIG_endonuc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047296">
        <property type="entry name" value="GIY-YIG_UvrC_Cho"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001943">
        <property type="entry name" value="UVR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036876">
        <property type="entry name" value="UVR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050066">
        <property type="entry name" value="UvrABC_protein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004791">
        <property type="entry name" value="UvrC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001162">
        <property type="entry name" value="UvrC_RNase_H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038476">
        <property type="entry name" value="UvrC_RNase_H_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011262">
        <property type="entry name" value="PRK14668.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00194">
        <property type="entry name" value="uvrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30562:SF1">
        <property type="entry name" value="UVRABC SYSTEM PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30562">
        <property type="entry name" value="UVRC/OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01541">
        <property type="entry name" value="GIY-YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14520">
        <property type="entry name" value="HHH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22920">
        <property type="entry name" value="UvrC_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08459">
        <property type="entry name" value="UvrC_RNaseH_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00465">
        <property type="entry name" value="GIYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46600">
        <property type="entry name" value="C-terminal UvrC-binding domain of UvrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82771">
        <property type="entry name" value="GIY-YIG endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50164">
        <property type="entry name" value="GIY_YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50165">
        <property type="entry name" value="UVRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0228">DNA excision</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0267">Excision nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0742">SOS response</keyword>
    <feature type="chain" id="PRO_0000227498" description="UvrABC system protein C">
        <location>
            <begin position="1"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="domain" description="GIY-YIG" evidence="1">
        <location>
            <begin position="15"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="UVR" evidence="1">
        <location>
            <begin position="198"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00203"/>
        </source>
    </evidence>
    <sequence length="575" mass="63154" checksum="0C2FE3F7F01891CE" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ITD8</accession>
    <name>Q3ITD8_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA 2',3'-cyclic phosphodiesterase</fullName>
            <shortName evidence="2">RNA 2',3'-CPDase</shortName>
            <ecNumber evidence="2">3.1.4.58</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">thpR</name>
        <name evidence="3" type="ordered locus">NP_1010A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Hydrolyzes RNA 2',3'-cyclic phosphodiester to an RNA 2'-phosphomonoester.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + H2O = a 3'-end 2'-phospho-ribonucleotide-RNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11828"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:17353"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:173113"/>
            <dbReference type="EC" id="3.1.4.58"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 2H phosphoesterase superfamily. ThpR family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.58" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322231.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ITD8"/>
    <dbReference type="STRING" id="348780.NP_1010A"/>
    <dbReference type="EnsemblBacteria" id="CAI48596">
        <property type="protein sequence ID" value="CAI48596"/>
        <property type="gene ID" value="NP_1010A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701031"/>
    <dbReference type="KEGG" id="nph:NP_1010A"/>
    <dbReference type="eggNOG" id="arCOG01736">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081251_3_4_2"/>
    <dbReference type="OrthoDB" id="44091at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004113">
        <property type="term" value="F:2',3'-cyclic-nucleotide 3'-phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008664">
        <property type="term" value="F:RNA 2',3'-cyclic 3'-phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1140.10">
        <property type="entry name" value="Cyclic phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01940">
        <property type="entry name" value="RNA_CPDase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009097">
        <property type="entry name" value="Cyclic_Pdiesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004175">
        <property type="entry name" value="RNA_CPDase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02258">
        <property type="entry name" value="2_5_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35561">
        <property type="entry name" value="RNA 2',3'-CYCLIC PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35561:SF1">
        <property type="entry name" value="RNA 2',3'-CYCLIC PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13563">
        <property type="entry name" value="2_5_RNA_ligase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55144">
        <property type="entry name" value="LigT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="short sequence motif" description="HXTX 1" evidence="2">
        <location>
            <begin position="38"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="HXTX 2" evidence="2">
        <location>
            <begin position="126"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="38"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="182" mass="19676" checksum="20EB519EEC3B3D9D" modified="2005-11-08" version="1">MRLFVSATVAPLSDAIADIQAPLRGLDGLRPTDPQQAHITMKFLGAGDHDTDALAAAIETAVADADTGPFEVSLEGVGVFPSLEYISVVWLGVGGGSRELTRLHEQLEAETTALGYDAESHEFTPHVTLARMDNAASKAEVQEFVQTADPDAGRVRLEELRLTESTLTPDGPEYETRRRFEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-12-04" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ITF7</accession>
    <name>MTAD_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">5-methylthioadenosine/S-adenosylhomocysteine deaminase</fullName>
            <shortName evidence="1">MTA/SAH deaminase</shortName>
            <ecNumber evidence="1">3.5.4.28</ecNumber>
            <ecNumber evidence="1">3.5.4.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mtaD</name>
        <name type="ordered locus">NP_0972A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the deamination of 5-methylthioadenosine and S-adenosyl-L-homocysteine into 5-methylthioinosine and S-inosyl-L-homocysteine, respectively. Is also able to deaminate adenosine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-adenosyl-L-homocysteine + H2O + H(+) = S-inosyl-L-homocysteine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:20716"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:57985"/>
            <dbReference type="EC" id="3.5.4.28"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-methyl-5'-thioadenosine + H2O + H(+) = S-methyl-5'-thioinosine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:25025"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:48595"/>
            <dbReference type="EC" id="3.5.4.31"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. MTA/SAH deaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.28" evidence="1"/>
    <dbReference type="EC" id="3.5.4.31" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322212.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ITF7"/>
    <dbReference type="SMR" id="Q3ITF7"/>
    <dbReference type="STRING" id="348780.NP_0972A"/>
    <dbReference type="EnsemblBacteria" id="CAI48577">
        <property type="protein sequence ID" value="CAI48577"/>
        <property type="gene ID" value="NP_0972A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702786"/>
    <dbReference type="KEGG" id="nph:NP_0972A"/>
    <dbReference type="eggNOG" id="arCOG00695">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012358_2_1_2"/>
    <dbReference type="OrthoDB" id="372084at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090614">
        <property type="term" value="F:5'-methylthioadenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050270">
        <property type="term" value="F:S-adenosylhomocysteine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01298">
        <property type="entry name" value="ATZ_TRZ_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000014">
        <property type="entry name" value="5-methylthioadenosine/S-adenosylhomocysteine deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01281">
        <property type="entry name" value="MTA_SAH_deamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023512">
        <property type="entry name" value="Deaminase_MtaD/DadD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050287">
        <property type="entry name" value="MTA/SAH_deaminase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43794:SF11">
        <property type="entry name" value="AMIDOHYDROLASE-RELATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43794">
        <property type="entry name" value="AMINOHYDROLASE SSNA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000312484" description="5-methylthioadenosine/S-adenosylhomocysteine deaminase">
        <location>
            <begin position="1"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01281"/>
        </source>
    </evidence>
    <sequence length="431" mass="45106" checksum="C0F5F31585720A0D" modified="2005-11-08" version="1">MSTLQITGGQVLTPELTVRTADVLADSETGEILAVGDTAAGDRTLDAEGCLVVPGLVNTHCHAAMTLLRGYADDKPLDRWLQEDIWPVEAELTPEDIRAGTRLGLVELLKNGVTAVGDMYFEVPEVAAAVEEAGIRARLGHGIVTVGKDEADARADFEEGLAVARELDGAADGRVRTALMPHSLTTADPDLIAEFVPRARDAGVPIHYHANETTDEVDPIVDERGVRPLEFADELGLLDEGDFIAHGVHVDETEIELLAERGVGVAHCPASNMKLASGIAPVQEFLDAGVTVGIGTDGPASNNDLDVVDEMRDAAMVGKLGADDAAAVAAPDIVNAATAGGAETLGFDAGRVEAGALADLAIVDLDAPHLTPSHDLVSHLAYAVRGSDVRHTVVGGEVIVEDREVRTLDAGAVKREAERHAAELVSRAEGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IU09</accession>
    <name>Q3IU09_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Enamine/imine deaminase</fullName>
            <ecNumber evidence="2">3.5.99.10</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ridA</name>
        <name evidence="2" type="ordered locus">NP_0566A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RutC family.</text>
    </comment>
    <dbReference type="EC" id="3.5.99.10" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322010.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IU09"/>
    <dbReference type="STRING" id="348780.NP_0566A"/>
    <dbReference type="EnsemblBacteria" id="CAI48374">
        <property type="protein sequence ID" value="CAI48374"/>
        <property type="gene ID" value="NP_0566A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703063"/>
    <dbReference type="KEGG" id="nph:NP_0566A"/>
    <dbReference type="eggNOG" id="arCOG01630">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100715_7_1_2"/>
    <dbReference type="OrthoDB" id="211138at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120241">
        <property type="term" value="F:2-iminobutanoate/2-iminopropanoate deaminase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00448">
        <property type="entry name" value="YjgF_YER057c_UK114_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.40:FF:000001">
        <property type="entry name" value="L-PSP family endoribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="entry name" value="RutC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006056">
        <property type="entry name" value="RidA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019897">
        <property type="entry name" value="RidA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006175">
        <property type="entry name" value="YjgF/YER057c/UK114"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00004">
        <property type="entry name" value="Rid family detoxifying hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803">
        <property type="entry name" value="2-IMINOBUTANOATE/2-IMINOPROPANOATE DEAMINASE RIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803:SF58">
        <property type="entry name" value="PROTEIN HMF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01042">
        <property type="entry name" value="Ribonuc_L-PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="YjgF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01094">
        <property type="entry name" value="UPF0076"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="127" mass="13140" checksum="991E5B1643960553" modified="2005-11-08" version="1">MSKEIVSTDEAPAAAGAYSQATTDGNLIFTAGQIAMTPDGDSLADEPIEVQTERALKNLEAVLEAAGAGLDDVLKTTVYLADIDDFEAMNGTYADFFDGEPPARSAVEAGDLPLGMGVEIEAVATKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ET82</accession>
    <name>A0A1U7ET82_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">ABC-type transport system ATP-binding/permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">NP_0006A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321733.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ET82"/>
    <dbReference type="STRING" id="348780.NP_0006A"/>
    <dbReference type="EnsemblBacteria" id="CAI48094">
        <property type="protein sequence ID" value="CAI48094"/>
        <property type="gene ID" value="NP_0006A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700737"/>
    <dbReference type="KEGG" id="nph:NP_0006A"/>
    <dbReference type="eggNOG" id="arCOG02841">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_3_2"/>
    <dbReference type="OrthoDB" id="121502at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18565">
        <property type="entry name" value="ABC_6TM_exporter_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000287">
        <property type="entry name" value="Multidrug ABC transporter ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221:SF654">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B MEMBER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="173"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="284"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="314"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="40"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="379"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI48094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="641" mass="70140" checksum="B3FB3BECE458265B" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ET96</accession>
    <name>A0A1U7ET96_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transport protein (Probable substrate cationic amino acids)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">cat1</name>
        <name evidence="7" type="synonym">tp01b</name>
        <name evidence="7" type="ordered locus">NP_0040A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321750.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ET96"/>
    <dbReference type="STRING" id="348780.NP_0040A"/>
    <dbReference type="EnsemblBacteria" id="CAI48111">
        <property type="protein sequence ID" value="CAI48111"/>
        <property type="gene ID" value="NP_0040A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703279"/>
    <dbReference type="KEGG" id="nph:NP_0040A"/>
    <dbReference type="eggNOG" id="arCOG03649">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_15_12_2"/>
    <dbReference type="OrthoDB" id="43026at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
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        <property type="entry name" value="APC_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770">
        <property type="entry name" value="AMINO ACID TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="119"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="233"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="291"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="349"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="377"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="419"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="445"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="491" mass="51414" checksum="C6FAE9427818F7C5" modified="2017-05-10" version="1">MSADDFKLINEQVGLWGAVALLVGTAVGMSIFIVPTQMAAVAGPSITMAILVSILPMILGVLLLLQLGGAIPVAGGIYVYGSRLVGPYWGMIGVAIPVLAVWSYILFAALGFAQYLDGLAATVAGVSVSTTLTVWGIVGLFLVLNYVGLRIVAAVQIGMVLFLLAGLVTFVVGMGPAVDTGNYTPMFPDDLFAGGLAPFLFAVVLLYIPFQGFGMIIEIGEELENPVKNIPRVLAIGMGLVAVVSIAVIFVLVGAVPWEQTIDPETVPEGGSVTEGEPVEGGLAAVAEGTIPAAGILFISLAALVAGATTINTLFTSYSRTVMRAARDEVVPGGLAKVHGRFGTPNRAVVLLGIPPLAAAPFAGTLDAVVGPDVLDWLVVVVVTGIFVAFMVGGVALWNLPKVFPQRYEYSIYKLPLPVLRVVAVGNVVVSVVFTALVATTAPTAFGLVLAWLIIVSAAYKYRIRAYERRGEDLRSRMALLHKHEQVGNDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETB2</accession>
    <name>A0A1U7ETB2_NATPD</name>
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        <submittedName>
            <fullName evidence="5">Lrp/AsnC family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_0078A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="RefSeq" id="WP_011321769.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
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    <dbReference type="STRING" id="348780.NP_0078A"/>
    <dbReference type="EnsemblBacteria" id="CAI48130">
        <property type="protein sequence ID" value="CAI48130"/>
        <property type="gene ID" value="NP_0078A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700754"/>
    <dbReference type="KEGG" id="nph:NP_0078A"/>
    <dbReference type="eggNOG" id="arCOG01580">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_5_4_2"/>
    <dbReference type="OrthoDB" id="6995at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043200">
        <property type="term" value="P:response to amino acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="HTH_ARSR"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
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        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
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        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
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        <property type="entry name" value="HTH_trans_regulator_Lrp"/>
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    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041396">
        <property type="entry name" value="TranRegLrp_Halo"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LEUCINE-RESPONSIVE REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154:SF34">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR AZLB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="3"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI48130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="150" mass="16745" checksum="CB8FDB4809A7A8D9" modified="2017-05-10" version="1">MTYENLDKKLVNALLEDGRASLRSLGEDLDVSVTTVSNHISDLEDDGIIEGYTPVVNYGKLGYDVTAIIQMKVEGSSLPDITDDLREHKHMVSVYEVTGDHDIIAVGKFTDTDHMNEQIKELLVDPEIKESNTSVVLNTVAENEQFELDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETC0</accession>
    <name>A0A1U7ETC0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CARDB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0098A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321779.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETC0"/>
    <dbReference type="STRING" id="348780.NP_0098A"/>
    <dbReference type="EnsemblBacteria" id="CAI48140">
        <property type="protein sequence ID" value="CAI48140"/>
        <property type="gene ID" value="NP_0098A"/>
    </dbReference>
    <dbReference type="GeneID" id="69102733"/>
    <dbReference type="KEGG" id="nph:NP_0098A"/>
    <dbReference type="eggNOG" id="arCOG04400">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040511_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35902:SF6">
        <property type="entry name" value="CONSERVED WITHIN P. AEROPHILUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35902">
        <property type="entry name" value="S-LAYER DOMAIN-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="396"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="426" mass="44273" checksum="B9FE214E2110DA10" modified="2017-05-10" version="1">MAHRRTLLFAVVLVALGAIAVPVVADVPDARLVVSDVEVTPEAPEPGNTTSVEFTVENSAGSATGVDIEAIELRERARVGETHAAAGNVGSLSAGDDVTLSLSTAFEEAGVHDLELAVTAVDEAGAEVTVTRPVTIVVGGVDAAGITDDVQIDVQEVDPAELDDEEDDLDVDLGADGILGDGDDEDEESRTSLIRVEVTNFGTATAREVAVTPAVEDGPELAEPPARLALPDVQPGDAESVFFNAAAFDEPTTLSFEAGYTLGTERETSETTFEYRPNRGEVILTDVDMTSEDGTVTVTGNAANPGLGEINGAVVAVEETETVSPTYPAREYFVGTVPESEFVRFDVTADVDHDNATTVPITVTYLADGEPYERTVELPYEPRTADRGDSGGGDGVPLSVAAAVAGSALVAGSVAVGWRRRSRAGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETF7</accession>
    <name>A0A1U7ETF7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SWIM zinc finger domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0196A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48189.3"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETF7"/>
    <dbReference type="STRING" id="348780.NP_0196A"/>
    <dbReference type="EnsemblBacteria" id="CAI48189">
        <property type="protein sequence ID" value="CAI48189"/>
        <property type="gene ID" value="NP_0196A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_0196A"/>
    <dbReference type="eggNOG" id="arCOG01121">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153623_0_0_2"/>
    <dbReference type="OrthoDB" id="31559at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="2">
        <location>
            <begin position="51"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48189.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="115" mass="13286" checksum="D12B158A0FADAFC8" modified="2017-05-10" version="1">MNAVETWERTLEADGELTSDAVEQIIDTHDDRGVRAIEAVSEERVKQYRDFTVVVGHSEEYVIEDDGCSCEDSRYNLDPEDPTALCWHVIAAKIARRIDAVDHHDMWYSEVREFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETN4</accession>
    <name>A0A1U7ETN4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable secreted glycoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0362A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48272.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETN4"/>
    <dbReference type="STRING" id="348780.NP_0362A"/>
    <dbReference type="EnsemblBacteria" id="CAI48272">
        <property type="protein sequence ID" value="CAI48272"/>
        <property type="gene ID" value="NP_0362A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_0362A"/>
    <dbReference type="eggNOG" id="arCOG02087">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_266656_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1120">
        <property type="entry name" value="Carboxypeptidase-like, regulatory domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008969">
        <property type="entry name" value="CarboxyPept-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008965">
        <property type="entry name" value="CBM2/CBM3_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13620">
        <property type="entry name" value="CarboxypepD_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49384">
        <property type="entry name" value="Carbohydrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49464">
        <property type="entry name" value="Carboxypeptidase regulatory domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="752"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="981"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="752"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="981"/>
            <end position="998"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48272.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="1044" mass="109406" checksum="9F8BB4B3A68735C0" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETT6</accession>
    <name>A0A1U7ETT6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Thioesterase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0518A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 4-hydroxybenzoyl-CoA thioesterase family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETT6"/>
    <dbReference type="STRING" id="348780.NP_0518A"/>
    <dbReference type="EnsemblBacteria" id="CAI48350">
        <property type="protein sequence ID" value="CAI48350"/>
        <property type="gene ID" value="NP_0518A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_0518A"/>
    <dbReference type="eggNOG" id="arCOG01137">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101141_5_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00586">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050563">
        <property type="entry name" value="4-hydroxybenzoyl-CoA_TE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006684">
        <property type="entry name" value="YbgC/YbaW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31793">
        <property type="entry name" value="4-HYDROXYBENZOYL-COA THIOESTERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31793:SF27">
        <property type="entry name" value="NOVEL THIOESTERASE SUPERFAMILY DOMAIN AND SAPOSIN A-TYPE DOMAIN CONTAINING PROTEIN (0610012H03RIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13279">
        <property type="entry name" value="4HBT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003230">
        <property type="entry name" value="YbgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="148" mass="17071" checksum="FAE55C6E1C3A2888" modified="2017-05-10" version="1">MFPARPGRAMHDVYENTVRFAETDKQGIVFYGNYATYQDETFTAFMEVVGYPYEELKERGWDVHVVNLELSYRKPAEFREQLTNAMRASAIRDTSIEFDYECRNADGDLLVEGTVTHVTVDESGEPTRVPESFRDAIVEFQEEPPEPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETY6</accession>
    <name>A0A1U7ETY6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0602A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322028.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETY6"/>
    <dbReference type="STRING" id="348780.NP_0602A"/>
    <dbReference type="EnsemblBacteria" id="CAI48392">
        <property type="protein sequence ID" value="CAI48392"/>
        <property type="gene ID" value="NP_0602A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700903"/>
    <dbReference type="KEGG" id="nph:NP_0602A"/>
    <dbReference type="eggNOG" id="arCOG04770">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096707_0_0_2"/>
    <dbReference type="OrthoDB" id="205088at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="231" mass="23632" checksum="83C07C6D6629E099" modified="2017-05-10" version="1">MDVLGDIIGRDRRTDAPALVAPAVGRDYDYRRFCTTAWKTGNFLRHLGVRGGAGVAVADDPLPEPVLTLYGAALLGGVVSFGPPSELDSPRAIVAPAADIDAEAVEPGTKPVAYGDDPGDPTVAYFERDVWSENPTEPPDHIAGTDPLLRAGEERYSHGDVLTAARTAIDDHGIDDGTTVAVGGSFRSPGVVAAGLVAPIVAGGTVAVGPAPEGDVQVGGEGSDVETDALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU05</accession>
    <name>A0A1U7EU05_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">FMN-binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0640A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322047.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU05"/>
    <dbReference type="EnsemblBacteria" id="CAI48411">
        <property type="protein sequence ID" value="CAI48411"/>
        <property type="gene ID" value="NP_0640A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700916"/>
    <dbReference type="KEGG" id="nph:NP_0640A"/>
    <dbReference type="eggNOG" id="arCOG00516">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1718154_0_0_2"/>
    <dbReference type="OrthoDB" id="194652at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011576">
        <property type="entry name" value="Pyridox_Oxase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01243">
        <property type="entry name" value="PNPOx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyridoxamine 5'-phosphate oxidase N-terminal" evidence="1">
        <location>
            <begin position="11"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="153" mass="16920" checksum="50E29E6A39EE14AD" modified="2017-05-10" version="1">MDVVENTLGASIDAFLSRPLFGHLATHHPDGPRESPVWYLWEDDTAWVLGDSATDTFPARIEQEPACALGVVDFDAERGLVQHVGIRGRGSVEAFNRDRAERLLARYLGADRSGWDRRFADYIADPTETALLVRVEPETVVARDQSYAPAPVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU80</accession>
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    </protein>
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        <name evidence="8" type="primary">mrpD3</name>
        <name evidence="8" type="synonym">tp49i</name>
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        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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    </dbReference>
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    </dbReference>
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    <dbReference type="EnsemblBacteria" id="CAI48504">
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        <property type="gene ID" value="NP_0826A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703335"/>
    <dbReference type="KEGG" id="nph:NP_0826A"/>
    <dbReference type="eggNOG" id="arCOG01537">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="101192at2157"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050586">
        <property type="entry name" value="CPA3_Na-H_Antiporter_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003918">
        <property type="entry name" value="NADH_UbQ_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42703:SF1">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER SUBUNIT D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42703">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01437">
        <property type="entry name" value="NUOXDRDTASE4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="208"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="246"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="282"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="307"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="338"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="371"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="412"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="453"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:quinone oxidoreductase/Mrp antiporter transmembrane" evidence="7">
        <location>
            <begin position="130"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI48504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="491" mass="51614" checksum="4DBEFD54F66E2D18" modified="2017-05-10" version="1">MDAYSWLLAVIILLPLISASVPIAASVRWKNVGWAIAAVVVSATFAMSVALAWMVPRAEDRLLAHQLGGVEVPIGIELFADEFSVIVLLLVLAVCLGVLVFTRIGGPRGNAFYSGFLLLVGGMMGILLTGDLFNMYVFLEIMAISSYALVSASKYRWSTYAAFKYLLVGTVGAGFYLLGVALVLASTGTLTMRLLPELLAAVGYTDPVVVAAFILMAVGLAIKIALFPVHTWLADAHASAPDGISAVISALVPAVAVYAFARVLFTVYTLDFIAANPVIGNLLLVAALFSLFAGSFFALLQDHIKLVLAYSTVSQFGLIFVGIAIANETAVFGAILQIFGHGVVKASLFVLAGMFALRFGGIRTLKEYSGVAERAPIMAGAFAVLGIAMIGLPPTVGFAGKWYIALGAFEAGLWQVSLLVLGSTLLSAGYILPFINRIYFHPFEGEDVDAAKVTTGMLIAIIIGAGIGLLLGFASAEIQAAVSTAVERLVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU84</accession>
    <name>A0A1U7EU84_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Mrp-type sodium/proton antiporter system subunit B1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">mrpB1</name>
        <name evidence="8" type="synonym">tp49d</name>
        <name evidence="8" type="ordered locus">NP_0836A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322145.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU84"/>
    <dbReference type="STRING" id="348780.NP_0836A"/>
    <dbReference type="EnsemblBacteria" id="CAI48509">
        <property type="protein sequence ID" value="CAI48509"/>
        <property type="gene ID" value="NP_0836A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703330"/>
    <dbReference type="KEGG" id="nph:NP_0836A"/>
    <dbReference type="eggNOG" id="arCOG03077">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102546_0_0_2"/>
    <dbReference type="OrthoDB" id="99605at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
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    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1200">
        <property type="entry name" value="NADH-ubiquinone/plastoquinone oxidoreductase chain 6, subunit NuoJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025383">
        <property type="entry name" value="MrpA_C/MbhD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042106">
        <property type="entry name" value="Nuo/plastoQ_OxRdtase_6_NuoJ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009159">
        <property type="entry name" value="PRK12504.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13244">
        <property type="entry name" value="MbhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="117"/>
        </location>
    </feature>
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        <location>
            <begin position="172"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="MrpA C-terminal/MbhD" evidence="7">
        <location>
            <begin position="12"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI48509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="195" mass="20302" checksum="9FAD81318B838FA4" modified="2017-05-10" version="1">MMWLGLEFALILFVVAAAVFVAFARDIVGGIVTYAAFTLGIAVIWVLLAAPDVALIEAAVGAGVTSVLFLITVMKTTGMTGEDEAETGDSDGRFRPVNIPAVLMVGGLAIPLGYVILSLDPVGAEGAPAVTETYADGSLTPYGHYIQETLEETGFPNAVVAVLVVYRGLDTLGELIVAFAAAVSILVVLEREDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU91</accession>
    <name>A0A1U7EU91_NATPD</name>
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        <submittedName>
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        </submittedName>
    </protein>
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        <name evidence="4" type="ordered locus">NP_0858A</name>
    </gene>
    <organism evidence="4 5">
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        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
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            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
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        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="STRING" id="348780.NP_0858A"/>
    <dbReference type="ESTHER" id="natpd-q3itl3">
        <property type="family name" value="Hormone-sensitive_lipase_like"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI48520">
        <property type="protein sequence ID" value="CAI48520"/>
        <property type="gene ID" value="NP_0858A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700979"/>
    <dbReference type="KEGG" id="nph:NP_0858A"/>
    <dbReference type="eggNOG" id="arCOG02638">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012494_6_4_2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002168">
        <property type="entry name" value="Lipase_GDXG_HIS_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF8">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-3 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01173">
        <property type="entry name" value="LIPASE_GDXG_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="3">
        <location>
            <begin position="76"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="313" mass="33227" checksum="5F7C98FAA96495E4" modified="2017-05-10" version="1">MADAPDDQLQPILDLLADAPALEHLGVDGARQQFEALGSFAPTYDVHDEVDITIPGPDGDLDARVFRAAAEARPTLLYFHGGGFVVGSIDTHANICQRLAEQSGWHVVSVAYRLAPEHPFPAALEDACAAAEWVADNPDAVGSDGTLAVGGDSAGGNLAAGVSLLARDTREDSGSDLPDIAHQVLYYPVCGSPLAHYESRESSETGYFLEQSTMEWFHDQYVQSPVHNRNEYLAPLLVDDYTGLPSATVVTAGLDPLRDEGCAYAEALDDDGVDVFHAHYDSMVHGFVSFLGMVAAADDAVRATVSGLDAAID</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUB5</accession>
    <name>A0A1U7EUB5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">Nicotinamide-nucleotide adenylyltransferase</fullName>
            <ecNumber evidence="6 7">2.7.7.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">NAD(+) diphosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">NAD(+) pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">NMN adenylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">nadM1</name>
        <name evidence="9" type="ordered locus">NP_0908A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>beta-nicotinamide D-ribonucleotide + ATP + H(+) = diphosphate + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:21360"/>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="EC" id="2.7.7.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from nicotinamide D-ribonucleotide: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the archaeal NMN adenylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.1" evidence="6 7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322181.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUB5"/>
    <dbReference type="STRING" id="348780.NP_0908A"/>
    <dbReference type="EnsemblBacteria" id="CAI48545">
        <property type="protein sequence ID" value="CAI48545"/>
        <property type="gene ID" value="NP_0908A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702966"/>
    <dbReference type="KEGG" id="nph:NP_0908A"/>
    <dbReference type="eggNOG" id="arCOG00972">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108783_0_0_2"/>
    <dbReference type="OrthoDB" id="264480at2157"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00600"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000309">
        <property type="term" value="F:nicotinamide-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02166">
        <property type="entry name" value="NMNAT_Archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00243">
        <property type="entry name" value="NMN_adenylyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006418">
        <property type="entry name" value="NMN_Atrans_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01527">
        <property type="entry name" value="arch_NMN_Atrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002243">
        <property type="entry name" value="PRK01153.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21342:SF0">
        <property type="entry name" value="BIFUNCTIONAL NMN ADENYLYLTRANSFERASE_NUDIX HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21342">
        <property type="entry name" value="PHOSPHOPANTETHEINE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 6 9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="2 6" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="8">
        <location>
            <begin position="5"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="NCBIfam" id="TIGR01527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI48545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="173" mass="19560" checksum="2D7DB282E3C2E79B" modified="2017-05-10" version="1">MTRGFYIGRFQPFHEGHHAMVDRIREEVDELVVGIGSADQSHTVRNPFTAGERIMMITKALDKFETTTYAVPIEDLNRNSVWVSHVQSMSPRFDVAYSNNPLVVRLFKEAGIEVRQSPMYQREEFSGTEVRERMATGGDWEALVPDAVVSVIDEIDGVDRIRQITATDANGDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUC1</accession>
    <name>A0A1U7EUC1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Lrp/AsnC family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">NP_0924A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322189.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUC1"/>
    <dbReference type="STRING" id="348780.NP_0924A"/>
    <dbReference type="EnsemblBacteria" id="CAI48553">
        <property type="protein sequence ID" value="CAI48553"/>
        <property type="gene ID" value="NP_0924A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700999"/>
    <dbReference type="KEGG" id="nph:NP_0924A"/>
    <dbReference type="eggNOG" id="arCOG01580">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_5_4_2"/>
    <dbReference type="OrthoDB" id="6995at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043200">
        <property type="term" value="P:response to amino acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154">
        <property type="entry name" value="LEUCINE-RESPONSIVE REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154:SF34">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR AZLB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="124"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI48553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="148" mass="16214" checksum="ABD602A834EA4C56" modified="2017-05-10" version="1">MGERLDRQLVAALCRDARADITDIAAPTDAVATTVQKRLRALEDDGAIDGYAPRLNYERLGYETVIFQLSVPLGSVDDVTDRLRATEAFVTVYQTDGDRTVFAVGKFEDESAVAAQLHKLHDDPDIRDVDTSPVQSVHAEGDCPIPLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUD1</accession>
    <name>A0A1U7EUD1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protease HtpX homolog</fullName>
            <ecNumber evidence="11">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">htpX1</name>
        <name evidence="11" type="synonym">htpX</name>
        <name evidence="13" type="ordered locus">NP_0964A</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the peptidase M48B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="11"/>
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        <property type="protein sequence ID" value="CAI48573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
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    <dbReference type="STRING" id="348780.NP_0964A"/>
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        <property type="gene ID" value="NP_0964A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702834"/>
    <dbReference type="KEGG" id="nph:NP_0964A"/>
    <dbReference type="eggNOG" id="arCOG01331">
        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="OrthoDB" id="28389at2157"/>
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    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="M48B_HtpX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.10">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00188">
        <property type="entry name" value="Pept_M48_protease_HtpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050083">
        <property type="entry name" value="HtpX_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022919">
        <property type="entry name" value="Pept_M48_protease_HtpX"/>
    </dbReference>
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        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002669">
        <property type="entry name" value="PRK02391.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43221">
        <property type="entry name" value="PROTEASE HTPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43221:SF2">
        <property type="entry name" value="PROTEASE HTPX HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 11 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3 11 13" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7 11" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M48" evidence="12">
        <location>
            <begin position="74"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
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            <note>catalytic</note>
        </ligand>
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        <location>
            <position position="142"/>
        </location>
        <ligand>
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            <note>catalytic</note>
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        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
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            <note>catalytic</note>
        </ligand>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00009779"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="HAMAP-Rule" id="MF_00188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAI48573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="290" mass="32115" checksum="61C4E92BD3E3FC46" modified="2017-05-10" version="1">MQWETDWGLRARMGLTMFLLFALYLVFVGVLTLYFEGLLVPAILLGGFSIAQFFFSDKLALRSMGARTVDQEEYPELHAKVGRLAQQADLPKPTVAVADNQTPNAFATGRSPSNSAVCVTTGLLQTLDDEELEGVLAHELAHIKNRDVAVMTIASFLSTIAFLIVRWGWLFSGGRGGRNQAPLIVAILVSLLVWVLSFLLIRALSRYREYSADRGAAAITGKPSALATALMKISGRMDEVPQEDLREQAEMNAFFIIPISKGFIAKLASTHPPTETRIEKLRELEREMER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUE7</accession>
    <name>A0A1U7EUE7_NATPD</name>
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        <submittedName>
            <fullName evidence="2">DUF2237 family protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">NP_1000A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
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    <dbReference type="EnsemblBacteria" id="CAI48591">
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        <property type="gene ID" value="NP_1000A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701027"/>
    <dbReference type="KEGG" id="nph:NP_1000A"/>
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    <dbReference type="Gene3D" id="3.30.56.110">
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        <property type="entry name" value="SLR1628 PROTEIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR37466:SF1">
        <property type="entry name" value="SLR1628 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09996">
        <property type="entry name" value="DUF2237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI48591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="130" mass="14586" checksum="7F79D63B22EFFEED" modified="2017-05-10" version="1">MDETNVLGEPLEPCSTDPETGFERDGHCRCHPQDRGRHELCAMMTEEFLTFSADRGNDLMTPRPELEFPGLDPGDRWCLCVGRWVEALEAVRNQHIPETTVPPVILAATNESVLDTVPLETLQAHAYDEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUF3</accession>
    <name>A0A1U7EUF3_NATPD</name>
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        <submittedName>
            <fullName evidence="3">DUF2157 domain protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ordered locus">NP_0992A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
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            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
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            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
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            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48587.1"/>
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    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUF3"/>
    <dbReference type="STRING" id="348780.NP_0992A"/>
    <dbReference type="EnsemblBacteria" id="CAI48587">
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        <property type="gene ID" value="NP_0992A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701023"/>
    <dbReference type="KEGG" id="nph:NP_0992A"/>
    <dbReference type="eggNOG" id="arCOG14716">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_755706_0_0_2"/>
    <dbReference type="OrthoDB" id="137285at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018677">
        <property type="entry name" value="DUF2157"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09925">
        <property type="entry name" value="DUF2157"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="310"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="337"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2157" evidence="2">
        <location>
            <begin position="11"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="366" mass="37620" checksum="FE1786E3C803A6C9" modified="2017-05-10" version="1">MDPDRLADEVDQWVRDGIITESQAEAILQRYDTEATGRSRVVVALSVVGAALVFVGVALFFATRWAGLPTLLRGAVLVAAPSLAYAGGVVGYRRGTPRVGLALCLLGATLVGPSLFLLGDLFSVTFAEQWLLFAWSLAAVSTGHLLVSRVGVGLGLALVTALVAVLADPGDPLPPVAFFGIAVFGLASINDGRIARTYRLVGVVVTLAGLLGLTTLDGRFDRFDVGPTVFLFVTAAAALTSADWLLHIDATRDFEWATGAFLAVVAATALAVAAPGWIPSILAFAGIHAAMLAAVLATGYLGYRTGSRTLVDIATVAALVQTLSAVASTVVDSLSGAVALVAAGVVLLAAGVAIERGRRSVLSRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUG6</accession>
    <name>A0A1U7EUG6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Thermosome subunit 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ths1</name>
        <name evidence="7" type="ordered locus">NP_1008A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
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        <text evidence="1 5">Belongs to the TCP-1 chaperonin family.</text>
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    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:ATP hydrolysis activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Chaperone_TCP-1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="526"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="539"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="562" mass="58607" checksum="89281B21963FD3B3" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUL9</accession>
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        <recommendedName>
            <fullName evidence="4">YkgJ family cysteine cluster protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="2" type="ordered locus">NP_1134A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
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            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
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            <taxon>Natronomonas</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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    </dbReference>
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    <dbReference type="EnsemblBacteria" id="CAI48658">
        <property type="protein sequence ID" value="CAI48658"/>
        <property type="gene ID" value="NP_1134A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701061"/>
    <dbReference type="KEGG" id="nph:NP_1134A"/>
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    </dbReference>
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    <dbReference type="OrthoDB" id="36424at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="entry name" value="Puta_zinc/iron-chelating_dom"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35866:SF2">
        <property type="entry name" value="YKGJ FAMILY CYSTEINE CLUSTER PROTEIN"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF03692">
        <property type="entry name" value="CxxCxxCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="226" mass="24788" checksum="2D3E049F83E85D82" modified="2017-05-10" version="1">MESLEAALGRAESLSVDELADAIESIGFECTRCGGCCTAIDDEAGTEPHTATVFPDEVRQLQAAADERFETSYDWRDVARPMPYGLDEDGTGETFEWALQTDACGDCTFYEEADDGTGACTVHEDRPLVCQTYPFSLALGGTGEPMGEAVDREGLVRAHECEGLGRDIDRETAEELAETLKERSIRDLEEAIELRERYEPRPDADGAVVHDSEGSKRPDGTPLDRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUP4</accession>
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        <submittedName>
            <fullName evidence="2">NP_1176A family transcription regulator</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">NP_1176A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="STRING" id="348780.NP_1176A"/>
    <dbReference type="EnsemblBacteria" id="CAI48679">
        <property type="protein sequence ID" value="CAI48679"/>
        <property type="gene ID" value="NP_1176A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701075"/>
    <dbReference type="KEGG" id="nph:NP_1176A"/>
    <dbReference type="eggNOG" id="arCOG00394">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_176006_0_0_2"/>
    <dbReference type="OrthoDB" id="350804at2157"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
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        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="92" mass="10552" checksum="C00F2E30BA320BBC" modified="2017-05-10" version="1">MSTTTEERSRQSESPMSDPEFRETLRELPPSAKLVAKVLEGASPMSQGQLAEKSLLPDRTVRYALNRLEEEDLVDSRYSFNDARKQVYFLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUR2</accession>
    <name>A0A1U7EUR2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DICT domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1292A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322372.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUR2"/>
    <dbReference type="STRING" id="348780.NP_1292A"/>
    <dbReference type="EnsemblBacteria" id="CAI48737">
        <property type="protein sequence ID" value="CAI48737"/>
        <property type="gene ID" value="NP_1292A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701104"/>
    <dbReference type="KEGG" id="nph:NP_1292A"/>
    <dbReference type="eggNOG" id="arCOG02910">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069298_1_0_2"/>
    <dbReference type="OrthoDB" id="302327at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019278">
        <property type="entry name" value="DICT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016954">
        <property type="entry name" value="Uncharacterised_Vng0742h"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10069">
        <property type="entry name" value="DICT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF030471">
        <property type="entry name" value="STR_Vng0742h_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DICT" evidence="1">
        <location>
            <begin position="101"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="253" mass="27677" checksum="5E91CA031327F19C" modified="2017-05-10" version="1">MADSLCDFLKADDRSERKLAVVGDVEGPVQSLFEESFGGLDVSIESSGQSGGEEATVALVENGEVVATSPMGAIRNAILLVNSDLYTSGLSGIDKHEAPEVLTELDEQVVTLRGFPASVKEKLLLIVISRHIERRALEAGDGRLDVAFQRLSRLCDEYGTKKVYERLATTAVDVHAYGVPDGRPTDIPELTVHGNDSERYRRSWFVVFTPPPEGDTEPAALVAVETGENVWRSMWTYDAEQVAAIQQHIQEAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUR6</accession>
    <name>A0A1U7EUR6_NATPD</name>
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        <submittedName>
            <fullName evidence="2">UspA domain protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">NP_1290A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322371.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUR6"/>
    <dbReference type="STRING" id="348780.NP_1290A"/>
    <dbReference type="EnsemblBacteria" id="CAI48736">
        <property type="protein sequence ID" value="CAI48736"/>
        <property type="gene ID" value="NP_1290A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701103"/>
    <dbReference type="KEGG" id="nph:NP_1290A"/>
    <dbReference type="eggNOG" id="arCOG06388">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134823_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="1">
        <location>
            <begin position="2"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="134" mass="13887" checksum="AF8F84F329F6557F" modified="2017-05-10" version="1">MTFVVAFDGSADSEAALERAADAVDSDDRLLVVSVLPGDDRLADAYGFLANGTYDPEQVAADLETAVVAAAPAADFRTEQLGPYAGKQRIATVIAAVAREADADAVYLGSDNAGRVLDSLVDGTADYDVRLVCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUT4</accession>
    <name>A0A1U7EUT4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">DASS family transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">tp28</name>
        <name evidence="8" type="ordered locus">NP_1278A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322365.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUT4"/>
    <dbReference type="STRING" id="348780.NP_1278A"/>
    <dbReference type="EnsemblBacteria" id="CAI48730">
        <property type="protein sequence ID" value="CAI48730"/>
        <property type="gene ID" value="NP_1278A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703303"/>
    <dbReference type="KEGG" id="nph:NP_1278A"/>
    <dbReference type="eggNOG" id="arCOG00238">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005170_0_0_2"/>
    <dbReference type="OrthoDB" id="19068at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015141">
        <property type="term" value="F:succinate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01115">
        <property type="entry name" value="SLC13_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031312">
        <property type="entry name" value="Na/sul_symport_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001898">
        <property type="entry name" value="SLC13A/DASS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00785">
        <property type="entry name" value="dass"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283:SF82">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00939">
        <property type="entry name" value="Na_sulph_symp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01271">
        <property type="entry name" value="NA_SULFATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="97"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="145"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="211"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="264"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="309"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="366"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="396"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="434"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="465"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="497"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="526"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="554"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="57"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI48730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="595" mass="62249" checksum="8E16E98AD718287A" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUY4</accession>
    <name>A0A1U7EUY4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Molybdopterin molybdenumtransferase</fullName>
            <ecNumber evidence="5">2.10.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">moeA2</name>
        <name evidence="5" type="ordered locus">NP_1442A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">Gabara</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="2.10.1.1" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322447.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUY4"/>
    <dbReference type="STRING" id="348780.NP_1442A"/>
    <dbReference type="EnsemblBacteria" id="CAI48812">
        <property type="protein sequence ID" value="CAI48812"/>
        <property type="gene ID" value="NP_1442A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702933"/>
    <dbReference type="KEGG" id="nph:NP_1442A"/>
    <dbReference type="eggNOG" id="arCOG00217">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010186_3_0_2"/>
    <dbReference type="OrthoDB" id="31371at2157"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061599">
        <property type="term" value="F:molybdopterin molybdotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00887">
        <property type="entry name" value="MoeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.340.10">
        <property type="entry name" value="MoeA, C-terminal, domain IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.10">
        <property type="entry name" value="Molybdopterin biosynthesis moea protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.190.11">
        <property type="entry name" value="Molybdopterin biosynthesis moea protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008284">
        <property type="entry name" value="MoCF_biosynth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038987">
        <property type="entry name" value="MoeA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005111">
        <property type="entry name" value="MoeA_C_domain_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036688">
        <property type="entry name" value="MoeA_C_domain_IV_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005110">
        <property type="entry name" value="MoeA_linker/N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036135">
        <property type="entry name" value="MoeA_linker/N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024370">
        <property type="entry name" value="PBP_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045515">
        <property type="entry name" value="Glp_gephyrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011068">
        <property type="entry name" value="PRK14498.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192:SF5">
        <property type="entry name" value="GEPHYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192">
        <property type="entry name" value="MOLYBDOPTERIN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03454">
        <property type="entry name" value="MoeA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03453">
        <property type="entry name" value="MoeA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12727">
        <property type="entry name" value="PBP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63867">
        <property type="entry name" value="MoeA C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63882">
        <property type="entry name" value="MoeA N-terminal region -like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01079">
        <property type="entry name" value="MOCF_BIOSYNTHESIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MoaB/Mog" evidence="4">
        <location>
            <begin position="187"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI48812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="625" mass="64967" checksum="70944861D401112E" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVF1</accession>
    <name>A0A1U7EVF1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1808A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48995.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322627.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVF1"/>
    <dbReference type="STRING" id="348780.NP_1808A"/>
    <dbReference type="EnsemblBacteria" id="CAI48995">
        <property type="protein sequence ID" value="CAI48995"/>
        <property type="gene ID" value="NP_1808A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701260"/>
    <dbReference type="KEGG" id="nph:NP_1808A"/>
    <dbReference type="eggNOG" id="arCOG04523">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131307_0_0_2"/>
    <dbReference type="OrthoDB" id="220637at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48995.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="143" mass="15062" checksum="9711769D9496D0E4" modified="2017-05-10" version="1">MTDAPVLIFDGECPYCSMAAVALRRIEGIRAVSWYDEPAGQFLDAQFGERPFAMILADPAEDTVYAGRSAAEELASRAGTPGIVASLVRDNYDSIAGVVGTLSGREADPDDYHDTYALDAEAAGHLPALRAAGDDAVPDDQPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVF5</accession>
    <name>A0A1U7EVF5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">IS1341-type transposase ISNph19</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_1802A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322624.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVF5"/>
    <dbReference type="STRING" id="348780.NP_1802A"/>
    <dbReference type="EnsemblBacteria" id="CAI48992">
        <property type="protein sequence ID" value="CAI48992"/>
        <property type="gene ID" value="NP_1802A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701258"/>
    <dbReference type="KEGG" id="nph:NP_1802A"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_3_3_2"/>
    <dbReference type="OrthoDB" id="210698at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF26">
        <property type="entry name" value="TRANSPOSASE, PROBABLY IS605-TNPB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas12f1-like TNB" evidence="3">
        <location>
            <begin position="301"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="399"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="402"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="420" mass="47386" checksum="80CE20BDA8B9C211" modified="2017-05-10" version="1">MAVVRNLEIKLDVDEDAHSVLDETFEQFRQAAQHAADYGWSDDPTQIIDSKSKLHDATYSDVREKTDLTANHVQAARSLAANALENCKDRILEDGKKASKPEFRGTVVFYDGRTITYNDDHCTLSTTEDRVTAEYIVPEDTRGTPFEEYWENDEWERGESTLHKRDGTYYLHVSMKKEREEDEASTSEYGAVLGVDLNVDGYLAVTSTGAFLGNADYLNHKRAEYERRRGKLQQTGTRSAHLTLKSIGDRFSNWSEDYLHRVALAVVLEARRHDCDAIAFENLKHIRGRISNASKFQQWAFNKLQSLVEYKAEEYGILVDDVKPQYTSQRCSHSECGFTHEDNRDGDEFECLSCGKELHADYNAARNIGWRLVQHWLKSGAGRADCQVALKSGMVNANSEFSPTTGSSGQSGSPLTSPPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVG3</accession>
    <name>A0A1U7EVG3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1858A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322652.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVG3"/>
    <dbReference type="STRING" id="348780.NP_1858A"/>
    <dbReference type="EnsemblBacteria" id="CAI49020">
        <property type="protein sequence ID" value="CAI49020"/>
        <property type="gene ID" value="NP_1858A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701279"/>
    <dbReference type="KEGG" id="nph:NP_1858A"/>
    <dbReference type="eggNOG" id="arCOG03055">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1425126_0_0_2"/>
    <dbReference type="OrthoDB" id="271327at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="43"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="190" mass="19441" checksum="7A0F836A518DAC09" modified="2017-05-10" version="1">MVSRRQLLAAGGLASAGLAGCLDSLSDGSSPEPEPAETPPTPEYDSPAPVSVADAAPVDADSPLVTVAAEGSPTGTPDGETERLLATYGDVAELGVPAIDETQQTHYVSVLFEAAAADAFVDQLAAIGALESPGDYDLGIVFPDGETAEYDLAPSLAQAMAAGDWDGRFRLQGDDEAAMEAVVDAFDREQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVL8</accession>
    <name>A0A1U7EVL8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ABC-type transport system periplasmic substrate-binding protein (Probable substrate phosphate)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pstS1</name>
        <name evidence="4" type="synonym">abc14s</name>
        <name evidence="4" type="ordered locus">NP_1978A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49080.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVL8"/>
    <dbReference type="STRING" id="348780.NP_1978A"/>
    <dbReference type="EnsemblBacteria" id="CAI49080">
        <property type="protein sequence ID" value="CAI49080"/>
        <property type="gene ID" value="NP_1978A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_1978A"/>
    <dbReference type="eggNOG" id="arCOG00213">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026228_1_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042301">
        <property type="term" value="F:phosphate ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13654">
        <property type="entry name" value="PBP2_phosphate_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024370">
        <property type="entry name" value="PBP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011862">
        <property type="entry name" value="Phos-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050811">
        <property type="entry name" value="Phosphate_ABC_transporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02136">
        <property type="entry name" value="ptsS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30570">
        <property type="entry name" value="PERIPLASMIC PHOSPHATE BINDING COMPONENT OF PHOSPHATE ABC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30570:SF1">
        <property type="entry name" value="PHOSPHATE-BINDING PROTEIN PSTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12849">
        <property type="entry name" value="PBP_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PBP" evidence="3">
        <location>
            <begin position="33"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49080.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="339" mass="37121" checksum="BB7C940320F2AA24" modified="2017-05-10" version="1">MSRRSLLAAAGAGLAALSGCTVLGDPPGEDSGLRGEITMDGSNTVLPHGAAVAEEFLWRNNRVTIPVRGSGTGAGFQQFCRGETDIQNASRPVQESEEQLCTENGVDYIELEALLDGIAIMVDADNDWCDCLTVEELRAMWESGSDVETWSDVRPAWPDEEIDFFGRDTASGTFDSFTERLMGETGNIRNDYSASSDTNVIVRGVSGNRNAIGFGGAGFYYENEDQLRLVGVDDGNGCVRPTRETIENEEYTPLTRPMYVYLSTERLDDPAVQAFARFYFEEIDDAVHESAVEAGIAAPNETLTWTQWAARRVGYYAAPDEVVDESRAKLEQKIAEATE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVM1</accession>
    <name>A0A1U7EVM1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1974A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49078.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939503.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVM1"/>
    <dbReference type="STRING" id="348780.NP_1974A"/>
    <dbReference type="EnsemblBacteria" id="CAI49078">
        <property type="protein sequence ID" value="CAI49078"/>
        <property type="gene ID" value="NP_1974A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701325"/>
    <dbReference type="KEGG" id="nph:NP_1974A"/>
    <dbReference type="eggNOG" id="arCOG00504">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030571_2_4_2"/>
    <dbReference type="OrthoDB" id="197151at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07721">
        <property type="entry name" value="yflN-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050855">
        <property type="entry name" value="NDM-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42951:SF4">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE MBLAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42951">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="16"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49078.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="224" mass="24345" checksum="3D05CA876B2B3FF9" modified="2017-05-10" version="1">MVTELRDGVWWFEPMGVNAYLVADDEGLTLVDAGTPFDGGTVVEAIEAAGFDIADLERILITHYDFDHVGPLSTLAVDTPVYIGQADAPLLTGAERPSPYGIKRLTQLVSGPFVGDLPERRVRPLEDGDTVGGFTAHHAPGHTPGHTVYVHEERSAAFLGDLVIERDGELAPAPWFISRDRDRLEESIADLAARLPDFEVAAMGHGTPFRTGGSERLRALAERY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVX3</accession>
    <name>A0A1U7EVX3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2234A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322835.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVX3"/>
    <dbReference type="EnsemblBacteria" id="CAI49208">
        <property type="protein sequence ID" value="CAI49208"/>
        <property type="gene ID" value="NP_2234A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701386"/>
    <dbReference type="KEGG" id="nph:NP_2234A"/>
    <dbReference type="eggNOG" id="arCOG02454">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1014169_0_0_2"/>
    <dbReference type="OrthoDB" id="109251at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055927">
        <property type="entry name" value="DUF7504"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24336">
        <property type="entry name" value="DUF7504"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="274" mass="30531" checksum="029B46CE522E3E09" modified="2017-05-10" version="1">MVYRWDCRHCAFSAWSNSDERLRKNAGAHLFDHHSSKLSKADFRVAWDCPYCDAGETAHDKGAAAQAFKDHLDWHAGNSIESNAHLADEVENSGNVLVQTAADSAAADSARLQFTARSDLSIIVTKSPKERLRLLHDRFNGWPDRTVVMTTKRRPLAGAFDIDLSDAPVEVVELDRRLGPSQLGETISRVIDAHHTPDQRLAVGFDILYDIVSSFDLQTTHDFVSMLSSRLSEADALWHIYAEPRPQLSTALNVLEEYIDLTVETESGVFVVNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVY6</accession>
    <name>A0A1U7EVY6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">NADH dehydrogenase-like complex subunit A</fullName>
            <ecNumber evidence="8">1.6.5.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">nuoA</name>
        <name evidence="8" type="ordered locus">NP_2292A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I subunit 3 family.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.-" evidence="8"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322864.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVY6"/>
    <dbReference type="STRING" id="348780.NP_2292A"/>
    <dbReference type="DNASU" id="3702989"/>
    <dbReference type="EnsemblBacteria" id="CAI49237">
        <property type="protein sequence ID" value="CAI49237"/>
        <property type="gene ID" value="NP_2292A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702989"/>
    <dbReference type="KEGG" id="nph:NP_2292A"/>
    <dbReference type="eggNOG" id="arCOG01557">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119549_1_1_2"/>
    <dbReference type="OrthoDB" id="9823at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030964">
        <property type="term" value="C:NADH dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1610">
        <property type="entry name" value="NADH:ubiquinone/plastoquinone oxidoreductase, chain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01394">
        <property type="entry name" value="NDH1_NuoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023043">
        <property type="entry name" value="NAD(P)H_OxRDtase_bac/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000440">
        <property type="entry name" value="NADH_UbQ/plastoQ_OxRdtase_su3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038430">
        <property type="entry name" value="NDAH_ubi_oxred_su3_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11058">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11058:SF9">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00507">
        <property type="entry name" value="Oxidored_q4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="60"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="134" mass="14825" checksum="7C99D6CDE517B0C0" modified="2017-05-10" version="1">MSDPWIAVGALAVVGLAIPLSMIVISSLLRPSVPEQGKSATYESGEIPTGDTRIQFNIQYYMVALLFLVFDVETALIFPWALIYSDAVAEFGLVRVLVPMLVFLAILIIGLGWAWRNGAVRWVRSERAEIKTEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW35</accession>
    <name>A0A1U7EW35_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7511 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2378A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW35"/>
    <dbReference type="STRING" id="348780.NP_2378A"/>
    <dbReference type="EnsemblBacteria" id="CAI49280">
        <property type="protein sequence ID" value="CAI49280"/>
        <property type="gene ID" value="NP_2378A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_2378A"/>
    <dbReference type="HOGENOM" id="CLU_2748302_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055933">
        <property type="entry name" value="DUF7511"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24351">
        <property type="entry name" value="DUF7511"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7511" evidence="2">
        <location>
            <begin position="26"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="70" mass="7498" checksum="8EEA5AFF4EB23375" modified="2017-05-10" version="1">MISPAVTDTRNPAQPTRDAPTPVENYRAAVETDDDGTTTCTIYSTAPADTLLTTWISADGDSFCSLAEMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW40</accession>
    <name>A0A1U7EW40_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">UVR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2388A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322911.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW40"/>
    <dbReference type="STRING" id="348780.NP_2388A"/>
    <dbReference type="EnsemblBacteria" id="CAI49285">
        <property type="protein sequence ID" value="CAI49285"/>
        <property type="gene ID" value="NP_2388A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701432"/>
    <dbReference type="KEGG" id="nph:NP_2388A"/>
    <dbReference type="HOGENOM" id="CLU_2839466_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="65" mass="7666" checksum="14E8F71E215FF53E" modified="2017-05-10" version="1">MARDYPKEAPAGVPPEDRERARELQHELVVLKARLESATFDQQEAYRRAIRDRREELRSLVETDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWA4</accession>
    <name>A0A1U7EWA4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2548A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322990.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWA4"/>
    <dbReference type="STRING" id="348780.NP_2548A"/>
    <dbReference type="EnsemblBacteria" id="CAI49365">
        <property type="protein sequence ID" value="CAI49365"/>
        <property type="gene ID" value="NP_2548A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701481"/>
    <dbReference type="KEGG" id="nph:NP_2548A"/>
    <dbReference type="eggNOG" id="arCOG10815">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1901909_0_0_2"/>
    <dbReference type="OrthoDB" id="202653at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="141" mass="14715" checksum="C860A3048B62DEDB" modified="2017-05-10" version="1">MRTDSDSPWLLGGLCLIAVAAAGILHAVYIPRHIPGSFSRALPYLVVGWASYAFVFYALGRLGPLASGMPSMRALDFGLGLFLFSIVVSGLFDAAGLTLTVAPGLHLLPALGLYVGLALAGWGFGARTRAVNRIAAEAERG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWE9</accession>
    <name>A0A1U7EWE9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HMA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2666A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323049.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWE9"/>
    <dbReference type="STRING" id="348780.NP_2666A"/>
    <dbReference type="EnsemblBacteria" id="CAI49424">
        <property type="protein sequence ID" value="CAI49424"/>
        <property type="gene ID" value="NP_2666A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701519"/>
    <dbReference type="KEGG" id="nph:NP_2666A"/>
    <dbReference type="eggNOG" id="arCOG02764">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134973_5_3_2"/>
    <dbReference type="OrthoDB" id="44171at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMA" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="64" mass="6505" checksum="278182B2BC5FDDCF" modified="2017-05-10" version="1">MATTLSVPDMACGGCETEIEESLGELDGVDAVDADHEDGTVTVEGGADWNVLAEAVSEAGYSVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWG8</accession>
    <name>A0A1U7EWG8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Enoyl-CoA hydratase</fullName>
            <ecNumber evidence="3">4.2.1.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">fadA1</name>
        <name evidence="3" type="ordered locus">NP_2632A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.17" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323032.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWG8"/>
    <dbReference type="STRING" id="348780.NP_2632A"/>
    <dbReference type="EnsemblBacteria" id="CAI49407">
        <property type="protein sequence ID" value="CAI49407"/>
        <property type="gene ID" value="NP_2632A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702680"/>
    <dbReference type="KEGG" id="nph:NP_2632A"/>
    <dbReference type="eggNOG" id="arCOG00239">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_6_2"/>
    <dbReference type="OrthoDB" id="27846at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000009">
        <property type="entry name" value="Carnitinyl-CoA dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.12.10:FF:000001">
        <property type="entry name" value="Probable enoyl-CoA hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941:SF54">
        <property type="entry name" value="ENOYL-COA HYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941">
        <property type="entry name" value="ENOYL-COA HYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="273" mass="28960" checksum="632A6FBEF1BD8380" modified="2017-05-10" version="1">MSDEALDGLDDEFELVSIDVADTVKKVVTVSIERPDARNALNSQVRTELKRAVAAAEADDDVRVLVLTGGEGSGAFVAGADITEFKDRDLVEQREASERPRIYETVDDASIPVIARINGHALGGGCELAQACDIRIAAEGAKLGQPEINLGLIPGGGGTQRLARLVGEGQAMRLILSGELIDASEAKDIGLVDEVYAEENLDEGVYDLAGSIAQKSPVALELAKDAIKAGSRMGLEEGLDYESELFVQLFATADKDEGIQAFLDGRDPEFTGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWI1</accession>
    <name>A0A1U7EWI1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TraB family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2702A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323067.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWI1"/>
    <dbReference type="STRING" id="348780.NP_2702A"/>
    <dbReference type="EnsemblBacteria" id="CAI49442">
        <property type="protein sequence ID" value="CAI49442"/>
        <property type="gene ID" value="NP_2702A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701530"/>
    <dbReference type="KEGG" id="nph:NP_2702A"/>
    <dbReference type="eggNOG" id="arCOG02142">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032780_1_0_2"/>
    <dbReference type="OrthoDB" id="185689at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd14726">
        <property type="entry name" value="TraB_PrgY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002816">
        <property type="entry name" value="TraB/PrgY/GumN_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046345">
        <property type="entry name" value="TraB_PrgY-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21530">
        <property type="entry name" value="PHEROMONE SHUTDOWN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21530:SF7">
        <property type="entry name" value="TRAB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01963">
        <property type="entry name" value="TraB_PrgY_gumN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="316"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="347"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="379"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="440"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="501" mass="53504" checksum="B0FD446E845D2064" modified="2017-05-10" version="1">MSTDVEEGSVTVVGTAHVSEASVEEVEAVIDEERPDVVAVELDEGRYRQLRGEAPEDLDAGDLLKGNTVYQFLAYWMLSYVQARLGDRFDIEPGADMKAAVDTAEAYGIDVALVDRDIQVTIQRFWARLSFFEKLKLVGSLAVGFGTPVEIGMGVGIGIALVVSILAGAVAGPFVVPAGLGPGILVGAADTLLVALGLGLVLGLAITALLGRLAPEDDELQEFDVDRMTDTDVVSAMMEEFRRFSPGGAEALIDERDAYIAHNLVSLRDQGHDVVAVVGAGHRKGIEKYLETPSLLPPMEDLTGKASGSRFSLYKLFGYLFTLGFLAFFVLLAIATYTGVEGASSELLFRLFAAWFLVNGALAAGLAKFAGAHWRSAGVGGAVAWLTSVNPLLAPGWFAGYVELRYLNVNVGDISTLNELMSDEETPLRQLWSDLTDVPLFRLIMIVALTNVGSFIGSIVFATVFLPYLFAGSGVEGASGVARLMVEAARNSVDIIFGSFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWM0</accession>
    <name>A0A1U7EWM0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Probable secreted glycoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2816A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323124.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWM0"/>
    <dbReference type="STRING" id="348780.NP_2816A"/>
    <dbReference type="EnsemblBacteria" id="CAI49499">
        <property type="protein sequence ID" value="CAI49499"/>
        <property type="gene ID" value="NP_2816A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701568"/>
    <dbReference type="KEGG" id="nph:NP_2816A"/>
    <dbReference type="eggNOG" id="arCOG07781">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_653181_0_0_2"/>
    <dbReference type="OrthoDB" id="248699at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00146">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022409">
        <property type="entry name" value="PKD/Chitinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000601">
        <property type="entry name" value="PKD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035986">
        <property type="entry name" value="PKD_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22352">
        <property type="entry name" value="K319L-like_PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00089">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49299">
        <property type="entry name" value="PKD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50093">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PKD" evidence="2">
        <location>
            <begin position="25"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="420" mass="44710" checksum="FFC8881288B11CF1" modified="2017-05-10" version="1">MHWRITIAVGIVAVAALSGVAAADEPMADAGLDQEVSVNTTVQLDGTGSTHPDGEIQTYQWDVTHDGGTTVYTSCMTCSRTEFTPSAPGTYEVTLSVTDGASQRATDTLYVTVTDAGPSVELDGPLTVDTDDPATFEAAAESPDAELETVSWAVEDDIVTERPLDGGVDTDAFQFAFGDPDTYRLQVVVRDVNGRTTYEELFVQAEAPPEPSGGSSGGSSGGTETPTPEETPEETPRIGDPNNEESVIYEADGYYQNRNAGADVADSAYIGTEVTEYSFDDGENAPWGQPIMEQAGGSAWEGIQSQVYGEERQEHSCTITGDSIDMCSQVAHQIESHGETSHVYSPDKSGTYHEYGLRDVERTEGPNPLENRDEVDKIHVTIITQPEEDGLVDQASDIADGVVDGVDSAFGYVRSGGESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWX5</accession>
    <name>A0A1U7EWX5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ModE family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">abc22u</name>
        <name evidence="4" type="ordered locus">NP_3046A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49614.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWX5"/>
    <dbReference type="STRING" id="348780.NP_3046A"/>
    <dbReference type="EnsemblBacteria" id="CAI49614">
        <property type="protein sequence ID" value="CAI49614"/>
        <property type="gene ID" value="NP_3046A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3046A"/>
    <dbReference type="eggNOG" id="arCOG00223">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072980_0_0_2"/>
    <dbReference type="OrthoDB" id="70912at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015689">
        <property type="term" value="P:molybdate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051815">
        <property type="entry name" value="Molybdate_resp_trans_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004606">
        <property type="entry name" value="Mop_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005116">
        <property type="entry name" value="Transp-assoc_OB_typ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30432:SF1">
        <property type="entry name" value="DNA-BINDING TRANSCRIPTIONAL DUAL REGULATOR MODE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30432">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR MODE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03459">
        <property type="entry name" value="TOBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="MOP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51866">
        <property type="entry name" value="MOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mop" evidence="3">
        <location>
            <begin position="164"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49614.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="230" mass="24110" checksum="2390E9176EF10FF5" modified="2017-05-10" version="1">MNRTNGQGEAALVAEGVEFDADDATLLREINETGSVARASSNLGRSRARQLSRIETLESAFGDLVERRRGGSGGGGSRLTENAGALLRRYDRLQTALTATDTVPETVLNGTVTAVLGELADVETEIGTVRGLHPDVTAGDDVQVRIGADAVTLQDPADSPGPDATSARNRYQGTVTAVEHGETVLEVTVAVSDVPFRALVTDESADRLGLDAGCPVVLTWKATATRLALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX11</accession>
    <name>A0A1U7EX11_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Small CPxCG-related zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3084A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323255.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX11"/>
    <dbReference type="EnsemblBacteria" id="CAI49633">
        <property type="protein sequence ID" value="CAI49633"/>
        <property type="gene ID" value="NP_3084A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701647"/>
    <dbReference type="KEGG" id="nph:NP_3084A"/>
    <dbReference type="eggNOG" id="arCOG01917">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1269964_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059113">
        <property type="entry name" value="Znf_ribbon"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13248">
        <property type="entry name" value="Zn_ribbon_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Putative zinc-ribbon" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="217" mass="22445" checksum="B33CA9B298717E70" modified="2017-05-10" version="1">MQQCSNCGESVDADDTFCPNCGAQFQSAAPDSSLQSSSVETSPDADAAGASSDDWTLTHYAGIALSLAAAGGIVALATMDVFQRFDPGAVEWLFLLLWFGAASGIVLRDSRGIGLAAVIFAAGGVVSGLGFVQRLLWEVAPELATELFNTTVPAPDPVMGIHNLLLGVLWSPTGGGSTVSTVLLDIEIATHLFGVLVCFIVATVLWRNRSALATSAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX12</accession>
    <name>A0A1U7EX12_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF8149 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3120A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323273.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX12"/>
    <dbReference type="EnsemblBacteria" id="CAI49651">
        <property type="protein sequence ID" value="CAI49651"/>
        <property type="gene ID" value="NP_3120A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701658"/>
    <dbReference type="KEGG" id="nph:NP_3120A"/>
    <dbReference type="eggNOG" id="arCOG06326">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2662245_0_0_2"/>
    <dbReference type="OrthoDB" id="260707at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058462">
        <property type="entry name" value="DUF8149"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26476">
        <property type="entry name" value="DUF8149"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8149" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="69" mass="7713" checksum="A5DE9E2235EDC896" modified="2017-05-10" version="1">MTDEDPPEIPILCSECDTRTKIPFPEVEATVERHNEQLHDGEPVAQVDPAVFDELADFVAEDLGLYEDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX91</accession>
    <name>A0A1U7EX91_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Small CPxCG-related zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3378A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49780.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939598.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX91"/>
    <dbReference type="STRING" id="348780.NP_3378A"/>
    <dbReference type="EnsemblBacteria" id="CAI49780">
        <property type="protein sequence ID" value="CAI49780"/>
        <property type="gene ID" value="NP_3378A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701756"/>
    <dbReference type="KEGG" id="nph:NP_3378A"/>
    <dbReference type="eggNOG" id="arCOG04600">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2662225_0_0_2"/>
    <dbReference type="OrthoDB" id="165929at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043813">
        <property type="entry name" value="DUF5795"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19108">
        <property type="entry name" value="DUF5795"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49780.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="74" mass="8194" checksum="EF3A4F8270788FA1" modified="2017-05-10" version="1">MSENRVVEGRMVTPQKLAELIEDDSVMDTEGIEDADRQCPECGGDVLSVGYMPGITEFVTGYKCQDCGWSETDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXD5</accession>
    <name>A0A1U7EXD5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3494A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323458.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXD5"/>
    <dbReference type="STRING" id="348780.NP_3494A"/>
    <dbReference type="EnsemblBacteria" id="CAI49838">
        <property type="protein sequence ID" value="CAI49838"/>
        <property type="gene ID" value="NP_3494A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701789"/>
    <dbReference type="KEGG" id="nph:NP_3494A"/>
    <dbReference type="eggNOG" id="arCOG11332">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_935727_0_0_2"/>
    <dbReference type="OrthoDB" id="385771at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="308" mass="33181" checksum="5F128BE579724ADD" modified="2017-05-10" version="1">MQRDYRYTVYVLALAALVTLATAGLTVAGSASTSDPADASLAETDFEIVDHDDTLTDEETDEISDLVRETEPLQEHFDGVDALQLDIHQSKALDDGAFVPIEGEYTVKLSPPSDDRPLPSASLSVNVDESALVIDRTIAPIDDVNVTVEDDGHLSDDEIDQLTGALPDDGDVAYQIQTFLGEPDTVEMAVTDRDGDEVDADVTVDGASDTVHVTVDIDEETVLSNHVAMQLDSDELDEEDDLTLNVDMSADTDTDGAADESDETDAEAENVDTDEREEITVEIVDDDGLEDAGDEFTFTVVTEDDGDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXE1</accession>
    <name>A0A1U7EXE1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3496A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323459.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXE1"/>
    <dbReference type="STRING" id="348780.NP_3496A"/>
    <dbReference type="EnsemblBacteria" id="CAI49839">
        <property type="protein sequence ID" value="CAI49839"/>
        <property type="gene ID" value="NP_3496A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701790"/>
    <dbReference type="KEGG" id="nph:NP_3496A"/>
    <dbReference type="eggNOG" id="arCOG06152">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1006874_0_0_2"/>
    <dbReference type="OrthoDB" id="186743at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="247" mass="26358" checksum="8BE2B076C03D7EFF" modified="2017-05-10" version="1">MSDRETDDIRSTRELTREELHTTFEYQVSRIQEIDAKAVEILKANLLLIGILVTAGSVLVQTEFEAAVFLNVFTVTGGLLLFVSTGLAGVTYTASNLRGGLDAAAVEQALTTDSDEAFEERLLRSYGRWIEYNAQVTAVNDMLASATVLLVIVSFAYVVAGIAVGGAALGTVATVGLFIAFTIPVVWLVRLAYHMDHLGGAFQDADDDSFSGVRISKGATRGDGLASLKRILFGSPPDSGSDDESGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXK0</accession>
    <name>A0A1U7EXK0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">FkbM family methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3626A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323524.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXK0"/>
    <dbReference type="STRING" id="348780.NP_3626A"/>
    <dbReference type="EnsemblBacteria" id="CAI49904">
        <property type="protein sequence ID" value="CAI49904"/>
        <property type="gene ID" value="NP_3626A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701829"/>
    <dbReference type="KEGG" id="nph:NP_3626A"/>
    <dbReference type="eggNOG" id="arCOG01402">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081183_0_0_2"/>
    <dbReference type="OrthoDB" id="275825at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006342">
        <property type="entry name" value="FkbM_mtfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052514">
        <property type="entry name" value="SAM-dependent_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01444">
        <property type="entry name" value="fkbM_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34203">
        <property type="entry name" value="METHYLTRANSFERASE, FKBM FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34203:SF15">
        <property type="entry name" value="SLL1173 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05050">
        <property type="entry name" value="Methyltransf_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase FkbM" evidence="1">
        <location>
            <begin position="94"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="263" mass="28560" checksum="314085914D6347F8" modified="2017-05-10" version="1">MGFDGRLYTAYLEPGLDRLGVRTHLSSLYWALGVPGMLFRLSSQETATHAVNGVTVEFPIETRWQYERFRWMHPEIRLFEELVDELGPGDVFYDVGAHLGWHSVVAANAADDVTVEAFEPHPQVADRLRTVVDATGHSIAVRECALADRDGTAEFEAEPTPAAALSGAQDDPPASTVEVALAAGDSLVAEGAVEPPDILKIDAEGADAAVLSGLQETIAAHRPRRIYCEVHVDGDEIRELLSSFGYSFEPIASTRPVLRAVPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXK9</accession>
    <name>A0A1U7EXK9_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF8152 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3612A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323517.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXK9"/>
    <dbReference type="EnsemblBacteria" id="CAI49897">
        <property type="protein sequence ID" value="CAI49897"/>
        <property type="gene ID" value="NP_3612A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701823"/>
    <dbReference type="KEGG" id="nph:NP_3612A"/>
    <dbReference type="eggNOG" id="arCOG10156">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_162433_0_0_2"/>
    <dbReference type="OrthoDB" id="204708at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058465">
        <property type="entry name" value="DUF8152"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26479">
        <property type="entry name" value="DUF8152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8152" evidence="1">
        <location>
            <begin position="9"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="94" mass="10234" checksum="36924EC7FA0E6B99" modified="2017-05-10" version="1">MDKEPPAARVEQLHEELAATQELPVERTASRWIGEAEAVAGDLVGVDSDSDLVYRRVSHVVDLLANVDETGDDTADQHLAEAKRLAAEVVELTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXL7</accession>
    <name>A0A1U7EXL7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Thioredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">trxA3</name>
        <name evidence="4" type="ordered locus">NP_3704A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49943.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939625.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXL7"/>
    <dbReference type="STRING" id="348780.NP_3704A"/>
    <dbReference type="EnsemblBacteria" id="CAI49943">
        <property type="protein sequence ID" value="CAI49943"/>
        <property type="gene ID" value="NP_3704A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703424"/>
    <dbReference type="KEGG" id="nph:NP_3704A"/>
    <dbReference type="eggNOG" id="arCOG01972">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090389_10_4_2"/>
    <dbReference type="OrthoDB" id="35385at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005746">
        <property type="entry name" value="Thioredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01068">
        <property type="entry name" value="thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43601">
        <property type="entry name" value="THIOREDOXIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43601:SF3">
        <property type="entry name" value="THIOREDOXIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="3">
        <location>
            <begin position="16"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49943.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="128" mass="14073" checksum="76931123C481F73F" modified="2017-05-10" version="1">MSDDDLEAIREQKREALMSRAATPDEPVSVEGPGHLEERLDEHETVLVDFYADWCGPCKMLAPKLSAVAAETDAAVLKVDIDANQALASEYQVQGVPTLYLFHNGDVEDRMVGMQDEATLKQKIAAVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXM7</accession>
    <name>A0A1U7EXM7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DHH/RecJ family phosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3708A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323563.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXM7"/>
    <dbReference type="STRING" id="348780.NP_3708A"/>
    <dbReference type="EnsemblBacteria" id="CAI49945">
        <property type="protein sequence ID" value="CAI49945"/>
        <property type="gene ID" value="NP_3708A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701845"/>
    <dbReference type="KEGG" id="nph:NP_3708A"/>
    <dbReference type="eggNOG" id="arCOG01566">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046377_1_1_2"/>
    <dbReference type="OrthoDB" id="350705at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1640.10">
        <property type="entry name" value="inorganic pyrophosphatase (n-terminal core)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051319">
        <property type="entry name" value="Oligoribo/pAp-PDE_c-di-AMP_PDE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47618">
        <property type="entry name" value="BIFUNCTIONAL OLIGORIBONUCLEASE AND PAP PHOSPHATASE NRNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47618:SF1">
        <property type="entry name" value="BIFUNCTIONAL OLIGORIBONUCLEASE AND PAP PHOSPHATASE NRNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="DHH phosphoesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RCK N-terminal" evidence="1">
        <location>
            <begin position="10"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="484" mass="51970" checksum="0432918DD0D5E122" modified="2017-05-10" version="1">MSSAISMASMSSYAILGCGSVGHAVAEELVEEGKDVLILDRDEGRVEALRDQDLDARTADIEDPTAAEAIADRDVVLILSPDVEANRVAVENLREHAEDKFIIVRASDPVTADELKEAGADVVINPSAVIADSALRALEQGELEHKAAQLADIIDDGETLAVLAHRSPGPDSIASAVALQAIAEARDVEADILYEGEIGHQENRAFVNLLGIELVSLGETDLEEYDTLALVDCAKATEPVVDGEVDILIDHFEPEVDYEAEFNDIRPNVSSTSTILTKYIQEFDLSLSEEVATALLYGIRAETLDFKRDTTPADLTAAAYLYPFANHDTLEQVESPSMSPETLDVLAEAIRNREVKGSHLISNAGVIRDRDALSQASQHLLNLEGITTSAVFAIADDTIYLAARSKDIRINIGNVLQDAFGEIGDVRGHSTDASAEIPLGIFTGLEITGDNRDTLLELTEQAVRTRLFEAMGVEGSSGDSSNSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXT5</accession>
    <name>A0A1U7EXT5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DUF2800 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3854A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323635.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXT5"/>
    <dbReference type="STRING" id="348780.NP_3854A"/>
    <dbReference type="EnsemblBacteria" id="CAI50018">
        <property type="protein sequence ID" value="CAI50018"/>
        <property type="gene ID" value="NP_3854A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701888"/>
    <dbReference type="KEGG" id="nph:NP_3854A"/>
    <dbReference type="eggNOG" id="arCOG04505">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071988_0_0_2"/>
    <dbReference type="OrthoDB" id="247969at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058289">
        <property type="entry name" value="DUF7983"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25943">
        <property type="entry name" value="DUF7983"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="279"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="322" mass="37105" checksum="1A6CF23891A25012" modified="2017-05-10" version="1">MPFTASWHTLLDELEDLPDGATLITPLSHKRFRITDVQEHRVIIEFEDADEKRPLQRDQFETLYRRVSDEPEGFELDRLPSDADPYPAVLSLHPRFEIDEDNGVIVKTEKPTSDQLLGSDSESTSEERTEPDLNIYADALLLVDALERHDVATLDDMETDGLVNLYTLLSDVQRNANEFRKNVADVLLNRLHHDRPVSGPYGSVQRTTRRNRTLKDEQEVLEILEEAGIDQERVMSVDRDKIDEALEVTELSESGVYEIDEREYVRKAEVDEEVKQTRLQGLKEQLAATEGEEADQLRQEIEELEDRIDELTSFRTGTEIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXY0</accession>
    <name>A0A1U7EXY0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">IS200-type transposase ISNph6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3910A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323663.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXY0"/>
    <dbReference type="EnsemblBacteria" id="CAI50046">
        <property type="protein sequence ID" value="CAI50046"/>
        <property type="gene ID" value="NP_3910A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701914"/>
    <dbReference type="KEGG" id="nph:NP_3910A"/>
    <dbReference type="eggNOG" id="arCOG02759">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101320_2_0_2"/>
    <dbReference type="OrthoDB" id="92826at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1290">
        <property type="entry name" value="Transposase IS200-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002686">
        <property type="entry name" value="Transposase_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036515">
        <property type="entry name" value="Transposase_17_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033573">
        <property type="entry name" value="transpos_IS200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33360">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33360:SF2">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01797">
        <property type="entry name" value="Y1_Tnp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01321">
        <property type="entry name" value="Y1_Tnp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143422">
        <property type="entry name" value="Transposase IS200-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS200-like" evidence="1">
        <location>
            <begin position="11"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="135" mass="15905" checksum="961046338E34279B" modified="2017-05-10" version="1">MEEYRSHAHSVSSCKYHFVWCRKYRHQILNAVEDDVRELFDETADHFGHEILALEIADDHVHLFVQTDSKYSPANIARQFKSYSGKHLLERYPEIRESYFWGGGFWKVGYYVGTTGAVSEEVVERYIEETEHAPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXY1</accession>
    <name>A0A1U7EXY1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3938A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323677.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXY1"/>
    <dbReference type="STRING" id="348780.NP_3938A"/>
    <dbReference type="EnsemblBacteria" id="CAI50060">
        <property type="protein sequence ID" value="CAI50060"/>
        <property type="gene ID" value="NP_3938A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701917"/>
    <dbReference type="KEGG" id="nph:NP_3938A"/>
    <dbReference type="eggNOG" id="arCOG06190">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_152990_1_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055894">
        <property type="entry name" value="DUF7471"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24283">
        <property type="entry name" value="DUF7471"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="114" mass="12088" checksum="87FCA9953993E4BB" modified="2017-05-10" version="1">MLTSQLSVADPWWGVEGSMLILGVILLAALGTAVLFLISLAAYLQRRTLQYGLITVAIGLLLLRSVVGAGTALGRVPMPVHHLVEHGLDFAIAGLLLFAIYISYSSTGSDPEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY15</accession>
    <name>A0A1U7EY15_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">XapX family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4056A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323735.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY15"/>
    <dbReference type="STRING" id="348780.NP_4056A"/>
    <dbReference type="EnsemblBacteria" id="CAI50119">
        <property type="protein sequence ID" value="CAI50119"/>
        <property type="gene ID" value="NP_4056A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701952"/>
    <dbReference type="KEGG" id="nph:NP_4056A"/>
    <dbReference type="eggNOG" id="arCOG06356">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_171061_3_0_2"/>
    <dbReference type="OrthoDB" id="305804at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020017">
        <property type="entry name" value="XapX_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03510">
        <property type="entry name" value="XapX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="66" mass="6505" checksum="A6324456F7E008C4" modified="2017-05-10" version="1">MEASLLIAALALLTGFLAGAVFALVGVPIPAPPTLAGVLGIVGIYLGFKSVDYLGVSYDVAGSLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY22</accession>
    <name>A0A1U7EY22_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aminotransferase</fullName>
            <ecNumber evidence="7">2.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">aspC1</name>
        <name evidence="9" type="ordered locus">NP_4024A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323719.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY22"/>
    <dbReference type="STRING" id="348780.NP_4024A"/>
    <dbReference type="EnsemblBacteria" id="CAI50103">
        <property type="protein sequence ID" value="CAI50103"/>
        <property type="gene ID" value="NP_4024A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702533"/>
    <dbReference type="KEGG" id="nph:NP_4024A"/>
    <dbReference type="eggNOG" id="arCOG01130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_4_3_2"/>
    <dbReference type="OrthoDB" id="372018at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000033">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050596">
        <property type="entry name" value="AspAT/PAT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383:SF3">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 9" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="8">
        <location>
            <begin position="29"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI50103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="380" mass="42018" checksum="0F1D32B03A0041D5" modified="2017-05-10" version="1">MTLEPSDRVADVPPSGIRRFFELAEEMDDVISLGVGEPDFSAPWAAREAAISSLERGRTSYTANRGKRELREEIADDVRRWNLQYDPDDEILVTAGASEAIDLAFRALVNPGDTVAVVQPCYVSYTPGVTFAGGEVLDVPTRAEDEFKLTQEVLYEEGADEADLLVYCYPNNPTGATMNREELAEVAAFCREEDVDVLADEIYADLTYDGDHVSIAEFDGMRERTVVFNGFSKAYAMTGLRLGYALGPADVISAMNRIHQYTMLSAPTTAQHAAIEALQTCREDVEEMRDQYDRRRNFVLSRFEEMGIDCFEATGAFYVFPKCPGDDAGQFAEDLLEAEQVAMVPGDAFGAGASGHLRASYATGLDELREAMDRLERFVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY40</accession>
    <name>A0A1U7EY40_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aminopyrimidine aminohydrolase</fullName>
            <ecNumber evidence="2">3.5.99.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">tenA1</name>
        <name evidence="2" type="ordered locus">NP_4080A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.99.2" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323747.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY40"/>
    <dbReference type="STRING" id="348780.NP_4080A"/>
    <dbReference type="EnsemblBacteria" id="CAI50131">
        <property type="protein sequence ID" value="CAI50131"/>
        <property type="gene ID" value="NP_4080A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701963"/>
    <dbReference type="KEGG" id="nph:NP_4080A"/>
    <dbReference type="eggNOG" id="arCOG01128">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077537_0_1_2"/>
    <dbReference type="OrthoDB" id="196770at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050334">
        <property type="term" value="F:thiaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd19358">
        <property type="entry name" value="TenA_E_Spr0628-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.910.10">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016084">
        <property type="entry name" value="Haem_Oase-like_multi-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026285">
        <property type="entry name" value="TenA_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004305">
        <property type="entry name" value="Thiaminase-2/PQQC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050967">
        <property type="entry name" value="Thiamine_Salvage_TenA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43198">
        <property type="entry name" value="BIFUNCTIONAL TH2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43198:SF2">
        <property type="entry name" value="SI:CH1073-67J19.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03070">
        <property type="entry name" value="TENA_THI-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003170">
        <property type="entry name" value="Pet18p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48613">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiaminase-2/PQQC" evidence="1">
        <location>
            <begin position="27"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="226" mass="26314" checksum="B36FF71EFAB1C676" modified="2017-05-10" version="1">MSRSTADSTYDPAEYDRFTEWLRDRSDWEEATRHRFVEAYRTGDLSDDVFEQYLVQDYQFLETGARVTALAVNQAHTMEEMGQLTESLAVLTGGENDYFQRAFDALSVPGADRERPEIHPTTAAFNDFMLRAANEGTYEETLAAIAPAEWVYLDWCEHAEADHERWYLDEWVEIHVNEEFEAYVDWLRTQLDTYGPQLSPERQRRVAEIFDRTVALEAAFFDAAYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY51</accession>
    <name>A0A1U7EY51_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">NP_4094A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323754.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY51"/>
    <dbReference type="STRING" id="348780.NP_4094A"/>
    <dbReference type="EnsemblBacteria" id="CAI50138">
        <property type="protein sequence ID" value="CAI50138"/>
        <property type="gene ID" value="NP_4094A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701969"/>
    <dbReference type="KEGG" id="nph:NP_4094A"/>
    <dbReference type="eggNOG" id="arCOG01190">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1052133_0_0_2"/>
    <dbReference type="OrthoDB" id="192798at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018934">
        <property type="entry name" value="RIO_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01163">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RIO-type" evidence="9">
        <location>
            <begin position="97"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI50138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="273" mass="29479" checksum="47499FD45F892ED7" modified="2017-05-10" version="1">MPLRRLLRASVPHEQLEAIAAEVARRFDGGDPEAVDIELLEADNWLSVPCVVDDQWFVKVIGDQHTLMHALLTTGRNIGAFSAGTEGFFERFASPVEMAEHELAATQRTRELGVAAPEPIAAFEHDGYGVLVLEYLPDFRTFDALSPARAAEFAPTLFENLATLHAAGLAHGDLRAENVLVAPDADGIETLYFIDATHVSEGAIEDARGYDIACALASLSPLVGPREAVTAATAVYSSAELLAAREFLDFVALRPDHDFDAAALKGELEKRAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY64</accession>
    <name>A0A1U7EY64_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type transport system ATP-binding protein (Probable substrate branched-chain amino acids)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">livF1</name>
        <name evidence="7" type="synonym">abc05a1</name>
        <name evidence="7" type="ordered locus">NP_4138A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323776.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY64"/>
    <dbReference type="STRING" id="348780.NP_4138A"/>
    <dbReference type="EnsemblBacteria" id="CAI50160">
        <property type="protein sequence ID" value="CAI50160"/>
        <property type="gene ID" value="NP_4138A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702377"/>
    <dbReference type="KEGG" id="nph:NP_4138A"/>
    <dbReference type="eggNOG" id="arCOG00924">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015807">
        <property type="term" value="P:L-amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03224">
        <property type="entry name" value="ABC_TM1139_LivF_branched"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052156">
        <property type="entry name" value="BCAA_Transport_ATP-bd_LivF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43820">
        <property type="entry name" value="HIGH-AFFINITY BRANCHED-CHAIN AMINO ACID TRANSPORT ATP-BINDING PROTEIN LIVF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43820:SF4">
        <property type="entry name" value="HIGH-AFFINITY BRANCHED-CHAIN AMINO ACID TRANSPORT ATP-BINDING PROTEIN LIVF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="20"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI50160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="287" mass="31401" checksum="B1C3DAF98CA85CF1" modified="2017-05-10" version="1">MSDDATVGTETMTTEGESILEIRSLDAGYGDLQILSDVDLDVDDGEYVTIVGPNGAGKSTVMKSVFGLTTHMGGTVTFDDEEIQDHSPERIIRTGIGFVPQTDNVFGGLTVRENLEMGAYILEATPADLAEQYPRTAGQLSQSSLESLCRWVPLHRVLGDSVPQDRIDTIYDRFPILEERKHQKAGTLSGGQRQMVAMGRALMLDPDLLLLDEPSAGLAPDLVEDMFDRIDRINDAGTAILMVEQNAKEALRRCDRGYVLVDGQNRYMDSGDALLADDQVRQDFLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY69</accession>
    <name>A0A1U7EY69_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DNA N-glycosylase</fullName>
            <ecNumber evidence="5">3.2.2.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_4170A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the alkylbase DNA glycosidase AlkA family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.-" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323792.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY69"/>
    <dbReference type="STRING" id="348780.NP_4170A"/>
    <dbReference type="EnsemblBacteria" id="CAI50176">
        <property type="protein sequence ID" value="CAI50176"/>
        <property type="gene ID" value="NP_4170A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701993"/>
    <dbReference type="KEGG" id="nph:NP_4170A"/>
    <dbReference type="eggNOG" id="arCOG00464">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_72_5_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032993">
        <property type="term" value="C:protein-DNA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032131">
        <property type="term" value="F:alkylated DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008725">
        <property type="term" value="F:DNA-3-methyladenine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043916">
        <property type="term" value="F:DNA-7-methylguanine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006307">
        <property type="term" value="P:DNA alkylation repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.30:FF:000004">
        <property type="entry name" value="DNA-3-methyladenine glycosylase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051912">
        <property type="entry name" value="Alkylbase_DNA_Glycosylase/TA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43003">
        <property type="entry name" value="DNA-3-METHYLADENINE GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43003:SF5">
        <property type="entry name" value="DNA-3-METHYLADENINE GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="4">
        <location>
            <begin position="41"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI50176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="203" mass="22796" checksum="3C3B7F2B29197921" modified="2017-05-10" version="1">MTAPHDTLRADPVLEPLIERHGALTIEPADDLFRRLLVSVLRQQVSMASAEATKKRLFDAVEPTPTAVLAADTETFREAGLSRQKATYLHNIAAAFEDHGYDRAYFEPMDDEAVRAELTDITGVGEWTANMQLLFSLGREDVFPVGDLGIRKGMRALLDEDLDRAAMTEAAERWAPYRSYASLYLWRVNEPLDESVAEVVRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY78</accession>
    <name>A0A1U7EY78_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Auxin permease family transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">NP_4126A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the auxin efflux carrier (TC 2.A.69) family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323770.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY78"/>
    <dbReference type="STRING" id="348780.NP_4126A"/>
    <dbReference type="EnsemblBacteria" id="CAI50154">
        <property type="protein sequence ID" value="CAI50154"/>
        <property type="gene ID" value="NP_4126A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701982"/>
    <dbReference type="KEGG" id="nph:NP_4126A"/>
    <dbReference type="eggNOG" id="arCOG04756">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056175_3_0_2"/>
    <dbReference type="OrthoDB" id="270046at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004776">
        <property type="entry name" value="Mem_transp_PIN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36838">
        <property type="entry name" value="AUXIN EFFLUX CARRIER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36838:SF4">
        <property type="entry name" value="AUXIN EFFLUX CARRIER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03547">
        <property type="entry name" value="Mem_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="120"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="218"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="250"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI50154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="302" mass="31043" checksum="D2744E8801D0A351" modified="2017-05-10" version="1">MEVLVRLLGLLVLLLAGTGLRASGVLNASRTDRLNALAYYVALPALVFVSTYTRSLVELLSGALFVGLLAVFFATAGLAWVIHRGREANERWSVAVIQSYHSNLGYLGLPLVAATFGGDVTAIASVVLGVVSLMQIMLTVVILSTVNGASTTVGRELSRLLKNPVLAALVAGIAVGTAGVPIPAPAATGLEYLGSLALPIALLCVGASLRVELGDADFVATAAIVALKLGCMPALAWVVFTGLAVEPATLTATVVMFGTPTAVSTYVFASELGGDEQFASLNVFLTTLLSIGTLFILIEAVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYD3</accession>
    <name>A0A1U7EYD3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DUF2342 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4302A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323858.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYD3"/>
    <dbReference type="STRING" id="348780.NP_4302A"/>
    <dbReference type="EnsemblBacteria" id="CAI50242">
        <property type="protein sequence ID" value="CAI50242"/>
        <property type="gene ID" value="NP_4302A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702025"/>
    <dbReference type="KEGG" id="nph:NP_4302A"/>
    <dbReference type="eggNOG" id="arCOG04607">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059556_0_0_2"/>
    <dbReference type="OrthoDB" id="339796at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.150.30">
        <property type="entry name" value="Zincin-like metallopeptidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022454">
        <property type="entry name" value="CHP03883_F420-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018766">
        <property type="entry name" value="Zinicin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042271">
        <property type="entry name" value="Zinicin_2_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03883">
        <property type="entry name" value="DUF2342_F420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03624">
        <property type="entry name" value="putative hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39420:SF1">
        <property type="entry name" value="HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10103">
        <property type="entry name" value="Zincin_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="314" mass="34185" checksum="4C258050D98729DF" modified="2017-05-10" version="1">MSLFRAACAVADAEGDGAIDWSAVGNAARSATDSGELSLSTAERQGYADDVADARDGIRAAAGVDFDLPDTVQVQTRHHWIDANVDTFQRLLGPLEAQVSATPSLARTANTGSMAVTLGFLARNVLGQYDPLLLGDDDHELYFVHPNIEAVADALEVDRDRFRRWIAFHEVAHAAEFGSAPWLADHLESQMEAAVSDLASGSLDRDALGELNVTMTAVEGYAELVMDRAFDDEYADLRAKLDARRQEAGPVSRLVRRLLGFGMKRRQYERGKAFFDAIADERGVAGAAAVWEHPENLPTDDELDAPEAWLARVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYI2</accession>
    <name>A0A1U7EYI2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Probable ribonucleoside-diphosphate reductase beta subunit</fullName>
            <ecNumber evidence="1">1.17.4.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nrdB3</name>
        <name evidence="1" type="ordered locus">NP_4434A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.17.4.1" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323923.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYI2"/>
    <dbReference type="STRING" id="348780.NP_4434A"/>
    <dbReference type="EnsemblBacteria" id="CAI50308">
        <property type="protein sequence ID" value="CAI50308"/>
        <property type="gene ID" value="NP_4434A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702986"/>
    <dbReference type="KEGG" id="nph:NP_4434A"/>
    <dbReference type="eggNOG" id="arCOG03658">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072736_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.620.20">
        <property type="entry name" value="Ribonucleotide Reductase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012348">
        <property type="entry name" value="RNR-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="316" mass="35830" checksum="8C5808A1ED89DAAC" modified="2017-05-10" version="1">MTQIRDDSREMRIDPDSVAGGYFKHAVYNHWDPYEDIEKALIEQDRKRLIDADEFGETEFEDLMRTLALFGAGEEAVTEDLAPLMLVVDDINDQMFVSSQIYEEAKHTQFFDRYWREVIHPVADARGFERRAPTDQRFFVDGYVDLFDRTEAAMERLLEPGEDTPTNRVRAYCHYHLTVESVLAQTGYWGIVSSMSPRGSEALREDGMPHLEGLVKGISFIRSDEGRHVGFGMQKVQSHLAEDGVDESVVRETLQELLPLVAETVSATDEVTDPEPLVEYASEKLTRRIEVITDADASIPDVETLVTLSEDSTAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYJ6</accession>
    <name>A0A1U7EYJ6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">YneT family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4472A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323942.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYJ6"/>
    <dbReference type="STRING" id="348780.NP_4472A"/>
    <dbReference type="EnsemblBacteria" id="CAI50327">
        <property type="protein sequence ID" value="CAI50327"/>
        <property type="gene ID" value="NP_4472A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702072"/>
    <dbReference type="KEGG" id="nph:NP_4472A"/>
    <dbReference type="eggNOG" id="arCOG04227">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112567_0_1_2"/>
    <dbReference type="OrthoDB" id="42776at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33303:SF2">
        <property type="entry name" value="COA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33303">
        <property type="entry name" value="CYTOPLASMIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13380">
        <property type="entry name" value="CoA_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA-binding" evidence="1">
        <location>
            <begin position="12"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="136" mass="14985" checksum="C53D6CB5FEE4CE5E" modified="2017-05-10" version="1">MPVEDDETLESILQLDTIAVVGCSATPQKEAHQVPKFLDERGYEIIPVNPYADEILGREAYDSLSAVEETVDIVDVFRPSEEVSGIVDEAVARDDVAAVWTQLGIRDAEAGERVAAAGKQYVEDKCLKVEYQRLMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYK0</accession>
    <name>A0A1U7EYK0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DUF112 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_4446A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323929.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYK0"/>
    <dbReference type="STRING" id="348780.NP_4446A"/>
    <dbReference type="EnsemblBacteria" id="CAI50314">
        <property type="protein sequence ID" value="CAI50314"/>
        <property type="gene ID" value="NP_4446A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702066"/>
    <dbReference type="KEGG" id="nph:NP_4446A"/>
    <dbReference type="eggNOG" id="arCOG04469">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043916_1_0_2"/>
    <dbReference type="OrthoDB" id="53365at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002823">
        <property type="entry name" value="DUF112_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42204">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42204:SF1">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01970">
        <property type="entry name" value="TctA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="231"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="318"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="352"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="DUF112" evidence="2">
        <location>
            <begin position="14"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="407" mass="39979" checksum="320439267DF41502" modified="2017-05-10" version="1">MEVVLAPRETAVALGFVAAGIALGTASGLVPGLHANNMALLLAGIAPSVPGPPLYVGMAMLSAGVVHTFLEIVPTLALGVPDPAMAVAALPGHRLVLEGRGREALRLSALGSGIAVALAVPLAVPITRLMTAVWPVLRAHLSLVLMAVAAALVLTEPTPGRKLVAAVSFGLSGLLGVATLSLSPEAPLSAGGMLAPLFAGLFGAPVLVDAISGDGIPPQDGPEIAASGRSVTGLAGLGTAAGAVVGYVPAVSSAIAATFALLAVPGRYGARGFIVATSGVNTANTVFALFALIALGSPRTGVLVAVEAASVPLSVPHLLASVGVSALVGVVAVPLVGDRYLAVVGALDPARLSVALLCGLAAVSFLFAGAVGVVAYAAAAVVGLVPARYRARRANLMGVLICPILFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYM3</accession>
    <name>A0A1U7EYM3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DUF92 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">NP_4542A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323977.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYM3"/>
    <dbReference type="STRING" id="348780.NP_4542A"/>
    <dbReference type="EnsemblBacteria" id="CAI50362">
        <property type="protein sequence ID" value="CAI50362"/>
        <property type="gene ID" value="NP_4542A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702094"/>
    <dbReference type="KEGG" id="nph:NP_4542A"/>
    <dbReference type="eggNOG" id="arCOG02245">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036918_2_0_2"/>
    <dbReference type="OrthoDB" id="28948at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002794">
        <property type="entry name" value="DUF92_TMEM19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13353">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13353:SF5">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01940">
        <property type="entry name" value="DUF92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="95"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="123"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="182"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="235"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="289"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="318"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="360"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="388"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="421"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI50362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="440" mass="43942" checksum="0222A92C81A9F081" modified="2017-05-10" version="1">MTRDLRRAVAYLLVGSLVFLAPALDAHYGSSTAAAATAAVFGLVAVAGFKTSDGPLFELFARRRDRAEERLYGLASFALAVAALGVLLVAVELPAVVPVVAVFVLTVGNVGQRLAAGYVASPAVGASAFAAFGTVGGSVAAVAAGWLGAAVASLPLVVFVAASGALLGGLIRSVLYVQDDSLVVLAVALLVWLLLSLGLSPTPERVAVGLAVTVGLGYIAYALDTASVTGMLTGVLLALFAVVLGGYGWFVLLVTFFGLGGLASKYRYDEKLDRGIAQENEGARGGGNVLANSAVALVAVVGYAASGHVGIDATVFKFAFAGAVAAALADTFSSEFGGLFDAPRLITTLERVDPGTDGGVTWQGAVAGATGSGIIAGLGWAFFEFDTIATATVLGAGVLGMVVDSILGATLEGGRLGNQSVNLLATLSAGTAAAVVAVLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYU3</accession>
    <name>A0A1U7EYU3_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">UDP-glucose 6-dehydrogenase</fullName>
            <ecNumber evidence="3 7">1.1.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">aglM1</name>
        <name evidence="13" type="synonym">ugd1</name>
        <name evidence="13" type="ordered locus">NP_4668A</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>UDP-alpha-D-glucose + 2 NAD(+) + H2O = UDP-alpha-D-glucuronate + 2 NADH + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:23596"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="1.1.1.22"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; UDP-alpha-D-glucuronate biosynthesis; UDP-alpha-D-glucuronate from UDP-alpha-D-glucose: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.22" evidence="3 7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324039.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYU3"/>
    <dbReference type="STRING" id="348780.NP_4668A"/>
    <dbReference type="EnsemblBacteria" id="CAI50425">
        <property type="protein sequence ID" value="CAI50425"/>
        <property type="gene ID" value="NP_4668A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703442"/>
    <dbReference type="KEGG" id="nph:NP_4668A"/>
    <dbReference type="eggNOG" id="arCOG00253">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023810_1_1_2"/>
    <dbReference type="OrthoDB" id="59839at2157"/>
    <dbReference type="UniPathway" id="UPA00038">
        <property type="reaction ID" value="UER00491"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003979">
        <property type="term" value="F:UDP-glucose 6-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006065">
        <property type="term" value="P:UDP-glucuronate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.100">
        <property type="entry name" value="Cytochrome c1, transmembrane anchor, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017476">
        <property type="entry name" value="UDP-Glc/GDP-Man"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014027">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036220">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014026">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001732">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028357">
        <property type="entry name" value="UDPglc_DH_bac"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03026">
        <property type="entry name" value="NDP-sugDHase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43750">
        <property type="entry name" value="UDP-GLUCOSE 6-DEHYDROGENASE TUAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43750:SF3">
        <property type="entry name" value="UDP-GLUCOSE 6-DEHYDROGENASE TUAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00984">
        <property type="entry name" value="UDPG_MGDP_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03720">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03721">
        <property type="entry name" value="UDPG_MGDP_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500134">
        <property type="entry name" value="UDPglc_DH_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000124">
        <property type="entry name" value="UDPglc_GDPman_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00984">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52413">
        <property type="entry name" value="UDP-glucose/GDP-mannose dehydrogenase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 10" id="KW-0520">NAD</keyword>
    <keyword evidence="4 7 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDP-glucose/GDP-mannose dehydrogenase C-terminal" evidence="12">
        <location>
            <begin position="345"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="111"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="293"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="181"/>
            <end position="184"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="282"/>
            <end position="286"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="352"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="359"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAI50425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="455" mass="47099" checksum="B9AE4CFA915CF4CC" modified="2017-05-10" version="1">MKLNVVGSGYVGTTLAACLAEMGHNVSAIDIDEATVAALNQGSAPIHEPGLEDLLADHVGDRLQATTSYADAAAADCSFLAVGTPTNDDGSIDVAPLAAAAEMLGAALTEANGESSNSGGNVGSGADGSADAAAEDTHDHLVVVKSTITPPAVATVREALADGASVDSDQLDIEVATNPEFLREGSAVEDFRNPDKLVFGTASEGALETLETLYAPLMASTDAPVVRTDPETAMMIKYANNATLATKISLANDIGNICKAFGLDAYEVMDAVGLDDRIAGAFFESGVGYGGSCFPKDVAALRAAAREAGYEPPLLGATTTVNDRQPDRLLELLASHIDIEGARVAVLGLAFKPGTDDIRNSRAIPVIEGLLDRGATVVGYDPVAAENMRERFPSLTYADSAAEALADATGAVVTTDWDEFAALDSAFDEMATPVVVDGRRIIDRREGITYEGLTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYU8</accession>
    <name>A0A1U7EYU8_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Bifunctional protein GlmU</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">graD1</name>
        <name evidence="7" type="ordered locus">NP_4652A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324031.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYU8"/>
    <dbReference type="STRING" id="348780.NP_4652A"/>
    <dbReference type="EnsemblBacteria" id="CAI50417">
        <property type="protein sequence ID" value="CAI50417"/>
        <property type="gene ID" value="NP_4652A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702770"/>
    <dbReference type="KEGG" id="nph:NP_4652A"/>
    <dbReference type="eggNOG" id="arCOG00666">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_0_1_2"/>
    <dbReference type="OrthoDB" id="15372at2157"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00532"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019134">
        <property type="term" value="F:glucosamine-1-phosphate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003977">
        <property type="term" value="F:UDP-N-acetylglucosamine diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04181">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023915">
        <property type="entry name" value="Bifunctiontional_GlmU_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056729">
        <property type="entry name" value="GMPPB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050486">
        <property type="entry name" value="Mannose-1P_guanyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03992">
        <property type="entry name" value="Arch_glmU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22572">
        <property type="entry name" value="SUGAR-1-PHOSPHATE GUANYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25087">
        <property type="entry name" value="GMPPB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="5">
        <location>
            <begin position="2"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Mannose-1-phosphate guanyltransferase C-terminal" evidence="6">
        <location>
            <begin position="253"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI50417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="384" mass="40200" checksum="A4051977DD22248D" modified="2017-05-10" version="1">MKAAILAAGEGRRLRPLTNRRPKPMLPVGNRPILEHVVAATAAAGLDGIVLVVGYERDRIQTHFGDGDDWDIDIEYAVQKRQLGTGHAVQQVSDRIDGEFLVLNGDRIVNADLIERMAGDVAAPAVAVTRVDQPQRYGIVDTDGDRLRDIDEKPAEPAPSEVINAGVYRFSQSVFETIERTDPDESGERTLPDALAAMAADGDVRAVRYRGTWLDVTQLWDLLATNNDVLDRQGATTRGDIAPSAAIADRVDTASGVAVGPNSTLKRGTTLGANATIGANVVISNAIVMADATIADGAVIRDCIIGENATVGPNTTITGGPAKQVVIDGEVHAEVPLGGVIGDNATLGGNVSVLPGTVLGDGSTVADNATISGTIEPDTEVRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYV8</accession>
    <name>A0A1U7EYV8_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sugar metabolism cluster protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4672A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324041.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYV8"/>
    <dbReference type="STRING" id="348780.NP_4672A"/>
    <dbReference type="EnsemblBacteria" id="CAI50427">
        <property type="protein sequence ID" value="CAI50427"/>
        <property type="gene ID" value="NP_4672A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702138"/>
    <dbReference type="KEGG" id="nph:NP_4672A"/>
    <dbReference type="eggNOG" id="arCOG06398">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_179746_0_0_2"/>
    <dbReference type="OrthoDB" id="294990at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045397">
        <property type="entry name" value="TumE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20126">
        <property type="entry name" value="TumE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="101" mass="11835" checksum="A47C517AE88E30A8" modified="2017-05-10" version="1">MPARKLFEDDDEFPEGDRWEALAWDVSKSDKFPEGLKYSFQYLGPADEEILRYDNANDAHGVGRHHRHSRGEVEGIEFEGLRSHIQNFLEEVETIHEQEYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYW2</accession>
    <name>A0A1U7EYW2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cobalamin import ATP-binding protein BtuD</fullName>
            <ecNumber evidence="8">7.6.2.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Vitamin B12-transporting ATPase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">znuC2</name>
        <name evidence="13" type="synonym">abc20a</name>
        <name evidence="13" type="ordered locus">NP_4730A</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for corrinoid utilization. Probably part of the ABC transporter complex BtuCDF involved in cobalamin (vitamin B12) import. Probably responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>an R-cob(III)alamin(out) + ATP + H2O = an R-cob(III)alamin(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17873"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:140785"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.6.2.8"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">The complex is composed of two ATP-binding proteins (BtuD), two transmembrane proteins (BtuC) and a solute-binding protein (BtuF).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.8" evidence="8"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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</entry>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
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            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
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    <sequence length="413" mass="46269" checksum="6C91C3EA66B107F4" modified="2017-05-10" version="1">MPTKTLEATLAPPTAHKERKLQQLHDLYHEGLHEAFEAGADTQSAVNDIVTPYDLPYQVKDALKNYVPSLDDAQELSDDHPTRMVNRAASFDYSEERTHSYVWEVPQPGRGTNFWVPLRINPEQEQLWADLLDGNVSPGQLQLIQNRTSWKLHVAVEYSVEDADYDTDSDDVTHIGLDIGESSLIMGCALRDGSPTRPLVCDGSRAKHLRKEMHTTLKRLQERDAAEWRIDERFDHYQNALTDIVEKASRQAVEYARGFEKPVLVMEDLTYIRESLDCGKFMNRRLHAWAFARLQDRIEDKARAAGIPVEYVNAAYTSQTCHECGHIGYRDSGGAFHCTNDGCHITTFEGDINGAINIAQRADPWGESVPLKPAGNDSPRDGSACDSTTTHTEQSQSEQMTLGEVGSKPTASN</sequence>
</entry>
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    <accession>A0A1U7EYY4</accession>
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        <submittedName>
            <fullName evidence="7">VKGC domain protein</fullName>
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        <name evidence="7" type="ordered locus">NP_4724A</name>
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        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
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            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
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                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="HTTM-like"/>
    </dbReference>
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        <property type="entry name" value="HTTM_dom"/>
    </dbReference>
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        <property type="entry name" value="Sporulation_signal_mat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39535:SF2">
        <property type="entry name" value="HTTM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39535">
        <property type="entry name" value="SPORULATION-DELAYING PROTEIN SDPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05090">
        <property type="entry name" value="HTTM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HTTM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
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        </location>
    </feature>
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            <end position="239"/>
        </location>
    </feature>
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        <location>
            <begin position="274"/>
            <end position="292"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="22"/>
            <end position="291"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00752"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI50453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="525" mass="59263" checksum="D2583BA25FBECEC5" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYZ2</accession>
    <name>A0A1U7EYZ2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Probable oxidoreductase (Short-chain dehydrogenase family)</fullName>
            <ecNumber evidence="4">1.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_4762A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
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            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
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        <property type="protein sequence ID" value="CAI50472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYZ2"/>
    <dbReference type="STRING" id="348780.NP_4762A"/>
    <dbReference type="EnsemblBacteria" id="CAI50472">
        <property type="protein sequence ID" value="CAI50472"/>
        <property type="gene ID" value="NP_4762A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702169"/>
    <dbReference type="KEGG" id="nph:NP_4762A"/>
    <dbReference type="eggNOG" id="arCOG04552">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_10_2"/>
    <dbReference type="OrthoDB" id="213346at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43669">
        <property type="entry name" value="5-KETO-D-GLUCONATE 5-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43669:SF3">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G03445)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketoreductase" evidence="3">
        <location>
            <begin position="2"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="231" mass="23704" checksum="275332F040248B2E" modified="2017-05-10" version="1">MDTVVITGGSRGVGRAVAADFADAGAHVVVCSRSADDIEAVAADIEADGGEATAVRADVRDEYDAERLLETAARAGDAPGVDCVVANAGVYHGPPGETPLFETSYSSFDDMLRTNARGVFATIREAVPHLSADARVLVPSGNVAREAKAGVGAYAVSKAAAEAIARQFAAELDATVGVVDLGRVDTELTNHGGGRNPESVAQMVRWAAVEADPEAVDGEVVGLAEWKRATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYZ7</accession>
    <name>A0A1U7EYZ7_NATPD</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ordered locus">NP_4786A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
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            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYZ7"/>
    <dbReference type="STRING" id="348780.NP_4786A"/>
    <dbReference type="EnsemblBacteria" id="CAI50484">
        <property type="protein sequence ID" value="CAI50484"/>
        <property type="gene ID" value="NP_4786A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702175"/>
    <dbReference type="KEGG" id="nph:NP_4786A"/>
    <dbReference type="HOGENOM" id="CLU_1567202_0_0_2"/>
    <dbReference type="OrthoDB" id="383870at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="170" mass="18765" checksum="845CC513A8A60E39" modified="2017-05-10" version="1">MNRRSFVAGAFGGAGIVTAGCIDIDSEDWEMVQHCTEEVSVRVDSVGTALEEWLQSPETFDASRFDRLADETETTLDNCESDIRERYEEIEDETIVPDEELEQYDGQEIVSLLQDLLETAHDGQTAAIAVAQADAEPQALDDAEEDIVESVAEDHEEVLDDVEPILAAET</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ12</accession>
    <name>A0A1U7EZ12_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DUF2150 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4844A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ12"/>
    <dbReference type="STRING" id="348780.NP_4844A"/>
    <dbReference type="EnsemblBacteria" id="CAI50513">
        <property type="protein sequence ID" value="CAI50513"/>
        <property type="gene ID" value="NP_4844A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702193"/>
    <dbReference type="KEGG" id="nph:NP_4844A"/>
    <dbReference type="eggNOG" id="arCOG04411">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114819_0_0_2"/>
    <dbReference type="OrthoDB" id="145435at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014518">
        <property type="entry name" value="UCP022079"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09920">
        <property type="entry name" value="DUF2150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF022079">
        <property type="entry name" value="UCP022079"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="193" mass="21141" checksum="4CDECE3434249D2B" modified="2017-05-10" version="1">MSTPPGEYYTAERWNNWLDRLREEEIDPESDDSARLFFNLLDDATIAIAKVITDYEDGEFEDAEAAAAEIDNIRAIVLEEVDIDDEEKLTLVDGIQTSLVCALYAAEEYIVGGPAEEGTIEQYIDAAAAAEAEEEDLDTALAHCVQAGTLVIEGDDIGLDVAEDIEYGFVADWVNGLDSLGRALSDPELVEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ40</accession>
    <name>A0A1U7EZ40_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DUF106 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">NP_4912A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ40"/>
    <dbReference type="STRING" id="348780.NP_4912A"/>
    <dbReference type="EnsemblBacteria" id="CAI50547">
        <property type="protein sequence ID" value="CAI50547"/>
        <property type="gene ID" value="NP_4912A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702199"/>
    <dbReference type="KEGG" id="nph:NP_4912A"/>
    <dbReference type="eggNOG" id="arCOG02673">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_955130_0_0_2"/>
    <dbReference type="OrthoDB" id="84619at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002809">
        <property type="entry name" value="EMC3/TMCO1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038978">
        <property type="entry name" value="MJ0935"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42198">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42198:SF1">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01956">
        <property type="entry name" value="EMC3_TMCO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01415">
        <property type="entry name" value="DUF106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="217"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="269"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="166"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI50547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="298" mass="33513" checksum="C251D4010E0D2AB7" modified="2017-05-10" version="1">MARTASKVQSLVKEDAEMEEALEYVLETADEGSVSWGDVSDELTSGQWGRLIEKGILVDSDGEGFDVDDPDGVREALEDDDLEFPDPPDADSSWTKWDKGAAVGSVAMFAGYMFDSVREPLADALNLLLGPLLDLLPFFAVVMVLALFTGLYSTLLQSNLMDMEVMGKYQGRMKEIQERRKEAKERGDDEALERIQEEQMEAMADNLGMFKEQFRPMVWIMLLTIPVFLWMYAVVGFRGAPLYPEIELVPVVMPIAGEVDWTHGLLGPIQVWIVWYFVCSMCFTQVLRKALNIQTTPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ43</accession>
    <name>A0A1U7EZ43_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">YkgJ family cysteine cluster protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4950A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324178.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ43"/>
    <dbReference type="STRING" id="348780.NP_4950A"/>
    <dbReference type="EnsemblBacteria" id="CAI50566">
        <property type="protein sequence ID" value="CAI50566"/>
        <property type="gene ID" value="NP_4950A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702209"/>
    <dbReference type="KEGG" id="nph:NP_4950A"/>
    <dbReference type="eggNOG" id="arCOG02579">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1507333_0_0_2"/>
    <dbReference type="OrthoDB" id="36424at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005358">
        <property type="entry name" value="Puta_zinc/iron-chelating_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03692">
        <property type="entry name" value="CxxCxxCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="165" mass="18706" checksum="E1DF3629961DE6BB" modified="2017-05-10" version="1">MRVEVYPGKEAVVDFDPSLTFECVDDCTWCCHHGVLLYEQDFLELADRESLAASTASFQGRDFVRRETKPDDHPHTGEDGMACVFLGADGRCQLHAEYDWKPTRCSVFPLEVTVEDGDIHVGIRGEAHDHCDGLDVSERRVVDHLEAFLPELLWELDDPTTKIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ52</accession>
    <name>A0A1U7EZ52_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HTR-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_4932A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324169.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ52"/>
    <dbReference type="STRING" id="348780.NP_4932A"/>
    <dbReference type="EnsemblBacteria" id="CAI50557">
        <property type="protein sequence ID" value="CAI50557"/>
        <property type="gene ID" value="NP_4932A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702204"/>
    <dbReference type="KEGG" id="nph:NP_4932A"/>
    <dbReference type="eggNOG" id="arCOG01172">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053639_1_0_2"/>
    <dbReference type="OrthoDB" id="337234at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637">
        <property type="entry name" value="UPF0273 PROTEIN TM_0370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="278" mass="30303" checksum="E3B1DF8C776FB9CE" modified="2017-05-10" version="1">MDRIPFGIRRLDTTVGGGAPPGSVVLLSGEAGAGAREFMYTSALLNGLARSNPDLFELYYGGVDDRSTPPDRVHYVSFTDGERKLRSEMSLAVDDEIVDEAVGNITFHDLSPEFFRLSPVPREWYAERTQSITELGGSESRKSVPEALGDRLTEHAAGNLVVIDSIGDLISTSTGDLTWGDVPVLLKGLSRAAYNWGGLIIAHVSQEALTDQRHGELVDATDGTLLFEWESGGSARARTMVVKQFRGVLSRIEAEDIVRFETEIGDAGFDISDVRKIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ59</accession>
    <name>A0A1U7EZ59_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">YqeC family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4954A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324180.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ59"/>
    <dbReference type="STRING" id="348780.NP_4954A"/>
    <dbReference type="EnsemblBacteria" id="CAI50568">
        <property type="protein sequence ID" value="CAI50568"/>
        <property type="gene ID" value="NP_4954A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702211"/>
    <dbReference type="KEGG" id="nph:NP_4954A"/>
    <dbReference type="eggNOG" id="arCOG06263">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068045_0_0_2"/>
    <dbReference type="OrthoDB" id="291404at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017587">
        <property type="entry name" value="YqeC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03172">
        <property type="entry name" value="selenium cofactor biosynthesis protein YqeC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19842">
        <property type="entry name" value="YqeC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="240" mass="24936" checksum="28B641FF441D952E" modified="2017-05-10" version="1">MTDIVAALSAADGVTCLVGAGGKKTTLYALAGLLDQPIVTATVRIPIFDREVASVRVTDDPVAELDAAPPYPLGLVAGHDRSDRYRGYGPEIVDAVADAHDGPVLVKADGARTREFKAPNNREPQLPASTATVVPVVSAHAVGKPLDETHVHRPERVAALADIDIGDEITPETVGSVVADEAGGLKDIPDGARAIPLINKVDDAADERTARAIADAIADRADVEAIVLARMATPEVVDVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ69</accession>
    <name>A0A1U7EZ69_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BofA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4976A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324190.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ69"/>
    <dbReference type="EnsemblBacteria" id="CAI50579">
        <property type="protein sequence ID" value="CAI50579"/>
        <property type="gene ID" value="NP_4976A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702218"/>
    <dbReference type="KEGG" id="nph:NP_4976A"/>
    <dbReference type="eggNOG" id="arCOG05132">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_172280_0_0_2"/>
    <dbReference type="OrthoDB" id="205985at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010001">
        <property type="entry name" value="BofA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07441">
        <property type="entry name" value="BofA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="95" mass="9584" checksum="FFAA37980E355813" modified="2017-05-10" version="1">MVTTLELTLLVIALALLFGAYTVIKAVKPFIVNAVVGVIVLFLASALGLGVEITPVAVLICAVGGIPGAILVMILAYADIAFAGMVMPFGAALLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ89</accession>
    <name>A0A1U7EZ89_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">CopG domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4988A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ89"/>
    <dbReference type="EnsemblBacteria" id="CAI50585">
        <property type="protein sequence ID" value="CAI50585"/>
        <property type="gene ID" value="NP_4988A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_4988A"/>
    <dbReference type="eggNOG" id="arCOG08173">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3075448_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="52" mass="6024" checksum="C2BE0CD203681366" modified="2017-05-10" version="1">MPIGNTRHYMPEYTLECDDQQAQQVQRLAVRHGLTEREVLEQLVAVGLEELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZA0</accession>
    <name>A0A1U7EZA0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5050A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324226.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZA0"/>
    <dbReference type="STRING" id="348780.NP_5050A"/>
    <dbReference type="EnsemblBacteria" id="CAI50616">
        <property type="protein sequence ID" value="CAI50616"/>
        <property type="gene ID" value="NP_5050A"/>
    </dbReference>
    <dbReference type="GeneID" id="41345114"/>
    <dbReference type="KEGG" id="nph:NP_5050A"/>
    <dbReference type="eggNOG" id="arCOG15216">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_208211_1_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058858">
        <property type="entry name" value="HacaP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26396">
        <property type="entry name" value="HacaP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="53" mass="6586" checksum="4CA2372D2B16B338" modified="2017-05-10" version="1">MPDTKRGRERKGMKKQEQRRRREIERAMEARDHEIDLDAVYEDEEELEIELDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZA3</accession>
    <name>A0A1U7EZA3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5094A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324248.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZA3"/>
    <dbReference type="EnsemblBacteria" id="CAI50638">
        <property type="protein sequence ID" value="CAI50638"/>
        <property type="gene ID" value="NP_5094A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702252"/>
    <dbReference type="KEGG" id="nph:NP_5094A"/>
    <dbReference type="eggNOG" id="arCOG04731">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1544167_0_0_2"/>
    <dbReference type="OrthoDB" id="239492at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057180">
        <property type="entry name" value="DUF7858"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25257">
        <property type="entry name" value="DUF7858"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="165" mass="16888" checksum="8A5A2AEAE764327E" modified="2017-05-10" version="1">MGLADIASGLEVTAEQQERGVATTDATETPLAERLAPFAERLPCSATAAATLVEAYADGASVGRAAAVAELPKTTAAKALYLLGEPIEPVSPTARRVLEDWLAGDLSRTEAKRLAGVGDRAFALGTYVATHEPIAAAEAAVADELSVSSPADPLSDTRSDLGDLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZB3</accession>
    <name>A0A1U7EZB3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable S-adenosylmethionine-dependent methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5122A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324262.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZB3"/>
    <dbReference type="STRING" id="348780.NP_5122A"/>
    <dbReference type="EnsemblBacteria" id="CAI50652">
        <property type="protein sequence ID" value="CAI50652"/>
        <property type="gene ID" value="NP_5122A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702264"/>
    <dbReference type="KEGG" id="nph:NP_5122A"/>
    <dbReference type="eggNOG" id="arCOG01783">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1048140_0_0_2"/>
    <dbReference type="OrthoDB" id="142890at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF24">
        <property type="entry name" value="2-METHOXY-6-POLYPRENYL-1,4-BENZOQUINOL METHYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="1">
        <location>
            <begin position="36"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="261" mass="28214" checksum="36874AF39B1012E2" modified="2017-05-10" version="1">MREFSAEYLRTTRRGMWADSRAALSPLDLGGCERILDVGCGEGALTRVLDEESPGTVVGCDRDPGLLVELSGPAVQGDAYQLPFADGSFDLVVCQALLVNLPEPERAIDEFARVAADRVACIEPDNSAVAVESTVDAESALAARARQRYLAGVDTDVALGADAADLFREAGLSNVRTRRYDQTLVVEPPYSEAAVRAAGRKASGEGLRKRRETMAGTEEALDALRSDWREMGREAVQQIQAGKYRRKETVPFYVTVGEVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZC8</accession>
    <name>A0A1U7EZC8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Lactate 2-monooxygenase</fullName>
            <ecNumber evidence="7">1.13.12.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">NP_5124A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.13.12.4" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324263.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZC8"/>
    <dbReference type="STRING" id="348780.NP_5124A"/>
    <dbReference type="EnsemblBacteria" id="CAI50653">
        <property type="protein sequence ID" value="CAI50653"/>
        <property type="gene ID" value="NP_5124A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702859"/>
    <dbReference type="KEGG" id="nph:NP_5124A"/>
    <dbReference type="eggNOG" id="arCOG00613">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020639_0_0_2"/>
    <dbReference type="OrthoDB" id="56968at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050040">
        <property type="term" value="F:lactate 2-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03332">
        <property type="entry name" value="LMO_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000029">
        <property type="entry name" value="L-lactate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012133">
        <property type="entry name" value="Alpha-hydoxy_acid_DH_FMN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037396">
        <property type="entry name" value="FMN_HAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037350">
        <property type="entry name" value="LMO_FMN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578:SF143">
        <property type="entry name" value="FMN-DEPENDENT ALPHA-HYDROXY ACID DEHYDROGENASE PB1A11.03"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578">
        <property type="entry name" value="S -2-HYDROXY-ACID OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000138">
        <property type="entry name" value="Al-hdrx_acd_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51349">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN hydroxy acid dehydrogenase" evidence="6">
        <location>
            <begin position="22"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI50653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="396" mass="43702" checksum="2280E64145E16DA2" modified="2017-05-10" version="1">MSEQFGNRKVNRVYREGMHEGVVPEFPASYEDLREQAHEVMDETARAYIHGGAGAEETFRTEQDFSEWRIVPRMLRGVEDRDLSTEVLGQTVDYPAMVTPLGVQTLVDEEGELATARACDELHVPFILSSLSSTPMEEVAEALGDTPKWFQFYWSADEDIARSFLTRAEEAGYDAIVVTVDAPTLGWRERLIDRGYYPFLEGEGVANYFSDPEFRSQLEAPPEEEPQAAVDHFLDIFGDASLTWDDLEFVFEHTDLPVLIKGVLHPEDAKLAVEHGADGVGVSTHGGRQVDGSITALEALPDIVDAVGDDVTVTFDSGIRRGADIYKALALGADACLIGRPFIYGLALGGQDGVEHVLENLIADFDLTMGLAGRDAATDLDRETLRHESHLPGRRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILU6</accession>
    <name>Q3ILU6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3292A</name>
        <name evidence="3" type="ordered locus">NP_7016A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>PL23</name>
    </geneLocation>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
            <strain evidence="3">Gabara</strain>
            <plasmid evidence="3">PL23</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CR936259">
        <property type="protein sequence ID" value="CAI50924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323358.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILU6"/>
    <dbReference type="STRING" id="348780.NP_3292A"/>
    <dbReference type="EnsemblBacteria" id="CAI49737">
        <property type="protein sequence ID" value="CAI49737"/>
        <property type="gene ID" value="NP_3292A"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI50924">
        <property type="protein sequence ID" value="CAI50924"/>
        <property type="gene ID" value="NP_7016A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703468"/>
    <dbReference type="KEGG" id="nph:NP_3292A"/>
    <dbReference type="KEGG" id="nph:NP_7016A"/>
    <dbReference type="HOGENOM" id="CLU_2217130_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="108" mass="10832" checksum="F7AFD986CC329305" modified="2005-11-08" version="1">MSASYAHRGADAVEHASDATGVSEARIMGTVRVIGGAMIGIAVLALVLTEVFSLEQFAQDADGNYEGPFGGVLDSLTSTGPAALGLLVIGLLVAAANRVMGFFGGSGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILV0</accession>
    <name>Q3ILV0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3284A</name>
        <name evidence="2" type="ordered locus">NP_7008A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>PL23</name>
    </geneLocation>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
            <strain evidence="2">Gabara</strain>
            <plasmid evidence="2">PL23</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CR936259">
        <property type="protein sequence ID" value="CAI50920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323354.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILV0"/>
    <dbReference type="STRING" id="348780.NP_3284A"/>
    <dbReference type="EnsemblBacteria" id="CAI49733">
        <property type="protein sequence ID" value="CAI49733"/>
        <property type="gene ID" value="NP_3284A"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI50920">
        <property type="protein sequence ID" value="CAI50920"/>
        <property type="gene ID" value="NP_7008A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703464"/>
    <dbReference type="KEGG" id="nph:NP_3284A"/>
    <dbReference type="KEGG" id="nph:NP_7008A"/>
    <dbReference type="eggNOG" id="arCOG07960">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_920148_0_0_2"/>
    <dbReference type="OrthoDB" id="204371at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="302" mass="34560" checksum="071156CB0D89E0B6" modified="2005-11-08" version="1">MAVSVDHGHEPHDLDGVLDADLAAELRKREADPAPTEAAESLFGAFNGPFFRELAQRKAENRDAKCLVTAKDGQTGIGKSNCCDFLGYVSDTSAAGFSEHKTTIDPFEFLDFYGHLPPGSATIMEEGEQFDARRSNSNKNIDAAERWQMARVREIIAFVNLPSPEEIDSRFERLADYWINIERRGFARVYKKRIHPTKRVLYYETLQTFEWPNMDDSESFKYMDALKDDRLDDGDHGSNWVRQSEVNEMVERARKEAREEERDKWIASVYRDTEMTGKDVARLEACEVSRGRVSQIASEVRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM21</accession>
    <name>Q3IM21_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">RelE family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_6130A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324446.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM21"/>
    <dbReference type="EnsemblBacteria" id="CAI50845">
        <property type="protein sequence ID" value="CAI50845"/>
        <property type="gene ID" value="NP_6130A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694617"/>
    <dbReference type="KEGG" id="nph:NP_6130A"/>
    <dbReference type="HOGENOM" id="CLU_173005_0_0_2"/>
    <dbReference type="OrthoDB" id="159002at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2310.20">
        <property type="entry name" value="RelE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035093">
        <property type="entry name" value="RelE/ParE_toxin_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143011">
        <property type="entry name" value="RelE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="96" mass="11257" checksum="20FBA1BB1E081FBB" modified="2005-11-08" version="1">MPASDDEWDWRLTDPAEREYEKLQPHEQERIVSKLDEIVTDQWRDPSDYLEPLTGAPHSKLRIGAFRLGCKAVQDDQILWVYTIEKRPGAYTPDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM49</accession>
    <name>Q3IM49_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ISH4-type transposase ISNph2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_6068A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324418.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM49"/>
    <dbReference type="EnsemblBacteria" id="CAI50814">
        <property type="protein sequence ID" value="CAI50814"/>
        <property type="gene ID" value="NP_6068A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694699"/>
    <dbReference type="KEGG" id="nph:NP_6068A"/>
    <dbReference type="HOGENOM" id="CLU_044348_1_2_2"/>
    <dbReference type="OrthoDB" id="86086at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024445">
        <property type="entry name" value="Tnp_ISXO2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051354">
        <property type="entry name" value="Transposase_27_IS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024442">
        <property type="entry name" value="Transposase_Zn_ribbon"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033547">
        <property type="entry name" value="transpos_IS1595"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33293">
        <property type="entry name" value="INSERTION ELEMENT IS1 1 PROTEIN INSB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33293:SF1">
        <property type="entry name" value="INSERTION ELEMENT IS1 1 PROTEIN INSB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12762">
        <property type="entry name" value="DDE_Tnp_IS1595"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12760">
        <property type="entry name" value="Zn_ribbon_IS1595"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01126">
        <property type="entry name" value="DDE_Tnp_IS1595"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ISXO2-like transposase" evidence="2">
        <location>
            <begin position="120"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="294" mass="33963" checksum="91A22AF2CDDCBD1F" modified="2005-11-08" version="1">MFPFELLSSEASAANLLEQVRWREGLCCPRCRSESVIKHGSYREYQRYLCKDCDRTFNDKTGTIFAHAKIGLDKLLFAFYSLLRFNTSIRQLDAEIDVSYRSLRRRVEQFARTLDAPAINLVGPVEIDEFYVSAGKKGRKRDRESRSRALSKRGRGTYEEDKPPVFTLVDRGTGQRYVVPAKSADEATVRLLLENHEKESLTVYTDGFRAYDPLDDDEDFHRESVIHGDGEYVDGDAHVNTCESHASLARRWLSPHRGVSKDKLTAYLRPFQLRRRILRKPGREALKQIIREVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM61</accession>
    <name>Q3IM61_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_6038A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324406.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM61"/>
    <dbReference type="EnsemblBacteria" id="CAI50799">
        <property type="protein sequence ID" value="CAI50799"/>
        <property type="gene ID" value="NP_6038A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694592"/>
    <dbReference type="KEGG" id="nph:NP_6038A"/>
    <dbReference type="HOGENOM" id="CLU_1737409_0_0_2"/>
    <dbReference type="OrthoDB" id="200315at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="165" mass="18307" checksum="9DFAF6A7ADB1A4AE" modified="2005-11-08" version="1">MLDGYVDIESRTSELMLASGVLWAAVLALGLAGRWFVALLLSVFLFHPWFVIGASSGGKISTKLLVYPLGVWTGLQLSAFILVEYYANAFSGAPDFLITGMHPSFAAAYWLYWVGGFMTVTLAYGLYFRTYFLPEGEWDRFLEEVEQVKAETNTTGQSESAEVDD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-09-05" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMF1</accession>
    <name>RL37A_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein eL43</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">50S ribosomal protein L37Ae</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Ribosomal protein L43e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl37ae</name>
        <name type="ordered locus">NP_5232A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL43 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324317.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMF1"/>
    <dbReference type="SMR" id="Q3IMF1"/>
    <dbReference type="STRING" id="348780.NP_5232A"/>
    <dbReference type="EnsemblBacteria" id="CAI50707">
        <property type="protein sequence ID" value="CAI50707"/>
        <property type="gene ID" value="NP_5232A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703142"/>
    <dbReference type="KEGG" id="nph:NP_5232A"/>
    <dbReference type="eggNOG" id="arCOG04208">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141199_2_0_2"/>
    <dbReference type="OrthoDB" id="372011at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00327">
        <property type="entry name" value="Ribosomal_eL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_eL37/eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002674">
        <property type="entry name" value="Ribosomal_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050522">
        <property type="entry name" value="Ribosomal_protein_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L37A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01780">
        <property type="entry name" value="Ribosomal_L37ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000418992" description="Large ribosomal subunit protein eL43">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="59"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="86" mass="9179" checksum="6BF8AB5FF34DACC5" modified="2005-11-08" version="1">MANNDTTGSAGRFGARYGRVARRRVAEIEADTESATVDGDSVKRVGTGIWVNEETGEKFTGGAYRPQTPAGETVERSIRTAVGDDE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMN0</accession>
    <name>RS24_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein eS24</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">30S ribosomal protein S24e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps24e</name>
        <name type="ordered locus">NP_5074A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS24 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324238.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMN0"/>
    <dbReference type="SMR" id="Q3IMN0"/>
    <dbReference type="STRING" id="348780.NP_5074A"/>
    <dbReference type="EnsemblBacteria" id="CAI50628">
        <property type="protein sequence ID" value="CAI50628"/>
        <property type="gene ID" value="NP_5074A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703176"/>
    <dbReference type="KEGG" id="nph:NP_5074A"/>
    <dbReference type="eggNOG" id="arCOG04182">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107248_3_1_2"/>
    <dbReference type="OrthoDB" id="27533at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00545">
        <property type="entry name" value="Ribosomal_eS24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001976">
        <property type="entry name" value="Ribosomal_eS24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10496">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01282">
        <property type="entry name" value="Ribosomal_S24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_1000017748" description="Small ribosomal subunit protein eS24">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="93"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="105" mass="11838" checksum="3F7CA1DB35B8B5E6" modified="2005-11-08" version="1">MDVDIIDEDENPMLHRTDVRFQLTHEEATPSRLSVRDSLAAKLNKDASEVVVRKLDTKYGMRKTVGHAKVYDSADDAKAVEQDHALERNKIEADEEADEEAAEEA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-04-08" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q3INR7</accession>
    <name>COXX2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName>Protoheme IX farnesyltransferase 2</fullName>
            <ecNumber>2.5.1.141</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Heme B farnesyltransferase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Heme O synthase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">ctaB2</name>
        <name type="ordered locus">NP_4288A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>heme b + (2E,6E)-farnesyl diphosphate + H2O = Fe(II)-heme o + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:28070"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="ChEBI" id="CHEBI:60530"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.141"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Porphyrin-containing compound metabolism; heme O biosynthesis; heme O from protoheme: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Carbon 2 of the heme B porphyrin ring is defined according to the Fischer nomenclature.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the UbiA prenyltransferase family. Protoheme IX farnesyltransferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.141"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323851.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3INR7"/>
    <dbReference type="SMR" id="Q3INR7"/>
    <dbReference type="STRING" id="348780.NP_4288A"/>
    <dbReference type="EnsemblBacteria" id="CAI50235">
        <property type="protein sequence ID" value="CAI50235"/>
        <property type="gene ID" value="NP_4288A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702643"/>
    <dbReference type="KEGG" id="nph:NP_4288A"/>
    <dbReference type="eggNOG" id="arCOG00479">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030009_1_1_2"/>
    <dbReference type="OrthoDB" id="131615at2157"/>
    <dbReference type="UniPathway" id="UPA00834">
        <property type="reaction ID" value="UER00712"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008495">
        <property type="term" value="F:protoheme IX farnesyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048034">
        <property type="term" value="P:heme O biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13957">
        <property type="entry name" value="PT_UbiA_Cox10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00154">
        <property type="entry name" value="CyoE_CtaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006369">
        <property type="entry name" value="Protohaem_IX_farnesylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01473">
        <property type="entry name" value="cyoE_ctaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003349">
        <property type="entry name" value="PRK04375.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43448">
        <property type="entry name" value="PROTOHEME IX FARNESYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43448:SF2">
        <property type="entry name" value="PROTOHEME IX FARNESYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0350">Heme biosynthesis</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000327205" description="Protoheme IX farnesyltransferase 2">
        <location>
            <begin position="1"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="4"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="257"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="282"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="308"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="357"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="378"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="413"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Unknown">
        <location>
            <begin position="1"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Protoheme IX prenyltransferase">
        <location>
            <begin position="165"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="433" mass="45372" checksum="F008932238C33F64" modified="2005-11-08" version="1">MQRFTGLVTATTLATYLLVVLGVATELTGGVSPAAVAHYVTAGAVWLLLVAAAALAWRDSRLPRVKWGVTAAAVAYPAQAAVGMAVLASGGPGQLHLFGGVGVFALLLITLTWHLDREVEPRERAAATAFNREGDGDDSVLLYRLPDGLRRYVELTKPRLMWLLCLLALSGMALATVTGAALDGVTIAATLFGGVLAVGAAGTFNHVYERDRDRRMNRTADRPVATDAVGVGRATAFGVGLLVVSMAVLVWLVNPLAAALTAVAVVYYAVVYTVVLKPTTTWNTVIGGGAGALPAVIGWAAVAGSIGLPALLLAAVVFCWTPAHFYNLAIAYRDDYARGDYPMLPVVAGVAATRRRILYWLGATLLVAGALGAVAGFGPVYALTSAVVGFGFLWTVVVQFRTESDRDAYRSFHASNAYLGALLVAILVETMVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IQF2</accession>
    <name>Q3IQF2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Type IV pilus biogenesis complex ATPase subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">pilB1</name>
        <name evidence="5" type="ordered locus">NP_3106A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GSP E family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323266.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IQF2"/>
    <dbReference type="STRING" id="348780.NP_3106A"/>
    <dbReference type="EnsemblBacteria" id="CAI49644">
        <property type="protein sequence ID" value="CAI49644"/>
        <property type="gene ID" value="NP_3106A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701654"/>
    <dbReference type="KEGG" id="nph:NP_3106A"/>
    <dbReference type="eggNOG" id="arCOG01817">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005379_2_0_2"/>
    <dbReference type="OrthoDB" id="33500at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01130">
        <property type="entry name" value="VirB11-like_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056571">
        <property type="entry name" value="PilB3-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056570">
        <property type="entry name" value="PilB3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050921">
        <property type="entry name" value="T4SS_GSP_E_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30486">
        <property type="entry name" value="TWITCHING MOTILITY PROTEIN PILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30486:SF6">
        <property type="entry name" value="TYPE IV PILUS RETRACTATION ATPASE PILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23989">
        <property type="entry name" value="PilB3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23990">
        <property type="entry name" value="PilB3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PilB3-like N-terminal" evidence="4">
        <location>
            <begin position="12"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Bacterial type II secretion system protein E" evidence="2">
        <location>
            <begin position="240"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="PilB3-like C-terminal" evidence="3">
        <location>
            <begin position="459"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="546" mass="63316" checksum="2152BB7050EE27DB" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IQI4</accession>
    <name>Q3IQI4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type transport system permease protein (Probable substrate tungstate)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tupB</name>
        <name evidence="7" type="synonym">abc22p</name>
        <name evidence="7" type="ordered locus">NP_3042A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323234.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IQI4"/>
    <dbReference type="STRING" id="348780.NP_3042A"/>
    <dbReference type="EnsemblBacteria" id="CAI49612">
        <property type="protein sequence ID" value="CAI49612"/>
        <property type="gene ID" value="NP_3042A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702433"/>
    <dbReference type="KEGG" id="nph:NP_3042A"/>
    <dbReference type="eggNOG" id="arCOG00166">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_14_2_2"/>
    <dbReference type="OrthoDB" id="94632at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049783">
        <property type="entry name" value="ABC_perm_TupB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038017">
        <property type="entry name" value="ABC_perm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43632">
        <property type="entry name" value="PERMEASE COMPONENT OF TUNGSTATE ABC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43632:SF1">
        <property type="entry name" value="PERMEASE COMPONENT OF TUNGSTATE ABC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="30"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="67"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="32"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="241" mass="24239" checksum="E987CF62EBC03182" modified="2005-11-08" version="1">MSLGGLSLLAVADALPVVGDLPFERHYITSIIYVSLYVSLTAVVLSTLISIPIALGVGFADFPGKGVVVAVINTGMGFPSVVVGLLVLFAVSNQGPLGGLGLVYTKEAMILSQIVLATPVITGVSLAAVTSVGDGVRDAAYAMGATKMDIALVTVKEARYGIATGVLAGLGRAISEVGSVLIVGGNIAGADGTAITRTLTTAIQLEARQGRFEAALVLGGILVALVLVINGLVLRLGGGRI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-07-25" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IR98</accession>
    <name>ATGT_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA-guanine(15) transglycosylase</fullName>
            <ecNumber evidence="1">2.4.2.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">7-cyano-7-deazaguanine tRNA-ribosyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Archaeal tRNA-guanine transglycosylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tgtA</name>
        <name type="ordered locus">NP_2508A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Exchanges the guanine residue with 7-cyano-7-deazaguanine (preQ0) at position 15 in the dihydrouridine loop (D-loop) of archaeal tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>guanosine(15) in tRNA + 7-cyano-7-carbaguanine = 7-cyano-7-carbaguanosine(15) in tRNA + guanine</text>
            <dbReference type="Rhea" id="RHEA:43164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10371"/>
            <dbReference type="Rhea" id="RHEA-COMP:10372"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:45075"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:82850"/>
            <dbReference type="EC" id="2.4.2.48"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; archaeosine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeosine tRNA-ribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.48" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322971.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IR98"/>
    <dbReference type="SMR" id="Q3IR98"/>
    <dbReference type="STRING" id="348780.NP_2508A"/>
    <dbReference type="EnsemblBacteria" id="CAI49345">
        <property type="protein sequence ID" value="CAI49345"/>
        <property type="gene ID" value="NP_2508A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703241"/>
    <dbReference type="KEGG" id="nph:NP_2508A"/>
    <dbReference type="eggNOG" id="arCOG00989">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030083_0_0_2"/>
    <dbReference type="OrthoDB" id="6871at2157"/>
    <dbReference type="UniPathway" id="UPA00393"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002099">
        <property type="term" value="P:tRNA wobble guanine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.105">
        <property type="entry name" value="Queuine tRNA-ribosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01634">
        <property type="entry name" value="TgtA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050076">
        <property type="entry name" value="ArchSynthase1/Queuine_TRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036511">
        <property type="entry name" value="TGT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004804">
        <property type="entry name" value="TgtA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002616">
        <property type="entry name" value="tRNA_ribo_trans-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00432">
        <property type="entry name" value="arcsn_tRNA_tgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00449">
        <property type="entry name" value="tgt_general"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46499">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46499:SF1">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01702">
        <property type="entry name" value="TGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88802">
        <property type="entry name" value="Pre-PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51713">
        <property type="entry name" value="tRNA-guanine transglycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000247879" description="tRNA-guanine(15) transglycosylase">
        <location>
            <begin position="1"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="485"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="489"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="503"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="520" mass="57073" checksum="0319B50A8442C30D" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IRM3</accession>
    <name>HIS7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Imidazoleglycerol-phosphate dehydratase</fullName>
            <shortName evidence="1">IGPD</shortName>
            <ecNumber evidence="1">4.2.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisB</name>
        <name type="ordered locus">NP_2258A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:11040"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="EC" id="4.2.1.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 6/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the imidazoleglycerol-phosphate dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.19" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322847.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IRM3"/>
    <dbReference type="SMR" id="Q3IRM3"/>
    <dbReference type="STRING" id="348780.NP_2258A"/>
    <dbReference type="EnsemblBacteria" id="CAI49220">
        <property type="protein sequence ID" value="CAI49220"/>
        <property type="gene ID" value="NP_2258A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702815"/>
    <dbReference type="KEGG" id="nph:NP_2258A"/>
    <dbReference type="eggNOG" id="arCOG04398">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044308_3_0_2"/>
    <dbReference type="OrthoDB" id="103579at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00011"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004424">
        <property type="term" value="F:imidazoleglycerol-phosphate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07914">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.40:FF:000001">
        <property type="entry name" value="Imidazoleglycerol-phosphate dehydratase HisB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.40:FF:000003">
        <property type="entry name" value="Imidazoleglycerol-phosphate dehydratase HisB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.40">
        <property type="entry name" value="Imidazole glycerol phosphate dehydratase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00076">
        <property type="entry name" value="HisB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038494">
        <property type="entry name" value="IGPD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000807">
        <property type="entry name" value="ImidazoleglycerolP_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020565">
        <property type="entry name" value="ImidazoleglycerP_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002111">
        <property type="entry name" value="PRK00951.2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002114">
        <property type="entry name" value="PRK00951.2-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002116">
        <property type="entry name" value="PRK00951.2-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23133:SF2">
        <property type="entry name" value="IMIDAZOLEGLYCEROL-PHOSPHATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23133">
        <property type="entry name" value="IMIDAZOLEGLYCEROL-PHOSPHATE DEHYDRATASE HIS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00475">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00954">
        <property type="entry name" value="IGP_DEHYDRATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00955">
        <property type="entry name" value="IGP_DEHYDRATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000010308" description="Imidazoleglycerol-phosphate dehydratase">
        <location>
            <begin position="1"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00076"/>
        </source>
    </evidence>
    <sequence length="195" mass="21242" checksum="2A98701572D1FBE3" modified="2005-11-08" version="1">MTDRTAAATRETAETEIEVTLDLDGDGESTVDTGIGFFDHMLEAFAKHGLFDLTVRCDGDLDIDDHHTVEDIGIVLGECLADALGDKRGIVRYADRAVPLDEAVADVVVDVSGRPRFYFDGAFSQPYIGEFTSDMARHFAESLALNAGLTLHLSIEGENAHHEVEALFKALARSLDDATRVDDRRSDTPSTKGEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IRN3</accession>
    <name>DHQS_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-dehydroquinate synthase</fullName>
            <shortName evidence="1">DHQ synthase</shortName>
            <ecNumber evidence="1">1.4.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">3-dehydroquinate synthase II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aroB'</name>
        <name type="ordered locus">NP_2238A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the oxidative deamination and cyclization of 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonic acid (ADH) to yield 3-dehydroquinate (DHQ), which is fed into the canonical shikimic pathway of aromatic amino acid biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-amino-2,3,7-trideoxy-D-lyxo-hept-6-ulosonate + NAD(+) + H2O = 3-dehydroquinate + NH4(+) + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25956"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58859"/>
            <dbReference type="EC" id="1.4.1.24"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal-type DHQ synthase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.24" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322837.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IRN3"/>
    <dbReference type="SMR" id="Q3IRN3"/>
    <dbReference type="STRING" id="348780.NP_2238A"/>
    <dbReference type="EnsemblBacteria" id="CAI49210">
        <property type="protein sequence ID" value="CAI49210"/>
        <property type="gene ID" value="NP_2238A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701388"/>
    <dbReference type="KEGG" id="nph:NP_2238A"/>
    <dbReference type="eggNOG" id="arCOG04353">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056379_0_0_2"/>
    <dbReference type="OrthoDB" id="10265at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003856">
        <property type="term" value="F:3-dehydroquinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102042">
        <property type="term" value="F:dehydroquinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01244">
        <property type="entry name" value="Arch_DHQ_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002812">
        <property type="entry name" value="DHQS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030960">
        <property type="entry name" value="DHQS/DOIS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056179">
        <property type="entry name" value="DHQS_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002623">
        <property type="entry name" value="PRK02290.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33563">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33563:SF1">
        <property type="entry name" value="3-DEHYDROQUINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01959">
        <property type="entry name" value="DHQS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26558">
        <property type="entry name" value="DHQS_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006655">
        <property type="entry name" value="DHQ_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000067070" description="3-dehydroquinate synthase">
        <location>
            <begin position="1"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01244"/>
        </source>
    </evidence>
    <sequence length="388" mass="42340" checksum="E42748E767C31481" modified="2005-11-08" version="1">MTRSVWLKADDAVGDWEARKRRITAGLEAGVDWVLVDERDVSRVRELGQVNVAAFAGDDVHVMDAEEQPDAEPDAVIVGKEGEGDGTVDLPSDFSGSADLTTLRRAEAPAGAYVRILDQDYESFAETAAVDADYTIVIGDDWQIIPLENLIARIGDETDLIAGVQTAEEAETAFETLELGADAVLLDTDNPDEIRATVEARDATERETLDLQRATVTEIEETGSADRVCVDTASMLEHDEGMLVGSMSRGLFFVHAETADSPYVASRPFRVNAGAVHAYVRTPDGGTKYLAELSSGDEVQVVDTDGNTREAIVGRAKIEKRPMFRIEAELENGDRIETLLQNAETIKVATDEGRRSVTELEAGDELLIYYEDVARHFGEAVEESIIEK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-02-21" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IRZ1</accession>
    <name>UREE_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Urease accessory protein UreE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ureE</name>
        <name type="ordered locus">NP_2018A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in urease metallocenter assembly. Binds nickel. Probably functions as a nickel donor during metallocenter assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UreE family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322729.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IRZ1"/>
    <dbReference type="SMR" id="Q3IRZ1"/>
    <dbReference type="STRING" id="348780.NP_2018A"/>
    <dbReference type="EnsemblBacteria" id="CAI49100">
        <property type="protein sequence ID" value="CAI49100"/>
        <property type="gene ID" value="NP_2018A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703452"/>
    <dbReference type="KEGG" id="nph:NP_2018A"/>
    <dbReference type="eggNOG" id="arCOG06188">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095954_0_0_2"/>
    <dbReference type="OrthoDB" id="241983at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00822">
        <property type="entry name" value="UreE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012406">
        <property type="entry name" value="UreE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004029">
        <property type="entry name" value="UreE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036118">
        <property type="entry name" value="UreE_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009752">
        <property type="entry name" value="PRK13261.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02814">
        <property type="entry name" value="UreE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00988">
        <property type="entry name" value="UreE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69287">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0533">Nickel</keyword>
    <keyword id="KW-0996">Nickel insertion</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000223462" description="Urease accessory protein UreE">
        <location>
            <begin position="1"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="163"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="168"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="214" mass="23560" checksum="3B6577E2CCA6AA4A" modified="2005-11-08" version="1">MRHVDGVVGNRYDDPDLDEQLHAHEDAGRLERVVLEAGQRKRSRLRVETDAGTDLGIVVDQPELRAGDVLFLDDDAAAVVEFESRTAFVVDLPSPGPETVAAAVELGHRVGNQHWDIAIEGGTVYVPVEADRRIIEDVLGEHIPDSGTTRYASVDASLFIGENAEPSGVDHSHEATDSGHGYGEDHDHDHSHDHNHDHDHNHDHDHSHSHDSHE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-12-06" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IT24</accession>
    <name>RLME_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosomal RNA large subunit methyltransferase E</fullName>
            <ecNumber evidence="1">2.1.1.166</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">23S rRNA Um2552 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">rRNA (uridine-2'-O-)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rlmE</name>
        <name evidence="1" type="synonym">rrmJ</name>
        <name type="ordered locus">NP_1238A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>uridine(2552) in 23S rRNA + S-adenosyl-L-methionine = 2'-O-methyluridine(2552) in 23S rRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42720"/>
            <dbReference type="Rhea" id="RHEA-COMP:10202"/>
            <dbReference type="Rhea" id="RHEA-COMP:10203"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74478"/>
            <dbReference type="EC" id="2.1.1.166"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.166" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322346.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IT24"/>
    <dbReference type="SMR" id="Q3IT24"/>
    <dbReference type="STRING" id="348780.NP_1238A"/>
    <dbReference type="EnsemblBacteria" id="CAI48710">
        <property type="protein sequence ID" value="CAI48710"/>
        <property type="gene ID" value="NP_1238A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701091"/>
    <dbReference type="KEGG" id="nph:NP_1238A"/>
    <dbReference type="eggNOG" id="arCOG00079">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009422_4_4_2"/>
    <dbReference type="OrthoDB" id="26307at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008650">
        <property type="term" value="F:rRNA (uridine-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01547">
        <property type="entry name" value="RNA_methyltr_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050082">
        <property type="entry name" value="RNA_methyltr_RlmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015507">
        <property type="entry name" value="rRNA-MeTfrase_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920:SF13">
        <property type="entry name" value="PRE-RRNA 2'-O-RIBOSE RNA METHYLTRANSFERASE FTSJ3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920">
        <property type="entry name" value="RIBOSOMAL RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000155571" description="Ribosomal RNA large subunit methyltransferase E">
        <location>
            <begin position="1"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="1">
        <location>
            <begin position="194"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01547"/>
        </source>
    </evidence>
    <sequence length="252" mass="27393" checksum="2434DDD08EC72B7D" modified="2005-11-08" version="1">MTRKDDYYNRAKQQGYRSRAAYKLKQLDEAADLINEGDTVVDLGAAPGGWLQVANELAGEAGTVVGVDLQRIDPIEGVETVRGDMTEDATREKVRALVGEADVVISDMAPNMTGEYSLDHARSVHLARMAFETALDLLAPNGDLVAKVFEGPDTDDLRADIDREFEYVRTIHPDASRDSSSELFMVAKGRLTAPVREGDTLEVEIDNLGDEGDGVAKVDGYTLFVSGAEPGDAPEVRVTDVKPRFGFAETLE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ITD5</accession>
    <name>IF6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Translation initiation factor 6</fullName>
            <shortName evidence="1">aIF-6</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">eif6</name>
        <name type="ordered locus">NP_1016A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to the 50S ribosomal subunit and prevents its association with the 30S ribosomal subunit to form the 70S initiation complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322234.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ITD5"/>
    <dbReference type="SMR" id="Q3ITD5"/>
    <dbReference type="STRING" id="348780.NP_1016A"/>
    <dbReference type="EnsemblBacteria" id="CAI48599">
        <property type="protein sequence ID" value="CAI48599"/>
        <property type="gene ID" value="NP_1016A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703272"/>
    <dbReference type="KEGG" id="nph:NP_1016A"/>
    <dbReference type="eggNOG" id="arCOG04176">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071894_1_0_2"/>
    <dbReference type="OrthoDB" id="33582at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.75.10.10">
        <property type="entry name" value="L-arginine/glycine Amidinotransferase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00032">
        <property type="entry name" value="eIF_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002769">
        <property type="entry name" value="eIF6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00323">
        <property type="entry name" value="eIF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003128">
        <property type="entry name" value="PRK04046.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10784">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01912">
        <property type="entry name" value="eIF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006413">
        <property type="entry name" value="IF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00654">
        <property type="entry name" value="eIF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55909">
        <property type="entry name" value="Pentein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000259432" description="Translation initiation factor 6">
        <location>
            <begin position="1"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00032"/>
        </source>
    </evidence>
    <sequence length="221" mass="23134" checksum="BCDAF57AE45A7550" modified="2005-11-08" version="1">MLRAALSGSSYVGVFATATDEYLLVRPDIEESLQSEFETELDVTAVPTTIAGSGTVGSLAVGNENGLLVTERVTDRERDRLETETGLPVVELPGNINAAGNVVLANNEGAFVHPDLSRDALSAIEEALSVPVERGRIADVQTVGTAAVVTDDGVLSHPKTTDEKLDFLEELFGVYADIGTINYGGPLVGSGLVANNTGYIAGEETTGPELGRIEDALGFIQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-30" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IUD7</accession>
    <name>RS10_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein uS10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">30S ribosomal protein S10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps10</name>
        <name type="ordered locus">NP_0306A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the binding of tRNA to the ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321882.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IUD7"/>
    <dbReference type="SMR" id="Q3IUD7"/>
    <dbReference type="STRING" id="348780.NP_0306A"/>
    <dbReference type="EnsemblBacteria" id="CAI48244">
        <property type="protein sequence ID" value="CAI48244"/>
        <property type="gene ID" value="NP_0306A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703165"/>
    <dbReference type="KEGG" id="nph:NP_0306A"/>
    <dbReference type="eggNOG" id="arCOG01758">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122625_0_1_2"/>
    <dbReference type="OrthoDB" id="371736at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.600:FF:000004">
        <property type="entry name" value="30S ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.600">
        <property type="entry name" value="Ribosomal protein S10 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00508">
        <property type="entry name" value="Ribosomal_uS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001848">
        <property type="entry name" value="Ribosomal_uS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018268">
        <property type="entry name" value="Ribosomal_uS10_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027486">
        <property type="entry name" value="Ribosomal_uS10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036838">
        <property type="entry name" value="Ribosomal_uS10_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005729">
        <property type="entry name" value="Ribosomal_uS10_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01046">
        <property type="entry name" value="uS10_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11700">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S10 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00338">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00971">
        <property type="entry name" value="RIBOSOMALS10"/>
    </dbReference>
    <dbReference type="SMART" id="SM01403">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54999">
        <property type="entry name" value="Ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00361">
        <property type="entry name" value="RIBOSOMAL_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000237125" description="Small ribosomal subunit protein uS10">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="34"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="102" mass="11469" checksum="D382CFC5281BFBA3" modified="2005-11-08" version="1">MQQARVRLAGTAPEDLDDICDDVREIADKTGVTLSGPVPLPTKTLEIPARKSPDGEGTATWEHWEMRVHKRLIDIDADERALRQLMRIQVPNDVSIEIVLED</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IUP8</accession>
    <name>HIS5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Imidazole glycerol phosphate synthase subunit HisH</fullName>
            <ecNumber evidence="1">4.3.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">IGP synthase glutaminase subunit</fullName>
            <ecNumber evidence="1">3.5.1.2</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">IGP synthase subunit HisH</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">ImGP synthase subunit HisH</fullName>
            <shortName evidence="1">IGPS subunit HisH</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisH</name>
        <name type="ordered locus">NP_0082A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the synthesis of IGP and AICAR. The resulting ammonia molecule is channeled to the active site of HisF.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine = D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="4.3.2.10"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of HisH and HisF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="4.3.2.10" evidence="1"/>
    <dbReference type="EC" id="3.5.1.2" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321771.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IUP8"/>
    <dbReference type="SMR" id="Q3IUP8"/>
    <dbReference type="STRING" id="348780.NP_0082A"/>
    <dbReference type="EnsemblBacteria" id="CAI48132">
        <property type="protein sequence ID" value="CAI48132"/>
        <property type="gene ID" value="NP_0082A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702821"/>
    <dbReference type="KEGG" id="nph:NP_0082A"/>
    <dbReference type="eggNOG" id="arCOG00089">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071837_2_1_2"/>
    <dbReference type="OrthoDB" id="33401at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000107">
        <property type="term" value="F:imidazoleglycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01748">
        <property type="entry name" value="GATase1_IGP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00278">
        <property type="entry name" value="HisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010139">
        <property type="entry name" value="Imidazole-glycPsynth_HisH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01855">
        <property type="entry name" value="IMP_synth_hisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42701">
        <property type="entry name" value="IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE SUBUNIT HISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42701:SF1">
        <property type="entry name" value="IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE SUBUNIT HISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000495">
        <property type="entry name" value="Amidotransf_hisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01211">
        <property type="entry name" value="GATase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000231776" description="Imidazole glycerol phosphate synthase subunit HisH">
        <location>
            <begin position="1"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-1" evidence="1">
        <location>
            <begin position="9"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="193"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00278"/>
        </source>
    </evidence>
    <sequence length="215" mass="22978" checksum="C9C8B2043E03E813" modified="2005-11-08" version="1">MDTTDRTAEVVLVDYGLGNLRSVTRGLERAGAEVTLSADPDDFSAADGIVLPGVGAFSEGMENAGPFREALVAAAADGQPLFGICLGMQMLLTSSEEAETVGQGDVRGLDLVPGRNVRFDEGQKVPHMGWNELNVARDHPIVEGIDGEYAYFVHSYYAAPDDDAAVVATTDYSVEFPAIVANEAGNVFGTQFHPEKSGETGLQILQNFVDYCLER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETA9</accession>
    <name>A0A1U7ETA9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0068A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETA9"/>
    <dbReference type="STRING" id="348780.NP_0068A"/>
    <dbReference type="EnsemblBacteria" id="CAI48125">
        <property type="protein sequence ID" value="CAI48125"/>
        <property type="gene ID" value="NP_0068A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_0068A"/>
    <dbReference type="eggNOG" id="arCOG07982">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1493019_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046783">
        <property type="entry name" value="HTH_63"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20575">
        <property type="entry name" value="HTH_63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="180" mass="19802" checksum="5FD551AC428550D1" modified="2017-05-10" version="1">MQYISVNSVSLPYRWVAENLLFVYTSRSSMLRGLDFRGTGDKAEQLIIKVADREPVSGEITLYLDEAADGDAERRQQQLRDGFRQLRAAGVLEDISVRTWQESDVASCYEEFVDAVGKEELTPHFRSLAGGNAIDVPCAAVAIRRDGDLAGLYPRSDGDDEQTPVDCLQALCAGDAVDPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETE4</accession>
    <name>A0A1U7ETE4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0148A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48165.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321804.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETE4"/>
    <dbReference type="STRING" id="348780.NP_0148A"/>
    <dbReference type="EnsemblBacteria" id="CAI48165">
        <property type="protein sequence ID" value="CAI48165"/>
        <property type="gene ID" value="NP_0148A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700772"/>
    <dbReference type="KEGG" id="nph:NP_0148A"/>
    <dbReference type="eggNOG" id="arCOG04679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108336_0_0_2"/>
    <dbReference type="OrthoDB" id="307144at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055685">
        <property type="entry name" value="DUF7261"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23922">
        <property type="entry name" value="DUF7261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48165.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="174" mass="17708" checksum="02FC4C284F64F425" modified="2017-05-10" version="1">MGLARLNRGQLVLVAAAVIAIGLVPILFAYLQLGFHPDVDRTPAVAGDDAVAYLDRSVHDAAAATAGDYAAHESDDLADAVRDSIDDDIEALARSRLEEGVGYEVTYNATAAAGWEGAACETGPGARFGDCAADGGVVVQPRAGDAVLVAVAFDVRAIGPESTAELTVVIDVGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETG6</accession>
    <name>A0A1U7ETG6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">PgpA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0208A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321834.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETG6"/>
    <dbReference type="STRING" id="348780.NP_0208A"/>
    <dbReference type="EnsemblBacteria" id="CAI48195">
        <property type="protein sequence ID" value="CAI48195"/>
        <property type="gene ID" value="NP_0208A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700788"/>
    <dbReference type="KEGG" id="nph:NP_0208A"/>
    <dbReference type="eggNOG" id="arCOG06211">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139545_0_0_2"/>
    <dbReference type="OrthoDB" id="213736at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008962">
        <property type="term" value="F:phosphatidylglycerophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058290">
        <property type="entry name" value="DUF7984"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036681">
        <property type="entry name" value="PgpA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25945">
        <property type="entry name" value="DUF7984"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101307">
        <property type="entry name" value="YutG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7984" evidence="2">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="162" mass="17213" checksum="8464269B06D7B7A4" modified="2017-05-10" version="1">MDVTPSSVSAEHERLRENERVPEHINDVRVRLGDAFGVEVTPVSPAQYRREVDAVFADGDRAVNVAALCGLLRSLDCHGDYPGFVVDEFLGRKLAATIAGGEPFALLAEATFHVADIHTHGGADEAAGADDLEAALAAGFQTRLPGWEWTEGESPFGVTGNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETK2</accession>
    <name>A0A1U7ETK2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HAD superfamily hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0292A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48237.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321876.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETK2"/>
    <dbReference type="EnsemblBacteria" id="CAI48237">
        <property type="protein sequence ID" value="CAI48237"/>
        <property type="gene ID" value="NP_0292A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700812"/>
    <dbReference type="KEGG" id="nph:NP_0292A"/>
    <dbReference type="eggNOG" id="arCOG10136">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1431592_0_0_2"/>
    <dbReference type="OrthoDB" id="337956at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008253">
        <property type="term" value="F:5'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009264">
        <property type="term" value="P:deoxyribonucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010708">
        <property type="entry name" value="5'(3')-deoxyribonucleotidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06941">
        <property type="entry name" value="NT5C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="15"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR610708-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48237.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="188" mass="20773" checksum="39C76ED90D46C7A2" modified="2017-05-10" version="1">MTDRSLPTGSTLLVDLDGVVARQLPRLCTHLREAYDHNVAPDDIDEWSYDVPEADGHVGDIIQGLMRERPEWYFGGMDPQPGVADALSSLQSHYRVEIATHRIPETHDISKAWLDDHGIPYDDFHERVPRNKGAVPGDALIDDYHGNVANALAAGKAGLLMRQPYSDPGACDGAHVVTSWDEVTRLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETL7</accession>
    <name>A0A1U7ETL7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Signal peptide peptidase SppA</fullName>
            <ecNumber evidence="7">3.4.21.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">sppA1</name>
        <name evidence="7" type="ordered locus">NP_0348A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S49 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48265.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETL7"/>
    <dbReference type="STRING" id="348780.NP_0348A"/>
    <dbReference type="EnsemblBacteria" id="CAI48265">
        <property type="protein sequence ID" value="CAI48265"/>
        <property type="gene ID" value="NP_0348A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_0348A"/>
    <dbReference type="eggNOG" id="arCOG01311">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046540_0_2_2"/>
    <dbReference type="OrthoDB" id="31107at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07023">
        <property type="entry name" value="S49_Sppa_N_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004635">
        <property type="entry name" value="Pept_S49_SppA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002142">
        <property type="entry name" value="Peptidase_S49"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047272">
        <property type="entry name" value="S49_SppA_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00706">
        <property type="entry name" value="SppA_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33209:SF1">
        <property type="entry name" value="PEPTIDASE S49 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33209">
        <property type="entry name" value="PROTEASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01343">
        <property type="entry name" value="Peptidase_S49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S49" evidence="6">
        <location>
            <begin position="141"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48265.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="332" mass="34978" checksum="32D031C05407E34D" modified="2017-05-10" version="1">MSRIATASRVVLGVFVAAVVAVAGWVIFIEVPTTMADLVGVVFVIVAATVGLRIGGNIATSLAPGYNVAEVAVEGPITRDGGGGMPLSPPGSPGADDIVEQIEDADADDNVEALLLRLNTPGGAVVPSDDIRLAAEAFDGPTVAYTTDACASGGYWIASGCDELWARRGSVVGSIGVRGSRMTAAELLDRAGVEYEQLTAGEYKEAGVPFDDLGDDERQYLQGIVDDYYDQFVETVAEGREMEPSAVRETEAKVFLGEEAFERGLVDDLGTKDEVCERLEEVLGTEVETTELEPQQGLAARLQGGAERVAYAFGAGLAGRFVDAEGEFRFRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETM3</accession>
    <name>A0A1U7ETM3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0350A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321904.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETM3"/>
    <dbReference type="STRING" id="348780.NP_0350A"/>
    <dbReference type="EnsemblBacteria" id="CAI48266">
        <property type="protein sequence ID" value="CAI48266"/>
        <property type="gene ID" value="NP_0350A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700826"/>
    <dbReference type="KEGG" id="nph:NP_0350A"/>
    <dbReference type="eggNOG" id="arCOG15189">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_209473_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="60" mass="5655" checksum="E71A80CA86CD31A1" modified="2017-05-10" version="1">MATDTQKLGAGLAALVVGSVLILSTSFVNTPVPGAAAGVAAMLMAVGSLLVGLSEKGAGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETN2</accession>
    <name>A0A1U7ETN2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable carbon-nitrogen hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0374A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321916.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETN2"/>
    <dbReference type="STRING" id="348780.NP_0374A"/>
    <dbReference type="EnsemblBacteria" id="CAI48278">
        <property type="protein sequence ID" value="CAI48278"/>
        <property type="gene ID" value="NP_0374A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702498"/>
    <dbReference type="KEGG" id="nph:NP_0374A"/>
    <dbReference type="eggNOG" id="arCOG00062">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030130_3_1_2"/>
    <dbReference type="OrthoDB" id="41015at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001110">
        <property type="entry name" value="UPF0012_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088:SF27">
        <property type="entry name" value="DEAMINATED GLUTATHIONE AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088">
        <property type="entry name" value="NITRILASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01227">
        <property type="entry name" value="UPF0012"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="272" mass="29598" checksum="51BB66FDCB581E46" modified="2017-05-10" version="1">MRLALAQLEITPRDVEENVTRAVDAIADAAAAGADCVVLPEIFDVGYFAFDSYERYAQPLDGATHQRLREAAVEFDVAVLAGTVVEDLAASADAAQPVPAEEGLANTAVLFDSDGERQLVYRKHHLFGYESAEAELLVPGERLPTANICGHTVAVTTCYDLRFPELYRQLAEKDVTMVLVPSAWPYPRIEHWKLLPRARAVENLLYVGAVNGSATFDDASLVGRSTIYDPWGTPVAAADDGPTLVVADCPPDRVADVRSEFPALDDRRDGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETS5</accession>
    <name>A0A1U7ETS5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0450A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321952.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETS5"/>
    <dbReference type="STRING" id="348780.NP_0450A"/>
    <dbReference type="EnsemblBacteria" id="CAI48316">
        <property type="protein sequence ID" value="CAI48316"/>
        <property type="gene ID" value="NP_0450A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700859"/>
    <dbReference type="KEGG" id="nph:NP_0450A"/>
    <dbReference type="HOGENOM" id="CLU_2821025_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055957">
        <property type="entry name" value="DUF7535"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24379">
        <property type="entry name" value="DUF7535"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="66" mass="7366" checksum="E6189D7C6FAFAEA9" modified="2017-05-10" version="1">MADETQQNEGVLGALRRPASNGELRNPKIAAAAVLLLVLLPLIPFFVVVWRISKTLRGVRKRVSWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETV3</accession>
    <name>A0A1U7ETV3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0526A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321990.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETV3"/>
    <dbReference type="STRING" id="348780.NP_0526A"/>
    <dbReference type="EnsemblBacteria" id="CAI48354">
        <property type="protein sequence ID" value="CAI48354"/>
        <property type="gene ID" value="NP_0526A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700886"/>
    <dbReference type="KEGG" id="nph:NP_0526A"/>
    <dbReference type="eggNOG" id="arCOG04509">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_206261_0_0_2"/>
    <dbReference type="OrthoDB" id="214577at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055757">
        <property type="entry name" value="DUF7333"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24020">
        <property type="entry name" value="DUF7333"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="62" mass="6348" checksum="087F5B2D7314438C" modified="2017-05-10" version="1">MQFNLPVALGALLVIVAIGAGGLVASGMMILETTLMMVVPSMVVFGLIAFGLGVKHGEYRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETX6</accession>
    <name>A0A1U7ETX6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0582A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322018.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETX6"/>
    <dbReference type="STRING" id="348780.NP_0582A"/>
    <dbReference type="EnsemblBacteria" id="CAI48382">
        <property type="protein sequence ID" value="CAI48382"/>
        <property type="gene ID" value="NP_0582A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700896"/>
    <dbReference type="KEGG" id="nph:NP_0582A"/>
    <dbReference type="eggNOG" id="arCOG03681">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1727293_0_0_2"/>
    <dbReference type="OrthoDB" id="232490at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="151" mass="16559" checksum="473FE3BC871689E8" modified="2017-05-10" version="1">METPTVDDWGEAVTLARSLGHDGRVRVPKAAEPTLPESVSLRHSNFPWSIHRGAVAVYRESERGDHLQIREYPACWEVSVDSYNPHYRPVSHAAVDIPAQMLLAMGALTPLDGYRWVFGDRLPPVFAPVSYSLSALGSASRLGKKLLPGVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETZ1</accession>
    <name>A0A1U7ETZ1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0632A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETZ1"/>
    <dbReference type="EnsemblBacteria" id="CAI48407">
        <property type="protein sequence ID" value="CAI48407"/>
        <property type="gene ID" value="NP_0632A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_0632A"/>
    <dbReference type="eggNOG" id="arCOG06331">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2629834_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055978">
        <property type="entry name" value="DUF7556"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24433">
        <property type="entry name" value="DUF7556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="77" mass="8395" checksum="05CFD8370E46B56F" modified="2017-05-10" version="1">MEDRGVDNGFVTLLTLLPVGSGMRDIHDVAAVGRTESIQESYDYCEGESAFIIADTATDDAWVAISRGDEAAVEEWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU67</accession>
    <name>A0A1U7EU67_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PHP domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0818A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322136.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU67"/>
    <dbReference type="STRING" id="348780.NP_0818A"/>
    <dbReference type="EnsemblBacteria" id="CAI48500">
        <property type="protein sequence ID" value="CAI48500"/>
        <property type="gene ID" value="NP_0818A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700970"/>
    <dbReference type="KEGG" id="nph:NP_0818A"/>
    <dbReference type="eggNOG" id="arCOG00306">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1036680_0_0_2"/>
    <dbReference type="OrthoDB" id="190669at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13263">
        <property type="entry name" value="PHP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="PHP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="251" mass="28564" checksum="357F25EFF51D2A04" modified="2017-05-10" version="1">MDRFRLDAHVKVLDETVAERAKARGLDGLVYAPHFTRLPDIRERAAEFTDDDLLVVPGREVFTGDWRNRKHVLAVGLEAPVPDFITLEAAMAEFDRQDAAVLAPHPEFATVSLEAHDIRAHRDRIHAVEVYNPKHFATHNERARELAAEFELPAFGSSYAHLPGTVGEVWTGFDRRFETADELVAALREGVPRGVYHRDGPRHRLRCMAEFGHLFYENSWGKIDRLFLSGTEPTHPDHIAYDGRFDDVSVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU82</accession>
    <name>A0A1U7EU82_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Mrp-type sodium/proton antiporter system subunit D2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">mrpD2</name>
        <name evidence="9" type="synonym">tp49h</name>
        <name evidence="9" type="ordered locus">NP_0828A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322141.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU82"/>
    <dbReference type="STRING" id="348780.NP_0828A"/>
    <dbReference type="EnsemblBacteria" id="CAI48505">
        <property type="protein sequence ID" value="CAI48505"/>
        <property type="gene ID" value="NP_0828A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703334"/>
    <dbReference type="KEGG" id="nph:NP_0828A"/>
    <dbReference type="eggNOG" id="arCOG01537">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030481_0_0_2"/>
    <dbReference type="OrthoDB" id="198789at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052175">
        <property type="entry name" value="ComplexI-like_HydComp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_TM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009310">
        <property type="entry name" value="PRK12668.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42682">
        <property type="entry name" value="HYDROGENASE-4 COMPONENT F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42682:SF4">
        <property type="entry name" value="NADH-UBIQUINONE_PLASTOQUINONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01434">
        <property type="entry name" value="NADHDHGNASE5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="126"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="253"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="286"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="316"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="340"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="399"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="436"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="476"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="584"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:quinone oxidoreductase/Mrp antiporter transmembrane" evidence="8">
        <location>
            <begin position="116"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI48505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="607" mass="63248" checksum="5666004470996C84" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU93</accession>
    <name>A0A1U7EU93_NATPD</name>
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        <submittedName>
            <fullName evidence="2">DUF82 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0852A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A1U7EU93"/>
    <dbReference type="STRING" id="348780.NP_0852A"/>
    <dbReference type="EnsemblBacteria" id="CAI48517">
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        <property type="gene ID" value="NP_0852A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700976"/>
    <dbReference type="KEGG" id="nph:NP_0852A"/>
    <dbReference type="eggNOG" id="arCOG04290">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112469_0_0_2"/>
    <dbReference type="OrthoDB" id="1266at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002782">
        <property type="entry name" value="Mut7-C_RNAse_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39081">
        <property type="entry name" value="MUT7-C DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39081:SF1">
        <property type="entry name" value="MUT7-C RNASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01927">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mut7-C RNAse" evidence="1">
        <location>
            <begin position="13"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="159" mass="17839" checksum="B9E30EEFA2EF04D8" modified="2017-05-10" version="1">MSDTRRCRPASDRLLLDVMLGKLAVYLRCCGYDTVYAGDRDIEADDRLRELAATEGRVLITRDTELAARVDRGLLLGTRDVEAQLSELHEMGVELTVDERPTRCGRCNGHLEPVGADEPTPEYAPDSAGTDCWRCHACEQVFWKGSHWERMQETIANVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUD2</accession>
    <name>A0A1U7EUD2_NATPD</name>
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        <submittedName>
            <fullName evidence="2">AlkP-core domain protein</fullName>
        </submittedName>
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        <name evidence="2" type="ordered locus">NP_0946A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
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            <taxon>Methanobacteriati</taxon>
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            <taxon>Stenosarchaea group</taxon>
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            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
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            </authorList>
            <dbReference type="PubMed" id="16169924"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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    <dbReference type="EnsemblBacteria" id="CAI48564">
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    </dbReference>
    <dbReference type="GeneID" id="3701006"/>
    <dbReference type="KEGG" id="nph:NP_0946A"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="GAG_Ulvan_Degrading_Sulfatases"/>
    </dbReference>
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        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43751">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43751:SF3">
        <property type="entry name" value="SULFATASE N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sulfatase N-terminal" evidence="1">
        <location>
            <begin position="5"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="495" mass="54555" checksum="5FAFD480089FCA8D" modified="2017-05-10" version="1">MAADPNVLVVVLDTVRKDHLTPYGYDRPTTPALASFADEARVYEEAVSQAPWTLPAHASLFTGQYPSVHGATQESPYLSAETATLAEAVSASGYTTTCFSSNAWITPYTRLTAGFDEQDSFFEALPGSVLATAAADLWSWVLDSRLRPLADRLVAAGNAVHERLAARGRAASRTPAIVDRTQQFIEGADGPWFAFLNLMDAHLPYYPPEQYRQQFAPDVDPGAVCQNSKAYNAGAREVDEAEFEAIRRLYDAELRHLDAELGRLFEFLRTSGRWDDTAVVVCADHGELHGEFGRYGHEFAVYEPLVNVPLLVDAPGIEPGRDRQQVELLDLYDTVLDIAGAAEAASSVGARPFEPERSLCRPGRDIEDGDYGFIEYDRPVVELEQLERKAAAAGIELDEDGRFYSRMRAARRPDGKYIRNERLSDEGYRLDSDSGESTEAAADDPIIEATEAALCRFEDRVAVGWDGHTQGDGGDPLGRMDETAKSRLRDLGYLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUD8</accession>
    <name>A0A1U7EUD8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">AI-2E family transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">NP_0962A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUD8"/>
    <dbReference type="STRING" id="348780.NP_0962A"/>
    <dbReference type="EnsemblBacteria" id="CAI48572">
        <property type="protein sequence ID" value="CAI48572"/>
        <property type="gene ID" value="NP_0962A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701012"/>
    <dbReference type="KEGG" id="nph:NP_0962A"/>
    <dbReference type="eggNOG" id="arCOG02642">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052137_0_0_2"/>
    <dbReference type="OrthoDB" id="137390at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002549">
        <property type="entry name" value="AI-2E-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 245"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01594">
        <property type="entry name" value="AI-2E_transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="128"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="188"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="218"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="255"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="294"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="331" mass="35022" checksum="46BCB195426A52D5" modified="2017-05-10" version="1">MSSLRHRRYVLGGLLAAIGVLAAAVLWNVVGTVFFAVTVAYVLYPARQRLVNRGVPRRVASGLLTAAAFLTVVVLLAPIAWTIFRQRGAILEFLNNLPDELPVEAFGFVVTIDVADLVDTTADTLQSIAISLAADAIFLALQLLMFALVLYGLLLRPNAVGRAAFRVTPSPYHDILVALHRRIAGTLYALYVIQAATAVITFPLALIVFYLLGYPDVFVLAVLAALLQFIPIVGPGMLAAGLAGHDVLVGMPHRAVGVIILGPLFVGLLPDLLVRPRLAASKAKLPASLYFVGFVGGVLTVGVIGIIAGPLVVALLVEAVNLLSERNQSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUE2</accession>
    <name>A0A1U7EUE2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0970A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322211.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUE2"/>
    <dbReference type="STRING" id="348780.NP_0970A"/>
    <dbReference type="EnsemblBacteria" id="CAI48576">
        <property type="protein sequence ID" value="CAI48576"/>
        <property type="gene ID" value="NP_0970A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701015"/>
    <dbReference type="KEGG" id="nph:NP_0970A"/>
    <dbReference type="eggNOG" id="arCOG06339">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1021634_0_0_2"/>
    <dbReference type="OrthoDB" id="242870at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="258"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="272" mass="27114" checksum="1DDA020052709C36" modified="2017-05-10" version="1">MTTAQTGLVVALTAVLLATVAGAGVVAADDDADALAVDVEQPDDVVLTVTDDGDAVENATVTVSIAEDNETDTSNVTYDETGTHTTDANGTVSLSPPESTVDVTVAATDDDRTTTTEATLTAAADDEAFGQQVSAFVHQLLHGDETNATVGQQVSDFVTDHNPGNASTGGPWGPPAHAGPDSERPGHAGPGGDAERPGADAPRGPGSTDRHEQAGGPATDAPDQNSTDDRRGPADGDRSGQATDGDDDEETTTPERGSGSGSGGNSGSGPGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUF1</accession>
    <name>A0A1U7EUF1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DUF424 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0996A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322224.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUF1"/>
    <dbReference type="STRING" id="348780.NP_0996A"/>
    <dbReference type="EnsemblBacteria" id="CAI48589">
        <property type="protein sequence ID" value="CAI48589"/>
        <property type="gene ID" value="NP_0996A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701025"/>
    <dbReference type="KEGG" id="nph:NP_0996A"/>
    <dbReference type="eggNOG" id="arCOG04051">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_174522_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1860.10">
        <property type="entry name" value="uncharacterized conserved protein from methanopyrus kandleri domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007355">
        <property type="entry name" value="DUF424"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04242">
        <property type="entry name" value="DUF424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="98" mass="10450" checksum="DA9329BE5F32EF8D" modified="2017-05-10" version="1">MTLLLCERSTPEGLLVSVCDADVLGETFDNGDVSLTVEPEFYDGETASHEEVTASLADAAVANLVGHEAVGLAVEHGFVAEANVLEFEETVHAQYLRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUF7</accession>
    <name>A0A1U7EUF7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">NikR family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_1034A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322243.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUF7"/>
    <dbReference type="STRING" id="348780.NP_1034A"/>
    <dbReference type="EnsemblBacteria" id="CAI48608">
        <property type="protein sequence ID" value="CAI48608"/>
        <property type="gene ID" value="NP_1034A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701032"/>
    <dbReference type="KEGG" id="nph:NP_1034A"/>
    <dbReference type="eggNOG" id="arCOG01008">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113319_0_0_2"/>
    <dbReference type="OrthoDB" id="9459at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22231">
        <property type="entry name" value="RHH_NikR_HicB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1150">
        <property type="entry name" value="ACT-like. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="entry name" value="Met repressor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027271">
        <property type="entry name" value="Acetolactate_synth/TF_NikR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050192">
        <property type="entry name" value="CopG/NikR_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014864">
        <property type="entry name" value="TF_NikR_Ni-bd_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34719">
        <property type="entry name" value="NICKEL-RESPONSIVE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34719:SF3">
        <property type="entry name" value="NICKEL-RESPONSIVE REGULATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08753">
        <property type="entry name" value="NikR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="Ribbon-helix-helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor NikR nickel binding C-terminal" evidence="4">
        <location>
            <begin position="54"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI48608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="137" mass="15040" checksum="38BC4C04C57C440B" modified="2017-05-10" version="1">MPVVSVSMPASLLDRLDEFIDEHDYSGRSEAVREGTRKVLTEFDDQSVPTSPAVCIVAVMFDHDTDAESSLSTLRHEHDELVTSNVHSHAGEACLELFVAEGEVDAIGTFVSRVRTVDGVGSVEYTFVDDNDEIIPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUJ7</accession>
    <name>A0A1U7EUJ7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ATP-dependent cobaltochelatase subunit ChlID</fullName>
            <ecNumber evidence="6">6.6.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">chlID</name>
        <name evidence="6" type="ordered locus">NP_1094A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Mg-chelatase subunits D/I family.</text>
    </comment>
    <dbReference type="EC" id="6.6.1.2" evidence="6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322274.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUJ7"/>
    <dbReference type="STRING" id="348780.NP_1094A"/>
    <dbReference type="EnsemblBacteria" id="CAI48638">
        <property type="protein sequence ID" value="CAI48638"/>
        <property type="gene ID" value="NP_1094A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702605"/>
    <dbReference type="KEGG" id="nph:NP_1094A"/>
    <dbReference type="eggNOG" id="arCOG00438">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016684_6_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051116">
        <property type="term" value="F:cobaltochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.80">
        <property type="entry name" value="Magnesium chelatase subunit I, C-Terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041628">
        <property type="entry name" value="ChlI/MoxR_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052989">
        <property type="entry name" value="Mg-chelatase_DI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000523">
        <property type="entry name" value="Mg_chelatse_chII-like_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
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        <property type="entry name" value="CHELATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MG-PROTOPORPHYRIN IX CHELATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA_lid_2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Mg_chelatase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="VWA_2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="vWA-like"/>
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        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="5">
        <location>
            <begin position="536"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="316"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="334"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="365"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="482"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI48638.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="708" mass="73095" checksum="07C0209817B192A0" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUR4</accession>
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        <recommendedName>
            <fullName evidence="12">DNA topoisomerase 1</fullName>
            <ecNumber evidence="12">5.6.2.1</ecNumber>
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        <alternativeName>
            <fullName evidence="12">DNA topoisomerase I</fullName>
        </alternativeName>
    </protein>
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        <name evidence="12 16" type="primary">topA</name>
        <name evidence="16" type="ordered locus">NP_1222A</name>
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        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="12">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the type IA topoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="12"/>
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        <property type="protein sequence ID" value="CAI48702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
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        <property type="protein sequence ID" value="CAI48702"/>
        <property type="gene ID" value="NP_1222A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703277"/>
    <dbReference type="KEGG" id="nph:NP_1222A"/>
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        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="eggNOG" id="arCOG06233">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002929_1_4_2"/>
    <dbReference type="OrthoDB" id="30963at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
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        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00186">
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    <dbReference type="CDD" id="cd03362">
        <property type="entry name" value="TOPRIM_TopoIA_TopoIII"/>
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        <property type="entry name" value="DNA topoisomerase"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bacterial Topoisomerase I, domain 1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.290.10">
        <property type="entry name" value="Topoisomerase I, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Topoisom_1_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Topo_IA"/>
    </dbReference>
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        <property type="entry name" value="Topo_IA_2"/>
    </dbReference>
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        <property type="entry name" value="Topo_IA_AS"/>
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    <dbReference type="InterPro" id="IPR013497">
        <property type="entry name" value="Topo_IA_cen"/>
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        <property type="entry name" value="Topo_IA_cen_sub1"/>
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        <property type="entry name" value="Topo_IA_cen_sub2"/>
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    <dbReference type="InterPro" id="IPR013826">
        <property type="entry name" value="Topo_IA_cen_sub3"/>
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    <dbReference type="InterPro" id="IPR023405">
        <property type="entry name" value="Topo_IA_core_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003602">
        <property type="entry name" value="Topo_IA_DNA-bd_dom"/>
    </dbReference>
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        <property type="entry name" value="Topo_IA_Znf"/>
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        <property type="entry name" value="Topoisom_1_IA"/>
    </dbReference>
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        <property type="entry name" value="TOPRIM_dom"/>
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        <property type="entry name" value="TOPRIM_TopoIII"/>
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        <property type="entry name" value="PRK07220.1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA TOPOISOMERASE 1"/>
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    </dbReference>
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        <property type="entry name" value="PROKARYOTIC DNA TOPOISOMERASE"/>
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    </dbReference>
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        <property type="entry name" value="HHH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Topoisom_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01396">
        <property type="entry name" value="Zn_ribbon_Top1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00417">
        <property type="entry name" value="PRTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00437">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00436">
        <property type="entry name" value="TOP1Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56712">
        <property type="entry name" value="Prokaryotic type I DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00396">
        <property type="entry name" value="TOPO_IA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52039">
        <property type="entry name" value="TOPO_IA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11 12 16" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 12" id="KW-0799">Topoisomerase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Toprim" evidence="14">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Topo IA-type catalytic" evidence="15">
        <location>
            <begin position="150"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with DNA" evidence="12">
        <location>
            <begin position="188"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="358"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="464"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="12">
        <location>
            <position position="316"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="12">
        <location>
            <position position="48"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="12">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="12">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="12">
        <location>
            <position position="318"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="12">
        <location>
            <position position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS52039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAI48702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="832" mass="92971" checksum="71888579FE4D4BC8" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUW7</accession>
    <name>A0A1U7EUW7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">PaaI family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_1402A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322427.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUW7"/>
    <dbReference type="STRING" id="348780.NP_1402A"/>
    <dbReference type="EnsemblBacteria" id="CAI48792">
        <property type="protein sequence ID" value="CAI48792"/>
        <property type="gene ID" value="NP_1402A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701138"/>
    <dbReference type="KEGG" id="nph:NP_1402A"/>
    <dbReference type="eggNOG" id="arCOG00777">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089876_7_1_2"/>
    <dbReference type="OrthoDB" id="202256at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016289">
        <property type="term" value="F:acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03443">
        <property type="entry name" value="PaaI_thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052723">
        <property type="entry name" value="Acyl-CoA_thioesterase_PaaI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003736">
        <property type="entry name" value="PAAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00369">
        <property type="entry name" value="unchar_dom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42856">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE PAAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42856:SF1">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE PAAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioesterase" evidence="3">
        <location>
            <begin position="46"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="121"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="147" mass="15143" checksum="ED0DD4FE2A2F5318" modified="2017-05-10" version="1">MDVTDMFSEMPFNDHLGIEVTEAADGRAVGHLELGTEHSSNPNRLVAHGGVAYALADTVGGAAVISANFTVTPTIDMRIDYLSPATGGRLDAEAEVVRNGDSVAAVDIEVTDESGTTVATARGTYKTGGGNGGSAWHSGDDEYTNGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUW8</accession>
    <name>A0A1U7EUW8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Small CPxCG-related zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1378A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUW8"/>
    <dbReference type="STRING" id="348780.NP_1378A"/>
    <dbReference type="EnsemblBacteria" id="CAI48780">
        <property type="protein sequence ID" value="CAI48780"/>
        <property type="gene ID" value="NP_1378A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_1378A"/>
    <dbReference type="HOGENOM" id="CLU_2327339_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="98" mass="9670" checksum="A6CEA41988655D0B" modified="2017-05-10" version="1">MTANAFAAAVPVLGVPQCSNCGADVAADAEYCQGCGAAFGDATGRDWNVKRGVGVGVLVAVFFAFLVPLSGLLLEAAILLAIPAGVLAGVLTAVYSSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV04</accession>
    <name>A0A1U7EV04_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.117</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">dapD</name>
        <name evidence="3" type="ordered locus">NP_1494A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.117" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322472.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV04"/>
    <dbReference type="STRING" id="348780.NP_1494A"/>
    <dbReference type="EnsemblBacteria" id="CAI48838">
        <property type="protein sequence ID" value="CAI48838"/>
        <property type="gene ID" value="NP_1494A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702658"/>
    <dbReference type="KEGG" id="nph:NP_1494A"/>
    <dbReference type="eggNOG" id="arCOG04556">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050859_0_1_2"/>
    <dbReference type="OrthoDB" id="280332at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008666">
        <property type="term" value="F:2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd03350">
        <property type="entry name" value="LbH_THP_succinylT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.166.10">
        <property type="entry name" value="Tetrahydrodipicolinate-N-succinyltransferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023180">
        <property type="entry name" value="THP_succinylTrfase_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037133">
        <property type="entry name" value="THP_succinylTrfase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050179">
        <property type="entry name" value="Trans_hexapeptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008808">
        <property type="entry name" value="PRK11830.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43300:SF10">
        <property type="entry name" value="2,3,4,5-TETRAHYDROPYRIDINE-2,6-DICARBOXYLATE N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43300">
        <property type="entry name" value="ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14602">
        <property type="entry name" value="Hexapep_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14805">
        <property type="entry name" value="THDPS_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrahydrodipicolinate-N-succinyltransferase chain A" evidence="2">
        <location>
            <begin position="4"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="278" mass="29714" checksum="389F115C3BD9A984" modified="2017-05-10" version="1">MSTLQSDIEDLQHRYENGLTADDAGNEEYALLDEFLAALEAGEVRAAEKRDGEWDANGWVKQGILLNFGLRDIQAHTHGGVDYHDVLPLRETDDLPGRGTRNTPDGTVIRRGAYIGGDAILMSPAFVNIGAYVGDGTLVDSCDTVGSAAQIGENVKLGANTLIGGVLEPVEAAPVVIEDDVSLGAGCRVTSGFVVGEGSVVGENTLLTPRIPVYDLVEEEVMYGELPPERRAFTRFVESSVGDHDLFDGGAYKPAVVATHVEEDTLEATAREDALRDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV13</accession>
    <name>A0A1U7EV13_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1520A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322485.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV13"/>
    <dbReference type="STRING" id="348780.NP_1520A"/>
    <dbReference type="EnsemblBacteria" id="CAI48851">
        <property type="protein sequence ID" value="CAI48851"/>
        <property type="gene ID" value="NP_1520A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701168"/>
    <dbReference type="KEGG" id="nph:NP_1520A"/>
    <dbReference type="eggNOG" id="arCOG11342">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2010153_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058272">
        <property type="entry name" value="DUF7966"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25920">
        <property type="entry name" value="DUF7966"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="123" mass="13211" checksum="9C7AE789C6BBB9A7" modified="2017-05-10" version="1">MSRNDTAERTCRTERQVRTLLSRLAGAAPETVVRRAIAATDDIEAAAAFADGTDLEALAAAVEQVEDKSLKRRGQAALASFRRFRQAAVGNDPTIPPGDTPGENHVHRARGSDLRSGDEPTDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV15</accession>
    <name>A0A1U7EV15_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HTH domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1514A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322482.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV15"/>
    <dbReference type="STRING" id="348780.NP_1514A"/>
    <dbReference type="EnsemblBacteria" id="CAI48848">
        <property type="protein sequence ID" value="CAI48848"/>
        <property type="gene ID" value="NP_1514A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701166"/>
    <dbReference type="KEGG" id="nph:NP_1514A"/>
    <dbReference type="eggNOG" id="arCOG04672">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121279_0_0_2"/>
    <dbReference type="OrthoDB" id="201100at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055770">
        <property type="entry name" value="DUF7346"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24037">
        <property type="entry name" value="DUF7346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="137" mass="14858" checksum="7FA3A3CAEAEAFFB0" modified="2017-05-10" version="1">MTPVEHDGNRYLLRKQSTESSLVYDPETGDERYLPNDELTEVGDSPLSVAAESVPEAARRVLVAVHSDETLGLLVELDERGPLPARELLGRYGLCESDLHGRTAELRAAGLVATADIDGERGYAITDAGRDGLDVLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVC7</accession>
    <name>A0A1U7EVC7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1726A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322587.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVC7"/>
    <dbReference type="STRING" id="348780.NP_1726A"/>
    <dbReference type="EnsemblBacteria" id="CAI48954">
        <property type="protein sequence ID" value="CAI48954"/>
        <property type="gene ID" value="NP_1726A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701239"/>
    <dbReference type="KEGG" id="nph:NP_1726A"/>
    <dbReference type="HOGENOM" id="CLU_1987686_0_0_2"/>
    <dbReference type="OrthoDB" id="380426at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="125" mass="13282" checksum="1C9699B1FBB4A26E" modified="2017-05-10" version="1">MVNGSDGQDVLPDLEAVRPRAYADDDPAAVLRFSNGARLRYRAGETAIFEEWVPPASGSPARSHEVSTPTPHEGTAEPTTDGRPSSRALSDKAIAAVGSYLSFDSRTDAAFTWGEANVDVLFGDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVE6</accession>
    <name>A0A1U7EVE6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC-type transport system permease protein (Probable substrate glutamine/glutamate/polar amino acids)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">abc04p2a</name>
        <name evidence="10" type="ordered locus">NP_1776A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322612.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVE6"/>
    <dbReference type="STRING" id="348780.NP_1776A"/>
    <dbReference type="EnsemblBacteria" id="CAI48979">
        <property type="protein sequence ID" value="CAI48979"/>
        <property type="gene ID" value="NP_1776A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702375"/>
    <dbReference type="KEGG" id="nph:NP_1776A"/>
    <dbReference type="eggNOG" id="arCOG01798">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019602_1_1_2"/>
    <dbReference type="OrthoDB" id="323678at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010065">
        <property type="entry name" value="AA_ABC_transptr_permease_3TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043429">
        <property type="entry name" value="ArtM/GltK/GlnP/TcyL/YhdX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01726">
        <property type="entry name" value="HEQRo_perm_3TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30614:SF0">
        <property type="entry name" value="L-CYSTINE TRANSPORT SYSTEM PERMEASE PROTEIN TCYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30614">
        <property type="entry name" value="MEMBRANE COMPONENT OF AMINO ACID ABC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="32"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="37"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI48979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="262" mass="28405" checksum="2AD05A7088D016D8" modified="2017-05-10" version="1">MEPLALQATDWQFAWVTLQTTDWQFVWENRAILLQGFLVTVLLTLVSVLLGFLAGFPAGAIEVYGRGPLRSAVRTVGVLLRGTPIVVLMVLFFFGLPLPDPGDITFSSITLGNTTIPEFSVGFEAFFAAMLALGLRSAAYQSQVFRGAIQSIDEGQVAAARSVGLSKYEAIRHVVVPQALRRSVPGFQNEFTIVLKDTSIAFAIGLAELLTRGYDLFTQPGQSTAVLEVILAVSLIYFVLTFTTNRALDALSDYYAIPGGDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVJ1</accession>
    <name>A0A1U7EVJ1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable glycosyltransferase, type 1</fullName>
            <ecNumber evidence="2">2.4.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">gth4</name>
        <name evidence="2" type="ordered locus">NP_1922A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.4.-.-" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49052.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322684.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVJ1"/>
    <dbReference type="STRING" id="348780.NP_1922A"/>
    <dbReference type="EnsemblBacteria" id="CAI49052">
        <property type="protein sequence ID" value="CAI49052"/>
        <property type="gene ID" value="NP_1922A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701305"/>
    <dbReference type="KEGG" id="nph:NP_1922A"/>
    <dbReference type="eggNOG" id="arCOG01407">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_2_2_2"/>
    <dbReference type="OrthoDB" id="132546at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03801">
        <property type="entry name" value="GT4_PimA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050194">
        <property type="entry name" value="Glycosyltransferase_grp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45947">
        <property type="entry name" value="SULFOQUINOVOSYL TRANSFERASE SQD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45947:SF3">
        <property type="entry name" value="SULFOQUINOVOSYL TRANSFERASE SQD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="1">
        <location>
            <begin position="195"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49052.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="377" mass="41871" checksum="5F82BB5724D0498F" modified="2017-05-10" version="1">MSEPDDVCVVTHPIGSASESHAETLLDILGAITDVSLVAISVAEESPLREEYDTIEVSRVGTGGPIPIAALRFLANQVRMAMILRRRPEEVVLFFGVTAYLLPIVAAKLAGKTVVLQPRGNVPLTLRLHWERRLPDVLARGLAGVVWSMERLGYHAADAIITYTPSMAEELGLDRFEEKLYPNGARYVDTDEFYPRVPFEERDRVVGFLGRLDEEKNVRTLAAVAKELPEDVTFRFIGDGDLREELEEELAAEIEAGKVEFTGWVDHDEVPRQLSELRLLVLPSEPTEGLPTVILEAMACGTPVLATPVSGVPDVVRQGETSFLMDEVAGEAIARDIEGILDRDDLTELSQYAREHIKAEYSFDAAVRRWRRVLEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVJ2</accession>
    <name>A0A1U7EVJ2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">IS1341-type transposase ISNph23</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">NP_1890A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322668.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVJ2"/>
    <dbReference type="STRING" id="348780.NP_1890A"/>
    <dbReference type="EnsemblBacteria" id="CAI49036">
        <property type="protein sequence ID" value="CAI49036"/>
        <property type="gene ID" value="NP_1890A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701293"/>
    <dbReference type="KEGG" id="nph:NP_1890A"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_3_3_2"/>
    <dbReference type="OrthoDB" id="210698at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF26">
        <property type="entry name" value="TRANSPOSASE, PROBABLY IS605-TNPB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="6">
        <location>
            <begin position="186"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="7">
        <location>
            <begin position="304"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="414" mass="47419" checksum="579EE92759EB3963" modified="2017-05-10" version="1">MEARRALPVKLLVADSDSDALLETIDQYKHCANATSDHCWDDNGYKKTAKYAVKDELYHDLKAEHDLTANLVQQAIFQAVEAVKAGVERLEKNEDTSQPEFTADTARYDKRAATFHDDHVSLSTVDGRIEADYVLPVDETNTPFEEYLNGDEWEFRTSTLHYKPFDDEFWLHIGFKRNKEATDSTQPDAAVPEHNTVLGIDLGVENLAVASTGTFWTGDELDHWHREFQQRRGDLQQAGSQEAHRTLQRVSKKETAYYKQYLHIVANEIIEEAVEHDCSIIAFEDLTDIRKRAQGATWHHRWRFNRLYEYVEYKAKAYGIDVEELERSPQVKTVPAYYTSQRCSSCGFIHEDNRPTQEYFECQKCGYENHADYNASKNVAVRCCRRLLRRNQTGGDGGASVNIALNGGTMTVNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVJ4</accession>
    <name>A0A1U7EVJ4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1924A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322685.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVJ4"/>
    <dbReference type="STRING" id="348780.NP_1924A"/>
    <dbReference type="EnsemblBacteria" id="CAI49053">
        <property type="protein sequence ID" value="CAI49053"/>
        <property type="gene ID" value="NP_1924A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701306"/>
    <dbReference type="KEGG" id="nph:NP_1924A"/>
    <dbReference type="HOGENOM" id="CLU_1631702_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="162" mass="18848" checksum="8BBD1D007CB92DE5" modified="2017-05-10" version="1">MTTNQNKQERATYIQSVVKEEFDRDVTVRPVTRSLYEVTVGDDSIRYAKWWDRKFDELMRLTELDTDIGMPKSRVFEGDVYIQLMEPAPGRPLSHQLVLRCVPGLWWHSRDQVEAAVTQVGRYIGRLHQETSNESTEIDISNLHIDKYDAVSDGSIHPRLEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVM2</accession>
    <name>A0A1U7EVM2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1960A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVM2"/>
    <dbReference type="STRING" id="348780.NP_1960A"/>
    <dbReference type="EnsemblBacteria" id="CAI49071">
        <property type="protein sequence ID" value="CAI49071"/>
        <property type="gene ID" value="NP_1960A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_1960A"/>
    <dbReference type="HOGENOM" id="CLU_2712946_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="72" mass="7767" checksum="294211C8443B017E" modified="2017-05-10" version="1">MTGNACRCDGPPIRQTDESRHLRQQRNTGVGPIFPRLAAVIASALVAEYDPANNSPFAKLICARSQRGWASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVM6</accession>
    <name>A0A1U7EVM6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type transport system permease protein (Probable substrate urea/short-chain amides)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">urtC</name>
        <name evidence="7" type="synonym">abc10p2</name>
        <name evidence="7" type="ordered locus">NP_2000A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322720.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVM6"/>
    <dbReference type="STRING" id="348780.NP_2000A"/>
    <dbReference type="EnsemblBacteria" id="CAI49091">
        <property type="protein sequence ID" value="CAI49091"/>
        <property type="gene ID" value="NP_2000A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702400"/>
    <dbReference type="KEGG" id="nph:NP_2000A"/>
    <dbReference type="eggNOG" id="arCOG01274">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031365_0_0_2"/>
    <dbReference type="OrthoDB" id="30958at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06581">
        <property type="entry name" value="TM_PBP1_LivM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022497">
        <property type="entry name" value="ABC_trnspt_urea_permease_UrtC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043428">
        <property type="entry name" value="LivM-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03727">
        <property type="entry name" value="urea_t_UrtC_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30482:SF17">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30482">
        <property type="entry name" value="HIGH-AFFINITY BRANCHED-CHAIN AMINO ACID TRANSPORT SYSTEM PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="244"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="324"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="373" mass="39101" checksum="348698A45FC8245B" modified="2017-05-10" version="1">MSADIGSPDGPAARIRGLLEGPNTYGNSPLFWGFFVVVVAGLAVYPMTTDPFSIQTTTQYFALAFLALSLSVVWGYCGILSFGQVAFFGVAAYTFGVIGINFGTAGGITAALLGAIVVGALFAAALGYFMFYGGVRDVYVTIITLVVALVLNTFAAQTAGSEWAIGEARLGGFNGMPDIPDLVLGVGGASVAFDRVAFYYLLLAALVVTYLGLRAFVNSGFGMTMVAVREDEARTETFGYNVPFIKLVVFTIGGALAAAGGVFYAVWGNFIDPSVFGILFAALPVVWVSVGGRESLIGAIGATFAIEWMRTGLTDGVGPLGPEWAFVIIGGLLMVVILVMPAGIVPYLDRGLKQLLERINDRSTTEPTEGATE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVP0</accession>
    <name>A0A1U7EVP0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cro/C1 family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_2072A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322756.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVP0"/>
    <dbReference type="STRING" id="348780.NP_2072A"/>
    <dbReference type="EnsemblBacteria" id="CAI49127">
        <property type="protein sequence ID" value="CAI49127"/>
        <property type="gene ID" value="NP_2072A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701343"/>
    <dbReference type="KEGG" id="nph:NP_2072A"/>
    <dbReference type="eggNOG" id="arCOG01863">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1574931_0_0_2"/>
    <dbReference type="OrthoDB" id="339114at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057937">
        <property type="entry name" value="HVO_2718-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058562">
        <property type="entry name" value="MJ0586_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24250">
        <property type="entry name" value="HVO_2718"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26602">
        <property type="entry name" value="HVO_2718_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MJ0586 N-terminal zinc binding" evidence="3">
        <location>
            <begin position="15"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription regulator HVO-2718-like helix-turn-helix" evidence="2">
        <location>
            <begin position="93"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF26602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="165" mass="17807" checksum="6233F77B6E015E7C" modified="2017-05-10" version="1">MAKYSTGGVGGDESGACELCGAEDRSLETATVAGAELNVCDQCLKHGDGDAGQTDTEDRTQERKRRKKAAQNVARLDDARKVDTDWQEDTEYEEDPLPYLVSDYGTLVEQARQAEGLQIDELAREVGADEDDVVAVEQGRAARANVGGSLISALEERLDIELADT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVT2</accession>
    <name>A0A1U7EVT2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fumarylacetoacetase family protein / GNAT family acetyltransferase</fullName>
            <ecNumber evidence="4">2.3.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hpcE1</name>
        <name evidence="4" type="ordered locus">NP_2134A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAH family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49158.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVT2"/>
    <dbReference type="STRING" id="348780.NP_2134A"/>
    <dbReference type="EnsemblBacteria" id="CAI49158">
        <property type="protein sequence ID" value="CAI49158"/>
        <property type="gene ID" value="NP_2134A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_2134A"/>
    <dbReference type="eggNOG" id="arCOG00235">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG00839">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_653180_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.850.10:FF:000002">
        <property type="entry name" value="2-hydroxyhepta-2,4-diene-1,7-dioate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="entry name" value="Fumarylacetoacetase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051121">
        <property type="entry name" value="FAH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42796:SF4">
        <property type="entry name" value="FUMARYLACETOACETATE HYDROLASE DOMAIN-CONTAINING PROTEIN 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42796">
        <property type="entry name" value="FUMARYLACETOACETATE HYDROLASE DOMAIN-CONTAINING PROTEIN 2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13673">
        <property type="entry name" value="Acetyltransf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="FAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49158.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="420" mass="45437" checksum="110B97509A3D7470" modified="2017-05-10" version="1">MEIRRAVTEPALSDAFAVRRAVFVDEQGVDEDIEIDGNDGDALQFLAYDDGAAVGTARLRRPAPAIGKVERVAVREPYRGRGVGRALMEAVEAAAARLGCRELQLHGQTRVEPFYRRLDYEPEGEPFDEAGIPHLKFRKSVDGSGGDPTAAAAESPAVMAAGADGTLMRQARFEDPDGDVRYGELFEDGTVRAYPKQEKRLTAAEERFDRDDIELLAPCDPSKIVCVGLNYADHADEQGKAVPDRPLLFLKPPNTVAAPEDTLELLADRQLDYEAELGVVIGQRCRNVAEADAMDVVAGFTCVNDVSNRDDQAVETNWVRGKAFDDAAPLGPAIATPEAVPEDAAIRCRVNGDLRQDSSRDQFIFSVPELIAEITSYMTLEPGDVVSTGTPEGVGPLADGDTVEVEIEGIGTLKNDVRIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVV1</accession>
    <name>A0A1U7EVV1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Small CPxCG-related zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_2218A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVV1"/>
    <dbReference type="STRING" id="348780.NP_2218A"/>
    <dbReference type="EnsemblBacteria" id="CAI49200">
        <property type="protein sequence ID" value="CAI49200"/>
        <property type="gene ID" value="NP_2218A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_2218A"/>
    <dbReference type="eggNOG" id="arCOG04741">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_184176_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055999">
        <property type="entry name" value="DUF7577"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24463">
        <property type="entry name" value="DUF7577"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7577" evidence="3">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="91" mass="9597" checksum="47035A1055D5D6B2" modified="2017-05-10" version="1">MEGFGWLVAATVLFVAVHIVIAVYLYRKGNRKAEATAAPTDSADEPPDQVTCPTCGTPNAPGFRYCRRCVSDLSGGGTPDNKRPAAEQPGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW06</accession>
    <name>A0A1U7EW06_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7123 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2276A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322856.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW06"/>
    <dbReference type="STRING" id="348780.NP_2276A"/>
    <dbReference type="EnsemblBacteria" id="CAI49229">
        <property type="protein sequence ID" value="CAI49229"/>
        <property type="gene ID" value="NP_2276A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701398"/>
    <dbReference type="KEGG" id="nph:NP_2276A"/>
    <dbReference type="eggNOG" id="arCOG02866">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_172273_2_0_2"/>
    <dbReference type="OrthoDB" id="193681at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055547">
        <property type="entry name" value="DUF7123"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23438">
        <property type="entry name" value="DUF7123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7123" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="79" mass="9038" checksum="E701307488308B17" modified="2017-05-10" version="1">MSATTEPSADSKETRLRRYLRNRAEEGEVYIKSKFIADDVDLSPKEIGALMVKLRDSAADIEIEKWSYTSATTWRVSRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW20</accession>
    <name>A0A1U7EW20_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2374A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322904.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW20"/>
    <dbReference type="STRING" id="348780.NP_2374A"/>
    <dbReference type="EnsemblBacteria" id="CAI49278">
        <property type="protein sequence ID" value="CAI49278"/>
        <property type="gene ID" value="NP_2374A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701428"/>
    <dbReference type="KEGG" id="nph:NP_2374A"/>
    <dbReference type="eggNOG" id="arCOG10719">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_191931_0_0_2"/>
    <dbReference type="OrthoDB" id="199024at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="85" mass="9480" checksum="426F98384C706506" modified="2017-05-10" version="1">MSEAFRTVVKSNVDRDARLAAIERLVESGEKTNLRVLVQTAGLRGTFRRQALEGLVETNARAELQLLAEDRALESSLRKEAQRAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW38</accession>
    <name>A0A1U7EW38_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Bifunctional protein GlmU</fullName>
            <ecNumber evidence="3">2.3.1.157</ecNumber>
            <ecNumber evidence="4">2.7.7.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">graD5</name>
        <name evidence="14" type="ordered locus">NP_2386A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N-acetyl-alpha-D-glucosamine 1-phosphate + UTP + H(+) = UDP-N-acetyl-alpha-D-glucosamine + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13509"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="EC" id="2.7.7.23"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>alpha-D-glucosamine 1-phosphate + acetyl-CoA = N-acetyl-alpha-D-glucosamine 1-phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13725"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="ChEBI" id="CHEBI:58516"/>
            <dbReference type="EC" id="2.3.1.157"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route II): step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.157" evidence="3"/>
    <dbReference type="EC" id="2.7.7.23" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322910.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW38"/>
    <dbReference type="STRING" id="348780.NP_2386A"/>
    <dbReference type="EnsemblBacteria" id="CAI49284">
        <property type="protein sequence ID" value="CAI49284"/>
        <property type="gene ID" value="NP_2386A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702774"/>
    <dbReference type="KEGG" id="nph:NP_2386A"/>
    <dbReference type="eggNOG" id="arCOG00666">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_0_1_2"/>
    <dbReference type="OrthoDB" id="15372at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019134">
        <property type="term" value="F:glucosamine-1-phosphate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003977">
        <property type="term" value="F:UDP-N-acetylglucosamine diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd04181">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050065">
        <property type="entry name" value="GlmU-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056729">
        <property type="entry name" value="GMPPB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43584:SF8">
        <property type="entry name" value="N-ACETYLMURAMATE ALPHA-1-PHOSPHATE URIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43584">
        <property type="entry name" value="NUCLEOTIDYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25087">
        <property type="entry name" value="GMPPB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="12">
        <location>
            <begin position="6"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Mannose-1-phosphate guanyltransferase C-terminal" evidence="13">
        <location>
            <begin position="262"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF25087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAI49284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="396" mass="41421" checksum="173DEDEE202E3AC3" modified="2017-05-10" version="1">MDISTGVVLAAGEGTRLRPLTRTRPKPMLPAAGRPILEHVLDVLVDCGIERLCIVVGYRRERIQSHFGDDFRGVPITYVHQKTQLGSAHALEQAAPVVDGPVLVVNGDRVIGEPIVDDVRTAFDGTPTLAAIEHPTPSKYGAVEVSDGRLRSFYEKPAPSAGPFSHINAGVYAFDRAVFETIERMPRPSGELQLPTVVETLLENKAVHAVETAGLWVDATYPWDLLAVLRELLEAGAVAIEEREPQVWVADSARVHEAATLQPPVVVGPDAEVAAGAVVGPHVAVGRNVTVGANTTVRDSLLDSDSRADVGSILCDCVLSEAASVGPGVAVPGGPGDIRVGDDIFEQQRLGAVIADRATVGGGATVTDGALIGVGATVAAGVVLEANVADEAEVVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW67</accession>
    <name>A0A1U7EW67_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">cytochrome-c oxidase</fullName>
            <ecNumber evidence="3">7.1.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">coxA1</name>
        <name evidence="18" type="ordered locus">NP_2456A</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the heme-copper respiratory oxidase family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.9" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322945.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW67"/>
    <dbReference type="STRING" id="348780.NP_2456A"/>
    <dbReference type="EnsemblBacteria" id="CAI49319">
        <property type="protein sequence ID" value="CAI49319"/>
        <property type="gene ID" value="NP_2456A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702624"/>
    <dbReference type="KEGG" id="nph:NP_2456A"/>
    <dbReference type="eggNOG" id="arCOG01237">
        <property type="taxonomic scope" value="Archaea"/>
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</entry>
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</entry>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </dbReference>
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    </feature>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI49326.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="76" mass="7885" checksum="CB7EA805BDD74254" modified="2017-05-10" version="1">MVVAYITVKANTGEADRLRSDIKAIEGVVSAHIVAGDVDIIAKAEVESPGDVKDIAATSIQSLDGVDDTHTYVAMG</sequence>
</entry>
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    <accession>A0A1U7EWB7</accession>
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        <submittedName>
            <fullName evidence="3">DUF2062 family protein</fullName>
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    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <dbReference type="HOGENOM" id="CLU_1590937_0_0_2"/>
    <dbReference type="OrthoDB" id="329979at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018639">
        <property type="entry name" value="DUF2062"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40547">
        <property type="entry name" value="SLL0298 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40547:SF1">
        <property type="entry name" value="SLL0298 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09835">
        <property type="entry name" value="DUF2062"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2062" evidence="2">
        <location>
            <begin position="23"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="166" mass="17677" checksum="438830691DC390E5" modified="2017-05-10" version="1">MASSRLGSYRARIRGALRTALEEEHPPKEVSGSFALGTFITMLPTLGTGLVLFVVLVAIFDRISKVALLASVVVFNPVVKWGVYGASFALGVAILGPVEGVSPTSASLSAGPDIIVRLLVGNLILAVIATVISYFVVYRLAVAYRHTEVGERIEEAVHDIADELKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWF6</accession>
    <name>A0A1U7EWF6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DUF3006 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2658A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWF6"/>
    <dbReference type="STRING" id="348780.NP_2658A"/>
    <dbReference type="EnsemblBacteria" id="CAI49420">
        <property type="protein sequence ID" value="CAI49420"/>
        <property type="gene ID" value="NP_2658A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_2658A"/>
    <dbReference type="eggNOG" id="arCOG06330">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170810_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021377">
        <property type="entry name" value="DUF3006"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11213">
        <property type="entry name" value="DUF3006"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="117" mass="12684" checksum="C374DE1EEF75CF4D" modified="2017-05-10" version="1">MVYIPERGQHVSMRTATALCVVALLSTALLSGVAAAEADQTAVVDRIGDDYAVMLIEEDGETVDQAVVDPETLPDEGRYEGAIYQVVDGEYVYDGEESDDRDSRLGELLEALSERLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWI8</accession>
    <name>A0A1U7EWI8_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone 5'-phosphate reductase</fullName>
            <ecNumber evidence="9">1.1.1.302</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">arfC</name>
        <name evidence="11" type="ordered locus">NP_2672A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>2,5-diamino-6-(1-D-ribitylamino)pyrimidin-4(3H)-one 5'-phosphate + NAD(+) = 2,5-diamino-6-(1-D-ribosylamino)pyrimidin-4(3H)-one 5'-phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27274"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58890"/>
            <dbReference type="ChEBI" id="CHEBI:59545"/>
            <dbReference type="EC" id="1.1.1.302"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2,5-diamino-6-(1-D-ribitylamino)pyrimidin-4(3H)-one 5'-phosphate + NADP(+) = 2,5-diamino-6-(1-D-ribosylamino)pyrimidin-4(3H)-one 5'-phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27278"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58890"/>
            <dbReference type="ChEBI" id="CHEBI:59545"/>
            <dbReference type="EC" id="1.1.1.302"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; riboflavin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HTP reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.302" evidence="9"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323052.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWI8"/>
    <dbReference type="STRING" id="348780.NP_2672A"/>
    <dbReference type="EnsemblBacteria" id="CAI49427">
        <property type="protein sequence ID" value="CAI49427"/>
        <property type="gene ID" value="NP_2672A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703107"/>
    <dbReference type="KEGG" id="nph:NP_2672A"/>
    <dbReference type="eggNOG" id="arCOG01484">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036590_4_1_2"/>
    <dbReference type="OrthoDB" id="10178at2157"/>
    <dbReference type="UniPathway" id="UPA00275"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008703">
        <property type="term" value="F:5-amino-6-(5-phosphoribosylamino)uracil reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="entry name" value="Dihydrofolate Reductase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006401">
        <property type="entry name" value="Rib_reduct_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011549">
        <property type="entry name" value="RibD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002734">
        <property type="entry name" value="RibDG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050765">
        <property type="entry name" value="Riboflavin_Biosynth_HTPR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01508">
        <property type="entry name" value="rib_reduct_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00227">
        <property type="entry name" value="ribD_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38011:SF7">
        <property type="entry name" value="2,5-DIAMINO-6-RIBOSYLAMINO-4(3H)-PYRIMIDINONE 5'-PHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38011">
        <property type="entry name" value="DIHYDROFOLATE REDUCTASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_8G06820)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01872">
        <property type="entry name" value="RibD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="Dihydrofolate reductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0686">Riboflavin biosynthesis</keyword>
    <feature type="domain" description="Bacterial bifunctional deaminase-reductase C-terminal" evidence="10">
        <location>
            <begin position="3"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="NCBIfam" id="TIGR01508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI49427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="219" mass="23248" checksum="9BC6C138CFE72EDC" modified="2017-05-10" version="1">MDVTVNAAMSADGKLSSRRREQIAISGDADFERMYRLRAAVDAVLVGVGTVLADNPSLRSHDEAHRAEVYGPDADPPARVVADSACRTPPDADILAGEPDTYVLTSGAAPEDRRAALREAGAELIVTDGDRVSLSTAFAELAARGIDRLMVEGGGELIFSLFEAGLVDSLSVYIGDLVIGGRDAPTLADGEGFVADFPELELDEVERLDEGVVLYWEVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWK5</accession>
    <name>A0A1U7EWK5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NRDE domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2764A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323098.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWK5"/>
    <dbReference type="STRING" id="348780.NP_2764A"/>
    <dbReference type="EnsemblBacteria" id="CAI49473">
        <property type="protein sequence ID" value="CAI49473"/>
        <property type="gene ID" value="NP_2764A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701549"/>
    <dbReference type="KEGG" id="nph:NP_2764A"/>
    <dbReference type="eggNOG" id="arCOG06429">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047037_2_0_2"/>
    <dbReference type="OrthoDB" id="312503at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.60.10">
        <property type="entry name" value="Penicillin V Acylase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008551">
        <property type="entry name" value="TANGO2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17985">
        <property type="entry name" value="SER/THR-RICH PROTEIN T10 IN DGCR REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17985:SF8">
        <property type="entry name" value="TRANSPORT AND GOLGI ORGANIZATION PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05742">
        <property type="entry name" value="TANGO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="245" mass="26682" checksum="BE0A4BA8A19263D5" modified="2017-05-10" version="1">MCTLVAAWHAFDNAPLCVAANRDEAANRPSTPPFVRDRDPTVVAPRDDRAGGTWLGYNAAGVLVAVTNRWRAGDGDRSRGLLVDEALGYRSAAAAVDHVRAELEARRYAPFHLLVADAENCLLVVHDSGDTGVVHQLSPGVHAVVNVGFDGDWFTPTERPELGRQQARNTERLRSALEPKPDESAADWTSRAGTLLGDHEYGACIHRGQFGTRSSSLVRLGEQRRFEFADGPPCDAEYRPVEDAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWL1</accession>
    <name>A0A1U7EWL1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2772A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323102.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWL1"/>
    <dbReference type="STRING" id="348780.NP_2772A"/>
    <dbReference type="EnsemblBacteria" id="CAI49477">
        <property type="protein sequence ID" value="CAI49477"/>
        <property type="gene ID" value="NP_2772A"/>
    </dbReference>
    <dbReference type="GeneID" id="43500091"/>
    <dbReference type="KEGG" id="nph:NP_2772A"/>
    <dbReference type="eggNOG" id="arCOG15220">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3094199_0_0_2"/>
    <dbReference type="OrthoDB" id="225652at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="51" mass="5724" checksum="98E31A6BD6904D9E" modified="2017-05-10" version="1">MVENHTPYEYGDADIAAIGEEPPHERQHGSDMVIVDGAAMSYRSYTRAEQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWL8</accession>
    <name>A0A1U7EWL8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable transcription factor (Homolog to RNA polymerase III subunit RPC34)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rpc34</name>
        <name evidence="2" type="ordered locus">NP_2762A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323097.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWL8"/>
    <dbReference type="STRING" id="348780.NP_2762A"/>
    <dbReference type="EnsemblBacteria" id="CAI49472">
        <property type="protein sequence ID" value="CAI49472"/>
        <property type="gene ID" value="NP_2762A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701548"/>
    <dbReference type="KEGG" id="nph:NP_2762A"/>
    <dbReference type="eggNOG" id="arCOG04153">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142666_1_0_2"/>
    <dbReference type="OrthoDB" id="31046at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01047">
        <property type="entry name" value="MarR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="16"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="118" mass="13425" checksum="402C365883595707" modified="2017-05-10" version="1">MSTATDADLSEIEREGLELIRETGGIHQSDFWKELDVDSRKGSRILDSLEEEGLVEREETVYDGHNTYLVTPVRDPKDLDFSLLMAGDMLSPFVGDEEIDAQSDAFSQWVMNLAYEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWM2</accession>
    <name>A0A1U7EWM2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2790A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWM2"/>
    <dbReference type="STRING" id="348780.NP_2790A"/>
    <dbReference type="EnsemblBacteria" id="CAI49486">
        <property type="protein sequence ID" value="CAI49486"/>
        <property type="gene ID" value="NP_2790A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_2790A"/>
    <dbReference type="HOGENOM" id="CLU_2550373_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="82" mass="8753" checksum="0DE7F61B529CA4EE" modified="2017-05-10" version="1">MQRRQSPKQTQLLWGGGVAGGIGAGFLAPALHDGLYDAVFRLLYGTPDHVVHPLEQDMPILLVGFLTTIVGVVVYAVGSRIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWM9</accession>
    <name>A0A1U7EWM9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DoxX domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">NP_2808A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323120.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWM9"/>
    <dbReference type="STRING" id="348780.NP_2808A"/>
    <dbReference type="EnsemblBacteria" id="CAI49495">
        <property type="protein sequence ID" value="CAI49495"/>
        <property type="gene ID" value="NP_2808A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701565"/>
    <dbReference type="KEGG" id="nph:NP_2808A"/>
    <dbReference type="eggNOG" id="arCOG03070">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058421_8_2_2"/>
    <dbReference type="OrthoDB" id="340328at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032808">
        <property type="entry name" value="DoxX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07681">
        <property type="entry name" value="DoxX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="51"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI49495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="138" mass="14233" checksum="8EC782F64661F316" modified="2017-05-10" version="1">MFESAGADIVFVLARVLFGATLAFTGVNHFLDTDGMAEYAEFKGLPFPRASVLLSGGLLVFGGLSVVAGVVPAIGAGGLALFLLASAVTMHDFWNMDGEEAQNEMNAFLKNVFGAGGALAFLAVAGSTWPYALNIGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWS0</accession>
    <name>A0A1U7EWS0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2940A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWS0"/>
    <dbReference type="EnsemblBacteria" id="CAI49561">
        <property type="protein sequence ID" value="CAI49561"/>
        <property type="gene ID" value="NP_2940A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_2940A"/>
    <dbReference type="eggNOG" id="arCOG09387">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_216660_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="47" mass="5138" checksum="E43E7B843C85B3DA" modified="2017-05-10" version="1">MRPNIDISHTLGGRIKDYAEANGLDLSEAYTEVLEAGLEAVEHPDEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWS9</accession>
    <name>A0A1U7EWS9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CbaC protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cbaC</name>
        <name evidence="3" type="ordered locus">NP_2960A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323195.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWS9"/>
    <dbReference type="STRING" id="348780.NP_2960A"/>
    <dbReference type="EnsemblBacteria" id="CAI49571">
        <property type="protein sequence ID" value="CAI49571"/>
        <property type="gene ID" value="NP_2960A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702563"/>
    <dbReference type="KEGG" id="nph:NP_2960A"/>
    <dbReference type="eggNOG" id="arCOG10192">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_202267_0_0_2"/>
    <dbReference type="OrthoDB" id="177830at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="81" mass="8599" checksum="442B9BC99C3A5035" modified="2017-05-10" version="1">MRVSRAGLLVAIAFLVPVIVELRTALVWVGIDLTVLQTVLLALFAVAALLAWAVAPDVRGSDDDDSESPSRPETPNRTSGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWX0</accession>
    <name>A0A1U7EWX0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2990A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323210.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWX0"/>
    <dbReference type="STRING" id="348780.NP_2990A"/>
    <dbReference type="EnsemblBacteria" id="CAI49586">
        <property type="protein sequence ID" value="CAI49586"/>
        <property type="gene ID" value="NP_2990A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701618"/>
    <dbReference type="KEGG" id="nph:NP_2990A"/>
    <dbReference type="eggNOG" id="arCOG08110">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026218_0_0_2"/>
    <dbReference type="OrthoDB" id="343201at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="279"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="311"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="339"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="369"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="397"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="417" mass="45734" checksum="0B4ABF6C53C5777B" modified="2017-05-10" version="1">MDGEELDIGENTAWVAIAVVAVATLGGAVLLAEWPLWGVQPDADDEPHLVEPADGGTELWPYTSRAMDHSTRTLGINVVFHGDPGDVRTALEERSALRWEEEQVHEGDADSDTVSPDRIEVDPEADDIADIISWEPAEGSVRYSYFEVDGDGQWVDESYELHSGTYLGERLHIRAYDEPQQEWTAVQIHEEHWDWFRLRHTVTGISDSQRELERDFMDQPGVDEVVRLPFENETADSDGWVTNIYFAGAVLPFLLFGVVSRATAARRQTAQFLREHRRAIALGATLFALYTAVRWLGIAGELLFDGVSPKVVAAPLYLALAVGIPAVAYRFGRGSDTTWAFSFAVLGLGAALIVDFVAMGVSVLPLRYILHRAAVLLAVGLIAVGGALAIDDRRPAPLSAGLACWALALLLPVFGYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWY2</accession>
    <name>A0A1U7EWY2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Homolog to vitamin K epoxide reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">NP_3010A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VKOR family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323220.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWY2"/>
    <dbReference type="STRING" id="348780.NP_3010A"/>
    <dbReference type="EnsemblBacteria" id="CAI49596">
        <property type="protein sequence ID" value="CAI49596"/>
        <property type="gene ID" value="NP_3010A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701626"/>
    <dbReference type="KEGG" id="nph:NP_3010A"/>
    <dbReference type="eggNOG" id="arCOG03749">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1302654_0_0_2"/>
    <dbReference type="OrthoDB" id="200082at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10546">
        <property type="entry name" value="VKOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.130">
        <property type="entry name" value="VKOR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012932">
        <property type="entry name" value="VKOR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038354">
        <property type="entry name" value="VKOR_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34573:SF1">
        <property type="entry name" value="VITAMIN K EPOXIDE REDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34573">
        <property type="entry name" value="VKC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07884">
        <property type="entry name" value="VKOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00756">
        <property type="entry name" value="VKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0874">Quinone</keyword>
    <keyword evidence="9" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="21"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="70"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="130"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Vitamin K epoxide reductase" evidence="11">
        <location>
            <begin position="19"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI49596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="211" mass="22847" checksum="046EDC25800871CA" modified="2017-05-10" version="1">MSTETSSTIQFDYEWEYSPSVSGLLGGFTLVAVFGWLITVALTAIHFFAIPAIPADAQVQGSIEVITSQWAYVLGVPLATLGSFYYITTIGLALWWFDTRHPLIIKVLTPITASGVLFSAYFVYLQLVPIGAICPFCMMSAAATVILFGLELVVLSQSRTPSLSSMSGDIGRVLRDTNYALVVFPVLVGVATIFGLYMVTMLPLPDVVPFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX25</accession>
    <name>A0A1U7EX25_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">2-(3-amino-3-carboxypropyl)histidine synthase</fullName>
            <ecNumber evidence="3 10">2.5.1.108</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">dph2</name>
        <name evidence="11" type="ordered locus">NP_3154A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the first step of diphthamide biosynthesis, i.e. the transfer of the 3-amino-3-carboxypropyl group from S-adenosyl-L-methionine (SAM) to the C2 position of the imidazole ring of the target histidine residue in translation elongation factor 2 (EF-2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>L-histidyl-[translation elongation factor 2] + S-adenosyl-L-methionine = 2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36783"/>
            <dbReference type="Rhea" id="RHEA-COMP:9748"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:29979"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="EC" id="2.5.1.108"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DPH1/DPH2 family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.108" evidence="3 10"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323290.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX25"/>
    <dbReference type="STRING" id="348780.NP_3154A"/>
    <dbReference type="EnsemblBacteria" id="CAI49668">
        <property type="protein sequence ID" value="CAI49668"/>
        <property type="gene ID" value="NP_3154A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701672"/>
    <dbReference type="KEGG" id="nph:NP_3154A"/>
    <dbReference type="eggNOG" id="arCOG04112">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037146_0_0_2"/>
    <dbReference type="OrthoDB" id="314at2157"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090560">
        <property type="term" value="F:2-(3-amino-3-carboxypropyl)histidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11840">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11850">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11860">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016435">
        <property type="entry name" value="DPH1/DPH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042263">
        <property type="entry name" value="DPH1/DPH2_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042264">
        <property type="entry name" value="DPH1/DPH2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042265">
        <property type="entry name" value="DPH1/DPH2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035435">
        <property type="entry name" value="DPH1/DPH2_euk_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022428">
        <property type="entry name" value="Dph2_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03682">
        <property type="entry name" value="arCOG04112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00322">
        <property type="entry name" value="diphth2_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762:SF1">
        <property type="entry name" value="2-(3-AMINO-3-CARBOXYPROPYL)HISTIDINE SYNTHASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762">
        <property type="entry name" value="DIPHTHAMIDE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01866">
        <property type="entry name" value="Diphthamide_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004967">
        <property type="entry name" value="DPH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01121">
        <property type="entry name" value="Diphthamide_biosynthesis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00032">
        <property type="entry name" value="Radical_SAM_3-amino-3-carboxyp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 10" id="KW-0408">Iron</keyword>
    <keyword evidence="8 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 10 11" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR004967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI49668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="351" mass="39693" checksum="559F8456F63BDB86" modified="2017-05-10" version="1">MSQQPESDRTEGDLRNTGMELKHERTWDYELDRIVDAVEERDAMKVGLQFPEGLKRRAGRVADDLRRLLPDETTVLLSGQPCYGACDLDTYMMRRTDVFVHFGHSPMKESDKIIYVPLFSNVEVTPIMEEARAELAAPEEDDAVGLVTTAQHMNRFDEMQAWLEERGYTVETRRGDERLTHEGQVLGCNYASADVDADQILYVGGGKFHPLGLAMEHPDKKVIIADPVNNAVHVADTEQFMKQRYAAVHKAMDAEEWGVIFCTKIGQGRWDTAEEIVEANDNAYLITMDEVTPDRLTNFGFDAYVNTGCPRITTDDGPQFKQPMLTPGEYKIAIGEKPMDSLSFDTFHGTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX26</accession>
    <name>A0A1U7EX26_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF8173 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_3156A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323291.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX26"/>
    <dbReference type="STRING" id="348780.NP_3156A"/>
    <dbReference type="EnsemblBacteria" id="CAI49669">
        <property type="protein sequence ID" value="CAI49669"/>
        <property type="gene ID" value="NP_3156A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701673"/>
    <dbReference type="KEGG" id="nph:NP_3156A"/>
    <dbReference type="eggNOG" id="arCOG07873">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_932613_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058486">
        <property type="entry name" value="DUF8173"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26514">
        <property type="entry name" value="DUF8173"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="DUF8173" evidence="3">
        <location>
            <begin position="16"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="207"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="267"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF26514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="298" mass="31091" checksum="57CC6A7602EDA4B3" modified="2017-05-10" version="1">MVCPPRRFLPPAVPVAGTIAAVPTVAAQPSSEEVVAATIGDALGSMLWALIALAFWVVIAPDYSRDVTDWSIKQPGSAFLYGVAVTLGVVVVTFLLVVTVVGVLVAIPLLLAVALAVPLGFLAVGRTVTDSLGAAAIVGVVIAGVTGLVPVLGGIVGFVCSCLALGGIFLEYTEGDDGPRGRPDRPPSTGDDTPYGPLSEDPDRASDSGGHSSSPPGSRQTGGSNDGRPEKGASEDWGAGSSRSLPEESPEERRRDNPDDAPEDPPEDPREDLPEDRWNESVEDWDWGLDEDEDEDDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXG5</accession>
    <name>A0A1U7EXG5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3530A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323476.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXG5"/>
    <dbReference type="STRING" id="348780.NP_3530A"/>
    <dbReference type="EnsemblBacteria" id="CAI49856">
        <property type="protein sequence ID" value="CAI49856"/>
        <property type="gene ID" value="NP_3530A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701797"/>
    <dbReference type="KEGG" id="nph:NP_3530A"/>
    <dbReference type="HOGENOM" id="CLU_1901996_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="133" mass="14345" checksum="1090D49353AC922B" modified="2017-05-10" version="1">MQHPWTLDDDEMPTDPRAKKRTRRLRIVGLGLWGLLLGGGSVIVATGTVDLTAGEAALLGGIGVVASLVAIVVGALAIVLLRRFGILGKLIVWLLLFFALLSLLPELVGWLTENVSLFESLPVDDDQLFDLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXH5</accession>
    <name>A0A1U7EXH5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">IclR family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">NP_3608A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49895.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_148215450.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXH5"/>
    <dbReference type="EnsemblBacteria" id="CAI49895">
        <property type="protein sequence ID" value="CAI49895"/>
        <property type="gene ID" value="NP_3608A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701822"/>
    <dbReference type="KEGG" id="nph:NP_3608A"/>
    <dbReference type="eggNOG" id="arCOG02798">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062618_6_1_2"/>
    <dbReference type="OrthoDB" id="14763at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050707">
        <property type="entry name" value="HTH_MetabolicPath_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014757">
        <property type="entry name" value="Tscrpt_reg_IclR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005471">
        <property type="entry name" value="Tscrpt_reg_IclR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30136">
        <property type="entry name" value="HELIX-TURN-HELIX TRANSCRIPTIONAL REGULATOR, ICLR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30136:SF35">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR RV1719"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09339">
        <property type="entry name" value="HTH_IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01614">
        <property type="entry name" value="IclR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00346">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51077">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51078">
        <property type="entry name" value="ICLR_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH iclR-type" evidence="4">
        <location>
            <begin position="6"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="IclR-ED" evidence="5">
        <location>
            <begin position="67"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI49895.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="250" mass="27143" checksum="B3446AF8F3ED54DF" modified="2017-05-10" version="1">MSDDRVQATATSVRILEGIVELDGEAGVTELSTHLSLAKSTVYKHLNTLQTTGVVVQRGDRYRIGLKTLEFGGYAQRHDGIYETARPEIESMARKSGELANLMFEEGGRGVYVHTARGDDAVDLDTQTGRRVHLHATGLGKAILATLDDERVRTIIDRHGLPEVTPHTITDEEALFEELAAIRSAGVAYDREECVPGMACIARPLSTPGPRPAAISITGPVSRITADETESEARQLVEQTGNVIELNLTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXI3</accession>
    <name>A0A1U7EXI3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type transport system ATP-binding protein (Probable substrate dipeptide/oligopeptide)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">dppDF2</name>
        <name evidence="7" type="synonym">dppD3a:abc02a</name>
        <name evidence="7" type="ordered locus">NP_3584A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323503.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXI3"/>
    <dbReference type="STRING" id="348780.NP_3584A"/>
    <dbReference type="EnsemblBacteria" id="CAI49883">
        <property type="protein sequence ID" value="CAI49883"/>
        <property type="gene ID" value="NP_3584A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702367"/>
    <dbReference type="KEGG" id="nph:NP_3584A"/>
    <dbReference type="eggNOG" id="arCOG00185">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_86_2_2"/>
    <dbReference type="OrthoDB" id="18209at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03257">
        <property type="entry name" value="ABC_NikE_OppD_transporters"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050319">
        <property type="entry name" value="ABC_transp_ATP-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007739">
        <property type="entry name" value="PRK10419.1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43776:SF7">
        <property type="entry name" value="D,D-DIPEPTIDE TRANSPORT ATP-BINDING PROTEIN DDPF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43776">
        <property type="entry name" value="TRANSPORT ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="11"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="329"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="288"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="588"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="588"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="616" mass="65447" checksum="97D12730C4D8718F" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXJ1</accession>
    <name>A0A1U7EXJ1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Translation initiation factor aIF-1 (SUI1 protein, bacterial-type IF3)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">tif1b</name>
        <name evidence="6" type="synonym">sui1b</name>
        <name evidence="6" type="ordered locus">NP_3572A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SUI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49877.3"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_394296333.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXJ1"/>
    <dbReference type="STRING" id="348780.NP_3572A"/>
    <dbReference type="EnsemblBacteria" id="CAI49877">
        <property type="protein sequence ID" value="CAI49877"/>
        <property type="gene ID" value="NP_3572A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703264"/>
    <dbReference type="KEGG" id="nph:NP_3572A"/>
    <dbReference type="eggNOG" id="arCOG04223">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082805_6_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002188">
        <property type="term" value="P:translation reinitiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11567">
        <property type="entry name" value="YciH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050318">
        <property type="entry name" value="DENR/SUI1_TIF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005872">
        <property type="entry name" value="SUI1_arc_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12789:SF0">
        <property type="entry name" value="DENSITY-REGULATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12789">
        <property type="entry name" value="DENSITY-REGULATED PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="eIF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="SUI1" evidence="5">
        <location>
            <begin position="27"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI49877.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="98" mass="10573" checksum="52F086EC081CC9ED" modified="2017-05-10" version="1">MTDDDSMEDLLAELDSHSDLSKAGQALRIRTEKRQYGKAVTIIEGFDTTATDVQSLASDLKSELATGGTSASDSIELQGDHTGRLPSLLEERGFEIVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXK5</accession>
    <name>A0A1U7EXK5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC-type transport system accessory transmembrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">abc24u</name>
        <name evidence="2" type="ordered locus">NP_3646A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323532.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXK5"/>
    <dbReference type="STRING" id="348780.NP_3646A"/>
    <dbReference type="EnsemblBacteria" id="CAI49914">
        <property type="protein sequence ID" value="CAI49914"/>
        <property type="gene ID" value="NP_3646A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702442"/>
    <dbReference type="KEGG" id="nph:NP_3646A"/>
    <dbReference type="eggNOG" id="arCOG06625">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_152361_2_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008407">
        <property type="entry name" value="Brnchd-chn_aa_trnsp_AzlD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05437">
        <property type="entry name" value="AzlD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="100" mass="9933" checksum="269A79E701DD19C1" modified="2017-05-10" version="1">MLELGTLTVGVILGMAVLTYITKAGGLWLLGHVDIGDRTEAALEALPGAIIIAIVAPELASGGPAEWAAAAVAAGVAIKTDNLLISLAAAMGALVVFRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXS5</accession>
    <name>A0A1U7EXS5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3850A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXS5"/>
    <dbReference type="STRING" id="348780.NP_3850A"/>
    <dbReference type="EnsemblBacteria" id="CAI50016">
        <property type="protein sequence ID" value="CAI50016"/>
        <property type="gene ID" value="NP_3850A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3850A"/>
    <dbReference type="eggNOG" id="arCOG09009">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_718904_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="384" mass="43596" checksum="C7070DC3CA9B48E0" modified="2017-05-10" version="1">MSVHLNKYEQKKPTQAEQTSRHQRRNRSNHRLHQSCGRYSTPLPVSLSRRATRNVAHPRSQRGSNLANCRLRAHPTAQSRTDRSRSGVPGTASDRLMSRSKQPLEDVPSLPTIRSEPENDPQPYGDRASVLAATDGTSIFESTPTVVLCPNCGDVVATYEQGLPQIVPQFDSRCTTCQVELRRWCAVAVDAAYKELVEPPTLTAMTQAYWNEWLWAGITNYKGQPRNDEYTNRLSAKASEFGWDWELTCPLCRRGISQLKQDVSGVNGSLDYHHWSTDPDQGITLCRECHDVIGFDSYDNQVEERAHEWGFRSRNDLQIVRLALREAIATDRTIELDMAERLVNRYNLIQSTDEVQELLQTVLCDEDLYNLFVDASLNNGLDNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXS9</accession>
    <name>A0A1U7EXS9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DMT superfamily transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tp25</name>
        <name evidence="7" type="ordered locus">NP_3806A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323611.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXS9"/>
    <dbReference type="STRING" id="348780.NP_3806A"/>
    <dbReference type="EnsemblBacteria" id="CAI49994">
        <property type="protein sequence ID" value="CAI49994"/>
        <property type="gene ID" value="NP_3806A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703301"/>
    <dbReference type="KEGG" id="nph:NP_3806A"/>
    <dbReference type="eggNOG" id="arCOG00271">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033863_5_1_2"/>
    <dbReference type="OrthoDB" id="17861at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050638">
        <property type="entry name" value="AA-Vitamin_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32322:SF2">
        <property type="entry name" value="EAMA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32322">
        <property type="entry name" value="INNER MEMBRANE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="217"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="250"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="273"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="6">
        <location>
            <begin position="6"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="6">
        <location>
            <begin position="155"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="316" mass="31891" checksum="C6CFF9D3E18BF966" modified="2017-05-10" version="1">MSLRRAIGLFAVASLAFGGTFVAARAGQVYIPPLLFVAIRFDLAAILLLGYAAYATEGSIRPRTRGDIGGVLAAGVLTIGLANGLLFVGQGYVTSAVGAILFSLVPIIAPLFAALLLSDERVSALGAVGMGVAAIGVGMVVGVSPSNLLAESGVGAALVLGGAASAALGTVLTRRCEATISSTAQVAWGLPISAVLLHGMSLAVGESAAAIEWTLTAIVSLAYVTVVAGAVGYIAYFALIEEVGALRSSLIFYASPLVAALGGWWFLGEAITTTTVAGFVTVVAGFLVIGHEQFRAAFRRLADGRGQATGGYEPSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXU9</accession>
    <name>A0A1U7EXU9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3844A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323630.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXU9"/>
    <dbReference type="STRING" id="348780.NP_3844A"/>
    <dbReference type="EnsemblBacteria" id="CAI50013">
        <property type="protein sequence ID" value="CAI50013"/>
        <property type="gene ID" value="NP_3844A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701883"/>
    <dbReference type="KEGG" id="nph:NP_3844A"/>
    <dbReference type="eggNOG" id="arCOG14593">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2152682_0_0_2"/>
    <dbReference type="OrthoDB" id="342934at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="111" mass="11117" checksum="80CD208EFF796A3A" modified="2017-05-10" version="1">MDVDWQNSGGIVALGLLGIALVLTLAGIETVAGYEVLSAGQSAFGFLAAGNFSLGVLSAGIFSVGIFSAGVFSVGVFSIGIFAVGPFAFGIYAIGFYATQKYVASSETTTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXV6</accession>
    <name>A0A1U7EXV6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Probable ADP-ribosylglycohydrolase</fullName>
            <ecNumber evidence="4">3.2.2.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_3878A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="3">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ADP-ribosylglycohydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.-" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323647.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXV6"/>
    <dbReference type="STRING" id="348780.NP_3878A"/>
    <dbReference type="EnsemblBacteria" id="CAI50030">
        <property type="protein sequence ID" value="CAI50030"/>
        <property type="gene ID" value="NP_3878A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701899"/>
    <dbReference type="KEGG" id="nph:NP_3878A"/>
    <dbReference type="eggNOG" id="arCOG04448">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024566_8_2_2"/>
    <dbReference type="OrthoDB" id="114878at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4080.10">
        <property type="entry name" value="ADP-ribosylation/Crystallin J1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050792">
        <property type="entry name" value="ADP-ribosylglycohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005502">
        <property type="entry name" value="Ribosyl_crysJ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036705">
        <property type="entry name" value="Ribosyl_crysJ1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16222">
        <property type="entry name" value="ADP-RIBOSYLGLYCOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16222:SF24">
        <property type="entry name" value="ADP-RIBOSYLHYDROLASE ARH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03747">
        <property type="entry name" value="ADP_ribosyl_GH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101478">
        <property type="entry name" value="ADP-ribosylglycohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR605502-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="305" mass="32874" checksum="0677063CE88BAC45" modified="2017-05-10" version="1">MSTVDRAPGILLGLACGDALGRPVEFRSATQIKREFGQLDEMIGNGTHKQPAGTITDDTELALRIARSLVEQKEFDGADVAERFVEWYRSGPFDIGLMTADALQRIENGMLWDEAGQEVWEERPEGQNAGNGSVMRCAPYSIAFSEDPDQLLSVSKASSRITHADPRCTYGCAVLNLTLAGLLHGEDEPLRRALNRVRSEAPEELVEALDPVAKGASIDTLRNSGYVIHSLQTALHDAFAADSAEEAIVTAVNRGGDTDTIGAITGAVAGARFGAGGLPDRWLETIDEREELIDLALELHEISTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXX6</accession>
    <name>A0A1U7EXX6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC-type transport system permease protein (Probable substrate copper)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">nosY1</name>
        <name evidence="2" type="synonym">abc11p</name>
        <name evidence="2" type="ordered locus">NP_3920A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323668.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXX6"/>
    <dbReference type="STRING" id="348780.NP_3920A"/>
    <dbReference type="EnsemblBacteria" id="CAI50051">
        <property type="protein sequence ID" value="CAI50051"/>
        <property type="gene ID" value="NP_3920A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702403"/>
    <dbReference type="KEGG" id="nph:NP_3920A"/>
    <dbReference type="eggNOG" id="arCOG02436">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_923218_0_0_2"/>
    <dbReference type="OrthoDB" id="313530at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12679">
        <property type="entry name" value="ABC2_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="241"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="291" mass="29775" checksum="F131373FEBF00BAB" modified="2017-05-10" version="1">MSRHRAFGAVAWRELQTVVRTRTYALLSVGLAVVFVQLLRSGGGVEMGYVPAAVDLLLALELLVPLVAVALGYRAFTGDNDDLSVLRTYPVSRSSLVAGVFVGRFLGLAAIVGAPLLLVAGMVARAGGPTSRVFATHRGVDSPVLFIRFIALTLLFGAVVLAMAMAVSVIVRSGRAALVLALAVLVVVVAGGDLLALAGLSAGIIGDDMLASVLGASPNSAYRGLVLDLVVGVATDQAGAIAVPAAVAGLATWLGGSLLIVAAGLHRQPASRLRNAIRSAIERARTTIVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY25</accession>
    <name>A0A1U7EY25_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Major facilitator superfamily transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">tp21</name>
        <name evidence="3" type="ordered locus">NP_4030A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY25"/>
    <dbReference type="STRING" id="348780.NP_4030A"/>
    <dbReference type="EnsemblBacteria" id="CAI50106">
        <property type="protein sequence ID" value="CAI50106"/>
        <property type="gene ID" value="NP_4030A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703297"/>
    <dbReference type="KEGG" id="nph:NP_4030A"/>
    <dbReference type="eggNOG" id="arCOG00131">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052485_1_0_2"/>
    <dbReference type="OrthoDB" id="117970at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43129">
        <property type="entry name" value="FOSMIDOMYCIN RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43129:SF1">
        <property type="entry name" value="FOSMIDOMYCIN RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="229"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="272"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="323"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="361"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="389"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="2">
        <location>
            <begin position="8"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="415" mass="41724" checksum="1934B5FB1EF155A0" modified="2017-05-10" version="1">MTSREATSVIGLVSGSHVVNHAYLLLFAPAFPLLSADLDVSLAALGVALGVVNVVVTVFQLPLGYVSDNYSHGAVLVGSLAVGTVGCALAAVAPDYWWLLGAAAVMGLGVAGHHPAHYPLISAATPDGFRSRAYSAHGFAGALGLALPFAVVPASVAVGGGWRLAFGAIAALGAVYTLVAVAAVRRLPESVTRAGALANDGAMDRLPAETRRERLRRVIGRVRGYGRTLLAAPLVLLLAALWFVNSVAAWGVRAYAATLLEGSYGLAPEPASLVASAMLVVGAVVLLGGGYVADKIGPMPMLFVGYGSLVVLATLVSSALLPVAAAVGVVLLLAATIDISRPARSTLTDLASERKDVGKNFAVITLGVSAGGAVAPPAFGYVIDAANTGVAFLVVAAVAALALALSVAVGRLAGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY36</accession>
    <name>A0A1U7EY36_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7122 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_4070A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323742.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY36"/>
    <dbReference type="STRING" id="348780.NP_4070A"/>
    <dbReference type="EnsemblBacteria" id="CAI50126">
        <property type="protein sequence ID" value="CAI50126"/>
        <property type="gene ID" value="NP_4070A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701959"/>
    <dbReference type="KEGG" id="nph:NP_4070A"/>
    <dbReference type="eggNOG" id="arCOG02286">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135996_0_0_2"/>
    <dbReference type="OrthoDB" id="50259at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055546">
        <property type="entry name" value="DUF7122"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23437">
        <property type="entry name" value="DUF7122"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7122" evidence="2">
        <location>
            <begin position="3"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="168" mass="18907" checksum="45081A88C39E13B1" modified="2017-05-10" version="1">MSAENDGQRFDRLPETADERTVEGRATRAEVIDWWTERFGLAADHFSDHTFWEKGAGKIWAFADDIDSPARVEGLGMTFLRTRQEHWKPTTDAVQRFGADATRNIVVLDAADAQAFLRGETTTPGWDGDWGYLIAAHEIAGAVEPIGVGLYVHDEFRSQMPKGRQREL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYB7</accession>
    <name>A0A1U7EYB7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">8-amino-7-oxononanoate synthase</fullName>
            <ecNumber evidence="5">2.3.1.47</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">7-keto-8-amino-pelargonic acid synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">8-amino-7-ketopelargonate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">bioF</name>
        <name evidence="14" type="ordered locus">NP_4232A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>6-carboxyhexanoyl-[ACP] + L-alanine + H(+) = (8S)-8-amino-7-oxononanoate + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:42288"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9955"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78846"/>
            <dbReference type="ChEBI" id="CHEBI:149468"/>
            <dbReference type="EC" id="2.3.1.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; biotin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.47" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323823.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYB7"/>
    <dbReference type="STRING" id="348780.NP_4232A"/>
    <dbReference type="EnsemblBacteria" id="CAI50207">
        <property type="protein sequence ID" value="CAI50207"/>
        <property type="gene ID" value="NP_4232A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702553"/>
    <dbReference type="KEGG" id="nph:NP_4232A"/>
    <dbReference type="eggNOG" id="arCOG00113">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015846_11_0_2"/>
    <dbReference type="OrthoDB" id="9071at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008710">
        <property type="term" value="F:8-amino-7-oxononanoate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050087">
        <property type="entry name" value="AON_synthase_class-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693:SF100">
        <property type="entry name" value="8-AMINO-7-OXONONANOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693">
        <property type="entry name" value="CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0093">Biotin biosynthesis</keyword>
    <keyword evidence="8 12" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="13">
        <location>
            <begin position="53"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAI50207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="399" mass="41568" checksum="84B55BA2B3AA0FB6" modified="2017-05-10" version="1">MQRYDTRGFDLEARLADREDTGLRRDLAPVDSVGASARTAVDPKGEPPAFGDEKLVFAANNYLGLAGDDRLAAAAADTASAVGTGAGASRLVVGDTPAHRSLERRLAAEKSAERALVFSSGYAANVGTLTALSPDRIFSDELNHASIIDGARLSGADIEVYDHCDAAALEACMRDCADEAAADESWLVVTDSVFSMDGDVAPLADICDAADTYGAWVMVDEAHATGVYDGGIVGQRGLEDRVDVQLGTLSKALGAQGGFVAGDEPLVEYLLNAARSFVFSTGLAPPAAAAAERGLALAPQQRKPLWSNVDRLRSGLEALGYEVWGDTHILPVVVGDRGDALALADRLEDAGIVAPAIRPPTVPEGTSRIRLAPQATHTAADIDRCLAAFEVAGTEVGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYF6</accession>
    <name>A0A1U7EYF6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0212 protein NP_4318A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_4318A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0212 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323866.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYF6"/>
    <dbReference type="STRING" id="348780.NP_4318A"/>
    <dbReference type="EnsemblBacteria" id="CAI50250">
        <property type="protein sequence ID" value="CAI50250"/>
        <property type="gene ID" value="NP_4318A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702029"/>
    <dbReference type="KEGG" id="nph:NP_4318A"/>
    <dbReference type="eggNOG" id="arCOG02119">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_138334_0_0_2"/>
    <dbReference type="OrthoDB" id="214737at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01223">
        <property type="entry name" value="UPF0212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007564">
        <property type="entry name" value="UPF0212"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003035">
        <property type="entry name" value="PRK03922.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42199">
        <property type="entry name" value="UPF0212 PROTEIN MJ0068"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42199:SF1">
        <property type="entry name" value="UPF0212 PROTEIN TK1194"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04475">
        <property type="entry name" value="DUF555"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="111"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="111"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="147"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="159"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="173" mass="18846" checksum="EE268F41CC84DEE8" modified="2017-05-10" version="1">MNCRVVVEAAVPVYDVETTDEAVRIAISKTGEMLNPDLNYVEIDMGSRSCAECGADQDPAFIAADEGLVALELEMTVFNVEREEHASRIARKEIGQRLENIPLEVIEVETVEEEDLDETAADDEAAVEDDADEPPAADDGATAGADGEDRTDGGRRTDDDDEVLPEFEELIDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYH4</accession>
    <name>A0A1U7EYH4_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7 8">6,7-dimethyl-8-ribityllumazine synthase</fullName>
            <shortName evidence="7">DMRL synthase</shortName>
            <shortName evidence="7">LS</shortName>
            <shortName evidence="7">Lumazine synthase</shortName>
            <ecNumber evidence="3 7 8">2.5.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">ribH</name>
        <name evidence="9" type="synonym">ribE</name>
        <name evidence="9" type="ordered locus">NP_4412A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(2S)-2-hydroxy-3-oxobutyl phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:26152"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58201"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="2.5.1.78"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the DMRL synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.78" evidence="3 7 8"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50297.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939672.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYH4"/>
    <dbReference type="STRING" id="348780.NP_4412A"/>
    <dbReference type="EnsemblBacteria" id="CAI50297">
        <property type="protein sequence ID" value="CAI50297"/>
        <property type="gene ID" value="NP_4412A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703106"/>
    <dbReference type="KEGG" id="nph:NP_4412A"/>
    <dbReference type="eggNOG" id="arCOG01323">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089358_3_0_2"/>
    <dbReference type="OrthoDB" id="7610at2157"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00404"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009349">
        <property type="term" value="C:riboflavin synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000906">
        <property type="term" value="F:6,7-dimethyl-8-ribityllumazine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.960">
        <property type="entry name" value="Lumazine/riboflavin synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00178">
        <property type="entry name" value="Lumazine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034964">
        <property type="entry name" value="LS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002180">
        <property type="entry name" value="LS/RS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036467">
        <property type="entry name" value="LS/RS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00114">
        <property type="entry name" value="lumazine-synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21058:SF0">
        <property type="entry name" value="6,7-DIMETHYL-8-RIBITYLLUMAZINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21058">
        <property type="entry name" value="6,7-DIMETHYL-8-RIBITYLLUMAZINE SYNTHASE DMRL SYNTHASE LUMAZINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00885">
        <property type="entry name" value="DMRL_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52121">
        <property type="entry name" value="Lumazine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="5 7 9" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="45"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="69"/>
            <end position="71"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="74"/>
            <end position="75"/>
        </location>
        <ligand>
            <name>(2S)-2-hydroxy-3-oxobutyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>(2S)-2-hydroxy-3-oxobutyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="NCBIfam" id="TIGR00114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI50297.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="138" mass="14532" checksum="E7EB8EAF62312218" modified="2017-05-10" version="1">MVALGLVVAQFNKERPITHEMEDRAREAAADADAEIVETIEVPGSYDTPLAADRLARRDDIDAVAVLGAIVTGDTNHDQVIADAAAQGLTDVSLDRDTPVTFGITGPGMSHAEAEERIEHGGTAVRSAISLAEELEGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYN7</accession>
    <name>A0A1U7EYN7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">precorrin-2 dehydrogenase</fullName>
            <ecNumber evidence="2">1.3.1.76</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">sirC</name>
        <name evidence="8" type="ordered locus">NP_4500A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>precorrin-2 + NAD(+) = sirohydrochlorin + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15613"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58351"/>
            <dbReference type="ChEBI" id="CHEBI:58827"/>
            <dbReference type="EC" id="1.3.1.76"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.76" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323956.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYN7"/>
    <dbReference type="STRING" id="348780.NP_4500A"/>
    <dbReference type="EnsemblBacteria" id="CAI50341">
        <property type="protein sequence ID" value="CAI50341"/>
        <property type="gene ID" value="NP_4500A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702652"/>
    <dbReference type="KEGG" id="nph:NP_4500A"/>
    <dbReference type="eggNOG" id="arCOG01044">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011276_8_1_2"/>
    <dbReference type="OrthoDB" id="10510at2157"/>
    <dbReference type="UniPathway" id="UPA00262">
        <property type="reaction ID" value="UER00222"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004325">
        <property type="term" value="F:ferrochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043115">
        <property type="term" value="F:precorrin-2 dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019354">
        <property type="term" value="P:siroheme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.110">
        <property type="entry name" value="Siroheme synthase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028161">
        <property type="entry name" value="Met8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028281">
        <property type="entry name" value="Sirohaem_synthase_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006367">
        <property type="entry name" value="Sirohaem_synthase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01470">
        <property type="entry name" value="cysG_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35330">
        <property type="entry name" value="SIROHEME BIOSYNTHESIS PROTEIN MET8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35330:SF1">
        <property type="entry name" value="SIROHEME BIOSYNTHESIS PROTEIN MET8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13241">
        <property type="entry name" value="NAD_binding_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14824">
        <property type="entry name" value="Sirohm_synth_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75615">
        <property type="entry name" value="Siroheme synthase middle domains-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Siroheme synthase central" evidence="7">
        <location>
            <begin position="120"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI50341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="215" mass="22658" checksum="54072B336536E1A4" modified="2017-05-10" version="1">MIPLYHDFTGQTVLVFGGGSVGARKARRFAREADVVVVSPAFPEADYGGASLVRAAPEPEAVDDWFDRTEPALAVAATDDAAVNEAVAEAATARGRLVNRTDRSGGRDPGSVVVPATVRDGDVTVAISTGGQSPALARELRKRIESEIEGAGELATTTASLREQLKDRDISPAARRQAVRDVVQSPQVWKDLGTGVSKHRQTVDAVVESALGDTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYR6</accession>
    <name>A0A1U7EYR6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Homolog to sulfate adenylyltransferase small subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_4570A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323991.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYR6"/>
    <dbReference type="STRING" id="348780.NP_4570A"/>
    <dbReference type="EnsemblBacteria" id="CAI50376">
        <property type="protein sequence ID" value="CAI50376"/>
        <property type="gene ID" value="NP_4570A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702650"/>
    <dbReference type="KEGG" id="nph:NP_4570A"/>
    <dbReference type="eggNOG" id="arCOG04586">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043026_1_0_2"/>
    <dbReference type="OrthoDB" id="14887at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002500">
        <property type="entry name" value="PAPS_reduct_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050128">
        <property type="entry name" value="Sulfate_adenylyltrnsfr_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43196">
        <property type="entry name" value="SULFATE ADENYLYLTRANSFERASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43196:SF1">
        <property type="entry name" value="SULFATE ADENYLYLTRANSFERASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01507">
        <property type="entry name" value="PAPS_reduct"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoadenosine phosphosulphate reductase" evidence="2">
        <location>
            <begin position="47"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoadenosine phosphosulphate reductase" evidence="2">
        <location>
            <begin position="162"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="299"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="322" mass="37152" checksum="046E015BDE8FF72F" modified="2017-05-10" version="1">MSDFPDYVDVDYTDGEGETPETYPALEDKIEKAVEVVRAGLEEYETPAIMWTGGKDSTLTLYFVKEVAEEFGYDLPPAVFIDHYQHFDELLEFVDRWADEWDLDVVFARNEDIGDYVDAHGLAPGDEIPVDELDENNRRHIREILEYEEDTFPFLLDTYVGNHLLKTVALNNALEAHDIDGIISGIRWDEQEARADETFFSPRHDPDIYPPHDRIQPILQFDEGDVWDAFWYYVVPETVEAFPDDGYVPQSYDDLPNDLGHEDIPVSPKYFAGFRSLGSEISTEKSDEEPAWLQDLENTTERAGRAQDKEDLMARLRDLGYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ10</accession>
    <name>A0A1U7EZ10_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">IS1341-type transposase ISNph5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_4810A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324108.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ10"/>
    <dbReference type="STRING" id="348780.NP_4810A"/>
    <dbReference type="EnsemblBacteria" id="CAI50496">
        <property type="protein sequence ID" value="CAI50496"/>
        <property type="gene ID" value="NP_4810A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702184"/>
    <dbReference type="KEGG" id="nph:NP_4810A"/>
    <dbReference type="eggNOG" id="arCOG00682">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055574_0_0_2"/>
    <dbReference type="OrthoDB" id="168528at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF21">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas12f1-like TNB" evidence="3">
        <location>
            <begin position="297"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="372"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="394"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="418" mass="47409" checksum="3640929397B6AA7F" modified="2017-05-10" version="1">MTATTTKTLEATLAPPTAHKERKLCDLLDTYRAGLHEAFEAGCETMTATSDVVTPYDLPYQAKAALCNYVPQLHGTYDAQELDDDHPVRLTNQAAEFDHSPERDYEFTWWVPQPGRGTNFWIPLRINPAQEELWHDIVDGEASAGQLRLQRHRTSWTLHVTVEFPVKEPGYEPTDDDVTPVGFDIGEAHLLAGCACEQGTPTDPLLINGGRARHLRKEMHTTLKRLQERDAAEWRIDERFDHYQNALTDIIEKASRQAIEYACRFEKPVVVLEDLSYIREDLDYGEWMNRRLHAWAFARLQERIEDKARESGIPVEYIRPEYTSQTCHECGHIGYRDGDEFRCQNDECWVSEYHADINAAVNIADRHDPWGESLPLKPAGDDISRDGSACDSAATPTEQSQPRQMTLGEVGSEPTAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ14</accession>
    <name>A0A1U7EZ14_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribonuclease P protein component 1</fullName>
            <shortName evidence="6">RNase P component 1</shortName>
            <ecNumber evidence="6">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Rpp29</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">rnp1</name>
        <name evidence="7" type="ordered locus">NP_4870A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Consists of a catalytic RNA component and at least 4-5 protein subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the eukaryotic/archaeal RNase P protein component 1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324138.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ14"/>
    <dbReference type="STRING" id="348780.NP_4870A"/>
    <dbReference type="EnsemblBacteria" id="CAI50526">
        <property type="protein sequence ID" value="CAI50526"/>
        <property type="gene ID" value="NP_4870A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703112"/>
    <dbReference type="KEGG" id="nph:NP_4870A"/>
    <dbReference type="eggNOG" id="arCOG00784">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107020_0_0_2"/>
    <dbReference type="OrthoDB" id="39019at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.210">
        <property type="entry name" value="Ribonuclease P/MRP, subunit p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00754">
        <property type="entry name" value="RNase_P_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036980">
        <property type="entry name" value="RNase_P/MRP_Rpp29_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023538">
        <property type="entry name" value="RNP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023534">
        <property type="entry name" value="Rof/RNase_P-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002730">
        <property type="entry name" value="Rpp29/RNP1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003020">
        <property type="entry name" value="PRK03879.2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01868">
        <property type="entry name" value="RNase_P-MRP_p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00538">
        <property type="entry name" value="POP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101744">
        <property type="entry name" value="Rof/RNase P subunit-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5 6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI50526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="98" mass="10564" checksum="49902B7D04AFE7BA" modified="2017-05-10" version="1">MNPETLVRHELVGLDVEVTDAPNPDLVGIAGRVHDETMRTLLVATGDGVKQVPKAETTFRFELDEAADNGSVAVVVEGERLVARPALRTETKGGSLWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ54</accession>
    <name>A0A1U7EZ54_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PRC domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4984A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324194.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="CAI50583">
        <property type="protein sequence ID" value="CAI50583"/>
        <property type="gene ID" value="NP_4984A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702221"/>
    <dbReference type="KEGG" id="nph:NP_4984A"/>
    <dbReference type="eggNOG" id="arCOG02155">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_174517_1_0_2"/>
    <dbReference type="OrthoDB" id="85079at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.240">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38137">
        <property type="entry name" value="PRC-BARREL DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38137:SF2">
        <property type="entry name" value="PRC-BARREL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRC-barrel" evidence="1">
        <location>
            <begin position="3"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="79" mass="8669" checksum="CC1D196BD0CEB9F5" modified="2017-05-10" version="1">MVDILAENLSEKEVMGSDGASLGTLYNITMDLKTGTLQDLVIEPGERAGDLEFERDEQGNYRIPVSRVQAVKDTVVVAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ61</accession>
    <name>A0A1U7EZ61_NATPD</name>
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        <submittedName>
            <fullName evidence="2">TorD family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4956A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324181.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ61"/>
    <dbReference type="STRING" id="348780.NP_4956A"/>
    <dbReference type="EnsemblBacteria" id="CAI50569">
        <property type="protein sequence ID" value="CAI50569"/>
        <property type="gene ID" value="NP_4956A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702212"/>
    <dbReference type="KEGG" id="nph:NP_4956A"/>
    <dbReference type="eggNOG" id="arCOG01506">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1168598_0_0_2"/>
    <dbReference type="OrthoDB" id="320758at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3480.10">
        <property type="entry name" value="TorD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020945">
        <property type="entry name" value="DMSO/NO3_reduct_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036411">
        <property type="entry name" value="TorD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050289">
        <property type="entry name" value="TorD/DmsD_chaperones"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34227">
        <property type="entry name" value="CHAPERONE PROTEIN YCDY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34227:SF1">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE CHAPERONE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02613">
        <property type="entry name" value="Nitrate_red_del"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89155">
        <property type="entry name" value="TorD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="213" mass="23408" checksum="3E27B192AB8CE6E1" modified="2017-05-10" version="1">MTQPADTAADLADLYAFLSGVLADPPSEAAVDRLASEQLPTDASPQALANGYRLLSKWQSTVSDPAEEADRLKRVHTRLFVGPRPKMQAHESWYADDYLGEPLAAVKQTYRDLGIEHSEHLREEPDHVAVELAALEMLARDGADDYRRAFLSAHGWWLPAAAADIKELTDDTFYEAVGWLAEGAVEADAYLLGLELDDLAPSYLDTGTPRTEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ85</accession>
    <name>A0A1U7EZ85_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4980A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50581.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939736.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ85"/>
    <dbReference type="EnsemblBacteria" id="CAI50581">
        <property type="protein sequence ID" value="CAI50581"/>
        <property type="gene ID" value="NP_4980A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702220"/>
    <dbReference type="KEGG" id="nph:NP_4980A"/>
    <dbReference type="HOGENOM" id="CLU_2056002_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50581.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="112" mass="11116" checksum="3C19CAD0A71DF1BF" modified="2017-05-10" version="1">MTDTETDRVAAVRRTGFGLVAGGIIFAALSTVAPGVLLAGVLLAAAVVIRFEQPPGAGVINLSAALAILGVLLFLNGALDIGPFGPAAVAGFLVVGGAFDIIAARRLAAYVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ86</accession>
    <name>A0A1U7EZ86_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4994A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324199.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ86"/>
    <dbReference type="STRING" id="348780.NP_4994A"/>
    <dbReference type="EnsemblBacteria" id="CAI50588">
        <property type="protein sequence ID" value="CAI50588"/>
        <property type="gene ID" value="NP_4994A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702225"/>
    <dbReference type="KEGG" id="nph:NP_4994A"/>
    <dbReference type="eggNOG" id="arCOG04760">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1665441_0_0_2"/>
    <dbReference type="OrthoDB" id="203616at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043850">
        <property type="entry name" value="DUF5812"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19129">
        <property type="entry name" value="DUF5812"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="154" mass="16767" checksum="360AF8EDFAFA9066" modified="2017-05-10" version="1">MNAQEGCFLVTHAEEASAVLKDVDRGQVHTLADNPGVEVRDVIEGTVAPEPPMEVAYQLVDIETRRQLSIEESPEPPTQQERAMAAEQPTGEMATEERAGTGEIHVLSVPEDQTEPAVEDVLDDEATLVRAAKLGVERVEIRSEPGVVSVRYLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZE4</accession>
    <name>A0A1U7EZE4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Probable secreted glycoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_5184A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50683.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324293.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZE4"/>
    <dbReference type="STRING" id="348780.NP_5184A"/>
    <dbReference type="EnsemblBacteria" id="CAI50683">
        <property type="protein sequence ID" value="CAI50683"/>
        <property type="gene ID" value="NP_5184A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702286"/>
    <dbReference type="KEGG" id="nph:NP_5184A"/>
    <dbReference type="eggNOG" id="arCOG02911">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029417_0_0_2"/>
    <dbReference type="OrthoDB" id="148042at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055713">
        <property type="entry name" value="DUF7289"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055729">
        <property type="entry name" value="DUF7305"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23960">
        <property type="entry name" value="DUF7289"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23981">
        <property type="entry name" value="DUF7305"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7305" evidence="3">
        <location>
            <begin position="436"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="410"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50683.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="594" mass="62028" checksum="0FC2E8C92040321C" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZL5</accession>
    <name>A0A1U7EZL5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Homolog to virus protein HRPV1-VP8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5342A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324371.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZL5"/>
    <dbReference type="STRING" id="348780.NP_5342A"/>
    <dbReference type="EnsemblBacteria" id="CAI50762">
        <property type="protein sequence ID" value="CAI50762"/>
        <property type="gene ID" value="NP_5342A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702341"/>
    <dbReference type="KEGG" id="nph:NP_5342A"/>
    <dbReference type="eggNOG" id="arCOG08166">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043371_0_0_2"/>
    <dbReference type="OrthoDB" id="192786at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="359"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="364"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="378" mass="41955" checksum="B82AE09A74103EDC" modified="2017-05-10" version="1">MTDASWSAAELADQIRDGDVDAHIDAVLADDQDVGDVMLTMEHAGVDDGPLAEMLSRKGQTDMLRQARQSGDVASMNAATGLSESRTDATGYQRLIDACRPAAQQILIKGPKGSGKTAKALEIVRRLWQDDIIDKVLTNIGRDDPDDPAPEDHDAVEFAEDISRYLEFAKEPGEKVAIFDEFSTSGNAYTGQSDVEAVMSRTINAFRKSEGGSLRTIFIGHENDNDIHPLVKKQSDVVIQADGKVDEGLIDCITVYRGWNAYMTDEVWFRVRGLRDIPEWSDWNYSTNYFAHLEWDLDEPEKQINRGQLIDNWEAYQEPDEDPIGDGSSDEATKYVDCRGTKTDGSDCGATVTHESGYCHAHRSQWDGEPDPRLEGDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZL7</accession>
    <name>A0A1U7EZL7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Homolog to phage PhiH1 repressor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_5362A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324380.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZL7"/>
    <dbReference type="STRING" id="348780.NP_5362A"/>
    <dbReference type="EnsemblBacteria" id="CAI50772">
        <property type="protein sequence ID" value="CAI50772"/>
        <property type="gene ID" value="NP_5362A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702351"/>
    <dbReference type="KEGG" id="nph:NP_5362A"/>
    <dbReference type="eggNOG" id="arCOG03924">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161782_1_0_2"/>
    <dbReference type="OrthoDB" id="285635at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="99" mass="11567" checksum="EC3DE20192C625D2" modified="2017-05-10" version="1">MSDRQHRHPAEWMCMLDERILEHLEREGWSTASLIEQATTMNASESRTRERLRMLTDCGLVAPIFEGSRMYELTSEGQRYLRGELDAKKHIEQPNPHAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZP2</accession>
    <name>A0A1U7EZP2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alpha/beta hydrolase fold protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_5378A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324388.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZP2"/>
    <dbReference type="STRING" id="348780.NP_5378A"/>
    <dbReference type="ESTHER" id="natpd-q3im79">
        <property type="family name" value="6_AlphaBeta_hydrolase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI50780">
        <property type="protein sequence ID" value="CAI50780"/>
        <property type="gene ID" value="NP_5378A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702358"/>
    <dbReference type="KEGG" id="nph:NP_5378A"/>
    <dbReference type="eggNOG" id="arCOG01648">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066961_1_0_2"/>
    <dbReference type="OrthoDB" id="7531at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052382">
        <property type="entry name" value="ABHD10_acyl-thioesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16138">
        <property type="entry name" value="MYCOPHENOLIC ACID ACYL-GLUCURONIDE ESTERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16138:SF7">
        <property type="entry name" value="PALMITOYL-PROTEIN THIOESTERASE ABHD10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="2">
        <location>
            <begin position="51"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="238" mass="26331" checksum="92A727BA349AFA1F" modified="2017-05-10" version="1">MPVEHTIELEAGEELAAVYHPCGGDRWVFFCHGFRSDKHGSYEERCEQAVAADFNAVRFDFRGSGDSDRPFVETSLSTRIADLEAVVDHFDPPSYALFGSSFGAKTAFHAAADAPRLRALVGRAPVTYNRVFDAYREAVEREGRLQLDADHAISSAFFEDFETYDFEAAAAQVDVPVALFHGRADATVPLESCLDAVGALETDVRLQTYTDEGHRFSEAAEQRLREAAFAWLDDSYGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZQ2</accession>
    <name>A0A1U7EZQ2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3469B</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CCI69582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZQ2"/>
    <dbReference type="STRING" id="348780.NP_3469B"/>
    <dbReference type="EnsemblBacteria" id="CCI69582">
        <property type="protein sequence ID" value="CCI69582"/>
        <property type="gene ID" value="NP_3469B"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3469B"/>
    <dbReference type="eggNOG" id="arCOG08918">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_195712_3_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCI69582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="78" mass="8552" checksum="8E216A8437420B66" modified="2017-05-10" version="1">MGRLLLQQSLYTSTRLAQTTMATRLYQASVLALYQLSIVFGIAMLPLALVANRAGIQLPVDRLVSRLGSAYERSLPEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILU0</accession>
    <name>Q3ILU0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ABC transporter permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3304A</name>
        <name evidence="3" type="ordered locus">NP_7028A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>PL23</name>
    </geneLocation>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
            <strain evidence="3">Gabara</strain>
            <plasmid evidence="3">PL23</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CR936259">
        <property type="protein sequence ID" value="CAI50930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323364.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILU0"/>
    <dbReference type="STRING" id="348780.NP_3304A"/>
    <dbReference type="EnsemblBacteria" id="CAI49743">
        <property type="protein sequence ID" value="CAI49743"/>
        <property type="gene ID" value="NP_3304A"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI50930">
        <property type="protein sequence ID" value="CAI50930"/>
        <property type="gene ID" value="NP_7028A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703474"/>
    <dbReference type="KEGG" id="nph:NP_3304A"/>
    <dbReference type="KEGG" id="nph:NP_7028A"/>
    <dbReference type="HOGENOM" id="CLU_2646032_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="76" mass="7965" checksum="6605111D9496DEB4" modified="2005-11-08" version="1">MFGAFIRAVLSAGAAVLIAAILSFILGFFLPFLGPEDELLYRSFAAVAEHNLLVMMLAVCAALVARAVVEARPGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILU9</accession>
    <name>Q3ILU9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3286A</name>
        <name evidence="2" type="ordered locus">NP_7010A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>PL23</name>
    </geneLocation>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
            <strain evidence="2">Gabara</strain>
            <plasmid evidence="2">PL23</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CR936259">
        <property type="protein sequence ID" value="CAI50921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323355.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILU9"/>
    <dbReference type="STRING" id="348780.NP_3286A"/>
    <dbReference type="EnsemblBacteria" id="CAI49734">
        <property type="protein sequence ID" value="CAI49734"/>
        <property type="gene ID" value="NP_3286A"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI50921">
        <property type="protein sequence ID" value="CAI50921"/>
        <property type="gene ID" value="NP_7010A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703465"/>
    <dbReference type="KEGG" id="nph:NP_3286A"/>
    <dbReference type="KEGG" id="nph:NP_7010A"/>
    <dbReference type="HOGENOM" id="CLU_1567201_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="170" mass="19194" checksum="FB61B710DF0A5903" modified="2005-11-08" version="1">MSSDDDERQDRAPRRMIHDMRRRVQMVRNQYWDEGVEGQVSVQTHKELAAACIQYYDVLYEFRDESVLDDGDFPNIAPLRQRLGQRVERHQPSAGRGRGVSTQTVPAITEIPVEEILQLTEDLDDLAKKLGFGAQARETTPHDDIGHEDLAALLEARGQDDALEKVPGGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILX4</accession>
    <name>Q3ILX4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_6232A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324493.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILX4"/>
    <dbReference type="EnsemblBacteria" id="CAI50896">
        <property type="protein sequence ID" value="CAI50896"/>
        <property type="gene ID" value="NP_6232A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694647"/>
    <dbReference type="KEGG" id="nph:NP_6232A"/>
    <dbReference type="HOGENOM" id="CLU_1521824_0_0_2"/>
    <dbReference type="OrthoDB" id="204732at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="183" mass="18921" checksum="C3F7D81A02BF922D" modified="2005-11-08" version="1">MESPNSDSLQGAVEEYGPRTDHRDIAAGYAAAVGAISASVLYIASVWVIDSSLFTLEWNPYFTALEFYWVVYSSLAGLVVTIPAAFLVGVAGERLSPSKTKSSGMLKGAVGTVATYAVAFVLLSALLFVTGAGSTGTGTALFTAVELAGLIVGVGFALTWWLTIPIGCAVGCVYTTRQSATSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILY4</accession>
    <name>Q3ILY4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Deoxyribodipyrimidine photolyase</fullName>
            <ecNumber evidence="8">4.1.99.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">phr3</name>
        <name evidence="8" type="ordered locus">NP_6210A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8 9">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="4">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DNA photolyase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.3" evidence="8"/>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50885.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324483.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILY4"/>
    <dbReference type="EnsemblBacteria" id="CAI50885">
        <property type="protein sequence ID" value="CAI50885"/>
        <property type="gene ID" value="NP_6210A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694600"/>
    <dbReference type="KEGG" id="nph:NP_6210A"/>
    <dbReference type="HOGENOM" id="CLU_010348_7_0_2"/>
    <dbReference type="OrthoDB" id="11721at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003904">
        <property type="term" value="F:deoxyribodipyrimidine photo-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009416">
        <property type="term" value="P:response to light stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.579.10">
        <property type="entry name" value="DNA Cyclobutane Dipyrimidine Photolyase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036134">
        <property type="entry name" value="Crypto/Photolyase_FAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036155">
        <property type="entry name" value="Crypto/Photolyase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005101">
        <property type="entry name" value="Cryptochr/Photolyase_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002081">
        <property type="entry name" value="Cryptochrome/DNA_photolyase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018394">
        <property type="entry name" value="DNA_photolyase_1_CS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006050">
        <property type="entry name" value="DNA_photolyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11455">
        <property type="entry name" value="CRYPTOCHROME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11455:SF9">
        <property type="entry name" value="CRYPTOCHROME CIRCADIAN CLOCK 5 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00875">
        <property type="entry name" value="DNA_photolyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03441">
        <property type="entry name" value="FAD_binding_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00147">
        <property type="entry name" value="DNAPHOTLYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48173">
        <property type="entry name" value="Cryptochrome/photolyase FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52425">
        <property type="entry name" value="Cryptochrome/photolyase, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00394">
        <property type="entry name" value="DNA_PHOTOLYASES_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51645">
        <property type="entry name" value="PHR_CRY_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0157">Chromophore</keyword>
    <keyword evidence="2 4 5" id="KW-0274">FAD</keyword>
    <keyword evidence="1 4 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Photolyase/cryptochrome alpha/beta" evidence="7">
        <location>
            <begin position="45"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="116"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="578"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="138"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="585"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="599"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="309"/>
            <end position="313"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="343"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="444"/>
            <end position="446"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR602081-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI50885.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="616" mass="68214" checksum="04D1FA0FED28F8CD" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM73</accession>
    <name>Q3IM73_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_6012A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324394.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM73"/>
    <dbReference type="EnsemblBacteria" id="CAI50786">
        <property type="protein sequence ID" value="CAI50786"/>
        <property type="gene ID" value="NP_6012A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694580"/>
    <dbReference type="KEGG" id="nph:NP_6012A"/>
    <dbReference type="HOGENOM" id="CLU_123158_0_0_2"/>
    <dbReference type="OrthoDB" id="211802at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="189" mass="20530" checksum="75A2B3B6174B5F38" modified="2005-11-08" version="1">MSGRRLRTVVADTSALVSLAVPRADATVGTDAPDPFQYLLTSCDVFVPSEVVSELRDITQYQDIHGAAASNVLAARSYYTVTDPYEREDTPDSRPTFGLDDGETDGIVLANALDVDGFLTDEFGGTNFPLIHAVLQGPRIVPTPQLIVDYARNGHLSPEAARTLITTISPHRSWENSPYVARLVALLEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q3INE3</accession>
    <name>DNAG_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA primase DnaG</fullName>
            <ecNumber evidence="1">2.7.7.101</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dnaG</name>
        <name type="ordered locus">NP_4538A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ssDNA + n NTP = ssDNA/pppN(pN)n-1 hybrid + (n-1) diphosphate.</text>
            <dbReference type="EC" id="2.7.7.101"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds two Mg(2+) per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a ternary complex with MCM helicase and DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal DnaG primase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.101" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323975.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3INE3"/>
    <dbReference type="SMR" id="Q3INE3"/>
    <dbReference type="STRING" id="348780.NP_4538A"/>
    <dbReference type="EnsemblBacteria" id="CAI50360">
        <property type="protein sequence ID" value="CAI50360"/>
        <property type="gene ID" value="NP_4538A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702092"/>
    <dbReference type="KEGG" id="nph:NP_4538A"/>
    <dbReference type="eggNOG" id="arCOG04281">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034626_0_0_2"/>
    <dbReference type="OrthoDB" id="8643at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000178">
        <property type="term" value="C:exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01029">
        <property type="entry name" value="TOPRIM_primases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00007">
        <property type="entry name" value="DNA_primase_DnaG_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050219">
        <property type="entry name" value="DnaG_primase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020607">
        <property type="entry name" value="Primase_DnaG_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034154">
        <property type="entry name" value="TOPRIM_DnaG/twinkle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003108">
        <property type="entry name" value="PRK04031.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30313">
        <property type="entry name" value="DNA PRIMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30313:SF2">
        <property type="entry name" value="DNA PRIMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13662">
        <property type="entry name" value="Toprim_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56731">
        <property type="entry name" value="DNA primase core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-0639">Primosome</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000240464" description="DNA primase DnaG">
        <location>
            <begin position="1"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="Toprim" evidence="1">
        <location>
            <begin position="167"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="267"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="313"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="383"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="509" mass="52822" checksum="98400B7A47B4A959" modified="2005-11-08" version="1">MQDTAKYLVHADITADGVVERSDVVGAVFGQTEGLLGDELDLRELQDASKVGRIDVEIDSENGQSFGRITIATSLDRVETAILGGALETIDRVGPCRSAIEVRKIEDVRSAKRREVVERAKSLLDGAFDESMRSSRDLVEEVRESVRVEDITDYEGLPAGPAVADSDAIVVVEGRADVLTLLQYGIKNAVAVEGTNVPEAVASLTETRTVTAFLDNDRGGELIRKELGQVGDIDYVATPPDGKCVEDLARHEVMSALREKVPYGRFKQAASDDADPEAAEQRTGEAAGATAADSEPAATDGIGGVTTDAEGKPVSSPESPAESPAADETAAVSAGTTPADAEEAAVDGATKTSTAGEEPNTPDDELTATSEAAEPGSAETKGESTAAEAPESSADGPAAAGASTDEQPKTLRGHVSDVIEAETGTFRLLDAEFAPLAAGDAGDVFEAVADAETVPAAVVVDGEASQRLLDIAAQRGIDHVVAASTGEFVKRPTSVRVRTATQLLNPEKA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-28" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IPG8</accession>
    <name>FEN_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Flap endonuclease 1</fullName>
            <shortName evidence="2">FEN-1</shortName>
            <ecNumber evidence="2">3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Flap structure-specific endonuclease 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">fen</name>
        <name type="ordered locus">NP_3784A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with PCNA. PCNA stimulates the nuclease activity without altering cleavage specificity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323600.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IPG8"/>
    <dbReference type="SMR" id="Q3IPG8"/>
    <dbReference type="STRING" id="348780.NP_3784A"/>
    <dbReference type="EnsemblBacteria" id="CAI49983">
        <property type="protein sequence ID" value="CAI49983"/>
        <property type="gene ID" value="NP_3784A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702700"/>
    <dbReference type="KEGG" id="nph:NP_3784A"/>
    <dbReference type="eggNOG" id="arCOG04050">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032444_0_0_2"/>
    <dbReference type="OrthoDB" id="9593at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008409">
        <property type="term" value="F:5'-3' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09903">
        <property type="entry name" value="H3TH_FEN1-Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09867">
        <property type="entry name" value="PIN_FEN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00614">
        <property type="entry name" value="Fen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023426">
        <property type="entry name" value="Flap_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019973">
        <property type="entry name" value="Flap_endonuc_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019974">
        <property type="entry name" value="XPG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03674">
        <property type="entry name" value="fen_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081:SF9">
        <property type="entry name" value="FLAP ENDONUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="FLAP ENDONUCLEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00841">
        <property type="entry name" value="XPG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0269">Exonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000200238" description="Flap endonuclease 1">
        <location>
            <begin position="1"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-domain">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="I-domain">
        <location>
            <begin position="120"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with PCNA" evidence="2">
        <location>
            <begin position="320"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00614"/>
        </source>
    </evidence>
    <sequence length="328" mass="36413" checksum="CD0BC1844C1AB36B" modified="2005-11-08" version="1">MGNADLRTLAAIEPKPFDELGGSVVAVDAHNWLYRYLTTTVKFTRSEAYTTADGEEVANLIGVVQGLPKFFEHDVTPVFVFDGGVSDLKADEVEKRRDQRERYEAQLEAAKERDETDAAEIAALESRTQRLTDTIVETTRELLARLDVPVVEAPAEGEAQAAHMARRGDADYVGSEDYDALLLGAPYTLRGLTSNGDPECMDFEATLSTHDLSWEGLVDAAILMGTDFNEGLSGVGPKTAVKLIHEHGDLRSVLAARDDDIPHADRIRELFLDPAVTDDYDYDTDIDPDFERAREFVTETWGVPADEVERGFERIEESVIQTGLDRWT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IPM0</accession>
    <name>RSMA_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable ribosomal RNA small subunit methyltransferase A</fullName>
            <ecNumber evidence="1">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">16S rRNA dimethyladenosine transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">16S rRNA dimethylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">S-adenosylmethionine-6-N',N'-adenosyl(rRNA) dimethyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rsmA</name>
        <name evidence="1" type="synonym">ksgA</name>
        <name type="ordered locus">NP_3678A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323548.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IPM0"/>
    <dbReference type="SMR" id="Q3IPM0"/>
    <dbReference type="STRING" id="348780.NP_3678A"/>
    <dbReference type="EnsemblBacteria" id="CAI49930">
        <property type="protein sequence ID" value="CAI49930"/>
        <property type="gene ID" value="NP_3678A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702875"/>
    <dbReference type="KEGG" id="nph:NP_3678A"/>
    <dbReference type="eggNOG" id="arCOG04131">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041220_0_2_2"/>
    <dbReference type="OrthoDB" id="9883at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000179">
        <property type="term" value="F:rRNA (adenine-N6,N6-)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.100">
        <property type="entry name" value="Ribosomal RNA adenine dimethylase-like, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00607">
        <property type="entry name" value="16SrRNA_methyltr_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023165">
        <property type="entry name" value="rRNA_Ade_diMease-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020596">
        <property type="entry name" value="rRNA_Ade_Mease_Trfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020598">
        <property type="entry name" value="rRNA_Ade_methylase_Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011530">
        <property type="entry name" value="rRNA_adenine_dimethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00755">
        <property type="entry name" value="ksgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011486">
        <property type="entry name" value="PRK14896.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727:SF7">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00398">
        <property type="entry name" value="RrnaAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00650">
        <property type="entry name" value="rADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01131">
        <property type="entry name" value="RRNA_A_DIMETH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51689">
        <property type="entry name" value="SAM_RNA_A_N6_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000257383" description="Probable ribosomal RNA small subunit methyltransferase A">
        <location>
            <begin position="1"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00607"/>
        </source>
    </evidence>
    <sequence length="274" mass="29863" checksum="900BF82076125C9A" modified="2005-11-08" version="1">MRDPDALLVRAGVRGDPERDQHFLIDDRVLDRLPGYLPDSADTDHVLEIGGGTGALTDRLLDVADRVTVIERDPGLATFLTDEFADEIEAGSLTVIEGDALEVPLPDFTASVSNLPYGVSSEIAFRLLPEKRPLVLMFQQEFAERMAAEPGTDEYGRLSVTAGHYADVEVVEPVPKEAFSPPPAVESAVVRTTPREPPYEVPDDELFMRLVRAVFTQRRKTMRNAVRNTTHISGIEDAEAVVEAAGEDLMSKRAGNVPPEAFARLAAIAAEVDG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ISU0</accession>
    <name>UPP_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Uracil phosphoribosyltransferase</fullName>
            <ecNumber evidence="1">2.4.2.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">UMP pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">UPRTase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">upp</name>
        <name type="ordered locus">NP_1408A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of uracil and 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to UMP and diphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>UMP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + uracil</text>
            <dbReference type="Rhea" id="RHEA:13017"/>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit. The magnesium is bound as Mg-PRPP.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Allosterically activated by GTP.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uracil: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPRTase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.9" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322430.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ISU0"/>
    <dbReference type="SMR" id="Q3ISU0"/>
    <dbReference type="STRING" id="348780.NP_1408A"/>
    <dbReference type="EnsemblBacteria" id="CAI48795">
        <property type="protein sequence ID" value="CAI48795"/>
        <property type="gene ID" value="NP_1408A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703445"/>
    <dbReference type="KEGG" id="nph:NP_1408A"/>
    <dbReference type="eggNOG" id="arCOG04128">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067096_2_0_2"/>
    <dbReference type="OrthoDB" id="80352at2157"/>
    <dbReference type="UniPathway" id="UPA00574">
        <property type="reaction ID" value="UER00636"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004845">
        <property type="term" value="F:uracil phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044206">
        <property type="term" value="P:UMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006223">
        <property type="term" value="P:uracil salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01218_A">
        <property type="entry name" value="Upp_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034331">
        <property type="entry name" value="Upp_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005765">
        <property type="entry name" value="UPRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050054">
        <property type="entry name" value="UPRTase/APRTase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001097">
        <property type="entry name" value="PRK00129.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01091">
        <property type="entry name" value="upp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32315">
        <property type="entry name" value="ADENINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32315:SF4">
        <property type="entry name" value="URACIL PHOSPHORIBOSYLTRANSFERASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14681">
        <property type="entry name" value="UPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0021">Allosteric enzyme</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000053747" description="Uracil phosphoribosyltransferase">
        <location>
            <begin position="1"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="145"/>
            <end position="153"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>uracil</name>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="214"/>
            <end position="216"/>
        </location>
        <ligand>
            <name>uracil</name>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01218"/>
        </source>
    </evidence>
    <sequence length="223" mass="23671" checksum="29035BC600807C7C" modified="2005-11-08" version="1">MPIEDRDDAHVITHALAKHTLSELRSDETDQVAFRNGLVELGRLCGYEIIDGMMDTEYVSITTPLAETTGEVVKGLDDVVIVNVLRAATPFVEGLVEAFPHARQGVISAGRDEAAGMNEDGEFPITVDYVKLPDIDADDTVIVADPILATGSTMVAVLEEVLEQGTPERLVVLSAVSAPPGLARVNDSIPSADVLTVSVDERLDEDGYIVPGVGDAGDRAFGT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-07-10" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IT46</accession>
    <name>MFNA_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable L-aspartate decarboxylase</fullName>
            <shortName evidence="1">ADC</shortName>
            <ecNumber evidence="1">4.1.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mfnA</name>
        <name type="synonym">gadD</name>
        <name type="ordered locus">NP_1194A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the decarboxylation of L-aspartate to produce beta-alanine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-aspartate + H(+) = beta-alanine + CO2</text>
            <dbReference type="Rhea" id="RHEA:19497"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:57966"/>
            <dbReference type="EC" id="4.1.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the group II decarboxylase family. MfnA subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.11" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322324.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IT46"/>
    <dbReference type="SMR" id="Q3IT46"/>
    <dbReference type="STRING" id="348780.NP_1194A"/>
    <dbReference type="EnsemblBacteria" id="CAI48688">
        <property type="protein sequence ID" value="CAI48688"/>
        <property type="gene ID" value="NP_1194A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702733"/>
    <dbReference type="KEGG" id="nph:NP_1194A"/>
    <dbReference type="eggNOG" id="arCOG00027">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028929_2_1_2"/>
    <dbReference type="OrthoDB" id="56891at2157"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004068">
        <property type="term" value="F:aspartate 1-decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01610">
        <property type="entry name" value="MfnA_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050477">
        <property type="entry name" value="GrpII_AminoAcid_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020931">
        <property type="entry name" value="MfnA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03812">
        <property type="entry name" value="tyr_de_CO2_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42735">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42735:SF6">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE LYASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0210">Decarboxylase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000293190" description="Probable L-aspartate decarboxylase">
        <location>
            <begin position="1"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01610"/>
        </source>
    </evidence>
    <sequence length="350" mass="37765" checksum="76537F9F1D9F5CF9" modified="2005-11-08" version="1">MQRAEPQEFSRVLSSMCTEPHPAAREAAERFLATNPGDPGTYETVSKLEREAVDMLGEVAGLPDAAGYIASGGTEANIQAVRIARNRADTRTPNFVAPASAHFSFRKAADILGVELRTAPLEDYRANLDGVAELIDSDTALVVGVAGTTEYGRVDPIPALADMAADAGALCHVDAAWGGFVLPFTEHAWDFDDADIHTMTIDPHKMGQAAVPAGGLLARGPELLDELAIDTPYLESTSQVTLTGTRSGAGVASAAAVMDELWRDGYRQQYETAQTNAHWLAAEVESRGFDVVDPVLPIVAMDLPYDLVADLRERGWRLSRTEADEARIVCMPHVTRSMLEEFLTDLDRLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ITG5</accession>
    <name>Q3ITG5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">dUTP diphosphatase</fullName>
            <ecNumber evidence="3">3.6.1.23</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">dut</name>
        <name evidence="3" type="ordered locus">NP_0954A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.1.23" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322204.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ITG5"/>
    <dbReference type="STRING" id="348780.NP_0954A"/>
    <dbReference type="EnsemblBacteria" id="CAI48568">
        <property type="protein sequence ID" value="CAI48568"/>
        <property type="gene ID" value="NP_0954A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702660"/>
    <dbReference type="KEGG" id="nph:NP_0954A"/>
    <dbReference type="eggNOG" id="arCOG04048">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103451_1_0_2"/>
    <dbReference type="OrthoDB" id="45265at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008829">
        <property type="term" value="F:dCTP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004170">
        <property type="term" value="F:dUTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006229">
        <property type="term" value="P:dUTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011962">
        <property type="entry name" value="dCTP_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002598">
        <property type="entry name" value="PRK02253.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42680">
        <property type="entry name" value="DCTP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42680:SF1">
        <property type="entry name" value="DEOXYURIDINE 5'-TRIPHOSPHATE NUCLEOTIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22769">
        <property type="entry name" value="DCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="dUTPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="153" mass="16586" checksum="6D111FB12F7CC9A5" modified="2005-11-08" version="1">MFRSGSFVADRIDPTTAAQQQPNGVDLTLSAVFEQASPGRIDQDGKSIGDRQELSADDGVFRLQPGGYIVQYGETISIPEEHVGFVYPRSSLLRNSCMLNTAVWDAGYEGKGEGLLEVHHEIEIEAGARIAQLVLAGADHEGTYDGSYQGERI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-12-06" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ITJ2</accession>
    <name>RFCS_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Replication factor C small subunit</fullName>
            <shortName evidence="1">RFC small subunit</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Clamp loader small subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rfcS</name>
        <name type="synonym">rfcA</name>
        <name type="ordered locus">NP_0900A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heteromultimer composed of small subunits (RfcS) and large subunits (RfcL).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the activator 1 small subunits family. RfcS subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322177.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ITJ2"/>
    <dbReference type="SMR" id="Q3ITJ2"/>
    <dbReference type="STRING" id="348780.NP_0900A"/>
    <dbReference type="EnsemblBacteria" id="CAI48541">
        <property type="protein sequence ID" value="CAI48541"/>
        <property type="gene ID" value="NP_0900A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703100"/>
    <dbReference type="KEGG" id="nph:NP_0900A"/>
    <dbReference type="eggNOG" id="arCOG00469">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042324_2_1_2"/>
    <dbReference type="OrthoDB" id="7928at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.272.10:FF:000029">
        <property type="entry name" value="Replication factor C small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000952">
        <property type="entry name" value="Replication factor C subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01509">
        <property type="entry name" value="RfcS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023748">
        <property type="entry name" value="Rep_factor-C_ssu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013748">
        <property type="entry name" value="Rep_factorC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001679">
        <property type="entry name" value="PRK00440.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF20">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08542">
        <property type="entry name" value="Rep_fac_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000135979" description="Replication factor C small subunit">
        <location>
            <begin position="1"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="52"/>
            <end position="59"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01509"/>
        </source>
    </evidence>
    <sequence length="325" mass="35722" checksum="C81EAC71206F1851" modified="2005-11-08" version="1">MSEGDADGGGREIWIEKYRPQTLDDIVGHESITERLKQYIAQNDLPHLLFAGPAGVGKTTAATAIAKEVYGDDWRENFLELNASDQRGIDVVRDRIKSFARASFGGYDHRIIFLDEADALTSDAQSALRRTMEQFSDNTRFILSCNYSSQIIDPIQSRCAVFRFSPLGDAAVDEQIRIIADTEGIELTDDGVDALVYAADGDMRKAINGLQAAAVMGGTVDEEAVYTITSTARPEEIREMVTEAMDGDFTAARSQLETLLTDVGIAGGDIIDQLHRSVWEFDLEEREAVQLMERIGEADYRITAGASEQLQLEALLAALARGNET</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-05-20" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IUB3</accession>
    <name>NAC_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nascent polypeptide-associated complex protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nac</name>
        <name type="ordered locus">NP_0354A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Contacts the emerging nascent chain on the ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Interacts with the ribosome. Binds ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAC-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321906.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IUB3"/>
    <dbReference type="SMR" id="Q3IUB3"/>
    <dbReference type="STRING" id="348780.NP_0354A"/>
    <dbReference type="EnsemblBacteria" id="CAI48268">
        <property type="protein sequence ID" value="CAI48268"/>
        <property type="gene ID" value="NP_0354A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700828"/>
    <dbReference type="KEGG" id="nph:NP_0354A"/>
    <dbReference type="eggNOG" id="arCOG04061">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_146475_1_0_2"/>
    <dbReference type="OrthoDB" id="53273at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.30">
        <property type="entry name" value="Nascent polypeptide-associated complex domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00814">
        <property type="entry name" value="NAC_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038187">
        <property type="entry name" value="NAC_A/B_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005231">
        <property type="entry name" value="NAC_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002715">
        <property type="entry name" value="Nas_poly-pep-assoc_cplx_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00264">
        <property type="entry name" value="archaeal-type nascent polypeptide-associated complex protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01849">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01407">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51151">
        <property type="entry name" value="NAC_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0653">Protein transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_1000083770" description="Nascent polypeptide-associated complex protein">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="NAC-A/B" evidence="1">
        <location>
            <begin position="7"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="53"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="124" mass="13448" checksum="C4D86CCE5B0B029F" modified="2005-11-08" version="1">MFGGGGGLNPRKMKQMMNQMGIDLEEIDAEEVVIRTADEELVFDDAEVQLMDAQGQQTYQVVGEPESRERGDSGSEDDSETESGGEFSEDDVEIVAQRAGVSESTARETLEETGDLAAAVQKLE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IUH3</accession>
    <name>MUTS_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA mismatch repair protein MutS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mutS</name>
        <name type="ordered locus">NP_0232A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein is involved in the repair of mismatches in DNA. It is possible that it carries out the mismatch recognition step. This protein has a weak ATPase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321846.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IUH3"/>
    <dbReference type="SMR" id="Q3IUH3"/>
    <dbReference type="STRING" id="348780.NP_0232A"/>
    <dbReference type="EnsemblBacteria" id="CAI48207">
        <property type="protein sequence ID" value="CAI48207"/>
        <property type="gene ID" value="NP_0232A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702952"/>
    <dbReference type="KEGG" id="nph:NP_0232A"/>
    <dbReference type="eggNOG" id="arCOG02897">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002472_3_1_2"/>
    <dbReference type="OrthoDB" id="146065at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1420.10:FF:000007">
        <property type="entry name" value="DNA mismatch repair protein MutS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000870">
        <property type="entry name" value="MutS protein homolog 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1420.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.10">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.110">
        <property type="entry name" value="MutS, connector domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00096">
        <property type="entry name" value="MutS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005748">
        <property type="entry name" value="DNA_mismatch_repair_MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007695">
        <property type="entry name" value="DNA_mismatch_repair_MutS-lik_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017261">
        <property type="entry name" value="DNA_mismatch_repair_MutS/MSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007861">
        <property type="entry name" value="DNA_mismatch_repair_MutS_clamp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016151">
        <property type="entry name" value="DNA_mismatch_repair_MutS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007860">
        <property type="entry name" value="DNA_mmatch_repair_MutS_con_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01070">
        <property type="entry name" value="mutS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003810">
        <property type="entry name" value="PRK05399.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF34">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01624">
        <property type="entry name" value="MutS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05188">
        <property type="entry name" value="MutS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05190">
        <property type="entry name" value="MutS_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037677">
        <property type="entry name" value="DNA_mis_repair_Msh6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55271">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53150">
        <property type="entry name" value="DNA repair protein MutS, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000224425" description="DNA mismatch repair protein MutS">
        <location>
            <begin position="1"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="623"/>
            <end position="630"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00096"/>
        </source>
    </evidence>
    <sequence length="856" mass="93618" checksum="137382EE53FF635E" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-05-20" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IUP2</accession>
    <name>RL40_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein eL40</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L40e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl40e</name>
        <name type="ordered locus">NP_0094A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL40 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321777.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IUP2"/>
    <dbReference type="SMR" id="Q3IUP2"/>
    <dbReference type="STRING" id="348780.NP_0094A"/>
    <dbReference type="EnsemblBacteria" id="CAI48138">
        <property type="protein sequence ID" value="CAI48138"/>
        <property type="gene ID" value="NP_0094A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700761"/>
    <dbReference type="KEGG" id="nph:NP_0094A"/>
    <dbReference type="eggNOG" id="arCOG04049">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_205640_0_0_2"/>
    <dbReference type="OrthoDB" id="45138at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00788">
        <property type="entry name" value="Ribosomal_eL40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023657">
        <property type="entry name" value="Ribosomal_eL40_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001975">
        <property type="entry name" value="Ribosomal_eL40_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038587">
        <property type="entry name" value="Ribosomal_eL40_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003161">
        <property type="entry name" value="PRK04136.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39649">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L40E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39649:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01020">
        <property type="entry name" value="Ribosomal_L40e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01377">
        <property type="entry name" value="Ribosomal_L40e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_1000083599" description="Large ribosomal subunit protein eL40">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="49" mass="5761" checksum="5BFC141E2DA4567E" modified="2005-11-08" version="1">MASFEEAEERILDKMICMRCNARNPKRADSCRKCGYKKLRPKAKERRAA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-17" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IUS6</accession>
    <name>FLUC2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fluoride-specific ion channel FluC 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fluC2</name>
        <name evidence="1" type="synonym">crcB2</name>
        <name type="ordered locus">NP_0026A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Fluoride-specific ion channel. Important for reducing fluoride concentration in the cell, thus reducing its toxicity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>fluoride(in) = fluoride(out)</text>
            <dbReference type="Rhea" id="RHEA:76159"/>
            <dbReference type="ChEBI" id="CHEBI:17051"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:76160"/>
        </physiologicalReaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Na(+) is not transported, but it plays an essential structural role and its presence is essential for fluoride channel function.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the fluoride channel Fluc/FEX (TC 1.A.43) family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321743.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IUS6"/>
    <dbReference type="SMR" id="Q3IUS6"/>
    <dbReference type="STRING" id="348780.NP_0026A"/>
    <dbReference type="EnsemblBacteria" id="CAI48104">
        <property type="protein sequence ID" value="CAI48104"/>
        <property type="gene ID" value="NP_0026A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702630"/>
    <dbReference type="KEGG" id="nph:NP_0026A"/>
    <dbReference type="eggNOG" id="arCOG04701">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114342_2_1_2"/>
    <dbReference type="OrthoDB" id="304656at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062054">
        <property type="term" value="F:fluoride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140114">
        <property type="term" value="P:cellular detoxification of fluoride"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00454">
        <property type="entry name" value="FluC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003691">
        <property type="entry name" value="FluC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02537">
        <property type="entry name" value="CRCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0407">Ion channel</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0915">Sodium</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000252968" description="Fluoride-specific ion channel FluC 2">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <note>structural</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00454"/>
        </source>
    </evidence>
    <sequence length="116" mass="11043" checksum="DB73268D808A884E" modified="2005-11-08" version="1">MALLTALAVGAGGAAGAVARYAVGLSVGRRAVDTGLVNVFGSLLFGVAIGADFGGAPAVAVTVGFCGAFTTFSSFAVETVRLAEDGQRLAAAGNAVGTLAAALLAVFLGIALGAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ET90</accession>
    <name>A0A1U7ET90_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aminopeptidase (Homolog to leucyl aminopeptidase / aminopeptidase T)</fullName>
            <ecNumber evidence="2">3.4.11.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pepB1</name>
        <name evidence="2" type="ordered locus">NP_0014A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.4.11.-" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321737.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ET90"/>
    <dbReference type="STRING" id="348780.NP_0014A"/>
    <dbReference type="EnsemblBacteria" id="CAI48098">
        <property type="protein sequence ID" value="CAI48098"/>
        <property type="gene ID" value="NP_0014A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703011"/>
    <dbReference type="KEGG" id="nph:NP_0014A"/>
    <dbReference type="eggNOG" id="arCOG01890">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062630_0_0_2"/>
    <dbReference type="OrthoDB" id="371826at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052170">
        <property type="entry name" value="M29_Exopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058739">
        <property type="entry name" value="NicX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34448">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34448:SF1">
        <property type="entry name" value="BLL6088 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26233">
        <property type="entry name" value="NicX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144052">
        <property type="entry name" value="Thermophilic metalloprotease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="325" mass="33548" checksum="1A2B512E0CABF3A5" modified="2017-05-10" version="1">MADELEIESPSAVASAAETAVEQCLAIEAGERCVVVTDDKRAPIGEALYEAALAVTDDATIVRYPPGPQHGAEPPAPVAAAMADADAFLAPTTKSLSHTRARSEASEAGVRGATLPGITESVFTTGLDADYDAIAETTATVYAAVADAERVRVTSPQGTDITFEIGDREWLEDTGIVHEPGSFSNLPAGETFVSPTSADGTYVVDGTIRPHGRLDADQTVTFEVEDGFVTDVDDAAIRDELETAAAEVGEDAYNLAELGIGTNLGVEALVGSVLLDEKAAGTVHIAVGDDAGIGGETDAPIHMDGILREPTVLVDGEELSLPSAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETA6</accession>
    <name>A0A1U7ETA6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0058A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321759.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETA6"/>
    <dbReference type="STRING" id="348780.NP_0058A"/>
    <dbReference type="EnsemblBacteria" id="CAI48120">
        <property type="protein sequence ID" value="CAI48120"/>
        <property type="gene ID" value="NP_0058A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700748"/>
    <dbReference type="KEGG" id="nph:NP_0058A"/>
    <dbReference type="eggNOG" id="arCOG10814">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_172993_0_0_2"/>
    <dbReference type="OrthoDB" id="205887at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058328">
        <property type="entry name" value="DUF8015"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26047">
        <property type="entry name" value="DUF8015"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="80" mass="8482" checksum="08383EB145C01330" modified="2017-05-10" version="1">MAGYYEQLLGAILASLLMGALASLHAAVTLHEGLVGGSLVATLFLYEALFRNPPVSPRKPAVASAAFFWHLGLLLLVFTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETB6</accession>
    <name>A0A1U7ETB6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF5786 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0088A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321774.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETB6"/>
    <dbReference type="STRING" id="348780.NP_0088A"/>
    <dbReference type="EnsemblBacteria" id="CAI48135">
        <property type="protein sequence ID" value="CAI48135"/>
        <property type="gene ID" value="NP_0088A"/>
    </dbReference>
    <dbReference type="GeneID" id="41345074"/>
    <dbReference type="KEGG" id="nph:NP_0088A"/>
    <dbReference type="eggNOG" id="arCOG03012">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_209486_0_0_2"/>
    <dbReference type="OrthoDB" id="166327at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043902">
        <property type="entry name" value="DUF5786"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19099">
        <property type="entry name" value="DUF5786"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5786" evidence="2">
        <location>
            <begin position="3"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="58" mass="6566" checksum="BD45C031B5FD9B40" modified="2017-05-10" version="1">MGFGSYDESEQENQQLDTDDIEDGGQSTLPTHDGEVEFEYDDASSEDLLETYERLQGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETJ5</accession>
    <name>A0A1U7ETJ5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphoserine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">O-phosphoserine phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">serB</name>
        <name evidence="13" type="ordered locus">NP_0274A</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HAD-like hydrolase superfamily. SerB family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.3" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETJ5"/>
    <dbReference type="STRING" id="348780.NP_0274A"/>
    <dbReference type="EnsemblBacteria" id="CAI48228">
        <property type="protein sequence ID" value="CAI48228"/>
        <property type="gene ID" value="NP_0274A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_0274A"/>
    <dbReference type="eggNOG" id="arCOG01158">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036368_4_3_2"/>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00198"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036424">
        <property type="term" value="F:L-phosphoserine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050582">
        <property type="entry name" value="HAD-like_SerB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004469">
        <property type="entry name" value="PSP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01488">
        <property type="entry name" value="HAD-SF-IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00338">
        <property type="entry name" value="serB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43344">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43344:SF2">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12710">
        <property type="entry name" value="HAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0718">Serine biosynthesis</keyword>
    <feature type="domain" description="Rhodanese" evidence="12">
        <location>
            <begin position="100"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="30"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR604469-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAI48228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="234" mass="24385" checksum="F7EB32D1B5E55EFA" modified="2017-05-10" version="1">MPGVRSSTSNRNRTICDPSLPRAGMLIAFDFDGTLSDSEMTVLLGEEAGVADEIDDITERAMNDELSYAESLYARAELLDGLESSAVDAAFNRVALRPGSADVIEALSEAGHHVAILTGGFEQGVERALETEGVSVDTVVANRLPARNGTLTGDAEGPLIEGTKDDALADLADEVGVSMDDTVAIGDGANDLPMLEVAGLAVGYEPKPAVEPHCDTVVASMAELLDLLEAKAVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETQ7</accession>
    <name>A0A1U7ETQ7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tryptophan--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Tryptophanyl-tRNA synthetase</fullName>
            <shortName evidence="8">TrpRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 12" type="primary">trpS</name>
        <name evidence="12" type="ordered locus">NP_0442A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the attachment of tryptophan to tRNA(Trp).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>tRNA(Trp) + L-tryptophan + ATP = L-tryptophyl-tRNA(Trp) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24080"/>
            <dbReference type="Rhea" id="RHEA-COMP:9671"/>
            <dbReference type="Rhea" id="RHEA-COMP:9705"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78535"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 9">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.2" evidence="8"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321948.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETQ7"/>
    <dbReference type="STRING" id="348780.NP_0442A"/>
    <dbReference type="EnsemblBacteria" id="CAI48312">
        <property type="protein sequence ID" value="CAI48312"/>
        <property type="gene ID" value="NP_0442A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703419"/>
    <dbReference type="KEGG" id="nph:NP_0442A"/>
    <dbReference type="eggNOG" id="arCOG01887">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032621_3_1_2"/>
    <dbReference type="OrthoDB" id="371821at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004830">
        <property type="term" value="F:tryptophan-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006436">
        <property type="term" value="P:tryptophanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000207">
        <property type="entry name" value="Tryptophan--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00140_A">
        <property type="entry name" value="Trp_tRNA_synth_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002306">
        <property type="entry name" value="Trp-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020653">
        <property type="entry name" value="Tryptophan-tRNA-ligase_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008926">
        <property type="entry name" value="PRK12285.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008928">
        <property type="entry name" value="PRK12285.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10055:SF5">
        <property type="entry name" value="TRYPTOPHAN--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10055">
        <property type="entry name" value="TRYPTOPHANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01039">
        <property type="entry name" value="TRNASYNTHTRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="479"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="8">
        <location>
            <begin position="97"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="8">
        <location>
            <begin position="396"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI48312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="516" mass="56999" checksum="12BD985578F76716" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETT9</accession>
    <name>A0A1U7ETT9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">FAD-dependent oxidoreductase (Homolog to geranylgeranyl reductase)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0512A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321983.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETT9"/>
    <dbReference type="STRING" id="348780.NP_0512A"/>
    <dbReference type="EnsemblBacteria" id="CAI48347">
        <property type="protein sequence ID" value="CAI48347"/>
        <property type="gene ID" value="NP_0512A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700879"/>
    <dbReference type="KEGG" id="nph:NP_0512A"/>
    <dbReference type="eggNOG" id="arCOG00570">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024648_0_1_2"/>
    <dbReference type="OrthoDB" id="46008at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011777">
        <property type="entry name" value="Geranylgeranyl_Rdtase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050407">
        <property type="entry name" value="Geranylgeranyl_reductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02032">
        <property type="entry name" value="GG-red-SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685:SF21">
        <property type="entry name" value="DEHYDROGENASE (FLAVOPROTEIN)-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685">
        <property type="entry name" value="GERANYLGERANYL DIPHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="1">
        <location>
            <begin position="98"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="361" mass="38959" checksum="F8896BFFCCC02C83" modified="2017-05-10" version="1">MYDFIVVGAGPAGSRFARAAAERDHGVLVLESGAIGEPLACSGHVSLDVWAFVPETARERLHQNDIYGARFHLGGADSRAYPFYKETVVSNAIDRVGLDRTLAECARDAGAEVREQHTVTAVTEGSDRATVTANGPDGTEQFAAKVVCGADGPRSKVRSQVGLPEPDELLHGVLGFEPTPDSQDFVDVHLTVPGFFAWRIPRGEAGAEYGLAVSPGDDAPARFEALVEDYGVDIERRCSGAIPIGPPDRTVSERTFLLGDAAGQTKPFTGGGILYGMRAADVAARVLDPDRRSTLYRYEHAWRDELARDQRLGRLIRAGYSLPKPVQRAGMAAFAGEIGVHMDRPSTLFSVDQLRAMAPWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETU3</accession>
    <name>A0A1U7ETU3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Small CPxCG-related zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0506A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321980.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETU3"/>
    <dbReference type="STRING" id="348780.NP_0506A"/>
    <dbReference type="DNASU" id="3700876"/>
    <dbReference type="EnsemblBacteria" id="CAI48344">
        <property type="protein sequence ID" value="CAI48344"/>
        <property type="gene ID" value="NP_0506A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700876"/>
    <dbReference type="KEGG" id="nph:NP_0506A"/>
    <dbReference type="eggNOG" id="arCOG01917">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1870800_0_0_2"/>
    <dbReference type="OrthoDB" id="241505at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026870">
        <property type="entry name" value="Zinc_ribbon_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13240">
        <property type="entry name" value="Zn_Ribbon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="118"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc-ribbon" evidence="2">
        <location>
            <begin position="4"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="136" mass="14347" checksum="90F3AFF93F7DDB63" modified="2017-05-10" version="1">MPECRNCGATLREEYRFCPMCATPQTDEASRRLNQYIEQRATKRQASGGTASSNGAENDGTLRGRLQYAAGYVAIVLGLATVTAVAGVFFLLAGLFLLPPIHGRIESAVGRRLGPAPTAGVAGVLAAFGLVVFVFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETY3</accession>
    <name>A0A1U7ETY3_NATPD</name>
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        <submittedName>
            <fullName evidence="2">Origin-associated protein OapB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">oapB</name>
        <name evidence="2" type="ordered locus">NP_0592A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322023.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETY3"/>
    <dbReference type="STRING" id="348780.NP_0592A"/>
    <dbReference type="EnsemblBacteria" id="CAI48387">
        <property type="protein sequence ID" value="CAI48387"/>
        <property type="gene ID" value="NP_0592A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700899"/>
    <dbReference type="KEGG" id="nph:NP_0592A"/>
    <dbReference type="eggNOG" id="arCOG04416">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145183_0_0_2"/>
    <dbReference type="OrthoDB" id="74375at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012017">
        <property type="entry name" value="OapB-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09846">
        <property type="entry name" value="OapB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004977">
        <property type="entry name" value="UCP004977"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="133" mass="14511" checksum="CADE8D52E13551CD" modified="2017-05-10" version="1">MPEITADDGDGPDDDGIQIDMISAERMEGMRTMEKIRLILDGVHDGNIVILEQGLEPDEESKLIEVTMSEINPDGFTGIEIETYPGEESGGSGLLGRIMGKDDSNKLTVIGPANRIETLHKDETLISTLITRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETZ6</accession>
    <name>A0A1U7ETZ6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0630A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322042.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETZ6"/>
    <dbReference type="STRING" id="348780.NP_0630A"/>
    <dbReference type="EnsemblBacteria" id="CAI48406">
        <property type="protein sequence ID" value="CAI48406"/>
        <property type="gene ID" value="NP_0630A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700911"/>
    <dbReference type="KEGG" id="nph:NP_0630A"/>
    <dbReference type="eggNOG" id="arCOG04666">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120318_0_0_2"/>
    <dbReference type="OrthoDB" id="284647at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055523">
        <property type="entry name" value="DUF7097"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23382">
        <property type="entry name" value="DUF7097"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="174" mass="19547" checksum="BB3C2E84B8750ADD" modified="2017-05-10" version="1">MEETPQGTPVGVEDPYAVVERCDFLTDDGRCRAAVDGDAAAAAFVGGERDDGRCPAADPEGGHSWSDCPSFRARQRSRTCRRCGLAERRLAHDDERPLLEEHHLRYPDDKRREHSHEITVYLCRWCHSKVHAGSRIDDDADPDPEALAEAARRRKREQTELGFESAADRYGEDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU21</accession>
    <name>A0A1U7EU21_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0684A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322069.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU21"/>
    <dbReference type="STRING" id="348780.NP_0684A"/>
    <dbReference type="EnsemblBacteria" id="CAI48433">
        <property type="protein sequence ID" value="CAI48433"/>
        <property type="gene ID" value="NP_0684A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700929"/>
    <dbReference type="KEGG" id="nph:NP_0684A"/>
    <dbReference type="eggNOG" id="arCOG06340">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_191139_0_0_2"/>
    <dbReference type="OrthoDB" id="263502at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055895">
        <property type="entry name" value="DUF7472"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24284">
        <property type="entry name" value="DUF7472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="65" mass="6693" checksum="690D4A5BB32D81A4" modified="2017-05-10" version="1">MDRDAVAEAIVAFVGAGTLAGLIIWVGTAYTDGVLTEDGGLALVGAIAFFIVFMSLLGVGLSRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU52</accession>
    <name>A0A1U7EU52_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">F420H2:NADP oxidoreductase</fullName>
            <ecNumber evidence="3">1.5.1.40</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">npdG</name>
        <name evidence="3" type="ordered locus">NP_0756A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.5.1.40" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322105.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU52"/>
    <dbReference type="STRING" id="348780.NP_0756A"/>
    <dbReference type="EnsemblBacteria" id="CAI48469">
        <property type="protein sequence ID" value="CAI48469"/>
        <property type="gene ID" value="NP_0756A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700954"/>
    <dbReference type="KEGG" id="nph:NP_0756A"/>
    <dbReference type="eggNOG" id="arCOG00457">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076368_1_1_2"/>
    <dbReference type="OrthoDB" id="8635at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102261">
        <property type="term" value="F:8-hydroxy-5-deazaflavin:NADPH oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070967">
        <property type="term" value="F:coenzyme F420 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008823">
        <property type="term" value="F:cupric reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052851">
        <property type="term" value="F:ferric-chelate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006740">
        <property type="term" value="P:NADPH regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010185">
        <property type="entry name" value="NpdG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051267">
        <property type="entry name" value="STEAP_metalloreductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01915">
        <property type="entry name" value="npdG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14239">
        <property type="entry name" value="DUDULIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14239:SF0">
        <property type="entry name" value="F420-DEPENDENT NADP REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase catalytic N-terminal" evidence="2">
        <location>
            <begin position="2"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="222" mass="23413" checksum="AFA894D93BCE4714" modified="2017-05-10" version="1">MRIALLGGTGDIGRGLALRWGRDTDHELLVGSRDPEKAREAAEAYIEKLDSYGADAEIKGFENAMAADRADVVVLAVPAYHLVDTVEAVADRLDDDTVLVTPAVGMQRDEDGFHYNRPGAGSVAALAADAKPDGVPLVGAFHNLPAGGLSDLDADFEWDTLVFGDDDDAKAIVTELAAGIDGLRPLDVGGLANAPEVEAVTPLLINVAMENDDLHDLGVRFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU59</accession>
    <name>A0A1U7EU59_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Helix-hairpin-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0804A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322129.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU59"/>
    <dbReference type="STRING" id="348780.NP_0804A"/>
    <dbReference type="EnsemblBacteria" id="CAI48493">
        <property type="protein sequence ID" value="CAI48493"/>
        <property type="gene ID" value="NP_0804A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700967"/>
    <dbReference type="KEGG" id="nph:NP_0804A"/>
    <dbReference type="eggNOG" id="arCOG06234">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103621_0_0_2"/>
    <dbReference type="OrthoDB" id="202878at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010995">
        <property type="entry name" value="DNA_repair_Rad51/TF_NusA_a-hlx"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14520">
        <property type="entry name" value="HHH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47794">
        <property type="entry name" value="Rad51 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="11"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="57"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="175" mass="17736" checksum="F856CE2F41B892B8" modified="2017-05-10" version="1">MGVFKLLRTVFGLGRSEGTSGTEVSVEHEPEGGDDTDELSEADEDTDGASDADDAVAAETDAAASTGSMAEEPTSDPETAAEPAEAGAGVSDHSGTDTVAAESAEAAGPVPDEPEGGDEPVENVNGIGPAYAKRLSDAGIETVTQLLDADPEAVADETDLSAKRISGWQENAEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUB7</accession>
    <name>A0A1U7EUB7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="7">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">uvrD</name>
        <name evidence="11" type="ordered locus">NP_0930A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 13">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322192.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUB7"/>
    <dbReference type="STRING" id="348780.NP_0930A"/>
    <dbReference type="EnsemblBacteria" id="CAI48556">
        <property type="protein sequence ID" value="CAI48556"/>
        <property type="gene ID" value="NP_0930A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701002"/>
    <dbReference type="KEGG" id="nph:NP_0930A"/>
    <dbReference type="eggNOG" id="arCOG00797">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030778_0_0_2"/>
    <dbReference type="OrthoDB" id="88793at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000725">
        <property type="term" value="P:recombinational repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070:SF2">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE SRS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00580">
        <property type="entry name" value="UvrD-helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9 11" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 9 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="10">
        <location>
            <begin position="1"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="12"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI48556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="613" mass="69111" checksum="918606E8E6DF82E3" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUC0</accession>
    <name>A0A1U7EUC0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0934A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322194.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUC0"/>
    <dbReference type="STRING" id="348780.NP_0934A"/>
    <dbReference type="EnsemblBacteria" id="CAI48558">
        <property type="protein sequence ID" value="CAI48558"/>
        <property type="gene ID" value="NP_0934A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701004"/>
    <dbReference type="KEGG" id="nph:NP_0934A"/>
    <dbReference type="eggNOG" id="arCOG08183">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2597841_0_0_2"/>
    <dbReference type="OrthoDB" id="238439at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055955">
        <property type="entry name" value="DUF7533"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24377">
        <property type="entry name" value="DUF7533"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="79" mass="7692" checksum="B1DF699194C5E4A7" modified="2017-05-10" version="1">MGLVDTLTLFGVVALAAPIGLLGIEFLVGGRPVAGAGFLAVAVGVAVGVYARPSLKSALAARAVDAVEDGDGNESGDKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUC6</accession>
    <name>A0A1U7EUC6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0952A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322203.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUC6"/>
    <dbReference type="STRING" id="348780.NP_0952A"/>
    <dbReference type="EnsemblBacteria" id="CAI48567">
        <property type="protein sequence ID" value="CAI48567"/>
        <property type="gene ID" value="NP_0952A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701009"/>
    <dbReference type="KEGG" id="nph:NP_0952A"/>
    <dbReference type="eggNOG" id="arCOG03029">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2243747_0_0_2"/>
    <dbReference type="OrthoDB" id="225920at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043952">
        <property type="entry name" value="DUF5783"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19095">
        <property type="entry name" value="DUF5783"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="103" mass="12075" checksum="D85FD07912B7F497" modified="2017-05-10" version="1">MDAETFDEQKYVDLFPQLQQAYKNVFNRLNEEYDSTLIHAIDQQVLNESEPFYDDDAEGFYVELPDDPYDRLTGVAVEEETFESVLETYIGELEAELERTFGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUJ3</accession>
    <name>A0A1U7EUJ3_NATPD</name>
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        <submittedName>
            <fullName evidence="4">Cobalt-precorrin-5A hydrolase</fullName>
            <ecNumber evidence="4">3.7.1.12</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cbiG</name>
        <name evidence="4" type="ordered locus">NP_1118A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.7.1.12" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322286.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUJ3"/>
    <dbReference type="STRING" id="348780.NP_1118A"/>
    <dbReference type="EnsemblBacteria" id="CAI48650">
        <property type="protein sequence ID" value="CAI48650"/>
        <property type="gene ID" value="NP_1118A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702571"/>
    <dbReference type="KEGG" id="nph:NP_1118A"/>
    <dbReference type="eggNOG" id="arCOG00651">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028397_0_2_2"/>
    <dbReference type="OrthoDB" id="4722at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043779">
        <property type="term" value="F:cobalt-precorrin-5A acetaldehyde-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.180">
        <property type="entry name" value="CobE/GbiG C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052553">
        <property type="entry name" value="CbiG_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021744">
        <property type="entry name" value="CbiG_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002750">
        <property type="entry name" value="CobE/GbiG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036518">
        <property type="entry name" value="CobE/GbiG_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038029">
        <property type="entry name" value="GbiG_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004465">
        <property type="entry name" value="PRK05788.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37477">
        <property type="entry name" value="COBALT-PRECORRIN-5A HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37477:SF1">
        <property type="entry name" value="COBALT-PRECORRIN-5A HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01890">
        <property type="entry name" value="CbiG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11760">
        <property type="entry name" value="CbiG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159672">
        <property type="entry name" value="CbiG N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159664">
        <property type="entry name" value="CobE/GbiG C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cobalamin synthesis G N-terminal" evidence="3">
        <location>
            <begin position="60"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="CobE/GbiG C-terminal" evidence="2">
        <location>
            <begin position="203"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF11760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="321" mass="34110" checksum="79D52B6056309FEB" modified="2017-05-10" version="1">MSDTNDSDGGSCSTPDSDGEVAEEIGIVSFERKRPVAEEIRAELSDDYERIDILDYHGEVFADNWGDYDCFVGLMASGIAMRKTAGLLDDKWEDPAVVVIDEELTWAIPLTGGHHGANQVAHDLSKLGAVPAMTTASEAAGKQGVESKAKALDSHVVNGDSTVATNLAVLNDELGPVERLDGPRAVLVGDDVTVLKRNSDDGVVLGTGTVDGVRKEQVLDAWEAALGETEHDISDVEFVATATRKEGEAGLYEAAEEIGVGVVCFDKETLLDHEGPSPSRAKELIGWPGIAEASAIAGGRDHELLREKERFDEAVTVAIGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUJ8</accession>
    <name>A0A1U7EUJ8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cobalt-precorrin-6B C15-methyltransferase (Decarboxylating)</fullName>
            <ecNumber evidence="6">2.1.1.196</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">cbiT</name>
        <name evidence="6" type="synonym">cobL2</name>
        <name evidence="6" type="ordered locus">NP_1124A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.196" evidence="6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322289.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUJ8"/>
    <dbReference type="STRING" id="348780.NP_1124A"/>
    <dbReference type="EnsemblBacteria" id="CAI48653">
        <property type="protein sequence ID" value="CAI48653"/>
        <property type="gene ID" value="NP_1124A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702576"/>
    <dbReference type="KEGG" id="nph:NP_1124A"/>
    <dbReference type="eggNOG" id="arCOG00977">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094143_1_0_2"/>
    <dbReference type="OrthoDB" id="6027at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014008">
        <property type="entry name" value="Cbl_synth_MTase_CbiT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050714">
        <property type="entry name" value="Cobalamin_biosynth_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02469">
        <property type="entry name" value="CbiT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43182">
        <property type="entry name" value="COBALT-PRECORRIN-6B C(15)-METHYLTRANSFERASE (DECARBOXYLATING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43182:SF1">
        <property type="entry name" value="COBALT-PRECORRIN-7 C(5)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01135">
        <property type="entry name" value="PCMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="3 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI48653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="179" mass="18986" checksum="307E4470C8E4D06D" modified="2017-05-10" version="1">MTRTALPHDAKAGPTKPEVRSVTLGKLNLTPEDHFAEVGACTGAVTIEAARQAGTVTAVERKETRIETAAKNLDANGIEDVDLRQAEAPNGLPTDADALFLGGSRNYGAVLDFAAENGIDRIVMNVSRLEVAGDAVRAFRERDLFEEVIQLQVSTGYELAGATSFESDNPVYVIVGGAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUL2</accession>
    <name>A0A1U7EUL2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1136A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322295.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUL2"/>
    <dbReference type="STRING" id="348780.NP_1136A"/>
    <dbReference type="EnsemblBacteria" id="CAI48659">
        <property type="protein sequence ID" value="CAI48659"/>
        <property type="gene ID" value="NP_1136A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701062"/>
    <dbReference type="KEGG" id="nph:NP_1136A"/>
    <dbReference type="eggNOG" id="arCOG00498">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048478_2_1_2"/>
    <dbReference type="OrthoDB" id="6433at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050662">
        <property type="entry name" value="Sec-metab_biosynth-thioest"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23131">
        <property type="entry name" value="ENDORIBONUCLEASE LACTB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23131:SF0">
        <property type="entry name" value="ENDORIBONUCLEASE LACTB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="17"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="257" mass="27913" checksum="C2A4E75032B80D8F" modified="2017-05-10" version="1">MHIERLPVAVPTRAPSGETAAYCIGREEALLVDPPAPDTRIEQVLDRVEHIAVTHHHPDHVGAVSEYARIADATVWCRYGRGDGFEAATGIRPDRTFRDGTEVPAGRSNVIVRETPGHAPEHVAFDAAGALVVGDLAVAAGSVVVGAPEGDMRAYLTSLRRVWAMDPERLYPAHGPVIDEPRKVCERLINHRRDRERRVLAAVEDGSRTVASILDTAYEKDLDGVRDLAGQTVRAHLDKLAHEGHITWDGAHADPAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUS8</accession>
    <name>A0A1U7EUS8_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Preprotein translocase subunit SecG</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 12">Protein transport protein Sec61 subunit beta homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12 14" type="primary">secG</name>
        <name evidence="14" type="ordered locus">NP_1308A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Involved in protein export. The function of the beta subunit is unknown, but it may be involved in stabilization of the trimeric complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the protein translocase complex. Heterotrimer consisting of alpha (SecY), beta (SecG) and gamma (SecE) subunits. Can form oligomers of the heterotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the SEC61-beta family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322380.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUS8"/>
    <dbReference type="STRING" id="348780.NP_1308A"/>
    <dbReference type="EnsemblBacteria" id="CAI48745">
        <property type="protein sequence ID" value="CAI48745"/>
        <property type="gene ID" value="NP_1308A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703203"/>
    <dbReference type="KEGG" id="nph:NP_1308A"/>
    <dbReference type="eggNOG" id="arCOG02957">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_208205_0_0_2"/>
    <dbReference type="OrthoDB" id="43651at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00751">
        <property type="entry name" value="SecG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023531">
        <property type="entry name" value="Preprot_translocase_SecG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016482">
        <property type="entry name" value="SecG/Sec61-beta/Sbh"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002318">
        <property type="entry name" value="PRK01253.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03911">
        <property type="entry name" value="Sec61_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0811">Translocation</keyword>
    <keyword evidence="6 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 12" id="KW-0813">Transport</keyword>
    <feature type="topological domain" description="Cytoplasmic" evidence="12">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAI48745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="53" mass="5578" checksum="1B752274AA5A1861" modified="2017-05-10" version="1">MSSGQNSGGLMSSAGLVRYFDEESRNAPTMDPRTVVAFGVLLGVFVLMLNALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUT5</accession>
    <name>A0A1U7EUT5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1338A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322395.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUT5"/>
    <dbReference type="STRING" id="348780.NP_1338A"/>
    <dbReference type="EnsemblBacteria" id="CAI48760">
        <property type="protein sequence ID" value="CAI48760"/>
        <property type="gene ID" value="NP_1338A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701118"/>
    <dbReference type="KEGG" id="nph:NP_1338A"/>
    <dbReference type="eggNOG" id="arCOG04524">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_157002_0_0_2"/>
    <dbReference type="OrthoDB" id="330759at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055898">
        <property type="entry name" value="DUF7475"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24287">
        <property type="entry name" value="DUF7475"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="124" mass="13042" checksum="BACDA46544E24F48" modified="2017-05-10" version="1">MTRNASGEAGQLTPLHWVGIGAALVTAGVHFFLGAQDLDGLFGISFLFAGIGFLAGIAAIVVGWRRRLVYLLGVPFTAGQIVLWLALNQPIPPISAPEAVDKVAQVVLVAVLVVLLYREGLPGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUU0</accession>
    <name>A0A1U7EUU0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uroporphyrinogen-III synthase</fullName>
            <ecNumber evidence="2">4.2.1.75</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">hemD</name>
        <name evidence="2" type="ordered locus">NP_1330A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.2.1.75" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322391.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUU0"/>
    <dbReference type="STRING" id="348780.NP_1330A"/>
    <dbReference type="EnsemblBacteria" id="CAI48756">
        <property type="protein sequence ID" value="CAI48756"/>
        <property type="gene ID" value="NP_1330A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702808"/>
    <dbReference type="KEGG" id="nph:NP_1330A"/>
    <dbReference type="eggNOG" id="arCOG02048">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011276_9_5_2"/>
    <dbReference type="OrthoDB" id="15395at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004852">
        <property type="term" value="F:uroporphyrinogen-III synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006780">
        <property type="term" value="P:uroporphyrinogen III biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06578">
        <property type="entry name" value="HemD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10090">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036108">
        <property type="entry name" value="4pyrrol_syn_uPrphyn_synt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003754">
        <property type="entry name" value="4pyrrol_synth_uPrphyn_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039793">
        <property type="entry name" value="UROS/Hem4"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004587">
        <property type="entry name" value="PRK05928.2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40082">
        <property type="entry name" value="BLR5956 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40082:SF1">
        <property type="entry name" value="BLR5956 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02602">
        <property type="entry name" value="HEM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69618">
        <property type="entry name" value="HemD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrapyrrole biosynthesis uroporphyrinogen III synthase" evidence="1">
        <location>
            <begin position="19"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="239" mass="24490" checksum="ABD2E39EFF7CB2F8" modified="2017-05-10" version="1">MTSRVAFFRPDDERAVEAAVTVSELGAEPLSDPMLEVEPTGRTPRDDAAYTILTSKTGAELVADTGWQPGGELVAIGTATAGALEAAGFDVDRLPEEFSSSGIVSELEADAPGTRIEVARSDHGSDVLTDGLVDAGGYVHETVLYRLVRPDGAGDSAVAAADGDLAGACFTSPLTVEYFLEAAADRGCRDAALEGLNTAVVGAIGDPTREAAAEHGIEVDVVPGTAEFAALAEAVVDQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUY7</accession>
    <name>A0A1U7EUY7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glutaredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">grx2</name>
        <name evidence="2" type="ordered locus">NP_1416A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322434.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUY7"/>
    <dbReference type="STRING" id="348780.NP_1416A"/>
    <dbReference type="EnsemblBacteria" id="CAI48799">
        <property type="protein sequence ID" value="CAI48799"/>
        <property type="gene ID" value="NP_1416A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702763"/>
    <dbReference type="KEGG" id="nph:NP_1416A"/>
    <dbReference type="eggNOG" id="arCOG02609">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026126_8_2_2"/>
    <dbReference type="OrthoDB" id="73564at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011767">
        <property type="entry name" value="GLR_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45288">
        <property type="entry name" value="THIOREDOXIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45288:SF1">
        <property type="entry name" value="THIOREDOXIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00195">
        <property type="entry name" value="GLUTAREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="1">
        <location>
            <begin position="4"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="89" mass="9806" checksum="1A48DF1E4C31D289" modified="2017-05-10" version="1">MSEPDITLYRLQACPFCERVVAVLDELGLAYRSRFVEARHSRRDVVKRLTGARTVPALVDDRTGVTMSESANIVEYLRATYGDGGGGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV12</accession>
    <name>A0A1U7EV12_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">SufB domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">sufB1</name>
        <name evidence="5" type="synonym">abc31p1</name>
        <name evidence="5" type="ordered locus">NP_1502A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the iron-sulfur cluster assembly SufBD family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322476.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV12"/>
    <dbReference type="STRING" id="348780.NP_1502A"/>
    <dbReference type="EnsemblBacteria" id="CAI48842">
        <property type="protein sequence ID" value="CAI48842"/>
        <property type="gene ID" value="NP_1502A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702457"/>
    <dbReference type="KEGG" id="nph:NP_1502A"/>
    <dbReference type="eggNOG" id="arCOG01715">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026231_0_1_2"/>
    <dbReference type="OrthoDB" id="372168at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055346">
        <property type="entry name" value="Fe-S_cluster_assembly_SufBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010231">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000825">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037284">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045595">
        <property type="entry name" value="SufBD_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01980">
        <property type="entry name" value="sufB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30508">
        <property type="entry name" value="FES CLUSTER ASSEMBLY PROTEIN SUF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30508:SF1">
        <property type="entry name" value="UPF0051 PROTEIN ABCI8, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01458">
        <property type="entry name" value="SUFBD_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19295">
        <property type="entry name" value="SufBD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101960">
        <property type="entry name" value="Stabilizer of iron transporter SufD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUF system FeS cluster assembly SufBD N-terminal" evidence="4">
        <location>
            <begin position="139"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="SUF system FeS cluster assembly SufBD core" evidence="3">
        <location>
            <begin position="213"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00043967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF19295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI48842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="476" mass="53342" checksum="9A21D2D1234FA96C" modified="2017-05-10" version="1">MSSEEDHIKETDTEARFEFKKEESSAFKTEKGLTEETIRVISEDKDEPEWMLERRLRALEQFQQMPMPTDWPGQPDLSDVDIDEIVPYIRPDIEARGGAEEWEDLPEEIQDTFDKLGIPEAEKNALSGVGAQYESEIVYQNMQEQWEEKGVIFMDMDKAVQEHPELVREYFMTKCVPPSDNKFAALHGAIWSGGSFVYVPEGVTVEMPIQAYFRMNSENMGQFEHTLIIAEENSEVHYIEGCSAPKYSAFNLHSGGVEVFVKEGAHVQYSTVQNWSKNTYNLNTKRAIVEKDGTMEWVSGSMGSKATMLYPCTILKGPGATDNHITIAFAGEGQDIDTGAKVYHNAPNTKSTIESKSISKDGGRTNYRGLVHIADGAEGSSTAVECDALMFDNESTSDTMPYMEIEESKVDVAHEATVGKIGDEDVFYLQSRGLDDDDAKQMIVSGFIEPITEELPIEYAVELNRLIELEMEGSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV20</accession>
    <name>A0A1U7EV20_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TIGR04031 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1544A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322497.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV20"/>
    <dbReference type="STRING" id="348780.NP_1544A"/>
    <dbReference type="EnsemblBacteria" id="CAI48863">
        <property type="protein sequence ID" value="CAI48863"/>
        <property type="gene ID" value="NP_1544A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701176"/>
    <dbReference type="KEGG" id="nph:NP_1544A"/>
    <dbReference type="eggNOG" id="arCOG06266">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_163110_0_0_2"/>
    <dbReference type="OrthoDB" id="168567at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.520">
        <property type="entry name" value="Phenylacetic acid degradation B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023976">
        <property type="entry name" value="CHP04031_Htur1727"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009359">
        <property type="entry name" value="PaaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038693">
        <property type="entry name" value="PaaB_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04031">
        <property type="entry name" value="Htur_1727_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06243">
        <property type="entry name" value="PaaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="102" mass="11404" checksum="71DA089347EB7347" modified="2017-05-10" version="1">MVERTERRRIDDGDRAPNGREWELFVRSDSEDPLRHVGSVRAPDADGAYELACRLFAWYAEDVWVCPSADVTRYTTTELTEDAPTVTPDAADGEPATGSESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV88</accession>
    <name>A0A1U7EV88_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">NP_1640A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322545.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV88"/>
    <dbReference type="EnsemblBacteria" id="CAI48911">
        <property type="protein sequence ID" value="CAI48911"/>
        <property type="gene ID" value="NP_1640A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701209"/>
    <dbReference type="KEGG" id="nph:NP_1640A"/>
    <dbReference type="eggNOG" id="arCOG02364">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_58_2"/>
    <dbReference type="OrthoDB" id="327291at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004673">
        <property type="term" value="F:protein histidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050980">
        <property type="entry name" value="2C_sensor_his_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44936">
        <property type="entry name" value="SENSOR PROTEIN CREC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44936:SF10">
        <property type="entry name" value="SENSOR PROTEIN RSTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13188">
        <property type="entry name" value="PAS_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="143"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI48911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="354" mass="39837" checksum="7C9D7931D5EAE8ED" modified="2017-05-10" version="1">MDSAESPTPRDGDGNRWQHLIEHIQDAVVEFELVDDEPIVRDVNRAFVDVFGYERSTVLDAPLNNYIVPEWLDEEASSLDEETASGNVNYRRVRRQTADGLREFLYRGIPLVNATDRIDGFAVYTDLTDIVQQERRLQVLNRILRHNLRNRTTAIAGNTARLLEQLDEQSETSTRTAATIEREVEALRHLADEAGRIEQLTGSEDGEQSEVDVVPFLRGCVAEYTERYPASDFETDLPPTAMVKASHGLRAAVESLLENAVEHNPDPKPRVNVAVNNGAVDGWTDIRIEDDGPRIPERERRLVAGDADITPLHHGQGIGLWLAKWTTERFGGELAFETSEWGGNSVRLRFRSAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVB5</accession>
    <name>A0A1U7EVB5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7993 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1730A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322589.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVB5"/>
    <dbReference type="STRING" id="348780.NP_1730A"/>
    <dbReference type="EnsemblBacteria" id="CAI48956">
        <property type="protein sequence ID" value="CAI48956"/>
        <property type="gene ID" value="NP_1730A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701241"/>
    <dbReference type="KEGG" id="nph:NP_1730A"/>
    <dbReference type="eggNOG" id="arCOG04658">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159741_0_0_2"/>
    <dbReference type="OrthoDB" id="242585at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058306">
        <property type="entry name" value="DUF7993"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25956">
        <property type="entry name" value="DUF7993"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7993" evidence="1">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="126" mass="13293" checksum="03C7BC76BE187CB7" modified="2017-05-10" version="1">MVHNEIGRGERIAELLSSEVHGHDRGALGALSVTDAAEEVEPTADGALAFRIAAADEVLADVYVQPDRARVEFVAATEAAAATADKEALRVRPKAVEPPRTLVFVENGAEVKGALRVVRRAAADVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVD4</accession>
    <name>A0A1U7EVD4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF162 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1724A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322586.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVD4"/>
    <dbReference type="STRING" id="348780.NP_1724A"/>
    <dbReference type="EnsemblBacteria" id="CAI48953">
        <property type="protein sequence ID" value="CAI48953"/>
        <property type="gene ID" value="NP_1724A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701238"/>
    <dbReference type="KEGG" id="nph:NP_1724A"/>
    <dbReference type="eggNOG" id="arCOG04519">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090664_1_3_2"/>
    <dbReference type="OrthoDB" id="199019at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10420">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024185">
        <property type="entry name" value="FTHF_cligase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003741">
        <property type="entry name" value="LUD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43682">
        <property type="entry name" value="LACTATE UTILIZATION PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43682:SF1">
        <property type="entry name" value="LACTATE UTILIZATION PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02589">
        <property type="entry name" value="LUD_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LUD" evidence="1">
        <location>
            <begin position="66"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="169" mass="17446" checksum="030D26E4D9BC6A8B" modified="2017-05-10" version="1">MPASTIETFERAVGRTADGCHRTDTETFEETLSEVVETPAVGTPLSFEGVSFGEVAIEMDPSPAALTAAETGVTPAGLGIADYGTVTIDGRAAGDELVSLYPPRHVVVVDESDIVPDMAAAFERFEQTIGGDGPDSCVLATGPSATADMGTLVEGVHGPTEVHVVVVER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVE3</accession>
    <name>A0A1U7EVE3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ArsA family ATPase</fullName>
            <ecNumber evidence="3">3.6.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">arsA3</name>
        <name evidence="3" type="synonym">tpa08</name>
        <name evidence="3" type="ordered locus">NP_1818A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arsA ATPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.-.-" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322632.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVE3"/>
    <dbReference type="STRING" id="348780.NP_1818A"/>
    <dbReference type="EnsemblBacteria" id="CAI49000">
        <property type="protein sequence ID" value="CAI49000"/>
        <property type="gene ID" value="NP_1818A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703355"/>
    <dbReference type="KEGG" id="nph:NP_1818A"/>
    <dbReference type="eggNOG" id="arCOG02849">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040761_4_0_2"/>
    <dbReference type="OrthoDB" id="46198at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02035">
        <property type="entry name" value="ArsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025723">
        <property type="entry name" value="ArsA/GET3_ATPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016300">
        <property type="entry name" value="ATPase_ArsA/GET3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00345">
        <property type="entry name" value="GET3_arsA_TRC40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803">
        <property type="entry name" value="ARSENICAL PUMP-DRIVING ATPASE ARSENITE-TRANSLOCATING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803:SF3">
        <property type="entry name" value="ATPASE GET3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02374">
        <property type="entry name" value="ArsA_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ArsA/GET3 Anion-transporting ATPase-like" evidence="2">
        <location>
            <begin position="5"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="318" mass="35450" checksum="1E922432E83540AC" modified="2017-05-10" version="1">MVSFRFFGGKGGVGKTTVAAAHGVACARDGLETLVVSTDPAHSTSDVFDQQFGDVPQPVSAHDRLHAMEIDPQAEVDDHLQELRQQLGQQLSPAMVNEVSMQLEMAHQTPGAYEAALFDCFVDVMQNADDYDRVVFDTSPTGGTLRLLALPEYLEQWVERLRHKREKSIKNFERAAIGDQQARRMLEGDPIIDRLQERKRNFEFAGEVLRNEAAFTFVLNPDELSIHETRRALESLQEADLTVDGLVVNKLTPDPDPDETGTGAAFLRNRVSRERERLETIRSELEPPVVAEIQSRAEEITGDTLTAVAAELDTRPVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVF0</accession>
    <name>A0A1U7EVF0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1812A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
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            <dbReference type="DOI" id="10.1101/gr.3952905"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <location>
            <begin position="15"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="139" mass="15448" checksum="0FEE8E1B40BC0BB4" modified="2017-05-10" version="1">MQEAARNAFGVLPTWAQVLTLLVTALVGAKLIEVVGYYALHQADRLAKGEYDSLIIEETHAALYISIFLGGIYLSAQFVPQVNYPALRRSPRRDSLVEEGALSSLVKPVSPCKARRDLHCRQVSMSEQRRHLVECPALD</sequence>
</entry>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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        </source>
    </reference>
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    <dbReference type="EnsemblBacteria" id="CAI49007">
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    </dbReference>
    <dbReference type="GeneID" id="3703337"/>
    <dbReference type="KEGG" id="nph:NP_1832A"/>
    <dbReference type="eggNOG" id="arCOG02009">
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018461">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43478:SF1">
        <property type="entry name" value="NA+_H+ ANTIPORTER NHAC-LIKE C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03553">
        <property type="entry name" value="Na_H_antiporter"/>
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    </dbReference>
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        <location>
            <begin position="12"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="301"/>
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    <feature type="transmembrane region" description="Helical" evidence="7">
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            <end position="340"/>
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    </feature>
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            <end position="371"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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        <location>
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            <end position="568"/>
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            <dbReference type="ARBA" id="ARBA00004651"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF03553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI49007.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="568" mass="59680" checksum="4FF35E27CD608130" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVL9</accession>
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        <lineage>
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            <taxon>Stenosarchaea group</taxon>
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    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
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            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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    </dbReference>
    <dbReference type="GeneID" id="3701322"/>
    <dbReference type="KEGG" id="nph:NP_1962A"/>
    <dbReference type="eggNOG" id="arCOG03059">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085083_1_0_2"/>
    <dbReference type="OrthoDB" id="136483at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
    <dbReference type="NCBIfam" id="NF008528">
        <property type="entry name" value="PRK11463.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UPF0716 PROTEIN FXSA"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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            <end position="25"/>
        </location>
    </feature>
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    </feature>
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        <location>
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            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
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            <end position="176"/>
        </location>
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        <location>
            <begin position="179"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI49072.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="204" mass="21136" checksum="863DE21207AC64FA" modified="2017-05-10" version="1">MRLRVIGLLLLVPVVDILLLVVLATRLGPVTIGPVATVAVVVLTALLGLLFARAEGRHTLRKIQRKLANGDAPTNELLDGLLLIIAGTLMLTPGVITDFIGLLLVLPPTRYPIRIATKKYVVTPFLDDKTGGFASGNVYVGGFPGGDETAGQGFPGGGGPGGGFPGDASGPDAAGDTVEMGEADYEFTDPEPDEDDDTDSDRTA</sequence>
</entry>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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        </source>
    </reference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR007386">
        <property type="entry name" value="DUF447_N"/>
    </dbReference>
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        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
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        <property type="entry name" value="DUF447_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04289">
        <property type="entry name" value="DUF447_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF447" evidence="1">
        <location>
            <begin position="13"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="DUF447" evidence="2">
        <location>
            <begin position="130"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="189" mass="20969" checksum="D99EE79CE54A85C6" modified="2017-05-10" version="1">MPADWPVDLSGVTESVVTTRGPNGRWNVAALGLFAGEPVTARTWGRTRTWRNFSERGGGYVQFTHDPVDFVDAACSVREEADPVLDSADAWATVDVERLDDGEEGDTQWVEWALTPVEARVEREVVPTTNRGYYAVIEATVAASRLDVPGYDTETLRSRIDYFESVVDRCGGDREAAAFERLTEYADID</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVW0</accession>
    <name>A0A1U7EVW0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2212A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322824.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVW0"/>
    <dbReference type="STRING" id="348780.NP_2212A"/>
    <dbReference type="EnsemblBacteria" id="CAI49197">
        <property type="protein sequence ID" value="CAI49197"/>
        <property type="gene ID" value="NP_2212A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701378"/>
    <dbReference type="KEGG" id="nph:NP_2212A"/>
    <dbReference type="eggNOG" id="arCOG03942">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035890_0_0_2"/>
    <dbReference type="OrthoDB" id="203750at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="19"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="551" mass="60670" checksum="D9DDE46565DBF643" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVX6</accession>
    <name>A0A1U7EVX6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Glycosyltransferase AglD</fullName>
            <ecNumber evidence="11">2.4.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">aglD</name>
        <name evidence="11" type="ordered locus">NP_2244A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.-.-" evidence="11"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322840.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVX6"/>
    <dbReference type="STRING" id="348780.NP_2244A"/>
    <dbReference type="EnsemblBacteria" id="CAI49213">
        <property type="protein sequence ID" value="CAI49213"/>
        <property type="gene ID" value="NP_2244A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701390"/>
    <dbReference type="KEGG" id="nph:NP_2244A"/>
    <dbReference type="eggNOG" id="arCOG00897">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_474611_0_0_2"/>
    <dbReference type="OrthoDB" id="351177at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04188">
        <property type="entry name" value="DPG_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035518">
        <property type="entry name" value="DPG_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022791">
        <property type="entry name" value="L-PG_synthase/AglD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00374">
        <property type="entry name" value="flippase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859:SF105">
        <property type="entry name" value="DOLICHYL-PHOSPHATE BETA-D-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859">
        <property type="entry name" value="GLYCOSYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03706">
        <property type="entry name" value="LPG_synthase_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="248"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="279"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="365"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="407"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="474"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="505"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="537"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="10">
        <location>
            <begin position="7"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI49213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="602" mass="64963" checksum="62C75A64F2FAD413" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW29</accession>
    <name>A0A1U7EW29_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase</fullName>
            <ecNumber evidence="2 10">4.3.1.32</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">FO synthase subunit 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10 12" type="primary">cofG</name>
        <name evidence="12" type="ordered locus">NP_2398A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the radical-mediated synthesis of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO) from 5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil + S-adenosyl-L-methionine = 7,8-didemethyl-8-hydroxy-5-deazariboflavin + 5'-deoxyadenosine + L-methionine + NH4(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55204"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:59904"/>
            <dbReference type="ChEBI" id="CHEBI:85936"/>
            <dbReference type="EC" id="4.3.1.32"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="10">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Cofactor biosynthesis; coenzyme F0 biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">The FO synthase complex consists of two subunits, CofG and CofH.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the radical SAM superfamily. CofG family.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.32" evidence="2 10"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322916.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW29"/>
    <dbReference type="STRING" id="348780.NP_2398A"/>
    <dbReference type="EnsemblBacteria" id="CAI49290">
        <property type="protein sequence ID" value="CAI49290"/>
        <property type="gene ID" value="NP_2398A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703247"/>
    <dbReference type="KEGG" id="nph:NP_2398A"/>
    <dbReference type="eggNOG" id="arCOG00657">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054174_0_0_2"/>
    <dbReference type="OrthoDB" id="35347at2157"/>
    <dbReference type="UniPathway" id="UPA00072"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044689">
        <property type="term" value="F:7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01611">
        <property type="entry name" value="FO_synth_sub1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019939">
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        <property type="entry name" value="F420"/>
    </dbReference>
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        <property type="entry name" value="rSAM"/>
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        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03550">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PRK06245.1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="7,8-DIDEMETHYL-8-HYDROXY-5-DEAZARIBOFLAVIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FO SYNTHASE (COFH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00294">
        <property type="entry name" value="7_8-didemethyl-8-hydroxy-5-dea"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="7_8-didemethyl-8-hydroxy-5-dea"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="F420__menaquinone_cofactor_bio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Elp3"/>
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    </dbReference>
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        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6 10" id="KW-0408">Iron</keyword>
    <keyword evidence="7 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Radical SAM core" evidence="11">
        <location>
            <begin position="35"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI49290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="361" mass="39443" checksum="EA117DE03F84AE23" modified="2017-05-10" version="1">MFGAADYGVDIQVDDGDTERLLSVTPDDVSAAESLSFCRNVFVPLTTACRYTCTYCTYYEPPGQASLLSPAEIRDICERGADAGCTEALFTFGDDPDDRYERIHGQLAEWGHDSIHDYLREACDIALDCGLLPHSNPGDQTREQMGRVADLNASVGVMLETTAEVRAHGGPRAKEPGQRLATIATAGELGVPFTTGILVGIGETARDRAESILAIRELHERYGHIQEIIVQPVSNNDRWQQGSPELETLRRATAMARAGLPAEISVQSPPNLAPVRELLDCGVDDLGGVSPVTDDHVNPDYAWPALRELENIAEEAGVPLEERLPVQDRYAASEWLSPRIQSAIEADSEAGRRYRRVLESV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW36</accession>
    <name>A0A1U7EW36_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2338A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
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    <dbReference type="EnsemblBacteria" id="CAI49260">
        <property type="protein sequence ID" value="CAI49260"/>
        <property type="gene ID" value="NP_2338A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701412"/>
    <dbReference type="KEGG" id="nph:NP_2338A"/>
    <dbReference type="HOGENOM" id="CLU_1399779_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="194" mass="20247" checksum="EA95A23B447C5E15" modified="2017-05-10" version="1">MKRTTLILTLTVVLATTLAGAAAAPALAEETNDDYVELTFDEVDDGIVVEAVDTNTDHDVTNVDDELPDIDLSGLENGTTVTFEWDAVDADELQIEDEDGDVLETDDAEELSDDTGTLEVDWNDSLDDDADAYHFDNAALYDDDEDAVGTIGGSFFGGGVGDIGDDIDNTSLGIGAVGALVLVVGGIALAGRAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW77</accession>
    <name>A0A1U7EW77_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cupin 2 barrel domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2476A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322955.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW77"/>
    <dbReference type="STRING" id="348780.NP_2476A"/>
    <dbReference type="EnsemblBacteria" id="CAI49329">
        <property type="protein sequence ID" value="CAI49329"/>
        <property type="gene ID" value="NP_2476A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701457"/>
    <dbReference type="KEGG" id="nph:NP_2476A"/>
    <dbReference type="eggNOG" id="arCOG03006">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144575_1_0_2"/>
    <dbReference type="OrthoDB" id="305577at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051610">
        <property type="entry name" value="GPI/OXD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35848">
        <property type="entry name" value="OXALATE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35848:SF9">
        <property type="entry name" value="SLL1358 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin type-2" evidence="2">
        <location>
            <begin position="38"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="127" mass="14289" checksum="9AB4792DEBF1B259" modified="2017-05-10" version="1">MAEYKHVNYDDIEPVSGGMHFLREPLDSERVGVTVARCEPNWNSRPHDHADNDHEEIYVLIEGEATVVIDDEPVEMETGDAVWIPPEATRQIRNDDEECAFVLVSAPVSLEPDDGEHAEWTTDGFIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW92</accession>
    <name>A0A1U7EW92_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Archaeosine synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">arcS</name>
        <name evidence="5" type="synonym">tgtA2</name>
        <name evidence="5" type="ordered locus">NP_2506A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">tRNA modification; archaeosine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeosine synthase type 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322970.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW92"/>
    <dbReference type="STRING" id="348780.NP_2506A"/>
    <dbReference type="EnsemblBacteria" id="CAI49344">
        <property type="protein sequence ID" value="CAI49344"/>
        <property type="gene ID" value="NP_2506A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703242"/>
    <dbReference type="KEGG" id="nph:NP_2506A"/>
    <dbReference type="eggNOG" id="arCOG00990">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029831_0_0_2"/>
    <dbReference type="OrthoDB" id="115061at2157"/>
    <dbReference type="UniPathway" id="UPA00393"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002099">
        <property type="term" value="P:tRNA wobble guanine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21149">
        <property type="entry name" value="PUA_archaeosine_TGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.90">
        <property type="entry name" value="ArcTGT, C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.105">
        <property type="entry name" value="Queuine tRNA-ribosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10630">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053418">
        <property type="entry name" value="Archaeosine_synthase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050076">
        <property type="entry name" value="ArchSynthase1/Queuine_TRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040777">
        <property type="entry name" value="DUF5591"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
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        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036511">
        <property type="entry name" value="TGT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029402">
        <property type="entry name" value="TGT_C2"/>
    </dbReference>
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        <property type="entry name" value="TGT_C2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002616">
        <property type="entry name" value="tRNA_ribo_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040592">
        <property type="entry name" value="tRNA_mod_ArcS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46499:SF2">
        <property type="entry name" value="ARCHAEOSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46499">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17884">
        <property type="entry name" value="DUF5591"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01702">
        <property type="entry name" value="TGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14810">
        <property type="entry name" value="TGT_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88802">
        <property type="entry name" value="Pre-PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51713">
        <property type="entry name" value="tRNA-guanine transglycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="PUA" evidence="4">
        <location>
            <begin position="516"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="585" mass="63501" checksum="F41508953AF4471F" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWB3</accession>
    <name>A0A1U7EWB3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2568A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323000.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWB3"/>
    <dbReference type="EnsemblBacteria" id="CAI49375">
        <property type="protein sequence ID" value="CAI49375"/>
        <property type="gene ID" value="NP_2568A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701490"/>
    <dbReference type="KEGG" id="nph:NP_2568A"/>
    <dbReference type="eggNOG" id="arCOG04637">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_181982_0_0_2"/>
    <dbReference type="OrthoDB" id="311268at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055967">
        <property type="entry name" value="DUF7545"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24411">
        <property type="entry name" value="DUF7545"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="98" mass="10819" checksum="257E64BAC5B488BD" modified="2017-05-10" version="1">MADDTVTVTIETPDNRDEIEVVPEAIDVLNESDEGMPTVIGDLAMLGVAQQLHAVLHHAQGDIGVEESDANEQAMERTMELFEERFGQPFEEMMGHGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWD5</accession>
    <name>A0A1U7EWD5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Major facilitator superfamily transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">tp16</name>
        <name evidence="8" type="ordered locus">NP_2616A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323024.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWD5"/>
    <dbReference type="STRING" id="348780.NP_2616A"/>
    <dbReference type="EnsemblBacteria" id="CAI49399">
        <property type="protein sequence ID" value="CAI49399"/>
        <property type="gene ID" value="NP_2616A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703292"/>
    <dbReference type="KEGG" id="nph:NP_2616A"/>
    <dbReference type="eggNOG" id="arCOG00130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_5_14_2"/>
    <dbReference type="OrthoDB" id="204820at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050189">
        <property type="entry name" value="MFS_Efflux_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43124:SF3">
        <property type="entry name" value="CHLORAMPHENICOL EFFLUX PUMP RV0191"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43124">
        <property type="entry name" value="PURINE EFFLUX PUMP PBUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="133"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="163"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="211"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="242"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="276"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="298"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="337"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="362"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="9"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="390" mass="40811" checksum="FF65BC0EAED17406" modified="2017-05-10" version="1">MTPDYRHTLLFSCTVAFFATVTARLVISPVVPDIVDAFSTSNSAVGLALTGMWAAYALTQFPSGILGDRFGERRVILFAVVGTAVCSGLLALSPTFWLFALLALCLGVAAGLHYIVATALLTRNFERTGRAIGFHVTGAPLAGLLAPAAAALVATWYGWRAAVLVGAAVAVPAAALFAATVRPEPPQFPDRRMRDRVELGSIVRLYRRGPILYATVLSVLGAFTWQATASFLPAFLEAHHGLSRAVAGGLFSVYFFIHGALQPLMGSLSDRFSRDAIAAVTMGLGVVGYGSVVVGDSFSLVVAATVCVGVAMTWGAPLQSKFIDVLGEQERGAGFGLVRTVYMLLGALGSVAIGVVADLFGWPVAFGSLAGIMALGLLCILWNNVFGAGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWI9</accession>
    <name>A0A1U7EWI9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">UPF0058 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2722A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323077.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWI9"/>
    <dbReference type="STRING" id="348780.NP_2722A"/>
    <dbReference type="EnsemblBacteria" id="CAI49452">
        <property type="protein sequence ID" value="CAI49452"/>
        <property type="gene ID" value="NP_2722A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701537"/>
    <dbReference type="KEGG" id="nph:NP_2722A"/>
    <dbReference type="eggNOG" id="arCOG02254">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_167318_1_0_2"/>
    <dbReference type="OrthoDB" id="177623at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.110">
        <property type="entry name" value="Uncharacterised protein family UPF0058"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002753">
        <property type="entry name" value="UPF0058"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036519">
        <property type="entry name" value="UPF0058_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42203">
        <property type="entry name" value="UPF0058 PROTEIN MJ1205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42203:SF2">
        <property type="entry name" value="UPF0058 PROTEIN MJ1205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01893">
        <property type="entry name" value="UPF0058"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140371">
        <property type="entry name" value="Vng1086c-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="92" mass="10446" checksum="AE9E3DD2B6448AF8" modified="2017-05-10" version="1">MHKDELLELHAKMVSIMEHFREMDEIDASVFDGYDELDVTPDDVHKSKSEHKHAVFVLGNALAEVMAEDEFSDAGRIGKRMQELADDAESKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWJ7</accession>
    <name>A0A1U7EWJ7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HEWD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2692A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323062.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWJ7"/>
    <dbReference type="STRING" id="348780.NP_2692A"/>
    <dbReference type="EnsemblBacteria" id="CAI49437">
        <property type="protein sequence ID" value="CAI49437"/>
        <property type="gene ID" value="NP_2692A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701526"/>
    <dbReference type="KEGG" id="nph:NP_2692A"/>
    <dbReference type="eggNOG" id="arCOG04576">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_196451_0_0_2"/>
    <dbReference type="OrthoDB" id="212207at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046782">
        <property type="entry name" value="HEWD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20576">
        <property type="entry name" value="HEWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEWD" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="68" mass="7625" checksum="43FE1D2955CEF575" modified="2017-05-10" version="1">MSAEIVPPTKRTCERCGRTDVWDEAAQNWVIADVDGERQVGNKHCIHEWDINGQYNPIEGKQNTGADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWM5</accession>
    <name>A0A1U7EWM5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DUF304 domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2782A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323107.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWM5"/>
    <dbReference type="STRING" id="348780.NP_2782A"/>
    <dbReference type="EnsemblBacteria" id="CAI49482">
        <property type="protein sequence ID" value="CAI49482"/>
        <property type="gene ID" value="NP_2782A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701556"/>
    <dbReference type="KEGG" id="nph:NP_2782A"/>
    <dbReference type="eggNOG" id="arCOG04622">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104197_2_1_2"/>
    <dbReference type="OrthoDB" id="301911at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005182">
        <property type="entry name" value="YdbS-like_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34473:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34473">
        <property type="entry name" value="UPF0699 TRANSMEMBRANE PROTEIN YDBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03703">
        <property type="entry name" value="bPH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="YdbS-like PH" evidence="2">
        <location>
            <begin position="96"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="188" mass="20298" checksum="60876B36DB43FC68" modified="2017-05-10" version="1">MAESPFDESLTPAVRRVDTGLTPDDIPDIEELTGPPQTLEPEIQAVWSLQAAITAAILGGVAAVGFSMIEFGSPRIGGAVFTVGFLVGIVFAVLRYRIWTYQVREDSLYLHRGVLTQVTTVAPYVRIQHVDTRRSPTERVFGLSTTVVYTAGSRGADVSIPGLTPERAEDLQSRLKQLAIAAEGEDAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWP8</accession>
    <name>A0A1U7EWP8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type transport system permease protein (Probable substrate zinc)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">znuB1</name>
        <name evidence="7" type="synonym">abc19p</name>
        <name evidence="7" type="ordered locus">NP_2866A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC-3 integral membrane protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323149.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWP8"/>
    <dbReference type="STRING" id="348780.NP_2866A"/>
    <dbReference type="EnsemblBacteria" id="CAI49524">
        <property type="protein sequence ID" value="CAI49524"/>
        <property type="gene ID" value="NP_2866A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702424"/>
    <dbReference type="KEGG" id="nph:NP_2866A"/>
    <dbReference type="eggNOG" id="arCOG01006">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028808_3_1_2"/>
    <dbReference type="OrthoDB" id="241719at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="entry name" value="ABC transporter involved in vitamin B12 uptake, BtuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001626">
        <property type="entry name" value="ABC_TroCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30477">
        <property type="entry name" value="ABC-TRANSPORTER METAL-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30477:SF0">
        <property type="entry name" value="METAL TRANSPORT SYSTEM MEMBRANE PROTEIN TM_0125-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00950">
        <property type="entry name" value="ABC-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="ABC transporter involved in vitamin B12 uptake, BtuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="217"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="273"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="317"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="343"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="371" mass="38726" checksum="5429B3410FA6EF10" modified="2017-05-10" version="1">MSSETDTATDAPTDTEYSRPFRETVELVGVAAAGVLAFCMLAFIALDWLRGAPGALGVAGEIAFEQILIYGSWIDFYLGTDVFSYPSILRAVATGTLVGIVAPLVGTYLVHRQMALIGETLAHTAFAGVAVGVLVVAILGGTQAPIVEAVGSRGTLLLVALIVSAAGALGLQWLSDHTSTYGDVPIAIVLTGSFAVGTLLISWSRDFVAIAIDIEDFLFGNLAIVTATGARLVAILSVFVVALTVVHYKQFLFITFDERAARVARLNVDRYNALLVVMTAVVVVGAMQILGVILVAGLLVIPVAAASQVADSFRETLYLSVLVGQLSVLGGLAFAIWQSLPPGGSIIVIAIGFYLLAILFSGRDSGAISVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWR3</accession>
    <name>A0A1U7EWR3_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">acetate--CoA ligase (ADP-forming)</fullName>
            <ecNumber evidence="2">6.2.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">acdA</name>
        <name evidence="7" type="ordered locus">NP_2858A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetate + ATP + CoA = acetyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15081"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.13"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="6.2.1.13" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323145.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWR3"/>
    <dbReference type="STRING" id="348780.NP_2858A"/>
    <dbReference type="EnsemblBacteria" id="CAI49520">
        <property type="protein sequence ID" value="CAI49520"/>
        <property type="gene ID" value="NP_2858A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702472"/>
    <dbReference type="KEGG" id="nph:NP_2858A"/>
    <dbReference type="eggNOG" id="arCOG01340">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007415_3_1_2"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Protein lysine acetyltransferase"/>
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    </dbReference>
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        <location>
            <begin position="7"/>
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        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00001619"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="697" mass="72143" checksum="A1EE1A25B66365A0" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWS1</accession>
    <name>A0A1U7EWS1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2916A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="94" mass="9479" checksum="D4D0637DAD6B3CB4" modified="2017-05-10" version="1">MADSMSRMMRLLAGLARTALTFAVLVLLGIVAFYVTVFVVSTGAGLAGYDPSGDFVVLSASLLVVAALLGGIPLSAAASEANDGGDSRPRAGFE</sequence>
</entry>
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        </submittedName>
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    <gene>
        <name evidence="4" type="primary">hyuB1</name>
        <name evidence="4" type="ordered locus">NP_2952A</name>
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    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
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            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.2.14" evidence="4"/>
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        <property type="protein sequence ID" value="CAI49567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWT5"/>
    <dbReference type="STRING" id="348780.NP_2952A"/>
    <dbReference type="EnsemblBacteria" id="CAI49567">
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        <property type="gene ID" value="NP_2952A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702855"/>
    <dbReference type="KEGG" id="nph:NP_2952A"/>
    <dbReference type="eggNOG" id="arCOG01512">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020413_1_0_2"/>
    <dbReference type="OrthoDB" id="8261at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017168">
        <property type="term" value="F:5-oxoprolinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047423">
        <property type="term" value="F:N-methylhydantoinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003692">
        <property type="entry name" value="Hydantoinase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="5-OXOPROLINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365:SF23">
        <property type="entry name" value="HYPOTHETICAL 5-OXOPROLINASE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Hydantoinase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydantoinase B/oxoprolinase" evidence="3">
        <location>
            <begin position="11"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="464"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="497"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="176"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="531"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="540" mass="56835" checksum="7C34E8DD7780AFE4" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWZ1</accession>
    <name>A0A1U7EWZ1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable secreted glycoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3032A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323230.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWZ1"/>
    <dbReference type="EnsemblBacteria" id="CAI49607">
        <property type="protein sequence ID" value="CAI49607"/>
        <property type="gene ID" value="NP_3032A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701634"/>
    <dbReference type="KEGG" id="nph:NP_3032A"/>
    <dbReference type="eggNOG" id="arCOG02507">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_623514_0_0_2"/>
    <dbReference type="OrthoDB" id="204180at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051200">
        <property type="entry name" value="Host-pathogen_enzymatic-act"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011045">
        <property type="entry name" value="N2O_reductase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47197:SF3">
        <property type="entry name" value="DIHYDRO-HEME D1 DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47197">
        <property type="entry name" value="PROTEIN NIRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50974">
        <property type="entry name" value="Nitrous oxide reductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="409"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="439" mass="48219" checksum="B284D009CFAA0A26" modified="2017-05-10" version="1">MKRTTRRRFIASGAAVGAITIAGCADEDPEPEPEETPEDDETPTETEEEEADVFEAGYEIWAADQGTDTVYIYEPADDSGEEYDLLDEIDTGAEGGHVPHMADYSSDYEYVAIPCTTGARTLIYRAEDRELVANLETGAGSHFAGFGPNDEYIHVDVIGESEIVRVDVDLENEEFEIVDEIEVLEDETVQEAGIESGSPICHSYDKSGRSIHTMGPAYDDGSLVIVDHEEFEVDTAFPHEELPTNCGTMPHVTEDTFYLTAGNPTPTDDDGNVIEEQEDEAVGEYYVLDTENDEVIHNESTRGVDAHGFWFTPDGEELWVLNRETNDGVVVDPDTHEVVDEIDAFGPATAPDPDERDAPDIFWGSPDGEYMFVTLRGPEPQSGDPHAATGVNPGFAVIDTDTHDIVTTVQPDPDNDESDLHGIGVRPTREYDGFNLPPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWZ8</accession>
    <name>A0A1U7EWZ8_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">site-specific DNA-methyltransferase (adenine-specific)</fullName>
            <ecNumber evidence="2">2.1.1.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">NP_3112A</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N(6)-methyl-2'-deoxyadenosine in DNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15197"/>
            <dbReference type="Rhea" id="RHEA-COMP:12418"/>
            <dbReference type="Rhea" id="RHEA-COMP:12419"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90615"/>
            <dbReference type="ChEBI" id="CHEBI:90616"/>
            <dbReference type="EC" id="2.1.1.72"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the N(4)/N(6)-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.72" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323269.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWZ8"/>
    <dbReference type="STRING" id="348780.NP_3112A"/>
    <dbReference type="EnsemblBacteria" id="CAI49647">
        <property type="protein sequence ID" value="CAI49647"/>
        <property type="gene ID" value="NP_3112A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701657"/>
    <dbReference type="KEGG" id="nph:NP_3112A"/>
    <dbReference type="eggNOG" id="arCOG02635">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG03521">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_265794_0_0_2"/>
    <dbReference type="OrthoDB" id="237331at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009007">
        <property type="term" value="F:site-specific DNA-methyltransferase (adenine-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003356">
        <property type="entry name" value="DNA_methylase_A-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011639">
        <property type="entry name" value="MethylTrfase_TaqI-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050953">
        <property type="entry name" value="N4_N6_ade-DNA_methylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025931">
        <property type="entry name" value="TaqI_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33841:SF5">
        <property type="entry name" value="DNA METHYLASE (MODIFICATION METHYLASE) (METHYLTRANSFERASE)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33841">
        <property type="entry name" value="DNA METHYLTRANSFERASE YEEA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07669">
        <property type="entry name" value="Eco57I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02384">
        <property type="entry name" value="N6_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12950">
        <property type="entry name" value="TaqI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00507">
        <property type="entry name" value="N12N6MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 13" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0680">Restriction system</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA methylase adenine-specific" evidence="10">
        <location>
            <begin position="396"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="Type II methyltransferase M.TaqI-like" evidence="11">
        <location>
            <begin position="487"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="domain" description="TaqI-like C-terminal specificity" evidence="12">
        <location>
            <begin position="931"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="1207"/>
            <end position="1246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF12950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAI49647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="1258" mass="143854" checksum="585670EA27A4D168" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX14</accession>
    <name>A0A1U7EX14_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7 8">Chorismate synthase</fullName>
            <shortName evidence="7">CS</shortName>
            <ecNumber evidence="3 7 8">4.2.3.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">5-enolpyruvylshikimate-3-phosphate phospholyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 11" type="primary">aroC</name>
        <name evidence="11" type="ordered locus">NP_3082A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 proR hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate, which is the branch point compound that serves as the starting substrate for the three terminal pathways of aromatic amino acid biosynthesis. This reaction introduces a second double bond into the aromatic ring system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 9">
            <text>5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21020"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="EC" id="4.2.3.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7 9">
            <name>FMNH2</name>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
        </cofactor>
        <text evidence="7 9">Reduced FMN (FMNH(2)).</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 7 9">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 7/7.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 9">Belongs to the chorismate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.5" evidence="3 7 8"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323254.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX14"/>
    <dbReference type="STRING" id="348780.NP_3082A"/>
    <dbReference type="EnsemblBacteria" id="CAI49632">
        <property type="protein sequence ID" value="CAI49632"/>
        <property type="gene ID" value="NP_3082A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702527"/>
    <dbReference type="KEGG" id="nph:NP_3082A"/>
    <dbReference type="eggNOG" id="arCOG04133">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034547_0_0_2"/>
    <dbReference type="OrthoDB" id="33049at2157"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00090"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004107">
        <property type="term" value="F:chorismate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07304">
        <property type="entry name" value="Chorismate_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.150.10">
        <property type="entry name" value="Chorismate synthase AroC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00300">
        <property type="entry name" value="Chorismate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000453">
        <property type="entry name" value="Chorismate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035904">
        <property type="entry name" value="Chorismate_synth_AroC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020541">
        <property type="entry name" value="Chorismate_synthase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00033">
        <property type="entry name" value="aroC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003793">
        <property type="entry name" value="PRK05382.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21085">
        <property type="entry name" value="CHORISMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21085:SF0">
        <property type="entry name" value="CHORISMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01264">
        <property type="entry name" value="Chorismate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001456">
        <property type="entry name" value="Chorismate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103263">
        <property type="entry name" value="Chorismate synthase, AroC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00787">
        <property type="entry name" value="CHORISMATE_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00788">
        <property type="entry name" value="CHORISMATE_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00789">
        <property type="entry name" value="CHORISMATE_SYNTHASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 7 9" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0274">FAD</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0288">FMN</keyword>
    <keyword evidence="6 7 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="258"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="373"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="125"/>
            <end position="127"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="312"/>
            <end position="316"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="NCBIfam" id="TIGR00033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI49632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="395" mass="42889" checksum="F499A652FC46695D" modified="2017-05-10" version="1">MNGNSFGRLFQVTTYGESHGDAMGVTVSGCPAGVELDEEAIQYELDRRKPGQSKITTSRGEPDEVTINSGTLEGYTTGTPIGMVIQNKDARSGKYEPFITAPRPSHGDFTYSAKFGTRNWGGGGRSSARETVNWVAAGAVAKQVLEQSEHDVEIKAHVNQIGDIEAPPVSFEEMVEHSEENEVRCGHPETAARMRERIDEYQEAGDSIGGAIEFEVRGVPRGLGAPRFDSFEARLGKAMLSVPATTAFELGLGREAREYTGSERNDEWAFDEGDRPEAVSDEGDPVPVENDHGGLQGGITTGLPIYGEVTLHAPTSIPKGQQTADWETDEIVDDAQVIGRHDPVLPPRGVPVVEAMLYLTVLDFMLLGGRINPDRIDDSPGEYDTDYHPSSPRNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX81</accession>
    <name>A0A1U7EX81_NATPD</name>
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        <submittedName>
            <fullName evidence="3">Prephenate dehydrogenase</fullName>
            <ecNumber evidence="3">1.3.1.12</ecNumber>
        </submittedName>
    </protein>
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        <name evidence="3" type="primary">tyrA</name>
        <name evidence="3" type="ordered locus">NP_3358A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.3.1.12" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323390.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX81"/>
    <dbReference type="STRING" id="348780.NP_3358A"/>
    <dbReference type="EnsemblBacteria" id="CAI49770">
        <property type="protein sequence ID" value="CAI49770"/>
        <property type="gene ID" value="NP_3358A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703431"/>
    <dbReference type="KEGG" id="nph:NP_3358A"/>
    <dbReference type="eggNOG" id="arCOG00245">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077586_0_0_2"/>
    <dbReference type="OrthoDB" id="24743at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008977">
        <property type="term" value="F:prephenate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004665">
        <property type="term" value="F:prephenate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006571">
        <property type="term" value="P:tyrosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046826">
        <property type="entry name" value="PDH_N"/>
    </dbReference>
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        <property type="entry name" value="Preph/Arog_dehydrog"/>
    </dbReference>
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        <property type="entry name" value="Prephen_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21363">
        <property type="entry name" value="PREPHENATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21363:SF0">
        <property type="entry name" value="PREPHENATE DEHYDROGENASE [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
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    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51176">
        <property type="entry name" value="PDH_ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prephenate/arogenate dehydrogenase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="243" mass="25341" checksum="F0135F99F6BE9503" modified="2017-05-10" version="1">MQLLVVGAGEMGQWFARHSGADAVAFADRDSDTARRAAAGLNDAQAVPLDTDERFDVVCIAVPMSAVADAVAAHAGKATAAVVDVAGEMRDSVAALRKHAPDRERASFHPLFSASNAPGNLPVVVDAEGPTVAALRAALSAAGNEVFETTPEEHDRAMETVQAQAHTAVLAYALAADDVDSRFHTALSEPLEALVDDVVGNTPEVYAEIQSRFDGSDAVAEAAQRLADADASEFADLYEEAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX86</accession>
    <name>A0A1U7EX86_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">AstE domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">NP_3404A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323413.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX86"/>
    <dbReference type="STRING" id="348780.NP_3404A"/>
    <dbReference type="EnsemblBacteria" id="CAI49793">
        <property type="protein sequence ID" value="CAI49793"/>
        <property type="gene ID" value="NP_3404A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701766"/>
    <dbReference type="KEGG" id="nph:NP_3404A"/>
    <dbReference type="eggNOG" id="arCOG02888">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1064042_0_0_2"/>
    <dbReference type="OrthoDB" id="323389at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050178">
        <property type="entry name" value="AspA/AstE_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055438">
        <property type="entry name" value="AstE_AspA_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15162">
        <property type="entry name" value="ASPARTOACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15162:SF7">
        <property type="entry name" value="SUCCINYLGLUTAMATE DESUCCINYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24827">
        <property type="entry name" value="AstE_AspA_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Succinylglutamate desuccinylase/Aspartoacylase catalytic" evidence="5">
        <location>
            <begin position="11"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI49793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="279" mass="30076" checksum="F4CD3CBEE920A9D9" modified="2017-05-10" version="1">MRVETLGDGEPEVAIVGGIHGDEPCGPAAVEALLDAAPDVERPVKLVVANEKALARGVRYVDEDLNRAFPGDPDADTHEGRLAHELLGEIRGCEILSLHSTQSYPEPFALVDERDGYARSVCSYLSVEALVETAQYSGGRLIAYPGVVELECGLQRSTAAAKNAEALAREFLVAVGALSGGGEQTRHHPLPIFRLDKQIPKPEADTYEVFVENFERVAEGDAFAAADGERLVAEWPFYPVLLSAYGYRNVFGYAGELVGRLGDETPTRHGAVEADGRED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX92</accession>
    <name>A0A1U7EX92_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">CBS domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cbs4</name>
        <name evidence="4" type="ordered locus">NP_3394A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323408.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX92"/>
    <dbReference type="STRING" id="348780.NP_3394A"/>
    <dbReference type="EnsemblBacteria" id="CAI49788">
        <property type="protein sequence ID" value="CAI49788"/>
        <property type="gene ID" value="NP_3394A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702582"/>
    <dbReference type="KEGG" id="nph:NP_3394A"/>
    <dbReference type="eggNOG" id="arCOG00606">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040681_12_2_2"/>
    <dbReference type="OrthoDB" id="43333at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd17776">
        <property type="entry name" value="CBS_pair_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="7"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="72"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="150" mass="16810" checksum="F2ED55FAFADAF2AA" modified="2017-05-10" version="1">MLVKEAMTERVVTCHANVSLRTAAGRMLRNDVGSVIVRDDETPVGMVTETDALRAGFAADVAFSEIPVSKVMNRPLVTITKDKTLRRATERMEEENIKKLPVVEDFDLVGVLTAHDVIEHYHDLRKEISTVTRPGATRPTESERFSFEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXR3</accession>
    <name>A0A1U7EXR3_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF8045 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3840A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323628.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXR3"/>
    <dbReference type="STRING" id="348780.NP_3840A"/>
    <dbReference type="EnsemblBacteria" id="CAI50011">
        <property type="protein sequence ID" value="CAI50011"/>
        <property type="gene ID" value="NP_3840A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701881"/>
    <dbReference type="KEGG" id="nph:NP_3840A"/>
    <dbReference type="eggNOG" id="arCOG09172">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_935732_0_0_2"/>
    <dbReference type="OrthoDB" id="190808at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058358">
        <property type="entry name" value="DUF8045"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26193">
        <property type="entry name" value="DUF8045"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8045" evidence="1">
        <location>
            <begin position="3"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="297" mass="33154" checksum="75319ACD60CD8C47" modified="2017-05-10" version="1">MKLRYYADAPNYDDHEQIIDLLYTISDKYGITVEIERVNNRYGSIQVFPGDIRENSPEDVYDRDFHYNRTLGSNIDESPSQAFKASGRHVDIDGYVGIIDDGLVWATTNRGDQIGYGPDVDPTDTTLGFLDQAANHGLEAIEEKYMDEDERERTVIEQFLTADVVDGTVHRDVVVGTSQLPDSPAHGVDSSVGEIVTRTVDAIIETDESDWIVQTAKTFEASAFDTALGQILVRDRLYRFDTGTNTDTTLAIVFNTVPWELDIDAVPATVDQLTAISDGPDVRVFAGRDREFKEVTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY03</accession>
    <name>A0A1U7EY03_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4014A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50098.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323714.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY03"/>
    <dbReference type="STRING" id="348780.NP_4014A"/>
    <dbReference type="EnsemblBacteria" id="CAI50098">
        <property type="protein sequence ID" value="CAI50098"/>
        <property type="gene ID" value="NP_4014A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701940"/>
    <dbReference type="KEGG" id="nph:NP_4014A"/>
    <dbReference type="eggNOG" id="arCOG07777">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_999714_0_0_2"/>
    <dbReference type="OrthoDB" id="238714at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055685">
        <property type="entry name" value="DUF7261"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23922">
        <property type="entry name" value="DUF7261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50098.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="287" mass="30718" checksum="B93E611129C25BAA" modified="2017-05-10" version="1">MAEVTDRDRGQLLLVAGIVLAVLFVALALLVNTAIYTDNVATRDGDAASEPLAYQEGAVSVASGLIEAENEDGDDFGTIESNVRDGFEESLSMLEQRHLQRAATTETGTSGFTEGRHIQQSDGIDNWGVSGADGVRAFSMTLERGSMDSIDIDDEDDPDPFNIDLGDGELEVYLDAENDDDLTVSVDGEVRCRVDASGDVSFDVTDERLDGKPCPLGLSSDLDDDFVGFDNADTVDGEYELIVATTSEPAAPEVSEVLYSVVLDVGIHSPDRSYESRVTVAPGEFDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY23</accession>
    <name>A0A1U7EY23_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DUF790 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4066A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323740.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY23"/>
    <dbReference type="STRING" id="348780.NP_4066A"/>
    <dbReference type="EnsemblBacteria" id="CAI50124">
        <property type="protein sequence ID" value="CAI50124"/>
        <property type="gene ID" value="NP_4066A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701957"/>
    <dbReference type="KEGG" id="nph:NP_4066A"/>
    <dbReference type="eggNOG" id="arCOG04356">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038336_0_0_2"/>
    <dbReference type="OrthoDB" id="57367at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008508">
        <property type="entry name" value="Bax1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39640:SF1">
        <property type="entry name" value="DUF790 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39640">
        <property type="entry name" value="VNG6129C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05626">
        <property type="entry name" value="DUF790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019435">
        <property type="entry name" value="UCP019435"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="499" mass="54926" checksum="A91004E4C39B17A1" modified="2017-05-10" version="1">MLTKELLRVSRAGGGYHPQFTTAADEDVAARVLGVYQGHVGEPRAQLESALTDLERETGQFKLVRGFAKLLEREAAFETRSPVSPRRARTAAFVAAESVGVVTEADRDAALVAAADDLDTSADALAASLFADRDCEQRLTAFDVDYDPESLCTQYDLSLAQTALFDATSVRVRTDDPKRLVSAVKRLRLLYEIRRADAGRDVVVTGPDALFKRSRRYGTRFARLLRTVAATADWQLEATIDDRGTERRLRLEAGDIEVPDTDPVAEPTFDSAVEADFYSRFSSLDLDWRLLREPEPLAAGEHVVIPDFAFEWKHGDFRVFFEIMGFWTPEYVEKKLSRFSDLEDVAFLVAYDESLGVGEDIEATGQRAFPYTKTVRLKDVRDALRPYEETLREASAASLPDRLVPDADVTTLAAVADDHGVSEAVVESATFPEHDRVGRTLVRPAVCDAVDEELTAGMSLAEAETVLSGHGIDETSAMLSALGYRVEWEGLGGGTVTEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY50</accession>
    <name>A0A1U7EY50_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ADP-ribose pyrophosphatase</fullName>
            <ecNumber evidence="2">3.6.1.13</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4100A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.1.13" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323757.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY50"/>
    <dbReference type="STRING" id="348780.NP_4100A"/>
    <dbReference type="EnsemblBacteria" id="CAI50141">
        <property type="protein sequence ID" value="CAI50141"/>
        <property type="gene ID" value="NP_4100A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702606"/>
    <dbReference type="KEGG" id="nph:NP_4100A"/>
    <dbReference type="eggNOG" id="arCOG00215">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030805_0_2_2"/>
    <dbReference type="OrthoDB" id="372037at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047631">
        <property type="term" value="F:ADP-ribose diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00885">
        <property type="entry name" value="cinA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050101">
        <property type="entry name" value="CinA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056596">
        <property type="entry name" value="FLAD1_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13939">
        <property type="entry name" value="NICOTINAMIDE-NUCLEOTIDE AMIDOHYDROLASE PNCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13939:SF0">
        <property type="entry name" value="NMN AMIDOHYDROLASE-LIKE PROTEIN YFAY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24102">
        <property type="entry name" value="FLAD1_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoaB/Mog" evidence="1">
        <location>
            <begin position="4"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="231" mass="24538" checksum="599CF2EC836AB3F2" modified="2017-05-10" version="1">MNVALVSVGDELLSGDTVNTNAAWLGERLTERGASVARVTVVPDRVADIARVVNEHAADYDAVIVTGGIGPTHDDKTMEGVAAAVGRSLSRNEAALEWLESAGGYAREDLTEGTTLLPADAEPLHNTEGVAPGCKVENVYVLPGVPAEMKAMFESVADDFDGEPPHVGYVAVNEPESQLVDRLQELQERFAVTVGSYPGEHVRVKIESTDAAAVNAAMAWLQDRAEVEESD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY85</accession>
    <name>A0A1U7EY85_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF8072 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_4186A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323800.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY85"/>
    <dbReference type="EnsemblBacteria" id="CAI50184">
        <property type="protein sequence ID" value="CAI50184"/>
        <property type="gene ID" value="NP_4186A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701998"/>
    <dbReference type="KEGG" id="nph:NP_4186A"/>
    <dbReference type="eggNOG" id="arCOG07593">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170069_0_0_2"/>
    <dbReference type="OrthoDB" id="339988at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058385">
        <property type="entry name" value="DUF8072"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26269">
        <property type="entry name" value="DUF8072"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8072" evidence="2">
        <location>
            <begin position="2"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="87" mass="9431" checksum="D83B4DD5388E7740" modified="2017-05-10" version="1">MHTLAKRFHNITPDRILVTFEDGSSVVVEPRSAEFFQEAFQAEGVADDGTVYRFVTDGGDDPLVAGEQTDDGWTPAGTVSDVEPADE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY88</accession>
    <name>A0A1U7EY88_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Acyl-CoA synthetase</fullName>
            <ecNumber evidence="5">6.2.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">acs10</name>
        <name evidence="5" type="ordered locus">NP_4150A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.-" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323782.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY88"/>
    <dbReference type="STRING" id="348780.NP_4150A"/>
    <dbReference type="EnsemblBacteria" id="CAI50166">
        <property type="protein sequence ID" value="CAI50166"/>
        <property type="gene ID" value="NP_4150A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702506"/>
    <dbReference type="KEGG" id="nph:NP_4150A"/>
    <dbReference type="eggNOG" id="arCOG00856">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_5_2"/>
    <dbReference type="OrthoDB" id="193284at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016877">
        <property type="term" value="F:ligase activity, forming carbon-sulfur bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12119">
        <property type="entry name" value="ttLC_FACS_AlkK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000008">
        <property type="entry name" value="2,3-dihydroxybenzoate-AMP ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050237">
        <property type="entry name" value="ATP-dep_AMP-bd_enzyme"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004837">
        <property type="entry name" value="PRK06187.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43767">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43767:SF11">
        <property type="entry name" value="MEDIUM-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="3">
        <location>
            <begin position="15"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="4">
        <location>
            <begin position="443"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI50166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="549" mass="61612" checksum="61A6291F61E096EE" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYA9</accession>
    <name>A0A1U7EYA9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acyl-CoA synthetase</fullName>
            <ecNumber evidence="4">6.2.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">acs7</name>
        <name evidence="4" type="ordered locus">NP_4256A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323835.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYA9"/>
    <dbReference type="STRING" id="348780.NP_4256A"/>
    <dbReference type="EnsemblBacteria" id="CAI50219">
        <property type="protein sequence ID" value="CAI50219"/>
        <property type="gene ID" value="NP_4256A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702503"/>
    <dbReference type="KEGG" id="nph:NP_4256A"/>
    <dbReference type="eggNOG" id="arCOG00856">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_0_2"/>
    <dbReference type="OrthoDB" id="275266at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016878">
        <property type="term" value="F:acid-thiol ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050237">
        <property type="entry name" value="ATP-dep_AMP-bd_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43767">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43767:SF1">
        <property type="entry name" value="NONRIBOSOMAL PEPTIDE SYNTHASE PES1 (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="2">
        <location>
            <begin position="15"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="3">
        <location>
            <begin position="433"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="506"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="515"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="533" mass="58503" checksum="F61D4CBBCF358630" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYD9</accession>
    <name>A0A1U7EYD9_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Amidophosphoribosyltransferase</fullName>
            <shortName evidence="7">ATase</shortName>
            <ecNumber evidence="7">2.4.2.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Glutamine phosphoribosylpyrophosphate amidotransferase</fullName>
            <shortName evidence="7">GPATase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 14" type="primary">purF</name>
        <name evidence="14" type="ordered locus">NP_4312A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + L-glutamine + H2O</text>
            <dbReference type="Rhea" id="RHEA:14905"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="EC" id="2.4.2.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7 10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="7 10">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7 11">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="7 11">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 7 8">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 8">In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.14" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323863.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYD9"/>
    <dbReference type="STRING" id="348780.NP_4312A"/>
    <dbReference type="EnsemblBacteria" id="CAI50247">
        <property type="protein sequence ID" value="CAI50247"/>
        <property type="gene ID" value="NP_4312A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703071"/>
    <dbReference type="KEGG" id="nph:NP_4312A"/>
    <dbReference type="eggNOG" id="arCOG00093">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022389_3_1_2"/>
    <dbReference type="OrthoDB" id="5976at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00124"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004044">
        <property type="term" value="F:amidophosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00715">
        <property type="entry name" value="GPATase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01931">
        <property type="entry name" value="PurF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005854">
        <property type="entry name" value="PurF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035584">
        <property type="entry name" value="PurF_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01134">
        <property type="entry name" value="purF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11907">
        <property type="entry name" value="AMIDOPHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13522">
        <property type="entry name" value="GATase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000485">
        <property type="entry name" value="Amd_phspho_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6 7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3 7 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 11" id="KW-0408">Iron</keyword>
    <keyword evidence="7 11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="13">
        <location>
            <begin position="14"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="465"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7 9">
        <location>
            <position position="14"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 11">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 10">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 10">
        <location>
            <position position="366"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 10">
        <location>
            <position position="367"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 11">
        <location>
            <position position="403"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 11">
        <location>
            <position position="454"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 11">
        <location>
            <position position="457"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAI50247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="494" mass="53234" checksum="489E7AFE34A7A872" modified="2017-05-10" version="1">MPDGRHSDEPTEKCGVVGVSLDGRDAARPLYYALYALQHRGQESAGIVTHDGFQQHSHVEMGLVGDAFEEGDLDSLNGPTGIGHVRYPTAGSVNSSCAQPFSVSFKSGSLGLSHNGNLVNADELRQDLAASGHAFTSDGDTEVIAHDLARNLLEEDLIRAVKRTMNRIHGSYSLTITHDDTVLGVRDPQGNRPLCIGELDDGYILASESAAIDVLDGEFVRDVNPGELVVLDDDGGGYDSYQLVDAENSAHCFFEHVYFARPDSRIDSKLVYDVRRELGRKLWEEAGVESDVVVPVPDSGRAFASGYAEAAQEDGTNVEFAEGLMKNRYVGRTFIMPTQDARERAVRLKLNPIKDVIEGKTVTVIDDSIVRGTTSTQLVELLKDVGAESVHMRIGAPAIVAPCYMGIDMASRDELIAAGRDVEEIREEIAADSLSYLSVDAVAEALGESEDDLCLGCVTGEYPYDIEGEPTDRDIARPEVGGDQPEAGVAGSDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYE2</accession>
    <name>A0A1U7EYE2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="13">P-type transport ATPase (Probable substrate copper/metal cation)</fullName>
            <ecNumber evidence="13">3.6.3.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">tpa05</name>
        <name evidence="13" type="ordered locus">NP_4300A</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.-" evidence="13"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323857.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYE2"/>
    <dbReference type="STRING" id="348780.NP_4300A"/>
    <dbReference type="EnsemblBacteria" id="CAI50241">
        <property type="protein sequence ID" value="CAI50241"/>
        <property type="gene ID" value="NP_4300A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703352"/>
    <dbReference type="KEGG" id="nph:NP_4300A"/>
    <dbReference type="eggNOG" id="arCOG01576">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001771_0_3_2"/>
    <dbReference type="OrthoDB" id="8588at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043682">
        <property type="term" value="F:P-type divalent copper transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055070">
        <property type="term" value="P:copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520">
        <property type="entry name" value="ATP7, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520:SF8">
        <property type="entry name" value="P-TYPE CU(+) TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01229">
        <property type="entry name" value="COF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
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    </dbReference>
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    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="153"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="232"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="256"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="407"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="434"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="769"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="12">
        <location>
            <begin position="64"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="539"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="539"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAI50241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="812" mass="84838" checksum="85060B6CF5FFD892" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYF2</accession>
    <name>A0A1U7EYF2_NATPD</name>
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        <submittedName>
            <fullName evidence="2">DUF357 family protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">NP_4324A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
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            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="STRING" id="348780.NP_4324A"/>
    <dbReference type="EnsemblBacteria" id="CAI50253">
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        <property type="gene ID" value="NP_4324A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702031"/>
    <dbReference type="KEGG" id="nph:NP_4324A"/>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_180506_0_0_2"/>
    <dbReference type="OrthoDB" id="148073at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="DUF357"/>
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    <dbReference type="Pfam" id="PF04010">
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF158372">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF357" evidence="1">
        <location>
            <begin position="11"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="94" mass="10480" checksum="75F3B891B51D2793" modified="2017-05-10" version="1">MSADLEEKTDRYERLLAEALAAAEIAPQEGTPLYEAAVEFREMAQSYLEDGRHFRDNDDPVNALASFSYGHGWMDAGARVGIFEVPTDGHLFTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYG1</accession>
    <name>A0A1U7EYG1_NATPD</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    </protein>
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        <name evidence="2" type="ordered locus">NP_4352A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
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            <taxon>Halobacteriales</taxon>
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            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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    <dbReference type="STRING" id="348780.NP_4352A"/>
    <dbReference type="EnsemblBacteria" id="CAI50267">
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        <property type="gene ID" value="NP_4352A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702039"/>
    <dbReference type="KEGG" id="nph:NP_4352A"/>
    <dbReference type="HOGENOM" id="CLU_1631703_0_0_2"/>
    <dbReference type="OrthoDB" id="242154at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="162" mass="15912" checksum="6B7F707844A95661" modified="2017-05-10" version="1">MRQHLFGRTPRLTLYFCGLAVGLSLLVAGVAAARLSVGGGAPGVGWPAVAVGTAGGLVLAFVAGYLNGGLLASWACGLVPTAGRLGVPLAEGTWLDVAVTLVGSAGYGVLLGAAGFVVAVEKHRRDARTADLPAPPSRTAVVGLTALSGVLGAILVGFGAVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYH2</accession>
    <name>A0A1U7EYH2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Probable F420-dependent oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_4374A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYH2"/>
    <dbReference type="STRING" id="348780.NP_4374A"/>
    <dbReference type="EnsemblBacteria" id="CAI50278">
        <property type="protein sequence ID" value="CAI50278"/>
        <property type="gene ID" value="NP_4374A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702043"/>
    <dbReference type="KEGG" id="nph:NP_4374A"/>
    <dbReference type="eggNOG" id="arCOG02410">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027853_5_0_2"/>
    <dbReference type="OrthoDB" id="194060at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01097">
        <property type="entry name" value="Tetrahydromethanopterin_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="entry name" value="Luciferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050564">
        <property type="entry name" value="F420-G6PD/mer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023909">
        <property type="entry name" value="F420_NP1902A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04024">
        <property type="entry name" value="F420_NP1902A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43244">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43244:SF1">
        <property type="entry name" value="5,10-METHYLENETETRAHYDROMETHANOPTERIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="Bacterial luciferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Luciferase-like" evidence="2">
        <location>
            <begin position="13"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="333" mass="36023" checksum="0E49762066A4C6BA" modified="2017-05-10" version="1">MVDRDIVLPSGLDSLDDVVAVAQRAESLGYDRLSLPEVTGRDGVTLLATLAARTDRIGLSNDVFSPWGRSPAMLGQTGVTVQEASGGRYRMGLGTSSPNLTEGWHLAAYERPLRRLRETIDIIRQVTAGEELDYDGDHFDGGGLRLRGVEPRSVPVDVAALGPKAVELAGRFADGWVPQLFTVAALEDRLEDLRRGAALGDRSTDDLRVAVTVRSCALEDASRARTAARKQLAFMVGAYGPYYRQSIARQGFEAATDDIHGAWTDGDRQRAIEAVTEEMVDSLVAAGTPEAVRETVRRYEAVDGVDAVRIGYFGDMDADERRQTTNALAPSDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYN6</accession>
    <name>A0A1U7EYN6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">siroheme decarboxylase</fullName>
            <ecNumber evidence="4">4.1.1.111</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ahbB</name>
        <name evidence="8" type="synonym">nirGH</name>
        <name evidence="8" type="ordered locus">NP_4498A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>siroheme + 2 H(+) = 12,18-didecarboxysiroheme + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:19093"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:60052"/>
            <dbReference type="ChEBI" id="CHEBI:140497"/>
            <dbReference type="EC" id="4.1.1.111"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Porphyrin-containing compound metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Ahb/Nir family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.111" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323955.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYN6"/>
    <dbReference type="STRING" id="348780.NP_4498A"/>
    <dbReference type="EnsemblBacteria" id="CAI50340">
        <property type="protein sequence ID" value="CAI50340"/>
        <property type="gene ID" value="NP_4498A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702081"/>
    <dbReference type="KEGG" id="nph:NP_4498A"/>
    <dbReference type="eggNOG" id="arCOG01628">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049427_0_0_2"/>
    <dbReference type="OrthoDB" id="145939at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3460">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040523">
        <property type="entry name" value="AsnC_trans_reg2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050684">
        <property type="entry name" value="HTH-Siroheme_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053953">
        <property type="entry name" value="NirdL-like_HTH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413:SF1">
        <property type="entry name" value="SIROHEME DECARBOXYLASE NIRL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ASNC FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17805">
        <property type="entry name" value="AsnC_trans_reg2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22451">
        <property type="entry name" value="NirdL-like_HTH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Siroheme decarboxylase NirL-like HTH" evidence="7">
        <location>
            <begin position="11"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Siroheme decarboxylase AsnC-like ligand binding" evidence="6">
        <location>
            <begin position="76"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Siroheme decarboxylase NirL-like HTH" evidence="7">
        <location>
            <begin position="189"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Siroheme decarboxylase AsnC-like ligand binding" evidence="6">
        <location>
            <begin position="246"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF17805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF22451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI50340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="352" mass="39280" checksum="36833C77A426A0CB" modified="2017-05-10" version="1">MSETAGLDRLDRAVINAYQGGFPVVESPFEPAAEALADHGVDVTATELLKRVQTLDEEGVLTRFGALINAAEIGGAATLVAMHAPEDRFEAVAEQVNSHREVAHNYRREHPHLNMWFVVSVADADAVERVLADIEDETGQETYNLPKQQEFRVEAKFLVDGPIPDGDVDLSDLGPDASPTDRGTLTPEERDLVVEIQDGLPVTETPYADVAAELGVDTDWVLRTIQRFNESGKIRRVGVIPNHYALGYTENGMTVWSVPDEKLDEVGPEVAGLDFVTHCYERPRHEGVWPYNFFAMTHGRSEDESKRRIQQVKSTMEEYWDVGDDDWDSLFSTEILKKTGIRLDERATANTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYP4</accession>
    <name>A0A1U7EYP4_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Aspartate kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_4532A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50357.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323972.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYP4"/>
    <dbReference type="STRING" id="348780.NP_4532A"/>
    <dbReference type="EnsemblBacteria" id="CAI50357">
        <property type="protein sequence ID" value="CAI50357"/>
        <property type="gene ID" value="NP_4532A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702090"/>
    <dbReference type="KEGG" id="nph:NP_4532A"/>
    <dbReference type="eggNOG" id="arCOG01449">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079807_0_0_2"/>
    <dbReference type="OrthoDB" id="197035at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056678">
        <property type="entry name" value="DUF7776"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046260">
        <property type="entry name" value="HFX_2341-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24989">
        <property type="entry name" value="DUF7776"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19810">
        <property type="entry name" value="HFX_2341_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HFX-2341-like N-terminal" evidence="1">
        <location>
            <begin position="2"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7776" evidence="2">
        <location>
            <begin position="140"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50357.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="298" mass="33915" checksum="3E2461DA8A63FA41" modified="2017-05-10" version="1">MQTHIVPVGFDYDRLIAPLVRDQLDVDRVILLEGAVGSEANVEYSRNLADKLQKDFTNLLGAETEEYLVEDVYDYDAAFEQAYDLINGELDTGSEVWVNVSAMPRTVSFAFATAAHSIMVEREGDRDRIHTYYTVPEKYLETELAEELRAQCSLLEDVLEDDVEDERIEEHLDVARDLLEEFDERGTTIGAKQFGDSHIIELPVASFSNVKPFEELILFTLGEHDEFESVSELAQTLARELGEEYTDSFRSKVIYNVDRLGPGGKGYIEQESHGKSYRTRLSRIGELWVRSHADESDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYS2</accession>
    <name>A0A1U7EYS2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_4644A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324027.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYS2"/>
    <dbReference type="STRING" id="348780.NP_4644A"/>
    <dbReference type="EnsemblBacteria" id="CAI50413">
        <property type="protein sequence ID" value="CAI50413"/>
        <property type="gene ID" value="NP_4644A"/>
    </dbReference>
    <dbReference type="GeneID" id="69102744"/>
    <dbReference type="KEGG" id="nph:NP_4644A"/>
    <dbReference type="eggNOG" id="arCOG06437">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073251_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055693">
        <property type="entry name" value="DUF7269"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23933">
        <property type="entry name" value="DUF7269"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="229" mass="25584" checksum="B6E431A7469B6D2D" modified="2017-05-10" version="1">MSRFSTIINKLLVGIALFAFVFAIILSVQPSLLPSAVLDSFLTVENEANHNYVLLGIATVVALFALWRMCFPTSGSLDAEDGRGGAAASVYDHEYDAGIVGERMDRRIEQALESLKQGQRSESNVETVIDDMRKTLLAVEKSKGRSDAAASERVQCGDWTDDQVVAVFLGDAPAGRLSFRHRVEMWLFPERTWRRRLERTVAELEQYATESREWTAEGERGESDGNQTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYS9</accession>
    <name>A0A1U7EYS9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4604A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324008.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYS9"/>
    <dbReference type="EnsemblBacteria" id="CAI50393">
        <property type="protein sequence ID" value="CAI50393"/>
        <property type="gene ID" value="NP_4604A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702112"/>
    <dbReference type="KEGG" id="nph:NP_4604A"/>
    <dbReference type="eggNOG" id="arCOG07582">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_193442_0_0_2"/>
    <dbReference type="OrthoDB" id="200060at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055964">
        <property type="entry name" value="DUF7542"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24398">
        <property type="entry name" value="DUF7542"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="82" mass="8728" checksum="EB4B2DCB60E0B067" modified="2017-05-10" version="1">MSDERVAVACRDCEYTASFSSLGRARTALAAHETETGHDIDWDIDHAAEGVERAGADAGVCGSPDCANADSPLLDWRESTDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYT1</accession>
    <name>A0A1U7EYT1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Probable secreted glycoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">NP_4622A</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="18">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Cell wall</location>
            <location evidence="2">S-layer</location>
        </subcellularLocation>
    </comment>
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        <text evidence="3">Belongs to the halobacterial S-layer protein family.</text>
    </comment>
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        <property type="protein sequence ID" value="CAI50402.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="NP_4622A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702121"/>
    <dbReference type="KEGG" id="nph:NP_4622A"/>
    <dbReference type="eggNOG" id="arCOG06273">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006819_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="1022"/>
            <end position="1041"/>
        </location>
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        <location>
            <begin position="467"/>
            <end position="571"/>
        </location>
    </feature>
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        <location>
            <begin position="1023"/>
            <end position="1043"/>
        </location>
    </feature>
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        <location>
            <begin position="836"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="966"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="839"/>
            <end position="854"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="974"/>
            <end position="1020"/>
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        <source>
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        <source>
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        <source>
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            <dbReference type="Pfam" id="PF18204"/>
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        <source>
            <dbReference type="Pfam" id="PF25162"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI50402.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
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    <sequence length="1049" mass="114134" checksum="AFC4D8F6E47D0E2C" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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            <taxon>Halobacteriales</taxon>
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            <taxon>Natronomonas</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <location>
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            <end position="295"/>
        </location>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI50403.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="1323" mass="140389" checksum="2ACC0C77457051E4" modified="2017-05-10" version="1"> </sequence>
</entry>
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    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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            <authorList>
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
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            </authorList>
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            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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        <property type="gene ID" value="NP_4766A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703433"/>
    <dbReference type="KEGG" id="nph:NP_4766A"/>
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    </dbReference>
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        <property type="entry name" value="Prenyltransferase_related"/>
    </dbReference>
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        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="28"/>
            <end position="44"/>
        </location>
    </feature>
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        <location>
            <begin position="50"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
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        <location>
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            <end position="259"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="265"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI50474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="290" mass="30807" checksum="F616E5883DB9C635" modified="2017-05-10" version="1">MSQPLFDRLLPSDATAAGYLLRLSRPRFWLYLAGPVVVGVAYAAETPAELFSPLAVALFAYFLLPANVFLYGVNDIFDADIDIENPKKEQREVRYRGDWTVPALVVATGALGVAFLPALPLAGVAAMVGFLFLAVEYSAPPLRFKTTPWLDSLSNGLYILPGVVAYAGIAGDGPPLAAIAAGWLWAMGMHTFSAIPDIDPDRAAGIETTATVLGARRTYAYCGGCWLAAAAVFGTVDVFFAAVLGIYPVFVAAIAGLGIDPERAYWWFPALNTAAGTALTLGALWVMLHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ11</accession>
    <name>A0A1U7EZ11_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_4790A</name>
    </gene>
    <organism evidence="3 4">
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        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
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            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
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            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50486.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_049939712.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ11"/>
    <dbReference type="STRING" id="348780.NP_4790A"/>
    <dbReference type="EnsemblBacteria" id="CAI50486">
        <property type="protein sequence ID" value="CAI50486"/>
        <property type="gene ID" value="NP_4790A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702176"/>
    <dbReference type="KEGG" id="nph:NP_4790A"/>
    <dbReference type="eggNOG" id="arCOG04907">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149107_0_0_2"/>
    <dbReference type="OrthoDB" id="341846at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040493">
        <property type="entry name" value="DUF5518"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17647">
        <property type="entry name" value="DUF5518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="79"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="118"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50486.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="145" mass="15197" checksum="53D70203DA2763B3" modified="2017-05-10" version="1">MVPDDSLRSDDTLSRDHSRHDDARQRDTPRDDGSSTVLNAVIGAVVGVVLYFIPLSTLLGGATTAYLDGGTRTDGAKAGALAGGFMFLPVAFFTLVGGLFFFPFLLGGPGLPVSLWPLLLGFSAVYTVGLAALGGYLGIYLKEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ93</accession>
    <name>A0A1U7EZ93_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Mrp-type sodium/proton antiporter system subunit D4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">mrpD4</name>
        <name evidence="9" type="ordered locus">NP_5056A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ93"/>
    <dbReference type="STRING" id="348780.NP_5056A"/>
    <dbReference type="EnsemblBacteria" id="CAI50619">
        <property type="protein sequence ID" value="CAI50619"/>
        <property type="gene ID" value="NP_5056A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_5056A"/>
    <dbReference type="eggNOG" id="arCOG01542">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007100_11_2_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003945">
        <property type="entry name" value="NU5C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001516">
        <property type="entry name" value="Proton_antipo_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42829:SF1">
        <property type="entry name" value="INORGANIC CARBON TRANSPORTER SUBUNIT DABB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42829">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00662">
        <property type="entry name" value="Proton_antipo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01434">
        <property type="entry name" value="NADHDHGNASE5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="106"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="263"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="304"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="354"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="382"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="417"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="457"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="NADH-Ubiquinone oxidoreductase (complex I) chain 5 N-terminal" evidence="8">
        <location>
            <begin position="69"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:quinone oxidoreductase/Mrp antiporter transmembrane" evidence="7">
        <location>
            <begin position="123"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI50619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="511" mass="53728" checksum="AAD440801FF906DF" modified="2017-05-10" version="1">MARAGSGRNMSSKPSPPGDATAQRSERPPSSSLVPRVSTWTVWALFLASVGVLAVAVWRGVGWGVAGILHIDGLTAVMWVVVTFFSGIVHSYSRRYMDGDRYIDRFFGKVFAFTVVVGVLVAADHVAVFVAAWLAMGLAMASLIGHVRDWEQARAAGRLARRYFLGSSLLLVGALAPLVWMTDATTITGLLAAFDTVPRTVAIAAAGGIFLAAIVQSALLPFHGWLLSSMTAPTPASALMHAGFVNAGGVLLTRFAPVIADELVFMSVVVLVGATSALLGQAMLLVQTDIKQKLGSSTLAQMGFMIMQCGLGFFAAAIAHLILHGCYKAYLFLASGATVEQTTPKKSGHGHTSLGFPSLAISLLTAIGGGVLFMALTGKLAGLTVTLDSGIVLTLVVVLTTLTAARDILHQASLPTAVRFVGVPLVVLVAIGSYAVLFNAISGMLSGVPMTQTPTELTAIHYLVVALFAGAYLAVELGYHRSSERLYVALLNLSQPDSETVLTNKEDYHDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZB1</accession>
    <name>A0A1U7EZB1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription elongation factor Spt4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2 4" type="primary">spt4</name>
        <name evidence="4" type="synonym">rpoE2</name>
        <name evidence="4" type="ordered locus">NP_5078A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Stimulates transcription elongation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer composed of Spt4 and Spt5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal Spt4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324240.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZB1"/>
    <dbReference type="STRING" id="348780.NP_5078A"/>
    <dbReference type="EnsemblBacteria" id="CAI50630">
        <property type="protein sequence ID" value="CAI50630"/>
        <property type="gene ID" value="NP_5078A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703156"/>
    <dbReference type="KEGG" id="nph:NP_5078A"/>
    <dbReference type="eggNOG" id="arCOG04077">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_199467_0_0_2"/>
    <dbReference type="OrthoDB" id="275101at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00949">
        <property type="entry name" value="Spt4_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029040">
        <property type="entry name" value="RPABC4/Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022800">
        <property type="entry name" value="Spt4/RpoE2_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007178">
        <property type="entry name" value="Spt4_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038589">
        <property type="entry name" value="Spt4_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041664">
        <property type="entry name" value="RNAP_arch_Epp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40704">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40704:SF1">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06093">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01389">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63393">
        <property type="entry name" value="RNA polymerase subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Spt4/RpoE2 zinc finger" evidence="3">
        <location>
            <begin position="4"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="65" mass="7228" checksum="1F7D3C462705BBA5" modified="2017-05-10" version="1">MADRLVCRDCHRVLEVDDGEQCPSCGSTSMTEDWAGYVVVAHPEDSRIAEEMGISEPGRYALKVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZE6</accession>
    <name>A0A1U7EZE6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Type IV pilus biogenesis complex ATPase subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_5204A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GSP E family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324303.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZE6"/>
    <dbReference type="STRING" id="348780.NP_5204A"/>
    <dbReference type="EnsemblBacteria" id="CAI50693">
        <property type="protein sequence ID" value="CAI50693"/>
        <property type="gene ID" value="NP_5204A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702293"/>
    <dbReference type="KEGG" id="nph:NP_5204A"/>
    <dbReference type="eggNOG" id="arCOG01818">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017428_1_0_2"/>
    <dbReference type="OrthoDB" id="31341at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050921">
        <property type="entry name" value="T4SS_GSP_E_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30486">
        <property type="entry name" value="TWITCHING MOTILITY PROTEIN PILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30486:SF6">
        <property type="entry name" value="TYPE IV PILUS RETRACTATION ATPASE PILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bacterial type II secretion system protein E" evidence="2">
        <location>
            <begin position="260"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="592" mass="62128" checksum="B36DBC4547FBEF8B" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZG0</accession>
    <name>A0A1U7EZG0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF373 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5162A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324282.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZG0"/>
    <dbReference type="STRING" id="348780.NP_5162A"/>
    <dbReference type="EnsemblBacteria" id="CAI50672">
        <property type="protein sequence ID" value="CAI50672"/>
        <property type="gene ID" value="NP_5162A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702277"/>
    <dbReference type="KEGG" id="nph:NP_5162A"/>
    <dbReference type="eggNOG" id="arCOG04151">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048986_1_0_2"/>
    <dbReference type="OrthoDB" id="201773at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007254">
        <property type="entry name" value="DUF373"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38815:SF1">
        <property type="entry name" value="DUF373 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38815">
        <property type="entry name" value="HYPOTHETICAL MEMBRANE PROTEIN, CONSERVED, DUF373 FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04123">
        <property type="entry name" value="DUF373"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="219"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="263"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="296"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="324"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="366" mass="38486" checksum="2FF01B79E3950DED" modified="2017-05-10" version="1">MLLILCVDLDDDLGRKTDFETPVVGRDAVEEAAVALATADPEDSDVNVCFQGVHLYDTIDDESVEVAVVTGTAKADVSANRKVGEEVDTVLASLSTGEDVRAVVVTDGAQDESVLPVIRSRVPIDGVRRVVVRQAQDLESMYYTFKQVLADPETRGTILIPLGILLLIYPVAIVADMLGLPGSTLGVISALLGLYILFRGLGLESTIDEALEQVRVGLFAGRVQLVTYVVAAALVAVGIVGGVETAGELTTVDPEAVSATAAFVYGAVEWVAAAGVIASLGRVTDEYLSGGFRWRYLNAPFYVVAIAVVLHGLSAFFLELDFAGISYLAATLTVGTLVGLASTLAFAVAEQRYPLADAESESELAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZG2</accession>
    <name>A0A1U7EZG2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7310 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_5200A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324301.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZG2"/>
    <dbReference type="STRING" id="348780.NP_5200A"/>
    <dbReference type="EnsemblBacteria" id="CAI50691">
        <property type="protein sequence ID" value="CAI50691"/>
        <property type="gene ID" value="NP_5200A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702291"/>
    <dbReference type="KEGG" id="nph:NP_5200A"/>
    <dbReference type="eggNOG" id="arCOG07564">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1976599_0_0_2"/>
    <dbReference type="OrthoDB" id="206571at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055734">
        <property type="entry name" value="DUF7310"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23991">
        <property type="entry name" value="DUF7310"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7310" evidence="2">
        <location>
            <begin position="11"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="126" mass="13920" checksum="3E245DFE47FD207A" modified="2017-05-10" version="1">MPPRDHDRDAIERRLEAVERALAEDEQPDRVDRLDELETRVAELEAAVRALRGYVGSVRAINDDVERRADRALRKAEAVERTVDPTEPSGDSAAGEGSAEDEADATEHAADAPTATGRLQRLRDHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZG3</accession>
    <name>A0A1U7EZG3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ParA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">parA1</name>
        <name evidence="2" type="ordered locus">NP_5240A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324321.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZG3"/>
    <dbReference type="STRING" id="348780.NP_5240A"/>
    <dbReference type="EnsemblBacteria" id="CAI50711">
        <property type="protein sequence ID" value="CAI50711"/>
        <property type="gene ID" value="NP_5240A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702308"/>
    <dbReference type="KEGG" id="nph:NP_5240A"/>
    <dbReference type="eggNOG" id="arCOG00589">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037612_0_3_2"/>
    <dbReference type="OrthoDB" id="267074at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051782">
        <property type="term" value="P:negative regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050625">
        <property type="entry name" value="ParA/MinD_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384:SF10">
        <property type="entry name" value="ATPASE INVOLVED IN CHROMOSOME PARTITIONING, PARA_MIND FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384">
        <property type="entry name" value="SEPTUM SITE-DETERMINING PROTEIN MIND HOMOLOG, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobQ/CobB/MinD/ParA nucleotide binding" evidence="1">
        <location>
            <begin position="5"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="254" mass="26010" checksum="098CC6D87938BB87" modified="2017-05-10" version="1">MVEAIAVASGKGGTGKTTATLALGMALAEEYDVTVVDADTGMANLLFHTGLGDAETTLHDVLVEGGAPVEAATYERFGMSVVPCGTSLAAFEAADPERLRDVVAALAADTDVILLDSPATLDSKSAVLPIVLADRVVVVLNPTVPAISDGLKVQEYALSYGTDIAGTVFNRVHGDIEDVRAKAERYFEGPTLATVPESDSARAARAAGEPLLAHAPADPAAEAFRAAAEGLSPADGDEGAVAERFRTAVIPDPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZG4</accession>
    <name>A0A1U7EZG4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">NamA family oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">NP_5226A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324314.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZG4"/>
    <dbReference type="STRING" id="348780.NP_5226A"/>
    <dbReference type="EnsemblBacteria" id="CAI50704">
        <property type="protein sequence ID" value="CAI50704"/>
        <property type="gene ID" value="NP_5226A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702303"/>
    <dbReference type="KEGG" id="nph:NP_5226A"/>
    <dbReference type="eggNOG" id="arCOG00615">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012153_2_0_2"/>
    <dbReference type="OrthoDB" id="122964at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003959">
        <property type="term" value="F:NADPH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02932">
        <property type="entry name" value="OYE_YqiM_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044152">
        <property type="entry name" value="YqjM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43303">
        <property type="entry name" value="NADPH DEHYDROGENASE C23G7.10C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43303:SF4">
        <property type="entry name" value="NADPH DEHYDROGENASE C23G7.10C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:flavin oxidoreductase/NADH oxidase N-terminal" evidence="7">
        <location>
            <begin position="2"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="104"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI50704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="357" mass="38730" checksum="3BA7244FBE494804" modified="2017-05-10" version="1">MDLFTPFDCRDVTARNRVMVSPMCQYSCEDEDGLATDWHLVHLGSRAAGGAGIVMSEATAVTPRGRISPKDLGIWSDDHADALAPVTEFIAEQGATSAIQLAHAGRKASTNRPGDGGGPVHGDRGWTPVGPTTEPWPHDEPLSTERLDAADIERITEAFTEAAVRAVDAGFDIVELHAAHGYLLHEFLSPVTNTREDDYGGSFENRTRFLREVVVSVREAIPEGTPLFVRISATDWLPDRESWTVDDSTRLAAELRPLGVDLIDVSAGAIHPDQQVPDTGPHYQVPYAEQVAESGVPVGTVGKITTPEGADEIIRNGRAALAIVGREHLRDPYFALHAAESLGYELPVPRQYTRGFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZM2</accession>
    <name>A0A1U7EZM2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 19 20">Adenosylcobinamide-GDP ribazoletransferase</fullName>
            <ecNumber evidence="5 19 20">2.7.8.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16 19">Cobalamin synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15 19">Cobalamin-5'-phosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19 21" type="primary">cobS</name>
        <name evidence="21" type="ordered locus">NP_5306A</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14 19">Joins adenosylcobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin). Also synthesizes adenosylcobalamin 5'-phosphate from adenosylcobinamide-GDP and alpha-ribazole 5'-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 19">
            <text>alpha-ribazole + adenosylcob(III)inamide-GDP = adenosylcob(III)alamin + GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16049"/>
            <dbReference type="ChEBI" id="CHEBI:10329"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:60487"/>
            <dbReference type="EC" id="2.7.8.26"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18 19">
            <text>alpha-ribazole 5'-phosphate + adenosylcob(III)inamide-GDP = adenosylcob(III)alamin 5'-phosphate + GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23560"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57918"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:60487"/>
            <dbReference type="ChEBI" id="CHEBI:60493"/>
            <dbReference type="EC" id="2.7.8.26"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 19">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 19">Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 7/7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 19">Cell membrane</location>
            <topology evidence="2 19">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 19">Belongs to the CobS family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.26" evidence="5 19 20"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324353.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZM2"/>
    <dbReference type="STRING" id="348780.NP_5306A"/>
    <dbReference type="EnsemblBacteria" id="CAI50744">
        <property type="protein sequence ID" value="CAI50744"/>
        <property type="gene ID" value="NP_5306A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702621"/>
    <dbReference type="KEGG" id="nph:NP_5306A"/>
    <dbReference type="eggNOG" id="arCOG04338">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057426_2_0_2"/>
    <dbReference type="OrthoDB" id="11748at2157"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00238"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051073">
        <property type="term" value="F:adenosylcobinamide-GDP ribazoletransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008818">
        <property type="term" value="F:cobalamin 5'-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00719">
        <property type="entry name" value="CobS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003805">
        <property type="entry name" value="CobS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00317">
        <property type="entry name" value="cobS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34148">
        <property type="entry name" value="ADENOSYLCOBINAMIDE-GDP RIBAZOLETRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34148:SF1">
        <property type="entry name" value="ADENOSYLCOBINAMIDE-GDP RIBAZOLETRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02654">
        <property type="entry name" value="CobS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 19" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 19" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="11 19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 19 21" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="63"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="136"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="181"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="NCBIfam" id="TIGR00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAI50744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="249" mass="24803" checksum="E9BB11DC17320B18" modified="2017-05-10" version="1">MAVNDLVGGLRGGVAFLSRLPVDSGEADWERFRTLPVAFPLVGYLIGALVALPFFLPAPSATVAFAYLLALAVVVGIPHFDGVADLGDAVAAHGPSSKRAALKDTDTGVGAIVAVAVVFLGLVLAALALSTAPLAVAVTVVIAAEVGAKLGMATVGCLGTAGHEGMGSQFTTVLDSEMLVVPALAAVPAAVAVLPATLAAVLAGPLVAVVLVDWADEQLGGVSGDIFGATNELARVAGLHLGVVAWTLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZP5</accession>
    <name>A0A1U7EZP5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3381D</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CCI69581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_148215449.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZP5"/>
    <dbReference type="STRING" id="348780.NP_3381D"/>
    <dbReference type="EnsemblBacteria" id="CCI69581">
        <property type="protein sequence ID" value="CCI69581"/>
        <property type="gene ID" value="NP_3381D"/>
    </dbReference>
    <dbReference type="GeneID" id="41345100"/>
    <dbReference type="KEGG" id="nph:NP_3381D"/>
    <dbReference type="HOGENOM" id="CLU_197998_0_0_2"/>
    <dbReference type="OrthoDB" id="240474at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058412">
        <property type="entry name" value="DUF8099"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26401">
        <property type="entry name" value="DUF8099"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCI69581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="61" mass="6742" checksum="EEA2ED32B6DB2AFC" modified="2017-05-10" version="1">MDSASPIDQRIAGETALDTGITFTFSMGFNKAENTLKRGFCGRPRGYRHIHHRSYGEGLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILX2</accession>
    <name>Q3ILX2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">O-acetyltransferase (Homolog to galactoside O-acetyltransferase)</fullName>
            <ecNumber evidence="4">2.3.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">maa2</name>
        <name evidence="4" type="ordered locus">NP_6236A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4 5">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324495.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILX2"/>
    <dbReference type="EnsemblBacteria" id="CAI50898">
        <property type="protein sequence ID" value="CAI50898"/>
        <property type="gene ID" value="NP_6236A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694596"/>
    <dbReference type="KEGG" id="nph:NP_6236A"/>
    <dbReference type="HOGENOM" id="CLU_065082_0_0_2"/>
    <dbReference type="OrthoDB" id="224761at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04647">
        <property type="entry name" value="LbH_MAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051159">
        <property type="entry name" value="Hexapeptide_acetyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23416:SF23">
        <property type="entry name" value="ACETYLTRANSFERASE C18B11.09C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23416">
        <property type="entry name" value="SIALIC ACID SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="257"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="299" mass="33105" checksum="5E6B0011365B276E" modified="2005-11-08" version="1">MTKRHVEVPVETQAAIDETIDYVDMRLSEDDTADAVAEILADLFGDSDVYEQYRAGESLPPMTRLRVRSYDPRNVLIETEHWAEQELSALQESKCLRYLWEGFDLSPLSNNLAFALPFRQTLADHLFAEAGDGLRLFSGIKIQCGHNIEMGDNVVVHNDVLLDDRGRLQIGDRVSVADRSHIHTHAHDTVDQSDVTNYETILDDDVRLGYGSMISAGCRIGENAMVGSGATTLGDVPPHHIAAGSPAKSVKIKPGWEAIADDPGPLVDNRERRQLPSDVPDSVDEFDEFNRNLSPPSES</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILY0</accession>
    <name>Q3ILY0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">N-methylhydantoinase (ATP-hydrolyzing) A</fullName>
            <ecNumber evidence="4">3.5.2.14</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hyuA2</name>
        <name evidence="4" type="ordered locus">NP_6218A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4 5">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.2.14" evidence="4"/>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324487.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILY0"/>
    <dbReference type="EnsemblBacteria" id="CAI50889">
        <property type="protein sequence ID" value="CAI50889"/>
        <property type="gene ID" value="NP_6218A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694594"/>
    <dbReference type="KEGG" id="nph:NP_6218A"/>
    <dbReference type="HOGENOM" id="CLU_002157_1_2_2"/>
    <dbReference type="OrthoDB" id="8261at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017168">
        <property type="term" value="F:5-oxoprolinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047423">
        <property type="term" value="F:N-methylhydantoinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049517">
        <property type="entry name" value="ACX-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008040">
        <property type="entry name" value="Hydant_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002821">
        <property type="entry name" value="Hydantoinase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="5-OXOPROLINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365:SF23">
        <property type="entry name" value="HYPOTHETICAL 5-OXOPROLINASE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19278">
        <property type="entry name" value="Hydant_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05378">
        <property type="entry name" value="Hydant_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01968">
        <property type="entry name" value="Hydantoinase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydantoinase/oxoprolinase N-terminal" evidence="2">
        <location>
            <begin position="5"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Hydantoinase A/oxoprolinase" evidence="1">
        <location>
            <begin position="206"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="Acetophenone carboxylase-like C-terminal" evidence="3">
        <location>
            <begin position="503"/>
            <end position="677"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="687" mass="73261" checksum="755C5A5E4A994B37" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="135" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILY5</accession>
    <name>Q3ILY5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ORC1-type DNA replication protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">orc5</name>
        <name evidence="8" type="synonym">cdc6E</name>
        <name evidence="8" type="ordered locus">NP_6208A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8 9">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in regulation of DNA replication.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the CDC6/cdc18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50884.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324482.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILY5"/>
    <dbReference type="EnsemblBacteria" id="CAI50884">
        <property type="protein sequence ID" value="CAI50884"/>
        <property type="gene ID" value="NP_6208A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694682"/>
    <dbReference type="KEGG" id="nph:NP_6208A"/>
    <dbReference type="HOGENOM" id="CLU_025112_2_0_2"/>
    <dbReference type="OrthoDB" id="195574at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08768">
        <property type="entry name" value="Cdc6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000073">
        <property type="entry name" value="ORC1-type DNA replication protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01407">
        <property type="entry name" value="ORC1_type_DNA_replic_protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015163">
        <property type="entry name" value="Cdc6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055237">
        <property type="entry name" value="Cdc6_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050311">
        <property type="entry name" value="ORC1/CDC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014277">
        <property type="entry name" value="Orc1/Cdc6_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02928">
        <property type="entry name" value="orc1/cdc6 family replication initiation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763:SF22">
        <property type="entry name" value="ORC1-TYPE DNA REPLICATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22703">
        <property type="entry name" value="Cdc6_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09079">
        <property type="entry name" value="WHD_Cdc6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="116"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="22"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="48"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="128"/>
            <end position="132"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI50884.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="489" mass="54443" checksum="E727B69C6084B64C" modified="2005-11-08" version="1">MGTDDSDASRPESRTPEDETTQADLSSSATSSNVPADVDHSTEAADGSPDAGSRSDESSSQSIEDMLLEFDEQDGLIRDRSLLDPNYVVDEDRIVGRDDQLQEVTKMLRVTLGDNRPPNLFLYGPSGTGKSLITKAVCNNINRICETRDIDFGTIEVNCQDLDTLGVAVYELATQAAEQAGVAVQVPKHGVATKEKWDELFRIVNENFDSVVFVLDELDMLVGRRDKQEPAFSRLLYQLSRAEATNDLRAYLSVVAISNDTKMMESVGSRALSSFTPEDVHFDDYDANQLQTILRHRQDAFHDGVVDDDVIPLAAAFAAQTHGDARKAIDLMRVAGELAEREGSTRVREEHVRQAQEKVEKNRVLEVVRGISTQKKLCLYATAAVAAQTDGGTARSTTGYRVYQFLTDSINADQYHQETYVNKMKELTTYSLVDFERRSHGPSSGMFLEFQFGERPETILETLREDSRIEMVTSEEVQSVVQAQIRKQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILZ4</accession>
    <name>Q3ILZ4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC-type transport system periplasmic substrate-binding protein (Probable substrate branched-chain amino acids)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">livJ2</name>
        <name evidence="3" type="synonym">abc06s</name>
        <name evidence="3" type="ordered locus">NP_6190A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50875.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_158303768.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILZ4"/>
    <dbReference type="EnsemblBacteria" id="CAI50875">
        <property type="protein sequence ID" value="CAI50875"/>
        <property type="gene ID" value="NP_6190A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694668"/>
    <dbReference type="KEGG" id="nph:NP_6190A"/>
    <dbReference type="HOGENOM" id="CLU_027128_4_1_2"/>
    <dbReference type="OrthoDB" id="162762at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="CDD" id="cd06338">
        <property type="entry name" value="PBP1_ABC_ligand_binding-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051010">
        <property type="entry name" value="BCAA_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028081">
        <property type="entry name" value="Leu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019546">
        <property type="entry name" value="TAT_signal_bac_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01409">
        <property type="entry name" value="TAT_signal_seq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30483:SF37">
        <property type="entry name" value="ABC TRANSPORTER SUBSTRATE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30483">
        <property type="entry name" value="LEUCINE-SPECIFIC-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13458">
        <property type="entry name" value="Peripla_BP_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Leucine-binding protein" evidence="2">
        <location>
            <begin position="51"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50875.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="433" mass="46637" checksum="1A9B9BD85E9F197C" modified="2012-10-03" version="2">MVNRNQPSTAESTDRRTFLKASGAMGAAGLAGLAGCLDGFDDTPEDRDVFRFGAVTSLSGDLRFGGTITERGYDLWEQAVNENGGIEIDGTSYEVEVEYADAQSEPSTGADAAEQMIDSGVDALFGPYSSNVTLAVAPIAEREGLPHITGSAESPDIWKEQYRYTFGTIPAGNVIAGDAAESIFNFDPAPETVCITGVNDPFTSDVAEALRETAEANGIEVLYYELFPRDADWTSPVSVAKNEDPDLHFHAAHIEAHVDFLTAAKDLDYNPDAFFSHYGVDTESFAEGLGDDAAHTLGATVWLPRLDLPGDILFDGPQDYADASEAAFDVTPDYTQAGSTAAGIVYQEALQELGAAPPLLDDEKDELIDILENIEVQTFYGEVDFETEGDLYHSNTAADILTIQRSTDGFDIVAPDTQMESEPEYPVPEWGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM00</accession>
    <name>Q3IM00_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">IS1341-type transposase ISNph17</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">NP_6178A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8 9">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324467.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM00"/>
    <dbReference type="EnsemblBacteria" id="CAI50869">
        <property type="protein sequence ID" value="CAI50869"/>
        <property type="gene ID" value="NP_6178A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694637"/>
    <dbReference type="KEGG" id="nph:NP_6178A"/>
    <dbReference type="HOGENOM" id="CLU_040997_0_0_2"/>
    <dbReference type="OrthoDB" id="295419at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="6">
        <location>
            <begin position="201"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="7">
        <location>
            <begin position="340"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="413"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="443"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="448"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI50869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="466" mass="53514" checksum="B5EE0D7FF26EF90B" modified="2005-11-08" version="1">MSDRPQRTNTYTANATSDRYRQCLFDWLAAQPPLWNQITYRRRQAYFSDDGDVWDADYTDLYDEYAPIIGKATCQQIARKNSEAWRSHFRLLEQYHDNSNTTVTEKPSPPGYWGNRKEGYELHGLVRNDLYTLDWDEDRSTLEFGVGDVLKDRYDFEHNERVTLEIKGNPQWNGDDSRLELIYDDHADQLRVQHPVRIQPDKIREQREDAFTHTLNTENTTQAAAIDVGANNTLAVVTERGDTAVYHARPEFARFQRYSERIATLQSGLPADKYTSSLIQRLYDTRSRSRDHSRDAAVKHVAEWLLERNVDTVYVGDLTDVLDTHWSTDVNEKTHNFWSHRQLLNRIELTLGDVGICVAEVTEADSSSECPECGSSDITRRGDSFRCHDCELDAHSDVAGAWNILQSEAGPMARPAALSAERDRDAPQEGAYWQWNEHDWIPADFGEQSRSVDQPSFSKPASSQPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM18</accession>
    <name>Q3IM18_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_6138A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324449.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM18"/>
    <dbReference type="EnsemblBacteria" id="CAI50849">
        <property type="protein sequence ID" value="CAI50849"/>
        <property type="gene ID" value="NP_6138A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694621"/>
    <dbReference type="KEGG" id="nph:NP_6138A"/>
    <dbReference type="HOGENOM" id="CLU_2299420_0_0_2"/>
    <dbReference type="OrthoDB" id="381610at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="37"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="100" mass="11341" checksum="49C79F9DC3EC428E" modified="2005-11-08" version="1">MSEDTGEETDGESVAERLDTVEEQIDKLGFENIDSRVKTIESEQAELRADLEELVERIRSEGEETPHIDSVEMLYERLGTLETNIATLRDSLEEVVKDGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM32</accession>
    <name>Q3IM32_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Lrp/AsnC family transcription regulator / TrkA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">trkA3</name>
        <name evidence="6" type="ordered locus">NP_6106A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6 7">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324435.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM32"/>
    <dbReference type="EnsemblBacteria" id="CAI50833">
        <property type="protein sequence ID" value="CAI50833"/>
        <property type="gene ID" value="NP_6106A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694608"/>
    <dbReference type="KEGG" id="nph:NP_6106A"/>
    <dbReference type="HOGENOM" id="CLU_1088218_0_0_2"/>
    <dbReference type="OrthoDB" id="6762at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043200">
        <property type="term" value="P:response to amino acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="entry name" value="Regulator of K+ conductance, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154">
        <property type="entry name" value="LEUCINE-RESPONSIVE REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154:SF34">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR AZLB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="TrkA C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="10"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="5">
        <location>
            <begin position="164"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI50833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="257" mass="28318" checksum="6D2C00CA74D371E7" modified="2005-11-08" version="1">MSDTGSEHRLDQIDRRIIYALMGDARNTSAPDIAEHLSVSGATVRNRIARLEERGIIKDYQATIDFEQADGSLMNLYLCHAPFGEVEAVSRKLGTVPGVINVRELMGGRRNLHVLAVGRDTDDLRRIGREIEELDIEIEDEFLLQQELHFPYLPYGPEESRPGKPLADYMSLAGGAEVVELTVEESAPIVGCTLEEATQKNIIEEQTLVIAIERDDTVITPKGDTEIQPQDVVTVFSPGGAGELVAEGFRSPPDHEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM71</accession>
    <name>Q3IM71_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF8158 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_6016A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324396.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM71"/>
    <dbReference type="EnsemblBacteria" id="CAI50788">
        <property type="protein sequence ID" value="CAI50788"/>
        <property type="gene ID" value="NP_6016A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694582"/>
    <dbReference type="KEGG" id="nph:NP_6016A"/>
    <dbReference type="HOGENOM" id="CLU_2204034_0_0_2"/>
    <dbReference type="OrthoDB" id="191541at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058471">
        <property type="entry name" value="DUF8158"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26488">
        <property type="entry name" value="DUF8158"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8158" evidence="2">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="109" mass="12233" checksum="4FAD7C42F71298B3" modified="2005-11-08" version="1">MPDTEPSYDVREQTGDPDHASVDDVVDLVIHRAQAPRTDHEDAHFDRTMATVIDTYGTDPVRTVIHRILVDDEPFRTATNGLEMRNVDGVRIGTAASWFLKELNAQDSD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IME1</accession>
    <name>ARLY_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Argininosuccinate lyase</fullName>
            <shortName evidence="1">ASAL</shortName>
            <ecNumber evidence="1">4.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Arginosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argH</name>
        <name type="ordered locus">NP_5254A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-(N(omega)-L-arginino)succinate = fumarate + L-arginine</text>
            <dbReference type="Rhea" id="RHEA:24020"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="EC" id="4.3.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.1" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324327.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IME1"/>
    <dbReference type="SMR" id="Q3IME1"/>
    <dbReference type="STRING" id="348780.NP_5254A"/>
    <dbReference type="EnsemblBacteria" id="CAI50718">
        <property type="protein sequence ID" value="CAI50718"/>
        <property type="gene ID" value="NP_5254A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702522"/>
    <dbReference type="KEGG" id="nph:NP_5254A"/>
    <dbReference type="eggNOG" id="arCOG01748">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027272_2_3_2"/>
    <dbReference type="OrthoDB" id="27337at2157"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00114"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004056">
        <property type="term" value="F:argininosuccinate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01359">
        <property type="entry name" value="Argininosuccinate_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.200.10:FF:000015">
        <property type="entry name" value="argininosuccinate lyase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.40.30">
        <property type="entry name" value="Fumarase/aspartase (C-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00006">
        <property type="entry name" value="Arg_succ_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029419">
        <property type="entry name" value="Arg_succ_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009049">
        <property type="entry name" value="Argininosuccinate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00838">
        <property type="entry name" value="argH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814">
        <property type="entry name" value="ARGININOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814:SF1">
        <property type="entry name" value="ARGININOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14698">
        <property type="entry name" value="ASL_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00145">
        <property type="entry name" value="ARGSUCLYASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000240793" description="Argininosuccinate lyase">
        <location>
            <begin position="1"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="426"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="469"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="440"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="469"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="490" mass="51179" checksum="BB64A365C42509A9" modified="2005-11-08" version="1">MTGDADEGRDVVRRDRFSGGPARGFLSSLAADERIFEADLAVDRAHVVMLDEQDIIGTDDAAAILGALDDVEAAGHGSLTDGEDVHAAIETAVIERVGDRGGKMHTARSRNDEVAACIRYRLREDVLAAVEATLEAREMLLDVAGDHTETVMPGFTHLQPAQPTTVAHYLHSYASALARDTERLLDAYGRINRSPLGAAAFAGTPFDIDRERTAELLGFDGVVRNSMDAASARDFLVETTAAAAGLATTLSGLAEDLVVFSKAGYVELDDAYASTSSIMPQKKNPDTMELVRATAGDTAAGLNALLTILKGLPRAYNRDLQRAHPHAFEALDAVTEATEVAAGAVATADWKEPALSEAAGEGFSTATGVADLLAMEGVPFRTAHELVARAAEAGGDYAALSAAAEDILGGPLSEHVDKAAVEAALDPESSVASRDSLGGPAPESMAAALSAAGQRLDADAEALRERRGALATAADERERVVSSYDSTAPE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMJ0</accession>
    <name>HIS3_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribosyl-AMP cyclohydrolase</fullName>
            <shortName evidence="1">PRA-CH</shortName>
            <ecNumber evidence="1">3.5.4.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisI</name>
        <name type="ordered locus">NP_5154A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the hydrolysis of the adenine ring of phosphoribosyl-AMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-(5-phospho-beta-D-ribosyl)-5'-AMP + H2O = 1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:20049"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:59457"/>
            <dbReference type="EC" id="3.5.4.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 3/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PRA-CH family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.19" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324278.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMJ0"/>
    <dbReference type="SMR" id="Q3IMJ0"/>
    <dbReference type="STRING" id="348780.NP_5154A"/>
    <dbReference type="EnsemblBacteria" id="CAI50668">
        <property type="protein sequence ID" value="CAI50668"/>
        <property type="gene ID" value="NP_5154A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702822"/>
    <dbReference type="KEGG" id="nph:NP_5154A"/>
    <dbReference type="eggNOG" id="arCOG02676">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048577_5_0_2"/>
    <dbReference type="OrthoDB" id="5853at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00008"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004635">
        <property type="term" value="F:phosphoribosyl-AMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004636">
        <property type="term" value="F:phosphoribosyl-ATP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.810:FF:000001">
        <property type="entry name" value="Histidine biosynthesis bifunctional protein HisIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.810">
        <property type="entry name" value="Phosphoribosyl-AMP cyclohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01021">
        <property type="entry name" value="HisI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026660">
        <property type="entry name" value="PRA-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002496">
        <property type="entry name" value="PRib_AMP_CycHydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038019">
        <property type="entry name" value="PRib_AMP_CycHydrolase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000768">
        <property type="entry name" value="PRK00051.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42945">
        <property type="entry name" value="HISTIDINE BIOSYNTHESIS BIFUNCTIONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42945:SF1">
        <property type="entry name" value="HISTIDINE BIOSYNTHESIS BIFUNCTIONAL PROTEIN HIS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01502">
        <property type="entry name" value="PRA-CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141734">
        <property type="entry name" value="HisI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000229847" description="Phosphoribosyl-AMP cyclohydrolase">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01021"/>
        </source>
    </evidence>
    <sequence length="120" mass="13357" checksum="046A3955DE5D8BF5" modified="2005-11-08" version="1">MSVDLAFDEQELLPAVAQDAESGDVLMLAYVSEEAVARSRETGYAHYYSRSREELWKKGGSSGHTQEIEEIRVDCDGDAILYLVEQAGGACHTGYDTCFHRTLDGDIVGEQVFDPEEIYE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMK8</accession>
    <name>END4_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable endonuclease 4</fullName>
            <ecNumber evidence="1">3.1.21.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Endodeoxyribonuclease IV</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Endonuclease IV</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nfo</name>
        <name type="ordered locus">NP_5118A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic (AP) sites, generating a 3'-hydroxyl group and a 5'-terminal sugar phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products.</text>
            <dbReference type="EC" id="3.1.21.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 3 Zn(2+) ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AP endonuclease 2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.2" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324260.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMK8"/>
    <dbReference type="SMR" id="Q3IMK8"/>
    <dbReference type="STRING" id="348780.NP_5118A"/>
    <dbReference type="EnsemblBacteria" id="CAI50650">
        <property type="protein sequence ID" value="CAI50650"/>
        <property type="gene ID" value="NP_5118A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702262"/>
    <dbReference type="KEGG" id="nph:NP_5118A"/>
    <dbReference type="eggNOG" id="arCOG01894">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025885_0_1_2"/>
    <dbReference type="OrthoDB" id="33250at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008833">
        <property type="term" value="F:deoxyribonuclease IV (phage-T4-induced) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00019">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.150:FF:000001">
        <property type="entry name" value="Probable endonuclease 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.150">
        <property type="entry name" value="Divalent-metal-dependent TIM barrel enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00152">
        <property type="entry name" value="Nfo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001719">
        <property type="entry name" value="AP_endonuc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018246">
        <property type="entry name" value="AP_endonuc_F2_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00587">
        <property type="entry name" value="nfo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21445:SF0">
        <property type="entry name" value="APURINIC-APYRIMIDINIC ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21445">
        <property type="entry name" value="ENDONUCLEASE IV ENDODEOXYRIBONUCLEASE IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00518">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="Xylose isomerase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00731">
        <property type="entry name" value="AP_NUCLEASE_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51432">
        <property type="entry name" value="AP_NUCLEASE_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_1000011325" description="Probable endonuclease 4">
        <location>
            <begin position="1"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00152"/>
        </source>
    </evidence>
    <sequence length="278" mass="30018" checksum="3815D20760EE9BD1" modified="2005-11-08" version="1">MVQVGAHTSIAGGVDNAVEEQLEYDGTCGQIFTHSPQVWQDPNIGDEEATAFRDRSAEAGVGPWVIHSSYLVNLCTPKDDLREKSIDSMQKEVDAAATLHIPYVNVHLGAHTGAGVEQGLDNAASALEELDIPDGVTVLIESDAGSGTKLGSTFEELAGVLDRCDQPLDVCLDTAHMFAAGYDLSTPEGVAETFEAFDETVGLDHLEYIHLNDSKHDCGTNKDEHAHIGEGKIGEAGMRAFINHEAVADVPFVLETPTENGKSYPWNIKRVRELYEEA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ING1</accession>
    <name>HEM1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamyl-tRNA reductase</fullName>
            <shortName evidence="1">GluTR</shortName>
            <ecNumber evidence="1">1.2.1.70</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hemA</name>
        <name type="ordered locus">NP_4502A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-4-amino-5-oxopentanoate + tRNA(Glu) + NADP(+) = L-glutamyl-tRNA(Glu) + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12344"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57501"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="EC" id="1.2.1.70"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">Possesses an unusual extended V-shaped dimeric structure with each monomer consisting of three distinct domains arranged along a curved 'spinal' alpha-helix. The N-terminal catalytic domain specifically recognizes the glutamate moiety of the substrate. The second domain is the NADPH-binding domain, and the third C-terminal domain is responsible for dimerization.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutamyl-tRNA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.70" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323957.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ING1"/>
    <dbReference type="SMR" id="Q3ING1"/>
    <dbReference type="STRING" id="348780.NP_4502A"/>
    <dbReference type="EnsemblBacteria" id="CAI50342">
        <property type="protein sequence ID" value="CAI50342"/>
        <property type="gene ID" value="NP_4502A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702804"/>
    <dbReference type="KEGG" id="nph:NP_4502A"/>
    <dbReference type="eggNOG" id="arCOG01036">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035113_0_1_2"/>
    <dbReference type="OrthoDB" id="4562at2157"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00316"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008883">
        <property type="term" value="F:glutamyl-tRNA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019353">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process from glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05213">
        <property type="entry name" value="NAD_bind_Glutamyl_tRNA_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.30:FF:000001">
        <property type="entry name" value="Glutamyl-tRNA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.30">
        <property type="entry name" value="Glutamyl-tRNA reductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00087">
        <property type="entry name" value="Glu_tRNA_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000343">
        <property type="entry name" value="4pyrrol_synth_GluRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015896">
        <property type="entry name" value="4pyrrol_synth_GluRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015895">
        <property type="entry name" value="4pyrrol_synth_GluRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018214">
        <property type="entry name" value="GluRdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036453">
        <property type="entry name" value="GluRdtase_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036343">
        <property type="entry name" value="GluRdtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006151">
        <property type="entry name" value="Shikm_DH/Glu-tRNA_Rdtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01035">
        <property type="entry name" value="hemA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43013">
        <property type="entry name" value="GLUTAMYL-TRNA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43013:SF1">
        <property type="entry name" value="GLUTAMYL-TRNA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00745">
        <property type="entry name" value="GlutR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05201">
        <property type="entry name" value="GlutR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01488">
        <property type="entry name" value="Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000445">
        <property type="entry name" value="4pyrrol_synth_GluRdtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69742">
        <property type="entry name" value="Glutamyl tRNA-reductase catalytic, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69075">
        <property type="entry name" value="Glutamyl tRNA-reductase dimerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00747">
        <property type="entry name" value="GLUTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000004654" description="Glutamyl-tRNA reductase">
        <location>
            <begin position="1"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="413"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="413"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="52"/>
            <end position="55"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="110"/>
            <end position="112"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="184"/>
            <end position="189"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for activity" evidence="1">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="453" mass="47809" checksum="839ADF48145910A7" modified="2005-11-08" version="1">MTVETGIVVGASVSHSHASIDQIETAADGSQQTAVEELLSKEGVSEAFVLQTCNRAEAYVVANDSETGTAALAGYLAGVDDDAIRMLSHNQSLKHLMAVACGLESLVIGEDQILGQVRDAYEDARGVGGVGPTLNDAVLKALHVGERARTETAINEGAVSLGSAAVRFAAERHGVTGSTALVVGAGEMATLAAKALDDPASRVIVANRTRERAERLAEELSTTASVVGVDSVDEAADAADVVVSATGSEDYVIDTADLGGVGETCVLDIAQPRDVAPGADGIDGVAVYDLDTIESVTDEARAKRREAAETVETMIDEEFEHLMTQYKRKRADQVIAAMYEGAERIKARELRTAVSKFEAEREDGLSEREHEIVESMADALVGQLLSAPTQSLRDAAEEDDWATINAALQLFGPGLEPEPTELPTVPDGPEGVPEELRERMSSGMLEQFSTNDD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-05-20" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IP34</accession>
    <name>THIE_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thiamine-phosphate synthase</fullName>
            <shortName evidence="1">TP synthase</shortName>
            <shortName evidence="1">TPS</shortName>
            <ecNumber evidence="1">2.5.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Thiamine-phosphate pyrophosphorylase</fullName>
            <shortName evidence="1">TMP pyrophosphorylase</shortName>
            <shortName evidence="1">TMP-PPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thiE</name>
        <name type="ordered locus">NP_4054A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = thiamine phosphate + CO2 + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:47844"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:62899"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = thiamine phosphate + CO2 + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:47848"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:62890"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4-methyl-5-(2-phosphooxyethyl)-thiazole + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + H(+) = thiamine phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22328"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:58296"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine phosphate from 4-amino-2-methyl-5-diphosphomethylpyrimidine and 4-methyl-5-(2-phosphoethyl)-thiazole: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiamine-phosphate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.3" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323734.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IP34"/>
    <dbReference type="SMR" id="Q3IP34"/>
    <dbReference type="STRING" id="348780.NP_4054A"/>
    <dbReference type="EnsemblBacteria" id="CAI50118">
        <property type="protein sequence ID" value="CAI50118"/>
        <property type="gene ID" value="NP_4054A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703246"/>
    <dbReference type="KEGG" id="nph:NP_4054A"/>
    <dbReference type="eggNOG" id="arCOG01089">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018272_3_2_2"/>
    <dbReference type="OrthoDB" id="85572at2157"/>
    <dbReference type="UniPathway" id="UPA00060">
        <property type="reaction ID" value="UER00141"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004789">
        <property type="term" value="F:thiamine-phosphate diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00564">
        <property type="entry name" value="TMP_TenI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000096">
        <property type="entry name" value="Thiamine-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00097">
        <property type="entry name" value="TMP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036206">
        <property type="entry name" value="ThiamineP_synth_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022998">
        <property type="entry name" value="ThiamineP_synth_TenI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034291">
        <property type="entry name" value="TMP_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00693">
        <property type="entry name" value="thiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20857">
        <property type="entry name" value="THIAMINE-PHOSPHATE PYROPHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20857:SF15">
        <property type="entry name" value="THIAMINE-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02581">
        <property type="entry name" value="TMP-TENI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51391">
        <property type="entry name" value="Thiamin phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000336439" description="Thiamine-phosphate synthase">
        <location>
            <begin position="1"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="37"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine</name>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine</name>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine</name>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="134"/>
            <end position="136"/>
        </location>
        <ligand>
            <name>2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:62899"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine</name>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:62899"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="187"/>
            <end position="188"/>
        </location>
        <ligand>
            <name>2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:62899"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00097"/>
        </source>
    </evidence>
    <sequence length="214" mass="22083" checksum="CA07FE2EB5590BA3" modified="2005-11-08" version="1">MTQDFGVYLVTGAEHSAGRSTAEVVEAAIRGGVDIVQLRDKTATARERYEVGTELRTLTRDAGVPLVVNDRLDLAAAIDADGVHLGDDDLPIEVAREQLGSDAIVGRSVSTPDAAREAEQAGADYLGVGAIYGTDSKDTDPEQSNIGLDRIRAVRDATSLPFVGIGGVTPDNAAPVVEAGADGVAVISAITAADDPEHATRRLADAVEGVAPNV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IQ21</accession>
    <name>GCS2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamate--cysteine ligase</fullName>
            <ecNumber evidence="1">6.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Gamma-glutamylcysteine synthetase</fullName>
            <shortName evidence="1">GCS</shortName>
            <shortName evidence="1">Gamma-GCS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gshA</name>
        <name type="ordered locus">NP_3372A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the synthesis of gamma-glutamylcysteine (gamma-GC), the main low-molecular-weight thiol compound instead of glutathione in halophilic archaea.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-cysteine + L-glutamate + ATP = gamma-L-glutamyl-L-cysteine + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13285"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutamate--cysteine ligase type 2 family. YbdK subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.2" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323397.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IQ21"/>
    <dbReference type="SMR" id="Q3IQ21"/>
    <dbReference type="STRING" id="348780.NP_3372A"/>
    <dbReference type="EnsemblBacteria" id="CAI49777">
        <property type="protein sequence ID" value="CAI49777"/>
        <property type="gene ID" value="NP_3372A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701754"/>
    <dbReference type="KEGG" id="nph:NP_3372A"/>
    <dbReference type="eggNOG" id="arCOG02722">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044848_1_0_2"/>
    <dbReference type="OrthoDB" id="287652at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004357">
        <property type="term" value="F:glutamate-cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042398">
        <property type="term" value="P:modified amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01609">
        <property type="entry name" value="Glu_cys_ligase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050141">
        <property type="entry name" value="GCL_type2/YbdK_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006336">
        <property type="entry name" value="GCS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011793">
        <property type="entry name" value="YbdK"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02050">
        <property type="entry name" value="gshA_cyan_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010045">
        <property type="entry name" value="PRK13518.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36510">
        <property type="entry name" value="GLUTAMATE--CYSTEINE LIGASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36510:SF1">
        <property type="entry name" value="GLUTAMATE--CYSTEINE LIGASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04107">
        <property type="entry name" value="GCS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000255819" description="Glutamate--cysteine ligase">
        <location>
            <begin position="1"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01609"/>
        </source>
    </evidence>
    <sequence length="362" mass="40568" checksum="84FFE129509D7FC8" modified="2005-11-08" version="1">MDLGSREAFDRTGTLGIEEEFFVVDEYGRPTAGTDELVYENEPPEPLKDRLDHELFKCVIETQTPTIGSLSAADDALAEVRKALVSHAETHGFGIAAAGLHPAAKWRELDHAEKPRYRAQLDRIQYPQHRNTTAGLHIHVGVDDADKAVWVANELRWYMPVMLALSANSPYWNGFDTGLQSARAKIFEALPNTGMPTAFEDFESFQSFERTMVETGSINDRGELWYDVRPHSEHGTVEVRTPDGQADPDHVLAFVEYTQALVEDLSARYEDGESGANHRRELLDENKWRALRHGHDAELLDRSLEESVPLGELVESECQRLGVSGIRDVYEAESGAEKQRRLLESEGMDALCGSLSVEWDSS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IT20</accession>
    <name>GSA_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamate-1-semialdehyde 2,1-aminomutase</fullName>
            <shortName evidence="1">GSA</shortName>
            <ecNumber evidence="1">5.4.3.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glutamate-1-semialdehyde aminotransferase</fullName>
            <shortName evidence="1">GSA-AT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hemL</name>
        <name type="ordered locus">NP_1246A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-4-amino-5-oxopentanoate = 5-aminolevulinate</text>
            <dbReference type="Rhea" id="RHEA:14265"/>
            <dbReference type="ChEBI" id="CHEBI:57501"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="5.4.3.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.3.8" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322350.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IT20"/>
    <dbReference type="SMR" id="Q3IT20"/>
    <dbReference type="STRING" id="348780.NP_1246A"/>
    <dbReference type="EnsemblBacteria" id="CAI48714">
        <property type="protein sequence ID" value="CAI48714"/>
        <property type="gene ID" value="NP_1246A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702810"/>
    <dbReference type="KEGG" id="nph:NP_1246A"/>
    <dbReference type="eggNOG" id="arCOG00918">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_1_5_2"/>
    <dbReference type="OrthoDB" id="6524at2157"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00317"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042286">
        <property type="term" value="F:glutamate-1-semialdehyde 2,1-aminomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000021">
        <property type="entry name" value="Glutamate-1-semialdehyde 2,1-aminomutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00375">
        <property type="entry name" value="HemL_aminotrans_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004639">
        <property type="entry name" value="4pyrrol_synth_GluAld_NH2Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000818">
        <property type="entry name" value="PRK00062.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43713">
        <property type="entry name" value="GLUTAMATE-1-SEMIALDEHYDE 2,1-AMINOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43713:SF3">
        <property type="entry name" value="GLUTAMATE-1-SEMIALDEHYDE 2,1-AMINOMUTASE 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000243649" description="Glutamate-1-semialdehyde 2,1-aminomutase">
        <location>
            <begin position="1"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00375"/>
        </source>
    </evidence>
    <sequence length="446" mass="48114" checksum="932DCB046BAC88A8" modified="2005-11-08" version="1">MNHEGSRSLYDRALSVLPGGVNSAVRAAPQPYPVFADHGDGGHVIDADGNRYIDWIQGLGPLLLGHDMPEQLQAAVQSRASEGPMYGMPSEIEVEHAEFVCRHVPSVEMIRFVNSGTEATVSACRLARAATGRNKIVVMQGGYHGAQESTLVEGDADDVAPSSAGIPQSFAEHTIPVPFNDPEAASEVFRAHGDDIAAVLVEPVQANMGIVYPEDGYHEALRSLCDDYGSLLIFDEVITGFRVGGLQCAQGKFDIDPDITTFGKIIGGGFPVGAIGGKTEYIEQFTPSGDVFQAGTFSGHPVTMAAGLETLKFCAEEDVYDHVNELGRQLREGLSDIVADQAPEYTVVGTDSLFKVVFTRGDGTPQGEACRNGCRQSSDCSRYGTCPTSATDVRDAETDRYARLFRPKMIEEGVLVSQNQFESNFVSYRHTAEDVEETLEAYKEAL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ITP9</accession>
    <name>RPIA_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribose-5-phosphate isomerase A</fullName>
            <ecNumber evidence="1">5.3.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Phosphoriboisomerase A</fullName>
            <shortName evidence="1">PRI</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpiA</name>
        <name type="ordered locus">NP_0786A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible conversion of ribose-5-phosphate to ribulose 5-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>aldehydo-D-ribose 5-phosphate = D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:14657"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="EC" id="5.3.1.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; pentose phosphate pathway; D-ribose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribose 5-phosphate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.6" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322120.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ITP9"/>
    <dbReference type="SMR" id="Q3ITP9"/>
    <dbReference type="STRING" id="348780.NP_0786A"/>
    <dbReference type="EnsemblBacteria" id="CAI48484">
        <property type="protein sequence ID" value="CAI48484"/>
        <property type="gene ID" value="NP_0786A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703117"/>
    <dbReference type="KEGG" id="nph:NP_0786A"/>
    <dbReference type="eggNOG" id="arCOG01122">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056590_1_0_2"/>
    <dbReference type="OrthoDB" id="19013at2157"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00412"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004751">
        <property type="term" value="F:ribose-5-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006014">
        <property type="term" value="P:D-ribose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009052">
        <property type="term" value="P:pentose-phosphate shunt, non-oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01398">
        <property type="entry name" value="RPI_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.260:FF:000018">
        <property type="entry name" value="Ribose-5-phosphate isomerase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1360:FF:000001">
        <property type="entry name" value="Ribose-5-phosphate isomerase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00170">
        <property type="entry name" value="Rib_5P_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020672">
        <property type="entry name" value="Ribose5P_isomerase_typA_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004788">
        <property type="entry name" value="Ribose5P_isomerase_type_A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001924">
        <property type="entry name" value="PRK00702.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00021">
        <property type="entry name" value="rpiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11934">
        <property type="entry name" value="RIBOSE-5-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11934:SF0">
        <property type="entry name" value="RIBOSE-5-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06026">
        <property type="entry name" value="Rib_5-P_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75445">
        <property type="entry name" value="D-ribose-5-phosphate isomerase (RpiA), lid domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000016951" description="Ribose-5-phosphate isomerase A">
        <location>
            <begin position="1"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="32"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="86"/>
            <end position="89"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="98"/>
            <end position="101"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="225" mass="23088" checksum="AA483E1BD71AED6B" modified="2005-11-08" version="1">MKQSGGTEAQKRRAGKQAADAVEDGMVVGLGTGSTAAASIRELGQRVEEGLDIRGIPTSYQSRQLAREADIPLTTLEEATPDVAIDGADQVAAGDLIKGGGAAHAREKLVDAAADRFLVVADETKLSPTLDIPVPVEVLPDAAPVVQRQVAALGGEPTLRAAERKDGPVVTDNGNLVIDCAFGEIAEPAALAEELSALPGAVEHGLFVGLADEILVGTDDGVDVR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-01-10" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ITQ5</accession>
    <name>AMZA_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Archaemetzincin</fullName>
            <ecNumber evidence="1">3.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">amzA</name>
        <name type="ordered locus">NP_0774A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probable zinc metalloprotease whose natural substrate is unknown.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) ions per subunit. One is catalytic, whereas the other seems to have a structural role.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M54 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322114.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ITQ5"/>
    <dbReference type="SMR" id="Q3ITQ5"/>
    <dbReference type="STRING" id="348780.NP_0774A"/>
    <dbReference type="EnsemblBacteria" id="CAI48478">
        <property type="protein sequence ID" value="CAI48478"/>
        <property type="gene ID" value="NP_0774A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700958"/>
    <dbReference type="KEGG" id="nph:NP_0774A"/>
    <dbReference type="eggNOG" id="arCOG00458">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108521_2_0_2"/>
    <dbReference type="OrthoDB" id="50281at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11375">
        <property type="entry name" value="Peptidase_M54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01842">
        <property type="entry name" value="Archaemetzincin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012962">
        <property type="entry name" value="Pept_M54_archaemetzincn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012091">
        <property type="entry name" value="Pept_M54_archaemetzncn_arc/bac"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033823">
        <property type="entry name" value="archmetzin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15910">
        <property type="entry name" value="ARCHAEMETZINCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15910:SF1">
        <property type="entry name" value="ARCHAEMETZINCIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07998">
        <property type="entry name" value="Peptidase_M54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005785">
        <property type="entry name" value="Zn-prot_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0482">Metalloprotease</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000159629" description="Archaemetzincin">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01842"/>
        </source>
    </evidence>
    <sequence length="173" mass="19183" checksum="90C0039A1E8B7BE7" modified="2005-11-08" version="1">MHVDIVPVGDLPAVVKREASSGLRSVYDCEVTIHDSQPVPDGAYDSGRDQYRAEEFIELASRVGNGEKNIAITDTDLYYRRRNYVFGLAYLSGKGSVISTHRLQTSSDGGFSEKSAGDIFADRVRKEVVHEVGHTLGLEHCDNSRCVMNFSPTVREVDVKEENLCGSCQRQIL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-07-10" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ITY5</accession>
    <name>Y614A_NATPD</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein NP_0614A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">NP_0614A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0440 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322034.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ITY5"/>
    <dbReference type="SMR" id="Q3ITY5"/>
    <dbReference type="STRING" id="348780.NP_0614A"/>
    <dbReference type="EnsemblBacteria" id="CAI48398">
        <property type="protein sequence ID" value="CAI48398"/>
        <property type="gene ID" value="NP_0614A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700905"/>
    <dbReference type="KEGG" id="nph:NP_0614A"/>
    <dbReference type="eggNOG" id="arCOG04665">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_190390_0_0_2"/>
    <dbReference type="OrthoDB" id="213887at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.100">
        <property type="entry name" value="MTH677-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024502">
        <property type="entry name" value="DUF3194"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035954">
        <property type="entry name" value="MTH677-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11419">
        <property type="entry name" value="DUF3194"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110783">
        <property type="entry name" value="Hypothetical protein MTH677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000293126" description="Uncharacterized protein NP_0614A">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="83" mass="8894" checksum="8CC7AD0E62FAEEFD" modified="2005-11-08" version="1">MTADDSDEPTADEIVEVASDAAEGLILSRYKQSEVTDMDVTVRFEDGTLDVDIYLNAPDDPDPGAVAQEAVEAAESAVDELFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ET84</accession>
    <name>A0A1U7ET84_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF192 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0004A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48093.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321732.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ET84"/>
    <dbReference type="STRING" id="348780.NP_0004A"/>
    <dbReference type="EnsemblBacteria" id="CAI48093">
        <property type="protein sequence ID" value="CAI48093"/>
        <property type="gene ID" value="NP_0004A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700736"/>
    <dbReference type="KEGG" id="nph:NP_0004A"/>
    <dbReference type="eggNOG" id="arCOG03116">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097039_1_1_2"/>
    <dbReference type="OrthoDB" id="6763at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1140">
        <property type="entry name" value="Protein of unknown function DUF192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003795">
        <property type="entry name" value="DUF192"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038695">
        <property type="entry name" value="Saro_0823-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37953">
        <property type="entry name" value="UPF0127 PROTEIN MJ1496"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37953:SF1">
        <property type="entry name" value="UPF0127 PROTEIN MJ1496"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02643">
        <property type="entry name" value="DUF192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48093.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="183" mass="19493" checksum="F994C4207BF8C372" modified="2017-05-10" version="1">MDTRRVVGYAVAAVALAAAAVLLAFSTGLIAPFLGDGYDPDAYDPDDSYGGDTDYEHTTVTVVDGETGAELGAVDAAIADTFNKRYLGLSATDGLPEDRGMFFVHDSPDDYTYVMRDMSFGIDIVYADADGTITAVHEAPAPGPNEDGDDHEYPGYGQYVLEVNKGWMADRGAGVGDKLRFEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ET92</accession>
    <name>A0A1U7ET92_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein-L-isoaspartate O-methyltransferase</fullName>
            <ecNumber evidence="9">2.1.1.77</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">L-isoaspartyl protein carboxyl methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Protein L-isoaspartyl methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Protein-beta-aspartate methyltransferase</fullName>
            <shortName evidence="9">PIMT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">pimT1</name>
        <name evidence="9" type="synonym">pcm</name>
        <name evidence="10" type="ordered locus">NP_0018A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 9">Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>[protein]-L-isoaspartate + S-adenosyl-L-methionine = [protein]-L-isoaspartate alpha-methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:12705"/>
            <dbReference type="Rhea" id="RHEA-COMP:12143"/>
            <dbReference type="Rhea" id="RHEA-COMP:12144"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90596"/>
            <dbReference type="ChEBI" id="CHEBI:90598"/>
            <dbReference type="EC" id="2.1.1.77"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.77" evidence="9"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321739.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ET92"/>
    <dbReference type="STRING" id="348780.NP_0018A"/>
    <dbReference type="EnsemblBacteria" id="CAI48100">
        <property type="protein sequence ID" value="CAI48100"/>
        <property type="gene ID" value="NP_0018A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703033"/>
    <dbReference type="KEGG" id="nph:NP_0018A"/>
    <dbReference type="eggNOG" id="arCOG00976">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055432_2_0_2"/>
    <dbReference type="OrthoDB" id="33618at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004719">
        <property type="term" value="F:protein-L-isoaspartate (D-aspartate) O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030091">
        <property type="term" value="P:protein repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000010">
        <property type="entry name" value="Protein-L-isoaspartate O-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00090">
        <property type="entry name" value="PIMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000682">
        <property type="entry name" value="PCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00080">
        <property type="entry name" value="pimt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001453">
        <property type="entry name" value="PRK00312.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579:SF0">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE(D-ASPARTATE) O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01135">
        <property type="entry name" value="PCMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 9 10" id="KW-0808">Transferase</keyword>
    <feature type="active site" evidence="9">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI48100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="212" mass="23025" checksum="1791BAFB5BF9B019" modified="2017-05-10" version="1">MSEESFAAQRDRMVDALAESGRIEREATLEALRAVPRHEFVPEPRREEAYADRPLPIGDGQTVSAPHMVGIMCDRLGLAAGDDVLEIGTGCGYHAAVTAEIVGDDNVYSVEYIERLAEAARERLDTLGYGGVSVRVGDGHEGWPEHAPYDAVYLTCATPAIPDPLVEQLRVGGRLLAPVGDTTQRLIEATKTDDGLERTQHGAVRFVRMQGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETC2</accession>
    <name>A0A1U7ETC2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Beta-lactamase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">NP_0092A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321776.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETC2"/>
    <dbReference type="STRING" id="348780.NP_0092A"/>
    <dbReference type="EnsemblBacteria" id="CAI48137">
        <property type="protein sequence ID" value="CAI48137"/>
        <property type="gene ID" value="NP_0092A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700760"/>
    <dbReference type="KEGG" id="nph:NP_0092A"/>
    <dbReference type="eggNOG" id="arCOG00504">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030571_5_1_2"/>
    <dbReference type="OrthoDB" id="197151at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06262">
        <property type="entry name" value="metallo-hydrolase-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051453">
        <property type="entry name" value="MBL_Glyoxalase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46233">
        <property type="entry name" value="HYDROXYACYLGLUTATHIONE HYDROLASE GLOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46233:SF3">
        <property type="entry name" value="HYDROXYACYLGLUTATHIONE HYDROLASE GLOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="5">
        <location>
            <begin position="14"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI48137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="203" mass="22586" checksum="E2375D57206CEC21" modified="2017-05-10" version="1">MDVYHVTANAETFTCNAYLVDGDRTVLVDAGAMDGVVDVVDEYTDMLDAVVLTHQHSDHIAELDAVLDAFDAPLYAYDDHPQRTNELDDGDQIFMGDEPFDVVYTPGHADDHVALVSETTLFSGDVVVHDDGAFDYGSFGRTDMPGQSRERLIESIQTLLDRMGEDVEHMYAGHGDTFHGDVRDVVETALQRAEKREPKYPEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETC5</accession>
    <name>A0A1U7ETC5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC-type transport system permease protein (Probable substrate macrolides)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">abc30p</name>
        <name evidence="10" type="ordered locus">NP_0102A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ABC-4 integral membrane protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321781.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETC5"/>
    <dbReference type="STRING" id="348780.NP_0102A"/>
    <dbReference type="EnsemblBacteria" id="CAI48142">
        <property type="protein sequence ID" value="CAI48142"/>
        <property type="gene ID" value="NP_0102A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702455"/>
    <dbReference type="KEGG" id="nph:NP_0102A"/>
    <dbReference type="eggNOG" id="arCOG02312">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_8_0_2"/>
    <dbReference type="OrthoDB" id="11469at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050250">
        <property type="entry name" value="Macrolide_Exporter_MacB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30572:SF4">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE YTRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30572">
        <property type="entry name" value="MEMBRANE COMPONENT OF TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="241"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="300"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="340"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="MacB-like periplasmic core" evidence="9">
        <location>
            <begin position="21"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="ABC3 transporter permease C-terminal" evidence="8">
        <location>
            <begin position="252"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI48142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="375" mass="38900" checksum="8F0631F2995EDE4D" modified="2017-05-10" version="1">MTRIPSLFVARRNLSRNRLRSALAALGILVGVVAIASLGIFGNVLAVGADDALGDIGTQVIITPNADAGISSIDDGTVTEIERVAGDAAVVPLRTDGGLVERGSATAAGTIYGIENPAAVYTADEGRLPERHRQGAVVGADLASTLELRVGDSIDVAGDSHRVIAVLEPVESFTPISPDDAVMLPEAAVGDGDGYEQVVVQTETGAAATAVADAVELTVNDRDERVDVFELSSVVDEIDEFFSLLSVFLIGLGGISLFVAGVSILNVMLMSTVERREEIGVMRAVGVPRRAVLRTMLFEAALLGLVGAAGGVVITALLVVGLYLATPVELWVVLDPTNGVYLAAAFAFGVLISVVSGLYPAWKAANERPVEALRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETD6</accession>
    <name>A0A1U7ETD6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">NP_0138A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321799.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETD6"/>
    <dbReference type="STRING" id="348780.NP_0138A"/>
    <dbReference type="EnsemblBacteria" id="CAI48160">
        <property type="protein sequence ID" value="CAI48160"/>
        <property type="gene ID" value="NP_0138A"/>
    </dbReference>
    <dbReference type="GeneID" id="32154992"/>
    <dbReference type="KEGG" id="nph:NP_0138A"/>
    <dbReference type="eggNOG" id="arCOG02589">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06408">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_0_2"/>
    <dbReference type="OrthoDB" id="3369at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009927">
        <property type="term" value="F:histidine phosphotransfer kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00156">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013656">
        <property type="entry name" value="PAS_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43047:SF72">
        <property type="entry name" value="OSMOSENSING HISTIDINE PROTEIN KINASE SLN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43047">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08448">
        <property type="entry name" value="PAS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Response regulatory" evidence="8">
        <location>
            <begin position="8"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="9">
        <location>
            <begin position="212"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="277"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="6">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI48160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="484" mass="53151" checksum="EBBF86AACFEA357B" modified="2017-05-10" version="1">MELRAELDVLLVEDNPGDARLIEKRLERADESLLASDHTLAHVDDLAGCLDALAESDWDVVLLDLGLPESNGLDTLSAVTDAADNIPVVVLTGLDDQQLALEAITEGAQDYLTKDDLNGSELSRSVRYAVERKKHEQKLKAERDFLDTVIESLPYPFYVVDVDDYTVTHANSRAPVEEGDTCHAATHGRDVPCDEADADLGCPLPSVVETGEPQELEHIHHTEDGEKRVYKVHAAPIFDDDGNVVQMAESNIDITDRVAYERRLEEQRDNLEILNEVVRHDIRNDLQLVTAYAEMLEAHVDDDGTQYLQTVKESAENAVDLTTTARDLAEVMLQPDVENRQVALDRSLERQLEEIRSTHSDAAVTVDGSLPTVDVVGNDMLGAVFRNLLKNAVQHNDKPLPEVSVSAVVDGDIAEIRVADNGPGVPDAQKEEIFGKGEKGLESEGTGVGLYLVRTLVESYGGEVWVEDNEPEGSVFIVELPVAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETE5</accession>
    <name>A0A1U7ETE5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histidine kinase</fullName>
            <ecNumber evidence="3">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">NP_0142A</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="3"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321801.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETE5"/>
    <dbReference type="STRING" id="348780.NP_0142A"/>
    <dbReference type="EnsemblBacteria" id="CAI48162">
        <property type="protein sequence ID" value="CAI48162"/>
        <property type="gene ID" value="NP_0142A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700769"/>
    <dbReference type="KEGG" id="nph:NP_0142A"/>
    <dbReference type="eggNOG" id="arCOG02358">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035392_0_0_2"/>
    <dbReference type="OrthoDB" id="106630at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000156">
        <property type="term" value="F:phosphorelay response regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007234">
        <property type="term" value="P:osmosensory signaling via phosphorelay pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12914">
        <property type="entry name" value="PDC1_DGC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000006">
        <property type="entry name" value="Sensor histidine kinase WalK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050351">
        <property type="entry name" value="BphY/WalK/GraS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033479">
        <property type="entry name" value="dCache_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42878:SF15">
        <property type="entry name" value="BACTERIOPHYTOCHROME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42878">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02743">
        <property type="entry name" value="dCache_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="247"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="14">
        <location>
            <begin position="269"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="13">
        <location>
            <begin position="343"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAI48162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="562" mass="60991" checksum="B3BDA3CB36993497" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETF4</accession>
    <name>A0A1U7ETF4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC-type transport system permease protein (Probable substrate branched-chain amino acids)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">livH5</name>
        <name evidence="10" type="synonym">abc09p1</name>
        <name evidence="10" type="ordered locus">NP_0172A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321816.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETF4"/>
    <dbReference type="STRING" id="348780.NP_0172A"/>
    <dbReference type="EnsemblBacteria" id="CAI48177">
        <property type="protein sequence ID" value="CAI48177"/>
        <property type="gene ID" value="NP_0172A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702394"/>
    <dbReference type="KEGG" id="nph:NP_0172A"/>
    <dbReference type="eggNOG" id="arCOG01270">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039929_2_1_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06582">
        <property type="entry name" value="TM_PBP1_LivH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052157">
        <property type="entry name" value="BCAA_transport_permease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11795:SF442">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11795">
        <property type="entry name" value="BRANCHED-CHAIN AMINO ACID TRANSPORT SYSTEM PERMEASE PROTEIN LIVH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="77"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="256"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI48177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="319" mass="33995" checksum="AD23ED3EF58C80B1" modified="2017-05-10" version="1">MLEAALFVSPELFVEQTLNGLFFGFILFMIATGLTIIFGVLGILNLAHGEFYALGAFLVFSIAGFFVGFVGDPTDPVSIALVAVLALVGVFVAAAILLPISAFLEAVFVRPIYDRDEVYQLLLTYALLLMLVDVLLLVWGGQPQRLEGVFNTVNQIPTTELVGFSYPSYNIFAILLGLCVFGFLVWFFERTKTGRIVRATAINREAATAMGVSTDRVFTLVFAMGGFFAGFAGAVHGAGPISADVGMGVNPLVLSFVVIVVGGLGSIKGAFVGALLIGVASRWATMIYPPAELAAPFAIMVAVLLLRPEGLYGTWGEIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETI0</accession>
    <name>A0A1U7ETI0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">HypE family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hypE2</name>
        <name evidence="4" type="ordered locus">NP_0244A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HypE family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321852.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETI0"/>
    <dbReference type="STRING" id="348780.NP_0244A"/>
    <dbReference type="EnsemblBacteria" id="CAI48213">
        <property type="protein sequence ID" value="CAI48213"/>
        <property type="gene ID" value="NP_0244A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702853"/>
    <dbReference type="KEGG" id="nph:NP_0244A"/>
    <dbReference type="eggNOG" id="arCOG00636">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041631_1_0_2"/>
    <dbReference type="OrthoDB" id="31494at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06061">
        <property type="entry name" value="PurM-like1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011854">
        <property type="entry name" value="HypE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30303:SF4">
        <property type="entry name" value="HYDROGENASE EXPRESSION_FORMATION PROTEIN HYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30303">
        <property type="entry name" value="HYDROGENASE ISOENZYMES FORMATION PROTEIN HYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005644">
        <property type="entry name" value="Hdrgns_mtr_HypE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PurM-like N-terminal" evidence="2">
        <location>
            <begin position="37"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="3">
        <location>
            <begin position="157"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="338" mass="34192" checksum="08A0F81B9EDDE8DE" modified="2017-05-10" version="1">MSDTSGKVDREFFETQIQPNLGAAADSTVLGPEHGIDFGVVTPAGEALVITADPLSVVPALGFERAGRLAVHSVLSDVAVSGVPPTHLSVTLTLPESMAEAEFAAFWGGVDETCSRLGVDIVTGHTGYHATDFPWVGGATAIGFGDPEQLVRPDGARPGDGLLLAGGPGREVAALFATLFSDTLDVTEETCQQAAARLEATTAVEDALAAADAAPVSAMHDATECGVKGALAELAAASGVHIDIESDATPSQPDIDAVCEAVGLDPWSVSARGTLLITGRPDALAAVADALEARGTDAAIVGAVTAGDGVHIDGEAVDHPGVDPAWETFRELGEAGSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETJ2</accession>
    <name>A0A1U7ETJ2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Small CPxCG-related zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0268A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321864.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETJ2"/>
    <dbReference type="STRING" id="348780.NP_0268A"/>
    <dbReference type="EnsemblBacteria" id="CAI48225">
        <property type="protein sequence ID" value="CAI48225"/>
        <property type="gene ID" value="NP_0268A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700808"/>
    <dbReference type="KEGG" id="nph:NP_0268A"/>
    <dbReference type="eggNOG" id="arCOG04757">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_152338_0_0_2"/>
    <dbReference type="OrthoDB" id="212944at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046243">
        <property type="entry name" value="DUF6276"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19792">
        <property type="entry name" value="DUF6276"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="130" mass="14118" checksum="3E3ED45C047C6657" modified="2017-05-10" version="1">MNCPSCDVPLVVFDVPVTYRDYIPEGAERAGLCPECLALAAAEDAAAESRFERITSSFPTDEDAAVPLAIAVGLLDSLALNRQALEELLTDVESAGVDPLLVLDRLHVQGGTNPEFDIERRRHQLTQLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETJ4</accession>
    <name>A0A1U7ETJ4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF411 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0250A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321855.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETJ4"/>
    <dbReference type="STRING" id="348780.NP_0250A"/>
    <dbReference type="EnsemblBacteria" id="CAI48216">
        <property type="protein sequence ID" value="CAI48216"/>
        <property type="gene ID" value="NP_0250A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700802"/>
    <dbReference type="KEGG" id="nph:NP_0250A"/>
    <dbReference type="eggNOG" id="arCOG03913">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112034_1_1_2"/>
    <dbReference type="OrthoDB" id="262137at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007332">
        <property type="entry name" value="DUF411"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04214">
        <property type="entry name" value="DUF411"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="172" mass="18308" checksum="C47C124A432B5871" modified="2017-05-10" version="1">MELSRRSFLATSAVATAGFAGCTGDSGDPDPPQQSTGTADWTWDGDVPVDSVVQYHQPSCGCCGEYVDYLERNGIAVSVEERPDFDSFVATKADLGVPESMRSCHTIEFGSYLVEGHVPLSAIERLFDDEAAVAGLAIPGMPQHAPGMGPPSDDPLTVYAFDDEGTRPFDDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETK9</accession>
    <name>A0A1U7ETK9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Peptidase M10 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0322A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321890.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETK9"/>
    <dbReference type="STRING" id="348780.NP_0322A"/>
    <dbReference type="EnsemblBacteria" id="CAI48252">
        <property type="protein sequence ID" value="CAI48252"/>
        <property type="gene ID" value="NP_0322A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700819"/>
    <dbReference type="KEGG" id="nph:NP_0322A"/>
    <dbReference type="eggNOG" id="arCOG04994">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_846260_0_0_2"/>
    <dbReference type="OrthoDB" id="9634at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="285"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="289"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="328" mass="35583" checksum="0E4DC46814A7C546" modified="2017-05-10" version="1">MDLSRRGVLAAGAVALAGCADLEEQADRRLSSSGGHPLSGTTSVAIYDRSESDHDLQALSTEAFDFWEANAPTYAGFETTFLFGADDLDLEVIFLNDGSELQGCADHATDDILGCAPLLQADHDPSFPVTIEVVVDDRPFGDVRVTLKHELGHALGLGHDDDPAYVMSNDIEDRLPEYEARIAVRDAFEEAWRTRNAATETYNEAVSRWDSGDPPAAESTFEAAADTYRDIAIHADEAETAARAFESMPRRDTVDREELSQYVDQLRAWADTAAEQAELMATAAAAADSGDRQTARDRQREAQDIGETLKSKPFPSPAAVAVAIGLMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETQ6</accession>
    <name>A0A1U7ETQ6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HTH domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0434A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321944.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETQ6"/>
    <dbReference type="STRING" id="348780.NP_0434A"/>
    <dbReference type="EnsemblBacteria" id="CAI48308">
        <property type="protein sequence ID" value="CAI48308"/>
        <property type="gene ID" value="NP_0434A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700855"/>
    <dbReference type="KEGG" id="nph:NP_0434A"/>
    <dbReference type="eggNOG" id="arCOG04688">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2327337_0_0_2"/>
    <dbReference type="OrthoDB" id="306709at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045490">
        <property type="entry name" value="DUF6432"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20024">
        <property type="entry name" value="DUF6432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="62"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="8"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="99" mass="11079" checksum="C6E985FD9F15E836" modified="2017-05-10" version="1">MQAKREYRDRAETEVAVLDALVDRAEEGMTVFELRSGTETDIDDLEAALSTLKEEGLITVENSDDEPMRIYPEPKVVPEPSENGEEPSLLSSLRDRLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETR9</accession>
    <name>A0A1U7ETR9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ArsR family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_0458A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321956.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETR9"/>
    <dbReference type="STRING" id="348780.NP_0458A"/>
    <dbReference type="EnsemblBacteria" id="CAI48320">
        <property type="protein sequence ID" value="CAI48320"/>
        <property type="gene ID" value="NP_0458A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700862"/>
    <dbReference type="KEGG" id="nph:NP_0458A"/>
    <dbReference type="eggNOG" id="arCOG01686">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068197_0_0_2"/>
    <dbReference type="OrthoDB" id="11368at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056525">
        <property type="entry name" value="HVO_1552_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12840">
        <property type="entry name" value="HTH_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24267">
        <property type="entry name" value="HVO_1552_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="HTH arsR-type" evidence="3">
        <location>
            <begin position="28"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="254" mass="26512" checksum="947E6411BEB13321" modified="2017-05-10" version="1">MSLLPSSPDVSPDGDPRVVGLDSEEADELMAALSSETARRLLAELHEDPAPPGELAERVDTSLQNTQYHLEKLEDAGAIEVVGTAYSEKGREMSVYGPADSPLVIFAGEEERASGLRAAVSRLFGGVLALAAGGAVVQQLFGEQLLAGTDEAAEPPEPTPTPEPEEPEEDDPGAFDVEPEPTPEATPEPTPEEVADTPAPAPEEAPDAADGLSAAIEGLFAAGLPPGLAFFLGGFVVLLAVVGTTYLRQRRTHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETS2</accession>
    <name>A0A1U7ETS2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF151 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0468A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321961.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETS2"/>
    <dbReference type="STRING" id="348780.NP_0468A"/>
    <dbReference type="EnsemblBacteria" id="CAI48325">
        <property type="protein sequence ID" value="CAI48325"/>
        <property type="gene ID" value="NP_0468A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700867"/>
    <dbReference type="KEGG" id="nph:NP_0468A"/>
    <dbReference type="eggNOG" id="arCOG01759">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096111_3_0_2"/>
    <dbReference type="OrthoDB" id="30741at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.690.10">
        <property type="entry name" value="Bifunctional nuclease domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036104">
        <property type="entry name" value="BFN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003729">
        <property type="entry name" value="Bi_nuclease_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15160:SF1">
        <property type="entry name" value="VON HIPPEL-LINDAU DISEASE TUMOR SUPPRESSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15160">
        <property type="entry name" value="VON HIPPEL-LINDAU PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02577">
        <property type="entry name" value="BFN_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103256">
        <property type="entry name" value="Hypothetical protein TM0160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51658">
        <property type="entry name" value="BFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BFN" evidence="1">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="148" mass="15736" checksum="ECE4B247D0C18366" modified="2017-05-10" version="1">MNAHIEGVRVAETPTGPVAIVTLGVDDADDVLPIFVGFEEAASIARGLDAADIGRPLTHDLLLDTIEALGARVTGVVVSGLDEGTYLADIHIETPRGEEVVDARPSDGLALAARTNADIDLNGKVFEAGREPKDEYEDLDDIREMMEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETU2</accession>
    <name>A0A1U7ETU2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF3426 domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0508A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321981.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETU2"/>
    <dbReference type="STRING" id="348780.NP_0508A"/>
    <dbReference type="EnsemblBacteria" id="CAI48345">
        <property type="protein sequence ID" value="CAI48345"/>
        <property type="gene ID" value="NP_0508A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700877"/>
    <dbReference type="KEGG" id="nph:NP_0508A"/>
    <dbReference type="eggNOG" id="arCOG07682">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1514657_0_0_2"/>
    <dbReference type="OrthoDB" id="350371at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047676">
        <property type="entry name" value="FxLYD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038353">
        <property type="entry name" value="FxLYD_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="178" mass="18871" checksum="CFC2F2C23D335CE1" modified="2017-05-10" version="1">MEIKDLDILAMALGAVAVIFSGFVLVYSVWGGLALFFGGVLAASIAFVRDRSVLAGRTVWVLFVLLAAAGVAGFIVGFGVFDSPDPRWEAEATLHDADTADATVIVTGTVGNHGDAPAERVTVTVTLYDADDETLATGTTELTQLAPRSTQQFYLRFDENPGELSAFADIEVELDVEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETU4</accession>
    <name>A0A1U7ETU4_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">UvrABC system protein A</fullName>
            <shortName evidence="14">UvrA protein</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Excinuclease ABC subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14 17" type="primary">uvrA</name>
        <name evidence="17" type="ordered locus">NP_0504A</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by UvrB, the UvrA molecules dissociate.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Forms a heterotetramer with UvrB during the search for lesions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the ABC transporter superfamily. UvrA family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321979.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETU4"/>
    <dbReference type="STRING" id="348780.NP_0504A"/>
    <dbReference type="EnsemblBacteria" id="CAI48343">
        <property type="protein sequence ID" value="CAI48343"/>
        <property type="gene ID" value="NP_0504A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703457"/>
    <dbReference type="KEGG" id="nph:NP_0504A"/>
    <dbReference type="eggNOG" id="arCOG04694">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001370_0_2_2"/>
    <dbReference type="OrthoDB" id="7896at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009380">
        <property type="term" value="C:excinuclease repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009381">
        <property type="term" value="F:excinuclease ABC activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03271">
        <property type="entry name" value="ABC_UvrA_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1580.10:FF:000002">
        <property type="entry name" value="UvrABC system protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.280">
        <property type="entry name" value="ABC transporter ATPase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1580.10">
        <property type="entry name" value="ABC transporter ATPase like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00205">
        <property type="entry name" value="UvrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004602">
        <property type="entry name" value="UvrA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041552">
        <property type="entry name" value="UvrA_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041102">
        <property type="entry name" value="UvrA_inter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001503">
        <property type="entry name" value="PRK00349.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00630">
        <property type="entry name" value="uvra"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43152">
        <property type="entry name" value="UVRABC SYSTEM PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43152:SF3">
        <property type="entry name" value="UVRABC SYSTEM PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17755">
        <property type="entry name" value="UvrA_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17760">
        <property type="entry name" value="UvrA_inter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 14" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 14" id="KW-0228">DNA excision</keyword>
    <keyword evidence="13 14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="12 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11 14" id="KW-0267">Excision nuclease</keyword>
    <keyword evidence="3 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0677">Repeat</keyword>
    <keyword evidence="14" id="KW-0742">SOS response</keyword>
    <keyword evidence="9 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ABC transporter" evidence="16">
        <location>
            <begin position="625"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="14">
        <location>
            <begin position="275"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="14">
        <location>
            <begin position="758"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="957"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="32"/>
            <end position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="659"/>
            <end position="666"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAI48343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="977" mass="107901" checksum="EEE0AD7844924858" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETV1</accession>
    <name>A0A1U7ETV1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA mismatch repair protein MutL</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 8" type="primary">mutL</name>
        <name evidence="8" type="ordered locus">NP_0536A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">This protein is involved in the repair of mismatches in DNA. It is required for dam-dependent methyl-directed DNA mismatch repair. May act as a 'molecular matchmaker', a protein that promotes the formation of a stable complex between two or more DNA-binding proteins in an ATP-dependent manner without itself being part of a final effector complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the DNA mismatch repair MutL/HexB family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321995.1">
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        <property type="gene ID" value="NP_0536A"/>
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    <dbReference type="KEGG" id="nph:NP_0536A"/>
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        <property type="taxonomic scope" value="Archaea"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="DNA mismatch repair protein MutL"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00058">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_1"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3 4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="210"/>
            <end position="331"/>
        </location>
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        <location>
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            <end position="660"/>
        </location>
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            <end position="510"/>
        </location>
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        <location>
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        </location>
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        <location>
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            <end position="429"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
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        <location>
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        <location>
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            <end position="504"/>
        </location>
    </feature>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
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        <source>
            <dbReference type="HAMAP-Rule" id="MF_00149"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00853"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM01340"/>
        </source>
    </evidence>
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            <dbReference type="EMBL" id="CAI48359.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="712" mass="75066" checksum="8BF0EBF32D282CC0" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETW7</accession>
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        <submittedName>
            <fullName evidence="3">DUF389 family protein</fullName>
        </submittedName>
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        <name evidence="3" type="ordered locus">NP_0578A</name>
    </gene>
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        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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    </dbReference>
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    <dbReference type="OrthoDB" id="3266at2157"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="111"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
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            <end position="163"/>
        </location>
    </feature>
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        <location>
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            <end position="200"/>
        </location>
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            <end position="238"/>
        </location>
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        <location>
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        </location>
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        <location>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="85"/>
            <end position="112"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI48380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="433" mass="45732" checksum="B9CCBC09F1573636" modified="2017-05-10" version="1">MRLVQVTIPAGKREAVLRVLDEEGIDYVVTDETSGREYTAVAYFPLPTSAVEPILEQLRDVGLEREAYTVVVSAETVVSKRFDDLKDSYAEKEESEERIARQEIEARAEELAASIPTYVVMTIVSAVIATAGLLLDSPATVVGSMVIAPLIGPAMTTAVGSVIDDAELFQRGVSLQVVGIVLAVAAATVFAVFVQVMNLVPPGLDPLSLAEVEERLSPNFLSLAVAIGAGIAGAVSLMTGISAALVGVMIAVALIPPAATVGIGIAYSDPALAVGSAVLVAVNMLSINLASLIVLWYAGYRPEHFFRRDKARIATLKRVAVLVVAIAVLSLFLGGVTYDSYQSAQTEQDIRNAIDTELEDPVYAGYTLVELEVETTAENLLFQRPTAATVTVGVPPDAGRPGLATGIETRVAAEAGVDIDIDVFYLERERGAG</sequence>
</entry>
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    <accession>A0A1U7ETX0</accession>
    <name>A0A1U7ETX0_NATPD</name>
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        <submittedName>
            <fullName evidence="1">DUF393 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0574A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="NP_0574A"/>
    </dbReference>
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    <dbReference type="KEGG" id="nph:NP_0574A"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2021565_0_0_2"/>
    <dbReference type="OrthoDB" id="195634at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007263">
        <property type="entry name" value="DCC1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04134">
        <property type="entry name" value="DCC1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="122" mass="14006" checksum="E31A86E5BCA02FEB" modified="2017-05-10" version="1">MSDATFVYDDDCGFCKWWADFFAQRTDLAMVGFDELSEAQLERLPDDYEDCSHLLTDAAVYSCGAAIEQALAEADIPPGSEEVFGFLRTFEDYERFRERAYREVADRRGLWGQFVSKDRVDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETZ7</accession>
    <name>A0A1U7ETZ7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transport protein (Probable substrate cationic amino acids)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">cat6</name>
        <name evidence="7" type="synonym">tp07</name>
        <name evidence="7" type="ordered locus">NP_0646A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322050.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETZ7"/>
    <dbReference type="STRING" id="348780.NP_0646A"/>
    <dbReference type="EnsemblBacteria" id="CAI48414">
        <property type="protein sequence ID" value="CAI48414"/>
        <property type="gene ID" value="NP_0646A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703284"/>
    <dbReference type="KEGG" id="nph:NP_0646A"/>
    <dbReference type="eggNOG" id="arCOG00009">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_15_8_2"/>
    <dbReference type="OrthoDB" id="43026at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050367">
        <property type="entry name" value="APC_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770">
        <property type="entry name" value="AMINO ACID TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770:SF11">
        <property type="entry name" value="INNER MEMBRANE TRANSPORT PROTEIN YBAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="81"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="132"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="196"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="270"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="336"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="361"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="417"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="791" mass="83105" checksum="8CB3D358F588BD12" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU18</accession>
    <name>A0A1U7EU18_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA primase large subunit PriL</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">priL</name>
        <name evidence="9" type="synonym">priB</name>
        <name evidence="9" type="ordered locus">NP_0686A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Regulatory subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. Stabilizes and modulates the activity of the small subunit, increasing the rate of DNA synthesis, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA repair.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="7">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterodimer of a small subunit (PriS) and a large subunit (PriL).</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the eukaryotic-type primase large subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322070.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU18"/>
    <dbReference type="STRING" id="348780.NP_0686A"/>
    <dbReference type="EnsemblBacteria" id="CAI48434">
        <property type="protein sequence ID" value="CAI48434"/>
        <property type="gene ID" value="NP_0686A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703051"/>
    <dbReference type="KEGG" id="nph:NP_0686A"/>
    <dbReference type="eggNOG" id="arCOG03013">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052778_0_0_2"/>
    <dbReference type="OrthoDB" id="46081at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06560">
        <property type="entry name" value="PriL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00701">
        <property type="entry name" value="DNA_primase_lrg_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058560">
        <property type="entry name" value="DNA_primase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023642">
        <property type="entry name" value="DNA_primase_lsu_PriL"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002590">
        <property type="entry name" value="PRK02249.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04104">
        <property type="entry name" value="DNA_primase_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26466">
        <property type="entry name" value="DNA_primase_lrg_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140914">
        <property type="entry name" value="PriB N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3 7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 7" id="KW-0408">Iron</keyword>
    <keyword evidence="6 7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="2 7" id="KW-0639">Primosome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA primase large subunit C-terminal" evidence="8">
        <location>
            <begin position="229"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="308"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI48434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="352" mass="38584" checksum="A24501ED9527F6EC" modified="2017-05-10" version="1">MERLHARYPFLDAARDAVEAADVDLAALVADGGPAVERGRERVERALLSGTVAAEEPRIWSDRAELLSYPVARVLVSLLDVPGAVEKYAAAEAATARERFVDDFEADLELKSSADQRLTLDRLLADFDMAGRVEPLGDGRFSVAATAYLELAAGLDDPQWRLATRPLSAGTLPVSRPELYTLLREAVARRVAEGLPLSVPDVIAEPLDGELAAIRRQIEDLDPPTGFETVTPGLFPPCMRALLSRARDGESLPDESAFSLVSFLLGVGLDRDAVIELCSFDGERAARVRDRIQRLETDDGVVATPPSCTVIDEYGDCVDPDETCNRIQHPLSYYERQLDQQPAEELTDWRAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU26</accession>
    <name>A0A1U7EU26_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Crossover junction endodeoxyribonuclease Hjc</fullName>
            <shortName evidence="11">Hjc</shortName>
            <ecNumber evidence="11">3.1.21.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Holliday junction resolvase Hjc</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 12" type="primary">hjc</name>
        <name evidence="12" type="ordered locus">NP_0680A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">A structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination. Cleaves 4-way DNA junctions introducing paired nicks in opposing strands, leaving a 5'-terminal phosphate and a 3'-terminal hydroxyl group that are subsequently ligated to produce recombinant products.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).</text>
            <dbReference type="EC" id="3.1.21.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the Holliday junction resolvase Hjc family.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.10" evidence="11"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322067.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU26"/>
    <dbReference type="STRING" id="348780.NP_0680A"/>
    <dbReference type="EnsemblBacteria" id="CAI48431">
        <property type="protein sequence ID" value="CAI48431"/>
        <property type="gene ID" value="NP_0680A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702826"/>
    <dbReference type="KEGG" id="nph:NP_0680A"/>
    <dbReference type="eggNOG" id="arCOG00919">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139546_0_0_2"/>
    <dbReference type="OrthoDB" id="34330at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00523">
        <property type="entry name" value="Holliday_junction_resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01490">
        <property type="entry name" value="HJ_Resolv_Hjc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002732">
        <property type="entry name" value="Hjc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014428">
        <property type="entry name" value="Hjc_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040854">
        <property type="entry name" value="Hol_resolv_Hjc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39651">
        <property type="entry name" value="HOLLIDAY JUNCTION RESOLVASE HJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39651:SF1">
        <property type="entry name" value="HOLLIDAY JUNCTION RESOLVASE HJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01870">
        <property type="entry name" value="Hjc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 11" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="9 11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 11" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5 11 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 11" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="11">
        <location>
            <position position="32"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="11">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAI48431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="172" mass="18719" checksum="0AD29DED668DFC9A" modified="2017-05-10" version="1">MANSNAKGDRRERELVTKLDEAGFAVMRAPASGSATDRELPDVLAGDGETFYAVEAKSSAGDPIYLSGEEIEALVYFARNFGAKPRVGVRFDREDWYFFHPGDLYETDGGNYRVKKETALADGTDFAEFTGQSEKVTLDEIAVGSDVDETTREVLGAVERGDLSADDAAEML</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUK3</accession>
    <name>A0A1U7EUK3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1138A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUK3"/>
    <dbReference type="STRING" id="348780.NP_1138A"/>
    <dbReference type="EnsemblBacteria" id="CAI48660">
        <property type="protein sequence ID" value="CAI48660"/>
        <property type="gene ID" value="NP_1138A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_1138A"/>
    <dbReference type="HOGENOM" id="CLU_2021569_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="122" mass="12978" checksum="DC0D6FB9733FB29A" modified="2017-05-10" version="1">MRQFFPGGDLSPLPEATTAQKRLSRAAFNLVSTGSTLNRESFRAVQAAAELPLATVDETTGADVDPAHEVVGSQFEAAEAFSNRTEDRVLTAMVSTSQLAAEYAAFVDSSVDEVIPGSRELN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUQ5</accession>
    <name>A0A1U7EUQ5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Alcohol dehydrogenase</fullName>
            <ecNumber evidence="6">1.1.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">adh</name>
        <name evidence="6" type="ordered locus">NP_1260A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.1" evidence="6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322357.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUQ5"/>
    <dbReference type="STRING" id="348780.NP_1260A"/>
    <dbReference type="EnsemblBacteria" id="CAI48721">
        <property type="protein sequence ID" value="CAI48721"/>
        <property type="gene ID" value="NP_1260A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702492"/>
    <dbReference type="KEGG" id="nph:NP_1260A"/>
    <dbReference type="eggNOG" id="arCOG01455">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_11_2_2"/>
    <dbReference type="OrthoDB" id="73567at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030554">
        <property type="term" value="F:adenyl nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043168">
        <property type="term" value="F:anion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051262">
        <property type="term" value="P:protein tetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08260">
        <property type="entry name" value="Zn_ADH6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050129">
        <property type="entry name" value="Zn_alcohol_dh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401:SF5">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="5">
        <location>
            <begin position="10"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI48721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="355" mass="37844" checksum="1C70DE405F5A7608" modified="2017-05-10" version="1">MRAVVFEEAGEPMEVQEVDRPEADADEIVVETEACGVCRSDWHAWQGDWDWIGVAPTPGLVFGHEPVGTVKEVGEDVSELSVGDRVCVPFNLSDGTCRYCKQGRANICERVVPMGFVPFQPGAFAEEFPVRNADHNAIKIPDGVDPVDVAGLGCRFATAFHGLVHRVDVTPGDWVAVHGCGGVGLSAIHVADALGANVIGVDLGEDKLDKAVELGADATVNVTEVEDVPQAVKAHTPSSRGVEVSVDALGVAETCRNSVNSLAKGGQNLQIGLTTSEEEGEVSLPVDNIVMEEREFVGSFGMPPHEYEEIFSMMAQDKIDPGKIVSETVSLEEVPEVIASMDDYGTVGIPVCDEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUT8</accession>
    <name>A0A1U7EUT8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1336A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48759.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322394.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUT8"/>
    <dbReference type="STRING" id="348780.NP_1336A"/>
    <dbReference type="EnsemblBacteria" id="CAI48759">
        <property type="protein sequence ID" value="CAI48759"/>
        <property type="gene ID" value="NP_1336A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701117"/>
    <dbReference type="KEGG" id="nph:NP_1336A"/>
    <dbReference type="eggNOG" id="arCOG10332">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1140592_0_0_2"/>
    <dbReference type="OrthoDB" id="197906at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48759.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="241" mass="27649" checksum="E27FE8B697AF11F3" modified="2017-05-10" version="1">MTENTPWKRLRGVAALSVQRAGAPLRQRVPFGLLRRGHRLLRRAAPRHWTSADPFSVVAVDPSRITHSILETAPKHPQWGRTVGGRWDVDTAPFADRPVAVAVRQHVDEGVPWAETELRTAFRRQLDRFGTAWGHTSMDGFETRCRTVRRLYESIRDDGYRRQEELPGGVPVLDEINVDIGRNGELLWRGYGQHRLAIAKTLHLDAVPVFVHRRHREWERKRRRGDVAATHPDAGHRSRCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUU4</accession>
    <name>A0A1U7EUU4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TRAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_1298A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322375.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUU4"/>
    <dbReference type="STRING" id="348780.NP_1298A"/>
    <dbReference type="EnsemblBacteria" id="CAI48740">
        <property type="protein sequence ID" value="CAI48740"/>
        <property type="gene ID" value="NP_1298A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701107"/>
    <dbReference type="KEGG" id="nph:NP_1298A"/>
    <dbReference type="eggNOG" id="arCOG01643">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1639934_0_0_2"/>
    <dbReference type="OrthoDB" id="199482at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TRAM" evidence="2">
        <location>
            <begin position="48"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="158" mass="17417" checksum="820F55865DF24D76" modified="2017-05-10" version="1">MPDCPLADDCPSFSERIEGMGCQHYGDRGGAEWCSHYSQPINDLKQQPVQVGEEVVVDVGDIHESGAGVGRTEDGFIVMVDGVLPEARAKVRITEVRSNHARADEVERLPLEEDEDGSSEPDTTDDSDPGAEEADDRSDRERALDREELGSRENFWGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUX9</accession>
    <name>A0A1U7EUX9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Biotin transport protein BioY</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">bioY</name>
        <name evidence="3" type="synonym">abc21u</name>
        <name evidence="3" type="ordered locus">NP_1394A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the BioY family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322423.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUX9"/>
    <dbReference type="STRING" id="348780.NP_1394A"/>
    <dbReference type="EnsemblBacteria" id="CAI48788">
        <property type="protein sequence ID" value="CAI48788"/>
        <property type="gene ID" value="NP_1394A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702431"/>
    <dbReference type="KEGG" id="nph:NP_1394A"/>
    <dbReference type="eggNOG" id="arCOG02986">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077931_0_0_2"/>
    <dbReference type="OrthoDB" id="50443at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015225">
        <property type="term" value="F:biotin transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1760.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003784">
        <property type="entry name" value="BioY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34295">
        <property type="entry name" value="BIOTIN TRANSPORTER BIOY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34295:SF1">
        <property type="entry name" value="BIOTIN TRANSPORTER BIOY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02632">
        <property type="entry name" value="BioY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016661">
        <property type="entry name" value="BioY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="1 2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="52"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="99"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="128"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="160"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR016661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="194" mass="19886" checksum="0CD415896BC11E6D" modified="2017-05-10" version="1">MTDTSTADVDLVGEEMALNVARTALFAALMGAFAYVSFPYPLSPAPVTLQVLGVFLAGLLLGPLWGGAAMVVYLLAGVLGAPVFSAGEAGIGVILGPQGGYLLSYPFAAAIVGGIVHRGTDIKPLETVSAALMIAAMILSTVVIYAFGIPFMWWNLEMTLLNAVIAGGVVFIPAEAAKMAAAIGIVRSDAIRAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV07</accession>
    <name>A0A1U7EV07_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable glycosyltransferase, type 2</fullName>
            <ecNumber evidence="2">2.4.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">gtl3</name>
        <name evidence="2" type="ordered locus">NP_1436A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.4.-.-" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322444.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV07"/>
    <dbReference type="STRING" id="348780.NP_1436A"/>
    <dbReference type="EnsemblBacteria" id="CAI48809">
        <property type="protein sequence ID" value="CAI48809"/>
        <property type="gene ID" value="NP_1436A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701150"/>
    <dbReference type="KEGG" id="nph:NP_1436A"/>
    <dbReference type="eggNOG" id="arCOG00895">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033536_9_0_2"/>
    <dbReference type="OrthoDB" id="11098at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859:SF91">
        <property type="entry name" value="DOLICHYL-PHOSPHATE BETA-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859">
        <property type="entry name" value="GLYCOSYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="1">
        <location>
            <begin position="44"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="255" mass="27344" checksum="3A99826A0DC905DF" modified="2017-05-10" version="1">MSSLGVVVPAFRPDVERLGTYLQDIADTLDPVVIRVELDDPDEATTAAVSDIGVSPVVVNTVDTRRGKGAAITDGFEALGTDRLAFADADGSTGAAELATVVAALEDADVAVGSRRHPESAVAGHQTLARRLLGDGFAWLARRLLAVGLYDYQCGAKALSAEAWSAVRDHIHEPGFAWDIELLAVAAAMELRIREVPIRWEDKPGSTVAPVRTTLSMLRALFVARHRAKRLQNSGVHRLLGVGEDPPALIDREER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV26</accession>
    <name>A0A1U7EV26_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Molybdopterin-binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_1534A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV26"/>
    <dbReference type="STRING" id="348780.NP_1534A"/>
    <dbReference type="EnsemblBacteria" id="CAI48858">
        <property type="protein sequence ID" value="CAI48858"/>
        <property type="gene ID" value="NP_1534A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701173"/>
    <dbReference type="KEGG" id="nph:NP_1534A"/>
    <dbReference type="eggNOG" id="arCOG00264">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130239_0_0_2"/>
    <dbReference type="OrthoDB" id="271771at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="entry name" value="Oxidoreductase, molybdopterin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000572">
        <property type="entry name" value="OxRdtase_Mopterin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036374">
        <property type="entry name" value="OxRdtase_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00174">
        <property type="entry name" value="Oxidored_molyb"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="42"/>
            <end position="124"/>
        </location>
    </feature>
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        <location>
            <begin position="122"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="144" mass="15454" checksum="5DE222784DC9C3B4" modified="2017-05-10" version="1">MSDQFDTLDRSHVAGDAGGDLRALAERHGLDTLDRETACSSGEPIEGRWRGVPLSALLDAADPEATHLVVEAADGFRVCVPVADALFGILAVERLDAPDEGLPRLVVPSVDGPRMAKHVRRLDTERLPPGTEPETLERLAPDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV47</accession>
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        <recommendedName>
            <fullName evidence="2">DUF7527 domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="3" type="ordered locus">NP_1526A</name>
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    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
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            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
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                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="AlphaFoldDB" id="A0A1U7EV47"/>
    <dbReference type="STRING" id="348780.NP_1526A"/>
    <dbReference type="EnsemblBacteria" id="CAI48854">
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        <property type="gene ID" value="NP_1526A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701170"/>
    <dbReference type="KEGG" id="nph:NP_1526A"/>
    <dbReference type="eggNOG" id="arCOG03730">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021291_0_0_2"/>
    <dbReference type="OrthoDB" id="157503at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055949">
        <property type="entry name" value="DUF7527"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33472:SF28">
        <property type="entry name" value="BROMO AND FHA DOMAIN-CONTAINING PROTEIN DDB_G0267958"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33472">
        <property type="entry name" value="OS01G0106600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24371">
        <property type="entry name" value="DUF7527"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7527" evidence="2">
        <location>
            <begin position="519"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="490"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="219"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="278"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="299"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="349"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="421"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="438"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="490"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="751" mass="80763" checksum="1F011A346579935F" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV51</accession>
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        <submittedName>
            <fullName evidence="2">DUF4341/DUF395 family protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">NP_1572A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
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            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48877.1"/>
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    <dbReference type="EnsemblBacteria" id="CAI48877">
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    </dbReference>
    <dbReference type="GeneID" id="3701189"/>
    <dbReference type="KEGG" id="nph:NP_1572A"/>
    <dbReference type="eggNOG" id="arCOG04788">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037802_2_1_2"/>
    <dbReference type="OrthoDB" id="200254at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="entry name" value="DUF6691"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20398">
        <property type="entry name" value="DUF6691"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="153" mass="15889" checksum="3DCB59D8E74AB6C1" modified="2017-05-10" version="1">MSDDRHPLFMPLVVVGGLLFGFGLGYSQMARPEIVLSFLELYDLGLLFVMGGAIAVAAPVFLLASRSGKSAPLTGAAYTRRLKRLDRNVLVGGVVFGIGWGLSGICPGAAYASLGIGNYPMLWGVAGMFIGAYGQGALRSMLASGDAGSAIRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV85</accession>
    <name>A0A1U7EV85_NATPD</name>
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        <submittedName>
            <fullName evidence="2">DUF583 domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1670A</name>
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    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
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            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
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    <dbReference type="EnsemblBacteria" id="CAI48926">
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        <property type="gene ID" value="NP_1670A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701217"/>
    <dbReference type="KEGG" id="nph:NP_1670A"/>
    <dbReference type="eggNOG" id="arCOG02471">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040510_0_0_2"/>
    <dbReference type="OrthoDB" id="105377at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
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    </dbReference>
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        <property type="entry name" value="BacA/B"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="270"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="285"/>
        </location>
    </feature>
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        <location>
            <begin position="296"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="328"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="381"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="391"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="418"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="432" mass="45516" checksum="77B27F9A0FDD2AFF" modified="2017-05-10" version="1">MFGPDPLDELAIPDGTHVREHDLVTDGDVLVGGQSVVELGVRGSNIVAGERVQFGGDIEADGDCRLDMWCEVDGNVLVGEDAYLGERVHITGQLIVSGDLDIGDDVDIEEGFEANGWIVIRNPMPTIVFLFVYLSHLLRMGDEEAAEEVAEEFLTDDQAADPVVIPRGGRVSDDAWRVSTPATIGDDCRLHGNIRATDIEVGTATEVFGSLRAKGDIRIGDDTVVHGDVTTKDGDVAVAPGVRIRGDISCEACTLSEAADVEGTIRARDGIEFERERSPSGERGRGAVASGHETTETEAEPEDSPDEPAAKPATDEQATDADRGGAAADDDPGTGSESPEESTPDAAADGSEQPEADDESHERSAAFVPLDTTEPKPAVRSLEEARHDWTDGQGDTTGDGAADTEADDHSDPTTNGVGDDDVSAVVTDTEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVB8</accession>
    <name>A0A1U7EVB8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Probable iron-sulfur protein (4Fe-4S)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">NP_1722A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322585.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVB8"/>
    <dbReference type="STRING" id="348780.NP_1722A"/>
    <dbReference type="EnsemblBacteria" id="CAI48952">
        <property type="protein sequence ID" value="CAI48952"/>
        <property type="gene ID" value="NP_1722A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701237"/>
    <dbReference type="KEGG" id="nph:NP_1722A"/>
    <dbReference type="eggNOG" id="arCOG00336">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023081_5_0_2"/>
    <dbReference type="OrthoDB" id="230142at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006089">
        <property type="term" value="P:lactate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024185">
        <property type="entry name" value="FTHF_cligase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003741">
        <property type="entry name" value="LUD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004452">
        <property type="entry name" value="LutB/LldF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47153">
        <property type="entry name" value="LACTATE UTILIZATION PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47153:SF2">
        <property type="entry name" value="LACTATE UTILIZATION PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02589">
        <property type="entry name" value="LUD_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="361"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI48952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="725" mass="78394" checksum="7A150C0E85DF57CD" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVD0</accession>
    <name>A0A1U7EVD0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Acyl-CoA dehydrogenase</fullName>
            <ecNumber evidence="11">1.3.8.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">acd11</name>
        <name evidence="11" type="ordered locus">NP_1754A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.-" evidence="11"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322601.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVD0"/>
    <dbReference type="STRING" id="348780.NP_1754A"/>
    <dbReference type="EnsemblBacteria" id="CAI48968">
        <property type="protein sequence ID" value="CAI48968"/>
        <property type="gene ID" value="NP_1754A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702475"/>
    <dbReference type="KEGG" id="nph:NP_1754A"/>
    <dbReference type="eggNOG" id="arCOG01708">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_1_0_2"/>
    <dbReference type="OrthoDB" id="275197at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000002">
        <property type="entry name" value="Acyl-CoA dehydrogenase fadE12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050741">
        <property type="entry name" value="Acyl-CoA_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083:SF13">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083">
        <property type="entry name" value="MEDIUM-CHAIN SPECIFIC ACYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016578">
        <property type="entry name" value="HsaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0274">FAD</keyword>
    <keyword evidence="4 7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6 7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="10">
        <location>
            <begin position="8"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="9">
        <location>
            <begin position="129"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="8">
        <location>
            <begin position="240"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI48968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="405" mass="44661" checksum="2E9B892F19F5BC7A" modified="2017-05-10" version="1">MLSYNDSEKATELAERANDLMEEVVLPKEREMAGGMAVSDGTLEELREAARDYGVYAPQIDEEYGGMGYDFRDVLPTFEEAGRSLLGAAAMRVDAPDEGNMHLLELHGSELQKEEYLEPLVAGDIVSGFSMTEPMQGGGSDPKMLKTTAEKDGDEWVINGHKWWTTKGIQADILLVFARTDQEAHPYEGCSVFIVPADADGVEIERNIPHLGSETHGIGHAEIKYNNVRVPEEHLLGQEGMGFAHVQERLGPARLTHCMRYSGMAERALDIATAYMSEREAFGSKLAEKQVPRHEVAEHRTRLAAARALIRQAADSISAGNEARLEVSMAKVFTANVTQEAVDTSLQFCGGNGIAKDLPIADFYESVRQFRLVDGADEVHKRVISREAFSDVKSEELEPVTRFDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVE9</accession>
    <name>A0A1U7EVE9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Carbon starvation protein CstA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cstA</name>
        <name evidence="8" type="ordered locus">NP_1810A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322628.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVE9"/>
    <dbReference type="STRING" id="348780.NP_1810A"/>
    <dbReference type="EnsemblBacteria" id="CAI48996">
        <property type="protein sequence ID" value="CAI48996"/>
        <property type="gene ID" value="NP_1810A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702641"/>
    <dbReference type="KEGG" id="nph:NP_1810A"/>
    <dbReference type="eggNOG" id="arCOG04286">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010531_4_1_2"/>
    <dbReference type="OrthoDB" id="77715at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="P:cellular response to starvation"/>
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</entry>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
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                <person name="Palm P."/>
                <person name="Rodewald K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </evidence>
    <sequence length="338" mass="36993" checksum="9C9233D35166EB5A" modified="2017-05-10" version="1">MSSELPADVAPEFFATMVESVGVGVGIYGADGRYVYVNEAYASLFGASTAELIDTPLWDVVDDIEADRFESYWNSFEEGETRTAESVHSYGGEGVPVGTVTTRRTIDGTAYHFGTIKDISERKAREKELRRQNERLENFASIVSHDLRNPLNVAQGYIDLLREDVDRDELRLVDNALDRMDVLIRELLELAQGGDQVGEAEPVALCAAAEDAWETVSTPRATLHPPSSTVTVLADQTRLQQLFENLFRNAVEHGGQDVVVTVGVTDEGFYVADDGVGIPADRREQVFETGYTTSEQGTGFGLGIVQQIASGHGWEVRLSESVDGGARFDVVGVSFTDR</sequence>
</entry>
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        </lineage>
    </organism>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
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            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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        </subcellularLocation>
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        <location>
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            <end position="152"/>
        </location>
    </feature>
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            <end position="138"/>
        </location>
    </feature>
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        <location>
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            <end position="152"/>
        </location>
    </feature>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="152" mass="16393" checksum="68E507AD87BCCB19" modified="2017-05-10" version="1">MDRRTKAVVGGVGIAVLLAILATTTMGAAAEFVTPTDLEEDDDYQNELVKLEGQVTDLEEGPPIAFAVDDGNYTKAVTYDGEMPETMSEGRIVVAEGHFDGETLVADDLTVRAHEGEHPDDHPGGVDADAHDESHFEGDSDYDNGDYDKDDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVN2</accession>
    <name>A0A1U7EVN2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type transport system ATP-binding protein (Probable substrate urea/short-chain amides)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">urtE</name>
        <name evidence="7" type="synonym">abc10a1</name>
        <name evidence="7" type="ordered locus">NP_1996A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322718.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVN2"/>
    <dbReference type="STRING" id="348780.NP_1996A"/>
    <dbReference type="EnsemblBacteria" id="CAI49089">
        <property type="protein sequence ID" value="CAI49089"/>
        <property type="gene ID" value="NP_1996A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702397"/>
    <dbReference type="KEGG" id="nph:NP_1996A"/>
    <dbReference type="eggNOG" id="arCOG00924">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OrthoDB" id="97750at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015807">
        <property type="term" value="P:L-amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03224">
        <property type="entry name" value="ABC_TM1139_LivF_branched"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052156">
        <property type="entry name" value="BCAA_Transport_ATP-bd_LivF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43820:SF5">
        <property type="entry name" value="HIGH-AFFINITY BRANCHED-CHAIN AMINO ACID TRANSPORT ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43820">
        <property type="entry name" value="HIGH-AFFINITY BRANCHED-CHAIN AMINO ACID TRANSPORT ATP-BINDING PROTEIN LIVF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="3"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="232" mass="24945" checksum="2922F22D7D388166" modified="2017-05-10" version="1">MSLTVTGLTAGYDGTPILRDVDCRVDGGEVVGIVGKNGVGKTTLLKTIMGLLDPDSGTVEYRGETIDALSADERAGLGIGYVPQGRDVFPGLSVRENLLIGENIGNETEALYDRVYEYFPILEERASQDAATMSGGQQQMLAIGRALVGNPDLLLVDEPSEGVQPSIVQSITEDLARINEEFGTTILFVEQNLSVIQGLADRCYAMDKGRLVDELDAEAIQDRGRLESHLVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVN4</accession>
    <name>A0A1U7EVN4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_1958A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVN4"/>
    <dbReference type="STRING" id="348780.NP_1958A"/>
    <dbReference type="EnsemblBacteria" id="CAI49070">
        <property type="protein sequence ID" value="CAI49070"/>
        <property type="gene ID" value="NP_1958A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_1958A"/>
    <dbReference type="HOGENOM" id="CLU_2784213_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="68" mass="7687" checksum="1CBF993498827F5B" modified="2017-05-10" version="1">MFDELVGATGWLDCAQSTIGRALDRPKFCAIWSTVVFRSSHELRQKQGFICAPARTHSYAVSLKRQSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVR1</accession>
    <name>A0A1U7EVR1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cupin 2 barrel domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2116A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322777.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVR1"/>
    <dbReference type="STRING" id="348780.NP_2116A"/>
    <dbReference type="EnsemblBacteria" id="CAI49149">
        <property type="protein sequence ID" value="CAI49149"/>
        <property type="gene ID" value="NP_2116A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701354"/>
    <dbReference type="KEGG" id="nph:NP_2116A"/>
    <dbReference type="eggNOG" id="arCOG03006">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144575_1_0_2"/>
    <dbReference type="OrthoDB" id="305577at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02208">
        <property type="entry name" value="cupin_RmlC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051610">
        <property type="entry name" value="GPI/OXD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35848">
        <property type="entry name" value="OXALATE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35848:SF9">
        <property type="entry name" value="SLL1358 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin type-2" evidence="2">
        <location>
            <begin position="38"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="118" mass="12626" checksum="51F16B54C06A322B" modified="2017-05-10" version="1">MDFSVATTDDIDSVVPDEYGGMWFFREPLDCAALGISLLELEPEGRGKPHDHADDGQEEVYLVVDGELTIALGGGDDEPPAEERTLTTGEAMRVGPGTWRALSNRGDEPVRVVVAGAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVT8</accession>
    <name>A0A1U7EVT8_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Taxis protein CheF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cheF1</name>
        <name evidence="2" type="ordered locus">NP_2166A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in taxis signal transduction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with chemotaxis (Che) proteins as well as flagella accessory (Fla) proteins.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322802.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVT8"/>
    <dbReference type="STRING" id="348780.NP_2166A"/>
    <dbReference type="EnsemblBacteria" id="CAI49174">
        <property type="protein sequence ID" value="CAI49174"/>
        <property type="gene ID" value="NP_2166A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701369"/>
    <dbReference type="KEGG" id="nph:NP_2166A"/>
    <dbReference type="eggNOG" id="arCOG02394">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_968406_0_0_2"/>
    <dbReference type="OrthoDB" id="227825at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007381">
        <property type="entry name" value="CheF1/F2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42201">
        <property type="entry name" value="TAXIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42201:SF1">
        <property type="entry name" value="TAXIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04283">
        <property type="entry name" value="CheF-arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF026802">
        <property type="entry name" value="UCP026802"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR026802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="287" mass="32362" checksum="77B9607BF6CFD904" modified="2017-05-10" version="1">MSKSERKLLDTTGRFTQVVRDGTKLNDISWTDGRILLSNRRLVLAGTDGKQTIKLTNVDKIEGRYDVNRTIAQVTDYVSIRVGTDVFLVSTKESESFERTLHKTILDGEIVLIKHPAIEGGVVQDVSWRKARIKVDEDAVNLAVEDGSFVQIEIDDVGTVERNERQVKSKERPVLEAEHTEDETSVETYLSGNRKHCSVVESVLKRGAEQNASSIDLSKDEEEVLMALYSGVSPFEVPEFLDMDPDAVEEIYERLIDLDVLQEVRIRREVALKPRGRNIASESMNSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVX4</accession>
    <name>A0A1U7EVX4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Acetolactate synthase small subunit</fullName>
            <ecNumber evidence="8">2.2.1.6</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ilvN</name>
        <name evidence="8" type="ordered locus">NP_2200A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetolactate synthase small subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.6" evidence="8"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
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    <dbReference type="STRING" id="348780.NP_2200A"/>
    <dbReference type="EnsemblBacteria" id="CAI49191">
        <property type="protein sequence ID" value="CAI49191"/>
        <property type="gene ID" value="NP_2200A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702871"/>
    <dbReference type="KEGG" id="nph:NP_2200A"/>
    <dbReference type="eggNOG" id="arCOG04445">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055003_1_0_2"/>
    <dbReference type="OrthoDB" id="85792at2157"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00055"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00059"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990610">
        <property type="term" value="F:acetolactate synthase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04878">
        <property type="entry name" value="ACT_AHAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.260:FF:000001">
        <property type="entry name" value="Acetolactate synthase, small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1150">
        <property type="entry name" value="ACT-like. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004789">
        <property type="entry name" value="Acetalactate_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027271">
        <property type="entry name" value="Acetolactate_synth/TF_NikR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019455">
        <property type="entry name" value="Acetolactate_synth_ssu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039557">
        <property type="entry name" value="AHAS_ACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054480">
        <property type="entry name" value="AHAS_small-like_ACT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00119">
        <property type="entry name" value="acolac_sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008864">
        <property type="entry name" value="PRK11895.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30239">
        <property type="entry name" value="ACETOLACTATE SYNTHASE SMALL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30239:SF0">
        <property type="entry name" value="ACETOLACTATE SYNTHASE SMALL SUBUNIT 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22629">
        <property type="entry name" value="ACT_AHAS_ss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10369">
        <property type="entry name" value="ALS_ss_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACT" evidence="7">
        <location>
            <begin position="48"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="210" mass="23564" checksum="2C1F1622FFA9FE28" modified="2017-05-10" version="1">MSRRQDERRNGLEGPEPSERQRPVGRRNEQGIRIDPEVAAEHEPRRTIISAYVKNEPGVLARVSGLFHRRQFNIESLTVGPTQNEGYSRITLVVEEPDPGIDQIKKQLQKVLPVVHVRELDNDPVARELVILKVDGDEPDKVQAITEMYDGETLDAGPQTITVQITGDEGKIDDAIDAYEQFDIREIARTGQAALARGAEETARVDEKHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVY7</accession>
    <name>A0A1U7EVY7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TIGR03663 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2284A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322860.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EVY7"/>
    <dbReference type="STRING" id="348780.NP_2284A"/>
    <dbReference type="EnsemblBacteria" id="CAI49233">
        <property type="protein sequence ID" value="CAI49233"/>
        <property type="gene ID" value="NP_2284A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701400"/>
    <dbReference type="KEGG" id="nph:NP_2284A"/>
    <dbReference type="eggNOG" id="arCOG00562">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_488026_0_0_2"/>
    <dbReference type="OrthoDB" id="313515at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019962">
        <property type="entry name" value="CHP03663"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016950">
        <property type="entry name" value="Manno-Trfase_MA4085_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038731">
        <property type="entry name" value="RgtA/B/C-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03663">
        <property type="entry name" value="flippase activity-associated protein Agl23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41710:SF2">
        <property type="entry name" value="GLYCOSYL TRANSFERASE FAMILY 39_83 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41710">
        <property type="entry name" value="GLYCOSYL TRANSFERASE, FAMILY 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13231">
        <property type="entry name" value="PMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF030218">
        <property type="entry name" value="Mannosyltr_MA4085_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="317"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="348"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="373"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="408"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase RgtA/B/C/D-like" evidence="2">
        <location>
            <begin position="58"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="595" mass="64911" checksum="92C4FF864E10CEC5" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW18</accession>
    <name>A0A1U7EW18_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Homolog to virus structural protein HRPV2-VP5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2342A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW18"/>
    <dbReference type="STRING" id="348780.NP_2342A"/>
    <dbReference type="EnsemblBacteria" id="CAI49262">
        <property type="protein sequence ID" value="CAI49262"/>
        <property type="gene ID" value="NP_2342A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_2342A"/>
    <dbReference type="eggNOG" id="arCOG07782">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_445247_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058677">
        <property type="entry name" value="ORF4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26255">
        <property type="entry name" value="Viral_env_HRPV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Envelope protein N-terminal" evidence="2">
        <location>
            <begin position="93"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="601" mass="65383" checksum="FCAB023F5C3355EB" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EW32</accession>
    <name>A0A1U7EW32_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">XerC/D-like integrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">NP_2370A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EW32"/>
    <dbReference type="STRING" id="348780.NP_2370A"/>
    <dbReference type="EnsemblBacteria" id="CAI49276">
        <property type="protein sequence ID" value="CAI49276"/>
        <property type="gene ID" value="NP_2370A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_2370A"/>
    <dbReference type="eggNOG" id="arCOG01250">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045500_0_0_2"/>
    <dbReference type="OrthoDB" id="198497at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00397">
        <property type="entry name" value="DNA_BRE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core-binding (CB)" evidence="6">
        <location>
            <begin position="13"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="5">
        <location>
            <begin position="120"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="320"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="320"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="352"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="362" mass="41735" checksum="8910925A56190D77" modified="2017-05-10" version="1">MSRNRSREAPSEWSPRNAAERYIKHRASDTTESSRSGWWYRLKLFVEWCEEVGLETVSDIQPLDIDEYHDIRAEAVAPVTLEGEMATLQEYLRYLEGLDAVADDLSEAVHVPNLDASQRSNDVKLSTPEAMAMLQYFRETPAVRASRKHVFLELVWFTGARQSGLRALDLRDVHLDDAFVWFKHRPSEGTGLKNNLDGERPVSLPSGVVDVLREYIHENRNSETDVHGRAPLFTTLQGRPSGDSVRKWCYLATLPCLHSDCPHGKDRESCDWTGYKYASKCPSTRSPHRIRTGSITYQLNIGFPTEVVANRVNASPKTIRDHYDKADRQERRRRQRRRMESDRRGYVQQMDFDYENDIGSDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWI0</accession>
    <name>A0A1U7EWI0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Thioesterase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2700A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323066.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWI0"/>
    <dbReference type="STRING" id="348780.NP_2700A"/>
    <dbReference type="EnsemblBacteria" id="CAI49441">
        <property type="protein sequence ID" value="CAI49441"/>
        <property type="gene ID" value="NP_2700A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702490"/>
    <dbReference type="KEGG" id="nph:NP_2700A"/>
    <dbReference type="eggNOG" id="arCOG00773">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050164_3_2_2"/>
    <dbReference type="OrthoDB" id="15030at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052816">
        <property type="term" value="F:long-chain fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03442">
        <property type="entry name" value="BFIT_BACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040170">
        <property type="entry name" value="Cytosol_ACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033120">
        <property type="entry name" value="HOTDOG_ACOT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11049">
        <property type="entry name" value="ACYL COENZYME A THIOESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11049:SF24">
        <property type="entry name" value="CYTOSOLIC ACYL COENZYME A THIOESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51770">
        <property type="entry name" value="HOTDOG_ACOT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HotDog ACOT-type" evidence="2">
        <location>
            <begin position="5"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="159" mass="17270" checksum="5FD8647DB568C056" modified="2017-05-10" version="1">MPTLADSKTEMTELLMPNGTNNLGRALGGTVLHWMDICAAVSSMRFAGTQCVTASMDHVDFVSPIDLGEVAVVEAYAYDTGETSIEVKVNVSAEDPRCDERRPAASSFFTFVAVDENGRPTETPELDCPTAAEEQLRDDAIAERRDRLQEVADRLGGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWK7</accession>
    <name>A0A1U7EWK7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha/beta hydrolase fold protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_2714A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323073.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWK7"/>
    <dbReference type="STRING" id="348780.NP_2714A"/>
    <dbReference type="ESTHER" id="natpd-q3iqz6">
        <property type="family name" value="6_AlphaBeta_hydrolase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI49448">
        <property type="protein sequence ID" value="CAI49448"/>
        <property type="gene ID" value="NP_2714A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701533"/>
    <dbReference type="KEGG" id="nph:NP_2714A"/>
    <dbReference type="eggNOG" id="arCOG01653">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_13_4_2"/>
    <dbReference type="OrthoDB" id="194600at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46438">
        <property type="entry name" value="ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46438:SF2">
        <property type="entry name" value="ALPHA_BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="62"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="307" mass="33170" checksum="5C08EB70DF1B23D3" modified="2017-05-10" version="1">MAPKHLVRNAAVGLGAVVVGNRLLRCSPETLAPPLARTQQTYEWQHRDVAYTEAGDPDAPDLVLLHAPELPRSSHEFRYVIDALSETHHVFAPDLPGFGRSAQLPETYDAEGYSGFLQSFLSDMTEQPTVVAASVTAAYAVAAGTDANAATDIDEYVLICPTTTAPQTTRRLAPAYSLPVAGEAFRNVTMSKPMLRYRFGTDGFAEPSAMTAEWLRYSWQASHRQGGHRLLAARAAGRLDSDIDLGSVLSETATTTTLLWGREADSPPVAAGRRLATAADARLVVFENAATLPHAEHPEPFVELFDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWP1</accession>
    <name>A0A1U7EWP1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DHH/RecJ family phosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2874A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
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    </organism>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
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    </dbReference>
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            <end position="102"/>
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            <end position="198"/>
        </location>
    </feature>
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        <location>
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            <end position="136"/>
        </location>
    </feature>
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        <location>
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            <end position="163"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
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            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50126"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI49528.1"/>
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    </evidence>
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        <source>
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        </source>
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</entry>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <name>FAD</name>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="Pfam" id="PF18158"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI49558.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
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    </evidence>
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</entry>
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    <accession>A0A1U7EWT6</accession>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="project" value="UniProtKB-KW"/>
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    <feature type="transmembrane region" description="Helical" evidence="1">
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    <feature type="transmembrane region" description="Helical" evidence="1">
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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        </location>
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            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="340"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="380"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="412"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="468"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="524"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome oxidase subunit I profile" evidence="2">
        <location>
            <begin position="4"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="581" mass="63913" checksum="B440F75E5F86C5EF" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWV2</accession>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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            <authorList>
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
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            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="GeneID" id="3701615"/>
    <dbReference type="KEGG" id="nph:NP_2984A"/>
    <dbReference type="eggNOG" id="arCOG03951">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="329477at2157"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Aur1/Ipt1"/>
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        <property type="entry name" value="IPC_Synthase-Related"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31310:SF7">
        <property type="entry name" value="PA-PHOSPHATASE RELATED-FAMILY PROTEIN DDB_G0268928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14378">
        <property type="entry name" value="PAP2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
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            <end position="26"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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    </feature>
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        </location>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
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            <dbReference type="EMBL" id="CAI49583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="297" mass="33090" checksum="06A40EFD88AFA0DB" modified="2017-05-10" version="1">MLFPFVIGRLAAVVLLLLLAGTVLFVGRERLALTRRELRTRIAAVAPYAGLLAVVLVANKVARDVGPDVSLLIGVGITGQLERIDRQILWPLFREETPQFVVWLQSMATQELTAYFSFIYVYGYVFLLVFPLVAYFALPRPEPLRRTIVAYCANYVIGVVCYVLFIAYGPRNIIVEQADGLLYSQYAQYQFVTTAVNDETNVFPSLHTSMAVTAALLAWTTRDEYPLWVPVAALLAVSVAISTMYLGIHWATDVVFGIALAWVSVELGKRYEETPPTLGRLKAGAGRVWRAIPVSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWW8</accession>
    <name>A0A1U7EWW8_NATPD</name>
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        <submittedName>
            <fullName evidence="4">NUDIX family hydrolase</fullName>
        </submittedName>
    </protein>
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        <name evidence="4" type="ordered locus">NP_3054A</name>
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    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <lineage>
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            <taxon>Natronomonas</taxon>
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    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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            <authorList>
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                <person name="Palm P."/>
                <person name="Rodewald K."/>
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            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
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        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
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        <property type="gene ID" value="NP_3054A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702988"/>
    <dbReference type="KEGG" id="nph:NP_3054A"/>
    <dbReference type="eggNOG" id="arCOG01073">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062658_5_0_2"/>
    <dbReference type="OrthoDB" id="104705at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006753">
        <property type="term" value="P:nucleoside phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019693">
        <property type="term" value="P:ribose phosphate metabolic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03424">
        <property type="entry name" value="NUDIX_ADPRase_Nudt5_UGPPase_Nudt14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="UDP/ADP-SUGAR PYROPHOSPHATASE"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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            <end position="178"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI49618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="183" mass="20192" checksum="A9BE9DEC994DDC84" modified="2017-05-10" version="1">MDKDALAWETLASERDYTCPGFDVIRDDVRLPNGTETEFHYVSEPPSVVILPFRPDGDVVVIEEWRQAVGRVSYGLPAGGLETDDEDVIAAAHRELAEETGYEADAIEQLGTYEPANGLFDSVFHYVVAHGCRPTAEQQLDDNESITVETASFDDLRDRAAVGDLRDGRTALGVLQYAVRKDS</sequence>
</entry>
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    <accession>A0A1U7EWY6</accession>
    <name>A0A1U7EWY6_NATPD</name>
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        <submittedName>
            <fullName evidence="1">HTH domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3068A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWY6"/>
    <dbReference type="STRING" id="348780.NP_3068A"/>
    <dbReference type="EnsemblBacteria" id="CAI49625">
        <property type="protein sequence ID" value="CAI49625"/>
        <property type="gene ID" value="NP_3068A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701644"/>
    <dbReference type="KEGG" id="nph:NP_3068A"/>
    <dbReference type="eggNOG" id="arCOG01345">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_152992_0_0_2"/>
    <dbReference type="OrthoDB" id="229881at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="97" mass="11192" checksum="882BBAB11FBC17B2" modified="2017-05-10" version="1">MIDRLARQLRKEERDLLVLEIILQQHPIGIERIAEEAGLDEHKTRHSMRMLEDDDLIEPTPQGAVPTDGVEERIGRIHGGIDSLIDRMEELKQYGNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWY9</accession>
    <name>A0A1U7EWY9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HTH domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3058A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323242.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWY9"/>
    <dbReference type="STRING" id="348780.NP_3058A"/>
    <dbReference type="EnsemblBacteria" id="CAI49620">
        <property type="protein sequence ID" value="CAI49620"/>
        <property type="gene ID" value="NP_3058A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701640"/>
    <dbReference type="KEGG" id="nph:NP_3058A"/>
    <dbReference type="eggNOG" id="arCOG03067">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124803_1_0_2"/>
    <dbReference type="OrthoDB" id="10985at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12840">
        <property type="entry name" value="HTH_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="124" mass="14189" checksum="1A8E6C71D28D4C6D" modified="2017-05-10" version="1">MVRDPLADDDAPDTEAVLEALHDDDCRAILAELDEPRTARELLDRCDIPRSTLYRKVDLLSEATLIREGTEISDDGSHASRYHVDFEEVIITREESSLNAEIERPARRADERLAELWSEVQKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX04</accession>
    <name>A0A1U7EX04_NATPD</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3060A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323243.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX04"/>
    <dbReference type="STRING" id="348780.NP_3060A"/>
    <dbReference type="DNASU" id="3701641"/>
    <dbReference type="EnsemblBacteria" id="CAI49621">
        <property type="protein sequence ID" value="CAI49621"/>
        <property type="gene ID" value="NP_3060A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701641"/>
    <dbReference type="KEGG" id="nph:NP_3060A"/>
    <dbReference type="eggNOG" id="arCOG03915">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159082_2_1_2"/>
    <dbReference type="OrthoDB" id="170398at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055943">
        <property type="entry name" value="DUF7521"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24365">
        <property type="entry name" value="DUF7521"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="96" mass="10075" checksum="A1BE98F897A3CF3A" modified="2017-05-10" version="1">MVHASVDIEVQFAIAVVKTLILVLGGIVTYLAYSAYQRTGDETLFQLSVGLALVGVGVLLGGFTFELLDVSLGVGVLIESLFVLAGLSVIAYSLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX19</accession>
    <name>A0A1U7EX19_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Probable S-adenosylmethionine-dependent methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_3110A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323268.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX19"/>
    <dbReference type="STRING" id="348780.NP_3110A"/>
    <dbReference type="EnsemblBacteria" id="CAI49646">
        <property type="protein sequence ID" value="CAI49646"/>
        <property type="gene ID" value="NP_3110A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701656"/>
    <dbReference type="KEGG" id="nph:NP_3110A"/>
    <dbReference type="eggNOG" id="arCOG04583">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029501_2_1_2"/>
    <dbReference type="OrthoDB" id="18536at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051422">
        <property type="entry name" value="AlkB_tRNA_MeTrf/Diox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13069">
        <property type="entry name" value="ALKYLATED DNA REPAIR PROTEIN ALKB HOMOLOG 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13069:SF21">
        <property type="entry name" value="ALKYLATED DNA REPAIR PROTEIN ALKB HOMOLOG 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="3">
        <location>
            <begin position="42"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="210" mass="22825" checksum="EEB135FFB716D218" modified="2017-05-10" version="1">MDDRPGVRATYERIADHFAETREYPWPEVESFCADRHVVTALDVGCGNGRHTELLGAHADHVIGIDASGRLLALARERAPNAAFLEADAAALPLADGVVDLAVYVATLHHLPSRELRRQSLDELARVLDESGTALVSAWSTAHDRFDADEDAEDGFDTDVEWTLPGGETVPRFYHIYAPAEFDTDIAESDLTAVDSFVSSGNCYAVVEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX38</accession>
    <name>A0A1U7EX38_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Histidine triad family protein (Homolog to bis(5'-nucleosyl)-tetraphosphatase)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hit1</name>
        <name evidence="4" type="ordered locus">NP_3194A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323310.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX38"/>
    <dbReference type="STRING" id="348780.NP_3194A"/>
    <dbReference type="EnsemblBacteria" id="CAI49688">
        <property type="protein sequence ID" value="CAI49688"/>
        <property type="gene ID" value="NP_3194A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702824"/>
    <dbReference type="KEGG" id="nph:NP_3194A"/>
    <dbReference type="eggNOG" id="arCOG00419">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056776_1_2_2"/>
    <dbReference type="OrthoDB" id="210764at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01275">
        <property type="entry name" value="FHIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052908">
        <property type="entry name" value="AP-4-A_phosphorylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039383">
        <property type="entry name" value="FHIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42997:SF1">
        <property type="entry name" value="AP-4-A PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42997">
        <property type="entry name" value="HIT FAMILY HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="3">
        <location>
            <begin position="26"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="2">
        <location>
            <begin position="121"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="179" mass="19952" checksum="3E027382D17D3E15" modified="2017-05-10" version="1">MDQVFAPWRIKWVEREDKNEAIDGCPFCVLPERDDASESLVVAESEHAFVLLNNYPYNPGHAMVIPRPHVGEYPELEPDVLFDHARLKQRTIEALNAAFEPDGINAGLNLGEASGGSIDDHLHTHLVPRWHGDTNFMPVCSDTKVIVQAVEETHEQLMEAFAAQPDTEPLDSGAVFVDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX43</accession>
    <name>A0A1U7EX43_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transducer protein Htr31</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">htr31</name>
        <name evidence="9" type="ordered locus">NP_3212A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323319.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX43"/>
    <dbReference type="STRING" id="348780.NP_3212A"/>
    <dbReference type="EnsemblBacteria" id="CAI49697">
        <property type="protein sequence ID" value="CAI49697"/>
        <property type="gene ID" value="NP_3212A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702846"/>
    <dbReference type="KEGG" id="nph:NP_3212A"/>
    <dbReference type="eggNOG" id="arCOG02318">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_116_0_2"/>
    <dbReference type="OrthoDB" id="116658at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11386">
        <property type="entry name" value="MCP_signal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.950">
        <property type="entry name" value="Methyl-accepting chemotaxis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004090">
        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32089:SF112">
        <property type="entry name" value="LYSOZYME-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32089">
        <property type="entry name" value="METHYL-ACCEPTING CHEMOTAXIS PROTEIN MCPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00260">
        <property type="entry name" value="CHEMTRNSDUCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58104">
        <property type="entry name" value="Methyl-accepting chemotaxis protein (MCP) signaling domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="PAS" evidence="7">
        <location>
            <begin position="192"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="8">
        <location>
            <begin position="347"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="6">
        <location>
            <begin position="403"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="13"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="642"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="418"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="477"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="678"/>
            <end position="695"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI49697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="695" mass="74965" checksum="6B3DC3C5470641C5" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX63</accession>
    <name>A0A1U7EX63_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Halocyanin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">hcp6</name>
        <name evidence="8" type="ordered locus">NP_3232A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323329.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX63"/>
    <dbReference type="STRING" id="348780.NP_3232A"/>
    <dbReference type="EnsemblBacteria" id="CAI49707">
        <property type="protein sequence ID" value="CAI49707"/>
        <property type="gene ID" value="NP_3232A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701693"/>
    <dbReference type="KEGG" id="nph:NP_3232A"/>
    <dbReference type="eggNOG" id="arCOG02917">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084115_1_3_2"/>
    <dbReference type="OrthoDB" id="4392at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000923">
        <property type="entry name" value="BlueCu_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34192">
        <property type="entry name" value="PLASTOCYANIN MAJOR ISOFORM, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34192:SF10">
        <property type="entry name" value="PLASTOCYANIN MAJOR ISOFORM, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00127">
        <property type="entry name" value="Copper-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0186">Copper</keyword>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Blue (type 1) copper" evidence="7">
        <location>
            <begin position="39"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="139" mass="14761" checksum="BF85C62FEC189D95" modified="2017-05-10" version="1">MDRRAFLATTGAAAAASVAGCLGRGLSDEDFDIGMSANAFLPEEYEIDVGETVVWGNNGSRGHTVTAYENSIPEDATYFASGGYDDEGTARDDWHNTAGGNIPPGETFAHTFNTPGDHHYVCIPHEPGGMIGVIRVREP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX88</accession>
    <name>A0A1U7EX88_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Small CPxCG-related zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3348A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323385.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX88"/>
    <dbReference type="EnsemblBacteria" id="CAI49765">
        <property type="protein sequence ID" value="CAI49765"/>
        <property type="gene ID" value="NP_3348A"/>
    </dbReference>
    <dbReference type="GeneID" id="77383466"/>
    <dbReference type="KEGG" id="nph:NP_3348A"/>
    <dbReference type="eggNOG" id="arCOG07584">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_211277_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049701">
        <property type="entry name" value="HVO_2523-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041914">
        <property type="entry name" value="HVO_2523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="40" mass="4527" checksum="4A84BC703B127525" modified="2017-05-10" version="1">MSDDSGRPCPMCGTQMYKRHCKYVCPQHGVVVDCSDPFTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXA4</accession>
    <name>A0A1U7EXA4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acetyl-CoA C-acyltransferase</fullName>
            <ecNumber evidence="4">2.3.1.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">acaB3</name>
        <name evidence="4" type="ordered locus">NP_3420A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.16" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323421.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXA4"/>
    <dbReference type="STRING" id="348780.NP_3420A"/>
    <dbReference type="EnsemblBacteria" id="CAI49801">
        <property type="protein sequence ID" value="CAI49801"/>
        <property type="gene ID" value="NP_3420A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702461"/>
    <dbReference type="KEGG" id="nph:NP_3420A"/>
    <dbReference type="eggNOG" id="arCOG01278">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035425_4_0_2"/>
    <dbReference type="OrthoDB" id="167534at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003988">
        <property type="term" value="F:acetyl-CoA C-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00829">
        <property type="entry name" value="SCP-x_thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055140">
        <property type="entry name" value="Thiolase_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870">
        <property type="entry name" value="ACETYL-COA C-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870:SF6">
        <property type="entry name" value="ACETYL-COA C-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22691">
        <property type="entry name" value="Thiolase_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="1" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="2">
        <location>
            <begin position="15"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="3">
        <location>
            <begin position="245"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="389" mass="39879" checksum="E3A9B0D189E71DB5" modified="2017-05-10" version="1">MPQPVLAAVGSSPVGQTDLPGRDLFSLAIAEAFDGLPDPASLADAVYVGNQSETYEHQIMQGTLLAEWAGLRNVPAERVEGCAAAGALALRHAVKDVRNGEHEGVLACGVEKMTAGGTGGATDALSAAFDRAIEQRSGVTAPSQYALLAQRYLHETDATEEDLARIAVKNHRNAAANPRAQFSEPIELEAAIESPPVAPPLKLYDCAPVGDGAAVVLVTTEERAAELDAPSVAIGGTGAAANNIAVAERDMTDIAGARIAAETAYEEAGLTPDDVDIAEVHDAFTVSEALLAEAVGFAPKGTGYQSYRPPSERADGWTDVSLSTSGGLKARGHPIGATGLLQAIEAYEQLTGQAPNRGPPAPETALLLNEGGVADAVTVAHVLTAGGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXB7</accession>
    <name>A0A1U7EXB7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">SpoVR family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_3450A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323436.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXB7"/>
    <dbReference type="STRING" id="348780.NP_3450A"/>
    <dbReference type="EnsemblBacteria" id="CAI49816">
        <property type="protein sequence ID" value="CAI49816"/>
        <property type="gene ID" value="NP_3450A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701776"/>
    <dbReference type="KEGG" id="nph:NP_3450A"/>
    <dbReference type="eggNOG" id="arCOG04632">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010179_1_0_2"/>
    <dbReference type="OrthoDB" id="193903at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007390">
        <property type="entry name" value="Spore_V_R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057008">
        <property type="entry name" value="SpoVR-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056174">
        <property type="entry name" value="SpoVR_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30029">
        <property type="entry name" value="STAGE V SPORULATION PROTEIN R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30029:SF2">
        <property type="entry name" value="STAGE V SPORULATION PROTEIN R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04293">
        <property type="entry name" value="SpoVR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24755">
        <property type="entry name" value="SpoVR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVR protein-like N-terminal" evidence="2">
        <location>
            <begin position="15"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="SpoVR protein-like N-terminal" evidence="2">
        <location>
            <begin position="471"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="domain" description="SpoVR-like C-terminal" evidence="3">
        <location>
            <begin position="547"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="639"/>
            <end position="661"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="661" mass="76504" checksum="45C6E2ED6217FCBD" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXH7</accession>
    <name>A0A1U7EXH7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Probable oxidoreductase (Short-chain dehydrogenase family)</fullName>
            <ecNumber evidence="4">1.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_3552A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323487.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXH7"/>
    <dbReference type="STRING" id="348780.NP_3552A"/>
    <dbReference type="EnsemblBacteria" id="CAI49867">
        <property type="protein sequence ID" value="CAI49867"/>
        <property type="gene ID" value="NP_3552A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701804"/>
    <dbReference type="KEGG" id="nph:NP_3552A"/>
    <dbReference type="eggNOG" id="arCOG01265">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_10_2"/>
    <dbReference type="OrthoDB" id="161871at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44196">
        <property type="entry name" value="DEHYDROGENASE/REDUCTASE SDR FAMILY MEMBER 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44196:SF1">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE SDR FAMILY MEMBER 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketoreductase" evidence="3">
        <location>
            <begin position="17"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="257" mass="26611" checksum="06BDD5839809F442" modified="2017-05-10" version="1">MGSSPNVGVTESSDTNPVALITGASSGIGAATARHFAHSGTNVVLAARNETSLTAVADDCEAAGSNALAVPTDVTEYDAVAALVDATIDRFGRLDTVVVNAGIGEKRDVPMSELPLEQFERVTETNVHGAYYTTKAALPALSDANGSLVFVGSYKGKHPSTSTPVYAASKWWLRGFALSVAGRVGPDGVGVTLVNPSGVTTGFGSEFRERTNDAALDDASTLDPSDVAAAIAYAVGQEAPATVTELDLNRRDIYERF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXQ9</accession>
    <name>A0A1U7EXQ9_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-(apurinic or apyrimidinic site) lyase</fullName>
            <ecNumber evidence="2">4.2.99.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">NP_3754A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the type-1 OGG1 family.</text>
    </comment>
    <dbReference type="EC" id="4.2.99.18" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323585.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXQ9"/>
    <dbReference type="STRING" id="348780.NP_3754A"/>
    <dbReference type="EnsemblBacteria" id="CAI49968">
        <property type="protein sequence ID" value="CAI49968"/>
        <property type="gene ID" value="NP_3754A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701856"/>
    <dbReference type="KEGG" id="nph:NP_3754A"/>
    <dbReference type="eggNOG" id="arCOG00464">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027543_3_0_2"/>
    <dbReference type="OrthoDB" id="14922at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008534">
        <property type="term" value="F:oxidized purine nucleobase lesion DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012904">
        <property type="entry name" value="OGG_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052054">
        <property type="entry name" value="Oxidative_DNA_repair_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10242">
        <property type="entry name" value="8-OXOGUANINE DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10242:SF2">
        <property type="entry name" value="N-GLYCOSYLASE_DNA LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07934">
        <property type="entry name" value="OGG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55945">
        <property type="entry name" value="TATA-box binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 11" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="10">
        <location>
            <begin position="135"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI49968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="302" mass="33322" checksum="8E51462DEF82AEA9" modified="2017-05-10" version="1">MDTGSRPIAAFDGGLDLQTTLESGQSYLWRREDGRMYDDAPAEGAWYYTVLPATATETNEPEVLRVRSVGDRLEWAASTPNAYERLEALLRLDDDLDAIFEATPDDPLVTSAYDAHRGLRLVCDPPFGTLISFICSAQMRVSRIHDMQAALADAYGETVVFDGTEYTAFPTPSALAAASEDALRDLSLGYRAPYVQRTAEMVANGDAHPTDAVGLAYEAARESLTRFVGVGEKVADCVLLFSLGYLEAVPLDTWIRTAIEDHYPDCARGNYADTSRAIRRQFGNEYAGYTQTYVFHHLRTGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXT9</accession>
    <name>A0A1U7EXT9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Flavin-containing amine-oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3824A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323620.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXT9"/>
    <dbReference type="STRING" id="348780.NP_3824A"/>
    <dbReference type="EnsemblBacteria" id="CAI50003">
        <property type="protein sequence ID" value="CAI50003"/>
        <property type="gene ID" value="NP_3824A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701875"/>
    <dbReference type="KEGG" id="nph:NP_3824A"/>
    <dbReference type="eggNOG" id="arCOG04623">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036034_0_0_2"/>
    <dbReference type="OrthoDB" id="203052at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16128">
        <property type="entry name" value="FAD/NAD(P)-BINDING OXIDOREDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16128:SF5">
        <property type="entry name" value="FAD_NAD(P)-BINDING OXIDOREDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="1">
        <location>
            <begin position="109"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="345" mass="37421" checksum="5D37EA123F465B3A" modified="2017-05-10" version="1">MVHIGIVGAGAAAAAATFVIDQTLPDAEVTVLEKSGGVCGRAATRRHDDLVYDYGANYVKSDDDRVVDLLTETLDTDGLVDVSEPIYTFDESGAVAPGRSADDHKWSYERGLTQVAKRLFGATDATVHRRTRVESLARTEEGWTIEDTDGDSWGPFDVVGLNPPAPQTAELLAATDWDDKRLATLQEAVKSVEYRPIWTAVLRYPFRLDRPYYALVNPGKDHDVGWIAREECKRGHVPDGESLLVVQASAQWSAANYDADPAENVAELASLTADIVGDERLAEPVWTDHQGWRYALPEDGVRRGPVSAAAEAGLYCLGDWVAGEGRLHAALAEGLAVGEQIVHAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXX4</accession>
    <name>A0A1U7EXX4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">YyaL family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3966A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323690.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXX4"/>
    <dbReference type="EnsemblBacteria" id="CAI50074">
        <property type="protein sequence ID" value="CAI50074"/>
        <property type="gene ID" value="NP_3966A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701925"/>
    <dbReference type="KEGG" id="nph:NP_3966A"/>
    <dbReference type="eggNOG" id="arCOG02007">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014051_4_2_2"/>
    <dbReference type="OrthoDB" id="28016at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02955">
        <property type="entry name" value="SSP411"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024705">
        <property type="entry name" value="Ssp411"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004879">
        <property type="entry name" value="Ssp411-like_TRX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42899">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42899:SF1">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03190">
        <property type="entry name" value="Thioredox_DsbH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006402">
        <property type="entry name" value="UCP006402_thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spermatogenesis-associated protein 20-like TRX" evidence="1">
        <location>
            <begin position="2"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="698" mass="77229" checksum="42B39D5433620B56" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY14</accession>
    <name>A0A1U7EY14_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4034A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY14"/>
    <dbReference type="STRING" id="348780.NP_4034A"/>
    <dbReference type="EnsemblBacteria" id="CAI50108">
        <property type="protein sequence ID" value="CAI50108"/>
        <property type="gene ID" value="NP_4034A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701948"/>
    <dbReference type="KEGG" id="nph:NP_4034A"/>
    <dbReference type="eggNOG" id="arCOG04328">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_923186_0_0_2"/>
    <dbReference type="OrthoDB" id="39107at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="269" mass="28968" checksum="4B7AC72897A2B387" modified="2017-05-10" version="1">MRLLVAGGAEVDAGKTTFTTGLVARTGVRGYKPRAGNGYWYDQDDYRRAVETERLYGKDAKRIAAANPDSAPPEAINPVHRLWMPTPGRGKGLLGREGRRFLLDRVTLDGDDSYVVNGDAELPPAATRAFPVDSSPHVESLPAFNDLMEQRHAPALSALADEIDRRGEAIVESYADIARPLSGFVPDAVAVVEPRRCRIYDGERYDKACSVASGSAREGQLEEHVGSVTELLDPAATVTLPALSSEERSDVGTVADAYAPAYDELLAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY29</accession>
    <name>A0A1U7EY29_NATPD</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4018A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323716.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY29"/>
    <dbReference type="STRING" id="348780.NP_4018A"/>
    <dbReference type="EnsemblBacteria" id="CAI50100">
        <property type="protein sequence ID" value="CAI50100"/>
        <property type="gene ID" value="NP_4018A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701942"/>
    <dbReference type="KEGG" id="nph:NP_4018A"/>
    <dbReference type="eggNOG" id="arCOG06117">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127456_0_0_2"/>
    <dbReference type="OrthoDB" id="125215at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056613">
        <property type="entry name" value="DUF7287"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23958">
        <property type="entry name" value="DUF7287"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="176" mass="18344" checksum="A712E356FB1899BE" modified="2017-05-10" version="1">MISVPTDRGQTTLDFAIGTSVFLLATIFVVAYVPTMFDPFAGGGGSKLVVADHAAATLSGDLLATSTAEPGTLSVGCVAAFFDAGPAEFCGTDFDDEEQMLALDDRNAAVTIHPLDASPTSPATLPETAIDDSHNVDSDADLAWTNAPDSQRQDVAVATRTVSVAGDQYRMTVEVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY39</accession>
    <name>A0A1U7EY39_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4098A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY39"/>
    <dbReference type="STRING" id="348780.NP_4098A"/>
    <dbReference type="EnsemblBacteria" id="CAI50140">
        <property type="protein sequence ID" value="CAI50140"/>
        <property type="gene ID" value="NP_4098A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_4098A"/>
    <dbReference type="eggNOG" id="arCOG10316">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_217790_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="39" mass="4576" checksum="2B20A81635FFC2E0" modified="2017-05-10" version="1">MNLILQAYEESPHIIDISGWVVLVASLLLTVGWLWYLYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY47</accession>
    <name>A0A1U7EY47_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable S-adenosylmethionine-dependent methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4122A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50152.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY47"/>
    <dbReference type="STRING" id="348780.NP_4122A"/>
    <dbReference type="EnsemblBacteria" id="CAI50152">
        <property type="protein sequence ID" value="CAI50152"/>
        <property type="gene ID" value="NP_4122A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701980"/>
    <dbReference type="KEGG" id="nph:NP_4122A"/>
    <dbReference type="eggNOG" id="arCOG02702">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037990_16_1_2"/>
    <dbReference type="OrthoDB" id="302307at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF110">
        <property type="entry name" value="RHODANESE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="1">
        <location>
            <begin position="43"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50152.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="186" mass="20051" checksum="6A9F220BBCF3005C" modified="2017-05-10" version="1">MGHHTFDSDRAAQLEEAGRRYRYLSRDELLGALAPDGDEVVADLGSGTGFYTDDIAPHVGGVHAVDIQEQMHEFYREKGVPENVSLVTSGIADLPFEPGALDAAVSTMTYHEFASDEALAELTRVLADGGRFVVADWRAGGNGEAGPPLDERYSATAAADALAAAGFERRREETRPETFLLVVQAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY90</accession>
    <name>A0A1U7EY90_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Acyl-CoA dehydrogenase</fullName>
            <ecNumber evidence="10">1.3.8.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">acd12</name>
        <name evidence="10" type="ordered locus">NP_4214A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.-" evidence="10"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50198.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323814.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY90"/>
    <dbReference type="STRING" id="348780.NP_4214A"/>
    <dbReference type="EnsemblBacteria" id="CAI50198">
        <property type="protein sequence ID" value="CAI50198"/>
        <property type="gene ID" value="NP_4214A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702476"/>
    <dbReference type="KEGG" id="nph:NP_4214A"/>
    <dbReference type="eggNOG" id="arCOG01707">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_3_5_2"/>
    <dbReference type="OrthoDB" id="275197at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF40">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0274">FAD</keyword>
    <keyword evidence="3 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="9">
        <location>
            <begin position="16"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="8">
        <location>
            <begin position="148"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="7">
        <location>
            <begin position="258"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI50198.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="406" mass="44176" checksum="5268762631DD1BCC" modified="2017-05-10" version="1">MSPHGAVSGVSFETDEETSLVLSSLDEFIEQEVKPLEADLGERWSNPRKRHEDDGRFVPDVLEAIETVRKKSAEAGFYAMNLPPEVGGDGVSNVTWYRAMRHVASNGPGLTEHVLAGPEGPKPLLAQAEGEQAERYLKPCVRGEKSTAFAQTEPGAGSDSPNMETTAEKDGDEWVLDGRKQWITNGPYADFVQVFARTTPQEELGRYGGITCFLVEDDEYEVSTLNNAVGMVGMQAELVFDAVRLPENRVLGTVDGAFYDAMEFLSLGRLEIGARALGHAEFLLDRGVEYAGDREAFGRPIGAFQGISHTLARGRARTFAADAAGLRLAWLLDRGEQAIEESSIVKYLATNVLFDVADGVVGVHGGNGVAEDNPFMNELQTARILRIVEGTDEIQLNTIAKELGVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYD7</accession>
    <name>A0A1U7EYD7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7979 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4332A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYD7"/>
    <dbReference type="STRING" id="348780.NP_4332A"/>
    <dbReference type="EnsemblBacteria" id="CAI50257">
        <property type="protein sequence ID" value="CAI50257"/>
        <property type="gene ID" value="NP_4332A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_4332A"/>
    <dbReference type="eggNOG" id="arCOG06367">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075998_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058285">
        <property type="entry name" value="DUF7979"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25934">
        <property type="entry name" value="DUF7979"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7979" evidence="1">
        <location>
            <begin position="225"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="310" mass="33565" checksum="4964DA4170C43491" modified="2017-05-10" version="1">MMPSISRRRLLVGGAAALGSALAGCPSPTPGGALEMHPAPDGTAVAEAATRSIDPERHRREAAVLDAALADTAAVITDERPPLSADRPVLYGATVYDVSVTEGPTDPVDRYLAVVSPSTNGRDRIEYEELPSIDRIALADLKRHEVELGDAAFDEDFEMAFDYYWYDEAALSASVLVPDPEYDVIVGTTAFNIAPQAETVTLTDFEYSATVRSDDPAAFGDRLAADATVVFEGLSDDERALVEDALDDGRTVVGLDNEAYVALGERLLAHDPIYETNRQGEWLVEYDGENYWVVLDPVRISELRDRLGDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYK5</accession>
    <name>A0A1U7EYK5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NUDIX family hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4494A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323953.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYK5"/>
    <dbReference type="STRING" id="348780.NP_4494A"/>
    <dbReference type="EnsemblBacteria" id="CAI50338">
        <property type="protein sequence ID" value="CAI50338"/>
        <property type="gene ID" value="NP_4494A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702079"/>
    <dbReference type="KEGG" id="nph:NP_4494A"/>
    <dbReference type="eggNOG" id="arCOG09021">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1727285_0_0_2"/>
    <dbReference type="OrthoDB" id="241167at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="153" mass="16622" checksum="213C58B6B099B04A" modified="2017-05-10" version="1">MNGSGPGAVSQYLDALYAEYDRFDVQQTTVAVTPSEFDELDDHGCHVSVRVHVKSDENGVLVRRDGDEWTLPGGEVTAEPLDAAAADLAERQTGVACEVRTLRQVAVVCLQCEPRDDEVWELSAEFVAEPVGGTPADRAEWQRGVDLEPISPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYN9</accession>
    <name>A0A1U7EYN9_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7124 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_4514A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323963.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYN9"/>
    <dbReference type="STRING" id="348780.NP_4514A"/>
    <dbReference type="EnsemblBacteria" id="CAI50348">
        <property type="protein sequence ID" value="CAI50348"/>
        <property type="gene ID" value="NP_4514A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702085"/>
    <dbReference type="KEGG" id="nph:NP_4514A"/>
    <dbReference type="eggNOG" id="arCOG03063">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150429_0_0_2"/>
    <dbReference type="OrthoDB" id="221805at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055548">
        <property type="entry name" value="DUF7124"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23439">
        <property type="entry name" value="DUF7124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7124" evidence="2">
        <location>
            <begin position="9"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="137" mass="15597" checksum="2F70961C926F7DE3" modified="2017-05-10" version="1">MNGGGSSDMTLAFELEALKELEDPEAVFSDARSWSEYVGVISEKPTYVVTNFTRKNRIRQDFFSGPRGREESLENVESQFQTERHVFVGTDEDDEALAESVDWEYLPITEAAEAAGWTLAEDEPESEPEPERRDDWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYP3</accession>
    <name>A0A1U7EYP3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">FMN-binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_4584A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323998.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYP3"/>
    <dbReference type="EnsemblBacteria" id="CAI50383">
        <property type="protein sequence ID" value="CAI50383"/>
        <property type="gene ID" value="NP_4584A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702105"/>
    <dbReference type="KEGG" id="nph:NP_4584A"/>
    <dbReference type="eggNOG" id="arCOG00514">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124266_0_0_2"/>
    <dbReference type="OrthoDB" id="288110at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024747">
        <property type="entry name" value="Pyridox_Oxase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12900">
        <property type="entry name" value="Pyridox_ox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="147" mass="16349" checksum="90449EB0A851C01C" modified="2017-05-10" version="1">MTADELPDDEVEALLRRANTGTLSLADGGDTYAVPQSFGYDGETLYFQLAYDDDSRKMEYIETTDTATFTVYEERPAQSVIVQGPFEPVPEGEETLATNAIAENAVIPTLNVSLDTPLDELRFDFYQLVPESLSGWRFHGFGEQPHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYU6</accession>
    <name>A0A1U7EYU6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">NP_4696A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324053.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="STRING" id="348780.NP_4696A"/>
    <dbReference type="EnsemblBacteria" id="CAI50439">
        <property type="protein sequence ID" value="CAI50439"/>
        <property type="gene ID" value="NP_4696A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702147"/>
    <dbReference type="KEGG" id="nph:NP_4696A"/>
    <dbReference type="eggNOG" id="arCOG02331">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02364">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG04809">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06408">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06796">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_257856_0_0_2"/>
    <dbReference type="OrthoDB" id="230688at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013656">
        <property type="entry name" value="PAS_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052162">
        <property type="entry name" value="Sensor_kinase/Photoreceptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43304:SF1">
        <property type="entry name" value="PAC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43304">
        <property type="entry name" value="PHYTOCHROME-LIKE PROTEIN CPH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08448">
        <property type="entry name" value="PAS_4"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="7">
        <location>
            <begin position="154"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="6">
        <location>
            <begin position="300"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="7">
        <location>
            <begin position="639"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="8">
        <location>
            <begin position="713"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="8">
        <location>
            <begin position="938"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="7">
        <location>
            <begin position="1005"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="8">
        <location>
            <begin position="1080"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="6">
        <location>
            <begin position="1141"/>
            <end position="1348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI50439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="1348" mass="150481" checksum="970934CBA9705FDD" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYX6</accession>
    <name>A0A1U7EYX6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Probable secreted glycoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">NP_4734A</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Cell wall</location>
            <location evidence="2">S-layer</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the halobacterial S-layer protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324071.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYX6"/>
    <dbReference type="EnsemblBacteria" id="CAI50458">
        <property type="protein sequence ID" value="CAI50458"/>
        <property type="gene ID" value="NP_4734A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702161"/>
    <dbReference type="KEGG" id="nph:NP_4734A"/>
    <dbReference type="eggNOG" id="arCOG06273">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006819_0_0_2"/>
    <dbReference type="OrthoDB" id="325633at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030115">
        <property type="term" value="C:S-layer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057149">
        <property type="entry name" value="DUF7827"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026371">
        <property type="entry name" value="PGF_CTERM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026452">
        <property type="entry name" value="Surf_glycop_sig_pep"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04207">
        <property type="entry name" value="halo_sig_pep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045517">
        <property type="entry name" value="halo_surf_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04126">
        <property type="entry name" value="PGF_CTERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25162">
        <property type="entry name" value="DUF7827"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18204">
        <property type="entry name" value="PGF-CTERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0134">Cell wall</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0701">S-layer</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="8 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="960"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7827" evidence="16">
        <location>
            <begin position="377"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="PGF-CTERM archaeal protein-sorting signal" evidence="15">
        <location>
            <begin position="961"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="909"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="916"/>
            <end position="958"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF18204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF25162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAI50458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="986" mass="107679" checksum="A4FB2B7839D28ED0" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EYZ1</accession>
    <name>A0A1U7EYZ1_NATPD</name>
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        <submittedName>
            <fullName evidence="3">Glutamate/valine-rich protein</fullName>
        </submittedName>
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        <name evidence="3" type="ordered locus">NP_4802A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
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            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
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            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EYZ1"/>
    <dbReference type="STRING" id="348780.NP_4802A"/>
    <dbReference type="EnsemblBacteria" id="CAI50492">
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        <property type="gene ID" value="NP_4802A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702181"/>
    <dbReference type="KEGG" id="nph:NP_4802A"/>
    <dbReference type="eggNOG" id="arCOG07580">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082636_1_0_2"/>
    <dbReference type="OrthoDB" id="206420at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="185" mass="20643" checksum="37A11117F2AE3B75" modified="2017-05-10" version="1">MLNPVRVDAIIDLTYGALIALSVVLIATFEPAAVGVAFGIGVFASYVVHVVWKMARFDPSWMTQAVEESVSETVGKQVEEVQDQVEKQVGDVQDQVEETVSETVEKQVEETVGETVEKQVEEAQAQVQETVEETVEETVEETVGETVEEVQEQVEAVDERVDRRPREEDIEEIVEESVDEETEQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ26</accession>
    <name>A0A1U7EZ26_NATPD</name>
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        <submittedName>
            <fullName evidence="4">UPF0045 family protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="4" type="ordered locus">NP_4848A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0045 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ26"/>
    <dbReference type="STRING" id="348780.NP_4848A"/>
    <dbReference type="EnsemblBacteria" id="CAI50515">
        <property type="protein sequence ID" value="CAI50515"/>
        <property type="gene ID" value="NP_4848A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702195"/>
    <dbReference type="KEGG" id="nph:NP_4848A"/>
    <dbReference type="eggNOG" id="arCOG04373">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137479_3_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029756">
        <property type="entry name" value="MTH1187/YkoF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002767">
        <property type="entry name" value="Thiamine_BP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051614">
        <property type="entry name" value="UPF0045_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00106">
        <property type="entry name" value="MTH1187 family thiamine-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33777">
        <property type="entry name" value="UPF0045 PROTEIN ECM15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33777:SF1">
        <property type="entry name" value="UPF0045 PROTEIN ECM15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01910">
        <property type="entry name" value="Thiamine_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89957">
        <property type="entry name" value="MTH1187/YkoF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiamine-binding protein" evidence="3">
        <location>
            <begin position="7"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="102" mass="10834" checksum="38B90D3BE61AB14F" modified="2017-05-10" version="1">MSVVALLSVAPVIEDSMASDVADAVAALDDFDVTYETNPMGTVIEADDIDTLLAAVGAAHKAVDADRVSTFLKVDDKRTTDQRAREKVEAVEAELGREPKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZB4</accession>
    <name>A0A1U7EZB4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Lrp/AsnC family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_5052A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324227.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZB4"/>
    <dbReference type="STRING" id="348780.NP_5052A"/>
    <dbReference type="EnsemblBacteria" id="CAI50617">
        <property type="protein sequence ID" value="CAI50617"/>
        <property type="gene ID" value="NP_5052A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702240"/>
    <dbReference type="KEGG" id="nph:NP_5052A"/>
    <dbReference type="eggNOG" id="arCOG01582">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_2_0_2"/>
    <dbReference type="OrthoDB" id="57033at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043200">
        <property type="term" value="P:response to amino acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154">
        <property type="entry name" value="LEUCINE-RESPONSIVE REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154:SF34">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR AZLB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="6"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI50617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="154" mass="17094" checksum="D31173ADBDD6706D" modified="2017-05-10" version="1">MTDYELDAVDREILYALQEEARNLSSSDIAERTDASSSTVRKRIQRLESEGVIKGYSANIDYTKSGYPIRMLLYCTAPIPERGEYIDDILDIPGVVSAQELVTGEQNLLVTAVGETDQDITPVAQQIADMGLTITDEVLVRSHRSTPFDDFSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZF8</accession>
    <name>A0A1U7EZF8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DUF2892 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_5194A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324298.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZF8"/>
    <dbReference type="EnsemblBacteria" id="CAI50688">
        <property type="protein sequence ID" value="CAI50688"/>
        <property type="gene ID" value="NP_5194A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702288"/>
    <dbReference type="KEGG" id="nph:NP_5194A"/>
    <dbReference type="eggNOG" id="arCOG06386">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_176022_2_2_2"/>
    <dbReference type="OrthoDB" id="100832at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021309">
        <property type="entry name" value="YgaP-like_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11127">
        <property type="entry name" value="YgaP-like_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="Inner membrane protein YgaP-like transmembrane" evidence="2">
        <location>
            <begin position="3"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="75" mass="7809" checksum="226392020610149A" modified="2017-05-10" version="1">MLEKNVGGLDRVARIGVGAILAVVGFSMLLAESLSILYGGVVLLVALVLLATAATQRCLLNKLLGADTTKLQSER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZI9</accession>
    <name>A0A1U7EZI9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TrmB family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5272A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324336.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZI9"/>
    <dbReference type="STRING" id="348780.NP_5272A"/>
    <dbReference type="EnsemblBacteria" id="CAI50727">
        <property type="protein sequence ID" value="CAI50727"/>
        <property type="gene ID" value="NP_5272A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702314"/>
    <dbReference type="KEGG" id="nph:NP_5272A"/>
    <dbReference type="eggNOG" id="arCOG02242">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140786_1_0_2"/>
    <dbReference type="OrthoDB" id="51378at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051797">
        <property type="entry name" value="TrmB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293:SF1">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator TrmB N-terminal" evidence="1">
        <location>
            <begin position="25"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="132" mass="15160" checksum="525D196D240DB619" modified="2017-05-10" version="1">MDPEPDPARFRDLMLDGEPGFEEVLRCVFGIQNHEARLYLELLEAPDSTVTELAETVDRDRSNVNRGLATLLEKGLADRRRRLLDSGGHVYQYRATDPETARELMHETLDEWAAYVHERIDEFPCATDGQSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZM0</accession>
    <name>A0A1U7EZM0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Hsp20-type molecular chaperone</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">hsp20D</name>
        <name evidence="5" type="ordered locus">NP_5318A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324359.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZM0"/>
    <dbReference type="STRING" id="348780.NP_5318A"/>
    <dbReference type="EnsemblBacteria" id="CAI50750">
        <property type="protein sequence ID" value="CAI50750"/>
        <property type="gene ID" value="NP_5318A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702832"/>
    <dbReference type="KEGG" id="nph:NP_5318A"/>
    <dbReference type="eggNOG" id="arCOG01836">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046737_10_0_2"/>
    <dbReference type="OrthoDB" id="210205at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06464">
        <property type="entry name" value="ACD_sHsps-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SHSP" evidence="4">
        <location>
            <begin position="23"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="120"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="143"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI50750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="152" mass="16087" checksum="6372C4254A80CDFD" modified="2017-05-10" version="1">MIRDLGESIGRTVLDGIGRAVSRVQEQRALSADLLESDEAYLAVFDAPGAMPEDIDVTFDDGTLDVRIDRVRGFHGDYEMRFPGRGQTLHGRVSLPDGAVVADEADATVTAHGTLEILLPKADGEDANGRDANGGGEGSDSDDSGKAERDDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZM8</accession>
    <name>A0A1U7EZM8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_5354A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324376.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZM8"/>
    <dbReference type="STRING" id="348780.NP_5354A"/>
    <dbReference type="EnsemblBacteria" id="CAI50768">
        <property type="protein sequence ID" value="CAI50768"/>
        <property type="gene ID" value="NP_5354A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702347"/>
    <dbReference type="KEGG" id="nph:NP_5354A"/>
    <dbReference type="HOGENOM" id="CLU_2821026_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="66" mass="6830" checksum="B08108E427BB9AB9" modified="2017-05-10" version="1">MTRATPTEMALTAVLAISALSWTVFGLFGFNILDAMGPEMTGGVAMLIGGIAIYDAADTFGIIDNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILU2</accession>
    <name>Q3ILU2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3300A</name>
        <name evidence="4" type="ordered locus">NP_7024A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4 5">
        <name>PL23</name>
    </geneLocation>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
            <strain evidence="4">Gabara</strain>
            <plasmid evidence="4">PL23</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CR936259">
        <property type="protein sequence ID" value="CAI50928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323362.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILU2"/>
    <dbReference type="STRING" id="348780.NP_3300A"/>
    <dbReference type="EnsemblBacteria" id="CAI49741">
        <property type="protein sequence ID" value="CAI49741"/>
        <property type="gene ID" value="NP_3300A"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI50928">
        <property type="protein sequence ID" value="CAI50928"/>
        <property type="gene ID" value="NP_7024A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703472"/>
    <dbReference type="KEGG" id="nph:NP_3300A"/>
    <dbReference type="KEGG" id="nph:NP_7024A"/>
    <dbReference type="HOGENOM" id="CLU_2230453_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="105" mass="11128" checksum="3B6A5AEFD14C6EC7" modified="2005-11-08" version="1">MTAGNDSDQPKTMLEYHFRRLSRKIVVVAGLVIASLFVAAGMIVTPFDSVVGLILTLAAIAVLAVCHAIMEDGLLRRGLNKLPGGSLSELKRPDEREPAGGVADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM02</accession>
    <name>Q3IM02_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ParA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">parA2</name>
        <name evidence="3" type="ordered locus">NP_6174A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324465.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM02"/>
    <dbReference type="EnsemblBacteria" id="CAI50867">
        <property type="protein sequence ID" value="CAI50867"/>
        <property type="gene ID" value="NP_6174A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694599"/>
    <dbReference type="KEGG" id="nph:NP_6174A"/>
    <dbReference type="HOGENOM" id="CLU_1003316_0_0_2"/>
    <dbReference type="OrthoDB" id="298117at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="CDD" id="cd02042">
        <property type="entry name" value="ParAB_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025669">
        <property type="entry name" value="AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050678">
        <property type="entry name" value="DNA_Partitioning_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13696:SF99">
        <property type="entry name" value="COBYRINIC ACID AC-DIAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13696">
        <property type="entry name" value="P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13614">
        <property type="entry name" value="AAA_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="9"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="292" mass="31891" checksum="F20EDB873C151200" modified="2005-11-08" version="1">MLAYTTYSEAGGVAKTTTAANLAVAHARAGLDVLVVPLDPQDGNLSHLLGVDDDRADPSVDNIVRHMVNRPKGPFEELIVTAESGVDIIPEHNMLSDLADNLRREKEQAEAMGESFGVNTRLLSVLRDAGVHETYDVLICDPPATEGRHLYNAITATHSLVIPVEPSAKGEASVKGLESLVAGIEDTLDIDVGVLAAVPVKWSNTNEAQRVLDELEYSTPEVFRSRESLMNGCWRQQCSAFTYVSEHRDRTRDHELDTLERYDRLARHLEQQAGVEPPAPPEPGALEPEVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM68</accession>
    <name>Q3IM68_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable secreted glycoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_6022A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324399.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM68"/>
    <dbReference type="EnsemblBacteria" id="CAI50791">
        <property type="protein sequence ID" value="CAI50791"/>
        <property type="gene ID" value="NP_6022A"/>
    </dbReference>
    <dbReference type="GeneID" id="43837308"/>
    <dbReference type="KEGG" id="nph:NP_6022A"/>
    <dbReference type="HOGENOM" id="CLU_098910_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="284" mass="30098" checksum="CB6A4A5284E9FA11" modified="2005-11-08" version="1">MSLPPTASRRRMLAAAGTALATFSLAGCSDESDDDDPEETEPEEPEPEPEPEPEPEPEPEPEPEPDDDGGDDSEDDDEPERSDDSALLSIIETLDGDEPLFDPGTETLSGDGDSVTDEISLGGALSVVIAEFDDEGTENYQLQLEGETDELLVNALEPGTFAGAVPTPSGDYLFDVTAPGGWELTVGQPLAPGEEIRTLPVNASGAGPDVVGPVELDGGMTVSGQHEGEENFQVHIYSEDNSDPFAGELVFNEIGEYEGEERADKSGVCWLDVEADGAWRLSID</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMG8</accession>
    <name>Q3IMG8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Probable phosphodiesterase</fullName>
            <ecNumber evidence="1">3.1.4.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_5198A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.4.-" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324300.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMG8"/>
    <dbReference type="STRING" id="348780.NP_5198A"/>
    <dbReference type="EnsemblBacteria" id="CAI50690">
        <property type="protein sequence ID" value="CAI50690"/>
        <property type="gene ID" value="NP_5198A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702290"/>
    <dbReference type="KEGG" id="nph:NP_5198A"/>
    <dbReference type="eggNOG" id="arCOG01377">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024306_1_0_2"/>
    <dbReference type="OrthoDB" id="33550at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="447" mass="50161" checksum="74DBC0B1E09A2574" modified="2005-11-08" version="1">MGLFDRLRGGDGPRVAFFGIDGVPYSLVADNEETFENLTAIAENGGGGAIDSIVPPESSACWPALTTGVNPGETGVYGFQDREVDSYDTYVPMGQDVQAKRLWTRVNDTGREATVLNVPVTFPPNRDIQRNASGFLSPGLKKAAYPDELRETLEDLDYKIDVNAKLGHKEDKSAFIEDAHRTLDARFEAFKHYIEQDDWHLFFGVFMTTDRVNHFLYKDYVTDGEYKEEFLEFYAKLDQYLGELRSLLSEETTMVVASDHGFMQLDHEVNCNVWLEENGWLSFDDDDHNSLSDIADDTRAYSLIPGRFYLNLKGREPNGTVPQDEYESVRSELKADLEAMEGPDGKPVADRVVTKEDAFRGDHDDIAPDLVVIPNTGFDLKSTFKGGEKVFTTGPRNGMHSFEDACLFVDDKQATIKNADLYDITPTILKLLDIETDRNEFDGASLV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-01-23" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMW7</accession>
    <name>RL30_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL30</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">50S ribosomal protein L30</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl30</name>
        <name type="ordered locus">NP_4896A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324151.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMW7"/>
    <dbReference type="SMR" id="Q3IMW7"/>
    <dbReference type="STRING" id="348780.NP_4896A"/>
    <dbReference type="EnsemblBacteria" id="CAI50539">
        <property type="protein sequence ID" value="CAI50539"/>
        <property type="gene ID" value="NP_4896A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703138"/>
    <dbReference type="KEGG" id="nph:NP_4896A"/>
    <dbReference type="eggNOG" id="arCOG04086">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055156_6_0_2"/>
    <dbReference type="OrthoDB" id="6379at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01657">
        <property type="entry name" value="Ribosomal_L7_archeal_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.15.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.20">
        <property type="entry name" value="Ribosomal protein L30, ferredoxin-like fold domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01371_A">
        <property type="entry name" value="Ribosomal_uL30_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036919">
        <property type="entry name" value="Ribo_uL30_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039699">
        <property type="entry name" value="Ribosomal_uL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005997">
        <property type="entry name" value="Ribosomal_uL30_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035808">
        <property type="entry name" value="Ribosomal_uL30_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_uL30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004711">
        <property type="entry name" value="PRK06049.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01309">
        <property type="entry name" value="uL30_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55129">
        <property type="entry name" value="Ribosomal protein L30p/L7e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000273911" description="Large ribosomal subunit protein uL30">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="114"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="123"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="154" mass="16773" checksum="F8F3C02972088E98" modified="2005-11-08" version="1">MKAIVQVRGEVNQEQGVRDTLEMLNLDRVNHATLVPETDAYDGMVTKVNDWVAHGEPSADVVATLIERRAEPAAGEGDIDDEWVADNTDYDGVEALAEALVDEETTLQEAGVSPTLRLHPPRGGHDGIKHPTKEGGQLGKHTTEEIDTLLEAMR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMX3</accession>
    <name>RS8_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein uS8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">30S ribosomal protein S8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps8</name>
        <name type="ordered locus">NP_4884A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324145.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMX3"/>
    <dbReference type="SMR" id="Q3IMX3"/>
    <dbReference type="STRING" id="348780.NP_4884A"/>
    <dbReference type="EnsemblBacteria" id="CAI50533">
        <property type="protein sequence ID" value="CAI50533"/>
        <property type="gene ID" value="NP_4884A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703187"/>
    <dbReference type="KEGG" id="nph:NP_4884A"/>
    <dbReference type="eggNOG" id="arCOG04091">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098428_1_1_2"/>
    <dbReference type="OrthoDB" id="5670at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01302_A">
        <property type="entry name" value="Ribosomal_uS8_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000630">
        <property type="entry name" value="Ribosomal_uS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047863">
        <property type="entry name" value="Ribosomal_uS8_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035987">
        <property type="entry name" value="Ribosomal_uS8_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003115">
        <property type="entry name" value="PRK04034.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11758">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S15A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
        <property type="entry name" value="Ribosomal_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56047">
        <property type="entry name" value="Ribosomal protein S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00053">
        <property type="entry name" value="RIBOSOMAL_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000225903" description="Small ribosomal subunit protein uS8">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="130" mass="14211" checksum="A09F9CBFE49EBBFE" modified="2005-11-08" version="1">MADNDPLASALSGLDNAESVGHLEQTIQPASNEIGSVLEVFYDRGYVDGFQFVDDGKAGRFEVELKGAINECGAVKPRYSAGADEFEKWEKRFLPARDYGTLIVTTSHGVMSHYEAREQGIGGQVIAYVY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IMY8</accession>
    <name>RL3_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">50S ribosomal protein L3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl3</name>
        <name type="ordered locus">NP_4854A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Forms a cluster with proteins L14 and L24e.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324130.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IMY8"/>
    <dbReference type="SMR" id="Q3IMY8"/>
    <dbReference type="STRING" id="348780.NP_4854A"/>
    <dbReference type="EnsemblBacteria" id="CAI50518">
        <property type="protein sequence ID" value="CAI50518"/>
        <property type="gene ID" value="NP_4854A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703137"/>
    <dbReference type="KEGG" id="nph:NP_4854A"/>
    <dbReference type="eggNOG" id="arCOG04070">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033361_2_0_2"/>
    <dbReference type="OrthoDB" id="6121at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.960.10">
        <property type="entry name" value="Ribosomal protein L3, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01325_A">
        <property type="entry name" value="Ribosomal_uL3_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045077">
        <property type="entry name" value="L3_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044892">
        <property type="entry name" value="Ribosomal_L3_dom_3_arc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000597">
        <property type="entry name" value="Ribosomal_uL3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019928">
        <property type="entry name" value="Ribosomal_uL3_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019926">
        <property type="entry name" value="Ribosomal_uL3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03626">
        <property type="entry name" value="L3_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003261">
        <property type="entry name" value="PRK04231.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11363">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11363:SF5">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00297">
        <property type="entry name" value="Ribosomal_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00474">
        <property type="entry name" value="RIBOSOMAL_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000241443" description="Large ribosomal subunit protein uL3">
        <location>
            <begin position="1"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="205"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="311"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="219"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="336" mass="36707" checksum="2B99187B945A97E2" modified="2005-11-08" version="1">MPQPSRPRKGSMGFSPRSRAASEVPRFNSWPDDEGQPGLQGFAGYKAGMSHVVAINDEPNSPREGQEETVPVTVVETPPMRAVAVRAYEDTPYGKRPLTEVWTDEVHEDLERSLSVPEEQSGDIEGDIRTALDEGALADVRVITHTVPGALSSVPKKEPDVMETRVGGGSLSDRVDFALDLVDDGGEHTVTDVFRAGEYTDVAGITKGKGTQGPVKRWGVQKRKGKHARQGWRRRIGNLGPWNPSRVRSTVPQQGQTGYHQRTELNKRLIDLGDDDVSPDGGFVNYGEVDGPYALVKGSVPGPDKRLVRFRPAVRPGDQPRLDPEVRYVSTASNQG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ING6</accession>
    <name>G1PDH_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycerol-1-phosphate dehydrogenase [NAD(P)+]</fullName>
            <shortName evidence="1">G1P dehydrogenase</shortName>
            <shortName evidence="1">G1PDH</shortName>
            <ecNumber evidence="1">1.1.1.261</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Enantiomeric glycerophosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">sn-glycerol-1-phosphate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">egsA</name>
        <name type="ordered locus">NP_4492A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NAD(P)H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol 1-phosphate (G1P). The G1P thus generated is used as the glycerophosphate backbone of phospholipids in the cellular membranes of Archaea.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>sn-glycerol 1-phosphate + NAD(+) = dihydroxyacetone phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21412"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.261"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>sn-glycerol 1-phosphate + NADP(+) = dihydroxyacetone phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21416"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.261"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycerol-1-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.261" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323952.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ING6"/>
    <dbReference type="SMR" id="Q3ING6"/>
    <dbReference type="STRING" id="348780.NP_4492A"/>
    <dbReference type="EnsemblBacteria" id="CAI50337">
        <property type="protein sequence ID" value="CAI50337"/>
        <property type="gene ID" value="NP_4492A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702751"/>
    <dbReference type="KEGG" id="nph:NP_4492A"/>
    <dbReference type="eggNOG" id="arCOG00982">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038362_0_0_2"/>
    <dbReference type="OrthoDB" id="8656at2157"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106357">
        <property type="term" value="F:glycerol-1-phosphate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106358">
        <property type="term" value="F:glycerol-1-phosphate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006650">
        <property type="term" value="P:glycerophospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08173">
        <property type="entry name" value="Gro1PDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1090.10">
        <property type="entry name" value="Dehydroquinate synthase-like - alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00497_A">
        <property type="entry name" value="G1P_dehydrogenase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023002">
        <property type="entry name" value="G1P_dehydrogenase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032837">
        <property type="entry name" value="G1PDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016205">
        <property type="entry name" value="Glycerol_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002022">
        <property type="entry name" value="PRK00843.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43616">
        <property type="entry name" value="GLYCEROL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43616:SF5">
        <property type="entry name" value="GLYCEROL DEHYDROGENASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13685">
        <property type="entry name" value="Fe-ADH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000112">
        <property type="entry name" value="Glycerol_dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56796">
        <property type="entry name" value="Dehydroquinate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_1000050605" description="Glycerol-1-phosphate dehydrogenase [NAD(P)+]">
        <location>
            <begin position="1"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="98"/>
            <end position="102"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="120"/>
            <end position="123"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00497"/>
        </source>
    </evidence>
    <sequence length="352" mass="37445" checksum="D6962B4807DAB534" modified="2005-11-08" version="1">MFEKSTWIRLPRSVVVGHGVLDRTAEAVEELYLDGRPLIVTSPTPEELAVDRLREQFVSAGFDPAVAVVEEASFDAVERVIETAERETAGFLVGFGGGKPIDIAKMAADERNCGFISVPTAASHDGIVSGRGSVPEGDTRHSVAAHPPLAVIADTELIANSPWRLTTAGCADIISNYTAVKDWQLAHRLKNVEYSEYAGALSQMTAEMLVDNSASIKRGLEESAWIVVKALVSSGVAMSIAGSSRPASGAEHLISHQLDRIAPETALHGHQVGVASIVTEYLHSGEGGDWRRVRDALASIDAPTTAAELGIDGERFIEATTSAHEIRDRYTILGDGIEREAAIEAAATTGVL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-02-10" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q3INN9</accession>
    <name>PELO_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein pelota homolog</fullName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pelA</name>
        <name type="ordered locus">NP_4344A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May function in recognizing stalled ribosomes, interact with stem-loop structures in stalled mRNA molecules, and effect endonucleolytic cleavage of the mRNA. May play a role in the release non-functional ribosomes and degradation of damaged mRNAs. Has endoribonuclease activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">The N-terminal domain has the RNA-binding Sm fold. It harbors the endoribonuclease activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic release factor 1 family. Pelota subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323879.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3INN9"/>
    <dbReference type="SMR" id="Q3INN9"/>
    <dbReference type="STRING" id="348780.NP_4344A"/>
    <dbReference type="EnsemblBacteria" id="CAI50263">
        <property type="protein sequence ID" value="CAI50263"/>
        <property type="gene ID" value="NP_4344A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703010"/>
    <dbReference type="KEGG" id="nph:NP_4344A"/>
    <dbReference type="eggNOG" id="arCOG01741">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023334_0_0_2"/>
    <dbReference type="OrthoDB" id="31300at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070966">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, no-go decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032790">
        <property type="term" value="P:ribosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071025">
        <property type="term" value="P:RNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.870:FF:000002">
        <property type="entry name" value="Protein pelota homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="entry name" value="eRF1 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.870">
        <property type="entry name" value="Pelota, domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01853">
        <property type="entry name" value="PelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_Pelota-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038069">
        <property type="entry name" value="Pelota/DOM34_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023521">
        <property type="entry name" value="Pelota_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058547">
        <property type="entry name" value="Pelota_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004405">
        <property type="entry name" value="TF_pelota"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00111">
        <property type="entry name" value="pelota"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853">
        <property type="entry name" value="PELOTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853:SF0">
        <property type="entry name" value="PROTEIN PELOTA HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26356">
        <property type="entry name" value="Pelota_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159065">
        <property type="entry name" value="Dom34/Pelota N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000361810" description="Protein pelota homolog">
        <location>
            <begin position="1"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01853"/>
        </source>
    </evidence>
    <sequence length="355" mass="39702" checksum="8E098C4B9471AEF5" modified="2005-11-08" version="1">MRIVDRESAEGRRERITLVPETLDDLWHLTYVLEPGDLVAGDTTRRIQRDDDKMRDTGGEREPMWIRIDVNNVEFAKFANRLRVGGDIVDCSREDQLGFHHTFNVEEHDELTVEKVWQVDQLERLEEAVEAAEQPDVAIATVEEGQAHIHTVAQYGVEERASITGTTGKGEYARSRDELFEELAAILRRLDAEAIILAGPGFTKQDALEHIEDNAPEAAEKIQVVDTASVGDRGVHEVLKRGAVDRIQTETRVSKEAELIDELMERIGEGEKAAYGVDEVAEAAEFGAIETLLILDERLREERAGEGDWAVDVNDIIENVEQQGGEVVVFSHEFDPGQQLANLGGIAALLRYRLS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IP73</accession>
    <name>PROB_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamate 5-kinase</fullName>
            <ecNumber evidence="1">2.7.2.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Gamma-glutamyl kinase</fullName>
            <shortName evidence="1">GK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">proB</name>
        <name type="ordered locus">NP_3976A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamate + ATP = L-glutamyl 5-phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:14877"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58274"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutamate 5-kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.11" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50079.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323695.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IP73"/>
    <dbReference type="SMR" id="Q3IP73"/>
    <dbReference type="STRING" id="348780.NP_3976A"/>
    <dbReference type="EnsemblBacteria" id="CAI50079">
        <property type="protein sequence ID" value="CAI50079"/>
        <property type="gene ID" value="NP_3976A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703055"/>
    <dbReference type="KEGG" id="nph:NP_3976A"/>
    <dbReference type="eggNOG" id="arCOG00864">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025400_0_0_2"/>
    <dbReference type="OrthoDB" id="142069at2157"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00359"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004349">
        <property type="term" value="F:glutamate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04242">
        <property type="entry name" value="AAK_G5K_ProB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1160.10:FF:000006">
        <property type="entry name" value="Glutamate 5-kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00456">
        <property type="entry name" value="ProB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041739">
        <property type="entry name" value="G5K_ProB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001057">
        <property type="entry name" value="Glu/AcGlu_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011529">
        <property type="entry name" value="Glu_5kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005715">
        <property type="entry name" value="Glu_5kinase/COase_Synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01027">
        <property type="entry name" value="proB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43654">
        <property type="entry name" value="GLUTAMATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43654:SF1">
        <property type="entry name" value="ISOPENTENYL PHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000729">
        <property type="entry name" value="GK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00474">
        <property type="entry name" value="GLU5KINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0641">Proline biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000230077" description="Glutamate 5-kinase">
        <location>
            <begin position="1"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="190"/>
            <end position="191"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00456"/>
        </source>
    </evidence>
    <sequence length="284" mass="30584" checksum="8E755E552D667BB1" modified="2005-11-08" version="1">MSETIADELGSVPQSVVEAAREDAANAKRVVVKAGTNSLTDEESNLDDDKLDKLVDDIADLLERDKDVLLVSSGAVGAGIGRVGYGSRTVEESQALSTVGQSHLMRRYTESFERYDRKVAQILLTQQDLENPERFTNFCNTVETLLEWDIVPIINENDAVATQEIRIGDNDMLSSSVAVGVDADLLVTLTDVDAVYTGNPKEDPDAERIEAVGENYDQIQGLVEESSSSDFGGIRTKVEGARNVSEYGIPAIIAGSAEPDVLQQIATGKSVGTLFVPINGETDD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-10-14" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IQS9</accession>
    <name>RS2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein uS2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">30S ribosomal protein S2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps2</name>
        <name type="ordered locus">NP_2848A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323140.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IQS9"/>
    <dbReference type="SMR" id="Q3IQS9"/>
    <dbReference type="STRING" id="348780.NP_2848A"/>
    <dbReference type="EnsemblBacteria" id="CAI49515">
        <property type="protein sequence ID" value="CAI49515"/>
        <property type="gene ID" value="NP_2848A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703175"/>
    <dbReference type="KEGG" id="nph:NP_2848A"/>
    <dbReference type="eggNOG" id="arCOG04245">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058171_3_0_2"/>
    <dbReference type="OrthoDB" id="371797at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01425">
        <property type="entry name" value="RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000030">
        <property type="entry name" value="30S ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00291_A">
        <property type="entry name" value="Ribosomal_uS2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_uS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023454">
        <property type="entry name" value="Ribosomal_uS2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018130">
        <property type="entry name" value="Ribosomal_uS2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005707">
        <property type="entry name" value="Ribosomal_uS2_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_uS2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01012">
        <property type="entry name" value="uS2_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11489">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN SA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00318">
        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00395">
        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="Ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00962">
        <property type="entry name" value="RIBOSOMAL_S2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00963">
        <property type="entry name" value="RIBOSOMAL_S2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000352075" description="Small ribosomal subunit protein uS2">
        <location>
            <begin position="1"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="277" mass="30268" checksum="3259E1C1E8586DDB" modified="2005-11-08" version="1">MSENDEGTDAAELDEELDELEDELEEDFEEPAAAADEADPTADEPPAEEAADEAADEAEADEPETDAEPADEEPVLDENVMPDDEADLLIPVEDYLAAGVHIGTQQKTKSMDRFIHRVRTDGLYVLDVSQTDQRIRTAASFLSNYQPEQILVASSRQYGRFPAEKFADAVGARARTGRFIPGTLTNPDYDGYIEPDVVVVTDPIGDSQAVKEAITVGIPVIAMCDSNNTTSNVDLVVPTNNKGRKALSVIYWLLANETLDRRGAEPAYALEDFETEP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-09-02" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IRZ2</accession>
    <name>URED_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Urease accessory protein UreD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ureD</name>
        <name type="ordered locus">NP_2016A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for maturation of urease via the functional incorporation of the urease nickel metallocenter.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">UreD, UreF and UreG form a complex that acts as a GTP-hydrolysis-dependent molecular chaperone, activating the urease apoprotein by helping to assemble the nickel containing metallocenter of UreC. The UreE protein probably delivers the nickel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UreD family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322728.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IRZ2"/>
    <dbReference type="SMR" id="Q3IRZ2"/>
    <dbReference type="STRING" id="348780.NP_2016A"/>
    <dbReference type="EnsemblBacteria" id="CAI49099">
        <property type="protein sequence ID" value="CAI49099"/>
        <property type="gene ID" value="NP_2016A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703451"/>
    <dbReference type="KEGG" id="nph:NP_2016A"/>
    <dbReference type="eggNOG" id="arCOG04529">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056339_1_0_2"/>
    <dbReference type="OrthoDB" id="10701at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01384">
        <property type="entry name" value="UreD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002669">
        <property type="entry name" value="UreD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33643">
        <property type="entry name" value="UREASE ACCESSORY PROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33643:SF1">
        <property type="entry name" value="UREASE ACCESSORY PROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01774">
        <property type="entry name" value="UreD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0996">Nickel insertion</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000346615" description="Urease accessory protein UreD">
        <location>
            <begin position="1"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01384"/>
        </source>
    </evidence>
    <sequence length="299" mass="31799" checksum="850292A06622D81D" modified="2005-11-08" version="1">MSAVPDAFQRYGAESLAQAPAFGPGKDGVFEATLARTGDRDTRLLRDYTKVPYHLTGTLDTDPAPGLTTLCLQEPTGGVAQGDRHSITVTAREGARARVTTQSATKVHSMQANYAHLDATLEAGPDAHLEYVPGPTIVNEDARCLQTVAVDLAPSATVVVADVFVPGGLSAHEPFDFDHYHSRLEARCEERLVCADAVDLHPADGDPRDPATVADYDVVGTLYVLAPEADTEVLAEAIHARFDAHDAVTAGVSALPYESGVSVRVLGTRSADVTDAVRDAWDASRQELLGVGIPADRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ISK5</accession>
    <name>Q3ISK5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Diphosphomevalonate decarboxylase</fullName>
            <ecNumber evidence="7">4.1.1.33</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">mvaD</name>
        <name evidence="10" type="ordered locus">NP_1580A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the diphosphomevalonate decarboxylase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.33" evidence="7"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322515.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ISK5"/>
    <dbReference type="STRING" id="348780.NP_1580A"/>
    <dbReference type="EnsemblBacteria" id="CAI48881">
        <property type="protein sequence ID" value="CAI48881"/>
        <property type="gene ID" value="NP_1580A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702959"/>
    <dbReference type="KEGG" id="nph:NP_1580A"/>
    <dbReference type="eggNOG" id="arCOG02937">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040369_0_0_2"/>
    <dbReference type="OrthoDB" id="275333at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004163">
        <property type="term" value="F:diphosphomevalonate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005935">
        <property type="entry name" value="Mev_decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029765">
        <property type="entry name" value="Mev_diP_decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049864">
        <property type="entry name" value="MvaD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053859">
        <property type="entry name" value="MVD-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041431">
        <property type="entry name" value="Mvd1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040704">
        <property type="entry name" value="IPP_alt_MvaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01240">
        <property type="entry name" value="mevDPdecarb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10977">
        <property type="entry name" value="DIPHOSPHOMEVALONATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10977:SF3">
        <property type="entry name" value="DIPHOSPHOMEVALONATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18376">
        <property type="entry name" value="MDD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22700">
        <property type="entry name" value="MVD-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015950">
        <property type="entry name" value="Mev_P_decrbx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Diphosphomevalonate decarboxylase-like N-terminal" evidence="9">
        <location>
            <begin position="7"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Mvd1 C-terminal" evidence="8">
        <location>
            <begin position="192"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="NCBIfam" id="TIGR01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF22700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAI48881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="324" mass="35472" checksum="7C6257CF8A0F3571" modified="2005-11-08" version="1">MKATAKAHPIQGLVKYHGMRNEELRLPYHDSISVCTAPSHSKTTAAFEPERDADRYVVDGEVVDGRGAERIRAVVDHVRDVADIDHRVRLESENDFPTNIGFGSSSSGFAAAAVALVEAAGLELSHPEISTVARRGSSSAARAVTGAFSQLYTGLDDADCYSERLDTDLEDDLRTVAAEIPAFKHTEEAHKEAADSHMFEARLAHIHEQIATMRNALRENDFDRVFELAEHDSLSLAATTMTGPAGWVYWQPETLSVFETVRTLREDDDIPVYFSTDTGASVYVNTTADHVDRVENAIAALGVDTRVWEVGGPAEVLPESESLF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ITB1</accession>
    <name>TBP_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TATA-box-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Box A-binding protein</fullName>
            <shortName evidence="1">BAP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">TATA sequence-binding protein</fullName>
            <shortName evidence="1">TBP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">TATA-box factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tbp</name>
        <name type="ordered locus">NP_1064A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">General factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Binds specifically to the TATA box promoter element which lies close to the position of transcription initiation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TBP family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322259.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ITB1"/>
    <dbReference type="SMR" id="Q3ITB1"/>
    <dbReference type="STRING" id="348780.NP_1064A"/>
    <dbReference type="EnsemblBacteria" id="CAI48623">
        <property type="protein sequence ID" value="CAI48623"/>
        <property type="gene ID" value="NP_1064A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703229"/>
    <dbReference type="KEGG" id="nph:NP_1064A"/>
    <dbReference type="eggNOG" id="arCOG01764">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060161_4_3_2"/>
    <dbReference type="OrthoDB" id="350539at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04518">
        <property type="entry name" value="TBP_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000007">
        <property type="entry name" value="TATA-box-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000010">
        <property type="entry name" value="TATA-box-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00408">
        <property type="entry name" value="TATA_bind_prot_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000814">
        <property type="entry name" value="TBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033711">
        <property type="entry name" value="TBP_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001593">
        <property type="entry name" value="PRK00394.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001595">
        <property type="entry name" value="PRK00394.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001597">
        <property type="entry name" value="PRK00394.2-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10126">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00352">
        <property type="entry name" value="TBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00686">
        <property type="entry name" value="TIFACTORIID"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55945">
        <property type="entry name" value="TATA-box binding protein-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00351">
        <property type="entry name" value="TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_1000049885" description="TATA-box-binding protein">
        <location>
            <begin position="1"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="repeat" description="1">
        <location>
            <begin position="10"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="repeat" description="2">
        <location>
            <begin position="101"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00408"/>
        </source>
    </evidence>
    <sequence length="187" mass="20409" checksum="9278561A594715DD" modified="2005-11-08" version="1">MQDPKETINIENVVASTGIGQELDLQSVAMDLEGADYDPEQFPGLVYRTQEPKSAALIFRSGKIVCTGAKSTDDVHESLRIVFDKLRDLEIQVDEDPEIVVQNIVTSADLGRNLNLNAIAIGLGLENIEYEPEQFPGLVYRLDDPDVVALLFGSGKLVITGGKEPDDAREAVDKIVSRLEELGLLDG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IU04</accession>
    <name>ENGB_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable GTP-binding protein EngB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">engB</name>
        <name type="ordered locus">NP_0576A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Necessary for normal cell division and for the maintenance of normal septation.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. EngB GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322015.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IU04"/>
    <dbReference type="SMR" id="Q3IU04"/>
    <dbReference type="STRING" id="348780.NP_0576A"/>
    <dbReference type="EnsemblBacteria" id="CAI48379">
        <property type="protein sequence ID" value="CAI48379"/>
        <property type="gene ID" value="NP_0576A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702673"/>
    <dbReference type="KEGG" id="nph:NP_0576A"/>
    <dbReference type="eggNOG" id="arCOG00355">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033732_3_0_2"/>
    <dbReference type="OrthoDB" id="65113at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01876">
        <property type="entry name" value="YihA_EngB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00321">
        <property type="entry name" value="GTPase_EngB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030393">
        <property type="entry name" value="G_ENGB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019987">
        <property type="entry name" value="GTP-bd_ribosome_bio_YsxC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003255">
        <property type="entry name" value="PRK04213.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11649:SF13">
        <property type="entry name" value="ENGB-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11649">
        <property type="entry name" value="MSS1/TRME-RELATED GTP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51706">
        <property type="entry name" value="G_ENGB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0717">Septation</keyword>
    <feature type="chain" id="PRO_0000266990" description="Probable GTP-binding protein EngB">
        <location>
            <begin position="1"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="EngB-type G" evidence="1">
        <location>
            <begin position="8"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="16"/>
            <end position="23"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="42"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="61"/>
            <end position="64"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="141"/>
            <end position="144"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="176"/>
            <end position="178"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00321"/>
        </source>
    </evidence>
    <sequence length="206" mass="23122" checksum="E137B5570B530200" modified="2005-11-08" version="1">MFETRPNRADEIVLVGRSNVGKSTLMREITGHDSFATGGSPGVTTKPNHYDWAGDDFVITDLPGFGFMSGVPEDVRESIKRDVVQYIEAHADNILAGILVVDGKAVVDIIDRHSGPDEIPHDVEMFGFLQELGIPTVVAVNKMDKVDDRDERLDELAERLGLYPPWQQFDEMLAPISAKRGSIEPLNQAVRHHLHEQKRDDLFKFF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IU43</accession>
    <name>MDH_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Malate dehydrogenase</fullName>
            <ecNumber evidence="1">1.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">mdh</name>
        <name type="ordered locus">NP_0498A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible oxidation of malate to oxaloacetate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-malate + NAD(+) = oxaloacetate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.37"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the LDH/MDH superfamily.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.37" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321976.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IU43"/>
    <dbReference type="SMR" id="Q3IU43"/>
    <dbReference type="STRING" id="348780.NP_0498A"/>
    <dbReference type="EnsemblBacteria" id="CAI48340">
        <property type="protein sequence ID" value="CAI48340"/>
        <property type="gene ID" value="NP_0498A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702903"/>
    <dbReference type="KEGG" id="nph:NP_0498A"/>
    <dbReference type="eggNOG" id="arCOG00246">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045401_1_1_2"/>
    <dbReference type="OrthoDB" id="2596at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004459">
        <property type="term" value="F:L-lactate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030060">
        <property type="term" value="F:L-malate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006089">
        <property type="term" value="P:lactate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="entry name" value="Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053411">
        <property type="entry name" value="MDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041314">
        <property type="entry name" value="Malate_DH_Halo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004863">
        <property type="entry name" value="PRK06223.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43128">
        <property type="entry name" value="L-2-HYDROXYCARBOXYLATE DEHYDROGENASE (NAD(P)(+))"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43128:SF16">
        <property type="entry name" value="L-LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00086">
        <property type="entry name" value="LLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="LDH C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="chain" id="PRO_0000241972" description="Malate dehydrogenase">
        <location>
            <begin position="1"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="8"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="119"/>
            <end position="121"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="O08349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P61889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="304" mass="32719" checksum="7F744512FA379A42" modified="2005-11-08" version="1">MTKVSIIGAAGTVGAAAGYNIALRDIADELVFVDIPDQRETTIGQAADANHGIAYDSNTTVVQGEYEDTAGSDVVVITAGIPRKEGQTRIDLAGDNAPIMEDIGASLDEYNDDYVSITTSNPVDLLNRHLYEAGDRDRHKVIGFGGRLDSARFRYVLSERFDVPVQNVEATILGEHGDAQVPVFSKVRVDGADPEFDGDEKEEILGDLQESAMDVISRKGATQWGPATGVAHMVEAVLNDTGEVLPGSLVLDGEYGYEDTAFGVPVKLGANGIEEVVEWDLDDYESELMDDAAEKLSDQYDKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETF5</accession>
    <name>A0A1U7ETF5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type transport system ATP-binding protein (Probable substrate branched-chain amino acids)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">livF5</name>
        <name evidence="7" type="synonym">abc09a1</name>
        <name evidence="7" type="ordered locus">NP_0166A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321813.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETF5"/>
    <dbReference type="STRING" id="348780.NP_0166A"/>
    <dbReference type="EnsemblBacteria" id="CAI48174">
        <property type="protein sequence ID" value="CAI48174"/>
        <property type="gene ID" value="NP_0166A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702392"/>
    <dbReference type="KEGG" id="nph:NP_0166A"/>
    <dbReference type="eggNOG" id="arCOG00924">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OrthoDB" id="97750at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015807">
        <property type="term" value="P:L-amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052156">
        <property type="entry name" value="BCAA_Transport_ATP-bd_LivF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43820">
        <property type="entry name" value="HIGH-AFFINITY BRANCHED-CHAIN AMINO ACID TRANSPORT ATP-BINDING PROTEIN LIVF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43820:SF4">
        <property type="entry name" value="HIGH-AFFINITY BRANCHED-CHAIN AMINO ACID TRANSPORT ATP-BINDING PROTEIN LIVF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="6"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI48174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="244" mass="26575" checksum="B70597E1BA1885E0" modified="2017-05-10" version="1">MSNPLLELTDNHAMVEGFEVTHGIDLTVEAGEAVALVGRNGAGKTSTFRGIMGLTPLDSGSVRFDGEELTDLRPEVIPMRGIGYQPEGRDLFTGMTVEENFRLPIWTSGDARGIDDEDAMVESIFDLFEELDDRRDAEVQNLSGGQGKMCAIGRAMALDPDLLLLDEPLEGLAPIVVENLKEYLREIIDRDISVLVAESNASHVPDIVDRMYVIERGEIVDSGDPEALLEDEEIQELMQGGGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETG2</accession>
    <name>A0A1U7ETG2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">UPF0761 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">NP_0192A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321826.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETG2"/>
    <dbReference type="STRING" id="348780.NP_0192A"/>
    <dbReference type="EnsemblBacteria" id="CAI48187">
        <property type="protein sequence ID" value="CAI48187"/>
        <property type="gene ID" value="NP_0192A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700784"/>
    <dbReference type="KEGG" id="nph:NP_0192A"/>
    <dbReference type="eggNOG" id="arCOG07870">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045539_1_1_2"/>
    <dbReference type="OrthoDB" id="202693at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017039">
        <property type="entry name" value="Virul_fac_BrkB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00765">
        <property type="entry name" value="yihY_not_rbn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30213">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YHJD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30213:SF0">
        <property type="entry name" value="UPF0761 MEMBRANE PROTEIN YIHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03631">
        <property type="entry name" value="Virul_fac_BrkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="134"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="165"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="200"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="227"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="369"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="395"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="270"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="274"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="292"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI48187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="435" mass="45218" checksum="AFE992BAA0E7C1EB" modified="2017-05-10" version="1">MKRHVDRTVAVTRGVVDGARSDRITFLAASLAYYGLVSLVPLLVLALVAASVVGEGQLIEALVVAVGDALGEQAGDVLGDALTGAAGRGGATLLGLAVFLWSGLKLFRGLDIAFSEVYGTAGPESLLSGIRNAVVTLGAVGLGVTATVAAGAAIAVLGVDAAVEGVALVGTLGLFVALTVAFLPLYYFLPGTDVSLSEAVPGAAFAAIGWTALQTGFRIYAGATDAYAAYGILGGVLLLVTFLYLGALVLLLGAVLNAVLAGRSPPSDPDSMTAAAGAAAPAAGRPPAPSADTTVPASAVTARSDTMTRDRPDDDASNEELREEIERLYDELDRFEERIEDRTVHRETLESDLRRYVRRRVRRGKARGWGPYLVLLYGTAMTLGAFYWLAGGWAVLAMVVIWLSTLGLYALMLIVGTTLTAAGLPGRLLDRVRDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETJ3</accession>
    <name>A0A1U7ETJ3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0276A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321868.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETJ3"/>
    <dbReference type="STRING" id="348780.NP_0276A"/>
    <dbReference type="EnsemblBacteria" id="CAI48229">
        <property type="protein sequence ID" value="CAI48229"/>
        <property type="gene ID" value="NP_0276A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700809"/>
    <dbReference type="KEGG" id="nph:NP_0276A"/>
    <dbReference type="eggNOG" id="arCOG15069">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2447751_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="89" mass="9240" checksum="F8868ADE4D33D410" modified="2017-05-10" version="1">MGLRRVLFAPLYRIADWADSNPLSAVGAIIALGALAMLLVSMSLSLEATGAELTTEAETAMLLAELAAERPAYLVTAGVGLAVVLFYDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7ETK7</accession>
    <name>A0A1U7ETK7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">CBS domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cbs2</name>
        <name evidence="4" type="ordered locus">NP_0326A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321892.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7ETK7"/>
    <dbReference type="STRING" id="348780.NP_0326A"/>
    <dbReference type="EnsemblBacteria" id="CAI48254">
        <property type="protein sequence ID" value="CAI48254"/>
        <property type="gene ID" value="NP_0326A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702580"/>
    <dbReference type="KEGG" id="nph:NP_0326A"/>
    <dbReference type="eggNOG" id="arCOG00600">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076812_1_0_2"/>
    <dbReference type="OrthoDB" id="85195at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd04614">
        <property type="entry name" value="CBS_pair_arch2_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="7"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="70"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="134"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="233"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="283" mass="30596" checksum="E8FA7F2FF83D9B1D" modified="2017-05-10" version="1">MNVVDAMTPRSEVVTVEIPGKREDVLEYLQERGFSSVPVVKSTADGELFRGLVTRESLIEQPDEDQLALLTREVPTVEPSASIAALAELVLESGERRVPVVEDDELVGIVTVTDIVRAIAEGDQDGATEVGSLATHEVNTIYQGTPLPVAERQLSYANVPYAVVLDDDGRQTGMLTEVDIIEVARVVQGETDPGDAVANQDSEWMWEGIKAVGSRAVPTRNVEIPSAAVAEFMTEDLISVPKHKTSRDAAQQMISNDIEQIPLVSGDELVGIVRDVDLLEALV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU27</accession>
    <name>A0A1U7EU27_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Small CPxCG-related zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0708A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="AlphaFoldDB" id="A0A1U7EU27"/>
    <dbReference type="STRING" id="348780.NP_0708A"/>
    <dbReference type="EnsemblBacteria" id="CAI48445">
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        <property type="gene ID" value="NP_0708A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700937"/>
    <dbReference type="KEGG" id="nph:NP_0708A"/>
    <dbReference type="eggNOG" id="arCOG06446">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_184177_0_0_2"/>
    <dbReference type="OrthoDB" id="190410at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055983">
        <property type="entry name" value="DUF7561"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24442">
        <property type="entry name" value="DUF7561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="69" mass="7138" checksum="A1B79B48E927AEB2" modified="2017-05-10" version="1">MASDPCEACGEDVQIGGGISGIWSSDPSGTGGMTLEFADGSAFFLCFDCIESLPEEPTVDDVRDLTDDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU35</accession>
    <name>A0A1U7EU35_NATPD</name>
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        <recommendedName>
            <fullName evidence="4">DUF445 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_0716A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
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            <taxon>Halobacteriales</taxon>
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            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48449.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="AlphaFoldDB" id="A0A1U7EU35"/>
    <dbReference type="STRING" id="348780.NP_0716A"/>
    <dbReference type="EnsemblBacteria" id="CAI48449">
        <property type="protein sequence ID" value="CAI48449"/>
        <property type="gene ID" value="NP_0716A"/>
    </dbReference>
    <dbReference type="GeneID" id="3700940"/>
    <dbReference type="KEGG" id="nph:NP_0716A"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028773_0_0_2"/>
    <dbReference type="OrthoDB" id="228250at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35791">
        <property type="entry name" value="UPF0754 MEMBRANE PROTEIN YHEB"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR35791:SF1">
        <property type="entry name" value="UPF0754 MEMBRANE PROTEIN YHEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="248"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="418"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48449.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="442" mass="49741" checksum="94CA48683E50EE9A" modified="2017-05-10" version="1">MSPLADTAATAADTVATAADAFEWRLLLIPLITGLIGYVTNWVAIRLLFQPVSFIGVRVPGVKSLAPILPRKLRQIPGVVEGRLGWQGIIPSRSARMGSIAAEKGVAKIASEREFYETFDPERITNHIVAHSDDEIRQLTDDILREEYPQLWASAPQPARELVYARVRERLPGVADEITDRIGEHIDELLDINAMITNHLEERPELLNRLFLEVGDRELKFIVNSGFLIGGFLGVFTVPLFVYIDRWWVLPACGVAVGYMTNWIALKIIFLPKRERRLGPFSLQGLFIKRQSEVAETYASIVADEIVTIGNVAENLLHGSQSDRTRQMIREAIRPEVDRTVSVAAPIIRVTSESQQYERLRETFADAGIDRTIQPLQDPQFNEERSEAIRELMTARIRELSPPDFVELLRPAFVEDEWMLILLGAVLGFVAGWLQLMVVTAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EU63</accession>
    <name>A0A1U7EU63_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1931 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_0780A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EU63"/>
    <dbReference type="STRING" id="348780.NP_0780A"/>
    <dbReference type="EnsemblBacteria" id="CAI48481">
        <property type="protein sequence ID" value="CAI48481"/>
        <property type="gene ID" value="NP_0780A"/>
    </dbReference>
    <dbReference type="GeneID" id="30959971"/>
    <dbReference type="GeneID" id="3700961"/>
    <dbReference type="KEGG" id="nph:NP_0780A"/>
    <dbReference type="eggNOG" id="arCOG02989">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_197986_1_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI48481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="55" mass="6224" checksum="A754DE9D2F8628A6" modified="2017-05-10" version="1">MADLIVKAAVKEALDDMNVASDFYDALDDEVEELLEDAARRAEENDRKTVQPRDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUD3</accession>
    <name>A0A1U7EUD3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NifU C-terminal domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_0948A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322201.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUD3"/>
    <dbReference type="STRING" id="348780.NP_0948A"/>
    <dbReference type="EnsemblBacteria" id="CAI48565">
        <property type="protein sequence ID" value="CAI48565"/>
        <property type="gene ID" value="NP_0948A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701007"/>
    <dbReference type="KEGG" id="nph:NP_0948A"/>
    <dbReference type="eggNOG" id="arCOG03026">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151086_1_0_2"/>
    <dbReference type="OrthoDB" id="270036at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001075">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01106">
        <property type="entry name" value="NifU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NIF system FeS cluster assembly NifU C-terminal" evidence="2">
        <location>
            <begin position="25"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI48565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="121" mass="12764" checksum="CE820A57083D40B6" modified="2017-05-10" version="1">MSTETADGDEELRERISNFLRRNFPQIQMHGGSAAIQDLDRENGEVTIMLGGACSGCGISPMTIQAIKSRMTEEIPEIDTVYADTGSDGGSEGMAGGQVSPSFPGETTSDDDSDEGPDAPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUJ2</accession>
    <name>A0A1U7EUJ2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HTH domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1128A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322291.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUJ2"/>
    <dbReference type="STRING" id="348780.NP_1128A"/>
    <dbReference type="EnsemblBacteria" id="CAI48655">
        <property type="protein sequence ID" value="CAI48655"/>
        <property type="gene ID" value="NP_1128A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701058"/>
    <dbReference type="KEGG" id="nph:NP_1128A"/>
    <dbReference type="eggNOG" id="arCOG02611">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109676_0_0_2"/>
    <dbReference type="OrthoDB" id="156316at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056504">
        <property type="entry name" value="HTH_HVO_0163_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36216:SF1">
        <property type="entry name" value="HTH ARSR-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36216">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, TRMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24266">
        <property type="entry name" value="HTH_HVO_0163_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HVO-0163 N-terminal HTH" evidence="1">
        <location>
            <begin position="37"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="202" mass="22163" checksum="1CACE1ADFC25F940" modified="2017-05-10" version="1">MTDGGIDEGKRATLKRFAALGATTPLVRLSADDGGENETRDAIVGYLATTPGAHFSKLRDDLQLGTGETQHHLRTLEEDGTIEHRKDGEYKRYFPAGRFSEHEQRALGYLRRETPRRMLIELLSDPQISGSDIADALGVSRSTVSKYAAELEAAGLLDREDGYTVRQPETLVALVVRYADSFGPAAVAFADTVDEYVSYDPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUP0</accession>
    <name>A0A1U7EUP0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Probable phosphoribosyltransferase</fullName>
            <ecNumber evidence="4">2.4.2.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">gptA</name>
        <name evidence="4" type="ordered locus">NP_1204A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.4.2.-" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322329.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUP0"/>
    <dbReference type="STRING" id="348780.NP_1204A"/>
    <dbReference type="EnsemblBacteria" id="CAI48693">
        <property type="protein sequence ID" value="CAI48693"/>
        <property type="gene ID" value="NP_1204A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701081"/>
    <dbReference type="KEGG" id="nph:NP_1204A"/>
    <dbReference type="eggNOG" id="arCOG00040">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080904_0_0_2"/>
    <dbReference type="OrthoDB" id="4952at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43363">
        <property type="entry name" value="HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43363:SF2">
        <property type="entry name" value="PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="3">
        <location>
            <begin position="17"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="230" mass="26089" checksum="D395834152ABCDAE" modified="2017-05-10" version="1">MSDLPDEFNCTVTNWEYIYSLCRDVADHVKAAEFEPDVVVALARGGWFAGRCLCDFLGLDDLTSLKMEHYVGTGQKSDEPQVRYPMPEGSVEGKNVLVIDDIADTGGSISRAYEYVTERDAAEVRTATLQLLDTSEFEPEFVGERLETWTWVVYPWNFIEDMIELIGGVMEKADADTFSRDDIRHYLSAYHGLERIEMEIAQPDRLDEVLAEMERRGAARKVGDDAWQPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EUW3</accession>
    <name>A0A1U7EUW3_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">IMPACT family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">NP_1410A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IMPACT family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322431.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EUW3"/>
    <dbReference type="STRING" id="348780.NP_1410A"/>
    <dbReference type="EnsemblBacteria" id="CAI48796">
        <property type="protein sequence ID" value="CAI48796"/>
        <property type="gene ID" value="NP_1410A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701141"/>
    <dbReference type="KEGG" id="nph:NP_1410A"/>
    <dbReference type="eggNOG" id="arCOG03107">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083552_1_1_2"/>
    <dbReference type="OrthoDB" id="121633at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006446">
        <property type="term" value="P:regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.30">
        <property type="entry name" value="Impact, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023582">
        <property type="entry name" value="Impact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001498">
        <property type="entry name" value="Impact_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036956">
        <property type="entry name" value="Impact_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015269">
        <property type="entry name" value="UPF0029_Impact_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020569">
        <property type="entry name" value="UPF0029_Impact_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16301:SF20">
        <property type="entry name" value="IMPACT FAMILY MEMBER YIGZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16301">
        <property type="entry name" value="IMPACT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09186">
        <property type="entry name" value="DUF1949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01205">
        <property type="entry name" value="Impact_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00910">
        <property type="entry name" value="UPF0029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Impact N-terminal" evidence="2">
        <location>
            <begin position="22"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="UPF0029" evidence="3">
        <location>
            <begin position="144"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI48796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="199" mass="21349" checksum="022FAB0A7E46E4F9" modified="2017-05-10" version="1">MSSTDAYRTIDGRARSAFTVQGSEFIGHAAPVETVAKAEAFIDDVAAEHADATHNVPAYRVRADPFREYSSDDGEPSGSAGKPMLSVLGGRELENVCVVVTRYFGGTKLGVGGLVRAYSKATKDVLDAAEVVEEVPRERFEAVVEYDDSGTIRGILESEDVEFDAAYGADVVFDISAPVAEAEHLRDRLRSATSGRVDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV06</accession>
    <name>A0A1U7EV06_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="13">HtpX-like protease</fullName>
            <ecNumber evidence="13">3.4.24.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">htpX2</name>
        <name evidence="13" type="ordered locus">NP_1484A</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the peptidase M48 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="13"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322468.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV06"/>
    <dbReference type="STRING" id="348780.NP_1484A"/>
    <dbReference type="EnsemblBacteria" id="CAI48833">
        <property type="protein sequence ID" value="CAI48833"/>
        <property type="gene ID" value="NP_1484A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702835"/>
    <dbReference type="KEGG" id="nph:NP_1484A"/>
    <dbReference type="eggNOG" id="arCOG01331">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042266_0_2_2"/>
    <dbReference type="OrthoDB" id="28389at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.10">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050083">
        <property type="entry name" value="HtpX_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43221">
        <property type="entry name" value="PROTEASE HTPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43221:SF2">
        <property type="entry name" value="PROTEASE HTPX HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 10 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
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    <keyword evidence="2 10 13" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
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            <end position="54"/>
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            <end position="166"/>
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    </feature>
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            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
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            <begin position="72"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU003983"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI48833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="279" mass="30456" checksum="299B038E81F1D439" modified="2017-05-10" version="1">MRHAGLKARMAVVGSILFGFYALLALIAYYGFGIGLELIFLGAIAFAGFQYIVGKKAALWSSGAEDMPEDKYPEIHRSVERISDEMDLEKPRLMVADMGVPNAFAVGRRGAGVVVVSEQLIQLLDHDELEAVLAHELAHIDNRDVIMMVIGQSIASMLGLAVQFAILFTRDGGIGNFLLAYVAGIITQMIAMVFVLAISRYREYVADSDAAQHVGGDAMARALHKISSVGERQEAEMDDNVSALCIFGGEKSALQKLFATHPPIEKRIEAVQSVERQHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EV82</accession>
    <name>A0A1U7EV82_NATPD</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_1674A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EV82"/>
    <dbReference type="STRING" id="348780.NP_1674A"/>
    <dbReference type="EnsemblBacteria" id="CAI48928">
        <property type="protein sequence ID" value="CAI48928"/>
        <property type="gene ID" value="NP_1674A"/>
    </dbReference>
    <dbReference type="GeneID" id="77383459"/>
    <dbReference type="KEGG" id="nph:NP_1674A"/>
    <dbReference type="eggNOG" id="arCOG09225">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_204991_1_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI48928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="43" mass="4698" checksum="6BAB1320F13433DC" modified="2017-05-10" version="1">MDTAQFKHPALQSIGGTVVAYALIIFVMTALLFGVPYLLFLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVH3</accession>
    <name>A0A1U7EVH3_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">NP_1882A</name>
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    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
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            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
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            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
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    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="348780.NP_1882A"/>
    <dbReference type="EnsemblBacteria" id="CAI49032">
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        <property type="gene ID" value="NP_1882A"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_1882A"/>
    <dbReference type="eggNOG" id="arCOG02327">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_328084_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004673">
        <property type="term" value="F:protein histidine kinase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="2C_sensor_his_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031621">
        <property type="entry name" value="HisKA_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44936">
        <property type="entry name" value="SENSOR PROTEIN CREC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44936:SF10">
        <property type="entry name" value="SENSOR PROTEIN RSTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16927">
        <property type="entry name" value="HisKA_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13188">
        <property type="entry name" value="PAS_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="185"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="9">
        <location>
            <begin position="520"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="10">
        <location>
            <begin position="591"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="8">
        <location>
            <begin position="758"/>
            <end position="862"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI49032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="876" mass="95120" checksum="9E0B0B2689FA12E3" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVJ6</accession>
    <name>A0A1U7EVJ6_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">NP_1914A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
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    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
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            <dbReference type="PROSITE" id="PS50113"/>
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            <dbReference type="EMBL" id="CAI49048.3"/>
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        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
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    <sequence length="471" mass="51514" checksum="439B914EFE934EF9" modified="2017-05-10" version="1">MNERLYRQVVEESNDGIRVVQDGTVVFVNERLCEMTGYDEAEILGTSKADIVAPEYKQTVEAHHHARVAGEPAPSQYEVELETAGGERLPVELSATRIDYEGEPASLVLHRDISQRKREEAATEQARAQLRQVIDLVPDPVFAIDGDGEYVLVNEAMATLFGTTPQAMVGETPAALGRPALVDRLHDSDESVLESGDRLERAAVELPRDDGADMTVQLTKIPYTVAGTGEKAVLGYARDITARLASEERLETQRDNLELLNQVVRHDIRNDLQLVTAYAEILEDHVDEAGQEHLETVVENATSAVELTRTARDVAAVVLRSDADRAPVDLGRVIEDQLAEVRSNYERAVVTTDGPVPSVTVLADDMLASVVRNLLTNAIEHNDTEPPTVTVSVTVAGEQAVVRVADDGPGIPDNRKEAIFERNERGLDSDGTGLGLYLVETLVNRYDGSIRVEDNDPRGSVFVVELPTIES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVM0</accession>
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        <name evidence="3" type="primary">ureF</name>
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        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49101.1"/>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
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    <dbReference type="EnsemblBacteria" id="CAI49101">
        <property type="protein sequence ID" value="CAI49101"/>
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    <dbReference type="GeneID" id="3703453"/>
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        <property type="taxonomic scope" value="Archaea"/>
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        <property type="component" value="Chromosome"/>
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        <property type="term" value="F:nickel cation binding"/>
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        <property type="entry name" value="Urease accessory protein UreF"/>
        <property type="match status" value="1"/>
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    <dbReference type="HAMAP" id="MF_01385">
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        <property type="entry name" value="UreF"/>
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        <property type="entry name" value="UreF_sf"/>
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        <property type="entry name" value="UREASE ACCESSORY PROTEIN F"/>
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        <property type="entry name" value="Ureas_acces_UreF"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
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    <evidence type="ECO:0000256" key="1">
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="237" mass="25027" checksum="95FDF35468FF0F4D" modified="2017-05-10" version="1">MSETSFLTALRLADSFLPVGTYTASYGIEQYVNEGDIETAAELGDLVEAYLRRIVGPAETVALAAAHRAAAADDEAGIRAADERLHAATLPAEFRDSATKAGTKLLELLASTDEGLFAETTLDGAVAAYAAAVAADETPGQYPVVLGVVTQRAGLGRREACLVGAYSVVTELLGAAQRLGRFGHTDIQSQLAELLPVIEEVCDEYVDAELSSLSSFAPLAETMGMAHERADRRLFMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EVV4</accession>
    <name>A0A1U7EVV4_NATPD</name>
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        <submittedName>
            <fullName evidence="5">Fla cluster protein FlaCE</fullName>
        </submittedName>
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        <name evidence="5" type="primary">flaCE</name>
        <name evidence="5" type="ordered locus">NP_2154A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Archaeal flagellum</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A1U7EVV4"/>
    <dbReference type="STRING" id="348780.NP_2154A"/>
    <dbReference type="EnsemblBacteria" id="CAI49168">
        <property type="protein sequence ID" value="CAI49168"/>
        <property type="gene ID" value="NP_2154A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702708"/>
    <dbReference type="KEGG" id="nph:NP_2154A"/>
    <dbReference type="eggNOG" id="arCOG02963">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030327_0_0_2"/>
    <dbReference type="OrthoDB" id="308199at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097589">
        <property type="term" value="C:archaeal-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="P:archaeal or bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006752">
        <property type="entry name" value="Arch_fla_DE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009205">
        <property type="entry name" value="FlaC_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052494">
        <property type="entry name" value="Flagella_assembly_related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40698:SF1">
        <property type="entry name" value="FLAGELLA-RELATED PROTEIN D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40698">
        <property type="entry name" value="FLAGELLA-RELATED PROTEIN E-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04659">
        <property type="entry name" value="Arch_fla_DE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05377">
        <property type="entry name" value="FlaC_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0974">Archaeal flagellum</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Archaeal flagella protein FlaD/E" evidence="4">
        <location>
            <begin position="363"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="136"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="465"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="45"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="143"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="158"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="269"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="465"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="477"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="488"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="531"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49168.1"/>
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</entry>
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</entry>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
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                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
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            <dbReference type="PubMed" id="16169924"/>
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            <dbReference type="EMBL" id="CAI49363.1"/>
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</entry>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
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                <person name="Pfeiffer F."/>
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                <person name="Rodewald K."/>
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            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
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        <property type="entry name" value="BLL3936 PROTEIN"/>
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    </dbReference>
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    </dbReference>
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        <location>
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            <end position="55"/>
        </location>
    </feature>
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        <location>
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    </feature>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="Pfam" id="PF06808"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAI49403.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWE4</accession>
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        <submittedName>
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        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
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                <person name="Pfeiffer F."/>
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                <person name="Rodewald K."/>
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            <dbReference type="PubMed" id="16169924"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
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        <source>
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    </evidence>
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        </source>
    </evidence>
    <sequence length="147" mass="15578" checksum="91D0E3B5EFC12F8E" modified="2017-05-10" version="1">MGEYRVLLPVDTETNRARHAAETVAALPGDGASVHATVLHVIEPFEATDEGPIIETDDIHTDDTLPDSIETAADALRSEGVEVDTRHVEGDPVETILGVARDLDVDSIVMCGRRRSPAGKVLFGSTVQSVLLAAERPVVTVAAEDGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWG5</accession>
    <name>A0A1U7EWG5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transport protein (Probable substrate cationic amino acids)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cat3</name>
        <name evidence="8" type="synonym">tp06</name>
        <name evidence="8" type="ordered locus">NP_2676A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323054.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWG5"/>
    <dbReference type="STRING" id="348780.NP_2676A"/>
    <dbReference type="EnsemblBacteria" id="CAI49429">
        <property type="protein sequence ID" value="CAI49429"/>
        <property type="gene ID" value="NP_2676A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703283"/>
    <dbReference type="KEGG" id="nph:NP_2676A"/>
    <dbReference type="eggNOG" id="arCOG00009">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_15_8_2"/>
    <dbReference type="OrthoDB" id="43026at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050367">
        <property type="entry name" value="APC_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770">
        <property type="entry name" value="AMINO ACID TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770:SF7">
        <property type="entry name" value="MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="81"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="118"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="227"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="262"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="324"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="347"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="380"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="405"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="UspA" evidence="7">
        <location>
            <begin position="464"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI49429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="736" mass="76497" checksum="3E2926FBC6A496D7" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWL2</accession>
    <name>A0A1U7EWL2_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribulose bisphosphate carboxylase</fullName>
            <shortName evidence="6">RuBisCO</shortName>
            <ecNumber evidence="6">4.1.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 9" type="primary">rbcL</name>
        <name evidence="9" type="ordered locus">NP_2770A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the addition of molecular CO(2) and H(2)O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2 (2R)-3-phosphoglycerate + 2 H(+) = D-ribulose 1,5-bisphosphate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:23124"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57870"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="EC" id="4.1.1.39"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>D-ribulose 1,5-bisphosphate + O2 = 2-phosphoglycolate + (2R)-3-phosphoglycerate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:36631"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57870"/>
            <dbReference type="ChEBI" id="CHEBI:58033"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="6">Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O(2) to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the RuBisCO large chain family. Type III subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.39" evidence="6"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323101.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWL2"/>
    <dbReference type="STRING" id="348780.NP_2770A"/>
    <dbReference type="EnsemblBacteria" id="CAI49476">
        <property type="protein sequence ID" value="CAI49476"/>
        <property type="gene ID" value="NP_2770A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703097"/>
    <dbReference type="KEGG" id="nph:NP_2770A"/>
    <dbReference type="eggNOG" id="arCOG04443">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031450_3_1_2"/>
    <dbReference type="OrthoDB" id="52787at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016984">
        <property type="term" value="F:ribulose-bisphosphate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006196">
        <property type="term" value="P:AMP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015977">
        <property type="term" value="P:carbon fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.110">
        <property type="entry name" value="Ribulose bisphosphate carboxylase, large subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.150">
        <property type="entry name" value="RuBisCO large subunit, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01133">
        <property type="entry name" value="RuBisCO_L_type3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033966">
        <property type="entry name" value="RuBisCO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017712">
        <property type="entry name" value="RuBisCO_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000685">
        <property type="entry name" value="RuBisCO_lsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036376">
        <property type="entry name" value="RuBisCO_lsu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017443">
        <property type="entry name" value="RuBisCO_lsu_fd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036422">
        <property type="entry name" value="RuBisCO_lsu_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003252">
        <property type="entry name" value="PRK04208.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03326">
        <property type="entry name" value="rubisco_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42704">
        <property type="entry name" value="RIBULOSE BISPHOSPHATE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42704:SF17">
        <property type="entry name" value="RIBULOSE BISPHOSPHATE CARBOXYLASE LARGE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00016">
        <property type="entry name" value="RuBisCO_large"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02788">
        <property type="entry name" value="RuBisCO_large_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00014">
        <property type="entry name" value="RuBisCO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00301">
        <property type="entry name" value="RuBisCO-like_proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51649">
        <property type="entry name" value="RuBisCo, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54966">
        <property type="entry name" value="RuBisCO, large subunit, small (N-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0120">Carbon dioxide fixation</keyword>
    <keyword evidence="4 6 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribulose bisphosphate carboxylase large subunit ferrodoxin-like N-terminal" evidence="8">
        <location>
            <begin position="5"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Ribulose bisphosphate carboxylase large subunit C-terminal" evidence="7">
        <location>
            <begin position="137"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="271"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="via carbamate group" evidence="6">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="340"/>
            <end position="342"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="362"/>
            <end position="365"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="6">
        <location>
            <position position="312"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="6">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI49476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="417" mass="44911" checksum="0DF3D4C18F33A6EC" modified="2017-05-10" version="1">MEYADFLDESYEPSDDDLVCTFRLVPGEGISVADAAARVASESSNGTWAALSPESDVRQYSALACDIGPEDEHGTQVTVAYPSGLFEDGSLPQILSCIAGNIMGMKAVETIRLLDCEWPAVIARSFPGPQYGSDVRTELLDAGDRPPLATVPKPKVGLSTEEHVSVAESAWRGGVDLLKDDENLTDQTFNPFEQRVADSFAARDRLEEETGERKDYLVNITAETDEMVRRAEFVDDHGGSFVMVDIITCGWSGLQTVRRRTEDLDLAIHAHRAMHAAFDRLPQHGVSMRCLAQFARLAGVDHIHTGTAGLGKLENEDTAGINEWLRSDLHGHSDVLPVASGGLHPGIVDQLLDALGTNVMVQAGGGIHGHPDGTEAGARALRAAVDAYADGESLDSRAESVPALRTALDEWGTQNPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWN2</accession>
    <name>A0A1U7EWN2_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">HxlR family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">NP_2812A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323122.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWN2"/>
    <dbReference type="STRING" id="348780.NP_2812A"/>
    <dbReference type="EnsemblBacteria" id="CAI49497">
        <property type="protein sequence ID" value="CAI49497"/>
        <property type="gene ID" value="NP_2812A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701566"/>
    <dbReference type="KEGG" id="nph:NP_2812A"/>
    <dbReference type="eggNOG" id="arCOG01057">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111585_5_3_2"/>
    <dbReference type="OrthoDB" id="147589at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002577">
        <property type="entry name" value="HTH_HxlR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33204">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, MARR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33204:SF18">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01638">
        <property type="entry name" value="HxlR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51118">
        <property type="entry name" value="HTH_HXLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH hxlR-type" evidence="4">
        <location>
            <begin position="12"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="108" mass="12609" checksum="25C28DD12AB72E3C" modified="2017-05-10" version="1">MSNTQRRLEAACPVVESMDQIGSKWRLLVLHDLQHGEKRFNELKRSTGANSRTLSRVLDDLQETEFVERRLEEDAPVATYYSLTQKGRSLAPVFDEIEEWADEWLEIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWQ4</accession>
    <name>A0A1U7EWQ4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC-type transport system periplasmic substrate-binding protein (Probable substrate zinc)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">znuA1</name>
        <name evidence="5" type="synonym">abc19s</name>
        <name evidence="5" type="ordered locus">NP_2862A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial solute-binding protein 9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323147.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWQ4"/>
    <dbReference type="STRING" id="348780.NP_2862A"/>
    <dbReference type="EnsemblBacteria" id="CAI49522">
        <property type="protein sequence ID" value="CAI49522"/>
        <property type="gene ID" value="NP_2862A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702425"/>
    <dbReference type="KEGG" id="nph:NP_2862A"/>
    <dbReference type="eggNOG" id="arCOG01005">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016838_1_0_2"/>
    <dbReference type="OrthoDB" id="50488at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
        <property type="term" value="P:metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1980">
        <property type="entry name" value="Nitrogenase molybdenum iron protein domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050492">
        <property type="entry name" value="Bact_metal-bind_prot9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006127">
        <property type="entry name" value="ZnuA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42953:SF3">
        <property type="entry name" value="HIGH-AFFINITY ZINC UPTAKE SYSTEM PROTEIN ZNUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42953">
        <property type="entry name" value="HIGH-AFFINITY ZINC UPTAKE SYSTEM PROTEIN ZNUA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01297">
        <property type="entry name" value="ZnuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53807">
        <property type="entry name" value="Helical backbone' metal receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="310"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI49522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="463" mass="51102" checksum="84EC8F6339E7DA58" modified="2017-05-10" version="1">MNLSRRGLLTAGAGAATAAVAGCVDAAAPGQESDGGYAAFFTLWDWAEEISGDTLDFENPVSAGQMGHGWSPDGDITREIASSEMFLYLDTPEFSWAQDVATELERDHDEVAVVDALEGLEPYLIPFDTEPMPEPDHGRDYPPESLWFEEVDIYDLRSNQQLGYWHGPGFNHWHGGLPDIAVGDSVPIGVVLKDEAENIVPLGDGESYRVDARLADGEPEGVVDIESRGDRVVFHGESTGSAGIVFQILHEGEVIYETDPEPTSIRVQETLDAGGADEFHDPHAWTDPVLSQRMVDNVADALAEINPESEDTYRDNAEQYKQRLQSVHEELETLVETAERDIAVFAGHDSFQYVEQRYDFELHTPVGISPDAGESFDDMTRLNNVIEEHGIDTVLYDPFESASPGEDLPQMVENIFEETSVDSAEPLSPLEGTTETWQENDWGWVEQMENVNIPSLRKALGAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWT4</accession>
    <name>A0A1U7EWT4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">HD family hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_2902A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323167.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWT4"/>
    <dbReference type="STRING" id="348780.NP_2902A"/>
    <dbReference type="EnsemblBacteria" id="CAI49542">
        <property type="protein sequence ID" value="CAI49542"/>
        <property type="gene ID" value="NP_2902A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701590"/>
    <dbReference type="KEGG" id="nph:NP_2902A"/>
    <dbReference type="eggNOG" id="arCOG04230">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068640_1_0_2"/>
    <dbReference type="OrthoDB" id="103508at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039967">
        <property type="entry name" value="MJ1020-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40517">
        <property type="entry name" value="METAL-DEPENDENT PHOSPHOHYDROLASE, HD SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40517:SF1">
        <property type="entry name" value="METAL-DEPENDENT PHOSPHOHYDROLASE, HD SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD/PDEase" evidence="2">
        <location>
            <begin position="57"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="272" mass="30127" checksum="2DC90C3FFD49ACB0" modified="2017-05-10" version="1">MDEAADSCATGRTYNPEADHAFPDERVNEVLDRIENDEEITAYLAAQNVNPVDRMGYNDHGRKHIEIVRNAALSLYDLLKAGGVEFDGARQQGLDEADEAVIIALAATLHDIGHVVHRDDHPYYSLPLAADILDRFLDGLGYYDPAETVRLKGEILHAILCHHSEEQPLTREAGIVRVADALDMERGRSRIPYEQGGRGINTISSRAITSVALCPGESTPAMVEIEMTDAAGVYQVDELLKHKLDDSRIESFVRIVAVNTDRGEDLIERIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWU0</accession>
    <name>A0A1U7EWU0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glutamine amidotransferase (Homolog to GMP synthase subunit A)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">guaAa4</name>
        <name evidence="2" type="ordered locus">NP_2928A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323179.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWU0"/>
    <dbReference type="STRING" id="348780.NP_2928A"/>
    <dbReference type="EnsemblBacteria" id="CAI49555">
        <property type="protein sequence ID" value="CAI49555"/>
        <property type="gene ID" value="NP_2928A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702782"/>
    <dbReference type="KEGG" id="nph:NP_2928A"/>
    <dbReference type="eggNOG" id="arCOG00090">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1222558_0_0_2"/>
    <dbReference type="OrthoDB" id="3321at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01741">
        <property type="entry name" value="GATase1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044992">
        <property type="entry name" value="ChyE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42695">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE YLR126C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42695:SF5">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE YLR126C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="1">
        <location>
            <begin position="48"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="226" mass="24262" checksum="572E594169CFA75F" modified="2017-05-10" version="1">MRTKFDGKEIYVVKSEPDEERTYHCDALASRFPTATEIDYPAGERVPVDSAAAVVLTGSTAAVYEPEQYPWVDDQQALVRRLVERSVPTLGVCFGHQIANAALGGTVEHVGMTAGLVEADLDHQPLFEGVAPVVPALHGDAVTALGDGMESIATAPHADIFASRHQSAPLWTVQFHPEITAALEPRLTEDFGWESGRWSFGDVTAAVVFENFKSMAESAPAAADSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWV4</accession>
    <name>A0A1U7EWV4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2946A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323188.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWV4"/>
    <dbReference type="EnsemblBacteria" id="CAI49564">
        <property type="protein sequence ID" value="CAI49564"/>
        <property type="gene ID" value="NP_2946A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701607"/>
    <dbReference type="KEGG" id="nph:NP_2946A"/>
    <dbReference type="eggNOG" id="arCOG04617">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126924_0_0_2"/>
    <dbReference type="OrthoDB" id="213677at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="183" mass="20320" checksum="CC4CE14EB0DE81CF" modified="2017-05-10" version="1">MRVRDWQEILEDVVESDAEPSGWRAVAGDRRGGVGEDMFLGHPSVGVFQLKTFAKNPYEVQGVGSRVARKVDDELDPLFPGEESGGRFGVNQAFEDTDEATERAKELETVIETHAEAPTTGDALFEDVMGALDSPAFGPMEYDMYDRPDELDDLTDTFEEAEEVLSKELDDLIEDDNVGRGFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EWV7</accession>
    <name>A0A1U7EWV7_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TFIIB-type zinc ribbon-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_2996A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323213.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EWV7"/>
    <dbReference type="STRING" id="348780.NP_2996A"/>
    <dbReference type="EnsemblBacteria" id="CAI49589">
        <property type="protein sequence ID" value="CAI49589"/>
        <property type="gene ID" value="NP_2996A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701620"/>
    <dbReference type="KEGG" id="nph:NP_2996A"/>
    <dbReference type="eggNOG" id="arCOG04625">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077072_0_0_2"/>
    <dbReference type="OrthoDB" id="341613at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055541">
        <property type="entry name" value="DUF7117"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23430">
        <property type="entry name" value="DUF7117"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="242" mass="26919" checksum="23DF7DB39193132D" modified="2017-05-10" version="1">MKVRGRRECKACGTRWSYYDTGDTACPSCGSLHSVGTDDERKLHTATAATLDLTPVREAVDDVPLRRLATRAIERTREFTRGYGFIDGGSLQPLDETYLAAMELRYVAGELERRFDPTDEETLYLTTLFKADKGVRPDVGDVPSELRAMRGLAYADAVDAYRSDLKAYLDDQPDPVVGGHVERLADRVRRIQALDGDVPSRQAESLVAVARDIGRYIADGDENALARADERLDTDPTRAEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX21</accession>
    <name>A0A1U7EX21_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tryptophan synthase beta chain</fullName>
            <ecNumber evidence="12">4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">trpB1</name>
        <name evidence="12" type="synonym">trpB</name>
        <name evidence="14" type="ordered locus">NP_3164A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 12">The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + L-tryptophan + H2O</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 12">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the TrpB family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="12"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323295.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX21"/>
    <dbReference type="STRING" id="348780.NP_3164A"/>
    <dbReference type="EnsemblBacteria" id="CAI49673">
        <property type="protein sequence ID" value="CAI49673"/>
        <property type="gene ID" value="NP_3164A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703408"/>
    <dbReference type="KEGG" id="nph:NP_3164A"/>
    <dbReference type="eggNOG" id="arCOG01433">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016734_3_1_2"/>
    <dbReference type="OrthoDB" id="371827at2157"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00044"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06446">
        <property type="entry name" value="Trp-synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000004">
        <property type="entry name" value="Tryptophan synthase beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00133">
        <property type="entry name" value="Trp_synth_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006653">
        <property type="entry name" value="Trp_synth_b_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006654">
        <property type="entry name" value="Trp_synth_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023026">
        <property type="entry name" value="Trp_synth_beta/beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00263">
        <property type="entry name" value="trpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48077:SF3">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48077">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001413">
        <property type="entry name" value="Trp_syn_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00168">
        <property type="entry name" value="TRP_SYNTHASE_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 12" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="10 12 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 12" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="13">
        <location>
            <begin position="54"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="12">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAI49673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="417" mass="45224" checksum="F3043C37739EB122" modified="2017-05-10" version="1">MSEPDTKFGDYGGQYVPEALMPAIEELTDAYERYVLENEDGFMDEFRERLRDFGGRPTPLQRADQLSERYDHEIYLKREDLLHGGAHKLNNALGQVLLAKYMGKERIIAETGAGQHGTATAMAAAHLDMPCEIYMGRRDINRQRPNVFRMRINGAAVNPVDIGRGTLKEAISETMRDWATTVERTHYVIGSIVGPAPFPAMVRDFHSVISEEIRQQSRDQLGELPGSVVACAGGGSNTMGAFAEFVADDVDLYAVEAGGSSLTVDEEEGVAPHSASLSTGEEGVLHGARTKLLQDSHGQIMESHSVSSGLDYSGVGPELSHLVDEGRVTPVTVGDEPALEAFHRLSQAEGIIPALETAHAFGFLEETLGPDGADEAAIEEPVVVNVSGRGDKDLEAAIEETEKRSLDAAPTMEVFEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX35</accession>
    <name>A0A1U7EX35_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ferredoxin (4Fe-4S)</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3176A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323301.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX35"/>
    <dbReference type="EnsemblBacteria" id="CAI49679">
        <property type="protein sequence ID" value="CAI49679"/>
        <property type="gene ID" value="NP_3176A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701677"/>
    <dbReference type="KEGG" id="nph:NP_3176A"/>
    <dbReference type="eggNOG" id="arCOG04765">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2127757_0_0_2"/>
    <dbReference type="OrthoDB" id="223840at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="111" mass="12043" checksum="8047206E6DD4ABCE" modified="2017-05-10" version="1">MPDEPVDPSDIGEGDAPPVEAAPYKIIFEANKCIAAGKCAEVSANWEMDIDTGIAKPDVYFFDESDLEHNIEAAEACPAKKDRGVIHVVDRRTDEELAPDPKGDGTLSVDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EX77</accession>
    <name>A0A1U7EX77_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3362A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323392.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EX77"/>
    <dbReference type="STRING" id="348780.NP_3362A"/>
    <dbReference type="EnsemblBacteria" id="CAI49772">
        <property type="protein sequence ID" value="CAI49772"/>
        <property type="gene ID" value="NP_3362A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701749"/>
    <dbReference type="KEGG" id="nph:NP_3362A"/>
    <dbReference type="eggNOG" id="arCOG01140">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129109_0_0_2"/>
    <dbReference type="OrthoDB" id="210127at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058877">
        <property type="entry name" value="JAB/MPN_dom-containing"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26422">
        <property type="entry name" value="Halo_JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="159" mass="16536" checksum="1994442089BACDF6" modified="2017-05-10" version="1">MVYITRGLLAALCELAAERDPRSVTVALTVTPASELAPAGPAADDGSATVPARRNDVDLSALQPDADVFTDMYLPDTGGSVTAVFGMDLSTPGSAGRFISHPEGPFGLTKADDLHEVVFIAVPPYDRDAVAAFDRRGNVLTVTVVKAVPPEGSVEPLDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXB4</accession>
    <name>A0A1U7EXB4_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3412A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323417.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXB4"/>
    <dbReference type="STRING" id="348780.NP_3412A"/>
    <dbReference type="EnsemblBacteria" id="CAI49797">
        <property type="protein sequence ID" value="CAI49797"/>
        <property type="gene ID" value="NP_3412A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701770"/>
    <dbReference type="KEGG" id="nph:NP_3412A"/>
    <dbReference type="eggNOG" id="arCOG06385">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115705_0_0_2"/>
    <dbReference type="OrthoDB" id="258012at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="162" mass="18178" checksum="267FEA48B0A95CFD" modified="2017-05-10" version="1">MVREYITSREYLPDDPEGEHRLQLTDAETKEIFETQVRIAKSKAELADPAPLVVRGGPHEDSHEAWYVELVESDAGGEIDRELLRDCLEDAQSGTDVINARSAELKAVLCYLVEKGVFESQSEAARSLLWEKLADDHRPLLRQLDGVCDELEESDLDAALGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXC0</accession>
    <name>A0A1U7EXC0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3442A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49812.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323432.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXC0"/>
    <dbReference type="STRING" id="348780.NP_3442A"/>
    <dbReference type="EnsemblBacteria" id="CAI49812">
        <property type="protein sequence ID" value="CAI49812"/>
        <property type="gene ID" value="NP_3442A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701774"/>
    <dbReference type="KEGG" id="nph:NP_3442A"/>
    <dbReference type="eggNOG" id="arCOG04630">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1754671_0_0_2"/>
    <dbReference type="OrthoDB" id="202378at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043858">
        <property type="entry name" value="DUF5820"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19137">
        <property type="entry name" value="DUF5820"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49812.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="142" mass="15869" checksum="93B8BE1C898847C0" modified="2017-05-10" version="1">MKRSAVSDDSTPDPSTLGDGWDIWSADSDRTVLAYRPDVFDGDEFPPPCLPTVYITRGRPNRRPEGNRNLPPDAPWMVTLYLEPEVNRDAEAYDTAAEAAAAAARLTRRFADGDVDYRGLYQIPRESYFERLDELTGGPTAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXC9</accession>
    <name>A0A1U7EXC9_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Enoyl-CoA hydratase</fullName>
            <ecNumber evidence="4">4.2.1.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">fadA4</name>
        <name evidence="4" type="ordered locus">NP_3434A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.17" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323428.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXC9"/>
    <dbReference type="STRING" id="348780.NP_3434A"/>
    <dbReference type="DNASU" id="3702681"/>
    <dbReference type="EnsemblBacteria" id="CAI49808">
        <property type="protein sequence ID" value="CAI49808"/>
        <property type="gene ID" value="NP_3434A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702681"/>
    <dbReference type="KEGG" id="nph:NP_3434A"/>
    <dbReference type="eggNOG" id="arCOG00239">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_6_2"/>
    <dbReference type="OrthoDB" id="27846at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000009">
        <property type="entry name" value="Carnitinyl-CoA dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.12.10:FF:000001">
        <property type="entry name" value="Probable enoyl-CoA hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941:SF54">
        <property type="entry name" value="ENOYL-COA HYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941">
        <property type="entry name" value="ENOYL-COA HYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI49808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="254" mass="27011" checksum="5DC5201871D2FC20" modified="2017-05-10" version="1">MEWETVSLEWNDSIATVTVDRPEALNALNVETLEALAEAIAAAEAGDARVLVLAGAGDDAFIAGADIKYMKDLSTPEAQAWGELGHDVADALEQFPGATVAAVDGYAFGGGCEMAMACDFRIASESALIGNTEIDLGIIPGWGGTQRLPRLVGEETAKRMIFLGERLDAETAAAEGLFGEVVSDDEVDNRIEEFAAELASKPAVALKAAKQAIEQRHGTGGLDYEKRAFAGLFGTHDQREGMKAFVEDRTPEFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXI5</accession>
    <name>A0A1U7EXI5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_3630A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323526.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXI5"/>
    <dbReference type="EnsemblBacteria" id="CAI49906">
        <property type="protein sequence ID" value="CAI49906"/>
        <property type="gene ID" value="NP_3630A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701830"/>
    <dbReference type="KEGG" id="nph:NP_3630A"/>
    <dbReference type="eggNOG" id="arCOG05251">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_803187_0_0_2"/>
    <dbReference type="OrthoDB" id="326898at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI49906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="318" mass="34489" checksum="2650E738BDC66CF0" modified="2017-05-10" version="1">MERHRVSPADLFSLGVVSELETAIEAVAAGRNVAVVAPPLAGRAAVLDIVEDMVGESVREQATERTDTADAPIRLYEDCHRLYLRHIGGFSELDAFLDGMAETSAVHVTTWNAYAWAYLNQAKSVGNSFDSVITLDKPDSETVTSWLESLAADGLTYVRDSADGPSGFDPQKIHSLTTLREQLRLLCFADGDDDDPKTAVFDSVTRITGGNPGVAKTIWNAIEAEAATEGEVRTSMVTEHEAEVSELSYDDAYALLAVLANGSITQSELEAVVDESLDRTLRRFEKQGVVELTGDGISVAPPALKPVVEHLTNRRLLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXP6</accession>
    <name>A0A1U7EXP6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_3744A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323580.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXP6"/>
    <dbReference type="STRING" id="348780.NP_3744A"/>
    <dbReference type="EnsemblBacteria" id="CAI49963">
        <property type="protein sequence ID" value="CAI49963"/>
        <property type="gene ID" value="NP_3744A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701853"/>
    <dbReference type="KEGG" id="nph:NP_3744A"/>
    <dbReference type="HOGENOM" id="CLU_2127910_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI49963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="113" mass="11640" checksum="6C939C94A2C5A830" modified="2017-05-10" version="1">MVRLVAAARSPWAYIGLWLPVVTVVAFLLADFVGVVAVPSAVLAVTLAAALGWVVVGVGGSRTDPEFALRARGEQQPAAKANGRDSDRPLGTAVLVYLTATTGLGWLAVVTVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXU5</accession>
    <name>A0A1U7EXU5_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">site-specific DNA-methyltransferase (adenine-specific)</fullName>
            <ecNumber evidence="1">2.1.1.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">NP_3874A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N(6)-methyl-2'-deoxyadenosine in DNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15197"/>
            <dbReference type="Rhea" id="RHEA-COMP:12418"/>
            <dbReference type="Rhea" id="RHEA-COMP:12419"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90615"/>
            <dbReference type="ChEBI" id="CHEBI:90616"/>
            <dbReference type="EC" id="2.1.1.72"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.72" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323645.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="STRING" id="348780.NP_3874A"/>
    <dbReference type="EnsemblBacteria" id="CAI50028">
        <property type="protein sequence ID" value="CAI50028"/>
        <property type="gene ID" value="NP_3874A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701897"/>
    <dbReference type="KEGG" id="nph:NP_3874A"/>
    <dbReference type="eggNOG" id="arCOG04814">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002881_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009007">
        <property type="term" value="F:site-specific DNA-methyltransferase (adenine-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006304">
        <property type="term" value="P:DNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011639">
        <property type="entry name" value="MethylTrfase_TaqI-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050953">
        <property type="entry name" value="N4_N6_ade-DNA_methylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33841:SF1">
        <property type="entry name" value="DNA METHYLTRANSFERASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33841">
        <property type="entry name" value="DNA METHYLTRANSFERASE YEEA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07669">
        <property type="entry name" value="Eco57I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00507">
        <property type="entry name" value="N12N6MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Type II methyltransferase M.TaqI-like" evidence="7">
        <location>
            <begin position="584"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="655"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="655"/>
            <end position="672"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAI50028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="1342" mass="153725" checksum="BA2BDA945196474D" modified="2017-05-10" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXX1</accession>
    <name>A0A1U7EXX1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC-type transport system ATP-binding protein (Probable substrate copper)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">nosF1</name>
        <name evidence="5" type="synonym">abc11a</name>
        <name evidence="5" type="ordered locus">NP_3918A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323667.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXX1"/>
    <dbReference type="STRING" id="348780.NP_3918A"/>
    <dbReference type="EnsemblBacteria" id="CAI50050">
        <property type="protein sequence ID" value="CAI50050"/>
        <property type="gene ID" value="NP_3918A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702402"/>
    <dbReference type="KEGG" id="nph:NP_3918A"/>
    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OrthoDB" id="40048at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03230">
        <property type="entry name" value="ABC_DR_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051782">
        <property type="entry name" value="ABC_Transporter_VariousFunc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42939">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN ALBC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42939:SF1">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN ALBC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="14"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAI50050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="248" mass="25294" checksum="D26C3453359465D8" modified="2017-05-10" version="1">MSDGPETPTDGPVLTADGVSKSFGPVDVLSDISCVIPAEEVVAIIGPNGSGKTTLLRTLVGDLRPTAGEVTYHGPSVDRPIGYLPQRATFRSGFSCAETLSFYGSFVDGPDPATLLERVGLEGAADRNVEALSGGMRRLLGIAQAMVGDPPLVVLDEPASGLDPNMSASVFETAGEMAGGGTTVLLSSHDMSLVEAVADTVLVVSQGDVVASGAVAELYDRFEVSSLRGVIGSVVDDSDRVAVLGGDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EXY5</accession>
    <name>A0A1U7EXY5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Replication protein A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpa1</name>
        <name evidence="3" type="ordered locus">NP_3960A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323687.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EXY5"/>
    <dbReference type="STRING" id="348780.NP_3960A"/>
    <dbReference type="EnsemblBacteria" id="CAI50071">
        <property type="protein sequence ID" value="CAI50071"/>
        <property type="gene ID" value="NP_3960A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701922"/>
    <dbReference type="KEGG" id="nph:NP_3960A"/>
    <dbReference type="eggNOG" id="arCOG01510">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031172_1_0_2"/>
    <dbReference type="OrthoDB" id="335252at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010212">
        <property type="term" value="P:response to ionizing radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04491">
        <property type="entry name" value="SoSSB_OBF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051231">
        <property type="entry name" value="SOSS-B"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005554">
        <property type="entry name" value="PRK07218.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13356">
        <property type="entry name" value="OB FOLD NUCLEIC ACID BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13356:SF10">
        <property type="entry name" value="REPLICATION FACTOR-A PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="39"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="425" mass="45848" checksum="67D3B13D54A0E5EE" modified="2017-05-10" version="1">MSLDDAAEDLASSLGVDKAEVKEDLEKLVSYSVPMDEAKQSLRRKYGDEGSTGGGADDAPTTDIEEITTDDDRVTVTARVLTVGKRSIRYDGDDQTIFEGELADGTGRISYTAWTDFSLTPGDTVTVGNAGVREWDGNPELNLGQSTTVTLEEEPLSVPYDVGGQRNLIDLEPGDRGRTVEVRVLEVEQKTIDGRDGETEILDGVFGDETSRLPFTDWDPHDAIEAGTSVRLEDVYVDEFRGVPSVNVTAFTTVEAIPEVAVSDEATAMSVQEAVDAGGVYDVELTGNIIEVRDGSGLIQRCPECGRVVQNDQCRSHGKVDPEDDLRVKAILDDGTDSVTVILGRELTEEVYGGTLTDALEAARDEMDRGVVADDIAETLAGREYRVRGSLSVDEYGANLDATDFEESGGDPASRAERFLAEVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY59</accession>
    <name>A0A1U7EY59_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Homolog to NAD kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4096A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323755.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY59"/>
    <dbReference type="STRING" id="348780.NP_4096A"/>
    <dbReference type="EnsemblBacteria" id="CAI50139">
        <property type="protein sequence ID" value="CAI50139"/>
        <property type="gene ID" value="NP_4096A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701970"/>
    <dbReference type="KEGG" id="nph:NP_4096A"/>
    <dbReference type="eggNOG" id="arCOG01350">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064691_0_0_2"/>
    <dbReference type="OrthoDB" id="56451at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039065">
        <property type="entry name" value="AcoX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011386">
        <property type="entry name" value="Put_ATP-NAD_kin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40697">
        <property type="entry name" value="ACETOIN CATABOLISM PROTEIN X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40697:SF2">
        <property type="entry name" value="ATP-NAD KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20143">
        <property type="entry name" value="NAD_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016907">
        <property type="entry name" value="Kin_ATP-NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="355" mass="37544" checksum="26A160053B97BA94" modified="2017-05-10" version="1">MRRIGLVVNPIAGMGGRVGLKGTDNKVEEARRRGAEPRAPDRARQALEHLEEQSTEVAVYTYGGVMGEDEAVAAGFEPTVVGRPDGDETSTADTRAAVRALVDADVDLILFVGGDGTAVDVAETLDELDDATPILGVPGGVKVYSSVFAVTPRAAGRIATTFERTERREVNDIDEDAYRGGDVNTELKALAEVPVGDEIQSSKQIGGGTVEALAEAVAAEVDPETTYVLGPGSTVDAVKTELGFNGSPLGVDVWRGGEVVARDAGESDILDALGERNVIVVSPIGGQGFIFGRGNDQISPPVIRQSDIEVVASKQKLKDIGVLRVDTGDPELDEALRGWMRVRVGKFERQLLKVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY82</accession>
    <name>A0A1U7EY82_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4160A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323787.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY82"/>
    <dbReference type="STRING" id="348780.NP_4160A"/>
    <dbReference type="EnsemblBacteria" id="CAI50171">
        <property type="protein sequence ID" value="CAI50171"/>
        <property type="gene ID" value="NP_4160A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701989"/>
    <dbReference type="KEGG" id="nph:NP_4160A"/>
    <dbReference type="eggNOG" id="arCOG02846">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_989069_0_0_2"/>
    <dbReference type="OrthoDB" id="262791at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="271" mass="29187" checksum="3BE898303F8F8C5C" modified="2017-05-10" version="1">MQWRCEWCGRNYESDEPPATCETCGRESFEADTDDDPFESEQIVWACADCGREHVKHSPPCARCGGHNLERQRVTVDVDEDVSAPGYLSVGAPYLLGALVVVVIVGLALVGVIPVPGLSGPPAPPDAPADEERTASLSLAEVEANLHDRFETERDSEGVQSRTLDGGDTGAFVTYLTRHTVAERYDDGYDGDGPDPSAFDIRCQRQPAVGVFETAVDADAFNSESALADELATAMLDVTEYRSAIRNDAQREGISVHVAPDGTVFVGYLSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY91</accession>
    <name>A0A1U7EY91_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">fructose-bisphosphatase</fullName>
            <ecNumber evidence="2">3.1.3.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">suhB</name>
        <name evidence="9" type="ordered locus">NP_4188A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="EC" id="3.1.3.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the inositol monophosphatase superfamily. FBPase class 4 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.11" evidence="2"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323801.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY91"/>
    <dbReference type="STRING" id="348780.NP_4188A"/>
    <dbReference type="EnsemblBacteria" id="CAI50185">
        <property type="protein sequence ID" value="CAI50185"/>
        <property type="gene ID" value="NP_4188A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703222"/>
    <dbReference type="KEGG" id="nph:NP_4188A"/>
    <dbReference type="eggNOG" id="arCOG01349">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044118_0_2_2"/>
    <dbReference type="OrthoDB" id="58111at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042132">
        <property type="term" value="F:fructose 1,6-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008934">
        <property type="term" value="F:inositol monophosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006020">
        <property type="term" value="P:inositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01637">
        <property type="entry name" value="IMPase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020583">
        <property type="entry name" value="Inositol_monoP_metal-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20854">
        <property type="entry name" value="INOSITOL MONOPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20854:SF4">
        <property type="entry name" value="INOSITOL-1-MONOPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00629">
        <property type="entry name" value="IMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR600760-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAI50185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="267" mass="28318" checksum="BB33F71E4638786F" modified="2017-05-10" version="1">MDERADCARRAAEAGADIAAAAFRDGIDSETKGEKTDVVTQADRDAQRRVVGVIRERYPDEPIVGEESDGETEATADELPAEGAAWVVDPIDGTNNFVHGLREWATSVVAVVDGAAVAGATVAPALGETFLLTPDGAFLNGDPLAVSDRSDPETFTVVPTVWWGFDRRDEYAATCEAIVQQFGDMRRFGSAQLELGYCAAGAVEAVVTNIDPEPWDTVLGAALVRAAGGTVTDIHGEPWRHDSTGLVASNGRAHDAVLEAARKPERQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EY93</accession>
    <name>A0A1U7EY93_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Nitrite reductase (Ferredoxin)</fullName>
            <ecNumber evidence="10">1.7.7.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">nasD</name>
        <name evidence="10" type="synonym">nirA1</name>
        <name evidence="10" type="ordered locus">NP_4224A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.</text>
    </comment>
    <dbReference type="EC" id="1.7.7.1" evidence="10"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323819.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EY93"/>
    <dbReference type="STRING" id="348780.NP_4224A"/>
    <dbReference type="EnsemblBacteria" id="CAI50203">
        <property type="protein sequence ID" value="CAI50203"/>
        <property type="gene ID" value="NP_4224A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702975"/>
    <dbReference type="KEGG" id="nph:NP_4224A"/>
    <dbReference type="eggNOG" id="arCOG02055">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015667_2_3_2"/>
    <dbReference type="OrthoDB" id="15347at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048307">
        <property type="term" value="F:ferredoxin-nitrite reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.480.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.413.10">
        <property type="entry name" value="Sulfite Reductase Hemoprotein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051329">
        <property type="entry name" value="NIR_SIR_4Fe-4S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005117">
        <property type="entry name" value="NiRdtase/SiRdtase_haem-b_fer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036136">
        <property type="entry name" value="Nit/Sulf_reduc_fer-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006067">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045854">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006066">
        <property type="entry name" value="NO2/SO3_Rdtase_FeS/sirohaem_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32439">
        <property type="entry name" value="FERREDOXIN--NITRITE REDUCTASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32439:SF0">
        <property type="entry name" value="FERREDOXIN--NITRITE REDUCTASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01077">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03460">
        <property type="entry name" value="NIR_SIR_ferr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00397">
        <property type="entry name" value="SIROHAEM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56014">
        <property type="entry name" value="Nitrite and sulphite reductase 4Fe-4S domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55124">
        <property type="entry name" value="Nitrite/Sulfite reductase N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00365">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="1"/>
    </dbReference>
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</entry>
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        <name evidence="1" type="ordered locus">NP_4852A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324129.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZ41"/>
    <dbReference type="STRING" id="348780.NP_4852A"/>
    <dbReference type="EnsemblBacteria" id="CAI50517">
        <property type="protein sequence ID" value="CAI50517"/>
        <property type="gene ID" value="NP_4852A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702197"/>
    <dbReference type="KEGG" id="nph:NP_4852A"/>
    <dbReference type="eggNOG" id="arCOG04069">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017233_1_0_2"/>
    <dbReference type="OrthoDB" id="4144at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd18086">
        <property type="entry name" value="HsC9orf114-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003750">
        <property type="entry name" value="Put_MeTrfase-C9orf114-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12150">
        <property type="entry name" value="CLASS IV SAM-BINDING METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12150:SF13">
        <property type="entry name" value="METHYLTRANSFERASE C9ORF114-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02598">
        <property type="entry name" value="Methyltrn_RNA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="278" mass="30041" checksum="0D450DE5416DC92B" modified="2017-05-10" version="1">MTRSVLVPSSLTREAEDKREATRKLGYVARAATVFRVDRLVVYPDRDGDDGRFGDGFVSTVLEYAATPAYLRKEVWDRRGELEYAGILPPLRVRSQTGSGSDGSGSLRQGIVTEVGPDGRVRVNCGMQHPISLPVPPSMEVGKGERVTIRVSSREPVRAKLVDESPPGFAVDCATIEASLARPDAGVRIAASRHGETLSVDRLDGIVGRTTANGFTVAFGAPERGLPEILGCEPREEAIGDEPEARFDLWLNTVPNQGSEVVRTEEALFATLAPLTLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZ56</accession>
    <name>A0A1U7EZ56_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Thioesterase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_4924A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
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    <dbReference type="EnsemblBacteria" id="CAI50553">
        <property type="protein sequence ID" value="CAI50553"/>
        <property type="gene ID" value="NP_4924A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702489"/>
    <dbReference type="KEGG" id="nph:NP_4924A"/>
    <dbReference type="eggNOG" id="arCOG00773">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050164_3_2_2"/>
    <dbReference type="OrthoDB" id="15030at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052816">
        <property type="term" value="F:long-chain fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03442">
        <property type="entry name" value="BFIT_BACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040170">
        <property type="entry name" value="Cytosol_ACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033120">
        <property type="entry name" value="HOTDOG_ACOT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11049">
        <property type="entry name" value="ACYL COENZYME A THIOESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11049:SF24">
        <property type="entry name" value="CYTOSOLIC ACYL COENZYME A THIOESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51770">
        <property type="entry name" value="HOTDOG_ACOT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HotDog ACOT-type" evidence="2">
        <location>
            <begin position="5"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="142" mass="15895" checksum="55BC1EBE48C2D27E" modified="2017-05-10" version="1">MVELLETFIEDRNRIQPHHANNLGTAHGGNVLNWMDEAGAMSAMRFAGSPCVTAHINSVDFERPLEIGEIALVEAYAYEAGRTSVRVRVRAYGEDPLNGDREVTTESYFVFVAVNEDLRPVEVPELDVETEEGKRLQEMADE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZI6</accession>
    <name>A0A1U7EZI6_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5238A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZI6"/>
    <dbReference type="STRING" id="348780.NP_5238A"/>
    <dbReference type="EnsemblBacteria" id="CAI50710">
        <property type="protein sequence ID" value="CAI50710"/>
        <property type="gene ID" value="NP_5238A"/>
    </dbReference>
    <dbReference type="GeneID" id="54763332"/>
    <dbReference type="KEGG" id="nph:NP_5238A"/>
    <dbReference type="eggNOG" id="arCOG14540">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3194603_0_0_2"/>
    <dbReference type="OrthoDB" id="226402at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="46" mass="5131" checksum="BDFD3A9D706A5222" modified="2017-05-10" version="1">MTNENVDPMVPVMPTEETTPDVRADGGRRKRYDNAEVDAPLVPDCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZI8</accession>
    <name>A0A1U7EZI8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DUF2103 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">NP_5230A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007426.1"/>
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    <dbReference type="EnsemblBacteria" id="CAI50706">
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        <property type="gene ID" value="NP_5230A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702305"/>
    <dbReference type="KEGG" id="nph:NP_5230A"/>
    <dbReference type="eggNOG" id="arCOG02192">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1154327_0_0_2"/>
    <dbReference type="OrthoDB" id="50478at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018664">
        <property type="entry name" value="DUF2103_metal-binding"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09876">
        <property type="entry name" value="DUF2103"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAI50706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="240" mass="26276" checksum="ED07FB106D2BC0F4" modified="2017-05-10" version="1">MECRCCASPLERPGDYCLVCRSANADTVVIDCERTRATVSSLRDEDVVAEREITTTPVDDERWAPTERRNFAGRIADEVQRKRPEEVYAAGERSVVRAVRAELHYPFYRIADDDPIAAVKRRRGDPALEVVEASVEEKLGGSHSTLIGGRDGRLALETVAGHPHVKKIIPGPIDAGGSGSRTGVRAKATRADEHGNVRLLIRDGSSVQENRVVTTAGSRELGEHVRGDLNEALSETELQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZK5</accession>
    <name>A0A1U7EZK5_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DUF2062 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_5288A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324344.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZK5"/>
    <dbReference type="STRING" id="348780.NP_5288A"/>
    <dbReference type="EnsemblBacteria" id="CAI50735">
        <property type="protein sequence ID" value="CAI50735"/>
        <property type="gene ID" value="NP_5288A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702321"/>
    <dbReference type="KEGG" id="nph:NP_5288A"/>
    <dbReference type="eggNOG" id="arCOG06355">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1590937_0_0_2"/>
    <dbReference type="OrthoDB" id="329979at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018639">
        <property type="entry name" value="DUF2062"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40547">
        <property type="entry name" value="SLL0298 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40547:SF1">
        <property type="entry name" value="SLL0298 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09835">
        <property type="entry name" value="DUF2062"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2062" evidence="2">
        <location>
            <begin position="16"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="167" mass="17597" checksum="D6A876D8F7895258" modified="2017-05-10" version="1">MALLRARLGETVDDLRGRLHAALAERYTPGQVAGSFAVGVFITSLPTLGTGLLLFPPLSYLFAGISKVALVAAVAVMNPVVKWGVYGVSFWLGSRLLGPVEGVARGDIGLSAAPEVVTRLLVGNLLLAVVFTVVGYAVAYRLTVEYRQQNGGVGRIEGALEAVFGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZQ0</accession>
    <name>A0A1U7EZQ0_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_5366A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324382.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZQ0"/>
    <dbReference type="STRING" id="348780.NP_5366A"/>
    <dbReference type="EnsemblBacteria" id="CAI50774">
        <property type="protein sequence ID" value="CAI50774"/>
        <property type="gene ID" value="NP_5366A"/>
    </dbReference>
    <dbReference type="GeneID" id="54763336"/>
    <dbReference type="KEGG" id="nph:NP_5366A"/>
    <dbReference type="eggNOG" id="arCOG07540">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2230455_0_0_2"/>
    <dbReference type="OrthoDB" id="275832at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAI50774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="105" mass="11271" checksum="C8F6EFC0D2E8AD59" modified="2017-05-10" version="1">MSTGTTTDPCGSDRLDESAADNDTDDRCVCGAPADGYGALRDGPVCADCASRATPRWRLQLSFGDLTLRSEWTSEWAVVENLADQLRCDDAASEVVIERLAEVDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZQ8</accession>
    <name>A0A1U7EZQ8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NP_3111F</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CDN71456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZQ8"/>
    <dbReference type="EnsemblBacteria" id="CDN71456">
        <property type="protein sequence ID" value="CDN71456"/>
        <property type="gene ID" value="NP_3111F"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_3111F"/>
    <dbReference type="eggNOG" id="arCOG14360">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2985780_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CDN71456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="57" mass="6389" checksum="D6BF6986B6A6659B" modified="2017-05-10" version="1">MEPPKALMVVDLRELALIYEMSKEASQDTARHSSDRGTAAELHEKCTELLGRDETSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-05-10" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1U7EZR7</accession>
    <name>A0A1U7EZR7_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Small CPxCG-related zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">NP_4711H</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CCI69586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1U7EZR7"/>
    <dbReference type="STRING" id="348780.NP_4711H"/>
    <dbReference type="EnsemblBacteria" id="CCI69586">
        <property type="protein sequence ID" value="CCI69586"/>
        <property type="gene ID" value="NP_4711H"/>
    </dbReference>
    <dbReference type="KEGG" id="nph:NP_4711H"/>
    <dbReference type="eggNOG" id="arCOG07555">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2447755_0_0_2"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058419">
        <property type="entry name" value="DUF8106"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26408">
        <property type="entry name" value="DUF8106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8106" evidence="1">
        <location>
            <begin position="26"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCI69586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="89" mass="9777" checksum="46B7513CBEE6EF9D" modified="2017-05-10" version="1">MDWRYTRYIVSTNNSANGAAHNSYTQKGKLFCTSCSYSSRYDGGWTTVEADGTLHYLCPCCGTEITTRTADTTPHSQTARVPAVGGEKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILU1</accession>
    <name>Q3ILU1_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">NP_3302A</name>
        <name evidence="4" type="ordered locus">NP_7026A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4 5">
        <name>PL23</name>
    </geneLocation>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
            <strain evidence="4">Gabara</strain>
            <plasmid evidence="4">PL23</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CR936259">
        <property type="protein sequence ID" value="CAI50929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323363.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILU1"/>
    <dbReference type="STRING" id="348780.NP_3302A"/>
    <dbReference type="EnsemblBacteria" id="CAI49742">
        <property type="protein sequence ID" value="CAI49742"/>
        <property type="gene ID" value="NP_3302A"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CAI50929">
        <property type="protein sequence ID" value="CAI50929"/>
        <property type="gene ID" value="NP_7026A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703473"/>
    <dbReference type="KEGG" id="nph:NP_3302A"/>
    <dbReference type="KEGG" id="nph:NP_7026A"/>
    <dbReference type="HOGENOM" id="CLU_851568_0_0_2"/>
    <dbReference type="OrthoDB" id="387127at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="281"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI49742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAI50929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="326" mass="35292" checksum="AB8371758D0381D0" modified="2005-11-08" version="1">MTDDDLPGDDVLDDDLLEDAETEDTDPKTRGKKAGERMRQKTENFVLWTGGVAGGLTSTAFRIVPFTGRLFKRMAVKSIENYHKKAGGDAVGIVAKEGQQIDLIPVKYRTPEEVDEGEKAGWKAKGIDKVWNPGPEGYSVNYIGRTPTVPLQRDDHVEAGWLKPRIGTAIELDNYWPLFVNPDITANIDYGAAGGSQRARADGGINDPAVSFELDSPGEWAGDNIIDLDSGDGYDGMRISFNKAKEWRAETTTSESMQMQEDRGFLRGRLSGDEGPSLFKLLLLCAAIILGVLAIVLIGPQLVGGEDGGSSMNPLLVNSLMAFGAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ILY8</accession>
    <name>Q3ILY8_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">NP_6202A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11 12">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324479.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ILY8"/>
    <dbReference type="EnsemblBacteria" id="CAI50881">
        <property type="protein sequence ID" value="CAI50881"/>
        <property type="gene ID" value="NP_6202A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694640"/>
    <dbReference type="KEGG" id="nph:NP_6202A"/>
    <dbReference type="HOGENOM" id="CLU_000445_114_58_2"/>
    <dbReference type="OrthoDB" id="8127at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00075">
        <property type="entry name" value="HATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00156">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052162">
        <property type="entry name" value="Sensor_kinase/Photoreceptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43304:SF1">
        <property type="entry name" value="PAC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43304">
        <property type="entry name" value="PHYTOCHROME-LIKE PROTEIN CPH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0614">Plasmid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Response regulatory" evidence="8">
        <location>
            <begin position="14"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="9">
        <location>
            <begin position="146"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="10">
        <location>
            <begin position="220"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="9">
        <location>
            <begin position="273"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="10">
        <location>
            <begin position="349"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="9">
        <location>
            <begin position="399"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="10">
        <location>
            <begin position="476"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="538"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="6">
        <location>
            <position position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAI50881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="742" mass="82413" checksum="6C8B3F0B24FACFDD" modified="2005-11-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IM60</accession>
    <name>Q3IM60_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="3">3-hydroxybutyrate dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.30</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">bdhA</name>
        <name evidence="3" type="ordered locus">NP_6040A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>PL131</name>
    </geneLocation>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.30" evidence="3"/>
    <dbReference type="EMBL" id="CR936258">
        <property type="protein sequence ID" value="CAI50800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324407.1">
        <property type="nucleotide sequence ID" value="NC_007427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IM60"/>
    <dbReference type="EnsemblBacteria" id="CAI50800">
        <property type="protein sequence ID" value="CAI50800"/>
        <property type="gene ID" value="NP_6040A"/>
    </dbReference>
    <dbReference type="GeneID" id="3694688"/>
    <dbReference type="KEGG" id="nph:NP_6040A"/>
    <dbReference type="HOGENOM" id="CLU_010194_1_0_2"/>
    <dbReference type="OrthoDB" id="161871at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Plasmid PL131"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003858">
        <property type="term" value="F:3-hydroxybutyrate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050259">
        <property type="entry name" value="SDR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42879">
        <property type="entry name" value="3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42879:SF2">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] REDUCTASE FABG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAI50800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="289" mass="31250" checksum="D3F12AF178AC7ADB" modified="2005-11-08" version="1">MSSPEAEDSIEPPEITTEVIHRIDDPHFTSNNVALVTGAASGIGRATALVLAANGLTTVATDVDEDGLYNTAQKGEELNVDGEIKPIVCDLTDDAEIEALAETAANAGQLRYVVNVAGMQHVAPIESFPMEMFDRLYQVMLRAPMYITQLALPHIREADDGVGAVANMASVHGHYVTRDKVAYNTMKFGLRGLTKSIAAEGESDIRAFTASTGYVKTPLVLDQIAATAHERDITEREVVEDVMLGQARGGEMMEPIEVGNLFAFGLSRHGKHLNGDDLLWDDGFTNTYE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IME2</accession>
    <name>ASSY_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Argininosuccinate synthase</fullName>
            <ecNumber evidence="1">6.3.4.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Citrulline--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argG</name>
        <name type="ordered locus">NP_5252A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-citrulline + L-aspartate + ATP = 2-(N(omega)-L-arginino)succinate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10932"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the argininosuccinate synthase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.5" evidence="1"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011324326.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
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    <dbReference type="AlphaFoldDB" id="Q3IME2"/>
    <dbReference type="SMR" id="Q3IME2"/>
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        <property type="protein sequence ID" value="CAI50717"/>
        <property type="gene ID" value="NP_5252A"/>
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    <dbReference type="GeneID" id="3702521"/>
    <dbReference type="KEGG" id="nph:NP_5252A"/>
    <dbReference type="eggNOG" id="arCOG00112">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032784_4_0_2"/>
    <dbReference type="OrthoDB" id="5877at2157"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00113"/>
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    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:argininosuccinate synthase activity"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:L-arginine biosynthetic process"/>
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        <property type="term" value="P:urea cycle"/>
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        <property type="entry name" value="ASS"/>
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        <property type="entry name" value="Argininosuccinate synthetase, chain A, domain 2"/>
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        <property type="entry name" value="HUPs"/>
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        <property type="entry name" value="Arginosuc_syn_N"/>
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        <property type="entry name" value="Arginosuc_synth_CS"/>
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        <property type="entry name" value="AS_cat/multimer_dom_body"/>
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        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
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    <dbReference type="NCBIfam" id="TIGR00032">
        <property type="entry name" value="argG"/>
        <property type="match status" value="1"/>
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    <dbReference type="NCBIfam" id="NF001770">
        <property type="entry name" value="PRK00509.1"/>
        <property type="match status" value="1"/>
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    <dbReference type="NCBIfam" id="NF010392">
        <property type="entry name" value="PRK13820.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11587">
        <property type="entry name" value="ARGININOSUCCINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11587:SF2">
        <property type="entry name" value="ARGININOSUCCINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20979">
        <property type="entry name" value="Arginosuc_syn_C"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Arginosuc_synth"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69864">
        <property type="entry name" value="Argininosuccinate synthetase, C-terminal domain"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00564">
        <property type="entry name" value="ARGININOSUCCIN_SYN_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00565">
        <property type="entry name" value="ARGININOSUCCIN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000263998" description="Argininosuccinate synthase">
        <location>
            <begin position="1"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="10"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="123"/>
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        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="124"/>
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        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="127"/>
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        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00005"/>
        </source>
    </evidence>
    <sequence length="400" mass="43861" checksum="CE91113562BE7EBF" modified="2005-11-08" version="1">MSDKNRVALAFSGGLDTTVCVPLLEEEYGYDEVIGVTVDVGQPEAEFKEAEETAEALDLEHHVVDAKPEFAELCFDAVRANASYQGYPLGTALARPVIAEAILEVAKEEGCSGVAHGSTGKGNDQLRFEAVWRDSDLEVIAPVRELGLTRTFEQEYAEERGLPIEGGDEGKYSIDTNLWSRSIEGSELEEPGYVPPEDIYEWTTAPTAETLEVEVGFEDGYPVSLDGEKLEPVELIETLNDLAGDYGVGRTDIMEDRMLGLKVRENYEHPAATVLLNAHQALEDLVLTKEERAFKKQVDHEWSEKGYQGLVNAPLVDALEGFIDETQDRVTGTVTIRFEGGQARPVGRESPYAVYSAEAASFNTEDVTGGIEQSDATGVAKYHGFQERLANRVIDAADEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q3INS8</accession>
    <name>Q3INS8_NATPD</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Succinate dehydrogenase subunit B</fullName>
            <ecNumber evidence="14">1.3.5.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">sdhB</name>
        <name evidence="14" type="ordered locus">NP_4266A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="14"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI50224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323840.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3INS8"/>
    <dbReference type="STRING" id="348780.NP_4266A"/>
    <dbReference type="EnsemblBacteria" id="CAI50224">
        <property type="protein sequence ID" value="CAI50224"/>
        <property type="gene ID" value="NP_4266A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703196"/>
    <dbReference type="KEGG" id="nph:NP_4266A"/>
    <dbReference type="eggNOG" id="arCOG00962">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044838_3_0_2"/>
    <dbReference type="OrthoDB" id="144910at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1060.10:FF:000003">
        <property type="entry name" value="Succinate dehydrogenase iron-sulfur subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000063">
        <property type="entry name" value="Succinate dehydrogenase subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050573">
        <property type="entry name" value="SDH/FRD_Iron-Sulfur"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004489">
        <property type="entry name" value="Succ_DH/fum_Rdtase_Fe-S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025192">
        <property type="entry name" value="Succ_DH/fum_Rdtase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00384">
        <property type="entry name" value="dhsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004616">
        <property type="entry name" value="PRK05950.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11921:SF29">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] IRON-SULFUR SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11921">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE IRON-SULFUR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13085">
        <property type="entry name" value="Fer2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Succinate dehydogenase/fumarate reductase N-terminal" evidence="12">
        <location>
            <begin position="50"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="13">
        <location>
            <begin position="192"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="17"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF13183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAI50224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="287" mass="33498" checksum="28ED49932DAA6318" modified="2005-11-08" version="1">MSTQVTEQEQETEPEQTSEHQERRLSEKRERADMSERAKSDLSDADETVQIKVFRYDPEVEGKKDPRFDTFEIPFTKGMTVLDALIYARDHFDSTLTFRHSCRQAICGSDALFVNGAQRLGCKTQMVDLEWPVRIEPLPHAEVVKDLVVDMEHFYDQMESVEPYFQTNELPDGDLEEQRQTRENREKVKMSTRCIWCSACMSSCNIAAGDNEYLGPAAINKAYRFAMDKREGEEMKQHRLNIIEQEHGVWRCQTQFSCTEVCPKDIPLTEHIQELKREAVKNNLKFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IPL0</accession>
    <name>Q3IPL0_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribonuclease J</fullName>
            <shortName evidence="5">RNase J</shortName>
            <ecNumber evidence="5">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">rnj</name>
        <name evidence="7" type="ordered locus">NP_3698A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">An RNase that has 5'-3' exonuclease activity. May be involved in RNA degradation.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 2 Zn(2+) ions per subunit. It is not clear if Zn(2+) or Mg(2+) is physiologically important.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. Archaeal RNase J subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="5"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323558.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IPL0"/>
    <dbReference type="SMR" id="Q3IPL0"/>
    <dbReference type="STRING" id="348780.NP_3698A"/>
    <dbReference type="EnsemblBacteria" id="CAI49940">
        <property type="protein sequence ID" value="CAI49940"/>
        <property type="gene ID" value="NP_3698A"/>
    </dbReference>
    <dbReference type="GeneID" id="3701841"/>
    <dbReference type="KEGG" id="nph:NP_3698A"/>
    <dbReference type="eggNOG" id="arCOG00546">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008727_4_1_2"/>
    <dbReference type="OrthoDB" id="63419at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07714">
        <property type="entry name" value="RNaseJ_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10710">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01492">
        <property type="entry name" value="RNase_J_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004613">
        <property type="entry name" value="RNase_J"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042173">
        <property type="entry name" value="RNase_J_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030879">
        <property type="entry name" value="RNase_J_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055132">
        <property type="entry name" value="RNase_J_b_CASP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00649">
        <property type="entry name" value="MG423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43694">
        <property type="entry name" value="RIBONUCLEASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43694:SF1">
        <property type="entry name" value="RIBONUCLEASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22505">
        <property type="entry name" value="RNase_J_b_CASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="6">
        <location>
            <begin position="15"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="383"/>
            <end position="387"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="409"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAI49940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="446" mass="49498" checksum="F58928BCE6E0C767" modified="2005-11-08" version="1">MEVEIATIGGYEEVGRQMTAVRAGDNVVIFDMGLNLSKVLIHDNVETERMHSLDLIDMGAIPDDRVMSDIEGDVQAIVPTHGHLDHIGAISKLAHRYDAPIVATPFTIELVKQQIEGEEKFGVQNDLVKMDAGGTMPIGEKCELEFVNVTHSIIDAINPVLHTPEGAVVYGLDKRMDHNPVLGDPIDMDRFREIGREGVLCYIEDCTNAGKKGKTPSESVARSQLKDVMYSLEDYDGGIVATTFSSHIARVKSLVEFAEDIGRQPVLLGRSMEKYSGTAERLDFVDFPSDLGMYGHRKSVDRTFKRIMDEGKEDYLPVVTGHQGEPRAMLTRMARGETPYELDEGDKVIFSARVIPEPTNEGQRYQAEKLLGMQGARIYDDIHVSGHLNSEGHYQMLQALNPTNVIPAHQDMKGFSPYVELASNEGYELGRDLHVTKNGNTIQLTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-11-08" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IQA4</accession>
    <name>Q3IQA4_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribose 1,5-bisphosphate isomerase</fullName>
            <shortName evidence="4">R15P isomerase</shortName>
            <shortName evidence="4">R15Pi</shortName>
            <ecNumber evidence="4">5.3.1.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Ribulose 1,5-bisphosphate synthase</fullName>
            <shortName evidence="4">RuBP synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">NP_3202A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the isomerization of ribose 1,5-bisphosphate (R15P) to ribulose 1,5-bisphosphate (RuBP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>alpha-D-ribose 1,5-bisphosphate = D-ribulose 1,5-bisphosphate</text>
            <dbReference type="Rhea" id="RHEA:32243"/>
            <dbReference type="ChEBI" id="CHEBI:57870"/>
            <dbReference type="ChEBI" id="CHEBI:68688"/>
            <dbReference type="EC" id="5.3.1.29"/>
        </reaction>
    </comment>
    <comment type="miscellaneous">
        <text evidence="4">Reaction proceeds via a cis-phosphoenolate intermediate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eIF-2B alpha/beta/delta subunits family. R15P isomerase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.29" evidence="4"/>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI49692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011323314.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IQA4"/>
    <dbReference type="STRING" id="348780.NP_3202A"/>
    <dbReference type="EnsemblBacteria" id="CAI49692">
        <property type="protein sequence ID" value="CAI49692"/>
        <property type="gene ID" value="NP_3202A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703267"/>
    <dbReference type="KEGG" id="nph:NP_3202A"/>
    <dbReference type="eggNOG" id="arCOG01124">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016218_2_1_2"/>
    <dbReference type="OrthoDB" id="27639at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043917">
        <property type="term" value="F:ribose 1,5-bisphosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046523">
        <property type="term" value="F:S-methyl-5-thioribose-1-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019323">
        <property type="term" value="P:pentose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10470:FF:000019">
        <property type="entry name" value="Ribose 1,5-bisphosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.420">
        <property type="entry name" value="translation initiation factor eif-2b, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02230">
        <property type="entry name" value="R15P_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011559">
        <property type="entry name" value="Initiation_fac_2B_a/b/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027363">
        <property type="entry name" value="M1Pi_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005250">
        <property type="entry name" value="R15Pi"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00524">
        <property type="entry name" value="eIF-2B_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00511">
        <property type="entry name" value="ribulose_e2b2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43475">
        <property type="entry name" value="METHYLTHIORIBOSE-1-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43475:SF2">
        <property type="entry name" value="RIBOSE 1,5-BISPHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2 4 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="311"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="321"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="201"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="24"/>
            <end position="27"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="211"/>
            <end position="212"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAI49692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002698"/>
        </source>
    </evidence>
    <sequence length="334" mass="35235" checksum="EDE3F290AD632589" modified="2005-11-08" version="1">MDETPELHSDVEETARRIAEMEVRGAASIADAAAAALGTQAATAESETPEAFRRTMRAAARRLYETRPTAVSLPNALRYVLSGMDGDDVETLQTTVQQRVSAFRDDLDDAQDRLGQIGAGRFSDGDVVMTHCHSSDALSCIEAAVESGADIEAVVKETRPRKQGHITASELRELGVPVTLIVDSAAHSFLDDVDHVLVGADSIAADGSVINKIGTAGLAVSARDRGVPVVVAAQSIKLHPKTLTGTAVAIERRDIREVLDEETHEDIGAAEVANPAFDVTPPRHVDAIVTEHGQFPPESIVTLMRELFGDSVSEPWTDPDGTGATGASAASGGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q3ITD6</accession>
    <name>RL31_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein eL31</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L31e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl31e</name>
        <name type="ordered locus">NP_1014A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL31 family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011322233.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3ITD6"/>
    <dbReference type="SMR" id="Q3ITD6"/>
    <dbReference type="STRING" id="348780.NP_1014A"/>
    <dbReference type="EnsemblBacteria" id="CAI48598">
        <property type="protein sequence ID" value="CAI48598"/>
        <property type="gene ID" value="NP_1014A"/>
    </dbReference>
    <dbReference type="GeneID" id="3703139"/>
    <dbReference type="KEGG" id="nph:NP_1014A"/>
    <dbReference type="eggNOG" id="arCOG04473">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112570_3_2_2"/>
    <dbReference type="OrthoDB" id="10127at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00463">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00410">
        <property type="entry name" value="Ribosomal_eL31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000054">
        <property type="entry name" value="Ribosomal_eL31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020052">
        <property type="entry name" value="Ribosomal_eL31_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023621">
        <property type="entry name" value="Ribosomal_eL31_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002258">
        <property type="entry name" value="PRK01192.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10956">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10956:SF0">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01198">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01380">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54575">
        <property type="entry name" value="Ribosomal protein L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01144">
        <property type="entry name" value="RIBOSOMAL_L31E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_1000049919" description="Large ribosomal subunit protein eL31">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="90" mass="10135" checksum="D5ECE07D925B4C1A" modified="2005-11-08" version="1">MSAEFDERVMTVPLRDVLAESKGQRADKAMSLVRSHLAQHFNVEEDAVRLDPSINEAVWERGRSKPPSELRVRAARFEEEGEAVVEAETA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-17" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q3IUS7</accession>
    <name>FLUC1_NATPD</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fluoride-specific ion channel FluC 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fluC1</name>
        <name evidence="1" type="synonym">crcB1</name>
        <name type="ordered locus">NP_0024A</name>
    </gene>
    <organism>
        <name type="scientific">Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)</name>
        <name type="synonym">Halobacterium pharaonis</name>
        <dbReference type="NCBI Taxonomy" id="348780"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Stenosarchaea group</taxon>
            <taxon>Halobacteria</taxon>
            <taxon>Halobacteriales</taxon>
            <taxon>Natronomonadaceae</taxon>
            <taxon>Natronomonas</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1336" last="1343">
            <title>Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis.</title>
            <authorList>
                <person name="Falb M."/>
                <person name="Pfeiffer F."/>
                <person name="Palm P."/>
                <person name="Rodewald K."/>
                <person name="Hickmann V."/>
                <person name="Tittor J."/>
                <person name="Oesterhelt D."/>
            </authorList>
            <dbReference type="PubMed" id="16169924"/>
            <dbReference type="DOI" id="10.1101/gr.3952905"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Fluoride-specific ion channel. Important for reducing fluoride concentration in the cell, thus reducing its toxicity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>fluoride(in) = fluoride(out)</text>
            <dbReference type="Rhea" id="RHEA:76159"/>
            <dbReference type="ChEBI" id="CHEBI:17051"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:76160"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the fluoride channel Fluc/FEX (TC 1.A.43) family.</text>
    </comment>
    <dbReference type="EMBL" id="CR936257">
        <property type="protein sequence ID" value="CAI48103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011321742.1">
        <property type="nucleotide sequence ID" value="NC_007426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3IUS7"/>
    <dbReference type="SMR" id="Q3IUS7"/>
    <dbReference type="STRING" id="348780.NP_0024A"/>
    <dbReference type="EnsemblBacteria" id="CAI48103">
        <property type="protein sequence ID" value="CAI48103"/>
        <property type="gene ID" value="NP_0024A"/>
    </dbReference>
    <dbReference type="GeneID" id="3702631"/>
    <dbReference type="KEGG" id="nph:NP_0024A"/>
    <dbReference type="eggNOG" id="arCOG04701">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114342_1_4_2"/>
    <dbReference type="OrthoDB" id="253428at2157"/>
    <dbReference type="Proteomes" id="UP000002698">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062054">
        <property type="term" value="F:fluoride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140114">
        <property type="term" value="P:cellular detoxification of fluoride"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00454">
        <property type="entry name" value="FluC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003691">
        <property type="entry name" value="FluC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28259">
        <property type="entry name" value="FLUORIDE EXPORT PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28259:SF1">
        <property type="entry name" value="FLUORIDE EXPORT PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02537">
        <property type="entry name" value="CRCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0407">Ion channel</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000252967" description="Fluoride-specific ion channel FluC 1">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00454"/>
        </source>
    </evidence>
    <sequence length="119" mass="12393" checksum="C8481F26A8B1BEC9" modified="2005-11-08" version="1">MKPRAVALVAGGGFAGALCRHGIAVVLPGTFPWGTLVVNVAGAFLLGAIVYGTERLRSVPESTRLVVATGFLSSFTTYSTFAGETIALAPRLAALNVVGNYALGFVAVLVAREVIRWRS</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>